A two-photon activatable amino acid linker for the induction of fluorescence.
A new one- and two-photon activatable fluorophore based on ATTO565 was developed using a photolabile linker that simultaneously acts as a quencher. It is especially interesting for protein and peptide applications because it can be incorporated by standard peptide chemistry. The application of the new fluorogenic construct in super-resolution microscopy of antibody conjugates is shown.
Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Max-von-Laue-Str. 9, 60438 Frankfurt, Germany. heckel@uni-frankfurt.de.
Felix
F
Friedrich
Kathrin
K
Klehs
Manuela A H
MA
Fichte
Stephan
S
Junek
Mike
M
Heilemann
Alexander
A
Heckel
D000596:Amino Acids / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D017785:Photons
D000596:Amino Acids; D005456:Fluorescent Dyes