Blu-ray based optomagnetic aptasensor for detection of small molecules.
This paper describes an aptamer-based optomagnetic biosensor for detection of a small molecule based on target binding-induced inhibition of magnetic nanoparticle (MNP) clustering. For the detection of a target small molecule, two mutually exclusive binding reactions (aptamer-target binding and aptamer-DNA linker hybridization) are designed. An aptamer specific to the target and a DNA linker complementary to a part of the aptamer sequence are immobilized onto separate MNPs. Hybridization of the DNA linker and the aptamer induces formation of MNP clusters. The target-to-aptamer binding on MNPs prior to the addition of linker-functionalized MNPs significantly hinders the hybridization reaction, thus reducing the degree of MNP clustering. The clustering state, which is thus related to the target concentration, is then quantitatively determined by an optomagnetic readout technique that provides the hydrodynamic size distribution of MNPs and their clusters. A commercial Blu-ray optical pickup unit is used for optical signal acquisition, which enables the establishment of a low-cost and miniaturized biosensing platform. Experimental results show that the degree of MNP clustering correlates well with the concentration of a target small molecule, adenosine triphosphate (ATP) in this work, in the range between 10µM and 10mM. This successful proof-of-concept indicates that our optomagnetic aptasensor can be further developed as a low-cost biosensing platform for detection of small molecule biomarkers in an out-of-lab setting.
Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, DK-2800 Kongens Lyngby, Denmark; Department of Mechanical Engineering, Columbia University, New York, NY 10027, United States.
Jaeyoung
J
Yang
Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, DK-2800 Kongens Lyngby, Denmark.
Marco
M
Donolato
POLYMAT, University of the Basque Country UPV/EHU, 20018 Donostia-San Sebastián, Spain.
Alessandro
A
Pinto
Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, DK-2800 Kongens Lyngby, Denmark.
Filippo Giacomo
FG
Bosco
Institute of Physics, Academia Sinica, Nankang, Taipei 11529, Taiwan.
En-Te
ET
Hwu
Institute of Physics, Academia Sinica, Nankang, Taipei 11529, Taiwan.
Ching-Hsiu
CH
Chen
Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, DK-2800 Kongens Lyngby, Denmark.
Tommy Sonne
TS
Alstrøm
Department of Materials Science and Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
Gwan-Hyoung
GH
Lee
POLYMAT, University of the Basque Country UPV/EHU, 20018 Donostia-San Sebastián, Spain; IKERBASQUE, Basque Foundation for Science, 48011 Bilbao, Spain.
Thomas
T
Schäfer
IKERBASQUE, Basque Foundation for Science, 48011 Bilbao, Spain; CIC nanoGUNE Consolider, 20018 Donostia-San Sebastián, Spain.
Paolo
P
Vavassori
Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, DK-2800 Kongens Lyngby, Denmark.
Anja
A
Boisen
Department of Mechanical Engineering, Columbia University, New York, NY 10027, United States. Electronic address: qlin@columbia.edu.
Qiao
Q
Lin
Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, DK-2800 Kongens Lyngby, Denmark. Electronic address: mikkel.hansen@nanotech.dtu.dk.
Mikkel Fougt
MF
Hansen
D000255:Adenosine Triphosphate / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015415:Biomarkers / Q000737:chemistry*; D015374:Biosensing Techniques; D015342:DNA Probes / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D015415:Biomarkers; D015342:DNA Probes; D058185:Magnetite Nanoparticles; D006046:Gold; D000255:Adenosine Triphosphate
Aptamer; Blu-ray; Inhibition assay; Magnetic nanoparticles; Optomagnetic readout