Myelin ultrastructure of sciatic nerve in rat experimental autoimmune neuritis model and its correlation with associated protein expression.
To explore the relationship of peripheral nerve ultrastructure and its associated protein expression in experimental autoimmune neuritis (EAN). EAN was established in Lewis rats using an emulsified mixture of P0 peptide 180-199, Mycobacterium tuberculosis, and incomplete Freund's adjuvant. Rats immunized with saline solution were used as a control group. Sciatic nerve ultrastructure and immunofluorescence histopathology were measured at the neuromuscular severity peak on day 18 post-induction. Cell-specific protein markers were used for immunofluorescence histopathology staining to characterize sciatic nerve cells: CD3 (T cell), Iba-1 (microglia), S100 (myelin), and neurofilament 200 (axon). The results showed that swelling of the myelin lamellae, vesicular disorganization, separation of the myelin lamellae, and an attenuation or disappearance of the axon were observed by transmission electron microscopy in the EAN group. CD3 and Iba-1 increased significantly in the structures characterized by separation or swelling of the myelin lamellae, and increased slightly in the structures characterized by vesicular of the myelin lamellae, S100 decreased in the structures characterized by vesicular disorganization or separation of the myelin lamellae. And neurofilament 200 decreased in the structures characterized by separation of the myelin lamellae. Furthermore, we found that Iba1 were positive in the myelin sheath, and overlapped with S100, which significantly indicated that Schwann cells played as macrophage-like cells during the disease progression of ENA. Our findings may be a significant supplement for the knowledge of EAN model, and may offer a novel sight on the treatment of Guillain-Barré syndrome.
Department of Rehabilitation, The Second Hospital, Shandong University Shan Dong, China ; Taian City Central Hospital Shan Dong, China.
Xiao-Jing
XJ
Yuan
Department of Tissue Engineering, China Medical University Shenyang, Liaoning, China.
Yu-Jun
YJ
Wei
Department of Tissue Engineering, China Medical University Shenyang, Liaoning, China.
Qiang
Q
Ao
Institute of Neurological Disorders, Yuquan Hospital, Tsinghua University Beijing, China.
Kai
K
Gong
Department of Tissue Engineering, China Medical University Shenyang, Liaoning, China.
Jian-Yong
JY
Wang
Department of Rehabilitation, The Second Hospital, Shandong University Shan Dong, China.
Qiang-San
QS
Sun
Department of Ophthalmology, UCSF USA.
Ling
L
Zhang
Taian City Central Hospital Shan Dong, China.
Zun-Cheng
ZC
Zheng
Institute of Neurological Disorders, Yuquan Hospital, Tsinghua University Beijing, China.
Lin
L
Chen
D000818:Animals; D000831:Animals, Newborn; D005620:Freund's Adjuvant; D006801:Humans; D007114:Immunization; D008055:Lipids; D008264:Macrophages / Q000378:metabolism; D008297:Male; D009186:Myelin Sheath / Q000378:metabolism; D009444:Neuritis, Autoimmune, Experimental / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D012584:Sciatic Nerve / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology
D008055:Lipids; C114843:incomplete Freund's adjuvant; D005620:Freund's Adjuvant
Experimental autoimmune neuritis (EAN); immunofluorescence histopathology; myelin ultrastructure; protein marker; sciatic nerve