Functions of OsDof25 in regulation of OsC4PPDK.
Relative little is known about the functions of the so-called Dof zinc factors in plants. Here we report on the analysis of OsDof25 and show a function in regulation of the important C4 photosynthesis gene, OsC4PPDK in rice. Over-expression of OsDof25 enhanced the expression of OsC4PPDK in transient expression experiments by binding in a specific way to a conserved Dof binding site which was confirmed by yeast and in vitro binding studies. Expression studies using promoter GUS plants as well as qPCR experiments showed that OsDof25 expressed in different tissues including both photosynthetic and non-photosynthetic organs and that expression of OsDof25 was partially overlapping with the OsC4PPDK gene. Conclusive evidence for a role of OsDof25 in regulation of C4PPDK came from loss-of-function and gain-of-function experiments with transgenic rice, which showed that down-regulation or over-expression of OsDof25 correlated with OsC4PPDK expression and that OsDof25 has functions as transcriptional activator.
Department of Molecular and Developmental Genetics, Institute of Biology (IBL), Leiden University, P.O. Box 9505, 2300 RA, Leiden, The Netherlands.|State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, Beijing, 100101, China.|Graduate School of the Chinese Academy of Sciences, Beijing, 100049, China.
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Zhang
Department of Molecular and Developmental Genetics, Institute of Biology (IBL), Leiden University, P.O. Box 9505, 2300 RA, Leiden, The Netherlands.
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Verhoeff
Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Wusi Rd 247, Fuzhou, 350003, Fujian, China.
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Chen
Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Wusi Rd 247, Fuzhou, 350003, Fujian, China.
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Chen
Department of Molecular and Developmental Genetics, Institute of Biology (IBL), Leiden University, P.O. Box 9505, 2300 RA, Leiden, The Netherlands.|SU BioMedicine/TNO Quality of Life, Zernikedreef 9, P.O. Box 2215, 2301 CE, Leiden, The Netherlands.
Mei
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Wang
State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, Beijing, 100101, China.
Zhen
Z
Zhu
Department of Molecular and Developmental Genetics, Institute of Biology (IBL), Leiden University, P.O. Box 9505, 2300 RA, Leiden, The Netherlands. p.b.f.ouwerkerk.2@gmail.com.
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Ouwerkerk
D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D005810:Multigene Family; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D018515:Plant Leaves; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018547:Plant Stems; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D036226:Seedlings; D015533:Transcriptional Activation; D059467:Transcriptome
D018744:DNA, Plant; D010940:Plant Proteins
Dof; OsC4PPDK; Rice; Transcription factor