Hydroponic screening of black locust families for heavy metal tolerance and accumulation.
Present work examines phytoextraction potential of four black locust families (half-sibs 54, 56, 115, and 135) grown hydroponically. Plants were treated with 6 ppm of cadmium (Cd), 100 ppm of nickel (Ni), and 40 ppm of lead (Pb) added in Hoagland nutrient solution, accompanying with simultaneously applied all three metals. Responses to metals exposure among families were different, ranging from severe to slight reduction of root and shoot biomass production of treated plants. Calculated tolerance indices are indicating tested families as highly tolerant (Ti > 60). Family 135 had the lowest tolerance index, pointing that it was highly susceptible to applied metals. Comparing photosynthetic activities of tested families it has been noticed that they were highly sensitive to stress induced by heavy metals. Net photosynthetic rate of nickel treated plants was the most affected by applied concentration. Cadmium and nickel concentrations in stems and leaves of black locust families exceeded 100 mg Cd kg(-1) and 1000 mg Ni kg(-1), in both single and multipollution context. On the contrary, accumulation of lead in above ground biomass was highly affected by multipollution treatment. Tf and BCF significantly varied between investigated treatments and families of black locust. Concerning obtained results of heavy metals accumulation and tolerance of black locust families can be concluded that tested families might be a promising tool for phytoextraction purposes, but it takes to be further confirmed in field trials.
a University of Novi Sad, Faculty of Sciences , Department for Biology and Ecology , Novi Sad , Serbia.
Milan
M
Župunski
a University of Novi Sad, Faculty of Sciences , Department for Biology and Ecology , Novi Sad , Serbia.
Milan
M
Borišev
b University of Novi Sad, Institute of Lowland Forestry and Environment , Novi Sad , Serbia.
Saša
S
Orlović
a University of Novi Sad, Faculty of Sciences , Department for Biology and Ecology , Novi Sad , Serbia.
Danijela
D
Arsenov
a University of Novi Sad, Faculty of Sciences , Department for Biology and Ecology , Novi Sad , Serbia.
Nataša
N
Nikolić
b University of Novi Sad, Institute of Lowland Forestry and Environment , Novi Sad , Serbia.
Andrej
A
Pilipović
a University of Novi Sad, Faculty of Sciences , Department for Biology and Ecology , Novi Sad , Serbia.
Slobodanka
S
Pajević
D001673:Biodegradation, Environmental; D002104:Cadmium / Q000032:analysis / Q000378:metabolism*; D052918:Environmental Restoration and Remediation / Q000295:instrumentation / Q000379:methods*; D018527:Hydroponics; D007854:Lead / Q000032:analysis / Q000378:metabolism*; D009532:Nickel / Q000032:analysis / Q000378:metabolism*; D031301:Robinia / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*
D002104:Cadmium; D007854:Lead; D009532:Nickel
accumulation; black locust; cadmium; lead; nickel; photosynthesis