Physicochemical characteristics and biological activities of polysaccharide fractions from Phellinus baumii cultured with different methods.
Nine polysaccharide fractions were obtained from the fruiting bodies, submerged mycelia, and solid state fermented products of Phellinus baumii using different concentrations of ethanol precipitation. The chemical characteristics and in vitro immunological activities of the nine polysaccharide fractions were compared and studied. Results indicated that the fractions precipitated with 50% ethanol had higher yields of polysaccharides and submerged mycelia contributed to high extraction yields of polysaccharides and possessed higher polysaccharide contents. HPSEC-MALLS-RI analysis showed that the molecular weight (Mw) of polysaccharide fractions from these three materials decreased with the increasing of precipitated ethanol concentration. The Mw of fruiting body polysaccharide fractions ranged from 1.98×10(4)Da to 1.89×10(6)Da. Large-molecular-weight polysaccharides (from 2.11×10(6)Da to 2.01×10(7)Da) were found in submerged mycelia. Some lower-molecular-weight polysaccharide components were found in solid fermented products. Different culture methods contributed to significant differences in monosaccharide components and molar ratios. The 50% ethanol precipitated fractions exhibited more complexity on monosaccharide compositions comparing with fractions precipitated with 30% and 70% ethanol. Polysaccharide fractions derived from submerged mycelia exhibited higher macrophages stimulation activities. Submerged culture was found to be a suitable method to prepare active polysaccharides because of its short culture span and reasonable cost.
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, PR China; National Engineering Research Center of Edible Fungi, Shanghai, PR China; Shanghai University of Medicine & Health Sciences, Shanghai, PR China.
Tingting
T
Li
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, PR China; National Engineering Research Center of Edible Fungi, Shanghai, PR China; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, PR China. Electronic address: yangyan@saas.sh.cn.
Yan
Y
Yang
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, PR China; National Engineering Research Center of Edible Fungi, Shanghai, PR China; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, PR China. Electronic address: aliu-1980@163.com.
Yanfang
Y
Liu
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, PR China; National Engineering Research Center of Edible Fungi, Shanghai, PR China; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, PR China.
Shuai
S
Zhou
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, PR China; National Engineering Research Center of Edible Fungi, Shanghai, PR China; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, PR China.
Meng Qiu
MQ
Yan
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, PR China; National Engineering Research Center of Edible Fungi, Shanghai, PR China; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, PR China.
Di
D
Wu
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, PR China; National Engineering Research Center of Edible Fungi, Shanghai, PR China; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, PR China.
Jingsong
J
Zhang
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, PR China; National Engineering Research Center of Edible Fungi, Shanghai, PR China; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, PR China.
Chuanhong
C
Tang
D000818:Animals; D001487:Basidiomycota / Q000187:drug effects / Q000254:growth & development*; D018929:Cell Culture Techniques / Q000379:methods*; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D009569:Nitric Oxide / Q000096:biosynthesis; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D000067996:RAW 264.7 Cells
D009005:Monosaccharides; D011134:Polysaccharides; D009569:Nitric Oxide
Chemical characteristics; Culture methods; Immunological activities; Molecular weight; Polysaccharides