Selective carotenoid accumulation by varying nutrient media and salinity in Synechocystis sp. CCNM 2501.
Nutrients are the deciding factors in the biological production of bioactive compounds. Various growth media like BG11, Zarrouk's and Chu's 10 were studied for carotenoid production in Synechocystis sp. CCNM 2501. Maximum carotenoid content (dry weight basis) was found in Zarrouk's medium (ZM, 7.99mgg(-1)) followed by BG11 (5.13mgg(-1)). Echinenone content was 4 times higher in ZM (3.81mgg(-1)) as compared to BG11 (0.95mgg(-1)) and Chu's 10 (0.77mgg(-1)). Being an economical medium, BG11 was selected for carotenoid production. Further, increase in salinity from 0 to 0.2M in BG11 medium increases total carotenoid content from 5.82 to 7.05mgg(-1) and later it declines to 6.23mgg(-1) (1M). 3 times more β-carotene is produced at 1M salinity as compared to control BG11. The variation in carotenoid composition with change in nutrients/salinity can be a good strategy to enhance certain targeted carotenoids.
Division of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute Premises, Bhavnagar 364002, India.
Chetan
C
Paliwal
Division of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute Premises, Bhavnagar 364002, India.
Imran
I
Pancha
Division of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute Premises, Bhavnagar 364002, India.
Tonmoy
T
Ghosh
Division of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute Premises, Bhavnagar 364002, India.
Rahulkumar
R
Maurya
Division of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute Premises, Bhavnagar 364002, India.
Kaumeel
K
Chokshi
Division of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute Premises, Bhavnagar 364002, India.
S V
SV
Vamsi Bharadwaj
Division of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute Premises, Bhavnagar 364002, India.
Shristi
S
Ram
Division of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Central Salt and Marine Chemicals Research Institute Premises, Bhavnagar 364002, India. Electronic address: smishra@csmcri.org.
Sandhya
S
Mishra
D002338:Carotenoids / Q000032:analysis / Q000096:biosynthesis*; D003470:Culture Media; D054712:Salinity; D046939:Synechocystis / Q000737:chemistry / Q000378:metabolism*; D019207:beta Carotene / Q000032:analysis / Q000096:biosynthesis*
D003470:Culture Media; D019207:beta Carotene; D002338:Carotenoids; C105515:echinenone
Carotenoid; Cyanobacteria; Productivity; Salinity