Regulated expression of virulence gene mviN provides protective immunity and colonization control of Salmonella in poultry.
Current live attenuated vaccines for control of Salmonella in poultry persist in the ceca and may persist in the environment. In this paper we report the construction and characterization of the vaccine efficacy of a Salmonella mutant strain with inducible mviN expression and rapid clearance from the host. The mutant was effective in oral immunization of the broiler chicken host against a virulent Salmonella oral challenge strain, having a mean 7×10(6)CFU/g in the ceca of unvaccinated controls compared to a mean 2×10(3)CFU/g in the ceca of vaccinated chickens at 4 weeks post-challenge (6 weeks of age). The mutant strain also demonstrated immunogenicity, reduced organ colonization, and rapid clearance in broiler chickens within 3 weeks of inoculation.
Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, AR 72704, USA.
Peter M
PM
Rubinelli
Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, AR 72704, USA.
Sang In
SI
Lee
Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, AR 72704, USA.
Stephanie M
SM
Roto
Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, AR 72704, USA.
Si Hong
SH
Park
Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, AR 72704, USA. Electronic address: sricke@uark.edu.
Steven C
SC
Ricke
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology*; D002460:Cell Line; D002470:Cell Survival; D002645:Chickens; D015964:Gene Expression Regulation, Bacterial; D007074:Immunoglobulin G / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D008297:Male; D009154:Mutation; D011200:Poultry; D012475:Salmonella / Q000235:genetics* / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control*; D013997:Time Factors; D014611:Vaccination / Q000662:veterinary; D014774:Virulence / Q000235:genetics
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G
Caco-2; HD-11; Poultry; Salmonella; Vaccine; murJ; mviN