Shear Wave Elastography for Assessment of Steatohepatitis and Hepatic Fibrosis in Rat Models of Non-Alcoholic Fatty Liver Disease.
The purpose of this study was to evaluate shear wave elastography (SWE) as a method for determining the severity of non-alcoholic fatty liver disease (NAFLD) and the stage of hepatic fibrosis, as well as the major determinants of liver elasticity among the various histologic and biomolecular changes associated with NAFLD. Rat NAFLD models with various degrees of NAFLD severity were created and imaged using SWE. The explanted livers were subjected to histopathologic evaluation and RNA expression analysis. Among the histologic and biomolecular findings, the fibrosis stage and the collagen RNA level were significant independent factors associated with liver elasticity (p < 0.001). Liver elasticity was effective in detecting non-alcoholic steatohepatitis (NASH) and in determining fibrosis stage, and the corresponding areas under the receiver operating characteristic curves were 0.963 and 0.927-0.997, respectively. In conclusion, SWE is a potential non-invasive method for the detection of NASH and staging of hepatic fibrosis in patients with NAFLD.
Department of Radiology and Research Institute of Radiology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea; Department of Radiology, Hanyang University Medical Center, Seoul, Korea.
Bo-Kyeong
BK
Kang
Department of Radiology and Research Institute of Radiology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea. Electronic address: seungsoolee@amc.seoul.kr.
Seung Soo
SS
Lee
Department of Radiology and Research Institute of Radiology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
Hyunhee
H
Cheong
Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
Seung Mo
SM
Hong
Department of Pathology, Hanyang University Medical Center, Seoul, Korea.
Kiseok
K
Jang
Department of Radiology and Research Institute of Radiology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
Moon-Gyu
MG
Lee
D000818:Animals; D004195:Disease Models, Animal; D054459:Elasticity Imaging Techniques; D005234:Fatty Liver / Q000000981:diagnostic imaging*; D008099:Liver / Q000000981:diagnostic imaging; D008103:Liver Cirrhosis / Q000000981:diagnostic imaging*; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000000981:diagnostic imaging*; D012372:ROC Curve; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index
Hepatic fibrosis; Liver elasticity; Nonalcoholic fatty liver disease; Nonalcoholic steatohepatitis; Shear wave elastography