Live-streaming: Time-lapse video evidence of novel streamer formation mechanism and varying viscosity.
Time-lapse videos of growing biofilms were analyzed using a background subtraction method, which removed camouflaging effects from the heterogeneous field of view to reveal evidence of streamer formation from optically dense biofilm segments. In addition, quantitative measurements of biofilm velocity and optical density, combined with mathematical modeling, demonstrated that streamer formation occurred from mature, high-viscosity biofilms. We propose a streamer formation mechanism by sudden partial detachment, as opposed to continuous elongation as observed in other microfluidic studies. Additionally, streamer formation occurred in straight microchannels, as opposed to serpentine or pseudo-porous channels, as previously reported.
Département de Chimie, Université Laval , Québec, Canada.
Mazeyar
M
Parvinzadeh Gashti
Département de Chimie, Université Laval , Québec, Canada.
Julien
J
Bellavance
Department of Chemistry and Biology, Ryerson University , Toronto, Canada.
Otini
O
Kroukamp
Department of Microbiology, Stellenbosch University , Stellenbosch, South Africa.
Gideon
G
Wolfaardt
Département de Génie Chimique, Université Laval , Québec, Canada.
Seyed Mohammad
SM
Taghavi
Département de Chimie, Université Laval , Québec, Canada.
Jesse
J
Greener