Decursin from Angelica gigas Nakai Inhibits B16F10 Melanoma Growth Through Induction of Apoptosis.
Decursin, a bioactive phytochemical isolated from Angelica gigas Nakai (danggwi), has shown preclinical anticancer efficacy in various cancer models. However, the antitumor effect of decursin in melanoma models remains undefined. The antitumor activities of decursin were investigated in B16F10 cells in vitro and in vivo. In this study, we show that treatment with decursin inhibited cell proliferation in a dose-dependent manner in B16F10 cells, but not in normal cells. Decursin also induced apoptosis in B16F10 cells, as determined by annexin V-staining assay and transferase-mediated nick-end labeling (TUNEL) staining assay. Decursin increased the phosphorylation of p38 as well as the expression of Bax while decreasing the phosphorylation of extracellular signaling-regulated kinase (ERK) and the expression of Bcl-2 in B16F10 cells. Moreover, decursin activated caspase-3 in B16F10 cells and xenograft tumor tissue. Together, these findings support further investigations into the potential use of decursin in the treatment of melanoma cells.
1 School of Ecological Environment and Ecotourism, Kyungpook National University , Gyeongbuk, Korea.
Byung Soo
BS
Kim
2 Department of Leisure and Sports, Kyungpook National University , Gyeongbuk, Korea.
Hyobin
H
Seo
3 Department of Physiology, School of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kyungpook National University , Daegu, Korea.
Ha-Jeong
HJ
Kim
4 Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute , School of Medicine, Daegu, Korea.
Sang Mun
SM
Bae
4 Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute , School of Medicine, Daegu, Korea.
Hye-Nam
HN
Son
5 Department of Life Science, Yeungnam University , Gyeongbuk, Korea.
Yoon Jeong
YJ
Lee
2 Department of Leisure and Sports, Kyungpook National University , Gyeongbuk, Korea.
Sungpil
S
Ryu
4 Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute , School of Medicine, Daegu, Korea.
Rang-Woon
RW
Park
1 School of Ecological Environment and Ecotourism, Kyungpook National University , Gyeongbuk, Korea.|6 Department of Food Science and Biotechnology, Kyungpook National University , Gyeongbuk, Korea.
Ju-Ock
JO
Nam
D016475:3T3 Cells; D029969:Angelica / Q000737:chemistry*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic; D017209:Apoptosis / Q000187:drug effects*; D001578:Benzopyrans / Q000494:pharmacology* / Q000627:therapeutic use; D002087:Butyrates / Q000494:pharmacology* / Q000627:therapeutic use; D049109:Cell Proliferation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D008297:Male; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010766:Phosphorylation / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D023041:Xenograft Model Antitumor Assays; D051028:bcl-2-Associated X Protein / Q000032:analysis; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D001578:Benzopyrans; D002087:Butyrates; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C101278:decursin; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases
Bax; Bcl-2; antiproliferation; antitumor; apoptosis