Single-Walled Carbon Nanohorns Inhibit Proliferation of Conjunctival Melanoma Cell Lines CRMM-1 and Involved in Energy Metabolism.
SWNHs induces accumulate with the levels of cytotoxic effects on different cell types and organs in animal models; it in a wide range may used for biomedical imaging. Conjunctival melanoma is a rare but potentially fatal ocular surface tumor. Recent studies of us have indicated the ability of SWNHs to inhibit proliferation of conjunctival melanoma cell line CRMM-1. But the role and molecular mechanisms of it was unclear. To address this question, the research about direct role of SWNHs on the growth, proliferation and apoptosis of CRMM-1 had been performed. Our results indicate that followed with the increasing concentrations of SWNHs, the number of cells decreased and apoptotic cells increased significantly. SWNHs delayed obviously mitotic entry of cells, and these effects followed the cultured time and the gradually increasing concentrations of SWNHs. SWNHs inhibited proliferation of cells at each time point in a time and dose-dependent manner, too. The TEM images showed that individual spherical SWNHs particles smaller than 100 nm in diameters were localized inside cytoplasma of cells. In summary, SWNHs inhibited mitotic entry, growth and proliferation of conjunctival melanoma cells and promoted its apoptosis, inhibited energy metabolism of cells in a dose-dependent manner. The roles of SWNHS on conjunctival melanoma cells were implicating energy metabolism. It may be the effective methods for treatment to conjunctival melanoma.
Yongmei
Y
Cao
Yan
Y
Zhang
Mingwei
M
Zhao
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D002244:Carbon / Q000008:administration & dosage* / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003230:Conjunctival Neoplasms / Q000188:drug therapy* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008422:Materials Testing; D008545:Melanoma / Q000188:drug therapy* / Q000378:metabolism*; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000648:ultrastructure; D010101:Oxygen Consumption / Q000187:drug effects; D010316:Particle Size; D016896:Treatment Outcome
D000970:Antineoplastic Agents; D002244:Carbon