Laccase activity is proportional to the abundance of bacterial laccase-like genes in soil from subtropical arable land.
Laccase enzymes produced by both soil bacteria and fungi play important roles in refractory organic matter turnover in terrestrial ecosystems. We investigated the abundance and diversity of fungal laccase genes and bacterial laccase-like genes in soil from subtropical arable lands, and identified which microbial group was associated with laccase activity. Compared with fungal laccase genes, the bacterial laccase-like genes had greater abundance, richness and Shannon-Wiener diversity. More importantly, laccase activity can be explained almost exclusively by the bacterial laccase-like genes, and their abundance had significant linear relationship with laccase activity. Thus, bacterial laccase-like gene has great potential to be used as a sensitive indicator of laccase enzyme for refractory organic matter turnover in subtropical arable lands.
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100039, People's Republic of China.|Huanjiang Observation and Research Station for Karst Ecosystems, Huanjiang, 547100, People's Republic of China.
Shuzhen
S
Feng
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, People's Republic of China.|Huanjiang Observation and Research Station for Karst Ecosystems, Huanjiang, 547100, People's Republic of China.|ISA-CAS and UWA Joint Laboratory for Soil Systems Biology, Changsha, 410125, People's Republic of China.
Yirong
Y
Su
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100039, People's Republic of China.|Huanjiang Observation and Research Station for Karst Ecosystems, Huanjiang, 547100, People's Republic of China.
Mingzhe
M
Dong
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, People's Republic of China.|Huanjiang Observation and Research Station for Karst Ecosystems, Huanjiang, 547100, People's Republic of China.
Xunyang
X
He
ISA-CAS and UWA Joint Laboratory for Soil Systems Biology, Changsha, 410125, People's Republic of China.|School of Earth and Environment, The University of Western Australia, Crawley, WA, 6009, Australia.
Deepak
D
Kumaresan
ISA-CAS and UWA Joint Laboratory for Soil Systems Biology, Changsha, 410125, People's Republic of China.|School of Earth and Environment, The University of Western Australia, Crawley, WA, 6009, Australia.
Anthony G
AG
O'Donnell
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, People's Republic of China.|ISA-CAS and UWA Joint Laboratory for Soil Systems Biology, Changsha, 410125, People's Republic of China.
Jinshui
J
Wu
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, People's Republic of China. xbchen@isa.ac.cn.|Huanjiang Observation and Research Station for Karst Ecosystems, Huanjiang, 547100, People's Republic of China. xbchen@isa.ac.cn.|ISA-CAS and UWA Joint Laboratory for Soil Systems Biology, Changsha, 410125, People's Republic of China. xbchen@isa.ac.cn.|Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Changsha, 410128, People's Republic of China. xbchen@isa.ac.cn.
Xiangbi
X
Chen
D001419:Bacteria / Q000201:enzymology* / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D017753:Ecosystem; D005658:Fungi / Q000201:enzymology* / Q000235:genetics*; D014644:Genetic Variation; D042845:Laccase / Q000235:genetics* / Q000378:metabolism*; D005810:Multigene Family; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004271:DNA, Fungal; D012987:Soil; D042845:Laccase
Arable land; Bacteria; Fungi; Laccase