Lack of Association between Polymorphisms of the TLR4 Gene and Infection with the Hepatitis B and C Viruses.
Toll-like receptor 4 (TLR4) plays a crucial role in the early recognition of pathogenic microorganisms and provides an ideal model to investigate the consequences of genetic variation and susceptibility to diseases. The present study investigated the occurrence of the single nucleotide polymorphisms (SNPs) rs4986790 (A>G) and rs4986791 (C>T) in the TLR4 gene in chronic carriers of the hepatitis B (HBV) and C (HCV) viruses. A total of 420 blood samples were collected (HBV, 49; HCV, 72; and controls, 299) at the liver disease outpatient clinic of Hospital da Fundação Santa Casa de Misericórdia do Pará (FSCMPA). Genomic DNA extracted from leukocytes was subjected to real-time polymerase chain reaction (qPCR) analysis to identify the genetic profile of the participants. No significant differences were found in the allele and genotype frequencies between the infected participants and controls. No significant associations were found between the investigated polymorphisms and inflammatory activity, fibrosis, and the presence of cirrhosis; the same results were obtained in the haplotype analysis. The results showed a lack of association between the rs4986790 and rs4986791 SNPs and susceptibility to infection with HBV and HCV, as well as clinical and laboratory information of the patients.
Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
Orlando de Souza
Ode S
Pires-Neto
Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
Keyla Santos Guedes
KS
de Sá
Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
Barbara Brasil
BB
Santana
Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
Samara Tatielle Monteiro
ST
Gomes
Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
Ednelza da Silva Graça
Eda S
Amoras
Faculdade de Medicina, Instituto de Ciências da Saúde, Universidade Federal do Pará, Umarizal, 66050-060 Belém, PA, Brazil.
Simone Regina da Silva
SR
Conde
Faculdade de Medicina, Instituto de Ciências da Saúde, Universidade Federal do Pará, Umarizal, 66050-060 Belém, PA, Brazil.
Sâmia
S
Demachki
Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
Vânia Nakauth
VN
Azevedo
Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
Rosimar Neris
RN
Martins-Feitosa
Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
Marluísa de Oliveira Guimarães
Mde O
Ishak
Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
Ricardo
R
Ishak
Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
Antonio Carlos Rosário
AC
Vallinoto
D000328:Adult; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006509:Hepatitis B / Q000235:genetics*; D006526:Hepatitis C / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics*
C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4