[Angiopoietin-like 4 modulates aldolase A expression in human melanoma cell in a PKC dependent manner].
OBJECTIVE
To investigate the association between angiopoietin-like 4 (ANGPTL4) and aldolase A (ALDOA) in human melanoma cell.


METHODS
Overexpression or knockdown of ANGPTL4 was performed in WM-115 or WM-266-4 cells, respectively. The expression of ANGPTL4 and ALDOA was measured by RT-PCR and Western blot, respectively. The promoter activity of ALDOA gene was determined by luciferase assay.


RESULTS
The promoter activity of ALDOA gene and the expression of ALDOA (mRNA and protein) were increased or decreased in the melanoma cells with overexpression or knockdown of ANGPTL4, which was blocked by selective protein kinase C (PKC) inhibitor or restored by PKC agonist, respectively.


CONCLUSIONS
ANGPTL4 promotes ALDOA expression in human melanoma cell in a PKC dependent manner.
Department of Burn and Plastic Surgery, Second Xiangya Hospital, Central South University, Changsha 410013, China.
Yang
Y
Sun
Department of Burn and Plastic Surgery, Second Xiangya Hospital, Central South University, Changsha 410013, China.
Xiancheng
X
Wang
Department of Burn and Plastic Surgery, Second Xiangya Hospital, Central South University, Changsha 410013, China.
Borong
B
Fang
Department of Burn and Plastic Surgery, Second Xiangya Hospital, Central South University, Changsha 410013, China.
Xiang
X
Xiong
Department of Plastic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
Jianhong
J
Long
D000075803:Angiopoietin-Like Protein 4; D042682:Angiopoietins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
C474772:ANGPTL4 protein, human; D000075803:Angiopoietin-Like Protein 4; D042682:Angiopoietins; D012333:RNA, Messenger; D005634:Fructose-Bisphosphate Aldolase