[Antiepileptic effect of low-frequency electrical stimulation is waveform-dependent in hippocampal kindled mice].
To investigate whether the waveform of electrical stimulus affects the antiepileptic effect of focal low-frequency stimulation (LFS).



The antiepileptic effects of the LFS in sine, monophase square and biphase square waves were investigated in hippocampal kindled mice, respectively.



Compared to the control group, sine wave focal LFS (30 s) inhibited seizure stages (2.85 ± 0.27 vs 4.75 ± 0.12, P<0.05), lowered incidence of generalized seizures (53.6% vs 96.5%, P<0.01) and reduced afterdischarge durations [(16.2 2 ± 1.69)s vs (30.29 ± 1.12)s, P<0.01] in hippocampal kindled mice, while monophase or biphase square wave LFS (30 s) showed no antiepileptic effect. Monophase square LFS (15 min) inhibited seizure stages (3.58 ± 0.16, P<0.05) and incidence of generalized seizures (66.7%,P<0.01), but had weaker inhibitory effect on hippocampal afterdischarge durations than sine wave LFS. In addition, pre-treatment and 3 s but not 10 s post-treatment with sine wave LFS resulted in suppression of evoked seizures (P<0.05 or P<0.01).



The antiepileptic effect of LFS is dependent on its waveform. Sine wave may be optimal for closed-loop LFS treatment of epilepsy.
Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;2. College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
An-feng
AF
Tao
Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;2. College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Zheng-hao
ZH
Xu
Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;2. College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Cheng-hao
CH
Wu
Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;2. College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Yi
Y
Wang
Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;2. College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Wei-wei
WW
Hou
Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;2. College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Shi-hong
SH
Zhang
Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;2. College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Zhong
Z
Chen
D000818:Animals; D000927:Anticonvulsants; D004558:Electric Stimulation; D004827:Epilepsy; D006624:Hippocampus / Q000503:physiopathology*; D007696:Kindling, Neurologic; D051379:Mice; D012640:Seizures / Q000503:physiopathology*
D000927:Anticonvulsants