Effects of culture conditions on monosaccharide composition of Ganoderma lucidum exopolysaccharide and on activities of related enzymes.
We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values. The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities. In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
Key Laboratory of Carbohydrate Chemistry & Biotechnology Ministry of Education, & School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Lin
L
Peng
Key Laboratory of Carbohydrate Chemistry & Biotechnology Ministry of Education, & School of Biotechnology, Jiangnan University, Wuxi 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China.
Shuangkui
S
Qiao
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
Zhenghong
Z
Xu
Key Laboratory of Carbohydrate Chemistry & Biotechnology Ministry of Education, & School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Feng
F
Guan
Key Laboratory of Carbohydrate Chemistry & Biotechnology Ministry of Education, & School of Biotechnology, Jiangnan University, Wuxi 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China. Electronic address: bioding@163.com.
Zhongyang
Z
Ding
National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China.
Zhenghua
Z
Gu
National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China.
Liang
L
Zhang
National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China.
Guiyang
G
Shi
D045744:Cell Line, Tumor; D046508:Culture Techniques / Q000379:methods*; D005285:Fermentation; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D032902:Ganoderma / Q000737:chemistry* / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D009005:Monosaccharides / Q000032:analysis*
D062610:Fungal Polysaccharides; D009005:Monosaccharides; D005947:Glucose
Exopolysaccharide; Ganoderma lucidum; Monosaccharide composition; PGI; PGM