Copy-out-Paste-in Transposition of IS911: A Major Transposition Pathway.
IS911 has provided a powerful model for studying the transposition of members of a large class of transposable element: the IS3 family of bacterial Insertion Sequences (IS). These transpose by a Copy-out-Paste-in mechanism in which a double-strand IS circle transposition intermediate is generated from the donor site by replication and proceeds to integrate into a suitable double strand DNA target. This is perhaps one of the most common transposition mechanisms known to date. Copy-out-Paste-in transposition has been adopted by members of at least eight large IS families. This chapter details the different steps of the Copy-out-Paste-in mechanism involved in IS911 transposition. At a more biological level it also describes various aspects of regulation of the transposition process. These include transposase production by programmed translational frameshifting, transposase expression from the circular intermediate using a specialized promoter assembled at the circle junction and binding of the nascent transposase while it remains attached to the ribosome during translation (co-translational binding). This co-translational binding of the transposase to neighboring IS ends provides an explanation for the longstanding observation that transposases show a cis-preference for their activities.
Laboratoire de Microbiologie et Génétique Moléculaires, CNRS, Toulouse, France.
Michael
M
Chandler
Laboratoire de Microbiologie et Génétique Moléculaires, CNRS, Toulouse, France.
Olivier
O
Fayet
Laboratoire de Microbiologie et Génétique Moléculaires, CNRS, Toulouse, France.
Philippe
P
Rousseau
Laboratoire de Microbiologie et Génétique Moléculaires, CNRS, Toulouse, France.
Bao
B
Ton Hoang
Laboratoire de Microbiologie et Génétique Moléculaires, CNRS, Toulouse, France.
Guy
G
Duval-Valentin
D001419:Bacteria / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004251:DNA Transposable Elements; D011995:Recombination, Genetic; D019895:Transposases / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D019895:Transposases