Photodynamic Anticancer Activities of Multifunctional Cobalt Ferrite Nanoparticles in Various Cancer Cells.
To develop novel multifunctional magnetic nanoparticles (MNPs) with good magnetic properties, biocompatibility, and anticancer activities by photodynamic therapy (PDT), we synthesized multifunctional cobalt ferrite (CoFe2O4) nanoparticles (CoFe2O4-HPs-FAs) functionalized by coating them with hematoporphyrin (HP) for introducing photo-functionality and by conjugating with folic acid (FA) for targeting cancer cells. We evaluated the activities of the CoFe2O4-HPs-FAs by checking magnetic resonance imaging (MRI) in vitro, its biocompatibility, and photodynamic anticancer activities on FA receptor (FR)-positive and FR-negative cancer cell lines, Hela, KB, MCF-7, and PC-3 cells, to use for clinical applications. In this study, we have demonstrated that the CoFe2O4-HPs-FAs have good MRI and biocompatibility with non-cytotoxicity, and remarkable photodynamic anticancer activities at very low concentrations regardless of cell types. Particularly, the photo-killing abilities in 3.13 μg/mL of CoFe2O4-HPs-FAs were measured to be 91.8% (p < 0.002) for Hela, 94.5% (p < 0.007) for KB, 79.1% (p < 0.003) for MCF-7, and 71.3% (p < 0.006) for PC-3. The photodynamic anticancer activities in 6.25 and 12.5 μg/mL of CoFe2O4-HPs-FAs were measured to be over 95% (p < 0.004) to almost 100% regardless of cell types. The newly developed multifunctional CoFe2O4-HPs-FAs are effective for PDT and have potential as therapeutic agents for MRI-based PDT, because they have a high saturation value of magnetization and superparamagnetism.
Bong Joo
BJ
Park
Kyong-Hoon
KH
Choi
Ki Chang
KC
Nam
Anser
A
Ali
Joe Eun
JE
Min
Hyungbin
H
Son
Han S
HS
Uhm
Ho-Joong
HJ
Kim
Jin-Seung
JS
Jung
Eun Ha
EH
Choi
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D003035:Cobalt / Q000737:chemistry* / Q000627:therapeutic use; D005290:Ferric Compounds / Q000737:chemistry* / Q000627:therapeutic use; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000737:chemistry / Q000627:therapeutic use*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D005290:Ferric Compounds; D017319:Photosensitizing Agents; C001215:ferrite; D003035:Cobalt