Chemical Composition, Antioxidative and Anticancer Activities of the Essential Oil: Curcumae Rhizoma-Sparganii Rhizoma, a Traditional Herb Pair.
As a classical herb pair in clinics of traditional Chinese medicine, Curcumae Rhizoma-Sparganii Rhizoma (HP CR-SR) is used for activating blood circulation to remove blood stasis. The essential components in HP CR-SR and its single herbs were comparatively analyzed using gas chromatography-mass spectrometry data. 66, 22, and 54 components in volatile oils of Curcumae Rhizoma, Sparganii Rhizoma, and HP CR-SR were identified, and total contents accounted for 75.416%, 91.857%, and 79.553% respectively. The thirty-eight components were found in HP CR-SR, and not detected in single herbs Curcumae Rhizoma and Sparganii Rhizoma. The highest radical trapping action was seen by an essential oil of HP CR-SR (IC50 = 0.59 ± 0.04 mg/mL). Furthermore, the HP CR-SR essential oil showed more remarkable cytotoxicity on tumor cell lines than that of the single herbs Curcumae Rhizoma and Sparganii Rhizoma in a dose-dependent manner: IC50 values showing 32.32 ± 5.31 μg/mL (HeLa), 34.76 ± 1.82 μg/mL (BGC823), 74.84 ± 1.66 μg/mL (MCF-7), 66.12 ± 11.23 μg/mL (SKOV3), and 708.24 ± 943.91 μg/mL (A549), respectively. In summary, the essential oil of HP CR-SR is different from any one of Curcumae Rhizoma and Sparganii Rhizoma, nor simply their superposition, and HP CR-SR oil presented more remarkable anticancer and antioxidant activities compared with Curcumae Rhizoma and Sparganii Rhizoma oils.
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. xuguanling4004@163.com.
Guan-Ling
GL
Xu
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. piece124@sohu.com.
Di
D
Geng
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. xiemeng0819@163.com.
Meng
M
Xie
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. teng1995815@163.com.
Kai-Yue
KY
Teng
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. tianyuxin1216@163.com.
Yu-Xin
YX
Tian
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. lzz332@126.com.
Zi-Zhen
ZZ
Liu
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. 13269650940@163.com.
Cheng
C
Yan
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. 18739908461@163.com.
Yan
Y
Wang
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. zhangxia0561@126.com.
Xia
X
Zhang
Pharmacy College, Ningxia Medical University, Ningxia 750021, China. songyan200714@163.com.
Yan
Y
Song
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. 18311242015@163.com.
Yue
Y
Yang
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. shegaimei@126.com.
Gai-Mei
GM
She
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D030024:Curcuma / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D027343:Rhizome / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile
Curcumae Rhizoma; Curcumae Rhizoma–Sparganii Rhizoma; Sparganii Rhizoma; anti-cancer activity; antioxidant activity; essential oils; herb pair