High-throughput bacterial functional genomics in the sequencing era.
High-throughput functional genomic technologies are accelerating progress in understanding the diversity of bacterial life and in developing a systems-level understanding of model bacterial organisms. Here we highlight progress in deep-sequencing-based functional genomics, show how whole genome sequencing is enabling phenotyping in organisms recalcitrant to genetic approaches, recount the rapid proliferation of functional genomic approaches to non-growth phenotypes, and discuss how advances are enabling genome-scale resource libraries for many different bacteria.
Department of Microbiology and Immunology, University of California, San Francisco, CA 94158, USA.
Andrew N
AN
Gray
Department of Microbiology and Immunology, University of California, San Francisco, CA 94158, USA.
Byoung-Mo
BM
Koo
Graduate Group in Biophysics, University of California, San Francisco, CA 94158, USA.
Anthony L
AL
Shiver
Department of Microbiology and Immunology, University of California, San Francisco, CA 94158, USA.
Jason M
JM
Peters
Department of Microbiology and Immunology, University of California, San Francisco, CA 94158, USA.
Hendrik
H
Osadnik
Department of Microbiology and Immunology, University of California, San Francisco, CA 94158, USA; Department of Cell and Tissue Biology, University of California, San Francisco, CA 94158, USA. Electronic address: cgrossucsf@gmail.com.
Carol A
CA
Gross
D001419:Bacteria / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic / Q000379:methods; D014644:Genetic Variation; D016680:Genome, Bacterial; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D010641:Phenotype