Advances in synthetic dynamic circuits design: using novel synthetic parts to engineer new generations of gene oscillations.
As bioengineering applications expand, the need to design and implement circuits that exhibit dynamic properties increases. In particular, schemes that control precise patterns of gene expression as a function of time are essential for balancing multiple metabolic objectives in natural and synthetic systems. Given that modularity has been an important component of dynamic circuits, recent efforts to improve dynamic circuits have focused on replacing old parts for new components that increase the robustness, stability, and tunability. In this review, we show that incorporation of novel components such as regulatory noncoding RNAs (ncRNAs), promoter-transcription factor pairs, and metabolite sensors have allowed traditional dynamic circuits to obtain more robust functionality and improved dynamic properties.
Microbiology Graduate Program, University of Texas at Austin, 100 East 24th Street, A6500, Austin, TX 78712, USA.
Steven William
SW
Sowa
McKetta Department of Chemical Engineering, University of Texas at Austin, 200 East Dean Keeton Street, Stop C0400, Austin, TX 78712, USA.
Grant
G
Gelderman
McKetta Department of Chemical Engineering, University of Texas at Austin, 200 East Dean Keeton Street, Stop C0400, Austin, TX 78712, USA. Electronic address: lcontrer@che.utexas.edu.
Lydia Maria
LM
Contreras
D015870:Gene Expression; D005786:Gene Expression Regulation; D005818:Genetic Engineering; D006801:Humans; D011401:Promoter Regions, Genetic; D022661:RNA, Untranslated / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D022661:RNA, Untranslated; D014157:Transcription Factors