Role of Hairpin-Quadruplex DNA Secondary Structural Conversion in the Promoter of hnRNP K in Gene Transcriptional Regulation.
The promoter of hnRNP K oncogene was found to contain a G/C-rich sequence on the same DNA strand, which can form interconvertible G-quadruplex, i-motif, and hairpin structures. Protein CNBP could bind and stabilize the G-quadruplex, inducing transformation of the hairpin into the G-quadruplex, resulting in down-regulation of hnRNP K transcription. In contrast, Corticosterone could bind and stabilize the hairpin, inducing transformation of the G-quadruplex into the hairpin, resulting in up-regulation of hnRNP K gene transcription.
School of Pharmaceutical Sciences, Sun Yat-sen University , Guangzhou University City, 132 Waihuan East Road, Guangzhou, Guangdong 510006, P. R. China.
Jun
J
Qiu
School of Pharmaceutical Sciences, Sun Yat-sen University , Guangzhou University City, 132 Waihuan East Road, Guangzhou, Guangdong 510006, P. R. China.
Jinggong
J
Liu
School of Pharmaceutical Sciences, Sun Yat-sen University , Guangzhou University City, 132 Waihuan East Road, Guangzhou, Guangdong 510006, P. R. China.
Shuobin
S
Chen
School of Pharmaceutical Sciences, Sun Yat-sen University , Guangzhou University City, 132 Waihuan East Road, Guangzhou, Guangdong 510006, P. R. China.
Tian-Miao
TM
Ou
School of Pharmaceutical Sciences, Sun Yat-sen University , Guangzhou University City, 132 Waihuan East Road, Guangzhou, Guangdong 510006, P. R. China.
Jia-Heng
JH
Tan
School of Pharmaceutical Sciences, Sun Yat-sen University , Guangzhou University City, 132 Waihuan East Road, Guangzhou, Guangdong 510006, P. R. China.
Lian-Quan
LQ
Gu
School of Pharmaceutical Sciences, Sun Yat-sen University , Guangzhou University City, 132 Waihuan East Road, Guangzhou, Guangdong 510006, P. R. China.
Zhi-Shu
ZS
Huang
School of Pharmaceutical Sciences, Sun Yat-sen University , Guangzhou University City, 132 Waihuan East Road, Guangzhou, Guangdong 510006, P. R. China.
Ding
D
Li
D001483:Base Sequence; D003596:Cytosine / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry; D034641:Heterogeneous-Nuclear Ribonucleoprotein K / Q000235:genetics*; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D016601:RNA-Binding Proteins / Q000378:metabolism; D050436:Regulatory Elements, Transcriptional
C433227:CNBP protein, human; D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D016601:RNA-Binding Proteins; D006147:Guanine; D003596:Cytosine; D004247:DNA