Isolation and Characterization of Bioactive Metabolites from Fruiting Bodies and Mycelial Culture of Ganoderma oerstedii (Higher Basidiomycetes) from Mexico.
Various species of the genus Ganoderma have been used for centuries according to oriental tradition as a source of medicines and nutrients. A chemical study of the fruiting bodies and mycelial culture of G. oerstedii was carried out with the idea of isolating and characterizing active natural components present to make use of their potential pharmaceutical application in Mexico. The fruiting bodies and mycelial culture of G. oesrtedii were lyophylized and extracted one after the other with hexane, chloroform, and methanol. Following this process, each substance was extracted separately by using column chromatography. From fruiting bodies eight metabolites, five sterols (ergosta-7,22-dien-3β-ol, ergosterol peroxide, ergosterol, cerevisterol, and ergosta-7,22-dien-3-one) as well as three terpene compounds (ganodermanondiol, ganoderic acid Sz, and ganoderitriol M) were obtained from fruiting bodies. From the mycelial culture three metabolites, two sterols (ergosterol and cerevisterol), and a new terpene compound (ganoderic acetate from the acid) were obtained. These structures were established based on a spectroscopic analysis mainly using nuclear magnetic resonance and a comparison with data already established.
Instituto de Biotecnologia y Ecologia Aplicada (INBIOTECA), Av. de las Culturas Veracruzanas No. 101, Col. E. Zapata C.P. 91090, Xalapa, Veracruz, Mexico; Laboratorio de Alta Tecnologia de Xalapa (LATEX), Universidad Veracruzana, Calle Medicos No. 5.
Guillermo
G
Mendoza
Centro de Investigaciones Cerebrales (CICE), Universidad Veracruzana. Av. Dr. Luis Castelazo Ayala s/n, Colonia Industrial Animas, 9119, Xalapa, Veracruz, Mexico.
Jorge
J
Suárez-Medellín
Laboratorio de Alta Tecnologia de Xalapa (LATEX), Universidad Veracruzana. Calle Medicos 5, Unidad del Bosque, 91010, Xalapa, Veracruz, Mexico.
César
C
Espinoza
LADISER Inmunologia y Biologia Molecular, Facultad de Ciencias Quimicas, Universidad Veracruzana, Orizaba, Veracruz, Mexico.
Angel
A
Ramos-Ligonio
Instituto Universitario de Bio-Organica "'Antonio Gonzalez"', Universidad de La Laguna, Astrofisico Francisco Sanchez 2, 38206, La Laguna, Tenerife, Spain.
José J
JJ
Fernández
Instituto Universitario de Bio-Organica "'Antonio Gonzalez"', Universidad de La Laguna, Astrofisico Francisco Sanchez 2, 38206, La Laguna, Tenerife, Spain.
Manuel
M
Norte
Laboratorio de Alta Tecnologia de Xalapa (LATEX), Universidad Veracruzana, Calle Medicos No. 5, Col. Unidad del Bosque, 91010, Xalapa, Veracruz, Mexico; Institute de Ciencias Basicas, Universidad Veracruzana, Xalapa, Veracruz, Mexico.
Ángel
Á
Trigos
D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D032902:Ganoderma / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008800:Mexico; D008958:Models, Molecular; D015394:Molecular Structure; D025282:Mycelium / Q000737:chemistry*; D013261:Sterols / Q000737:chemistry / Q000302:isolation & purification; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification
D001688:Biological Products; D013261:Sterols; D013729:Terpenes