Whole genome and transcriptome sequencing of matched primary and peritoneal metastatic gastric carcinoma.
Gastric cancer is one of the most aggressive cancers and is the second leading cause of cancer death worldwide. Approximately 40% of global gastric cancer cases occur in China, with peritoneal metastasis being the prevalent form of recurrence and metastasis in advanced disease. Currently, there are limited clinical approaches for predicting and treatment of peritoneal metastasis, resulting in a 6-month average survival time. By comprehensive genome analysis will uncover the pathogenesis of peritoneal metastasis. Here we describe a comprehensive whole-genome and transcriptome sequencing analysis of one advanced gastric cancer case, including non-cancerous mucosa, primary cancer and matched peritoneal metastatic cancer. The peripheral blood is used as normal control. We identified 27 mutated genes, of which 19 genes are reported in COSMIC database (ZNF208, CRNN, ATXN3, DCTN1, RP1L1, PRB4, PRB1, MUC4, HS6ST3, MUC17, JAM2, ITGAD, IREB2, IQUB, CORO1B, CCDC121, AKAP2, ACAN and ACADL), and eight genes have not previously been described in gastric cancer (CCDC178, ARMC4, TUBB6, PLIN4, PKLR, PDZD2, DMBT1and DAB1).Additionally,GPX4 and MPND in 19q13.3-13.4 region, is characterized as a novel fusion-gene. This study disclosed novel biological markers and tumorigenic pathways that would predict gastric cancer occurring peritoneal metastasis.
Shanghai Institute of Digestive Surgery and Biobank of Gastrointestinal carcinoma, Lu Wan Qu, Shanghai, China.|Collaborative Innovation Center of Systems Biomedicine, China.
J
J
Zhang
Collaborative Innovation Center of Systems Biomedicine, China.|State Key Laboratory of Medical Genomics, Changsha City, Hunan Prov, China.
J Y
JY
Huang
Shanghai Institute of Digestive Surgery and Biobank of Gastrointestinal carcinoma, Lu Wan Qu, Shanghai, China.|Collaborative Innovation Center of Systems Biomedicine, China.
Y N
YN
Chen
Department of Pathology of Shanghai Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 200025, Shanghai, China.
F
F
Yuan
Department of Radiology of Shanghai Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 200025, Shanghai, China.
H
H
Zhang
Department of Radiology of Shanghai Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 200025, Shanghai, China.
F H
FH
Yan
State Key Laboratory of Medical Genomics, Changsha City, Hunan Prov, China.
M J
MJ
Wang
Genome Center of WuXiAppTec (Shanghai) Co., Ltd. Shanghai, 200131, China.
G
G
Wang
Genome Center of WuXiAppTec (Shanghai) Co., Ltd. Shanghai, 200131, China.
M
M
Su
Genome Center of WuXiAppTec (Shanghai) Co., Ltd. Shanghai, 200131, China.
G
G
Lu
Genome Center of WuXiAppTec (Shanghai) Co., Ltd. Shanghai, 200131, China.
Y
Y
Huang
Genome Center of WuXiAppTec (Shanghai) Co., Ltd. Shanghai, 200131, China.
H
H
Dai
Shanghai Institute of Digestive Surgery and Biobank of Gastrointestinal carcinoma, Lu Wan Qu, Shanghai, China.|Collaborative Innovation Center of Systems Biomedicine, China.
J
J
Ji
Shanghai Institute of Digestive Surgery and Biobank of Gastrointestinal carcinoma, Lu Wan Qu, Shanghai, China.|Collaborative Innovation Center of Systems Biomedicine, China.
J
J
Zhang
Shanghai Institute of Digestive Surgery and Biobank of Gastrointestinal carcinoma, Lu Wan Qu, Shanghai, China.|Collaborative Innovation Center of Systems Biomedicine, China.
J N
JN
Zhang
Shanghai Institute of Digestive Surgery and Biobank of Gastrointestinal carcinoma, Lu Wan Qu, Shanghai, China.|Collaborative Innovation Center of Systems Biomedicine, China.
Y N
YN
Jiang
Collaborative Innovation Center of Systems Biomedicine, China.|State Key Laboratory of Medical Genomics, Changsha City, Hunan Prov, China.
S J
SJ
Chen
Shanghai Institute of Digestive Surgery and Biobank of Gastrointestinal carcinoma, Lu Wan Qu, Shanghai, China.|Collaborative Innovation Center of Systems Biomedicine, China.
Z G
ZG
Zhu
Shanghai Institute of Digestive Surgery and Biobank of Gastrointestinal carcinoma, Lu Wan Qu, Shanghai, China.|Collaborative Innovation Center of Systems Biomedicine, China.
Y Y
YY
Yu
D000368:Aged; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human; D006801:Humans; D009154:Mutation / Q000235:genetics; D015514:Oncogene Proteins, Fusion / Q000235:genetics / Q000378:metabolism; D010534:Peritoneal Neoplasms / Q000235:genetics* / Q000556:secondary*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA; D015398:Signal Transduction / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D059467:Transcriptome
D015514:Oncogene Proteins, Fusion