Comparison of Standard Versus Wide-Field Composite Images of the Corneal Subbasal Layer by In Vivo Confocal Microscopy.
OBJECTIVE
To evaluate whether the densities of corneal subbasal nerves and epithelial immune dendritiform cells (DCs) are comparable between a set of three representative standard images of in vivo confocal microscopy (IVCM) and the wide-field mapped composite IVCM images.


METHODS
This prospective, cross-sectional, and masked study included 110 eyes of 58 patients seen in a neurology clinic who underwent laser-scanning IVCM (Heidelberg Retina Tomograph 3) of the central cornea. Densities of subbasal corneal nerves and DCs were compared between the average of three representative standard images and the wide-field mapped composite images, which were reconstructed by automated mapping.


RESULTS
There were no statistically significant differences between the average of three representative standard images (0.16 mm2 each) and the wide-field composite images (1.29 ± 0.64 mm2) in terms of mean subbasal nerve density (17.10 ± 6.10 vs. 17.17 ± 5.60 mm/mm2, respectively, P = 0.87) and mean subbasal DC density (53.2 ± 67.8 vs. 49.0 ± 54.3 cells/mm2, respectively, P = 0.43). However, there were notable differences in subbasal nerve and DC densities between these two methods in eyes with very low nerve density or very high DC density.


CONCLUSIONS
There are no significant differences in the mean subbasal nerve and DC densities between the average values of three representative standard IVCM images and wide-field mapped composite images. Therefore, these standard images can be used in clinical studies to accurately measure cellular structures in the subbasal layer.
Ocular Surface Imaging Center, Cornea and Refractive Surgery Service, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States.
Ahmad
A
Kheirkhah
Ocular Surface Imaging Center, Cornea and Refractive Surgery Service, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States 2Boston Image Reading Center, Cornea Service, New England.
Rodrigo
R
Muller
NeuroCure Clinical Research Center, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Janine
J
Mikolajczak
Ocular Surface Imaging Center, Cornea and Refractive Surgery Service, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States.
Ai
A
Ren
NeuroCure Clinical Research Center, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Ella Maria
EM
Kadas
NeuroCure Clinical Research Center, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Hanna
H
Zimmermann
German Center for Neurodegenerative Diseases (DZNE), Berlin, Germany 6Department of Neurology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Harald
H
Pruess
NeuroCure Clinical Research Center, Charité-Universitätsmedizin Berlin, Berlin, Germany 6Department of Neurology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Friedemann
F
Paul
NeuroCure Clinical Research Center, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Alexander U
AU
Brandt
Ocular Surface Imaging Center, Cornea and Refractive Surgery Service, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States 2Boston Image Reading Center, Cornea Service, New England.
Pedram
P
Hamrah
D000328:Adult; D002452:Cell Count; D003315:Cornea / Q000166:cytology / Q000294:innervation*; D003430:Cross-Sectional Studies; D003712:Dendrites; D005260:Female; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods*; D008875:Middle Aged; D009882:Ophthalmic Nerve / Q000033:anatomy & histology*; D011446:Prospective Studies