Distribution of Ribonucleoprotein and Protein-Only RNase P in Eukarya.
RNase P is the endonuclease that removes 5' leader sequences from tRNA precursors. In Eukarya, separate RNase P activities exist in the nucleus and mitochondria/plastids. Although all RNase P enzymes catalyze the same reaction, the different architectures found in Eukarya range from ribonucleoprotein (RNP) enzymes with a catalytic RNA and up to 10 protein subunits to single-subunit protein-only RNase P (PRORP) enzymes. Here, analysis of the phylogenetic distribution of RNP and PRORP enzymes in Eukarya revealed 1) a wealth of novel P RNAs in previously unexplored phylogenetic branches and 2) that PRORP enzymes are more widespread than previously appreciated, found in four of the five eukaryal supergroups, in the nuclei and/or organelles. Intriguingly, the occurrence of RNP RNase P and PRORP seems mutually exclusive in genetic compartments of modern Eukarya. Our comparative analysis provides a global picture of the evolution and diversification of RNase P throughout Eukarya.
Institut für Pharmazeutische Chemie, Philipps-Universität Marburg, Marburg, Germany.
Marcus
M
Lechner
Zentrum für Anatomie & Zellbiologie, Medizinische Universität Wien, Wien, Austria.
Walter
W
Rossmanith
Institut für Pharmazeutische Chemie, Philipps-Universität Marburg, Marburg, Germany.
Roland K
RK
Hartmann
Institut für Pharmazeutische Chemie, Philipps-Universität Marburg, Marburg, Germany.
Clemens
C
Thölken
Institut de Biologie Moléculaire des Plantes du CNRS, Strasbourg, France.
Bernard
B
Gutmann
Institut de Biologie Moléculaire des Plantes du CNRS, Strasbourg, France anthony.gobert@ibmp-cnrs.unistra.fr giege@unistra.fr.
Philippe
P
Giegé
Institut de Biologie Moléculaire des Plantes du CNRS, Strasbourg, France anthony.gobert@ibmp-cnrs.unistra.fr giege@unistra.fr.
Anthony
A
Gobert
D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D056890:Eukaryota / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017433:Protein Structure, Secondary; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism; D043262:Ribonuclease P / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
D012322:RNA Precursors; D016337:RNA, Catalytic; D012261:Ribonucleoproteins; D012313:RNA; D012343:RNA, Transfer; D043262:Ribonuclease P
PRORP; RNase P; eukaryal evolution; ribonucleoproteins; tRNA biogenesis