Coherent correlation networks among protein biomarkers of beef tenderness: What they reveal.
The development of proteomic biomarkers for meat tenderness remains an important challenge. The present study used Longissimus thoracis (LT) and Semitendinosus (ST) muscles of young bulls of three continental breeds (Aberdeen Angus, Blond d'Aquitaine and Limousin) to i) identify cellular pathways robustly related with meat tenderness, using potential protein biomarkers and ii) describe biochemical mechanisms underlying muscle to meat conversion. Correlation networks reveal robust correlations, i.e. present for at least two breeds, between potential meat tenderness biomarkers. For the two muscles of the three breeds, DJ-1 and Peroxiredoxin 6 were consistently correlated with Hsp20 and μ-calpain, respectively. For the three breeds, μ-calpain was related to Hsp70-8 in the LT muscle. Various correlations were muscle specific. For the three breeds, DJ-1 was correlated with Hsp27 for the ST, and with ENO3 and LDH-B for the LT muscle. Overall, in the LT, more correlations were found between proteins related to the glycolytic pathway and in the ST, with the small Hsps (Hsp20, 27 and αB-crystallin). Hsp70-Grp75 appeared involved in several relevant biological pathways. At the scientific level, results give insights in biological functions involved in meat tenderness. Further studies are needed to confirm the possible use of these biomarkers in the meat industry to improve assurance of good meat qualities.
INRA, UMR 1213 Herbivores, F-63122 Saint-Genès-Champanelle, France; Clermont Université, VetAgro Sup, UMR 1213 Herbivores, BP 10448, F-63000 Clermont-Ferrand, France; Equipe Maquav, INATAA, Université Frères Mentouri Constantine, Route de Ain El-Bey, 25000, Constantine, Algeria.
Mohammed
M
Gagaoua
INRA, UMR 1213 Herbivores, F-63122 Saint-Genès-Champanelle, France; Clermont Université, VetAgro Sup, UMR 1213 Herbivores, BP 10448, F-63000 Clermont-Ferrand, France.
E M Claudia
EM
Terlouw
Equipe Maquav, INATAA, Université Frères Mentouri Constantine, Route de Ain El-Bey, 25000, Constantine, Algeria.
Abdelghani
A
Boudjellal
INRA, UMR 1213 Herbivores, F-63122 Saint-Genès-Champanelle, France; Clermont Université, VetAgro Sup, UMR 1213 Herbivores, BP 10448, F-63000 Clermont-Ferrand, France. Electronic address: brigitte.picard@clermont.inra.fr.
Brigitte
B
Picard
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D005504:Food Analysis / Q000379:methods*; D006244:Hardness / Q000502:physiology; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000502:physiology*; D000069466:Red Meat / Q000032:analysis / Q000145:classification*; D015398:Signal Transduction / Q000502:physiology*; D013223:Statistics as Topic
D015415:Biomarkers; D006360:Heat-Shock Proteins; D009124:Muscle Proteins
Beef; Biomarkers; Breed; Correlation networks; Meat tenderness; Muscle