McCLEC, a robust and stable enzymatic based microreactor platform.
A microfluidic chip for cross-linked enzyme crystals (McCLEC) is presented and demonstrated to be a stable, reusable and robust biocatalyst-based device with very promising biotechnological applications. The cost-effective microfluidic platform allows in situ crystallization, cross-linking and enzymatic reaction assays on a single device. A large number of enzymatic reuses of the McCLEC platform were achieved and a comparative analysis is shown illustrating the efficiency of the process and its storage stability for more than one year.
Laboratorio de Estudios Cristalográficos, Laboratorio de Estudios Cristalográficos, IACT (CSIC-UGR), Avda de las Palmeras, 4, 18100 Armilla, Granada, Spain. jgavira@iact.ugr-csic.es.
Mayte
M
Conejero-Muriel
Isaac
I
Rodríguez-Ruiz
Sergio
S
Martínez-Rodríguez
Andreu
A
Llobera
José A
JA
Gavira
D000581:Amidohydrolases / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology; D055162:Biocatalysis; D057075:Enzyme Assays / Q000295:instrumentation*; D004795:Enzyme Stability; D056656:Lab-On-A-Chip Devices; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism
D009113:Muramidase; D000581:Amidohydrolases; C101846:formamidase