A disposable sampling device to collect volume-measured DBS directly from a fingerprick onto DBS paper.
BACKGROUND
DBS samples collected from a fingerprick typically vary in volume and homogeneity and hence make an accurate quantitative analysis of DBS samples difficult.


RESULTS
We report a prototype which first defines a precise liquid volume and subsequently stores it to a conventional DBS matrix. Liquid volumes of 2.2 µl ± 7.1% (n = 21) for deionized water and 6.1 µl ± 8.8% (n = 15) for whole blood have been successfully metered and stored in DBS paper.


CONCLUSIONS
The new method of collecting a defined volume of blood by DBS sampling has the potential to reduce assay bias for the quantitative evaluation of DBS samples while maintaining the simplicity of conventional DBS sampling.
Department for Micro & Nanosystems, KTH Royal Institute of Technology, Osquldas väg 10, 10044 Stockholm, Sweden.
Gabriel
G
Lenk
Department of Clinical Pharmacology, Karolinska University Hospital, 14152 Stockholm, Sweden.
Sören
S
Sandkvist
Department of Clinical Pharmacology, Karolinska University Hospital, 14152 Stockholm, Sweden.
Anton
A
Pohanka
Department for Micro & Nanosystems, KTH Royal Institute of Technology, Osquldas väg 10, 10044 Stockholm, Sweden.
Göran
G
Stemme
Department of Clinical Pharmacology, Karolinska University Hospital, 14152 Stockholm, Sweden.
Olof
O
Beck
Department for Micro & Nanosystems, KTH Royal Institute of Technology, Osquldas väg 10, 10044 Stockholm, Sweden.
Niclas
N
Roxhed
D000661:Amphetamine / Q000097:blood; D001800:Blood Specimen Collection / Q000295:instrumentation* / Q000379:methods; D004209:Disposable Equipment; D059788:Dried Blood Spot Testing / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005385:Fingers; D006801:Humans; D056656:Lab-On-A-Chip Devices; D010209:Paper; D012996:Solutions / Q000737:chemistry
D012996:Solutions; D000661:Amphetamine