Three new milbemycins from a genetically engineered strain S. avermitilis MHJ1011.
Three new β-class milbemycins, 13α-hydroxy-4-ethy1 milbemycin β3 (1), 13α-hydroxy-25-ethy1 milbemycin β3 (2), 13α-hydroxy milbemycin β3 (3), were isolated from the broth of the genetically engineered strains Streptomyces avermitilis MHJ1011, whose aveA1 gene was replaced by milA1 gene seamlessly. Their structures were determined on the basis of extensive spectroscopic analysis and comparison with data from the literature. These three compounds, especially compound 1, exhibited potent acaricidal activity.
Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology, Zhejiang Agricultural and Forestry University, Lin'An, China.
Jun-jie
JJ
Pan
Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology, Zhejiang Agricultural and Forestry University, Lin'An, China.
Xu
X
Wan
Department of New Drug Screening, Zhejiang Hisun Pharmaceutical Co., Ltd., Taizhou, China.
Hui
H
Zhang
Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology, Zhejiang Agricultural and Forestry University, Lin'An, China.
Zhen
Z
Chen
Department of New Drug Screening, Zhejiang Hisun Pharmaceutical Co., Ltd., Taizhou, China.
Jun
J
Huang
Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology, Zhejiang Agricultural and Forestry University, Lin'An, China.
Bo
B
Yang
Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology, Zhejiang Agricultural and Forestry University, Lin'An, China.
An-liang
AL
Chen
Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology, Zhejiang Agricultural and Forestry University, Lin'An, China.|Department of New Drug Screening, Zhejiang Hisun Pharmaceutical Co., Ltd., Taizhou, China.
Ji-dong
JD
Wang
D056810:Acaricides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001681:Biological Assay; D003470:Culture Media / Q000737:chemistry; D018942:Macrolides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008958:Models, Molecular; D015394:Molecular Structure; D021261:Parasitic Sensitivity Tests; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D016019:Survival Analysis; D040102:Tetranychidae / Q000187:drug effects / Q000502:physiology
D056810:Acaricides; D003470:Culture Media; D018942:Macrolides; C027837:moxidectin