Endoderm convergence controls subduction of the myocardial precursors during heart-tube formation.
Coordination between the endoderm and adjacent cardiac mesoderm is crucial for heart development. We previously showed that myocardial migration is promoted by convergent movement of the endoderm, which itself is controlled by the S1pr2/Gα13 signaling pathway, but it remains unclear how the movements of the two tissues is coordinated. Here, we image live and fixed embryos to follow these movements, revealing previously unappreciated details of strikingly complex and dynamic associations between the endoderm and myocardial precursors. We found that during segmentation the endoderm underwent three distinct phases of movement relative to the midline: rapid convergence, little convergence and slight expansion. During these periods, the myocardial cells exhibited different stage-dependent migratory modes: co-migration with the endoderm, movement from the dorsal to the ventral side of the endoderm (subduction) and migration independent of endoderm convergence. We also found that defects in S1pr2/Gα13-mediated endodermal convergence affected all three modes of myocardial cell migration, probably due to the disruption of fibronectin assembly around the myocardial cells and consequent disorganization of the myocardial epithelium. Moreover, we found that additional cell types within the anterior lateral plate mesoderm (ALPM) also underwent subduction, and that this movement likewise depended on endoderm convergence. Our study delineates for the first time the details of the intricate interplay between the endoderm and ALPM during embryogenesis, highlighting why endoderm movement is essential for heart development, and thus potential underpinnings of congenital heart disease.
Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, 1-400 Bowen Science Building, 51 Newton Road, Iowa City, IA 52242-1109, USA.
Ding
D
Ye
Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, 1-400 Bowen Science Building, 51 Newton Road, Iowa City, IA 52242-1109, USA.
Huaping
H
Xie
Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, 1-400 Bowen Science Building, 51 Newton Road, Iowa City, IA 52242-1109, USA.
Bo
B
Hu
Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, 1-400 Bowen Science Building, 51 Newton Road, Iowa City, IA 52242-1109, USA fang-lin@uiowa.edu.
Fang
F
Lin
D000818:Animals; D019521:Body Patterning; D002465:Cell Movement; D004707:Endoderm / Q000166:cytology / Q000196:embryology*; D004848:Epithelium / Q000196:embryology / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D044365:GTP-Binding Protein alpha Subunits, G12-G13 / Q000378:metabolism; D006321:Heart / Q000196:embryology*; D008648:Mesoderm / Q000166:cytology / Q000196:embryology; D009206:Myocardium / Q000166:cytology*; D038081:Organogenesis; D049349:Receptors, Lysosphingolipid / Q000378:metabolism; D015398:Signal Transduction; D015027:Zebrafish / Q000196:embryology / Q000378:metabolism; D029961:Zebrafish Proteins / Q000378:metabolism
D005353:Fibronectins; D049349:Receptors, Lysosphingolipid; D029961:Zebrafish Proteins; D044365:GTP-Binding Protein alpha Subunits, G12-G13
Endoderm convergence; In vivo imaging; Myocardial migration; S1pr2/Gα13; Subduction