Quantitative proteomic analysis of Vietnamese krait venoms: Neurotoxins are the major components in Bungarus multicinctus and phospholipases A2 in Bungarus fasciatus.
Kraits are venomous snakes of genus Bungarus from family Elapidae. Krait venoms are generally neurotoxic, but toxicity strongly depends on the particular species and regional origin of snakes. We analyzed the proteomes of Vietnamese Bungarus multicinctus and Bungarus fasciatus venoms both qualitatively and quantitatively. It should be noted that no proteomic data for B. multicinctus venom existed so far. We have found that in this venom, almost half (45%) of the proteins by weight was represented by β-bungarotoxins, followed by three finger toxins (28%) and phospholipases A2 (16%), other proteins being present at the level of 1-3%. In B. fasciatus venom, phospholipase A2 was the main component (71%), followed by oxidase of l-amino acids (8%), acetylcholinesterase (5%) and metalloproteinases (4%). Unexpectedly, extremely low amount of three finger toxins (1%) was found in this venom. Interestingly, the presence of complement depleting factor was observed in both venoms. Although our data showed the presence of the same toxin families in Vietnamese krait venoms as those found earlier in the venoms of kraits from other geographic regions, their relative ratio is completely different. This concerns especially B. fasciatus venom with predominant content of phospholipases A2 and very low amount of three finger toxins.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.
Rustam H
RH
Ziganshin
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.
Sergey I
SI
Kovalchuk
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.
Georgij P
GP
Arapidi
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.
Vladislav G
VG
Starkov
Institute of Applied Materials Science, Vietnam Academy of Science and Technology, 01 Mac Dinh Chi Street, District 1, Ho Chi Minh, Viet Nam.
Anh Ngoc
AN
Hoang
Institute of Applied Materials Science, Vietnam Academy of Science and Technology, 01 Mac Dinh Chi Street, District 1, Ho Chi Minh, Viet Nam.
Thao Thanh
TT
Thi Nguyen
Institute of Applied Materials Science, Vietnam Academy of Science and Technology, 01 Mac Dinh Chi Street, District 1, Ho Chi Minh, Viet Nam.
Khoa Cuu
KC
Nguyen
Anokhin Research Institute of Normal Physiology, Ul. Baltiyskaya 8, 125315, Moscow, Russia.
Batozhab B
BB
Shoibonov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.
Victor I
VI
Tsetlin
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia; The National University of Science and Technology MISiS, Leninskiy Prospekt 2, 119049, Moscow, Russia. Electronic address: utkin@mx.ibch.ru.
Yuri N
YN
Utkin
D000818:Animals; D017838:Bungarus; D002853:Chromatography, Liquid; D004546:Elapid Venoms / Q000737:chemistry*; D020224:Expressed Sequence Tags; D013058:Mass Spectrometry; D009498:Neurotoxins / Q000032:analysis / Q000737:chemistry*; D054467:Phospholipases A2 / Q000032:analysis / Q000737:chemistry*; D040901:Proteomics; D030162:Reptilian Proteins / Q000032:analysis / Q000737:chemistry*
D004546:Elapid Venoms; D009498:Neurotoxins; D030162:Reptilian Proteins; D054467:Phospholipases A2
Bungarus fasciatus; Bungarus multicinctus; Krait; Mass-spectrometry; Proteome; Snake venom