Novel plasmid-borne multidrug resistance gene cluster including lsa(E) from a linezolid-resistant Enterococcus faecium isolate of swine origin.
A novel nonconjugative plasmid of 28,489 bp from a porcine linezolid-resistant Enterococcus faecium isolate was completely sequenced. This plasmid harbored a novel type of multiresistance gene cluster that comprised the resistance genes lnu(B), lsa(E), spw, aadE, aphA3, and two copies of erm(B), which account for resistance to macrolides, lincosamides, streptogramins, pleuromutilins, streptomycin, spectinomycin, and kanamycin/neomycin. Structural comparisons suggested that this plasmid might have developed from other enterococcal plasmids by insertion element (IS)-mediated interplasmid recombination processes.
College of Animal Sciences and Technology, Guangxi University, Nanning, China.
Hongbin
H
Si
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Wan-Jiang
WJ
Zhang
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Shengbo
S
Chu
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Xiu-Mei
XM
Wang
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.
Lei
L
Dai
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Xin
X
Hua
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Zhimin
Z
Dong
Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany siguo_liu@hvri.ac.cn stefan.schwarz@fli.bund.de.
Stefan
S
Schwarz
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China siguo_liu@hvri.ac.cn stefan.schwarz@fli.bund.de.
Siguo
S
Liu
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004224:Diterpenes / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D016984:Enterococcus faecium / Q000187:drug effects* / Q000235:genetics*; D055231:Lincosamides / Q000494:pharmacology; D000069349:Linezolid / Q000494:pharmacology; D018942:Macrolides / Q000494:pharmacology; D051379:Mice; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D011083:Polycyclic Compounds; D025361:Streptogramins / Q000494:pharmacology; D013552:Swine; D000097567:Pleuromutilins
D000900:Anti-Bacterial Agents; D004224:Diterpenes; D055231:Lincosamides; D000069349:Linezolid; D018942:Macrolides; D011083:Polycyclic Compounds; D025361:Streptogramins; D000097567:Pleuromutilins