Determination of Rivaroxaban in Human Plasma by Solid-Phase Extraction-High Performance Liquid Chromatography.
In this study, a solid-phase extraction (SPE)-high performance liquid chromatography (HPLC)-ultra violet (UV) method was developed for the determination of rivaroxaban (RIV), an oral anticoagulant drug, in human plasma samples. The concentration of RIV in plasma samples was increased 7.5 times and the interference coming from matrix components was avoided by using SPE. The extracted samples of RIV were analyzed by using an HPLC-UV method. RIV was approved in 2008 and many studies have been published in recent years in order to investigate its pharmacokinetic profile in various groups. In light of this information, it is clear that the RIV pharmacokinetic profile should be investigated in further studies; the HPLC-UV method presented in this study might be an easy method to apply, as it is a cheap and rapid alternative to HPLC-MS-MS for this purpose. A Phenomenex Luna 5-µm C18 100 Å LC column (250 × 4.6 mm) was used for the separation of RIV and prednisolone (internal standard). The total analysis time was <6 min. The method was validated according to the FDA guidelines and can be proposed for pharmacokinetic studies of RIV.
Faculty of Pharmacy, Department of Analytical Chemistry, Hacettepe University, 06100 Ankara, Turkey celebier@hacettepe.edu.tr.
Mustafa
M
Çelebier
Faculty of Pharmacy, Department of Analytical Chemistry, Hacettepe University, 06100 Ankara, Turkey.
Tuba
T
Reçber
Faculty of Pharmacy, Department of Analytical Chemistry, Hacettepe University, 06100 Ankara, Turkey.
Engin
E
Koçak
Faculty of Pharmacy, Department of Analytical Chemistry, Hacettepe University, 06100 Ankara, Turkey.
Sacide
S
Altınöz
Faculty of Pharmacy, Department of Analytical Chemistry, Hacettepe University, 06100 Ankara, Turkey.
Sedef
S
Kır
D000925:Anticoagulants / Q000097:blood* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D000069552:Rivaroxaban / Q000097:blood* / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*
D000925:Anticoagulants; D000069552:Rivaroxaban