Protective effects on vascular endothelial cell in N'-nitro-L-arginine (L-NNA)-induced hypertensive rats from the combination of effective components of Uncaria rhynchophylla and Semen Raphani.
Endothelial dysfunction is closely associated with hypertension. Protection of vascular endothelial cell is the key to prevention and treatment of hypertension. Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid, isolated from traditional Chinese medicine Uncaria rbyncbopbylla and Semen Raphani respectively, exhibit properties of anti-hypertension and protection of blood vessels. In the present study, we observed the protective effect of the combined use of Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid to the vascular endothelial cell in N'-nitro-L-arginine-induced hypertensive rats and investigate the preliminary mechanism. Blood pressure was detected by non-invasive rats tail method to observe the anti-hypertension effect of drugs. Scanning electron microscopy was used to observe the integrity or shedding state of vascular endothelial cell. The amount of circulating endothelial cells and CD54 and CD62P expression on circulating endothelial cells were tested to evaluate the endothelium function. In this study, we found that the Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid compatibility can effectively lower the blood pressure, improve the structural integrity of vascular endothelium, and significantly reduce the number of circulating endothelial cells. Furthermore, the mean fluorescence intensity of CD54 and CD62P expressed showed decrease after the intervention of Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid compatibility. In conclusion, the combination of effective components of the Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid demonstrated good antihypertension effect and vascular endothelium protective effect. The preliminary mechanism of the protective effect may attribute to relieve the overall low-grade inflammation.
Affiliated Hospital of Shandong University of Traditional Chinese Medicine.
Yunlun
Y
Li
Wenqing
W
Yang
Qingjun
Q
Zhu
Jinguo
J
Yang
Zhen
Z
Wang
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D019607:Brassicaceae / Q000737:chemistry*; D002452:Cell Count; D048430:Cell Shape / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D006973:Hypertension / Q000139:chemically induced* / Q000188:drug therapy* / Q000503:physiopathology; D008297:Male; D019335:Nitroarginine; D008517:Phytotherapy; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D011921:Rats, Inbred WKY; D032070:Uncaria / Q000737:chemistry*
D015703:Antigens, CD; D020011:Protective Agents; D019335:Nitroarginine