Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG'::lux.
A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results.
Department of Chemistry and Bioengineering, Tampere University of Technology, Korkeakoulunkatu 8, FI-33101 Tampere, Finland; Natural Resources Institute Finland (Luke), Parkano Research Unit, Kaironiementie 15, FI-39700 Parkano, Finland. Electronic address: jenni.tienaho@tut.fi.
Jenni
J
Tienaho
Natural Resources Institute Finland (Luke), Parkano Research Unit, Kaironiementie 15, FI-39700 Parkano, Finland. Electronic address: tytti.sarjala@luke.fi.
Tytti
T
Sarjala
Department of Chemistry and Bioengineering, Tampere University of Technology, Korkeakoulunkatu 8, FI-33101 Tampere, Finland. Electronic address: robert.franzen@tut.fi.
Robert
R
Franzén
Department of Chemistry and Bioengineering, Tampere University of Technology, Korkeakoulunkatu 8, FI-33101 Tampere, Finland. Electronic address: matti.karp@tut.fi.
Matti
M
Karp
D000975:Antioxidants / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D057166:High-Throughput Screening Assays / Q000379:methods*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results
D000975:Antioxidants; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D002374:Catalase; C051253:katG protein, E coli; D008156:Luciferases
Antioxidative activity; Bacterial biosensor; Bioscreening; Microplate technique