Enterobacter tabaci sp. nov., a novel member of the genus Enterobacter isolated from a tobacco stem.
A Gram-stain negative, motile, rod-shaped bacterium, designated strain YIM Hb-3(T), was isolated from the stem of a tobacco plant. The strain was observed to form convex, circular and yellow-colored colonies. The predominant respiratory quinone was identified as Q-8. The major fatty acids (>5%) detected were C(16:1)ω7c and/or C(16:1)ω6c (summed feature 3), C(16:0), C(17:0)cyclo, C(18:1)ω7c and/or C(18:1)ω6c (summed feature 8), C(14:0)3-OH and/or iso-C(16:1)I (summed feature 2), C(14:0) and C(12:0). The genomic DNA G+C content was determined to be 54.8 mol%. Phylogenetic trees based on 16S rRNA gene sequences and multilocus sequence analysis showed that strain YIM Hb-3(T) had the closest phylogenetic relationship with Enterobacter mori LMG 25706(T). DNA-DNA relatedness value between strain YIM Hb-3(T) and E. mori LMG 25706(T) was 46.9 ± 3.8%. On the basis of phenotypic and chemotaxonomic data, phylogenetic analysis, and DNA-DNA relatedness value, strain YIM Hb-3(T) is considered to represent a novel species of the genus Enterobacter, for which the name Enterobacter tabaci sp. nov. is proposed. The type strain is YIM Hb-3(T) (=KACC 17832(T) =KCTC 42694(T)).
China Tobacco Yunnan Industrial Co. Ltd, Kunming, 650231, People's Republic of China.
Yan-Qing
YQ
Duan
China Tobacco Yunnan Industrial Co. Ltd, Kunming, 650231, People's Republic of China.|Life Science College, Southwest Forestry University, Kunming, 650224, People's Republic of China.
Xing-Kui
XK
Zhou
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.|Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu, 611130, People's Republic of China.
Li
L
Di-Yan
Kunming Xianghao Technology Co. Ltd., Kunming, 650204, People's Republic of China. doublelqq@163.com.
Qing-Qing
QQ
Li
China Tobacco Yunnan Industrial Co. Ltd, Kunming, 650231, People's Republic of China.
Li-Zhi
LZ
Dang
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.|Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, People's Republic of China.
Yong-Guang
YG
Zhang
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Li-Hong
LH
Qiu
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Salam
S
Nimaichand
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China. liwenjun3@mail.sysu.edu.cn.|Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, People's Republic of China. liwenjun3@mail.sysu.edu.cn.
Wen-Jun
WJ
Li
D004754:Enterobacter / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D010802:Phylogeny; D018547:Plant Stems / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014026:Nicotiana / Q000382:microbiology*
D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
16S rRNA gene; Enterobacter tabaci sp. nov.; MLSA; Nicotiana tabacum L.; Polyphasic taxonomy