Mussel adhesion-employed water-immiscible fluid bioadhesive for urinary fistula sealing.
Urinary fistulas, abnormal openings of a urinary tract organ, are serious complications and conventional management strategies are not satisfactory. For more effective and non-invasive fistula repair, fluid tissue adhesives or sealants have been suggested. However, conventional products do not provide a suitable solution due to safety problems and poor underwater adhesion under physiological conditions. Herein, we proposed a unique water-immiscible mussel protein-based bioadhesive (WIMBA) exhibiting strong underwater adhesion which was employed by two adhesion strategies of marine organisms; 3,4-dihydroxy-l-phenylalanine (DOPA)-mediated strong adhesion and water-immiscible coacervation. The developed biocompatible WIMBA successfully sealed ex vivo urinary fistulas and provided good durability and high compliance. Thus, WIMBA could be used as a promising sealant for urinary fistula management with further expansion to diverse internal body applications.
Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
Hyo Jeong
HJ
Kim
Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
Byeong Hee
BH
Hwang
Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
Seonghye
S
Lim
Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
Bong-Hyuk
BH
Choi
Department of Urology, Korea University School of Medicine, Seoul 136-705, Republic of Korea.
Seok Ho
SH
Kang
Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea. Electronic address: hjcha@postech.ac.kr.
Hyung Joon
HJ
Cha
D000818:Animals; D049872:Bivalvia; D004295:Dihydroxyphenylalanine / Q000494:pharmacology; D008297:Male; D011312:Pressure; D011506:Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D000267:Tissue Adhesions / Q000473:pathology; D014548:Urinary Fistula / Q000188:drug therapy*; D014867:Water / Q000737:chemistry*
D011506:Proteins; D011994:Recombinant Proteins; C037403:adhesive protein, mussel; D014867:Water; D004295:Dihydroxyphenylalanine
Complex coacervation; DOPA; Mussel adhesive proteins; Underwater adhesion; Urinary fistula