Keratan sulfate exacerbates experimental autoimmune encephalomyelitis.
Proteoglycans (PGs) are the components of extracellular matrices in the central nervous system (CNS). Keratan sulfate (KS) is a glycosaminoglycan that is included in the KSPG that acts as an inhibitory factor in nerve regeneration after CNS injury. To investigate the role of KS in immune diseases, we induced experimental autoimmune encephalomyelitis (EAE) in mice that were deficient in the N-acetylglucosamine (GlcNAc)-6-O-sulfotransferase 1 (GlcNAc6ST1) gene (KS-KO). KS-KO mice developed less severe EAE and showed repressed recall response in the induction phase. Furthermore, GlcNAc6ST1 might have roles in the passage of the pathogenic lymphocytes through the blood-brain barrier via adhesion molecules. Thus, modulation of KS may become a treatment for neuroimmunological diseases.
Department of Neurology, Kinki University School of Medicine, Osaka-Sayama, Japan.
Rino
R
Ueno
Department of Neurology, Kinki University School of Medicine, Osaka-Sayama, Japan.
Katsuichi
K
Miyamoto
Department of Neurology, Kinki University School of Medicine, Osaka-Sayama, Japan.
Noriko
N
Tanaka
Division of Neurology, Department of Internal Medicine 3, National Defense Medical College, Tokorozawa, Japan.
Kota
K
Moriguchi
Department of Biochemistry, Nagoya University School of Medicine, Nagoya, Japan.
Kenji
K
Kadomatsu
Department of Neurology, Kinki University School of Medicine, Osaka-Sayama, Japan.
Susumu
S
Kusunoki
D019264:Adoptive Transfer / Q000009:adverse effects; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced* / Q000235:genetics* / Q000276:immunology; D005620:Freund's Adjuvant / Q000633:toxicity; D007632:Keratan Sulfate / Q000633:toxicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000633:toxicity; D010446:Peptide Fragments / Q000633:toxicity; D015238:Sulfotransferases / Q000172:deficiency / Q000235:genetics; D013601:T-Lymphocytes / Q000187:drug effects / Q000473:pathology; D000097811:Carbohydrate Sulfotransferases
D016207:Cytokines; D005620:Freund's Adjuvant; D007632:Keratan Sulfate; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D015238:Sulfotransferases; D000097811:Carbohydrate Sulfotransferases; C445081:myelin oligodendrocyte glycoprotein (35-55)
central nervous system; experimental autoimmune encephalomyelitis; glycosaminoglycan; keratan sulfate; multiple sclerosis