Hexane extract from Sargassum serratifolium inhibits the cell proliferation and metastatic ability of human glioblastoma U87MG cells.
The present study is the first to demonstrate the anticancer effects of a hexane extract from the brown algae Sargassum serratifolium (HES) on human cancer cell lines, including glioblastoma U87MG, cervical cancer HeLa and gastric cancer MKN-28 cells, as well as liver cancer SK-HEP 1 cells. Among these cancer cell lines, U87MG cells were most sensitive to the cell death induced by HES. HES exhibited a cytotoxic effect on U87MG cells at concentrations of 14-16 µg/ml, yet an effect was not observed in human embryonic kidney HEK293 cells. The antiproliferative effects of HES were regulated by inhibition of the MAPK/ERK signaling pathway which plays a pivotal role in the proliferation of glioblastoma U87MG cells. In addition, treatment with HES led to cell morphological changes and cell cytoskeleton degradation through regulation of actin dynamic signaling. Furthermore, migration and invasion of the U87MG cells were inhibited by HES via suppression of matrix metalloproteinase (MMP)-2 and -9 expression. Thus, our results suggest that HES is a potential therapeutic agent which has anticancer effects on glioblastoma.
Department of Microbiology, College of Natural Science, Pukyong National University, Busan 608-737, Republic of Korea.
Chang-Won
CW
Kang
Department of Microbiology, College of Natural Science, Pukyong National University, Busan 608-737, Republic of Korea.
Min-Seok
MS
Park
Department of Microbiology, College of Natural Science, Pukyong National University, Busan 608-737, Republic of Korea.
Nan-Hee
NH
Kim
Department of Microbiology, College of Natural Science, Pukyong National University, Busan 608-737, Republic of Korea.
Ji-Hyun
JH
Lee
Department of Marine Biology, College of Fisheries Science, Pukyong National University, Busan 608-737, Republic of Korea.
Chul-Woong
CW
Oh
Department of Food Science and Nutrition, College of Fisheries Science, Pukyong National University, Busan 608-737, Republic of Korea.
Hyeung-Rak
HR
Kim
Department of Microbiology, College of Natural Science, Pukyong National University, Busan 608-737, Republic of Korea.
Gun-Do
GD
Kim
D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005909:Glioblastoma / Q000188:drug therapy / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006586:Hexanes / Q000494:pharmacology*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D009362:Neoplasm Metastasis / Q000188:drug therapy; D044647:Sargassum / Q000737:chemistry*
D000970:Antineoplastic Agents; D006586:Hexanes