Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.
Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.|Department of Orthopedic Surgery, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi Province, China.
Rui
R
Ma
Translational Medicine Research and Development Centre, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, The Chinese Academy of Sciences, Shenzhen, China.
Yu-xiao
YX
Lai
Translational Medicine Research and Development Centre, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, The Chinese Academy of Sciences, Shenzhen, China.
Long
L
Li
Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Hong-lue
HL
Tan
Department of Orthopedics and Traumatology, The Chinese University of Hong Kong, Hong Kong, China.
Jia-li
JL
Wang
Translational Medicine Research and Development Centre, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, The Chinese Academy of Sciences, Shenzhen, China.
Ye
Y
Li
Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Ting-ting
TT
Tang
Translational Medicine Research and Development Centre, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, The Chinese Academy of Sciences, Shenzhen, China.|Department of Orthopedics and Traumatology, The Chinese University of Hong Kong, Hong Kong, China.
Ling
L
Qin
D000818:Animals; D000900:Anti-Bacterial Agents; D001422:Bacterial Adhesion; D018441:Biofilms; D018786:Bone Substitutes / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D019344:Lactic Acid / Q000737:chemistry; D008274:Magnesium / Q000737:chemistry; D051379:Mice; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013211:Staphylococcus aureus / Q000187:drug effects / Q000648:ultrastructure; D054457:Tissue Scaffolds / Q000737:chemistry
D000900:Anti-Bacterial Agents; D018786:Bone Substitutes; D002130:Calcium Phosphates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D008274:Magnesium; C018392:tricalcium phosphate