The adsorption of allergoids and 3-O-desacyl-4'-monophosphoryl lipid A (MPL®) to microcrystalline tyrosine (MCT) in formulations for use in allergy immunotherapy.
Infectious disease vaccine potency is affected by antigen adjuvant adsorption. WHO and EMA guidelines recommend limits and experimental monitoring of adsorption in vaccines and allergy immunotherapies. Adsorbed allergoids and MPL® in MATA-MPL allergy immunotherapy formulations effectively treat IgE mitigated allergy. Understanding vaccine antigen adjuvant adsorption allows optimisation of potency and should be seen as good practice; however current understanding is seldom applied to allergy immunotherapies. The allergoid and MPL® adsorption to MCT in MATA-MPL allergy immunotherapy formulations was experimental determination using specific allergen IgE allerginicity and MPL® content methods. Binding forces between MPL® and MCT were investigated by competition binding experiments. MATA-MPL samples with different allergoids gave results within 100-104% of the theoretical 50μg/mL MPL® content. Unmodified drug substance samples showed significant desirable IgE antigenicity, 1040-170 QAU/mL. MATA-MPL supernatant samples with different allergoids gave results of ≤2 μg/mL MPL® and ≤0.1-1.4 QAU/mL IgE antigenicity, demonstrating approximately ≥96 & 99% adsorption respectively. Allergoid and MPL® adsorption in different MATA-MPL allergy immunotherapy formulations is consistent and meets guideline recommendations. MCT formulations treated to disrupt electrostatic, hydrophobic and ligand exchange interactions, gave an MPL® content of ≤2 μg/mL in supernatant samples. MCT formulations treated to disrupt aromatic interactions, gave an MPL® content of 73-92 μg/mL in supernatant samples. MPL® adsorption to l-tyrosine in MCT formulations is based on interactions between the 2-deoxy-2-aminoglucose backbone on MPL® and aromatic ring of l-tyrosine in MCT, such as C-H⋯π interaction. MCT could be an alternative adjuvant depot for some infectious disease antigens.
Allergy Therapeutics, Plc, Dominion Way, Worthing BN14 8SA, United Kingdom.
A J
AJ
Bell
Allergy Therapeutics, Plc, Dominion Way, Worthing BN14 8SA, United Kingdom.
M D
MD
Heath
Allergy Therapeutics, Plc, Dominion Way, Worthing BN14 8SA, United Kingdom.
S J
SJ
Hewings
Allergy Therapeutics, Plc, Dominion Way, Worthing BN14 8SA, United Kingdom.
M A
MA
Skinner
D000276:Adjuvants, Immunologic / Q000737:chemistry; D000327:Adsorption; D000080384:Allergoids; D018926:Anti-Allergic Agents / Q000737:chemistry*; D008050:Lipid A / Q000031:analogs & derivatives* / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D014443:Tyrosine / Q000737:chemistry*
D000276:Adjuvants, Immunologic; D000080384:Allergoids; D018926:Anti-Allergic Agents; D008050:Lipid A; D010936:Plant Extracts; C586899:ragweed MATA MPL; D014443:Tyrosine
Adjuvant adsorption; Allergoid; C–H⋯π interactions; Microcrystalline tyrosine; Modified allergen tyrosine adsorbed; Monophosphoryl lipid A