Imaging Acute Neuromuscular Explants from Thy1 Mouse Lines.
Because core facilities that generate transgenic founder mice for a reasonable fee are now available at most major research institutions, generating new Thy1-XFP transgenic animals (in which XFP stands for any fluorescent protein) is an option even for relatively small laboratories. Here, we provide a protocol for screening offspring of Thy1 transgenic founders. Acute neuromuscular explants are obtained from 3-wk-old F1 mice that have been produced by crossing Thy1 transgenic founders and commercially obtained inbred mice. Thy1-driven expression is detected by fluorescence microscopy.
Petar
P
Marinković
Leanne
L
Godinho
Thomas
T
Misgeld
D000818:Animals; D019677:Artificial Gene Fusion; D016616:Cryoultramicrotomy / Q000379:methods*; D015870:Gene Expression; D005820:Genetic Testing / Q000379:methods*; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D008822:Mice, Transgenic; D009132:Muscles / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D018800:Thy-1 Antigens / Q000032:analysis* / Q000235:genetics
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D018800:Thy-1 Antigens