A Novel Graphene Oxide-Based Protein Interaction Measurement Using Atomic Force Microscopy.
Graphene oxide (GO) is a promising material for biological applications because of its excellent physical/chemical properties such as aqueous processability, amphiphilicity, and surface functionalizability. Here we introduce a new biological application of GO, a novel GO-based technique for probing protein interactions using atomic force microscopy (AFM). GO sheets were intercalated between the protein-modified AFM probe and the polymer substrate in order to reduce the non-specific adhesion force observed during single-molecule force spectroscopy (SMFS). In this study, we used SMFS to probe the interaction of the actin filament and actin-related protein 2/3 complex (Arp2/3), an actin-binding protein. Our results confirm that the GO sheet reduces nonspecific adhesion of the probe to the substrate. Using the GO-based technique, we succeeded in estimating the dissociation constant of the actin filament-binding protein interaction.
Sung-Woong
SW
Han
Kyohei
K
Morita
Taiji
T
Adachi
D051376:Actin-Related Protein 2-3 Complex / Q000737:chemistry* / Q000648:ultrastructure; D000199:Actins / Q000737:chemistry* / Q000648:ultrastructure; D000268:Adhesiveness; D000327:Adsorption; D001665:Binding Sites; D006108:Graphite / Q000737:chemistry*; D008846:Micromanipulation / Q000379:methods; D018625:Microscopy, Atomic Force / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength
D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D010087:Oxides; D006108:Graphite