Plasma metabolomics profiling for the prediction   of cytomegalovirus DNAemia and analysis of   virus–host interaction in allogeneic stem cell   transplant recipients.
Metabolomics analysis of biofluids is increasingly being recognized as a useful tool for the diagnosis and management of a number of infectious diseases. Here we showed that plasma metabolomics profiling by untargeted 1H nuclear magnetic resonance may allow the anticipation of the occurrence of cytomegalovirus (CMV) DNAemia in allogeneic stem cell transplant. For this purpose, key discriminatory metabolites were total glutathione, taurine, methylamine, trimethylamine N-oxide and lactate, all of which were upregulated in patients eventually developing CMV DNAemia. The overall classification accuracy (predictability) of the projection to latent structure discriminant analysis (PLS-DA) model in cross-validation technical replicates was 73 %. Increased levels of alanine, lactate and total fatty acids, and a shift in the fatty acid profile towards unsaturated species, were observed in patients with detectable CMV DNA in plasma. The classification accuracy of this PLS-DA model in cross-validation technical replicates was 81 %. Plasma metabolomics profiling may prove useful for identifying patients at highest risk for CMV DNAemia thus allowing early inception of antiviral therapy.
Metabolomic and Molecular Image Laboratory, Fundación de Investigación INCLIVA, Valencia, Spain.
Daniel
D
Monleón
Microbiology Service, Hospital Clínico Universitario, Fundación de Investigación INCLIVA, Valencia, Spain.
Estela
E
Giménez
Microbiology Service, Hospital Clínico Universitario, Fundación de Investigación INCLIVA, Valencia, Spain.
Beatriz
B
Muñoz-Cobo
Metabolomic and Molecular Image Laboratory, Fundación de Investigación INCLIVA, Valencia, Spain.
José Manuel
JM
Morales
Hematalogy and Medical Oncology Service, Hospital Clínico Universitario, Fundación de Investigación INCLIVA, Valencia, Spain.|Department of Medicine, School of Medicine, University of Valencia, Valencia, Spain.
Carlos
C
Solano
Hematalogy and Medical Oncology Service, Hospital Clínico Universitario, Fundación de Investigación INCLIVA, Valencia, Spain.
Paula
P
Amat
Microbiology Service, Hospital Clínico Universitario, Fundación de Investigación INCLIVA, Valencia, Spain.|Department of Microbiology, School of Medicine, University of Valencia, Valencia, Spain.
David
D
Navarro
D000293:Adolescent; D000328:Adult; D000368:Aged; D015331:Cohort Studies; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D003586:Cytomegalovirus Infections / Q000097:blood* / Q000209:etiology / Q000821:virology; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006402:Hematologic Diseases / Q000150:complications / Q000628:therapy; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D055432:Metabolomics; D008875:Middle Aged; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D013234:Stem Cells / Q000821:virology*; D066027:Transplant Recipients / Q000706:statistics & numerical data; D014184:Transplantation, Homologous / Q000009:adverse effects; D055815:Young Adult
D004279:DNA, Viral