Efficacy of Mentha spicata essential oil in suppression of Aspergillus flavus and aflatoxin contamination in chickpea with particular emphasis to mode of antifungal action.
The present study reports in vivo antifungal and antiaflatoxigenic efficacy of Mentha spicata essential oil (EO) against toxigenic Aspergillus flavus strain LHP(C)-D6 in chickpea food system up to 12 months of storage. In addition, the mode of antifungal action of EO was also determined to understand the mechanism of fungal growth inhibition. The in vivo study with different concentrations of M. spicata EO showed dose-dependent decrease in fungal colony count as well as aflatoxin B1 concentration. The EO caused >50% protection in inoculated sets and >70% protection in uninoculated sets of chickpea food system against A. flavus at 1.0 μL mL(-1) air concentration. However, at the same concentration, EO caused 100% inhibition to aflatoxin B1 production in both sets when analyzed through high-performance liquid chromatography (HPLC). The antifungal target of EO in fumigated cells of A. flavus was found to be the plasma membrane when analyzed through electron microscopic observations and ions leakage test. The EO fumigated chickpea seeds showed 100% seed germination and seedling growth after 12 months of storage. Based on these observations, M. spicata EO can be recommended as plant-based preservative for safe protection of food commodities during storage conditions against fungal and most importantly mycotoxin contaminations.
Department of Botany, Gauhati University, Guwahati, 781014, Assam, India.
Akash
A
Kedia
Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005, UP, India.
Abhishek Kumar
AK
Dwivedy
Department of Botany, Gauhati University, Guwahati, 781014, Assam, India.
Dhruva Kumar
DK
Jha
Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005, UP, India. nkdubey2@rediffmail.com.
Nawal Kishore
NK
Dubey
D000348:Aflatoxins / Q000378:metabolism; D000935:Antifungal Agents / Q000494:pharmacology*; D001231:Aspergillus flavus / Q000187:drug effects* / Q000378:metabolism / Q000472:pathogenicity; D029863:Cicer / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology*; D005506:Food Contamination / Q000517:prevention & control; D005519:Food Preservation / Q000379:methods; D018525:Germination / Q000187:drug effects; D031337:Mentha spicata / Q000737:chemistry*; D009822:Oils, Volatile / Q000494:pharmacology*; D010938:Plant Oils / Q000494:pharmacology; D012639:Seeds / Q000187:drug effects
D000348:Aflatoxins; D000935:Antifungal Agents; D009822:Oils, Volatile; D010938:Plant Oils
Aflatoxin; Aspergillus flavus; Essential oil; In vivo; Mentha spicata