Toad skin extract cinobufatini inhibits migration of human breast carcinoma MDA-MB-231 cells into a model stromal tissue.
Toad skin extract cinobufatini study has been focused on anticancer activity, especially apoptosis-inducing activity by bufosteroids. The present study examined effect of the toad skin extract on cancer cell migration into model stromal tissues. Human breast carcinoma cell line MDA-MB-231 was incubated in the presence or absence of toad skin extract on a surface of reconstituted type I collagen gel as a model stromal tissue allowing the cells to migrate into the gel. Frozen sections were microscopically observed after azan staining. Data showed a decrease of cell number in a microscopic field and shortening of cell migration into the model stromal tissue in a dose dependent manner. This suggests that toad skin extract may possess migration-preventing activity in addition to cell toxicity such as apoptosis-inducing activity. The multifaceted effects including apoptosis-inducing and cancer cell migration-preventing activities would improve usefulness of toad skin extract cinobufatini as an anticancer medicine.
Department of Applied Biochemistry, Tokai University.
Munehiro
M
Nakata
Shuya
S
Mori
Yo
Y
Kamoshida
Shota
S
Kawaguchi
Yoko
Y
Fujita-Yamaguchi
Bo
B
Gao
Wei
W
Tang
D000664:Amphibian Venoms / Q000494:pharmacology*; D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D002025:Bufonidae / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D024042:Collagen Type I / Q000494:pharmacology; D005260:Female; D005782:Gels; D006801:Humans; D012867:Skin / Q000737:chemistry*; D017154:Stromal Cells / Q000187:drug effects / Q000473:pathology
D000664:Amphibian Venoms; D024042:Collagen Type I; D005782:Gels; C545813:huachansu