Elimination of the artefact peaks in capillary electrophoresis determination of glutamate by using organic solvents in sample preparation.
Focusing on the demand from the food industry for fast and reliable alternative methods to control the quality of food products, we present in this paper a method for amino acid separation and glutamic acid quantification in complex matrices employing capillary electrophoresis with capacitively coupled contactless conductivity detection. We demonstrate by simulation and experimentally the use of organic solvents in sample preparation to prevent peak splitting and increase stacking in capillary electrophoretic separations of amino acids. Additionally, we obtained results for glutamic acid quantification comparable to those obtained via traditional methods used at industrial sites. We tested premium and low-cost samples with large variations in their glutamic acid content, which demonstrated the wide range of applicability of the method presented herein. The results of the proposed capacitively coupled contactless conductivity detection based capillary electrophoresis method agreed with those obtained by an enzymatic detector and ultra high performance liquid chromatography coupled to tandem mass spectrometry, considering a confidence level of 95%.
Chemistry Institute, State University of Campinas, Campinas, SP, Brazil.
Camila Dalben Madeira
CD
Campos
Faculty of Food Engineering, State University of Campinas, Campinas, SP, Brazil.
Patricia Aparecida
PA
de Campos Braga
Faculty of Food Engineering, State University of Campinas, Campinas, SP, Brazil.
Felix Guillermo Reyes
FG
Reyes
Chemistry Institute, State University of Campinas, Campinas, SP, Brazil.|Instituto Nacional de Ciência e Tecnologia em Bioanalítica (INCTBio), Campinas, SP, Brazil.
José Alberto Fracassi
JA
da Silva
D016477:Artifacts; D019075:Electrophoresis, Capillary / Q000379:methods*; D018698:Glutamic Acid / Q000032:analysis*; D012997:Solvents / Q000737:chemistry*; D045730:Soy Foods / Q000032:analysis
D012997:Solvents; D018698:Glutamic Acid
Amino acids; Capillary electrophoresis; Contactless conductivity detection; Peak splitting