miR-137 suppresses the invasion and procedure of EMT of human breast cancer cell line MCF-7 through targeting CtBP1.
Distant metastasis is the predominant site of gastric cancer recurrence and the most common cause of death. Recently, accumulating evidence has established that aberrant epithelial-mesenchymal transition activation plays a crucial role in the genesis, invasion, and metastasis of various cancers, including breast cancer. In this paper, we found that miR-137, which has been reported to function as a tumor suppressor in a variety of cancers, could significantly suppress the migration and invasion of MCF-7 cells, which might be correlated with its suppressive effects on the EMT procedure. Upon transfection, the epithelial marker, E-cadherin, was up-regulated, and the mesenchymal markers, N-cadherin and Vimentin, were suppressed. Moreover, we also found that carboxyl-terminal binding protein 1 (CtBP1) was a putative target gene of miR-137 in MCF-7 cells, and might be involved in the suppressive effects, which might provide novel diagnostic and therapeutic options for human breast cancer in the future.
Department of Breast and Thyroid Surgery, The Affiliated Hospital of Binzhou Medical University, Binzhou, 256603, Shandong Province, People's Republic of China.
Yong
Y
Han
Department of Respiratory Medicine, The Affiliated Hospital of Binzhou Medical University, Binzhou, 256603, Shandong Province, People's Republic of China.
Yueyang
Y
Bi
Department of General Surgery, The Jingqu Hospital of Weihai City, Weihai, 264200, Shandong Province, People's Republic of China.
Haiyang
H
Bi
Department of Gynecology and Obstetrics, The People's Hospital of South District of Qingdao, Qingdao, 266000, Shandong Province, People's Republic of China.
Caimei
C
Diao
Department of Breast and Thyroid Surgery, The Affiliated Hospital of Binzhou Medical University, Binzhou, 256603, Shandong Province, People's Republic of China.
Guoqiang
G
Zhang
Department of Breast and Thyroid Surgery, The Affiliated Hospital of Binzhou Medical University, Binzhou, 256603, Shandong Province, People's Republic of China.
Kai
K
Cheng
Department of Breast and Thyroid Surgery, The Affiliated Hospital of Binzhou Medical University, Binzhou, 256603, Shandong Province, People's Republic of China. zhenlinyangdoctor@126.com.
Zhenlin
Z
Yang
D000429:Alcohol Oxidoreductases / Q000502:physiology*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D015820:Cadherins / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics; D004268:DNA-Binding Proteins / Q000502:physiology*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics* / Q000502:physiology; D018390:Gene Targeting; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000502:physiology*; D058990:Molecular Targeted Therapy; D009361:Neoplasm Invasiveness / Q000235:genetics; D014746:Vimentin / Q000378:metabolism
D015820:Cadherins; D004268:DNA-Binding Proteins; C545567:MIRN137 microRNA, human; D035683:MicroRNAs; D014746:Vimentin; D000429:Alcohol Oxidoreductases; C096794:C-terminal binding protein
Breast cancer; CtBP1; EMT; Metastasis; miR-137