Feeding probiotic Lactobacillus rhamnosus (MTCC 5897) fermented milk to suckling mothers alleviates ovalbumin-induced allergic sensitisation in mice offspring.
The neonatal period is often polarised to T helper (Th2) response at the time of birth, predisposing offspring to allergic disorders. Passive immunity through the mother's milk is critical for immune system development of newborns. Probiotics have been proposed to harmonise Th1/Th2 imbalance in allergic conditions in adults. In the present study, the anti-allergic effects of feeding probiotic Lactobacillus rhamnosus-fermented milk (PFM) either to dams during the suckling period or to their offspring after weaning individually or else in successive periods against ovalbumin (OVA)-induced allergy in newborns was analysed. After allergen sensitisation, physical symptoms of allergy, gut immune response, humoral immune response and cell-mediated response through interleukins were detected. Consumption of PFM by mothers and offspring showed a reduction (P<0·01) in physical allergic symptoms in newborns with an increase (P<0·01) in the numbers of goblet and IgA+ cells in the small intestine. Similarly, considerable (P<0·001) decreases in OVA-specific antibodies (IgE, IgG, IgG1) and ratios of IgE/IgG2a and IgG1/IgG2a in the sera of newborn mice were recorded. A decrease in IL-4 and an increase in interferon-γ levels further confirmed the shift from Th2 to Th1 pathway in PFM-fed mice. It is logical to conclude that the timing of PFM intervention in alleviating allergic symptoms is critical, which was found to be most effective when mothers were fed during the suckling period.
Animal Biochemistry Division,National Dairy Research Institute,Karnal 132001,Haryana,India.
Vamshi
V
Saliganti
Animal Biochemistry Division,National Dairy Research Institute,Karnal 132001,Haryana,India.
Rajeev
R
Kapila
Animal Biochemistry Division,National Dairy Research Institute,Karnal 132001,Haryana,India.
Rohit
R
Sharma
Animal Biochemistry Division,National Dairy Research Institute,Karnal 132001,Haryana,India.
Suman
S
Kapila
D000485:Allergens / Q000008:administration & dosage; D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D005285:Fermentation; D005512:Food Hypersensitivity / Q000517:prevention & control; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood / Q000276:immunology; D016753:Interleukin-10 / Q000097:blood / Q000276:immunology; D015847:Interleukin-4 / Q000097:blood / Q000276:immunology; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D052201:Lacticaseibacillus rhamnosus; D008297:Male; D051379:Mice; D008892:Milk / Q000737:chemistry*; D010047:Ovalbumin / Q000097:blood / Q000276:immunology*; D019936:Probiotics / Q000008:administration & dosage*; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000097:blood / Q000276:immunology
D000485:Allergens; D018926:Anti-Allergic Agents; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D007074:Immunoglobulin G; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D010047:Ovalbumin; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2
Allergies; COX-2 cyclo-oxygenase-2; Humoral immune response; IFN-γ interferon-γ; Lactobacilli; PFM probiotic fermented milk; Suckling–weaning transition; TLR-2 toll-like receptor-2; cfu colony-forming units