Ring-Closing and Cross-Metathesis with Artificial Metalloenzymes Created by Covalent Active Site-Directed Hybridization of a Lipase.
A series of Grubbs-type catalysts that contain lipase-inhibiting phosphoester functionalities have been synthesized and reacted with the lipase cutinase, which leads to artificial metalloenzymes for olefin metathesis. The resulting hybrids comprise the organometallic fragment that is covalently bound to the active amino acid residue of the enzyme host in an orthogonal orientation. Differences in reactivity as well as accessibility of the active site by the functionalized inhibitor became evident through variation of the anchoring motif and substituents on the N-heterocyclic carbene ligand. Such observations led to the design of a hybrid that is active in the ring-closing metathesis and the cross-metathesis of N,N-diallyl-p-toluenesulfonamide and allylbenzene, respectively, the latter being the first example of its kind in the field of artificial metalloenzymes.
Organic Chemistry and Catalysis, Institute for Nanomaterials Science, Faculty of Science, Utrecht University, Universiteitsweg 99, Utrecht 3584CG (The Netherlands).
Manuel
M
Basauri-Molina
Organic Chemistry and Catalysis, Institute for Nanomaterials Science, Faculty of Science, Utrecht University, Universiteitsweg 99, Utrecht 3584CG (The Netherlands).
Dide G A
DG
Verhoeven
Organic Chemistry and Catalysis, Institute for Nanomaterials Science, Faculty of Science, Utrecht University, Universiteitsweg 99, Utrecht 3584CG (The Netherlands).
Arnoldus J
AJ
van Schaik
Organic Chemistry and Catalysis, Institute for Nanomaterials Science, Faculty of Science, Utrecht University, Universiteitsweg 99, Utrecht 3584CG (The Netherlands).
Henk
H
Kleijn
Organic Chemistry and Catalysis, Institute for Nanomaterials Science, Faculty of Science, Utrecht University, Universiteitsweg 99, Utrecht 3584CG (The Netherlands). r.j.m.kleingebbink@uu.nl.
Robertus J M
RJ
Klein Gebbink
D000475:Alkenes / Q000737:chemistry*; D000498:Allyl Compounds / Q000737:chemistry*; D001555:Benzene Derivatives / Q000737:chemistry*; D040761:Biomimetic Materials; D002384:Catalysis; D020134:Catalytic Domain; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D008667:Metalloproteins / Q000737:chemistry* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012428:Ruthenium / Q000737:chemistry*; D013237:Stereoisomerism; D000081248:Allylbenzene Derivatives
D000475:Alkenes; D000498:Allyl Compounds; D001555:Benzene Derivatives; D008049:Lipase; D008667:Metalloproteins; D012428:Ruthenium; C102347:allylbenzene; D000081248:Allylbenzene Derivatives
enzyme catalysis; lipase; metalloenzymes; metathesis; ruthenium