[Functionalization of screen printed electrodes with organic-inorganic hybrid nano-composites for bio-sensing applications].
New types of organic-inorganic hybrid nanocomposites based on nanosized Titanium (IV) oxide TiO2 (<100 nm particle size) and carbon nanotubes (CNT, outer diameter 10-15 nm, inner diamentre 2-6 nm, length 0.1-10 µm) and phosphatidilcholine were elaborated for improvement of analytical characteristics of screen printed electrodes. These nanomaterials were employed as an interface for the immobilization of skeletal myoglobin. Electrochemical behavior of myoglobin on such interfaces was characterized with cyclic voltammetry (CV) and square wave voltammetry (SWV). Direct unmediated electron transfer between myoglobin and electrodes modified with organic-inorganic hybrid nanocomposites was registered. TiO2 film and CNT film are biocompartible nanomaterials for myoglobin as was demonstrated with UV-Vis spectra. The midpoint potential of Fe3+/Fe2+ pair of myoglobin corresponded to Е1/2=-0,263 V for CNT film, and Е1/2=-0,468 V for TiO2 nanocomposite (vs. Ag/AgCl reference electrode).
Institute of Biomedical Chemistry, Moscow, Russia.
V V
VV
Shumyantseva
Institute of Biomedical Chemistry, Moscow, Russia.
T V
TV
Bulko
Institute of Biomedical Chemistry, Moscow, Russia.
A V
AV
Kuzikov
Analytical Chemistry Division, CSIR-North East Institute of Science & Technology, Jorhat, Assam, India.
R
R
Khan
Institute of Biomedical Chemistry, Moscow, Russia.
A I
AI
Archakov
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques; D004566:Electrodes; D004583:Electrons; D006736:Horses; D055767:Immobilized Proteins / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D011327:Printing; D014025:Titanium / Q000737:chemistry*
D055767:Immobilized Proteins; D009211:Myoglobin; D037742:Nanotubes, Carbon; D010713:Phosphatidylcholines; C009495:titanium dioxide; D014025:Titanium
Titanium oxyde; carbon nanotubes; electron transfer; enzyme electrodes; myoglobin; nanomaterials