Vba4p, a vacuolar membrane protein, is involved in the drug resistance and vacuolar morphology of Saccharomyces cerevisiae.
In the vacuolar basic amino acid (VBA) transporter family of Saccharomyces cerevisiae, VBA4 encodes a vacuolar membrane protein with 14 putative transmembrane helices. Transport experiments with isolated vacuolar membrane vesicles and estimation of the amino acid contents in vacuoles showed that Vba4p is not likely involved in the transport of amino acids. We found that the vba4Δ cells, as well as vba1Δ and vba2Δ cells, showed increased susceptibility to several drugs, particularly to azoles. Although disruption of the VBA4 gene did not affect the salt tolerance of the cells, vacuolar fragmentation observed under high salt conditions was less prominent in vba4Δ cells than in wild type, vba1Δ, and vba2Δ cells. Vba4p differs from Vba1p and Vba2p as a vacuolar transporter but is important for the drug resistance and vacuolar morphology of S. cerevisiae.
a Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture , Ehime University , Matsuyama , Japan.|b Advanced Research Support Center (ADRES) , Ehime University , Matsuyama , Japan.
Miyuki
M
Kawano-Kawada
a Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture , Ehime University , Matsuyama , Japan.
Pongsanat
P
Pongcharoen
a Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture , Ehime University , Matsuyama , Japan.
Rieko
R
Kawahara
a Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture , Ehime University , Matsuyama , Japan.
Mayu
M
Yasuda
a Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture , Ehime University , Matsuyama , Japan.
Takashi
T
Yamasaki
a Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture , Ehime University , Matsuyama , Japan.|b Advanced Research Support Center (ADRES) , Ehime University , Matsuyama , Japan.
Koichi
K
Akiyama
a Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture , Ehime University , Matsuyama , Japan.
Takayuki
T
Sekito
a Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture , Ehime University , Matsuyama , Japan.
Yoshimi
Y
Kakinuma
D026923:Amino Acid Transport Systems, Basic / Q000235:genetics / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D000935:Antifungal Agents / Q000494:pharmacology*; D001692:Biological Transport; D025141:Drug Resistance, Fungal / Q000235:genetics*; D015725:Fluconazole / Q000494:pharmacology; D015870:Gene Expression; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007654:Ketoconazole / Q000494:pharmacology; D008825:Miconazole / Q000494:pharmacology; D053140:Organelle Shape; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D055049:Salt Tolerance; D012965:Sodium Chloride / Q000494:pharmacology; D014617:Vacuoles / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D026923:Amino Acid Transport Systems, Basic; D000596:Amino Acids; D000935:Antifungal Agents; D020033:Protein Isoforms; D012965:Sodium Chloride; D008825:Miconazole; D015725:Fluconazole; D007654:Ketoconazole
azole; multidrug resistance; vacuolar transporter