3-D microarray and its microfabrication-free fluidic immunoassay device.
Conventional 2-D microarray is known to have high-throughput detection capability; however, the sensing spots density is significantly hindered by the spot-to-spot distance (gap) requirement for eliminating cross-talks between adjacent spots. Herein a new conceptual 3-D microarray device is proposed to significantly improve the spots density. To demonstrate advantages of the 3-D array, a microfabrication-free fluidic immunoassay device is further made by simply coupling an antibodies-arrayed glass cuboid into a circular glass tube. Rapid, sensitive and high-throughput flow-through immunoassays were accomplished with the 3-D array-based device for detection limits of 10-100 pg mL(-1) and wide dynamic range over 4-5 orders of magnitude in human serum with cancer biomarkers α-fetoprotein (AFP) and carcinoembryonic antigen (CEA) as model targets, holding great promise for practical clinical applications. The 3-D microarray device not only significantly increases the density of sensing spots, but also greatly enhances the mass transport for rapid immunoassay when using in a flow-through device.
Institute for Clean Energy & Advanced Materials, Faculty of Materials & Energy, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Southwest University, Chongqing 400715, China. Electronic address: ysliu@swu.edu.cn.
Yingshuai
Y
Liu
Institute for Clean Energy & Advanced Materials, Faculty of Materials & Energy, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Southwest University, Chongqing 400715, China.
Yuanyuan
Y
Zhang
Institute for Clean Energy & Advanced Materials, Faculty of Materials & Energy, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Southwest University, Chongqing 400715, China.
Zhisong
Z
Lu
Institute for Clean Energy & Advanced Materials, Faculty of Materials & Energy, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Southwest University, Chongqing 400715, China. Electronic address: ecmli@swu.edu.cn.
Chang Ming
CM
Li
D000906:Antibodies / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood; D002272:Carcinoembryonic Antigen / Q000097:blood; D005898:Glass / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D018625:Microscopy, Atomic Force; D011108:Polymers / Q000737:chemistry; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D059629:Signal-To-Noise Ratio; D000509:alpha-Fetoproteins / Q000032:analysis*
D000906:Antibodies; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D011108:Polymers; D000509:alpha-Fetoproteins
3-Dimensional microarray; Cancer biomarkers; Flow-through immunoassay; High-throughput; Microfabrication-free