Spin column extraction as a new sample preparation method in bioanalysis.
Sample preparation is important in obtaining accurate data for qualification and quantification in bioanalysis. We have recently focused on monolithic silica for high-throughput analysis. These extraction processes - using monolithic silica packed in spin column - such as sample loading, washing and elution, are executed by centrifugation. There are several possibilities such as on-column derivatization for the determination of amines or carboxylic acids in the sample. The spin column extraction reduces the sample preparation time required for determination of drugs and other chemicals in biological materials and increases productivity in bioanalysis. We expect spin column extraction to become the mainstream method of sample processing in the future.
Department of Forensic Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, 1-2-3 Kasumi, Hiroshima 734-8553, Japan.
Akira
A
Namera
Department of Emergency & Critical Care Medicine, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1143, Japan.
Takashi
T
Saito
D000327:Adsorption; D053000:Analytic Sample Preparation Methods / Q000379:methods*; D005591:Chemical Fractionation / Q000379:methods*; D019963:Clinical Chemistry Tests / Q000379:methods*; D006801:Humans