Expression and significance of m1A transmethylase, hTrm6p/hTrm61p and its related gene hTrm6/hTrm61 in bladder urothelial carcinoma.
OBJECTIVE
To discuss the expression of hTrm6p/hTrm61p in bladder urothelial carcinoma tissue and its relationship with m1A level in urine, as well as the influences of hTrm6/hTrm61 on the proliferation and apoptosis of cancer cell line on urothelium.


METHODS
m1A levels in urine of 32 patients of bladder urothelial carcinoma and normal people were detected by HPLC/ESI-Q-TOF-MS, hTrm6p/hTrm61p expression levels in cancer tissue and para-carcinoma tissue of the same patient were detected by western blotting, and hTrm61 expressions of cancer cell line T24, 5637, and EJ of bladder urothelium and kidney cell line HEK-293 of human embryo were detected by RT-qPCR. The hTrm61 high-expression cell line is selected to detect the situation of proliferation and apoptosis by CCK-8 and flow cytometry (FCM) after knocking out hTrm61 with siRNA.


RESULTS
m1A level in urine of carcinoma of urinary bladder is significantly higher than that of normal people, and hTrm6p/hTrm61p expression level in cancer tissue is significantly higher than that in para-carcinoma tissue and has linear correlation with m1A level in urine. The hTrm61 expression in cell line 5637 is significantly higher than that of T24, EJ, and HEK293, and apoptosis is significantly affected after hTrm61 is knocked out from 5637 cell line.


CONCLUSIONS
High-level expression of hTrm6p/hTrm61p is an important reason for high emission of m1A in urine, and hTrm6/hTrm61 promotes the occurrence of carcinoma of urinary bladder.
Department of Urology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
Lei
L
Shi
Department of Urology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
Xiao-Ming
XM
Yang
Department of Urology, The Chinese People's Liberation Army 153 Central Hospital Zhengzhou 450000, China.
Dong-Dong
DD
Tang
Department of Intensive Medicine, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
Gang
G
Liu
Department of Intensive Medicine, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
Pu
P
Yuan
Department of Urology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
Yang
Y
Yang
Department of Urology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
Lian-Sheng
LS
Chang
Department of Pharmacology, School of Basic Medicine, Zhengzhou University Zhengzhou 450001, China.
Li-Rong
LR
Zhang
Department of Urology, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
Dong-Kui
DK
Song
Bladder urothelial carcinoma; HPLC/ESI-Q-TOF-MS; hTrm6/hTrm61; hTrm6p/hTrm61p; m1A; urinary modified nucleosides