Effects of Cuminum cyminum L. essential oil on some epididymal sperm parameters and histopathology of testes following experimentally induced copper poisoning in mice.
Copper overload can cause sperm cell damage by inducing oxidative stress. On the other hand, cumin has a good antioxidant potential. Therefore, the aim of this study was to evaluate the effects of cumin on sperm quality and testicular tissue following experimentally induced copper poisoning in mice. Forty-eight mature male mice were divided into four equal groups as follows: group Cu which received 0.1 ml copper sulphate at dose of 100 mg kg(-1) , group Cc which received Cuminum cyminum at dose of 1 mg kg(-1) , treatment group which received copper sulphate (100 mg kg(-1) ) and treated with Cuminum cyminum (1 mg kg(-1) ), and control group which received the same volume of normal saline. Six mice in each group were sacrificed at week 4 and week 6. The results showed that sperm concentration, motility and viability in group Cu were significantly decreased at weeks 4 and 6, and severe degenerative changes were observed in testicular tissues in comparison with the control group. In treatment group, significant improvement in the sperm count, motility and viability, and normal architecture in most seminiferous tubules with organised epithelium was observed compared to the group Cu. The sperm quality parameters in the treatment group approached those of the control group.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
E
E
Sakhaee
Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
L
L
Emadi
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
O
O
Azari
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
R
R
Kheirandish
Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
M R
MR
Esmaili Nejad
Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
H
H
Shafiei Bafti
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D003300:Copper / Q000506:poisoning*; D028527:Cuminum; D004822:Epididymis / Q000187:drug effects; D008297:Male; D051379:Mice; D009822:Oils, Volatile / Q000494:pharmacology*; D055101:Semen Analysis; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000473:pathology*
D000975:Antioxidants; D009822:Oils, Volatile; D003300:Copper
Copper toxicosis; Cuminum cyminum; mice; sperm quality; testes