In vitro and in vivo anticancer effects of singly protonated dehydronorcantharidin silver coordination polymer in CT-26 murine colon carcinoma model.
Silver complexes are active constituents of the metal-based compounds; several studies suggest that silver complexes possess antimicrobial and anticancer properties. We have recently reported that Ag-SP-DNC, a novel silver and singly protonated dehydronorcantharidin complex, triggers oxidative stress-mediated apoptosis of lung cancer cells. In this study, we investigated the anticancer effects of Ag-SP-DNC in CT-26 murine colon carcinoma model. Ag-SP-DNC induced apoptosis of CT-26 cells, together with inhibition of cell proliferation; treatment of CT-26 tumor-bearing mice with Ag-SP-DNC delayed tumor growth. We also explored the mechanism of action of Ag-SP-DNC and found that Ag-SP-DNC treatment of CT-26 cells was associated with high levels of intracellular reactive oxygen species. The further experiments revealed that Ag-SP-DNC-treated cells underwent loss of mitochondrial membrane potential concomitant with intracellular calcium overload and caspase-3 activation. Taken together, our study demonstrates the potent anticancer effects of Ag-SP-DNC to colorectal cancer.
Department of Pharmacology, School of Medicine, Shandong University, Jinan 250012, China.
Xing
X
Jin
School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Jinan 250353, China.
Xuejie
X
Tan
Department of Pharmacology, School of Medicine, Shandong University, Jinan 250012, China.
Xiumei
X
Zhang
Cancer Therapy and Research Center, Shandong Provincial Hospital, Shandong University, Jinan 250021, China.
Mingyong
M
Han
Department of Pharmacology, School of Medicine, Shandong University, Jinan 250012, China. Electronic address: zhaoyunxue@sdu.edu.cn.
Yunxue
Y
Zhao
D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D051379:Mice; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011522:Protons; D017382:Reactive Oxygen Species / Q000378:metabolism; D013329:Structure-Activity Relationship
C000606696:Ag-SP-DNC; D000970:Antineoplastic Agents; D056831:Coordination Complexes; D009942:Organometallic Compounds; D011522:Protons; D017382:Reactive Oxygen Species
Ag-SP-DNC; Apoptosis; Colorectal cancer