A vector system for efficient and economical switching of a ura4(+) module to three commonly used antibiotic marker cassettes in Schizosaccharomyces pombe.
We describe here the development of a set of plasmid vectors that allow simple, efficient and economical switching of a ura4(+) module in existing Schizosaccharomyces pombe strains to any of the three routinely used antibiotic marker cassettes, kanMX6, hphMX6 and natMX6. In principle, the applications of this system can also be extended to switching ura4(+) for additional MX6 module-based cassettes, such as bleMX6, as long as the antibiotic marker has been cloned into an ura4(+) module-switching vector. We illustrate the application of this set of vectors in exchange of the ura4(+) marker in existing strains with three antibiotic marker cassettes with high efficiency.
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, People's Republic of China.
Yinghui
Y
Chen
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, People's Republic of China.
Lihua
L
Chen
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, People's Republic of China.
Ke
K
An
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, People's Republic of China.
Yamei
Y
Wang
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, People's Republic of China.
Quanwen
Q
Jin
D000935:Antifungal Agents / Q000494:pharmacology; D003001:Cloning, Molecular; D025141:Drug Resistance, Fungal / Q000235:genetics*; D005800:Genes, Fungal; D005819:Genetic Markers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D016681:Genome, Fungal; D010957:Plasmids / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics*; D014170:Transformation, Genetic
D000935:Antifungal Agents; D005819:Genetic Markers; D029702:Schizosaccharomyces pombe Proteins; C400810:ura4 protein, S pombe
Schizosaccharomyces; fission yeast; hphMX6; kanMX6; marker switch; natMX6; ura4+