Effect of a Novel Stent on Re-Endothelialization, Platelet Adhesion, and Neointimal Formation.
OBJECTIVE
Vascular endothelial-cadherin (VE-cadherin) is specifically expressed by outgrowth endothelial cells (OECs). Zwitterionic stent showed high antifouling and excellent blood compatibility. Therefore, we hypothesized that anti-VE-cadherin antibody-coated zwitterionic stents (VE-cad-Z stents) would promote re-endothelialization, reduce neointimal formation, and resist thrombus.


METHODS
VE-cad-Z stents were examined using platelet adhesion test, platelet activation, and OEC capture ability in vitro. In vivo effect of VE-cad-Z stents on re-endothelialization, thrombus-resistance, and neointima hyperplasia was investigated in left common carotid arteries of rabbits (n=15).


RESULTS
In vitro, VE-cad-Z stents showed better platelet-resistance and OEC-capture ability (DNA concentration: 297.23±22.71 versus 67.49±15.26 ng/µL, P＜0.01). In vivo, VE-cad-Z stents exhibited better patency rate than bare metal stents (BMS) (15/15 versus 12/15), and it significantly reduced platelet adhesion and neointima formation (neointima area: 1.13±0.05 versus 1.00±0.05mm(2), P＜0.01 and 3.04±0.11versus 1.05±0.06mm(2), P＜0.01, at 3 and 30 days, respectively; % stenosis: 20.99±0.98 versus 18.72±0.97, P＜0.01 and 56.46±2.20 versus 19.45±1.24, P＜0.01, at 3 and 30 days, respectively).


CONCLUSIONS
These data suggested that VE-cad-Z stents could specifically capture OECs, consequently promote endothelial healing, and also reduce platelet adhesion and neointima formation.
Department of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School.
Hanfei
H
Tang
Qi
Q
Wang
Xiaobo
X
Wang
Jianping
J
Zhou
Mengjiao
M
Zhu
Tong
T
Qiao
Changjian
C
Liu
Chun
C
Mao
Min
M
Zhou
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D001792:Blood Platelets / Q000166:cytology*; D015820:Cadherins / Q000378:metabolism*; D017536:Carotid Artery, Common / Q000473:pathology; D002448:Cell Adhesion; D049109:Cell Proliferation; D054855:Drug-Eluting Stents; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000473:pathology*; D006801:Humans; D006965:Hyperplasia / Q000473:pathology; D007249:Inflammation; D008297:Male; D008856:Microscopy, Fluorescence; D058426:Neointima / Q000473:pathology*; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D011817:Rabbits; D013927:Thrombosis / Q000473:pathology; D000099152:Cadherin 5
D015703:Antigens, CD; D015820:Cadherins; D000099152:Cadherin 5