Short Communication: Investigating a Chain of HIV Transmission Events Due to Homosexual Exposure and Blood Transfusion Based on a Next Generation Sequencing Method.
This study investigates a chain of HIV transmission events due to homosexual exposure and blood transfusion in China. The MiSeq platform, a next generation sequencing (NGS) system, was used to obtain genetic details of the HIV-1 env region (336 base pairs). Evolutionary analysis combined with epidemiologic evidence suggests a transmission chain from patient T3 to T2 through homosexual exposure and subsequently to T1 through blood transfusion. More importantly, a phylogenetic study suggested a likely genetic bottleneck for HIV in homosexual transmission from T3 to T2, while T1 inherited the majority of variants from T2. The result from the MiSeq platform is consistent with findings from the epidemiologic survey. The MiSeq platform is a powerful tool for tracing HIV transmissions and intrapersonal evolution.
1 National AIDS Reference Laboratory, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, China .
Qi
Q
Zhao
2 National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, China .
Chen
C
Zhang
1 National AIDS Reference Laboratory, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, China .
Yan
Y
Jiang
1 National AIDS Reference Laboratory, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, China .
Yujie
Y
Wen
1 National AIDS Reference Laboratory, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, China .
Pinliang
P
Pan
2 National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, China .
Yang
Y
Li
1 National AIDS Reference Laboratory, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, China .
Guiyun
G
Zhang
3 Research Center for Public Health, School of Medicine, Tsinghua University , Beijing, China .|4 Melbourne Sexual Health Centre , Alfred Health, Melbourne, Victoria, Australia .|5 Central Clinical School, Faculty of Medicine, Nursing and Health Sciences, Monash University , Melbourne, Victoria, Australia .
Lei
L
Zhang
1 National AIDS Reference Laboratory, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, China .
Maofeng
M
Qiu
D002681:China / Q000453:epidemiology; D003075:Coitus; D018562:Disease Transmission, Infectious; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology / Q000635:transmission* / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D059014:High-Throughput Nucleotide Sequencing; D006716:Homosexuality; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D010802:Phylogeny; D065227:Transfusion Reaction; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics
D054299:env Gene Products, Human Immunodeficiency Virus