Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma.
BACKGROUND
Here, phytochemical profile of Nitraria retusa (N. Retusa) leaf extracts was identified and their ability to induce apoptosis and inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma was evaluated.


METHODS
The Apoptosis was evidenced by investigating DNA fragmentation, and Acridine orange/ethidium bromide staining. Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm.


RESULTS
Extracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation. Our result was confirmed by ladder DNA fragmentation profile. All extracts showed also the ability to enhance melanogenesis and tyrosinase activity of B16F10 melanoma cells.


CONCLUSIONS
The tested extracts have a significant biological effect which may be due to their bioactive compounds.
Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia. jihed.boubaker@yahoo.fr.|Unity of bioactive natural substances and biotechnology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia. jihed.boubaker@yahoo.fr.|Higher Institute of Medical Technologies of Tunis, Tunis El Manar University, Tunis El Manar, 2092, Tunisia. jihed.boubaker@yahoo.fr.
Jihed
J
Boubaker
Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia.|Unity of bioactive natural substances and biotechnology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia.
Imen
I
Mokdad Bzeouich
Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia.|Unity of bioactive natural substances and biotechnology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia.
Nouha
N
Nasr
Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia.|Unity of bioactive natural substances and biotechnology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia.
Hajer
H
Ben Ghozlen
Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia.|Unity of bioactive natural substances and biotechnology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia.
Nadia
N
Mustapha
Unity of bioactive natural substances and biotechnology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia.
Kamel
K
Ghedira
Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia.
Leila
L
Chekir-Ghedira
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D019684:Magnoliopsida / Q000737:chemistry*; D008546:Melanoma, Experimental / Q000378:metabolism*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
D000970:Antineoplastic Agents; D010936:Plant Extracts