Advanced glycation end products and soluble receptor as markers of oxidative stress in children on hemodialysis.
BACKGROUND
Advanced glycation end products (AGEs) have biological properties that may contribute to the mortality of children on hemodialysis (HD). This study examines the relationship of LMW fluorescence AGEs, oxidized LDL (ox-LDL), soluble receptor AGE (sRAGE) as markers of oxidative stress in children with end stage renal disease (ESRD) undergoing HD.


METHODS
Thirty children with ESRD undergoing HD, and 30 healthy, age- and sex-matched children were included. Serum levels of LMW fluorescence AGEs, sRAGE, oxidized LDL (ox-LDL), pre- and post-dialysis urea, high-sensitivity C-reactive protein (hs-CRP), hemoglobin (Hb) and serum albumin (ALB), were measured.


RESULTS
Abnormal serum inflammatory changes: elevated levels of LMW AGEs, sRAGE, oxLDL, CRP and urea were exhibited in HD children compared with healthy controls; more so in anemic when compared to non-anemic patients. Significant positive correlation was found between serum levels of AGEs and sRAGE.


CONCLUSIONS
The low molecular weight form of AGEs is associated with oxidative stress in children receiving chronic HD, and may be important in the mechanisms leading to atherosclerosis and inflammation in such patients. LMW AGEs levels showed a negative correlation with sRAGE and both exhibit a significant negative relation to seum urea.
a Medical Biochemistry, National Research Centre , Cairo , Egypt .
Gamila S M
GS
El-Saeed
b Department of Pediatrics , Faculty of Medicine, Cairo University , Cairo , Egypt .
Fatina
F
Fadel
c Department of Pediatrics , National Research Centre , Cairo , Egypt , and.
Manal F
MF
Elshamaa
b Department of Pediatrics , Faculty of Medicine, Cairo University , Cairo , Egypt .
Rasha E E
RE
Galal
d Department of Clinical and Chemical Pathology , National Research Centre , Cairo , Egypt.
Eman A
EA
Elghoroury
d Department of Clinical and Chemical Pathology , National Research Centre , Cairo , Egypt.
Soha A
SA
Nasr
d Department of Clinical and Chemical Pathology , National Research Centre , Cairo , Egypt.
Eman H
EH
Thabet
d Department of Clinical and Chemical Pathology , National Research Centre , Cairo , Egypt.
Safaa M
SM
Abdelrahman
D000293:Adolescent; D050197:Atherosclerosis / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism; D002648:Child; D005260:Female; D017127:Glycation End Products, Advanced / Q000097:blood*; D006801:Humans; D007249:Inflammation / Q000097:blood; D007676:Kidney Failure, Chronic / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D018384:Oxidative Stress; D000067759:Receptor for Advanced Glycation End Products / Q000097:blood; D006435:Renal Dialysis / Q000009:adverse effects*
C000593871:AGER protein, human; D015415:Biomarkers; D017127:Glycation End Products, Advanced; D008077:Lipoproteins, LDL; D000067759:Receptor for Advanced Glycation End Products; C099252:oxidized low density lipoprotein; D002097:C-Reactive Protein
Advanced glycation end products; chronic renal failure in children; hemodialysis; markers of oxidative stress; soluble receptor AGE