Diospyros kaki Extract Inhibits Alkali Burn-Induced Corneal Neovascularization.
The purpose of this study was to evaluate the effect of ethanol extract of Diospyros kaki (EEDK) leaves on corneal neovascularization (CoNV) in rats. One week after the alkali burns in the corneas, the CoNV area coverage in the CoNV-positive control group, 100 mg/kg EEDK group, and 200 mg/kg EEDK group was 43.3% ± 5.5%, 337.7% ± 2.5%, and 27.2% ± 4.3%, respectively. The areas of CoNV in the EEDK-treated groups were significantly different from those of the CoNV group. EEDK significantly attenuated the upregulation of vascular endothelial growth factor, fibroblast growth factor, interleukin-6, and matrix metalloproteinase-2 (MMP-2) protein levels. Orally administrated D. kaki inhibited CoNV development in rats.
1 Department of Ophthalmology, University of Ulsan , Gangneung Asan Hospital, Gangneung, Gangwon-do, Korea.
Sung Jae
SJ
Yang
2 Natural Product Research Center, Korea Institute of Science and Technology (KIST) , Gangneung, Gangwon-do, Korea.
Hyoung
H
Jo
2 Natural Product Research Center, Korea Institute of Science and Technology (KIST) , Gangneung, Gangwon-do, Korea.|3 Department of Biological Chemistry, University of Science and Technology , Daejeon, Korea.
Kyung-A
KA
Kim
2 Natural Product Research Center, Korea Institute of Science and Technology (KIST) , Gangneung, Gangwon-do, Korea.
Hong Ryul
HR
Ahn
2 Natural Product Research Center, Korea Institute of Science and Technology (KIST) , Gangneung, Gangwon-do, Korea.
Suk Woo
SW
Kang
2 Natural Product Research Center, Korea Institute of Science and Technology (KIST) , Gangneung, Gangwon-do, Korea.|3 Department of Biological Chemistry, University of Science and Technology , Daejeon, Korea.
Sang Hoon
SH
Jung
D000468:Alkalies / Q000633:toxicity*; D000818:Animals; D002057:Burns, Chemical / Q000150:complications*; D016510:Corneal Neovascularization / Q000188:drug therapy* / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D029788:Diospyros / Q000737:chemistry*; D004195:Disease Models, Animal; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
D000468:Alkalies; D015850:Interleukin-6; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A; D020778:Matrix Metalloproteinase 2
Diospyros kaki; alkali burn; angiogenic factors; corneal neovascularization; eye diseases