Thyroid hormone modulates neuroglobin and cytoglobin in rat brain.
Thyroid hormones (THs) are essential and crucial for brain development, playing a role in growth and differentiation. Two globins named neuroglobin (Ngb) and cytoglobin (Cygb) are located in the brain, and each one has different distribution and function: They seem to have similar action by providing O(2) for respiratory chain, and detoxification of reactive oxygen species (ROS) and nitric oxide (NO) protecting tissues against irreversible lesions. We aimed to investigate the influence of thyroid state in Ngb and Cygb metabolism in different brain regions and evaluate their responses in cerebellum, hippocampus and cerebral cortex (hereafter called as cortex) after supraphysiological doses at different time points of TH administration. Experiments were carried out in rats, divided in eight experimental groups Control (C), thyroidectomy (Tx), and thyroidectomy treated with jugular intravenous injection (i.v). T3 (100 μl/100 g) injection and sacrificed after 30, 60, 120 min and 6, 12 and 24 h. In cortex, we found increase in Ngb gene and protein expression in different time points compared to C group, however Cygb gene and protein expression were decreased. In hippocampus, Ngb and Cygb protein expression increased 24 h after i.v. T3 injection in comparison to Tx. In cerebellum, we found increased Ngb gene expression after 120 min, 6, 12 and 24 h after T3 administration compared to Tx, and in contrast, protein expression was found to be significantly increased only 12 and 24 h compared to Tx. Ngb and Cygb expression in brain is influenced by thyroid hormone state both by its lack or excess.
Department Morphology and Physiology, Faculdade de Medicina do ABC, Santo Andre, SP, Brazil.|Department Medicine, Universidade Federal de Sao Paulo (UNIFESP), Rua Pedro de Toledo, Vila Clementino, Sao Paulo, SP, 04039032, Brazil.
Kelen Carneiro
KC
Oliveira
Department Medicine, Universidade Federal de Sao Paulo (UNIFESP), Rua Pedro de Toledo, Vila Clementino, Sao Paulo, SP, 04039032, Brazil.
Rodrigo Rodrigues
RR
da Conceição
Department Physiology and Biophysics, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
Gisele Constantinov
GC
Piedade
Department Medicine, Universidade Federal de Sao Paulo (UNIFESP), Rua Pedro de Toledo, Vila Clementino, Sao Paulo, SP, 04039032, Brazil.
Janaina Sena
JS
de Souza
Department Morphology and Physiology, Faculdade de Medicina do ABC, Santo Andre, SP, Brazil.
Monica Akemi
MA
Sato
Department Medicine, Universidade Federal de Sao Paulo (UNIFESP), Rua Pedro de Toledo, Vila Clementino, Sao Paulo, SP, 04039032, Brazil.
Rui Monteiro
RM
de Barros Maciel
Department Morphology and Physiology, Faculdade de Medicina do ABC, Santo Andre, SP, Brazil. ggiannocco@gmail.com.|Department Medicine, Universidade Federal de Sao Paulo (UNIFESP), Rua Pedro de Toledo, Vila Clementino, Sao Paulo, SP, 04039032, Brazil. ggiannocco@gmail.com.|Department Biological Sciences, Universidade Federal de Sao Paulo, Diadema, SP, Brazil. ggiannocco@gmail.com.
Gisele
G
Giannocco
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology*; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D000078842:Cytoglobin; D005914:Globins / Q000096:biosynthesis* / Q000235:genetics*; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics*; D000078787:Neuroglobin; D051381:Rats; D017208:Rats, Wistar; D013963:Thyroid Hormones / Q000097:blood / Q000494:pharmacology / Q000502:physiology*; D013965:Thyroidectomy; D014284:Triiodothyronine / Q000494:pharmacology
C000627268:Cygb protein, rat; D000078842:Cytoglobin; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D013963:Thyroid Hormones; D014284:Triiodothyronine; D005914:Globins
Cerebellum; Cerebral cortex; Cytoglobin; Hippocampus; Neuroglobin; Thyroid hormones