Fresh Water Cyanobacteria Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as an Anticancer Drug Resource.
An increasing number of cancer patients worldwide, especially in third world countries, have raised concern to explore natural drug resources, such as the less explored fresh water filamentous cyanobacteria. Six strains of cyanobacteria (Phormidium sp. CCC727, Geitlerinema sp. CCC728, Arthrospira sp. CCC729, Phormidium sp. CCC731, Phormidium sp. CCC730, and Leptolyngbya sp. CCC732) were isolated (paddy fields and ponds in the Banaras Hindu University, campus) and five strains screened for anticancer potential using human colon adenocarcinoma (HT29) and human kidney adenocarcinoma (A498) cancer cell lines. Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 were the most potent as determined by examination of morphological features and by inhibition of growth by graded concentrations of crude extracts and thin-layer chromatography (TLC) eluates. Cell cycle analysis and multiplex assays using cancer biomarkers also confirmed Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as cancer drug resources. Apoptotic studies in the cells of A498 (cancer) and MCF-10A (normal human epithelial) exposed to crude extracts and TLC fractions revealed no significant impact on MCF-10A cells emphasizing its importance in the development of anticancer drug. Identification of biomolecules from these extracts are in progress.
Centre of Advanced Study in Botany, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India.
Akanksha
A
Srivastava
Council Scientific and Industrial Research, Indian Institute of Toxicology Research, Lucknow, 226001, India.
Ratnakar
R
Tiwari
Council Scientific and Industrial Research, Indian Institute of Toxicology Research, Lucknow, 226001, India.
Vikas
V
Srivastava
Division of statistics, Institute of Medical science, Banaras Hindu University, Varanasi, 221005, India.
Tej Bali
TB
Singh
Centre of Advanced Study in Botany, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India.
Ravi Kumar
RK
Asthana
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001685:Biological Factors / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D000458:Cyanobacteria / Q000502:physiology*; D005618:Fresh Water / Q000382:microbiology*; D019073:HT29 Cells; D006801:Humans; D008969:Molecular Sequence Data
D000970:Antineoplastic Agents; D001685:Biological Factors