Correlation Analysis Between Expression Levels of Hepatic Growth Hormone Receptor, Janus Kinase 2, Insulin-Like Growth Factor-I Genes and Dwarfism Phenotype in Bama Minipig.
Animal growth and development are complex and sophisticated biological metabolic processes, in which genes plays an important role. In this paper, we employed real-time quantitative PCR (RT-qPCR) to analyze the expression levels of hepatic GHR, JAK2 and IGF-I genes in 1, 30, 180 day of Bama minipig and Landrace with attempt to verify the correlation between the expression of these growth-associated genes and the dwarfism phenotype of Bama minipig. The results showed that the expression levels of these 3 genes in Bama minipigs were down-regulated expressed from 1 day to 30 day, and which was up-regulated expressed in Landrace. The expression levels of the 3 genes on 1, 30, 180 day were prominently higher in Landrace than in Bama minipigs. The significant differences of the 3 genes expression levels on 1 day between this two breeds indicate that different expressions of these genes might occur before birth. It is speculated that the down-regulated expression of the 3 genes may have a close correlation with the dwarfism phenotype of Bama minipig. More investigations in depth of this study is under progress with the help of biochip nanotechnology.
Haowen
H
Yang
Qinyang
Q
Jiang
Dan
D
Wu
Ganqiu
G
Lan
Jing
J
Fan
Yafen
Y
Guo
Baojian
B
Chen
Xiurong
X
Yang
Hesheng
H
Jiang
D000375:Aging / Q000378:metabolism; D000818:Animals; D004392:Dwarfism / Q000503:physiopathology*; D018507:Gene Expression Regulation, Developmental; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D053614:Janus Kinase 2 / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D010641:Phenotype; D011986:Receptors, Somatotropin / Q000378:metabolism*; D013045:Species Specificity; D013552:Swine; D013556:Swine, Miniature / Q000502:physiology*
D011986:Receptors, Somatotropin; D007334:Insulin-Like Growth Factor I; D053614:Janus Kinase 2