[Analysis of Chlorophyll Fluorescence Transient by Spectral Multi-exponential Approximation].
The method for analysis of chlorophyll fluorescence transient using approximation of measured signal by multi-exponential series is described. Visualization of partial sums of this series allows us to find amplitudes and characteristic times of individual phases of fluorescence induction curve. This method gives more rigid criteria of phase identification instead of semi-empirical approach currently used. Applied to Chlamidomonas reinhardtii sulfur deprivation case, it shows efficiency in finding visually undistinguishable phases of fluorescence transient for early detection of stress.
T Yu
TY
Plyusnina
S S
SS
Klruschev
G Yu
GY
Riznichenko
A B
AB
Rubin
D016825:Chlamydomonas reinhardtii / Q000187:drug effects / Q000502:physiology*; D002734:Chlorophyll / Q000032:analysis* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D005453:Fluorescence; D007700:Kinetics; D008027:Light; D010788:Photosynthesis / Q000502:physiology; D045332:Photosystem II Protein Complex / Q000502:physiology*; D013050:Spectrometry, Fluorescence / Q000706:statistics & numerical data*; D013312:Stress, Physiological; D013455:Sulfur / Q000172:deficiency; D013997:Time Factors
D003470:Culture Media; D045332:Photosystem II Protein Complex; D002734:Chlorophyll; D013455:Sulfur