Serum metabolic profile predicts adverse central haemodynamics in patients with type 2 diabetes mellitus.
OBJECTIVE
People with type 2 diabetes mellitus (T2DM) have abnormal peripheral and central haemodynamics at rest and during exercise, probably due to metabolic perturbations, but mechanisms are unknown. We used untargeted metabolomics to determine the relationships between metabolic perturbations and haemodynamics (peripheral and central) measured at rest and during exercise.


METHODS
Serum samples from 39 participants with T2DM (62 ± 9 years; 46 % male) and 39 controls (52 ± 10 years; 51 % male) were analysed by liquid chromatography-mass spectrometry, nuclear magnetic resonance spectroscopy and principal component analysis. Scores on principal components (PC) were used to assess relationships with haemodynamics including peripheral and central BP, central augmentation index (AIx) and central augmentation pressure (AP).


RESULTS
Participants with T2DM had higher resting and exercise haemodynamics (peripheral and central BP, central AIx and central AP) compared to controls (p < 0.05). PC that comprised of a signature metabolic pattern of T2DM was independently associated with resting and exercise central AIx and central AP (p < 0.05).


CONCLUSIONS
Serum metabolic profile was associated with central, but not peripheral, haemodynamics in T2DM participants, suggesting that metabolic irregularities may explain abnormal central haemodynamics in T2DM patients.
Menzies Institute for Medical Research, University of Tasmania, 17 Liverpool Street, Hobart, 7000, Australia.
Sonja B
SB
Nikolic
Centre of Human and Aerospace Physiological Sciences, King's College London, London, UK. lindsay.m.edwards@gsk.com.|Fibrosis Discovery Performance Unit, GSK Medicines Research Centre, GlaxoSmithKline R&D, Gunnels Wood Road, Stevenage, Hertfordshire, SG1 2NY, UK. lindsay.m.edwards@gsk.com.
Lindsay M
LM
Edwards
School of Mathematics and Physics, University of Tasmania, Hobart, Australia.
Yuliya V
YV
Karpievitch
Central Science Laboratory, University of Tasmania, Hobart, Australia.
Richard
R
Wilson
Central Science Laboratory, University of Tasmania, Hobart, Australia.
James
J
Horne
School of Health Sciences, University of Tasmania, Launceston, Australia.
Murray J
MJ
Adams
Menzies Institute for Medical Research, University of Tasmania, 17 Liverpool Street, Hobart, 7000, Australia. James.Sharman@utas.edu.au.
James E
JE
Sharman
D000368:Aged; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000503:physiopathology; D015444:Exercise; D005260:Female; D006439:Hemodynamics; D006801:Humans; D008297:Male; D055442:Metabolome; D008875:Middle Aged
Blood pressure; Exercise; Liquid chromatography–mass spectrometry; Metabolomics; Nuclear magnetic resonance spectroscopy