Comparison of blood plasma sample preparation methods for combined LC-MS lipidomics and metabolomics.
The goal of this research was to find the most comprehensive lipid extraction of blood plasma, while also providing adequate aqueous preparation for metabolite analysis. Comparisons have been made previously of the Folch, Bligh-Dyer, and Matyash lipid extractions; furthermore, this paper provides an additional comparison of a phospholipid removal plate for analysis. This plate was used for lipid extraction rather than its intended use in lipid removal for polar analysis, and it proves to be robust for targeted lipid analysis. Folch and Matyash provided reproducible recovery over a range of lipid classes, however the Matyash aqueous layer compared well to a typical methanol preparation for polar metabolite analysis. Thus, the Matyash method is the best choice for an untargeted biphasic extraction for metabolomics and lipidomics in blood plasma.
Department of Chemistry, University of Florida, P.O. Box 117200, Gainesville, FL 32611, United States.
Rainey E
RE
Patterson
Department of Chemistry, University of Florida, P.O. Box 117200, Gainesville, FL 32611, United States.
Antoine J
AJ
Ducrocq
Department of Chemistry, University of Florida, P.O. Box 117200, Gainesville, FL 32611, United States.
Danielle J
DJ
McDougall
Biomedical Mass Spectrometry Laboratory, Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL 32610, United States.
Timothy J
TJ
Garrett
Department of Chemistry, University of Florida, P.O. Box 117200, Gainesville, FL 32611, United States; Biomedical Mass Spectrometry Laboratory, Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL 32610, United States. Electronic address: ryost@chem.ufl.edu.
Richard A
RA
Yost
D006801:Humans; D050356:Lipid Metabolism; D055432:Metabolomics; D010949:Plasma / Q000378:metabolism*
LC–MS; Lipidomics; Metabolomics; Sample preparation