Growth and Quantification of MERS-CoV Infection.
Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging highly pathogenic respiratory virus. Although MERS-CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS-CoV and RNA products of replication in vitro. MERS-CoV is able to infect a range of cell types, but replicates to high titers in Vero E6 cells. Protocols for the propagation and quantification of MERS-CoV are presented.
University of Maryland School of Medicine, Microbiology and Immunology, Baltimore, Maryland.
Christopher M
CM
Coleman
University of Maryland School of Medicine, Microbiology and Immunology, Baltimore, Maryland.
Matthew B
MB
Frieman
D000818:Animals; D002522:Chlorocebus aethiops; D065207:Middle East Respiratory Syndrome Coronavirus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D014709:Vero Cells; D019562:Viral Load / Q000379:methods*; D014776:Virus Cultivation / Q000379:methods*
D012367:RNA, Viral
MERS; coronavirus; infection; plaque assay; quantification; virology