Antrodia cinnamomea alleviates cisplatin-induced hepatotoxicity and enhances chemo-sensitivity of line-1 lung carcinoma xenografted in BALB/cByJ mice.
Whereas cisplatin (cis-diamminedichloroplatinum II) is a first-line medicine to treat solid cancerous tumors, it often causes serious side effects. New medicines that have an equivalent or even better therapeutic effect but with free or less side effects than cisplatin are highly anticipated in cancer therapy. Recent reports revealed that Antrodia cinnamomea (AC) possesses hepatoprotective activity in addition to anticancer. In this study, we wanted to know whether AC enhances chemo-sensitivity of cisplatin and/or alleviates cisplatin-induced hepatotoxicity, as well as the underlying mechanisms thereof. Our results indicated that AC inhibited proliferation of line-1 lung carcinoma cells and rescued hepatic HepG2 cells from cisplatin-induced cell death in vitro. The fact is that AC and cisplatin synergized to constrain growth of line-1 lung carcinoma cells in BALB/cByJ mice. Quantitative real-time PCR further revealed that AC promoted expression of apoptosis-related genes, while it decreased expression of NF-κB and VEGF in tumor tissues. In liver, AC reduced cisplatin-induced liver dysfunctions, liver inflammation and hepatic apoptosis in addition to body weight restoration. In summary, AC is able to increase cisplatin efficacy by triggering expression of apoptosis-related genes in line-1 lung cancer cells as well as to protect liver from tissue damage by avoiding cisplatin-induced hepatic inflammation and cell death.
Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Keelung, Taiwan.|Graduate Institute of Clinical Medicine Sciences, Chang Gung University, Taoyuan, Taiwan.
Tse-Hung
TH
Huang
Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan.
Yi-Han
YH
Chiu
Department of Life Science, Chinese Culture University, Taipei, Taiwan.
Yi-Lin
YL
Chan
Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan.|Institute of Biomedical Nutrition, Hung Kuang University, Taichung, Taiwan.
Hang
H
Wang
Genomics Research Center, Academia Sinica, Taipei, Taiwan.
Tsung-Lin
TL
Li
Department of Thoracic Medicine, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan, Taiwan.
Chien-Yin
CY
Liu
Department of Thoracic Medicine, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan, Taiwan.|Department of Respiratory Care, Chang Gung University, Taoyuan, Taiwan.
Cheng-Ta
CT
Yang
Graduate Institute of Clinical Medicine Sciences, Chang Gung University, Taoyuan, Taiwan.|Graduate Institute of Traditional Chinese Medicine, College of Medicine, Chang Gung University, Taoyuan, Taiwan.|School of Traditional Chinese Medicine, Chang Gung University, Taoyuan, Taiwan.
Tzung-Yan
TY
Lee
Graduate Institute of Clinical Medicine Sciences, Chang Gung University, Taoyuan, Taiwan.|Graduate Institute of Traditional Chinese Medicine, College of Medicine, Chang Gung University, Taoyuan, Taiwan.|School of Traditional Chinese Medicine, Chang Gung University, Taoyuan, Taiwan.
Jyh-Sheng
JS
You
Graduate Institute of Clinical Medicine Sciences, Chang Gung University, Taoyuan, Taiwan.|Laboratory for Epidemiology, Department and Graduate Institute of Health Care Management, Chang Gung University, Taoyuan, Taiwan.
Kuang-Hung
KH
Hsu
Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan.|Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan.
Chang-Jer
CJ
Wu
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D055491:Antrodia / Q000737:chemistry*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D002945:Cisplatin / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
D000970:Antineoplastic Agents; D000975:Antioxidants; D051017:Apoptosis Regulatory Proteins; D016328:NF-kappa B; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D002945:Cisplatin
Antrodia cinnamomea; cisplatin; hepatotoxicity; lung cancer