Complete mitochondrial genome reveals genetic diversity of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae).
Angiostrongylus cantonensis is a zoonotic parasite that causes eosinophilic meningitis in humans. Earlier work on its mitochondrial genome was based on long polymerase chain reaction method. To date, only the mitogenome of the isolates from China has been studied. We report here the complete mitogenome of the Thailand isolate based on next generation sequencing and compare the genetic diversity with other isolates. The mitogenome of the Thailand isolate (13,519bp) is longer than those of the China isolates (13,497-13,502bp). Five protein-coding genes (atp6, cox1, cox2, cob, nad2) show variations in length among the isolates. The stop codon of the Thailand isolate differs from the China and Taiwan isolates in 4 genes (atp6, cob, nad2, nad6). Additionally, the Thailand isolate has 4 incomplete T stop codon compared to 3 in the China and Taiwan isolates. The control region is longer in the Thailand isolate (258bp) than the China (230-236bp) and Taiwan (237bp) isolates. The intergenic sequence between nad4 and cox1 genes in the Thailand isolate lacks 2bp (indels) at the 5'-end of the sequence as well as differs at 7 other sites compared to the China and Taiwan isolates. In the Thailand isolate, 18 tRNAs lack the entire TΨC-arm, compared to 17 in the China isolate and 16 in the Taiwan isolate. Phylogenetic analyses based on 36 mt-genes, 12 PCGs, 2 rRNA genes, 22 tRNA genes and control region all indicate closer genetic affinity between the China and Taiwan isolates compared to the Thailand isolate. Based on 36 mt-genes, the inter-isolate genetic distance varies from p=3.2% between China and Taiwan isolates to p=11.6% between Thailand and China isolates. The mitogenome will be useful for population, phylogenetics and phylogeography studies.
Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia; Chancellory High Impact Research, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Hoi-Sen
HS
Yong
Chancellory High Impact Research, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Sze-Looi
SL
Song
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. Electronic address: praphathip.eam@mahidol.ac.th.
Praphathip
P
Eamsobhana
Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia; Chancellory High Impact Research, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Share-Yuan
SY
Goh
Institute of Ocean and Earth Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Phaik-Eem
PE
Lim
D017185:Angiostrongylus cantonensis / Q000145:classification / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D014644:Genetic Variation; D054629:Genome, Mitochondrial; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics
D012335:RNA, Ribosomal
Angiostrongylus cantonensis; Genetic diversity; Mitochondrial genome; Phylogenetics; Rat lungworm; Transfer RNA