Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro.
Schwann cell (SC) transplantation as a cell-based therapy can enhance peripheral and central nerve repair experimentally, but it is limited by the donor site morbidity for clinical application. We investigated weather respiratory mucosa stem cells (REMSCs), a kind of ectomesenchymal stem cells (EMSCs), isolated from rat nasal septum can differentiate into functional Schwann-like cells (SC-like cells). REMSCs proliferated quickly in vitro and expressed the neural crest markers (nestin, vimentin, SOX10, and CD44). Treated with a mixture of glial growth factors for 7 days, REMSCs differentiated into SC-like cells. The differentiated REMSCs (dREMSCs) exhibited a spindle-like morphology similar to SC cells. Immunocytochemical staining and Western blotting indicated that SC-like cells expressed the glial markers (GFAP, S100β, Galc, and P75) and CNPase. When cocultured with dREMSCs for 5 days, PC12 cells differentiated into mature neuron-like cells with long neurites. More importantly, dREMSCs could form myelin structures with the neurites of PC12 cells at 21 days in vitro. Our data indicated that REMSCs, a kind of EMSCs, could differentiate into SC-like cells and have the ability to promote the differentiation of PC12 cells and form myelin in vitro.
Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China.
Jian
J
Zhang
Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China.
Xin
X
Gao
Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China.
Hongjun
H
Zou
Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China.
Jinbo
J
Liu
School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China.
Zhijian
Z
Zhang