Expression of the mammalian peptide hormone obestatin in Trichoderma reesei.
The filamentous fungus Trichoderma reesei is an expression host widely exploited for the production of recombinant proteins. However, its capacity for expressing small peptides (<20 kDa) has remained largely uncharted to date. In this work, we have produced the hormone peptide obestatin fused to the hydrophobin I tag (Obe-HFBI), using the T. reesei cellobiohydrolase I core (CBHI) or xylanase 2 (XYN2) pro-region as a carrier and the cbh1 promoter for gene expression, in high protein-low protease producing mutant strains T. reesei Rut-C30 and HEPI. The yield of obestatin was improved from about 300 ng/ml to up to 5.5 μg/ml through adaptive laboratory evolution and modifications to the cultivation strategy, which included adjustments of the type and ratio of carbon and nitrogen sources used in the medium. The successful expression of Obe-HFBI demonstrated the potential of T. reesei as an expression host for small peptides and further enhancement of the recombinant yield through modification of culture conditions.
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia; Biomolecular Frontiers Research Centre, Macquarie University, Sydney, Australia. Electronic address: angela.sun@mq.edu.au.
Angela
A
Sun
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia; Biomolecular Frontiers Research Centre, Macquarie University, Sydney, Australia.
Robyn
R
Peterson
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia; Biomolecular Frontiers Research Centre, Macquarie University, Sydney, Australia; School of Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, Australia.
Junior
J
Te'o
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia; Biomolecular Frontiers Research Centre, Macquarie University, Sydney, Australia.
Helena
H
Nevalainen
D000595:Amino Acid Sequence; D000818:Animals; D018533:Biomass; D018628:Gene Dosage; D015870:Gene Expression; D005822:Genetic Vectors / Q000378:metabolism; D054439:Ghrelin / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008322:Mammals / Q000378:metabolism*; D008969:Molecular Sequence Data; D055550:Protein Stability; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015203:Reproducibility of Results; D014170:Transformation, Genetic; D014242:Trichoderma / Q000254:growth & development / Q000378:metabolism*
D054439:Ghrelin; D011993:Recombinant Fusion Proteins