Detection of folic acid protein in human serum using reduced graphene oxide electrodes modified by folic-acid.
The detection of disease markers is considered an important step for early diagnosis of cancer. We design in this work a novel electrochemical sensing platform for the sensitive and selective detection of folic acid protein (FP). The platform is fabricated by electrophoretic deposition (EPD) of reduced graphene oxide (rGO) onto a gold electrode and post-functionalization of rGO with folic acid. Upon FP binding, a significant current decrease can be measured using differential pulse voltammetry (DPV). Using this scheme, a detection limit of 1pM is achieved. Importantly, the method also allows the detection of FP in serum being thus an appealing approach for the sensitive detection of biomarkers in clinical samples.
Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR 8520 CNRS, Lille1 University, Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France; Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Lijie
L
He
Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR 8520 CNRS, Lille1 University, Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France; Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials (Ministry of Education), Shandong University, Jinan 250061, China.
Qian
Q
Wang
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Daniel
D
Mandler
Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials (Ministry of Education), Shandong University, Jinan 250061, China.
Musen
M
Li
Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR 8520 CNRS, Lille1 University, Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France.
Rabah
R
Boukherroub
Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR 8520 CNRS, Lille1 University, Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France. Electronic address: sabine.szunerits@iri.univ-lille1.fr.
Sabine
S
Szunerits
D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques; D004566:Electrodes; D005492:Folic Acid / Q000737:chemistry* / Q000378:metabolism; D058972:Folic Acid Transporters / Q000097:blood*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D009369:Neoplasms / Q000097:blood*; D010087:Oxides / Q000737:chemistry
D014408:Biomarkers, Tumor; D058972:Folic Acid Transporters; D010087:Oxides; D006046:Gold; D006108:Graphite; D005492:Folic Acid
Biomarker; Differential pulse voltammetry; Folic acid; Folic acid protein; Reduced graphene oxide