Diagnostic value of dual detection of hepatocyte nuclear factor 1 beta (HNF-1β) and napsin A for diagnosing ovarian clear cell carcinoma.
We evaluated the diagnostic value of hepatocyte nuclear factor 1 beta (HNF-1β) and napsin A for diagnosing ovarian clear cell carcinoma. Immunohistochemical EnVision was used to measure HNF-1β and napsin A expression in 38 cases of ovarian clear cell carcinoma, 30 cases of high-grade serous carcinoma, 22 cases of endometrioid adenocarcinoma, and 16 metastatic Krukenberg tumor cases. Then we found that HNF-1β appeared in all ovarian clear cell carcinoma and was less common in high-grade serous and endometrioid adenocarcinoma (P < 0.05). However, no significant difference in HNF-1β between clear cell carcinoma and metastatic Krukenberg tumor was found (P > 0.05). Napsin A was expressed in 97.4% of ovarian clear cell carcinoma, 6.7% high-grade serous carcinoma, 22.7% endometrioid adenocarcinoma, and 0% metastatic Krukenberg tumors. Napsin A in clear cell carcinoma was greater than that found in high-grade serous carcinoma, endometrioid adenocarcinoma, and metastatic Krukenberg tumor (P < 0.05). Sensitivity and specificity of HNF-1β and napsin A for diagnosing ovarian clear cell carcinoma was 100% and 54.4%, and 97.4% and 89.7%, respectively. Sensitivity and specificity of HNF-1β and napsin A for diagnosing ovarian clear cell carcinoma was 97.4% and 91.2%, respectively. So it is concluded that HNF-1β and napsin A are more sensitive than currently used markers for diagnosing ovarian clear cell carcinoma. Moreover, napsin A is more specific than HNF-1β. Combining HNF-1β and napsin A may distinguish clear cell carcinoma from high-grade serous carcinoma, endometrioid adenocarcinoma and metastatic Krukenberg tumors.
Department of Pathology, Shanghai Pudong New Area People's Hospital Shanghai 201299, China.
Qing
Q
Li
Department of Pathology, Nanjing Maternity and Child Health Hospital Affiliated to Nanjing Medical University Nanjing 210004, China.
Xin
X
Zeng
Department of Pathology, Nanjing Maternity and Child Health Hospital Affiliated to Nanjing Medical University Nanjing 210004, China.
Xue
X
Cheng
Department of Pathology, Nanjing Maternity and Child Health Hospital Affiliated to Nanjing Medical University Nanjing 210004, China.
Jingmin
J
Zhang
Department of Pathology, Nanjing Maternity and Child Health Hospital Affiliated to Nanjing Medical University Nanjing 210004, China.
Jie
J
Ji
Department of Pathology, Nanjing Hospital (Nanjing First Hospital), Nanjing Medical University Nanjing 210006, China.
Jinsong
J
Wang
Department of Pathology, Nanjing Hospital (Nanjing First Hospital), Nanjing Medical University Nanjing 210006, China.
Kemei
K
Xiong
Department of Pathology, Nanjing Hospital (Nanjing First Hospital), Nanjing Medical University Nanjing 210006, China.
Qiong
Q
Qi
Department of Pathology, Nanjing Hospital (Nanjing First Hospital), Nanjing Medical University Nanjing 210006, China.
Wenbin
W
Huang
D016282:Aspartic Acid Endopeptidases / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D018269:Carcinoma, Endometrioid / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D003937:Diagnosis, Differential; D005260:Female; D051539:Hepatocyte Nuclear Factor 1-beta / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007725:Krukenberg Tumor / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D060787:Neoplasm Grading; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D011237:Predictive Value of Tests
D014408:Biomarkers, Tumor; C496495:HNF1B protein, human; D051539:Hepatocyte Nuclear Factor 1-beta; D016282:Aspartic Acid Endopeptidases; C498549:NAPSA protein, human
Ovarian neoplasm; clear cell carcinoma; endometrioid adenocarcinoma; hepatocyte nuclear factor 1 beta (HNF-1β); napsin A; serous carcinoma