Genome-guided insight into the methylotrophy of Paracoccus aminophilus JCM 7686.
Paracoccus aminophilus JCM 7686 (Alphaproteobacteria) is a facultative, heterotrophic methylotroph capable of utilizing a wide range of C1 compounds as sole carbon and energy sources. Analysis of the JCM 7686 genome revealed the presence of genes involved in the oxidation of methanol, methylamine, dimethylamine, trimethylamine, N,N-dimethylformamide, and formamide, as well as the serine cycle, which appears to be the only C1 assimilatory pathway in this strain. Many of these genes are located in different extrachromosomal replicons and are not present in the genomes of most members of the genus Paracoccus, which strongly suggests that they have been horizontally acquired. When compared with Paracoccus denitrificans Pd1222 (type strain of the genus Paracoccus), P. aminophilus JCM 7686 has many additional methylotrophic capabilities (oxidation of dimethylamine, trimethylamine, N,N-dimethylformamide, the serine cycle), which are determined by the presence of three separate gene clusters. Interestingly, related clusters form compact methylotrophy islands within the genomes of Paracoccus sp. N5 and many marine bacteria of the Roseobacter clade.
Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw Warsaw, Poland.
Lukasz
L
Dziewit
Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw Warsaw, Poland.
Jakub
J
Czarnecki
Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw Warsaw, Poland.
Emilia
E
Prochwicz
Institute for Genome Research and Systems Biology, Center for Biotechnology (CeBiTec), Bielefeld University Bielefeld, Germany.
Daniel
D
Wibberg
Institute for Genome Research and Systems Biology, Center for Biotechnology (CeBiTec), Bielefeld University Bielefeld, Germany.
Andreas
A
Schlüter
Institute for Genome Research and Systems Biology, Center for Biotechnology (CeBiTec), Bielefeld University Bielefeld, Germany.
Alfred
A
Pühler
Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw Warsaw, Poland.
Dariusz
D
Bartosik
N,N-dimethylformamide; Paracoccus aminophilus JCM 7686; chromid; methanol; methylated amine; methylotrophy; plasmid; serine cycle