Reducing adsorption to improve recovery and in vivo detection of neuropeptides by microdialysis with LC-MS.
Neuropeptides are an important class of neurochemicals; however, measuring their concentration in vivo by using microdialysis sampling is challenging due to their low concentration and the small samples generated. Capillary liquid chromatography with mass spectrometry (cLC-MS) can yield attomole limits of detection (LOD); however, low recovery and loss of sample to adsorptive surfaces can still hinder detection of neuropeptides. We have evaluated recovery during sampling and transfer to the cLC column for a selection of 10 neuropeptides. Adding acetonitrile to sample eliminated carryover and improved LOD by 1.4- to 60-fold. The amount of acetonitrile required was found to have an optimal value that correlated with peptide molecular weight and retention time on a reversed phase LC column. Treating AN69 dialysis membrane, which bears negative charge due to incorporated sulfonate groups, with polyethylenimine (PEI) improved recovery by 1.2- to 80-fold. The effect appeared to be due to reducing electrostatic interaction between peptides and the microdialysis probe because modification increased recovery only for peptides that carried net positive charge. The combined effects improved LOD of the entire method by 1.3- to 800-fold for the different peptides. We conclude that peptides with both charged and hydrophobic regions require combined strategies to prevent adsorption and yield the best possible detection. The method was demonstrated by determining orexin A, orexin B, and a novel isoform of rat β-endorphin in the arcuate nucleus. Dialysate concentrations were below 10 pM for these peptides. A standard addition study on dialysates revealed that while some peptides can be accurately quantified, some are affected by the matrix.
Department of Chemistry, University of Michigan , Ann Arbor, Michigan 48109-1055, United States.
Ying
Y
Zhou
Department of Chemistry, University of Michigan , Ann Arbor, Michigan 48109-1055, United States.
Jenny-Marie T
JM
Wong
Department of Chemistry, University of Michigan , Ann Arbor, Michigan 48109-1055, United States.
Omar S
OS
Mabrouk
Department of Chemistry, University of Michigan , Ann Arbor, Michigan 48109-1055, United States.
Robert T
RT
Kennedy
D000327:Adsorption; D000818:Animals; D001923:Brain Chemistry; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D002853:Chromatography, Liquid; D004867:Equipment Design; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods*; D009479:Neuropeptides / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D009479:Neuropeptides