Centerband-only-detection-of-exchange (31)P nuclear magnetic resonance and phospholipid lateral diffusion: theory, simulation and experiment.
Centerband-only-detection-of-exchange (CODEX) (31)P NMR lateral diffusion measurements were performed on dimyristoylphosphatidylcholine (DMPC) assembled into large unilamellar spherical vesicles. Optimization of sample and NMR acquisition conditions provided significant sensitivity enhancements relative to an earlier first report (Q. Saleem, A. Lai, H. Morales, and P. M. Macdonald, Chem. Phys. Lipids, 2012, 165, 721). An analytical description was developed that permitted the extraction of lateral diffusion coefficients from CODEX data, based on a Gaussian-diffusion-on-a-sphere model (A. Ghosh, J. Samuel, and S. Sinha, Europhys. Lett., 2012, 98, 30003-p1) as relevant to CODEX (31)P NMR measurements on a population of spherical unilamellar phospholipid bilayer vesicles displaying a distribution of vesicle radii.
Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6. pm.macdonald@utoronto.ca.
Angel
A
Lai
Qasim
Q
Saleem
Peter M
PM
Macdonald
D004058:Diffusion; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D000067493:Dynamic Light Scattering; D009682:Magnetic Resonance Spectroscopy; D010743:Phospholipids / Q000737:chemistry*; D010758:Phosphorus / Q000737:chemistry; D053835:Unilamellar Liposomes / Q000737:chemistry
D010743:Phospholipids; D053835:Unilamellar Liposomes; D010758:Phosphorus; D004134:Dimyristoylphosphatidylcholine