A homologue gene of β-catenin participates in the development of shrimps and immune response to bacteria and viruses.
β-Catenin is a multifunctional protein that is involved in many physiological processes, including development, cell proliferation, cell migration, and apoptosis. However, the function of β-Catenin in crustacean is unknown. In this study, the first shrimp homologous gene of β-catenin in Marsupenaeus japonicus (i.e., Mjβ-catenin) was identified and characterized. The full-length of the complementary DNA of Mjβ-catenin is 3130 bp, including a 2463 bp open reading frame that encodes 821 amino acid. Multiple alignment of β-Catenin proteins suggested that the Armadillo/β-Catenin-like repeat domains were conserved. Phylogenetic analysis showed that β-Catenin from shrimp was clustered into one group with invertebrate β-Catenin. The transcription of β-catenin in various development stages of shrimp was detected and persistently increased as the shrimp matured. In adult shrimp, β-catenin was widely distributed in detected tissues and has the relatively high expression level in gills, hemocytes, testes, and ovaries. The transcripts of β-catenin in tissues of adult shrimp were significantly up-regulated at various time points after infecting with Staphylococcus aureus, Vibrio anguillarum, and white-spot syndrome virus. Furthermore, knockdown of β-catenin resulted in impaired bacterial clearance ability and increased virus copy in shrimp in vivo. Therefore, β-Catenin in shrimp participates in the development and immune response of shrimps.
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology; School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China.
Ya-Kai
YK
Xie
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology; School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China.
Ding
D
Ding
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology; School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China.
Hui-Min
HM
Wang
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology; School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China. Electronic address: cjkang@sdu.edu.cn.
Cui-Jie
CJ
Kang
D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D007113:Immunity, Innate; D009928:Organ Specificity; D033561:Penaeidae / Q000235:genetics* / Q000254:growth & development / Q000276:immunology / Q000378:metabolism; D010802:Phylogeny; D013211:Staphylococcus aureus / Q000502:physiology; D014733:Vibrio / Q000502:physiology; D046848:White spot syndrome virus 1 / Q000502:physiology; D051176:beta Catenin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D060829:Arthropod Proteins; D051176:beta Catenin
Development; Immune response; RNA interference; Shrimp; β-catenin