Anti-proliferative and anti-invasive effects of ferulic acid in TT medullary thyroid cancer cells interacting with URG4/URGCP.
Ferulic acid (4-hydroxy-3-methoxycinnamic acid; FA), a common dietary plant phenolic compound, is abundant in fruits and vegetables. The aim of present study is to investigate the effects of FA on cell cycle, apoptosis, invasion, migration, and colony formation in the TT medullary thyroid cancer cell line. The effect of FA on cell viability was determined by using CellTiter-Glo assay. IC50 dose in the TT cells was detected as 150 μM. URG4/URGCP (upregulated gene-4/upregulator of cell proliferation) is a novel gene in full-length mRNA of 3.607 kb located on 7p13. It was determined that FA caused a decrease in the expression of novel gene URG4/URGCP, CCND1, CDK4, CDK6, BCL2, MMP2, and MMP9, a significant increase in the expression of p53, PARP, PUMA, NOXA, BAX, BID, CASP3, CASP9, and TIMP1 genes in TT human thyroid cancer cell line by using real-time PCR. It was found that FA in TT cells suppressed invasion, migration, and colony formation by using matrigel invasion chamber, wound healing, and colony formation assay, respectively. In conclusion, it is thought that FA indicates anticarcinogenesis activity by affecting cell cycle arrest, apoptosis, invasion, migration, and colony formation on TT cells.
Department of Medical Biology, Pamukkale University, School of Medicine, Denizli, Turkey. yavuzdodurga@gmail.com.
Yavuz
Y
Dodurga
Department of Medical Biology, Necmettin Erbakan University, School of Medicine, Konya, Turkey.
Canan
C
Eroğlu
Department of Medical Biology, Pamukkale University, School of Medicine, Denizli, Turkey.
Mücahit
M
Seçme
Department of Medical Biology, Pamukkale University, School of Medicine, Denizli, Turkey.
Levent
L
Elmas
Department of Medical Biology, Ege University, School of Medicine, İzmir, Turkey.
Çığır Biray
ÇB
Avcı
Department of Forensic Medicine, Ankara University, School of Medicine, Ankara, Turkey.
N Lale
NL
Şatıroğlu-Tufan
D017209:Apoptosis / Q000187:drug effects; D018278:Carcinoma, Neuroendocrine / Q000188:drug therapy* / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003373:Coumaric Acids / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013964:Thyroid Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D003373:Coumaric Acids; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C470990:URGCP protein, human; C004999:ferulic acid
Ferulic acid; TT cells; Thyroid cancer; URG4/URGCP