Cocaine and metabolite concentrations in DBS and venous blood after controlled intravenous cocaine administration.
BACKGROUND
DBS are an increasingly common clinical matrix.


RESULTS
Sensitive and specific methods for DBS and venous blood cocaine and metabolite detection by LC-HRMS and 2D GC-MS, respectively, were validated to examine correlation between concentrations following controlled intravenous cocaine administration. Linear ranges from 1 to 200 µg/l were achieved, with acceptable bias and imprecision. Authentic matched specimens' (392 DBS, 97 venous blood) cocaine and benzoylecgonine concentrations were qualitatively similar, but DBS had much greater variability (21.4-105.9 %CV) and were lower than in blood.


CONCLUSIONS
DBS offer advantages for monitoring cocaine intake; however, differences between capillary and venous blood and DBS concentration variability must be addressed.
Chemistry & Drug Metabolism Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.|Program in Toxicology, University of Maryland Baltimore, Baltimore, MD 21201, USA.
Kayla N
KN
Ellefsen
Chemistry & Drug Metabolism Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.|Forensic Toxicology & Chemistry Laboratory, Criminalistics Institute of Sao Paulo, Sao Paulo, SP, Brazil.
Jose Luiz
JL
da Costa
Department of Sciences, John Jay College of Criminal Justice, City University of New York, New York, NY 10019, USA.
Marta
M
Concheiro
Chemistry & Drug Metabolism Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.
Sebastien
S
Anizan
Chemistry & Drug Metabolism Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.
Allan J
AJ
Barnes
Chemistry & Drug Metabolism Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.
Sandrine
S
Pirard
Department of Psychiatry, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
David A
DA
Gorelick
Chemistry & Drug Metabolism Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.
Marilyn A
MA
Huestis
D000328:Adult; D002853:Chromatography, Liquid / Q000379:methods; D003042:Cocaine / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000097:blood* / Q000378:metabolism; D059788:Dried Blood Spot Testing / Q000379:methods*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
C008671:norcocaine; C005618:benzoylecgonine; D003042:Cocaine