Ultrastructure and ssrRNA sequencing of Myxidium amazonense n. sp. a myxosporean parasite of Corydoras melini from the Rio Negro river, Amazonas state, Brazil.
In a survey of myxozoan parasites of ornamental freshwater fish from the Rio Negro river, it was found that seven of 30 (23.3 %) Corydoras melini specimens examined had plasmodia of a new Myxidium species (Myxidium amazonense n. sp.) in the gallbladder. The fish were caught in the Rio Negro river, in the municipality of Santa Isabel do Rio Negro, in the state of Amazonas, Brazil. The plasmodia had a tubular shape, which was organized as a spiral spring with several turns in the gallbladder. The development of the myxospores was asynchronic, with disporic pansporoblasts. Mature myxospores were elongated, with 17.0 ± 0.9 (16.1-17.9) μm in length and 3.7 ± 0.7 (3.0-4.4) μm in width, and lightly arcuate from the valval view, with their bodies tapering slowly until ending in rounded extremities. The valval surface had nine to ten grooves in each valve. The polar capsules, one at either end of the spore, had a length of 5.4 ± 0.5 (4.9-5.9) μm and a width of 3.4 ± 0.6 (2.8-4.0) μm. Ultrastructural analysis showed that the wall of the plasmodia had numerous microvilli-like structures, pinocytotic canals, and cytoplasmic bridges connecting the pansporoblasts to each other and to the ectoplasm zone. Phylogenetic analysis, based on a small subunit ribosomal RNA (ssrRNA), identified the new species as a sister species of Myxidiumceccarelli, the unique South American Myxidium species whose ssrRNA sequence is available in the NCBI database. This study is the first description of Myxidium species in ornamental freshwater fish from Amazon.
Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Caixa Postal 6109, CEP 13083-970, Campinas, SP, Brazil. patrickmathews83@gmail.com.
Patrick D
PD
Mathews
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo (USP), Rua Duque de Caxias Norte, 225, CEP 13635-900, Pirassununga, SP, Brazil. marciamg@usp.br.
Marcia R M
MR
Silva
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo (USP), Rua Duque de Caxias Norte, 225, CEP 13635-900, Pirassununga, SP, Brazil. antomaia@usp.br.
Antônio A M
AA
Maia
Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Caixa Postal 6109, CEP 13083-970, Campinas, SP, Brazil. edapadriano@gmail.com.|Departmento de Ciências Biológicas, Universidade Federal de São Paulo, Rua Professor Arthur Riedel, 275, Jardim Eldorado, 09972-270, Diadema, SP, Brazil. edapadriano@gmail.com.
Edson A
EA
Adriano
D000818:Animals; D001483:Base Sequence; D001938:Brazil; D002397:Catfishes / Q000469:parasitology; D005393:Fish Diseases / Q000469:parasitology*; D005704:Gallbladder / Q000469:parasitology; D008969:Molecular Sequence Data; D055764:Myxozoa / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D010273:Parasitic Diseases, Animal / Q000469:parasitology*; D010802:Phylogeny; D054679:Ribosome Subunits, Small / Q000235:genetics*; D045483:Rivers / Q000469:parasitology; D013170:Spores / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure
Amazon; Fish; Gallbladder; Myxidium; Myxozoa; Systematic