Simulated microgravity affects ciprofloxacin susceptibility and expression of acrAB-tolC genes in E. coli ATCC25922.
As a representative fluoroquinolone antibacterial, ciprofloxacin is frequently used to treat infections caused by bacteria such as E. coli. It is much meaningful to explore ciprofloxacin susceptibility and investigate a possible mechanism of drug susceptibility changes in E. coli ATCC25922 exposed to the environmental stress of simulated microgravity. The subculture of E. coli lasted for 7 days under simulated microgravity conditions (SMG) and normal microgravity (NG) conditions. On the 8th day, the cultures were divided into three groups: (1) NG group (continuous NG cultures); (2) SMG group (continuous SMG cultures); (3) SMCNG group (simulated microgravity change into normal gravity cultures). Ciprofloxacin (a final concentration of 0.125 μg/ml) sensitivity and expression of acrAB-tolC genes were detected in E. coli cells. The count and percentage of viable cells in the SMG cultures bacteria exposed to ciprofloxacin were higher than that in NG cultures and reduced to the levels of NG group when they were subcultivated from SMG to NG. The expressions of efflux pump genes (acrA, acrB and tolC) were upregulated in SMG culture and downregulated to the levels of NG group when they were subcultivated from SMG to NG. Susceptibility to ciprofloxacin and expression of acrAB-tolC genes in E. coli could be reversibly affected by SMG conditions. Over expression of efflux pump genes acrAB-tolC perhaps played an important role in decreased CIP susceptibility under SMG.
School of Pharmaceutical Science, Peking University Beijing, China ; Special Medical Center, 306 Hospital of PLA Beijing, China.
Bingxin
B
Xu
Department of General Surgery, 306 Hospital of PLA Beijing, China.
Chenglin
C
Li
Special Medical Center, 306 Hospital of PLA Beijing, China.
Yanhua
Y
Zheng
Special Medical Center, 306 Hospital of PLA Beijing, China.
Shaoyan
S
Si
School of Pharmaceutical Science, Peking University Beijing, China.
Yuhua
Y
Shi
Department of General Surgery, 306 Hospital of PLA Beijing, China.
Yuling
Y
Huang
Department of Pathology, 306 Hospital of PLA Beijing, China.
Jianzhong
J
Zhang
Department of General Surgery, 306 Hospital of PLA Beijing, China.
Yan
Y
Cui
School of Pharmaceutical Science, Peking University Beijing, China.
Yimin
Y
Cui
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002939:Ciprofloxacin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D013997:Time Factors; D018474:Weightlessness Simulation
C000596829:AcrAB-TolC protein, E coli; D000900:Anti-Bacterial Agents; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D002939:Ciprofloxacin
E. coli; Simulated microgravity; acrAB-tolC genes; ciprofloxacin; drug susceptibility