The Use and Effectiveness of Triple Multiplex System for Coding Region Single Nucleotide Polymorphism in Mitochondrial DNA Typing of Archaeologically Obtained Human Skeletons from Premodern Joseon Tombs of Korea.
Previous study showed that East Asian mtDNA haplogroups, especially those of Koreans, could be successfully assigned by the coupled use of analyses on coding region SNP markers and control region mutation motifs. In this study, we tried to see if the same triple multiplex analysis for coding regions SNPs could be also applicable to ancient samples from East Asia as the complementation for sequence analysis of mtDNA control region. By the study on Joseon skeleton samples, we know that mtDNA haplogroup determined by coding region SNP markers successfully falls within the same haplogroup that sequence analysis on control region can assign. Considering that ancient samples in previous studies make no small number of errors in control region mtDNA sequencing, coding region SNP analysis can be used as good complimentary to the conventional haplogroup determination, especially of archaeological human bone samples buried underground over long periods.
Bioanthropology and Paleopathology Lab, Institute of Forensic Science, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul 110-799, Republic of Korea ; Department of Anatomy, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul 110-799, Republic of Korea.
Chang Seok
CS
Oh
Bioanthropology and Paleopathology Lab, Institute of Forensic Science, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul 110-799, Republic of Korea ; Department of Forensic Medicine, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul 110-799, Republic of Korea.
Soong Deok
SD
Lee
Department of Anatomy, Ewha Womans University School of Medicine, 911-1 Mok-6-dong, Yangcheon-Gu, Seoul 158-710, Republic of Korea.
Yi-Suk
YS
Kim
Bioanthropology and Paleopathology Lab, Institute of Forensic Science, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul 110-799, Republic of Korea ; Department of Anatomy, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul 110-799, Republic of Korea.
Dong Hoon
DH
Shin
D044466:Asian People / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D005269:Femur; D018732:Forensic Anthropology; D005819:Genetic Markers; D049669:History, 16th Century; D049670:History, 17th Century; D049671:History, 18th Century; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D056910:Republic of Korea
D004272:DNA, Mitochondrial; D005819:Genetic Markers