The polyamine oxidase from lycophyte Selaginella lepidophylla (SelPAO5), unlike that of angiosperms, back-converts thermospermine to norspermidine.
In the phylogeny of plant polyamine oxidases (PAOs), clade III members from angiosperms, such as Arabidopsis thaliana PAO5 and Oryza sativa PAO1, prefer spermine and thermospermine as substrates and back-convert both of these substrates to spermidine in vitro. A clade III representative of lycophytes, SelPAO5 from Selaginella lepidophylla, also prefers spermine and thermospermine but instead back-converts these substrates to spermidine and norspermidine, respectively. This finding indicates that the clade III PAOs of lycophytes and angiosperms oxidize thermospermine at different carbon positions. We discuss the physiological significance of this difference.
Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan.
G H M
GH
Sagor
Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan.
Masataka
M
Inoue
Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan.
Dong Wook
DW
Kim
Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan; Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Aramaki aza Aoba 6-3, Aoba, Sendai 980-8578, Japan.
Seiji
S
Kojima
Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama 350-0295, Japan.
Masaru
M
Niitsu
Biodiversity and Climate Research Center, Laboratory Center, Georg-Voigt-Str. 14-16, D-60325 Frankfurt am Main, Germany.
Thomas
T
Berberich
Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan. Electronic address: kusano@ige.tohoku.ac.jp.
Tomonobu
T
Kusano
D002851:Chromatography, High Pressure Liquid; D003681:Dehydration; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015394:Molecular Structure; D010084:Oxidation-Reduction; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D032503:Selaginellaceae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013053:Spectrophotometry; D013095:Spermidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D013096:Spermine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014867:Water / Q000378:metabolism / Q000494:pharmacology; D000097667:Polyamine Oxidase
D000587:Oxidoreductases Acting on CH-NH Group Donors; D010940:Plant Proteins; D013095:Spermidine; D013096:Spermine; D014867:Water; D000097667:Polyamine Oxidase; C006760:norspermidine; C021097:thermospermine
Norspermidine; Polyamine oxidase; Selaginella lepidophylla; Spermidine; Spermine; Thermospermine