MicroRNA-490-3p regulates cell proliferation and apoptosis by targeting HMGA2 in osteosarcoma.
MicroRNA-490-3p (miR-490-3p) has been implicated in several human malignancies; however, its potential functions and the underlying molecular mechanisms in osteosarcoma progression remain largely unclear. Here, we showed that miR-490-3p was down-regulated in osteosarcoma cell lines. Ectopic expression of miR-490-3p decreased cell proliferation, induced G1 arrest and apoptosis in vitro and inhibited tumorigenicity in a mouse xenograft model. Furthermore, miR-490-3p bound directly to HMGA2 mRNA 3'UTR and mediated a decrease in HMGA2 mRNA and protein expression. Re-expression of HMGA2 reversed the inhibitory effects of miR-490-3p. Further investigations showed an inverse correlation between low miR-490-3p and high HMGA2 expression in osteosarcoma tissues. Taken together, our results suggest that miR-490-3p functions as a potential tumor suppressor by down-regulating HMGA2 expression directly, and it may represent a potential therapeutic target for patients with osteosarcoma.
Department of Orthopaedics, Yidu Central Hospital, Weifang Medical University, Weifang, Shandong 262500, China. Electronic address: doone197901@sina.com.
Wei
W
Liu
Department of Orthopedics, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China.
Guoxing
G
Xu
Department of Orthopaedics, Yidu Central Hospital, Weifang Medical University, Weifang, Shandong 262500, China.
Huaqiang
H
Liu
Department of Orthopaedics, Yidu Central Hospital, Weifang Medical University, Weifang, Shandong 262500, China.
Tingting
T
Li
D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D025743:HMGA2 Protein / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009154:Mutation; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D014183:Transplantation, Heterologous
D020413:3' Untranslated Regions; D025743:HMGA2 Protein; C574862:MIRN490 microRNA, human; D035683:MicroRNAs
High mobility group a isoform 2; MicroRNA-490-3p; Osteosarcoma; Proliferation