Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion.
Coherence-controlled holographic microscopy (CCHM) in low-coherence mode possesses a pronounced coherence gate effect. This offers an option to investigate the details of cellular events leading to cell death caused by cytopathic turbid emulsions. CCHM capacity was first assessed in model situations that showed clear images obtained with low coherence of illumination but not with high coherence of illumination. Then, the form of death of human cancer cells induced by treatment with biologically active phospholipids (BAPs) preparation was investigated. The observed overall retraction of cell colony was apparently caused by the release of cell-to-substratum contacts. This was followed by the accumulation of granules decorating the nuclear membrane. Then, the occurrence of nuclear membrane indentations signaled the start of damage to the integrity of the cell nucleus. In the final stage, cells shrunk and disintegrated. This indicated that BAPs cause cell death by necrosis and not apoptosis. An intriguing option of checking the fate of cancer cells caused by the anticipated cooperative effect after adding another tested substance sodium dichloroacetate to turbid emulsion is discussed on grounds of pilot experiments. Such observations should reveal the impact and mechanism of action of the interacting drugs on cell behavior and fate that would otherwise remain hidden in turbid milieu.
Brno University of Technology, CEITEC-Central European Institute of Technology, Technicka 3058/10, Brno 616 00, Czech RepublicbBrno University of Technology, Faculty of Mechanical Engineering, Institute of Physical Engineering, Technicka 2896/2, Brno 616.
Jana
J
Collakova
Brno University of Technology, CEITEC-Central European Institute of Technology, Technicka 3058/10, Brno 616 00, Czech RepublicbBrno University of Technology, Faculty of Mechanical Engineering, Institute of Physical Engineering, Technicka 2896/2, Brno 616.
Aneta
A
Krizova
Brno University of Technology, CEITEC-Central European Institute of Technology, Technicka 3058/10, Brno 616 00, Czech Republic.
Vera
V
Kollarova
Brno University of Technology, CEITEC-Central European Institute of Technology, Technicka 3058/10, Brno 616 00, Czech RepublicbBrno University of Technology, Faculty of Mechanical Engineering, Institute of Physical Engineering, Technicka 2896/2, Brno 616.
Zbynek
Z
Dostal
Brno University of Technology, CEITEC-Central European Institute of Technology, Technicka 3058/10, Brno 616 00, Czech RepublicbBrno University of Technology, Faculty of Mechanical Engineering, Institute of Physical Engineering, Technicka 2896/2, Brno 616.
Michala
M
Slaba
Brno University of Technology, CEITEC-Central European Institute of Technology, Technicka 3058/10, Brno 616 00, Czech Republic.
Pavel
P
Vesely
Brno University of Technology, CEITEC-Central European Institute of Technology, Technicka 3058/10, Brno 616 00, Czech RepublicbBrno University of Technology, Faculty of Mechanical Engineering, Institute of Physical Engineering, Technicka 2896/2, Brno 616.
Radim
R
Chmelik
D016923:Cell Death / Q000502:physiology*; D045744:Cell Line, Tumor; D003584:Cytological Techniques / Q000379:methods*; D003999:Dichloroacetic Acid; D006696:Holography / Q000379:methods*; D006801:Humans; D008853:Microscopy / Q000379:methods*; D009336:Necrosis; D009369:Neoplasms / Q000503:physiopathology*; D010743:Phospholipids
D010743:Phospholipids; D003999:Dichloroacetic Acid