Surgical Models of Gastroesophageal Reflux with Mice.
Multiple surgical procedures have been reported to induce gastroesophageal reflux in animals. Herein, we report three surgical models with mice aiming to induce reflux of gastric contents, duodenal contents or mixed contents. Surgical procedures and general principles have been described in detail. A researcher with surgical experience should be able to grasp the technique after a short period of practice. After surgery, most mice can survive and develop reflux esophagitis similar to that in humans. However, it should be noted that histological differences between mouse and human esophagus are the inherent limitations of these surgical models. If used for research on Barrett's esophagus and adenocarcinoma, these procedures may need to be combined with genetic modifications.
Department of Thoracic Surgery, Ningxia Medical University General Hospital; Cancer Research Program, North Carolina Central University.
Jinxi
J
He
Cancer Research Program, North Carolina Central University; Department of Thoracic & Cardiovascular Surgery, The First Affiliated Hospital of Chongqing Medical University; fangyucs@sina.com.
Yu
Y
Fang
Cancer Research Program, North Carolina Central University; Department of Medicine, Center for Esophageal Disease and Swallowing, Division of Gastroenterology and Hepatology, University of North Carolina at Chapel Hill; lchen@nccu.edu.
Xiaoxin
X
Chen
D000818:Animals; D013505:Digestive System Surgical Procedures; D004195:Disease Models, Animal; D004943:Esophagogastric Junction / Q000601:surgery*; D004947:Esophagus / Q000601:surgery*; D005764:Gastroesophageal Reflux; D051379:Mice; D013270:Stomach / Q000601:surgery*