Exclusive Decoration of Simian Immunodeficiency Virus Env with High-Mannose Type N-Glycans Is Not Compatible with Mucosal Transmission in Rhesus Macaques.
Human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) envelope (Env) proteins are extensively decorated with N-glycans, predominantly of the high-mannose type. However, it is unclear how high-mannose N-glycans on Env impact viral spread. We show that exclusive modification of SIV Env with these N-glycans reduces viral infectivity and abrogates mucosal transmission, despite increasing viral capture by immune cell lectins. Thus, high-mannose N-glycans have opposed effects on SIV infectivity and lectin reactivity, and a balance might be required for efficient mucosal transmission.
Institute for Cellular Chemistry, Hannover Medical School, Hannover, Germany Infection Biology Unit, German Primate Center, Göttingen, Germany.
Christina B
CB
Karsten
Institute for Cellular Chemistry, Hannover Medical School, Hannover, Germany.
Falk F R
FF
Buettner
Max-Planck-Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.
Samanta
S
Cajic
Infection Biology Unit, German Primate Center, Göttingen, Germany.
Inga
I
Nehlmeier
Unit of Infection Models, German Primate Center, Göttingen, Germany.
Berit
B
Neumann
Unit of Infection Models, German Primate Center, Göttingen, Germany.
Antonina
A
Klippert
Unit of Infection Models, German Primate Center, Göttingen, Germany.
Ulrike
U
Sauermann
Max-Planck-Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.
Udo
U
Reichl
Institute for Cellular Chemistry, Hannover Medical School, Hannover, Germany.
Rita
R
Gerardy-Schahn
Max-Planck-Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany glyXera GmbH, Magdeburg, Germany.
Erdmann
E
Rapp
Unit of Infection Models, German Primate Center, Göttingen, Germany.
Christiane
C
Stahl-Hennig
Infection Biology Unit, German Primate Center, Göttingen, Germany spoehlmann@dpz.eu.
Stefan
http://orcid.org/0000-0001-6086-9136
S
Pöhlmann
D000818:Animals; D002460:Cell Line; D015686:Gene Products, env / Q000378:metabolism*; D015658:HIV Infections / Q000635:transmission / Q000821:virology; D015497:HIV-1 / Q000378:metabolism / Q000472:pathogenicity; D006801:Humans; D008253:Macaca mulatta; D008358:Mannose / Q000378:metabolism; D009092:Mucous Membrane / Q000821:virology*; D011134:Polysaccharides / Q000378:metabolism*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000635:transmission* / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000378:metabolism / Q000472:pathogenicity*
D015686:Gene Products, env; D011134:Polysaccharides; D008358:Mannose