Th17 and regulatory T cells contribute to the in situ immune response in skin lesions of Jorge Lobo's disease.
Jorge Lobo's disease (JLD) is a chronic granulomatous mycosis described in various Latin American countries. The main objective of the present study was to investigate the possible role of Th17 and Foxp3+ Treg cells in the pathogenesis of Jorge Lobo's disease. Human skin biopsies were submitted to an immunohistochemistry protocol to detect Foxp3, interleukin (IL)-1beta, CD25, IL-6, IL-17, and IL-23. The epidermis presented acanthosis, hyperkeratosis, and frequent presence of fungi. The dermis presented inflammatory infiltrate comprising macrophages, lymphocytes, epithelioid and multinucleated cells, and an intense number of fungi. Foxp3+ Treg cells and IL-17+ cells were visualized in lymphocytes in the inflammatory infiltrate. IL-1, IL-2R (CD25), IL-6, and IL-23 were visualized in the dermis, intermingled with fungal cells, permeating or participating of the granuloma. Following IL-17, the most prominent cytokine was IL-6. IL-23 and cells expressing CD25 were present in fewer number. The comparative analysis between IL-17 and Foxp3 demonstrated a statistically significant increased number of IL-17+ cells. Th17 cells play a role in the immune response of JLD. IL-1beta and IL-6 added to the previously described increased number of TGF-beta would stimulate such pattern of response. Th17 cells could be present as an effort to modulate the local immune response; however, high levels of a Th17 profile could overcome the role of Treg cells. The unbalance between Treg/Th17 cells seems to corroborate with the less effective immune response against the fungus.
Universidade de São Paulo, Faculdade de Medicina, Laboratório da Disciplina de Patologia de Moléstias Transmissíveis, São Paulo, SP, Brasil.
Luciane
L
Kanashiro-Galo
Universidade de São Paulo, Faculdade de Medicina, Laboratório da Disciplina de Patologia de Moléstias Transmissíveis, São Paulo, SP, Brasil Instituto de Assistência Médica ao Servidor Público Estadual, Programa de pós-graduação em Ciências da Saúde, São Paulo, SP, Brasil cpagliari@usp.br.
Carla
C
Pagliari
Universidade de São Paulo, Faculdade de Medicina, Laboratório da Disciplina de Patologia de Moléstias Transmissíveis, São Paulo, SP, Brasil Instituto de Assistência Médica ao Servidor Público Estadual, Programa de pós-graduação em Ciências da Saúde, São Paulo, SP, Brasil.
Tania Cristina
TC
Barboza
Nucleo de Medicina Tropical, Universidade Federal do Pará, Belém, PA, Brasil.
Arival Cardoso
AC
de Brito
Nucleo de Medicina Tropical, Universidade Federal do Pará, Belém, PA, Brasil Universidade do Estado do Pará, PA, Brasil.
Marilia Brasil
MB
Xavier
Nucleo de Medicina Tropical, Universidade Federal do Pará, Belém, PA, Brasil.
Clivia Maria Moraes
CM
de Oliveira
Nucleo de Medicina Tropical, Universidade Federal do Pará, Belém, PA, Brasil.
Deborah Aben Athar
DA
Unger
Universidade de São Paulo, Faculdade de Medicina, Laboratório da Disciplina de Patologia de Moléstias Transmissíveis, São Paulo, SP, Brasil.
Mirian Nacagami
MN
Sotto
Nucleo de Medicina Tropical, Universidade Federal do Pará, Belém, PA, Brasil Universidade do Estado do Pará, PA, Brasil.
Juarez Antonio Simões
JA
Quaresma
Universidade de São Paulo, Faculdade de Medicina, Laboratório da Disciplina de Patologia de Moléstias Transmissíveis, São Paulo, SP, Brasil.
Maria Irma Seixas
MI
Duarte
D001706:Biopsy; D005260:Female; D051858:Forkhead Transcription Factors / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D007378:Interleukins / Q000032:analysis; D060368:Lobomycosis / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000473:pathology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*
C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D007378:Interleukins
Jorge Lobo's Disease; Th17 cells; immune response; regulatory T cells