Biogeographical distribution of Rimicaris exoculata resident gut epibiont communities along the Mid-Atlantic Ridge hydrothermal vent sites.
Rimicaris exoculata is a deep-sea hydrothermal vent shrimp whose enlarged gill chamber houses a complex trophic epibiotic community. Its gut harbours an autochthonous and distinct microbial community. This species dominates hydrothermal ecosystem megafauna along the Mid-Atlantic Ridge, regardless of contrasting geochemical conditions prevailing in them. Here, the resident gut epibiont community at four contrasted hydrothermal vent sites (Rainbow, TAG, Logatchev and Ashadze) was analysed and compiled with previous data to evaluate the possible influence of site location, using 16S rRNA surveys and microscopic observations (transmission electron microscopy, scanning electron microscopy and fluorescence in situ hybridization analyses). Filamentous epibionts inserted between the epithelial cell microvilli were observed on all examined samples. Results confirmed resident gut community affiliation to Deferribacteres, Mollicutes, Epsilonproteobacteria and to a lesser extent Gammaproteobacteria lineages. Still a single Deferribacteres phylotype was retrieved at all sites. Four Mollicutes-related operational taxonomic units were distinguished, one being only identified on Rainbow specimens. The topology of ribotype median-joining networks illustrated a community diversification possibly following demographic expansions, suggesting a more ancient evolutionary history and/or a larger effective population size at Rainbow. Finally, the gill chamber community distribution was also analysed through ribotype networks based on sequences from R. exoculata collected at the Rainbow, Snake Pit, TAG, Logatchev and Ashadze sites. Results allow the refining of hypotheses on the epibiont role and transmission pathways.
Ifremer, Centre de Brest, Laboratoire de Microbiologie des Environnements Extrêmes, REM/EEP/LM2E, UMR 6197 Ifremer-CNRS-UBO, BP 70, 29280 Plouzané, France.
Lucile
L
Durand
Ifremer, Centre de Brest, Laboratoire de Microbiologie des Environnements Extrêmes, REM/EEP/LM2E, UMR 6197 Ifremer-CNRS-UBO, BP 70, 29280 Plouzané, France.
Marie
M
Roumagnac
Ifremer, Centre de Brest, Laboratoire de Microbiologie des Environnements Extrêmes, REM/EEP/LM2E, UMR 6197 Ifremer-CNRS-UBO, BP 70, 29280 Plouzané, France.
Valérie
V
Cueff-Gauchard
Université de Brest, Laboratoire de Microbiologie des Environnements Extrêmes, UMR 6197 Ifremer-CNRS-UBO, Technopôle Iroise, 4 place Nicolas Copernic, 29280 Plouzané, France.
Cyrielle
C
Jan
CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, UMR 6197 Ifremer-CNRS-UBO, Technopôle Iroise, 4 place Nicolas Copernic, 29280 Plouzané, France.
Mathieu
M
Guri
Ifremer, Centre de Brest, Laboratoire de Microbiologie des Environnements Extrêmes, REM/EEP/LM2E, UMR 6197 Ifremer-CNRS-UBO, BP 70, 29280 Plouzané, France.
Claire
C
Tessier
Ifremer, Centre de Brest, Laboratoire de Microbiologie des Environnements Extrêmes, REM/EEP/LM2E, UMR 6197 Ifremer-CNRS-UBO, BP 70, 29280 Plouzané, France.
Marine
M
Haond
Ifremer, Centre de Brest, Laboratoire Environnements Profonds, REM/EEP/LEP, 29280 Plouzané, France.
Philippe
P
Crassous
UMR CNRS 7208 BOREA, Equipe aux Milieux Extrêmes, Université Pierre et Marie Curie Paris VI, 7 Quai Saint Bernard, 75252 Paris cedex 05, France.
Magali
M
Zbinden
Ifremer, Centre de Brest, Laboratoire Environnements Profonds, REM/EEP/LEP, 29280 Plouzané, France.
Sophie
S
Arnaud-Haond
Ifremer, Centre de Brest, Laboratoire de Microbiologie des Environnements Extrêmes, REM/EEP/LM2E, UMR 6197 Ifremer-CNRS-UBO, BP 70, 29280 Plouzané, France marie.anne.cambon@ifremer.fr.
Marie-Anne
MA
Cambon-Bonavita
D000818:Animals; D001267:Atlantic Ocean; D044822:Biodiversity; D033364:Decapoda / Q000382:microbiology*; D017753:Ecosystem; D020565:Epsilonproteobacteria / Q000235:genetics; D020563:Gammaproteobacteria / Q000235:genetics; D000069196:Gastrointestinal Microbiome / Q000235:genetics*; D005880:Gills / Q000382:microbiology*; D060086:Hydrothermal Vents / Q000382:microbiology*; D017404:In Situ Hybridization, Fluorescence; D009174:Mycoplasma / Q000235:genetics; D058974:Phylogeography; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013559:Symbiosis
D012336:RNA, Ribosomal, 16S
epibiont; extreme environments; network analyses; phylogeography