Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster.
Copper oxide nanoparticles (CuONPs) are used as semiconductors, catalysts, gas sensors, and antimicrobial agents. We have used the comet and wing-spot assays in Drosophila melanogaster to assess the genotoxicity of CuONPs and ionic copper (CuSO4). Lipid peroxidation analysis was also performed (Thiobarbituric Acid Assay, TBARS). In larval hemocytes, both CuONPs and CuSO4 caused significant dose-dependent increases in DNA damage (comet assay). In the wing-spot assay, an increase in the frequency of mutant spots was observed in the wings of the adults; CuONPs were more effective than was CuSO4. Both agents induced TBARS; again, CuONPs were more active than was CuSO4. The results indicate that CuONPs are genotoxic in Drosophila, and these effects may be mediated by oxidative stress. Most of the effects appear to be related to the presence of copper ions.
Núcleo de Investigación en Estudios Ambientales, Facultad de Recursos Naturales, Escuela de Medicina Veterinaria, Grupo de Genotoxicología, Universidad Católica de Temuco, Temuco, Chile. Electronic address: ecarmona@uct.cl.
Erico R
ER
Carmona
Núcleo de Investigación en Producción Alimentaria, Facultad de Recursos Naturales, Escuela de Agronomía, Universidad Católica de Temuco, Temuco, Chile.
Claudio
C
Inostroza-Blancheteau
Núcleo de Investigación en Estudios Ambientales, Facultad de Recursos Naturales, Escuela de Medicina Veterinaria, Grupo de Genotoxicología, Universidad Católica de Temuco, Temuco, Chile.
Veroska
V
Obando
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain.
Laura
L
Rubio
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain; CIBER Epidemiología y Salud Pública, Instituto de Salud Carlos III, Madrid, Spain.
Ricard
R
Marcos
D000818:Animals; D020552:Comet Assay / Q000379:methods; D003300:Copper / Q000633:toxicity*; D019327:Copper Sulfate / Q000633:toxicity; D053960:DNA Breaks; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000187:drug effects* / Q000235:genetics; D007814:Larva / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D053768:Metal Nanoparticles / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D014921:Wings, Animal / Q000187:drug effects*
D009153:Mutagens; D003300:Copper; D019327:Copper Sulfate; C030973:cupric oxide
Comet assay; DNA damage; Hemocytes; Malondialdehyde; Mitotic recombination; Wing-spot test