Effect of 50 Hz Extremely Low-Frequency Electromagnetic Fields on the DNA Methylation and DNA Methyltransferases in Mouse Spermatocyte-Derived Cell Line GC-2.
Previous studies have shown that the male reproductive system is one of the most sensitive organs to electromagnetic radiation. However, the biological effects and molecular mechanism are largely unclear. Our study was designed to elucidate the epigenetic effects of 50 Hz ELF-EMF in vitro. Mouse spermatocyte-derived GC-2 cell line was exposed to 50 Hz ELF-EMF (5 min on and 10 min off) at magnetic field intensity of 1 mT, 2 mT, and 3 mT with an intermittent exposure for 72 h. We found that 50 Hz ELF-EMF exposure decreased genome-wide methylation at 1 mT, but global methylation was higher at 3 mT compared with the controls. The expression of DNMT1 and DNMT3b was decreased at 1 mT, and 50 Hz ELF-EMF can increase the expression of DNMT1 and DNMT3b of GC-2 cells at 3 mT. However, 50 Hz ELF-EMF had little influence on the expression of DNMT3a. Then, we established DNA methylation and gene expression profiling and validated some genes with aberrant DNA methylation and expression at different intensity of 50 Hz ELF-EMF. These results suggest that the alterations of genome-wide methylation and DNMTs expression may play an important role in the biological effects of 50 Hz ELF-EMF exposure.
College of Bioengineering, Chongqing University, Chongqing 400044, China ; Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Yong
Y
Liu
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Wen-bin
WB
Liu
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Kai-jun
KJ
Liu
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Lin
L
Ao
College of Bioengineering, Chongqing University, Chongqing 400044, China.
Julia Li
JL
Zhong
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Jia
J
Cao
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Jin-yi
JY
Liu
D000818:Animals; D002460:Cell Line; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000096:biosynthesis*; D019175:DNA Methylation / Q000528:radiation effects*; D004574:Electromagnetic Fields; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D051379:Mice; D013090:Spermatocytes / Q000201:enzymology / Q000528:radiation effects*; D000097584:DNA Methyltransferase 3B
D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D000097584:DNA Methyltransferase 3B; C000619381:Dnmt1 protein, mouse