Isolation and characterization of dextran produced by Leuconostoc citreum NM105 from manchurian sauerkraut.
A water-soluble exopolysaccharide (EPS) was produced by Leuconostoc ctireum NM105 from homemade manchurian sauerkraut. After culturing the strain in Man-Rogosa-Sharpe medium containing 5% sucrose at 25°C for 48h, the EPS was purified and a yield of 23.5g/L was achieved. The EPS consisted exclusively of glucose and the weight-average molecular weight was 1.01×10(8)Da. The structural characterization of the purified EPS determined by FT-IR, (1)H, (13)C and two-dimensional NMR spectroscopy demonstrated that the glucan contained α-(1→6)-linked d-glucopyranose units, 2,6-linked d-glucopyranose units and terminal α-d-glucopyranose units at a ratio of 1:1:1. The microstructure of the dried dextran appeared a sheet-like smooth glittering and highly branched surface. The NM105 dextran showed high water solubility and excellent water retention. All the results suggested that the highly α-(1→2) branched dextran might have the potential to serve as valuable polymers applied in foods, cosmetics and other fields.
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
Yanping
Y
Yang
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
Qian
Q
Peng
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
Yanyun
Y
Guo
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
Ye
Y
Han
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
Huazhi
H
Xiao
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China. Electronic address: zzj@tju.edu.cn.
Zhijiang
Z
Zhou
D001937:Brassica / Q000382:microbiology*; D005285:Fermentation; D005511:Food Handling; D007934:Leuconostoc / Q000378:metabolism* / Q000502:physiology; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D012995:Solubility; D014867:Water / Q000737:chemistry
D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D014867:Water
Deuterium oxide (PubChem CID: 24602); Dextran; Ethanol (PubChem CID: 702); Exopolysaccharide; Glucose (PubChem CID: 79025); Leuconostoc citreum; Potassium bromide (PubChem CID: 253877); Potassium carbonate (PubChem CID: 11430); Structural characterization; Sucrose (PubChem CID: 5988); Trichloroacetic acid (PubChem CID: 6421); Trifluoroacetic acid (PubChem CID: 6422)