Genetic diversity and haplotype structure of 21 Y-STRs, including nine noncore loci, in South Tunisian Population: Forensic relevance.
Y chromosome STRs (Y-STRs) are being used frequently in forensic laboratories. Previous studies of Y-STR polymorphisms in different groups of the Tunisian population identified low levels of diversity and discrimination capacity (DC) using various commercial marker sets. This definitely limits the use of such systems for Y-STRs genotyping in Tunisia. In our investigation on South Tunisia, 200 unrelated males were typed for the 12 conventional Y-STRs included in the PowerPlex® Y System. Additional set of nine noncore Y-STRs including DYS446, DYS456, DYS458, DYS388, DYS444, DYS445, DYS449, DYS710, and DYS464 markers were genotyped and evaluated for their potential in improving DC. Allele frequency, gene diversity, haplotype diversity (HD), and DC calculation revealed that DYS464 was the most diverse marker followed by DYS710 and DYS449 markers. The standard panel of 12 Y-STRs (DC = 80.5%) and the nine markers were combined to obtain DC of 99%. Among the 198 different haplotypes observed, 196 haplotypes were unique (HD = 99.999). Out of the nine noncore set, six Y-STRs (DYS458, DYS456, DYS449, DYS710, DYS444, and DYS464) had the greatest impact on enhancing DC. Our data provided putative Y-STRs combination to be used for genetic and forensic applications.
Service Laboratoire, Unité Empreinte Génétique, CHU Hedi Chaker, Sfax, Tunisie.|Laboratoire Procédés de Criblage Moléculaire et Cellulaire, Centre de Biotechnologie de Sfax, Université de Sfax, Sfax, Tunisie.
Faten
F
Makki-Rmida
Service Laboratoire, Unité Empreinte Génétique, CHU Hedi Chaker, Sfax, Tunisie.
Arwa
A
Kammoun
Service Laboratoire, Unité Empreinte Génétique, CHU Hedi Chaker, Sfax, Tunisie.
Nadia
N
Mahfoudh
Service Médicine Légale, CHU Habib Bourguiba, Tunisie.
Adnene
A
Ayadi
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, British University in Egypt, Cairo, Egypt.
Abdullah Ahmed
AA
Gibriel
Service Laboratoire, Unité Empreinte Génétique, CHU Hedi Chaker, Sfax, Tunisie.
Bakhta
B
Mallek
Service Laboratoire, Unité Empreinte Génétique, CHU Hedi Chaker, Sfax, Tunisie.
Leila
L
Maalej
Service Médicine Légale, CHU Habib Bourguiba, Tunisie.
Zouheir
Z
Hammami
Service Médicine Légale, CHU Habib Bourguiba, Tunisie.
Samir
S
Maatoug
Service Laboratoire, Unité Empreinte Génétique, CHU Hedi Chaker, Sfax, Tunisie.
Hafedh
H
Makni
Laboratoire Procédés de Criblage Moléculaire et Cellulaire, Centre de Biotechnologie de Sfax, Université de Sfax, Sfax, Tunisie.
Saber
S
Masmoudi
D041322:Chromosomes, Human, Y; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D014416:Tunisia
Discrimination capacity; Forensic; Haplotype diversity; Tunisian population; Y-STRs