Global analysis of asymmetric RNA enrichment in oocytes reveals low conservation between closely related Xenopus species.
RNAs that localize to the vegetal cortex during Xenopus laevis oogenesis have been reported to function in germ layer patterning, axis determination, and development of the primordial germ cells. Here we report on the genome-wide, comparative analysis of differentially localizing RNAs in Xenopus laevis and Xenopus tropicalis oocytes, revealing a surprisingly weak degree of conservation in respect to the identity of animally as well as vegetally enriched transcripts in these closely related species. Heterologous RNA injections and protein binding studies indicate that the different RNA localization patterns in these two species are due to gain/loss of cis-acting localization signals rather than to differences in the RNA-localizing machinery.
Institute of Developmental Biochemistry, University Medical Center Göttingen, 37077 Göttingen, Germany mclauss1@gwdg.de tpieler@gwdg.de.
Maike
M
Claußen
Microarray and Deep-Sequencing Core Facility, University Medical Center Göttingen, 37077 Göttingen, Germany.
Thomas
T
Lingner
Microarray and Deep-Sequencing Core Facility, University Medical Center Göttingen, 37077 Göttingen, Germany.
Claudia
C
Pommerenke
Microarray and Deep-Sequencing Core Facility, University Medical Center Göttingen, 37077 Göttingen, Germany.
Lennart
L
Opitz
Microarray and Deep-Sequencing Core Facility, University Medical Center Göttingen, 37077 Göttingen, Germany.
Gabriela
G
Salinas
Institute of Developmental Biochemistry, University Medical Center Göttingen, 37077 Göttingen, Germany mclauss1@gwdg.de tpieler@gwdg.de.
Tomas
T
Pieler
D000818:Animals; D005260:Female; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D009866:Oogenesis / Q000235:genetics; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017423:Sequence Analysis, RNA; D013045:Species Specificity; D059467:Transcriptome; D014981:Xenopus / Q000235:genetics* / Q000378:metabolism; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D029867:Xenopus Proteins; D012313:RNA