Evaluation of matricellular proteins in systemic and local immune response to Mycobacterium tuberculosis infection.
Matricellular proteins such as osteopontin (OPN), galectin-9 (Gal-9), and tenascin-C (TN-C) are expressed not only under normal physiological conditions, but also during infection, inflammation and tumorigenesis. Plasma concentrations of matricellular proteins were studied to determine their diagnostic value as potential markers of tuberculosis (TB) activity. It was found that concentrations of OPN and TN-C were higher in patients with active TB than in healthy controls and individuals with latent infection. Moreover, LTBI patients had higher concentrations of OPN than did healthy controls. Gal-9 concentrations did not differ significantly between groups. Concentrations of matricellular proteins were higher in pleural fluid than in the plasma of patients with TB. Expression of matricellular proteins was also investigated in TB granulomas and other granulomatous diseases. Positive OPN and Gal-9 staining was observed in TB and sarcoidosis granulomas, but not in Crohn disease granulomas. The fibrotic ring around granulomas stained positive for TN-C in TB and sarcoidosis, but not in Crohn disease. Of the three matricellular proteins studied, OPN and TN-C may serve as reliable plasma markers for monitoring TB activity, whereas Gal-9 seems to be expressed more at the site of infection than in the systemic circulation.
Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University.|Public Health Science Program.
Fakhrial Mirwan
FM
Hasibuan
Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University.|Division of Disaster-related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 2-1 Seiryo-machi.
Beata
B
Shiratori
Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University.|Public Health Science Program.
Muhammad Andrian
MA
Senoputra
Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University.|Division of Disaster-related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 2-1 Seiryo-machi.
Haorile
H
Chagan-Yasutan
TB-HIV Research Center, Faculty of Medicine, Padjadjaran University, Jl Eicjkman 38, Bandung, 40161, West Java, Indonesia.
Raspati Cundarani
RC
Koesoemadinata
TB-HIV Research Center, Faculty of Medicine, Padjadjaran University, Jl Eicjkman 38, Bandung, 40161, West Java, Indonesia.
Lika
L
Apriani
Department of Pathology, Tohoku University Hospital, 1-1 Seiryo-machi, Aoba-ku, Sendai, 980-8574, Miyagi.
Yayoi
Y
Takahashi
Research Division, GalPharma Company, NEXT-Kagawa 204, 2217-44 Hayashi-cho, Takamatsu-shi, Kagawa, 760-0301, Japan.
Toshiro
T
Niki
TB-HIV Research Center, Faculty of Medicine, Padjadjaran University, Jl Eicjkman 38, Bandung, 40161, West Java, Indonesia.
Bachti
B
Alisjahbana
Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University.|Public Health Science Program.
Toshio
T
Hattori
D015415:Biomarkers / Q000097:blood; D003424:Crohn Disease / Q000378:metabolism; D016207:Cytokines / Q000097:blood; D037161:Galectins / Q000096:biosynthesis / Q000097:blood*; D006099:Granuloma / Q000378:metabolism; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D053495:Osteopontin / Q000096:biosynthesis / Q000097:blood*; D010994:Pleura / Q000378:metabolism; D010996:Pleural Effusion / Q000378:metabolism; D019063:Tenascin / Q000096:biosynthesis / Q000097:blood*; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000276:immunology / Q000382:microbiology
D015415:Biomarkers; D016207:Cytokines; D037161:Galectins; D019063:Tenascin; D053495:Osteopontin
galectin-9; osteopontin; tenascin-C; tuberculosis