[Increased manganese superoxide dismutase and cyclin B1 expression in carnosine-induced inhibition of glioblastoma cell proliferation].
Carnosine is an endogenous dipeptide with antiproliferative properties. Here we show that carnosine selectively inhibits proliferation of human glioblastoma cells (U-118-MG) compared to breast (MB231) and oral (Cal27 and FaDu) cancer cells. Carnosine-induced inhibition of U-118-MG proliferation is associated with a significant: decrease in cellular reactive oxygen species levels, increase in manganese superoxide dismutase (MnSOD) and increase in cyclin B1 expression resulting in G2-block. We conclude that the antiproliferative property of carnosine is due to its ability to enhance MnSOD and cyclin B1 expression. These results will be of significance to the potential application of carnosine in brain cancer therapy.
Neurology Research Center, Moscow, Russia.
Yu S
YS
Rybakova
Free Radical and Radiation Biology Division, Department of Radiation Oncology, University of Iowa, Iowa City, USA.
A L
AL
Kalen
Free Radical and Radiation Biology Division, Department of Radiation Oncology, University of Iowa, Iowa City, USA.
J C
JC
Eckers
Neurology Research Center, Moscow, Russia.
T N
TN
Fedorova
Free Radical and Radiation Biology Division, Department of Radiation Oncology, University of Iowa, Iowa City, USA.
P C
PC
Goswami
Free Radical and Radiation Biology Division, Department of Radiation Oncology, University of Iowa, Iowa City, USA.
E H
EH
Sarsour
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002336:Carnosine / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D056744:Cyclin B1 / Q000819:agonists / Q000235:genetics* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009928:Organ Specificity; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism
D000970:Antineoplastic Agents; D056744:Cyclin B1; D017382:Reactive Oxygen Species; D002336:Carnosine; D013482:Superoxide Dismutase
G2-block; MnSOD; anserine; carnosine; cell cycle; cyclin B1