N-ethylmaleimide-sensitive factor siRNA improves cardiac function following myocardial infarction in rats.
This study examined the effects of N-ethylmaleimide-sensitive factor (NSF) small interfering RNA (siRNA) on cardiac function following myocardial infarction (MI) in rats. Thirty-six adult Sprague Dawley rats were randomly divided into three equivalent groups. An acute MI model was established by ligating the anterior descending branch of the left coronary artery and confirmed by electrocardiogram. Recombinant NSF-siRNA adenovirus (experimental), negative adenovirus (control), and normal saline were injected near the infarcted area of the left ventricle in each respective group. The left ventricular ejection fraction (LVEF) was measured with a noninvasive ultrasonic cardiogram. Left ventricular end-diastolic pressure (LVEDP) and the maximum rate of rise in left ventricular pressure (+dp/dt max) were measured using the BL-420 Biological Functional Experimental System. Hearts were sectioned and stained with 2,3,5,-triphenyl tetrazolium chloride (TTC) to observe the MI area. Two weeks after surgery, LVEF in the experimental group (46.0 ± 7.5%) was higher than control (34.0 ± 6.0%) and saline (37.5 ± 4.5%) group LVEFs (P < 0.05), whereas LVEDP was the lowest in the experimental group (18.51 ± 6.87 vs 29.47 ± 9.94 and 26.58 ± 8.97 mmHg, respectively) (P < 0.05). The +dp/dt max was also higher in the experimental group (9.74 ± 1.16 vs 4.33 ± 1.19 and 5.24 ± 1.53 mmHg/s x 10(3), respectively) (P < 0.05); however, the MI area did not differ significantly between groups. Local injection of an adenovirus-mediated NSF-siRNA expression vector near infarcted areas improved cardiac function two weeks after MI, but had no impact on the MI area.
Emergency Department, Peking University Ninth School of Clinical Medicine, Beijing, China.
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Y
Zhou
Emergency Department, The 302 Hospital of PLA, Beijing, China.
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Y
Liu
Department of Cardiology, Peking University Ninth School of Clinical Medicine, Beijing, China.
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SX
Yang
Emergency Department, Peking University Ninth School of Clinical Medicine, Beijing, China.
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Wang
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D004195:Disease Models, Animal; D004562:Electrocardiography; D005822:Genetic Vectors / Q000235:genetics; D008297:Male; D009203:Myocardial Infarction / Q000175:diagnosis / Q000235:genetics* / Q000503:physiopathology*; D052066:N-Ethylmaleimide-Sensitive Proteins / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D051381:Rats; D013318:Stroke Volume; D016277:Ventricular Function, Left; D020257:Ventricular Remodeling
D034741:RNA, Small Interfering; D052066:N-Ethylmaleimide-Sensitive Proteins