Specific distribution of non-phosphorylated neurofilaments characterizing each subfield in the mouse auditory cortex.
Recent imaging studies revealed the presence of functional subfields in the mouse auditory cortex. However, little is known regarding the morphological basis underlying the functional differentiation. Distribution of particular molecules is the key information that may be applicable for identifying auditory subfields in the post-mortem brain. Immunoreactive patterns using SMI-32 monoclonal antibody against non-phosphorylated neurofilament (NNF) have already been used to identify or parcellate various brain regions in various animals. In the present study, we investigated whether distribution of NNF is a reliable marker for identifying functional subfields in the mouse auditory cortex, and found that each auditory subfield has region-specific cellular and laminar patterns of immunoreactivity for NNF.
Division of Neurobiology and Anatomy, Graduate School of Medical and Dental Sciences. Electronic address: mhorie@med.niigata-u.ac.jp.
Masao
M
Horie
Department of Neurophysiology, Brain Research Institute, Niigata University, Japan.
Hiroaki
H
Tsukano
Division of Neurobiology and Anatomy, Graduate School of Medical and Dental Sciences.
Hirohide
H
Takebayashi
Department of Neurophysiology, Brain Research Institute, Niigata University, Japan.
Katsuei
K
Shibuki
D000161:Acoustic Stimulation; D000818:Animals; D001303:Auditory Cortex / Q000033:anatomy & histology / Q000378:metabolism*; D008297:Male; D008810:Mice, Inbred C57BL; D016900:Neurofilament Proteins / Q000378:metabolism*
D016900:Neurofilament Proteins
Auditory cortex; Biotinylated dextran amine; Flavoprotein fluorescence imaging; Mouse; Non-phosphorylated neurofilament