14-3-3 λ protein interacts with ADF1 to regulate actin cytoskeleton dynamics in Arabidopsis.
Actin cytoskeleton dynamics is critical for variety of cellular events including cell elongation, division and morphogenesis, and is tightly regulated by numerous groups of actin binding proteins. However it is not well understood how these actin binding proteins are modulated in a physiological condition by their interaction proteins. In this study, we describe that Arabidopsis 14-3-3 λ protein interacted with actin depolymerizing factor 1 (ADF1) in plant to regulate F-actin stability and dynamics. Loss of 14-3-3 λ in Arabidopsis resulted in longer etiolated hypocotyls in dark and changed actin cytoskeleton architecture in hypocotyl cells. Overexpression of ADF1 repressed 14-3-3 λ mutant hypocotyl elongation and actin dynamic phenotype. In addition, the phosphorylation level of ADF1 was increased and the subcellular localization of ADF1 was altered in 14-3-3 λ mutant. Consistent with these observations, the actin filaments were more stable in 14-3-3 λ mutant. Our results indicate that 14-3-3 λ protein mediates F-actin dynamics possibly through inhibiting ADF1 phosphorylation in vivo.
Key Laboratory of Plant Stress, Life Science College, Shandong Normal University, Jinan, 250014, China.|State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
ShuangShuang
S
Zhao
Key Laboratory of Plant Stress, Life Science College, Shandong Normal University, Jinan, 250014, China. zhaoyx@sdnu.edu.cn.
YanXiu
Y
Zhao
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. guoyan@cau.edu.cn.
Yan
Y
Guo
D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism; D008841:Actin Cytoskeleton / Q000378:metabolism; D051339:Actin Depolymerizing Factors / Q000235:genetics / Q000378:metabolism; D000199:Actins / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D018546:Hypocotyl / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009154:Mutation; D010766:Phosphorylation; D019441:Plant Epidermis / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D030821:Plants, Genetically Modified; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction
D048948:14-3-3 Proteins; D051339:Actin Depolymerizing Factors; D000199:Actins; C588381:ADF1 protein, Arabidopsis; D029681:Arabidopsis Proteins; C117576:AFT1 protein, Arabidopsis
14-3-3; ADF; Arabidopsis; actin cytoskeleton dynamics; phosphorylation