Antithrombotic activity of Vitis labrusca extract on rat platelet aggregation.
Vitis labrusca is a grapevine that has antioxidant, neuroprotective, hepatoprotective, and anticarcinogenic activity. However, the antithrombotic effect of Vitis labrusca leaves on platelets is yet to be ascertained. We investigated the inhibitory effect of V. labrusca leaf extract (VLE) on platelet aggregation in vitro and ex vivo. The thromboxane B2 (TXB2) and serotonin concentrations were measured by ELISA. The flavonoids content was measured by ultraperformance liquid chromatography (UPLC). The antithrombotic activity of VLE was evaluated using various agonists in vitro. VLE strongly inhibited adenosine diphosphate (ADP)-induced platelet aggregation. In rats, VLE treatment (100 mg/kg) reduced ADP-stimulated platelet aggregation, without affecting tail bleeding and coagulation time. Moreover, VLE significantly suppressed TXB2 and serotonin secretion. UPLC analysis indicated that VLE contains quercetin, isorhamnetin, and rutin. Our results indicate that VLE possesses antiplatelet activity via the suppression of TXB2 and serotonin, without affecting bleeding. Further, we identified the flavonoids present in VLE. Thus, VLE may be a potential agent for the prevention of cardiovascular diseases.
aDepartment of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute bDepartment of Food Industry Convergence, Wonkwang University, Iksan cHanpoong Pharm & Foods Co., Ltd. Guretdeul3-gil, Deokjin-gu dDepartment of Immunology and Institute of Medical Science, Jeonbuk National University Medical School, Jeonju, Jeonbuk, Korea.
Se-Uk
SU
Kwon
Hoon-Yeon
HY
Lee
Mingjie
M
Xin
Su-Jeong
SJ
Ji
Hyoung-Kwon
HK
Cho
Dae-Sung
DS
Kim
Dae-Ki
DK
Kim
Young-Mi
YM
Lee
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism; D013929:Thromboxane B2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D027843:Vitis / Q000737:chemistry*
D005419:Flavonoids; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D012701:Serotonin; D013929:Thromboxane B2; D000244:Adenosine Diphosphate