Porcine circovirus type 2 ORF4 protein binds heavy chain ferritin.
Porcine circovirus type 2 (PCV2) is the primary infectious agent of PCV-associated disease (PCVAD) in swine. ORF4 protein is a newly identified viral protein of PCV2 and is involved in virus-induced apoptosis. However, the molecular mechanisms of ORF4 protein regulation of apoptosis remain unclear, especially given there is no information regarding any cellular partners of the ORF4 protein. Here, we have utilized the yeast two-hybrid assay and identified four host proteins (FHC, SNRPN, COX8A and Lamin C) interacting with the ORF4 protein. Specially, FHC was chosen for further characterization due to its important role in apoptosis. GST pull-down, subcellular co-location and co-immunoprecipitation assays confirmed that the PCV2 ORF4 protein indeed interacted with the heavy-chain ferritin, which is an interesting clue that will allow us to determine the role of the ORF4 protein in apoptosis.
College of Veterinary Medicine, Northwest A and F University, Yangling, Shaanxi 712100, China.
Qizhuang
Q
Lv
Kangkang
K
Guo
Tao
T
Wang
Chengcheng
C
Zhang
Yanming
Y
Zhang
D000818:Animals; D001052:Apoferritins / Q000378:metabolism*; D017209:Apoptosis / Q000235:genetics; D002460:Cell Line; D017925:Circovirus / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D034904:Lamin Type A / Q000378:metabolism; D011485:Protein Binding; D013552:Swine; D013553:Swine Diseases / Q000821:virology; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D055517:snRNP Core Proteins / Q000378:metabolism
D034904:Lamin Type A; D014764:Viral Proteins; C440308:lamin C; D055517:snRNP Core Proteins; D001052:Apoferritins; D003576:Electron Transport Complex IV