[Genome-wide identification and analyses of 4CL gene families in Pyrus bretschneideri Rehd].
4-coumaric acid: coenzyme A ligase (4CL) gene is one of the key genes involved in the regulation of lignin metabolism and the synthesis of flavonoid and other secondary metabolites in plant, while the synthesized and polymerized lignin is deposited in cell walls and leads to thickening of secondary walls in some parenchyma cells and formation of stone cells. To better understand the variety and quantity of 4CL genes in Pyrus bretschneideri Rehd., we used the amino acid and cDNA databases of Pyrus bretschneideri Rehd. genome to screen 4CL gene family, and analyzed their classification, evolutionary relationships, physical location, gene structure and conserved motif. Our results showed that 29 4CL genes were identified and preliminarily characterized, and these 4CL genes were distributed in all chromosomes except chromosomes 4, 8, 11, 12 and clustered on chromosomes 9 and 17 through gene location analysis. The relationship between 4CL gene structure and evolution was further determined by comparing gene structure and phylogenetic tree. These findings provide a basis for further analysis of 4CL gene function in Pyrus bretschneideri Rehd.
College of Chemistry and Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Yun-peng
YP
Cao
College of Chemistry and Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Zhi
Z
Fang
College of Chemistry and Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Shu-mei
SM
Li
College of Chemistry and Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Chong-chong
CC
Yan
College of Chemistry and Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Qing-qing
QQ
Ding
College of Chemistry and Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Xi
X
Cheng
College of Chemistry and Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Yi
Y
Lin
College of Chemistry and Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Ning
N
Guo
College of Chemistry and Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Yong-ping
YP
Cai
D000595:Amino Acid Sequence; D003066:Coenzyme A Ligases / Q000235:genetics*; D018745:Genome, Plant; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D031989:Pyrus / Q000235:genetics*
D003066:Coenzyme A Ligases; C019943:4-coumarate-CoA ligase