Trichloroethylene and Its Oxidative Metabolites Enhance the Activated State and Th1 Cytokine Gene Expression in Jurkat Cells.
Trichloroethylene (TCE) is an occupational and ubiquitous environmental contaminant, and TCE exposure will increase the risk of autoimmune diseases and allergic diseases. T cells play an important role in the pathogenesis of TCE-related immune disorders, but the effect of TCE and its oxidative metabolites, trichloroacetic acid (TCA) and dichloroacetic acid (DCA), on the activation of human T cells is still unknown. In this study, Jurkat cells were pre-treated with TCE, TCA and DCA overnight and then stimulated with phorbol 12-myristate 13-acetate and ionomycin for another 4, 8 and 24 hours. IL-2 secretion was detected by ELISA; the expressions of CD25 and CD69 were tested by flow cytometry; and IFN-γ and IL-2 mRNA expression levels were investigated by real-time PCR. The results showed that TCE and its oxidative metabolites, TCA and DCA, significantly enhanced IL-2 releasing and the expression of T cell activation markers, CD25 and CD69. Consistent with this result, these compounds markedly up-regulated the expression levels of IFN-γ and IL-2 mRNA. Collectively, these findings suggest that TCE and its metabolites, TCA and DCA, might enhance the activation of T cells and disrupt various activities of peripheral T cells.
Department of Toxicology, School of Public Health, Peking University, Beijing 100191, China. yaopan408@gmail.com.|Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, China. yaopan408@gmail.com.
Yao
Y
Pan
Department of Toxicology, School of Public Health, Peking University, Beijing 100191, China. weixt@263.net.|Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, China. weixt@263.net.
Xuetao
X
Wei
Department of Toxicology, School of Public Health, Peking University, Beijing 100191, China. whao@bjmu.edu.cn.|Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, China. whao@bjmu.edu.cn.
Weidong
W
Hao
D002454:Cell Differentiation / Q000187:drug effects; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D003999:Dichloroacetic Acid / Q000633:toxicity; D004785:Environmental Pollutants / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D019169:Jurkat Cells; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018417:Th1 Cells / Q000187:drug effects* / Q000378:metabolism; D014238:Trichloroacetic Acid / Q000633:toxicity; D014241:Trichloroethylene / Q000633:toxicity*
D016207:Cytokines; D004785:Environmental Pollutants; D012333:RNA, Messenger; D014241:Trichloroethylene; D014238:Trichloroacetic Acid; D003999:Dichloroacetic Acid
IL-2; Jurkat cells; dichloroacetic acid; trichloroacetic acid; trichloroethylene