Increase in dicentric chromosome formation after a single CT scan in adults.
Excess risk of leukemia and brain tumors after CT scans in children has been reported. We performed dicentric chromosome assay (DCAs) before and after CT scan to assess effects of low-dose ionizing radiation on chromosomes. Peripheral blood (PB) lymphocytes were collected from 10 patients before and after a CT scan. DCA was performed by analyzing either 1,000 or 2,000 metaphases using both Giemsa staining and centromere-fluorescence in situ hybridization (Centromere-FISH). The increment of DIC formation was compared with effective radiation dose calculated using the computational dosimetry system, WAZA-ARI and dose length product (DLP) in a CT scan. Dicentric chromosome (DIC) formation increased significantly after a single CT scan, and increased DIC formation was found in all patients. A good correlation between the increment of DIC formation determined by analysis of 2,000 metaphases using Giemsa staining and those by 2,000 metaphases using Centromere-FISH was observed. However, no correlation was observed between the increment of DIC formation and the effective radiation dose. Therefore, these results suggest that chromosome cleavage may be induced by one CT scan, and we recommend 2,000 or more metaphases be analyzed in Giemsa staining or Centromere-FISH for DCAs in cases of low-dose radiation exposure.
Dept. of Radiation Life Sciences, Fukushima Medical University School of Medicine, Fukushima, Japan.
Yu
Y
Abe
Dept. of Pathologic Analysis, Hirosaki University Graduate School of Health Sciences, Hirosaki, Japan.
Tomisato
T
Miura
Dept. of Radiation Biology, Institute of Radiation Emergency Medicine, Hirosaki University, Hirosaki, Japan.
Mitsuaki A
MA
Yoshida
Dept. of Radiation Life Sciences, Fukushima Medical University School of Medicine, Fukushima, Japan.
Risa
R
Ujiie
Dept. of Radiation Life Sciences, Fukushima Medical University School of Medicine, Fukushima, Japan.
Yumiko
Y
Kurosu
Dept. of Radiation Life Sciences, Fukushima Medical University School of Medicine, Fukushima, Japan.
Nagisa
N
Kato
Dept. of Radiation Life Sciences, Fukushima Medical University School of Medicine, Fukushima, Japan.
Atsushi
A
Katafuchi
Dept. of Radiation Life Sciences, Fukushima Medical University School of Medicine, Fukushima, Japan.
Naohiro
N
Tsuyama
Dept. of Radiation Health Management, Fukushima Medical University School of Medicine, Fukushima, Japan.
Takashi
T
Ohba
Dept. of Radiation Health Management, Fukushima Medical University School of Medicine, Fukushima, Japan.
Tomoko
T
Inamasu
Dept. of Radiology, Fukushima Medical University School of Medicine, Fukushima, Japan.
Fumio
F
Shishido
Dept. of Cardiology &Hematology, Fukushima Medical University School of Medicine, Fukushima, Japan.
Hideyoshi
H
Noji
Dept. of Cardiology &Hematology, Fukushima Medical University School of Medicine, Fukushima, Japan.
Kazuei
K
Ogawa
Dept. of Pulmonary Medicine, Fukushima Medical University School of Medicine, Fukushima, Japan.
Hiroshi
H
Yokouchi
Dept. of Pulmonary Medicine, Fukushima Medical University School of Medicine, Fukushima, Japan.
Kenya
K
Kanazawa
Dept. of Pulmonary Medicine, Fukushima Medical University School of Medicine, Fukushima, Japan.
Takashi
T
Ishida
Dept. of Regenerative Surgery, Fukushima Medical University School of Medicine, Fukushima, Japan.
Satoshi
S
Muto
Dept. of Regenerative Surgery, Fukushima Medical University School of Medicine, Fukushima, Japan.
Jun
J
Ohsugi
Dept. of Regenerative Surgery, Fukushima Medical University School of Medicine, Fukushima, Japan.
Hiroyuki
H
Suzuki
Dept. of Radiation Physics and Chemistry, Fukushima Medical University School of Medicine, Fukushima, Japan.|Radiation Medical Science Center for the Fukushima Health Management Survey, Fukushima Medical University School of Medicine, Fukushima, Japan.
Tetsuo
T
Ishikawa
Dept. of Experimental Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan.|Radiation Medical Science Center for the Fukushima Health Management Survey, Fukushima Medical University School of Medicine, Fukushima, Japan.
Kenji
K
Kamiya
Dept. of Radiation Life Sciences, Fukushima Medical University School of Medicine, Fukushima, Japan.|Radiation Medical Science Center for the Fukushima Health Management Survey, Fukushima Medical University School of Medicine, Fukushima, Japan.
Akira
A
Sakai
D000368:Aged; D000369:Aged, 80 and over; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects; D008223:Lymphoma / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000532:radiotherapy; D008297:Male; D008677:Metaphase / Q000235:genetics / Q000528:radiation effects; D008875:Middle Aged; D011829:Radiation Dosage; D011839:Radiation, Ionizing; D014057:Tomography, X-Ray Computed / Q000009:adverse effects*