Genetic basis of keloid formation in wounds after cardiac surgery.
Keloid disease is the abnormal formation of scar tissue in genetically predisposed people. Among many genes which may be related to the development of keloids, transforming growth factor β1 (TGF-β1) is one of the most mentioned. It encodes cytokinin, which is responsible for the production of extracellular matrix and takes part in healing. Any abnormalities which arise during synthesis of the protein as a result of polymorphism or gene mutation may be the cause of healing disorders (scarring of the body); thus it is responsible for the development of keloids. The objective of this study is to determine the single nucleotide polymorphism of the gene TGF-β1, at the position -509(rs1800469)509, to compare the obtained results in the form of three different genotypes within the analysed group (keloids) and within the control group (healthy scars), and to analyse the correlation between obtained genotypes and the occurrence of keloid disease. Seventy-three patients after cardiac surgery with scars on their sternums were examined (22 women and 51 men) in the age group from 38 to 84 years. Two groups of patients were distinguished: 37 with keloids and 36 with healthy scars. DNA taken from patients was analysed and polymorphism C(-509)T of the gene TGF-β1 was determined. On the basis of the study it was found that the allele T in the position -509 of the gene TGF-β1 is associated with a lower risk of keloid formation regardless of age and gender.
Clinic of Plastic Surgery, Endocrine and General Surgery, Pomeranian Medical University in Szczecin, Poland.
Przemysław
P
Kulawczuk
Clinic of Plastic Surgery, Endocrine and General Surgery, Pomeranian Medical University in Szczecin, Poland.
Norbert
N
Czapla
Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University in Szczecin, Poland.
Agnieszka
A
Bińczak-Kuleta
Department of Biochemistry, Department of Biochemistry and Medical Chemistry, Pomeranian Medical University in Szczecin, Poland.
Krzysztof
K
Safranow
Clinic of Haematology, Pomeranian Medical University in Szczecin, Poland.
Anna
A
Jaworska-Kulawczuk
Students Research Circle at Clinic of Plastic Surgery, Endocrine and General Surgery, Pomeranian Medical University in Szczecin, Poland.
Dominika
D
Gajewska
Students Research Circle at Clinic of Plastic Surgery, Endocrine and General Surgery, Pomeranian Medical University in Szczecin, Poland.
Karolina
K
Agata
Students Research Circle at Clinic of Plastic Surgery, Endocrine and General Surgery, Pomeranian Medical University in Szczecin, Poland.
Miłosz
M
Brykczyński
Students Research Circle at Clinic of Plastic Surgery, Endocrine and General Surgery, Pomeranian Medical University in Szczecin, Poland.
Piotr
P
Bargiel
gene mutation; gene polymorphism; keloid disease; keloid scarring