An early post-traumatic reaction of lymph-heart striated muscle fibers in adult frog Rana temporaria during the first postoperative week: An electron microscopic and autoradiographic study.
According to the current opinion, lymph-heart striated muscle represents a specialized type of skeletal muscles in frogs. Here, we studied muscle fibers in mechanically damaged lymph hearts during the first postoperative week using electron-microscopic autoradiography. We present evidence that both, the satellite cells and pre-existing muscle fibers bordering the site of injury, contribute directly to the lymph-heart muscle regeneration. Several muscle fibers located in the vicinity of the damaged area displayed features of nuclear and sarcoplasmic activation. We also observed ultrastructural changes indicating activation of a few satellite cells, namely decondensation of chromatin, enlargement of nuclei and nucleoli, appearance of free ribosomes and rough endoplasmic reticulum tubules in the cytoplasm. Electron-microscopic autoradiography showed that 4 h after single (3)H-thymidine administration on the seventh day after injury not only the activated satellite cells, but also some nuclei of myofibers bordering the injured zone are labeled. We showed that both, the myonuclei of fibers displaying the signs of degenerative/reparative processes in the sarcoplasm and the myonuclei of the fibers enriched with highly organized myofibrils, can re-enter into the S-phase. Our results indicate that the nuclei of lymph-heart myofibers can reactivate DNA synthesis during regenerative myogenesis, unlike the situation in regenerating frog skeletal muscle where myogenic cells do not synthesize DNA at the onset of myofibrillogenesis.
Lab. of Cell Morphology, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, 194064, Russia.
Marina I
MI
Krylova
Lab. of Cell Morphology, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, 194064, Russia.
Dmitry S
DS
Bogolyubov
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D042601:Lymphatic Vessels / Q000166:cytology; D024510:Muscle Development; D054792:Muscle, Striated / Q000000981:diagnostic imaging / Q000502:physiology / Q000648:ultrastructure*; D011859:Radiography; D011896:Rana temporaria; D012038:Regeneration
electron-microscopic autoradiography; frog; lymph-heart striated muscle; regeneration; satellite cells