The roles of different salts and a novel osmotic pressure control strategy for improvement of DHA production by Schizochytrium sp.
The effects of different osmotic pressure, changed by six salts (NaCl, Na2SO4, (NH4)2SO4, KH2PO4 and MSG), on cell growth and DHA synthesis by Schizochytrium sp. were investigated. Six optimal mediums were obtained to study different osmotic pressure combinations at cell growth stage and DHA synthesis stage. Results showed that cultivated cell in higher osmotic pressure condition and fermented in lower osmotic pressure condition was benefit to enhance DHA synthesis. Combination 17-6 could get the maximum cell dry weight of 56.95 g/L and the highest DHA percentage in total fatty acids of 55.21%, while combination 17-B could get the highest lipid yield of 33.47 g/L with 42.10% DHA in total fatty acids. This was the first report about the enhancement of DHA production by osmotic regulation and this work provided two novel osmotic control processes for high lipid yield and high DHA percentage in total fatty acids.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu South Road, Nanjing, 211816, People's Republic of China.
Xue-Chao
XC
Hu
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu South Road, Nanjing, 211816, People's Republic of China.
Lu-Jing
LJ
Ren
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu South Road, Nanjing, 211816, People's Republic of China.
Sheng-Lan
SL
Chen
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu South Road, Nanjing, 211816, People's Republic of China.
Li
L
Zhang
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu South Road, Nanjing, 211816, People's Republic of China.
Xiao-Jun
XJ
Ji
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu South Road, Nanjing, 211816, People's Republic of China. biotech@njtech.edu.cn.
He
H
Huang
D003470:Culture Media / Q000737:chemistry*; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D009997:Osmotic Pressure; D058009:Stramenopiles / Q000378:metabolism*
D003470:Culture Media; D004281:Docosahexaenoic Acids
Docosahexaenoic acid; Osmotic pressure; Salts; Schizochytrium sp.