Ultrastructural Analysis of In Vivo Hypoglycemiant Effect of Two Polyoxometalates in Rats with Streptozotocin-Induced Diabetes.
Two polyoxometalates (POMs), synthesized through a self-assembling method, were used in the treatment of streptozotocin (STZ)-induced diabetic rats. One of these nanocompounds [tris(vanadyl)-substituted tungsto-antimonate(III)-anions—POM1] was previously described in the literature, whereas the second [tris-butyltin-21-tungsto-9-antimonate(III)-anions—POM2], was prepared by us based on our original formula. In rats with STZ-induced diabetes treated with POMs (up to a cumulative dose of 4 mg/kg bodyweight at the end of the treatments), statistically significant reduced levels of blood glucose were measured after 3 weeks, as compared with the diabetic control groups (DCGs). Ultrastructural analysis of pancreatic β-cells (including the mean diameter of secretory vesicles and of their insulin granules) in the treated diabetic rats proved the POMs contribute to limitation of cellular degeneration triggered by STZ, as well as to the presence of increased amounts of insulin-containing vesicles as compared with the DCG. The two POMs also showed hepatoprotective properties when ultrastructural aspects of hepatocytes in the experimental groups of rats were studied. Based on our in vivo studies, we concluded that the two POMs tested achieved hypoglycemiant effects by preventing STZ-triggered apoptosis of pancreatic β-cells and stimulation of insulin synthesis.
1Department of Cell and Molecular Biology, Faculty of Medicine,"Iuliu Haţieganu" University of Medicine and Pharmacy,6 Louis Pasteur St.,400349 Cluj-Napoca,România.
Ştefana
Ş
Bâlici
1Department of Cell and Molecular Biology, Faculty of Medicine,"Iuliu Haţieganu" University of Medicine and Pharmacy,6 Louis Pasteur St.,400349 Cluj-Napoca,România.
Modeste
M
Wankeu-Nya
4Department of Physical-Chemistry, Faculty of Pharmacy,"Iuliu Haţieganu" University of Medicine and Pharmacy,6 Louis Pasteur St.,400349 Cluj-Napoca,România.
Dan
D
Rusu
1Department of Cell and Molecular Biology, Faculty of Medicine,"Iuliu Haţieganu" University of Medicine and Pharmacy,6 Louis Pasteur St.,400349 Cluj-Napoca,România.
Gheorghe Z
GZ
Nicula
2Department of Inorganic Chemistry, Faculty of Chemistry and Chemical Engineering,"Babeş-Bolyai" University,11 Arany Janos St.,400028 Cluj-Napoca,România.
Mariana
M
Rusu
1Department of Cell and Molecular Biology, Faculty of Medicine,"Iuliu Haţieganu" University of Medicine and Pharmacy,6 Louis Pasteur St.,400349 Cluj-Napoca,România.
Adrian
A
Florea
1Department of Cell and Molecular Biology, Faculty of Medicine,"Iuliu Haţieganu" University of Medicine and Pharmacy,6 Louis Pasteur St.,400349 Cluj-Napoca,România.
Horea
H
Matei
D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D022781:Hepatocytes / Q000648:ultrastructure; D007004:Hypoglycemic Agents / Q000008:administration & dosage*; D050417:Insulin-Secreting Cells / Q000648:ultrastructure*; D008297:Male; D015388:Organelles / Q000648:ultrastructure*; D010179:Pancreas / Q000473:pathology*; D017208:Rats, Wistar; D016896:Treatment Outcome; D017973:Tungsten Compounds / Q000008:administration & dosage*; D000099294:Polyoxometalates
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D017973:Tungsten Compounds; C102296:polyoxometalate I; D000099294:Polyoxometalates
diabetes; insulin; pancreatic β-cells; polyoxometalates; transmission electron microscopy