Modeling tailed bacteriophage adsorption: Insight into mechanisms.
The process of a bacteriophage attaching to its host cell is a combination of physical diffusion, biochemical surface interactions, and reaction-induced conformational changes in receptor proteins. Local variations in the physico-chemical properties of the medium, the phage׳s mode of action, and the physiology of the host cell also all influence adsorption kinetics. These characteristics can affect a specific phage׳s binding capabilities and the susceptibility of the host cell to phage attack. Despite the complexity of this process, describing adsorption kinetics of a population of bacteriophages binding to a culture of cells has been accomplished with relatively simple equations governed by the laws of mass-action. Many permutations and modifications to the basic set of reactions have been suggested through the years. While no single solution emerges as a universal answer, this review provides the fundamentals of current phage adsorption modeling and will guide researchers in the selection of valid, appropriate models.
Department of Chemical and Materials Engineering, University of Alberta, 9107-116th Street, Edmonton, Alberta, Canada T6G 2V4.
Zachary J
ZJ
Storms
Department of Chemical and Materials Engineering, University of Alberta, 9107-116th Street, Edmonton, Alberta, Canada T6G 2V4. Electronic address: dominic.sauvageau@ualberta.ca.
Dominic
D
Sauvageau
D000465:Algorithms; D001419:Bacteria / Q000821:virology*; D001435:Bacteriophages / Q000502:physiology*; D007700:Kinetics; D008954:Models, Biological; D053585:Virus Attachment
Adsorption efficiency; Adsorption kinetics; Phage amplification; Phage therapy; Sequential model