Characterization of the In Situ Ecophysiology of Novel Phylotypes in Nutrient Removal Activated Sludge Treatment Plants.
An in depth understanding of the ecology of activated sludge nutrient removal wastewater treatment systems requires detailed knowledge of the community composition and metabolic activities of individual members. Recent 16S rRNA gene amplicon surveys of activated sludge wastewater treatment plants with nutrient removal indicate the presence of a core set of bacterial genera. These organisms are likely responsible for the bulk of nutrient transformations underpinning the functions of these plants. While the basic activities of some of these genera in situ are known, there is little to no information for the majority. This study applied microautoradiography coupled with fluorescence in situ hybridization (MAR-FISH) for the in situ characterization of selected genus-level-phylotypes for which limited physiological information is available. These included Sulfuritalea and A21b, both within the class Betaproteobacteria, as well as Kaga01, within sub-group 10 of the phylum Acidobacteria. While the Sulfuritalea spp. were observed to be metabolically versatile, the A21b and Kaga01 phylotypes appeared to be highly specialized.
Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.
Simon Jon
SJ
McIlroy
Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark; EcoTopia Science Institute, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8603, Japan; Department of Civil and Environmental Engineering, Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashihiroshima, 739-8527, Japan.
Takanori
T
Awata
Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.
Marta
M
Nierychlo
Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.
Mads
M
Albertsen
Department of Civil and Environmental Engineering, Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashihiroshima, 739-8527, Japan.
Tomonori
T
Kindaichi
Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.
Per Halkjær
PH
Nielsen
D061271:Acidobacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001345:Autoradiography; D020562:Betaproteobacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes / Q000737:chemistry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D059013:Microbial Consortia / Q000502:physiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid
D015342:DNA Probes; D012336:RNA, Ribosomal, 16S; D012722:Sewage