Antitumour Activity of the Microencapsulation of Annona vepretorum Essential Oil.
Annona vepretorum Mart. (Annonaceae), popularly known as 'bruteira', has nutritional and medicinal uses. This study investigated the chemical composition and antitumour potential of the essential oil of A. vepretorum leaf alone and complexed with β-cyclodextrin in a microencapsulation. The essential oil was obtained by hydrodistillation using a Clevenger-type apparatus and analysed using GC-MS and GC-FID. In vitro cytotoxicity of the essential oil and some of its major constituents in tumour cell lines from different histotypes was evaluated using the alamar blue assay. Furthermore, the in vivo efficacy of essential oil was demonstrated in mice inoculated with B16-F10 mouse melanoma. The essential oil included bicyclogermacrene (35.71%), spathulenol (18.89%), (E)-β-ocimene (12.46%), α-phellandrene (8.08%), o-cymene (6.24%), germacrene D (3.27%) and α-pinene (2.18%) as major constituents. The essential oil and spathulenol exhibited promising cytotoxicity. In vivo tumour growth was inhibited by the treatment with the essential oil (inhibition of 34.46%). Importantly, microencapsulation of the essential oil increased in vivo tumour growth inhibition (inhibition of 62.66%).
Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (CPqGM-FIOCRUZ/BA), Salvador, Brazil.
Larissa M
LM
Bomfim
Department of Chemistry, Federal University of Sergipe, São Cristóvão, Brazil.
Leociley R A
LR
Menezes
Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (CPqGM-FIOCRUZ/BA), Salvador, Brazil.
Ana Carolina B C
AC
Rodrigues
Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (CPqGM-FIOCRUZ/BA), Salvador, Brazil.
Rosane B
RB
Dias
Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (CPqGM-FIOCRUZ/BA), Salvador, Brazil.|Department of Propedeutics, Federal University of Bahia, Salvador, Brazil.
Clarissa A Gurgel
CA
Rocha
Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (CPqGM-FIOCRUZ/BA), Salvador, Brazil.|Center of Biotechnology and Cell Therapy, Hospital São Rafael, Salvador, Brazil.
Milena B P
MB
Soares
Faculty of Pharmacy, Federal University of Bahia, Salvador, Brazil.
Albertino F S
AF
Neto
Northeastern Center for Strategic Technologies (CETENE), Recife, Brazil.
Magaly P
MP
Nascimento
Northeastern Center for Strategic Technologies (CETENE), Recife, Brazil.
Adriana F
AF
Campos
Faculty of Pharmacy, Federal University of Bahia, Salvador, Brazil.
Lidércia C R C E
LC
Silva
Department of Chemistry, Federal University of Amazonas, Manaus, Brazil.
Emmanoel V
EV
Costa
Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (CPqGM-FIOCRUZ/BA), Salvador, Brazil.
Daniel P
DP
Bezerra
D000080462:Acyclic Monoterpenes; D000328:Adult; D000475:Alkenes / Q000494:pharmacology; D000818:Animals; D031041:Annona / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D000081008:Bicyclic Monoterpenes; D045744:Cell Line, Tumor; D000081005:Cyclohexane Monoterpenes; D003512:Cyclohexanones / Q000494:pharmacology; D004195:Disease Models, Animal; D004339:Drug Compounding; D008401:Gas Chromatography-Mass Spectrometry; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D008297:Male; D008546:Melanoma, Experimental / Q000188:drug therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D039821:Monoterpenes / Q000494:pharmacology; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D012717:Sesquiterpenes / Q000494:pharmacology; D045788:Sesquiterpenes, Germacrane / Q000494:pharmacology; D013729:Terpenes / Q000494:pharmacology; D055815:Young Adult; D047392:beta-Cyclodextrins / Q000494:pharmacology
D000080462:Acyclic Monoterpenes; D000475:Alkenes; D000972:Antineoplastic Agents, Phytogenic; D000081008:Bicyclic Monoterpenes; D000081005:Cyclohexane Monoterpenes; D003512:Cyclohexanones; D039821:Monoterpenes; D009822:Oils, Volatile; D012717:Sesquiterpenes; D045788:Sesquiterpenes, Germacrane; D013729:Terpenes; D047392:beta-Cyclodextrins; C443996:beta-ocimene; C005403:alpha phellandrene; C046257:1-methyl-2-isopropylbenzene; C013258:spathulenol; C009729:bicyclohexanone; C005451:alpha-pinene; C027259:germacrene D