The protective effect of G-CSF on experimental ischemia/reperfusion injury in rat ovary.
OBJECTIVE
This experimental study was designed to evaluate the effect of granulocyte colony-stimulating factor (G-CSF) in ovarian ischemia and ischemia/reperfusion (I/R) injury.


METHODS
Forty-eight female adult Sprague-Dawley albino rats were divided into six groups as Group 1: sham, Group 2: torsion, Group 3: detorsion, Group 4: sham + G-CSF, Group 5: torsion + G-CSF, and Group 6: detorsion + G-CSF. Except for Groups 1 and 4, all groups underwent a dnexal torsion bilaterally for 3 h. Adnexal detorsion was applied to Groups 3 and 6 for 3 h after a 3-h torsion period. The intraperitoneal injection of G-CSF (100 IU/kg) was administered 30 min previously in Group 4, 5 and 6. At the end of the study process the animals were euthanized and their ovaries were removed for histopathological and biochemical analysis. Total oxidant status (TOS), total antioxidative status and oxidative stress index (OSI) concentrations were determined and compared. Histopathological examination of ovaries was performed for the presence of interstitial edema, congestion, hemorrhage and loss of cohesion to determine tissue damage.


RESULTS
In Group 3, 4, 5 and 6, TOS, OSI and total histopathological scores of ovarian tissue were higher than in the sham group (p < 0.05). G-CSF administration decreased mean TOS and OSI levels significantly when compared with the controls (p < 0.001, p < 0.001, respectively). There was a strong correlation between the total histopathological scores of I/R injury and OSI (r = 0.862, p < 0.001). The total histopathological scores for the rats conservatively treated with G-CSF were lower than those of the control groups.


CONCLUSIONS
G-CSF is effective for the prevention of ischemia and ischemia/reperfusion-induced damage in rat ovary.
Department of Obstetrics and Gynecology, Sakarya University, Medical Faculty, 54100, Sakarya, Turkey. msbostanci@sakarya.edu.tr.
Mehmet Sühha
MS
Bostancı
Department of Obstetrics and Gynecology, Kahramanmaras Sutcu Imam University Medical Faculty, Kahramanmaras, Turkey.
Murat
M
Bakacak
Department of Biochemistry, Kahramanmaras Sutcu Imam University Medical Faculty, Kahramanmaras, Turkey.
Fatma
F
İnanc
Department of Pathology, Kahramanmaras Sutcu Imam University Medical Faculty, Kahramanmaras, Turkey.
Aslı
A
Yaylalı
Department of Obstetrics and Gynecology, Kahramanmaras Sutcu Imam University Medical Faculty, Kahramanmaras, Turkey.
Salih
S
Serin
Department of Obstetrics and Gynecology, Yeditepe University Medical Faculty, Istanbul, Turkey.
Rukset
R
Attar
Department of Obstetrics and Gynecology, Yeditepe University Medical Faculty, Istanbul, Turkey.
Özge Kızılkale
ÖK
Yildirim
Department of Obstetrics and Gynecology, Yeditepe University Medical Faculty, Istanbul, Turkey.
Gazi
G
Yildirim
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D007511:Ischemia; D010049:Ovarian Diseases / Q000188:drug therapy*; D010053:Ovary / Q000098:blood supply* / Q000187:drug effects* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*
D000975:Antioxidants; D016179:Granulocyte Colony-Stimulating Factor
Granulocyte colony-stimulating factor; Ischemia/reperfusion injury; Ovarian torsion; Oxidative stress