Biochemical characterisation of the neuraminidase pool of the human gut symbiont Akkermansia muciniphila.
Since the isolation and identification of Akkermansia muciniphila one decade ago, much attention has been drawn to this gut bacterium due to its role in obesity and type 2 diabetes. This report describes the discovery and biochemical characterisation of all four putative neuraminidases annotated in the A. muciniphila genome. Recombinantly expressed candidate genes, which were designated Am0705, Am0707, Am1757 and Am2085, were shown to cover complementary pH ranges between 4.0 and 9.5. Temperature optima of the enzymes lay between 37 and 42 °C. All four enzymes were strongly inhibited by Cu(2+) and Zn(2+), and loss of activity after the addition of EDTA suggests that all neuraminidases, with the exception of Am0707, require divalent metal ions for their catalytic function. Chemoenzymatically synthesised α2,3- and α2,6-linked indoyl-sialosides were utilised to determine the regioselectivity and substrate promiscuity of the neuraminidases towards C5-modifications of sialic acids with N-acetyl-, N-glycolyl-, N-propionyl-, or hydroxyl-groups. The combination of simple purification procedures and good activities of some of the characterised neuraminidases makes them potentially interesting as tools in bioanalytical or industrial applications.
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.
Kun
K
Huang
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.
Mao M
MM
Wang
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.
Anna
A
Kulinich
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.
Hong L
HL
Yao
Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.
Hong Y
HY
Ma
Manchester Institute of Biotechnology and School of Chemistry, The University of Manchester, Manchester, United Kingdom.
Juana E R
JE
Martínez
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.
Xu C
XC
Duan
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.
Huan
H
Chen
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.
Zhi P
ZP
Cai
Manchester Institute of Biotechnology and School of Chemistry, The University of Manchester, Manchester, United Kingdom.
Sabine L
SL
Flitsch
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China. Electronic address: lichen.liu@njau.edu.cn.
Li
L
Liu
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China. Electronic address: josef.voglmeir@njau.edu.cn.
Josef
J
Voglmeir
D003300:Copper / Q000737:chemistry; D016680:Genome, Bacterial; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology*; D009439:Neuraminidase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature; D061327:Verrucomicrobia / Q000145:classification / Q000235:genetics* / Q000378:metabolism*
D003300:Copper; D009439:Neuraminidase
Akkermansia muciniphila; Indoyl-sialosides; Intestinal glycosidases; Neuraminidase; Sialidase