Clinical subsets associated with different anti-aminoacyl transfer RNA synthetase antibodies and their association with coexisting anti-Ro52.
OBJECTIVE
To characterize clinical features of polymyositis/dermatomyositis (PM/DM) patients with different anti-aminoacyl transfer RNA synthetase (ARS) antibodies and their association with anti-Ro52.


METHODS
Autoantibodies in sera from 97 Japanese patients (36 PM, 56 DM, and 5 clinically amyopathic DM), who satisfied Bohan and Peter or modified Sontheimer's criteria, were characterized by immunoprecipitation and enzyme-linked immunosorbent assay. Clinical information was from medical records. Features associated with different anti-ARS and anti-Ro52 antibodies were analyzed.


RESULTS
The prevalence of anti-ARS was similar to other studies (Jo-1, 22%; EJ, 4%; OJ, 1%; PL-12, 1%), except for a high prevalence of anti-PL-7 (12%), which allowed us to characterize patients carrying this specificity. Serum creatine kinase >3000 IU/l was less common in anti-PL-7-positive patients (57%) than anti-Jo-1-positive patients (18%) (p = 0.0328) and was not found in anti-EJ-positive individuals. Interstitial lung disease was common in anti-ARS-positive patients (97%) (p < 0.0001 vs. 48% in anti-ARS-negative). Anti-Ro52 antibodies were frequently detected with anti-ARS (59%) (57% in anti-Jo-1, 67% in anti-PL-7) (vs. 21% in anti-ARS-negative, p < 0.0002). Anti-Ro52 was associated with overlap syndrome (26%) (vs. 7% in anti-Ro52-negative, p = 0.0119).


CONCLUSIONS
Patients with different anti-ARS in combination with anti-Ro52 appear to be associated with distinctive clinical subsets.
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Yoshioki
Y
Yamasaki
b Department of Clinical Nursing, School of Health Sciences , University of Occupational and Environmental Health , Kitakyushu , Japan .|c Division of Rheumatology and Clinical Immunology, Department of Medicine , University of Florida , Gainesville , FL , USA , and.
Minoru
M
Satoh
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Machiko
M
Mizushima
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Takahiro
T
Okazaki
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Hiroko
H
Nagafuchi
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Seido
S
Ooka
d Division of Rheumatology, Department of Internal Medicine , Yokohama City Seibu Hospital, St. Marianna University School of Medicine , Yokohama , Japan.
Tomohiko
T
Shibata
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Hiromasa
H
Nakano
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Hitoshi
H
Ogawa
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Kohei
K
Azuma
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Akihiko
A
Maeda
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Kumiko
K
Tonooka
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Hiroshi
H
Ito
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Yukiko
Y
Takakuwa
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Makoto
M
Inoue
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Hirofumi
H
Mitomi
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Tomofumi
T
Kiyokawa
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Kosei
K
Tsuchida
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Hiromi
H
Matsushita
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Hidenori
H
Mikage
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Yoshihiko
Y
Murakami
c Division of Rheumatology and Clinical Immunology, Department of Medicine , University of Florida , Gainesville , FL , USA , and.
Jason Y F
JY
Chan
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Shoichi
S
Ozaki
a Division of Rheumatology and Allergology, Department of Internal Medicine , St. Marianna University School of Medicine , Kawasaki , Japan .
Hidehiro
H
Yamada
D000328:Adult; D000368:Aged; D000604:Amino Acyl-tRNA Synthetases / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000097:blood / Q000276:immunology*; D003882:Dermatomyositis / Q000097:blood / Q000150:complications / Q000276:immunology*; D005260:Female; D006801:Humans; D017563:Lung Diseases, Interstitial / Q000150:complications; D008297:Male; D008875:Middle Aged; D012261:Ribonucleoproteins / Q000276:immunology*; D000099207:SS-A Antigen
D000604:Amino Acyl-tRNA Synthetases; D001323:Autoantibodies; D012261:Ribonucleoproteins; D000099207:SS-A Antigen
Amyopathic dermatomyositis; Anti-PL-7 antibodies; Anti-Ro52 antibodies; Anti-aminoacyl transfer RNA synthetase (ARS) antibodies; Polymyositis/dermatomyositis