The nutrigenetic influence of the interaction between dietary vitamin E and TXN and COMT gene polymorphisms on waist circumference: a case control study.
BACKGROUND
Abdominal obesity (AO) is a common modifiable risk factor for certain non-communicable diseases associated with enhanced oxidative stress (OS). The objective of this work was to investigate whether the interaction between antioxidant vitamin intake and OS-related polymorphisms modulates gene-associated anthropometry in a Spanish population.


METHODS
A total of 246 subjects with AO, and 492 age and gender matched non-AO subjects were included in the study. Anthropometric, biochemical, and OS parameters, and antioxidant dietary intake data were assessed using validated procedures. DNA from white blood cells was isolated and the genotype of seven polymorphisms from genes involved in OS (pro-oxidant and antioxidant) were analyzed using the SNPlex system. The effects of the c.-793T > C polymorphism on promoter activity and thus thioredoxin (TXN) activity were examined using reporter assays.


RESULTS
The AO group had higher 8-Oxo-2'-deoxyguanosine levels and took in less vitamin A and vitamin E compared to the non-AO group. Logistic regression analysis revealed that the rs2301241 polymorphism in TXN and rs740603 in catechol-O-methyltransferase (COMT) were associated with waist circumference (WC) and AO. Moreover, these polymorphisms were more strongly associated with variations in WC in subjects with low vitamin E intakes. A promoter assay revealed that the T to C conversion at c.-793 (rs2301241) induced a more than two fold increase in reporter gene expression.


CONCLUSIONS
WC is associated both with dietary vitamin E intake and genetic variants of TXN and COMT suggesting that existence of a complex nutrigenetic pathway that involves regulation of AO.
CIBER Physiopathology of Obesity and Nutrition CIBERobn, CB06/03, Institute of Health Carlos III, c/Sinesio Delgado 6, 28029, Madrid, Spain. m.luisa.mansego@uv.es.|Genotyping and Genetic Diagnosis Unit, Fundación de Investigación del Hospital Clínico de Valencia-INCLIVA, Av. Blasco Ibañez 17, 46010, Valencia, Spain. m.luisa.mansego@uv.es.
Maria L
ML
Mansego
Genotyping and Genetic Diagnosis Unit, Fundación de Investigación del Hospital Clínico de Valencia-INCLIVA, Av. Blasco Ibañez 17, 46010, Valencia, Spain. griselda.marco@uv.es.|CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), c/Sinesio Delgado 6, 28029, Madrid, Spain. griselda.marco@uv.es.
Griselda
G
De Marco
CIBER Physiopathology of Obesity and Nutrition CIBERobn, CB06/03, Institute of Health Carlos III, c/Sinesio Delgado 6, 28029, Madrid, Spain. Carmen.ivorra@uv.es.|Cardiovascular Risk Unit, Consorcio, Hospital General, University of Valencia, Av. Tres Cruces 2, 46014, Valencia, Spain. Carmen.ivorra@uv.es.
Carmen
C
Ivorra
Internal Medicine Unit, Rio Hortega Hospital, c/Dulzaina 2, 47012, Valladolid, Spain. rulo636@yahoo.es.
Raúl
R
Lopez-Izquierdo
CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), c/Sinesio Delgado 6, 28029, Madrid, Spain. sonsoles75@gmail.com.|Endocrinology and Nutrition Department, Carlos Haya University Hospital and Instituto de Investigación Biomédica de Málaga (IBIMA), c/Jorge Luis Borges 15, 29010, Málaga, Spain. sonsoles75@gmail.com.
Sonsoles
S
Morcillo
CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), c/Sinesio Delgado 6, 28029, Madrid, Spain. gemma.rojo.m@gmail.com.|Endocrinology and Nutrition Department, Carlos Haya University Hospital and Instituto de Investigación Biomédica de Málaga (IBIMA), c/Jorge Luis Borges 15, 29010, Málaga, Spain. gemma.rojo.m@gmail.com.
Gemma
G
Rojo-Martínez
Genotyping and Genetic Diagnosis Unit, Fundación de Investigación del Hospital Clínico de Valencia-INCLIVA, Av. Blasco Ibañez 17, 46010, Valencia, Spain. Veronica.Gonzalez@uv.es.
Verónica
V
González-Albert
CIBER Physiopathology of Obesity and Nutrition CIBERobn, CB06/03, Institute of Health Carlos III, c/Sinesio Delgado 6, 28029, Madrid, Spain. fernandoctor@hotmail.com.|Hypertension Clinic, Hospital Clínico Universitario de Valencia, Av. Blasco Ibañez 15, 46010, Valencia, Spain. fernandoctor@hotmail.com.
Fernando
F
Martinez
CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), c/Sinesio Delgado 6, 28029, Madrid, Spain. federico.soriguer.sspa@juntadeandalucia.es.|Endocrinology and Nutrition Department, Carlos Haya University Hospital and Instituto de Investigación Biomédica de Málaga (IBIMA), c/Jorge Luis Borges 15, 29010, Málaga, Spain. federico.soriguer.sspa@juntadeandalucia.es.
Federico
F
Soriguer
Internal Medicine Unit, Rio Hortega Hospital, c/Dulzaina 2, 47012, Valladolid, Spain. juancarlos.martinescudero@gmail.com.
Juan C
JC
Martín-Escudero
CIBER Physiopathology of Obesity and Nutrition CIBERobn, CB06/03, Institute of Health Carlos III, c/Sinesio Delgado 6, 28029, Madrid, Spain. josep.redon@uv.es.|Hypertension Clinic, Hospital Clínico Universitario de Valencia, Av. Blasco Ibañez 15, 46010, Valencia, Spain. josep.redon@uv.es.
Josep
J
Redon
Genotyping and Genetic Diagnosis Unit, Fundación de Investigación del Hospital Clínico de Valencia-INCLIVA, Av. Blasco Ibañez 17, 46010, Valencia, Spain. felipe.chaves@uv.es.|CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), c/Sinesio Delgado 6, 28029, Madrid, Spain. felipe.chaves@uv.es.
F Javier
FJ
Chaves
D000328:Adult; D000367:Age Factors; D000368:Aged; D000886:Anthropometry; D000975:Antioxidants / Q000737:chemistry; D016022:Case-Control Studies; D002394:Catechol O-Methyltransferase / Q000235:genetics*; D004032:Diet; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D054647:Nutrigenomics; D009765:Obesity / Q000235:genetics*; D018384:Oxidative Stress; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012737:Sex Factors; D013030:Spain; D013879:Thioredoxins / Q000737:chemistry / Q000235:genetics*; D014810:Vitamin E / Q000737:chemistry*; D055105:Waist Circumference
D000975:Antioxidants; C491203:TXN protein, human; D014810:Vitamin E; D013879:Thioredoxins; C588010:COMT protein, human; D002394:Catechol O-Methyltransferase