Detection of thrombin based on aptamer isothermal exponential signal amplification technique.
In this report, a strategy based on an aptameric molecule switch to realize homogeneous and isothermal signal exponential amplification and protein detection has been described. This method was simple and sensitive, allowing detection of as low as 2.6 × 10(-12) M thrombin.
Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China. chemsxt1990@163.com.
Xiaotong
X
Shen
Menghua
M
Zhang
Shuyan
S
Niu
Chao
C
Shi
D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D006801:Humans; D055029:Inverted Repeat Sequences; D057230:Limit of Detection; D013696:Temperature; D013917:Thrombin / Q000032:analysis* / Q000378:metabolism
D052157:Aptamers, Nucleotide; D013917:Thrombin