Inhibitory effects of spironolactone on myocardial fibrosis in spontaneously hypertensive rats.
This study evaluated the inhibitory effects of spironolac-tone, a non-selective aldosterone receptor antagonist, on hypertension-induced myocardial fibrosis. Collagen I and III contents was detected in the myocardial tissue of spontaneously hypertensive rats (SHRs) after spironolactone administration. Twenty male SHRs were assigned to the spironolactone group or control group (N = 10 each); 7 Wistar-Kyoto rats (WKY) were also used. Spironolactone dissolved in ddH2O was administered via gavage at a dosage of 20 mg·kg(-1)·day(-1). Meanwhile, the control and WKY groups were administered equivalent volumes of ddH2O for 16 weeks. Western blotting was used to detect the contents of collagen I in myocardial tissue; observations were performed using polarizing microscopy, and the area integration and ratio of collagen I/III were subsequently calculated. Compared to the WKY group, col-lagen I synthesis was significantly higher in the control group (1.87 ± 0.2 vs 1.21 ± 0.7, P < 0.05). After 16 weeks of treatment, collagen I contents were significantly lower in the spironolactone group than in the control group (1.42 ± 0.05 vs 1.87 ± 0.2, P < 0.05). The ar-eas of collagen I and collagen I/III ratio were significantly smaller in the spironolactone group than in the control group (6400 ± 259 vs 12,019 ± 734 pixels, 15.64 ± 1.34 vs 20.8 ± 3.04 pixels, respec-tively; P < 0.05). However, there were no significant differences in the area of collagen III among the three groups. In conclusion, spi-ronolactone improves myocardial collagen deposition, preventing myocardial fibrosis in SHRs.
Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease, Department of Cardiology, Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin, China.
H
H
Zhao
Department of Cardiology, Affiliated Hospital of North China University of Science and Technology, Tangshan, China.
D W
DW
Gu
Department of Cardiology, Affiliated Hospital of North China University of Science and Technology, Tangshan, China.
H T
HT
Li
Department of Cardiology, Affiliated Hospital of North China University of Science and Technology, Tangshan, China.
Q F
QF
Ge
Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease, Department of Cardiology, Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin, China.
G P
GP
Li
D000818:Animals; D015153:Blotting, Western; D002452:Cell Count; D003094:Collagen / Q000378:metabolism; D005355:Fibrosis; D008297:Male; D008859:Microscopy, Polarization; D009206:Myocardium / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013148:Spironolactone / Q000494:pharmacology*
D013148:Spironolactone; D003094:Collagen