AEG-1 Promotes Metastasis Through Downstream AKR1C2 and NF1 in Liver Cancer.
Liver cancer is one of the most lethal cancers, but our knowledge of the molecular mechanism underlying this process remains insufficient. Through deep sequencing and expression regulation analysis in liver cancer cells, we identified two novel factors, AKR1C2 (positive factor) and NF1 (negative factor), as the AEG-1 downstream players in the process of metastasis in liver cancer. They were experimentally validated to have the capacities of regulating cell migration, cell invasion, cell proliferation, and EMT. Further clinic expression and animal model evidence confirmed their functions. Together, our findings provide a new insight into the pharmaceutical and therapeutic use of AEG-1 and downstream AKR1C2 and NF1.
Minimally Intervention Therapy Center of Liver Diseases and Oncology, Beijing You An Hospital, Capital Medical University, Beijing, China.
Cong
C
Li
Xia
X
Wu
Wei
W
Zhang
Jia
J
Li
Huawei
H
Liu
Ming
M
Hao
Junsong
J
Wang
Honghai
H
Zhang
Gengxia
G
Yang
Meijun
M
Hao
Shoupeng
S
Sheng
Yu
Y
Sun
Jiang
J
Long
Xiongbing
X
Hu
Hanshuo
H
Zhang
Caixia
C
Hu
Li
L
Li
Jiasheng
J
Zheng
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000235:genetics / Q000517:prevention & control / Q000556:secondary; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D008113:Liver Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology / Q000628:therapy; D008175:Lung Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000517:prevention & control / Q000556:secondary; D008565:Membrane Proteins; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D025542:Neurofibromin 1 / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins; D000067492:RNAi Therapeutics; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D023041:Xenograft Model Antitumor Assays; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)
D015815:Cell Adhesion Molecules; D006913:Hydroxysteroid Dehydrogenases; D008565:Membrane Proteins; D025542:Neurofibromin 1; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C483039:MTDH protein, human; C435496:AKR1C2 protein, human; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)