Identification and functional characterization of TRPA1 in human myoblasts.
The proper function of the skeletal muscle is essential for the survival of most animals. Thus, efficient and rapid repair of muscular damage following injury is crucial. In recent years, satellite cells have emerged as key players of muscle repair, capable of undergoing extensive proliferation after injury, fusing into myotubes and restoring muscle function. Furthermore, it has been shown that Ca(2+)/calmodulin-dependent generation of nitric oxide (NO) is an important regulator of muscle repair. Here, we demonstrate the functional expression of transient receptor potential, subfamily A1 (TRPA1) channel in human primary myoblasts. Stimulation of these cells with well-known TRPA1 ligands led to robust intracellular Ca(2+) rises which could be inhibited by specific TRPA1 antagonists. Moreover, we show that TRPA1 activation enhances important aspects of skeletal muscle repair such as cell migration and myoblast fusion in vitro. Interestingly, TRPA1 levels and inducible Ca(2+) transients decline with ongoing myoblast differentiation. We suggest that TRPA1 might serve as a physiological mediator for inflammatory signals and appears to have a functional role in promoting myoblast migration, fusion, and potentially also in activating satellite cells in humans.
Ruhr University Bochum, Universitätsstr. 150, 44801, Bochum, Germany. markus.osterloh-2@rub.de.
Markus
M
Osterloh
Universities of Giessen and Marburg Lung Center, Aulweg 130, 35392, Giessen, Germany.
Mario
M
Böhm
Ruhr University Bochum, Universitätsstr. 150, 44801, Bochum, Germany.
Benjamin
B
Kalbe
Ruhr University Bochum, Universitätsstr. 150, 44801, Bochum, Germany.
Sabrina
S
Osterloh
Ruhr University Bochum, Universitätsstr. 150, 44801, Bochum, Germany.
Hanns
H
Hatt
D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D020013:Calcium Signaling; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D006801:Humans; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000074025:TRPA1 Cation Channel; D050051:Transient Receptor Potential Channels / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
D015220:Calcium Channels; D009419:Nerve Tissue Proteins; D000074025:TRPA1 Cation Channel; C473350:TRPA1 protein, human; D050051:Transient Receptor Potential Channels
Differentiation; Myoblasts; Nitric oxide; Skeletal muscle regeneration; Thymol; Transient receptor potential channel