Crystallization and crystallographic studies of kallistatin.
Kallistatin is a serine protease inhibitor (serpin) which specifically inhibits human tissue kallikrein; however, its inhibitory activity is inhibited by heparin. In order to elucidate the underlying mechanism, recombinant human kallistatin was prepared in Escherichia coli and the protein was crystallized by the sitting-drop vapour-diffusion method. X-ray diffraction data were collected to 1.9 Å resolution. The crystals were found to belong to space group P61, with unit-cell parameters a = 113.51, b = 113.51, c = 76.17 Å. Initial analysis indicated that the crystallized kallistatin was in a relaxed conformation, with its reactive-centre loop inserted in the central β-sheet.
Hongqiao International Institute of Medicine, Shanghai Tongren Hospital and Faculty of Basic Medicine, and Department of Pathophysiology, Shanghai Jiaotong University School of Medicine, (Room 1006, Building 2, No 280, South Chongqing Road), Shanghai 200025, People's Republic of China.
Fang
F
Lin
Hongqiao International Institute of Medicine, Shanghai Tongren Hospital and Faculty of Basic Medicine, and Department of Pathophysiology, Shanghai Jiaotong University School of Medicine, (Room 1006, Building 2, No 280, South Chongqing Road), Shanghai 200025, People's Republic of China.
Aiwu
A
Zhou
Hongqiao International Institute of Medicine, Shanghai Tongren Hospital and Faculty of Basic Medicine, and Department of Pathophysiology, Shanghai Jiaotong University School of Medicine, (Room 1006, Building 2, No 280, South Chongqing Road), Shanghai 200025, People's Republic of China.
Zhenquan
Z
Wei
D002852:Chromatography, Ion Exchange; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D015843:Serpins / Q000737:chemistry* / Q000302:isolation & purification; D055672:Static Electricity
D011994:Recombinant Proteins; D015843:Serpins; C051324:kallistatin
crystallization; heparin; kallistatin; serpins; tissue kallikrein