Transcriptome analysis of the Chinese bread wheat cultivar Yunong 201 and its ethyl methanesulfonate mutant line.
Roche 454 next-generation sequencing was applied to obtain extensive information about the transcriptomes of the bread wheat cultivar Yunong 201 and its EMS mutant line Yunong 3114. Totals of 1.43 million and 1.44 million raw reads were generated, 14,432, 17,845 and 27,867 isotigs were constructed using the reads in Yunong 201, Yunong 3114 and their combination, respectively. Moreover, 29,042, 34,722, and 48,486 unigenes were generated in Yunong 201, Yunong 3114, and combined cultivars, respectively. A total of 50,382 and 59,891 unigenes from the Yunong 201 and Yunong 3114 were mapped on different chromosomes. Of all unigenes, 1363 DEGs were identified in Yunong 201 and Yunong 3114. qRT-PCR analysis confirmed the expression profiles of 40 candidate unigenes possibly related to abiotic stresses. The expression patterns of four annotated DEGs were also verified in the two wheat cultivars under abiotic stresses. This study provided useful information for further analysis of wheat functional genomics.
Agronomy College, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Corn Crop, Henan Agricultural University, Zhengzhou 450002, China. Electronic address: zhangning88012@126.com.
Ning
N
Zhang
Agronomy College, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Corn Crop, Henan Agricultural University, Zhengzhou 450002, China. Electronic address: 515605313@qq.com.
Shasha
S
Wang
Agronomy College, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Corn Crop, Henan Agricultural University, Zhengzhou 450002, China. Electronic address: 799578937@qq.com.
Xiangfen
X
Zhang
Agronomy College, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Corn Crop, Henan Agricultural University, Zhengzhou 450002, China. Electronic address: dongzhongdong@163.com.
Zhongdong
Z
Dong
Agronomy College, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Corn Crop, Henan Agricultural University, Zhengzhou 450002, China. Electronic address: chf0088@163.com.
Feng
F
Chen
Agronomy College, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Corn Crop, Henan Agricultural University, Zhengzhou 450002, China. Electronic address: cdq62@sohu.com.
Dangqun
D
Cui
D002681:China; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D018506:Gene Expression Regulation, Plant; D016296:Mutagenesis / Q000187:drug effects; D009154:Mutation; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D013312:Stress, Physiological; D059467:Transcriptome; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
D010940:Plant Proteins; D005020:Ethyl Methanesulfonate
EMS mutant line; RNA-seq; Transcriptome; Wheat; qRT-PCR