A novel reflectance-based aptasensor using gold nanoparticles for the detection of oxytetracycline.
We present a novel reflectance-based colorimetric aptasensor using gold nanoparticles for the detection of oxytetracycline for the first time. It was found that the reflectance-based measurement at two wavelengths (650 and 520 nm) can generate more stable and sensitive signals than absorbance-based sensors to determine the aggregation of AuNPs, even at high AuNP concentrations. One of the most common antibacterial agents, oxytetracycline (OTC), was detected at concentrations as low as 1 nM in both buffer solution and tap water, which was 25-fold more sensitive, compared to the previous absorbance-based colorimetric aptasensors. This reflectance-based colorimetric aptasensor using gold nanoparticles is considered to be a better platform for portable sensing of small molecules using aptamers.
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-Gu, Seoul 136-713, Republic of Korea. mbgu@korea.ac.kr.
Ho Bin
HB
Seo
Young Seop
YS
Kwon
Ji-eun
JE
Lee
David
D
Cullen
Hongseok Moses
HM
Noh
Man Bock
MB
Gu
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D010118:Oxytetracycline / Q000032:analysis* / Q000737:chemistry
D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D006046:Gold; D010118:Oxytetracycline