Formosa haliotis sp. nov., a brown-alga-degrading bacterium isolated from the gut of the abalone Haliotis gigantea.
Four brown-alga-degrading, Gram-stain-negative, aerobic, non-flagellated, gliding and rod-shaped bacteria, designated LMG 28520T, LMG 28521, LMG 28522 and LMG 28523, were isolated from the gut of the abalone Haliotis gigantea obtained in Japan. The four isolates had identical random amplified polymorphic DNA patterns and grew optimally at 25 °C, at pH 6.0-9.0 and in the presence of 1.0-4.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences placed the isolates in the genus Formosa with Formosa algae and Formosa arctica as closest neighbours. LMG 28520T and LMG 28522 showed 100 % DNA-DNA relatedness to each other, 16-17 % towards F. algae LMG 28216T and 17-20 % towards F. arctica LMG 28318T; they could be differentiated phenotypically from these established species. The predominant fatty acids of isolates LMG 28520T and LMG 28522 were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C15 : 1 G and iso-C15 : 0. Isolate LMG 28520T contained menaquinone-6 (MK-6) as the major respiratory quinone and phosphatidylethanolamine, two unknown aminolipids and an unknown lipid as the major polar lipids. The DNA G+C content was 34.4 mol% for LMG 28520T and 35.5 mol% for LMG 28522. On the basis of their phylogenetic and genetic distinctiveness, and differential phenotypic properties, the four isolates are considered to represent a novel species of the genus Formosa, for which the name Formosa haliotis sp. nov. is proposed. The type strain is LMG 28520T ( = NBRC 111189T).
Laboratory of Marine Microbiology, Mie University, Kurima-machiya 1577, Tsu, Mie, 514-8507, Japan.|Japan Science and Technology Agency, CREST, Tokyo, Japan.
Reiji
R
Tanaka
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Faculty of Science, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Ilse
I
Cleenwerck
Laboratory of Marine Microbiology, Mie University, Kurima-machiya 1577, Tsu, Mie, 514-8507, Japan.
Yukino
Y
Mizutani
School of Fisheries and Aquaculture Science, University Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia.
Shunpei
S
Iehata
Laboratory of Marine Microbiology, Mie University, Kurima-machiya 1577, Tsu, Mie, 514-8507, Japan.|Japan Science and Technology Agency, CREST, Tokyo, Japan.
Toshiyuki
T
Shibata
Laboratory of Marine Microbiology, Mie University, Kurima-machiya 1577, Tsu, Mie, 514-8507, Japan.|Japan Science and Technology Agency, CREST, Tokyo, Japan.
Hideo
H
Miyake
Japan Science and Technology Agency, CREST, Tokyo, Japan.|Faculty of Science and Engineering, Waseda University, Wakamatsu, 2-2, Shinjyuku, 162-8480, Japan.
Tetsushi
T
Mori
Laboratory of Marine Microbiology, Mie University, Kurima-machiya 1577, Tsu, Mie, 514-8507, Japan.
Yutaka
Y
Tamaru
Japan Science and Technology Agency, CREST, Tokyo, Japan.|Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwake, Sakyo, Kyoto, 606-8502, Japan.
Mitsuyoshi
M
Ueda
Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Rozier 44, B-9000 Ghent, Belgium.
Peter
P
Bossier
Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Peter
P
Vandamme
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D049851:Gastropoda / Q000382:microbiology*; D007564:Japan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D000459:Phaeophyceae; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013624:Taiwan; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6