Investigation of single nucleotide polymorphisms in phosphodiesterase 4D gene in Mongol and Han patients with ischemic stroke in Inner Mongolia.
We investigated single nucleotide polymorphisms (SNP) at 87 sites of the phosphodiesterase 4D (PDE4D) gene in Mongol and Han patients with ischemic stroke in Inner Mongolia. SNPs in 226 patients with ischemic stroke (case group, 110 Mongol patients, 116 Han patients) and 220 patients without neurological disease (control group, 102 Mongol patients, 118 Han patients) were detected by polymerase chain reaction-restriction fragment length polymorphism and gene sequencing. The genotype and allele frequencies of all groups were compared. There were no statistically significant differences in genotypes in the PDE4D gene at 87 sites between the case and control groups (P > 0.05). The C allele frequency in the case group was significantly higher than that in the control group (P < 0.05). The CC genotype and C allele frequencies in the Mongol case subgroup were higher than those in the Mongol control subgroup (P < 0.05). The CC genotype and C allele frequencies in the Han case subgroup were higher than those in the Han control subgroup (P < 0.05). In the case group, there were no significant differences at 87 sites for genotypes and allele frequencies between the Mongol and Han subgroups. In the control group, there were no significant differences at 87 site genotypes and allele frequencies between the Mongol and Han subgroups. The increase in the C allele frequency at 87 SNP sites in PDE4D may increase ischemic stroke risk. We found no differences in the risk between Mongol and Han populations in Inner Mongolia.
Chinese Internal Medicine Teaching and Researching Section, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
J P
JP
Shi
Chinese Internal Medicine Teaching and Researching Section, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
W D
WD
Chen
Internal Medicine Section, No. 253 Hospital of PLA, Hohhot, Inner Mongolia, China.
J Q
JQ
Zhou
Chinese Internal Medicine Teaching and Researching Section, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
M M
MM
Xue
Chinese Internal Medicine Teaching and Researching Section, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
F
F
Xue
Chinese Internal Medicine Teaching and Researching Section, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
H Z
HZ
Li
Chinese Internal Medicine Teaching and Researching Section, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Z P
ZP
Xu
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D002545:Brain Ischemia / Q000150:complications / Q000201:enzymology / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4 / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D020521:Stroke / Q000150:complications / Q000201:enzymology / Q000235:genetics*
D054703:Cyclic Nucleotide Phosphodiesterases, Type 4