Long non-coding RNA expression profile in atrial fibrillation.
To investigate the expression profiles of long non-coding RNAs (lncRNAs) in atrial fibrillation (AF), atrial tissues from 3 AF patients and 3 non-AF patients that were collected for lncRNA expression microarray analyses to explore the role of lncRNA in the pathogenesis of AF. Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to identify the main functions of the differentially expressed genes and AF-related pathways. A total of 219 lncRNAs was found to be differentially expressed between AFs and controls. Among them, 156 were upregulated and 63 were downregulated. Eight out of 10 dysregulated lncRNAs such as uc001eqh.1 were validated by quantitative real-time PCR. GO categories, pathway analyses, and interaction network showed a consistent result that differentially expressed genes contribute to the pathogenesis of AF. In conclusion, the findings of our study provide a perspective on lncRNA in AF and the foundation for further study of the biological functions of lncRNAs in AF.
Department of Cardiology, Taizhou People's Hospital Taizhou, Jiangsu, China.
Zhongbao
Z
Ruan
Department of Cardiology, Taizhou People's Hospital Taizhou, Jiangsu, China.
Xiaohua
X
Sun
CMC Biobank and Translational Medicine Institute Taizhou, Jiangsu, China ; National Engineering Center for Biochip at Shanghai Shanghai, China ; Taizhou Outdo Clinical Laboratory Taizhou, Jiangsu, China.
Haihui
H
Sheng
Department of Cardiology, Taizhou People's Hospital Taizhou, Jiangsu, China.
Li
L
Zhu
D001281:Atrial Fibrillation / Q000175:diagnosis / Q000235:genetics*; D016022:Case-Control Studies; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D056726:Genetic Association Studies; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
D005819:Genetic Markers; D062085:RNA, Long Noncoding
Long non-coding RNA; atrial fibrillation; gene expression profile; pathway