Integrative proteome analysis of Brachypodium distachyon roots and leaves reveals a synergetic responsive network under H2O2 stress.
The plant oxidative stress response is vital for defense against various abiotic and biotic stresses. In this study, ultrastructural changes and the proteomic response to H2O2 stress in roots and leaves of the model plant Brachypodium distachyon were studied. Transmission electron microscopy (TEM) showed that the ultrastructural damage in roots was more serious than in leaves. Particularly, the ultrastructures of organelles and the nucleus in root tip cells were damaged, leading to the inhibition of normal biological activities of roots, which then spread throughout the plant. Based on two-dimensional electrophoresis (2-DE) and MALDI-TOF/TOF-MS, 84 and 53 differentially accumulated protein (DAP) spots representing 75 and 45 unique proteins responsive to H2O2 stress in roots and leaves, respectively, were identified. These protein species were mainly involved in signal transduction, energy metabolism, redox homeostasis/stress defense, protein folding/degradation, and cell wall/cell structure. Interestingly, two 14-3-3 proteins (GF14-B and GF14-D) were identified as DAPs in both roots and leaves. Protein-protein interaction (PPI) analysis revealed a synergetic H2O2-responsive network.
College of Life Science, Capital Normal University, 100048 Beijing, China.
Yan-Wei
YW
Bian
College of Life Science, Capital Normal University, 100048 Beijing, China; VCU Philips Institute for Oral Health Research, Virginia Commonwealth University, School of Dentistry, Department of Oral and Craniofacial Molecular Biology, 23298 Richmond, VA, USA.
Dong-Wen
DW
Lv
College of Life Science, Capital Normal University, 100048 Beijing, China.
Zhi-Wei
ZW
Cheng
College of Life Science, Capital Normal University, 100048 Beijing, China.
Ai-Qin
AQ
Gu
College of Life Science, Capital Normal University, 100048 Beijing, China.
Hui
H
Cao
College of Life Science, Capital Normal University, 100048 Beijing, China. Electronic address: yanym@cnu.edu.cn.
Yue-Ming
YM
Yan
D058431:Brachypodium / Q000187:drug effects / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology; D006861:Hydrogen Peroxide / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013312:Stress, Physiological / Q000187:drug effects / Q000502:physiology*; D018511:Systems Integration
D010940:Plant Proteins; D020543:Proteome; D006861:Hydrogen Peroxide
14–3–3 proteins; Brachypodium distachyon; H(2)O(2) stress; Leaf; Proteome; Root