A biomimetic collagen/heparin multi-layered porous hydroxyapatite orbital implant for in vivo vascularization studies on the chicken chorioallantoic membrane.
BACKGROUND
The vascularization of an orbital implant is a key issue for reducing complications, such as exposure and infection.


METHODS
Here, we developed a facile layer-by-layer assembly approach to modify porous hydroxyapatite (pHA) orbital implants with five collagen (COL)/heparin (HEP) multilayers.


RESULTS
SEM characterization showed that the average pore size of the pHA/(COL/HEP)5 scaffold was 316.8 ± 77.1 μm. After being coated with five COL/HEP multilayers, the mechanical strength was improved compared with that of the pHA scaffolds. The in vitro assay displayed that the pHA scaffolds covered with COL/HEP multilayers resulted in a larger number of human umbilical vein endothelial cells after being cultured for 14 days. The macroscopic evaluation and semi-quantitative vascular density analysis of the chicken chorioallantoic membrane assay showed that the pHA/(COL/HEP)5 scaffolds resulted in more intense angiogenesis than the pHA scaffolds.


CONCLUSIONS
These studies demonstrate that the biomembrane-mimicking coating of COL/HEP multilayers is a simple and effective strategy to endow combined biological performances of pHA orbital implants and to potentially reduce implant-related complications.
Department of Ophthalmology, the Second Affiliated Hospital of Zhejiang University, College of Medicine, Hangzhou, 310009, China.
Kai
K
Jin
Department of Ophthalmology, the Second Affiliated Hospital of Zhejiang University, College of Medicine, Hangzhou, 310009, China.
Xin
X
Ye
Key Laboratory of Molecular Animal Nutrition of the Ministry of Education and Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Sha
S
Li
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
Bo
B
Li
Key Laboratory of Molecular Animal Nutrition of the Ministry of Education and Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Caiqiao
C
Zhang
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China. cygao@mail.hz.zj.cn.
Changyou
C
Gao
Department of Ophthalmology, the Second Affiliated Hospital of Zhejiang University, College of Medicine, Hangzhou, 310009, China. yejuan@zju.edu.cn.
Juan
J
Ye
D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D002470:Cell Survival; D002478:Cells, Cultured; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000098:blood supply*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D024042:Collagen Type I / Q000737:chemistry*; D017886:Durapatite; D006493:Heparin / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic; D020273:Orbital Implants; D016062:Porosity; D054457:Tissue Scaffolds
D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D006493:Heparin; D017886:Durapatite
Heparin; Hydroxyapatite; Layer-by-layer assembly; Orbital implant; Vascularization