[Platelet-derived growth factor-BB induces phenotypic transformation of corpus cavernosum smooth muscle cells in SD rats].
OBJECTIVE
To evaluate the effect of the platelet-derived growth factor-BB (PDGF-BB) on the phenotypic transformation of corpus cavernosum smooth muscle cells (CCSMC) in SD rats.


METHODS
CCSMCs were primarily cultured in the modified tissue sticking medium and subjected to immunofluorescence assay. The cells were divided into a blank control and four PDGF-BB groups, the latter exposed to 5, 10, 20, and 40 ng/ml of PDGF-BB, respectively, for 24 hours, and the cells in the 20 ng/ml PDGF-BB group treated for 24, 48, and 72 hours. The the relative expressions of α-SMA, SMMHC, calponin, and OPN mRNA were determined by real-time fluorescence quantitative RT-PCR (qRT-PCR).


RESULTS
The α-SMA positive rate of the CCSMCs was over 95%. Compared with the blank control group, the expression levels of α-SMA, SMMHC, and calponin mRNA were significantly decreased (P < 0.05) while that of OPN mRNA remarkably increased (P < 0.05) in the PDGF-BB groups. The 20 ng/ml PDGF-BB group also showed significantly downregulated expressions of α-SMA, SMMHC, and calponin mRNA (P < 0.05) and upregulated expression of OPN mRNA (P < 0.05) at 24, 48, and 72 hours.


CONCLUSIONS
PDGF-BB can induce the transformation of the phenotype of CCSMCs in SD rats from the contractile to the synthetic type.
Jin-tai
JT
Luo
Wen-jun
WJ
Yu
An-yang
AY
Wei
Guo-hua
GH
Zeng
D000199:Actins / Q000378:metabolism; D000818:Animals; D000077214:Becaplermin; D002135:Calcium-Binding Proteins / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D009119:Muscle Contraction; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000378:metabolism; D010413:Penis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010641:Phenotype; D020574:Proto-Oncogene Proteins c-sis / Q000008:administration & dosage / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D000096985:Calponins
D000199:Actins; D000077214:Becaplermin; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D018995:Myosin Heavy Chains; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D000096985:Calponins; C541116:ACTA2 protein, human; C509903:MYH11 protein, human