The structure-forming role of magnesium pyrophosphate in the formation of DNA-containing microparticles during PCR.
This work is devoted to studying the mechanisms of formation of DNA-containing microparticles (MPs) during PCR. It was found that pyrophosphate, a byproduct of DNA synthesis, and magnesium cations are required for their formation, as evidenced by the results of biochemical and electron microscopy studies.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia, dan@mx.ibch.ru.
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Danilevich
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Machulin
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Sorokin
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Mulyukin
D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011756:Diphosphates / Q000378:metabolism* / Q000494:pharmacology; D015636:Magnesium Chloride / Q000494:pharmacology; D017616:Magnesium Compounds / Q000378:metabolism* / Q000494:pharmacology; D008863:Microspheres; D016133:Polymerase Chain Reaction
D011756:Diphosphates; D017616:Magnesium Compounds; D015636:Magnesium Chloride; D004247:DNA; C034732:magnesium pyrophosphate