Cryo-EM: Spinning the Micelles Away.
Structural characterization of integral membrane proteins (MPs) demands that the samples be pure, monodisperse, and stable. Detergents are required to extract MPs from the lipid bilayer in which they reside and to stabilize them for downstream biophysical analyses. Some of the best MP-stabilizing detergents pose problems for cryo-EM studies, but in this issue of Structure, Hauer et al. (2015) now offer a solution called GraDeR.
Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA. Electronic address: satinder.k.singh@yale.edu.
Satinder K
SK
Singh
Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA. Electronic address: fred.sigworth@yale.edu.
Fred J
FJ
Sigworth
D000818:Animals; D020285:Cryoelectron Microscopy / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification*
D008565:Membrane Proteins