HLA Haplotypes and Genotypes Frequencies in Brazilian Chronic Periodontitis Patients.
Human leukocyte antigens (HLA) have a pivotal role in immune response and may be involved in antigen recognition of periodontal pathogens. However, the associations of HLA with chronic periodontitis (CP) have not been previously studied in the Brazilian population. In an attempt to clarify the issue of genetic predisposition to CP, we examined the distribution of HLA alleles, genotypes, and haplotypes in patients from Southern Brazil. One hundred and eight CP patients and 151 healthy and unrelated controls with age-, gender-, and ethnicity-matched were HLA investigated by polymerase chain reaction with sequence specific oligonucleotides. To exclude smoking as a predisposing factor, statistical analyses were performed in the total sample and in nonsmoking individuals. The significant results showed a positive association of the A∗ 02/HLA-B∗ 40 haplotype with CP (total samples: 4.2% versus 0%, Pc = 0.03; nonsmokers: 4.3% versus 0%, Pc = 0.23) and a lower frequency of HLA-B∗ 15/HLA-DRB1∗ 11 haplotype in CP compared to controls (total samples: 0.0% versus 4.3%, Pc = 0.04; nonsmokers: 0 versus 5.1%, P = 1.0). In conclusion, the HLA-A∗ 02/B∗ 40 haplotype may contribute to the development of CP, while HLA-B∗ 15/DRB1∗ 11 haplotype might indicate resistance to disease among Brazilians.
Post Graduation Program of Biosciences Applied to Pharmacy, Biomedicine and Clinical Analysis Department, Maringa State University, 87020900 Maringá, PR, Brazil.
Emília Ângela
EÂ
Sippert
Dentistry Department, Maringa State University, 87020900 Maringá, PR, Brazil.
Cléverson
C
de Oliveira e Silva
Post Graduation Program of Biosciences Applied to Pharmacy, Biomedicine and Clinical Analysis Department, Maringa State University, 87020900 Maringá, PR, Brazil.
Christiane Maria
CM
Ayo
Hemocentro of Campinas, HLA Laboratory, University of Campinas, 13083878 Campinas, SP, Brazil.
Silvia Barbosa Dutra
SB
Marques
Basic Health Sciences Department, Maringa State University, 87020900 Maringá, PR, Brazil.
Jeane Eliete Laguila
JE
Visentainer
Basic Health Sciences Department, Maringa State University, 87020900 Maringá, PR, Brazil.
Ana Maria
AM
Sell
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001938:Brazil; D055113:Chronic Periodontitis / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006680:HLA Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains / Q000235:genetics; D059811:HLA-DRB1 Chains / Q000235:genetics; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged
D006680:HLA Antigens; D015235:HLA-B Antigens; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; D059811:HLA-DRB1 Chains