Opposite Effects of Lysophosphatidylethanolamines on Conformation of OmpF-like Porin from Yersinia pseudotuberculosis.
Lysophosphatidyletnolamine (LPE) is one of enigmatic lipids of bacteria. It is generated from major membrane lipid - phosphatidylethanolamine at severe changes of the bacterial growth conditions. Accumulation of this phospholipid in cells of Gram-negative enterobacterium Yersinia pseudotuberculosis results in the enhanced thermostability of OmpF-like porin (YOmpF) from the same bacteria. The respective integral conformational rearrangements may disturb the channel permeability of protein under stress conditions. However, role of fatty acid composition of LPE in this effect remained unclear. Present work demonstrated that the level of unsaturated LPE is 3.5 times higher than saturated one in total LPE of bacterial cells exposed to stress (phenol treatment). Unsaturated 1-oleoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine (MOPE) and saturated LPE 1-palmitoyl-2- hydroxy-sn-glycero-3-phosphoethanolamine (MPPE) oppositely affect the conformation of YOmpF. MOPE increases the protein thermal stability due to more dense packing of monomers in porin and preserves its trimeric form at elevated temperature, while MPPE weakens the contact between monomers and promotes dissociation of the protein.
Ludmila A
LA
Davydova
Department of Biochemistry, Microbiology and Biotechnology, School of Natural Sciences, Far Eastern Federal University, 690950, Vladivostok, Russia. sanina.nm@dvfu.ru.
Nina M
NM
Sanina
Olga D
OD
Novikova
Olga Y
OY
Portnyagina
Svetlana I
SI
Bakholdina
Peter V
PV
Velansky
Natalia S
NS
Vorobyeva
Valentina A
VA
Khomenko
Valery L
VL
Shnyrov
Mikhail V
MV
Bogdanov
D001426:Bacterial Proteins / Q000737:chemistry* / Q000187:drug effects*; D015153:Blotting, Western; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D008246:Lysophospholipids / Q000494:pharmacology*; D018272:Porins / Q000737:chemistry* / Q000187:drug effects*; D011487:Protein Conformation / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D015011:Yersinia pseudotuberculosis / Q000737:chemistry* / Q000235:genetics
D001426:Bacterial Proteins; D005227:Fatty Acids; D008246:Lysophospholipids; C109237:OmpF protein; D018272:Porins; C008301:lysophosphatidylethanolamine