Synthesis, characterization of dextran hydrogels and their in vitro release of gentamycin sulphate.
OBJECTIVE
This study reports on the synthesis and characterization of biodegradable dextran-allyl isocyanate-ethylamine (Dex-AE)/polyethylene glycol-diacrylate (PEGDA) hydrogels for the controlled release of gentamycin sulphate (GS) and in vitro inhibition of organisms.


METHODS
The Dex-AE precursor was prepared through a 2-step chemical modification and characterized by Fourier transform infrared spectroscopy (FTIR).


RESULTS
Scanning electron microscopy (SEM) results revealed that an increase in Dex-AE content led to an initial decrease in pore size of the Dex-AE/PEGDA hydrogels, but a further increase in Dex-AE content resulted in a slightly increase of pore size. The swelling data indicated that the swelling ratio depended on the precursor feed ratio. GS was incorporated into the hydrogels through 2 different methods, i.e., immersed and crosslinked. The crosslinked GS-Dex-AE/PEGDA hydorgels exhibited stronger antimicrobial activities against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Finally, the viscoelastic properties of crosslinked GS-Dex-AE/PEGDA hydorgels were investigated.
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou - China.|Department of Biomedical Engineering, Jinan University, Guangzhou - China.
Rui
R
Guo
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou - China.|Department of Biomedical Engineering, Jinan University, Guangzhou - China.
Peizhe
P
Chen
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou - China.|Department of Biomedical Engineering, Jinan University, Guangzhou - China.
Yunfei
Y
Mo
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou - China.|Department of Biomedical Engineering, Jinan University, Guangzhou - China.
Yong
Y
Lan
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou - China.|Department of Biomedical Engineering, Jinan University, Guangzhou - China.
Wei
W
Xue
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou - China.|Department of Chemistry, Jinan University, Guangzhou - China.
Yuanming
Y
Zhang
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry*; D005839:Gentamicins / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D011092:Polyethylene Glycols / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D003911:Dextrans; D005839:Gentamicins; D020100:Hydrogels; D011092:Polyethylene Glycols