Using synchrotrons and XFELs for time-resolved X-ray crystallography and solution scattering experiments on biomolecules.
Time-resolved structural information is key to understand the mechanism of biological processes, such as catalysis and signalling. Recent developments in X-ray sources as well as data collection and analysis methods are making routine time-resolved X-ray crystallography and solution scattering experiments a real possibility for structural biologists. Here we review the information that can be obtained from these techniques and discuss the considerations that must be taken into account when designing a time-resolved experiment.
Department of Physics and Chemistry, University of Palermo, Palermo 90128, Italy.
Matteo
M
Levantino
Hamburg Centre for Ultrafast Imaging & Institute of Nanostructure and Solid State Physics, University of Hamburg, Hamburg 22607, Germany.
Briony A
BA
Yorke
Astbury Centre for Structural Molecular Biology & School of Chemistry, University of Leeds, Leeds LS2 9JT, UK.
Diana Cf
DC
Monteiro
Department of Physics, UMR UR1-CNRS 6251, University of Rennes 1, Rennes 35042, France.
Marco
M
Cammarata
Hamburg Centre for Ultrafast Imaging & Institute of Nanostructure and Solid State Physics, University of Hamburg, Hamburg 22607, Germany. Electronic address: arwen.pearson@cfel.de.
Arwen R
AR
Pearson
D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods*; D004583:Electrons; D006801:Humans; D007834:Lasers; D012542:Scattering, Radiation; D012996:Solutions; D017356:Synchrotrons
D012996:Solutions