Interrogation of Cellular Innate Immunity by Diamond-Nanoneedle-Assisted Intracellular Molecular Fishing.
Understanding intracellular signaling cascades and network is one of the core topics in modern biology. Novel tools based on nanotechnologies have enabled probing and analyzing intracellular signaling with unprecedented sensitivity and specificity. In this study, we developed a minimally invasive method for in situ probing specific signaling components of cellular innate immunity in living cells. The technique was based on diamond-nanoneedle arrays functionalized with aptamer-based molecular sensors, which were inserted into cytoplasmic domain using a centrifugation controlled process to capture molecular targets. Simultaneously, these diamond-nanoneedles also facilitated the delivery of double-strand DNAs (dsDNA90) into cells to activate the pathway involving the stimulator of interferon genes (STING). We showed that the nanoneedle-based biosensors can be successfully utilized to isolate transcriptional factor, NF-κB, from intracellular regions without damaging the cells, upon STING activation. By using a reversible protocol and repeated probing in living cells, we were able to examine the singling dynamics of NF-κB, which was quickly translocated from cytoplasm to nucleus region within ∼40 min of intracellular introduction of dsDNA90 for both A549 and neuron cells. These results demonstrated a novel and versatile tool for targeted in situ dissection of intracellular signaling, providing the potential to resolve new sights into various cellular processes.
Zixun
Z
Wang
Functional Thin Films Research Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences , Shenzhen 518055, China.
Yang
Y
Yang
Zhen
Z
Xu
Ying
Y
Wang
Wenjun
W
Zhang
Shenzhen Research Institute, City University of Hong Kong , Shenzhen 518057, China.
Peng
P
Shi
D000818:Animals; D045744:Cell Line, Tumor; D018130:Diamond; D006801:Humans; D007113:Immunity, Innate; D008855:Microscopy, Electron, Scanning; D009339:Needles
D018130:Diamond
Diamond nanoneedles; STING activation; in situ detection; innate immunity; intracellular sensing