MAP kinase phosphatase 3 inhibits brown adipocyte differentiation via regulation of Erk phosphorylation.
Brown fat has been highlight as a new therapeutic target for treatment of obesity and diabetes. However, molecular mechanism underlying brown adipogenesis are not fully understood. Here, we identified that MAP kinase phosphatase 3 (MKP3) has a novel role as regulator of brown adipocyte differentiation. The expression of MKP3 was significantly decreased during the early stage(s) of brown adipocyte differentiation in HIB-1B cells and primary cells. Ectopic expression of MKP3 led to reduced brown adipocyte differentiation, whereas depletion of MKP3 significantly enhanced the differentiation of primary brown preadipocytes. Consistently, we found an increased brown adipocyte differentiation in MKP3-null MEF cells. These inhibitory effects of MKP3 could be resulted via the temporal regulation of Erk activation. In recent, it was reported that MKP3 deficient mice are resistant to diet-induced obesity, and display enhanced energy expenditure. Taken together, we suggest that MKP3 could be an important factor in the regulation of brown adipocyte differentiation.
Functional Genomics Research Center, KRIBB, Daejeon, 305-806, Republic of Korea; Department of Functional Genomics, University of Science and Technology of Korea, Daejeon, 305-806, Republic of Korea.
Won Kon
WK
Kim
Functional Genomics Research Center, KRIBB, Daejeon, 305-806, Republic of Korea.
Kyoung-Jin
KJ
Oh
Functional Genomics Research Center, KRIBB, Daejeon, 305-806, Republic of Korea.
Hye-Ryung
HR
Choi
Functional Genomics Research Center, KRIBB, Daejeon, 305-806, Republic of Korea.
Anna
A
Park
Functional Genomics Research Center, KRIBB, Daejeon, 305-806, Republic of Korea; Department of Functional Genomics, University of Science and Technology of Korea, Daejeon, 305-806, Republic of Korea.
Baek Soo
BS
Han
Functional Genomics Research Center, KRIBB, Daejeon, 305-806, Republic of Korea.
Seung-Wook
SW
Chi
Functional Genomics Research Center, KRIBB, Daejeon, 305-806, Republic of Korea.
Seung Jun
SJ
Kim
Functional Genomics Research Center, KRIBB, Daejeon, 305-806, Republic of Korea; Department of Functional Genomics, University of Science and Technology of Korea, Daejeon, 305-806, Republic of Korea. Electronic address: khbae@kribb.re.kr.
Kwang-Hee
KH
Bae
Functional Genomics Research Center, KRIBB, Daejeon, 305-806, Republic of Korea; Department of Functional Genomics, University of Science and Technology of Korea, Daejeon, 305-806, Republic of Korea. Electronic address: lesach@kribb.re.kr.
Sang Chul
SC
Lee
D016475:3T3 Cells; D052437:Adipocytes, Brown / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis; D002001:Adipose Tissue, Brown / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D054642:Dual Specificity Phosphatase 6 / Q000737:chemistry / Q000378:metabolism*; D004734:Energy Metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000737:chemistry* / Q000378:metabolism; D055785:Gene Knockdown Techniques; D055786:Gene Knockout Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000235:genetics; D010766:Phosphorylation; D061251:Primary Cell Culture
D048049:Extracellular Signal-Regulated MAP Kinases; D054642:Dual Specificity Phosphatase 6; C517036:Dusp6 protein, mouse
Brown adipogenesis; ERK; MKP3; Obesity