Paraglycomyces xinjiangensis gen. nov., sp. nov., a halophilic actinomycete.
A novel halophilic actinobacterium, designated strain TRM 49201T, was isolated from a hypersaline soil in Xinjiang Province, north-west China. The strain was aerobic, Gram-stain-positive and halophilic. The aerial mycelium was chaotic with irregular branches, and spherical sporangia containing several spherical spores developed at mycelial aggregations. The strain had an optimum NaCl concentration for growth of 8-13 % (w/v). The whole-cell sugar pattern of strain TRM 49201T consisted of xylose and ribose. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-10(H2). The polar lipids were diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phosphatidylglycerol, phosphatidylcholine and four unknown phospholipids. The major fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 70 mol%. Phylogenetic analysis showed that strain TRM 49201T can be distinguished from representatives of Glycomyces, Stackebrandtia and Haloglycomyces, the three existing genera in the family Glycomycetaceae, based on low 16S rRNA gene sequence similarities ( < 94.42 %). Strain TRM 49201T is thus considered to represent a novel species of a new genus in the family Glycomycetaceae, for which the name Paraglycomyces xinjiangensis gen. nov., sp. nov. is proposed. The type strain of Paraglycomyces xinjiangensis is TRM 49201T ( = NRRL B-24926T = CCTCC AA 2013002T = KACC 17683T).
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps/College of Life Science, Tarim University, Alar 843300, PR China.
Xiao-Xia
XX
Luo
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps/College of Life Science, Tarim University, Alar 843300, PR China.
Xiao-Xue
XX
Han
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps/College of Life Science, Tarim University, Alar 843300, PR China.
Fei
F
Zhang
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps/College of Life Science, Tarim University, Alar 843300, PR China.
Chuan-Xing
CX
Wan
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps/College of Life Science, Tarim University, Alar 843300, PR China.
Li-Li
LL
Zhang
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D014451:Ubiquinone; C073355:menaquinone 9; C025204:Ubiquinone Q2