Molecular Basis for Stellatic Acid Biosynthesis: A Genome Mining Approach for Discovery of Sesterterpene Synthases.
The search for a new sesterterpene synthase in the genome of Emericella variecolor, which reportedly produces diverse sesterterpenoids, is described. One gene product (a chimeric protein with prenyltransferase and terpene cyclase domains) led to the synthesis of a novel tricyclic sesterterpene, stellata-2,6,19-triene (1), from DMAPP and IPP, and the hydrocarbon was further transformed into stellatic acid (2) by cytochrome P450 monooxygenase encoded by the gene adjacent to the sesterterpene synthase gene.
Graduate School of Pharmaceutical Sciences, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Yudai
Y
Matsuda
Graduate School of Pharmaceutical Sciences, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Takaaki
T
Mitsuhashi
Graduate School of Pharmaceutical Sciences, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Zhiyang
Z
Quan
Graduate School of Pharmaceutical Sciences, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Ikuro
I
Abe
D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism*; D032842:Emericella / Q000201:enzymology / Q000235:genetics; D015394:Molecular Structure; D054830:Sesterterpenes / Q000737:chemistry* / Q000302:isolation & purification
D054830:Sesterterpenes; C000603891:stellata-2,6,19-triene; C000603890:stellatic acid; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase