Antioxidative effects of α-lipoic acid in the brain, liver and kidneys in selected mouse organs exposed to zymosan.
This study investigated the role of exogenous α-lipoic acid (ALA) in the inflammation caused by zymosan application. Seventy-two adult male white mice were divided into twelve groups: three control groups, three Zymosan groups, three ALA groups and three groups being the combination of Zymosan and ALA. In the experimental groups, the animals were decapitated after 3, 6 and 24 hours after the injection. The activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were determined in the brain, liver and kidneys of the mice. After the injection of Zymosan, it was found that the activity of SOD, CAT and GSH-Px in the brain, liver and kidneys of mice was significantly lower in all time periods. The administration of ALA resulted in an opposite effect, namely, it increased the activity of the enzymes studied in the selected organs of mice. The Zymosan and ALA combination significantly inhibited the decrease in the activity of the enzymes compared with the values obtained in the groups of animals which received Zymosan only. The results of our study, using the Zymosan-induced inflammation, clearly indicate that ALA is an anti-inflammatory agent.
Department of Animal Physiology and Toxicology, Institute of Biology, Pedagogical University of Cracow , Podbrzezie 3, 31-054 Cracow , Poland.
Zofia
Z
Goc
Agnieszka
A
Greń
Edyta
E
Kapusta
Karol
K
Dziubek
Waldemar
W
Szaroma
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D010088:Oxidoreductases / Q000378:metabolism; D008063:Thioctic Acid / Q000494:pharmacology*; D015054:Zymosan / Q000633:toxicity*
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D008063:Thioctic Acid; D015054:Zymosan; D010088:Oxidoreductases
Superoxide dismutase; Zymosan; catalase; glutathione peroxidase; α-lipoic acid