Analytical and functional determination of polymyxin B protein binding in serum.
To enhance our understanding of the pharmacological properties of polymyxin B, serum protein binding for polymyxin B1, B2, and B3 and for isoleucine-polymyxin B1 was evaluated. Using equilibrium dialysis and ultrafiltration, comparable protein binding was found in all 4 components of polymyxin B (92% to 99%). Protein binding in human serum was further assessed using a functional assay, the results of which were in general agreement with previous findings (approximately 90%).
Department of Pharmacological and Pharmaceutical Sciences, University of Houston College of Pharmacy, Houston, Texas, USA.
Henrietta
H
Abodakpi
Department of Pharmacological and Pharmaceutical Sciences, University of Houston College of Pharmacy, Houston, Texas, USA.
Jie
J
Gohlke
Department of Pharmacy Practice and Translational Research, University of Houston College of Pharmacy, Houston, Texas, USA.
Kai-Tai
KT
Chang
Department of Pharmacological and Pharmaceutical Sciences, University of Houston College of Pharmacy, Houston, Texas, USA.
Diana S-L
DS
Chow
Department of Pharmacological and Pharmaceutical Sciences, University of Houston College of Pharmacy, Houston, Texas, USA Department of Pharmacy Practice and Translational Research, University of Houston College of Pharmacy, Houston, Texas, USA vtam@uh.edu.
Vincent H
VH
Tam
D006801:Humans; D011112:Polymyxin B / Q000097:blood* / Q000378:metabolism*; D011113:Polymyxins / Q000031:analogs & derivatives / Q000097:blood / Q000378:metabolism; D011485:Protein Binding
D011113:Polymyxins; C571510:polymyxin B2; C121746:polymyxin B(1); D011112:Polymyxin B