Differential expression of glypican-3 (GPC3) in lung squamous cell carcinoma and lung adenocarcinoma and its clinical significance.
In this study, we examined the expression of glypican-3 (GPC3) in the 2 most common histological types of lung cancer, squamous cell carcinoma and adenocarcinoma, and explored the relationship between GPC3 expression and the prognosis of these 2 types of lung cancers. Lung cancer tissues and paracancerous tissues were collected from a total of 60 patients with lung squamous cell carcinoma or lung adenocarcinoma. GPC3 gene and protein expression in the tissue samples was examined using fluorescence-based real-time quantitative polymerase chain reaction, immunohistochemistry, and western blot analysis. In addition, the serological levels of GPC3 protein in lung cancer patients were analyzed using enzyme-linked immunosorbent assays. The overall expression of GPC3 protein in lung cancer was 45% (21/60). No GPC3 expression was detected in paracancerous lung tissues. Positive expression of GPC3 protein in lung squamous cell carcinoma was significantly higher than that in lung adenocarcinoma (70 vs 20%, P < 0.001). Among GPC3-positive lung squamous cell carcinoma and lung adenocarcinoma samples, samples collected from patients with lymph node metastasis and patients with poorly differentiated cancer exhibited more pronounced GPC-3 expression. GPC3 protein expression was significantly higher in lung squamous cell carcinoma than in lung adenocarcinoma. GPC3 may be a candidate marker for detecting lung squamous cell carcinoma.
Oncology Department, Gongli Hospital of Pudong New Area, Shanghai, China.
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X
Yu
Oncology Department, Gongli Hospital of Pudong New Area, Shanghai, China chenshi_wen3@163.com.
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Y
Li
Oncology Department, Gongli Hospital of Pudong New Area, Shanghai, China.
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SW
Chen
Oncology Department, Gongli Hospital of Pudong New Area, Shanghai, China.
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Y
Shi
Oncology Department, Gongli Hospital of Pudong New Area, Shanghai, China.
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F
Xu
D000230:Adenocarcinoma / Q000097:blood; D000077192:Adenocarcinoma of Lung; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053673:Glypicans / Q000097:blood / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
C105937:GPC3 protein, human; D053673:Glypicans; D012333:RNA, Messenger