Interaction of Piriformospora indica with Azotobacter chroococcum.
Microbial communities in rhizosphere interact with each other and form a basis of a cumulative impact on plant growth. Rhizospheric microorganisms like Piriformospora indica and Azotobacter chroococcum are well known for their beneficial interaction with plants. These features make P. indica/A. chroococcum co-inoculation of crops most promising with respect to sustainable agriculture and to understanding the transitions in the evolution of rhizospheric microbiome. Here, we investigated interactions of P. indica with A. chroococcum in culture. Out of five Azotobacter strains tested, WR5 exhibited growth-promoting while strain M4 exerted growth-inhibitory effect on the fungus in axenic culture. Electron microscopy of co-culture indicated an intimate association of the bacterium with the fungus. 2-D gel electrophoresis followed by mass spectrometry of P. indica cellular proteins grown with or without WR5 and M4 showed differential expression of many metabolic proteins like enolase-I, ureaseD, the GTP binding protein YPT1 and the transmembrane protein RTM1. Fungal growth as influenced by bacterial crude metabolites was also monitored. Taken together, the results conform to a model where WR5 and M4 influence the overall growth and physiology of P. indica which may have a bearing on its symbiotic relationship with plants.
Applied Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India.
Soubhagya
S
Kumar Bhuyan
Amity Institute of Microbial Technology, Amity University, Noida-201303, Uttar Pradesh, India.
Prasun
P
Bandyopadhyay
Applied Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India.
Pramod
P
Kumar
Applied Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India.
Deepak
D
Kumar Mishra
Applied Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India.
Ramraj
R
Prasad
Applied Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India.
Abha
A
Kumari
Amity Institute of Biotechnology, Amity University, Noida-201301, Uttar Pradesh, India.
Kailash
K
Chandra Upadhyaya
Amity Institute of Microbial Technology, Amity University, Noida-201303, Uttar Pradesh, India.
Ajit
A
Varma
Applied Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India.
Pramod
P
Kumar Yadava
D001395:Azotobacter / Q000502:physiology* / Q000648:ultrastructure; D001487:Basidiomycota / Q000502:physiology* / Q000648:ultrastructure; D005656:Fungal Proteins / Q000378:metabolism; D056265:Microbial Interactions; D020543:Proteome; D040901:Proteomics / Q000379:methods; D064210:Secondary Metabolism
D005656:Fungal Proteins; D020543:Proteome