Genetic Manipulation of Stenotrophomonas maltophilia.
Stenotrophomonas maltophilia is a Gram-negative, aerobic, motile, environmental bacterium that is emerging as an important nosocomial pathogen with high rates of attributable mortality in severely ill patients. S. maltophilia is of particular concern to patients suffering from cystic fibrosis (CF) as it has been shown to colonize airway epithelial and establish a chronic infection. Here we describe several molecular techniques for the genetic manipulation of this bacterium, including DNA extraction, RNA extraction, conjugation of plasmids from Escherichia coli and allelic exchange.
North Dakota State University, Department of Veterinary and Microbiological Sciences, Fargo, North Dakota.
Elliott
E
Welker
North Dakota State University, Department of Veterinary and Microbiological Sciences, Fargo, North Dakota.
Yayra
Y
Domfeh
North Dakota State University, Department of Veterinary and Microbiological Sciences, Fargo, North Dakota.
Deepti
D
Tyagi
North Dakota State University, Department of Veterinary and Microbiological Sciences, Fargo, North Dakota.
Sanjivni
S
Sinha
North Dakota State University, Department of Veterinary and Microbiological Sciences, Fargo, North Dakota.
Nathan
N
Fisher
D003550:Cystic Fibrosis / Q000150:complications; D004926:Escherichia coli / Q000235:genetics; D005827:Genetics, Microbial / Q000379:methods*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D020615:Stenotrophomonas maltophilia / Q000235:genetics* / Q000302:isolation & purification; D014170:Transformation, Genetic
Stenotrophomonas maltophilia; genetic manipulation; hospital acquired infection; nosocomial