The detection and genetic characterization based on the S1 gene region of BCoVs from respiratory and enteric infections in Turkey.
We investigated bovine coronavirus (BCoV) as an etiological agent in cattle with clinical respiratory and digestive signs using 147 feces and 199 nasal swab samples. A total of 18 test samples (16 feces and 2 nasal swap samples) were detected positive by ELISA and/or RT-PCR targeting the BCoV N gene. The partial S1 gene regions of BCoVs (An-4 and An-11) detected in feces samples from two herd-mate dairy calves were compared. Virological and serological results indicated that BCoVs are widespread in Turkey and are likely etiological agents in diarrhea cases in calves.
Faculty of Veterinary Medicine, Department of Virology Diskapi, Ankara University, Ankara, Turkey.  falkan@veterinary.ankara.edu.tr
F
F
Alkan
Faculty of Veterinary Medicine, Department of Virology Diskapi, Ankara University, Ankara, Turkey  Ministry of Agriculture and Rural Affairs, Central Veterinary Control and Research Institute, Etlik, Ankara, Turkey.
A
A
Ozkul
Faculty of Veterinary Medicine, Department of Virology Diskapi, Ankara University, Ankara, Turkey  Ministry of Agriculture and Rural Affairs, Central Veterinary Control and Research Institute, Etlik, Ankara, Turkey.
S
S
Bilge-Dagalp
Faculty of Veterinary Medicine, Department of Virology Diskapi, Ankara University, Ankara, Turkey  Ministry of Agriculture and Rural Affairs, Central Veterinary Control and Research Institute, Etlik, Ankara, Turkey.
T
T
Karaoglu
Faculty of Veterinary Medicine, Department of Virology Diskapi, Ankara University, Ankara, Turkey  Ministry of Agriculture and Rural Affairs, Central Veterinary Control and Research Institute, Etlik, Ankara, Turkey.
T C
TC
Oguzoglu
Faculty of Veterinary Medicine, Department of Virology Diskapi, Ankara University, Ankara, Turkey  Ministry of Agriculture and Rural Affairs, Central Veterinary Control and Research Institute, Etlik, Ankara, Turkey.
E
E
Caliskan
Faculty of Veterinary Medicine, Department of Virology Diskapi, Ankara University, Ankara, Turkey  Ministry of Agriculture and Rural Affairs, Central Veterinary Control and Research Institute, Etlik, Ankara, Turkey.
I
I
Burgu
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017938:Coronavirus, Bovine / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000821:virology; D008969:Molecular Sequence Data; D009666:Nose / Q000821:virology; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000662:veterinary; D064370:Spike Glycoprotein, Coronavirus / Q000235:genetics* / Q000378:metabolism; D014421:Turkey / Q000453:epidemiology
D064370:Spike Glycoprotein, Coronavirus
S1 gene region; bovine; coronavirus; nested RT-PCR; sequencing