Enterococcus bulliens sp. nov., a novel lactic acid bacterium isolated from camel milk.
Four lactic acid bacteria isolates obtained from fresh dromedary camel milk produced in Dakhla, a city in southern Morocco, were characterised in order to determine their taxonomic position. The four isolates had highly similar MALDI-TOF MS and RAPD fingerprints and identical 16S rRNA gene sequences. Comparative sequence analysis revealed that the 16S rRNA gene sequence of the four isolates was most similar to that of Enterococcus sulfureus ATCC 49903(T) and Enterococcus italicus DSM 15952(T) (99.33 and 98.59% similarity, respectively). However, sequence analysis of the phenylalanyl-tRNA synthase (pheS), RNA polymerase (rpoA) and ATP synthase (atpA) genes revealed that the taxon represented by strain LMG 28766(T) was well separated from E. sulfureus LMG 13084(T) and E. italicus LMG 22039(T), which was further confirmed by DNA-DNA hybridization values that were clearly below the species demarcation threshold. The novel taxon was easily differentiated from its nearest neighbour species through sequence analysis of protein encoding genes, MALDI-TOF mass spectrometry and multiple biochemical tests, but had a similar percentage G+C content of about 39%. We therefore propose to formally classify these isolates as Enterococcus bulliens sp. nov., with LMG 28766(T) (=CCMM B1177(T)) as the type strain.
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium.|Laboratoire de Biotechnologie Microbienne, Faculté des Sciences et Techniques de Fès-Saïs, Route d'Imouzzer, B.P. 2202, Fez, Morocco.
Zaina
Z
Kadri
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium.
Freek
F
Spitaels
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium.
Margo
M
Cnockaert
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium.
Jessy
J
Praet
Laboratoire de Biotechnologie Microbienne, Faculté des Sciences et Techniques de Fès-Saïs, Route d'Imouzzer, B.P. 2202, Fez, Morocco.
Omar
O
El Farricha
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium.
Jean
J
Swings
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium. peter.vandamme@ugent.be.
Peter
http://orcid.org/0000-0002-5581-7937
P
Vandamme
D000818:Animals; D001482:Base Composition; D002162:Camelus; D004269:DNA, Bacterial; D016983:Enterococcus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005516:Food Microbiology; D019344:Lactic Acid / Q000096:biosynthesis*; D008892:Milk / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid
Camel milk microbiota; Enterococcus bulliens sp. nov.; Fresh dromedary milk; Lactic acid bacteria; Taxonomy