Maternal flaxseed diet during lactation changes adrenal function in adult male rat offspring.
Flaxseed (Linum usitatissimum L.) has been a focus of interest in the field of functional foods because of its potential health benefits. However, we hypothesised that maternal flaxseed intake during lactation could induce several metabolic dysfunctions in adult offspring. In the present study, we aimed to characterise the adrenal function of adult offspring whose dams were supplemented with whole flaxseed during lactation. At birth, lactating Wistar rats were divided into two groups: rats from dams fed the flaxseed diet (FLAX) with 25% of flaxseed and controls dams. Pups received standard diet after weaning and male offspring were killed at age 180 days old to collect blood and tissues. We evaluated body weight and food intake during development, corticosteronaemia, adrenal catecholamine content, hepatic cholesterol, TAG and glycogen contents, and the protein expression of corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) and adrenaline β2 receptor at postnatal day 180 (PN180). After weaning, pups from the FLAX group had a higher body weight (+10 %) and food intake (+10%). At PN180, the FLAX offspring exhibited higher serum corticosterone (+48%) and lower adrenal catecholamine ( - 23%) contents, lower glycogen ( - 30%), higher cholesterol (4-fold increase) and TAG (3-fold-increase) contents in the liver, and higher 11β-HSD1 (+62%) protein expression. Although the protein expression of hypothalamic CRH was unaffected, the FLAX offspring had lower protein expression of pituitary ACTH ( - 34%). Therefore, induction of hypercorticosteronaemia by dietary flaxseed during lactation may be due to an increased hepatic activation of 11β-HSD1 and suppression of ACTH. The changes in the liver fat content of the FLAX group are suggestive of steatosis, in which hypercorticosteronaemia may play an important role. Thus, it is recommended that lactating women restrict the intake of flaxseed during lactation.
Laboratory of Endocrine Physiology, Department of Physiological Sciences, Biology Institute, State University of Rio de Janeiro,5o andar, Avenida 28 de setembro, 87,Rio de Janeiro,RJ,Brazil.
Mariana Sarto
MS
Figueiredo
Laboratory of Endocrine Physiology, Department of Physiological Sciences, Biology Institute, State University of Rio de Janeiro,5o andar, Avenida 28 de setembro, 87,Rio de Janeiro,RJ,Brazil.
Ellen Paula Santos
EP
da Conceição
Laboratory of Endocrine Physiology, Department of Physiological Sciences, Biology Institute, State University of Rio de Janeiro,5o andar, Avenida 28 de setembro, 87,Rio de Janeiro,RJ,Brazil.
Elaine
E
de Oliveira
Laboratory of Endocrine Physiology, Department of Physiological Sciences, Biology Institute, State University of Rio de Janeiro,5o andar, Avenida 28 de setembro, 87,Rio de Janeiro,RJ,Brazil.
Patricia Cristina
PC
Lisboa
Laboratory of Endocrine Physiology, Department of Physiological Sciences, Biology Institute, State University of Rio de Janeiro,5o andar, Avenida 28 de setembro, 87,Rio de Janeiro,RJ,Brazil.
Egberto Gaspar
EG
de Moura
D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000097:blood; D000311:Adrenal Glands / Q000503:physiopathology*; D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D001835:Body Weight; D002395:Catecholamines / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D003345:Corticosterone / Q000097:blood; D004032:Diet / Q000662:veterinary; D005260:Female; D019597:Flax / Q000009:adverse effects*; D006003:Glycogen / Q000097:blood; D007774:Lactation; D008099:Liver / Q000378:metabolism; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena; D023421:Models, Animal; D009752:Nutritional Status; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood; D014886:Weaning
D002395:Catecholamines; D014280:Triglycerides; D000324:Adrenocorticotropic Hormone; D006003:Glycogen; D002784:Cholesterol; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; D003345:Corticosterone
Adrenal function; Flaxseed; Lactation; Programming