MicroRNAs in avian influenza virus H9N2-infected and non-infected chicken embryo fibroblasts.
There is limited information about microRNAs (miR-NAs) in H9N2 subtype influenza virus-infected chicken cells or tissues. In this study, 10,487,469 and 13,119,795 reads were obtained from in-fected and non-infected chicken embryo fibroblasts, respectively. Seven hundred and thirty-six and 1004 miRNAs, including mature miRNAs and precursors, were obtained from the infected and non-infected fibro-blasts, respectively. Of those miRNAs, 48 were expressed differently between the groups: 37 had a low expression level in the infected chick-en embryo fibroblast, and the remaining 11 had a higher expression level. Every miRNA was predicted to target immune response-related genes. It has been found that some of the miRNAs, such as gga-miR-146c, gga-miR-181a, gga-miR-181b, gga-miR-30b, gga-miR-30c, gga-miR-30e, and gga-miR-455, are expressed differently in other types of influenza-infected chicken cells or tissues.
Animal Biotechnology Laboratory, Wuhan Institute of Animal and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan, China pengxiahao@163.com.
X
X
Peng
Animal Biotechnology Laboratory, Wuhan Institute of Animal and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan, China.
Q S
QS
Gao
Animal Biotechnology Laboratory, Wuhan Institute of Animal and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan, China.
L
L
Zhou
Animal Biotechnology Laboratory, Wuhan Institute of Animal and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan, China.
Z H
ZH
Chen
Animal Biotechnology Laboratory, Wuhan Institute of Animal and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan, China.
S
S
Lu
Animal Biotechnology Laboratory, Wuhan Institute of Animal and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan, China.
H J
HJ
Huang
Animal Biotechnology Laboratory, Wuhan Institute of Animal and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan, China.
C Y
CY
Zhan
Animal Biotechnology Laboratory, Wuhan Institute of Animal and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan, China.
M
M
Xiang
D000818:Animals; D001717:Birds / Q000821:virology; D002642:Chick Embryo; D005347:Fibroblasts / Q000821:virology; D015967:Gene Expression Regulation, Viral; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000235:genetics* / Q000821:virology; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*
D035683:MicroRNAs