Using headspace solid-phase microextraction for comparison of volatile sulphur compounds of fresh plants belonging to families Alliaceae and Brassicaceae.
In this study, an optimisation of extraction of sulphur volatile compounds (SVCs) has been performed using Central Composite Design. The conditions of the highest amount of eluated peaks and total peaks area have been treated. Factors such as coating of fiber for SPME (Solid Phase Microextraction), extraction temperature and extraction time have been optimised. The SVCs have shown the optimal extraction using a DVB/CAR/PDMS (divinylbenzene/carboxen/polydimethylsiloxane) fiber at 73 °C during 50 min. Furthermore, a pre-incubation step lasting 20 min at the extraction temperature has been used. In total, 12 samples have been investigated at the mentioned optimal conditions, eight from the Alliaceae and four from the Brassicaceae family. The highest number of SVCs (24) has been identified in the sample of chive. The most frequently identified compound found in 11 of 12 samples has been dimethyl trisulphide.
Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic.
Daniel
D
Kremr
Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic.
Petra
P
Bajerová
Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic.
Tomáš
T
Bajer
Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic.
Aleš
A
Eisner
Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic.
Martin
M
Adam
Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic.
Karel
K
Ventura
Alliaceae; Brassicaceae; Gas chromatography/mass spectrometry; Headspace; Solid-phase microextraction; Sulphur volatile compounds