Hydroxyapatite-intertwined hybrid nanofibres for the mineralization of osteoblasts.
Advances in tissue engineering have enabled the development of bioactive composite materials to generate biomimetic nanofibrous scaffolds for bone replacement therapies. Polymeric biocomposite nanofibrous scaffolds architecturally mimic the native extracellular matrix (ECM), delivering tremendous regenerative potential for bone tissue engineering. In the present study, biocompatible poly(l-lactic acid)-co-poly(ε-caprolactone)-silk fibroin-hydroxyapatite-hyaluronic acid (PLACL-SF-HaP-HA) nanofibrous scaffolds were fabricated by electrospinning to mimic the native ECM. The developed nanofibrous scaffolds were characterized in terms of fibre morphology, functional group, hydrophilicity and mechanical strength, using SEM, FTIR, contact angle and tabletop tensile-tester, respectively. The nanofibrous scaffolds showed a higher level of pore size and increased porosity of up to 95% for the exchange of nutrients and metabolic wastes. The fibre diameters obtained were in the range of around 255 ± 13.4-789 ± 22.41 nm. Osteoblasts cultured on PLACL-SF-HaP-HA showed a significantly (p < 0.001) higher level of proliferation (53%) and increased osteogenic differentiation and mineralization (63%) for the inclusion of bioactive molecules SF-HA. Energy-dispersive X-ray analysis (EDX) data proved that the presence of calcium and phosphorous in PLACL-SF-HaP-HA nanofibrous scaffolds was greater than in the other nanofibrous scaffolds with cultured osteoblasts. The obtained results for functionalized PLACL-SF-HaP-HA nanofibrous scaffolds proved them to be a potential biocomposite for bone tissue engineering. Copyright © 2015 John Wiley & Sons, Ltd.
Centre for Nanofibres and Nanotechnology, Mechanical Engineering, Faculty of Engineering, National University of Singapore.
Andra
A
Sujana
Centre for Nanofibres and Nanotechnology, Mechanical Engineering, Faculty of Engineering, National University of Singapore.
Jayarama Reddy
JR
Venugopal
Centre for Nanofibres and Nanotechnology, Mechanical Engineering, Faculty of Engineering, National University of Singapore.
Bhaarathy
B
Velmurugan
Centre for Nanofibres and Nanotechnology, Mechanical Engineering, Faculty of Engineering, National University of Singapore.
Aleksander
A
Góra
Institute for Molecular Bioscience, Queensland Bioscience Precinct, University of Queensland, Brisbane, Australia.
Manohar
M
Salla
Centre for Nanofibres and Nanotechnology, Mechanical Engineering, Faculty of Engineering, National University of Singapore.
Seeram
S
Ramakrishna
D002113:Calcification, Physiologic; D002454:Cell Differentiation; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D054457:Tissue Scaffolds / Q000737:chemistry*
D017886:Durapatite
biocomposite; hyaluronic acid; hydroxyapatite; mineralization; osteoblasts; silk fibroin