Immobilization of Antibody on a Cyclic Olefin Copolymer Surface with Functionalizable, Non-Biofouling Poly[Oligo(Ethylene Glycol) Methacrylate].
We report a perfluoroaryl azide-based photoreaction for synthesizing functionalizable and nonbiofouling poly[oligo(ethylene glycol) methacrylate] (pOEGMA) films on a chemically inert COC substrate, and an estimation of a surface coverage of the antibody immobilized onto the surface with the immuno-gold nanoparticles. The processes were confirmed by water contact angle measurement, FT-IR spectroscopy, and FE-SEM. The strategy demonstrated in this work could be applied to functionalizations of other polymeric materials and determination of the binding capacity of analytes in biosensors and microfluidic devices.
Seung Pyo
SP
Jeong
Sung Min
SM
Kang
Daewha
D
Hong
Hee-Yoon
HY
Lee
Insung S
IS
Choi
Sangwon
S
Ko
Jungkyu K
JK
Lee
D000327:Adsorption; D000475:Alkenes / Q000737:chemistry*; D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D006046:Gold / Q000737:chemistry*; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D058726:Nanoconjugates / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties
D000475:Alkenes; D000906:Antibodies; D007074:Immunoglobulin G; D008689:Methacrylates; D058726:Nanoconjugates; C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; D006046:Gold