Characterization and effects of miR-21 expression in esophageal cancer.
The aim of this study was to investigate the expression of miR-21 in esophageal cancer and the impact of miR-21 on apoptosis, invasion, and the expression of target genes in esophageal cancer cells. Fluorescence quantitative polymerase chain reaction analysis was used to detect the expression of miR-21 in human esophageal tissues, adjacent tissues, and an esophageal cancer cell line (TE-13). The antisense miR-21 oligonucleotide was generated commercially using the solid-phase chemical synthesis method. Transient transfection was used to transfect esophageal cancer cells (TE-13 antisense and TE-13 control cells). Flow cytometry and Transwell cell assays were used to detect the apoptosis and invasion of esophageal cancer cells, respectively. The western blot method was used to detect the expression of PTEN, PDCD4, and K-ras proteins. These analyses determined that mir-21 expression significantly increased in esophageal cancer tissues and in TE-13 cells, and that this phenomenon was not associated with staging or lymph node metastasis. The apoptosis rate of TE-13 control cells was lower than that of antisense TE-13 cells indicating an enhanced invasive ability. In tissues adjacent to esophageal cancer and in TE-13 antisense cells, the expression of PTEN and PDCD4 was found to be higher than that in the control group, whereas the expression of K-ras showed the opposite pattern. Together, these results suggest that miR- 21 might be involved in the development and metastasis of esophageal cancer, through interaction with its PDCD4 and K-ras target genes.
Department of Thoracic Surgery, The Fourth Hospital Affiliated with Hebei Medical University, Shijiazhuang, Hebei, China.
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Wen
Department of Thoracic Surgery, The Fourth Hospital Affiliated with Hebei Medical University, Shijiazhuang, Hebei, China.
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YF
Zhang
Department of Thoracic Surgery, The Fourth Hospital Affiliated with Hebei Medical University, Shijiazhuang, Hebei, China.
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Li
First Hospital of Xinji City in Hebei Province, Xinji, Hebei, China.
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Liu
Department of Thoracic Surgery, The Fourth Hospital Affiliated with Hebei Medical University, Shijiazhuang, Hebei, China.
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Lv
Department of Thoracic Surgery, The Fourth Hospital Affiliated with Hebei Medical University, Shijiazhuang, Hebei, China.
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Li
Department of Thoracic Surgery, The Fourth Hospital Affiliated with Hebei Medical University, Shijiazhuang, Hebei, China.
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YZ
Xu
Department of Thoracic Surgery, The Fourth Hospital Affiliated with Hebei Medical University, Shijiazhuang, Hebei, China.
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Zhu
Department of Thoracic Surgery, The Fourth Hospital Affiliated with Hebei Medical University, Shijiazhuang, Hebei, China.
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Tian
D000368:Aged; D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage / Q000235:genetics; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis; D016601:RNA-Binding Proteins / Q000096:biosynthesis; D014162:Transfection; D018631:ras Proteins / Q000096:biosynthesis
D051017:Apoptosis Regulatory Proteins; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; C114487:PDCD4 protein, human; D016601:RNA-Binding Proteins; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; D018631:ras Proteins