An electrochemical molecular recognition-based aptasensor for multiple protein detection.
This article reports a simple electrochemical approach for the detection of multiple proteins (thrombin and lysozyme) using Dabcyl-labeled aptamer modified metal nanoparticles (DLAPs). DLAPs were immobilized on β-cyclodextrins (β-CDs) modified electrode by means of host-guest self-assembly. During the time of detection, the aptamers' structure will change due to the specific binding with corresponding proteins that forced DLAPs far away from the electrode that had been modified by β-CDs. Thus, the capture of target proteins onto DLAPs was translated via the electrochemical current signal offered by metal nanoparticles. Linearity of the aptasensor for quantitative measurements was demonstrated. Determinations of proteins in human real serum samples were also performed to demonstrate detection in real clinical samples.
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, People's Republic of China.
Lin
L
Cheng
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, People's Republic of China.
Jie
J
Zhang
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, People's Republic of China.
Yan
Y
Lin
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, People's Republic of China.
Qiong
Q
Wang
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, People's Republic of China.
XiuXiu
X
Zhang
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, People's Republic of China.
YanHua
Y
Ding
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, People's Republic of China. Electronic address: cuihanfeng@126.com.
Hanfeng
H
Cui
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, People's Republic of China. Electronic address: fanhao11@aliyun.com.
Hao
H
Fan
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D009113:Muramidase / Q000032:analysis*; D013917:Thrombin / Q000032:analysis*; D047392:beta-Cyclodextrins / Q000737:chemistry
D052157:Aptamers, Nucleotide; D047392:beta-Cyclodextrins; D009113:Muramidase; D013917:Thrombin
Aptasensor; Electrochemical; Molecular recognition; Multiple protein detection