Sealing ability of two root-end filling materials in a bacterial nutrient leakage model.
OBJECTIVE
To compare in vitro the sealing ability of root-end fillings with mineral trioxide aggregate (MTA) and EndoSequence BioCeramic Root Repair Material-Fast Set (BC-RRM) Putty using a novel bacterial nutrient leakage model, which provides information on whether or not intracanal bacteria are receiving nutrients from serum via leakage channels.


METHODS
Sixty single-rooted decoronated mandibular incisors with instrumented root canals were subjected to root-end resection and ultrasonic preparation. The root specimens were mounted in the experimental apparatus, and the root-end cavities filled with the test materials. The positive control group used warm Gutta-percha and no sealer. In the negative controls, the entire resected surface was covered with varnish. After sterilization in ethylene oxide, the root canal was inoculated with 1.5 × 10(5) washed cells of Enterococcus faecalis. The apparatus was filled with foetal bovine serum, leaving only the apical root immersed. After 30-day incubation, samples were taken from the canal, cultured and the colony-forming units (CFUs) counted. Statistical analysis was performed using the Mann-Whitney test for quantitative and the Fisher exact test for qualitative data.


RESULTS
In the MTA group, 10 of 20 (50%) specimens still had detectable viable bacteria in the canals (mean, 8.97 × 10(3)  CFUs). In the BC-RRM Putty group, 5 of 18 (28%) specimens were positive for bacterial growth (mean, 2.88 × 10(4)  CFUs). There was no significant difference when comparing the quantitative or presence/absence data from the MTA and BC-RRM Putty groups. Positive and negative controls yielded the expected results.


CONCLUSIONS
MTA and BC-RRM Putty had similar sealing ability. The experimental model was effective in determining whether or not residual intracanal bacteria could survive by receiving nutrients from outside.
Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.|Department of Endodontics, Faculty of Dentistry, Grande Rio University, Duque de Caxias, Brazil.
H S
HS
Antunes
Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.|Department of Endodontics, Federal University of Campina Grande, Campina Grande, Brazil.
L F
LF
Gominho
Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.|Department of Health, Dentistry Division, Southwest State University of Bahia, Jequié, Brazil.
C V
CV
Andrade-Junior
Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.|Department of Endodontics, Vila Velha University, Vila Velha, Brazil.
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N
Dessaune-Neto
Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil. flavioferreiraalves@gmail.com.
F R F
FR
Alves
Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.
I N
IN
Rôças
Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.
J F
JF
Siqueira
D017607:Aluminum Compounds; D017610:Calcium Compounds; D002130:Calcium Phosphates; D003763:Dental Leakage / Q000382:microbiology / Q000517:prevention & control*; D004338:Drug Combinations; D006171:Gutta-Percha; D006801:Humans; D008954:Models, Biological; D010087:Oxides; D010485:Periapical Periodontitis / Q000601:surgery; D012387:Root Canal Filling Materials; D012389:Root Canal Obturation / Q000379:methods*; D017640:Silicates
D017607:Aluminum Compounds; D017610:Calcium Compounds; D002130:Calcium Phosphates; D004338:Drug Combinations; C550603:EndoSequence root repair material; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D006171:Gutta-Percha
bioceramic material; calcium silicate cement; endodontic infection; periradicular surgery; root-end filling