Urinary 5-hydroxymethyluracil and 8-oxo-7,8-dihydroguanine as potential biomarkers in patients with colorectal cancer.
BACKGROUND
Oxidative stress linked with chronic inflammation is associated with etiology of the colorectal cancer.


OBJECTIVE
To assess the diagnostic utility of urinary excretion of oxidatively modified DNA bases/nucleoside: 8-oxo-7,8-dihydroguanine (8-oxoGua), 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 5-hydroxymethyluracil (5-hmUra).


METHODS
Seventy-two healthy controls, 15 patients with adenomas and 56 colorectal cancer patients were recruited.


RESULTS
The receiver operating characteristic (ROC) curve analysis showed that the area under the curve (AUC) for all markers tested separately was <0.7. The combination of these modifications showed better diagnostic power (AUC = 0.778 for 8-oxoGua + 8-oxodG)/5hmUra ratio).


CONCLUSIONS
Urinary DNA modifications may reflect the oxidative stress/chronic inflammation in colorectal cancer but diagnostic performance for early-detection is moderate.
a Department of Clinical Biochemistry and.
Rafal
R
Rozalski
Daniel
D
Gackowski
Agnieszka
A
Siomek-Gorecka
Marta
M
Starczak
Martyna
M
Modrzejewska
Zbigniew
Z
Banaszkiewicz
Ryszard
R
Olinski
D000080242:8-Hydroxy-2'-Deoxyguanosine / Q000031:analogs & derivatives; D000368:Aged; D014408:Biomarkers, Tumor / Q000652:urine*; D015179:Colorectal Neoplasms / Q000652:urine*; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D008297:Male; D008875:Middle Aged; D010432:Pentoxyl / Q000031:analogs & derivatives* / Q000652:urine
D014408:Biomarkers, Tumor; C008055:5-hydroxymethyluracil; D006147:Guanine; C078307:8-oxo-7,8-dihydrodeoxyguanine; D010432:Pentoxyl; D000080242:8-Hydroxy-2'-Deoxyguanosine
5-hmUra; 8-oxoGua; DNA damage; DNA repair; oxidative stress