Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes.
Recent advances in reprogramming allow us to turn somatic cells into human induced pluripotent stem cells (hiPSCs). Disease modeling using patient-specific hiPSCs allows the study of the underlying mechanism for pathogenesis, also providing a platform for the development of in vitro drug screening and gene therapy to improve treatment options. The promising potential of hiPSCs for regenerative medicine is also evident from the increasing number of publications (>7000) on iPSCs in recent years. Various cell types from distinct lineages have been successfully used for hiPSC generation, including skin fibroblasts, hematopoietic cells and epidermal keratinocytes. While skin biopsies and blood collection are routinely performed in many labs as a source of somatic cells for the generation of hiPSCs, the collection and subsequent derivation of hair keratinocytes are less commonly used. Hair-derived keratinocytes represent a non-invasive approach to obtain cell samples from patients. Here we outline a simple non-invasive method for the derivation of keratinocytes from plucked hair. We also provide instructions for maintenance of keratinocytes and subsequent reprogramming to generate integration-free hiPSC using episomal vectors.
Centre for Eye Research Australia & Department of Ophthalmology, University of Melbourne.
Sandy S C
SS
Hung
Centre for Eye Research Australia & Department of Ophthalmology, University of Melbourne.
Alice
A
Pébay
Centre for Eye Research Australia & Department of Ophthalmology, University of Melbourne; ching.wong@unimelb.edu.au.
Raymond C B
RC
Wong
D065150:Cellular Reprogramming / Q000502:physiology; D003584:Cytological Techniques / Q000379:methods*; D006197:Hair / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D015603:Keratinocytes / Q000166:cytology*