Association of HaeIII single nucleotide polymorphisms in the SLC2A1 gene with risk of diabetic nephropathy; evidence from Kurdish patients with type 2 diabetes mellitus.
OBJECTIVE
Given the growing rate of patients with type 2 diabetes mellitus, uncovering the effects of gene polymorphism on diabetes pathogenesis has attracted a lot of attention. Because glucose transporter 1 is involved in glucose uptake, the polymorphism of this gene may be an important risk factor in type 2 diabetes mellitus or in the progression of diabetes complications such as diabetic nephropathy. As far as the authors are concerned, this study is the first one aiming at evaluating the probable effects of solute carrier family 2 facilitated glucose transporter member 1 (SLC2A1) HaeIII polymorphism on clinical and laboratory outcomes of Kurdish patients with type 2 diabetes mellitus.


METHODS
This study was conducted involving 126 diabetic nephropathy patients and 150 diabetic patients without renal involvement. Serum levels of Cystatin C, fasting blood glucose, creatinine and urinary albumin; levels of glycated hemoglobin and estimated glomerular filtration rate were measured. Moreover, the Hae III polymorphism of SLC2A1 gene was determined by PCR-restriction fragment length polymorphism (RFLP).


RESULTS
The rate of CC genotype was higher (37%) in patients with diabetic nephropathy compared with controls. There were a significant correlation between the CC genotype and risk of diabetic nephropathy. There were significant correlations between genotypes, serum Cystatin C and estimated glomerular filtration rate in patients with diabetic nephropathy.


CONCLUSIONS
The results demonstrated the high frequency of C allele of SLC2A1 HaeIII in Kurdish patients with diabetic nephropathy. It was also found that this polymorphism is a significant risk factor for diabetic nephropathy. The effect of this polymorphism on clinical and laboratory characteristics of diabetic nephropathy patients was significant.
From the Department of Biology, Sanandaj Branch and.
Sabrieh
S
Amini
Department of Biochemistry, College of Science, Kurdistan and Research Branch, Islamic Azad University.
Mitra
M
Javanmardi
Cellular and Molecular Research Center and.
Aram
A
Mokarizadeh
Department of Biochemistry, College of Science, Kurdistan and Research Branch, Islamic Azad University.
Farzad
F
Maroofi
Department of Biochemistry, College of Science, Kurdistan and Research Branch, Islamic Azad University.
Chiya
C
Jalali
Department of Epidemiology and Biostatistics, Faculty of Medicine, Kurdistan University of Medical Sciences.
Namam-Ali
NA
Azadi
Laboratory of Health Center, State Prisons and Security and Corrective Measures Organization and.
Hamid
H
Mohammadi
Cellular and Molecular Research Center and Department of Clinical Biochemistry, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran abdi@muk.ac.ir.
Mohammad
M
Abdi
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology / Q000235:genetics*; D003928:Diabetic Nephropathies / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D051272:Glucose Transporter Type 1 / Q000235:genetics*; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors
D015415:Biomarkers; D051272:Glucose Transporter Type 1; D006442:Glycated Hemoglobin; C495677:SLC2A1 protein, human; C517652:hemoglobin A1c protein, human