Purification, characterization and immunomodulatory activity of a polysaccharide from Celosia cristata.
A polysaccharide CP1-1 was isolated and purified from Celosia cristata. Its average molecular weight was 2.3kDa and it was composed of glucose and galactose in a ratio of 1.00:2.03, and traces of mannose. Chemical characterization of CP1-1 was elucidated by methylation analysis. CP1-1 was a branched glucogalactan which was mainly composed of 1,6-linked Galp and 1,6-linked Glcp with a ratio of 5.6:3.8. The branches were at the O-3 of the main chain and might be composed of single terminal (1→)-linked glucopyranose and galactopyranose. CP1-1 also significantly promoted the proliferation and neutral red phagocytosis of RAW 264.7 macrophage cells in vitro. In addition, CP1-1 promoted cell proliferation by enhancing the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin (IL)-6 and IL-1β. These results suggested that the polysaccharide from C. cristata could be used as a potential immunostimulator.
State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 200040, China; Fengxian Hospital affiliated to Southern Medical University, Shanghai 201499, China.
Zhenliang
Z
Sun
Fengxian Hospital affiliated to Southern Medical University, Shanghai 201499, China.
You
Y
Peng
Fengxian Hospital affiliated to Southern Medical University, Shanghai 201499, China.
Wei-Wei
WW
Zhao
Fengxian Hospital affiliated to Southern Medical University, Shanghai 201499, China. Electronic address: linlinxiao2015@163.com.
Lin-Lin
LL
Xiao
State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 200040, China. Electronic address: peimingy@163.com.
Pei-Ming
PM
Yang
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D034762:Celosia / Q000737:chemistry*; D016207:Cytokines / Q000096:biosynthesis; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008745:Methylation; D051379:Mice; D009005:Monosaccharides / Q000032:analysis; D009569:Nitric Oxide / Q000096:biosynthesis; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000067996:RAW 264.7 Cells; D012639:Seeds / Q000737:chemistry
D016207:Cytokines; D007155:Immunologic Factors; D009005:Monosaccharides; D011134:Polysaccharides; D009569:Nitric Oxide
Celosia cristata; Immunomodulatory activity; Macrophages; Polysaccharide