Discovering New Biology through Sequencing of RNA.
Sequencing of RNA (RNA-Seq) was invented approximately 1 decade ago and has since revolutionized biological research. This update provides a brief historic perspective on the development of RNA-Seq and then focuses on the application of RNA-Seq in qualitative and quantitative analyses of transcriptomes. Particular emphasis is given to aspects of data analysis. Since the wet-lab and data analysis aspects of RNA-Seq are still rapidly evolving and novel applications are continuously reported, a printed review will be rapidly outdated and can only serve to provide some examples and general guidelines for planning and conducting RNA-Seq studies. Hence, selected references to frequently update online resources are given.
Institute of Plant Biochemistry, Cluster of Excellence on Plant Science, Heinrich-Heine-Universität, D-40231 Duesseldorf, Germany andreas.weber@hhu.de.
Andreas P M
http://orcid.org/0000-0003-0970-4672
AP
Weber
D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*; D014158:Transcription, Genetic
D018749:RNA, Plant