Noncoding RNAs: Possible Players in the Development of Fluorosis.
Fluorosis is caused by excess of fluoride intake over a long period of time. Aberrant change in the Runt-related transcription factor 2 (RUNX2) mediated signaling cascade is one of the decisive steps during the pathogenesis of fluorosis. Up to date, role of fluoride on the epigenetic alterations is not studied. In the present study, global expression profiling of short noncoding RNAs, in particular miRNAs and snoRNAs, was carried out in sodium fluoride (NaF) treated human osteosarcoma (HOS) cells to understand their possible role in the development of fluorosis. qPCR and in silico hybridization revealed that miR-124 and miR-155 can be directly involved in the transcriptional regulation of Runt-related transcription factor 2 (RUNX2) and receptor activator of nuclear factor κ-B ligand (RANKL) genes. Compared to control, C/D box analysis revealed marked elevation in the number of UG dinucleotides and D-box sequences in NaF exposed HOS cells. Herein, we report miR-124 and miR-155 as the new possible players involved in the development of fluorosis. We show that the alterations in UG dinucleotides and D-box sequences of snoRNAs could be due to NaF exposure.
Environmental Health Division, National Environmental Engineering Research Institute (NEERI), CSIR, Nagpur 440020, India.
Atul P
AP
Daiwile
Environmental Health Division, National Environmental Engineering Research Institute (NEERI), CSIR, Nagpur 440020, India.
Saravanadevi
S
Sivanesan
Department of Health Sciences, University of Genoa, No. 5, 16126 Genoa, Italy ; IRCCS AOU San Martino IST, No. 10, 16132 Genoa, Italy.
Alberto
A
Izzotti
Environmental Health Division, National Environmental Engineering Research Institute (NEERI), CSIR, Nagpur 440020, India.
Amit
A
Bafana
Environmental Health Division, National Environmental Engineering Research Institute (NEERI), CSIR, Nagpur 440020, India.
Pravin K
PK
Naoghare
CNR Institute for Macromolecular Studies, No. 6, 16149 Genoa, Italy.
Patrizio
P
Arrigo
Environmental Genomics Division, National Environmental Engineering Research Institute (NEERI), CSIR, Nagpur 440020, India.
Hemant J
HJ
Purohit
Developmental Toxicology, Indian Institute of Toxicology Research, Lucknow 226001, India.
Devendra
D
Parmar
Environmental Health Division, National Environmental Engineering Research Institute (NEERI), CSIR, Nagpur 440020, India.
Krishnamurthi
K
Kannan
D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D050659:Core Binding Factor Alpha 1 Subunit / Q000096:biosynthesis* / Q000235:genetics; D009050:Fluorosis, Dental / Q000235:genetics* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D012516:Osteosarcoma / Q000150:complications / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D053245:RANK Ligand / Q000096:biosynthesis* / Q000235:genetics; D020537:RNA, Small Nucleolar / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D012969:Sodium Fluoride / Q000633:toxicity
D050659:Core Binding Factor Alpha 1 Subunit; C509207:MIRN124 microRNA, human; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D053245:RANK Ligand; D020537:RNA, Small Nucleolar; C493709:RUNX2 protein, human; C505688:TNFSF11 protein, human; D012969:Sodium Fluoride