Intoxication of a Young Girl Reveals the Pitfalls of GHB Rapid Screening.
The authors discuss the case of a 14-year-old girl who was transferred to the ICU of our hospital with ethanol intoxication (3.3 g/L), loss of consciousness (E5M3V1), and severe amnesia on recovery that was suspected of gamma-hydroxybutyric acid (GHB) intoxication. STAT toxicology screening may be necessary, when sexual assault under GHB intoxication is suspected. Therefore, the initial analysis of a urine sample was performed with a new enzymatic assay analysis for GHB. The enzymatic assay reported a GHB concentration of 26 mg/L, which is above the cut-off value of 10 mg/L. This cut-off value is to differentiate endogenous and exogenous levels because low levels of GHB occur naturally in the body. However, confirmation of these results by gas chromatography, which is common practice to confirm a positive GHB, gave a negative result. This discrepancy is probably contributed to interference of ethanol with the assay. This is a substantial downside of the GHB rapid screening, since the combination of GHB and ethanol is common. It is therefore advised to confirm that the positive GHB results are lower than 50 mg/L by gas chromatography, when using the rapid screening. This way the false-positive results and consequent inappropriate social and legal actions may be avoided.
*Department of Hospital Pharmacy, Erasmus MC, Rotterdam; and †Department of Pediatric Surgery and Intensive Care, Erasmus MC Sophia Children's Hospital, Rotterdam, the Netherlands.
Linda G
LG
Franken
Louise M
LM
Andrews
Valerie D
VD
Slooff
Saskia N
SN
de Wildt
Birgit C P
BC
Koch
D000293:Adolescent; D000435:Alcoholic Intoxication / Q000175:diagnosis*; D002849:Chromatography, Gas / Q000379:methods; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D006885:Hydroxybutyrates / Q000506:poisoning* / Q000652:urine; D008403:Mass Screening / Q000379:methods*; D015813:Substance Abuse Detection / Q000379:methods*
D006885:Hydroxybutyrates; C111420:4-hydroxybutyric acid