Oral Administration of Recombinant Lactococcus lactis Expressing the Cellulase Gene Increases Digestibility of Fiber in Geese.
Enhancing cellulose digestibility in animals is important for improving the utilization of forage, which can decrease the amount of food used in animal production. The aim of the present study was to achieve recombinant expression of the cellulase gene in Lactococcus lactis and evaluate the effects of oral administration of the recombinant L. lactis on fiber digestibility in geese. Cellulase (Cell) and green fluorescent protein (GFP) genes were cloned into a L. lactis expression vector (pNZ8149) to construct the recombinant expression plasmid (pNZ8149-GFP-Cell). Then, the recombinant expression plasmid was transformed into L. lactis (NZ3900) competent cells by electroporation to obtain recombinant L. lactis (pNZ8149-GFP-Cell/NZ3900) in which protein expression was induced by Nisin. Expression of GFP and Cell by the recombinant L. lactis was confirmed using SDS-PAGE, fluorescence detection, and Congo red assays. A feeding experiment showed that oral administration of pNZ8149-GFP-Cell/NZ3900 significantly increased the digestibility of dietary fiber in geese fed either a maize stalk diet or a rice chaff diet. Therefore, oral administration of recombinant L. lactis cells expressing the cellulase gene increases fiber digestibility in geese, offering a way to increase the utilization of dietary fiber in geese.
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Haizhu
H
Zhou
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Yunhang
Y
Gao
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Guang
G
Gao
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China. louyujiejlau@126.com.
Yujie
Y
Lou
D000284:Administration, Oral; D000818:Animals; D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D004032:Diet / Q000379:methods; D004043:Dietary Fiber / Q000378:metabolism*; D005777:Geese; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D030781:Organisms, Genetically Modified; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D004043:Dietary Fiber; D011994:Recombinant Proteins; D002480:Cellulase