Anti-sickling Activity of Ursolic Acid Isolated from the Leaves of Ocimum gratissimum L. (Lamiaceae).
The present study reports in vitro anti-sickling activity and phytochemical analyses of the leaves of Ocimum gratissimum. Biological testing revealed that the plant extracts possess antisickling effects. The combination of spectroscopic techniques: 1D-NMR, 2D-NMR and MS revealed that ursolic acid is the major biologically active compound of O. gratissimum (Silva et al. in Molecules 13:2482-2487, 2008; Kedar et al. J Food Drug Anal 20:865-871, 2012). This study is the first report of the antisickling activity of ursolic acid isolated from O. gratissimum. The pharmaceutical relevance of findings from this study derives from the possibility of integrating O. gratissimum as an antisickling plant in the pharmacopoeia of Democratic Republic of the Congo. The identification of the active principle could enhance the standardization of antisickling recipe.
Faculté des Sciences, Université de Kinshasa, B.P. 190, Kinshasa XI, Democratic Republic of the Congo.
Dorothée Dinangayi
DD
Tshilanda
Faculté des Sciences, Université de Kinshasa, B.P. 190, Kinshasa XI, Democratic Republic of the Congo.
Damase NguwoVele
DN
Onyamboko
Faculté des Sciences, Université de Kinshasa, B.P. 190, Kinshasa XI, Democratic Republic of the Congo. pbabady@bilanutraceuticals.com.|Bila Nutraceuticals Company, 650 Portland Street Market, PO Box 26056, Dartmouth, NS, B2W 6P3, Canada. pbabady@bilanutraceuticals.com.
Philippe
P
Babady-Bila
Faculté des Sciences, Université de Kinshasa, B.P. 190, Kinshasa XI, Democratic Republic of the Congo.
Koto-Te-Nyiwa
KT
Ngbolua
Faculté des Sciences, Université de Kinshasa, B.P. 190, Kinshasa XI, Democratic Republic of the Congo.
Damien ShaTshibey
DS
Tshibangu
Faculté des Sciences, Université de Kinshasa, B.P. 190, Kinshasa XI, Democratic Republic of the Congo.|Institute of Natural Medicine, University of Toyama, 2630-Sugitani, Toyama, 930-0194, Japan.
Eddy
E
Dia Fita Dibwe
Faculté des Sciences, Université de Kinshasa, B.P. 190, Kinshasa XI, Democratic Republic of the Congo.
Pius Tshimankinda
PT
Mpiana
Antisickling activity; Ocimum gratissimum; Sickle cell disease; Ursolic acid