Antioxidant and in vitro anticancer activities of phenolics isolated from sugar beet molasses.
BACKGROUND
In the present study, the phenolic compounds were prepared using ultrasonic-aid extraction from sugar beet molasses (SBM).


METHODS
Gallic acid (GA), cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP) were produced after column chromatography from the extraction, and further detected using NMR, QTOF-MS and ESI-MS/MS.


RESULTS
The three compounds exhibited strong antioxidant activities including DPPH radical scavenging activities, ABTS radical scavenging activities and ORAC values. GA showed the strongest antioxidant activity. Antitumor activities significantly increased in a dose-dependent manner. In particular, the CGC had growth inhibitory activities of 94.86, 87.27 and 67.13% against the human colon (CACO-2), hepatocellular (HepG2) and breast (MCF-7) carcinoma cell lines, respectively, at the highest concentration of 400 μg/mL of the extracts. These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.


CONCLUSIONS
The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.
College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan road, Guangzhou, 510640, China.
Mingshun
M
Chen
College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan road, Guangzhou, 510640, China.
Hecheng
H
Meng
College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan road, Guangzhou, 510640, China.
Yi
Y
Zhao
College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan road, Guangzhou, 510640, China.
Fuquan
F
Chen
College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan road, Guangzhou, 510640, China. shujuanyu8@gmail.com.|Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou, 510640, China. shujuanyu8@gmail.com.
Shujuan
S
Yu
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D027461:Beta vulgaris / Q000737:chemistry*; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008965:Molasses; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology
D000970:Antineoplastic Agents; D000975:Antioxidants; D010936:Plant Extracts