Bacillus amyloliquefaciens levansucrase-catalyzed the synthesis of fructooligosaccharides, oligolevan and levan in maple syrup-based reaction systems.
Maple syrups with selected degree Brix (°Bx) (15, 30, 60) were investigated as reaction systems for levansucrase from Bacillus amyloliquefaciens. The enzymatic conversion of sucrose present in the maple syrup and the production of the transfructosylation products were assessed over a time course of 48h. At 30°C, the use of maple syrup 30°Bx led to the highest levansucrase activity (427.53μmol/mg protein/min), while maple syrup 66°Bx led to the highest converted sucrose concentration (1.53M). In maple syrup 30°Bx, oligolevans (10<DP≤30) were synthesized as major products (>80%). In maple syrup 66°Bx, the most abundant products were oligolevans at 30°C and levans (DP≥30) at 8°C. The acceptor specificity study revealed the ability of B. amyloliquefaciens levansucrase to synthesize a variety of hetero-fructooligosaccharides (FOSs) in maple syrups 15°Bx and 30°Bx enriched with various disaccharides, with lactose being the preferred fructosyl acceptor. The current study is the first to investigate maple-syrup-based reaction systems for the synthesis of FOSs/oligolevans/levans.
Department of Food Science and Agricultural Chemistry, McGill University, 21,111 Lakeshore Ste Anne de Bellevue, Quebec, QC, Canada H9X 3V9.
Mengxi
M
Li
Department of Food Science and Agricultural Chemistry, McGill University, 21,111 Lakeshore Ste Anne de Bellevue, Quebec, QC, Canada H9X 3V9.
Sooyoun
S
Seo
Department of Food Science and Agricultural Chemistry, McGill University, 21,111 Lakeshore Ste Anne de Bellevue, Quebec, QC, Canada H9X 3V9. Electronic address: salwa.karboune@mcgill.ca.
Salwa
S
Karboune
D031002:Acer / Q000737:chemistry*; D001407:Bacillus / Q000201:enzymology*; D055162:Biocatalysis; D060326:Chemistry Techniques, Synthetic; D005630:Fructans / Q000138:chemical synthesis* / Q000737:chemistry; D005632:Fructose / Q000737:chemistry*; D006602:Hexosyltransferases / Q000378:metabolism*; D007700:Kinetics; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D013379:Substrate Specificity
D005630:Fructans; D009844:Oligosaccharides; D005632:Fructose; C072599:levan; D006602:Hexosyltransferases; C021513:levansucrase
Acceptor specificity; Fructooligosaccharides; Levan; Levansucrase; Maple syrup; Oligolevan