Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry.
Mass spectrometry (MS) in Selected Reaction Monitoring (SRM) mode is proposed for in-depth characterisation of microorganisms in a multiplexed analysis. Within 60-80 minutes, the SRM method performs microbial identification (I), antibiotic-resistance detection (R), virulence assessment (V) and it provides epidemiological typing information (T). This SRM application is illustrated by the analysis of the human pathogen Staphylococcus aureus, demonstrating its promise for rapid characterisation of bacteria from positive blood cultures of sepsis patients.
Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.|UMR 5280, Institut des Sciences Analytiques, Université de Lyon, Lyon 1, 5 Rue de la Doua, 69100 Villeurbanne, France.
Yannick
Y
Charretier
Centre National de Référence des Staphylocoques, Centre de Biologie et de Pathologie Est, Hospices Civils de Lyon, Bron, France.|CIRI, International Center for Infectiology Research; INSERM U111; CNRS UMR5308; University of Lyon; Ecole Normale Supérieure de Lyon; Lyon, France.
Olivier
O
Dauwalder
R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
Christine
C
Franceschi
Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
Elodie
E
Degout-Charmette
R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
Gilles
G
Zambardi
Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
Tiphaine
T
Cecchini
Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
Chloe
C
Bardet
R&D ImmunoAssays, Marcy l'Etoile, bioMérieux SA, France.
Xavier
X
Lacoux
R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
Philippe
P
Dufour
Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
Laurent
L
Veron
Technology Research Department, Innovation Unit, Grenoble, bioMérieux SA, France.
Hervé
H
Rostaing
Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
Veronique
V
Lanet
Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
Tanguy
T
Fortin
Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
Corinne
C
Beaulieu
R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
Nadine
N
Perrot
R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
Dominique
D
Dechaume
Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
Sylvie
S
Pons
R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
Victoria
V
Girard
UMR 5280, Institut des Sciences Analytiques, Université de Lyon, Lyon 1, 5 Rue de la Doua, 69100 Villeurbanne, France.
Arnaud
A
Salvador
R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
Géraldine
G
Durand
Technology Research Department, Innovation Unit, Grenoble, bioMérieux SA, France.
Frédéric
F
Mallard
Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
Alain
A
Theretz
Technology Research Department, Innovation Unit, Grenoble, bioMérieux SA, France.
Patrick
P
Broyer
R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
Sonia
S
Chatellier
Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
Gaspard
G
Gervasi
Microbiology Unit, bioMérieux Inc. Durham, NC, USA.
Marc
M
Van Nuenen
Corporate IP Search and Watch, Intellectual Property, Marcy l'Etoile, bioMérieux SA, France.
Carolyn Ann
CA
Roitsch
R&D Microbiologie, La Balme Les Grottes, bioMérieux SA, France.
Alex
A
Van Belkum
UMR 5280, Institut des Sciences Analytiques, Université de Lyon, Lyon 1, 5 Rue de la Doua, 69100 Villeurbanne, France.
Jérôme
J
Lemoine
Centre National de Référence des Staphylocoques, Centre de Biologie et de Pathologie Est, Hospices Civils de Lyon, Bron, France.|CIRI, International Center for Infectiology Research; INSERM U111; CNRS UMR5308; University of Lyon; Ecole Normale Supérieure de Lyon; Lyon, France.
François
F
Vandenesch
Technology Research Department, Innovation Unit, Marcy l'Etoile, bioMérieux SA, France.
Jean-Philippe
JP
Charrier
D001419:Bacteria / Q000145:classification* / Q000187:drug effects* / Q000472:pathogenicity; D015373:Bacterial Typing Techniques / Q000379:methods; D024881:Drug Resistance, Bacterial; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics