Microwave-Induced Structural and Functional Injury of Hippocampal and PC12 Cells Is Accompanied by Abnormal Changes in the NMDAR-PSD95-CaMKII Pathway.
Recent studies have highlighted the important role of the postsynaptic NMDAR-PSD95-CaMKII pathway for synaptic transmission and related neuronal injury. Here, we tested changes in the components of this pathway upon microwave-induced neuronal structure and function impairments. Ultrastructural and functional changes were induced in hippocampal neurons of rats and in PC12 cells exposed to microwave radiation. We detected abnormal protein and mRNA expression, as well as posttranslational modifications in the NMDAR-PSD95-CaMKII pathway and its associated components, such as synapsin I, following microwave radiation exposure of rats and PC12 cells. Thus, microwave radiation may induce neuronal injury via changes in the molecular organization of postsynaptic density and modulation of the biochemical cascade that potentiates synaptic transmission.
Laboratory of Experimental Pathology, Beijing Institute of Radiation Medicine, Beijing, China.
Li-Feng
LF
Wang
Li
L
Wei
Si-Mo
SM
Qiao
Xiao-Na
XN
Gao
Ya-Bing
YB
Gao
Shui-Ming
SM
Wang
Li
L
Zhao
Ji
J
Dong
Xin-Ping
XP
Xu
Hong-Mei
HM
Zhou
Xiang-Jun
XJ
Hu
Rui-Yun
RY
Peng
D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics / Q000378:metabolism*; D000076122:Disks Large Homolog 4 Protein; D006624:Hippocampus / Q000737:chemistry / Q000166:cytology / Q000528:radiation effects* / Q000648:ultrastructure; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008872:Microwaves / Q000009:adverse effects*; D009474:Neurons / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D016716:PC12 Cells; D057907:Post-Synaptic Density / Q000528:radiation effects; D011499:Protein Processing, Post-Translational / Q000235:genetics / Q000528:radiation effects; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism* / Q000502:physiology; D015398:Signal Transduction; D009435:Synaptic Transmission / Q000528:radiation effects
D000076122:Disks Large Homolog 4 Protein; C501449:Dlg4 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D016194:Receptors, N-Methyl-D-Aspartate; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2