Development and Quality Control of Nanohexaconazole as an Effective Fungicide and Its Biosafety Studies on Soil Nitifiers.
The study was aimed to develop a nano form of an existing fungicide for improving plant protection and reducing crop losses caused by fungal pathogens. The protocol for the preparation and estimation of nanohexaconazole was developed. Technically pure hexaconazole was converted into its nanoform using polyethyleneglycol-400 (PEG) as the surface stabilizing agent. Nanohexaconazole was characterized using Scanning Electron Microscopy (SEM) and Dynamic Light Scattering (DLS) studies. The average particle size of nanohexaconazole was about 100 nm. An analytical method was also developed for quality control of the nanofungicide by GLC fitted with flame ionization detector. Its limit of detection was 2.5 ppm. Fungicidal potential of nanohexaconazole was better in comparison to that of conventional hexaconazole. Hydrolytic and thermal stability studies confirmed its stability at par with the conventional formulation of fungicide. Impact of nanohexaconazole on soil nitrifiers was tested in vitro and there were no significant adverse effect in their numbers observed as compared to conventional registered formulation, proving the safety of the nanofungicide.
Rajesh
R
Kumar
Kishore Kumar
KK
Nair
Md Imteyaz
MI
Alam
Robin
R
Gogoi
Pradeep Kumar
PK
Singh
Chitra
C
Srivastava
Madhuban
M
Gopal
Arunava
A
Goswami
D002470:Cell Survival / Q000187:drug effects; D004339:Drug Compounding / Q000592:standards*; D005659:Fungicides, Industrial / Q000008:administration & dosage / Q000138:chemical synthesis / Q000633:toxicity; D007194:India; D008422:Materials Testing; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000633:toxicity; D058465:Nitrification / Q000502:physiology; D009576:Nitrobacter / Q000187:drug effects* / Q000502:physiology; D009606:Nitrosomonas / Q000187:drug effects* / Q000502:physiology; D010316:Particle Size; D011786:Quality Control; D012988:Soil Microbiology; D014230:Triazoles / Q000008:administration & dosage* / Q000138:chemical synthesis* / Q000633:toxicity
D005659:Fungicides, Industrial; D014230:Triazoles; C409722:hexaconazole