A LC-MS/MS method for therapeutic drug monitoring of carbamazepine, lamotrigine and valproic acid in DBS.
BACKGROUND
Therapeutic drug monitoring of antiepileptic drugs in children with epilepsy assists for personalized drug therapy but require numerous patient visits for venous blood sampling. DBS is an alternative matrix applicable to home sampling which can save time and reduce stress for this patient group.


RESULTS
A fast LC-MS/MS method for quantification of carbamazepine, lamotrigine and valproic acid based on DBS sampling was developed. The method showed linearity in therapeutically relevant concentration ranges and compatible with unknown volume sampling and expected hematocrit range of the patient group.


CONCLUSIONS
A LC-MS/MS method for the three most commonly used antiepileptic drugs has been fully validated and clinically applied on DBSs from patients at the neuropediatric clinic at Karolinska University Hospital.
Department of Laboratory Medicine, Clinical Pharmacology, Karolinska Institute, Stockholm, Sweden.
Camilla
C
Linder
Chemistry and Drug Metabolism section, National Institute on Drug Abuse, National Institute of Health, Baltimore, MD, USA.
Maria
M
Andersson
Department of Clinical Science, Technology & Intervention, Division of Pediatrics, Karolinska University Hospital & Karolinska Institute, Stockholm, Sweden.
Katarina
K
Wide
Department of Laboratory Medicine, Clinical Pharmacology, Karolinska Institute, Stockholm, Sweden.
Olof
O
Beck
Department of Laboratory Medicine, Clinical Pharmacology, Karolinska Institute, Stockholm, Sweden.
Anton
A
Pohanka
D000927:Anticonvulsants / Q000097:blood*; D002138:Calibration; D002220:Carbamazepine / Q000097:blood; D002648:Child; D002853:Chromatography, Liquid / Q000379:methods*; D059788:Dried Blood Spot Testing / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods*; D004827:Epilepsy / Q000188:drug therapy; D006801:Humans; D000077213:Lamotrigine; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014227:Triazines / Q000097:blood; D014635:Valproic Acid / Q000097:blood
D000927:Anticonvulsants; D014227:Triazines; D002220:Carbamazepine; D014635:Valproic Acid; D000077213:Lamotrigine