Metabolic characterization of asthenozoospermia using nontargeted seminal plasma metabolomics.
BACKGROUND
Asthenozoospermia (AS) is a common cause of male infertility. Due to the limitation of routine semen analysis, metabolic alterations associated with the disease are unclear. We applied a metabolic profiling strategy as a surrogate method to accurately assess and provide new insights into the etiologies of asthenozoospermia.


METHODS
Seminal plasma samples from patients diagnosis with asthenozoospermia (n=33) and healthy subjects (n=30) were investigated using a nontargeted metabolomics approach based on proton nuclear magnetic resonance ((1)H NMR) spectroscopy. The spectral data were then subjected to multivariate and univariate analyses to identify metabolites that were correlated with asthenozoospermia. The disturbed metabolic pathways which the biomarkers were involved in were analyzed.


RESULTS
Nineteen metabolites including up-regulation or down-regulation of several amino acids, changes in lipids metabolism, phospholipids (choline) metabolism, cholesterol metabolism, nucleoside metabolism, the Krebs cycle and energy metabolism were identified and associated with asthenozoospermia. In particular, the elevation of oxysterols such as 5α-cholesterol and 7-ketocholesterol in seminal plasma of patients with asthenozoospermia was an important finding, indicating the important role of oxidative stress in the mechanism of asthenozoospermia.


CONCLUSIONS
The excellent performance of this metabolomics approach offer a highly novel means of etiological diagnosis of asthenozoospermia.
Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, China.
Xiaoli
X
Zhang
Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, China.
Ruiying
R
Diao
State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
Xinyue
X
Zhu
Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, China.
Zesong
Z
Li
Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, China. Electronic address: zmcai13602530322@163.com.
Zhiming
Z
Cai
D000328:Adult; D053627:Asthenozoospermia / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D006801:Humans; D008297:Male; D055432:Metabolomics; D012661:Semen / Q000378:metabolism*; D055815:Young Adult
D015415:Biomarkers
Asthenozoospermia; Metabolomics; Proton nuclear magnetic resonance ((1)H NMR) spectroscopy; Seminal plasma