Altered expression of TaRSL4 gene by genome interplay shapes root hair length in allopolyploid wheat.
Polyploidy is a major driving force in plant evolution and speciation. Phenotypic changes often arise with the formation, natural selection and domestication of polyploid plants. However, little is known about the consequence of hybridization and polyploidization on root hair development. Here, we report that root hair length of synthetic and natural allopolyploid wheats is significantly longer than those of their diploid progenitors, whereas no difference is observed between allohexaploid and allotetraploid wheats. The expression of wheat gene TaRSL4, an orthologue of AtRSL4 controlling the root hair development in Arabidopsis, was positively correlated with the root hair length in diploid and allotetraploid wheats. Moreover, transcript abundance of TaRSL4 homoeologue from A genome (TaRSL4-A) was much higher than those of other genomes in natural allopolyploid wheat. Notably, increased root hair length by overexpression of the TaRSL4-A in wheat led to enhanced shoot fresh biomass under nutrient-poor conditions. Our observations indicate that increased root hair length in allohexaploid wheat originated in the allotetraploid progenitors and altered expression of TaRSL4 gene by genome interplay shapes root hair length in allopolyploid wheat.
State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Yuanmingyuan Xi Road No. 2, Haidian District, Beijing, 100193, China.
Yao
Y
Han
State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Yuanmingyuan Xi Road No. 2, Haidian District, Beijing, 100193, China.
Mingming
M
Xin
State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Yuanmingyuan Xi Road No. 2, Haidian District, Beijing, 100193, China.
Ke
K
Huang
State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Yuanmingyuan Xi Road No. 2, Haidian District, Beijing, 100193, China.
Yuyun
Y
Xu
State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Yuanmingyuan Xi Road No. 2, Haidian District, Beijing, 100193, China.
Zhenshan
Z
Liu
State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Yuanmingyuan Xi Road No. 2, Haidian District, Beijing, 100193, China.
Zhaorong
Z
Hu
State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Yuanmingyuan Xi Road No. 2, Haidian District, Beijing, 100193, China.
Yingyin
Y
Yao
State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Yuanmingyuan Xi Road No. 2, Haidian District, Beijing, 100193, China.
Huiru
H
Peng
State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Yuanmingyuan Xi Road No. 2, Haidian District, Beijing, 100193, China.
Zhongfu
Z
Ni
State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Yuanmingyuan Xi Road No. 2, Haidian District, Beijing, 100193, China.
Qixin
Q
Sun
D029681:Arabidopsis Proteins / Q000235:genetics; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D018533:Biomass; D004171:Diploidy; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D018517:Plant Roots / Q000033:anatomy & histology / Q000235:genetics*; D018520:Plant Shoots / Q000235:genetics; D030821:Plants, Genetically Modified; D011123:Polyploidy; D014908:Triticum / Q000235:genetics*
D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; C548371:RSL4 protein, Arabidopsis
TaRSL4; gene expression; homoeologue; polyploid wheat; root hair length