Diagnostic performance of a new vimentin-derived ACPA (CCP high sensitive) in patients with rheumatoid arthritis.
Autoantibodies are a common feature of rheumatoid arthritis (RA), and their detection is used as a diagnostic tool in medical practice. Rheumatoid factor (RF) and/or anti-citrullinated protein antibodies (ACPA) detection in patients' sera are now included in 2010 ACR/EULAR criteria for RA diagnosis. In this study, we evaluated a new vimentin-derived ACPA ELISA, the anti-cyclic citrullinated peptide high sensitive (CCP hs) test, and we compared its performance with the RF IgM and anti-CCP3 tests on a French multicenter cohort of 84 RA patients, 107 non-RA patients and 100 healthy controls. Sensitivities for RA diagnosis were 71.4, 84.5 and 64.3 % and specificities were 88.4, 86.9 and 87.3 % for CCP hs, CCP3 and RF IgM, respectively. There was a moderate correlation between CCP hs and CCP3 titers (Pearson's r = 0.43; p < 0.0001). These results support the contention that anti-CCP hs antibodies are new reliable ACPA with high specificity for RA.
Service d'Immunologie, Pôle de Biologie, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille, 147, Bd Baille, 13005, Marseille, France. daniel.bertin@ap-hm.fr.|NICN UMR 7259, Aix-Marseille Université, CNRS, 13916, Marseille, France. daniel.bertin@ap-hm.fr.
Daniel
D
Bertin
Institut d'Immunologie - Centre de Biologie Pathologie et Génétique, CHRU Lille, 59037, Lille, France.|LIRIC, UMR 995, INSERM, 59000, Lille, France.|EA 2686, Université Lille Nord de France, 59000, Lille, France.
Sylvain
S
Dubucquoi
Laboratoire d'Immunologie, Plateau Technique de Biologie, CHU Dijon, 21000, Dijon, France.
Daniela
D
Lakomy
Institut d'Immunologie - Centre de Biologie Pathologie et Génétique, CHRU Lille, 59037, Lille, France.
Anne-Sophie
AS
Deleplancque
Service d'Immunologie, Pôle de Biologie, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille, 147, Bd Baille, 13005, Marseille, France.|NICN UMR 7259, Aix-Marseille Université, CNRS, 13916, Marseille, France.
Sophie
S
Desplat-Jégo
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000175:diagnosis* / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010456:Peptides, Cyclic / Q000276:immunology*; D012372:ROC Curve; D012680:Sensitivity and Specificity
D001323:Autoantibodies; D010456:Peptides, Cyclic; C487763:cyclic citrullinated peptide
ACPA; Anti-CCP antibodies; Anti-citrullinated protein antibodies; CCP hs (high sensitive); Rheumatoid arthritis