Ameliorating effects of goby fish protein hydrolysates on high-fat-high-fructose diet-induced hyperglycemia, oxidative stress and deterioration of kidney function in rats.
This study investigated the therapeutic potential of undigested goby fish (Zosterisessor ophiocephalus) muscle proteins (UGP) and their hydrolysates on high-fat-high-fructose diet (HFFD)-fed rats. HFFD induced hyperglycemia, manifested by a significant increase in the levels of glucose and glycogen as well as α-amylase activity when compared to normal rats. The administration of GPHs to HFFD-fed rats significantly decreased α-amylase activity and the contents of blood glucose and hepatic glycogen. By contrast, the UGP increased the glucose metabolic disorders in HFFD-fed rats. Furthermore, HFFD-fed rats showed oxidative stress, as evidenced by decreased antioxidant enzyme activities and glutathione (GSH) levels and increased concentration of the lipid peroxidation product malondialdehyde in liver and kidney. Interestingly, the daily gavage of UGP and GPHs improved the redox status in liver and kidney of HFFD-rats by ameliorating or reversing the above-mentioned changes. Moreover, GPHs exhibited a renal protective role by reversing the HFFD-induced decease of uric acid and increase of creatinine levels in serum and preventing some HFFD-induced changes in kidney architecture. The results demonstrate that GPHs contain bioactive peptides that possess significant hypoglycemic and antioxidant properties, and ameliorate renal damage in rats fed hypercaloric diet.
Laboratory of Enzyme Engineering and Microbiology, University of Sfax, National School of Engineering of Sfax (ENIS), P.O. Box 1173, Sfax 3038, Tunisia. Electronic address: ryma47@hotmail.fr.
Rim
R
Nasri
Laboratory of Enzyme Engineering and Microbiology, University of Sfax, National School of Engineering of Sfax (ENIS), P.O. Box 1173, Sfax 3038, Tunisia.
Ola
O
Abdelhedi
Laboratory of Enzyme Engineering and Microbiology, University of Sfax, National School of Engineering of Sfax (ENIS), P.O. Box 1173, Sfax 3038, Tunisia.
Ines
I
Jemil
Laboratory of Animal Ecophysiology, University of Sfax, Faculty of Sciences of Sfax (FSS), P.O. Box 95, Sfax 3052, Tunisia.
Ines
I
Daoued
Laboratory of Animal Ecophysiology, University of Sfax, Faculty of Sciences of Sfax (FSS), P.O. Box 95, Sfax 3052, Tunisia.
Khaled
K
Hamden
Laboratory of Hematology, CHU Habib Bourguiba, Sfax, Tunisia.
Choumous
C
Kallel
Laboratory of Animal Ecophysiology, University of Sfax, Faculty of Sciences of Sfax (FSS), P.O. Box 95, Sfax 3052, Tunisia.
Abdelfattah
A
Elfeki
Laboratoire de Nutrition Clinique et Métabolique, Département de Biologie, Faculté des Sciences, Université d'Oran, B-P 16 ES-Sénia, Oran, Algeria.
Myriem
M
Lamri-Senhadji
Laboratoire de Nutrition Clinique et Métabolique, Département de Biologie, Faculté des Sciences, Université d'Oran, B-P 16 ES-Sénia, Oran, Algeria.
Ahmed
A
Boualga
Laboratory of Enzyme Engineering and Microbiology, University of Sfax, National School of Engineering of Sfax (ENIS), P.O. Box 1173, Sfax 3038, Tunisia.
Moncef
M
Nasri
Laboratory of Enzyme Engineering and Microbiology, University of Sfax, National School of Engineering of Sfax (ENIS), P.O. Box 1173, Sfax 3038, Tunisia.
Maha
M
Karra-Châabouni
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D029941:Fish Proteins / Q000032:analysis / Q000737:chemistry; D005632:Fructose / Q000009:adverse effects; D006943:Hyperglycemia / Q000188:drug therapy* / Q000209:etiology; D007668:Kidney / Q000187:drug effects* / Q000502:physiology; D008099:Liver / Q000187:drug effects / Q000502:physiology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010473:Perciformes; D011492:Protein Hydrolysates / Q000494:pharmacology*; D017208:Rats, Wistar
D000596:Amino Acids; D000975:Antioxidants; D029941:Fish Proteins; D011492:Protein Hydrolysates; D005632:Fructose
Goby fish; Oxidative stress; Postprandial hyperglycemia; Protein hydrolysates; Renoprotective