Response of carbon assimilation and chlorophyll fluorescence to soybean leaf phosphorus across CO2: Alternative electron sink, nutrient efficiency and critical concentration.
To evaluate the response of CO2 assimilation rate (PN) and various chlorophyll fluorescence (CF) parameters to phosphorus (P) nutrition, soybean plants were grown in controlled environment with sufficient (0.50mM) and deficient (0.10 and 0.01 mM) phosphate (P) supply under ambient and elevated CO2 (aCO2, 400 and eCO2, 800 μmol mol(-1), respectively). Measurements were made at ambient (21%) and low (2%) O2 concentrations. Results showed strong correlation of leaf P concentration with PN and CF parameters. The P deficiency showed parallel decreases in PN, and CF parameters including quantum efficiency (Fv'/Fm'), quantum yield of photosystem II (ΦPSII), electron transport rate (JF), and photochemical quenching (qP). The Fv'/Fm' decreased as a result of greater decline in maximal (Fm') than minimal (Fo') fluorescence. The eCO2 stimulated PN especially under higher leaf P concentrations. Low O2 also stimulated PN but only at aCO2. The photosynthetic carbon reduction (PCR, signified by PN) and photorespiratory carbon oxidation cycles (PCO, signified photorespiration as indicated by ratio of JF to gross PN and % increase in PN at 2% O2) was the major electron sinks. However, the presence of alternative electron sink was also evident as determined by the difference between the electron transport calculated from chlorophyll fluorescence and gas exchange measurements. Alternative electron sink declined at lower leaf P concentration suggesting its minor role in photochemical energy consumption, thus dissipation of the excess excitation pressure of PSII reaction center under P deficiency. The JF/PG and % increase in PN at 2 versus 21% O2 remained consistent across leaf P concentration suggesting PCO cycle as an important mechanism to dissipate excess excitation energy in P deficient leaves. The severe decline of Fv'/Fm', ΦPSII, JF and qP under P deficiency also suggested the occurrences of excess radiant energy dissipation by non-photochemical quenching mechanisms. Critical leaf P concentration (CLPC) needed to achieve 90% of the maximum value was greater for PN than CF parameters. Moreover, CLPC was always higher at eCO2 suggesting increased sensitivity of soybean to P deficiency under eCO2. An increased phosphorus utilization efficiency of PN and CF parameters was also achieved but with the expense of net CO2 assimilation in P-deficient leaves.
Crop Systems and Global Change Laboratory, USDA-ARS, Beltsville, MD 20705, USA; Wye Research and Education Center, University of Maryland, MD, USA. Electronic address: shardendu.singh@ars.usda.gov.
Shardendu K
SK
Singh
Crop Systems and Global Change Laboratory, USDA-ARS, Beltsville, MD 20705, USA.
Vangimalla R
VR
Reddy
D002244:Carbon / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism*; D004583:Electrons; D005453:Fluorescence; D010100:Oxygen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D010788:Photosynthesis; D018515:Plant Leaves / Q000378:metabolism*; D013025:Glycine max / Q000378:metabolism*
D002734:Chlorophyll; D002245:Carbon Dioxide; D010758:Phosphorus; D002244:Carbon; D010100:Oxygen
Carotenoids; Chlorophyll; Photorespiration; Photosynthesis; Quantum yield