Chemoenzymatic Synthesis of Acyl Coenzyme A Substrates Enables in Situ Labeling of Small Molecules and Proteins.
A chemoenzymatic approach to generate fully functional acyl coenzyme A molecules that are then used as substrates to drive in situ acyl transfer reactions is described. Mass spectrometry based assays to verify the identity of acyl coenzyme A enzymatic products are also illustrated. The approach is responsive to a diverse array of carboxylic acids that can be elaborated to their corresponding coenzyme A thioesters, with potential applications in wide-ranging chemical biology studies that utilize acyl coenzyme A substrates.
Vinayak
V
Agarwal
Stefan
S
Diethelm
Lauren
L
Ray
Neha
N
Garg
Graduate School of Pharmaceutical Sciences, The University of Tokyo , Tokyo 113-0033, Japan.
Takayoshi
T
Awakawa
Pieter C
PC
Dorrestein
Bradley S
BS
Moore
D000214:Acyl Coenzyme A / Q000378:metabolism*; D002264:Carboxylic Acids / Q000737:chemistry; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry*
D000214:Acyl Coenzyme A; D002264:Carboxylic Acids; D011506:Proteins