Rapid prediction of inducible clarithromycin resistance in Mycobacterium abscessus.
We have developed a single tube TaqMan(®) real-time PCR assay that differentiates the full-length and truncated erm(41) gene to predict inducible resistance to clarithromycin in Mycobacterium abscessus. A study of 87 clinical isolates found this assay to be 90.8% concordant to conventional drug susceptibility testing results for the prediction of inducible clarithromycin drug resistance.
Wadsworth Center, New York State Department of Health, Albany, NY, USA.
Yan Chun
YC
Zhu
Wadsworth Center, New York State Department of Health, Albany, NY, USA.
Kara K
KK
Mitchell
Wadsworth Center, New York State Department of Health, Albany, NY, USA.
Elizabeth J
EJ
Nazarian
Wadsworth Center, New York State Department of Health, Albany, NY, USA.
Vincent E
VE
Escuyer
Wadsworth Center, New York State Department of Health, Albany, NY, USA. Electronic address: kimberlee.musser@health.ny.gov.
Kimberlee A
KA
Musser
D001426:Bacterial Proteins / Q000235:genetics*; D017291:Clarithromycin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D006801:Humans; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis / Q000382:microbiology; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
D001426:Bacterial Proteins; D017291:Clarithromycin
Drug susceptibility; Mycobacterium abscessus; Nontuberculous mycobacteria (NTM); Public health; Real-time PCR; Taqman; erm(41)