A polysaccharide (PNPA) from Pleurotus nebrodensis offers cardiac protection against ischemia-reperfusion injury in rats.
In this study, we isolated a polysaccharide (PNPA), with a molecular weight of 105kDa, from the fruiting bodies of Pleurotus nebrodensis. It had a backbone consisting of 1,3-linked-d-glucpyranosyl and 1,3,6-linked-d-galactopyranosyl residues, which was terminated with 1-linked-d-mannopyranosyl terminal at O-3 position of 1,3,6-linked-d-galactopyranosyl unit along the main chain in the ratio of 4:1:1. We further examined the effect of PNPA on myocardial ischemia-reperfusion (I/R) injury in rats and elucidated the underlying mechanism. Pretreatment with PNPA (100 and 400mg/kg) for 30 days significantly attenuated myocardial infarct size as compared to I/R model group. A decrease in superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) levels, as well as an increased malondialdehyde (MDA) content were observed in both myocardial serum and tissues of control I/R group, whereas pretreatment with PNPA markedly restored these change, and also relieved myocardial cell apoptosis. These results suggested that PNPA achieved protective effect on myocardial I/R injury in part through improving endogenous antioxidants and suppressing myocardial cell apoptosis.
Department of Cardiology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China.
Bingju
B
Yan
Department of General Surgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China.
Liying
L
Jing
Department of General Surgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China. Electronic address: wangjunlnmu@126.com.
Jun
J
Wang
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects*; D006031:Glycosylation; D008297:Male; D008970:Molecular Weight; D009203:Myocardial Infarction / Q000150:complications / Q000517:prevention & control; D015428:Myocardial Reperfusion Injury / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D011134:Polysaccharides
Antioxidants; Cardioprotection; Ischemia–reperfusion injury; Oxidative stress; Pleurotus nebrodensis; Polysaccharide