Evaluation of dual-wavelength excitation autofluorescence imaging of colorectal tumours with a high-sensitivity CMOS imager: a cross-sectional study.
BACKGROUND
It is important to devise efficient and easy methods of detecting colorectal tumours to reduce mortality from colorectal cancer. Dual-wavelength excitation autofluorescence intensity can be used to visualize colorectal tumours. Therefore, we evaluated dual-wavelength excitation autofluorescence images of colorectal tumours obtained with a newly developed, high-sensitivity complementary metal-oxide-semiconductor (CMOS) imager.


METHODS
A total 107 colorectal tumours (44 adenomas, 43 adenocarcinomas with intramucosal invasion, and 20 sessile serrated adenoma/polyps [SSA/Ps]) in 98 patients who underwent endoscopic tumour resection were included. The specimens were irradiated with excitation light at 365 nm and 405 nm, and autofluorescence images measured with a 475 ± 25-nm band pass filter were obtained using a new, high-sensitivity CMOS imager. Ratio images (F365ex/F405ex) were created to evaluate the lesion brightness compared with that of normal mucosa, and specimens were categorized into a no signal or high signal group.


RESULTS
Adenomas and adenocarcinomas were depicted in 87 ratio images, with 86.2% (n = 75) in the High signal group. SSA/P was depicted in 20 ratio images, with 70.0% (n = 14) in the High signal group.


CONCLUSIONS
Dual-wavelength excitation autofluorescence images of colorectal tumours can be acquired using our high-sensitivity CMOS imager, and are useful in detecting colorectal tumours.
Department of Gastroenterology and Metabolism, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan. kominami@hiroshima-u.ac.jp.
Yoko
Y
Kominami
Department of Endoscopy and Medicine, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan. yoshida7@hiroshima-u.ac.jp.
Shigeto
S
Yoshida
Department of Endoscopy and Medicine, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan. colon@hiroshima-u.ac.jp.
Shinji
S
Tanaka
Department of Gastroenterology and Metabolism, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan. miyaki@hosp.city.hiroshima.med.or.jp.
Rie
R
Miyaki
Department of Endoscopy and Medicine, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan. y-sanomura@hiroshima-u.ac.jp.
Yoji
Y
Sanomura
Research Institute of Electronics, Shizuoka University, Shizuoka, Japan. mwseo@idl.rie.shizuoka.ac.jp.
Min-Woong
MW
Seo
Research Institute of Electronics, Shizuoka University, Shizuoka, Japan. kagawa@idl.rie.shizuoka.ac.jp.
Keiichiro
K
Kagawa
Research Institute of Electronics, Shizuoka University, Shizuoka, Japan. kawahito@idl.rie.shizuoka.ac.jp.
Shoji
S
Kawahito
Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan. arimoto-h@aist.go.jp.
Hidenobu
H
Arimoto
Graduate School of Medicine, Division of Health Sciences, Osaka University, Osaka, Japan. k-yamada@sahs.med.osaka-u.ac.jp.
Kenji
K
Yamada
Department of Gastroenterology and Metabolism, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan. chayama@hiroshima-u.ac.jp.
Kazuaki
K
Chayama
D000230:Adenocarcinoma / Q000175:diagnosis*; D000236:Adenoma / Q000175:diagnosis*; D000368:Aged; D003111:Colonic Polyps / Q000175:diagnosis*; D015179:Colorectal Neoplasms / Q000175:diagnosis*; D003430:Cross-Sectional Studies; D005260:Female; D005453:Fluorescence; D006801:Humans; D008297:Male; D008875:Middle Aged; D061848:Optical Imaging / Q000295:instrumentation / Q000379:methods*; D012666:Semiconductors