Printed paper sensors for serum lactate dehydrogenase using pullulan-based inks to immobilize reagents.
In this study, a paper-based point-of-care (POC) colorimetric biosensor was developed for the detection of lactate dehydrogenase in serum using a nonporous, oxygen impermeable reversibly gelling polysaccharide material based on pullulan. The pullulan could be printed onto paper surfaces along with all required assay reagents, providing a means for high-stability immobilization of all reagents on paper. Serum containing lactate dehydrogenase (LDH) was directly spotted on to the pullulan-coated bioactive paper and provided quantitative colorimetric data that was comparable to that obtained with a conventional plate-reader method. The paper strip was found to be highly stable and could be stored at 4 °C for at least 10 weeks with no loss in performance, as compared to a complete loss in performance within 1 day when the reagents were printed without the stabilizing polysaccharide. The ease of fabrication coupled with the high stability of the printed reagents provides a facile platform for easily manufactured POC sensors.
Biointerfaces Institute, McMaster University , 1280 Main St W, Hamilton, Ontario L8S 4L8, Canada.
Balamurali
B
Kannan
Biointerfaces Institute, McMaster University , 1280 Main St W, Hamilton, Ontario L8S 4L8, Canada.|Department of Chemical Engineering, McMaster University , 1280 Main St W, Hamilton, Ontario L8S 4L7, Canada.
Sana
S
Jahanshahi-Anbuhi
Biointerfaces Institute, McMaster University , 1280 Main St W, Hamilton, Ontario L8S 4L8, Canada.|Department of Chemical Engineering, McMaster University , 1280 Main St W, Hamilton, Ontario L8S 4L7, Canada.
Robert H
RH
Pelton
Biointerfaces Institute, McMaster University , 1280 Main St W, Hamilton, Ontario L8S 4L8, Canada.|Department of Biochemistry & Biomedical Sciences, McMaster University , 1280 Main St W, Hamilton, Ontario L8S 3Z5, Canada.
Yingfu
Y
Li
Biointerfaces Institute, McMaster University , 1280 Main St W, Hamilton, Ontario L8S 4L8, Canada.|Department of Chemical Engineering, McMaster University , 1280 Main St W, Hamilton, Ontario L8S 4L7, Canada.
Carlos D M
CD
Filipe
Biointerfaces Institute, McMaster University , 1280 Main St W, Hamilton, Ontario L8S 4L8, Canada.
John D
JD
Brennan
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D003124:Colorimetry; D005936:Glucans / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry; D007281:Ink; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D010209:Paper; D019095:Point-of-Care Systems; D011327:Printing
D005936:Glucans; D007202:Indicators and Reagents; C009109:pullulan; D007770:L-Lactate Dehydrogenase