MCI extraction from Turkish galls played protective roles against X-ray-induced damage in AHH-1 cells.
OBJECTIVE
To investigate the protective effects of MCI extract from Turkish galls against apoptosis induced by X-ray radiation in the AHH-1.


METHODS
The cells were divided into: control group; X-ray radiation group; MCI group, in which the confluent cells were preincubated with 5 μg/ml MCI for 2 h followed by radiation. For the radiation, cells preincubated with MCI were exposed to X-ray beams with a dose of 8 Gy in total. Cell viability, apoptosis and intracellular alteration of redox were monitored by MTT and flow cytometry.


RESULTS
Compared with radiation group, the number of cells arrested at the G0/G1 phase was significantly reduced in MCI group (P < 0.05). X-ray radiation induces remarkable apoptosis in AHH-1, which was reversed by MCI. Compared with the radiation group, the generation of intracellular reactive oxygen species (ROS) was abrogated by pre-incubation with MCI (P < 0.05). In addition, the up-regulation of procaspase-3 induced by radiation was reversed by MCI. Radiation could induce up-regulation of Bax and down-regulation of Bcl-2; however, it is reversed completely after administration of MCI. Further, the enhanced expression of ERK and JNK induced by radiation was reversed by MCI.


CONCLUSIONS
MCI extract from Turkish galls played protective effects on the X-ray induced damage through enhancing the scavenging activity of ROS, decreasing Bax/Bcl-2 ratio and the down-regulating the activity of procaspase-3, as well as modulating the mitogen-activated protein kinase (MAPK) signaling pathways.
The First Affiliated Hospital, Xinjiang Medical University Urumqi 830011, China.
Jianhua
J
Yang
College of Pharmacy, Xinjiang Medical University Urumqi 830011, China.
Yan
Y
Zhou
Affiliated Tumor Hospital, Xinjiang Medical University Urumqi 830011, China.
Huibin
H
Liu
The First Affiliated Hospital, Xinjiang Medical University Urumqi 830011, China.
Jianhua
J
Wang
College of Pharmacy, Xinjiang Medical University Urumqi 830011, China.
Junping
J
Hu
D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D019610:Cytoprotection; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D010766:Phosphorylation; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D029963:Quercus / Q000737:chemistry; D011837:Radiation-Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D014965:X-Rays
D051017:Apoptosis Regulatory Proteins; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D020928:Mitogen-Activated Protein Kinases
MAPK signal pathway; Turkish galls; apoptosis; radiation