Moxifloxacin superior to cefuroxime in reducing bacterial adhesion of Staphylococcus epidermidis on hydrophobic intraocular lenses.
OBJECTIVE
To compare the anti-adhesive effect of cefuroxime and moxifloxacin on the primary attachment phase of Staphylococcus epidermidis on hydrophobic acrylic intraocular lenses (IOLs).


METHODS
Forty hydrophobic acrylic IOLs were used. Two groups of IOLs were soaked in a moxifloxacin (Mox-T1: 0.5 mg/0.1 ml) or a cefuroxime (Cef-T1: cefuroxime 1 mg/0.1 ml) solution before incubation in a S. epidermidis bacterial suspension. Two other groups were incubated in the bacterial suspension before antibiotics (Cef-T2 and Mox-T2) were added. The control group (Ctrl) consisted of IOLs incubated in the bacterial suspension. After incubation, IOLs were sonicated and vortexed. The resultant suspension was spread over a nutritive agar plate. Bacterial colonies were counted after 24 hr of incubation.


RESULTS
Mean number of colony-forming units per IOL was Cef-T1: 184 × 10(3) (SE: 5.24; SD: 28.21), Cef-T2: 117 × 10(3) (SE: 5.74; SD: 30.37), Mox-T1: 1.27 × 10(3) (SE: 0.12; SD: 0.61), Mox-T2: 25 × 10(3) (SE:1.98; SD: 9.72) and Ctrl: 361 × 10(3) (SE: 26.9; SD: 107.6). The number of adhering bacteria did not vary whether cefuroxime was added before or after IOL incubation in the bacterial suspension (p = 0.132). Moxifloxacin was more effective in reducing the number of adhering bacteria when used before IOL incubation (p < 0.001). Overall for T1 and T2, moxifloxacin was more effective than cefuroxime in reducing bacterial adhesion on IOLs (p < 0.001).


CONCLUSIONS
Moxifloxacin and cefuroxime significantly reduced S. epidermidis adhesion on hydrophobic acrylic IOLs. The anti-adhesive effect was superior with moxifloxacin.
Department of Ophthalmology, La Croix Rousse University Hospital, Lyon, France.|UMR CNRS 5510/MATEIS team I2B 'Biomaterials and Biological Interactions', Villeurbanne, France.
Fathalah
F
Benbouzid
Department of Ophthalmology, La Croix Rousse University Hospital, Lyon, France.|UMR CNRS 5510/MATEIS team I2B 'Biomaterials and Biological Interactions', Villeurbanne, France.
Laurent
L
Kodjikian
UMR CNRS 5510/MATEIS team I2B 'Biomaterials and Biological Interactions', Villeurbanne, France.|Faculty of Pharmacy, Pharmaceutics and Biological Sciences Institute, Lyon, France.
Daniel
D
Hartmann
UMR CNRS 5510/MATEIS team I2B 'Biomaterials and Biological Interactions', Villeurbanne, France.|Microbiology Laboratory, Pharmaceutics and Biological Sciences Institute, Lyon, France.
François
F
Renaud
UMR CNRS 5510/MATEIS team I2B 'Biomaterials and Biological Interactions', Villeurbanne, France.|Department of Ophthalmology, Saint Roch University Hospital, Nice, France.
Stéphanie
S
Baillif
D000180:Acrylic Resins; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D002444:Cefuroxime / Q000494:pharmacology*; D015169:Colony Count, Microbial; D024841:Fluoroquinolones / Q000494:pharmacology*; D057927:Hydrophobic and Hydrophilic Interactions; D007910:Lenses, Intraocular / Q000382:microbiology*; D000077266:Moxifloxacin; D013212:Staphylococcus epidermidis / Q000502:physiology*
D000180:Acrylic Resins; D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D002444:Cefuroxime; D000077266:Moxifloxacin
bacterial adhesion; cefuroxime; endophthalmitis; intraocular lens; moxifloxacin