Divinyl chlorophyll a in the marine eukaryotic protist Alexandrium ostenfeldii (Dinophyceae).
Here it is reported the first detection of DV-chl a together with the usual chl a in the marine dinoflagellate Alexandrium ostenfeldii from the Baltic Sea. Growth response and photosynthetic parameters were examined at two irradiances (80 and 240 μmol photons m(-2) s(-1)) and temperatures (15 °C and 19 °C) in a divinylic strain (AOTV-OS20) versus a monovinylic one (AOTV-OS16), using in vivo chl a fluorescence kinetics of PSII to characterize photosynthetic parameters by pulse amplitude modulated fluorescence, (14)C assimilation rates and toxin analyses. The divinylic isolate exhibited slower growth and stronger sensitivity to high irradiance than normal chl a strain. DV-chl a : chl a ratios decreased along time (from 11.3 to < 0.5 after 10 months) and to restore them sub-cloning and selection of strains with highest DV-chl a content was required. A mutation and/or epigenetic changes in the expression of divinyl reductase gene/s in A. ostenfeldii may explain this altered pigment composition. Despite quite severe limitations (reduced fitness and gradual loss of DV-chl a content), the DV-chl a-containing line in A. ostenfeldii could provide a model organism in photosynthetic studies related with chl biosynthesis and evolution.
Instituto Español de Oceanografía (IEO), Centro Oceanográfico de Vigo, Spain.
Francisco
F
Rodríguez
Instituto de Investigaciones Marinas (CSIC), Vigo, Spain.
José Luis
JL
Garrido
Departamento de Ecología y Biología Animal, Universidad de Vigo, Spain.
Cristina
C
Sobrino
Trondhjem Biological Station, Norwegian University of Technology and Science (NTNU), Trondheim, Norway.
Geir
G
Johnsen
Instituto de Investigaciones Marinas (CSIC), Vigo, Spain.
Pilar
P
Riobó
Instituto de Investigaciones Marinas (CSIC), Vigo, Spain.
José
J
Franco
Trondhjem Biological Station, Norwegian University of Technology and Science (NTNU), Trondheim, Norway.
Inga
I
Aamot
Instituto Español de Oceanografía (IEO), Centro Oceanográfico de Vigo, Spain.
Isabel
I
Ramilo
Instituto de Investigaciones Marinas (CSIC), Vigo, Spain.
Noelia
N
Sanz
Marine Research Centre, Finnish Environment Institute, Helsinki, Finland.
Anke
A
Kremp
D002070:Butadienes / Q000737:chemistry; D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D004141:Dinoflagellida / Q000254:growth & development* / Q000378:metabolism*; D005453:Fluorescence; D009792:Oceans and Seas; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology*; D013696:Temperature; D014753:Vinyl Compounds / Q000378:metabolism*
D002070:Butadienes; D014753:Vinyl Compounds; C510432:divinyl chlorophyll a; D002734:Chlorophyll; D010088:Oxidoreductases; C031763:1,3-butadiene; D000077194:Chlorophyll A