Estimation of multiexponential fluorescence decay parameters using compressive sensing.
Fluorescence lifetime imaging microscopy (FLIM) is a microscopic imaging technique to present an image of fluorophore lifetimes. It circumvents the problems of typical imaging methods such as intensity attenuation from depth since a lifetime is independent of the excitation intensity or fluorophore concentration. The lifetime is estimated from the time sequence of photon counts observed with signal-dependent noise, which has a Poisson distribution. Conventional methods usually estimate single or biexponential decay parameters. However, a lifetime component has a distribution or width, because the lifetime depends on macromolecular conformation or inhomogeneity. We present a novel algorithm based on a sparse representation which can estimate the distribution of lifetime. We verify the enhanced performance through simulations and experiments.
Ewha Womans University Medical Center, Institute of Convergence Medicine, 1071, Anyangcheon-ro, Yangcheon-gu, Seoul 158-710, Republic of Korea.
Sejung
S
Yang
Ewha Womans University, Department of Electronics Engineering, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of Korea.
Joohyun
J
Lee
Ewha Womans University, Department of Chemistry and Nanoscience, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of Korea.
Youmin
Y
Lee
Ewha Womans University, Department of Chemistry and Nanoscience, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of Korea.
Minyung
M
Lee
Ewha Womans University, Department of Electronics Engineering, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of Korea.
Byung-Uk
BU
Lee
D000465:Algorithms; D003198:Computer Simulation; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D016012:Poisson Distribution; D012815:Signal Processing, Computer-Assisted
D005456:Fluorescent Dyes