Using a Personal Glucose Meter and Alkaline Phosphatase for Point-of-Care Quantification of Galactose-1-Phosphate Uridyltransferase in Clinical Galactosemia Diagnosis.
The personal glucose meter (PGM) was recently shown to be a general meter to detect many targets. Most studies, however, focus on transforming either target binding or enzymatic activity that cleaves an artificial substrate into the production of glucose. More importantly, almost all reports exhibit their methods by using artificial samples, such as buffers or serum samples spiked with the targets. To expand the technology to even broader targets and to validate its potential in authentic, more complex clinical samples, we herein report expansion of the PGM method by using alkaline phosphatase (ALP) that links the enzymatic activity of galactose-1-phosphate uridyltransferase to the production of glucose, which allows point-of-care galactosemia diagnosis in authentic human clinical samples. Given the presence of ALP in numerous enzymatic assays for clinical diagnostics, the methods demonstrated herein advance the field closer to point-of-care detection of a wide range of targets in real clinical samples.
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Jingjing
J
Zhang
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Yu
Y
Xiang
Division of Genetics, University of Illinois at Chicago, 840 S Wood St, CSB, Chicago, IL, 60612, USA.
Donna E
DE
Novak
Division of Genetics, University of Illinois at Chicago, 840 S Wood St, CSB, Chicago, IL, 60612, USA.
George E
GE
Hoganson
School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China.
Junjie
J
Zhu
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. yi-lu@illinois.edu.
Yi
Y
Lu
D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation*; D002236:Carbohydrate Conformation; D002417:Cattle; D004912:Erythrocytes / Q000737:chemistry; D005693:Galactosemias / Q000175:diagnosis*; D006736:Horses / Q000097:blood; D006801:Humans; D019095:Point-of-Care Systems; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase / Q000378:metabolism*
D005694:UTP-Hexose-1-Phosphate Uridylyltransferase; D000469:Alkaline Phosphatase
alkaline phosphatase; biosensors; carbohydrates; galactosemia; personal glucose meter