Visualization of Actin Cytoskeletal Dynamics in Fixed and Live Drosophila Egg Chambers.
Visualization of actin cytoskeletal dynamics is critical for understanding the spatial and temporal regulation of actin remodeling. Drosophila oogenesis provides an excellent model system for visualizing the actin cytoskeleton. Here, we present methods for imaging the actin cytoskeleton in Drosophila egg chambers in both fixed samples by phalloidin staining and in live egg chambers using transgenic actin labeling tools.
Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, 51 Newton Rd, 1-550 BSB, Iowa City, IA, 52242, USA.
Christopher M
CM
Groen
Tina L
TL
Tootle
D000199:Actins / Q000378:metabolism*; D000818:Animals; D003599:Cytoskeleton / Q000235:genetics / Q000648:ultrastructure*; D004331:Drosophila melanogaster / Q000235:genetics; D005260:Female; D008853:Microscopy; D008967:Molecular Biology / Q000379:methods*; D009866:Oogenesis / Q000235:genetics*; D010063:Ovum / Q000254:growth & development / Q000378:metabolism
D000199:Actins