Biocomposite based on reduced graphene oxide film modified with phenothiazone and flavin adenine dinucleotide-dependent glucose dehydrogenase for glucose sensing and biofuel cell applications.
A novel composite material for the encapsulation of redox enzymes was prepared. Reduced graphene oxide film with adsorbed phenothiazone was used as a highly efficient composite for electron transfer between flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase and electrodes. Measured redox potential for glucose oxidation was lower than 0 V vs Ag/AgCl electrode. The fabricated biosensor showed high sensitivity of 42 mA M(-1) cm(-2), a linear range of glucose detection of 0.5-12 mM, and good reproducibility and stability as well as high selectivity for different interfering compounds. In a semibiofuel cell configuration, the hybrid film generated high power output of 345 μW cm(-2). These results demonstrate a promising potential for this composition in various bioelectronic applications.
Department of Life Sciences and the Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev , P.O. Box 653, Beer-Sheva 84105, Israel.
Yehonatan
Y
Ravenna
Department of Life Sciences and the Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev , P.O. Box 653, Beer-Sheva 84105, Israel.
Lin
L
Xia
The Casali Institute, The Institute of Chemistry, and The Harvey M. Krueger Family Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Jenny
J
Gun
The Casali Institute, The Institute of Chemistry, and The Harvey M. Krueger Family Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Alexey A
AA
Mikhaylov
The Casali Institute, The Institute of Chemistry, and The Harvey M. Krueger Family Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Alexander G
AG
Medvedev
The Casali Institute, The Institute of Chemistry, and The Harvey M. Krueger Family Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Ovadia
O
Lev
Department of Life Sciences and the Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev , P.O. Box 653, Beer-Sheva 84105, Israel.
Lital
L
Alfonta
D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001674:Bioelectric Energy Sources; D015374:Biosensing Techniques; D004566:Electrodes; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism*; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry* / Q000378:metabolism; D010640:Phenothiazines / Q000737:chemistry* / Q000378:metabolism
D001672:Biocompatible Materials; D010087:Oxides; D010640:Phenothiazines; D005182:Flavin-Adenine Dinucleotide; D006108:Graphite; D042843:Glucose 1-Dehydrogenase; D005947:Glucose