Rapid identification and quantitative analysis of the chemical constituents in Scutellaria indica L. by UHPLC-QTOF-MS and UHPLC-MS/MS.
Ultra high performance liquid chromatography (UHPLC) coupled with a hybrid quadrupole time-of-flight mass spectrometry (QTOF-MS) was used to separate and identify the compounds in Scutellaria indica L. Fragmentation patterns of these compounds were investigated by the UHPLC-QTOF-MS technique in both negative and positive ion modes. By using this strategy, both intact precursors and fragment ions information were obtained from a single injection. A total of 42 phenolic and other compounds were unequivocally or tentatively identified from S. indica for the first time. Among them, 8 phenolic compounds: scutellarin, luteolin, naringenin, wogonoside, apigenin, hispidulin, wogonin and chrysin were further quantified and used as marker substances by multiple-reaction monitoring in negative ionization mode. The results demonstrated that the calibration curves for all analytes showed good linearity (R(2)>0.9992) within the test ranges. The overall limits of detection and limits of quantification were 0.12-10.52 ng/mL and 4.67-20.22 ng/mL, respectively. The recovery was 96.02-102.88% with a relative standard deviation of less than 3.02%. It is proposed that the methods described here can be applied for rapid evaluation, quality control and authenticity establishment of S. indica.
National Engineering Institute for the Research and Development of Endangered Medicinal Resources in Southwest China, Guangxi Botanical Garden of Medicinal Plants, Guangxi Province, China.
Lili
L
He
Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu 610041, Sichuan Province, China. Electronic address: zfzhang25@163.com.
Zhifeng
Z
Zhang
Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu 610041, Sichuan Province, China.
Luyang
L
Lu
Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu 610041, Sichuan Province, China.
Yuan
Y
Liu
Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu 610041, Sichuan Province, China.
Shan
S
Li
School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangdong Province, China.
Jiangang
J
Wang
National Engineering Institute for the Research and Development of Endangered Medicinal Resources in Southwest China, Guangxi Botanical Garden of Medicinal Plants, Guangxi Province, China.
Zhijun
Z
Song
National Engineering Institute for the Research and Development of Endangered Medicinal Resources in Southwest China, Guangxi Botanical Garden of Medicinal Plants, Guangxi Province, China.
Zhigang
Z
Yan
National Engineering Institute for the Research and Development of Endangered Medicinal Resources in Southwest China, Guangxi Botanical Garden of Medicinal Plants, Guangxi Province, China. Electronic address: mjh1962@vip.163.com.
Jianhua
J
Miao
D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D031363:Scutellaria; D053719:Tandem Mass Spectrometry / Q000379:methods / Q000592:standards*; D013997:Time Factors
D004365:Drugs, Chinese Herbal
Chemical compositions; Scutellaria indica; Scutellarin; UHPLC–MS/MS; UHPLC–QTOF–MS