The Role of Bone Marrow Cells in the Phenotypic Changes Associated with Diabetic Nephropathy.
The aim of our study was to investigate the role of bone marrow cells in the phenotypic changes that occur in diabetic nephropathy. Bone marrow cells were obtained from either streptozotocin-induced diabetic or untreated control C3H/He mice and transplanted into control C3H/He mice. Eight weeks after bone marrow cell transplantation, renal morphologic changes and clinical parameters of diabetic nephropathy, including the urine albumin/creatinine ratio and glucose tolerance, were measured in vivo. Expression levels of the genes encoding α1 type IV collagen and transforming growth factor-β1 in the kidney were assayed. Our results demonstrated that glucose tolerance was normal in the recipients of bone marrow transplants from both diabetic and control donors. However, compared with recipients of the control bone marrow transplant, the urinary albumin/creatinine ratio, glomerular size, and the mesangial/glomerular area ratio increased 3.3-fold (p < 0.01), 1.23-fold (p < 0.01), and 2.13-fold (p < 0.001), respectively, in the recipients of the diabetic bone marrow transplant. Expression levels of the genes encoding glomerular α1 type IV collagen and transforming growth factor-β1 were also significantly increased (p < 0.01) in the recipients of the diabetic bone marrow transplant. Our data suggest that bone marrow cells from the STZ-induced diabetic mice can confer a diabetic phenotype to recipient control mice without the presence of hyperglycemia.
Department of Geriatric Nephrology, Chinese People's Liberation Army General Hospital, State Key Laboratory of Kidney Disease, Beijing, China.
Guang
G
Yang
Department of Geriatric Nephrology, Chinese People's Liberation Army General Hospital, State Key Laboratory of Kidney Disease, Beijing, China.
Qingli
Q
Cheng
Department of Geriatric Nephrology, Chinese People's Liberation Army General Hospital, State Key Laboratory of Kidney Disease, Beijing, China.
Sheng
S
Liu
Department of Geriatric Nephrology, Chinese People's Liberation Army General Hospital, State Key Laboratory of Kidney Disease, Beijing, China.
Jiahui
J
Zhao
D000419:Albuminuria / Q000473:pathology / Q000652:urine; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology*; D016026:Bone Marrow Transplantation; D024141:Collagen Type IV / Q000235:genetics / Q000378:metabolism; D003404:Creatinine / Q000652:urine; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D003928:Diabetic Nephropathies / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005786:Gene Expression Regulation; D005920:Glomerular Mesangium / Q000378:metabolism / Q000473:pathology*; D005951:Glucose Tolerance Test; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D013311:Streptozocin; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
D001786:Blood Glucose; D024141:Collagen Type IV; D053773:Transforming Growth Factor beta1; D013311:Streptozocin; D003404:Creatinine