Square Wave Voltammetric Determination of Diclofenac in Pharmaceutical Preparations and Human Serum.
In this study, a simple and reliable square wave voltammetric (SWV) method was developed and validated for determination of diclofenac in pharmaceutical preparations and human serum. The proposed method was based on electrooxidation of diclofenac at platinum electrode in 0.1 M TBAClO4/acetonitrile solution. The well-defined two oxidation peaks were observed at 0.87 and 1.27 V, respectively. Calibration curves that were obtained by using current values measured for second peak were linear over the concentration range of 1.5-17.5 μg mL(-1) and 2-20 μg mL(-1) in supporting electrolyte and serum, respectively. Precision and accuracy were also checked in all media. Intra- and inter-day precision values for diclofenac were less than 3.64, and accuracy (relative error) was better than 2.49%. Developed method in this study is accurate, precise and can be easily applied to Diclomec, Dicloflam and Voltaren tablets as pharmaceutical preparation. Also, the proposed technique was successfully applied to spiked human serum samples. No electroactive interferences from the endogenous substances were found in human serum.
Department of Analytical Chemistry, School of Pharmacy, Ataturk University, Erzurum, Turkey.
Ulvihan
U
Ciltas
Department of Analytical Chemistry, School of Pharmacy, Ataturk University, Erzurum, Turkey.
Bilal
B
Yilmaz
Department of Analytical Chemistry, School of Pharmacy, Ataturk University, Erzurum, Turkey.
Selcuk
S
Kaban
Department of Analytical Chemistry, School of Pharmacy, Ataturk University, Erzurum, Turkey.
Bilge Kaan
BK
Akcay
Department of Analytical Chemistry, School of Pharmacy, Ataturk University, Erzurum, Turkey.
Gulsah
G
Nazik
Cyclic voltammetry; Diclofenac; Pharmaceutical tablet; Serum; Square wave voltammetry