Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo.
We evaluated the chondroprotective effects of wogonin by investigating its effects on the gene expression and production of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as on production of MMP-3 in the rat knee. Rabbit articular chondrocytes were cultured in a monolayer, and RT-PCR was used to measure interleukin-1β (IL-1β)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and type II collagen. In rabbit articular chondrocytes, the effects of wogonin on IL-1β-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of wogonin on MMP-3 protein production was also examined in vivo. In rabbit articular chondrocytes, wogonin inhibited the expression of MMP-3, MMP-1, MMP-13, and ADAMTS-4, but increased expression of type II collagen. Furthermore, wogonin inhibited the production and proteolytic activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.
Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Jin Sung
JS
Park
Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 301-131, Republic of Korea.
Hyun Jae
HJ
Lee
Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Dong Yeong
DY
Lee
Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Ho Seung
HS
Jo
Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Jin Hoon
JH
Jeong
Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Dong Hee
DH
Kim
Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Dae Cheol
DC
Nam
Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 301-131, Republic of Korea.
Choong Jae
CJ
Lee
Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Sun-Chul
SC
Hwang
Chondrocyte; Metalloproteinase; Osteoarthritis; Wogonin