Evaluation of DiversiLab®, MLST and PFGE typing for discriminating clinical Enterococcus faecium isolates.
We evaluated and critically assessed the performance and discriminatory power of a rep-PCR based commercial test DiversiLab® Enterococcus kit (bioMerieux) for typing a set of 65 representative isolates of Enterococcus faecium/VRE and compared it to state-of-the-art typing techniques such as PFGE and MLST.
Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.
Guido
G
Werner
Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.
Carola
C
Fleige
Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany; Institute for Microbiology, Ernst-Moritz-Arndt-University, Greifswald, Germany.
Bernd
B
Neumann
Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.
Jennifer K
JK
Bender
Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.
Franziska
F
Layer
Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.
Ingo
I
Klare
D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D016984:Enterococcus faecium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D058885:Multilocus Sequence Typing / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods
DiversiLab; Outbreak; Rep-PCR; Typing; VRE