Effects of sublethal doses of glyphosate on honeybee navigation.
Glyphosate (GLY) is a herbicide that is widely used in agriculture for weed control. Although reports about the impact of GLY in snails, crustaceans and amphibians exist, few studies have investigated its sublethal effects in non-target organisms such as the honeybee Apis mellifera, the main pollen vector in commercial crops. Here, we tested whether exposure to three sublethal concentrations of GLY (2.5, 5 and 10 mg l(-1): corresponding to 0.125, 0.250 and 0.500 μg per animal) affects the homeward flight path of honeybees in an open field. We performed an experiment in which forager honeybees were trained to an artificial feeder, and then captured, fed with sugar solution containing traces of GLY and released from a novel site either once or twice. Their homeward trajectories were tracked using harmonic radar technology. We found that honeybees that had been fed with solution containing 10 mg l(-1) GLY spent more time performing homeward flights than control bees or bees treated with lower concentrations. They also performed more indirect homing flights. Moreover, the proportion of direct homeward flights performed after a second release from the same site increased in control bees but not in treated bees. These results suggest that, in honeybees, exposure to levels of GLY commonly found in agricultural settings impairs the cognitive capacities needed to retrieve and integrate spatial information for a successful return to the hive. Therefore, honeybee navigation is affected by ingesting traces of the most widely used herbicide worldwide, with potential long-term negative consequences for colony foraging success.
Laboratorio de Insectos Sociales, Departamento de Biodiversidad y Biología Experimental, IFIBYNE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria (C1428EHA), Buenos Aires, Argentina.
María Sol
MS
Balbuena
Institut für Biologie, Freie Universität Berlin, Berlin 14195, Germany.
Léa
L
Tison
Institut für Biologie, Freie Universität Berlin, Berlin 14195, Germany.
Marie-Luise
ML
Hahn
Institut für Biologie, Freie Universität Berlin, Berlin 14195, Germany.
Uwe
U
Greggers
Institut für Biologie, Freie Universität Berlin, Berlin 14195, Germany.
Randolf
R
Menzel
Laboratorio de Insectos Sociales, Departamento de Biodiversidad y Biología Experimental, IFIBYNE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria (C1428EHA), Buenos Aires, Argentina walter@fbmc.fcen.uba.ar.
Walter M
WM
Farina
D000818:Animals; D001516:Bees / Q000187:drug effects* / Q000502:physiology; D005426:Flight, Animal / Q000187:drug effects; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D006702:Homing Behavior / Q000187:drug effects*; D008568:Memory / Q000187:drug effects; D011825:Radar; D065854:Spatial Navigation / Q000187:drug effects*; D000097797:Glyphosate
D005998:Glycine; D006540:Herbicides; D000097797:Glyphosate
Apis mellifera; Glyphosate; Harmonic radar tracking; Navigation; Sublethal effects