Molecular Characterization and Serology of Leptospira kirschneri (Serogroup Grippotyphosa) Isolated from Urine of a Mare Post-Abortion in Brazil.
A strain of Leptospira kirschneri (serogroup Grippotyphosa) was cultured from urine of a mare post-abortion in Brazil and characterized by serogrouping, multiple-locus variable-number tandem repeat analysis, PGFE, and sequencing of genes rrs and secY. Strains of L. kirschneri have apparently never been recovered from horses in tropical area, only in Europe and USA. Knowledge of local epidemiology is important to interpret genetic profiles of leptospires circulating in an area.
Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói, RJ, Brazil.
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Hamond
Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói, RJ, Brazil.
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Martins
Unité de Biologie des Spirochètes, Institut Pasteur, National Reference Center, Paris, France.
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Bremont
Bio-Manguinhos, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Rio de Janeiro, RJ, Brazil.
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Medeiros
Unité de Biologie des Spirochètes, Institut Pasteur, National Reference Center, Paris, France.
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Bourhy
Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói, RJ, Brazil.
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Lilenbaum
D000034:Abortion, Veterinary; D000818:Animals; D057138:Bacterial Shedding; D015373:Bacterial Typing Techniques; D001938:Brazil / Q000453:epidemiology; D005260:Female; D006734:Horse Diseases / Q000453:epidemiology / Q000382:microbiology*; D006736:Horses; D007919:Leptospira / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D007922:Leptospirosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D011247:Pregnancy; D011634:Public Health; D017422:Sequence Analysis, DNA / Q000662:veterinary; D065288:Serogroup; D014556:Urine / Q000382:microbiology; D015047:Zoonoses
Leptospira kirschneri; grippotyphosa; horse