Measurement of creatinine in human plasma using a functional porous polymer structure sensing motif.
In this study, a new method for detecting creatinine was developed. This novel sensor comprised of two ionic liquids, poly-lactic-co-glycolic acid (PLGA) and 1-butyl-3-methylimidazolium (BMIM) chloride, in the presence of 2',7'-dichlorofluorescein diacetate (DCFH-DA). PLGA and BMIM chloride formed a functional porous polymer structure (FPPS)-like structure. Creatinine within the FPPS rapidly hydrolyzed and released OH(-), which in turn converted DCFH-DA to DCFH, developing an intense green color or green fluorescence. The conversion of DCFH to DCF(+) resulted in swelling of FPPS and increased solubility. This DCF(+)-based sensor could detect creatinine levels with detection limit of 5 µM and also measure the creatinine in blood. This novel method could be used in diagnostic applications for monitoring individuals with renal dysfunction.
Department of Bionanotechnology, Gachon Medical Research Institute, Gachon University, Seongnam, South Korea.
Sitansu Sekhar
SS
Nanda
Department of Bionanotechnology, Gachon Medical Research Institute, Gachon University, Seongnam, South Korea.
Seong Soo A
SS
An
Department of Chemistry, Myongji University, Yongin, South Korea ; Department of Environmental Engineering, Myongji University, Yongin, South Korea.
Dong Kee
DK
Yi
D015374:Biosensing Techniques / Q000379:methods*; D003404:Creatinine / Q000097:blood*; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007093:Imidazoles / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
C525963:1-butyl-3-methylimidazolium; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007093:Imidazoles; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C029569:diacetyldichlorofluorescein; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003404:Creatinine
FPPS; PLGA; blood; creatinine; diagnosis