A single nanoparticle-based sensor for hydrogen peroxide (H2O2) via cytochrome c-mediated plasmon resonance energy transfer.
Herein, we report a novel method for H2O2 detection based on a single plasmonic nanoprobe via cytochrome c (Cyt c)-mediated plasmon resonance energy transfer (PRET). Dynamic spectral changes were observed in the fingerprint quenching dip of a single plasmonic nanoprobe in response to changes in the redox state of Cyt c, induced by H2O2. Based on the changes in the spectral profile of the single plasmonic nanoprobe, H2O2 was successfully detected in a wide concentration range from 100 mM to 10 nM, including physiologically relevant micromolar and nanomolar concentrations.
Nanobiointerface Laboratory, Department of Life science, University of Seoul, Dongdaemun-ku, Seoul 130-743, Republic of Korea. inheechoi1@uos.ac.kr.
Yura
Y
Kim
Nanobiointerface Laboratory, Department of Life science, University of Seoul, Dongdaemun-ku, Seoul 130-743, Republic of Korea. inheechoi1@uos.ac.kr.
Ji Youn
JY
Park
Nanobiointerface Laboratory, Department of Life science, University of Seoul, Dongdaemun-ku, Seoul 130-743, Republic of Korea. inheechoi1@uos.ac.kr.
Hye Young
HY
Kim
Department of Chemical and Biological Engineering, Seoul National University, Gwanak-ku, Seoul 151-742, Republic of Korea.
Minzae
M
Lee
Department of Chemical and Biological Engineering, Seoul National University, Gwanak-ku, Seoul 151-742, Republic of Korea.
Jongheop
J
Yi
Nanobiointerface Laboratory, Department of Life science, University of Seoul, Dongdaemun-ku, Seoul 130-743, Republic of Korea. inheechoi1@uos.ac.kr.
Inhee
I
Choi
D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction
D006046:Gold; D045304:Cytochromes c; D006861:Hydrogen Peroxide