Analysis of the Fungicide Boscalid in Horticultural Crops Using an Enzyme-Linked Immunosorbent Assay and an Immunosensor Based on Surface Plasmon Resonance.
A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) and an immunosensor based on surface plasmon resonance (SPR-sensor) were developed for fungicide boscalid determination in horticultural crops. To produce antiboscalid monoclonal antibodies (MoAb BSC7 and MoAb BSC72) for these assays, a hapten of boscalid was synthesized and conjugated to keyhole limpet hemocyanin for Balb/c mouse immunization. The working range of the dc-ELISA was 0.8-16 ng/mL with MoAb BSC7 and 2.5-120 ng/mL with MoAb BSC72, and that of the SPR-sensor was 17-80 ng/mL with MoAb BSC7. The dc-ELISA and SPR-sensor were compared for their sensitivity in determining boscalid residues at the maximum residue limit of 1-40 mg/kg for horticultural crops in Japan. Recovery of the spiked boscalid was 85-109% by the SPR-sensor and 100-124% by the dc-ELISA. On real tomato samples, the results obtained by both of these immunoassays correlated well with the results obtained by high-performance liquid chromatography.
Advanced Scientific Technology & Management Research Institute of Kyoto , Shimogyo-ku, Kyoto 600-8813, Japan.
Yuki
Y
Hirakawa
Advanced Scientific Technology & Management Research Institute of Kyoto , Shimogyo-ku, Kyoto 600-8813, Japan.
Tomomi
T
Yamasaki
Toyohashi University of Technology , Toyohashi, Aichi 441-8580, Japan.
Ayako
A
Harada
Aichi Agricultural Research Center , Nagakute, Aichi 480-1193, Japan.
Toshiya
T
Ohtake
Aichi Science & Technology Foundation , Toyota, Aichi 470-0356, Japan.
Kayo
K
Adachi
Toyohashi University of Technology , Toyohashi, Aichi 441-8580, Japan.
Seiji
S
Iwasa
Kyoto Women's University , Higashiyama-ku, Kyoto 605-8501, Japan.
Hiroshi
H
Narita
Advanced Scientific Technology & Management Research Institute of Kyoto , Shimogyo-ku, Kyoto 600-8813, Japan.|Research & Development Division, Horiba, Ltd. , Minami-ku, Kyoto 601-8510, Japan.
Shiro
S
Miyake
D000818:Animals; D001713:Biphenyl Compounds / Q000032:analysis*; D018556:Crops, Agricultural / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D005659:Fungicides, Industrial / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D009536:Niacinamide / Q000031:analogs & derivatives* / Q000032:analysis; D020349:Surface Plasmon Resonance / Q000379:methods*
D001713:Biphenyl Compounds; D005659:Fungicides, Industrial; D009536:Niacinamide; C550088:2-chloro-N-(4-chlorobiphenyl-2-yl)nicotinamide
ELISA; pesticide; surface plasmon resonance