Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip.
Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays. Chip-based, quantum dot (QD)-bead-biomolecule probes have been used for the detection of various types of DNA. In this study, we developed dopamine (DA)-functionalized InP/ZnS QDs (QDs-DA) as fluorescence probes for the detection of adenosine in microfluidic chips. The photoluminescence (PL) intensity of the QDs-DA is quenched by Zn(2+) because of the strong coordination interactions. In the presence of adenosine, Zn(2+) cations preferentially bind to adenosine, and the PL intensity of the QDs-DA is recovered. A polydimethylsiloxane-based microfluidic chip was fabricated, and adenosine detection was confirmed using QDs-DA probes.
Department of chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-Do, South Korea.
Seshadri Reddy
SR
Ankireddy
Department of chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-Do, South Korea.
Jongsung
J
Kim
D000241:Adenosine / Q000032:analysis*; D004298:Dopamine / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D007204:Indium / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010720:Phosphines / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
D005456:Fluorescent Dyes; D010720:Phosphines; D013440:Sulfides; D017967:Zinc Compounds; D007204:Indium; D000241:Adenosine; C031238:zinc sulfide; C090882:indium phosphide; D004298:Dopamine
PDMS; adenosine; mercaptopropionic acid; mercaptoundecanoic acid; microfluidic chip; photoluminescence; quantum dots