The cariogenicity of hydrolyzed starch foods by measuring the polyacrylamide hydroxyapatite (PAHA) disc.
OBJECTIVE
This study measured the degree of demineralization of starch foods affected by hydrolyzed starch in total starch using the polyacrylamide hydroxyapatite (PAHA) disc.


METHODS
A total of 10 ml of test food was added to a PAHA disc and 5 ml artificial saliva, followed by inoculation with 1 μl of S. mutans and incubation at 37 °C for 180 min. The demineralization effects were then determined using CLSM.


RESULTS
The proportion of hydrolyzed starch in total starch in potato increased over time, while hydrolyzed starch in other test food decreased. When the amount of hydrolyzed starch in total starch increased after 180 min (p=0.000), the surface roughness of the PAHA disc in potato was significantly decreased. Wheat-based sugary snacks, such as chips and cookie, had relatively high cariogenic potentials, whereas baked potato had a relatively low cariogenic potential.


CONCLUSIONS
Hydrolyzed starch in total starch increased over time, and cariogenic potential was notably decreased. Measurement of PAHA disc may be a valid method for assessing the cariogenic potential of hydrolyzed starch foods.
Dept. of Preventive & Social Dentistry, School of Dentistry, Seoul National University, South Korea; Dept. of Food & Nutrition, College of Human Ecology, Seoul National University, South Korea.
Eun-Jung
EJ
Lee
Dept. of Food & Nutrition, College of Human Ecology, Seoul National University, South Korea.
In-Kyeong
IK
Hwang
Dept. of Preventive & Social Dentistry, School of Dentistry, Seoul National University, South Korea.
Dai-il
DI
Paik
Dept. of Preventive & Social Dentistry, School of Dentistry, Seoul National University, South Korea. Electronic address: jjbh@snu.ac.kr.
Bo-Hyoung
BH
Jin
D000180:Acrylic Resins / Q000737:chemistry*; D002326:Cariogenic Agents / Q000032:analysis*; D017886:Durapatite / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods; D005947:Glucose / Q000737:chemistry; D006868:Hydrolysis; D018613:Microscopy, Confocal; D062410:Snacks; D013213:Starch / Q000737:chemistry*; D013395:Sucrose / Q000737:chemistry; D017001:Tooth Demineralization; D000099166:Polyacrylamides
D000180:Acrylic Resins; D002326:Cariogenic Agents; D017886:Durapatite; D005947:Glucose; D013213:Starch; D013395:Sucrose; D000099166:Polyacrylamides
Cariogenicity; Confocal laser scanning microscopy (CLSM); Polyacrylamide hydroxyapatite (PAHA); Starch