Characterization of polysaccharides with antioxidant and immunological activities from Rhizoma Acori Tatarinowii.
Rhizoma Acori Tatarinowii is widely used in traditional Chinese medicine. In this study, three novel polysaccharides designated RATPW, RATPS1 and RATPS2 were isolated from Rhizoma Acori Tatarinowii by DEAE-52 cellulose chromatography. Their structures were characterized using physicochemical and spectral methods. Chemical analysis indicated that RATPW (6.5×10(3)Da) mainly composed of glucose and fructose. RATPS1 (1.5×10(5)Da) contained galactose and arabinose, while RATPS2 (5.3×10(4)Da) contained ∼49.5% galacturonic acid along with rhamnose, fructose, galactose, and arabinose. In vitro, RATPS2 showed the most significant scavenging activity on DPPH and hydroxyl radical. Three polysaccharides could protect the PC12 cells from H2O2-induced damage. Immunological tests indicated that both RATPW and RATPS2 significantly stimulated NO production and phagocytic activity in RAW264.7, and promoted splenocyte proliferation. These data suggested that polysaccharides RATPW and RATPS2 had the potential as novel natural sources of antioxidative and immunopotentiating agents.
College of Science, Northwest A&F University, Yangling 712100, Shaanxi, China.
Wuxia
W
Zhang
College of Science, Northwest A&F University, Yangling 712100, Shaanxi, China.
Dan
D
Song
College of Science, Northwest A&F University, Yangling 712100, Shaanxi, China.
Dan
D
Xu
College of Science, Northwest A&F University, Yangling 712100, Shaanxi, China.
Tingting
T
Wang
College of Science, Northwest A&F University, Yangling 712100, Shaanxi, China.
Lu
L
Chen
College of Science, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: jduan@nwsuaf.edu.cn.
Jinyou
J
Duan
D029023:Acorus / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009569:Nitric Oxide / Q000096:biosynthesis; D016716:PC12 Cells; D010587:Phagocytosis / Q000187:drug effects; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000067996:RAW 264.7 Cells; D051381:Rats; D027343:Rhizome / Q000737:chemistry; D013154:Spleen / Q000166:cytology
D000975:Antioxidants; D001713:Biphenyl Compounds; D007155:Immunologic Factors; D010851:Picrates; D011134:Polysaccharides; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl
Antioxidative activity; Immunological activity; Polysaccharides; Rhizoma Acori Tatarinowii