Localization and role of RAP55/LSM14 in HeLa cells: a new finding on the mitotic spindle assembly.
The MAP family includes large proteins like MAP-1A, MAP-1B, MAP-1C, MAP-2, and MAP-4 and smaller components like tau and MAP-2C. This article focuses on the relevant aspects of RAP55/LSM14 position with emphasis on its role in mitotic spindle formation and stability. In this context, the localization of RNA associated Protein 55kDa (RAP55/LSM14) during mitosis was identified as a Mitotic Spindle Protein (MSP). We found a new location obtained by expressing GFP-tagged proteins in HeLa Cells during mitosis that has never been previously reported. We demonstrated also, for the first time, that the depletion of RAP55/LSM14 destabilizes spindle assembly, stops cells in mitosis and induces many other cell cytoskeletal disorders. Finally, by using an "in vitro" assay investigation, we found that RAP55/LSM14 binds directly the tubulin and that is implicated in the process of the mitotic spindle stabilization, which is a novel discovery in this field of research.
Inserm Unité 836, 38043 Grenoble, France; UR 12ES08, Signalisation Cellulaire et Pathologies, Faculté de Médecine Monastir, Université de Monastir, Tunisie.
Donia
D
Mili
Inserm Unité 836, 38043 Grenoble, France.
Dane
D
Georgesse
UR 12ES08, Signalisation Cellulaire et Pathologies, Faculté de Médecine Monastir, Université de Monastir, Tunisie.
Abderraouf
A
Kenani
D045744:Cell Line, Tumor; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis; D010641:Phenotype; D034741:RNA, Small Interfering / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
D034741:RNA, Small Interfering; C510649:RNA-associated protein 55, human; D012261:Ribonucleoproteins; D014404:Tubulin; D049452:Green Fluorescent Proteins