Poly[(R)-3-hydroxybutyrate] production under different salinity conditions by a novel Bacillus megaterium strain.
Bacillus megaterium uyuni S29, isolated from the Bolivian salt lake Uyuni, displays a high capability to produce poly[(R)-3-hydroxybutyrate] (PHB) in industrial culture media. In order to analyze the influence of salt on biomass formation and PHB production, cultivations at different NaCl concentrations were carried out according to the salinity conditions of the habitats of the strain's original isolation. In this preliminary report, the strain showed considerable adaptability to media of different salinity, obtaining the best results for both cellular growth and PHB production in media containing 45 g/L NaCl. The strain grew at 100 g/L NaCl and PHB production was observed even at high salt levels of 250 g/L without unwanted concurrent spore formation. Its tolerance to high salt concentrations together with auspicious PHB productivity makes this strain appealing not only for PHB production, but also for other biotechnological applications such as the treatment of salty wastewater; additional studies will be needed to further increase PHB productivity.
Departament d'Òptica i Optometria, Universitat Politècnica de Catalunya·Barcelona Tech, Sant Nebridi 22, 08222-Terrassa, Barcelona, Spain.
Alejandra
A
Rodríguez-Contreras
Institute of Chemistry, University of Graz, NAWI Graz, Heinrichstrasse 28/III, 8010 Graz, Austria; ARENA - Association for Resource Efficient and Sustainable Technologies, Inffeldgasse 21b, 8010 Graz, Austria. Electronic address: martin.koller@uni-graz.at.
Martin
M
Koller
ARENA - Association for Resource Efficient and Sustainable Technologies, Inffeldgasse 21b, 8010 Graz, Austria.
Gerhart
G
Braunegg
Departament d'Òptica i Optometria, Universitat Politècnica de Catalunya·Barcelona Tech, Sant Nebridi 22, 08222-Terrassa, Barcelona, Spain.
María Soledad
MS
Marqués-Calvo
D020155:3-Hydroxybutyric Acid / Q000096:biosynthesis*; D001410:Bacillus megaterium / Q000378:metabolism*; D018533:Biomass; D001704:Biopolymers / Q000378:metabolism*; D054712:Salinity; D017550:Spectroscopy, Fourier Transform Infrared
D001704:Biopolymers; D020155:3-Hydroxybutyric Acid