Viral dominance of reassortants between canine influenza H3N2 and pandemic (2009) H1N1 viruses from a naturally co-infected dog.
BACKGROUND
Since avian-origin H3N2 canine influenza virus (CIV) was first identified in South Korea in 2008, the novel influenza virus has been reported in several countries in Asia. Reverse zoonotic transmission of pandemic H1N1 (2009) influenza virus (pH1N1) has been observed in a broad range of animal species. Viral dominance and characterization of the reassortants of both viruses was undertaken in the present study.


RESULTS
Here we describe the viral dominance of 23 CIV reassortants between pH1N1 and canine H3N2 influenza viruses from a naturally co-infected dog. These results indicate that the M gene of pandemic H1N1 and the HA gene of canine H3N2 are predominant in the reassortants. Furthermore, unlike the original canine H3N2 virus, some reassortants showed high pathogenicity in mice.


CONCLUSIONS
This study suggests that continuous monitoring of influenza infection in companion animals may be necessary to investigate the potential of the emergence of novel influenza viruses.
Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea. wsungna@gmail.com.
Woonsung
W
Na
Korea Zoonosis Research Institute, Chonbuk National University, Iksan, Republic of Korea. lks1314@jbnu.ac.kr.
Kwang-Soo
KS
Lyoo
Department of Pharmacy, College of Pharmacy, Korea University, Sejong, Republic of Korea. ssongdang@gmail.com.
Eun-jung
EJ
Song
Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea. mkhong@kribb.re.kr.
Minki
M
Hong
Department of Pharmacy, College of Pharmacy, Korea University, Sejong, Republic of Korea. paransearo@naver.com.
Minjoo
M
Yeom
Green Cross Veterinary Products, Yong-In, Republic of Korea. mhj1219@naver.com.
Hyoungjoon
H
Moon
Green Cross Veterinary Products, Yong-In, Republic of Korea. suyun@gcvp.co.kr.
Bo-Kyu
BK
Kang
Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea. golddj75@gmail.com.
Doo-Jin
DJ
Kim
Department of Pharmacy, College of Pharmacy, Korea University, Sejong, Republic of Korea. jkfrancis@korea.ac.kr.
Jeong-Ki
JK
Kim
Department of Pharmacy, College of Pharmacy, Korea University, Sejong, Republic of Korea. sds1@korea.ac.kr.
Daesub
D
Song
D000818:Animals; D060085:Coinfection / Q000662:veterinary* / Q000821:virology; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification*; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics / Q000302:isolation & purification*; D008810:Mice, Inbred C57BL; D009976:Orthomyxoviridae Infections / Q000662:veterinary* / Q000821:virology; D016865:Reassortant Viruses / Q000235:genetics / Q000302:isolation & purification*; D056910:Republic of Korea; D014763:Viral Matrix Proteins / Q000235:genetics; D014774:Virulence
D019267:Hemagglutinin Glycoproteins, Influenza Virus; C488936:M1 protein, Influenza A virus; D014763:Viral Matrix Proteins