Identification of candidate biomarker mass (m/z) ranges in serous ovarian adenocarcinoma using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling.
OBJECTIVE
To differentiate plasma from ovarian cancer and healthy individuals using MALDI-TOF mass spectroscopy.


METHODS
MALDI-TOF was used to generate profiles of immuno-depleted plasma samples (89 cancers and 199 healthy individuals) that were fractionated using three types of magnetic beads (HIC8, WCX and IMAC-Cu).


RESULTS
Differentially expressed mass ranges showing >1.5-2-fold change in expression from HIC8 (30), WCX (12) and IMAC-Cu (6) fractions were identified. Cross validation and recognition capability scores for the models indicated discrimination between the classes.


CONCLUSIONS
Spectral profiles can differentiate plasma samples of ovarian cancer patients from healthy individuals.
a Department of Molecular Oncology .
Amutha
A
Periyasamy
Gopal
G
Gopisetty
Sridevi
S
Veluswami
Malliga
M
Joyimallaya Subramanium
Rajkumar
R
Thangarajan
D000230:Adenocarcinoma / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008970:Molecular Weight; D010051:Ovarian Neoplasms / Q000097:blood*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D014408:Biomarkers, Tumor; D001798:Blood Proteins
HIC8; IMAC-Cu; MALDI-TOF; WCX; ovarian cancer; plasma