Epstein-Barr Viruses (EBVs) Deficient in EBV-Encoded RNAs Have Higher Levels of Latent Membrane Protein 2 RNA Expression in Lymphoblastoid Cell Lines and Efficiently Establish Persistent Infections in Humanized Mice.
Functions of Epstein-Barr virus (EBV)-encoded RNAs (EBERs) were tested in lymphoblastoid cell lines containing EBER mutants of EBV. Binding of EBER1 to ribosomal protein L22 (RPL22) was confirmed. Deletion of EBER1 or EBER2 correlated with increased levels of cytoplasmic EBV LMP2 RNA and with small effects on specific cellular microRNA (miRNA) levels, but protein levels of LMP1 and LMP2A were not affected. Wild-type EBV and EBER deletion EBV had approximately equal abilities to infect immunodeficient mice reconstituted with a human hematopoietic system.
Section of Virology, Imperial College, London, United Kingdom.
Goran
G
Gregorovic
Section of Virology, Imperial College, London, United Kingdom.
Elizabeth A
EA
Boulden
Section of Virology, Imperial College, London, United Kingdom.
Rachel
R
Bosshard
Section of Virology, Imperial College, London, United Kingdom.
Claudio
C
Elgueta Karstegl
Department of Molecular Genetics and Microbiology and Center for Virology, Duke University Medical Center, Durham, North Carolina, USA.
Rebecca
R
Skalsky
Department of Molecular Genetics and Microbiology and Center for Virology, Duke University Medical Center, Durham, North Carolina, USA.
Bryan R
BR
Cullen
Viral Immunobiology, Institute for Experimental Immunology, University of Zürich, Switzerland.
Cornelia
C
Gujer
Viral Immunobiology, Institute for Experimental Immunology, University of Zürich, Switzerland.
Patrick
P
Rämer
Viral Immunobiology, Institute for Experimental Immunology, University of Zürich, Switzerland.
Christian
C
Münz
Section of Virology, Imperial College, London, United Kingdom p.farrell@imperial.ac.uk.
Paul J
PJ
Farrell
D000818:Animals; D002460:Cell Line; D002945:Cisplatin / Q000494:pharmacology; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D016513:Mice, SCID; D035683:MicroRNAs / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; C099537:Epstein-Barr virus encoded RNA 1; C106732:Epstein-Barr virus encoded RNA 2; D035683:MicroRNAs; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; D016159:Tumor Suppressor Protein p53; D014763:Viral Matrix Proteins; C067169:RPL22 protein, human; D002945:Cisplatin