New evidence on tick-borne rickettsioses in the Altai region of Russia using primary lesions, serum and blood clots of molecular and serological study.
Tick-borne rickettsioses (TBRs) have similar clinical symptoms and can give serological cross-reaction. We firstly found that in the natural foci of North Asian tick typhus (NATT) in the Altai region of Russia, TBRs can be caused by two Rickettsia species: Rickettsia sibirica subsp. sibirica (causative agent of NATT) and Rickettsia heilongjiangensis. Rickettsial DNA was detected in primary lesions, serum samples and blood clots using real-time PCR. Therefore, each case of TBRs should be verified by using molecular typing. TBR caused by R. sibirica subsp. sibirica - NATT dominates on the territory of Altai region.
Altai State Medical University, Barnaul, 656038, RF, Altai, Barnaul, Lenina str., 40, Russia.
Vladimir
V
Granitov
«N.F. Gamaleya FRCEM», Moscow, Russia. Electronic address: stanislav63@yahoo.com.
Stanislav
S
Shpynov
Altai State Medical University, Barnaul, 656038, RF, Altai, Barnaul, Lenina str., 40, Russia.
Olga
O
Beshlebova
Altai State Medical University, Barnaul, 656038, RF, Altai, Barnaul, Lenina str., 40, Russia.
Irina
I
Arsenjeva
Central Research Institute for Epidemiology (CRIE), Moscow, Russia.
Vladimir
V
Dedkov
Central Research Institute for Epidemiology (CRIE), Moscow, Russia.
Marina
M
Safonova
Central Research Institute for Epidemiology (CRIE), Moscow, Russia.
Olga
O
Stukolova
«N.F. Gamaleya FRCEM», Moscow, Russia.
Anna
A
Pantjukhina
«N.F. Gamaleya FRCEM», Moscow, Russia.
Irina
I
Tarasevich
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D058889:Molecular Typing; D060888:Real-Time Polymerase Chain Reaction; D012281:Rickettsia / Q000145:classification* / Q000235:genetics; D012282:Rickettsia Infections / Q000453:epidemiology* / Q000382:microbiology / Q000473:pathology*; D012426:Russia / Q000453:epidemiology; D017282:Tick-Borne Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000473:pathology; D013987:Ticks / Q000382:microbiology; D055815:Young Adult
D004269:DNA, Bacterial
Altai region; Genotyping; North Asian tick typhus; Rickettsia heilongjiangensis; Rickettsia sibirica subsp. sibirica; Russia