Expression patterns of genes encoding plasma membrane aquaporins during fruit development in cucumber (Cucumis sativus L.).
Aquaporins are membrane channels precisely regulating water movement through cell membranes in most living organisms. Despite the advances in the physiology of fruit development, their participation during fruit development in cucumber still barely understood. In this paper, the expressions of 12 genes encoding plasma membrane intrinsic proteins (PIPs) were analyzed during cucumber fruit development in our work. Based on the homology search with known PIPs from rice, Arabidopsis and strawberry, 12 cucumber PIP genes subfamily members were identified. Cellular localization assays indicated that CsPIPs were localized in the plasma membrane. The qRT-PCR analysis of CsPIPs showed that 12 CsPIPs were differentially expressed during fruit development. These results suggest that 12 genes encoding plasma membrane intrinsic proteins (CsPIPs) play very important roles in cucumber life cycle and the data generated will be helpful in understanding their precise roles during fruit development in cucumber.
College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China.
Jin
J
Shi
College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China.
Jinfang
J
Wang
College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China.
Ren
R
Li
College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China.
Dianbo
D
Li
College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China.
Fengfeng
F
Xu
College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China.
Qianqian
Q
Sun
College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China.
Bin
B
Zhao
Beijing Key Lab of Vegetable Germplasm Improvement, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Key Lab of Biology & Genetic Improvement of Horticultural Crops (North China), MOA, China. Electronic address: maoaijun@nercv.org.
Ai-Jun
AJ
Mao
College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China. Electronic address: yaguo@cau.edu.cn.
Yang-Dong
YD
Guo
D000595:Amino Acid Sequence; D020346:Aquaporins / Q000737:chemistry / Q000235:genetics*; D018553:Cucumis sativus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling; D017343:Genes, Plant; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism
D020346:Aquaporins
Aquaporin; Cucumber; Fruit development; Plasma membrane intrinsic protein