Effect of osteoconductive hyaluronate hydrogels on calvarial bone regeneration.
BACKGROUND
Without exploitation of possibly immunogenic and carcinogenic bone morphogenetic protein, we developed simple but clinically feasible artificial bone graft using osteoconductive hyaluronate (HA) hydrogels and bioactive MegaGen synthetic bone (MGSB).


METHODS
HA hydrogels were synthesized by the crosslinking reaction between carboxyl groups of HA and amine groups of gelatin (GEL). Then, artificial bone grafts were prepared by mixing MGSB with HA-GEL hydrogels. The bone regeneration by the MGSB/HA-GEL hydrogel complex was assessed in the skull of New Zealand white male rabbits in 4 and 8 weeks.


RESULTS
HA hydrogels were synthesized by the crosslinking reaction between carboxyl groups of HA and amine groups of gelatin (GEL). Then, artificial bone grafts were prepared by mixing MGSB with HA-GEL hydrogels. In vitro proliferation of preosteogenic cells was enhanced with increasing molecular weight of HA. In addition, histological analysis of dissected tissues with hematoxylin and eosin staining confirmed the effective in vivo bone regeneration by the MGSB/HA-GEL hydrogel complex. The MGSB/HA-GEL hydrogels were well resorbed and partially substituted to the lamellar bone after implantation for 8 weeks.


CONCLUSIONS
The novel artificial bone graft of MGSB/HA-GEL hydrogel complex for effective bone regeneration might be clinically feasible for further development.
Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784 Korea.
Junseok
J
Yeom
Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784 Korea.
Byung Woo
BW
Hwang
MegaGen Research Institute of Science and Technology, 377-2 Gyochon-ri, Jain-myeon, Kyeongsan, Kyungbuk 712-852 Korea.
Dong Jun
DJ
Yang
Department of Oral Pathology, School of Dentistry, IHBR, Kyungpook National University, 188-1, Samdeok-dong, Jung-gu, Daegu, Kyungbuk 700-412 Korea.
Hong-In
HI
Shin
Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784 Korea.
Sei Kwang
SK
Hahn
Bone regeneration; Gelatin; Hyaluronic acid; Hydrogel; Synthetic bone