Preparation and bioactive properties of chitosan and casein phosphopeptides composite coatings for orthopedic implants.
Using the layer-by-layer deposition method, functional chitosan/casein phospopeptides (CS/CPP) composite coatings were produced on Co-Cr-Mo alloy. The CS/CPP composite coatings had the dendritic topography, and were quite hydrophilic. Zeta potential measurements showed the composite coatings were negative charged at neural pH. XPS results indicated that the CS/CPP composite coatings were covalently bond to the substrate. When MC3T3-E1 cells were seeded on the CS/CPP composite coatings, no cytotoxicity was observed. The bone morphogenetic protein-2 (BMP-2) mRNA expression was significantly up-regulated in MC3T3-E1 cells cultured on the composite coatings and it was twice as much as that of cells cultured on the bare substrate. The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA were increased 5-fold and 55-fold, respectively. These results suggested the CS/CPP composite coatings may have potential application in cobalt matrix orthopaedic implants.
Key Laboratory of Education Ministry for Modern Design and Rotor-Bearing System, Xi'an Jiaotong University, Xi'an 710049, PR China.
Liguo
L
Qin
Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China.
Huanhuan
H
Dong
Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China.
Ziqing
Z
Mu
Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China. Electronic address: yarlee@163.com.
Yali
Y
Zhang
Key Laboratory of Education Ministry for Modern Design and Rotor-Bearing System, Xi'an Jiaotong University, Xi'an 710049, PR China. Electronic address: donggn@mail.xjtu.edu.cn.
Guangneng
G
Dong
D016475:3T3 Cells; D000497:Alloys / Q000737:chemistry; D000818:Animals; D002364:Caseins / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D008422:Materials Testing; D051379:Mice; D009985:Orthopedics; D053244:Osteoprotegerin / Q000235:genetics; D010748:Phosphopeptides / Q000737:chemistry*; D019736:Prostheses and Implants; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013499:Surface Properties
D000497:Alloys; D002364:Caseins; D020099:Coated Materials, Biocompatible; D053244:Osteoprotegerin; D010748:Phosphopeptides; D012333:RNA, Messenger; D048271:Chitosan
Bioactivity; Casein phospopeptides; Chitosan; Co–Cr–Mo alloy; Layer-by-layer