Pd-Ir Core-Shell Nanocubes: A Type of Highly Efficient and Versatile Peroxidase Mimic.
Peroxidase mimics with dimensions on the nanoscale have received great interest as emerging artificial enzymes for biomedicine and environmental protection. While a variety of peroxidase mimics have been actively developed recently, limited progress has been made toward improving their catalytic efficiency. In this study, we report a type of highly efficient peroxidase mimic that was engineered by depositing Ir atoms as ultrathin skins (a few atomic layers) on Pd nanocubes (i.e., Pd-Ir cubes). The Pd-Ir cubes exhibited significantly enhanced efficiency, with catalytic constants more than 20- and 400-fold higher than those of the initial Pd cubes and horseradish peroxidase (HRP), respectively. As a proof-of-concept demonstration, the Pd-Ir cubes were applied to the colorimetric enzyme-linked immunosorbent assay (ELISA) of human prostate surface antigen (PSA) with a detection limit of 0.67 pg/mL, which is ∼110-fold lower than that of the conventional HRP-based ELISA using the same set of antibodies and the same procedure.
Department of Chemistry, Michigan Technological University , Houghton, Michigan 49931, United States.
Xiaohu
X
Xia
Department of Chemistry, Michigan Technological University , Houghton, Michigan 49931, United States.
Jingtuo
J
Zhang
Department of Materials Science and Engineering, University of Texas at Dallas , Richardson, Texas 75080, United States.
Ning
N
Lu
Department of Materials Science and Engineering, University of Texas at Dallas , Richardson, Texas 75080, United States.
Moon J
MJ
Kim
Department of Chemical Engineering, Louisiana State University , Baton Rouge, Louisiana 70803, United States.
Kushal
K
Ghale
Department of Chemical Engineering, Louisiana State University , Baton Rouge, Louisiana 70803, United States.
Ye
Y
Xu
Department of Chemistry, Michigan Technological University , Houghton, Michigan 49931, United States.
Erin
E
McKenzie
Department of Chemistry, Michigan Technological University , Houghton, Michigan 49931, United States.
Jiabin
J
Liu
Department of Chemistry, Michigan Technological University , Houghton, Michigan 49931, United States.
Haihang
H
Ye
D040761:Biomimetic Materials / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007495:Iridium / Q000737:chemistry*; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010165:Palladium / Q000737:chemistry*; D010544:Peroxidases / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000032:analysis*
D007495:Iridium; D010165:Palladium; D010544:Peroxidases; D017430:Prostate-Specific Antigen
catalytic efficiency; immunoassay; iridium; nanocrystal; peroxidase mimic