Synthesis, cytotoxicity assessment, and interaction and docking of novel palladium(II) complexes of imidazole derivatives with human serum albumin.
Imidazole analogs are the agents that attract both bioinorganic chemist and drug designer. Numerous methods have been proposed for synthesis of imidazole derivatives. In this study, a series of heterocyclic system with p-conjugated system such as 2-aryl-imidazo[4,5-f][1,10]phenanthroline analogs were synthesized. Then, three new palladium(II) complexes containing 2-(Furan-2-yl)-1H-Imidazo[4,5-f][1,10]Phenanthroline (FIP) and 2-(thiophen-2-yl)-1H-imidazo[4,5-f][1,10]phenanthroline (TIP) ligands were synthesized. The structures of the compounds, [Pd(Phen)(TIP)](NO3)2, [Pd(Phen)(FIP)](NO3)2, and [Pd(FIP)2]Cl were determined by spectroscopic methods and elemental analysis. Biological activity of the complexes synthesized was assessed against chronic myelogenous leukemia cell line, K562. Also, the interactions of human serum albumin with complexes were investigated using isothermal titration in the Tris buffer, pH 7.4. According to the results obtained, it was found that there is a set of six binding sites for these complexes on HSA with positive cooperativity in the binding process. Docking technique was also applied to confirm the experimental results. The results showed that smaller complexes have higher interaction affinity.
a Chemistry & Chemical Engineering Research Center of Iran , Tehran , Iran.
Mahboube
M
Eslami Moghadam
b Faculty of Biological Sciences, Department of Cell & Molecular Biology , Kharazmi University , Tehran , Iran.
Adeleh
A
Divsalar
c Faculty of Sciences, Department of Chemistry , Tarbiat Modares University , Tehran 14115-175 , Iran.
Abdolghafar
A
Abolhosseini Shahrnoy
d Institute of Biochemistry and Biophysics, University of Tehran , Tehran , Iran.
Ali Akbar
AA
Saboury
D001665:Binding Sites; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D007093:Imidazoles / Q000737:chemistry* / Q000633:toxicity; D008024:Ligands; D008968:Molecular Conformation; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D010165:Palladium / Q000737:chemistry*; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*; D013057:Spectrum Analysis; D013816:Thermodynamics
D007093:Imidazoles; D008024:Ligands; D012709:Serum Albumin; D010165:Palladium; C029899:imidazole
HSA binding; Pd(II) complex; cytotoxicity; docking; imidazole derivative