Development and Characterization of a New Antipeptide Monoclonal Antibody Directed to Human CD20 Antigen.
The rapid expansion of immunotherapeutic approaches for treatment of various diseases, including cancers, has been greatly facilitated by the invention of new generation of antibodies. Clinical studies have indicated that anti-CD20 mAb-based therapies represent an effective treatment for various diseases with overexpression of CD20 on their cell surface, such as non-Hodgkin's lymphoma, hemolytic anemia, as well as autoimmune diseases like rheumatoid arthritis. Technically, due to a short extra membrane domain, the recombinant CD20 protein is a difficult antigen to raise immune responses. In search for new monoclonal antibodies, the authors used an antigenic polypeptide, which yielded numbers of new binders that may lead to production of anti-CD20 antibodies, with improved diagnostic or clinical attributes. Mice were immunized with extra membrane loop of human CD20 (exCD20) polypeptide. The exCD20 antigen showed a desired immune response and was able to develop a monoclonal antibody, 3B4C10, which reacted well with peptide antigen as well as native antigen on the surface of Raji B-cell line. The antibody 3B4C10 with a balanced K(on) and K(off) may be applicable in the construction of affinity columns or beads for isolation and purification of CD20-positive cells and cancer stem cells.
1 National Cell Bank of Iran, Pasteur Institute of Iran . Tehran, Iran .
Mahdi
M
Habibi-Anbouhi
2 Department of Virology, Pasteur Institute of Iran , Tehran, Iran .
Kayhan
K
Azadmanesh
3 Venom & Biotherapeutics Molecules Laboratory, Biotechnology Research Center, Pasteur Institute of Iran , Tehran, Iran .
Mahdi
M
Behdani
4 Department of Regenerative Medicine at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology , ACECR, Tehran, Iran .
Ensiyeh
E
Hajizadeh-Saffar
5 Department of Hepatitis and AIDS, Pasteur Institute of Iran , Tehran, Iran .
Rouhollah
R
Vahabpour
1 National Cell Bank of Iran, Pasteur Institute of Iran . Tehran, Iran .
Mohammad Ali
MA
Shokrgozar
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D018951:Antigens, CD20 / Q000276:immunology*; D005260:Female; D006801:Humans; D006825:Hybridomas; D019169:Jurkat Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000276:immunology / Q000628:therapy; D010455:Peptides / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018951:Antigens, CD20; D010455:Peptides
CD20; antipeptide antibody; exCD20; peptide antigen