Parameter identification of a dynamic model of CHO cell cultures: an experimental case study.
In this paper, we address the problem of parameter identification in dynamic models of animal cultures, and we propose a step-by-step procedure, which gradually considers more detailed models. This procedure allows subsets of parameters to be estimated at each step, which can be used in the initialization of the next identification step. Finally, the full parameter set can be re-estimated starting from the results of the last step. The efficiency of the procedure is illustrated with a simulation case study and with the identification of a dynamic model from experimental data collected in CHO cell culture.
Automatic Control Department, University of Mons, 31 Boulevard Dolez, 7000, Mons, Belgium. ines.saraiva@umons.ac.be.
Ines
http://orcid.org/0000-0001-6429-7526
I
Saraiva
Automatic Control Department, University of Mons, 31 Boulevard Dolez, 7000, Mons, Belgium.
Alain
A
Vande Wouwer
Chemical and Biochemical Process Engineering Department, University of Mons, 56 Rue de l'Épargne, 7000, Mons, Belgium.
Anne-Lise
AL
Hantson
D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques; D006224:Cricetinae; D003412:Cricetulus; D008954:Models, Biological
Animal cell cultures; Mathematical modeling; Parameter estimation; Sensitivity analysis