Ultrastructural Analysis of Drosophila Ovaries by Electron Microscopy.
The Drosophila melanogaster ovary is a powerful, genetically tractable system through which one can elucidate the principles underlying cellular function and organogenesis in vivo. In order to understand the intricate process of oogenesis at the subcellular level, microscopic analysis with the highest possible resolution is required. In this chapter, we describe the preparation of ovaries for ultrastructural analysis using transmission electron microscopy and focused ion beam scanning electron microscopy. We discuss and provide protocols for chemical fixation of Drosophila ovaries that facilitate optimal imaging with particular attention paid to preserving and resolving mitochondrial membrane morphology and structure.
Howard Hughes Medical Institute (HHMI), New York University School of Medicine, New York, NY, 10016, USA.
Thomas R
TR
Hurd
Carlos G
CG
Sanchez
Felipe K
FK
Teixeira
Chris
C
Petzold
Kristen
K
Dancel-Manning
Ju-Yu S
JY
Wang
Ruth
R
Lehmann
Feng-Xia A
FX
Liang
D000818:Animals; D004331:Drosophila melanogaster; D005260:Female; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D009866:Oogenesis; D010053:Ovary / Q000648:ultrastructure*