Evaluation of the smear layer removal and erosive capacity of EDTA, boric acid, citric acid and desy clean solutions: an in vitro study.
BACKGROUND
The purpose of this study was to investigate the smear layer removal and erosive capacity of various irrigation solutions with sequential use of NaOCl on instrumented root canal walls.


METHODS
The root canals of single-rooted teeth were instrumented with ProTaper rotary instrument. Then, the teeth were randomly divided into five experimental groups. The root canals were irrigated with one of the following solutions (5 mL/1 min): 5% EDTA, 5% boric acid (BA), a mixture of BA and CA, 2.5% citric acid (CA) and 5% Desy Clean. After irrigating with 2.5% NaOCl and distilled water, the roots were split into two halves and each half was prepared for SEM examination. Representative photographs were taken from each third at x500 and x1000 magnifications. Double blind scoring was performed by two calibrated observers for smear layer and erosion. The scores were statistically analyzed using Kruskal-Wallis, Dunn's post hoc and Spearman's correlation tests (p = 0.05).


RESULTS
There were statistically significant differences among the solutions by means of smear layer and erosion (p < 0.05). While 2.5% CA solution was the most effective solution in removal of smear layer, it was also the most erosive solution (p < 0.05). 5% Desy Clean removed smear layer effectively and caused less erosion. There was a negative, but statistically significant correlation between presence of smear layer and erosion (r = -0.684; p < 0.0001).


CONCLUSIONS
Desy Clean can be a promising agent as an irrigation solution with optimal smear layer removal capacity and less erosive effects.
Department of Endodontology, Ege University, School of Dentistry, 35100, Izmir, Turkey. btugbaturk@gmail.com.
Tugba
T
Turk
Department of Endodontology, Ege University, School of Dentistry, 35100, Izmir, Turkey. mehmetkaval@hotmail.com.
Mehmet Emin
ME
Kaval
Private Practise, İzmir, Turkey. bhsen@yahoo.com.
Bilge Hakan
BH
Şen
D019342:Acetic Acid / Q000494:pharmacology; D001888:Boric Acids / Q000494:pharmacology; D019343:Citric Acid / Q000494:pharmacology; D003786:Dental Pulp Cavity / Q000187:drug effects* / Q000648:ultrastructure; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D004338:Drug Combinations; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010781:Photography / Q000379:methods; D011897:Random Allocation; D012388:Root Canal Irrigants / Q000494:pharmacology*; D018915:Root Canal Preparation / Q000379:methods; D016369:Smear Layer / Q000188:drug therapy* / Q000473:pathology; D020160:Sodium Benzoate / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013011:Sorbic Acid / Q000494:pharmacology; D014077:Tooth Erosion / Q000209:etiology* / Q000473:pathology
D001888:Boric Acids; C000613087:Desy Clean; D004338:Drug Combinations; D012388:Root Canal Irrigants; D019343:Citric Acid; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D020160:Sodium Benzoate; D019342:Acetic Acid; C032688:boric acid; D013011:Sorbic Acid