Electrogenerated chemiluminescence of tris(2,2' bipyridine)ruthenium(II) using common biological buffers as co-reactant, pH buffer and supporting electrolyte.
A series of aliphatic tertiary amines (HEPES, POPSO, EPPS and BIS-TRIS) commonly used to buffer the pH in biological experiments, were examined as alternative, non-toxic co-reactants for the electrogenerated chemiluminescence (ECL) of tris(2,2'-bipyridine)ruthenium(ii) ([Ru(bpy)3](2+)). These were found to be very attractive as "multi-tasking" reagents, serving not only as co-reactants, but also fulfiling the roles of pH buffer and supporting electrolyte within an aqueous environment; thus significantly simplifying the overall ECL analysis. Sub-nanomolar detection limits were obtained for [Ru(bpy)3](2+) in the presence of BIS-TRIS, making this species an valuable option for co-reactant ECL-based bioanalytical applications.
Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia. C.Hogan@latrobe.edu.au.
Noah
N
Kebede
Paul S
PS
Francis
Gregory J
GJ
Barbante
Conor F
CF
Hogan
D015082:2,2'-Dipyridyl / Q000737:chemistry; D000588:Amines; D002021:Buffers; D002623:Chemistry Techniques, Analytical; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004573:Electrolytes / Q000737:chemistry*; D006531:HEPES / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D049449:Luminescence; D008163:Luminescent Measurements; D008956:Models, Chemical; D010718:Phosphatidylserines / Q000737:chemistry; D010879:Piperazines / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry*; D012995:Solubility; D013451:Sulfonic Acids / Q000737:chemistry; D014325:Tromethamine / Q000031:analogs & derivatives / Q000737:chemistry
D000588:Amines; D002021:Buffers; D004573:Electrolytes; D007202:Indicators and Reagents; C000607446:N(2-hydroxythyl)piperazine-N'-(3-propanesulfonic acid); D010718:Phosphatidylserines; D010879:Piperazines; D013451:Sulfonic Acids; D014325:Tromethamine; C050294:1-palmitoyl-2-oleoylglycero-3-phosphoserine; D015082:2,2'-Dipyridyl; D012428:Ruthenium; C026272:Bistris; D006531:HEPES