Protective effect of penehyclidine hydrochloride on lipopolysaccharide-induced acute kidney injury in rat.
We aimed to observe the effect of penehyclidine hydrochloride (PHC) on lipopolysaccharide (LPS)-induced acute kidney injury in rats and expression of tight junction proteins ZO-1 and occludin. Adult male Sprague-Dawley (SD) rats were divided randomly (N = 10) into control group (C), LPS group (LPS), low-dose PHC group (L-PHC), and high-dose PHC group (H-PHC). All rats, except C group, received a vena caudalis injection of 5.0 mg/kg LPS; after 30 min, rats in L-PHC and H-PHC groups received a vena caudalis injection of 0.3 and 0.9 mg/kg PHC. After 24 h, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, serum creatinine (Scr), and blood urea nitrogen (BUN) were detected. Histopathological changes and expression of ZO-1 and occludin were observed in renal tissues. Versus levels of TNF-α (38.5 ± 9.0), IL-1β (46.3 ± 12.7), Scr (37.2 ± 9.3), and BUN (6.5 ± 1.1) in control group, those in LPS group, TNF-α (159.0 ± 21.3), IL-1β (130.8 ± 18.7), Scr (98.5 ± 18.2), and BUN (12.8 ± 1.8), increased obviously (P < 0.05), with significantly structural changes and decreases of ZO-1 and occludin. However, TNF-α (111.3 ± 11.6), IL-1β (78.4 ± 14.3), Scr (51.3 ± 12.5), BUN (8.1 ± 1.2) in H-PHC group, and TNF-α (120.8 ± 14.3), IL-1β (92.5 ± 19.0), Scr (56.7 ± 14.7), BUN (9.7 ± 1.6) in L-PHC group were obviously decreased (P < 0.05). PHC has protective effects on acute kidney injury in sepsis, including abatement of renal tissue inflammation and functional improvement, potentially by upregulating ZO-1 and occludin.
Department of Anesthesiology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
H J
HJ
Cao
Department of Anesthesiology, The General Hospital of Shenyang Military Region, Shenyang, Liaoning Province, China.
D M
DM
Yu
Department of Anesthesiology, The General Hospital of Shenyang Military Region, Shenyang, Liaoning Province, China.
T Z
TZ
Zhang
Department of Anesthesiology, The General Hospital of Shenyang Military Region, Shenyang, Liaoning Province, China.
J
J
Zhou
Department of Anesthesiology, The General Hospital of Shenyang Military Region, Shenyang, Liaoning Province, China.
K Y
KY
Chen
Department of Gynecology and Obstetrics, The General Hospital of Shenyang Military Region, Shenyang, Liaoning Province, China.
J
J
Ge
Department of Anesthesiology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China lingpei49@vip.sina.com.
L
L
Pei
D058186:Acute Kidney Injury / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D007668:Kidney / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D008070:Lipopolysaccharides / Q000009:adverse effects*; D008297:Male; D062793:Occludin / Q000235:genetics / Q000378:metabolism; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D011812:Quinuclidines / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D062826:Zonula Occludens-1 Protein / Q000235:genetics / Q000378:metabolism
D016207:Cytokines; D008070:Lipopolysaccharides; D062793:Occludin; D020011:Protective Agents; D011812:Quinuclidines; D062826:Zonula Occludens-1 Protein; C486140:penehyclidine; D003404:Creatinine