Combined effects of flow-induced shear stress and electromagnetic field on neural differentiation of mesenchymal stem cells.
Differentiation of bone marrow-derived mesenchymal stem cells (MSCs) into neural phenotype has been induced by either flow-induced shear stress (FSS) or electromagnetic fields (EMF). However, procedures are still expensive and time consuming. In the present work, induction for 1 h with the combination of both forces showed the presence of the neural precursor nestin as early as 9 h in culture after treatment and this result lasted for the following 6 d. In conclusion, the use of a combination of FSS and EMF for a short-time renders in neurite-like cells, although further investigation is required to analyze cell functionality.
a Cinvestav Monterrey, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav) , Monterrey , México.
Juan Uriel
JU
Mascotte-Cruz
a Cinvestav Monterrey, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav) , Monterrey , México.
Amelia
A
Ríos
a Cinvestav Monterrey, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav) , Monterrey , México.
Bruno
B
Escalante
D000818:Animals; D002454:Cell Differentiation / Q000528:radiation effects*; D004574:Electromagnetic Fields / Q000009:adverse effects; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology* / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar; D033081:Shear Strength; D013314:Stress, Mechanical
Extremely low-frequency electromagnetic fields; bone marrow-derived mesenchymal stem cells; fluid shear stress