miR-372 suppresses tumour proliferation and invasion by targeting IGF2BP1 in renal cell carcinoma.
OBJECTIVE
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that regulate proteins and mRNAs for degradation or translational suppression. Up to now, the role of miR-372 in renal cell carcinoma has remained unknown; in this study, we have aimed to reveal its functional importance in this tumour.


METHODS
qRT-PCR was performed to measure expression levels of miR-372 in renal cell carcinoma cell lines and tissues. CCK-8 and an invasion assay were performed to measure its functional role. Luciferase assays, qRT-PCR and western blotting were performed to discover miR-372's target gene.


RESULTS
We demonstrated that miRNA-372 was down-regulated in renal cell carcinoma cell lines and tissue specimens; its over-expression inhibited cell proliferation and invasion. Moreover, we showed that miRNA-372 repressed insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) expression by directly interacting with its putative binding site at the 3'-UTR. Furthermore, ectopic expression of IGF2BP1 significantly reversed suppression of cell proliferation and invasion caused by miR-372 over-expression.


CONCLUSIONS
Our data indicated that miR-372 seemed to function as a tumour suppressor in renal cell carcinoma progression by inhibiting the IGF2BP1 expression.
The Institute of Translational Medicine, Nanchang University, Jiangxi, 330031, China.
Xuan
X
Huang
College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, 330045, China.
Mingjie
M
Huang
College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, 330045, China.
Lingbao
L
Kong
The Institute of Translational Medicine, Nanchang University, Jiangxi, 330031, China.
Yong
Y
Li
D020413:3' Untranslated Regions; D001483:Base Sequence; D001665:Binding Sites; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
D020413:3' Untranslated Regions; C406144:IGF2BP1 protein, human; C561021:MIRN372 microRNA, human; D035683:MicroRNAs; D016601:RNA-Binding Proteins