Stella controls chromocenter formation through regulation of Daxx expression in 2-cell embryos.
In mammals, the structure of the pericentromeric region alters from a ring structure to a dot-like structure during the 2-cell stage. This structural alteration is termed chromocenter formation (CF) and is required for preimplantation development. Although reverse transcripts of major satellite repeats at pericentromeric regions are known to play roles in CF, its underlying mechanism is not fully understood. We previously reported that Stella (also known as PGC7 and Dppa3) deficiency led to developmental arrest at the preimplantation stage, accompanied by frequent chromosome segregation. In this study, we further investigated the effect of Stella deficiency on chromatin reorganization. The Stella-null embryos exhibited impaired CF and reduced expression of the reverse strand of major satellite repeats. In addition, the accumulation of H3.3, a histone H3 variant associated with transcriptional activation, at the pericentromeric regions and expression of the H3.3-specific chaperone Daxx were reduced in Stella-null embryos. These abnormalities were restored by the enforced expression of Daxx in Stella-null embryos. Thus, Stella controls the expression of Daxx and ensures chromatin reorganization in early embryos.
Graduate School of Frontier Biosciences, Osaka University, Osaka 565-0871, Japan.
Tatsuhiko
T
Arakawa
Department of Pathology, Osaka University, Osaka 565-0871, Japan; Japan Science and Technology Agency, Core Research for Evolutional Science and Technology, Saitama 332-0012, Japan. Electronic address: nakatani@patho.med.osaka-u.ac.jp.
Tsunetoshi
T
Nakatani
Graduate School of Frontier Biosciences, Osaka University, Osaka 565-0871, Japan; Department of Pathology, Osaka University, Osaka 565-0871, Japan.
Masaaki
M
Oda
Department of Pathology, Osaka University, Osaka 565-0871, Japan; Department of Neurology, Medical School, Osaka University, Osaka 565-0871, Japan.
Yasuyoshi
Y
Kimura
Department of Pathology, Osaka University, Osaka 565-0871, Japan.
Yoichi
Y
Sekita
Graduate School of Frontier Biosciences, Osaka University, Osaka 565-0871, Japan; Department of Pathology, Osaka University, Osaka 565-0871, Japan.
Tohru
T
Kimura
Japan Science and Technology Agency, Core Research for Evolutional Science and Technology, Saitama 332-0012, Japan; Nagahama Institute of Bio-Science and Technology, Shiga 526-0829, Japan.
Toshinobu
T
Nakamura
Graduate School of Frontier Biosciences, Osaka University, Osaka 565-0871, Japan; Department of Pathology, Osaka University, Osaka 565-0871, Japan; Japan Science and Technology Agency, Core Research for Evolutional Science and Technology, Saitama 332-0012, Japan. Electronic address: tnakano@patho.med.osaka-u.ac.jp.
Toru
T
Nakano
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D002868:Chromosomal Proteins, Non-Histone; D020090:Chromosome Segregation; D056970:Co-Repressor Proteins; D005260:Female; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D006570:Heterochromatin / Q000378:metabolism* / Q000648:ultrastructure; D006657:Histones / Q000378:metabolism / Q000648:ultrastructure; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D018832:Molecular Chaperones; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D012097:Repressor Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D015053:Zygote / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure
D002352:Carrier Proteins; D002868:Chromosomal Proteins, Non-Histone; D056970:Co-Repressor Proteins; C491473:Daxx protein, mouse; C462180:Dppa3 protein, mouse; D006570:Heterochromatin; D006657:Histones; D047908:Intracellular Signaling Peptides and Proteins; D018832:Molecular Chaperones; D009687:Nuclear Proteins; D012097:Repressor Proteins
Chromocenter formation; H3.3; Histone chaperone; Major satellite; Zygote