Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish.
During development, proliferation must be tightly controlled for organs to reach their appropriate size. While the Hippo signaling pathway plays a major role in organ growth control, how it senses and responds to increased cell density is still unclear. In this study, we use the zebrafish lateral line primordium (LLP), a group of migrating epithelial cells that form sensory organs, to understand how tissue growth is controlled during organ formation. Loss of the cell junction-associated Motin protein Amotl2a leads to overproliferation and bigger LLP, affecting the final pattern of sensory organs. Amotl2a function in the LLP is mediated together by the Hippo pathway effector Yap1 and the Wnt/β-catenin effector Lef1. Our results implicate for the first time the Hippo pathway in size regulation in the LL system. We further provide evidence that the Hippo/Motin interaction is essential to limit tissue size during development.
BIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.|Developmental Biology, Institute for Biology I, Faculty of Biology, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.
Sobhika
S
Agarwala
BIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.|Developmental Biology, Institute for Biology I, Faculty of Biology, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.
Sandra
S
Duquesne
BIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.|Image Analysis Lab, Institute for Computer Science, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.
Kun
K
Liu
BIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.|Image Analysis Lab, Institute for Computer Science, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.
Anton
A
Boehm
Developmental Biology, Institute for Biology I, Faculty of Biology, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.
Lin
L
Grimm
BIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.|Developmental Biology, Institute for Biology I, Faculty of Biology, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.
Sandra
S
Link
BIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.
Sabine
S
König
BIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.|ZBSA Center for Biological Systems Analysis, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.|Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.
Stefan
S
Eimer
BIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.|Image Analysis Lab, Institute for Computer Science, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.
Olaf
O
Ronneberger
Developmental Biology, Institute for Biology I, Faculty of Biology, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany.
Virginie
V
Lecaudey
D000091082:Angiomotins; D000818:Animals; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D008565:Membrane Proteins / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D000091102:YAP-Signaling Proteins; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development*; D029961:Zebrafish Proteins / Q000378:metabolism*
D000091082:Angiomotins; C507321:LEF1 protein, zebrafish; D008565:Membrane Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; C583457:Yes-associated protein (yap), zebrafish; D029961:Zebrafish Proteins; C519597:amotl2a protein, zebrafish
Amotl2; Hippo; Lef1; Yap1; developmental biology; organ size; proliferation; stem cells; zebrafish