The Effects of Probiotic Soymilk Fortified with Omega-3 on Blood Glucose, Lipid Profile, Haematological and Oxidative Stress, and Inflammatory Parameters in Streptozotocin Nicotinamide-Induced Diabetic Rats.
OBJECTIVE
The aim of the present study was to evaluate the effects of probiotic soymilk fortified with omega-3 in diabetic rats.


METHODS
Soymilk (SM), fermented soymilk (FSM), and fermented soymilk fortified with omega-3 (FSM + omega-3) were prepared. Rats were randomly assigned to five groups of 13 animals per group. Diabetes was induced by a single injection of streptozotocin (STZ) 15 min after the intraperitoneal administration of nicotinamide (NA). Normal control (NC) and diabetic control (DC) rats received 1 mL/day of distilled water and three groups of diabetic rats were given 1 mL/day of SM, FSM, and FSM + omega-3 products by oral gavage for 28 days.


RESULTS
Three products significantly (P < 0.05) reduced blood glucose, total cholesterol (TC), triglyceride (TG), and malondialdehyde (MDA) concentrations compared to the DC group, with the maximum reduction seen in the FSM + omega-3 group. Body weight, red blood cells (RBC), haemoglobin (Hb), haematocrit, and superoxide dismutase (SOD) also significantly increased in the FSM + omega-3 group. In the FSM + omega-3 group, MDA level compared with the SM and FSM groups and high sensitivity C-reactive protein (hs-CRP) concentrations compared with the DC and FSM groups were significantly lower (P < 0.05).


CONCLUSIONS
Fermented soymilk fortified with omega-3 may be beneficial in diabetes.
Student Research Committee, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz 7153675541, Iran.
Mohsen
M
Mohammadi Sartang
Department of Food Hygiene and Quality Control, Nutrition and Food Sciences Research Center, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz 7153675541, Iran.
Seyed Mohammad
SM
Mazloomi
Stem Cell and Transgenic Technology Research Center, Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz 7134874478, Iran.
Nader
N
Tanideh
Research Center for Health Sciences, Department of Epidemiology, School of Health, Shiraz University of Medical Sciences, Shiraz 7153675541, Iran.
Abbas
A
Rezaian Zadeh
D000818:Animals; D001786:Blood Glucose / Q000737:chemistry*; D001835:Body Weight; D002097:C-Reactive Protein / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000178:diet therapy*; D004912:Erythrocytes / Q000166:cytology; D015525:Fatty Acids, Omega-3 / Q000737:chemistry*; D005285:Fermentation; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D007249:Inflammation; D008055:Lipids / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009536:Niacinamide / Q000737:chemistry; D018384:Oxidative Stress; D019936:Probiotics / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D041942:Soy Milk; D013311:Streptozocin / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D015525:Fatty Acids, Omega-3; D006454:Hemoglobins; D008055:Lipids; D014280:Triglycerides; D009536:Niacinamide; D008315:Malondialdehyde; D013311:Streptozocin; D002097:C-Reactive Protein; D002784:Cholesterol; D013482:Superoxide Dismutase