Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology.
Dogs with lymphoma are established as good model for human non-Hodgkin lymphoma studies. Canine cell lines derived from lymphomas may be valuable tools for testing new therapeutic drugs. In this context, we established a canine T-cell line, PER-VAS, from a primary aggressive T-cell lymphoma with large granular morphology. Flow cytometric analysis revealed a stable immunophenotype: PER-VAS cells were positively labelled for CD5, CD45, MHC II and TLR3, and were negative for CD3, CD4 and CD8 expression. Although unstable along the culture process, IL-17 and MMP12 proteins were detectable as late as at passages 280 and 325i.e. respectively 24 and 29 months post isolation. At passage 325, PER-VAS cells maintained the expression of IL-17, CD3, CD56, IFNγ and TNFα mRNAs as shown by RT-PCR analysis. Stable rearrangement of the TCRγ gene has been evidenced by PCR. PER-VAS cells have a high proliferation index with a doubling time of 16.5h and were tumorigenic in Nude mice. Compared to the canine cell lines already reported, PER-VAS cells display an original expression pattern, close to NKT cells, which makes them valuable tools for in vitro comparative research on lymphomas.
UPSP ICE 2011-03-101, Oncologie, VetAgro Sup, Campus Vétérinaire, 1 Avenue Bourgelat 69280 Marcy l'Etoile, France. Electronic address: catherine.bonnefont@vetagro-sup.fr.
Catherine
C
Bonnefont-Rebeix
UPSP ICE 2011-03-101, Oncologie, VetAgro Sup, Campus Vétérinaire, 1 Avenue Bourgelat 69280 Marcy l'Etoile, France.
Corinne
C
Fournel-Fleury
UPSP ICE 2011-03-101, Oncologie, VetAgro Sup, Campus Vétérinaire, 1 Avenue Bourgelat 69280 Marcy l'Etoile, France.
Frédérique
F
Ponce
UPSP ICE 2011-03-101, Oncologie, VetAgro Sup, Campus Vétérinaire, 1 Avenue Bourgelat 69280 Marcy l'Etoile, France.
Sara
S
Belluco
UPSP ICE 2011-03-101, Oncologie, VetAgro Sup, Campus Vétérinaire, 1 Avenue Bourgelat 69280 Marcy l'Etoile, France.
Dorothée
D
Watrelot
UPSP ICE 2011-03-101, Oncologie, VetAgro Sup, Campus Vétérinaire, 1 Avenue Bourgelat 69280 Marcy l'Etoile, France.
Sylvie E
SE
Bouteille
UPSP ICE 2011-03-101, Oncologie, VetAgro Sup, Campus Vétérinaire, 1 Avenue Bourgelat 69280 Marcy l'Etoile, France.
Sylvie
S
Rapiteau
Dendritics, 60 Avenue Rockefeller, 69008 Lyon, France.
Diane
D
Razanajaona-Doll
Dendritics, 60 Avenue Rockefeller, 69008 Lyon, France.
Jean-Jacques
JJ
Pin
INRA, UMR754 "Rétrovirus et Pathologie Comparée", Université Lyon 1, 50 Avenue Tony Garnier, 69007 Lyon, France.
Caroline
C
Leroux
UPSP ICE 2011-03-101, Oncologie, VetAgro Sup, Campus Vétérinaire, 1 Avenue Bourgelat 69280 Marcy l'Etoile, France.
Thierry
T
Marchal
D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D002460:Cell Line / Q000276:immunology* / Q000473:pathology; D004285:Dogs; D018703:Founder Effect; D015870:Gene Expression / Q000276:immunology*; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D051196:Toll-Like Receptor 3 / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
D015703:Antigens, CD; D020381:Interleukin-17; D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta; D051196:Toll-Like Receptor 3; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma
CD56 mRNA; Cell line; Dog; IL-17; T-cell lymphoma