Candida albicans bloodstream isolates in a German university hospital are genetically heterogenous and susceptible to commonly used antifungals.
From an eight-year-span, 99 Candida bloodstream isolates were collected at the University Hospital Wuerzburg, Germany. In this study, all strains were analyzed using molecular and phenotypic typing methods. Confirmatory species identification revealed three isolates that were initially diagnosed as C. albicans to be actually C. dubliniensis. Two isolates contained a mixed culture of C. albicans and C. glabrata, in one of the specimens both species could be separated while it was not possible to recover C. albicans in the other sample. The remaining 95 C. albicans isolates were profiled by multilocus sequence typing (MLST). Phylogenetic analyses showed a highly heterogenous collection of strains, associated with many different clades and constituting a set of new diploid sequence types (DST). For all strains with identical DST, patient data were reviewed for potential nosocomial transmission. In addition, all isolates were tested for their susceptibility to amphotericin B, caspofungin, fluconazole, itraconazole, posaconazole and voriconazole. No clinically relevant resistance could be detected. Furthermore, these data underline that correlation between minimal inhibitory concentrations for caspofungin and anidulafungin is low.
National Reference Center for Invasive Mycoses, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany; Septomics Research Center, Friedrich Schiller University and Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.
Johanna
J
Huyke
Septomics Research Center, Friedrich Schiller University and Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.
Ronny
R
Martin
National Reference Center for Invasive Mycoses, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.
Grit
G
Walther
Septomics Research Center, Friedrich Schiller University and Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.
Michael
M
Weber
National Reference Center for Invasive Mycoses, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.
Kerstin
K
Kaerger
Institut Pasteur, Unité Biologie et Pathogénicité Fongiques INRA USC2019 Laboratoire de Parasitologie-Mycologie, Service de Microbiologie, Hôpital Necker-Enfants Malades, Université Paris Descartes, Faculté de Médecine, Paris, France.
Marie-Elisabeth
ME
Bougnoux
University of Wuerzburg, Institute of Hygiene and Microbiology, Wuerzburg, Germany.
Johannes
J
Elias
National Reference Center for Invasive Mycoses, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany; Septomics Research Center, Friedrich Schiller University and Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany. Electronic address: oliver.kurzai@hki-jena.de.
Oliver
O
Kurzai
D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D058387:Candidemia / Q000382:microbiology*; D016000:Cluster Analysis; D025141:Drug Resistance, Fungal; D014644:Genetic Variation; D005858:Germany; D006785:Hospitals, University; D006801:Humans; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D016533:Mycological Typing Techniques; D010802:Phylogeny
D000935:Antifungal Agents
Anidulafungin; Bloodstream infection; Candida albicans; Fluconazole; Multi-locus sequence typing MLST; Resistance