A novel diagnostic approach to detecting porcine epidemic diarrhea virus: The lateral immunochromatography assay.
Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration in sucking piglets and has a high mortality rate. An immunochromatography (IC) assay, known as a lateral flow test, is a simple device intended to detect the presence of target pathogens. Here, we developed an IC assay that detected PEDV antigens with 96.0% (218/227) sensitivity and 98.5% (262/266) specificity when compared with real-time reverse transcriptase (RT)-PCR using FAM-labeled probes based on sequences from nucleocapsid genes. The detection limits of the real-time RT-PCR and IC assays were 1×10(2) and 1×10(3) copies, respectively. The IC assay developed herein did not detect non-specific reactions with other viral or bacterial pathogens, and the assay could be stored at 4°C or room temperature for 15 months without affecting its efficacy. Thus, the IC assay may result in improved PED detection and control on farms, and is a viable alternative to current diagnostic tools for PEDV.
Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, Republic of Korea.
Yong Kwan
YK
Kim
Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, Republic of Korea.
Seong-In
SI
Lim
Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, Republic of Korea.
In-Soo
IS
Cho
Median Diagnostics Inc., Chuncheon, Gangwon-do 200-883, Republic of Korea.
Kwang-Myun
KM
Cheong
Median Diagnostics Inc., Chuncheon, Gangwon-do 200-883, Republic of Korea.
Eun-Jeong
EJ
Lee
Median Diagnostics Inc., Chuncheon, Gangwon-do 200-883, Republic of Korea.
Sang-Oh
SO
Lee
Median Diagnostics Inc., Chuncheon, Gangwon-do 200-883, Republic of Korea.
Joon-Bae
JB
Kim
Median Diagnostics Inc., Chuncheon, Gangwon-do 200-883, Republic of Korea.
Jung-Hwa
JH
Kim
Gangwon-do Veterinary Service Laboratory, Chuncheon, Gangwon-do 200-822, Republic of Korea.
Dong-Soo
DS
Jeong
Applied Chemistry and Biological Engineering, Ajou University, Suwon 443-749, Republic of Korea.
Byung-Hyun
BH
An
Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, Republic of Korea. Electronic address: andj67@korea.kr.
Dong-Jun
DJ
An
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods*; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D053485:Porcine epidemic diarrhea virus / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology*
D000956:Antigens, Viral
Immunochromatography; Lateral flow test; Porcine epidemic diarrhea virus; Sensitivity; Specificity