Antimicrobial susceptibility and molecular characterization of macrolide resistance of Mycoplasma bovis isolates from multiple provinces in China.
Mycoplasma bovis has spread widely throughout the world via animal movement and has become an important pathogen of bovine respiratory disease. However, the minimum inhibitory concentrations of antimicrobials for Mycoplasma bovis have not been studied in China. The objective of this study was to determine the prevalence and antibiotic resistance of Mycoplasma bovis isolated from young cattle with respiratory infection in China. Mycoplasma bovis was detected in 32/45 bovine respiratory infection outbreaks at beef farms in 8 provinces in China. The isolates were susceptible or had medium sensitivity to ciprofloxacin, enrofloxacin and doxycycline, but were frequently resistant to macrolides (13/32, 41%). An A2058G (Escherichia coli Numbering) mutation located in the rrnA operon in domain V of 23S rRNA was observed in strains that were resistant to macrolides. This single mutations at the rrnA operon in domain V of 23S rRNA may play an important role in the resistance of Mycoplasma bovis strains to macrolides.
College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street No.#2888, Changchun 130118, P.R. China.
Ling-Cong
LC
Kong
Duo
D
Gao
Bo-Yan
BY
Jia
Zi
Z
Wang
Yun-Hang
YH
Gao
Zhi-Hua
ZH
Pei
Shu-Ming
SM
Liu
Jiu-Qing
JQ
Xin
Hong-Xia
HX
Ma
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy / Q000382:microbiology; D002681:China; D024881:Drug Resistance, Bacterial / Q000235:genetics; D018942:Macrolides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D045682:Mycoplasma bovis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D012140:Respiratory Tract Diseases / Q000382:microbiology / Q000662:veterinary
D000900:Anti-Bacterial Agents; D018942:Macrolides