First genetic identification of Cryptosporidium parvum subtype IIaA14G2R1in beef cattle in Brazil.
The presence of Cryptosporidium spp. in a cattle herd registered with an outbreak of diarrhea was investigated and the the molecular subtyping of Cryptosporidium parvum was characterized. Fecal samples from 85 Nellore beef cattle (Bos indicus) were collected and examined with Ziehl-Neelsen modified staining method. Fifty-four cattle (63.52%) had Cryptosporidium spp. oocysts in their feces. Fragments of genes encoding the 18S ribosomal RNA subunit and a 60-kDa glycoprotein (gp60) were amplified by nested PCR accomplished in the 11 most heavily parasitized samples, and the amplicons were sequenced. Eight of the 11 analyzed samples were positive for 18S rRNA sequences and identified monospecific infections with C. parvum. Seven samples were positive for gp60 and identified subtypes IIaA15G2R1 (6/11) and IIaA14G2R1 (1/11). This report is the first for C. parvum subtype IIaA14G2R1 in beef cattle in Brazil.
Universidade Federal de Mato Grosso do Sul, UFMS, Campo Grande, MS 79070-900, Brazil.
Rafael Pereira
RP
Heckler
Universidade Federal de Mato Grosso do Sul, UFMS, Campo Grande, MS 79070-900, Brazil.
Dyego Gonçalves Lino
DG
Borges
Universidade Federal de Mato Grosso do Sul, UFMS, Campo Grande, MS 79070-900, Brazil.
Flávia Barbieri
FB
Bacha
Universidade Federal de Mato Grosso do Sul, UFMS, Campo Grande, MS 79070-900, Brazil.
Marcel Kenzo Vilalba
MK
Onizuka
Universidade Federal de Mato Grosso do Sul, UFMS, Campo Grande, MS 79070-900, Brazil.
Letícia e Silva
Le
Teruya
Universidade Federal de Mato Grosso do Sul, UFMS, Campo Grande, MS 79070-900, Brazil.
Juliana Paniago Lordelo
JP
Neves
Universidade Federal de Mato Grosso do Sul, UFMS, Campo Grande, MS 79070-900, Brazil.
Cássia Rejane Brito
CR
Leal
Universidade Federal de Mato Grosso do Sul, UFMS, Campo Grande, MS 79070-900, Brazil.
Ricardo Antônio Amaral
RA
de Lemos
Faculdade de Medicina Veterinária-Universidade Estadual Paulista-UNESP, Araçatuba, Brazil.
Marcelo Vasconcelos
MV
Meireles
Universidade Federal de Mato Grosso do Sul, UFMS, Campo Grande, MS 79070-900, Brazil. Electronic address: fernando.borges@ufms.br.
Fernando de Almeida
Fde A
Borges
D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000469:parasitology*; D003457:Cryptosporidiosis / Q000453:epidemiology* / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary
D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S
Cryptosporidiosis; Diarrhea; IIaA14G2R1; IIaA15G2R1; Oocysts; gp60