Coverage percentage and raman measurement of cross-tile and scaffold cross-tile based DNA nanostructures.
We present two free-solution annealed DNA nanostructures consisting of either cross-tile CT1 or CT2. The proposed nanostructures exhibit two distinct structural morphologies, with one-dimensional (1D) nanotubes for CT1 and 2D nanolattices for CT2. When we perform mica-assisted growth annealing with CT1, a dramatic dimensional change occurs where the 1D nanotubes transform into 2D nanolattices due to the presence of the substrate. We assessed the coverage percentage of the 2D nanolattices grown on the mica substrate with CT1 and CT2 as a function of the concentration of the DNA monomer. Furthermore, we fabricated a scaffold cross-tile (SCT), which is a new design of a modified cross-tile that consists of four four-arm junctions with a square aspect ratio. For SCT, eight oligonucleotides are designed in such a way that adjacent strands with sticky ends can produce continuous arms in both the horizontal and vertical directions. The SCT was fabricated via free-solution annealing, and self-assembled SCT produces 2D nanolattices with periodic square cavities. All structures were observed via atomic force microscopy. Finally, we fabricated divalent nickel ion (Ni(2+))- and trivalent dysprosium ion (Dy(3+))-modified 2D nanolattices constructed with CT2 on a quartz substrate, and the ion coordinations were examined via Raman spectroscopy.
Sungkyunkwan Advanced Institute of Nanotechnology (SAINT) and Department of Physics, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Bramaramba
B
Gnapareddy
Korea Research Institute of Standards and Science, Daejeon 305-340, Republic of Korea.
Sang Jung
SJ
Ahn
Sungkyunkwan Advanced Institute of Nanotechnology (SAINT) and Department of Physics, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Sreekantha Reddy
SR
Dugasani
Sungkyunkwan Advanced Institute of Nanotechnology (SAINT) and School of Mechanical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Jang Ah
JA
Kim
Affiliated Colleges at Hafr Al-Batin, King Fahd University of Petroleum and Minerals, P.O. Box 1803, 31991 Hafr Al-Batin, Saudi Arabia and Interdisciplinary Research Center in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology, Lahore 54000, Pakistan.
Rashid
R
Amin
Sungkyunkwan Advanced Institute of Nanotechnology (SAINT) and Department of Physics, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Sekhar Babu
SB
Mitta
Sungkyunkwan Advanced Institute of Nanotechnology (SAINT) and Department of Physics, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Srivithya
S
Vellampatti
Sungkyunkwan Advanced Institute of Nanotechnology (SAINT) and Department of Physics, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Byeonghoon
B
Kim
Sungkyunkwan Advanced Institute of Nanotechnology (SAINT) and School of Mechanical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Atul
A
Kulkarni
Sungkyunkwan Advanced Institute of Nanotechnology (SAINT) and School of Mechanical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Taesung
T
Kim
Department of Bionanotechnology, Gachon University, Seongnam 461-701, Republic of Korea.
Kyusik
K
Yun
Department of Materials Science and Engineering, North Carolina State University, Raleigh, NC 27606, USA. Electronic address: thlabean@ncsu.edu.
Thomas H
TH
LaBean
Sungkyunkwan Advanced Institute of Nanotechnology (SAINT) and Department of Physics, Sungkyunkwan University, Suwon 440-746, Republic of Korea. Electronic address: sunghapark@skku.edu.
Sung Ha
SH
Park
D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures; D013059:Spectrum Analysis, Raman / Q000379:methods*
D004247:DNA
Coverage; DNA self-assembley; Ion doping; Raman; Substrate-assisted growth