Mitogen-activated protein kinase 7 promotes cell proliferation, migration and invasion in SOSP-M human osteosarcoma cell line.
OBJECTIVE
Osteosarcoma (OS) is the most common primary bone tumor and has low cure rates. Our study aimed to evaluate the roles of mitogen-activated protein kinase 7 (MAPK7) in cell proliferation, migration and invasion using the SOSP-M human OS cell line as an in vitro model.


METHODS
SOSP-M cells were transfected with PCDNA3.1-MAPK7 and siRNA-MAPK7 plasmids using Lipofectamine 2000. Quantitative real-time polymerase chain reaction (RT-PCR) was performed to determine the relative expression level of MAPK7 and Western blot analysis was carried out to determine the expression level of ERK5 protein. Then MTT, scratch wound healing and Matrigel transwell assays were used to investigate the roles of MAPK7 expression in the proliferation, migration and invasion, respectively, of SOSP-M cells in vitro.


RESULTS
RT-PCR analysis showed that the expression level of MAPK7 increased significantly after transfection with PCDNA3.1-MAPK7 plasmid compared with the blank group, while it decreased significantly after transfection with siRNA-MAPK7 plasmid. Similar results for ERK5 expression were obtained by Western blot analysis. In addition, the cell proliferation rate, cell migration rate and invasive cell number in the PCDNA3.1-MAPK7 transfection group increased significantly compared with the blank group, while they decreased significantly in the siRNA-MAPK7 transfection group.


CONCLUSIONS
Our results indicate that overexpression of MAPK7 in human OS cells could promote cell proliferation, migration and invasion, whereas knockdown of MAPK7 expression had the opposite effect. All the results suggest that MAPK7 may serve as a potent target for drug development.
PLA Institute of Orthopedics, Beijing Army General Hospital, Beijing - P.R. China.
Yan
Y
Huang
PLA Institute of Orthopedics, Beijing Army General Hospital, Beijing - P.R. China.
Jianhua
J
Yao
PLA Institute of Orthopedics, Beijing Army General Hospital, Beijing - P.R. China.
Bing
B
Zhu
PLA Institute of Orthopedics, Beijing Army General Hospital, Beijing - P.R. China.
Jianzheng
J
Zhang
PLA Institute of Orthopedics, Beijing Army General Hospital, Beijing - P.R. China.
Tiansheng
T
Sun
D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D048054:Mitogen-Activated Protein Kinase 7 / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics
D034741:RNA, Small Interfering; C481581:MAPK7 protein, human; D048054:Mitogen-Activated Protein Kinase 7