Gensenoside Rb1 protects rat PC12 cells from oxidative stress-induced endoplasmic reticulum stress: the involvement of thioredoxin-1.
Oxidative stress plays an important role in many diseases and hydrogen peroxide (H2O2) plays a central role in the stress. Gensenoside Rb1 is the one of active ingredients in the traditional Chinese medicine Panax notoginseng. It has been reported that gensenoside Rb1 possesses various pharmacological activities. Here we report that gensenoside Rb1 exhibits potent protective effects against oxidative injury induced by H2O2 through inhibiting endoplasmic reticulum stress in PC12 cells. Cell viability assay demonstrated that incubation with H2O2 for 24 h led to a significant loss of cultured rat PC12 cells, and the cell viability was pronouncedly increased by pretreatment of gensenoside Rb1 for 24 h. H2O2-induced endoplasmic reticulum stress pathway was also suppressed after gensenoside Rb1 pretreatment, which was related with thioredoxin-1 (Trx-1) induction. Trx-1 siRNA abolished the protective effects of gensenoside Rb1. Our results of the present study demonstrate that gensenoside Rb1 shows a potent anti-oxidative effect on cultured PC12 cells by inducing Trx-1 expression.
College of Life Science, Xinyang Normal University, Xinyang, 464000, China.
Xian-Si
XS
Zeng
College of Life Science, Xinyang Normal University, Xinyang, 464000, China. jiajinjing1986@126.com.
Jin-Jing
JJ
Jia
College of Life Science, Xinyang Normal University, Xinyang, 464000, China.
Li-Fang
LF
Ma
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D036145:Ginsenosides / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000633:toxicity; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D034622:RNA Interference; D051381:Rats; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D000975:Antioxidants; D036145:Ginsenosides; D018696:Neuroprotective Agents; D016877:Oxidants; C495697:Txn1 protein, rat; D013879:Thioredoxins; C442759:ginsenoside Rb1; D006861:Hydrogen Peroxide
Endoplasmic reticulum stress; Gensenoside Rb1; Oxidative stress; Thioredoxin-1