Malathion Resistance Status and Mutations in Acetylcholinesterase Gene (Ace) in Japanese Encephalitis and Filariasis Vectors from Endemic Area in India.
Japanese encephalitis (JE) and lymphatic filariasis (LF) are endemic in estern part of Uttar Pradesh in India and transmitted by Culex mosquitoes (Diptera: Culicidae). JE vaccination and mass drug administration for JE and LF management is being undertaken respectively. In addition to this, indoor residual spraying and fogging are used for the control of mosquito vectors. Organophosphate resistance in mosquito is dependent on alteration in acetylcholinesterase (Ace) gene. Hence, it is important to evaluate organophosphate resistance in Culex tritaeniorhynchus Giles (JE vector) and Culex quinquefasciatus Say (LF vector). The current study showed the presence of resistant populations and F331W mutation in Cx. tritaeniorhynchus and G119S mutation in Cx. quinquefasciatus insensitive Ace genes. Resistant populations of these two vectors increase the chances of spreading of resistance in the natural population and may cause failure of intervention programs that include organophosphates against these two vectors in future.
National Institute of Virology, GKP Unit, B.R.D. Medical College Campus, Gorakhpur 273013, India.
Brij Ranjan
BR
Misra
National Institute of Virology, GKP Unit, B.R.D. Medical College Campus, Gorakhpur 273013, India. gore.milind@gmail.com.
Milind
M
Gore
D000110:Acetylcholinesterase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D003465:Culex / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D004672:Encephalitis, Japanese / Q000635:transmission / Q000821:virology; D005260:Female; D005368:Filariasis / Q000469:parasitology / Q000635:transmission; D007194:India; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007303:Insect Vectors / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D008294:Malathion / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009154:Mutation; D017422:Sequence Analysis, DNA
D019476:Insect Proteins; D007306:Insecticides; D000110:Acetylcholinesterase; D008294:Malathion
Culex quinquefasciatus; Culex tritaeniorhynchus; acetylcholinesterase; insecticide bioassay