Two Effective Routes for Removing Lineage Restriction Roadblocks: From Somatic Cells to Hepatocytes.
The conversion of somatic cells to hepatocytes has fundamentally re-shaped traditional concepts regarding the limited resources for hepatocyte therapy. With the various induced pluripotent stem cell (iPSC) generation routes, most somatic cells can be effectively directed to functional stem cells, and this strategy will supply enough pluripotent material to generate promising functional hepatocytes. However, the major challenges and potential applications of reprogrammed hepatocytes remain under investigation. In this review, we provide a summary of two effective routes including direct reprogramming and indirect reprogramming from somatic cells to hepatocytes and the general potential applications of the resulting hepatocytes. Through these approaches, we are striving toward the goal of achieving a robust, mature source of clinically relevant lineages.
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou 310058, China. 11318093@zju.edu.cn.
Chenxia
C
Hu
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou 310058, China. ljli@zju.edu.cn.
Lanjuan
L
Li
D000818:Animals; D018929:Cell Culture Techniques; D019070:Cell Lineage / Q000235:genetics; D054338:Cell Transdifferentiation; D064987:Cell- and Tissue-Based Therapy; D065150:Cellular Reprogramming; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D057285:Precision Medicine; D033581:Stem Cell Transplantation
differentiation; hepatocyte; in vitro; in vivo; induced pluripotent stem cells; reprogramming