Visualizing virus particle mobility in liquid at the nanoscale.
Currently, there remains a critical need to develop real-time imaging resources for life sciences. Here, we demonstrate the use of high resolution in situ imaging to observe biological complexes in liquid at the nanoscale. Using a model virus system, we produced the first time-resolved videos of individual biological complexes moving in solution within an electron microscope.
Virginia Tech Carilion Research Institute, Roanoke, VA 24016, USA. debkelly@vt.edu.
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A
Cameron Varano
Virginia Tech Carilion Research Institute, Roanoke, VA 24016, USA. debkelly@vt.edu.
Amina
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Rahimi
Application Science, Protochips, Inc., Raleigh, NC 27606, USA.
Madeline J
MJ
Dukes
Virginia Tech Carilion Research Institute, Roanoke, VA 24016, USA. debkelly@vt.edu.
Steven
S
Poelzing
Virginia Tech Carilion Research Institute, Roanoke, VA 24016, USA. debkelly@vt.edu.
Sarah
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M McDonald
Virginia Tech Carilion Research Institute, Roanoke, VA 24016, USA. debkelly@vt.edu.
Deborah F
DF
Kelly
D008854:Microscopy, Electron; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D012401:Rotavirus / Q000737:chemistry* / Q000302:isolation & purification; D013499:Surface Properties; D013997:Time Factors