Diabetic Retinopathy: Retina-Specific Methods for Maintenance of Diabetic Rodents and Evaluation of Vascular Histopathology and Molecular Abnormalities.
Diabetic retinopathy is a major cause of visual impairment, which continues to increase in prevalence as more and more people develop diabetes. Despite the importance of vision, the retina is one of the smallest tissues in the body, and specialized techniques have been developed to study retinopathy. This article summarizes several methods used to (i) induce diabetes in mice, (ii) maintain the diabetic animals throughout the months required for development of typical vascular histopathology, (iii) evaluate vascular histopathology of diabetic retinopathy, and (iv) quantitate abnormalities implicated in the development of the retinopathy.
Case Western Reserve University and Case Medical Center, Cleveland, Ohio.|Veterans Administration Medical Center Research Service 151, Cleveland, Ohio.|These authors contributed equally to this work.
Alexander
A
Veenstra
Case Western Reserve University and Case Medical Center, Cleveland, Ohio.|These authors contributed equally to this work.
Haitao
H
Liu
Case Western Reserve University and Case Medical Center, Cleveland, Ohio.
Chieh Allen
CA
Lee
Case Western Reserve University and Case Medical Center, Cleveland, Ohio.
Yunpeng
Y
Du
Case Western Reserve University and Case Medical Center, Cleveland, Ohio.|Veterans Administration Medical Center Research Service 151, Cleveland, Ohio.
Jie
J
Tang
Case Western Reserve University and Case Medical Center, Cleveland, Ohio.|Veterans Administration Medical Center Research Service 151, Cleveland, Ohio.
Timothy S
TS
Kern
D000818:Animals; D003930:Diabetic Retinopathy / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D051381:Rats; D012160:Retina / Q000002:abnormalities / Q000378:metabolism / Q000473:pathology*; D012171:Retinal Vessels / Q000473:pathology*; D013481:Superoxides / Q000378:metabolism; D020858:Tissue and Organ Harvesting / Q000379:methods*
D013481:Superoxides
diabetes; mouse; retina; retinopathy; streptozotocin