Engineering of Biocompatible pH-Responsive Nanovehicles from Acetalated Cyclodextrins as Effective Delivery Systems for Tumor Therapy.
There is still an unmet demand for materials with excellent biocompatibility, controlled hydrolytic capability, and elegant responsiveness to chemical or physical stimuli. To engineer biocompatible materials from β-cyclodextrin (β-CD), in this study, we synthesized acetalated β-CDs (Ac-βCDs) by one-pot acetalation using 2-ethoxypropene as an acetonation reagent, which can be further processed into nanoparticles (NPs) via the emulsion technique. Ac-βCD NPs showed pH-labile hydrolysis and pH-triggered release of docetaxel (DTX) payload. Both properties were mainly dominated by the molar ratio of linear to cyclic acetal, which can be conveniently modulated by the acetalation time used for materials synthesis. Ac-βCD NPs were found to be biocompatible in both in vitro cell culture and in vivo acute toxicity evaluations following intravenous injection. In vitro cell culture experiments demonstrated that antitumor activity of DTX against both sensitive and resistant cancer cells was remarkably improved by formulation into Ac-βCD nanomedicines. In vivo antitumor study also substantiated the dramatically enhanced efficacy of DTX/Ac-βCD NPs in a melanoma-bearing nude mouse model. These studies demonstrated that NPs derived from Ac-βCDs may serve as biocompatible and effective carriers for drug delivery.
Dinglin
D
Zhang
Yanling
Y
Wei
Kai
K
Chen
Hao
H
Gong
Songling
S
Han
Jiawei
J
Guo
Xiaohui
X
Li
Jianxiang
J
Zhang
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D002478:Cells, Cultured; D000077143:Docetaxel; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D043823:Taxoids / Q000008:administration & dosage* / Q000493:pharmacokinetics; D047392:beta-Cyclodextrins / Q000737:chemistry* / Q000493:pharmacokinetics
D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D004337:Drug Carriers; D011108:Polymers; D043823:Taxoids; C018801:acetal copolymer; D047392:beta-Cyclodextrins; D000077143:Docetaxel