Gelatin and galactomannan-based scaffolds: Characterization and potential for tissue engineering applications.
In this work, we produced gelatin films containing different concentrations of galactomannan by casting solutions. The films were crosslinked by immersion in 30mM solution of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC). The crosslinking of gelatin-containing films was confirmed by the reduction of free amine band intensity (3400-3200cm(-1)) in the GEL IR, as well as by the evaluation of its behavior when immersed in phosphate-buffer solution. The crosslinking of galactomannan film was confirmed by the formation of new ether bonds, as observed by increasing intensity of the band at 1148cm(-1), and the reduction of OH band intensity (3600-3200cm(-1)). The presence of galactomannan and the crosslinking mediated by EDC were responsible to improve elasticity in the gelatin-based films. The samples did not show cytotoxicity during 24h or 48h. In addition, rat mesenchymal stem cells adhered to the films regardless of galactomannan concentration. The results indicated that the gelatin/galactomannan films are potential biomaterials for use as scaffolds for tissue engineering.
Post-Graduate Program of Materials Science, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Sala A216, CEP: 91540-000, Porto Alegre, RS, Brazil.
Nataly M
NM
Siqueira
Laboratory of Stem-Cells and Tissue Engineering, Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil.
Bruno
B
Paiva
Laboratory of Stem-Cells and Tissue Engineering, Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil.
Melissa
M
Camassola
Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH, USA.
Emily Q
EQ
Rosenthal-Kim
Institute of Chemistry, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, IQ, CEP: 91501-970, Porto Alegre, RS, Brazil.
Ketlin C
KC
Garcia
Institute of Chemistry, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, IQ, CEP: 91501-970, Porto Alegre, RS, Brazil.
Francisco P
FP
dos Santos
Post-Graduate Program of Materials Science, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Sala A216, CEP: 91540-000, Porto Alegre, RS, Brazil; Institute of Chemistry, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, IQ, CEP: 91501-970, Porto Alegre, RS, Brazil. Electronic address: soaresr@ufrgs.br.
Rosane M D
RM
Soares
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002234:Carbodiimides / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D005690:Galactose / Q000031:analogs & derivatives; D005780:Gelatin / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry*; D055595:Mechanical Phenomena; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D051379:Mice; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
C518491:1-ethyl-3-(3-(diethylamino)propyl)carbodiimide; D001672:Biocompatible Materials; D002234:Carbodiimides; D008351:Mannans; C012990:galactomannan; D005780:Gelatin; D005690:Galactose
Cell adhesion; Cytotoxicity; Galactomannan; Gelatin; Tissue engineering