Identification of a new antifungal oligoacetal derivative produced by Streptomyces toxytricini against Candida albicans.
Thirty actinomycete isolates were isolated from soil and tested against Candida albicans in vitro. The active isolate was identified by 16s-rRNA gene sequencing method as Streptomyces toxytricini. The antifungal compound was extracted with ethyl acetate followed by diethyl ether. Both HPLC and GC-MS analysis confirmed presence of one pure compound in the diethyl ether extract. The compound is a yellow liquid has a maximum absorbance at 240 nm in methanol. The chemical structure was elucidated by 1D and 2D-NMR and IR analyses. The elucidated molecular formula was C36H54O14. The compound is a polyacetal tricyclononane derivative, composed of a tricyclononane ring attached from the carbon atom number four with an oligo-acetal chain (six acetal groups in chain) and from the carbon atom number seven with a methoxy carbonyl benzene-1,3-dicarboxylic acid. The purposed name is: 4- {[tricycle(3.2.1.1(1,3))non-8-yl] methoxy carbonyl benzene-1,3-dicarboxylic acid} (2,4,5,6,7,8,9 heptaoxa, 3-ethoxy, 5,6,7,9-tetramethyl unidecane).
a College of Biotechnology , Misr University for Science and Technology (MUST) , 6th October City , Egypt.
Ahmed Z
AZ
Abdel Azeiz
b Biomedical Science Department , Zewail City , 6th October City , Egypt.
Donia K
DK
Hanafi
c Bioinformatics Department , Agriculture Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC) , Giza , Egypt.
Sameh E
SE
Hasanein
D039903:Actinobacteria / Q000737:chemistry; D000473:Alkanes; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification*; D002176:Candida albicans / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D013302:Streptomyces / Q000737:chemistry*
D000473:Alkanes; D000935:Antifungal Agents; C017573:nonane
Antifungal; Candida albicans; Streptomyces toxytricini; tricyclononane