Electrochemical aptasensor of cardiac troponin I for the early diagnosis of acute myocardial infarction.
Cardiac troponin I (cTnI) is well-known as a promising biomarker for the early diagnosis of acute myocardial infarction (AMI). In this work, single-stranded DNA aptamers against cTnI were identified by the Systematic Evolution of Ligands by Exponential enrichment (SELEX) method. The aptamer candidates exhibited a high selectivity and sensitivity toward both cTnI and the cardiac Troponin complex. The binding affinities of each aptamer were evaluated based on their dissociation constants (Kd) by surface plasma resonance. The Tro4 aptamer that had the highest binding capacity to cTnI showed a very low Kd value (270 pM) compared with that of a cTnI antibody (20.8 nM). Furthermore, we designed a new electrochemical aptasensor based on square wave voltammetry using ferrocene-modified silica nanoparticles. The developed aptasensor demonstrated an excellent analytical performance for cTnI with a wide linear range of 1-10 000 pM in a buffer and a detection limit of 1.0 pM (24 pg/mL; S/N = 3), which was noticeably lower than the cutoff values (70-400 pg/mL). The specificity of the aptamers was also examined using nontarget proteins, demonstrating that the proposed sensor responded to only cTnI. In addition, cTnI was successfully detected in a human serum albumin solution. On the basis of the calibration curve that was constructed, the concentrations of cTnI in a solution supplemented with human serum were effectively measured. The calculated values correlated well with the actual concentrations of cTnI. It is anticipated that the highly sensitive and selective aptasensor for cTnI could be readily applicable for the accurate diagnosis of AMI.
Department of Chemistry, Pohang University of Science and Technology , 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea.
Hunho
H
Jo
Department of Chemistry, Kwangwoon University , Wolgye-Dong, Nowon-Gu, Seoul 139-701, South Korea.
Hyunwoo
H
Gu
Department of Chemistry, Pohang University of Science and Technology , 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea.
Weejeong
W
Jeon
Department of Chemistry, Pohang University of Science and Technology , 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea.
Hyungjun
H
Youn
Department of Chemistry, Pohang University of Science and Technology , 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea.
Jin
J
Her
Department of Chemistry, Pohang University of Science and Technology , 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea.
Seong-Kyeong
SK
Kim
Department of Chemistry, Kwangwoon University , Wolgye-Dong, Nowon-Gu, Seoul 139-701, South Korea.
Jeongbong
J
Lee
Department of Chemistry, Kwangwoon University , Wolgye-Dong, Nowon-Gu, Seoul 139-701, South Korea.
Jae Ho
JH
Shin
Department of Chemistry, Pohang University of Science and Technology , 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea.
Changill
C
Ban
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods; D042241:Early Diagnosis; D055664:Electrochemical Techniques / Q000379:methods; D005296:Ferrous Compounds / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D057230:Limit of Detection; D000075163:Metallocenes; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis*; D053758:Nanoparticles / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D019210:Troponin I / Q000032:analysis / Q000097:blood*
D052157:Aptamers, Nucleotide; D005296:Ferrous Compounds; D000075163:Metallocenes; D019210:Troponin I; D012822:Silicon Dioxide; C004998:ferrocene