Development of an agroinoculation system for full-length and GFP-tagged cDNA clones of cucumber green mottle mosaic virus.
The complete 6243-nucleotide sequence of a cucumber green mottle mosaic virus (CGMMV) isolate from bottle gourd in Zhejiang province, China, was determined. A full-length cDNA clone of this isolate was constructed by inserting the cDNA between the 35S promoter and the ribozyme in the binary plasmid pCB301-CH. A suspension of an Agrobacterium tumefaciens EHA105 clone carrying this construct was highly infectious in Nicotiana benthamiana and bottle gourd. Another infectious clone containing the green fluorescence protein (GFP) reporter gene was also successfully constructed. This study is the first report of the efficient use of agroinoculation for generating CGMMV infections.
State Key laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of Ministry of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Hongying
H
Zheng
State Key laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of Ministry of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.|College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, 321004, China.
Caili
C
Xiao
State Key laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of Ministry of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.|College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, 321004, China.
Kelei
K
Han
State Key laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of Ministry of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Jiejun
J
Peng
State Key laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of Ministry of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Lin
L
Lin
State Key laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of Ministry of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Yuwen
Y
Lu
State Key laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of Ministry of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Li
L
Xie
Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Xiaohua
X
Wu
Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Pei
P
Xu
Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Guojing
G
Li
State Key laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of Ministry of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China. jpchen2001@126.com.
Jianping
J
Chen
State Key laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of Ministry of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China. fei.yan@mail.zaas.ac.cn.
Fei
F
Yan
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D018553:Cucumis sativus / Q000821:virology; D028464:Cucurbita; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D016679:Genome, Viral; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D014026:Nicotiana / Q000821:virology; D017898:Tobamovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D018076:DNA, Complementary; D004279:DNA, Viral; D049452:Green Fluorescent Proteins