Grafted biomembranes containing membrane proteins--the case of the leucine transporter.
Here, we bind the sodium dependent amino acid transporter on nitrilotriacetic acid/polyethylene glycol functionalized gold sensors in detergents and perform a detergent-lipid exchange with phosphatidylcholine. We characterize the LeuT structure in the adsorbed film by magnetic contrast neutron reflection using the predicted model from molecular dynamic simulations.
Department of Chemistry and Nano-Science Center, University of Copenhagen, Universitetsparken 5, DK 2100, Copenhagen, Denmark. cardenas@nano.ku.dk.
Vivien
V
Jagalski
Robert D
RD
Barker
Mikkel B
MB
Thygesen
Kamil
K
Gotfryd
Mie B
MB
Krüger
Lei
L
Shi
Selma
S
Maric
Nicolas
N
Bovet
Martine
M
Moulin
Michael
M
Haertlein
Thomas Günther
TG
Pomorski
Claus J
CJ
Loland
Marité
M
Cárdenas
D026905:Amino Acid Transport Systems / Q000737:chemistry / Q000378:metabolism*; D003902:Detergents / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D056004:Molecular Dynamics Simulation; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques; D012964:Sodium / Q000737:chemistry
D026905:Amino Acid Transport Systems; D003902:Detergents; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D011092:Polyethylene Glycols; D006046:Gold; D012964:Sodium; D009571:Nitrilotriacetic Acid