Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production.
Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.
Bioprocess Engineering, Wageningen University, Wageningen Netherlands.
Kiira S
KS
Vuoristo
Biobased Products, Wageningen University and Research Centre, Wageningen Netherlands.
Astrid E
AE
Mars
Bioprocess Engineering, Wageningen University, Wageningen Netherlands.
Stijn
S
van Loon
Bioprocess Engineering, Wageningen University, Wageningen Netherlands.
Enrico
E
Orsi
Biobased Products, Wageningen University and Research Centre, Wageningen Netherlands.
Gerrit
G
Eggink
Biobased Products, Wageningen University and Research Centre, Wageningen Netherlands.
Johan P M
JP
Sanders
Bioprocess Engineering, Wageningen University, Wageningen Netherlands.
Ruud A
RA
Weusthuis
Escherichia coli; cis-aconitate decarboxylase; codon harmonization; codon optimization; immunoresponsive gene 1; itaconic acid; metabolic engineering