Magnetic molecularly imprinted polymer nanoparticles based electrochemical sensor for the measurement of Gram-negative bacterial quorum signaling molecules (N-acyl-homoserine-lactones).
We have developed a novel and economical electrochemical sensor to measure Gram-negative bacterial quorum signaling molecules (AHLs) using magnetic nanoparticles and molecularly imprinted polymer (MIP) technology. Magnetic molecularly imprinted polymers (MMIPs) capable of selectively absorbing AHLs were successfully synthesized by surface polymerization. The particles were deposited onto a magnetic carbon paste electrode (MGCE) surface, and characterized by electrochemical measurements. Differential Pulse Voltammetry (DPV) was utilized to record the oxidative current signal that is characteristic of AHL. The detection limit of this assay was determined to be 8×10(-10)molL(-1) with a linear detection range of 2.5×10(-9)molL(-1) to 1.0×10(-7)molL(-1). This Fe3O4@SiO2-MIP-based electrochemical sensor is a valuable new tool that allows quantitative measurement of Gram-negative bacterial quorum signaling molecules. It has potential applications in the fields of clinical diagnosis or food analysis with real-time detection capability, high specificity, excellent reproducibility, and good stability.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Hui
H
Jiang
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225127, PR China.
Donglei
D
Jiang
Zhangjiagang Entry-Exit Inspection And Quarantine Bureau, Zhangjiagang, Jiangsu 215600, PR China.
Jingdong
J
Shao
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 214122, PR China. Electronic address: sxlzzz@jiangnan.edu.cn.
Xiulan
X
Sun
D054742:Acyl-Butyrolactones / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D006090:Gram-Negative Bacteria / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry; D054802:Molecular Imprinting; D053038:Quorum Sensing; D012822:Silicon Dioxide / Q000737:chemistry
D054742:Acyl-Butyrolactones; D058185:Magnetite Nanoparticles; D002244:Carbon; D012822:Silicon Dioxide
Electrochemical sensor; Fe(3)O(4); Magnetic molecularly imprinted polymers; N-acyl-l-homoserine lactones