Role of adiponectin in adipose tissue wound healing.
The purpose of this study was to investigate the mechanism behind adipose tissue wound healing (ATWH). The preadipocyte cell line 3T3-L1 was cultured and expression of adiponectin receptors (AdipoR1/2) was detected by immunohistochemistry and reverse transcription polymerase chain reaction. The concentration of adiponectin secreted at different cell densities was measured by enzyme-linked immunosorbent assay, while preadipocyte proliferation and migration were determined in vitro by MTT and wound closure assays. AdipoR1/2 were found to be expressed in 3T3-L1 preadipocytes. There were no statistically significant differences in the concentrations of adiponectin secreted by cell solutions of different densities (P > 0.05). In addition, adiponectin was seen to promote the growth and migration of preadipocytes. In conclusion, adiponectin may regulate ATWH by promoting preadipocyte proliferation and migration, and its systemic and/or local application is proposed as a promising therapeutic approach for the treatment of wounds incurred as a result of surgery.
Department of Respiratory Medicine, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, Shenzhen, Guangdong, China.
C E
CE
Jin
Department of Surgery and Biological Therapy, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong, China xiaoliang_b@126.com.
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L
Xiao
Department of Surgery and Biological Therapy, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong, China.
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ZH
Ge
Department of Surgery and Biological Therapy, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong, China.
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XB
Zhan
Department of Surgery and Biological Therapy, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong, China.
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HX
Zhou
D041721:3T3-L1 Cells; D052242:Adiponectin / Q000096:biosynthesis / Q000378:metabolism*; D000273:Adipose Tissue / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D054419:Receptors, Adiponectin / Q000096:biosynthesis / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology*
D052242:Adiponectin; D054419:Receptors, Adiponectin