Iron nanoparticles decorated multi-wall carbon nanotubes modified carbon paste electrode as an electrochemical sensor for the simultaneous determination of uric acid in the presence of ascorbic acid, dopamine and L-tyrosine.
Iron nanoparticles decorated multi-wall carbon nanotubes modified carbon paste electrode (Fe-MWCNTs/MCPE) was prepared by bulk-modification method. The electrochemical impedance spectroscopy (EIS) suggests least charge transfer resistance at the modified electrode. The electrochemical behavior of UA was studied in 0.1M phosphate buffer solution (PBS) of pH3.0 using cyclic voltammetry (CV) while differential pulse voltammetry (DPV) was used for quantification. The spectroelectrochemial study of oxidation of UA at Fe-MWCNTs/MCPE showed a decrease in the absorbance of two peaks with time, which are ascribed to π to π(⁎) and n to π(⁎) transitions. Under optimum condition, the DPV response offered two linear dynamic ranges for UA in the concentration range 7.0×10(-8)M-1.0×10(-6)M and 2.0×10(-6)M-1.0×10(-5)M with detection limit (4.80±0.35)×10(-8)M (S/N=3). The practical analytical application of this sensor was successfully evaluated by determination of spiked UA in clinical samples, such as human blood serum and urine with good percentage recovery. The proposed electrochemical sensor offers a simple, reliable, rapid, reproducible and cost effective analysis of a quaternary mixture of biomolecules containing AA, DA, UA and Tyr which was free from mutual interferences.
Department of Postgraduate Studies and Research in Chemistry, St. Joseph's College (Autonomous), Lalbagh Road, Bangalore 560 027, Karnataka, India.
Arvind K
AK
Bhakta
Electrochemistry Research Group, Department of Chemistry, St. Joseph's College, Lalbagh Road, Bangalore 560 027, Karnataka, India. Electronic address: ronaldmasc2311@yahoo.co.in.
Ronald J
RJ
Mascarenhas
Electrochemistry Research Group, Department of Chemistry, St. Joseph's College, Lalbagh Road, Bangalore 560 027, Karnataka, India.
Ozma J
OJ
D'Souza
Department of Analytical Chemistry, Bhabha Atomic Research Centre, Anushakthi Nagar, Trombay, Mumbai 400 094, Maharashtra, India.
Ashis K
AK
Satpati
Laboratoire de Chimie et d'Electrochimie des Surface, University of Namur, 61 Rue de Bruxelles, B-5000 Namur, Belgium.
Simon
S
Detriche
Laboratoire de Chimie et d'Electrochimie des Surface, University of Namur, 61 Rue de Bruxelles, B-5000 Namur, Belgium.
Zineb
Z
Mekhalif
Laboratoire de Chimie et d'Electrochimie des Surface, University of Namur, 61 Rue de Bruxelles, B-5000 Namur, Belgium.
Joseph
J
Dalhalle
D001205:Ascorbic Acid / Q000097:blood / Q000652:urine; D015374:Biosensing Techniques / Q000295:instrumentation; D002244:Carbon / Q000737:chemistry; D045424:Complex Mixtures / Q000097:blood / Q000652:urine; D003217:Conductometry / Q000295:instrumentation*; D004298:Dopamine / Q000097:blood / Q000652:urine; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007501:Iron / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008839:Microelectrodes; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009824:Ointments; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014443:Tyrosine / Q000097:blood / Q000652:urine; D014527:Uric Acid / Q000097:blood* / Q000652:urine*
D045424:Complex Mixtures; D037742:Nanotubes, Carbon; D009824:Ointments; D014527:Uric Acid; D014443:Tyrosine; D002244:Carbon; D007501:Iron; D001205:Ascorbic Acid; D004298:Dopamine
Ascorbic acid; Differential pulse voltammetry; Dopamine; Iron nanoparticles decorated multi-wall carbon nanotubes; Uric acid; l-Tyrosine