Rapid-response fluorescent probe for hydrogen peroxide in living cells based on increased polarity of C-B bonds.
Hydrogen peroxide (H2O2) as a reactive oxygen species (ROS) plays a crucial role in oxidative stress and signal transduction of organisms. Currently, a fluorescence probe has proven to be a powerful tool for the H2O2 analysis. However, the common problem is the slow response, causing difficulty in tracking H2O2 in situ. Herein, we describe a novel aggregation-induced emission (AIE) fluorescence probe based on increased polarity of C-B bonds that is well suited for monitoring H2O2 rapidly and selectively. Importantly, the probe was successfully applied to visualize H2O2 levels in living cells, which provides a rapid-response and highly selective fluorescence tool for monitoring of the H2O2 levels in biological process.
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University , Jinan 250014, People's Republic of China.
Wei
W
Zhang
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University , Jinan 250014, People's Republic of China.
Wei
W
Liu
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University , Jinan 250014, People's Republic of China.
Ping
P
Li
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University , Jinan 250014, People's Republic of China.
Fang
F
Huang
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University , Jinan 250014, People's Republic of China.
Hui
H
Wang
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University , Jinan 250014, People's Republic of China.
Bo
B
Tang
D000818:Animals; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D008264:Macrophages / Q000737:chemistry / Q000166:cytology; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D061848:Optical Imaging / Q000379:methods; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide