Charged solvatochromic dyes as signal transducers in pH independent fluorescent and colorimetric ion selective nanosensors.
Ionophore-based ion selective optical nanosensors that operate independently of the sample pH are developed here by the use of electrically charged solvatochromic dyes as signal transducers. A series of dye molecules with a D-π-A structure was synthesized and characterized in various solvents and incorporated into ion selective nanospheres for K(+), Na(+), and H(+). Since dye leakage was greatly suppressed when the solvatochromic dyes were encapsulated in the nanosphere core, ion sensing nanospheres were explored for cellular ion imaging in Dictyostelium discoideum live cells but spontaneous dye loss resulted in undesired staining of cells. The in vitro analysis of potassium in human plasma was successfully demonstrated with this approach. A theoretical model was developed for the response of the ion selective nanosensors containing charged solvatochromic dyes. The nanosensors exhibited a tunable response range, high sensitivity, and good stability.
Department of Inorganic and Analytical Chemistry, University of Geneva , Quai Ernest-Ansermet 30, CH-1211, Geneva, Switzerland.
Xiaojiang
X
Xie
Department of Inorganic and Analytical Chemistry, University of Geneva , Quai Ernest-Ansermet 30, CH-1211, Geneva, Switzerland.|Department of Biochemistry, University of Geneva , Quai Ernest-Ansermet 30, CH-1211, Geneva, Switzerland.
Agustín
A
Gutiérrez
Department of Biochemistry, University of Geneva , Quai Ernest-Ansermet 30, CH-1211, Geneva, Switzerland.
Valentin
V
Trofimov
Department of Inorganic and Analytical Chemistry, University of Geneva , Quai Ernest-Ansermet 30, CH-1211, Geneva, Switzerland.
Istvan
I
Szilagyi
Department of Biochemistry, University of Geneva , Quai Ernest-Ansermet 30, CH-1211, Geneva, Switzerland.
Thierry
T
Soldati
Department of Inorganic and Analytical Chemistry, University of Geneva , Quai Ernest-Ansermet 30, CH-1211, Geneva, Switzerland.
Eric
E
Bakker
D002412:Cations / Q000032:analysis; D003124:Colorimetry / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry*; D004023:Dictyostelium / Q000737:chemistry / Q000166:cytology; D005453:Fluorescence; D006801:Humans; D006859:Hydrogen / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D054118:Nanospheres / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D061848:Optical Imaging; D010949:Plasma / Q000737:chemistry; D011188:Potassium / Q000032:analysis* / Q000097:blood; D012964:Sodium / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods
D002412:Cations; D004396:Coloring Agents; D006859:Hydrogen; D012964:Sodium; D011188:Potassium