Edaravone attenuates monocyte adhesion to endothelial cells induced by oxidized low-density lipoprotein.
Oxidized low-density lipoprotein (oxLDL) plays a vital role in recruitment of monocytes to endothelial cells, which is important during early stages of atherosclerosis development. Edaravone, a potent and novel scavenger of free radicals inhibiting hydroxyl radicals, has been clinically used to reduce the neuronal damage following ischemic stroke. In the present study, Edaravone was revealed to markedly reduce oxLDL-induced monocyte adhesion to human umbilical vein endothelial cells (HUVECs). The inhibitory mechanism of Edaravone was associated with suppression of the chemokine MCP-1 and adhesion molecule VCAM-1 and ICAM-1 expression. In addition, luciferase reporter assay results revealed that administration of Edaravone attenuated the increase in NF-κB transcriptional activity induced by oxLDL. Notably, it's also shown that Edaravone treatment blocked oxLDL induced p65 nuclear translocation in HUVECs. Results indicate that Edaravone negatively regulates endothelial inflammation.
Department of Cardiology, First Affiliated Hospital of Henan University of Science and Technology, China. Electronic address: zjlee038@163.com.
Zhijuan
Z
Li
Department of Cardiology, First Affiliated Hospital of Henan University of Science and Technology, China.
Jianxin
J
Cheng
Department of Cardiology, First Affiliated Hospital of Henan University of Science and Technology, China.
Liping
L
Wang
D000983:Antipyrine / Q000031:analogs & derivatives* / Q000494:pharmacology; D050197:Atherosclerosis / Q000188:drug therapy / Q000517:prevention & control; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D000077553:Edaravone; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D000077553:Edaravone; D000983:Antipyrine
Edaravone; Monocytes adhesion; NF-κB; Oxidized low-density lipoprotein (oxLDL); Vascular inflammation