Metabolic profiling as a tool for prioritizing antimicrobial compounds.
Metabolomics is an analytical technique that allows scientists to globally profile low molecular weight metabolites between samples in a medium- or high-throughput environment. Different biological samples are statistically analyzed and correlated to a bioactivity of interest, highlighting differentially produced compounds as potential biomarkers. Here, we review NMR- and MS-based metabolomics as technologies to facilitate the identification of novel antimicrobial natural products from microbial sources. Approaches to elicit the production of poorly expressed (cryptic) molecules are thereby a key to allow statistical analysis of samples to identify bioactive markers, while connection of compounds to their biosynthetic gene cluster is a determining step in elucidating the biosynthetic pathway and allows downstream process optimization and upscaling. The review focuses on approaches built around NMR-based metabolomics, which enables efficient dereplication and guided fractionation of (antimicrobial) compounds.
Molecular Biotechnology, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE, Leiden, The Netherlands.|Natural Products Laboratory, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE, Leiden, The Netherlands.
Changsheng
C
Wu
Natural Products Laboratory, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE, Leiden, The Netherlands.
Young Hae
YH
Choi
Molecular Biotechnology, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE, Leiden, The Netherlands. g.wezel@chem.leidenuniv.nl.|Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, The Netherlands. g.wezel@chem.leidenuniv.nl.
Gilles P
GP
van Wezel
D000890:Anti-Infective Agents / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D001688:Biological Products / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D004352:Drug Resistance, Microbial; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D055432:Metabolomics; D005810:Multigene Family
D000890:Anti-Infective Agents; D001688:Biological Products; D015415:Biomarkers
Antibiotic production; Antimicrobial drug resistance; Metabolomics; NMR; Streptomyces