Endodontic drug delivery for root surface disinfection: a laboratory feasibility evaluation.
OBJECTIVE
This study aims to assess the potential of a mixture of three antibiotics (TreVitaMix, TVM) as an intracanal dressing to disinfect the outer root surface by applying a new in vitro model.


METHODS
Fifty freshly extracted bovine roots were endodontically treated. Forty samples were then thoroughly scaled, mounted to petri dishes, gas sterilized, and randomly allocated to four groups (n = 10/group) according to their intracanal medication: sterile saline (NaCl; control, A); the TVM carrier material alone, i.e., propylene glycol (PG; B); TVM (C); and calcium hydroxide (D). In an additional group (E), the cementum was not removed and TVM was placed. Petri dishes were filled with Fastidious Anaerobe Agar, inoculated with Fusobacterium nucleatum suspension and then anaerobically incubated during 48-h intervals at 37 °C up to 192 h. Inhibition zones around the roots were then measured after each incubation period (mm(2)).


RESULTS
Only teeth inoculated with the TVM dressing showed inhibition at all time points, whereas the other treatments showed no peri-radicular growing inhibition. Presence of cementum had no negative effect on disinfection (p = 0.9320).


CONCLUSIONS
TVM was able to penetrate through the dentine and inhibit the bacterial growth of F. nucleatum up to 192 h.


CONCLUSIONS
TVM might have the potential to sustainably disinfect the outer root surface in perio-endo lesions and serve as an adjunctive antimicrobial agent.
Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland. markuszaruba@gmx.de.
Markus
M
Zaruba
Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
Dan-K
DK
Rechenberg
Institute of Oral Biology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
Thomas
T
Thurnheer
Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
Thomas
T
Attin
Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland. patrick.schmidlin@zzm.uzh.ch.
Patrick R
PR
Schmidlin
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002417:Cattle; D002444:Cefuroxime / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology*; D004203:Disinfection; D004338:Drug Combinations; D016967:Fusobacterium nucleatum / Q000187:drug effects*; D066298:In Vitro Techniques; D008795:Metronidazole / Q000494:pharmacology*; D012388:Root Canal Irrigants / Q000494:pharmacology; D014092:Tooth Root / Q000187:drug effects* / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D004338:Drug Combinations; D012388:Root Canal Irrigants; C000621569:TreVitaMix; D008795:Metronidazole; D002939:Ciprofloxacin; D002444:Cefuroxime
Agar diffusion; Bacteria; Dentine; In vitro; Perio-endo lesion; TreVitaMix