Evolutionary divergence of Ure2pA glutathione transferases in wood degrading fungi.
The intracellular systems of detoxification are crucial for the survival of wood degrading fungi. Within these systems, glutathione transferases could play a major role since this family of enzymes is specifically extended in lignolytic fungi. In particular the Ure2p class represents one third of the total GST number in Phanerochaete chrysosporium. These proteins have been phylogenetically split into two subclasses called Ure2pA and Ure2pB. Ure2pB can be classified as Nu GSTs because of shared structural and functional features with previously characterized bacterial isoforms. Ure2pA can rather be qualified as Nu-like GSTs since they exhibit a number of differences. Ure2pA possess a classical transferase activity, a more divergent catalytic site and a higher structural flexibility for some of them, compared to Nu GSTs. The characterization of four members of this Ure2pA subclass (PcUre2pA4, PcUre2pA5, PcUre2pA6 and PcUre2pA8) revealed specific functional and structural features, suggesting that these enzymes have rapidly evolved and differentiated, probably to adapt to the complex chemical environment associated with wood decomposition.
Université de Lorraine, UMR 7036 CRM(2), F-54506 Vandoeuvre-lès-Nancy, France; CNRS, UMR 7036 CRM(2), F-54506 Vandoeuvre-lès-Nancy, France.
Thomas
T
Roret
Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, F-54506 Vandoeuvre-lès-Nancy, France; INRA, UMR 1136 Interactions Arbres/Microorganismes, F-54280 Champenoux, France.
Anne
A
Thuillier
Université de Lorraine, UMR 7036 CRM(2), F-54506 Vandoeuvre-lès-Nancy, France; CNRS, UMR 7036 CRM(2), F-54506 Vandoeuvre-lès-Nancy, France.
Frédérique
F
Favier
Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, F-54506 Vandoeuvre-lès-Nancy, France; INRA, UMR 1136 Interactions Arbres/Microorganismes, F-54280 Champenoux, France.
Eric
E
Gelhaye
Université de Lorraine, UMR 7036 CRM(2), F-54506 Vandoeuvre-lès-Nancy, France; CNRS, UMR 7036 CRM(2), F-54506 Vandoeuvre-lès-Nancy, France. Electronic address: Claude.Didierjean@univ-lorraine.fr.
Claude
C
Didierjean
Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, F-54506 Vandoeuvre-lès-Nancy, France; INRA, UMR 1136 Interactions Arbres/Microorganismes, F-54280 Champenoux, France. Electronic address: Melanie.Morel@univ-lorraine.fr.
Mélanie
M
Morel-Rouhier
D000595:Amino Acid Sequence; D044822:Biodiversity; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000737:chemistry; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D007527:Isoenzymes; D008969:Molecular Sequence Data; D020075:Phanerochaete / Q000145:classification / Q000201:enzymology; D010802:Phylogeny; D011485:Protein Binding; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D014934:Wood / Q000382:microbiology
D005656:Fungal Proteins; D007527:Isoenzymes; D005982:Glutathione Transferase; D005978:Glutathione
Activity; Diversity; Glutathione transferase; Lignolytic fungi; Nu; Structure