RNAi-mediated RPL34 knockdown suppresses the growth of human gastric cancer cells.
An increasing body of evidence suggests that ribosomal proteins may have ribosome-independent functions and may be involved in various physiological and pathological processes. To examine the role of ribosomal protein L34 (RPL34) in cancer transformation, we assessed its expression in gastric cancer cell lines and found it highly expressed. We further used lentivirus-mediated small interfering RNAs (siRNAs) to knockdown RPL34 expression in the human gastric cancer cell line SGC-7901. RNA interference (RNAi)-mediated inhibition of RPL34 expression in SGC-7901 cells significantly suppressed cell proliferation, increased apoptosis and arrested cells in the S phase. The results of the present study suggest that RPL34 plays a critical role in cell proliferation, cell cycle distribution and apoptosis of human malignant gastric cells.
Department of Oncology, Wannan Medical College, Wuhu, Anhui 241001, P.R. China.
Hui
H
Liu
Department of Oncology, Wannan Medical College, Wuhu, Anhui 241001, P.R. China.
Shaohua
S
Liang
Department of Oncology, Wannan Medical College, Wuhu, Anhui 241001, P.R. China.
Xi
X
Yang
Department of Medical Oncology, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China.
Zhaoning
Z
Ji
Department of Medical Oncology, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China.
Wenying
W
Zhao
Department of Medical Oncology, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China.
Xiaobing
X
Ye
Department of Medical Oncology, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China.
Jing
J
Rui
D017209:Apoptosis; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D034622:RNA Interference; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*
D012269:Ribosomal Proteins; C041672:ribosomal protein L34