CLA and CD62E expression in oral lichen planus lesions.
OBJECTIVE
There are few reports on the migration of CLA+ T cells through E-selectin in cutaneous lichen planus, with only one study on oral lichen planus (OLP). This study aimed to analyze CLA expression and assess whether there is a correlation with E-selectin (CD62E) in OLP lesions.


METHODS
Biopsies were performed on 11 patients including two areas: one without clinical and histopathological features of OLP [perilesional group (PLG)] and the other with clinical and histopathological features of OLP [OLP group (OLPG)]. The specimens obtained were divided into two: One was fixed in formalin for routine analysis (H&E), and the other was frozen for CD3, CD4, CD8, CLA, and CD62E immunofluorescence markers.


RESULTS
More CD4+ (median 1409, range 860-2519), CD8+ (median 1568, range 654-3258), and CLA+ T cells (median 958, range 453-2198) and higher CD62E expression (median 37, range 27-85) were identified in OLPG (P = 0.003; P = 0.003; P = 0.004; P = 0.003, respectively) than those in PLG. The median prevalence analysis was also significantly higher for CLA+CD8+ T cells in OLPG (OLPG = 39.4%, range 18.4-64.2; PLG = 29.4%, range 12.1-47.1) (P = 0.026). None of the correlations between CD3+ or CLA+ T cells and CD62E in OLPG and in PLG were significant.


CONCLUSIONS
The significant presence of CLA+ T cells and E-selectin expressions in the OLPG suggests their involvement in the etiopathogenesis of OLP; however, only a weak correlation between CLA+ T cells and E-selectin was observed.
Medical School, Fluminense Federal University, Rio de Janeiro, Brazil.
Juliana Tristão
JT
Werneck
Medical School, Fluminense Federal University, Rio de Janeiro, Brazil.
Eliane Pedra
EP
Dias
Dental School, Estácio de Sá University, Rio de Janeiro, Brazil.
Lucio Souza
LS
Gonçalves
Medical School, Fluminense Federal University, Rio de Janeiro, Brazil.
Arley
A
Silva Junior
D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D019040:E-Selectin / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods; D006801:Humans; D008010:Lichen Planus / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D017676:Lichen Planus, Oral / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D009061:Mouth Mucosa / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D015995:Prevalence; D013601:T-Lymphocytes / Q000276:immunology
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; C063419:CTAGE1 protein, human; D019040:E-Selectin; D008562:Membrane Glycoproteins; C546936:SELE protein, human
CD8; CLA; E-selectin; Oral lichen planus; lymphocytes; oral immunology