Visualizing Cytoophidia Expression in Drosophila Follicle Cells via Immunohistochemistry.
We describe a user-friendly immunohistochemical approach for the detection of protein localization in Drosophila ovaries, here focusing on CTP synthase. This approach mainly uses fluorescently labeled antibodies to detect single, double, or multiple antigens. We provide a step-by-step protocol with detailed notes and tips, a simplified method that can also be adapted to detect protein localization beyond Drosophila ovaries.
MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX1 3PT, UK.
Ömür Y
ÖY
Tastan
Ji-Long
JL
Liu
D000818:Animals; D000906:Antibodies / Q000737:chemistry; D019731:Carbon-Nitrogen Ligases / Q000096:biosynthesis* / Q000302:isolation & purification; D004331:Drosophila melanogaster; D005260:Female; D007150:Immunohistochemistry / Q000379:methods*; D006080:Ovarian Follicle / Q000378:metabolism / Q000648:ultrastructure*
D000906:Antibodies; D019731:Carbon-Nitrogen Ligases; C019951:CTP synthetase