Micrometam C Protects against Oxidative Stress in Inflammation Models in Zebrafish and RAW264.7 Macrophages.
Micrometam C is a core of novel marine compound isolated from the mangrove associates Micromelum falcatum. In this study, we investigated the protective effects of micrometam C in inflammation models in the transgenic zebrafish line Tg (corola: eGFP) and RAW264.7 macrophages. We found that micrometam C significantly suppressed the migration of immune cells in tail-cutting-induced inflammation in transgenic zebrafish and reduced lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) in both zebrafish and macrophages. In addition, micrometam C also restored LPS-induced reduction of endogenous antioxidants, such as catalase (CAT), glutathione (GSH) and superoxide dismutase (SOD). The protective effects of micrometam C were in parallel to its inhibition of NADPH oxidase and nuclear factor-kappa-binding (NF-κB) activity. Thus, the present results demonstrate that micrometam C protects against LPS-induced inflammation possibly through its antioxidant property.
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. kyrintang@163.com.
Hao
H
Tang
Department of Health Care Clinic, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. gehui2009gh@126.com.
Hui
H
Ge
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. psyczb@aliyun.com.
Zhi-Bin
ZB
Chen
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. luoxm163@sina.com.
Xiong-Ming
XM
Luo
Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. jisufengjuan@163.com.
Feng-Juan
FJ
Su
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. xuyw8591@126.com.
Yan-Bing
YB
Liang
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. leezhenyu99@163.com.
Zhen-Yu
ZY
Li
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. wjg114500@163.com.
Jing-Guo
JG
Wu
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. yangqin804@163.com.
Qing
Q
Yang
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. 164555182@qq.com.
Li-Jin
LJ
Zeng
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. mazhongfu05@sohu.com.
Zhong-Fu
ZF
Ma
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology*; D000975:Antioxidants; D002460:Cell Line; D003373:Coumaric Acids / Q000737:chemistry* / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy*; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages; D051379:Mice; D015394:Molecular Structure; D019255:NADPH Oxidases / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D016897:Respiratory Burst; D013623:Tail; D015027:Zebrafish
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D003373:Coumaric Acids; D008070:Lipopolysaccharides; D016328:NF-kappa B; C000608073:micrometam C; D019255:NADPH Oxidases
NADPH oxidase; NF-κB; inflammatory; micrometam C; zebrafish