Dietary polyunsaturated fatty acids from flaxseed affect immune responses of dairy sheep around parturition.
The objective of the study was to characterize the immune profile of dairy ewes fed flaxseed, rich in polyunsaturated fatty acids (PUFA), around parturition. The hypothesis to be verified was that a physiological stressor, such as parturition, could be overcome with a nutritional manipulation in the diet of the animal in order to guarantee welfare of animals and to sustain their immune responses. Twenty Comisana ewes were divided in two groups (10 ewes/group), and fed a supplementation of whole flaxseed in the diet (FS group) or no supplementation (CON group). Blood samples were collected at parturition and then 7, 14, 21, 28, and 42 day post partum. Plasma samples were used to assess the humoral immune response after ovalbumin (OVA) immunization. At parturition, at 14 day, and 42 day post partum the level of plasma cytokines was assessed. The sheep showed a reduced responsiveness to OVA immunization. In FS ewes the IL-6 level remained unchanged until 14 day post partum and then significantly decreased from 14 day to 42 day post partum. IL-10 level was significantly higher in FS ewes than in CON ewes at 14 day. At parturition IL-1β level was significantly lower in FS ewes than in CON ewes and significantly decreased in both groups from parturition to 42 day. In conclusion, PUFA from flaxseed, as supplement in the diet of ewes around parturition can modulate sheep immune reactivity by influencing cytokine production.
Department of the Sciences of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli, 25, 71122 Foggia, Italy. Electronic address: mariangela.caroprese@unifg.it.
Mariangela
M
Caroprese
Department of the Sciences of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli, 25, 71122 Foggia, Italy.
Maria Giovanna
MG
Ciliberti
Department of the Sciences of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli, 25, 71122 Foggia, Italy.
Marzia
M
Albenzio
CRA - Consiglio per la Ricerca e la sperimentazione in Agricoltura, Unità di ricerca per la zootecnia estensiva (ZOE), Segezia, Foggia, Italy.
Giovanni
G
Annicchiarico
Department of the Sciences of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli, 25, 71122 Foggia, Italy.
Agostino
A
Sevi
D000818:Animals; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D019587:Dietary Supplements / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage* / Q000737:chemistry; D005260:Female; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008043:Linseed Oil / Q000008:administration & dosage* / Q000737:chemistry; D010047:Ovalbumin / Q000276:immunology; D036801:Parturition / Q000276:immunology*; D049590:Postpartum Period / Q000276:immunology*; D011247:Pregnancy; D034561:Sheep, Domestic / Q000276:immunology*
D004042:Dietary Fats, Unsaturated; D005231:Fatty Acids, Unsaturated; D007074:Immunoglobulin G; D053583:Interleukin-1beta; D015850:Interleukin-6; D016753:Interleukin-10; D008043:Linseed Oil; D010047:Ovalbumin
Dairy sheep; Flaxseed; Immune function; Parturition; Polyunsaturated fatty acid