Deletion of Invasion Protein B in Salmonella enterica Serovar Typhimurium Influences Bacterial Invasion and Virulence.
Salmonella enterica serovar Typhimurium (S. Typhimurium) has a wide host range and causes infections ranging from severe gastroenteritis to systemic infections in human, as well as causing typhoid-like disease in murine models of infection. S. Typhimurium translocates its effector proteins through the Salmonella pathogenicity island-I (SPI-I)-encoded T3SS-I needle complex. This study focuses on invasion protein B (SipB) of S. Typhimurium, which plays an active role in SPI-I invasion efficiency. To test our hypothesis, a sipB deletion mutant was constructed through double-crossover allelic using the suicide vector pRE112ΔsipB, and its biological characteristics were analyzed. The results showed that the SipB does not affect the growth of Salmonella, but the adherence, invasion, and virulence of the mutant were significantly decreased compared with wild-type S. Typhimurium (SL1344). This research indicates that SipB is an important virulence factor in the pathogenicity of S. Typhimurium.
Animal Disease and Public Security Academician Workstation of Henan Province. The Key Lab of Animal Disease and Public Security, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China. chensongbiao@126.com.
Songbiao
S
Chen
Animal Disease and Public Security Academician Workstation of Henan Province. The Key Lab of Animal Disease and Public Security, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China. cjzhang@sina.com.
Chunjie
C
Zhang
Animal Disease and Public Security Academician Workstation of Henan Province. The Key Lab of Animal Disease and Public Security, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China.
Chengshui
C
Liao
Animal Disease and Public Security Academician Workstation of Henan Province. The Key Lab of Animal Disease and Public Security, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China.
Jing
J
Li
Animal Disease and Public Security Academician Workstation of Henan Province. The Key Lab of Animal Disease and Public Security, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China.
Chuan
C
Yu
Animal Disease and Public Security Academician Workstation of Henan Province. The Key Lab of Animal Disease and Public Security, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China.
Xiangchao
X
Cheng
Animal Disease and Public Security Academician Workstation of Henan Province. The Key Lab of Animal Disease and Public Security, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China.
Zuhua
Z
Yu
Animal Disease and Public Security Academician Workstation of Henan Province. The Key Lab of Animal Disease and Public Security, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China.
Mingliang
M
Zhang
Animal Disease and Public Security Academician Workstation of Henan Province. The Key Lab of Animal Disease and Public Security, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China.
Yang
Y
Wang
D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D004705:Endocytosis; D017353:Gene Deletion; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D012481:Salmonella Infections, Animal; D012486:Salmonella typhimurium / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D008565:Membrane Proteins; D037521:Virulence Factors; C094579:invasion protein B, Salmonella typhimurium