A balanced microbiota efficiently produces methane in a novel high-rate horizontal anaerobic reactor for the treatment of swine wastewater.
A novel combination of structurally simple, high-rate horizontal anaerobic reactors installed in series was used to treat swine wastewater. The reactors maintained stable pH, alkalinity, and volatile acid levels. Removed chemical oxygen demand (COD) represented 68% of the total, and the average specific methane production was 0.30L CH4 (g removed CODtot)(-1). In addition, next-generation sequencing and quantitative real-time PCR analyses were used to explore the methane-producing Archaea and microbial diversity. At least 94% of the sludge diversity belong to the Bacteria and Archaea, indicating a good balance of microorganisms. Among the Bacteria the Proteobacteria, Bacteroidetes and Firmicutes were the most prevalent phyla. Interestingly, up to 12% of the sludge diversity belongs to methane-producing orders, such as Methanosarcinales, Methanobacteriales and Methanomicrobiales. In summary, this system can efficiently produce methane and this is the first time that horizontal anaerobic reactors have been evaluated for the treatment of swine wastewater.
Faculty of Agricultural and Veterinary Sciences, Univ Estadual Paulista, Campus of Jaboticabal, Department of Rural Engineering, Laboratory of Environmental Sanitation, 14884-900, Jaboticabal, SP, Brazil; Faculty of Technology Jaboticabal, 14883-130, Jaboticabal, SP, Brazil. Electronic address: rosemariaduda@hotmail.com.
Rose Maria
RM
Duda
Faculty of Agricultural and Veterinary Sciences, Univ Estadual Paulista, Campus of Jaboticabal, Department of Rural Engineering, Laboratory of Environmental Sanitation, 14884-900, Jaboticabal, SP, Brazil.
Juliana
J
da Silva Vantini
Faculty of Agricultural and Veterinary Sciences, Univ Estadual Paulista, Campus of Jaboticabal, Department of Rural Engineering, Laboratory of Environmental Sanitation, 14884-900, Jaboticabal, SP, Brazil.
Larissa Scattolin
LS
Martins
Faculty of Agricultural and Veterinary Sciences, Univ Estadual Paulista, Campus of Jaboticabal, Department of Technology, Laboratory of Biochemistry and Molecular Biology, 14884-900, Jaboticabal, SP, Brazil.
Alessandro
A
de Mello Varani
Faculty of Agricultural and Veterinary Sciences, Campus of Jaboticabal, Department of Applied Biology, Bacterial Genetics Laboratory, 14884-900, Jaboticabal, SP, Brazil.
Manoel Victor Franco
MV
Lemos
Faculty of Agricultural and Veterinary Sciences, Univ Estadual Paulista, Campus of Jaboticabal, Department of Technology, Laboratory of Biochemistry and Molecular Biology, 14884-900, Jaboticabal, SP, Brazil.
Maria Inês Tiraboschi
MI
Ferro
Faculty of Agricultural and Veterinary Sciences, Univ Estadual Paulista, Campus of Jaboticabal, Department of Rural Engineering, Laboratory of Environmental Sanitation, 14884-900, Jaboticabal, SP, Brazil.
Roberto Alves
RA
de Oliveira
D000693:Anaerobiosis; D000818:Animals; D001105:Archaea / Q000145:classification* / Q000378:metabolism; D001419:Bacteria / Q000145:classification* / Q000378:metabolism; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D008697:Methane / Q000096:biosynthesis; D017018:Methanomicrobiales / Q000235:genetics; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D012722:Sewage / Q000382:microbiology; D013552:Swine; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology*; D014874:Water Pollutants, Chemical
D008697:Methane; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical
Biogas; Methanogenic archaea; Next generation sequencing; Quantitative real-time PCR; Renewable energy