Signal Amplification Strategy of Triple-Layered Core-Shell Au@Pd@Pt Nanoparticles for Ultrasensitive Immunoassay Detection of Squamous Cell Carcinoma Antigen.
A novel and effective nonenzymatic immunosensor for the sensitive detection of squamous cell carcinoma antigen (SCC- Ag) was described based on triple-layered core-shell Au@Pd@Pt nanoparticles (Au@Pd@Pt NPs). To prepare the immunosensor, primary anti-SCC antibodies (Ab1) were immobilized onto nanoporous gold films (NPGF) of a modified glassy carbon electrode. Au@Pd@Pt NPs that possess strong catalytic activity for the reduction of H2O2 were used as catalytic labels of secondary anti-SCC antibodies (Ab2). Because of the catalytic activities of Au@Pd@Pt NPs and the large surface area of the NPGF, high sensitivity was achieved for the detection of SCC-Ag. The prepared immunosensor showed remarkable results, such as low detection limits (0.6 pg/mL), a wide linear range (0.001-10.0 ng/mL) and high stability and selectivity in the detection of SCC-Ag. Furthermore, the prepared immunosensor exhibited promising properties, which may be useful for real serum sample tests.
Xiaoyue
X
Zhang
Bin
B
Du
Dan
D
Wu
Hongmin
H
Ma
Yong
Y
Zhang
He
H
Li
Qin
Q
Wei
D000951:Antigens, Neoplasm / Q000097:blood*; D015374:Biosensing Techniques; D055088:Early Detection of Cancer / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D057145:Nanoshells; D010165:Palladium / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D012680:Sensitivity and Specificity; D015843:Serpins / Q000097:blood*; D012815:Signal Processing, Computer-Assisted; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000175:diagnosis*
D000951:Antigens, Neoplasm; D015843:Serpins; C051072:squamous cell carcinoma-related antigen; D010984:Platinum; D010165:Palladium; D006046:Gold