[Development of Selective LC Analysis Method for Biogenic and Related Compounds Based on a Fluorous Affinity Technique].
A separation-oriented derivatization method combined with LC has been developed for the selective analysis of biogenic and related compounds. In this method, we utilized a specific affinity between perfluoroalkyl-containing compounds, i.e., 'fluorous' compounds (fluorophilicity). Our strategy involves the derivatization of target analytes with perfluoroalkyl reagents, followed by selective retention of the derivatives with a perfluoroalkyl-modified stationary phase LC column. The perfluoroalkylated derivatives are strongly retained on the column owing to their fluorophilicity, whereas non-derivatized species, such as sample matrices, are hardly retained. Therefore, utilizing this derivatization method, target analytes can be determined selectively without interference from matrices. This method has been successfully applied to the LC analysis of some biogenic and related compounds in complex biological samples.
Faculty of Pharmaceutical Sciences, Fukuoka University.
Tadashi
T
Hayama
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis; D001679:Biogenic Amines / Q000032:analysis; D001681:Biological Assay / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D017611:Fluorine Compounds
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001679:Biogenic Amines; D017611:Fluorine Compounds