Molecular characterization of carbapenem-resistant Pseudomonas aeruginosa strains isolated from patients with urinary tract infections in Southern Poland.
Due to the clinical threat posed by multidrug-resistant (MDR) Pseudomonas aeruginosa and importance of virulence factors produced in infection, 21 carbapenem-resistant P. aeruginosa were analyzed. 42.8% metallo-beta-lactamases-positive strains were identified. 85.7% of strains were meropenem resistant. 14.2% of strains were MDR; 38%, extensively drug-resistant (XDR). ExoY was present in all strains; exoT, in 95.2%; exoS, in 90.5%; exoU, in 47.6%. Eight XDR strains were typed using multilocus sequence typing: 4 as ST235, 2 as ST260, 2 as ST654 and ST234. MDR P. aeruginosa were isolated from hospitalized patients and among those from the community. Our study demonstrates the serious clinical issues posed by MDR P. aeruginosa and underscores the need for new treatment.
Department of Microbiology, Jagiellonian University Medical College, Krakow, Poland. Electronic address: monika.pobiega@gmail.com.
Monika
M
Pobiega
Institute of Dentistry, Department of Dental Prophylaxis and Experimental Dentistry, Jagiellonian University Medical College, Krakow, Poland.
Joanna
J
Maciąg
Department of Microbiology, Jagiellonian University Medical College, Krakow, Poland.
Agnieszka
A
Chmielarczyk
Department of Microbiology, Jagiellonian University Medical College, Krakow, Poland.
Dorota
D
Romaniszyn
Department of Microbiology, Analytical and Microbiological Laboratory of Ruda Slaska, KORLAB NZOZ, Ruda Slaska, Poland.
Monika
M
Pomorska-Wesolowska
Higher School of Medicine in Sosnowiec, Poland.
Grzegorz
G
Ziolkowski
Department of Microbiology, Jagiellonian University Medical College, Krakow, Poland.
Piotr B
PB
Heczko
Department of Microbiology, Jagiellonian University Medical College, Krakow, Poland.
Malgorzata
M
Bulanda
Department of Microbiology, Jagiellonian University Medical College, Krakow, Poland.
Jadwiga
J
Wojkowska-Mach
D000293:Adolescent; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism; D015780:Carbapenems / Q000494:pharmacology*; D016000:Cluster Analysis; D005260:Female; D005798:Genes, Bacterial; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D011044:Poland / Q000453:epidemiology; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D014552:Urinary Tract Infections / Q000453:epidemiology / Q000382:microbiology*; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C063912:carbapenemase
Carbapenem-resistance; MLST; Pseudomonas aeruginosa; exo