Mycofabricated biosilver nanoparticles interrupt Pseudomonas aeruginosa quorum sensing systems.
Quorum sensing (QS) is a chemical communication process that Pseudomonas aeruginosa uses to regulate virulence and biofilm formation. Disabling of QS is an emerging approach for combating its pathogenicity. Silver nanoparticles (AgNPs) have been widely applied as antimicrobial agents against human pathogenic bacteria and fungi, but not for the attenuation of bacterial QS. Here we mycofabricated AgNPs (mfAgNPs) using metabolites of soil fungus Rhizopus arrhizus BRS-07 and tested their effect on QS-regulated virulence and biofilm formation of P. aeruginosa. Transcriptional studies demonstrated that mfAgNPs reduced the levels of LasIR-RhlIR. Treatment of mfAgNPs inhibited biofilm formation, production of several virulence factors (e.g. LasA protease, LasB elastrase, pyocyanin, pyoverdin, pyochelin, rhamnolipid, and alginate) and reduced AHLs production. Further genes quantification analyses revealed that mfAgNPs significantly down-regulated QS-regulated genes, specifically those encoded to the secretion of virulence factors. The results clearly indicated the anti-virulence property of mfAgNPs by inhibiting P. aeruginosa QS signaling.
Centre of Excellence in Materials Science (Nanomaterials), Z.H. College of Engineering &Technology, Aligarh Muslim University, Aligarh-202002, India.
Braj R
BR
Singh
DSIR-recognized Research and Development Division, Sowbhagya Biotech Private Limited, Cherlapally, Hyderabad-500051, India.|Pharmacognosy &Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow-226001, India.
Brahma N
BN
Singh
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, India.
Akanksha
A
Singh
Centre of Excellence in Materials Science (Nanomaterials), Z.H. College of Engineering &Technology, Aligarh Muslim University, Aligarh-202002, India.
Wasi
W
Khan
Centre of Excellence in Materials Science (Nanomaterials), Z.H. College of Engineering &Technology, Aligarh Muslim University, Aligarh-202002, India.
Alim H
AH
Naqvi
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, India.
Harikesh B
HB
Singh
D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D018441:Biofilms / Q000187:drug effects; D005658:Fungi / Q000737:chemistry; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D008954:Models, Biological; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology*; D053038:Quorum Sensing / Q000187:drug effects*; D012834:Silver / Q000737:chemistry; D014031:Tobramycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D012834:Silver; D014031:Tobramycin