Comparison of the effects of Origanum vulgare with LHRH-A2 and 17β-estradiol on the ultrastructure of gonadotroph cells and ovarian oogenesis in immature Trichogaster trichopterus.
Origanum vulgare is a plant of the mint family that contains phytoestrogens. This study compared the effects of O. vulgare, LHRH-A2, and 17β-estradiol on the ultrastructure of gonadotroph cells and ovarian oogenesis in immature Trichogaster trichopterus. Fish (5.1±0.032cm and 2.1±0.043g, n=150) were randomly divided into four treatment groups (three hormonal treatments and control) and treated intramuscularly at four levels with 17β-estradiol or O. vulgare at 10, 20, 30 and 50mg/kg body weight and with LHRH-A2 at 0.001, 0.002, 0.003, and 0.005mg/kg body weight. There were three control treatments: saline, ethanol and placebo. Fish were kept in 15 tanks, with 10 fish per tank, injected a total of seven doses over 13 days. Gonadosomatic index (GSI) and oocyte diameter were lower (P≤0.05) in the control than in the three hormonal treatments. The highest GSI and oocyte diameter responses were observed in fish treated with 17β-estradiol (2.76±0.23%, 149.8±15.43mm) followed by O. vulgare (1.86±0.18%, 104.3±11.5mm) and LHRH-A2 (1.52±0.12%, 91.75±9.02mm) (P≤0.05). Moreover, there was a significant effect of dose level within all the hormonal treatments (P≤0.05). The effect of treatment on the length and weight was likely GSI. Ovarian tissue results showed faster oogenesis of oocytes in fish treated with O. vulgare, after 17β-estradiol. Ultrastructure of gonadotroph cells demonstrated less stimulation by O. vulgare than by 17β-estradiol and LHRH-A2. This study suggests that compared with the two hormonal treatments, O. vulgare dose-dependently affects ovarian oogenesis and gonadotroph cells.
Department of Basic Sciences, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran. Electronic address: sebagheri2000@gmail.com.
Sedigheh
S
Bagheri Ziari
Department of Basic Sciences, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran.
Tahereh
T
Naji
Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran.
Homayoun
H
Hosseinzadeh Sahafi
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology*; D052681:Gonadotrophs / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology; D009866:Oogenesis / Q000187:drug effects* / Q000502:physiology; D031341:Origanum; D010473:Perciformes / Q000502:physiology*; D048789:Phytoestrogens / Q000494:pharmacology*
C000595897:(des-Gly10,D-Ala6)-LHRH ethylamide; D048789:Phytoestrogens; D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol
17β-Estradiol; Gonadotroph cells; LHRH-A(2); Oogenesis; Origanum vulgare