Expression of human amyloid precursor protein in Drosophila melanogaster nerve cells causes a decrease in presynaptic gene mRNA levels.
Amyloid precursor protein (APP) is a key player in Alzheimer's disease. The proteolytic cleavage of APP results in various short peptide fragments including the toxic amyloid-beta peptide, which is a main component of senile plaques. However, the functions of APP and its processed fragments are not yet well understood. Here, using real-time polymerase chain reaction, we demonstrate that exogenous expression of APP, its mutant form APP-Swedish, or two truncated forms in Drosophila melanogaster causes a significant (P ≤ 0.05) drop in the mRNA levels of the presynaptic proteins synaptotagmin-1 and neuronal synaptobrevin. The results obtained from this study suggest a potential role of APP or its fragments in the regulation of synaptic gene transcription.
Molecular and Radiation Biophysics Division, National Research Centre "Kurchatov Institute" B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russia rodin.dmitry@icloud.com.
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Rodin
Molecular and Radiation Biophysics Division, National Research Centre "Kurchatov Institute" B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russia.
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Schwarzman
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Sarantseva
D016564:Amyloid beta-Protein Precursor / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D009474:Neurons / Q000378:metabolism*; D053763:Presenilins / Q000235:genetics / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism*; D050683:R-SNARE Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D050863:Synaptotagmin I / Q000235:genetics / Q000378:metabolism
C545487:APP protein, human; D016564:Amyloid beta-Protein Precursor; D029721:Drosophila Proteins; D053763:Presenilins; C106427:Psn protein, Drosophila; D050683:R-SNARE Proteins; D012333:RNA, Messenger; D050863:Synaptotagmin I; C084068:nsyb protein, Drosophila