Enniatin A1, enniatin B1 and beauvericin on HepG2: Evaluation of toxic effects.
Hepatotoxicity of three Fusarium mycotoxins, beauvericin (BEA) and two enniatins (ENNs) ENN A1 and ENN B1, in hepatocarcinoma cells (HepG2) were evaluated and compared. Concentrations used were 1.5 and 3 μM at 24, 48 and 72 h for each mycotoxin. Flow cytometry was used to examine enniatins effects on cell proliferation, to characterize the cell cycle phase where the cells blocked and to study the mitochondria role in ENNs-induced apoptosis. ENN B1 treated cells showed a time dependent G1 blockade at both concentrations used. ENN A1 and BEA decreased the apoptotic-necrotic percentage of cells comparing to control and disrupted the MMP as observed by TMRM and ToPro-3 fluorochromes signal. It is proposed a decreasing mycotoxin order by number of effects as follows: BEA > ENN B1 > ENN A1, with 47, 20 and 16%, respectively out of all situations compared.
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, Burjassot, València 46100, Spain. Electronic address: ana.juan@uv.es.
Ana
A
Juan-García
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, Burjassot, València 46100, Spain.
María-José
MJ
Ruiz
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, Burjassot, València 46100, Spain.
Guillermina
G
Font
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, Burjassot, València 46100, Spain.
Lara
L
Manyes
D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D047630:Depsipeptides / Q000633:toxicity*; D005670:Fusarium / Q000378:metabolism; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D007700:Kinetics; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009183:Mycotoxins / Q000633:toxicity*; D009336:Necrosis; D013237:Stereoisomerism
D047630:Depsipeptides; D009183:Mycotoxins; C100264:enniatins; C004456:beauvericin
Beauvericin; Cell cycle; Enniatins; Flow cytomtery; Mitochondrial membrane potential; Mycotoxins