Factors affecting the activation and inhibition of intracellular enzymes for degradation of 1,2 diamino benzene: kinetics and thermodynamic studies.
Citrobacter freundii, the bacterium isolated from marine sediments was capable of degrading 1,2 diamino benzene (DAB), an endocrine disruptor. The mixed intracellular enzymes from C. freundii were extracted and purified. The mixed intracellular enzymes were used for the degradation of DAB and degree of degradation was evaluated in terms of pyruvic acid, the end product, formed. The variables such as effect of pH, temperature and metal ions on the degradation of DAB using mixed intracellular enzymes (MICE) were investigated. The maximum amount of pyruvic acid formed was found to be 569 ± 5 µg with 96% degradation efficiency at pH 7; temperature 25 °C; zinc nitrate 0.1 mM; and copper sulphate ions 0.15 mM. The stability of MICE at different temperatures and the interaction of MICE with metal ions were confirmed using FT-IR spectroscopy. The formation of pyruvic acid from degradation of DAB followed pseudo-second-order rate kinetics and it was a spontaneous, exothermic process. The activation energy of degradation of DAB by MICE was found to be 82.55 kJ/mol.
Environmental Technology Division, Council of Scientific and Industrial Research (CSIR), Central Leather Research Institute (CLRI), Adyar, Chennai, Tamilnadu, 600 020, India. psaranyanarasimman@gmail.com.
Saranya
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Environmental Technology Division, Council of Scientific and Industrial Research (CSIR), Central Leather Research Institute (CLRI), Adyar, Chennai, Tamilnadu, 600 020, India. ganesansekaran@gmail.com.
Sekaran
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D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D016971:Citrobacter freundii / Q000201:enzymology*; D004789:Enzyme Activation / Q000502:physiology; D007700:Kinetics; D010655:Phenylenediamines / Q000378:metabolism*; D013816:Thermodynamics
D001426:Bacterial Proteins; D010655:Phenylenediamines; C034193:1,2-diaminobenzene
1,2 Diamino benzene; Citrobacter freundii; Metal ions; Mixed intracellular enzymes; Pyruvic acid