Interleukin-1β promotes the neurogenesis of carotid bodies by stimulating the activation of ERK1/2.
The carotid body (CB) is a complex sensory organ that functions to sense homeostatic O2 in the blood. Previous studies have shown that CBs express interleukin (IL)-1 receptor type I and that the chemosensitivity of CBs is increased following stimulation with pro-inflammatory cytokines. However, the effects of pro-inflammatory cytokines, such as IL-1β, on the neurogenesis of CB are unclear. Thus, in this study, we aimed to assess the effects of IL-1β and intermittent hypobaric hypoxia (IHH) plus IL-1β on the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2, tyrosine hydroxylase (TH) and the expression of nestin, a well-established stem cell marker in the nervous system. The results showed that TH, nestin expression and ERK1/2 phosphorylation were increased in the rat CB following intraperitoneal injection of IL-1β. Moreover, IL-1β had additive effects on IHH. These results suggested that the plasticity of CB was increased following treatment with IL-1β and that ERK1/2 may be involved in neurogenic signaling in CBs.
Department of Psychiatry, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China; Department of Toxicology, Shaanxi Key Lab of Free Radical Biology and Medicine, The Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.
Fen
F
Xue
Institute of Neuroscience, Fourth Military Medical University, Xi'an 710032, China.
Ling
L
Liu
College of Life Science, Shaanxi Normal University, China.
Juan
J
Fan
Department of Psychiatry, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
ShanShan
S
He
Department of Psychiatry, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Rui
R
Li
Department of Psychiatry, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China; Department of Toxicology, Shaanxi Key Lab of Free Radical Biology and Medicine, The Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China. Electronic address: pengzhengwu1446@163.com.
Zheng-Wu
ZW
Peng
Institute of Neuroscience, Fourth Military Medical University, Xi'an 710032, China. Electronic address: wbrnim@fmmu.edu.cn.
Bai-Ren
BR
Wang
D000818:Animals; D015153:Blotting, Western; D002344:Carotid Body / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D000860:Hypoxia / Q000503:physiopathology; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D053583:Interleukin-1beta / Q000008:administration & dosage / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008297:Male; D051379:Mice; D064231:Nestin / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology*; D010766:Phosphorylation; D010784:Photomicrography; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein; C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D064231:Nestin; D011994:Recombinant Proteins; D014446:Tyrosine 3-Monooxygenase
Carotid body; Extracellular signal-regulated kinase 1/2; Interleukin-1β; Nestin