A comparative study for the inactivation of multidrug resistance bacteria using dielectric barrier discharge and nano-second pulsed plasma.
Bacteria can be inactivated through various physical and chemical means, and these have always been the focus of extensive research. To further improve the methodology for these ends, two types of plasma systems were investigated: nano-second pulsed plasma (NPP) as liquid discharge plasma and an Argon gas-feeding dielectric barrier discharge (Ar-DBD) as a form of surface plasma. To understand the sterilizing action of these two different plasma sources, we performed experiments with Staphylococcus aureus (S. aureus) bacteria (wild type) and multidrug resistant bacteria (Penicillum-resistant, Methicillin-resistant and Gentamicin-resistant). We observed that both plasma sources can inactivate both the wild type and multidrug-resistant bacteria to a good extent. Moreover, we observed a change in the surface morphology, gene expression and β-lactamase activity. Furthermore, we used X-ray photoelectron spectroscopy to investigate the variation in functional groups (C-H/C-C, C-OH and C=O) of the peptidoglycan (PG) resulting from exposure to plasma species. To obtain atomic scale insight in the plasma-cell interactions and support our experimental observations, we have performed molecular dynamics simulations to study the effects of plasma species, such as OH, H2O2, O, O3, as well as O2 and H2O, on the dissociation/formation of above mentioned functional groups in PG.
Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul, Korea 139-701.
Ji Hoon
JH
Park
Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul, Korea 139-701.
Naresh
N
Kumar
Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul, Korea 139-701.
Dae Hoon
DH
Park
Research Group PLASMANT, Department of Chemistry, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium.
Maksudbek
M
Yusupov
Research Group PLASMANT, Department of Chemistry, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium.
Erik C
EC
Neyts
Research Group PLASMANT, Department of Chemistry, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium.
Christof C W
CC
Verlackt
Research Group PLASMANT, Department of Chemistry, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium.
Annemie
A
Bogaerts
Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul, Korea 139-701.
Min Ho
MH
Kang
Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul, Korea 139-701.
Han Sup
HS
Uhm
Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul, Korea 139-701.
Eun Ha
EH
Choi
Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul, Korea 139-701.
Pankaj
P
Attri
D004203:Disinfection / Q000379:methods*; D024901:Drug Resistance, Multiple, Bacterial; D015964:Gene Expression Regulation, Bacterial; D050296:Microbial Viability; D010457:Peptidoglycan / Q000737:chemistry / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
D010457:Peptidoglycan; D017382:Reactive Oxygen Species; D001618:beta-Lactamases