Monocytes and macrophages in flow: an ESCCA initiative on advanced analyses of monocyte lineage using flow cytometry.
In April 2013, a symposium was organized to highlight different aspects of differentiation and activation of the monocyte-macrophage lineage as analyzed on the flow cytometer. Characterization of this lineage requires knowledge of the maturation process from their progenitors that are present in bone marrow up to the mature monocytic cells in peripheral blood, because each monocytic lineage cell with an aberrant phenotype refers to the corresponding maturation stage. A standardized quantitative analysis will facilitate the monitoring of the pathological processes and the clinical features, such as the outcome of treatment. However, changes in marker expression by variation in intensity, asynchronism, and lineage infidelity must be considered. The dynamics of normal marker expressions in early differentiation stages, e.g. molecules like HLA II, CD64 or CD14, give rise to a hypothesis on their possible role in monocyte ontogeny. Besides their usual role in tissue homeostasis, mature macrophages may also play a similar role in hematopoiesis. This meeting highlighted the large potential of flow cytometric tools available for monitoring of all these aspects in the monocytic and macrophage cell lineage. © 2015 International Clinical Cytometry Society.
Immunology Laboratory, CNRS UMR5307 Labo Georges Friedel (LGF), Pole De Biologie-Pathologie, University Hospital, St Etienne, France.
Claude
C
Lambert
Department of Laboratory Medicine Laboratory of Hematology, Radboud University Medical Center, Nijmegen, The Netherlands.
Frank W M B
FWMB
Preijers
Department of Immunology, Yeditepe University School of Medicine, Istanbul, Turkey.
Gulderen
G
Yanikkaya Demirel
Institute of Clinical Immunology, Medical Faculty; Translational Centre for Regenerative Medicine (TRM), Universität Leipzig, Leipzig, Germany.
Ulrich
U
Sack
D002454:Cell Differentiation; D019070:Cell Lineage; D005434:Flow Cytometry; D006801:Humans; D008264:Macrophages / Q000166:cytology*; D009000:Monocytes / Q000166:cytology*
bactericidy; cell signaling; flow cytometry; macrophage; monocytes; myelodysplasia; phosphor-flow