Design, synthesis, and delivery studies of organotin(IV) based HCV inhibitor.
Tributylstannic[3-(3,5 -dimethylphenylamido)propionate] is synthesized and characterized by elemental analysis, FT-IR, multinuclear NMR ((1)H, (13)C and (119)Sn) and mass spectrometry. The organic anion was found to act as monodentate O-bound ligand in solution. The compound was screened for the anti-HCV potency by the Gaussia luciferase Assay using infected Huh 7.5 cells (human hepatocellular cell) and is found active against HCV with logIC50 1.2nM in the cell-based assay. Cationic surfactant cetyl N,N,N-trimethylammoniumbromide (CTAB) was used to study the interactions of the organotin(IV) complex with positively charged micelles of the surfactant acting as a model cell membrane. The thermodynamics parameters of complex- CTAB interaction concluded that the complex is located in the palisade layer of CTAB micelles. The increase in absorbance of visible spectra of the compound confirmed its solubilization into micelles. The two carbonyl oxygen's were found to be binding sites of the complex with CTAB.
Farooq A
FA
Shah
Kaneez
K
Fatima
Saqib
S
Ali
King Fahd Medical Research Center, King Abdul Aziz University, Saudi Arabia. Ishtiaq80262@yahoo.com.
Ishtiaq
I
Qadri
D000998:Antiviral Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry / Q000494:pharmacology; D055808:Drug Discovery; D016174:Hepacivirus / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008024:Ligands; D008823:Micelles; D017550:Spectroscopy, Fourier Transform Infrared; D014220:Trialkyltin Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D002593:Cetrimonium Compounds; D008024:Ligands; D008823:Micelles; D014220:Trialkyltin Compounds; C000609907:tributylstannic (3-(3,5-dimethylphenylamido)propionate); D000077286:Cetrimonium