Bioleaching remediation of heavy metal-contaminated soils using Burkholderia sp. Z-90.
Bioleaching is an environment-friendly and economical technology to remove heavy metals from contaminated soils. In this study, a biosurfactant-producing strain with capacity of alkaline production was isolated from cafeteria sewer sludge and its capability for removing Zn, Pb, Mn, Cd, Cu, and As was investigated. Phylogenetic analysis using 16S rDNA gene sequences confirmed that the strain belonged to Burkholderia sp. and named as Z-90. The biosurfactant was glycolipid confirmed by thin layer chromatography and Fourier-transform infrared spectroscopy. Z-90 broth was then used for bioleaching remediation of heavy metal-contaminated soils. The removal efficiency was 44.0% for Zn, 32.5% for Pb, 52.2% for Mn, 37.7% for Cd, 24.1% for Cu and 31.6% for As, respectively. Mn, Zn and Cd were more easily removed from soil than Cu, Pb and As, which was attributed to the presence of high acid-soluble fraction of Mn, Zn and Cd and high residual fraction of Cu, Pb and As. The heavy metal removal in soils was contributed to the adhesion of heavy metal-contaminated soil minerals with strain Z-90 and the formation of a metal complex with biosurfactant.
School of Metallurgy and Environment, Central South University, Changsha 410083, China; Chinese National Engineering Research Center for Control and Treatment of Heavy Metal Pollution, Changsha 410083, China.
Zhihui
Z
Yang
School of Metallurgy and Environment, Central South University, Changsha 410083, China.
Zhi
Z
Zhang
School of Metallurgy and Environment, Central South University, Changsha 410083, China; Chinese National Engineering Research Center for Control and Treatment of Heavy Metal Pollution, Changsha 410083, China.
Liyuan
L
Chai
School of Metallurgy and Environment, Central South University, Changsha 410083, China.
Yong
Y
Wang
School of Metallurgy and Environment, Central South University, Changsha 410083, China.
Yi
Y
Liu
School of Metallurgy and Environment, Central South University, Changsha 410083, China; Chinese National Engineering Research Center for Control and Treatment of Heavy Metal Pollution, Changsha 410083, China. Electronic address: xiao.53@csu.edu.cn.
Ruiyang
R
Xiao
D000327:Adsorption; D001151:Arsenic / Q000737:chemistry*; D001673:Biodegradation, Environmental; D019117:Burkholderia / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005502:Food; D006017:Glycolipids / Q000737:chemistry* / Q000378:metabolism; D019216:Metals, Heavy / Q000737:chemistry*; D012722:Sewage; D012989:Soil Pollutants / Q000737:chemistry*; D014866:Waste Products
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006017:Glycolipids; D019216:Metals, Heavy; D012722:Sewage; D012989:Soil Pollutants; D014866:Waste Products; D001151:Arsenic
Bioleaching; Biosurfactant; Burkholderiasp.; Metal complex