Plasma ceramides are elevated in overweight Holstein dairy cows experiencing greater lipolysis and insulin resistance during the transition from late pregnancy to early lactation.
Insulin resistance is a homeorhetic adaptation to parturition in dairy cows transitioning from late pregnancy to early lactation. An increase in prepartum adiposity can predispose periparturient cows to greater lipolysis and insulin resistance, thus increasing the risk for metabolic disease. Mechanisms mediating the development of insulin resistance in overweight peripartal dairy cows may depend on ceramide metabolism. The sphingolipid ceramide accumulates in plasma and tissues of overweight monogastric animals, and facilitates saturated fatty acid-induced insulin resistance. Considering this evidence, we hypothesized that plasma ceramides would be elevated in periparturient dairy cattle and that these sphingolipids would correlate with the magnitude of lipolysis and insulin resistance. To test our central hypothesis, multiparous Holstein cows were allocated into 2 groups according to their body condition score (BCS) at d -30 prepartum: lean (BCS <3.0; n=10) or overweight (BCS >4.0; n=11). Blood samples were collected at d -45, -30, -15, and -7, relative to expected parturition, and at d 4 postpartum. Plasma glucose, insulin, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHBA) concentrations were measured, and insulin sensitivity was estimated. The concentrations of individual plasma ceramide and glycosylated ceramide were determined using liquid chromatography-based mass spectrometry. Results demonstrated that greater adiposity was associated with a greater loss in body condition during late pregnancy. Overweight cows had greater circulating concentrations of glucose, insulin, and NEFA, and lower insulin sensitivity relative to lean cows. We detected 30 different sphingolipids across 6 lipid classes with acyl chains ranging from 16 to 26 carbons. The most abundant plasma sphingolipids detected were C24:0-ceramide, C24:0-monohexosylceramide, and C16:0-lactosylceramide. Plasma concentrations of total ceramide and monohexosylceramide increased as lactation approached, and saturated ceramide and monohexosylceramide were elevated in cows with greater adiposity relative to those with a lean phenotype. Plasma ceramides (e.g., C24:0-ceramide) were positively correlated with plasma NEFA and inversely correlated with insulin sensitivity. Our data demonstrate a remodeled plasma sphingolipidome in dairy cows transitioning from late pregnancy to lactation characterized by a concomitant increase in plasma ceramides with the development of peripartal insulin resistance.
Division of Animal and Nutritional Sciences, West Virginia University, Morgantown 26505.
J E
JE
Rico
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21287.
V V R
VV
Bandaru
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21287.
J M
JM
Dorskind
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21287.
N J
NJ
Haughey
Division of Animal and Nutritional Sciences, West Virginia University, Morgantown 26505. Electronic address: JWMcFadden@mail.wvu.edu.
J W
JW
McFadden
D020155:3-Hydroxybutyric Acid / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001823:Body Composition; D002417:Cattle / Q000097:blood*; D002418:Cattle Diseases / Q000503:physiopathology; D002518:Ceramides / Q000097:blood*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology*; D007774:Lactation / Q000097:blood*; D008066:Lipolysis / Q000502:physiology*; D050177:Overweight / Q000503:physiopathology / Q000662:veterinary; D036801:Parturition / Q000378:metabolism; D049590:Postpartum Period / Q000378:metabolism; D011247:Pregnancy
D001786:Blood Glucose; D002518:Ceramides; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D020155:3-Hydroxybutyric Acid
ceramide; insulin resistance; periparturient dairy cow