Apple scar skin viroid naked RNA is actively transmitted by the whitefly Trialeurodes vaporariorum.
Nucleic acid transfer between plants is a phenomenon which is likely to occur in many ways in nature. We report here the active transmission of Apple scar skin viroid (ASSVd) naked ssRNA species by the whitefly Trialeurodes vaporariorum (Tv). Not only the viroid RNA, its DNA form was also identified from the insect. The viroid transfer efficiency was enhanced with the help of Cucumis sativus Phloem protein 2 (CsPP2), a plant protein known to translocate viroid RNAs. This PP2/ASSVd complex is stably present in the viroid infected cucumber plants, as was identified with the help of immunological reaction. As viroid-like secondary structures are found in some plant RNAs, and PP2 is known to bind and translocate several RNAs, the results have huge implications in transfer of these RNA species between plants visited by the whitefly.
a Plant Virus Lab; CSIR-Institute of Himalayan Bioresource Technology ; Palampur , India.
Yashika
Y
Walia
a Plant Virus Lab; CSIR-Institute of Himalayan Bioresource Technology ; Palampur , India.
Sunny
S
Dhir
a Plant Virus Lab; CSIR-Institute of Himalayan Bioresource Technology ; Palampur , India.
Aijaz Asghar
AA
Zaidi
a Plant Virus Lab; CSIR-Institute of Himalayan Bioresource Technology ; Palampur , India.
Vipin
V
Hallan
D000818:Animals; D018553:Cucumis sativus / Q000235:genetics* / Q000821:virology; D004277:DNA, Single-Stranded / Q000235:genetics; D022761:Gene Transfer, Horizontal; D006430:Hemiptera; D009696:Nucleic Acids / Q000235:genetics*; D037121:Plant Lectins / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D014772:Viroids / Q000235:genetics*
D004277:DNA, Single-Stranded; D009696:Nucleic Acids; D037121:Plant Lectins; D018749:RNA, Plant; C091028:phloem lectin
ASSVd DNA; Pospiviroidae; RNA transcripts; phloem protein II; viroid vector