Simple and Sensitive Colorimetric Assay for Pb2+ Based on Glutathione Protected Ag Nanoparticles by Salt Amplification.
A simple and sensitive colorimetric assay for Pb2+ detection has been reported using glutathione protected silver nanoparticles (AgNPs) by salt amplification. The naked AgNPs aggregate under the influence of salt. Glutathione (GSH) can bind to AgNPs via Ag-S bond, helping AgNPs to against salt-induced aggregation. However, GSH binding to AgNPs can be compromised by the interaction between Pb2+ and GSH. As a result, Pb2+-mediated aggregation of AgNPs under the influence of salt is reflected by the UV-Visible spectrum, and the qualitative and quantitative detection for Pb2+ is accomplished, with the detection range 0.5-4 µM and a detection limit of 0.5 µM. At the same time, Pb2+ in real water sample is detected. Furthermore, the high selectivity and low cost of the assay means it is promising for enviromental applications.
Zhang
Z
Chen
Huidong
H
Li
Lin
L
Chu
Chenbin
C
Liu
Shenglian
S
Luo
D000327:Adsorption; D003124:Colorimetry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005978:Glutathione / Q000737:chemistry*; D007854:Lead / Q000032:analysis* / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012492:Salts / Q000737:chemistry; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
D012492:Salts; D014874:Water Pollutants, Chemical; D007854:Lead; D012834:Silver; D005978:Glutathione