Internal standard method for the measurement of doxorubicin and daunorubicin by capillary electrophoresis with in-column double optical-fiber LED-induced fluorescence detection.
An internal standard method has been developed for the simultaneous determination of anthracycline antibiotics, doxorubicin (DOX) and daunorubicin (DAN), in rabbit plasma using capillary electrophoresis (CE) with in-column double optical-fiber LED-induced fluorescence detection (CE-ICDOF-LED-FLD). Rhodamine B (RhB) was selected as an internal standard because its emission wavelength is similar to that of the anthracycline antibiotics. Parameters including buffer pH, buffer concentration, organic solvents and separation voltage have been investigated to explore the sensitivity and separation efficiency of DOX and DAN. The optimal electrophoretic separation conditions were a borate buffer (15 mM, pH 9.0) containing 50% acetonitrile (v/v), 10 s hydrodynamic injection at a height of 20 cm and a separation voltage of 15 kV. The developed CE-ICDOF-LED-FLD method provides limits of detection of 18 and 13 ng/mL for DOX and DAN in rabbit plasma samples, respectively. The recoveries ranging from 93.7 to 104.8% and the relative standard deviations at 1.1-1.7% were achieved for DOX and DAN in spiked rabbit plasma samples.
College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong 637000, PR China. Electronic address: xiupeiyang@163.com.
Xiupei
X
Yang
College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong 637000, PR China.
Huanhuan
H
Gao
College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong 637000, PR China.
Fan
F
Qian
College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong 637000, PR China.
Chuan
C
Zhao
Exposure and Biomonitoring Division, Health Canada, 50Colombine Driveway, Ottawa K1A 0K9, Canada. Electronic address: xiangjun.liao@mail.mcgill.ca.
Xiangjun
X
Liao
D000818:Animals; D002626:Chemistry, Pharmaceutical / Q000379:methods / Q000592:standards*; D003630:Daunorubicin / Q000097:blood*; D004317:Doxorubicin / Q000097:blood*; D019075:Electrophoresis, Capillary / Q000379:methods / Q000592:standards; D055100:Optical Fibers / Q000592:standards*; D011817:Rabbits; D012015:Reference Standards; D013050:Spectrometry, Fluorescence / Q000379:methods / Q000592:standards
D004317:Doxorubicin; D003630:Daunorubicin
Capillary electrophoresis; Daunorubicin; Doxorubicin; LED-induced fluorescence detection