Enhancing saccharification of wheat straw by mixing enzymes from genetically-modified Trichoderma reesei and Aspergillus niger.
OBJECTIVE
To increase the efficiency of enzymatic hydrolysis for plant biomass conversion into renewable biofuel and chemicals.


RESULTS
By overexpressing the point mutation A824 V transcriptional activator Xyr1 in Trichoderma reesei, carboxymethyl cellulase, cellobiosidase and β-D-glucosidase activities of the best mutant were increased from 1.8 IU/ml, 0.1 IU/ml and 0.05 IU/ml to 4.8 IU/ml, 0.4 IU/ml and 0.3 IU/ml, respectively. The sugar yield of wheat straw saccharification by combining enzymes from this mutant and the Aspergillus niger genetically modified strain ΔcreA/xlnR c/araR c was improved up to 7.5 mg/ml, a 229 % increase compared to the combination of wild type strains.


CONCLUSIONS
Mixing enzymes from T. reesei and A. niger combined with the genetic modification of transcription factors is a promising strategy to increase saccharification efficiency.
Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.
Yanping
Y
Jiang
CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.|Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
Alexandra Vivas
AV
Duarte
CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.|Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
Joost
J
van den Brink
CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.|Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
Ad
A
Wiebenga
Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.
Gen
G
Zou
Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.
Chengshu
C
Wang
CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.|Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
Ronald P
RP
de Vries
Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.
Zhihua
Z
Zhou
CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands. i.benoit@cbs.knaw.nl.|Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands. i.benoit@cbs.knaw.nl.
Isabelle
I
Benoit
D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics; D018533:Biomass; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006868:Hydrolysis; D009154:Mutation; D030781:Organisms, Genetically Modified; D031786:Saccharum / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics; D014908:Triticum / Q000737:chemistry
D005656:Fungal Proteins
Aspergillus niger; Genetically-modified strains; Saccharification; Trichoderma reesei; Wheat straw