neo-Clerodane Diterpenoids from Scutellaria barbata and Their Inhibitory Effects on LPS-Induced Nitric Oxide Production.
Three new neo-clerodane diterpenoids (1-3) along with 12 known compounds (4-15) were isolated from a methanol extract of the aerial parts of Scutellaria barbata. The structures of 1-3 were determined by interpretation of their 1D and 2D NMR spectroscopic data as well as HRESIMS values. All isolated compounds were tested for their inhibitory effects on LPS-induced nitric oxide production in RAW 264.7 macrophages. Compounds 1-4, 7, and 10-12 were found to inhibit nitric oxide production with IC50 values ranging from 20.2 to 35.6 μM.
College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
Eung Tae
ET
Yeon
College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
Jin Woo
JW
Lee
College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
Chul
C
Lee
College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
Qinghao
Q
Jin
College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
Hari
H
Jang
Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Seoul 02841, Korea.
Dongho
D
Lee
Korea Research Institute of Bioscience and Biotechnology , Cheongju 28116, Korea.
Jong Seog
JS
Ahn
College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
Jin Tae
JT
Hong
College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
Youngsoo
Y
Kim
College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
Mi Kyeong
MK
Lee
College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
Bang Yeon
BY
Hwang
D045785:Diterpenes, Clerodane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis*; D019906:Nuclear Magnetic Resonance, Biomolecular; D035261:Plant Components, Aerial / Q000737:chemistry; D031363:Scutellaria / Q000737:chemistry*
D045785:Diterpenes, Clerodane; D008070:Lipopolysaccharides; C000603559:scutebatin A; C000603560:scutebatin B; C000603561:scutebatin C; D009569:Nitric Oxide