UCP2 protects against amyloid beta toxicity and oxidative stress in primary neuronal culture.
AD is a common neurodegenerative disease characterized by aggregated amyloid-beta (Aβ) peptide, and oxidative stress, while uncoupling protein 2 (UCP2) is a member of the anion carrier family, predicted the existence of a protein-regulated proton leak with the main purpose of controlling mitochondrial oxidative stress, reduce the generation of superoxide anion. we use the primary hippocampal neurons and add the different doses of Aβ1-40, then observe the change of UCP2 at different concentrations of Aβ, activity of LDH and the content of NO. Our results provide novel insight that UCP2 may protect hippocampal neurons exposed to amyloid β protein through decreasing ROS production. 20μmol/L Aβ1-40 significantly increased the activity of LDH and the content of NO. According to the correlation analysis, NO was significantly correlation with LDH, and UCP2 was significantly correlation with NO. These results suggest the potential of UCP2 as a therapeutic candidate for treating neurodegenerative diseases such as AD.
Department of Pathology, Dalian Medical University, Dalian, China.
Zhang
Z
Jun
Department of Pathology, Dalian Medical University, Dalian, China.
M M
MM
Ibrahim
Department of Physiology, Dalian Medical University, Dalian, China.
Gong
G
Dezheng
Department of Physiology, Dalian Medical University, Dalian, China.
Yuan
Y
Bo
Department of Physiology, Dalian Medical University, Dalian, China.
Wu
W
Qiong
Department of Physiology, Dalian Medical University, Dalian, China. Electronic address: dlcom@126.com.
Zou
Z
Yuan
D000544:Alzheimer Disease / Q000503:physiopathology*; D016229:Amyloid beta-Peptides / Q000008:administration & dosage*; D000818:Animals; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism*; D009474:Neurons / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress; D010446:Peptide Fragments / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D000071246:Uncoupling Protein 2
D016229:Amyloid beta-Peptides; D007473:Ion Channels; D024101:Mitochondrial Proteins; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; C000604391:Ucp2 protein, rat; D000071246:Uncoupling Protein 2; C078592:amyloid beta-protein (1-40); D009569:Nitric Oxide; D007770:L-Lactate Dehydrogenase
Alzheimer; Amyloid-beta protein; LDH; NO; Uncoupling protein 2; ’s disease