[A gain-of-function mutation in FGFR2 influences mandibular condylar development on mice].
The development of the skeleton is regulated by numerous signaling molecules expressed in epiphyseal cartilage controlling both chondrogenesis and osteogenesis such as fibroblast growth factor receptors (FGFRs). In order to explore the important effect of fibroblast growth factor receptor 2 (FGFR2) in the process of mandibular condylar growth, we introduced gain-of-function Fgfr2(+/S252W) mice, and investigated mandibular condylar morphology by means of safranin-o/fast green staining at the stage of 1 week, 3 weeks and 6 weeks. The mutant mice displayed narrower width of the mandibular condylar growth plate, stronger stainings of trabecular bone at the stage of 1 week, 3 weeks and 6 weeks and faster degradation of the calcified cartilage cell layer at the stage of 6 weeks. We also assessed the expression of type X collagen (Col X) in mandibular condyle at the stage of 3 weeks by immunohistochemical staining and real-time PCR. The results showed that Col X was increased in the mutant mice. In conclusion, the gain-of-function mutation in FGFR2 resulted in histopathological abnormalities and development deformity of mandibular condyle cartilage in mice, which inhibited endochondral bone formation.
Department of Stomatology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Xin
X
Liang
Department 4, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China.
Bo
B
Zhang
Department 4, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China.
Ping
P
Liu
Department 4, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China.
Tu-jun
TJ
Weng
Department 4, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China.
Li
L
Zhang
Department of Stomatology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Long-zhu
LZ
He
Department 4, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China.
Fang-fei
FF
Li
Department of Stomatology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Chen
C
Qu
Department of Stomatology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Ping
P
Wang
D000818:Animals; D008335:Mandibular Condyle / Q000002:abnormalities*; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D016133:Polymerase Chain Reaction; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000235:genetics*
C496354:Fgfr2 protein, mouse; D051497:Receptor, Fibroblast Growth Factor, Type 2