Interleukin-27 Protects Cardiomyocyte-Like H9c2 Cells against Metabolic Syndrome: Role of STAT3 Signaling.
The present results demonstrated that high glucose (G), salt (S), and cholesterol C (either alone or in combination), as mimicking extracellular changes in metabolic syndrome, damage cardiomyocyte-like H9c2 cells and reduce their viability in a time-dependent manner. However, the effects were greatest when cells were exposed to all three agents (GSC). The mRNA of glycoprotein (gp) 130 and WSX-1, both components of the interleukin (IL)-27 receptor, were present in H9c2 cells. Although mRNA expression was not affected by exogenous treatment with IL-27, the expression of gp130 mRNA (but not that of WSX-1 mRNA) was attenuated by GSC. Treatment of IL-27 to H9c2 cells increased activation of signal transducer and activator of transcription 3 (STAT3) and protected cells from GSC-induced cytochrome c release and cell damage. The protective effects of IL-27 were abrogated by the STAT3 inhibitor, stattic. The results of the present study clearly demonstrate that the STAT3 pathway triggered by anti-inflammatory IL-27 plays a role in protecting cardiomyocytes against GSC-mediated damage.
Department of Cardiology, Cardinal Tien Hospital, New Taipei City 23148, Taiwan.
Wei-Lian
WL
Phan
School of Medicine, Fu Jen Catholic University, New Taipei City 24205, Taiwan.
Yu-Tzu
YT
Huang
School of Medicine, Fu Jen Catholic University, New Taipei City 24205, Taiwan.
Ming-Chieh
MC
Ma
D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000494:pharmacology; D045304:Cytochromes c / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D006023:Glycoproteins / Q000096:biosynthesis / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D007378:Interleukins / Q000378:metabolism / Q000494:pharmacology*; D024821:Metabolic Syndrome / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects
D006023:Glycoproteins; C112441:IL27RA protein, human; D007378:Interleukins; C464565:MYDGF protein, human; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C480482:glycoprotein 130, human; D045304:Cytochromes c; D002784:Cholesterol; D005947:Glucose