Competitive and noncompetitive phage immunoassays for the determination of benzothiostrobin.
Twenty-three phage-displayed peptides that specifically bind to an anti-benzothiostrobin monoclonal antibody (mAb) in the absence or presence of benzothiostrobin were isolated from a cyclic 8-residue peptide phage library. Competitive and noncompetitive phage enzyme linked immunosorbent assays (ELISAs) for benzothiostrobin were developed by using a clone C3-3 specific to the benzothiostrobin-free mAb and a clone N6-18 specific to the benzothiostrobin immunocomplex, respectively. Under the optimal conditions, the half maximal inhibition concentration (IC50) of the competitive phage ELISA and the concentration of analyte producing 50% saturation of the signal (SC50) of the noncompetitive phage ELISA for benzothiostrobin were 0.94 and 2.27 ng mL(-1), respectively. The noncompetitive phage ELISA showed higher selectivity compared to the competitive. Recoveries of the competitive and the noncompetitive phage ELISAs for benzothiostrobin in cucumber, tomato, pear and rice samples were 67.6-119.6% and 70.4-125.0%, respectively. The amounts of benzothiostrobin in the containing incurred residues samples detected by the two types of phage ELISAs were significantly correlated with that detected by high-performance liquid chromatography (HPLC).
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
Xiude
X
Hua
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
Liangliang
L
Zhou
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
Lu
L
Feng
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
Yuan
Y
Ding
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
Haiyan
H
Shi
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
Limin
L
Wang
Department of Entomology and UCD Cancer Center, University of California, Davis, CA 95616, United States.
Shirley J
SJ
Gee
Department of Entomology and UCD Cancer Center, University of California, Davis, CA 95616, United States.
Bruce D
BD
Hammock
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China. Electronic address: wangmha@njau.edu.cn.
Minghua
M
Wang
D000179:Acrylates / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D052160:Benzothiazoles / Q000032:analysis* / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D018553:Cucumis sativus / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D018551:Solanum lycopersicum / Q000737:chemistry; D012275:Oryza / Q000737:chemistry; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry; D031989:Pyrus / Q000737:chemistry
D000179:Acrylates; D000911:Antibodies, Monoclonal; D052160:Benzothiazoles; D019151:Peptide Library; D010455:Peptides; C000598462:benzothiostrobin
Benzothiostrobin; Enzyme-linked immunosorbent assay; Noncompetitive immunoassay; Peptidomimetics; Phage anti-immunocomplex assay; Phage-displayed peptide