The IFN-β 1b effect on Cu Zn superoxide dismutase (SOD1) in peripheral mononuclear blood cells of relapsing-remitting multiple sclerosis patients and in neuroblastoma SK-N-BE cells.
Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease leading to axonal injury. Even if the etiology of MS is still unknown the disease begins with inflammation involving autoreactive T lymphocytes activation in genetically susceptible subjects. Interferon beta-1b (IFN β 1b) is one of the most used drug in the MS therapy. The results obtained in this study show that the concentration of SOD1 in CSF of relapsing-remitting MS (RR-MS) patients, evaluated by enzyme-linked immunosorbent assay (ELISA), is decreased compared to pathological controls. Moreover, the Western blotting analysis demonstrated that SOD1 in human peripheral blood mononuclear cells (PBMC) in healthy controls was significantly higher compared to MS subjects before starting DMT therapy. In addition IFN β 1b therapy causes an increase of intracellular SOD1 protein as well as mRNA levels in PBMC. Moreover, the treatment of neuroblastoma SK-N-BE cells with IFN β 1b increased SOD1 protein and mRNA levels; these data also suggest that neuroprotective effect of this physiological molecule is, at least in part, carried out through its effect on SOD1. This study demonstrate that DMT therapy is able to increase SOD1 expression in PBMC of RR-MS patients. Therefore, the effectiveness of DMT therapy can be ascribed, at least in part, to an increased levels of this antioxidant enzyme as further confirmed by in vitro studies in SK-N-BE cells.
Dipartimento di Medicina Clinica e Chirurgia, Unità di Fisiologia, Università degli Studi di Napoli Federico II, Italy.
Simona
S
Damiano
Dipartimento di Medicina Clinica e Chirurgia, Unità di Fisiologia, Università degli Studi di Napoli Federico II, Italy.
Anna
A
Sasso
Dipartimento di Scienze della Vita, Seconda Università di Napoli, Italy.
Bruna
B
De Felice
Dipartimento di Scienze, Università della Basilicata, Italy.
Giuseppe
G
Terrazzano
Dipartimento di Neuroscienze e Scienze Riproduttive ed Odontostomatologiche, Università degli Studi di Napoli Federico II, Italy.
Vincenzo
V
Bresciamorra
Dipartimento di Neuroscienze e Scienze Riproduttive ed Odontostomatologiche, Università degli Studi di Napoli Federico II, Italy.
Antonio
A
Carotenuto
Dipartimento di Neuroscienze e Scienze Riproduttive ed Odontostomatologiche, Università degli Studi di Napoli Federico II, Italy.
Nicola S
NS
Orefice
Dipartimento di Neuroscienze e Scienze Riproduttive ed Odontostomatologiche, Università degli Studi di Napoli Federico II, Italy.
Giuseppe
G
Orefice
Dipartimento di Neuroscienze e Scienze Riproduttive ed Odontostomatologiche, Università degli Studi di Napoli Federico II, Italy.
Giovanni
G
Vacca
Dipartimento di Medicina Clinica e Chirurgia, Unità di Fisiologia, Università degli Studi di Napoli Federico II, Italy.
Annamaria
A
Belfiore
Dipartimento di Medicina Clinica e Chirurgia, Unità di Fisiologia, Università degli Studi di Napoli Federico II, Italy.
Mariarosaria
M
Santillo
Dipartimento di Medicina Clinica e Chirurgia, Unità di Fisiologia, Università degli Studi di Napoli Federico II, Italy. Electronic address: mondola@unina.it.
Paolo
P
Mondola
D000328:Adult; D015153:Blotting, Western; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D000068576:Interferon beta-1b / Q000627:therapeutic use*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology; D009447:Neuroblastoma / Q000188:drug therapy* / Q000201:enzymology; D012333:RNA, Messenger / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood* / Q000378:metabolism*; D000072105:Superoxide Dismutase-1
D012333:RNA, Messenger; C000606290:SOD1 protein, human; D000068576:Interferon beta-1b; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1
Interferon; Peripheral blood mononuclear cells; Relapsing-remitting multiple sclerosis (RR-MS); Superoxide dismutase