In vitro anti-HIV-1 activities of kaempferol and kaempferol-7-O-glucoside isolated from Securigera securidaca.
Previously, we reported that the kaempferol and kaempferol-7-O-glucoside isolated from Securigera securidaca showed potent anti-HSV activity. In the present study the anti-HIV-1 activities of kaempferol and kaempferol-7-O-glucoside are investigated at different concentrations (100, 50, 25 and 10 μg/ml) using HIV-1 p24 Antigen kit. Real-time Polymerase chain reaction (RT-PCR) assay was also used for quantification of full range of virus load observed in treated and untreated cells. According to the results of RT- PCR, tested compounds at a concentration of 100 μg/ml exerted potent inhibitory effect. Time of drug addition experiments demonstrated that these compounds exerted their inhibitory effects on the early stage of HIV infection. The results also showed potent anti-HIV-1 reverse transcriptase activity. Antiviral activity of kaempferol-7-O-glucoside was more pronounced than that of kaempferol. These findings demonstrate that kaempferol-7-O-glucoside could be considered as a new potential drug candidate for the treatment of HIV infection which requires further assessments.
Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, I.R. Iran.
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Behbahani
Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, I.R. Iran.
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Sayedipour
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
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Pourazar
Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, I.R. Iran.
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Shanehsazzadeh
Antiviral activity; Flavonol; HIV-1; Real Time PCR assay