Synthesis and Evaluation of DNA Based Quantum Dot Fluorescence In Situ Hybridization (FISH) Probe for Telomere Detection.
Efficient oligonucleotide probe design and synthesis based on polymer-coated CdSe/ZnS quantum dot (QD) is demonstrated for detection of telomeres in human monocyte and Leishmania major, a protozoan pathogenic parasite. The highly photoluminescent polymer-coated QDs conjugated with various length of telomere probe sequences were prepared via carbodiimide chemistry and characterized. Specific detection of telomere was observed when DNA sequence was (CCCAAT)n (n = 5 or 3) probe sequence, rather than (GGGTTA)n (n = 3, 5, 8). The sensitivity and specificity were comparable with commercially available PNA probe for human telomere detection.
Youngseon
Y
Choi
Jair Lage
JL
De Siqueira Neto
Carolina
C
Moraes
Lucio
L
Freitas
Rita
R
Song
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D018320:Leishmania major / Q000235:genetics*; D008422:Materials Testing; D008969:Molecular Sequence Data; D058726:Nanoconjugates / Q000737:chemistry; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D016615:Telomere / Q000235:genetics*
D015342:DNA Probes; D058726:Nanoconjugates; D004247:DNA