Ultra fast magic angle spinning solid - state NMR spectroscopy of intact bone.
Ultra fast magic angle spinning (MAS) has been a potent method to significantly average out homogeneous/inhomogeneous line broadening in solid-state nuclear magnetic resonance (ssNMR) spectroscopy. It has given a new direction to ssNMR spectroscopy with its different applications. We present here the first and foremost application of ultra fast MAS (~60 kHz) for ssNMR spectroscopy of intact bone. This methodology helps to comprehend and elucidate the organic content in the intact bone matrix with resolution and sensitivity enhancement. At this MAS speed, amino protons from organic part of intact bone start to appear in (1) H NMR spectra. The experimental protocol of ultra-high speed MAS for intact bone has been entailed with an additional insight achieved at 60 kHz.
Centre of Biomedical Research, SGPGIMS Campus, Raebarelly Road, Lucknow, UP, 226014, India.|School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India.
Chandan
C
Singh
Centre of Biomedical Research, SGPGIMS Campus, Raebarelly Road, Lucknow, UP, 226014, India.
Ratan Kumar
RK
Rai
School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India.
Arvind M
AM
Kayastha
Centre of Biomedical Research, SGPGIMS Campus, Raebarelly Road, Lucknow, UP, 226014, India.
Neeraj
N
Sinha
D000818:Animals; D001857:Bone Matrix / Q000737:chemistry*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D003094:Collagen / Q000737:chemistry; D006041:Goats; D066244:Proton Magnetic Resonance Spectroscopy
D003094:Collagen
1H; 1H-13C; MAS; NH protons; bone; sensitivity enhancement; ssNMR; ultra fast magic angle spinning; water