Isolation and Characterization of an Unknown Chrysosporium sp. Producing Subcutaneous Mycosis in an Immunocompromised Patient.
We report a case of subcutaneous fungal abscess over the great toe caused by a keratinophilic fungus, an unknown Chrysosporium sp., in a 60-year-old diabetic female who was treated successfully with oral fluconazole. The fungus was isolated from aspirated pus, and septate hyphae were seen in fine needle aspiration cytology. Ovoid- to club-shaped hyaline one-celled conidia (aleuriconidia) with broad truncated bases were seen, and sequencing of ITS1-5.8S-ITS2 rDNA revealed belonging to the order Onygenales and most closely related to Chrysosporium spp. isolated from a fowl. Of the 65 species within the genus Chrysosporium, very few have been reported as pathogenic.
Department of Microbiology, Father Muller Medical College, Mangalore, 575002, India. sagar88doc@gmail.com.
Sagar
S
Chandrakar
Department of Microbiology, Father Muller Medical College, Mangalore, 575002, India.
Meena
M
Dias
Department of Surgery, Father Muller Medical College, Mangalore, India.
Prathvi
P
Shetty
Department of Microbiology, Father Muller Medical College, Mangalore, 575002, India.
Jutang Babat Ain
JB
Tiewsoh
Mycology Unit, School of Medicine and Health Sciences, Reus, Catalonia, Spain.
Josep
J
Guarro
D000038:Abscess / Q000209:etiology* / Q000382:microbiology / Q000473:pathology*; D000935:Antifungal Agents / Q000008:administration & dosage; D044963:Biopsy, Fine-Needle; D002912:Chrysosporium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D003881:Dermatomycoses / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology*; D048909:Diabetes Complications; D005260:Female; D015725:Fluconazole / Q000008:administration & dosage; D006801:Humans; D016867:Immunocompromised Host; D008828:Microbiological Techniques; D008875:Middle Aged; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D016896:Treatment Outcome
D000935:Antifungal Agents; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D015725:Fluconazole
Chrysosporium sp.; DNA sequencing; Keratinophilic; Subcutaneous