β1-and β2-adrenoceptors in hippocampal CA3 region are required for long-term memory consolidation in rats.
The existence of β-adrenoceptors (ARs) in the hippocampus and the importance of β-ARs in regulating synaptic plasticity and learning/memory function are well documented. As known, β-ARs in area cornu ammonis 1 (CA1) are involved in regulating memory consolidation. However, little is known about the functional roles of the β-ARs subtypes, β1- and β2-ARs, in the hippocampal cornu ammonis 3 (CA3) region. To address this question, we firstly locally infused the β1- or β2-ARs antagonist into the CA3 region and observed that blockage of either β1-AR or β2-AR impaired long-term contextual fear memory and water-maze spatial memory. We also found that, following the contextual fear conditioning, the expression of β1-AR in the CA3 region significantly increased, whereas β2-AR was unchanged. Then intra-CA3 infusion of recombinant lentiviral RNAi vectors for β1 or β2-ARs also produced deficit in contextual memory consolidation. Taken together, the results suggested that the β1- and β2-ARs in the CA3 region were involved in hippocampus dependent memory consolidation.
Center for Neuropsychiatric Diseases, Institute of Life Science, Nanchang University, Nanchang 330031, China.
Jian
J
Zheng
Center for Neuropsychiatric Diseases, Institute of Life Science, Nanchang University, Nanchang 330031, China. Electronic address: luofei@ncu.edu.cn.
Fei
F
Luo
Institute of Neurobiology & State Key Laboratory of Medical Neurobiology, Institutes of Brain Science, Fudan University, Shanghai 200032, China.
Nan-nan
NN
Guo
Center for Neuropsychiatric Diseases, Institute of Life Science, Nanchang University, Nanchang 330031, China.
Zong-yue
ZY
Cheng
Center for Neuropsychiatric Diseases, Institute of Life Science, Nanchang University, Nanchang 330031, China; Center for Neuropsychiatric Diseases, Institute of Life Science, Nanchang University, Nanchang 330031, China.
Bao-ming
BM
Li
D058671:Adrenergic beta-1 Receptor Antagonists / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D056654:CA3 Region, Hippocampal / Q000187:drug effects / Q000378:metabolism*; D003213:Conditioning, Psychological / Q000502:physiology; D005239:Fear; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D000069077:Memory Consolidation / Q000187:drug effects / Q000502:physiology*; D057567:Memory, Long-Term / Q000187:drug effects / Q000502:physiology*; D008790:Metoprolol / Q000494:pharmacology; D011412:Propanolamines / Q000494:pharmacology; D034622:RNA Interference / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018342:Receptors, Adrenergic, beta-1 / Q000235:genetics / Q000378:metabolism*; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics / Q000378:metabolism*; D014161:Transduction, Genetic
D058671:Adrenergic beta-1 Receptor Antagonists; D011412:Propanolamines; D012333:RNA, Messenger; D018342:Receptors, Adrenergic, beta-1; D018343:Receptors, Adrenergic, beta-2; C026777:ICI 118551; D008790:Metoprolol
CA3; Contextual fear memory; Rat; Spatial memory; β1-AR; β2-AR