Proteomic Changes Associated with Successive Reproductive Periods in Male Polychaetous Neanthes arenaceodentata.
The polychaetous annelid Neanthes acuminata complex has a widespread distribution, with the California population referred to as N. arenaceodentata. The reproductive pattern in this complex is unique, in that the female reproduces once and then dies, whereas the male can reproduce up to nine times. The male incubates the embryos until the larvae leave the male's tube 21-28 days later and commences feeding. Reproductive success and protein expression patterns were measured over the nine reproductive periods. The percent success of the male in producing juveniles increased during the first three reproductive periods and then decreased, but the number of juveniles produced was similar through all nine periods. iTRAQ based quantitative proteomics were used to analyze the dynamics of protein expression patterns. The expression patterns of several proteins were found to be altered. The abundant expression of muscular and contractile proteins may have affected body weight and reproductive success. Sperm have never been observed; fertilization occurs within the parent's tube. Proteins associated with sperm maturation and fertilization were identified, including ATPase, clathrin, peroxiredoxins and enolase, which may provide clues to the molecular mechanisms enabling males to reproduce multiple times.
KAUST Environmental Epigenetic Program (KEEP), Division of Biological and Environmental Sciences &Engineering, Division of Applied Mathematics and Computer Sciences. King Abdullah University of Science and Technology, Kingdom of Saudi Arabia.
Kondethimmanahalli H
KH
Chandramouli
Department of Biological Sciences, California State University, Long Beach, California, United States.
Donald
D
Reish
Bioscience Core Laboratory, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia.
Huoming
H
Zhang
KAUST Global Collaborative Research Program, Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong.
Pei-Yuan
PY
Qian
KAUST Environmental Epigenetic Program (KEEP), Division of Biological and Environmental Sciences &Engineering, Division of Applied Mathematics and Computer Sciences. King Abdullah University of Science and Technology, Kingdom of Saudi Arabia.
Timothy
T
Ravasi
D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology; D007814:Larva; D008297:Male; D011077:Polychaeta / Q000502:physiology*; D020543:Proteome / Q000378:metabolism*; D012098:Reproduction / Q000502:physiology*; D012737:Sex Factors
D020543:Proteome