L1014F-kdr Mutation in Indian Anopheles subpictus (Diptera: Culicidae) Arising From Two Alternative Transversions in the Voltage-Gated Sodium Channel and a Single PIRA-PCR for Their Detection.
Leucine-to-phenylalanine substitution at residue L1014 in the voltage-gated sodium channel, target site of action for dichlorodiphenyltrichloroethane (DDT) and pyrethroids, is the most common knockdown resistance (kdr) mutation reported in several insects conferring resistance against DDT and pyrethroids. Here, we report presence of two coexisting alternative transversions, A>T and A>C, on the third codon position of L1014 residue in malaria vector Anopheles subpictus Grassi (species A) from Jamshedpur (India), both leading to the same amino acid substitution of Leu-to-Phe with allelic frequencies of 19 and 67%, respectively. A single primer-introduced restriction analysis-polymerase chain reaction (PIRA-PCR) was devised for the identification of L1014F-kdr mutation in An. subpictus resulting from either type of point mutation. Genotyping of samples with PIRA-PCR revealed high frequency (82%) of L1014F-kdr mutation in the study area.
National Institute of Malaria Research (NIMR), Sector-8, Dwarka, New Delhi-110077, India. Singh@mrcindia.org.
O P
OP
Singh
National Institute of Malaria Research (NIMR), Sector-8, Dwarka, New Delhi-110077, India.
C L
CL
Dykes
National Institute of Malaria Research (NIMR), Sector-8, Dwarka, New Delhi-110077, India.
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G
Sharma
National Institute of Malaria Research (ICMR), Field Unit TB Sanatorium Complex, Itki, Ranchi-835301, India.
M K
MK
Das
D019943:Amino Acid Substitution; D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D007194:India; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D061566:Voltage-Gated Sodium Channels / Q000235:genetics / Q000378:metabolism
D019476:Insect Proteins; D061566:Voltage-Gated Sodium Channels
Anopheles subpictus; knockdown resistance; malaria; sodium channel