Identification of compounds from high-fat and extra virgin olive oil-supplemented diets in whole mouse liver extracts and isolated mitochondria using mass spectrometry.
Nonalcoholic steatohepatitis (NASH) is a fatty liver disorder that could be improved with extra virgin olive oil (EVOO) supplementation in diet. We propose the monitoring, in whole mouse liver extracts and in isolated mitochondria, of the absorption of compounds from three different diets: standard (CT), high-fat (HFD) and high-fat supplemented with EVOO (HFSO). Male mice were submitted to one of the following three diets: CT or HFD for 16 weeks or HFD for 8 weeks followed by additional 8 weeks with HFSO. Following this period, liver was extracted for histological evaluation, mitochondria isolation and mass spectrometry analyses. Diets, liver extracts and Percoll-purified mitochondria were analyzed using ESI-MS and the lipidomics approach. Morphological, histological and spectrometric results indicated a decrease in NASH severity with EVOO supplementation in comparison with animals maintained with HFD. Spectrometric data also demonstrated that some compounds presented on the diets are absorbed by the mitochondria. EVOO was shown to be a potential therapeutic alternative in food for NASH. Our results are in accordance with the proposition that the major factor that influences different responses to diets is their composition - and not only calories - especially when it comes to studies on obesity.
INNOVARE Biomarkers Laboratory, School of Medical Sciences, University of Campinas, Campinas, SP, Brazil.
Gustavo Aparecido
GA
dos Santos
INNOVARE Biomarkers Laboratory, School of Medical Sciences, University of Campinas, Campinas, SP, Brazil.
Mônica Siqueira
MS
Ferreira
INNOVARE Biomarkers Laboratory, School of Medical Sciences, University of Campinas, Campinas, SP, Brazil.
Diogo Noin
DN
de Oliveira
Sport Sciences Course, Faculty of Applied Sciences, University of Campinas, Campinas, SP, Brazil.
Vanessa
V
de Oliveira
Experimental Neurodegeneration Laboratory, School of Medical Sciences, University of Campinas, Campinas, SP, Brazil.
Edilene S
ES
Siqueira-Santos
Sport Sciences Course, Faculty of Applied Sciences, University of Campinas, Campinas, SP, Brazil.
Dennys Esper Corrêa
DE
Cintra
Experimental Neurodegeneration Laboratory, School of Medical Sciences, University of Campinas, Campinas, SP, Brazil.
Roger Frigério
RF
Castilho
Laboratory of Cell Signaling, School of Medical Sciences, University of Campinas, Campinas, SP, Brazil.
Lício Augusto
LA
Velloso
INNOVARE Biomarkers Laboratory, School of Medical Sciences, University of Campinas, Campinas, SP, Brazil.
Rodrigo Ramos
RR
Catharino
D000818:Animals; D059305:Diet, High-Fat; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008110:Liver Extracts / Q000737:chemistry*; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000737:chemistry*; D065626:Non-alcoholic Fatty Liver Disease / Q000378:metabolism; D009765:Obesity; D000069463:Olive Oil / Q000378:metabolism* / Q000494:pharmacology; D025341:Principal Component Analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D008110:Liver Extracts; D000069463:Olive Oil
Omics; diet; electrospray ionization mass spectrometry; extra virgin olive oil; nonalcoholic steatohepatitis