Molecular characterization of carnitine palmitoyltransferase IA in Megalobrama amblycephala and effects on its expression of feeding status and dietary lipid and berberine.
Carnitine palmitoyltransferase I (CPT I, EC 2.3.1.21) controls the main regulatory step of fatty acid oxidation, and hence studies of its molecular characterization are useful to understand lipid metabolism in cultured fish. Here, a full-length cDNA coding CPT I was cloned from liver of blunt snout bream Megalobrama amblycephala. This cDNA obtained covered 2499bp with an open reading frame of 2181bp encoding 726 amino acids. This CPT I mRNA predominantly expressed in heart and white muscle, while little in eye and spleen. The phylogenetic tree constructed on the basis of sequence alignments among several vertebrate species suggests that this blunt snout bream CPT I sequence belongs to the CPT IA family. In order to investigate the characterization of CPT IA mRNA expression, post-prandial experiment and feeding trial were conducted. The results showed that CPT IA mRNA expression was unchanged from 2 to 12h, and then significantly increased at 24h post-feeding in liver and heart. Berberine, an alkaloid, was identified as a promising lipid-lowering drug. In order to elucidate the effect of berberine on CPT I expression, fish were fed for 8 weeks with three diets (low-fat diet (LFD, 5% fat), high-fat diet (HFD, 15% fat), and berberine-supplemented diet (BSD, 15% fat). The results showed that HFD could decrease the expression of CPT IA and PPARα, while BSD increased those expressions.
Xiamen Key Laboratory for Feed Quality Testing and Safety, College of Fisheries, Jimei University, Xiamen 361021, China; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Kang-Le
KL
Lu
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Ding-Dong
DD
Zhang
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Li-Na
LN
Wang
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Wei-Na
WN
Xu
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: wbliu@njau.edu.cn.
Wen-Bin
WB
Liu
D000818:Animals; D001599:Berberine / Q000494:pharmacology*; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics*; D003530:Cyprinidae / Q000235:genetics* / Q000502:physiology*; D004041:Dietary Fats / Q000494:pharmacology*; D004435:Eating; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D010802:Phylogeny; D019518:Postprandial Period / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D004041:Dietary Fats; D012333:RNA, Messenger; D001599:Berberine; D002334:Carnitine O-Palmitoyltransferase
Blunt snout bream; Carnitine palmitoyltransferase I; Feeding status; Gene cloning; mRNA expression