Transcriptional networks associated with 5-alpha-dihydrotestosterone in the fathead minnow (Pimephales promelas) ovary.
Androgens play a significant role in regulating oogenesis in teleost fishes. The androgen dihydrotestosterone (DHT) is a potent non-aromatizable androgen involved in sexual differentiation in mammals; however, its actions are not well understood in teleost fish. To better characterize the physiological role of DHT in the fathead minnow (FHM) ovary on a temporal scale, in vitro assays for 17β-estradiol (E2) production were conducted in parallel with microarray analysis. Ovarian explants were incubated at different concentrations of DHT (10(-6), 10(-7), and 10(-8)M DHT) in three separate experiments conducted at 6, 9, and 12h. DHT treatment resulted in a rapid and consistent increase in E2 production from the ovary at all three time points. Therefore, DHT may act to shift the balance of metabolites in the steroidogenic pathway within the ovary. Major biological themes affected by DHT in the ovary in one or more of the time points included those related to blood (e.g. vasodilation, blood vessel contraction, clotting), lipids (e.g. lipid storage, cholesterol metabolism, lipid degradation) and reproduction (e.g. hormone and steroid metabolism). Gene networks related to immune responses and calcium signaling were also affected by DHT, suggesting that this androgen may play a role in regulating these processes in the ovary. This study detected no change in mRNA levels of steroidogenic enzymes (cyp19a1, star, 11βhsd, 17βhsd, srd5a isoforms), suggesting that the observed increase in E2 production is likely more dependent on the pre-existing gene or protein complement in the ovary rather than the de novo expression of transcripts. This study increases knowledge regarding the roles of DHT and androgens in general in the teleost ovary and identifies molecular signaling pathways that may be associated with increased E2 production.
Department of Biology and Canadian Rivers Institute, University of New Brunswick, Saint John, New Brunswick E2L 4L5, Canada.
Anna
A
Ornostay
Department of Biology and Canadian Rivers Institute, University of New Brunswick, Saint John, New Brunswick E2L 4L5, Canada.
Joshua
J
Marr
Department of Biology and Canadian Rivers Institute, University of New Brunswick, Saint John, New Brunswick E2L 4L5, Canada.
Jennifer R
JR
Loughery
Department of Biology and Canadian Rivers Institute, University of New Brunswick, Saint John, New Brunswick E2L 4L5, Canada; Department of Physiological Sciences and Center for Environmental and Human Toxicology, UF Genetics Institute, University of Florida, Gainesville, FL 32611, USA. Electronic address: cmartyn@ufl.edu.
Christopher J
CJ
Martyniuk
D000818:Animals; D003530:Cyprinidae / Q000235:genetics / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism / Q000494:pharmacology*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D053263:Gene Regulatory Networks; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D012098:Reproduction / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics
D013196:Dihydrotestosterone; D004958:Estradiol
Androgens; Fish reproduction; Gene regulatory networks; Lipid metabolism; Steroids; Sub-network enrichment analysis