A Deep Insight Into the Sialotranscriptome of the Chagas Disease Vector, Panstrongylus megistus (Hemiptera: Heteroptera).
Saliva of blood-sucking arthropods contains a complex cocktail of pharmacologically active compounds that assists feeding by counteracting their hosts' hemostatic and inflammatory reactions. Panstrongylus megistus (Burmeister) is an important vector of Chagas disease in South America, but despite its importance there is only one salivary protein sequence publicly deposited in GenBank. In the present work, we used Illumina technology to disclose and publicly deposit 3,703 coding sequences obtained from the assembly of >70 million reads. These sequences should assist proteomic experiments aimed at identifying pharmacologically active proteins and immunological markers of vector exposure. A supplemental file of the transcriptome and deducted protein sequences can be obtained from http://exon.niaid.nih.gov/transcriptome/P_megistus/Pmeg-web.xlsx.
National Institute of Allergy and Infectious Diseases, Laboratory of Malaria and Vector Research, 12735 Twinbrook Parkway, MD 20852. jribeiro@niaid.nih.gov.
José M C
JM
Ribeiro
Institute of Parasitology, Biology Centre of the Academy of Sciences of Czech Republic, CZ-370 05 Ceske Budejovice, Czech Republic.
Alexandra
A
Schwarz
National Institute of Allergy and Infectious Diseases, Laboratory of Malaria and Vector Research, 12735 Twinbrook Parkway, MD 20852.
Ivo M B
IM
Francischetti
D000818:Animals; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009758:Nymph / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010203:Panstrongylus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010802:Phylogeny; D040901:Proteomics; D012463:Saliva / Q000737:chemistry; D012471:Salivary Proteins and Peptides / Q000235:genetics* / Q000378:metabolism; D012795:Sialoglycoproteins / Q000235:genetics* / Q000378:metabolism
D019476:Insect Proteins; D012471:Salivary Proteins and Peptides; D012795:Sialoglycoproteins
Chagas disease; medical entomology; salivary gland; transcriptome; vector biology