Molecular Characterization of a Catalase-Negative Staphylococcus aureus Blood Culture Isolate.
Here we report a catalase-negative methicillin-sensitive Staphylococcus aureus isolate collected from a blood culture. Sequencing through the gene encoding catalase, katA, demonstrated a 2-bp insertion. The resulting frameshift mutation generates a protein that has lost 26 amino acids (aa) at its C-terminal domain.
National University Hospital, Department of Laboratory Medicine, Microbiology Unit, Singapore, Republic of Singapore Jeanette_Teo@nuhs.edu.sg.
Jeanette W P
http://orcid.org/0000-0002-9321-3341
JW
Teo
Novena Vascular and Varicose Vein Centre, Mount Elizabeth Novena Hospital, Singapore, Republic of Singapore.
Steven
S
Kum
National University Hospital, Department of Laboratory Medicine, Microbiology Unit, Singapore, Republic of Singapore.
Roland
R
Jureen
National University Hospital, Department of Laboratory Medicine, Microbiology Unit, Singapore, Republic of Singapore.
Raymond T P
RT
Lin
D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D016368:Frameshift Mutation; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D018384:Oxidative Stress / Q000502:physiology; D010544:Peroxidases / Q000235:genetics*; D019736:Prostheses and Implants / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D013530:Surgical Wound Infection / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010544:Peroxidases; C081610:catalase-peroxidase, bacteria