The expression characteristics of mt-ND2 gene in chicken.
Subunit 2 of NADH dehydrogenase (ND2) is encoded by the mt-ND2 gene and plays a critical role in controlling the production of the mitochondrial reactive oxygen species. Our study focused on exploring the mt-ND2 tissue expression patterns and the effects of energy restriction and dietary fat (linseed oil, corn oil, sesame oil or lard) level (2.5% and 5%) on its expression in chicken. The results showed that mt-ND2 gene was expressed in the 15 tissues of hybrid chickens with the highest level in heart and lowest level in pancreas tissue; 30% energy restriction did not significantly affect mt-ND2 mRNA level in chicken liver tissue. Both the mt-ND2 mRNA levels in chicken pectoralis (p < 0.05) and hepatic tissues (p < 0.05) at 42 d-old were affected by the type of dietary fats in 5% level, while not in abdominal fat tissues. The expression of mt-ND2 in hepatic tissues was down-regulated with chicken age (p < 0.01). The interactive effect of dietary fat types with chicken age (p < 0.05) was significant on mt-ND2 mRNA level. The study demonstrated that mt-ND2 gene was extensively expressed in tissues, and the expression was affected by dietary fat types and chicken age.
a College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University , Zhengzhou, Henan , China and.
Wenwen
W
Zhang
b Animal Science College, Sichuan Agricultural University , Ya'an, Sichuan China.
Lingling
L
Hou
a College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University , Zhengzhou, Henan , China and.
Ting
T
Wang
a College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University , Zhengzhou, Henan , China and.
Weiwei
W
Lu
a College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University , Zhengzhou, Henan , China and.
Yafei
Y
Tao
a College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University , Zhengzhou, Henan , China and.
Wen
W
Chen
b Animal Science College, Sichuan Agricultural University , Ya'an, Sichuan China.
Xiaohui
X
Du
a College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University , Zhengzhou, Henan , China and.
Yanqun
Y
Huang
D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage; D005260:Female; D015870:Gene Expression; D008099:Liver / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology; D009245:NADH Dehydrogenase / Q000235:genetics* / Q000378:metabolism
D030161:Avian Proteins; D004041:Dietary Fats; D024101:Mitochondrial Proteins; D009245:NADH Dehydrogenase
Chicken; dietary fat; energy restriction, mt-ND2; tissue expression pattern