Piezo-actuated parallel mechanism for biological cell release at high speed.
In this paper, a dynamic releasing approach is proposed for high-speed biological cell manipulation. A compact parallel mechanism for grasping and releasing microobjects is used to generate controllable vibration to overcome the strong adhesion forces between the end effector and the manipulated object. To reach the required acceleration of the end effector, which is necessary for the detachment of the target object by overcoming adhesion forces, vibration in the end effector is generated by applying sinusoidal voltage to the PZT actuator of the parallel mechanism. For the necessary acceleration, we focus on the possible range of the frequency of the PZT-actuator-induced vibration, while minimizing the amplitude of the vibration (14 nm) to achieve precise positioning. The effect of the air and liquid environments on the required vibration frequency for successful release is investigated. For the first time, release results of microbeads and biological cells are compared. Release of the biological cells with 100 % success rate suggests that the proposed active release method is an appropriate solution for adhered biological cells during the release task.
The Hamlyn Centre for Robotic Surgery, Imperial College London, London, SW7 2AZ, UK, ebu.avci@gmail.com.
Ebubekir
E
Avci
Takayuki
T
Hattori
Kazuto
K
Kamiyama
Masaru
M
Kojima
Mitsuhiro
M
Horade
Yasushi
Y
Mae
Tatsuo
T
Arai
D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D002469:Cell Separation / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D051379:Mice; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D008846:Micromanipulation / Q000295:instrumentation*; D008904:Miniaturization; D012371:Robotics / Q000295:instrumentation*; D014732:Vibration