Aptamer-based surface-enhanced Raman scattering-microfluidic sensor for sensitive and selective polychlorinated biphenyls detection.
A surface-enhanced Raman scattering (SERS) measurement of 3,3',4,4'-tetrachlorobiphenyl (PCB77) with aptamer capturing in a microfluidic device was demonstrated. To construct the microfluidic chip, an ordered Ag nanocrown array was fabricated over a patterned polydimethylsiloxane (PDMS) that was achieved by replicating an anodic aluminum oxide (AAO) template. The patterned PDMS sheet was covered with another PDMS sheet having two input channel grooves to form a close chip. The Ag nanocrown array was used for the SERS enhancement area and the detection zone. PCB 77 aptamers were injected into one channel and the other allows for analytes (PCBs). The mercapto aptamers captured the targets in the mixed zone and were immobilized to the SERS detection zone via S-Ag bonds so as to further improve both the SERS sensitivity and selectivity of PCB77. Such an aptamer-based microfluidic chip realized a rapid SERS detection. The lowest detectable concentration of 1.0 × 10(-8) M was achieved for PCB77. This work demonstrates that the aptamer-modified SERS microfluidic sensor can be utilized for selective detections of organic pollutants in the environment.
State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University , 2699 Qianjin Avenue, Changchun 130012, P. R. China.
Cuicui
C
Fu
State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University , 2699 Qianjin Avenue, Changchun 130012, P. R. China.
Yi
Y
Wang
State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University , 2699 Qianjin Avenue, Changchun 130012, P. R. China.|State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University , 2699 Qianjin Avenue, Changchun, The People's Republic of China.
Gang
G
Chen
State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University , 2699 Qianjin Avenue, Changchun 130012, P. R. China.
Liyuan
L
Yang
State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University , 2699 Qianjin Avenue, Changchun 130012, P. R. China.
Shuping
S
Xu
State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University , 2699 Qianjin Avenue, Changchun 130012, P. R. China.
Weiqing
W
Xu
D000537:Aluminum Oxide / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000295:instrumentation; D013499:Surface Properties
D052157:Aptamers, Nucleotide; D012834:Silver; D011078:Polychlorinated Biphenyls; D000537:Aluminum Oxide; C028451:3,4,3',4'-tetrachlorobiphenyl