CD133 antibody conjugation to decellularized human heart valves intended for circulating cell capture.
The long term efficacy of tissue based heart valve grafts may be limited by progressive degeneration characterized by immune mediated inflammation and calcification. To avoid this degeneration, decellularized heart valves with functionalized surfaces capable of rapid in vivo endothelialization have been developed. The aim of this study is to examine the capacity of CD133 antibody-conjugated valve tissue to capture circulating endothelial progenitor cells (EPCs). Decellularized human pulmonary valve tissue was conjugated with CD133 antibody at varying concentrations and exposed to CD133 expressing NTERA-2 cl.D1 (NT2) cells in a microflow chamber. The amount of CD133 antibody conjugated on the valve tissue surface and the number of NT2 cells captured in the presence of shear stress was measured. Both the amount of CD133 antibody conjugated to the valve leaflet surface and the number of adherent NT2 cells increased as the concentration of CD133 antibody present in the surface immobilization procedure increased. The data presented in this study support the hypothesis that the rate of CD133(+) cell adhesion in the presence of shear stress to decellularized heart valve tissue functionalized by CD133 antibody conjugation increases as the quantity of CD133 antibody conjugated to the tissue surface increases.
Wake Forest Institute for Regenerative Medicine and, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA. Department of General Surgery, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
John D
JD
Vossler
Young
Y
Min Ju
J Koudy
JK
Williams
Steven
S
Goldstein
James
J
Hamlin
Sang Jin
SJ
Lee
James J
JJ
Yoo
Anthony
A
Atala
D000071916:AC133 Antigen; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D001705:Bioprosthesis; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D002474:Cell-Free System / Q000276:immunology / Q000473:pathology; D066026:Endothelial Progenitor Cells / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D006350:Heart Valve Prosthesis; D006801:Humans; D010455:Peptides / Q000276:immunology*; D011664:Pulmonary Valve / Q000166:cytology* / Q000276:immunology
D000071916:AC133 Antigen; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides