NCBI BLAST+ integrated into Galaxy.
BACKGROUND
The NCBI BLAST suite has become ubiquitous in modern molecular biology and is used for small tasks such as checking capillary sequencing results of single PCR products, genome annotation or even larger scale pan-genome analyses. For early adopters of the Galaxy web-based biomedical data analysis platform, integrating BLAST into Galaxy was a natural step for sequence comparison workflows.


RESULTS
The command line NCBI BLAST+ tool suite was wrapped for use within Galaxy. Appropriate datatypes were defined as needed. The integration of the BLAST+ tool suite into Galaxy has the goal of making common BLAST tasks easy and advanced tasks possible.


CONCLUSIONS
This project is an informal international collaborative effort, and is deployed and used on Galaxy servers worldwide. Several examples of applications are described here.
Information and Computational Sciences, James Hutton Institute, Invergowrie, Dundee, DD2 5DA Scotland UK.
Peter J A
PJ
Cock
Minnesota Supercomputing Institute, University of Minnesota, 599 Walter Library, 117 Pleasant St. SE, 55455 Minneapolis, MN USA.
John M
JM
Chilton
Department of Computer Science, Albert-Ludwigs-University of Freiburg, Georges-Köhler-Allee 106, Freiburg, 79110 Germany.
Björn
B
Grüning
Minnesota Supercomputing Institute, University of Minnesota, 599 Walter Library, 117 Pleasant St. SE, 55455 Minneapolis, MN USA.
James E
JE
Johnson
CRS4, Loc. Piscina Manna, 09010 Pula, CA Italy.
Nicola
N
Soranzo
D019295:Computational Biology; D020407:Internet; D009316:National Institutes of Health (U.S.); D014481:United States
Accessibility; Annotation; BLAST; Galaxy; Pipeline; Reproducibility; Sequence analysis; Workflow