Gold magnetic nanoparticle conjugate-based lateral flow assay for the detection of IgM class antibodies related to TORCH infections.
In this study, a lateral flow immunochromatographic assay (LFIA) system for the detection of immunoglobulin M (IgM) antibodies, related to TORCH [(T)oxoplasmosis, (O)ther agents, (R)ubella (also known as German Measles), (C)ytomegalovirus, and (H)erpes simplex virus infections], based on gold magnetic nanoparticles, was established. Following modification with poly(methacrylic acid), the gold magnetic nanoparticles conjugated with an anti‑human IgM antibody (μ‑chain specific) to construct a probe. A lateral flow assay device was constructed based on these conjugates. IgM antibodies to four types of pathogens, notably toxoplasmosis, rubella virus, cytomegalovirus and herpes simplex virus type 2, were detected using this device. Compared with commercial colloidal gold‑based LFIA strips, our method exhibited higher sensitivity. No interference with triglycerides, hemoglobin and bilirubin occurred, and no cross‑reactivity was noted among the four pathogens. The gold magnetic nanoparticle‑LFIA strips were used to assess 41 seropositive and 121 seronegative serum samples. The sensitivity was 100% (162/162) and the specificity was 100% (162/162). This method cannot only be used for the detection of TORCH IgM-specific antibodies, but it can potentially be developed for use in the diagnosis of other acute or recently identified autoimmune diseases.
College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, P.R. China.
Xingxing
X
Li
National Engineering Research Center for Miniaturized Detection Systems, Xi'an, Shaanxi 710069, P.R. China.
Qinlu
Q
Zhang
School of Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
Peng
P
Hou
School of Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
Mingwei
M
Chen
College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, P.R. China.
Wenli
W
Hui
College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, P.R. China.
Alphons
A
Vermorken
College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, P.R. China.
Zhiyi
Z
Luo
The Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 712000, P.R. China.
Hong
H
Li
The Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 712000, P.R. China.
Qin
Q
Li
College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, P.R. China.
Yali
Y
Cui
D000914:Antibodies, Viral / Q000276:immunology*; D002846:Chromatography, Affinity / Q000379:methods; D003586:Cytomegalovirus Infections / Q000276:immunology*; D006046:Gold / Q000737:chemistry*; D006561:Herpes Simplex / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D012409:Rubella / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology*
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D058185:Magnetite Nanoparticles; D006046:Gold