Field use of ultrasonography to characterize the reproductive tract and early pregnancy in a phocid, the Weddell seal (Leptonychotes weddellii).
The utility of transrectal ultrasonography was tested in a field setting to characterize the reproductive tract and detect early pregnancy (embryonic vesicles < 3 mm in diameter) in 17 multiparous female Weddell seals (Leptonychotes weddellii). Female Weddell seals give birth in October/November each year, followed by the breeding season (December) and embryonic diapause. Transrectal ultrasonography was attempted in January/February 2014 to examine the entire reproductive tract (uterine horns and body, ovaries with follicles and corpora lutea) using a 5-10 MHz linear-array probe with a 70 cm-long extension. A single pregnancy was detected in 14 of 17 seals (82.4%) as a circular or guitar-pick shaped nonechogenic (black) vesicle with a clearly visible echogenic border within the lumen of the uterus. The stage of embryonic development (ostensibly the gestational age) varied markedly among individuals, ranging from a vesicle with no embryo proper to a large fetus with an ocular orbit, nose/mouth, limb buds, spinal column, umbilical cord, and prominent vasculature. Two of the pregnant seals were re-examined 3-6 days after the initial examination to obtain longitudinal growth rates. Images of one or both ovaries were obtained in nine and seven of the 19 examinations, respectively. Numerous ovarian follicles (3 to 12 mm diameter) were detected and animals typically had a single well-perfused corpus luteum (determined by Doppler color-flow) ipsilateral to the uterine horn containing the pregnancy. We conclude that real-time transrectal ultrasonography is an effective tool for characterizing reproductive events in phocids including ovarian dynamics, and for elucidating the nature of embryonic diapause.
Biological Sciences, University of Alaska Anchorage, Anchorage, Alaska.|School of Fisheries and Ocean Sciences, University of Alaska Fairbanks, Fairbanks, Alaska.
Michelle R
MR
Shero
Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Gregg P
GP
Adams
Biological Sciences, University of Alaska Anchorage, Anchorage, Alaska.
Jennifer M
JM
Burns
D000818:Animals; D003338:Corpus Luteum / Q000033:anatomy & histology / Q000000981:diagnostic imaging; D005260:Female; D006080:Ovarian Follicle / Q000033:anatomy & histology / Q000000981:diagnostic imaging; D010053:Ovary / Q000033:anatomy & histology* / Q000000981:diagnostic imaging*; D011247:Pregnancy; D012098:Reproduction; D046048:Seals, Earless / Q000033:anatomy & histology*; D014463:Ultrasonography; D014599:Uterus / Q000033:anatomy & histology* / Q000000981:diagnostic imaging*
corpus luteum; diapause; embryo; ovary; pinniped; pregnancy; reproductive tract; ultrasonography