Role of miR-511 in the Regulation of OATP1B1 Expression by Free Fatty Acid.
MicroRNAs (miRNAs) are a family of non-coding RNA that are able to adjust the expression of many proteins, including ATP-binding cassette transporter and organic cation transporter. We sought to evaluate the effect of miR-511 on the regulation of OATP1B1 expression by free fatty acids. When using free fatty acids to stimulate Chang liver cells, we found that the expression of miR-511 increased significantly while the expression of OATP1B1 decreased. We also proved that SLCO1B1 is the target gene of miR-511 with a bioinformatics analysis and using the dual luciferase reporter assay. Furthermore, the expressions of SLCO1B1 and OATP1B1 decreased if transfecting Chang liver cells with miR-511, but did not increase when transfecting the inhibitors of miR-511 into steatosis cells. Our study indicates that miR-511 may play an important role in the regulation of OATP1B1 expression by free fatty acids.
Department of Pharmacy, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410000 ; Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013.
Jin Fu
JF
Peng
Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013.
Li
L
Liu
Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013.
Cheng Xian
CX
Guo
Department of Pharmacy, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410000.
Shi Kun
SK
Liu
Institute of Clinical Pharmacology, Central South University, Changsha, Hunan 410013, China.
Xiao Ping
XP
Chen
Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013.
Li Hua
LH
Huang
Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013.
Hong
H
Xiang
Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013.
Zhi Jun
ZJ
Huang
Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013.
Hong
H
Yuan
Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013.
Guo Ping
GP
Yang
Expression; Free fatty acid; OATP1B1; SCLO1B1; miR-511