Rapid and accurate detection of Escherichia coli growth by fluorescent pH-sensitive organic nanoparticles for high-throughput screening applications.
Rapid detection of bacterial growth is an important issue in the food industry and for medical research. Here we present a novel kind of pH-sensitive fluorescent nanoparticles (FANPs) that can be used for the rapid and accurate real-time detection of Escherichia coli growth. These organic particles are designed to be non-toxic and highly water-soluble. Here we show that the coupling of pH sensitive fluoresceinamine to the nanoparticles results in an increased sensitivity to changes in pH within a physiologically relevant range that can be used to monitor the presence of live bacteria. In addition, these FANPs do not influence bacterial growth and are stable over several hours in a complex medium and in the presence of bacteria. The use of these FANPs allows for continuous monitoring of bacterial growth via real-time detection over long time scales in small volumes and can thus be used for the screening of a large number of samples for high-throughput applications such as screening for the presence of antibiotic resistant strains.
PPSM, CNRS UMR 8531, ENS-Cachan, 61 av President Wilson, 94230 Cachan, France; LBPA, CNRS UMR 8113, ENS-Cachan, 61 av President Wilson, 94230 Cachan, France.
Yang
Y
Si
PPSM, CNRS UMR 8531, ENS-Cachan, 61 av President Wilson, 94230 Cachan, France.
Chloé
C
Grazon
PPSM, CNRS UMR 8531, ENS-Cachan, 61 av President Wilson, 94230 Cachan, France.
Gilles
G
Clavier
Sorbonne Universités, UPMC Univ Paris 06, CNRS, Institut Parisien de Chimie Moléculaire, Equipe Chimie des Polymères, 4 Place Jussieu, F-75005 Paris, France.
Jutta
J
Rieger
PPSM, CNRS UMR 8531, ENS-Cachan, 61 av President Wilson, 94230 Cachan, France.
Jean-Frédéric
JF
Audibert
LBPA, CNRS UMR 8113, ENS-Cachan, 61 av President Wilson, 94230 Cachan, France. Electronic address: sclavi@lbpa.ens-cachan.fr.
Bianca
B
Sclavi
PPSM, CNRS UMR 8531, ENS-Cachan, 61 av President Wilson, 94230 Cachan, France. Electronic address: rachel.meallet-renault@u-psud.fr.
Rachel
R
Méallet-Renault
D015374:Biosensing Techniques / Q000379:methods*; D004926:Escherichia coli / Q000254:growth & development / Q000302:isolation & purification*; D005453:Fluorescence; D057166:High-Throughput Screening Assays / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry
Bacteria; Fluoresceinamine; High-throughput screening (HTS); Organic nanoparticles; Rapid detection; pH detection