Cell-adhesive RGD peptide-displaying M13 bacteriophage/PLGA nanofiber matrices for growth of fibroblasts.
BACKGROUND
M13 bacteriophages can be readily fabricated as nanofibers due to non-toxic bacterial virus with a nanofiber-like shape. In the present study, we prepared hybrid nanofiber matrices composed of poly(lactic-co-glycolic acid, PLGA) and M13 bacteriophages which were genetically modified to display the RGD peptide on their surface (RGD-M13 phage).


RESULTS
The surface morphology and chemical composition of hybrid nanofiber matrices were characterized by scanning electron microscopy (SEM) and Raman spectroscopy, respectively. Immunofluorescence staining was conducted to investigate the existence of M13 bacteriophages in RGD-M13 phage/PLGA hybrid nanofibers. In addition, the attachment and proliferation of three different types of fibroblasts on RGD-M13 phage/PLGA nanofiber matrices were evaluated to explore how fibroblasts interact with these matrices. SEM images showed that RGD-M13 phage/PLGA hybrid matrices had the non-woven porous structure, quite similar to that of natural extracellular matrices, having an average fiber diameter of about 190 nm. Immunofluorescence images and Raman spectra revealed that RGD-M13 phages were homogeneously distributed in entire matrices. Moreover, the attachment and proliferation of fibroblasts cultured on RGD-M13 phage/PLGA matrices were significantly enhanced due to enriched RGD moieties on hybrid matrices.


CONCLUSIONS
These results suggest that RGD-M13 phage/PLGA matrices can be efficiently used as biomimetic scaffolds for tissue engineering applications.
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 609-735 Korea.
Yong Cheol
YC
Shin
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 609-735 Korea.
Jong Ho
JH
Lee
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 609-735 Korea.
Linhua
L
Jin
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 609-735 Korea.
Min Jeong
MJ
Kim
Department of Nanomaterials Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan, 609-735 Korea.
Jin-Woo
JW
Oh
Department of Design, College of Arts, Pusan National University, Busan, 609-735 Korea.
Tai Wan
TW
Kim
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 609-735 Korea.
Dong-Wook
DW
Han
Extracellular matrices; Fibroblasts; M13 bacteriophages; PLGA nanofibers; RGD peptide