In vivo metabolite identification of bakuchiol in rats by UPLC/ESI-PDA-QTOF-MS.
Psoralea corylifolia L. is a traditional Chinese medicine for the treatment of various skin diseases such as psoriasis, vitiligo and chronic graft-versus-host. Bakuchiol, isolated from the seeds of P. corylifolia L., is one of the most important active compounds. To study the metabolic fate of bakuchiol in rats, an ultra performance liquid chromatography/electrospray ionization-photo diode array-quadrupole time of flight-mass spectrometry (UPLC/ESI-PDA-QTOF-MS) combined with MetaboLynx XS software was used, peak area-time curves of major metabolites in plasma from bakuchiol were determined. A total of 11 metabolites were identified after a single oral administration of bakuchiol, including 6 in plasma, 10 in bile, 8 in urine and 2 in feces, the metabolic transformation pathways of bakuchiol in rats included oxidation, hydroxylation, methylation, O-glucuronide conjugation and O-sulfate conjugation. In conclusion, this study expands our knowledge about the metabolism of bakuchiol which will be conducive to reveal its in vivo pharmacological and toxicological material basis, in addition, UPLC/ESI-PDA-QTOF-MS coupled with MetaboLynx XS software can be adopted as a useful tool for quick detection and identification of metabolites from medicine in vivo.
College of Pharmacy, South Central University for Nationalities, Hubei, Wuhan 430000, China; Beijing Institute of Radiation Medicine, Beijing 100850, China.
Chao
C
Hu
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Qiande
Q
Liang
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Xianglin
X
Tang
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Yuguang
Y
Wang
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Zengchun
Z
Ma
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Chengrong
C
Xiao
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Hongling
H
Tan
Beijing Institute of Radiation Medicine, Beijing 100850, China. Electronic address: gaoyue@bmi.ac.cn.
Yue
Y
Gao
College of Pharmacy, South Central University for Nationalities, Hubei, Wuhan 430000, China. Electronic address: huangxianju1972@yahoo.cn.
Xianju
X
Huang
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000737:chemistry; D008297:Male; D053858:Metabolic Networks and Pathways; D015394:Molecular Structure; D010636:Phenols / Q000378:metabolism* / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D010636:Phenols; C012765:bakuchiol
Bakuchiol; Identification; Metabolite profile; Rat; UPLC/ESI–PDA–QTOF–MS