Development of microwave assisted spectrophotometric method for the determination of glucose.
A spectrophotometric method was developed based on the microwave assisted synthesis of Maillard product. Various conditions of the reaction were optimized by varying the relative concentration of the reagents, operating temperature and volume of solutions used in the reaction in the microwave synthesizer. The absorbance of the microwave synthesized Maillard product was measured in the range of 360-740 nm using UV-Visible spectrophotometer. Based on the maximum absorbance, 370 nm was selected as the optimum wave length for further studies. The LOD and LOQ of glucose was found 3.08 μg mL(-1) and 9.33 μg mL(-1) with standard deviation of ±0.05. The developed method was also applicable to urine sample.
Department of Chemistry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.
Asif
A
Ali
Department of Chemistry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.
Zahid
Z
Hussain
Department of Chemistry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.
Muhammad Balal
MB
Arain
Department of Chemistry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.
Nasrullah
N
Shah
H. E. J. Research Institute of Chemistry, International Center for Chemical Sciences, University of Karachi, Karachi 75270, Pakistan.
Khalid Mohammad
KM
Khan
Department of Chemistry, Bacha Khan University, Charsada, Pakistan.
Hussain
H
Gulab
Department of Chemistry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.
Amir
A
Zada
D002021:Buffers; D005947:Glucose / Q000032:analysis*; D005998:Glycine / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015416:Maillard Reaction; D008872:Microwaves; D011786:Quality Control; D012015:Reference Standards; D012996:Solutions; D013053:Spectrophotometry / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D002021:Buffers; D012996:Solutions; D005947:Glucose; D005998:Glycine
Glucose; Glycine coupling; Maillard product analysis; Microwave assisted analysis