NETosis in Neonates: Evidence of a Reactive Oxygen Species-Independent Pathway in Response to Fungal Challenge.
Release of neutrophil extracellular traps (NETs) is a significant antimicrobial host defense mechanism in adults. In neonates, fungal sepsis is a frequent cause of morbidity and mortality and may be a consequence of inadequate neutrophil defense functions. Like neutrophils from adult donors, we found that neutrophils from neonates formed robust cellular aggregates and released NETs in response to fungal β-glucan and Candida albicans hyphae when presented with extracellular matrix. Therefore, in response to fungal stimulation, neonatal neutrophils are capable of NETosis. Neonate susceptibility to fungal infections may not be due to an inability of their neutrophils to produce NETs.
Division of Surgical Research, Department of Surgery, Rhode Island Hospital Warren Alpert Medical School, Brown University Graduate Program in Pathobiology, Brown University Department of Pediatrics, Women and Infants Hospital of Rhode Island, Providence.
Angel S
AS
Byrd
Division of Surgical Research, Department of Surgery, Rhode Island Hospital.
Xian M
XM
O'Brien
Department of Pediatrics, Women and Infants Hospital of Rhode Island, Providence.
Sonia S
SS
Laforce-Nesbitt
Division of Surgical Research, Department of Surgery, Rhode Island Hospital.
Valentina E
VE
Parisi
Warren Alpert Medical School, Brown University Graduate Program in Pathobiology, Brown University.
Matthew P
MP
Hirakawa
Warren Alpert Medical School, Brown University Graduate Program in Pathobiology, Brown University Department of Pediatrics, Women and Infants Hospital of Rhode Island, Providence.
Joseph M
JM
Bliss
Division of Surgical Research, Department of Surgery, Rhode Island Hospital Warren Alpert Medical School, Brown University Graduate Program in Pathobiology, Brown University.
Jonathan S
JS
Reichner
D000328:Adult; D002176:Candida albicans / Q000276:immunology*; D065206:Extracellular Traps / Q000378:metabolism*; D006801:Humans; D025301:Hyphae / Q000276:immunology; D007231:Infant, Newborn; D009504:Neutrophils / Q000276:immunology*; D047071:beta-Glucans / Q000276:immunology
D047071:beta-Glucans
NETs; fungi; glucan; neonates; neutrophils