Delivery of Transferrin-Conjugated Polysaccharide Nanoparticles in 9L Gliosacoma Cells.
To investigate the possibility of drug targeting via the transferrin receptor-mediated pathway, iron-saturated transferrin was conjugated with chitosan (Tr-chitosan) and complexed with doxorubicin-conjugated methoxy poly(ethylene glycol)-b-dextran succinate (DEX-DOX). DEX-DOX nanoparticles have spherical morphologies with less than 150 nm particle sizes. When Tr-chitosan was complexed with DEX-DOX nanoparticles (TR nanoparticle), particle sizes were increased to higher than 200 nm. Viability of 9L cells with treatment of doxorubicin (DOX) or DEX-DOX nanoparticle was dose-dependently decreased regardless of transferrin receptor blocking. However, cytotoxicity of TR nanoparticles was reduced by blocking of transferrin receptor. Flow cytometric analysis and confocal microscopic observation showed that fluorescence intensity of tumor cells with treatment of TR nanoparticles was significantly decreased by blocking of transferring receptor while DEX-DOX nanoparticles were not affected by blocking of transferring receptor. These results indicated that TR nanoparticles are promising candidates for brain tumor drug delivery.
Young-Il
YI
Jeong
Young-Wook
YW
Kim
Shin
S
Jung
Jian
J
Pei
Min
M
Wen
Song-Yuan
SY
Li
Hyang-Hwa
HH
Ryu
Jung Cheol
JC
Lim
Woo-Youl
WY
Jang
In-Young
IY
Kim
Kyung-Sub
KS
Moon
Tae-Young
TY
Jung
D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D016503:Drug Delivery Systems; D004705:Endocytosis; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D010316:Particle Size; D051381:Rats; D011990:Receptors, Transferrin / Q000378:metabolism; D014168:Transferrin / Q000737:chemistry / Q000493:pharmacokinetics*
D003911:Dextrans; D011990:Receptors, Transferrin; D014168:Transferrin; D004317:Doxorubicin; D048271:Chitosan