[Construction of Producers of Cellulolytic and Pectinolytic Enzymes Based on the Fungus Penicillium verruculosum].
Based on the fungus Penicillium verruculosum, we created strains with a complex of extracellular enzymes that contains both cellulolytic enzymes of the fungus and heterologous pectin lyase A from P. canescens and endo- 1,4-α-polygalacturonase from Aspergillus niger. The endopolygalacturonase and pectin lyase activities of enzyme preparations obtained from culture media of the producer strains reached 46-53 U/mg of protein and 1.3-2.3 U/mg of protein, respectively. The optimal temperature and pH values for recombinant pectin lyase and endopolygalacturonase corresponded to those described in the literature for these enzymes. The content of heterologous endopolygalacturonase and pectin lyase in the studied enzyme preparations was 4-5% and 23% of the total protein content, respectively. The yield of reducing sugars upon the hydrolysis of sugar beet and apple processing wastes with the most efficient preparation was 41 and 71 g/L, respectively, which corresponded to a polysaccharide conversion of 49% and 65%. Glucose was the main product of the hydrolysis of sugar beet and apple processing wastes.
E V
EV
Bushina
E A
EA
Rubtsova
A M
AM
Rozhkova
O A
OA
Sinitsyna
A V
AV
Koshelev
V Yu
VY
Matys
V A
VA
Nemashkalov
A P
AP
Sinitsyn
D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics; D027461:Beta vulgaris / Q000737:chemistry; D005947:Glucose / Q000096:biosynthesis / Q000737:chemistry; D006868:Hydrolysis; D027845:Malus / Q000737:chemistry; D060847:Metabolic Engineering; D010368:Pectins / Q000096:biosynthesis / Q000737:chemistry; D010407:Penicillium / Q000201:enzymology / Q000235:genetics*; D011096:Polygalacturonase / Q000235:genetics* / Q000378:metabolism; D011133:Polysaccharide-Lyases / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins; D010368:Pectins; D011096:Polygalacturonase; D011133:Polysaccharide-Lyases; C027349:pectin lyase; D005947:Glucose