A new DBS card with spot sizes independent of the hematocrit value of blood.
BACKGROUND
DBS cards have been a big promise for decades. However, blood with low hematocrit (Ht) values results on regular cellulose-based DBS cards in larger spot sizes, compared with blood with high Ht-values. A new material has been developed to solve this problem.


RESULTS
This material, based on hydrophilic-coated woven polyester fibers, shows spot sizes independent of the Ht-value of blood. Homogeneity over the spot is within 10% RSD.


CONCLUSIONS
Quantitative measurements over a broad Ht range show nonbiased results compared with whole spot analysis. The cards are experienced as reproducible, robust and easy to use on aspects of punchability and extractability.
DSM Resolve, Geleen, The Netherlands.
Ynze
Y
Mengerink
DSM Resolve, Geleen, The Netherlands.
John
J
Mommers
DSM Ahead, Geleen, The Netherlands.
Jun
J
Qiu
Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands.
Josh
J
Mengerink
Chemelot Campus, Geleen, The Netherlands.
Odile
O
Steijger
DSM Resolve, Geleen, The Netherlands.|Free University of Amsterdam, Amsterdam, The Netherlands.
Maarten
M
Honing
D000082:Acetaminophen / Q000097:blood; D002853:Chromatography, Liquid / Q000379:methods; D003061:Codeine / Q000097:blood; D059788:Dried Blood Spot Testing / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D006400:Hematocrit; D006801:Humans; D009020:Morphine / Q000097:blood; D018817:N-Methyl-3,4-methylenedioxyamphetamine / Q000097:blood; D011091:Polyesters / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods
D011091:Polyesters; D000082:Acetaminophen; D009020:Morphine; D018817:N-Methyl-3,4-methylenedioxyamphetamine; D003061:Codeine