Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana.
Aleurites moluccana L. is grown as a roadside tree in southern China and the oil content of its seed is higher than other oil plants, such as Jatropha curcas and Camellia oleifera. A. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. In addition, the bark, leaves, and kernels of A. moluccana have various medical and commercial uses. Here, a novel gene coding the biotin carboxyl carrier protein subunit (BCCP) was cloned from A. moluccana L. using the homology cloning method combined with rapid amplification of cDNA end (RACE) technology. The isolated full-length cDNA sequence (designated AM-accB) was 1188 bp, containing a 795-bp open reading frame coding for 265 amino acids. The deduced amino acid sequence of AM-accB contained a biotinylated domain located between amino acids 190 and 263. A. moluccana BCCP shows high identity at the amino acid level to its homologues in other higher plants, such as Vernicia fordii, J. curcas, and Ricinus communis (86, 77, and 70%, respectively), which all contain conserved domains for ACCase activity. The expression of the AM-accB gene during the middle stage of development and maturation in A. moluccana seeds was higher than that in early and later stages. The expression pattern of the AM-accB gene is very similar to that of the oil accumulation rate.
Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, China.
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WY
Xuan
Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, China.
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Y
Zhang
Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, China.
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ZQ
Liu
Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, China.
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D
Feng
Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, China.
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Luo
D000103:Acetyl-CoA Carboxylase / Q000737:chemistry / Q000235:genetics*; D031284:Aleurites / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D054889:Fatty Acid Synthase, Type II / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D010938:Plant Oils / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013997:Time Factors
D018076:DNA, Complementary; D010938:Plant Oils; D021122:Protein Subunits; D054889:Fatty Acid Synthase, Type II; D000103:Acetyl-CoA Carboxylase; C079633:biotin carboxyl carrier protein