Conjugated Polymer-Based Hybrid Nanoparticles with Two-Photon Excitation and Near-Infrared Emission Features for Fluorescence Bioimaging within the Biological Window.
Hybrid fluorescent nanoparticles (NPs) capable of fluorescing near-infrared (NIR) light (centered ∼730 nm) upon excitation of 800 nm laser light were constructed. A new type of conjugated polymer with two-photon excited fluorescence (TPEF) feature, P-F8-DPSB, was used as the NIR-light harvesting component and the energy donor while a NIR fluorescent dye, DPA-PR-PDI, was used as the energy acceptor and the NIR-light emitting component for the construction of the fluorescent NPs. The hybrid NPs possess δ value up to 2.3 × 10(6) GM per particle upon excitation of 800 nm pulse laser. The excellent two-photon absorption (TPA) property of the conjugated polymer component, together with its high fluorescence quantum yield (ϕ) up to 45% and the efficient energy transfer from the conjugated polymer to NIR-emitting fluorophore with efficiency up to 90%, imparted the hybrid NPs with TPEF-based NIR-input-NIR-output fluorescence imaging ability with penetration depth up to 1200 μm. The practicability of the hybrid NPs for fluorescence imaging in Hela cells was validated.
School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS) , Beijing 100049, China.
Yanlin
Y
Lv
Institute of Polymer Optoelectronic Materials & Devices, State Key Laboratory of Luminescent Materials & Devices, South China University of Technology , Guangzhou, 510640, China.
Peng
P
Liu
School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS) , Beijing 100049, China.
Hui
H
Ding
Beijing National Laboratory for Molecular Science (BNLMS) and Key Laboratory for Photochemistry, Institute of Chemistry Chinese Academy of Sciences , Beijing, 100190, China.
Yishi
Y
Wu
Beijing National Laboratory for Molecular Science (BNLMS) and Key Laboratory for Photochemistry, Institute of Chemistry Chinese Academy of Sciences , Beijing, 100190, China.
Yongli
Y
Yan
School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS) , Beijing 100049, China.
Heng
H
Liu
School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS) , Beijing 100049, China.
Xuefei
X
Wang
Institute of Polymer Optoelectronic Materials & Devices, State Key Laboratory of Luminescent Materials & Devices, South China University of Technology , Guangzhou, 510640, China.
Fei
F
Huang
Beijing National Laboratory for Molecular Science (BNLMS) and Key Laboratory for Photochemistry, Institute of Chemistry Chinese Academy of Sciences , Beijing, 100190, China.
Yongsheng
Y
Zhao
School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS) , Beijing 100049, China.
Zhiyuan
Z
Tian
D065638:Absorption, Radiation; D002470:Cell Survival; D003952:Diagnostic Imaging / Q000379:methods*; D000067493:Dynamic Light Scattering; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D017785:Photons; D011108:Polymers / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
D005456:Fluorescent Dyes; D011108:Polymers
conjugated polymer; energy transfer; multiphoton fluorescence bioimaging; near-infrared (NIR) fluorescence; two-photon absorption