Efficacy of Tolypocladium cylindrosporum against Aedes aegypti eggs, larvae and adults.
OBJECTIVE
The aim of this study was to test the activity of seven Tolypocladium cylindrosporum isolates against different developmental stages of Aedes aegypti.


RESULTS
Ovicidal activity (≤30% of eclosion; at a 84% eclosion of control eggs) and lowest cumulative emergence of adults (≤30%; at a 66·3% control emergence) originating from eclosing larvae was found 15 and 30 days, respectively, after applying conidia of ARSEF 962 or 1580 onto the eggs. All isolates induced larval mortality when third-instar larvae were treated with conidia (≤5% control mortality); the lowest lethal concentration (LC50) at 5 days after treatment was found with ARSEF 1580 (9·2 × 10(5) conidia ml(-1)), and the shortest lethal time (LT50 ) with ARSEF 2912 (2·8 days at 3·3 × 10(6) conidia ml(-1)). The pathogenicity of T. cylindrosporum to A. aegypti adults is corroborated. ARSEF 1027, 1580 and 2912 induced the highest cumulative mortality in adults (≥90%) 15 days after application (≤5% control mortality).


CONCLUSIONS
Findings emphasized an isolate- and developmental stage-related virulence of T. cylindrosporum.


CONCLUSIONS
ARSEF 1580, in the group of tested isolates, is the most promising isolate for use against terrestrial eggs, aquatic larvae and adults of this important vector.
Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás, Goiânia, Brazil.|Instituto Federal de Educação, Ciência e Tecnologia de Goiás, (IFG), Aparecida de Goiânia, Brazil.
L F N
LF
Rocha
Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás, Goiânia, Brazil.
N A
NA
Sousa
Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás, Goiânia, Brazil.
J
J
Rodrigues
Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás, Goiânia, Brazil.
A M L
AM
Catão
Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás, Goiânia, Brazil.
C S
CS
Marques
Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás, Goiânia, Brazil.
É K K
ÉK
Fernandes
Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás, Goiânia, Brazil.
C
C
Luz
D000330:Aedes / Q000254:growth & development / Q000382:microbiology*; D000818:Animals; D005260:Female; D006999:Hypocreales / Q000502:physiology*; D007814:Larva / Q000187:drug effects / Q000382:microbiology*; D008297:Male; D010063:Ovum / Q000382:microbiology; D010572:Pest Control, Biological / Q000379:methods*; D013172:Spores, Fungal / Q000502:physiology
Hypocreales; biological control; entomopathogenic fungus; isolate; mosquito