Expression Pattern of Fatty Acid Binding Proteins in Celiac Disease Enteropathy.
Celiac disease (CD) is an immune-mediated enteropathy that develops in genetically susceptible individuals following exposure to dietary gluten. Severe changes at the intestinal mucosa observed in untreated CD patients are linked to changes in the level and in the pattern of expression of different genes. Fully differentiated epithelial cells express two isoforms of fatty acid binding proteins (FABPs): intestinal and liver, IFABP and LFABP, respectively. These proteins bind and transport long chain fatty acids and also have other important biological roles in signaling pathways, particularly those related to PPARγ and inflammatory processes. Herein, we analyze the serum levels of IFABP and characterize the expression of both FABPs at protein and mRNA level in small intestinal mucosa in severe enteropathy and normal tissue. As a result, we observed higher levels of circulating IFABP in untreated CD patients compared with controls and patients on gluten-free diet. In duodenal mucosa a differential FABPs expression pattern was observed with a reduction in mRNA levels compared to controls explained by the epithelium loss in severe enteropathy. In conclusion, we report changes in FABPs' expression pattern in severe enteropathy. Consequently, there might be alterations in lipid metabolism and the inflammatory process in the small intestinal mucosa.
Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT CONICET, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, 1900 La Plata, Argentina.
Natalia M
NM
Bottasso Arias
Departamento de Ciencias Biológicas, Instituto de Estudios Inmunológicos y Fisiopatológicos (IIFP), UNLP-CONICET, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 1900 La Plata, Argentina ; Laboratorio de Inmunología de Enfermedades Respiratorias, Instituto de Medicina Experimental (IMEX), CONICET, Academia Nacional de Medicina, C1425AUM Buenos Aires, Argentina.
Marina
M
García
Departamento de Ciencias Biológicas, Instituto de Estudios Inmunológicos y Fisiopatológicos (IIFP), UNLP-CONICET, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 1900 La Plata, Argentina.
Constanza
C
Bondar
Servicio de Gastroenterología, Hospital de Niños "Sor María Ludovica", 1900 La Plata, Argentina.
Luciana
L
Guzman
Servicio de Gastroenterología, Hospital "San Martín", 1900 La Plata, Argentina.
Agustina
A
Redondo
Servicio de Gastroenterología, Hospital "San Martín", 1900 La Plata, Argentina.
Nestor
N
Chopita
Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT CONICET, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, 1900 La Plata, Argentina.
Betina
B
Córsico
Departamento de Ciencias Biológicas, Instituto de Estudios Inmunológicos y Fisiopatológicos (IIFP), UNLP-CONICET, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 1900 La Plata, Argentina.
Fernando G
FG
Chirdo
D002446:Celiac Disease / Q000097:blood*; D004386:Duodenum / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000097:blood* / Q000235:genetics; D006801:Humans; D007421:Intestine, Small / Q000378:metabolism; D047495:PPAR gamma / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
D050556:Fatty Acid-Binding Proteins; D047495:PPAR gamma; D012333:RNA, Messenger