6,6'-Bieckol protects insulinoma cells against high glucose-induced glucotoxicity by reducing oxidative stress and apoptosis.
Pancreatic β cells are highly sensitive to oxidative stress, which might play an important role in β cell death in diabetes. The protective effect of 6,6'-bieckol, a phlorotannin polyphenol compound purified from Ecklonia cava, against high glucose-induced glucotoxicity was investigated in rat insulinoma cells. High glucose (30 mM) treatment induced the death of rat insulinoma cells, but treatment with 10 or 50 μg/mL 6,6'-bieckol significantly inhibited the high glucose-induced glucotoxicity. Furthermore, treatment with 6,6'-bieckol dose-dependently reduced the level of thiobarbituric acid reactive substances, generation of intracellular reactive oxygen species, and the level of nitric oxide, all of which were increased by high glucose concentration. In addition, 6,6'-bieckol protected rat insulinoma cells from apoptosis under high-glucose conditions. These effects were associated with increased expression of the anti-apoptotic protein Bcl-2 and reduced expression of the pro-apoptotic protein Bax. These findings indicate that 6,6'-bieckol could be used as a potential nutraceutical agent offering protection against the glucotoxicity caused by hyperglycemia-induced oxidative stress associated with diabetes.
Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan, 617-736, Republic of Korea.
Mi-Hwa
MH
Park
Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology, Jeju, 63349, Republic of Korea.
Soo-Jin
SJ
Heo
Jeju center, Korea Basic Science Institute (KBSI), Jeju, 690-140, Republic of Korea.
Kil-Nam
KN
Kim
Department of Marine Bio-Food Science, Chonnam National University, Yeosu, 550-749, Republic of Korea.
Ginnae
G
Ahn
Department of Biotechnology, Konkuk University, Chungju, 380-701, Republic of Korea.
Pyo-Jam
PJ
Park
Division of Food Bioscience, and Korea Nokyong Research Center, Konkuk University, Chungju, 380-701, Republic of Korea.
Sang-Ho
SH
Moon
Division of Food Bioscience, and Korea Nokyong Research Center, Konkuk University, Chungju, 380-701, Republic of Korea.
Byong-Tae
BT
Jeon
Division of Food Bioscience, and Korea Nokyong Research Center, Konkuk University, Chungju, 380-701, Republic of Korea. Electronic address: leesh80@kku.ac.kr.
Seung-Hong
SH
Lee
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004147:Dioxins / Q000494:pharmacology*; D005947:Glucose / Q000009:adverse effects; D007340:Insulinoma / Q000473:pathology*; D015227:Lipid Peroxidation; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010190:Pancreatic Neoplasms / Q000473:pathology*; D000459:Phaeophyceae / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
C494828:Bax protein, rat; D004147:Dioxins; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D051028:bcl-2-Associated X Protein; D009569:Nitric Oxide; C062360:6,6'-bieckol; D005947:Glucose
6,6′-bieckol; Apoptosis; High glucose; Oxidative stress; Rat insulinoma cells