The activation of Epimedium polysaccharide-propolis flavone liposome on Kupffer cells.
Epimedium polysaccharide-propolis flavone liposome (EPL), a potent immunological pharmaceutical preparation, was investigated for the immunomodulatory activity on Kupffer cells (KCs) in vitro. The results showed that EPL could significantly induce the secretion of chemokines (RANTES and MCP-1), promote the production of nitric oxide and induced nitric oxide synthase, improve the pinocytic and phagocytic activity of KCs, promote the mRNA expression of TNF-α and IL-1β, and enhance the expression of costimulatory molecules (CD11b and CD68) in KCs compared with Epimedium polysaccharide-propolis flavone (EP) at 30-7.5μg/mL. In addition, the abilities of KCs on stimulating lymphocytes proliferation and antigen presenting were significantly enhanced after stimulated with EPL compared with EP. These results suggested that EPL could activate KCs and possessed the stronger immunomodulatory effect, which provided the theoretical basis for further studying the mechanism of EPL on improving the immune response.
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Yunpeng
Y
Fan
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Meimei
M
Ren
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Weifeng
W
Hou
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Chao
C
Guo
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: dwtong@nwsuaf.edu.cn.
Dewen
D
Tong
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Lin
L
Ma
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Weimin
W
Zhang
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Mengmeng
M
He
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: sxpxbnl@163.com.
Xiaoping
X
Song
D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018932:Chemokine CCL2 / Q000378:metabolism; D018946:Chemokine CCL5 / Q000378:metabolism; D034181:Epimedium / Q000737:chemistry*; D047309:Flavones / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D007155:Immunologic Factors / Q000737:chemistry* / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000235:genetics; D007728:Kupffer Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008081:Liposomes; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D011429:Propolis / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D039481:CD11b Antigen; D018932:Chemokine CCL2; D018946:Chemokine CCL5; D047309:Flavones; D007155:Immunologic Factors; D053583:Interleukin-1beta; D008081:Liposomes; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D011134:Polysaccharides; D011429:Propolis; D014409:Tumor Necrosis Factor-alpha; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C491768:Ccl2 protein, mouse; C043562:flavone
Epimedium polysaccharide-propolis flavone liposome; Immunomodulatory effect; Kupffer cells