Comparison of covalent and physical immobilization of lipase in gigaporous polymeric microspheres.
Lipase (EC 3.1.1.3) is a versatile enzyme which has been widely used in ester-reaction industries. We have previously discovered that gigaporous polystyrene (PST) microspheres can be used as a novel immobilization carrier for lipase. In this work, a series of gigaporous microspheres with different densities of epoxy group including poly(glycidyl methacrylate) (PGMA) and poly(styrene-co-glycidyl methacrylate) [P(ST-GMA)] were evaluated as lipase immobilization carriers, which were also compared with gigaporous PST microspheres and the commercial immobilized lipase Novozym 435. Lipase immobilized in gigaporous PGMA microspheres showed the highest activity yield, reusability, and stability as well as the best affinity for the substrate. The characterizations of adsorption curves, the change of epoxy group amounts, and hydrophobic-hydrophilic properties of the microspheres were carried out to investigate the interaction between lipase molecules and carriers. It was found that covalent binding played a key role in improving the properties of lipase immobilized in gigaporous PGMA microspheres.
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
Weichen
W
Wang
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
Weiqing
W
Zhou
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
Juan
J
Li
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
Dongxia
D
Hao
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China.
Zhiguo
Z
Su
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China. ghma@ipe.ac.cn.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China. ghma@ipe.ac.cn.
Guanghui
G
Ma
D004800:Enzymes, Immobilized / Q000737:chemistry*; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry*; D008863:Microspheres; D011109:Polymethacrylic Acids / Q000737:chemistry*; D016062:Porosity
D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D011109:Polymethacrylic Acids; C524317:poly(styrene-co-glycidyl methacrylate); C042535:polyglycidyl methacrylate; C093667:Novozyme 435; D008049:Lipase
Covalent binding; Gigaporous microspheres; Hydrophobicity; Lipase immobilization; Physical adsorption