Genetic diversity and silencing suppression activity of the p22 protein of Tomato chlorosis virus isolates from tomato and sweet pepper.
As for other bipartite criniviruses (genus Crinivirus, family Closteroviridae), the genome of Tomato chlorosis virus encodes an RNA silencing suppressor, the protein p22, in the 3'-proximal region of RNA1. This protein has been reported as having one of the longest lasting local suppressor activities when transiently expressed in Nicotiana benthamiana. Here, we examined the genetic diversity of the p22 gene in ToCV isolates from tomato and sweet pepper. The p22 gene sequences clearly grouped into two separated clades. However, functional analysis of both types of p22 proteins indicated no evident differences in suppressor activity. Our findings provide experimental evidence that the presence of a "strong" silencing suppressor is a conserved feature of ToCV isolates.
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora"- Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental "La Mayora", Algarrobo-Costa, 29750, Málaga, Spain.
Yazmín M
YM
Landeo-Ríos
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora"- Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental "La Mayora", Algarrobo-Costa, 29750, Málaga, Spain.
Jesús
J
Navas-Castillo
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora"- Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental "La Mayora", Algarrobo-Costa, 29750, Málaga, Spain.
Enrique
E
Moriones
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora"- Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental "La Mayora", Algarrobo-Costa, 29750, Málaga, Spain. carmen.canizares@eelm.csic.es.
M Carmen
MC
Cañizares
D000595:Amino Acid Sequence; D002212:Capsicum / Q000821:virology; D016000:Cluster Analysis; D029383:Crinivirus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D020868:Gene Silencing; D014644:Genetic Variation; D018551:Solanum lycopersicum / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*
D012367:RNA, Viral; D014764:Viral Proteins
Closteroviridae; Crinivirus; Plant viral suppressor