Suppression of LPS-induced epithelial-mesenchymal transition by aqueous extracts of Prunella vulgaris through inhibition of the NF-κB/Snail signaling pathway and regulation of EMT-related protein expression.
Epithelial-mesenchymal transition (EMT) is a pivotal event in the invasion and metastasis of cancer cells. Prunella vulgaris (PV) inhibits the proliferation of various cancer cells; however, its possible role in EMT has not been demonstrated. In the present study, we explored the effect of PV aqueous extract (PVAE), a typical medicine for decoction, on EMT. Lipopolysaccharide (LPS) induced EMT-like phenotype changes in cancer cell lines that enhanced cell migration and invasion. PVAE markedly inhibited these effects and produced accompanying changes in the expression of EMT markers, including decreased expression of N-cadherin and vimentin, and increased expression of β-catenin. We found that PVAE effects on LPS-induced EMT were mediated by inhibition of the NF-κB/Snail signaling pathway. Our findings provide new evidence that PVAE suppresses cancer invasion and migration by inhibiting EMT. Therefore, we suggest that PVAE is an effective dietary chemopreventive agent with antimetastatic activity against malignant tumors.
Department of Pharmacy, Graduate School, Kyung Hee University, Seoul 130-701, Republic of Korea.
In-Hye
IH
Cho
Department of Pharmacy, Graduate School, Kyung Hee University, Seoul 130-701, Republic of Korea.
Eun Hyang
EH
Jang
Department of Life and Nanopharmaceutical Science, Graduate School, Kyung Hee University, Seoul 130-701, Republic of Korea.
Darong
D
Hong
Department of Pharmacy, Graduate School, Kyung Hee University, Seoul 130-701, Republic of Korea.
Bom
B
Jung
Department of Pharmacy, Graduate School, Kyung Hee University, Seoul 130-701, Republic of Korea.
Min-Ju
MJ
Park
Department of Pharmacy, Graduate School, Kyung Hee University, Seoul 130-701, Republic of Korea.
Jong-Ho
JH
Kim
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D058750:Epithelial-Mesenchymal Transition / Q000276:immunology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D031347:Prunella / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects*; D000071250:Snail Family Transcription Factors; D012997:Solvents / Q000737:chemistry; D014157:Transcription Factors / Q000378:metabolism; D014867:Water / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010936:Plant Extracts; D000071250:Snail Family Transcription Factors; D012997:Solvents; D014157:Transcription Factors; D014867:Water