PHYSICO2: an UNIX based standalone procedure for computation of physicochemical, window-dependent and substitution based evolutionary properties of protein sequences along with automated block preparation tool, version 2.
Automated genome sequencing procedure is enriching the sequence database very fast. To achieve a balance between the entry of sequences in the database and their analyses, efficient software is required. In this end PHYSICO2, compare to earlier PHYSICO and other public domain tools, is most efficient in that it i] extracts physicochemical, window-dependent and homologousposition-based-substitution (PWS) properties including positional and BLOCK-specific diversity and conservation, ii] provides users with optional-flexibility in setting relevant input-parameters, iii] helps users to prepare BLOCK-FASTA-file by the use of Automated Block Preparation Tool of the program, iv] performs fast, accurate and user-friendly analyses and v] redirects itemized outputs in excel format along with detailed methodology. The program package contains documentation describing application of methods. Overall the program acts as efficient PWS-analyzer and finds application in sequence-bioinformatics.


BACKGROUND
PHYSICO2: is freely available at http://sourceforge.net/projects/physico2/ along with its documentation at https://sourceforge.net/projects/physico2/files/Documentation.pdf/download for all users.
Authors equally contributed.
Shyamashree
S
Banerjee
Authors equally contributed.
Parth Sarthi Sen
PS
Gupta
Authors equally contributed.
Arnab
A
Nayek
Department of Biotechnology, The University of Burdwan, Golapbag, Burdwan, 713104, West Bengal, India.
Sunit
S
Das
Department of Biotechnology, The University of Burdwan, Golapbag, Burdwan, 713104, West Bengal, India.
Vishma Pratap
VP
Sur
Department of Biotechnology, The University of Burdwan, Golapbag, Burdwan, 713104, West Bengal, India.
Pratyay
P
Seth
Department of Biotechnology, The University of Burdwan, Golapbag, Burdwan, 713104, West Bengal, India.
Rifat Nawaz Ul
RN
Islam
Department of Biotechnology, The University of Burdwan, Golapbag, Burdwan, 713104, West Bengal, India.
Amal K
AK
Bandyopadhyay
ABPT; BLOCK; CYGWIN; FASTA; Program; Protein sequence