Oral Bioavailability, Hydrolysis, and Comparative Toxicokinetics of 3-Acetyldeoxynivalenol and 15-Acetyldeoxynivalenol in Broiler Chickens and Pigs.
The goal of this study was to determine the absolute oral bioavailability, (presystemic) hydrolysis and toxicokinetic characteristics of deoxynivalenol, 3-acetyldeoxynivalenol, and 15-acetyldeoxynivalenol in broiler chickens and pigs. Crossover animal trials were performed with intravenous and oral administration of deoxynivalenol, 3-acetyldeoxynivalenol, and 15-acetyldeoxynivalenol to broilers and pigs. Plasma concentrations were analyzed by using liquid chromatography-tandem mass spectrometry, and data were processed via a tailor-made compartmental toxicokinetic analysis. The results in broiler chickens showed that the absorbed fraction after oral deoxynivalenol, 3-acetyldeoxynivalenol, and 15-acetyldeoxynivalenol administration was 10.6, 18.2, and 42.2%, respectively. This fraction was completely hydrolyzed presystemically for 3-acetyldeoxynivalenol to deoxynivalenol and to a lesser extent (75.4%) for 15-acetyldeoxynivalenol. In pigs, the absorbed fractions were 100% for deoxynivalenol, 3-acetyldeoxynivalenol, and 15-acetyldeoxynivalenol, and both 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol were completely hydrolyzed presystemically. The disposition properties of 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol demonstrate their toxicological relevance and consequently the possible need to establish a tolerable daily intake.
Nathan
N
Broekaert
Mathias
M
Devreese
Thomas
T
De Mil
Sophie
S
Fraeyman
Department of Pathology, Bacteriology and Poultry Diseases, Laboratory of Bacteriology and Mycology, Faculty of Veterinary Medicine, Ghent University , Salisburylaan 133, 9820 Merelbeke, Belgium.
Gunther
G
Antonissen
Siegrid
S
De Baere
Patrick
P
De Backer
Department of Bioanalysis, Laboratory of Medical Biochemistry and Clinical Analysis, Faculty of Pharmaceutical Sciences, Ghent University , Ottergemsesteenweg 460, 9000 Gent, Belgium.
An
A
Vermeulen
Siska
S
Croubels
D000284:Administration, Oral; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D006868:Hydrolysis; D009183:Mycotoxins / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*; D013552:Swine / Q000378:metabolism*; D066007:Toxicokinetics; D014255:Trichothecenes / Q000737:chemistry / Q000493:pharmacokinetics* / Q000633:toxicity
D009183:Mycotoxins; D014255:Trichothecenes; C043247:3-acetyldeoxynivalenol; C046760:15-acetyldeoxynivalenol; C007262:deoxynivalenol
15-acetyldeoxynivalenol (15ADON); 3-acetyldeoxynivalenol (3ADON); broiler chicken; deoxynivalenol (DON); in vivo hydrolysis; pig; toxicokinetics