Analysis of genetic diversity identified by amplified fragment length polymorphism marker in hybrid wheat.
Amplified fragment length polymorphism markers were used to assess genetic diversity in 10 male sterile wheat crop lines (hetero-cytoplasm with the same nucleus) in relation to a restorer wheat line. These male sterile lines were evaluated using 64 amplified fragment length polymorphism primer combinations, and 13 primers produced polymorphic bands, generating a total 682 fragments. Of the 682 fragments, 113 were polymorphic. The polymorphic information content and marker index values demonstrated the utility of the primer combinations used in the present study. Unweighted pair group method with arithmetic mean and principal coordinate analysis of the genotypic data revealed clustering of accessions based on genetic relationships, and accessions were separated into 2 groups with their restorer line. Jaccard's similarity coefficient values suggested good variability among the male sterile lines, indicating their utility in breeding programs. The fallouts of analysis of molecular variance showed large within-group population variation, accounting for 77% of variation, while among-group comparison accounted for 23% of the total molecular variation, which was statistically significant. The molecular diversity observed in this study will be useful for selecting appropriate accessions for plant improvement and hybridization through molecular-breeding approaches and for developing suitable conservation strategies.
Key Laboratory of Crop Heterosis, College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Center, Wheat Breeding Engineering Research Center, Ministry of Education, Shaanxi Province, Yangling, China.
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Ejaz
Key Laboratory of Crop Heterosis, College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Center, Wheat Breeding Engineering Research Center, Ministry of Education, Shaanxi Province, Yangling, China.
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Qidi
Key Laboratory of Crop Heterosis, College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Center, Wheat Breeding Engineering Research Center, Ministry of Education, Shaanxi Province, Yangling, China.
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Gaisheng
Key Laboratory of Crop Heterosis, College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Center, Wheat Breeding Engineering Research Center, Ministry of Education, Shaanxi Province, Yangling, China.
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Na
Key Laboratory of Crop Heterosis, College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Center, Wheat Breeding Engineering Research Center, Ministry of Education, Shaanxi Province, Yangling, China.
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Huiyan
Key Laboratory of Crop Heterosis, College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Center, Wheat Breeding Engineering Research Center, Ministry of Education, Shaanxi Province, Yangling, China.
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Qunzhu
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods; D000704:Analysis of Variance; D002678:Chimera / Q000235:genetics; D016000:Cluster Analysis; D017931:DNA Primers; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D000069600:Plant Breeding; D014908:Triticum / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers