RAPD-inferred genetic variability of some indigenous Rhizobium leguminosarum isolates from red clover (Trifolium pratense L.) nodules.
The application of commercial rhizobial inoculants to legume crops is proving to be an alternative to synthetic fertilizer use. The challenge for sustainable agriculture resides in the compatibility between crop, inoculants and environmental conditions. The evaluation of symbiotic efficiency and genetic diversity of indigenous rhizobial strains could lead to the development of better inoculants and increased crop production. The genetic variability of 32 wild indigenous rhizobial isolates was assessed by RAPD (Random Amplified Polymorphic DNA). The strains were isolated from red clover (Trifolium pratense L.) nodules from two distinct geographical regions of Northern and Eastern Romania. Three decamer primers were used to resolve the phylogenetic relationships between the investigated isolates. Cluster analysis revealed a high diversity; most strains clustered together based on their geographical location.
Faculty of Biology, "Alexandru Ioan Cuza" University of Iaşi , Iaşi , Romania.
Andrei
A
Stefan
Craita M
CM
Rosu
Catalina
C
Stedel
Lucian D
LD
Gorgan
Rodica C
RC
Efrose
D014644:Genetic Variation; D019105:Random Amplified Polymorphic DNA Technique; D016961:Rhizobium leguminosarum / Q000235:genetics*; D013559:Symbiosis / Q000502:physiology*; D029921:Trifolium / Q000382:microbiology*
RAPD; Rhizobium leguminosarum; genetic variability; indigenous strains; red clover