Distinct Trafficking of Cell Surface and Endosomal TIM-1 to the Immune Synapse.
The T cell costimulatory molecule TIM-1 (T cell/transmembrane, mucin and immunoglobulin domain protein 1) sorts mainly to endosomes in lymphoid cells. At difference from the cell surface protein, endosomal TIM-1 translocates to the immune synapse (IS), where it can contribute to antigen-dependent T cell costimulation. TIM-1 ligands increase the amount of cell surface protein, preventing its traffic to the IS. The bipolar sorting of TIM-1 observed during IS formation is determined by differences in its subcellular location, and probably modulates antigen-driven immune responses.
Macromolecule Structure, Centro Nacional de Biotecnología, CNB-CSIC, Madrid, 28049, Spain.|Current address: Department of Chemistry and Molecular Biology, University of Gothenburg, Göteborg, Sweden.
Meriem
M
Echbarthi
Macromolecule Structure, Centro Nacional de Biotecnología, CNB-CSIC, Madrid, 28049, Spain.
Manuela
M
Zonca
EMBL Heidelberg, Heidelberg, 69117, Germany.
Rachel
R
Mellwig
EMBL Heidelberg, Heidelberg, 69117, Germany.
Yannick
Y
Schwab
Food and Drug Administration, Center for Biologics Evaluation and Research, Silver Spring, MD, 20993, USA.
Gerardo
G
Kaplan
Department of Medicine, Stanford University, Stanford, CA, 94305, USA.
Rosemarie H
RH
DeKruyff
Department of Immunology, School of Medicine, Complutense University, 12 de Octubre Health Research Institute, Madrid, Spain.
Pedro
P
Roda-Navarro
Macromolecule Structure, Centro Nacional de Biotecnología, CNB-CSIC, Madrid, 28049, Spain.
Jose M
JM
Casasnovas
D002462:Cell Membrane / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology*; D011992:Endosomes / Q000378:metabolism*; D000072596:Hepatitis A Virus Cellular Receptor 1; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D011991:Receptors, Virus / Q000378:metabolism*; D015398:Signal Transduction / Q000276:immunology; D013569:Synapses / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology
C491143:HAVCR1 protein, human; D000072596:Hepatitis A Virus Cellular Receptor 1; D008562:Membrane Glycoproteins; D011991:Receptors, Virus
T-cell; TIM family; immune synapse; microscopy; protein sorting; structural immunology