Effect of Lactobacillus salivarius on Th1/Th2 cytokines and the number of spleen CD4⁺ CD25⁺ Foxp3⁺ Treg in asthma Balb/c mouse.
BACKGROUND
Bronchial asthma is a chronic airway inflammatory disease that involves T lymphocytes.


METHODS
In order to explore the effect of Lactobacillus salivarius on Th1/Th2 cytokines and the number of spleen CD4(+) CD25(+) Foxp3(+) Treg in asthma Balb/c mouse, we constructed acute asthma model with ovalbumin to observe the mouse behavior change in Balb/c mice. The expression of GATA-3 mRNA and T-bet mRNA was measured by real-time PCR. The proportion of CD4(+) CD25(+) Foxp3(+) Treg/CD4(+) was determined by flow cytometry.


RESULTS
The results demonstrated that oral gavage with Lactobacillus salivarius before sensitization could alleviate the clinical symptoms, airway hyper-reactivity and airway inflammation in asthma mouse to some extent; Lactobacillus salivarius may improve the imbalance of Th1/Th2 in asthma mouse through increasing the expression of T-bet mRNA at the transcriptional level and inhibiting the expression of GATA-3 mRNA simultaneously.


CONCLUSIONS
CD4(+) CD25(+) Foxp3(+) Treg cells may be involved in the pathogenesis of bronchial asthma, and may be the upstream regulatory mechanism of the improvement of Th1/Th2 imbalance by Lactobacillus salivarius.
Department of Pediatric Pulmonology, Shengjing Hospital of China Medical University Shenyang 110004, Liaoning, China.
Xiang
X
Yun
Department of Pediatric Pulmonology, Shengjing Hospital of China Medical University Shenyang 110004, Liaoning, China.
Yunxiao
Y
Shang
Department of Pediatric Pulmonology, Shengjing Hospital of China Medical University Shenyang 110004, Liaoning, China.
Miao
M
Li
D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000382:microbiology* / Q000628:therapy; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D006801:Humans; D007778:Lactobacillus / Q000502:physiology*; D008168:Lung / Q000382:microbiology / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000009:adverse effects; D012333:RNA, Messenger; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000382:microbiology*; D018417:Th1 Cells / Q000382:microbiology; D018418:Th2 Cells / Q000382:microbiology
D016207:Cytokines; D012333:RNA, Messenger; D010047:Ovalbumin
GATA-3; Lactobacillus salivarius; T-bet; asthma; helper T cells; regulatory T cells