The complete mitochondrial genome of Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae).
We determined the complete mitochondrial genome (mitogenome) sequence of Cryptolestes ferrugineus (GenBank accession number KT182067) by the long PCR and primer walking method. The mitochondrial genome is a typical circular DNA molecule of 15 511 bp in length, and contains 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a A + T-rich region (D-loop). The order of 37 genes was typical of insect mitochondrial DNA sequences described to date. The base composition of the genome is A (39.17%), T (37.24%), C (14.22%), and G (9.37%) with an A + T-rich hallmark as that of other invertebrate mitochondrial genomes. All protein-coding genes start with ATN codon and terminate with the stop codon T (AA) or TAG. The A + T-rich region is located between 12S rRNA and tRNA(Ile). In this study, the phylogenetic relationships of Coleoptera species were constructed based on the nucleotides sequences of 13 PCGs of mitogenomes. The molecular-based phylogeny supported the traditional morphological classification on relationships within Coleoptera species.
a College of Life Science, Nanchang University , Nanchang , China.
Tian Yi
TY
Sun
a College of Life Science, Nanchang University , Nanchang , China.
Lei
L
Li
a College of Life Science, Nanchang University , Nanchang , China.
Tianrong
T
Xin
a College of Life Science, Nanchang University , Nanchang , China.
Yayu
Y
Wang
a College of Life Science, Nanchang University , Nanchang , China.
Bin
B
Xia
D000818:Animals; D001482:Base Composition; D018387:Codon, Initiator; D018388:Codon, Terminator; D001517:Coleoptera / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D059646:Genome Size; D054629:Genome, Mitochondrial; D019476:Insect Proteins / Q000235:genetics; D024101:Mitochondrial Proteins / Q000235:genetics; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D000073336:Whole Genome Sequencing
D018387:Codon, Initiator; D018388:Codon, Terminator; D004272:DNA, Mitochondrial; D019476:Insect Proteins; D024101:Mitochondrial Proteins; D012335:RNA, Ribosomal; D012343:RNA, Transfer
Cryptolestes ferrugineus; mitochondrial genome; phylogenetic relationships