Design of an optically stable pH sensor based on immobilization of Giemsa on triacetylcellulose membrane.
In this work a simple, inexpensive, and sensitive optical sensor based on triacetylcellulose membrane as solid support was developed by using immobilization of Giemsa indicator for pH measurement. In this method, the influence variables on the membrane performance including pH concentration of indicator, response time, ionic strength, and reversibility were investigated. At optimum values of all variables the response of optical pH sensor is linear in the pH range of 3.0-12.0. This optical sensor was produced through simultaneous binding of the Giemsa on the activated triacetylcellulose membrane which responded to the pH changes in a broader linear range within less than 2.0 min and suitable reproducibility (RSD<5%). Stability results showed that this sensor was stable after 6 months of storage in the water/ethanol (50:50, v/v) solution without any measurable divergence in response properties (less than 5% RSD).
Department of Chemistry, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.
Saeid
S
Khodadoust
Abadan School of Medical Sciences, Abadan, Iran.
Narges Cham
NC
Kouri
Medicinal Plants Research Center, Yasuj University of Medical Science, Yasuj, Iran. Electronic address: taleb_84@yahoo.com.
Mohammad Sharif
MS
Talebiyanpoor
Abadan School of Medical Sciences, Abadan, Iran.
Jamile
J
Deris
Behbahan Faculty of Medical Sciences, Behbahan, Iran.
Arezou Amiri
AA
Pebdani
D000327:Adsorption; D001399:Azure Stains / Q000737:chemistry*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D055096:Optical Devices; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation*
D001399:Azure Stains; D008567:Membranes, Artificial; D002482:Cellulose; C024671:cellulose triacetate
Giemsa; Triacetylcellulose membrane; pH optical sensor