Simultaneous Enhancement of Intracellular Optical Imaging and Photothermal Therapeutic Response by Octaarginine-Modified Fluorescent Dye Doped Pd@Ag@SiO2(RITC) Multifunctional Nanoparticles.
In the work, a novel multifunctional silica-based nanoplatform (Pd@Ag@SiO2(RITC)-R8) for bioimaging and photothermal therapy (PTT) of cancer cells has been developed. The Pd@Ag nanosheets encapsulated inside silica can act as effective near-infrared (NIR) absorbers for cancer photothermal therapy. Fluorescent dye, rhodamine B isothiocyanate (RITC), was covalently doped into the silica network to provide the capacity for optical imaging. After amine modification, the Pd@Ag@SiO2(RITC)-NH2 can be further conjugated with octaarginine (R8, a cell penetrating peptide) for enhancing the uptake of nanoparticles by cells. Confocal fluorescent images and flow cytometry analysis revealed that R8-conjugated nanoparticles (Pd@Ag@SiO2(RITC)-R8) were taken up by cells more efficiently. Correspondingly, the optical imaging and photothermal therapeutic efficiency of Pd@Ag@SiO2(RITC)-R8 upon cancer cells were also raised due to their higher cellular uptake when compared with that of Pd@Ag@SiO2(RITC)-NH2. Our results indicate that these multifunctional Pd@Ag@SiO2(RITC)-R8 may have great potential for applications in imaging-guided cancer photothermal therapy.
Saige
S
Shi
Xianglong
X
Zhu
Yizhuan
Y
Huang
Mengying
M
Jiang
Zengxia
Z
Zhao
Xiaolan
X
Chen
D002470:Cell Survival / Q000187:drug effects; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000627:therapeutic use; D006367:HeLa Cells; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000627:therapeutic use*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology*; D009842:Oligopeptides / Q000737:chemistry / Q000493:pharmacokinetics; D010316:Particle Size; D010778:Photochemotherapy / Q000379:methods*; D011838:Radiation-Sensitizing Agents / Q000737:chemistry / Q000627:therapeutic use; D012235:Rhodamines / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D012834:Silver / Q000627:therapeutic use; D016896:Treatment Outcome
D005456:Fluorescent Dyes; D009842:Oligopeptides; D011838:Radiation-Sensitizing Agents; D012235:Rhodamines; C448619:octaarginine; D012834:Silver; D006046:Gold; D012822:Silicon Dioxide; C027755:rhodamine isothiocyanate