The modulation of biodistribution of stem cells by anchoring lipid-conjugated heparin on the cell surface.
Heparin is a bioactive glycosaminoglycan that can interact with various extracellular matrix (ECM) proteins and growth factors. Lipid-conjugated heparin was synthesized, and was used to coat adipose-derived stem cells (ADSCs) by physical insertion on the cell membrane. Coating of lipid-conjugated heparin with two lipid moieties on ADSCs was stable for 24h in vitro. Biodistribution of heparin-coated ADSCs upon intravenous injection in mice was analyzed by In-Vivo Imaging System (IVIS), and showed enhanced accumulation in the liver and spleen while reduced entrapment in the lung. Thus, the coating of ADSCs with lipid-conjugated heparin could significantly modulate the biodistribution of cells.
School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea.
Jong Chul
JC
Kim
School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea. Electronic address: gytae@gist.ac.kr.
Giyoong
G
Tae
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D008099:Liver / Q000378:metabolism; D008297:Male; D008807:Mice, Inbred BALB C; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D013154:Spleen / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology; D014018:Tissue Distribution
D010714:Phosphatidylethanolamines; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; D006493:Heparin
Biodistribution; Cell surface engineering; Lipid-conjugated heparin; Stem cell