Chemical characterization and in vitro antitumor activity of a single-component polysaccharide from Taxus chinensis var. mairei.
An alkali-soluble single-component polysaccharide, named CPTC-2, was isolated and purified from the leaves of Taxus chinensis var. mairei. by ion-exchange and gel-permeation chromatography in series. The weight-average molecular mass (Mw) of CPTC-2 was about 73.53kDa determined by gel permeation chromatography (GPC). The structural characteristics of CPTC-2 were analyzed by gas chromatography (GC), Infrared (IR) spectrum, nuclear magnetic resonance (NMR) spectroscopy, periodate oxidation and Smith degradation studies, as well as methylation analysis. The results showed that CPTC-2 consisted of glucose, mannose, xylose, arabinose, rhamnose, and galactose with a molar ratio of 1.00:0.32:0.27:3.34:1.22:1.84. CPTC-2 was mainly composed of one type of sugar, α-glycosidic linkage. It had a backbone composed of α-(1→3) Araf, α-(1→5) Araf and α-(1→4) Galp with branches composed of α-(1→3,5) Araf and β-(1→3,6) Manp. In vitro anti-tumor experiments with SGC-7901 cells were performed and assessed by MTS (MPEG-2 Transport Stream) method and flow cytometry. The results showed CPTC-2 could inhibit the growth of SGC-7901 cells in a concentration-dependent manner via increased apoptosis. The relationship between the structure of CPTC-2 and its anti-tumor activity indicated that the α-configuration glycosidic bond residues may be essential for the anti-tumor activity of this polysaccharide.
Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China; Zhejiang Key Laboratory of Antifungal Drugs, Taizhou 318100, China. Electronic address: wumb@zju.edu.cn.
Mianbin
M
Wu
Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: 21228180@zju.edu.cn.
Feifei
F
Zhang
Jinhua People's Hospital, Jinhua 321000, China. Electronic address: yuzp@163.com.
Zhangping
Z
Yu
Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: linjp@zju.edu.cn.
Jianping
J
Lin
Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: lryang@zju.edu.cn.
Lirong
L
Yang
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008745:Methylation; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D020946:Taxus / Q000737:chemistry*
D000970:Antineoplastic Agents; D011134:Polysaccharides; D010504:Periodic Acid
Antitumor; Characteristic; Isolation; Polysaccharide; Purification; Taxus chinensis var. mairei