Light-Directed Synthesis of High-Density Peptide Nucleic Acid Microarrays.
Peptide nucleic acids (PNAs) are a class of nucleic acid mimics that can bind to the complementary DNA or RNA with high specificity and sensitivity. PNA-based microarrays have distinct characteristics and have improved performance in many aspects compared to DNA microarrays. A new set of PNA monomers has been synthesized and used as the building blocks for the preparation of high density PNA microarrays. These monomers have their backbones protected by the photolabile group 2-(2-nitrophenyl)propyloxy carbonyl (NPPOC), and their exocyclic amino groups protected by amide carbonyl groups. A light-directed synthesis system was designed and applied to the in situ synthesis of a PNA microarray with a density of over 10,000 probes per square centimeter. This PNA microarray was able to detect single and multiple base-mismatches correctly with a high discrimination ratio.
Department of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University , Changsha, 410083, China.
Feipeng
F
Yang
Department of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University , Changsha, 410083, China.
Bo
B
Dong
Department of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University , Changsha, 410083, China.
Kaixuan
K
Nie
Department of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University , Changsha, 410083, China.
Huanhuan
H
Shi
Department of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University , Changsha, 410083, China.
Yanqi
Y
Wu
Department of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University , Changsha, 410083, China.
Hongyin
H
Wang
Department of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University , Changsha, 410083, China.
Zhengchun
Z
Liu
D007202:Indicators and Reagents; D008027:Light; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis*; D055668:Photochemical Processes; D014466:Ultraviolet Rays
D007202:Indicators and Reagents; D020135:Peptide Nucleic Acids
NPPOC; microarray; monomer; peptide nucleic acid; photodeprotection