Fluorescence polarization measurement system using a liquid crystal layer and an image sensor.
The detection system which enables simultaneous fluorescence polarization (FP) measurement of multiple samples was proposed and proven by a proof-of-concept experiment on the viscosity dependence of FP of fluorescein sample in water-ethylene glycol solution and another experiment on the FP immunoassay of prostaglandin E2 sample. The measurement principle of FP is based on the synchronization between the orientation of the liquid crystal molecules and the sampling frequency of a CCD. This report is the first description of the simultaneous FP measurement of multiple samples. This system has a great potential for equipment miniaturization and price reduction as well as providing simultaneous FP measurement of multiple samples.
Graduate School of Chemical Sciences and Engineering, Hokkaido University , Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan.
Osamu
O
Wakao
Institute of Industrial Science, The University of Tokyo , 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
Yusaku
Y
Fujii
Division of Applied Chemistry, Faculty of Engineering, Hokkaido University , Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan.
Masatoshi
M
Maeki
Division of Applied Chemistry, Faculty of Engineering, Hokkaido University , Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan.
Akihiko
A
Ishida
Division of Applied Chemistry, Faculty of Engineering, Hokkaido University , Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan.
Hirofumi
H
Tani
Department of Chemistry, Tokyo Institute of Technology , 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8551, Japan.
Akihide
A
Hibara
Division of Applied Chemistry, Faculty of Engineering, Hokkaido University , Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan.|ImPACT Research Center for Advanced Nanobiodevices, Nagoya University , Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.|Innovative Research Center for Preventive Medical Engineering (PME), Nagoya University , Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.|Institute of Innovation for Future Society, Nagoya University , Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Manabu
M
Tokeshi
D015232:Dinoprostone / Q000032:analysis; D019793:Fluorescein / Q000032:analysis; D005454:Fluorescence Polarization / Q000295:instrumentation*; D050866:Liquid Crystals / Q000737:chemistry*
D015232:Dinoprostone; D019793:Fluorescein