Identification of Soybean Proteins and Genes Differentially Regulated in Near Isogenic Lines Differing in Resistance to Aphid Infestation.
Soybean aphid is an important pest causing significant yield losses. The Rag2 locus confers resistance to soybean aphid biotypes 1 and 2. Transcriptomic and proteomic analyses were done over a 48 h period after aphid infestation using near isogenic lines (NILs) differing at the Rag2 locus. Comparing the Rag2 and/or rag2 lines identified 3445 proteins, of which 396 were differentially regulated between the two lines, including proteins involved in cell wall metabolism, carbohydrate metabolism, and stress response. RNA-seq transcriptomic analysis identified 2361 genes significantly regulated between the resistant and susceptible lines. Genes upregulated in the Rag2 line were annotated as being involved in cell wall, secondary, and hormone metabolism as well as in stress, signaling, and transcriptional responses. Genes downregulated in the Rag2 line were annotated as being involved in photosynthesis and carbon metabolism. Interestingly, two genes (unknown and mitochondrial protease) located within the defined Rag2 locus were expressed significantly higher in the resistant genotype. The expression of a putative NBS-LRR resistant gene within the Rag2 locus was not different between the two soybean lines, but a second NBL-LRR gene located just at the border of the defined Rag2 locus was. Therefore, this gene may be a candidate R gene controlling aphid resistance.
Laurent
L
Brechenmacher
Tran Hong Nha
TH
Nguyen
Ning
N
Zhang
USDA-ARS, Department of Horticulture and Crop Science, The Ohio State University , 1680 Madison Avenue, Wooster, Ohio 44691, United States.
Tae-Hwan
TH
Jun
Dong
D
Xu
USDA-ARS, Department of Horticulture and Crop Science, The Ohio State University , 1680 Madison Avenue, Wooster, Ohio 44691, United States.
M A Rouf
MA
Mian
Gary
G
Stacey
D000818:Animals; D001042:Aphids / Q000502:physiology; D002853:Chromatography, Liquid; D018506:Gene Expression Regulation, Plant / Q000276:immunology*; D063990:Gene Ontology; D056426:Genetic Loci; D018745:Genome, Plant; D005838:Genotype; D053858:Metabolic Networks and Pathways / Q000235:genetics / Q000276:immunology; D058977:Molecular Sequence Annotation; D057865:Plant Immunity / Q000235:genetics; D030821:Plants, Genetically Modified; D020543:Proteome / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D013025:Glycine max / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D053719:Tandem Mass Spectrometry
D020543:Proteome
NIL; Soybean; aphid; proteomic; resistant; susceptible; transcriptomic