Evaluation of Simultaneous Transmission of Chikungunya Virus and Dengue Virus Type 2 in Infected Aedes aegypti and Aedes albopictus (Diptera: Culicidae).
The simultaneous transmission of chikungunya virus (CHIKV) and dengue viruses (DENV) has been a major public health concern because of their sympatric distribution and shared mosquito vectors. Groups of Aedes aegypti (L.) and Aedes albopictus (Skuse) were orally infected with 1.5 × 10(5) PFU/ml of CHIKV and 3.2 × 10(6) FFU/ml of DENV-2 simultaneously or separately in inverse orders and evaluated for dissemination and transmission by qRT-PCR. Simultaneous dissemination of both viruses was detected for all groups in Ae. aegypti and Ae. albopictus while cotransmission of CHIKV and DENV-2 only occurred at low rates after sequential but not simultaneous infection.
Joint Vaccine Acquisition Program, Medical Countermeasure Systems, United States Army, 1564 Freedman Drive, Fort Detrick, MD 21702.
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JT
Nuckols
Biosecurity Research Institute, Kansas State University, 1041 Pat Roberts Hall, Manhattan KS 66506. Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, L-222 Mosier Hall, Manhattan KS 66506.
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YJ
Huang
Biosecurity Research Institute, Kansas State University, 1041 Pat Roberts Hall, Manhattan KS 66506. Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, L-222 Mosier Hall, Manhattan KS 66506.
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Higgs
Department of Pediatrics, University of Texas Medical Branch, 301 University Blvd., Galveston TX 77555-0436. Assay Development Services Division, Galveston National Laboratory, University of Texas Medical Branch, 301 University Blvd., Galveston TX 77555-0610.
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Miller
Department of Pediatrics, University of Texas Medical Branch, 301 University Blvd., Galveston TX 77555-0436. Assay Development Services Division, Galveston National Laboratory, University of Texas Medical Branch, 301 University Blvd., Galveston TX 77555-0610.
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RB
Pyles
Department of Preventative Medicine and Community Health, University of Texas Medical Branch, 301 University Blvd., Galveston TX 77555-1157.
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HM
Spratt
Biosecurity Research Institute, Kansas State University, 1041 Pat Roberts Hall, Manhattan KS 66506.
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Horne
Biosecurity Research Institute, Kansas State University, 1041 Pat Roberts Hall, Manhattan KS 66506. Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, L-222 Mosier Hall, Manhattan KS 66506. dlvanlan@bri.ksu.edu.
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Vanlandingham
D000330:Aedes / Q000821:virology*; D000818:Animals; D065632:Chikungunya Fever / Q000635:transmission* / Q000821:virology; D002646:Chikungunya virus / Q000502:physiology*; D006224:Cricetinae; D003715:Dengue / Q000635:transmission* / Q000821:virology; D003716:Dengue Virus / Q000502:physiology*; D005260:Female; D007303:Insect Vectors / Q000821:virology*
Aedes aegypti; Aedes albopictus; chikungunya; dengue; transmission