Variants of human papillomavirus type 16 predispose toward persistent infection.
A cohort study of 292 Chinese women was conducted to determine the relationship between human papillomavirus (HPV) type 16 variants and persistent viral infection. Enrolled patients were HPV16 positive and had both normal cytology and histology. Flow-through hybridization and gene chip technology was used to identify the HPV type. A PCR sequencing assay was performed to find HPV16 E2, E6 and E7 gene variants. The associations between these variants and HPV16 persistent infection was analyzed by Fisher's exact test. It was found that the variants T178G, T350G and A442C in the E6 gene, as well as C3158A and G3248A variants in the E2 gene were associated with persistent HPV16 infection. No link was observed between E7 variants and persistent viral infection. Our findings suggest that detection of specific HPV variants would help identify patients who are at high risk for viral persistence and development of cervical neoplasia.
Department of Obstetrics and Gynecology, The People's Hospital of Shanghai Pudong District Shanghai, China.
Lei
L
Zhang
Clinical and Translational Research Center, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai, China ; Department of Pathology, University of Arizona College of Medicine Tucson, AZ, USA.
Hong
H
Liao
Department of Obstetrics and Gynecology, The People's Hospital of Shanghai Pudong District Shanghai, China ; Department of Pathology, University of Arizona College of Medicine Tucson, AZ, USA ; Department of Obstetrics and Gynecology, University of Arizona Tucson, AZ, USA.
Binlie
B
Yang
Department of Pathology, University of Arizona College of Medicine Tucson, AZ, USA.
Christopher P
CP
Geffre
Department of Obstetrics and Gynecology, The People's Hospital of Shanghai Pudong District Shanghai, China.
Ai
A
Zhang
Department of Obstetrics and Gynecology, The People's Hospital of Shanghai Pudong District Shanghai, China.
Aizhi
A
Zhou
Department of Obstetrics and Gynecology, The People's Hospital of Shanghai Pudong District Shanghai, China.
Huimin
H
Cao
Department of Obstetrics and Gynecology, The People's Hospital of Shanghai Pudong District Shanghai, China.
Jieru
J
Wang
Department of Pathology, University of Arizona College of Medicine Tucson, AZ, USA ; Department of Obstetrics and Gynecology, Shanghai First People's Hospital, Shanghai Jiaotong University Shanghai, China.
Zhenbo
Z
Zhang
Department of Pathology, University of Arizona College of Medicine Tucson, AZ, USA ; Department of Obstetrics and Gynecology, University of Arizona Tucson, AZ, USA ; Department of Obstetrics and Gynecology, University of Arizona Tucson, AZ, USA ; Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University Shandong, China ; Arizona Cancer Center, University of Arizona Tucson, AZ, USA.
Wenxin
W
Zheng
D000328:Adult; D000368:Aged; D002681:China; D004279:DNA, Viral / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D061809:Human Papillomavirus DNA Tests; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D009856:Oncogene Proteins, Viral / Q000235:genetics; D050725:Papillomavirus E7 Proteins / Q000235:genetics; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000821:virology*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C054855:E2 protein, Human papillomavirus type 16; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16
Human papillomavirus; persistent infection; variants