Determination of dopamine in presence of ascorbic acid and uric acid using poly (Spands Reagent) modified carbon paste electrode.
In this paper, we have fabricated a modified carbon paste electrode (CPE) by electropolymerisation of spands reagent (SR) onto surface of CPE using cyclic voltammetry (CV). The developed electrode was abbreviated as poly(SR)/CPE and the surface morphology of the modified electrode was studied by using scanning electron microscopy (SEM). The developed electrode showed higher electrocatalytic properties towards the detection of dopamine (DA) in 0.1M phosphate buffer solution (PBS) at pH7.0. The effect of pH, scan rate, accumulation time and concentration of dopamine was studied at poly(SR)/CPE. The poly(SR)/CPE was successfully used as a sensor for the selective determination of DA in presence of ascorbic acid (AA) and uric acid (UA) without any interference. The poly(SR)/CPE showed a good detection limit of 0.7 μM over the linear dynamic range of 1.6 μM to 16 μM, which is extremely lower than the reported methods. The prepared poly(SR)/CPE exhibited good stability, high sensitivity, better reproducibility, low detection limit towards the determination of DA. The developed method was also applied for the determination of DA in real samples.
Department of Chemistry, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India.
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Veera Manohara Reddy
Department of Chemistry, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India.
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Prabhakara Rao
Department of Chemistry, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India.
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Vijaya Bhaskar Reddy
Department of Chemistry, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India.
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Lavanya
Department of Chemistry, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India.
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Venu
Department of Chemistry, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India.
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Lavanya
Department of Chemistry, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India. Electronic address: gmchem01@gmail.com.
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Madhavi
D001205:Ascorbic Acid / Q000097:blood*; D002244:Carbon / Q000737:chemistry*; D045424:Complex Mixtures / Q000097:blood; D003217:Conductometry / Q000295:instrumentation*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004298:Dopamine / Q000097:blood*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D009824:Ointments; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014527:Uric Acid / Q000097:blood*
D045424:Complex Mixtures; D003432:Cross-Linking Reagents; D009824:Ointments; D014527:Uric Acid; D002244:Carbon; D001205:Ascorbic Acid; D004298:Dopamine
Ascorbic acid; Cyclic voltammetry; Differential pulse voltammetry; Dopamine; Spands reagent