SV40 transcription terminators stabilize the activity of regulatory elements in Drosophila melanogaster.
To ensure stable operation of transgenic systems and maintain reporter gene expression at a certain level, it is necessary to search for the conditions that protect the activity of regulatory elements. In this study, we have shown that the SV40 transcription terminators flanking the transgene protect enhancers and silencers of Drosophila and ensure their efficient functioning.
Institute of Gene Biology, Russian Academy of Sciences, ul. Vavilova 34/5, Moscow, 119334, Russia, yermaxbio@yandex.ru.
D A
DA
Chetverina
P V
PV
Elizar'ev
P G
PG
Georgiev
M M
MM
Erokhin
D000818:Animals; D030801:Animals, Genetically Modified; D004331:Drosophila melanogaster / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D010641:Phenotype; D037941:Silencer Elements, Transcriptional / Q000235:genetics*; D013728:Terminator Regions, Genetic / Q000235:genetics*