A Simple and Sensitive Method to Quantify Biodegradable Nanoparticle Biodistribution using Europium Chelates.
The biodistribution of biodegradable nanoparticles can be difficult to quantify. We report a method using time resolved fluorescence (TRF) from a lanthanide chelate to minimize background autofluorescence and maximize the signal to noise ratio to detect biodegradable nanoparticle distribution in mice. Specifically, antenna chelates containing europium were entrapped within nanoparticles composed of polylactic acid-polyethylene glycol diblock copolymers. Tissue accumulation of nanoparticles following intravenous injection was quantified in mice. The TRF of the nanoparticles was found to diminish as a second order function in the presence of serum and tissue compositions interfered with the europium signal. Both phenomena were corrected by linearization of the signal function and calculation of tissue-specific interference, respectively. Overall, the method is simple and robust with a detection limit five times greater than standard fluorescent probes.
School of Chemical and Biomolecular Engineering, Cornell University, Ithaca NY.
Lindsey
L
Crawford
Department of Biological and Environmental Engineering, Cornell University, Ithaca NY.
Jaclyn
J
Higgins
School of Chemical and Biomolecular Engineering, Cornell University, Ithaca NY.|Department of Biomedical Engineering, Cornell University, Ithaca NY.
David
D
Putnam
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002614:Chelating Agents / Q000378:metabolism*; D005063:Europium / Q000378:metabolism*; D007773:Lactates; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry; D011092:Polyethylene Glycols; D013050:Spectrometry, Fluorescence / Q000379:methods; D014018:Tissue Distribution
D001672:Biocompatible Materials; D002614:Chelating Agents; D007773:Lactates; C432470:polylactic acid-dioxanone-polyethylene glycol block copolymer; D011092:Polyethylene Glycols; D005063:Europium