Modulation of Amyloid-β Fibrils into Mature Microrod-Shaped Structure by Histidine Functionalized Water-Soluble Perylene Diimide.
Alzheimer's disease (AD) is associated with different types of amyloid peptide aggregates including senile plaques, fibrils, protofibrils, and oligomers. Due to these difficulties, a powerful strategy is needed for the disaggregation of amyloid aggregates by modulating their self-aggregation behavior. Herein, we report a unique approach toward transforming the aggregated amyloidogenic peptides using an amino acid functionalized perylene diimide as a molecular modulator, which is a different nondestructive approach as compared to inhibiting the aggregation of peptides. The histidine functionalized perylenediimide (PDI-HIS) molecule could coassemble with amyloid β (Aβ) peptides via hydrogen bonding that leads to the enhancement in the π-π interactions between Aβ and PDI-HIS moieties. The Thioflavin T (ThT) assay and various spectroscopic and microscopic techniques establish that the PDI-HIS molecules accelerate the Aβ1-40 and the amyloid aggregates in CSF into micro size coassembled structures. These results give rise to a new and unique complementary approach for modulating the biological effects of the aggregates in amyloidogenic peptides.
Department of Chemistry, Indian Institute of Technology , Guwahati 781039, Assam, India.
Balakrishnan
B
Muthuraj
Department of Chemistry, Indian Institute of Technology , Guwahati 781039, Assam, India.
Sayan Roy
SR
Chowdhury
Department of Chemistry, Indian Institute of Technology , Guwahati 781039, Assam, India.|Center for Nanotechnology, Indian Institute of Technology , Guwahati 781039, Assam, India.
Parameswar K
PK
Iyer
D016229:Amyloid beta-Peptides / Q000737:chemistry*; D052160:Benzothiazoles; D000067493:Dynamic Light Scattering; D031541:Fluorescence Resonance Energy Transfer; D006639:Histidine / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D007094:Imides / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D066329:Protein Aggregates; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013844:Thiazoles / Q000378:metabolism; D014867:Water / Q000737:chemistry*
D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D007094:Imides; D066329:Protein Aggregates; D013844:Thiazoles; C521332:perylenediimide; D014867:Water; C009462:thioflavin T; D006639:Histidine; D010569:Perylene
aggregation; amyloid-β peptides; molecular modulator; neurodegenerative disease; perylene diimide; self-assembly