Anticancer activities of self-assembled molecular bowls containing a phenanthrene-based donor and Ru(II) acceptors.
Nano-sized multinuclear ruthenium complexes have rapidly emerged as promising therapeutic candidates with unique anticancer activities. Here, we describe the coordination-driven self-assembly and anticancer activities of a set of three organometallic tetranuclear Ru(II) molecular bowls. [2+2] Coordination-driven self-assembly of 3, 6-bis(pyridin-3- ylethynyl) phenanthrene (bpep) (1) and one of the three dinuclear arene ruthenium clips, [(η6-p-iPrC6H4Me)2Ru2-(OO\OO)][OTf]2 (OO\OO =2, 5-dioxido-1, 4-benzoquinonato, OTf = triflate) (2), 5, 8-dioxido-1, 4-naphthoquinonato (3), or 6, 11-dioxido-5, 12-naphthacenediona (4), resulted in three molecular bowls 5-7 of general formula [{(η6-p-iPrC6H4Me)2Ru2-(OO\OO)}2(bpep)2][OTf]4. All molecular bowls were obtained as triflate salts in very good yields (>90%) and were fully characterized using multinuclear nuclear magnetic resonance (NMR), electrospray ionization-mass spectrometry (ESI-MS), and elemental analysis. The structure of the representative molecular bowl 5 was confirmed by single-crystal X-ray diffraction analysis. The anticancer activities of molecular bowls 5-7 were determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide, autophagy, and Western blot analysis. Bowl 6 showed the strongest cytotoxicity in AGS human gastric carcinoma cells and was more cytotoxic than doxorubicin. In addition, autophagic activity and the ratio of apoptotic cell death increased in AGS cells by treatment with bowl 6. Bowl 6 also induced autophagosome formation via upregulation of p62 and promotion of the conversion of LC3-I to LC3-II. Moreover, bowl 6 promoted apoptotic cell death through downregulation of Akt/mTOR activation, followed by increased caspase-3 activity. These results suggest that bowl 6 induces gastric cancer cell death via modulation of autophagy and apoptosis. Bowl 6 is a potent anticancer agent and a potential treatment for human gastric cancer that merits further study.
Laboratory of Bio-Resources, Yongin-si, Gyeonggi-Do, Republic of Korea.
Inhye
I
Kim
Department of Chemistry, University of Ulsan, Ulsan, Republic of Korea.
Young Ho
YH
Song
Department of Chemistry, University of Ulsan, Ulsan, Republic of Korea.
Nem
N
Singh
Department of Life Science, Gachon University, Seongnam, Republic of Korea.
Yong Joon
YJ
Jeong
Department of Life Science, Gachon University, Seongnam, Republic of Korea.
Jung Eun
JE
Kwon
Energy Materials Lab, Korea Institute of Energy Research, Daejeon, Republic of Korea.
Hyunuk
H
Kim
Department of Life Science, Gachon University, Seongnam, Republic of Korea.
Young Mi
YM
Cho
Department of Life Science, Gachon University, Seongnam, Republic of Korea.
Se Chan
SC
Kang
Department of Chemistry, University of Ulsan, Ulsan, Republic of Korea.
Ki-Whan
KW
Chi
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D010616:Phenanthrenes / Q000737:chemistry / Q000494:pharmacology; D012428:Ruthenium / Q000737:chemistry / Q000494:pharmacology
D000970:Antineoplastic Agents; D010616:Phenanthrenes; C031181:phenanthrene; D012428:Ruthenium
Akt/mTOR; coordination self-assembly; gastric cancer; phenanthrene; ruthenium