Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel.
Cellulosome is a kind of multienzyme complex that displays high activity, selectivity, and stability. Here, we report a novel, non-cellulolytic, cellulosome-like multienzyme complex that produced by the Cellulosimicrobium cellulans wild-type strain F16 isolated from soil microflora. This multienzyme complex, with excellent catalytic efficiency of kcat 13.2 s(-1) to remove the C-7 xylosyl group from 7-xylosyl-10-deacetylpaclitaxel (10-DAXP), has an outstanding tolerance against organic solvents and an excellent general stability, with the long half-life of 214 hours. This cellulosome-like multienzyme complex has a novel structure distinct from the well-documented ones. The key catalytic subunit responsible for the β-xylosidase activity against 10-DAXP is identified to be a novel protein, indicating a new glycoside hydrolase (GH) family. The pioneering work described here offers a novel nanoscale biocatalyst for the production of biofuels and chemicals from renewable plant-based natural resources.
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Tong-Yi
TY
Dou
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Hong-Wei
HW
Luan
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Guang-Bo
GB
Ge
Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Ming-Ming
MM
Dong
Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Han-Fa
HF
Zou
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Yu-Qi
YQ
He
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Pan
P
Cui
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Jia-Yue
JY
Wang
Biotechnology Institute, School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China.
Da-Cheng
DC
Hao
Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Shi-Lin
SL
Yang
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.|Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Ling
L
Yang
D000595:Amino Acid Sequence; D002384:Catalysis; D044603:Cellulosomes / Q000378:metabolism*; D004789:Enzyme Activation; D004795:Enzyme Stability; D016680:Genome, Bacterial; D023281:Genomics; D006027:Glycosides / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017239:Paclitaxel / Q000031:analogs & derivatives / Q000378:metabolism; D017433:Protein Structure, Secondary; D021122:Protein Subunits; D016415:Sequence Alignment; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
C531111:7-xylosyl-10-deacetylpaclitaxel; D006027:Glycosides; D009097:Multienzyme Complexes; D021122:Protein Subunits; D017239:Paclitaxel