High-resolution non-contact measurement of the electrical activity of plants in situ using optical recording.
The limitations of conventional extracellular recording and intracellular recording make high-resolution multisite recording of plant bioelectrical activity in situ challenging. By combining a cooled charge-coupled device camera with a voltage-sensitive dye, we recorded the action potentials in the stem of Helianthus annuus and variation potentials at multiple sites simultaneously with high spatial resolution. The method of signal processing using coherence analysis was used to determine the synchronization of the selected signals. Our results provide direct visualization of the phloem, which is the distribution region of the electrical activities in the stem and leaf of H. annuus, and verify that the phloem is the main action potential transmission route in the stems of higher plants. Finally, the method of optical recording offers a unique opportunity to map the dynamic bioelectrical activity and provides an insight into the mechanisms of long-distance electrical signal transmission in higher plants.
College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China.
Dong-Jie
DJ
Zhao
College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China.
Yang
Y
Chen
College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China.
Zi-Yang
ZY
Wang
College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China.
Lin
L
Xue
State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Tong-Lin
TL
Mao
State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Yi-Min
YM
Liu
College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China.|Key Laboratory of Agricultural information acquisition technology (Beijing), Ministry of Agriculture, Beijing 100083, China.
Zhong-Yi
ZY
Wang
College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China.|Key Laboratory of Agricultural information acquisition technology (Beijing), Ministry of Agriculture, Beijing 100083, China.
Lan
L
Huang
D000200:Action Potentials / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006368:Helianthus / Q000502:physiology*; D018547:Plant Stems / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D056969:Voltage-Sensitive Dye Imaging / Q000295:instrumentation* / Q000379:methods*