Cryoplasty for Canine Iliac Artery Stenosis and its Impact on Expression of TIMP-2 and MMP-2.
OBJECTIVE
This study was performed to observe the effects of cryoplasty on canine iliac artery stenosis and the expression of tissue inhibition of matrix metalloproteinase 2 (TIMP-2) and matrix metalloproteinase 2 (MMP-2).


METHODS
We produced a reliable canine model to mimic the atherosclerotic stenosis in the iliac artery by suturing the artery followed by vessel ligation to create an injury to intimal and medial walls. Sixteen mongrel dogs with iliac artery stenosis were randomized to conventional balloon angioplasty (n = 8) or cryoplasty (n = 8).


RESULTS
Four weeks posttreatment, the cryoplasty group with less collagen fibers and smooth muscle demonstrated significantly larger luminal diameter of iliac artery compared to the balloon angioplasty group (P < .001). Expression of TIMP-2 significantly increased and expression of MMP-2 significantly reduced in iliac artery of the cryoplasty group compared to conventional balloon angioplasty.


CONCLUSIONS
Our study suggests cryoplasty might increase the expression of TIMP-2 and decrease the expression of MMP-2, thereby inhibiting vascular hyperplasia and collagen fibers synthesis of the stenotic vessels.
Department of Interventional Radiology, Fujian Medical University Union Hospital, Fuzhou, China.
Zhengzhong
Z
Wu
Department of Pathology and Institute of Oncology, Fujian Medical University, Fuzhou, China.
Shengbing
S
Zang
Department of Pathology and Institute of Oncology, Fujian Medical University, Fuzhou, China.
Wenwen
W
Liu
Department of Interventional Radiology, Fujian Medical University Union Hospital, Fuzhou, China.
Na
N
Jiang
Department of Interventional Radiology, Fujian Medical University Union Hospital, Fuzhou, China drweizhuyang@163.com.
Weizhu
W
Yang
D000800:Angioplasty, Balloon / Q000379:methods*; D000818:Animals; D003094:Collagen / Q000378:metabolism; D003251:Constriction, Pathologic; D003452:Cryosurgery / Q000379:methods*; D004195:Disease Models, Animal; D004285:Dogs; D006965:Hyperplasia; D007083:Iliac Artery / Q000000981:diagnostic imaging / Q000201:enzymology / Q000601:surgery*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D058729:Peripheral Arterial Disease / Q000000981:diagnostic imaging / Q000201:enzymology / Q000601:surgery*; D011859:Radiography; D013997:Time Factors; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism*
D019716:Tissue Inhibitor of Metalloproteinase-2; D003094:Collagen; D020778:Matrix Metalloproteinase 2
balloon angioplasty; cryoplasty; peripheral artery disease; restenosis