Antimicrobial application of nanofibrous mats self-assembled with quaternized chitosan and soy protein isolate.
Positively charged N-[(2-hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTCC) and negatively charged soy protein isolate (SPI) were alternately assembled on cellulose acetate (CA) electrospun nanofibrous mats via electrostatic layer-by-layer self-assembly technique. CA nanofibrous mats coated with bilayers of HTCC and SPI possessed more orderly-arranged structure than uncoated CA mats according to the observation from scanning electron microscopy images. The average diameter of the nanofibers was enlarged by the increase of the bilayer number. X-ray photoelectron spectroscopy indicated that HTCC and SPI were coated on the surface of the CA mats successfully. The average diameters of inhibition zones of (HTCC/SPI)10.5-films-coated nanofibrous mats against Escherichia coli and Staphylococcus aureus were 9.6mm and 11.53mm, respectively, which demonstrated the highest antimicrobial activity among samples in presented study.
Department of Environmental Science, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China; College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
Yijun
Y
Pan
College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
Xingjian
X
Huang
Department of Environmental Science, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
Xiaowen
X
Shi
Department of Environmental Science, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
Yingfei
Y
Zhan
College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
Gang
G
Fan
College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
Siyi
S
Pan
College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: jtian.jtian@gmail.com.
Jing
J
Tian
Department of Environmental Science, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China. Electronic address: hbdeng@whu.edu.cn.
Hongbing
H
Deng
Department of Environmental Science, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
Yumin
Y
Du
D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry*; D004355:Drug Stability; D004926:Escherichia coli / Q000187:drug effects; D057139:Nanofibers / Q000737:chemistry*; D030262:Soybean Proteins / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature
D000890:Anti-Infective Agents; D030262:Soybean Proteins; D048271:Chitosan
Antimicrobial activity; HTCC; Layer-by-layer; Nanofibrous mats; Soy protein isolate