Total Glucosides of Danggui Buxue Tang Attenuate BLM-Induced Pulmonary Fibrosis via Regulating Oxidative Stress by Inhibiting NOX4.
Pulmonary fibrosis (PF) is a serious chronic lung disease with unknown pathogenesis. Researches have confirmed that oxidative stress which is regulated by NADPH oxidase-4 (NOX4), a main source of reactive oxygen species (ROS), is an important molecular mechanism underlying PF. Previous studies showed that total glucosides of Danggui Buxue Tang (DBTG), an extract from a classical traditional Chinese herbal formula, Danggui Buxue Tang (DBT), attenuated bleomycin-induced PF in rats. However, the mechanisms of DBTG are still not clear. We hypothesize that DBTG attenuates PF through regulating the level of oxidative stress by inhibiting NOX4. And we found that fibrosis indexes hydroxyproline (HYP) and type I collagen (Col-I) were lower in DBTG groups compared with the model group. In addition, the expression of transforming growth factor-β1 (TGF-β1) and expression of alpha smooth muscle actin (α-SMA) were also much more decreased than the model group. For oxidative stress indicators, DBTG blunted the decrease of superoxide dismutase (SOD) activity, total antioxidant capacity (T-AOC), and the increase in malondialdehyde (MDA), 8-iso-prostaglandin in lung homogenates. Treatment with DBTG restrained the expression of NOX4 compared to the model group. Present study confirms that DBTG inhibits BLM-induced PF by modulating the level of oxidative stress via suppressing NOX4.
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230038, China.
Ping
P
Zhao
The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China ; School of Pharmacy, Anhui Medical University, Hefei 230032, China.
Wen-Cheng
WC
Zhou
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230038, China.
De-Lin
DL
Li
School of Pharmacy, Anhui Medical University, Hefei 230032, China.
Xiao-Ting
XT
Mo
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230038, China.
Liang
L
Xu
School of Pharmacy, Anhui Medical University, Hefei 230032, China.
Liu-Cheng
LC
Li
School of Pharmacy, Anhui Medical University, Hefei 230032, China.
Wen-Hui
WH
Cui
The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
Jian
J
Gao
D000818:Animals; D001688:Biological Products / Q000009:adverse effects*; D001761:Bleomycin / Q000008:administration & dosage / Q000009:adverse effects*; D004365:Drugs, Chinese Herbal / Q000009:adverse effects*; D005960:Glucosides / Q000378:metabolism*; D006801:Humans; D000074663:NADPH Oxidase 4; D019255:NADPH Oxidases / Q000378:metabolism*; D018384:Oxidative Stress; D011658:Pulmonary Fibrosis / Q000139:chemically induced* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species
D001688:Biological Products; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D017382:Reactive Oxygen Species; C477279:danggui buxue decoction; D001761:Bleomycin; D000074663:NADPH Oxidase 4; D019255:NADPH Oxidases; C498910:NOX4 protein, human