Twenty-eight days of exposure to 3454 m increases mitochondrial volume density in human skeletal muscle.
The role of hypoxia on skeletal muscle mitochondria is controversial. Studies superimposing exercise training on hypoxic exposure demonstrate an increase in skeletal muscle mitochondrial volume density (Mito(VD)) over equivalent normoxic training. In contrast, reductions in both skeletal muscle mass and Mito(VD) have been reported following mountaineering expeditions. These observations may, however, be confounded by negative energy balance, which may obscure the results. Accordingly we sought to examine the effects of high altitude hypoxic exposure on mitochondrial characteristics, with emphasis on Mito(VD), while minimizing changes in energy balance. For this purpose, skeletal muscle biopsies were obtained from nine lowlanders at sea level (Pre) and following 7 and 28 days of exposure to 3454 m. Maximal ergometer power output, whole body weight and composition, leg lean mass and skeletal muscle fibre area all remained unchanged following the altitude exposure. Transmission electron microscopy determined that intermyofibrillar (IMF) Mito(VD) was augmented (P = 0.028) by 11.5 ± 9.2% from Pre (5.05 ± 0.9%) to 28 Days (5.61 ± 0.04%). In contrast, there was no change in subsarcolemmal (SS) Mito(VD). As a result, total Mito(VD) (IMF + SS) was increased (P = 0.031) from 6.20 ± 1.5% at Pre to 6.62 ± 1.4% at 28 Days (7.8 ± 9.3%). At the same time no changes in mass-specific respiratory capacities, mitochondrial protein or antioxidant content were found. This study demonstrates that skeletal muscle Mito(VD) may increase with 28 days acclimation to 3454 m.
Zürich Centre for Integrative Human Physiology, Institute of Physiology, University of Zürich, Switzerland.|Health and Physical Education, School of Teaching and Learning, Western Carolina University, Cullowhee, NC, USA.|Physical Therapy Department, Western Carolina University, Cullowhee, NC, USA.
Robert A
RA
Jacobs
Zürich Centre for Integrative Human Physiology, Institute of Physiology, University of Zürich, Switzerland.
Anne-Kristine Meinild
AK
Lundby
Zürich Centre for Integrative Human Physiology, Institute of Physiology, University of Zürich, Switzerland.
Simone
S
Fenk
Zürich Centre for Integrative Human Physiology, Institute of Physiology, University of Zürich, Switzerland.
Saskia
S
Gehrig
Zürich Centre for Integrative Human Physiology, Institute of Physiology, University of Zürich, Switzerland.|Department of Environmental Physiology, School of Technology and Health, Royal Institute of Technology, Solna, Sweden.
Christoph
C
Siebenmann
Zürich Centre for Integrative Human Physiology, Institute of Physiology, University of Zürich, Switzerland.
Daniela
D
Flück
Zürich Centre for Integrative Human Physiology, Institute of Physiology, University of Zürich, Switzerland.
Niels
N
Kirk
Intensive Care Unit, University Hospital of Zurich, Switzerland.
Matthias P
MP
Hilty
Zürich Centre for Integrative Human Physiology, Institute of Physiology, University of Zürich, Switzerland.
Carsten
C
Lundby
D000064:Acclimatization; D000328:Adult; D000531:Altitude; D004734:Energy Metabolism; D005260:Female; D006801:Humans; D008297:Male; D008931:Mitochondria, Muscle / Q000378:metabolism* / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000166:cytology / Q000502:physiology*