Exploration of Energy Metabolism in the Mouse Using Indirect Calorimetry: Measurement of Daily Energy Expenditure (DEE) and Basal Metabolic Rate (BMR).
Current comprehensive mouse metabolic phenotyping involves studying energy balance in cohorts of mice via indirect calorimetry, which determines heat release from changes in respiratory air composition. Here, we describe the measurement of daily energy expenditure (DEE) and basal metabolic rate (BMR) in mice. These well-defined metabolic descriptors serve as meaningful first-line read-outs for metabolic phenotyping and should be reported when exploring energy expenditure in mice. For further guidance, the issue of appropriate sample sizes and the frequency of sampling of metabolic measurements is also discussed.
Institute for Diabetes and Obesity, Helmholtz-Zentrum München GmbH, Neuherberg, Germany.
Carola W
CW
Meyer
Institute for Health Economics and Health Care Management, Helmholtz-Zentrum München GmbH, Neuherberg, Germany.
Peter
P
Reitmeir
Institute for Diabetes and Obesity, Helmholtz-Zentrum München GmbH, Neuherberg, Germany.|Division of Metabolic Diseases, Department of Medicine, Technische Universität München, Munich, Germany.
Matthias H
MH
Tschöp
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001481:Basal Metabolism; D002153:Calorimetry, Indirect / Q000295:instrumentation / Q000379:methods*; D004734:Energy Metabolism; D051379:Mice / Q000378:metabolism*
basal metabolic rate; daily energy expenditure; indirect calorimetry; mouse phenotyping