Glutathione S-transferase M1 and T1 gene polymorphisms in Brazilian women with endometriosis.
OBJECTIVE
The glutathione family (GST) genes appear to play a role in the genesis of endometriosis. This case-control study aimed to compare the frequencies of GSTM1 and GSTT1 polymorphisms in women with endometriosis and women without endometriosis.


METHODS
Polymerase chain reaction was performed to analyze the GSTM1 and GSTT1 genotypes among women with surgically and histologically confirmed endometriosis (case group n = 121) and in women without evidence of endometriosis confirmed by laparoscopy for investigation the infertility or for laparoscopic tubal sterilization (control group n = 97).


RESULTS
No differences in the frequencies of GSTM1 polymorphism (null genotype) were observed between the cases and controls: odds ratio (OR) = 1.13; 95 % CI 0.656-1.93 (p = 0.659). The GSTT1 polymorphism (null genotype) was more prevalent in the endometriosis group than in the control group (OR = 0.53; 95 % CI 0.94-0.29 (p = 0.039). No relationship between menstrual cycle interval and GSTM1 null genotype frequency was observed in either cases or controls (p = 0.370 and p = 0.664, respectively). In addition, no relationship between menstrual cycle interval and GSTT1 null genotype was observed in cases (p = 0.797) or controls (p = 0.052).


CONCLUSIONS
GSTM1 null genotype frequency was similar between cases and controls. The GSTT1 null genotype was more frequent in the control group.
Federal University of Mato Grosso-UFMT, Cuiabá, MT, Brazil. eloisahelenak@yahoo.com.br.|, Av. Fernando Corrêa da Costa, n° 2367-Bairro Boa Esperança, Cuiabá, MT, ZIPMAIL: 78060-900, Brazil. eloisahelenak@yahoo.com.br.
Eloísa Helena
EH
Kubiszeski
Federal University of Mato Grosso-UFMT, Cuiabá, MT, Brazil.|Department of Gynecology and Obstetrics, Medical School, Federal University of Mato Grosso-UFMT, Cuiabá, MT, Brazil.|Tropical Institute of Reproductive Medicine and Menopause, Cuiabá, MT, Brazil.
Sebastião Freitas
SF
de Medeiros
Federal University of Mato Grosso-UFMT, Cuiabá, MT, Brazil.
Joziane Agnória
JA
da Silva Seidel
Federal University of Mato Grosso-UFMT, Cuiabá, MT, Brazil.
Jacklyne Silva
JS
Barbosa
Federal University of Mato Grosso-UFMT, Cuiabá, MT, Brazil.|Department of Basic Sciences, Medical School, Federal University of Mato Grosso-UFMT, Cuiabá, MT, Brazil.
Marcial Francis
MF
Galera
Federal University of Mato Grosso-UFMT, Cuiabá, MT, Brazil.|Department of Basic Sciences, Medical School, Federal University of Mato Grosso-UFMT, Cuiabá, MT, Brazil.
Bianca Borsatto
BB
Galera
D000328:Adult; D001938:Brazil; D016022:Case-Control Studies; D004715:Endometriosis / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008597:Menstrual Cycle / Q000235:genetics; D011110:Polymorphism, Genetic
D005982:Glutathione Transferase; C413545:glutathione S-transferase T1
DNA polymorphisms; Endometriosis; Glutathione S-transferase; Molecular biology; Null genotype