Separation of α-amylase inhibitors from Abelmoschus esculentus (L).Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC.
In this study, an on-line two-dimensional high-speed counter-current chromatography system based on a six-port valve was developed. Target-guided by ultrafiltration with high-performance liquid chromatography, the one-step isolation of three potential α-amylase inhibitors from Abelmoschus esculentus (L).Moench was achieved by employing the developed orthogonal system and extrusion elution mode. The purities of three potential α-amylase inhibitors were all over 95% as determined by high-performance liquid chromatography. Furthermore, UV, mass spectrometry and1H NMR spectroscopy were applied to the structural identification of the isolated three target compounds, their structures were assigned as quercetin-3-O-sophoroside (i), 5,7,3',4'-tetrahydroxy flavonol-3-O-[β-d-rhamnopyranosil-(1→2)]-β-d-glucopyranoside (ii ) and isoquercitrin (iii), respectively. The Results demonstrated that the proposed method was highly efficient to screen and isolate enzyme inhibitors from complex natural products extracts, and on-line two-dimensional high-speed counter-current chromatography can effectively increase the peak resolution of target compounds.
College of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Hualiang
H
Zeng
College of Chemistry and Chemical Engineering, Central South University, Changsha, China.|China Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
Qi
Q
Liu
College of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Jingang
J
Yu
College of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Meiling
M
Wang
College of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Miao
M
Chen
College of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Ranhao
R
Wang
College of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Xi
X
He
College of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Menghuan
M
Gao
College of Chemistry and Chemical Engineering, Central South University, Changsha, China.|Collaborative Innovation Center of Resource-conserving & Environment-friendly Society and Ecological Civilization, Changsha, China.
Xiaoqing
X
Chen
Abelmoschus esculentus (L).Moench; Diabetes; High-speed counter-current chromatography; Ultrafiltration; α-Amylase inhibitors