Effect of fluoxetine and resveratrol on testicular functions and oxidative stress in a rat model of chronic mild stress-induced depression.
Our objective was to investigate the effects of chronic unpredictable mild stress (CUMS) with or without selective serotonin reuptake inhibitor (fluoxetine) and anti-oxidant (resveratrol) on testicular functions and oxidative stress in rats. Fifty male rats were divided into 2 groups; control and CUMS. CUMS group was further subdivided into 4 subgroups administered water, fluoxetine, resveratrol and both. Sucrose intake, body weight gain, serum corticosterone, serotonin and testosterone levels, sperm count and motility, testicular malondialdehyde, superoxide dismutase (SOD), catalase, glutathione (GSH), and gene expression of steroidogenic acute-regulatory (StAR) protein and cytochrome P450 side chain cleavage (P450scc) enzyme were evaluated. CUMS decreased sucrose intake, weight gain, anti-oxidants (SOD, catalase, GSH), testosterone, serotonin, StAR and cytochrome P450scc gene expression, sperm count and motility and increased malondialdehyde and corticosterone. Fluoxetine increased malondialdehyde, sucrose intake, weight gain, serotonin and decreased anti-oxidants, StAR and cytochrome P450scc gene expression, sperm count and motility, testosterone, corticosterone in stressed rats. Administration of resveratrol increased anti-oxidants, sucrose intake, weight gain, serotonin, StAR and cytochrome P450scc gene expression, testosterone, sperm count and motility, and decreased malondialdehyde and corticosterone in stressed rats with or without fluoxetine. In conclusion, CUMS induces testicular dysfunctions and oxidative stress. While treatment of CUMS rats with fluoxetine decreases the depressive behavior, it causes further worsening of testicular dysfunctions and oxidative stress. Administration of resveratrol improves testicular dysfunctions and oxidative stress that are caused by CUMS and further worsened by fluoxetine treatment.
Medical Physiology Department, Faculty of Medicine, Mansoura University, Egypt. sakr_doctor@yahoo.com.|Medical Physiology Department, College of Medicine, King Khalid University, Saudi Arabia.
H F
HF
Sakr
Medical Physiology Department, Faculty of Medicine, Mansoura University, Egypt.
A M
AM
Abbas
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Mansoura University, Egypt.
A Z
AZ
Elsamanoudy
Medical Histology and Cell Biology Department, Faculty of Medicine, Mansoura University, Egypt.
F M
FM
Ghoneim
D000818:Animals; D018687:Antidepressive Agents, Second-Generation / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001835:Body Weight; D003863:Depression / Q000188:drug therapy* / Q000209:etiology / Q000523:psychology*; D005473:Fluoxetine / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*; D013315:Stress, Psychological / Q000150:complications / Q000188:drug therapy* / Q000523:psychology*; D013550:Swimming / Q000523:psychology; D013737:Testis / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology
D018687:Antidepressive Agents, Second-Generation; D000975:Antioxidants; D013267:Stilbenes; D005473:Fluoxetine; D000077185:Resveratrol