Increasing the stearate content in seed oil of Brassica juncea by heterologous expression of MlFatB affects lipid content and germination frequency of transgenic seeds.
Fatty acids from dietary lipids can impart both beneficial and harmful health effects. The compositional balance between saturated and unsaturated fatty acids plays a decisive role in maintaining the physiological harmony, proper growth and development in the human system. In case of Brassica juncea seed oil, the level of saturated fatty acid, especially desirable stearate is very much lower than the recommended value, along with a high content of nutritionally undesirable erucic acid. Therefore, in order to shift the carbon flux towards the production of stearate at the expense of erucate, the MlFatB gene encoding a FatB thioesterase from Madhuca longifolia (latifolia) was expressed heterologously in seed tissues of B. juncea. The functional MlFatB competed with the highly active endogenous BjFatA thioesterase, and the transgenic B. juncea lines showed noteworthy changes in their seed fatty acid profiles. The proportion of stearate increased up to 16-fold, constituting almost 31% of the total fatty acids along with the production of arachidic acid in significant amount (up to ∼11%). Moreover, the content of erucate was reduced up to 71% in the seed oils of transgenic lines. Although a nutritionally desirable fatty acid profile was achieved, the transgenic seeds exhibit reduction or abolition of seed germination in addition to a decrease in seed lipid content. The findings of the present study revealing the stearoyl-ACP thioesterase-mediated enhancement of the stearate content that is associated with reduced germination frequency of transgenic B. juncea seeds, may explain why no natural or induced stearate-rich Brassica has been found or developed. Furthermore, this study also suggests that the newly characterized MlFatB is a potential candidate gene for refined metabolic engineering strategy in B. juncea or other plant species for increasing stearate content in seed oil.
Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
Surajit
S
Bhattacharya
Adv. Lab. for Plant Genetic Engineering, Advanced Technology Development Center, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
Saheli
S
Sinha
Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
Natasha
N
Das
Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India; Adv. Lab. for Plant Genetic Engineering, Advanced Technology Development Center, Indian Institute of Technology Kharagpur, Kharagpur 721302, India. Electronic address: mrinalkmaiti@gmail.com.
Mrinal K
MK
Maiti
D017343:Genes, Plant; D018525:Germination; D050356:Lipid Metabolism; D009149:Mustard Plant / Q000235:genetics / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D012639:Seeds / Q000254:growth & development / Q000378:metabolism*; D013229:Stearic Acids / Q000378:metabolism*
D010938:Plant Oils; D010940:Plant Proteins; D013229:Stearic Acids; C031183:stearic acid
Brassica juncea (Indian mustard); Erucic acid; FatB thioesterase; Fatty acid biosynthesis; Metabolic engineering; Seed lipid; Stearic acid