Ulvans induce resistance against plant pathogenic fungi independently of their sulfation degree.
The present work aimed to evaluate the defense responses induced by chemically sulfated ulvans in Arabidopsis thaliana plants against the phytopathogenic fungi Alternaria brassicicola and Colletotrichum higginsianum. Derivatives with growing sulfate content (from 20.9 to 36.6%) were prepared with SO3-pyridine complex in formamide. NMR and FTIR spectroscopic analyses confirmed the increase of sulfate groups after the chemical sulfation process. The native sulfated polysaccharide (18.9% of sulfate) and its chemically sulfated derivatives similarly reduced the severity of both pathogenic fungi infections. Collectively, our results suggest that ulvans induce resistance against both fungal pathogens independently of its sulfation degree.
Laboratory of Phytopathology, Federal University of Santa Catarina, 88034-001 Florianópolis, SC, Brazil. Electronic address: mateus.brusco@ufsc.br.
Mateus B
MB
de Freitas
Department of Biochemistry and Molecular Biology, Federal University of Paraná, 81531-980 Curitiba, PR, Brazil. Electronic address: lugarciaferreira@gmail.com.
Luciana G
LG
Ferreira
Laboratory of Phytopathology, Federal University of Santa Catarina, 88034-001 Florianópolis, SC, Brazil. Electronic address: carolinehawerroth@gmail.com.
Caroline
C
Hawerroth
Department of Biochemistry and Molecular Biology, Federal University of Paraná, 81531-980 Curitiba, PR, Brazil. Electronic address: eugenia.duarte@pq.cnpq.br.
Maria Eugênia R
ME
Duarte
Department of Biochemistry and Molecular Biology, Federal University of Paraná, 81531-980 Curitiba, PR, Brazil. Electronic address: mdn@ufpr.br.
Miguel D
MD
Noseda
Laboratory of Phytopathology, Federal University of Santa Catarina, 88034-001 Florianópolis, SC, Brazil. Electronic address: marciel.stadnik@ufsc.br.
Marciel J
MJ
Stadnik
D000528:Alternaria / Q000502:physiology*; D017360:Arabidopsis / Q000187:drug effects* / Q000382:microbiology* / Q000502:physiology; D020231:Colletotrichum / Q000502:physiology*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D013431:Sulfates / Q000737:chemistry*
D011134:Polysaccharides; D013431:Sulfates; C571831:ulvan
Alternaria brassicicola; Arabidopsis thaliana; Colletotrichum higginsianum; Induced resistance; Sulfate content; Ulvans