The Inhibitory Mechanism of Gentamicin on Electrical Field Stimulation Response in Rat Bladder Smooth Muscle.
To see the inhibitory mechanism of gentamicin in response to electrical field stimulation (EFS) using the rat bladder smooth muscle, atropine or guanethidine was treated but had no effect. Methylsergide, a non-selective 5-HT1, 5-HT2 receptor antagonist was also treated but had on effect. Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect. For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS. Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor. The inhibition of gentamicin might be mediated through the PLC dependent pathway, but not through the PKC, MLCK or rho kinase dependent pathway.
Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
Chang Ho
CH
Min
Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
YiYi
Y
Wang
Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
Jinhyung
J
Bae
Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
Jung Hoon
JH
Han
Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
Uy Dong
UD
Sohn
EFS; Gentamicin; Phospholipase C; Smooth muscle