Investigation of In vitro PDT Activities and In vivo Biopotential of Zinc Phthalocyanines Using (131)I Radioisotope.
Novel octylthio-containing asymmetrically substituted Zn(II) phthalocyanine (Zn(II)Pc1) and a symmetric derivative (Zn(II)Pc2) have been prepared to investigate the biological potential and ability to photosensitize singlet oxygen for photodynamic therapy applications. In this study, the singlet oxygen generation potential and in vitro photodynamic activities of these compounds have been tested. Both ZnPcs reveal to be very efficient singlet oxygen generators and promising PSs for PDT applications. In vitro PDT activities of the compounds were evaluated in EMT-6 murine mammary carcinoma and HeLa human cervix carcinoma cell lines. Moreover, Zn(II)Pc1 displayed the phototoxic effects in the mammary cancer cell line (6.25 μm concentration at 30 J/cm(2) light dose and 12.5 μm concentration at 20 J/cm(2) light dose), while Zn(II)Pc2 did not show any phototoxic effects both in two cell lines. Zn(II)Pcs were radiolabeled with (131) I in high yields. Biodistribution studies revealed that the radiolabeled Zn(II)Pc1 showed significant uptake in l. intestine, pancreas, brain, and ovary, while Zn(II)Pc2 has significant uptake in ovary and pancreas in normal rats. Hence, these Pcs derivatives could be promising candidate for tumor nuclear imaging.
Department of Energy Systems Engineering, Faculty of Technology, Mersin University, TR-33480, Tarsus, Mersin, Turkey.|Advanced Technology Research & Application Center, Mersin University, Ciftlikkoy Campus, TR-33343, Yenisehir, Mersin, Turkey.
Mine
M
Ince
Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Izmir, Bornova, 35100, Turkey.
Ozge
O
Er
Department of Energy Systems Engineering, Faculty of Technology, Mersin University, TR-33480, Tarsus, Mersin, Turkey.|Advanced Technology Research & Application Center, Mersin University, Ciftlikkoy Campus, TR-33343, Yenisehir, Mersin, Turkey.
Kasim
K
Ocakoglu
Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Izmir, Bornova, 35100, Turkey.
Fatma Yurt
FY
Lambrecht
Advanced Technology Research & Application Center, Mersin University, Ciftlikkoy Campus, TR-33343, Yenisehir, Mersin, Turkey.
Suleyman Gokhan
SG
Colak
Department of Biomedical Technology, Institute of Science, Ege University, Izmir, Bornova, 35100, Turkey.
Hale Melis
HM
Soylu
Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Bornova, 35100, Turkey.
Cagla
C
Kayabasi
Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Bornova, 35100, Turkey.
Cumhur
C
Gunduz
D000818:Animals; D045744:Cell Line, Tumor; D002855:Chromatography, Thin Layer; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D007457:Iodine Radioisotopes / Q000737:chemistry; D054833:Isoindoles; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D026082:Singlet Oxygen / Q000378:metabolism; D013696:Temperature; D014018:Tissue Distribution; D017967:Zinc Compounds
D007211:Indoles; D007457:Iodine Radioisotopes; D054833:Isoindoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; D026082:Singlet Oxygen
131I; biodistribution; cancer; photodynamic therapy; phthalocyanine; radiolabeling