Seasonal Variations of Metabolome and Tyrosinase Inhibitory Activity of Lespedeza maximowiczii during Growth Periods.
Lespedeza species are useful for pasture and energy crops as well as medical plants. We determined the metabolites discriminated from the each growth period (3, 4, 6, 8, 15, and 18 months) after germination in leaves and stems of Lespedeza maximowizii by a metabolomics technique. Specifically, levels of sugars and luteolin-dominated derivatives were significantly elevated in samples harvested in November. This may be related to the cold tolerance mechanism against the low temperatures of the winter season. The concentrations of secondary metabolites, isoflavones and flavanones, as well as tyrosinase inhibitory activity were the highest in the 6 month samples, which were harvested in September, during the fall season. The tyrosinase inhibitory activity in leaves was higher than that in stems irrespective of the growth period. This study suggests that mass spectrometry-based metabolite profiling could be used as a tool to examine quantitative or qualitative metabolite changes related to seasonal variations and to understand the correlation between activity and metabolites.
Department of Bioscience and Biotechnology, Konkuk University , 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
Na kyung
Nk
Kim
Department of Bioscience and Biotechnology, Konkuk University , 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
Hye Min
HM
Park
Department of Environment and Forest Resources, Chungnam National University , 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea.
Joongku
J
Lee
Division of Plant and Soil Sciences, West Virginia University , Morgantown, West Virginia 26505, United States.
Kang-Mo
KM
Ku
Department of Bioscience and Biotechnology, Konkuk University , 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
Choong Hwan
CH
Lee
D000363:Agaricales / Q000737:chemistry / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000032:analysis* / Q000378:metabolism; D029884:Lespedeza / Q000737:chemistry* / Q000254:growth & development* / Q000378:metabolism; D055442:Metabolome; D014442:Monophenol Monooxygenase / Q000032:analysis / Q000037:antagonists & inhibitors*; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism; D012621:Seasons
D004791:Enzyme Inhibitors; D010936:Plant Extracts; D014442:Monophenol Monooxygenase
Lespedeza maximowiczii; growth period; metabolite profiling; tyrosinase inhibitory activity