[Time bacterial growth in blood cultures in neonates].
BACKGROUND
Sepsis is a major cause of neonatal morbidity and mortality.


OBJECTIVE
To detect the time when the bacterial growth curve is evidenced in the blood sample inoculated blood cultures and comparing the times of bacterial growth between Gram negative and Gram positive bacteria, among the types of neonatal sepsis and identifying microorganisms more often isolated from preterm and term.


METHODS
A descriptive study. 114 positive blood cultures from 1,932 blood cultures taken from 01-May-2010 and 31-May-2014 were evaluated. Data were analyzed with Stata(®) 11.0.


RESULTS
5.9% of blood cultures had bacterial growth. The median and interquartile range of Gram negative times of bacterial growth was 11h (10-13h), for Gram positive coagulase-negative Staphylococcus different (CoNS) 12h (12-18h) and CoNS 42h (36-44h). 95.8% of Gram positive and 96% of Gram negative, were the times of bacterial growth≤24h incubation, whereas the 100% CoNS was positive≤62h of incubation.


CONCLUSIONS
100% of sepsis by Gram negative and Gram positive no CoNS and 90% of those caused by CoNS are identified in blood cultures in 48h, so we can conclude that to rule out sepsis, an incubation period of 48h in blood cultures is sufficient.
Fundación Hospital San José de Buga, Buga, Colombia. Electronic address: lamendozat@gmail.com.
Luis
L
Mendoza
Universidad del Valle, Cali, Colombia.
Miguel
M
Osorio
Laboratorio Ángel, Sede Fundación Hospital San José de Buga, Buga, Colombia.
Marisol
M
Fernández
Fundación Hospital San José de Buga, Buga, Colombia.
Claudia
C
Henao
Fundación Hospital San José de Buga, Buga, Colombia.
Martha
M
Arias
Facultad Ciencias de la Salud, Unidad Central del Valle, Tuluá, Colombia.
Laura
L
Mendoza
Facultad Ciencias de la Salud, Unidad Central del Valle, Tuluá, Colombia.
Stefania
S
Manzano
Facultad Ciencias de la Salud, Unidad Central del Valle, Tuluá, Colombia.
Ana
A
Varela
D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D000071997:Blood Culture; D005260:Female; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D006094:Gram-Positive Bacteria / Q000302:isolation & purification*; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D018805:Sepsis / Q000175:diagnosis* / Q000382:microbiology; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology; D013210:Staphylococcus / Q000201:enzymology / Q000302:isolation & purification; D013997:Time Factors
Blood culture; Coagulase-negative Staphylococcus; Hemocultivo; Sepsis; Staphylococcus Coagulasa negativo; Tiempo de crecimiento bacteriano; Time bacterial growth