Purification, characterization and antitumor activity of polysaccharides extracted from Phellinus igniarius mycelia.
Water-soluble intracellular polysaccharides (IPS) were extracted from cultured mycelia of Phellinus igniarius. The IPS were purified by ethanol fractional precipitation, ion-exchange and size exclusion chromatography in that order. Homogeneous polysaccharide IPSW-1, IPSW-2, IPSW-3, and IPSW-4 were obtained, which molecular characteristics were examined using multiangle laser-light scattering and refractive index detector system. The average molecular weights of them were 34.1, 17.7, 15.1, 21.7kDa, respectively. GC analysis indicated that IPSW-1, IPSW-2 and IPSW-3 all only contained glucose, while IPSW-4 was composed of rhamnose, xylose, mannose, glucose and galactose in a molar ratio of 1.29:1.21:1:43.86:1.86. UV and IR analysis suggested they belonged to α-type of the pyran group and didn't contain protein. These homogeneous polysaccharides could inhibit the growth of SW480 and HepG2 cells to a certain extent in a dose-dependent manner. So they could be beneficial for the further development of a natural carcinoma preventive agent and functional food.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Shi-Chao
SC
Li
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: XM_Yang1963@126.com.
Xiao-Ming
XM
Yang
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Hai-Le
HL
Ma
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Jing-Kun
JK
Yan
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Dan-Zhao
DZ
Guo
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D045744:Cell Line, Tumor; D000431:Ethanol / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D025282:Mycelium / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry
D000970:Antineoplastic Agents; D062610:Fungal Polysaccharides; D009005:Monosaccharides; D014867:Water; D000431:Ethanol
Antitumor activity; Phellinus igniarius mycelia; Polysaccharide; Purification