Structure and gene cluster of the O-antigen of Escherichia coli O43.
The O-polysaccharide (O-antigen) of Escherichia coli O43 was isolated from the lipopolysaccharide and studied by chemical methods, including sugar analyses, Smith degradation, and solvolysis with anhydrous trifluoroacetic acid, along with (1)H and (13)C NMR spectroscopy. The following structure of the pentasaccharide repeating unit of the O-polysaccharide was established: [Formula: see text] Functions of genes in the O-antigen gene cluster of E. coli O43 were assigned by a comparison with sequences in the available databases and found to be in agreement with the O-polysaccharide structure.
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: perepel@ioc.ac.ru.
Andrei V
AV
Perepelov
TEDA Institute of Biological Sciences and Biotechnology, TEDA, Nankai University, 300457 Tianjin, China.
Xi
X
Guo
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Andrei V
AV
Filatov
TEDA Institute of Biological Sciences and Biotechnology, TEDA, Nankai University, 300457 Tianjin, China.
Bin
B
Liu
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Yuriy A
YA
Knirel
D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics*
D019081:O Antigens
Bacterial polysaccharide structure; Escherichia coli; Lipopolysaccharide; O-antigen; O–antigen gene cluster