A highly efficient urea detection using flower-like zinc oxide nanostructures.
A novel matrix based on flower-like zinc oxide nanostructures (ZnONF) has been fabricated using hydrothermal method and exploited successfully for the development of urea biosensor. Urease (Urs) is physically immobilized onto the ZnO nanostructure matrix synthesized over platinized silicon substrate. The surface morphology and crystallographic structure of the as-grown ZnONF have been characterized using a scanning electron microscope (SEM) and X-ray diffraction (XRD) techniques. The fabricated amperometric biosensor (Urs/ZnONF/Pt/Ti/Si) exhibits a linear sensing response towards urea over the concentration range 1.65 mM to 16.50mM with an enhanced sensitivity (~132 μA/mM/cm(2)) and a fast response time of 4s. The relatively low value of Michaelis-Menten constant (Km) of 0.19 mM confirms the high affinity of the immobilized urease on the nanostructured ZnONF surface towards its analyte (urea). The obtained results demonstrate that flower-like ZnO nanostructures serve as a promising matrix for the realization of efficient amperometric urea biosensor with enhanced response characteristics.
Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India.
Manvi
M
Tak
Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India.
Vinay
V
Gupta
Department of Physics, Miranda House, University of Delhi, Delhi 110007, India. Electronic address: monikatomar@gmail.com.
Monika
M
Tomar
D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D014508:Urea / Q000032:analysis*; D014510:Urease / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry*
D004800:Enzymes, Immobilized; D014508:Urea; D014510:Urease; D015034:Zinc Oxide
Biosensor; Flower-like nanostructures; Hydrothermal method; Urea; Zinc oxide