Comparison of Different Irrigants in the Removal of Endotoxins and Cultivable Microorganisms from Infected Root Canals.
This study was conducted to compare the effectiveness of different irrigants used to remove endotoxins and cultivable microorganisms during endodontic therapy. Forty root canals were contaminated and divided into groups according to the irrigant: 2% NaOCl + surfactant, 2% CHX, 2.5% NaOCl, and pyrogen-free saline solution (control). Samples were collected after root canal contamination (S1), after instrumentation (S2), and 7 days after instrumentation (S3). Microorganisms and endotoxins were recovered from 100% of the contaminated root canals (S1). At S2, 2% NaOCl + surfactant, 2% CHX, and 2.5% NaOCl were able to completely eliminate cultivable microorganisms. At S3, both 2% CHX and 2.5% NaOCl were effective in preventing C. albicans and E. coli regrowth, but E. faecalis was still detected. No microorganism species was recovered from root canals instrumented with 2% NaOCl + surfactant. At S2, a higher percentage value of endotoxin reduction was found for 2% NaOCl + surfactant (99.3%) compared to 2% CHX (98.9%) and 2.5% NaOCl (97.18%) (p < 0.05). Moreover, at S3, 2% NaOCl + surfactant (100%) was the most effective irrigant against endotoxins. All irrigants tested were effective in reducing microorganisms and endotoxins from root canals. Moreover, 2% NaOCl + surfactant was the most effective irrigant against endotoxins and regrowth of microorganisms.
Department of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), São José dos Campos 12245-000, SP, Brazil.
Marcia Carneiro
MC
Valera
Department of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), São José dos Campos 12245-000, SP, Brazil.
Flávia Goulart da Rosa
FG
Cardoso
Department of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), São José dos Campos 12245-000, SP, Brazil.
Adriana
A
Chung
Department of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), São José dos Campos 12245-000, SP, Brazil.
Ana Cláudia Carvalho
AC
Xavier
Department of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), São José dos Campos 12245-000, SP, Brazil.
Mariana Diehl
MD
Figueiredo
Department of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), São José dos Campos 12245-000, SP, Brazil.
Frederico Canato
FC
Martinho
Department of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), São José dos Campos 12245-000, SP, Brazil.
Renato Miotto
RM
Palo
D058491:Bacterial Load; D002176:Candida albicans / Q000187:drug effects; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004731:Endotoxins; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D011671:Pulpitis / Q000188:drug therapy* / Q000382:microbiology*; D012388:Root Canal Irrigants / Q000008:administration & dosage*
D004731:Endotoxins; D012388:Root Canal Irrigants