Immunoregulation on Mice of Low Immunity and Effects on Five Kinds of Human Cancer Cells of Panax japonicus Polysaccharide.
The goal of this study is to investigate the immunoregulative effects of Panax japonicus polysaccharide (PJPS) on mice of low immunity. An orthogonal experiment was designed to determine the best extraction process for PJPS. By the tests of macrophages swallow chicken red blood cells, Delayed-type hypersensitivity (DTH), and serum hemolysin value, we studied the immune adjustment ability of PJPS. MTT was employed to detect the effects of different concentrations of PJPS, respectively, in 24 h, 48 h, and 72 h on five kinds of human cancer cells. The results show that the best extraction process for PJPS was as follows: ratio of solvent consumption to raw material 40, extraction temperature 100°C, re-extracted two times, each extraction time 4 hours. PJPS can significantly improve the immune function of mice processed by cyclophosphamide and PJPS did not work on the above five cancer cells.
Third Xiangya Hospital, Central South University, Changsha 410013, China.
Zhang
Z
Jie
Third Xiangya Hospital, Central South University, Changsha 410013, China ; Changsha University, Changsha 410022, China.
Li
L
Chun-Yan
School of Pharmaceutical Science, Central South University, Changsha 410013, China.
Li
L
Jing-Ping
Third Xiangya Hospital, Central South University, Changsha 410013, China.
Guo
G
Ren
The Central Hospital of Taian, Taian 271000, China.
Wang
W
Hui
Maternal and Child Health Hospital, Liuyang 410300, China.
Pan
P
Juan
Third Xiangya Hospital, Central South University, Changsha 410013, China.
Liu
L
Sheng-Lan