(1→6)- and (1→3)(1→6)-β-glucans from Lasiodiplodia theobromae MMBJ: Structural characterization and pro-inflammatory activity.
The chemical composition and structural characterization of exopolysaccharides from the fungus Lasiodiplodia theobromae MMBJ are described, and the immunomodulatory activity of a purified β-glucan was evaluated. L. theobromae MMBJ produced three different β-glucans. One, fraction PEPS, was a branched (1→3)(1→6)-β-glucan and was insoluble in cold water. The other two, fractions SEPS-005R and SEPS-10E, were characterized as linear (1→6)-β-glucans with molar mass of 1.8×10(6)Da and 7.0×10(3)Da, respectively. From a total of 2.2g/L of EPS produced by L. theobromae through submerged fermentation, 1.5g/L (67%) was of the branched (1→3)(1→6)-β-glucan, while 25% (w/w) were linear (1→6)-β-glucans. Tests conducted with macrophages showed that the high molar mass (1→6)-β-glucan fraction (SEPS-005R) induced a pro-inflammatory response pattern.
Departamento de Ciências Biológicas, Universidade Estadual Paulista - UNESP (FCL-Assis), CEP 19806-900 Assis, SP, Brazil.
Kassandra S M
KS
Oliveira
Departamento de Ciências Biológicas, Universidade Estadual Paulista - UNESP (FCL-Assis), CEP 19806-900 Assis, SP, Brazil.
Mirela
M
Di Bastiani
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980 Curitiba, PR, Brazil. Electronic address: lucimaramcc@ufpr.br.
Lucimara M C
LM
Cordeiro
Departamento de Ciências Biológicas, Universidade Estadual Paulista - UNESP (FCL-Assis), CEP 19806-900 Assis, SP, Brazil.
Mírian F
MF
Costa
Departamento de Ciências Biológicas, Universidade Estadual Paulista - UNESP (FCL-Assis), CEP 19806-900 Assis, SP, Brazil.
Karina A
KA
Toledo
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980 Curitiba, PR, Brazil.
Marcello
M
Iacomini
Departamento de Química, Universidade Estadual de Londrina, CEP 86051-990 Londrina, PR, Brazil.
Aneli M
AM
Babosa
Biorefining and Biotechnology Consultancy, Rua João Huss 200, Gleba Palhano, CEP 86050-490 Londrina, PR, Brazil.
Robert F H
RF
Dekker
Departamento de Ciências Biológicas, Universidade Estadual Paulista - UNESP (FCL-Assis), CEP 19806-900 Assis, SP, Brazil.
Valéria M G
VM
Nascimento
D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D001203:Ascomycota / Q000737:chemistry* / Q000378:metabolism; D002240:Carbohydrate Sequence; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D005285:Fermentation; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D008969:Molecular Sequence Data; D047071:beta-Glucans / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D047071:beta-Glucans
(1→3)(1→6)-β-Glucan; Exopolysaccharides; Immunomodulatory activity; Lasiodiplodan; Linear (1→6)-β-glucan