An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative bacteria.
An improved vector for chromosomally-integrated promoter-lux fusions is described. The modified vector was tested in parallel with the unmodified vector using the well-characterized Escherichia coli araBAD promoter in the Pseudomonas aeruginosa attTn7 site. The modified mini-Tn7 showed reduced background luminescence, and increased luminescence upon induction, giving >16-fold higher induction ratio.
Department of Biological and Physical Sciences, Montana State University Billings, Billings, MT 59101, United States.
Angela
A
Glassing
Department of Biological and Physical Sciences, Montana State University Billings, Billings, MT 59101, United States. Electronic address: tlewis@msubillings.edu.
Thomas A
TA
Lewis
D019677:Artificial Gene Fusion; D004251:DNA Transposable Elements; D020869:Gene Expression Profiling / Q000379:methods*; D005827:Genetics, Microbial / Q000379:methods*; D006090:Gram-Negative Bacteria / Q000235:genetics*; D058507:Host Specificity; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D049449:Luminescence; D008967:Molecular Biology / Q000379:methods*; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation
D004251:DNA Transposable Elements; D008156:Luciferases
Luciferase; Reporter; Tn7; araBAD; lux