Isolation and Characterization of Polymorphic Microsatellite Markers from the Malaria Vector Anopheles fluviatilis Species T (Diptera: Culicidae).
Anopheles fluviatilis James is an important malaria vector in India, Pakistan, Nepal, and Iran. It has now been recognized as a complex of at least four sibling species-S, T, U, and V, among which species T is the most widely distributed species throughout India. The taxonomic status of these species is confusing owing to controversies prevailing in the literature. In addition, chromosomal inversion genotypes, which were considered species-diagnostic for An. fluviatilis species T, are unreliable due to the existence of polymorphism in some populations. To study the genetic diversity at population level, we isolated and characterized 20 microsatellite markers from microsatellite-enriched genomic DNA library of An. fluviatilis T, of which 18 were polymorphic while two were monomorphic. The number of alleles per locus among polymorphic markers ranged from 4 to 19, and values for observed and expected heterozygosities varied from 0.352 to 0.857 and from 0.575 to 0.933, respectively. Thirteen markers had cross-cryptic species transferability to species S and U of the Fluviatilis Complex. This study provides a promising genetic tool for the population genetic analyses of An. fluviatilis.
National Institute of Malaria Research (NIMR), Sector-8, Dwarka, New Delhi-110077, India.
Manila
M
Lather
National Institute of Malaria Research (NIMR), Sector-8, Dwarka, New Delhi-110077, India.
Divya
D
Sharma
Maharshi Dayanand University (MDU), Rohtak-124001 (Haryana), India.
Amita S
AS
Dang
National Institute of Malaria Research (NIMR), Sector-8, Dwarka, New Delhi-110077, India.
Tridibes
T
Adak
National Institute of Malaria Research (NIMR), Sector-8, Dwarka, New Delhi-110077, India. Singh@mrcindia.org.
Om P
OP
Singh
D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000378:metabolism; D051456:Gene Flow; D005819:Genetic Markers; D007194:India; D007303:Insect Vectors / Q000235:genetics* / Q000378:metabolism; D008288:Malaria / Q000469:parasitology; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D005819:Genetic Markers
Anopheles fluviatilis T; malaria; microsatellite marker; population genetics