Self-Organized Nanoparticles of Caffeic Acid Conjugated Polysaccharide and Its Anticancer Activity.
Caffeic acid-conjugated chitosan (ChitoCFA) and carboxymethyl dextran-b-poly(ethylene gycol) (CMD-PEG) copolymer were synthesized to fabricate self-organized nanoparticles. Nanoparticles were formed through ion-complex formation between ChitoCFA and CMD-PEG. Nanoparticles have small sizes ranged about 80 nm~300 nm with spherical shapes. Doxorubicin (DOX) was incorporated into the nanoparticles of ChitoCFA/CMD complexes. Particle sizes were increased according to the contents of drug. At drug release experiment, DOX was continuously released over 96 h. Anticancer acticity of nanoparticles were assessed with DOX-resistant CT26 cells. CT26 cells treated with DOX-incorporated nanoparticles revealed strong fluorescence intensity while free DOX revealed weak fluorescence intensity, indicating that DOX-loaded nanoparticles of ChitoCFA/CMD are promising vehicle for anticancer drug targeting.
Sang Joon
SJ
Lee
Ki-Choon
KC
Choi
Mi-Sun
MS
Kang
Jong-Suk
JS
Oh
Young-Il
YI
Jeong
Hyun Chul
HC
Lee
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage*; D000975:Antioxidants / Q000008:administration & dosage; D002109:Caffeic Acids / Q000008:administration & dosage; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004058:Diffusion; D004317:Doxorubicin / Q000008:administration & dosage; D006801:Humans; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry* / Q000648:ultrastructure; D058726:Nanoconjugates / Q000737:chemistry* / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D010316:Particle Size; D011092:Polyethylene Glycols; D016896:Treatment Outcome
D000975:Antioxidants; D002109:Caffeic Acids; D053769:Nanocapsules; D058726:Nanoconjugates; D011092:Polyethylene Glycols; D004317:Doxorubicin; D048271:Chitosan; C040048:caffeic acid