Methotrexate intercalated layered double hydroxides with the mediation of surfactants: Mechanism exploration and bioassay study.
Methotrexatum intercalated layered double hydroxides (MTX/LDHs) hybrids were synthesized by the co-precipitation method and three kinds of nonionic surfactants with different hydrocarbon chain lengths were used. The resulting hybrids were then characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and transmission electron microscopy (TEM). XRD and FTIR investigations manifest the successful intercalation of MTX anions into the interlayer of LDHs. TEM graphs indicate that the morphology of the hybrids changes with the variation of the chain length of the surfactants, i.e., the particles synthesized using polyethylene glycol (PEG-7) present regular disc morphology with good monodispersity, while samples with the mediation of alkyl polyglycoside (APG-14) are heavily aggregated and samples with the addition of polyvinylpyrrolidone (PVP-10) exhibit irregular branches. Furthermore, the release and bioassay experiments show that monodisperse MTX/LDHs present good controlled-release and are more efficient in the suppression of the tumor cells.
Jiangsu Key Laboratory of Biofunctional Material, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210023, China.
Chao-Fan
CF
Dai
Jiangsu Key Laboratory of Biofunctional Material, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210023, China.
De-Ying
DY
Tian
Jiangsu Key Laboratory of Biofunctional Material, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210023, China. Electronic address: lishuping@njnu.edu.cn.
Shu-Ping
SP
Li
Jiangsu Key Laboratory of Biofunctional Material, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210023, China.
Xiao-Dong
XD
Li
D065966:Absorption, Physicochemical; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D003692:Delayed-Action Preparations / Q000008:administration & dosage* / Q000138:chemical synthesis; D004058:Diffusion; D004357:Drug Synergism; D006801:Humans; D006878:Hydroxides / Q000737:chemistry*; D008727:Methotrexate / Q000008:administration & dosage* / Q000737:chemistry; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D013501:Surface-Active Agents / Q000737:chemistry*; D016896:Treatment Outcome
D000964:Antimetabolites, Antineoplastic; D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D006878:Hydroxides; D053769:Nanocapsules; D013501:Surface-Active Agents; D008727:Methotrexate
Bioassay experiment; Layered double hydroxides; Methotrexatum; Nonionic surfactants