Mn-SOD 47 CC genotype in combination with high tea consumption may prevent complications in Tunisian type-2 diabetes.
Reactive oxygen species metabolizing enzymes may play an important role in the prevention of type-2 diabetes (T2D) complications. We analyzed the association between Cu/Zn-SOD +35 A/C, Mn-SOD T47C, and CAT -21 A/T gene polymorphisms and complications, in combination with tea consumption in Tunisian T2D. A sample of 366 T2D subjects was enrolled in this study. All participants were asked about tea consumption and frequency. Anthropometric, clinical, and routine biochemical characteristics were obtained from subjects' updated medical records. Malondialdehyde, as an early marker of lipid peroxidation, was measured in plasma samples. Urinary polyphenol derivatives (UPDs), as a marker of polyphenols intake, were assessed by the Folin-Ciocalteu assay. SODs and CAT genotypes were determined by conventional restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) methods. From all subjects, the results showed that in high tea consumers (>3 cups/day), the frequency of the Mn-SOD 47 CC genotype was significantly higher in T2D without complications compared with T2D with complications (P = 0.03; OR = 0.284; 95%CI = 0.086-0.939). However, no significant associations were observed with Cu/Zn-SOD +35 A/C or CAT -21 A/T genes polymorphisms. Additionally, the evaluation of UPDs showed that individuals carrying the Mn-SOD 47 CC genotype and consuming more than three cups of tea per day present significantly higher UPDs (P = 0.038). In conclusion, the Mn-SOD 47 C variant in combination with high tea consumption may provide protection against complications in T2D.
Research Unit on Antioxidant Compounds, Oxidative Stress, Trace Elements and Metabolic Diseases, High School of Health Sciences of Tunis, University of Tunis, El Manar, Tunis, Tunisia h.abaidi@yahoo.fr.
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Abaidi
Research Unit of Biology and Molecular Anthropology Applied to Development and Health, Faculty of Pharmacy of Monastir, Monastir, Tunisia.
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Denden
Research Unit on Antioxidant Compounds, Oxidative Stress, Trace Elements and Metabolic Diseases, High School of Health Sciences of Tunis, University of Tunis, El Manar, Tunis, Tunisia.
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Ghazouani
National Institute of Nutrition and Food Technology, Tunis, Tunisia.
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Trimèche
Research Unit on Antioxidant Compounds, Oxidative Stress, Trace Elements and Metabolic Diseases, High School of Health Sciences of Tunis, University of Tunis, El Manar, Tunis, Tunisia.
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Snoussi
Research Unit of Biology and Molecular Anthropology Applied to Development and Health, Faculty of Pharmacy of Monastir, Monastir, Tunisia.
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Haj Khelil
Research Unit of Biology and Molecular Anthropology Applied to Development and Health, Faculty of Pharmacy of Monastir, Monastir, Tunisia.
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Ben Chibani
Research Unit on Antioxidant Compounds, Oxidative Stress, Trace Elements and Metabolic Diseases, High School of Health Sciences of Tunis, University of Tunis, El Manar, Tunis, Tunisia.
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Hamdaoui
D002374:Catalase / Q000235:genetics; D048909:Diabetes Complications / Q000235:genetics* / Q000517:prevention & control; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D065840:Protective Factors; D013482:Superoxide Dismutase / Q000235:genetics*; D013662:Tea; D014416:Tunisia
D013662:Tea; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase