Frequency of Human Papillomavirus Genotypes 6, 11, 16, 18 And 31 in Paraffin-Embedded Tissue Samples of Invasive Breast Carcinoma, North- East of Iran.
Breast cancer is the most common female malignancy. Detection of DNA of human papillomaviruses (HPVs) in breast carcinomas suggests that the virus may play a role in the pathogenesis of this disease. The aim of this study was to evaluate the frequency of HPVs genotypes 6, 11, 16, 18 and 31 in paraffin-embedded tissue samples of invasive breast carcinomas.


METHODS
Three hundred and twenty six paraffin-embedded tissue samples of breast cancer were studied. PCR was performed using specific primers for HPV genotypes.


RESULTS
Of total 206 (63.2%) samples positive for Beta-globin gene, 54 (26.2%) were HPV-positive and 152 (73.8%) were negative for HPV. Distribution of HPV genotypes were as follows: 19 (25.7%) were positive for genotype 11, 5 (6.8%) were positive for genotype 6; and 2 cases (2.7%) were positive for both genotypes 6 and 11. Samples were also screened for HPV genotypes 16, 18 and 31 but none was positive.


CONCLUSIONS
The current study confirmed the association of HPV and breast cancer. However, all samples were negative for high-risk HPV types 16, 18 and 31.
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Masoumeh
M
Salehpour
Skin Diseases and Cutaneous Leishmanaisis Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Naser
N
Tayyebi Meibodi
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Roghayeh
R
Teimourpour
Dept. of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Adel
A
Ghorani-Azam
Dept. of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Samaneh
S
Sepahi
Dept. of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Sina
S
Rostami
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Zahra
Z
Meshkat
Breast Cancer; Human Papillomavirus (HPV); Polymerase Chain Reaction (PCR)