Promoters of AaGL2 and AaMIXTA-Like1 genes of Artemisia annua direct reporter gene expression in glandular and non-glandular trichomes.
Herein, we report cloning and analysis of promoters of GLABRA2 (AaGL2) homolog and a MIXTA-Like (AaMIXTA-Like1) gene from Artemisia annua. The upstream regulatory regions of AaGL2 and AaMIXTA-Like1 showed the presence of several crucial cis-acting elements. Arabidopsis and A. annua seedlings were transiently transfected with the promoter-GUS constructs using a robust agro-infiltration method. Both AaGL2 and AaMIXTA-Like1 promoters showed GUS expression preferentially in Arabidopsis single-celled trichomes and glandular as well as T-shaped trichomes of A. annua. Transgenic Arabidopsis harboring constructs in which AaGL2 or AaMIXTA-Like1 promoters would control GFP expression, showed fluorescence emanating specifically from trichome cells. Our study provides a fast and efficient method to study trichome-specific expression, and 2 promoters that have potential for targeted metabolic engineering in plants.
Sunita
S
Jindal
Bendangchuchang
B
Longchar
a Biotechnology Division; CSIR-Central Institute of Medicinal and Aromatic Plants ; Lucknow , India.
Alka
A
Singh
a Biotechnology Division; CSIR-Central Institute of Medicinal and Aromatic Plants ; Lucknow , India.
Vikrant
V
Gupta
D031172:Artemisia annua / Q000235:genetics*; D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D011401:Promoter Regions, Genetic; D064201:Trichomes / Q000235:genetics*
AaGL2 promoter; AaMIXTA-Like1 promoter; cis-elements; glandular trichome; non-glandular trichome