[Identification of the 1RS-7DS.7DL wheat-rye small segment translocation lines].
Rye (Secale cereale L., RR) is a valuable genetic resource for the improvement of common wheat (Triticum aestivum L., AABBDD). Transferring alien rye genes into wheat by distant hybridization and automatic chromosome doubling is an important and efficient method to boost agronomic traits, disease resistance and widening the gene pool in wheat. In this study, an octoploid triticale CD-13 (AABBDDRR) was obtained via automatic chromosome doubling by crossing landrace Penganbaimaizi (T. aestivum L., AABBDD) and rye "Qinling rye" (S. cereale cv. Qinling, RR). GISH and FISH analyses indicated that CD-13 contained a 1RS-7DS.7DL wheat-rye small segment translocation chromosome. In order to transfer the 1RS-7DS small segment translocation into hexaploid wheat, 58 lines of the F5 inbred population from the cross CD-13 x Chuanmai 42 were screened for rye chromosome segments by GISH and FISH analyses. The results showed that 13 lines contained the 1RS-7DS.7DL small segment translocation chromosome by reciprocal translocation between 1RS and 7DS. These translocation lines carrying 1RS small rye alien segment were tested for the translocation breakpoints and the presence of a storage protein locus Sec-1. The Sec-1 locus was absent in the line 811, a stable 1RS-7DS.7DL small segment translocation line. The translocation breakpoint of 1RS-7DS.7DL of this line was located in the interval of IB267-IAG95 around the telomere of 1RS chromosome. Thousand-kernel weight of the line 811 was much higher than the parent CD-13, but not significantly different from Chuanmai 42. This indicated that 1RS-7DS.7DL small segment translocation had no negative effect on thousand-kernel weight in the genetic background of Chuanmai 42. The line with 1RS-7DS.7DL translocation chromosomes can be used as a new genetic material for further studies of valuable genes and their genetic effect on 1RS small segment.
Key Laboratory of Biology and Genetic Breeding in Wheat (Southwest) of Ministry of Agriculture, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.
Jun
J
Li
Key Laboratory of Biology and Genetic Breeding in Wheat (Southwest) of Ministry of Agriculture, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China;Key Laboratory of Plant Breeding and Genetics, College of Agronomy, Sichuan Agricultural University,Wenjiang 611130, China;
Xin-guo
XG
Zhu
Key Laboratory of Biology and Genetic Breeding in Wheat (Southwest) of Ministry of Agriculture, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.
Hong-shen
HS
Wan
Key Laboratory of Biology and Genetic Breeding in Wheat (Southwest) of Ministry of Agriculture, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.
Qin
Q
Wang
Key Laboratory of Plant Breeding and Genetics, College of Agronomy, Sichuan Agricultural University,Wenjiang 611130, China.
Zong-xiang
ZX
Tang
Key Laboratory of Plant Breeding and Genetics, College of Agronomy, Sichuan Agricultural University,Wenjiang 611130, China.
Shu-lan
SL
Fu
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
Zu-jun
Z
Yang
Key Laboratory of Biology and Genetic Breeding in Wheat (Southwest) of Ministry of Agriculture, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.
Man-yu
MY
Yang
Key Laboratory of Biology and Genetic Breeding in Wheat (Southwest) of Ministry of Agriculture, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.
Wu-yun
WY
Yang
D012434:Secale / Q000235:genetics*; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*