Arsenic Trioxide Exposure Induces Heat Shock Protein Responses in Cock Livers.
Arsenic is a trace element widely found in nature, and there are several forms of arsenic, including the most toxic form of trivalent arsenic. Arsenic trioxide (As2O3) is widespread in nature and tends to accumulate in animals and humans, thus causing great harm. Although the important role of heat shock proteins (HSPs) has been demonstrated in many types of mammals exposed to As2O3, the function of these proteins in poultry, especially in cocks, remains unclear. In this study, we used experimental animals (male chickens), which were fed a diet including 0, 7.5, 15, and 30 mg kg(-1) As2O3, respectively, in the control, low, middle, and high groups. The livers were collected after the cocks were treated with arsenic for 30, 60, and 90 days. We detected HSP27, HSP60, HSP70, and HSP90 levels in the livers of the cocks by real-time PCR and HSP60 and HSP70 levels by Western blot. The results showed that the messenger RNA and protein expression of HSPs exposed to As2O3 had obviously increased. These results demonstrated that arsenic toxicity affected the expression of HSP levels in cock livers.
College of Wildlife Resources, Northeast Forestry University, 26 Hexing Road, Harbin, Heilongjiang Province, 150040, China.
Kexin
K
Zhang
College of Wildlife Resources, Northeast Forestry University, 26 Hexing Road, Harbin, Heilongjiang Province, 150040, China.
Panpan
P
Zhao
College of Wildlife Resources, Northeast Forestry University, 26 Hexing Road, Harbin, Heilongjiang Province, 150040, China.
Guangyang
G
Guo
College of Wildlife Resources, Northeast Forestry University, 26 Hexing Road, Harbin, Heilongjiang Province, 150040, China.
Ying
Y
Guo
College of Wildlife Resources, Northeast Forestry University, 26 Hexing Road, Harbin, Heilongjiang Province, 150040, China.
Siwen
S
Li
College of Wildlife Resources, Northeast Forestry University, 26 Hexing Road, Harbin, Heilongjiang Province, 150040, China.
Ying
Y
He
College of Wildlife Resources, Northeast Forestry University, 26 Hexing Road, Harbin, Heilongjiang Province, 150040, China.
Xiao
X
Sun
College of Wildlife Resources, Northeast Forestry University, 26 Hexing Road, Harbin, Heilongjiang Province, 150040, China. 17758625@163.com.
Hongliang
H
Chai
College of Wildlife Resources, Northeast Forestry University, 26 Hexing Road, Harbin, Heilongjiang Province, 150040, China. zhangwenwakeup@126.com.
Wen
W
Zhang
College of Wildlife Resources, Northeast Forestry University, 26 Hexing Road, Harbin, Heilongjiang Province, 150040, China. xingmingweinefu@sina.com.
Mingwei
M
Xing
D000818:Animals; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000009:adverse effects* / Q000494:pharmacology; D030161:Avian Proteins / Q000096:biosynthesis*; D002645:Chickens / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D010087:Oxides / Q000009:adverse effects* / Q000494:pharmacology
D001152:Arsenicals; D030161:Avian Proteins; D006360:Heat-Shock Proteins; D010087:Oxides; D000077237:Arsenic Trioxide
As2O3; Cock; Heat shock proteins; Liver