[THE ROLE OF HYDROGEN SULFIDE IN VOLUME-DEPENDENT MECHANISMS OF REGULATION OF VASCULAR SMOOTH MUSCLE CELLS CONTRACTILE ACTIVITY].
The hydrogen sulfide (H2S) influence on the contractile activity of vascular smooth muscle cells (SMC) was studied on endothelium-denuded aortic ring segments of male Wistar rats with method of mechanography. Contractions of SMS were induced by incubation in high potassium solution as well as in hyper-, hypo- and isosmotic solutions. 5-100 LM of H2S donor--sodium hydrosulfide (NaHS) increased mechanical tension of SMC precontracted with high potassium solution that was abolished by bumetanide--the inhibitor of Na+, K+, 2Cl(-) -cotransporter (NKCC), but 100-1000 microM of NaHS relaxed SMS. NaHS (10 microM) increased the amplitude of hyper- and isosmotic contraction, but not of hyposmotic contraction. NaHS (ImM) decreased the amplitude of hyper-, iso-, and hyposmotic contractions. The direct measurements of NKCC activity with radionuclide method showed an increase in NKCC activity under the action of 5-100 microM of NaHS. These findings suggest that low concentrations of H2S participate in the NKCC activation. This mechanism underlines constrictive action of H2S on smooth muscle cells.
L V
LV
Smagliy
S V
SV
Gusakova
Yu G
YG
Birulina
I V
IV
Kovalev
S N
SN
Orlov
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002034:Bumetanide / Q000494:pharmacology; D048429:Cell Size / Q000187:drug effects; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D006982:Hypertonic Solutions; D007038:Hypotonic Solutions; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects*; D009129:Muscle Tonus / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D017208:Rats, Wistar; D064466:Solute Carrier Family 12, Member 1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014661:Vasoconstriction / Q000187:drug effects*
D006982:Hypertonic Solutions; D007038:Hypotonic Solutions; C578716:Slc12a1 protein, rat; D064466:Solute Carrier Family 12, Member 1; D002034:Bumetanide; D006862:Hydrogen Sulfide