Highly specific detection of genetic modification events using an enzyme-linked probe hybridization chip.
The enzyme-linked probe hybridization chip utilizes a method based on ligase-hybridizing probe chip technology, with the principle of using thio-primers for protection against enzyme digestion, and using lambda DNA exonuclease to cut multiple PCR products obtained from the sample being tested into single-strand chains for hybridization. The 5'-end amino-labeled probe was fixed onto the aldehyde chip, and hybridized with the single-stranded PCR product, followed by addition of a fluorescent-modified probe that was then enzymatically linked with the adjacent, substrate-bound probe in order to achieve highly specific, parallel, and high-throughput detection. Specificity and sensitivity testing demonstrated that enzyme-linked probe hybridization technology could be applied to the specific detection of eight genetic modification events at the same time, with a sensitivity reaching 0.1% and the achievement of accurate, efficient, and stable results.
Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, China.
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MZ
Zhang
Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, China.
X F
XF
Zhang
Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, China.
X M
XM
Chen
Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, China.
X
X
Chen
Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, China.
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S
Wu
Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, China.
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LL
Xu
D014644:Genetic Variation; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity