Sero-diagnosis of Echinococcus granulosus infection in dogs using faecal supernatant antigen.
In the present study, sero-diagnosis of Echinococcus granulosus infection in dogs was studied using 250 field serum samples, collected from stray dogs. The three serological techniques viz., latex agglutination test (LAT), Dot-ELISA and enzyme immunotransfer blot (EITB) were used to detect E. granulosus specific anti-bodies using fecal supernatant antigen. The LAT detected positivity in 53 (21.2 %) serum samples with the sensitivity and specificity of 100 and 78.80 % respectively. Whereas, the Dot-ELISA, showed positivity in 45 (18.0 %) samples and the sensitivity and specificity were 100 and 82.0 % respectively. The specific antibodies were detected in 47 (18.80 %) serum samples by EITB and the polypeptides of 45 and 34 kDa were identified in all the positive serum samples. The sensitivity and specificity of EITB were found to be 100 and 86.80 %, respectively.
Department of Veterinary Parasitology, Veterinary College, Shimoga, 577204 India.
K J
KJ
Ananda
Department of Veterinary Parasitology, Veterinary College, Hebbal, Bangalore, 560024 India.
Placid E
PE
D'Souza
Molecular Virology Lab, IVRI, Hebbal, Bangalore, 560024 India.
V V S
VV
Suryanarayana
Dog; Dot-ELISA; E. granulosus; EITB; LAT; Serodiagnosis