MicroRNA-539 suppresses osteosarcoma cell invasion and migration in vitro and targeting Matrix metallopeptidase-8.
MicroRNAs (miRNA) are a class of small, non-coding RNA that involved in different cancer-related processes. Previous studies have been indicated miR-539 as a tumor suppressor during tumorigenesis. However, the role of miR-539 in osteosarcoma is still unclear. In this study, we demonstrate miR-539 was downregulated in osteosarcoma tissues compared to adjacent normal tissue. Functional study suggests miR-539 inhabits the osteosarcoma cell proliferation, invasion and migration. We also identified that MMP8 was a direct target of miR-539 by the luciferase activity assay. These findings provide evidence that miR-539 plays a key role in inhibiting osteosarcoma cell invasion and migration and can regulating MMP8 expression in osteosarcoma cells. These strongly suggest that exogenous miR-539 may have therapeutic value in treating osteosarcoma.
Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University Harbin 150001, China.
Hui
H
Jin
Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University Harbin 150001, China.
Wenbo
W
Wang
D020413:3' Untranslated Regions; D001859:Bone Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020784:Matrix Metalloproteinase 8 / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
D020413:3' Untranslated Regions; C552081:MIRN539 microRNA, human; D035683:MicroRNAs; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8
Matrix metallopeptidase-8 (MMP-8); miR-539; microRNA (miRNA); osteosarcoma