Ficolin B in Diabetic Kidney Disease in a Mouse Model of Type 1 Diabetes.
BACKGROUND
The innate immune system may have adverse effects in diabetes and cardiovascular disease. The complement system seems to play a key role through erroneous complement activation via hyperglycaemia-induced neoepitopes. Recently mannan-binding lectin (MBL) was shown to worsen diabetic kidney changes. We hypothesize that mouse ficolin B exerts detrimental effects in the diabetic kidney as seen for MBL.


METHODS
We induced diabetes with streptozotocin in female wild-type mice and ficolin B knockout mice and included two similar nondiabetic groups. Renal hypertrophy and excretion of urinary albumin and creatinine were quantified to assess diabetic kidney damage.


RESULTS
In the wild-type groups, the kidney weighed 24% more in the diabetic mice compared to the controls. The diabetes-induced increase in kidney weight was 29% in the ficolin B knockout mice, that is, equal to wild-type animals (two-way ANOVA, P = 0.60). In the wild-type mice the albumin-to-creatinine ratio (ACR) was 32.5 mg/g higher in the diabetic mice compared to the controls. The difference was 62.5 mg/g in the ficolin B knockout mice, but this was not significantly different from the wild-type animals (two-way ANOVA, P = 0.21).


CONCLUSIONS
In conclusion, the diabetes-induced effects on kidney weight and ACR were not modified by the presence or absence of ficolin B.
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, 8000 Aarhus, Denmark ; Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark.
Charlotte Berg
CB
Holt
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, 8000 Aarhus, Denmark ; The Danish Diabetes Academy, Sdr. Boulevard 29, 5000 Odense, Denmark.
Jakob Appel
JA
Østergaard
Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark.
Esben
E
Axelgaard
Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark.
Gitte Krogh
GK
Nielsen
Department of Immunology, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan.
Yuichi
Y
Endo
Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark.
Steffen
S
Thiel
Department of Endocrinology and Internal Medicine, Aarhus University Hospital, 8000 Aarhus, Denmark.
Troels Krarup
TK
Hansen
D000418:Albumins / Q000378:metabolism; D000818:Animals; D003404:Creatinine / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003928:Diabetic Nephropathies / Q000235:genetics / Q000378:metabolism*; D005260:Female; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D037102:Lectins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D000097604:Ficolins
D000418:Albumins; D003404:Creatinine; D037102:Lectins; D000097604:Ficolins