Chemerin is a novel regulator of lactogenesis in bovine mammary epithelial cells.
Chemerin is a chemoattractant cytokine (chemokine) produced by adipocytes and hepatocytes; it regulates insulin sensitivity and adipocyte differentiation. The objective of this study was to investigate the effect of chemerin on the expression of genes related to lactogenesis and the regulators of chemerin signaling in a bovine mammary epithelial cell line (MAC-T). Two types of chemerin receptors, chemokine like-receptor 1 (CMKLR1) and chemokine (C-C motif) receptor-like 2 (CCRL2), were detected in cultured MAC-T cells, whereas chemerin was not detected. G protein-coupled receptor 1 (GPR1), another receptor of chemerin, was undetectable in MAC-T cells. Chemerin upregulated transcript expression of CMKLR1, CCRL2, and genes associated with fatty acid synthesis, glucose uptake, insulin signaling, and casein synthesis in MAC-T cells. Lactogenic hormones (insulin, growth hormone, and prolactin) downregulated the expression of CMKLR1 in MAC-T cells. Adiponectin suppressed CMKLR1 expression. TNF-α suppressed CMKLR1, but induced CCRL2 expression. These data suggest chemerin is a novel regulator of lactogenesis via its own receptor in bovine mammary epithelial cells.
Lab of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan.
Yutaka
Y
Suzuki
Lab of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan; Grassland Management Research Division, NARO Institute of Livestock and Grassland Science, Nasushiobara, Tochigi, Japan.
Satoshi
S
Haga
Lab of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan.
Daiki
D
Katoh
Lab of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan.
Kyoung-ha
KH
So
Grassland and Forage Division, National Institute of Animal Science, RDA, Cheonan, South Korea.
Ki-choon
KC
Choi
Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul, South Korea.
U-suk
US
Jung
Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul, South Korea.
Hong-gu
HG
Lee
Lab of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan.
Kazuo
K
Katoh
Lab of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. Electronic address: sanggun.roh.a3@tohoku.ac.jp.
Sang-gun
SG
Roh
D017667:Adipocytes / Q000378:metabolism; D052242:Adiponectin / Q000378:metabolism; D000818:Animals; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D018925:Chemokines / Q000378:metabolism*; D002630:Chemotactic Factors / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006728:Hormones / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008892:Milk / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D052242:Adiponectin; D018925:Chemokines; D002630:Chemotactic Factors; D006728:Hormones; D019707:Receptors, Chemokine; D014409:Tumor Necrosis Factor-alpha
Bovine mammary epithelial cell; Chemerin; Hormonal regulation; Lactogenesis