Roles of miR-278-3p in IBP2 regulation and Bombyx mori cytoplasmic polyhedrosis virus replication.
Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is a major viral pathogen of silkworm and remains a big challenge to the sericultural industry. Insulin-related peptide binding protein 2 (IBP2) gene, induced by BmCPV infection may play an important role in B. mori immune response. MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at the post-transcriptional level and play an important role in various processes, including immunity and antiviral response. In this study, we identified IBP2 as one of the targets for miR-278-3p by using luciferase reporter assay. Overexpression of miR-278-3p negatively regulates the expression of IBP2 in silkworm larvae and positively regulates the mRNA transcript level of BmCPV. Our results suggest that miR-278-3p may play an important role in BmCPV replication. It's the first report on bmo-miRNAs in response to BmCPV infection and could provide a new clue to explore the molecular mechanism of BmCPV infection and host immunity.
Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, China. Electronic address: wp4114@163.com.
Ping
P
Wu
Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, China.
Guangxing
G
Qin
Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, China.
Heying
H
Qian
Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, China.
Tao
T
Chen
Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, China.
Xijie
X
Guo
D000818:Animals; D012831:Bombyx / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology*; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology*; D007814:Larva / Q000235:genetics / Q000276:immunology / Q000821:virology; D035683:MicroRNAs / Q000235:genetics / Q000276:immunology*; D012087:Reoviridae / Q000502:physiology*; D014779:Virus Replication / Q000235:genetics / Q000276:immunology*
D004268:DNA-Binding Proteins; D019476:Insect Proteins; D035683:MicroRNAs
Bombyx mori; Cytoplasmic polyhedrosis virus; IBP2; MicroRNA