Self-aligning subatmospheric hybrid liquid junction electrospray interface for capillary electrophoresis.
We report a construction of a self-aligning subatmospheric hybrid liquid junction electrospray interface for CE eliminating the need for manual adjustment by guiding the capillaries in a microfabricated liquid junction glass chip at a defined angle. Both the ESI and separation capillaries are inserted into the microfabricated part until their ends touch. The distance between the capillary openings is defined by the angle between the capillaries. The microfabricated part contains channels for placement of the capillaries and connection of the external electrode reservoirs. It was fabricated using standard photolithographic/wet chemical etching techniques followed by thermal bonding. The liquid junction is connected to a subatmospheric electrospray chamber inducing the flow inside the ESI needle and helping the ion transport via aerodynamic focusing.
Institute of Analytical Chemistry, CAS, v.v.i, Brno, Czech Republic.
Jana
J
Krenkova
Institute of Analytical Chemistry, CAS, v.v.i, Brno, Czech Republic.
Karel
K
Kleparnik
Institute of Analytical Chemistry, CAS, v.v.i, Brno, Czech Republic.
Jakub
J
Grym
Institute of Analytical Chemistry, CAS, v.v.i, Brno, Czech Republic.
Jaroslav
J
Luksch
Institute of Analytical Chemistry, CAS, v.v.i, Brno, Czech Republic.
Frantisek
F
Foret
D003911:Dextrans; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D010455:Peptides; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation*
D003911:Dextrans; D010455:Peptides
Capillary electrophoresis; Electrospray interfacing; Microfabrication