Sodium borohydride removes aldehyde inhibitors for enhancing biohydrogen fermentation.
To enhance biohydrogen production from glucose and xylose in the presence of aldehyde inhibitors, reducing agent (i.e., sodium borohydride) was in situ added for effective detoxification. The detoxification efficiencies of furfural (96.7%) and 5-hydroxymethylfurfural (5-HMF, 91.7%) with 30mM NaBH4 were much higher than those of vanillin (77.3%) and syringaldehyde (69.3%). Biohydrogen fermentation was completely inhibited without detoxification, probably because of the consumption of nicotinamide adenine dinucleotide (NADH) by inhibitors reduction (R-CHO+2NADH→R-CH2OH+2NAD(+)). Addition of 30mM NaBH4 provided the reducing power necessary for inhibitors reduction (4R-CHO+NaBH4+2H2O→4R-CH2OH+NaBO2). The recovered reducing power in fermentation resulted in 99.3% recovery of the hydrogen yield and 64.6% recovery of peak production rate. Metabolite production and carbon conversion after detoxification significantly increased to 63.7mM and 81.9%, respectively.
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Richen
R
Lin
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China. Electronic address: juncheng@zju.edu.cn.
Jun
J
Cheng
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Lingkan
L
Ding
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China; Department of Life Science and Engineering, Jining University, Jining 273155, China.
Wenlu
W
Song
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Junhu
J
Zhou
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Kefa
K
Cen
D001547:Benzaldehydes / Q000737:chemistry / Q000494:pharmacology; D056804:Biofuels; D001894:Borohydrides / Q000737:chemistry*; D005285:Fermentation / Q000187:drug effects; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*
D001547:Benzaldehydes; D056804:Biofuels; D001894:Borohydrides; D009243:NAD; C069665:syringaldehyde; D006859:Hydrogen; C025364:sodium borohydride; D014994:Xylose; C100058:vanillin; D005662:Furaldehyde; D005947:Glucose
Aldehydes; Detoxification; Hydrogen fermentation; Sodium borohydride