Bmp2 conditional knockout in osteoblasts and endothelial cells does not impair bone formation after injury or mechanical loading in adult mice.
Post-natal osteogenesis after mechanical trauma or stimulus occurs through either endochondral healing, intramembranous healing or lamellar bone formation. Bone morphogenetic protein 2 (BMP2) is up-regulated in each of these osteogenic processes and is expressed by a variety of cells including osteoblasts and vascular cells. It is known that genetic knockout of Bmp2 in all cells or in osteo-chondroprogenitor cells completely abrogates endochondral healing after full fracture. However, the importance of BMP2 from differentiated osteoblasts and endothelial cells is not known. Moreover, the importance of BMP2 in non-endochondral bone formation such as intramembranous healing or lamellar bone formation is not known. Using inducible and tissue-specific Cre-lox mediated targeting of Bmp2 in adult (10-24 week old) mice, we assessed the role of BMP2 expression globally, by osteoblasts, and by vascular endothelial cells in endochondral healing, intramembranous healing and lamellar bone formation. These three osteogenic processes were modeled using full femur fracture, ulnar stress fracture, and ulnar non-damaging cyclic loading, respectively. Our results confirmed the requirement of BMP2 for endochondral fracture healing, as mice in which Bmp2 was knocked out in all cells prior to fracture failed to form a callus. Targeted deletion of Bmp2 in osteoblasts (osterix-expressing) or vascular endothelial cells (vascular endothelial cadherin-expressing) did not impact fracture healing in any way. Regarding non-endochondral bone formation, we found that BMP2 is largely dispensable for intramembranous bone formation after stress fracture and also not required for lamellar bone formation induced by mechanical loading. Taken together our results indicate that osteoblasts and endothelial cells are not a critical source of BMP2 in endochondral fracture healing, and that non-endochondral bone formation in the adult mouse is not as critically dependent on BMP2.
Department of Orthopaedic Surgery, Saint Louis University School of Medicine, 1402 S. Grand Blvd, Schwitalla Hall, M176, St. Louis, MO 63104, USA; Department of Orthopedic Surgery, Washington University in St. Louis School of Medicine, 660 S. Euclid, Campus Box8233, St. Louis, MO 63110, USA. Electronic address: SMcBrid9@slu.edu.
Sarah Howe
SH
McBride-Gagyi
Department of Orthopedic Surgery, Washington University in St. Louis School of Medicine, 660 S. Euclid, Campus Box8233, St. Louis, MO 63110, USA. Electronic address: McKenzieJ@wudosis.wustl.edu.
Jennifer A
JA
McKenzie
Department of Orthopedic Surgery, Washington University in St. Louis School of Medicine, 660 S. Euclid, Campus Box8233, St. Louis, MO 63110, USA. Electronic address: BuettmannE@wustl.edu.
Evan G
EG
Buettmann
Department of Orthopedic Surgery, Washington University in St. Louis School of Medicine, 660 S. Euclid, Campus Box8233, St. Louis, MO 63110, USA. Electronic address: GardnerM@wudosis.wustl.edu.
Michael J
MJ
Gardner
Department of Orthopedic Surgery, Washington University in St. Louis School of Medicine, 660 S. Euclid, Campus Box8233, St. Louis, MO 63110, USA. Electronic address: SilvaM@wudosis.wustl.edu.
Matthew J
MJ
Silva
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000172:deficiency* / Q000235:genetics / Q000502:physiology; D042783:Endothelial Cells / Q000502:physiology; D005260:Female; D017102:Fracture Healing / Q000235:genetics / Q000502:physiology*; D015775:Fractures, Stress / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D015870:Gene Expression; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology*; D013314:Stress, Mechanical
C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2
BMP2; Bone homeostasis; Bone injury; Facture healing; Orthopedics; Osteoblasts