Role of Autophagy in Capsaicin-Induced Apoptosis in U251 Glioma Cells.
In recent years, the role of capsaicin in cancer prevention and treatment has gained people's attention. However, the mechanism of anti-glioma cells by capsaicin has not been elucidated. Here, we discuss the mechanism of capsaicin in U251 cells. Cell viability was detected by MTT and extracellular LDH measurements, while immunofluorescence was performed to measure changes of LC3 in U251 cells. The expressions of LC3II, Puma-α, Beclin1, P62, Procaspase-3, and P53 were observed by immunoblotting. The cell viability decreased and the punctate patterns of LC3 in U251 cells were observed after Capsaicin treatment. Meanwhile, the expressions of Beclin1, P62, and Puma-α increased. After using 3-MA, the expressions of Beclin1 and Procaspase-3 were reduced while those of P53 and Puma-α increased. The expression of LC3II was increased after Pifithrin-α treatment. Therefore, we believed that capsaicin could induce apoptosis in U251 cells, and the inhibition of autophagy could contribute to apoptosis.
Experimental Teaching Center of Morphology, Xuzhou Medical College, Xuzhou, 221004, Jiangsu, China. ya-ping-liu@163.com.
Ya-Ping
YP
Liu
Research Center for Neurobiology, Department of Biology, Xuzhou Medical College, Xuzhou, 221004, Jiangsu, China.
Fu-Xing
FX
Dong
Research Center for Neurobiology, Department of Biology, Xuzhou Medical College, Xuzhou, 221004, Jiangsu, China.
Xiang
X
Chai
Research Center for Neurobiology, Department of Biology, Xuzhou Medical College, Xuzhou, 221004, Jiangsu, China.
Shuang
S
Zhu
Research Center for Neurobiology, Department of Biology, Xuzhou Medical College, Xuzhou, 221004, Jiangsu, China.
Bao-Le
BL
Zhang
Research Center for Neurobiology, Department of Biology, Xuzhou Medical College, Xuzhou, 221004, Jiangsu, China. dianshuaigao@163.com.
Dian-Shuai
DS
Gao
D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy / Q000187:drug effects*; D052160:Benzothiazoles / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D005910:Glioma / Q000188:drug therapy; D006801:Humans; D015398:Signal Transduction / Q000187:drug effects; D014050:Toluene / Q000031:analogs & derivatives / Q000494:pharmacology
D052160:Benzothiazoles; D014050:Toluene; C121565:pifithrin; C505407:CASP3 protein, human; D053148:Caspase 3; D002211:Capsaicin
Apoptosis; Autophagy; Capsaicin; Glioma