Functionalized, Fluorescent, Conjugated Polymer Nanospheres for Protein Targeting via Förster Resonance Energy Transfer.
We report the preparation of fluorescent nanospheres based on conjugated polymers, which enables a facile fluorescence color tuning. The fluorescent nanospheres have aldehyde groups on the surface that enable the introduction of a protein ligand, biotin. The intrinsic fluorescence of the nanospheres allows detection of a dye-labeled target protein, streptavidin, via Förster resonance energy transfer. The controlled biofunctionalization of conjugated polymer-based fluorescent nanospheres represents a novel approach with high applicability to sensing of biological molecules.
Jaeguk
J
Noh
Geunseok
G
Jang
Jongho
J
Kim
Daigeun
D
Kim
Taek Seung
TS
Lee
D001710:Biotin / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D008422:Materials Testing; D015336:Molecular Probe Techniques; D058726:Nanoconjugates / Q000737:chemistry / Q000648:ultrastructure; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000032:analysis*; D013499:Surface Properties
D005456:Fluorescent Dyes; D058726:Nanoconjugates; D001710:Biotin; D019809:Streptavidin