Borate-fructose complex: A novel mediator for laccase and its new function for fructose determination.
Laccase and borate-fructose complex were investigated by coincidence in a solid-state fermentation of Edenia sp. TS-76 under fructose oxidase screening. Laccase was purified to homogeneity with a 34-fold purification and 32% yield. Fructose had no significant effect on laccase activity, whereas borate reduced laccase activity by 60-90%; conversely, the borate-fructose complex increased laccase activity by nearly fourfold at pH 7.5. The complex caused a shift in the optimal pH for laccase from 5.0 to 7.5 and served as a highly efficient mediator. Borate complexed with fructose provides an alternative, time-saving, and specific method for serum fructose determination.
Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, Taiwan.
Chih-Yu
CY
Cheng
Department of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan.
Chia-I
CI
Liao
Department of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan. Electronic address: sflin@nuk.edu.tw.
Shuen-Fuh
SF
Lin
D001203:Ascomycota / Q000201:enzymology* / Q000378:metabolism; D052160:Benzothiazoles / Q000378:metabolism; D055162:Biocatalysis / Q000187:drug effects; D001881:Borates / Q000378:metabolism* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D005285:Fermentation; D005632:Fructose / Q000378:metabolism* / Q000494:pharmacology; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D042845:Laccase / Q000302:isolation & purification / Q000378:metabolism*; D008670:Metals / Q000378:metabolism / Q000494:pharmacology; D010084:Oxidation-Reduction; D013451:Sulfonic Acids / Q000378:metabolism; D013696:Temperature
D052160:Benzothiazoles; D001881:Borates; D005656:Fungal Proteins; D008670:Metals; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D005632:Fructose; D042845:Laccase
Borate–fructose complex; Fructose determination; Laccase; Mediator