A Cathepsin-L is required for invasive behavior during Air Sac Primordium development in Drosophila melanogaster.
The Drosophila Air Sac Primordium (ASP) has emerged as an important structure where cellular, genetic and molecular events responsible for invasive behavior and branching morphogenesis can be studied. In this report we present data which demonstrate that a Cathepsin-L encoded by the gene CP1 in Drosophila is necessary for invasive behavior during ASP development. We find that CP1 is expressed in ASP and knockdown of CP1 results in suppression of migratory and invasive behavior observed during ASP development. We further show that CP1 possibly regulates invasive behavior by promoting degradation of Basement Membrane. Our data provide clues to the possible role of Cathepsin L in human lung development and tumor invasion, especially, given the similarities between human lung and Drosophila ASP development.
Department of Biology and Biotechnology Center, Western Kentucky University, 1906 College Heights Boulevard, TCCW 351, Bowling Green, KY 42101, USA.
Qian
Q
Dong
Department of Biology and Biotechnology Center, Western Kentucky University, 1906 College Heights Boulevard, TCCW 351, Bowling Green, KY 42101, USA.
Breanna
B
Brenneman
Department of Biology and Biotechnology Center, Western Kentucky University, 1906 College Heights Boulevard, TCCW 351, Bowling Green, KY 42101, USA.
Christopher
C
Fields
Department of Biology and Biotechnology Center, Western Kentucky University, 1906 College Heights Boulevard, TCCW 351, Bowling Green, KY 42101, USA. Electronic address: ajay.srivastava@wku.edu.
Ajay
A
Srivastava
D000400:Air Sacs / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D001485:Basement Membrane / Q000254:growth & development / Q000378:metabolism; D002448:Cell Adhesion / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007814:Larva / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D034622:RNA Interference
D029721:Drosophila Proteins; D049452:Green Fluorescent Proteins; D003546:Cysteine Endopeptidases; C106944:Cysteine proteinase-1, Drosophila
Air Sac Primordium; Basement Membrane; Cathepsin-L; Cysteine Protease; Drosophila; Imaginal disc; Invasive behavior; Lung development