Integrated Proteome Analysis of the Wheat Embryo and Endosperm Reveals Central Metabolic Changes Involved in the Water Deficit Response during Grain Development.
The embryo and endosperm of wheat have different physiological functions and large differences in protein level. In this study, we performed the first integrated proteome analysis of wheat embryo and endosperm in response to the water deficit during grain development. In total, 155 and 130 differentially expressed protein (DEP) spots in the embryo and endosperm, respectively, were identified by nonlinear two-dimensional electrophoresis and tandem mass spectrometry. These DEPs in the embryo were mainly involved in stress/defense responses such as heat shock-related proteins (HSP) and peroxidase, whereas those in endosperm were mainly related to starch and storage protein synthesis such as α-amylase inhibitor and the globulin-1 S allele. In particular, some storage proteins such as avenin-like proteins and high-molecular weight glutenin subunit Dy12 displayed higher expression levels in the mature endosperm under a water deficit, which might contribute to the improvement in the quality of breadmaking.
College of Life Sciences, Capital Normal University , Beijing 100048, China.
Aiqin
A
Gu
College of Life Sciences, Capital Normal University , Beijing 100048, China.
Pengchao
P
Hao
College of Life Sciences, Capital Normal University , Beijing 100048, China.
Dongwen
D
Lv
College of Life Sciences, Capital Normal University , Beijing 100048, China.
Shoumin
S
Zhen
College of Life Sciences, Capital Normal University , Beijing 100048, China.
Yanwei
Y
Bian
College of Life Sciences, Capital Normal University , Beijing 100048, China.
Chaoying
C
Ma
Hubei Collaborative Innovation Center for Grain Industry, Yangtze University , 434025 Jingzhou, China.
Yanhao
Y
Xu
Hubei Collaborative Innovation Center for Grain Industry, Yangtze University , 434025 Jingzhou, China.
Wenying
W
Zhang
College of Life Sciences, Capital Normal University , Beijing 100048, China.|Hubei Collaborative Innovation Center for Grain Industry, Yangtze University , 434025 Jingzhou, China.
Yueming
Y
Yan
D015180:Electrophoresis, Gel, Two-Dimensional; D056625:Endosperm / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D013058:Mass Spectrometry; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D014908:Triticum / Q000737:chemistry / Q000196:embryology / Q000235:genetics / Q000378:metabolism*
D010940:Plant Proteins; D020543:Proteome
embryo; endosperm; proteome; water deficit; wheat