Acclimation of Chlamydomonas reinhardtii to ultraviolet radiation and its impact on chemical toxicity.
The toxicity of chemical pollutants can be modulated under stressful environmental conditions, such as increased temperature, salinity or ultraviolet radiation (UVR), due to the interaction of effects during simultaneous stressor exposure. However, organisms may acclimate to such conditions by activation of physiological and biochemical defence mechanisms. In sequential exposures, organisms acclimated to environmental stressors may display an increased sensitivity or co-tolerance towards chemical pollutants. It has been suggested that co-tolerance might be expected for similarly acting stressors due to common defence mechanisms. To test this for combinations of UVR and chemical stressors, we first acclimatized the model green alga Chlamydomonas reinhardtii to UVR and subsequently compared the sensitivity of UVR pre-exposed and control algae towards chemicals. Selected chemicals all act on photosynthesis and thus share a common physiological target, but display distinct toxicity mechanisms. Results showed that UVR pre-exposure for four days partially inhibited algal growth and photosynthesis, but also increased algal tolerance to higher UVR levels, confirming UVR acclimation. HPLC analysis of algal pigments indicated that UVR acclimation might in part be explained by the protective function of lutein while the contribution of UVR absorbing compounds was less clear. Challenge exposure to chemicals in the absence of UVR showed that acclimated algae were co-tolerant to the photosensitizer rose bengal, but not to the herbicides paraquat and diuron, suggesting that the fast physiological and biochemical defence mechanisms that conferred tolerance of algae towards higher UVR levels were related to singlet oxygen defence. The presented study suggests that knowledge of the molecular toxicity mechanisms of chemicals, rather than their general physiological target, is needed in order to predict co-tolerance between environmental and chemical stressors.
Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Environmental Toxicology, 8600 Duebendorf, Switzerland; ETH Zürich, Institute of Biogeochemistry and Pollutant Dynamics, 8092 Zürich, Switzerland.
Muris
M
Korkaric
Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Environmental Toxicology, 8600 Duebendorf, Switzerland; ETH Zürich, Institute of Biogeochemistry and Pollutant Dynamics, 8092 Zürich, Switzerland.
Mao
M
Xiao
Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Environmental Toxicology, 8600 Duebendorf, Switzerland.
Renata
R
Behra
Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Environmental Toxicology, 8600 Duebendorf, Switzerland; ETH Zürich, Institute of Biogeochemistry and Pollutant Dynamics, 8092 Zürich, Switzerland. Electronic address: rik.eggen@eawag.ch.
Rik I L
RI
Eggen
D000064:Acclimatization; D016825:Chlamydomonas reinhardtii / Q000187:drug effects* / Q000528:radiation effects*; D004237:Diuron / Q000633:toxicity*; D006540:Herbicides / Q000633:toxicity; D010269:Paraquat / Q000633:toxicity; D010788:Photosynthesis / Q000187:drug effects; D013312:Stress, Physiological / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays; D014874:Water Pollutants, Chemical / Q000633:toxicity; D014875:Water Pollutants, Radioactive / Q000633:toxicity*
D006540:Herbicides; D014874:Water Pollutants, Chemical; D014875:Water Pollutants, Radioactive; D004237:Diuron; D010269:Paraquat
Acclimation; Chlamydomonas reinhardtii; Co-tolerance; Multiple stressor effects; Ultraviolet radiation