Controlled antibiotic-releasing Antheraea assama silk fibroin suture for infection prevention and fast wound healing.
BACKGROUND
The quest for developing silk fibroin as a biomaterial for drug release systems continues to draw research interest owing to its impressive mechanical properties as well as biocompatibility and biodegradability. The aim of this study is to develop low-temperature O2 plasma-treated muga (Antheraea assama) silk fibroin (AASF) yarn impregnated with amoxicillin trihydrate as controlled antibiotic-releasing suture (AASF/O2/AMOX) for preventing postoperative site bacterial infection and fast wound healing.


METHODS
In this experimental study, AASF and AASF/O2/AMOX sutures are used to close the surgical wounds of adult male Wistar rats of 4 months old and weighing 200-230 g.


RESULTS
Surface hydrophilicity induced by O2 plasma results in an increase in drug-impregnation efficiency of AASF/O2 yarn by 16.7%. In vitro drug release profiles show continuous and prolonged release of AMOX from AASF/O2/AMOX yarn up to 336 hours. In vitro hemolysis assay reveals that O2 plasma treatment and subsequent impregnation of AMOX do not affect the heertetmocompatibility of AASF yarn. The AASF/O2/AMOX yarn proves to be effective for in vitro growth inhibition of Staphylococcus aureus and Escherichia coli, whereas AASF offers no antibacterial activity against both types of bacteria. In vivo histopathology studies and colony-forming unit count data revealed accelerated wound healing activity of AASF/O2/AMOX over AASF yarn through rapid synthesis and proliferation of collagen, hair follicle, and connective tissues.


CONCLUSIONS
Outcomes of this work clearly demonstrate the potential use of AASF/O2/AMOX yarn as a controlled antibiotic-releasing suture biomaterial for superficial surgical applications.
Department of Physics, Tezpur University, Assam, India. Electronic address: arupjchoudhury@gmail.com.
Arup Jyoti
AJ
Choudhury
Physical Sciences Division, Institute of Advanced Study in Science and Technology, Assam, India.
Dolly
D
Gogoi
Physical Sciences Division, Institute of Advanced Study in Science and Technology, Assam, India.
Joyanti
J
Chutia
Drug Discovery Laboratory, Institute of Advanced Study in Science and Technology, Assam, India.
Raghuram
R
Kandimalla
Drug Discovery Laboratory, Institute of Advanced Study in Science and Technology, Assam, India.
Sanjeeb
S
Kalita
Drug Discovery Laboratory, Institute of Advanced Study in Science and Technology, Assam, India.
Jibon
J
Kotoky
Molecular Biology and Microbial Biotechnology Laboratory, Institute of Advanced Study in Science and Technology, Assam, India.
Yogesh B
YB
Chaudhari
Molecular Biology and Microbial Biotechnology Laboratory, Institute of Advanced Study in Science and Technology, Assam, India.
Mojibur R
MR
Khan
Department of Pathology, Hayat Hospital, Assam, India.
Kasturi
K
Kalita
D000658:Amoxicillin / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D008297:Male; D009036:Moths; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D047011:Silk; D013530:Surgical Wound Infection / Q000517:prevention & control*; D013536:Suture Techniques; D013537:Sutures; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D047011:Silk; D000658:Amoxicillin