Biological characterization of liver fatty acid binding gene from miniature pig liver cDNA library.
Liver fatty acid binding proteins (L-FABP) are a family of small, highly conserved, cytoplasmic proteins that bind to long-chain fatty acids and other hydrophobic ligands. In this study, a full-length enriched cDNA library was successfully constructed from Wuzhishan miniature pig, and then the L-FABP gene was cloned from this cDNA library and an expression vector (pEGFP-N3-L-FABP) was constructed in vitro. This vector was transfected into hepatocytes to test its function. The results of western blotting analysis demonstrated that the L-FABP gene from our full-length enriched cDNA library regulated downstream genes, including the peroxisome proliferator-activated receptor family in hepatocytes. This study provides a theoretical basis and experimental evidence for the application of L-FABP for the treatment of liver injury.
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Y H
YH
Gao
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
K F
KF
Wang
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
S
S
Zhang
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Y N
YN
Fan
Laboratório de Genética e Biologia Molecular, Departamento de Biologia, Universidade Federal do Maranhão, São Luís, MA, Brasil weijunguan301@gmail.com.
W J
WJ
Guan
Laboratório de Genética e Biologia Molecular, Departamento de Biologia, Universidade Federal do Maranhão, São Luís, MA, Brasil.
Y H
YH
Ma
D000818:Animals; D003001:Cloning, Molecular; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D015723:Gene Library; D022781:Hepatocytes / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D013552:Swine
D050556:Fatty Acid-Binding Proteins