Fucoidan - An α-amylase inhibitor from Sargassum wightii with relevance to NIDDM.
The present experiment was conducted to screen the α-amylase inhibitory activity of fucoidan extracted from Sargassum wightii collected at the coastal area of Mandapam, Tamil Nadu, India. Fucoidan was extracted from the sporophyll of S. Wightii by ethanol and CaCl2 precipitation method. The average yield was 1.8±0.16% and the extracted fucoidan was found to contain 53±0.52% of fucose and 36±0.60% of sulphate. Structural elucidation (FT-IR and NMR) and in vitro α-amylase activity of purified fucoidon were performed. Fucoidan at the concentration of 62.5, 125 and 250μg exhibited 24.81, 62.50 and 99.24% inhibition against α-amylase, respectively, in a dose dependent manner. Fucoidan from S. wightii also inhibits α-glucosidase which clearly indicates dual inhibitory activity of the compound. The IC50 value against α-amylase of fucoidan is found to be 103.83μg which is more effective than that of acarbose (16mg).
CMS College of Science and Commerce, Chinnavedampati (PO), Coimbatore, Tamil Nadu, India.
S
S
Lakshmana Senthil
CMS College of Science and Commerce, Chinnavedampati (PO), Coimbatore, Tamil Nadu, India. Electronic address: vinothbiochem@gmail.com.
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T
Vinoth Kumar
Dr. N.G.P. College of Arts and Science, Coimbatore, Tamil Nadu, India.
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D
Geetharamani
CMS College of Science and Commerce, Chinnavedampati (PO), Coimbatore, Tamil Nadu, India.
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Suja
CMS College of Science and Commerce, Chinnavedampati (PO), Coimbatore, Tamil Nadu, India.
Rincy
R
Yesudas
CMS College of Science and Commerce, Chinnavedampati (PO), Coimbatore, Tamil Nadu, India.
Amrutha
A
Chacko
D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
D011134:Polysaccharides; C007789:fucoidan; D000516:alpha-Amylases
Diabetes; Fuicodan; NIDDM; α-Amylase