Generation of Tissue Sections for Screening Thy1 Mouse Lines.
New generations of Thy1-XFP transgenic mice (where XFP stands for any fluorescent protein) can now be readily generated, given the availability of core facilities or commercial providers of Thy1 pronuclear injections. Here, we provide a protocol for screening founder progeny. Transcardial perfusion is performed on 3-wk-old F1 mice that have been produced by crossing Thy1 transgenic founders and commercially obtained inbred mice. Cryosections are generated, and Thy1-driven expression is detected by histological characterization.
Petar
P
Marinković
Leanne
L
Godinho
Thomas
T
Misgeld
D000818:Animals; D019677:Artificial Gene Fusion; D016616:Cryoultramicrotomy / Q000379:methods*; D015870:Gene Expression; D005820:Genetic Testing / Q000379:methods*; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D008822:Mice, Transgenic; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D018800:Thy-1 Antigens / Q000032:analysis* / Q000235:genetics
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D018800:Thy-1 Antigens