[SOI-nanowire biosensor for the detection of D-NFAT 1 protein].
The nanowire (NW) detection is one of fast-acting and high-sensitive methods allowing to reveal potentially relevant protein molecules. A NW biosensor based on the silicon-on-insulator (SOI)-structures was used for biospecific label-free detection of NFAT 1 (D-NFAT 1) oncomarker in real time. For this purpose, SOI-nanowires (NWs) were modified with aptamers against NFAT 1 used as molecular probes. It was shown that using this biosensor it is possible to reach the sensitivity of ~10(-15) M. This sensitivity was comparable with that of the NW biosensor with immobilized antibodies used as macromolecular probes. The results demonstrate promising approaches used to form the sensor elements for high-sensitive disease diagnostics.
Institute of Biomedical Chemistry, Moscow, Russia.
K A
KA
Malsagova
Institute of Biomedical Chemistry, Moscow, Russia.
Yu D
YD
Ivanov
Institute of Biomedical Chemistry, Moscow, Russia.
T O
TO
Pleshakova
Institute of Biomedical Chemistry, Moscow, Russia.
A F
AF
Kozlov
Institute of Biomedical Chemistry, Moscow, Russia.
N V
NV
Krohin
Institute of Biomedical Chemistry, Moscow, Russia.
A L
AL
Kaysheva
Institute of Biomedical Chemistry, Moscow, Russia.
I D
ID
Shumov
Rzhanov Institute of Semiconductor Physics, Novosibirsk, Russia.
V P
VP
Popov
Rzhanov Institute of Semiconductor Physics, Novosibirsk, Russia.
O V
OV
Naumova
Rzhanov Institute of Semiconductor Physics, Novosibirsk, Russia.
B I
BI
Fomin
Rzhanov Institute of Semiconductor Physics, Novosibirsk, Russia.
D A
DA
Nasimov
D000911:Antibodies, Monoclonal / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000138:chemical synthesis / Q000737:chemistry*; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D055664:Electrochemical Techniques; D006801:Humans; D057230:Limit of Detection; D050778:NFATC Transcription Factors / Q000032:analysis*; D053770:Nanowires / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000032:analysis*; D012822:Silicon Dioxide / Q000737:chemistry; D012996:Solutions; D013388:Succinimides / Q000737:chemistry
D000911:Antibodies, Monoclonal; D052157:Aptamers, Nucleotide; D050778:NFATC Transcription Factors; C494006:NFATC2 protein, human; D009363:Neoplasm Proteins; D012996:Solutions; D013388:Succinimides; D012822:Silicon Dioxide; C011240:dithiobis(succinimidylpropionate)
D-NFAT 1; aptamer; biosensor; nanowires; silicon-on-insulator