Molecular cloning and characterization of the light-harvesting chlorophyll a/b gene from the pigeon pea (Cajanus cajan).
Light-harvesting chlorophyll a/b-binding proteins (LHCB) have been implicated in the stress response. In this study, a gene encoding LHCB in the pigeon pea was cloned and characterized. Based on the sequence of a previously obtained 327 bp Est, a full-length 793 bp cDNA was cloned using the rapid amplification of cDNA ends (RACE) method. It was designated CcLHCB1 and encoded a 262 amino acid protein. The calculated molecular weight of the CcLHCB1 protein was 27.89 kDa, and the theoretical isoelectric point was 5.29. Homology search and sequence multi-alignment demonstrated that the CcLHCB1 protein sequence shared a high identity with LHCB from other plants. Bioinformatics analysis revealed that CcLHCB1 was a hydrophobic protein with three transmembrane domains. By fluorescent quantitative real-time polymerase chain reaction (PCR), CcLHCB1 mRNA transcripts were detectable in different tissues (leaf, stem, and root), with the highest level found in the leaf. The expression of CcLHCB1 mRNA in the leaves was up-regulated by drought stimulation and AM inoculation. Our results provide the basis for a better understanding of the molecular organization of LCHB and might be useful for understanding the interaction between plants and microbes in the future.
Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region and Ministry of Education, Institute of Agro-bioengineering, Guizhou University, Guiyang, 550025, China.
Guang
G
Qiao
Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region and Ministry of Education, Institute of Agro-bioengineering, Guizhou University, Guiyang, 550025, China. xpwen0121@aliyun.com.
Xiao-Peng
XP
Wen
Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region and Ministry of Education, Institute of Agro-bioengineering, Guizhou University, Guiyang, 550025, China.
Ting
T
Zhang
D000595:Amino Acid Sequence; D036561:Cajanus / Q000235:genetics*; D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D003001:Cloning, Molecular; D019295:Computational Biology; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data
D045342:Light-Harvesting Protein Complexes; D002734:Chlorophyll; C037184:chlorophyll b; D000077194:Chlorophyll A
Bioinformatics analysis; Gene expression; Light-harvesting chlorophyll a/b gene; Pigeon pea (Cajanus cajan); RACE