Effects of Tongmai oral liquid in femoral ateriovenous fistula.
BACKGROUND
This study was conducted to investigate the protective effect of Tongmai oral liquid on arteriovenous fistula function and to provide an effective method to promote fistula maturation.


METHODS
Fifteen female and fifteen male SPF New Zealand rabbits were randomly allocated into 3 groups including control, Aspirin and Tongmai oral liquid groups. A side-to-side femoral arteriovenous fistula was established in each rabbit and then animals were treated with Aspirin or Tongmai oral liquid for 2 weeks. The concentrations of circulating ET-1 and NO were determined before and after operation (on preoperative day, operative day, post-D1, post-D3, post-D7 and post-D15), respectively. Blood flow of the fistula stoma and contralateral artery and vein was determined on the 15th postoperative day. Last, the fistula stoma was dissected to observe patency, thrombosis and adhesion with surrounding tissues.


RESULTS
28 rabbits survived during the surgical process and the following 15-day observational period. Tissue adhesion of arteriovenous fistula with surrounding tissues was improved and fistula thrombosis was reduced by treatment with Tongmai oral liquid. NO concentration decreased to a different extent after vascular surgery. Tongmai oral liquid failed to regulate the equilibrium between NO and ET-1, but it improved blood flow of fistula stoma, as compared to control and Aspirin groups. Blood flow of fistula stoma in the three groups was lower than that of the contralateral femoral artery.


CONCLUSIONS
Tongmai oral liquid improved the function of femoral ateriovenous fistula in the rabbit model by increasing blood flow and reducing thrombosis, probably not by regulating the dynamic equilibrium between NO and ET-1.
Department of Nephrology, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), No.111 of Dade Road, Guangzhou, 510120, China. su_peiling_ok@126.com.|Department of Nephrology, The Third Affiliated Hospital, Guangxi University of Chinese Medicine (Liuzhou traditional Chinese Medical Hospital), No.32 of Jiefang Road, Liuzhou, 545001, China. su_peiling_ok@126.com.
Pei-Ling
PL
Su
Department of Nephrology, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), No.111 of Dade Road, Guangzhou, 510120, China. baokun@aliyun.com.
Kun
K
Bao
Department of Nephrology, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), No.111 of Dade Road, Guangzhou, 510120, China. myspecter@163.com.|Jiangmen Xinhui District Hospital of Chinese Medicine, No.47 of Huimin Road, Jiangmen, 529100, China. myspecter@163.com.
Han-Guo
HG
Peng
Department of Nephrology, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), No.111 of Dade Road, Guangzhou, 510120, China. maowei1274@126.com.
Wei
W
Mao
Department of Nephrology, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), No.111 of Dade Road, Guangzhou, 510120, China. 120346352@qq.com.
Guan-Su
GS
Wang
Department of Nephrology, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), No.111 of Dade Road, Guangzhou, 510120, China. gzynz0018@163.com.
Ni-Zhi
NZ
Yang
Department of Nephrology, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), No.111 of Dade Road, Guangzhou, 510120, China. angle11023@163.com.
Wen-Jia
WJ
Geng
Department of Nephrology, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), No.111 of Dade Road, Guangzhou, 510120, China. linyiqun2000@163.com.
Yi-Qun
YQ
Lin
Department of Nephrology, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), No.111 of Dade Road, Guangzhou, 510120, China. jiexina@163.com.
Xi-Na
XN
Jie
D000818:Animals; D001164:Arteriovenous Fistula; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005263:Femoral Artery / Q000002:abnormalities / Q000187:drug effects; D008297:Male; D011817:Rabbits; D012039:Regional Blood Flow / Q000187:drug effects
D004365:Drugs, Chinese Herbal