Expression of Phospholipase D in Periodontitis and Its Role in the Inflammatory and Osteoclastic Response by Nicotine- and Lipopolysaccharide-Stimulated Human Periodontal Ligament Cells.
BACKGROUND
The aim of the present study is to investigate the expression of phospholipase D (PLD) 1 and PLD2 in periodontal patients and in human periodontal ligament cells (HPDLCs) exposed to nicotine plus lipopolysaccharide (LPS) from Porphyromonas gingivalis (Toll-like receptor 2 ligand). Furthermore, the effects of PLD isoform inhibition on the inflammatory response and osteoclast differentiation and its mechanisms were determined.


METHODS
Proinflammatory mediators were examined by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. To silence the gene expression of the PLD isoforms, cells were transfected with small interfering RNA (siRNA) targeting PLD1 or PLD2. Mouse bone marrow-derived macrophages (BMMs) were used as osteoclast precursor cells for in vitro osteoclastogenesis. Western blot analysis and immunofluorescence were used to assess signaling pathways.


RESULTS
Chronic smokers with periodontitis exhibited significantly higher PLD1 and PLD2 messenger RNA (mRNA) expression than non-smokers with periodontitis and healthy controls. Nicotine and LPS upregulated PLD1 and PLD2 mRNA expression in a dose-dependent manner in HPDLCs. Pharmacologic and siRNA-mediated inhibition of PLD1 and PLD2 attenuated the nicotine- and LPS-induced upregulation of inducible nitric oxide (NO) synthase and cyclooxygenase-2, production of NO, and prostaglandin E2, and mRNA expression and secretion of tumor necrosis factor-α, interleukin (IL)-1β, and IL-8. The conditioned media from HPDLCs treated with PLD isoform inhibitors or siRNA against PLD inhibited receptor activator of nuclear factor-κB (NF-κB) ligand-mediated osteoclast differentiation, as well as protein expression of nuclear factor of activated T cells c1 and c-Fos, in BMMs. In addition, PLD isoform inhibitors and siRNA inhibited the nicotine- and LPS-induced activation of phosphoinositide 3-kinase, protein kinase C, p38, extracellular signal-regulated kinase, c-Jun N-terminal protein kinase, mitogen-activated protein kinase, and NF-κB.


CONCLUSIONS
To the best of the authors' knowledge, this study is the first to demonstrate that PLD isoform inhibition has anti-inflammatory and antiosteoclastogenic effects and thus may be a therapeutic target for the treatment of periodontitis.
Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
Seung-Yun
SY
Shin
Department of Oral and Maxillofacial Pathology and Research Center for Tooth and Periodontal Regeneration, School of Dentistry, Kyung Hee University.
Young-Suk
YS
Kim
Department of Oral and Maxillofacial Pathology and Research Center for Tooth and Periodontal Regeneration, School of Dentistry, Kyung Hee University.
So-Youn
SY
Lee
Department of Oral and Maxillofacial Pathology and Research Center for Tooth and Periodontal Regeneration, School of Dentistry, Kyung Hee University.
Won-Jung
WJ
Bae
Department of Preventive and Society Dentistry, School of Dentistry, Kyung Hee University.
Yong-Duk
YD
Park
Department of Preventive and Society Dentistry, School of Dentistry, Kyung Hee University.
Yong-Cheol
YC
Hyun
Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
KyungLhi
K
Kang
Department of Oral and Maxillofacial Pathology and Research Center for Tooth and Periodontal Regeneration, School of Dentistry, Kyung Hee University.
Eun-Cheol
EC
Kim
D000818:Animals; D006801:Humans; D008070:Lipopolysaccharides; D009538:Nicotine; D010010:Osteoclasts; D010513:Periodontal Ligament; D010518:Periodontitis; D019869:Phosphatidylinositol 3-Kinases; D010739:Phospholipase D
D008070:Lipopolysaccharides; D009538:Nicotine; D019869:Phosphatidylinositol 3-Kinases; D010739:Phospholipase D
Anti-inflammatory agents; osteoclasts; periodontal diseases; phospholipase D