Developmental expression and function analysis of protein tyrosine phosphatase receptor type D in oligodendrocyte myelination.
Receptor protein tyrosine phosphatases (RPTPs) are extensively expressed in the central nervous system (CNS), and have distinct spatial and temporal patterns in different cell types during development. Previous studies have demonstrated possible roles for RPTPs in axon outgrowth, guidance, and synaptogenesis. In the present study, our results revealed that protein tyrosine phosphatase, receptor type D (PTPRD) was initially expressed in mature neurons in embryonic CNS, and later in oligodendroglial cells at postnatal stages when oligodendrocytes undergo active axonal myelination process. In PTPRD mutants, oligodendrocyte differentiation was normal and a transient myelination delay occurred at early postnatal stages, indicating the contribution of PTPRD to the initiation of axonal myelination. Our results also showed that the remyelination process was not affected in the absence of PTPRD function after a cuprizone-induced demyelination in adult animals.
Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY 40292, USA.
Q
Q
Zhu
Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY 40292, USA; Institute of Developmental and Regenerative Biology, Zhejiang Key Lab of Organ Development and Regeneration, College of Life Sciences, Hangzhou Normal University, China.
Z
Z
Tan
Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY 40292, USA.
S
S
Zhao
Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY 40292, USA; Institute of Developmental and Regenerative Biology, Zhejiang Key Lab of Organ Development and Regeneration, College of Life Sciences, Hangzhou Normal University, China.
H
H
Huang
Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY 40292, USA; Institute of Developmental and Regenerative Biology, Zhejiang Key Lab of Organ Development and Regeneration, College of Life Sciences, Hangzhou Normal University, China.
X
X
Zhao
Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY 40292, USA.
X
X
Hu
Norton Neuroscience Institute, Norton Healthcare, Louisville, KY 40202, USA.
Y
Y
Zhang
Norton Neuroscience Institute, Norton Healthcare, Louisville, KY 40202, USA.
C B
CB
Shields
McGill Cancer Centre and Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada.
N
N
Uetani
Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY 40292, USA; Institute of Developmental and Regenerative Biology, Zhejiang Key Lab of Organ Development and Regeneration, College of Life Sciences, Hangzhou Normal University, China. Electronic address: m0qiu001@louisville.edu.
M
M
Qiu
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000254:growth & development* / Q000473:pathology / Q000502:physiology* / Q000503:physiopathology; D002454:Cell Differentiation / Q000502:physiology; D003471:Cuprizone; D003711:Demyelinating Diseases / Q000473:pathology / Q000503:physiopathology; D004195:Disease Models, Animal; D019054:Evoked Potentials, Motor / Q000502:physiology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008822:Mice, Transgenic; D009043:Motor Activity / Q000502:physiology; D009154:Mutation; D009836:Oligodendroglia / Q000473:pathology / Q000502:physiology* / Q000648:ultrastructure; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2 / Q000235:genetics / Q000378:metabolism*; D013116:Spinal Cord / Q000254:growth & development* / Q000473:pathology / Q000502:physiology* / Q000503:physiopathology; D050781:Transcranial Magnetic Stimulation
D003471:Cuprizone; C490395:Ptprd protein, mouse; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2
axonal myelination; corpus callosum; cuprizone; spinal cord