Coupling a universal DNA circuit with graphene sheets/polyaniline/AuNPs nanocomposites for the detection of BCR/ABL fusion gene.
This article described a novel method by coupling a universal DNA circuit with graphene sheets/polyaniline/AuNPs nanocomposites (GS/PANI/AuNPs) for highly sensitive and specific detection of BCR/ABL fusion gene (bcr/abl) in chronic myeloid leukemia (CML). DNA circuit known as catalyzed hairpin assembly (CHA) is enzyme-free and can be simply operated to achieve exponential amplification, which has been widely employed in biosensing. However, application of CHA has been hindered by the need of specially redesigned sequences for each single-stranded DNA input. Herein, a transducer hairpin (HP) was designed to obtain a universal DNA circuit with favorable signal-to-background ratio. To further improve signal amplification, GS/PANI/AuNPs with excellent conductivity and enlarged effective area were introduced into this DNA circuit. Consequently, by combining the advantages of CHA and GS/PANI/AuNPs, bcr/abl could be detected in a linear range from 10 pM to 20 nM with a detection limit of 1.05 pM. Moreover, this protocol showed excellent specificity, good stability and was successfully applied for the detection of real sample, which demonstrated its great potential in clinical application.
Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China.
Xueping
X
Chen
Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China; Department of Medical Laboratory, Chongqing Emergency Medical Center (Chongqing The Fourth Hospital), Chongqing, 400016, PR China.
Li
L
Wang
The No.2 Peoples' Hospital of Yibin, Sichuan, 644000, PR China.
Shangchun
S
Sheng
Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China.
Teng
T
Wang
Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China.
Juan
J
Yang
Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: guomingxie@cqmu.edu.cn.
Guoming
G
Xie
Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: fengwlcqmu@sina.com.
Wenli
W
Feng
D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques; D004273:DNA, Neoplasm / Q000032:analysis*; D055664:Electrochemical Techniques; D016044:Fusion Proteins, bcr-abl / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D016133:Polymerase Chain Reaction
D000814:Aniline Compounds; D004273:DNA, Neoplasm; C416807:polyaniline; D006046:Gold; D006108:Graphite; D016044:Fusion Proteins, bcr-abl
BCR/ABL fusion gene; Catalyzed hairpin assembly; Electrochemical DNA biosensor; Transducer hairpin; Universal DNA circuit