Dodecenyl succinylated alginate as a novel material for encapsulation and hyperactivation of lipases.
Alginate was modified with dodecenyl succinic anhydride (SAC12) in an aqueous reaction medium at neutral pH. The highest degree of succinylation (33.9±3.5%) was obtained after 4h at 30°C, using four mole SAC12 per mol alginate monomer. Alginate was modified with succinic anhydride (SAC0) for comparison, and the structures and thermal properties of alg-SAC0 and alg-SAC12 were evaluated using FTIR, (1)H NMR, and DSC. Calcium-hydrogel beads were formed from native and modified alginates, in which lipases were encapsulated with a load of averagely 76μg lipase per mg alginate, irrespective of the type of alginate. Lipases with a "lid", which usually are dependent on interfacial activation, showed a 3-fold increase in specific activity toward water-soluble substrates when encapsulated in alg-SAC12, compared to the free lipase. Such hyperactivation was not observed for lipases independent of interfacial activation, or for lipases encapsulated in native alginate or alg-SAC0 hydrogels.
Department of Engineering, Aarhus University, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark; Center for Food Technology, Danish Technological Institute, Kongsvang Alle 29, DK-8000 Aarhus C, Denmark. Electronic address: mia@falkeborg.dk.
Mia
M
Falkeborg
Department of Engineering, Aarhus University, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark; Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Thailand. Electronic address: pattarapon@eng.au.dk.
Pattarapon
P
Paitaid
Department of Engineering, Aarhus University, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark. Electronic address: ndonwiallen@yahoo.com.
Allen Ndonwi
AN
Shu
Department of Engineering, Aarhus University, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark. Electronic address: bperez@eng.au.dk.
Bianca
B
Pérez
Department of Engineering, Aarhus University, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark. Electronic address: guo@eng.au.dk.
Zheng
Z
Guo
D000464:Alginates / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry*; D002214:Capsules; D002626:Chemistry, Pharmaceutical; D004789:Enzyme Activation; D032641:Eurotiales / Q000201:enzymology; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D058105:Polymerization; D013387:Succinic Anhydrides / Q000737:chemistry*; D013696:Temperature
D000464:Alginates; D002214:Capsules; D005656:Fungal Proteins; D006603:Hexuronic Acids; D020100:Hydrogels; D013387:Succinic Anhydrides; C031801:succinic anhydride; D020723:Glucuronic Acid; D008049:Lipase; C431349:lipase B, Candida antarctica
Alginate; Dodecenyl succinylated alginate; Encapsulation; Hyperactivation; Lipase