Reliability of the Lactate Scout point-of-care instrument for the determination of blood L-lactate concentration in sheep.
BACKGROUND
Data on accuracy and precision of the Lactate Scout point-of-care (POC) analyzer in ovine medicine are lacking.


OBJECTIVE
The purpose of the study was to assess the reliability of the Lactate Scout in sheep.


METHODS
Fifty-seven sheep at varying ages with various diseases were included. Blood lactate concentration in samples collected from the jugular vein was measured immediately on the Lactate Scout. Plasma L-lactate concentration was measured by the Cobas autoanalyzer as the reference method. Data were subjected to Student's t-test, Passing-Bablok regression, and Bland-Altman plot analyses for comparison and assessment of accuracy, precision, and reliability.


RESULTS
Plasma l-lactate concentration was consistently lower than blood L-lactate concentration (3.06 ± 0.24 vs 3.3 ± 0.3 mmol/L, P < .0001). There was a positive correlation between plasma and blood L-lactate concentrations (r = .98, P < .0001). The Lactate Scout had 99% accuracy and 98% precision with the reference method. Blood (Y) and plasma (X) L-lactate concentrations were fitted to Y = 0.28 + 1.00 · X, with a residual standard deviation of 0.31 and a negligible deviation from the identity line (P = .93). The bias was fitted to Y = 0.10 + 0.05 · X, with Sy.x of 0.44 (P < .07).


CONCLUSIONS
The Lactate Scout has high accuracy and precision, with a negligible bias. It is a reliable POC analyzer to assess L-lactate concentration in ovine medicine.
Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey.
Ozgur
O
Kaynar
Department of Internal Medicine, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey.
Tolga
T
Karapinar
Department of Animal Nutrition and Nutritional Disorders, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey.
Armagan
A
Hayirli
Department of Internal Medicine, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey.
Ersoy
E
Baydar
D000818:Animals; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000662:veterinary*; D007773:Lactates / Q000097:blood*; D019095:Point-of-Care Systems; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep / Q000097:blood*
D007773:Lactates
Accuracy; bias; lactate meter; ovine medicine; precision