Atlantic cod (Gadus morhua) larvae can biosynthesis phospholipid de novo from 2-oleoyl-glycerol and glycerol precursors.
The dietary requirement of phospholipid (PL) of fish larvae has been suggested to originate in an inefficient ability for de novo biosynthesis of PL based on dietary triacylglycerol (TAG). The main objective of the present study was to investigate whether cod larvae could synthesis PL from sn-2-monoacylglycerol (2-MAG) and glycerol precursors. A tube feeding method was used to deliver equal molar aliquots of 2-oleoyl-[1,2,3-(3)H]glycerol and [U-(14)C] glycerol together with bovine serum albumin (BSA) bound 16:0 (palmitic acid) and 22:6n-3 (docosahexaenoic acid, DHA), with or without choline chloride to the foregut of anesthetized cod larvae and thereafter monitoring the metabolism of these components in the larvae through 4 h following injection. Our results showed that both 2-MAG and glycerol precursors contributed to the de novo synthesis of phosphatidylcholine (PC) and the 2-MAG pathway predominated over the G-3-P (glycerol-3-phosphate) pathway in the synthesis of TAG and PC. The molecular ratio of PC/TAG obtained from the 2-MAG and the G-3-P pathways was 0.44-0.74 and 1.02-2.06 within the first hour of tube feeding, suggesting they might have comparable biosynthesis ability of PC and TAG under the conditions of the present study. Furthermore, supplementation of choline chloride significantly increased PC/TAG ratio (p < 0.05) for both pathways. However, further studies are needed to quantify the enzyme activity involved in the CDP-choline (cytidine diphosphate choline) pathway, and the function of choline either in simulating PC synthesis or TAG catabolism or both needs further investigation.
Department of Biology, Norwegian University of Science and Technology, NO-7491, Trondheim, Norway. lks00@163.com.
Keshuai
K
Li
Department of Biology, Norwegian University of Science and Technology, NO-7491, Trondheim, Norway.
Rolf Erik
RE
Olsen
Department of Biology, Norwegian University of Science and Technology, NO-7491, Trondheim, Norway.
Mari-Ann
MA
Østensen
BioTrix, NO-7022, Trondheim, Norway.
Dag
D
Altin
Department of Biology, Norwegian University of Science and Technology, NO-7491, Trondheim, Norway.
Elin
E
Kjørsvik
Department of Biology, Norwegian University of Science and Technology, NO-7491, Trondheim, Norway.
Yngvar
Y
Olsen
D000818:Animals; D002794:Choline / Q000494:pharmacology; D048389:Gadus morhua / Q000378:metabolism; D005990:Glycerol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects* / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis*; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D010743:Phospholipids; D012710:Serum Albumin, Bovine; D002794:Choline; D005990:Glycerol
Atlantic cod larvae; Phospholipid; Tube feeding; de novo synthesis