Ultrafast sonochemical synthesis of protein-inorganic nanoflowers.
We developed a simple but efficient method to synthesize protein-inorganic hybrid nanostructures with a flower-like shape (nanoflowers), which relies on sonication to facilitate the synthesis of the nanoflowers. With this technique, we synthesized nanoflowers containing laccase as a model protein and copper phosphate within 5 minutes at room temperature. The resulting laccase nanoflowers yielded greatly enhanced activity, stability, and reusability, and their usefulness was successfully demonstrated by applying them in the colorimetric detection of epinephrine. The strategy developed could be used to rapidly synthesize nanoflowers for various applications in biosensor and enzyme catalysis and would expand the utilization of nanoflowers in diverse fields of biotechnology.
Department of Chemical and Biomolecular Engineering (BK21+ Program), Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
Bhagwan S
BS
Batule
Department of Chemical and Biomolecular Engineering (BK21+ Program), Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
Ki Soo
KS
Park
Department of BioNano Technology, Gachon University, Seongnam, Republic of Korea.
Moon Il
MI
Kim
Department of Chemical and Biomolecular Engineering (BK21+ Program), Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
Hyun Gyu
HG
Park
D015374:Biosensing Techniques / Q000379:methods; D003124:Colorimetry / Q000379:methods; D003300:Copper / Q000737:chemistry; D004837:Epinephrine / Q000032:analysis; D053761:Nanocomposites / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D013010:Sonication / Q000379:methods*
D011506:Proteins; D003300:Copper; D004837:Epinephrine
copper phosphate; epinephrine; hybrid nanomaterials; laccase