Exploring the effects of treatments with carbohydrases to obtain a high-cellulose content pulp from a non-wood alkaline pulp.
In this work, treatments with a xylanase (X) and carbohydrases mixture (Cx) were applied on a TCF bleached sisal pulp in order to obtain high-cellulose content fibers applicable on a wide range of uses. A limit of ≈12% w/w final content in hemicelluloses was found regardless of the enzymatic treatment assessed. An extraction with 4% and 9% w/v NaOH was performed for further hemicelluloses removal. We found that NaOH dose could be strongly reduced if combined with Cx or Cx+X treatments. Also, if necessary, a stronger reduction could be obtained with 9% w/v NaOH, which was found to be boosted in a 14% if performed after a treatment with Cx. An end-product with a low content in xylans (≈2.9% w/w) and in HexA (5.8μmol/odp) was obtained. Pulp Fock solubility was also increased (≈30%) by enzymatic treatments. HPLC analysis of effluents provided useful information of enzymatic catalytic mechanisms.
CELBIOTECH_Paper Engineering Research Group, Universitat Politècnica de Catalunya (UPC. BarcelonaTech), Colom 11, E-08222 Terrassa, Spain.
Facundo
F
Beltramino
CELBIOTECH_Paper Engineering Research Group, Universitat Politècnica de Catalunya (UPC. BarcelonaTech), Colom 11, E-08222 Terrassa, Spain.
Cristina
C
Valls
CELBIOTECH_Paper Engineering Research Group, Universitat Politècnica de Catalunya (UPC. BarcelonaTech), Colom 11, E-08222 Terrassa, Spain.
Teresa
T
Vidal
CELBIOTECH_Paper Engineering Research Group, Universitat Politècnica de Catalunya (UPC. BarcelonaTech), Colom 11, E-08222 Terrassa, Spain. Electronic address: roncero@etp.upc.edu.
M Blanca
MB
Roncero
D027882:Agave / Q000737:chemistry; D055162:Biocatalysis; D002482:Cellulose / Q000737:chemistry* / Q000302:isolation & purification; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D010209:Paper; D012972:Sodium Hydroxide / Q000737:chemistry
D012972:Sodium Hydroxide; D002482:Cellulose; D006026:Glycoside Hydrolases; C019822:carbohydrase; D043364:Endo-1,4-beta Xylanases
Carbohydrase; Endoglucanase; Enzymatic treatment; High-cellulose content; Non-wood pulp; Xylanase