Synergistic effect of antioxidant system and osmolyte in hydrogen sulfide and salicylic acid crosstalk-induced heat tolerance in maize (Zea mays L.) seedlings.
Salicylic acid (SA), is a plant hormone with multifunction that is involved in plant growth, development and the acquisition of stress tolerance. Hydrogen sulfide (H2S) is emerging similar functions, but crosstalk between SA and H2S in the acquisition of heat tolerance is not clear. Our recent study firstly reported that SA treatment enhanced the activity of L-cysteine desulfhydrase (L-DES), a key enzyme in H2S biosynthesis, followed by induced endogenous H2S accumulation, which in turn improved the heat tolerance of maize seedlings. (1) In addition, NaHS, a H2S donor, enhanced SA-induced heat tolerance, while its biosynthesis inhibitor DL-propargylglycine (PAG) and scavenger hydroxylamine (HT) weakened SA-induced heat tolerance. Also, NaHS had no significant effect on SA accumulation and its biosynthesis enzymes phenylalanine ammonia lyase (PAL) and benzoic-acid-2-hydroxylase (BA2H) activities, as well as significant difference was not observed in NaHS-induced heat tolerance of maize seedlings by SA biosynthesis inhibitors paclobutrazol (PAC) and 2-aminoindan-2-phosph- onic acid (AIP) treatment. (1) Further study displayed that SA induced osmolytes (proline, betaine and trehalose) accumulation and enhancement in activity of antioxidant system in maize seedlings. These results showed that antioxidant system and osmolyte play a synergistic role in SA and H2S crosstalk-induced heat tolerance of maize seedlings.
a School of Life Sciences; Engineering Research Center of Sustainable Development and Utilization of Biomass Energy; Ministry of Education; Key Laboratory of Biomass Energy and Environmental Biotechnology; Yunnan Province; Yunnan Normal University ; Kunming , PR China.
Zhong-Guang
ZG
Li
D000222:Adaptation, Physiological; D000975:Antioxidants / Q000378:metabolism*; D001622:Betaine / Q000378:metabolism; D006358:Hot Temperature; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008954:Models, Biological; D011392:Proline / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism* / Q000494:pharmacology; D036226:Seedlings / Q000502:physiology*; D014199:Trehalose / Q000378:metabolism; D003313:Zea mays / Q000502:physiology*
D000975:Antioxidants; D001622:Betaine; D011392:Proline; D014199:Trehalose; D020156:Salicylic Acid; D006862:Hydrogen Sulfide
antioxidant system; heat tolerance; maize seedlings; osmolyte; salicylic acid; signal crosstalk; sodium hydrosulfide