Determination of hematocrit by voltage-induced hemolysis on a disposable electrochemical sensing strip.
Besides being a crucial parameter for surgery and clinical diagnosis, hematocrit tends to affect the analytical results of point-of-care analytical devices. Therefore, an accurate and quick method for measuring hematocrit was developed on the basis of screen-printed carbon electrodes (SPCE). An impulse DC voltage of 3.0 V was imposed on ferricyanide-coated SPCE to induce hemolysis, and the released hemoglobin reduced the ferricyanide, generating a higher oxidation current for estimating hematocrit. Hematocrit ranging from 10 to 70% can be determined in 5 s by linear sweep voltammetry (r(2) = 0.9907) or 0.8 s by 3 V of potential step voltammetry (r(2) = 0.9833).
Biosensor Lab, Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan, Republic of China. tzongjih@ntu.edu.tw.
Chih-Wei
CW
Weng
Bo-Chuan
BC
Hsieh
Yung-Te
YT
Hou
Tzong-Jih
TJ
Cheng
D001784:Blood Gas Analysis; D004209:Disposable Equipment; D004563:Electrochemistry / Q000295:instrumentation*; D006400:Hematocrit / Q000295:instrumentation*; D006461:Hemolysis; D006801:Humans