Isolation and characterization of novel polymorphic microsatellite loci in large yellow croaker (Larimichthys crocea).
The large yellow croaker (Larimichthys crocea) is one of the largest marine net-cage cultured species in the oceans around China. In the present study, we isolated and characterized 13 polymorphic microsatellite markers from genomic libraries of L. crocea. Loci were screened for 10 wild specimens from 2 sites in southeast of China. All loci were polymorphic. The number of alleles per locus ranged from 2 to 21. The expected heterozygosity ranged from 0.233 to 0.838 and observed heterozygosity ranged from 0.527 to 0.935. Eleven loci were highly informative (polymorphic information content >0.5). Significant deviation from Hardy-Weinberg equilibrium was observed at 3 loci after Bonferroni's correction. The microsatellite loci may be valuable tools for studying the genetic diversity and genetic structure for conservation planning of the fish.
College of Animal Science and Technology, Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Hunan Agriculture University, Changsha, Hunan, China.
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XQ
Wang
College of Animal Science and Technology, Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Hunan Agriculture University, Changsha, Hunan, China.
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D
Zeng
College of Animal Science and Technology, Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Hunan Agriculture University, Changsha, Hunan, China 18maxiao@163.com.
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X
Ma
College of Animal Science and Technology, Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Hunan Agriculture University, Changsha, Hunan, China.
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G
Xiong
College of Animal Science and Technology, Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Hunan Agriculture University, Changsha, Hunan, China.
Z Y
ZY
Wang
Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo, Japan.
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T
Sakamoto
D000818:Animals; D056426:Genetic Loci; D018895:Microsatellite Repeats; D010473:Perciformes / Q000235:genetics*; D011110:Polymorphism, Genetic