Interactions between chensinin-1, a natural antimicrobial peptide derived from Rana chensinensis, and lipopolysaccharide.
Lipopolysaccharide (LPS) plays a critical role in the pathogenesis of sepsis caused by gram-negative bacterial infections. Therefore, LPS-neutralizing molecules would have important clinical applications. Chensinin-1, a novel antimicrobial peptide with atypical structural features, was found in the skin secretions of the Chinese brown frog Rana chensinensis. To understand the role of LPS in the bacterial susceptibility to chensinin-1 and to investigate its anti-endotoxin effects, the interactions of chensinin-1 with LPS were investigated in this study using circular dichroism, in situ IR, isothermal titration calorimetry, and zeta potential. This study is the first to use in situ IR spectroscopy to evaluate the secondary structural changes of this peptide. The capacity of chensinin-1 to block the LPS-dependent cytokine secretion of macrophages was also investigated. Our results show that chensinin-1 can form α-helical structures in LPS suspensions. LPS can affect the antimicrobial activity of chensinin-1, and chensinin-1 was able to mitigate the effects of LPS. These data may facilitate the development of antimicrobial peptides with potent antimicrobial and anti-endotoxin activities.
School of Life Science, Liaoning Normal University, Dalian, 116081, China.|Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Liaoning Normal University, Dalian, 116081, China.|State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116024, China.
Weibing
W
Dong
School of Life Science, Liaoning Normal University, Dalian, 116081, China.
Yue
Y
Sun
School of Life Science, Liaoning Normal University, Dalian, 116081, China.|Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Liaoning Normal University, Dalian, 116081, China.
Dejing
D
Shang
D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry*; D023181:Antimicrobial Cationic Peptides; D002478:Cells, Cultured; D002942:Circular Dichroism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D010455:Peptides / Q000737:chemistry* / Q000187:drug effects; D000067996:RAW 264.7 Cells; D011898:Ranidae
D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010455:Peptides; C000600263:chensinin-1b
anti-endotoxic; antimicrobial peptide; in situ IR; lipopolysaccharide