Association between promoter region genetic variants of PTH SNPs and serum 25(OH)-vitamin D level.
Parathyroid hormone (PTH) plays a crucial role in calcium metabolism and skeletal development via altering vitamin D level. Besides, hypersecretion of PTH is implicated in the etiology of osteoporosis. In this study, we analyzed association between promoter region sequence variants of PTH gene and circulating 25-hydroxy-vitamin D (25(OH)D) level. Genotypes of PTH SNPs rs1459015, rs10500783 and rs10500784 and circulating serum 25(OH)D level of healthy adults (N=386) of different nationalities living in Riyadh were determined and relation between the different PTH allelic variants and corresponding mean 25(OH)D values were obtained using Analysis of Variance (ANOVA) and Bonferroni post-hoc test for multiple comparisons. We observed a high prevalence of vitamin D deficiency (<50 nmol/l) among all nationals which ranged from 59% among Indians to 82% among Yemeni. Comparison of the means of 25(OH)D levels corresponding to different genotypes of PTH SNPs indicated that the T allele of SNP rs1459015 was associated with higher 25(OH)D level in the Sudanese (P=0.03), while the T allele of SNP rs10500783 was associated with higher 25(OH)D level in Saudis (P=0.03). Analysis of results also indicated that the Sudanese carriers of the CC genotype of SNP rs1459015 had a higher risk of suffering from vitamin D deficiency (P=0.02). In conclusion, our study indicated significant association between specific PTH gene promoter region variants and altered levels of 25(OH)D and vitamin D deficiency among specific nationals.
Biomarkers Research Program, Biochemistry Department, College of Science, King Saud University Riyadh, KSA ; Prince Mutaib Chair for Biomarkers of Osteoporosis, King Saud University Riyadh, KSA.
Nasser M
NM
Al-Daghri
Biomarkers Research Program, Biochemistry Department, College of Science, King Saud University Riyadh, KSA ; Prince Mutaib Chair for Biomarkers of Osteoporosis, King Saud University Riyadh, KSA.
Omar S
OS
Al-Attas
Biomarkers Research Program, Biochemistry Department, College of Science, King Saud University Riyadh, KSA.
Soundararajan
S
Krishnaswamy
Biomarkers Research Program, Biochemistry Department, College of Science, King Saud University Riyadh, KSA.
Sobhy M
SM
Yakout
Biomarkers Research Program, Biochemistry Department, College of Science, King Saud University Riyadh, KSA.
Abdul Khader
AK
Mohammed
School of Biological Sciences, University of Southampton Southampton SO17 1BJ, UK.
Amal M
AM
Alenad
First Department of Pediatrics, Athens University Medical School Athens, Greece.
George P
GP
Chrousos
Biomarkers Research Program, Biochemistry Department, College of Science, King Saud University Riyadh, KSA ; Prince Mutaib Chair for Biomarkers of Osteoporosis, King Saud University Riyadh, KSA.
Majed S
MS
Alokail
D000328:Adult; D003430:Cross-Sectional Studies; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D010281:Parathyroid Hormone / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D015995:Prevalence; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D012529:Saudi Arabia / Q000453:epidemiology; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood; D014808:Vitamin D Deficiency / Q000097:blood* / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics*; D055815:Young Adult
C470125:PTH protein, human; D010281:Parathyroid Hormone; D014807:Vitamin D; C104450:25-hydroxyvitamin D
25(OH)D; Parathyroid hormone; osteoporosis; single nucleotide polymorphism; vitamin D