A Point-of-Care Prothrombin Time Test on a Microfluidic Disk Analyzer Using Alternate Spinning.
In this study, we conducted a fully integrated point-of-care prothrombin time test on a microfluidic disk analyzer. The microfluidic functions integrated on the disk were capable of separating whole blood, decanting plasma, and mixing it with reagents in sequence under alternate spinning. The assay protocol was completed by alternate spinning without using microvalves or surface modification. Clinical sample tests on prothrombin time measurement were conducted by both the microfluidic disk analyzer and the reference instrument used in medical centers. The test results showed a good correlation and agreement between the two instruments.
Chia-Hui
CH
Lin
Kun-Wei
KW
Lin
Daniel
D
Yen
Chih-Hsin
CH
Shih
Chien-Hsing
CH
Lu
Jiunn-Min
JM
Wang
Chi-Yu
CY
Lin
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001781:Blood Component Removal / Q000295:instrumentation*; D002498:Centrifugation / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008297:Male; D008875:Middle Aged; D019095:Point-of-Care Systems; D011517:Prothrombin Time / Q000295:instrumentation*; D015203:Reproducibility of Results; D012399:Rotation; D012680:Sensitivity and Specificity