Identification and genetic characterization of two porcine astroviruses from domestic piglets in China.
Porcine astrovirus are divided into five genotypes. In this study, we identified two porcine astroviruses (AstV-LL-1 and AstV-LL-2) by using sequence-independent single-primer amplification (SISPA) on faecal specimens of healthy domestic piglets younger than 15 days. The detection rate for both was 2.82% (14/497). AstV-LLs were then sequenced and characterised. Phylogenetic analysis revealed that they have the characteristics of porcine astrovirus (PastV) 2 and 5 and have some unique genetic features. Our findings show that the two astroviruses are novel lineages of PAstV2 and 5. The findings may be helpful in evaluating the ecology and evolution of astroviruses in pigs.
National Institute for Viral Disease Control and Prevention, China CDC, 100 Ying-Xin St., Xuan-Wu District, Beijing, 100052, China.
Jin-song
JS
Li
National Institute for Viral Disease Control and Prevention, China CDC, 100 Ying-Xin St., Xuan-Wu District, Beijing, 100052, China.
Mao-zhong
MZ
Li
National Institute for Viral Disease Control and Prevention, China CDC, 100 Ying-Xin St., Xuan-Wu District, Beijing, 100052, China.
Li-shu
LS
Zheng
National Institute for Viral Disease Control and Prevention, China CDC, 100 Ying-Xin St., Xuan-Wu District, Beijing, 100052, China.
Na
N
Liu
National Institute for Viral Disease Control and Prevention, China CDC, 100 Ying-Xin St., Xuan-Wu District, Beijing, 100052, China.
Dan-di
DD
Li
National Institute for Viral Disease Control and Prevention, China CDC, 100 Ying-Xin St., Xuan-Wu District, Beijing, 100052, China. zhaojund@126.com.
Zhao-jun
ZJ
Duan
D000818:Animals; D000829:Animals, Domestic / Q000821:virology; D019350:Astroviridae Infections / Q000662:veterinary* / Q000821:virology; D002681:China; D005243:Feces / Q000821:virology; D005838:Genotype; D001257:Mamastrovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
Domestic pig; Faeces; Porcine astrovirus