Evaluation of the Wound Healing Properties of Hancornia speciosa Leaves.
The leaves of Hancornia speciosa Gomes (Apocynaceae), a medicinal species found in the Brazilian cerrado biome, are traditionally used to treat wounds and inflammatory disorders. The goal of the present study was to investigate the in vitro wound healing properties of ethanolic extract of H. speciosa leaves and its isolated compounds, using the scratch assay, and to evaluate their effects on the release of the pro-inflammatory cytokine tumor necrosis factor (TNF-α) by lipopolysaccharide (LPS)-stimulated human acute monocytic (THP-1) cells. H. speciosa ethanolic extract significantly increased (42.8% ± 5.4 at 25 µg/mL) cell migration and proliferation of fibroblasts compared with control cells, as well as the isolated compounds bornesitol (80.8% ± 5.1) and quinic acid (69.1% ± 6.2), both assayed at 50 μM. TNF-α release by LPS-stimulated THP-1 cells was significantly reduced by the ethanolic extract (62.9% ± 8.2, i.e. 1791.1 ± 394.7 pg/mL) at 10 µg/mL, bornesitol (48.9% ± 0.9, i.e. 2461.6 ± 43.1 pg/mL) at 50 μM, and quinic acid (90.2% ± 3.4, i.e. 473.5 ± 164.4 pg/mL) and rutin (82.4% ± 5.6, i.e. 847.0 ± 271.8 pg/mL) at 10 μM. These results provided evidences to support the traditional use of H. speciosa leaves to treat wounds and inflammatory disorders.
Department of Pharmaceutical Sciences, Center of Health Sciences, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, SC, 88.040-900, Brazil.
Fabiana Cristina
FC
Geller
Department of Pharmaceutical Sciences, Center of Health Sciences, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, SC, 88.040-900, Brazil.
Marina Rodrigues
MR
Teixeira
Department of Pharmaceutical Products, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Ana Bárbara Dias
AB
Pereira
Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Campus Pampulha, Belo Horizonte, MG, 31.270-901, Brazil.
Luana Pereira Antunes
LP
Dourado
Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Campus Pampulha, Belo Horizonte, MG, 31.270-901, Brazil.
Danielle G
DG
Souza
Department of Pharmaceutical Products, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Fernão Castro
FC
Braga
Department of Pharmaceutical Sciences, Center of Health Sciences, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, SC, 88.040-900, Brazil.
Cláudia Maria Oliveira
CM
Simões
D029062:Apocynaceae / Q000737:chemistry*; D001938:Brazil; D002460:Cell Line; D054812:Cyclitols / Q000302:isolation & purification / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D008070:Lipopolysaccharides; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D011801:Quinic Acid / Q000302:isolation & purification / Q000494:pharmacology; D012431:Rutin / Q000302:isolation & purification / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
D054812:Cyclitols; C546633:L-bornesitol; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D011801:Quinic Acid; D012431:Rutin
Hancornia speciosa; TNF-α; bornesitol; quinic acid; scratch assay; wound healing