A new temperature-insensitive allele of the Arabidopsis AXR6/CUL1 locus derived from a missense mutation in the C-terminal RBX1 binding region.
We isolated a new recessive allele at the AUXIN RESISTANT6/CULLIN1 (AXR6/CUL1) locus, axr6-101, from an EMS-mutagenized population of Arabidopsis thaliana, the Landsberg erecta ecotype. axr6-101 is auxin resistant and semi-dwarf similar to the other recessive axr6 mutants. The axr6-101 phenotype is caused by the E716K substitution of the CUL1 protein, which is likely to affect its ability to bind to the C-terminal RING domain of RING-box 1 (RBX1). The previously reported allele of AXR6, cul1-7, is caused by a substitution at T510 that binds to the N-terminal β-strand of RBX1. Although cul1-7 shows temperature-sensitive phenotype, the axr6-101 phenotype is largely unaffected by temperature. axr6-101 may provide an important genetic resource for study of the structure-function relationship of the CUL1 protein.
a Division of Biological Sciences; Graduate School of Environmental Earth Science; Hokkaido University ; Sapporo , Japan.
Yukiko
Y
Mori
b Department of Bioscience ; Nagahama Institute of Bio-Science and Technology ; Nagahama , Japan.
Makoto
M
Hayashi
c National Institute for Basic Biology ; Okazaki , Japan.
Mikio
M
Nishimura
a Division of Biological Sciences; Graduate School of Environmental Earth Science; Hokkaido University ; Sapporo , Japan.|d Division of Biological Sciences; Faculty of Science; Hokkaido University ; Sapporo , Japan.
Kotaro T
KT
Yamamoto
D000483:Alleles; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001665:Binding Sites; D002352:Carrier Proteins / Q000378:metabolism*; D044842:Cullin Proteins / Q000235:genetics*; D056426:Genetic Loci; D007210:Indoleacetic Acids / Q000494:pharmacology; D020125:Mutation, Missense / Q000235:genetics*; D010641:Phenotype; D011485:Protein Binding / Q000187:drug effects; D013696:Temperature
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; C100318:Cullin 1; D044842:Cullin Proteins; D007210:Indoleacetic Acids; C507151:RBX1 protein, Arabidopsis
Arabidopsis thaliana; auxin resistance; auxin signaling; axr6; cullin; mutant