Effect of Himatanthus sucuuba in Maternal Reproductive Outcome and Fetal Anomaly Frequency in Rats.
The aim of this study was to evaluate the effect of Himatanthus sucuuba on the maternal reproductive outcome and fetal anomaly incidence in rats. Pregnant rats were randomly divided into three experimental groups as follows: Control = treated with water (vehicle), treated 250 = treated with H. sucuuba at dose 250 mg/kg, and treated 500 = treated with H. sucuuba at dose 500 mg/kg. The rats were orally treated, by gavage, with H. sucuuba or vehicle (water) during preimplantation and organogenic period (from gestational day 0-14). At day 21 of pregnancy, all rats were killed to obtain maternal-fetal data. The treatment with H. sucuuba at dose of 250 mg/kg caused reduction in placental efficiency and an increase preimplantation loss rate and placenta weight compared with the control. The treated 500 group presented a significant decrease in maternal weight gain, maternal weight gain minus gravid uterus weight, fetal weight, and placental efficiency compared with the control. In this group, there was a decrease in body weight at day 20 of pregnancy and metacarpus ossification and an increase in the preimplantation loss rate and skeletal anomalies compared with other groups. Himatanthus sucuuba extract caused intrauterine growth restriction, preimplantation loss, and developmental delay in the high doses tested.
Laboratory of System Physiology and Reproductive Toxicology, Institute of Biological and Health Sciences, Federal University of Mato Grosso (UFMT), Barra do Garças, Mato Grosso State, Brazil.
Thaigra
T
de Sousa Soares
Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, Univ Estadual Paulista_Unesp, Botucatu, São Paulo State, Brazil.
Débora Cristina
DC
Damasceno
Department of Morphology, Institute of Biosciences of Botucatu, Univ Estadual Paulista (Unesp), Botucatu, São Paulo State, Brazil.
Wilma De Grava
Wde G
Kempinas
Laboratory of System Physiology and Reproductive Toxicology, Institute of Biological and Health Sciences, Federal University of Mato Grosso (UFMT), Barra do Garças, Mato Grosso State, Brazil.
Flávia Mayara Campos
FM
Resende
Instituto Estadual de Pesquisa do Amapá (IEPA), Amapá State, Brazil.
Maria Aparecida
MA
Correa dos Santos
Department of Physiology, Institute of Biosciences of Botucatu, Univ Estadual Paulista (Unesp), Botucatu, São Paulo State, Brazil.
Clélia Akiko
CA
Hiruma-Lima
Laboratory of System Physiology and Reproductive Toxicology, Institute of Biological and Health Sciences, Federal University of Mato Grosso (UFMT), Barra do Garças, Mato Grosso State, Brazil.|Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, Univ Estadual Paulista_Unesp, Botucatu, São Paulo State, Brazil.
Gustavo Tadeu
GT
Volpato
D000818:Animals; D029062:Apocynaceae / Q000737:chemistry*; D001835:Body Weight / Q000187:drug effects; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D005333:Fetus / Q000002:abnormalities* / Q000187:drug effects; D008297:Male; D010012:Osteogenesis / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D011247:Pregnancy; D017208:Rats, Wistar; D012098:Reproduction / Q000187:drug effects*; D014867:Water
D010936:Plant Extracts; D014867:Water
Himatanthus sucuuba; malformation; pregnancy; rats; reproductive outcome