Phosphorylation at serine 52 and 635 does not alter the transport properties of glucosinolate transporter AtGTR1.
Little is known about how plants regulate transporters of defense compounds. In A. thaliana, glucosinolates are transported between tissues by NPF2.10 (AtGTR1) and NPF2.11 (AtGTR2). Mining of the PhosPhat4.0 database showed two cytosol exposed phosphorylation sites for AtGTR1 and one membrane-buried phosphorylation site for AtGTR2. In this study, we investigate whether mutation of the two potential regulatory sites of AtGTR1 affected transport of glucosinolates in Xenopus oocytes. Characterization of AtGTR1 phosphorylation mutants showed that phosphorylation of AtGTR1 - at the two reported phosphorylation sites - is not directly involved in regulating AtGTR1 transport activity. We hypothesize a role for AtGTR1-phosphorylation in regulating protein-protein interactions.
a Center for Dynamic Molecular Interactions (DynaMo); University of Copenhagen ; Frederiksberg , Denmark.|b Department of Plant and Environmental Sciences ; Faculty of Science; University of Copenhagen ; Frederiksberg , Denmark.
Morten Egevang
ME
Jørgensen
b Department of Plant and Environmental Sciences ; Faculty of Science; University of Copenhagen ; Frederiksberg , Denmark.
Carl Erik
CE
Olsen
a Center for Dynamic Molecular Interactions (DynaMo); University of Copenhagen ; Frederiksberg , Denmark.|b Department of Plant and Environmental Sciences ; Faculty of Science; University of Copenhagen ; Frederiksberg , Denmark.
Barbara Ann
BA
Halkier
a Center for Dynamic Molecular Interactions (DynaMo); University of Copenhagen ; Frederiksberg , Denmark.|b Department of Plant and Environmental Sciences ; Faculty of Science; University of Copenhagen ; Frederiksberg , Denmark.
Hussam Hassan
HH
Nour-Eldin
D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism
D029681:Arabidopsis Proteins; C575185:GTR1 protein, Arabidopsis; D009004:Monosaccharide Transport Proteins; D012694:Serine
NPF2.10 and NPF2.11; POTs; Xenopus laevis; glucosinolate transport; membrane transport; phosphorylation mimicking and transporter regulation; secondary active transport; transport phosphorylation