Period 1 and estrogen receptor-beta are downregulated in Chinese colon cancers.
To investigate whether Period 1 (PER1) and Estrogen receptor-beta (ER2) are associated with occurrence and development of Chinese colorectal cancers. By using RT-quantitative PCR, tissue microarray (TMA) and immunohistochemistry, we detected mRNA levels and protein levels of PER1 and ER2 in the cancerous tissues and paired normal adjacent tissues in patients with colorectal cancer. Survival analyses were performed by the Kaplan-Meier method utilizing log-rank test and univariate and multivariate Cox proportional modeling to measure 5-year disease-free survival (DFS) and overall survival (OS). Real-time PCR showed that, the delta Ct value (tumor tissue vs. normal mucosa) of PER1 or ER2 is 8.51 ± 2.81 vs. 7.34 ± 2.08 or 12.39 ± 2.43 vs. 9.76 ± 1.75, expression of PER1 and ER2 decreased significantly in tumor tissues compared with noncancerous mucosas of patients with or without metastasis (both of P values <0.001). Spearman test revealed that PER1 and ER2 were significantly down-regulated in cancerous tissues (r=0.283; P<0.001) which was also confirmed by immunohistochemistry of specimens from 203 colon cancer patients in a TMA format. The reduction of PER1 was associated with gender and distant metastasis (P=0.037 and P<0.001, respectively) whereas the decline of ER2 was associated with age (P=0.043) by analyzing the clinical data. However, we were not capable of detecting any association between PER1 level or ER2 level and overall survival (OS) or disease free survival (DFS). It is the first observation of correlated reduction of PER1 and ER2 in Chinese colon cancers, and they do play a certain role in colorectal cancer.
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Yupeng
Y
Wang
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Tonghai
T
Xing
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Li
L
Huang
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Guohe
G
Song
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Xing
X
Sun
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Lin
L
Zhong
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Junwei
J
Fan
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Dongwang
D
Yan
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Chongzhi
C
Zhou
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Feifei
F
Cui
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Fudong
F
Yu
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Jian
J
Chen
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Yang
Y
Yu
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Chao
C
Li
Department of Pathology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Huamei
H
Tang
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Zhihai
Z
Peng
Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University People's Republic of China.
Xiaoliang
X
Wang
D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D016009:Chi-Square Distribution; D002681:China; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000208:ethnology / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000601:surgery; D018450:Disease Progression; D018572:Disease-Free Survival; D015536:Down-Regulation; D047629:Estrogen Receptor beta / Q000032:analysis* / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D056950:Period Circadian Proteins / Q000032:analysis* / Q000235:genetics; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D012737:Sex Factors; D013997:Time Factors; D046888:Tissue Array Analysis; D016896:Treatment Outcome; D055815:Young Adult
D014408:Biomarkers, Tumor; C000600034:ESR2 protein, human; D047629:Estrogen Receptor beta; C539022:PER1 protein, human; D056950:Period Circadian Proteins; D012333:RNA, Messenger
Period 1; chronotherapy; colon Cancer; estrogen receptor-beta; prognosis