Release of Vesicular Stomatitis Virus Spike Protein G-Pseudotyped Lentivirus from the Host Cell Is Impaired upon Low-Density Lipoprotein Receptor Overexpression.
Production of a vesicular stomatitis virus spike protein G (VSVG)-pseudotyped lentiviral expression vector in HEK293 cells decreased on overexpression of low-density lipoprotein receptor (LDLR) but not that of ICAM1 or TfR1. Reverse transcription-quantitative PCR (RT-qPCR) revealed a reduction in vector RNA as a function of LDLR expression. Decreased syncytium formation suggested diminished surface expression of VSVG. Intracellular VSVG granules colocalized with LDLR, ER-Golgi intermediate compartment protein 53 (ERGIC53), LAMP2, and vimentin but not with GM130 or calnexin, suggesting that VSVG interacts with LDLR within the ERGIC, resulting in rerouting into the aggresome/autophagosome pathway.
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University of Vienna, Vienna Biocenter, Vienna, Austria.
Alexander
A
Otahal
Department of Pathophysiology, Medical University of Vienna, Vienna, Austria.
Renate
R
Fuchs
Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, Al-Abedia Campus, Makkah, Saudi Arabia fallaf@hotmail.com dieter.blaas@meduniwien.ac.at.
Faisal A
FA
Al-Allaf
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University of Vienna, Vienna Biocenter, Vienna, Austria fallaf@hotmail.com dieter.blaas@meduniwien.ac.at.
Dieter
D
Blaas
D015703:Antigens, CD / Q000096:biosynthesis; D001324:Autoantigens / Q000378:metabolism; D037281:Calnexin / Q000378:metabolism; D002460:Cell Line; D015726:Giant Cells / Q000166:cytology; D057809:HEK293 Cells; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D052119:Lysosomal-Associated Membrane Protein 2 / Q000378:metabolism; D037241:Mannose-Binding Lectins / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D008565:Membrane Proteins / Q000378:metabolism; D011973:Receptors, LDL / Q000096:biosynthesis*; D011990:Receptors, Transferrin / Q000096:biosynthesis; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000235:genetics; D057074:Virus Release / Q000502:physiology*
D015703:Antigens, CD; D001324:Autoantigens; C068126:CD71 antigen; C028391:G protein, vesicular stomatitis virus; C097741:Golgin subfamily A member 2; C573420:ICAM1 protein, human; C497811:LAMP2 protein, human; C084004:LMAN1 protein, human; D052119:Lysosomal-Associated Membrane Protein 2; D037241:Mannose-Binding Lectins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011973:Receptors, LDL; D011990:Receptors, Transferrin; D014746:Vimentin; D014759:Viral Envelope Proteins; D018799:Intercellular Adhesion Molecule-1; D037281:Calnexin