Migrating songbirds on stopover prepare for, and recover from, oxidative challenges posed by long-distance flight.
Managing oxidative stress is an important physiological function for all aerobic organisms, particularly during periods of prolonged high metabolic activity, such as long-distance migration across ecological barriers. However, no previous study has investigated the oxidative status of birds at different stages of migration and whether that oxidative status depends on the condition of the birds. In this study, we compared (1) energy stores and circulating oxidative status measures in (a) two species of Neotropical migrants with differing migration strategies that were sampled at an autumn stopover site before an ecological barrier; and (b) a species of trans-Saharan migrant sampled at a spring stopover site after crossing an ecological barrier; and (2) circulating oxidative measures and indicators of fat metabolism in a trans-Saharan migrant after stopovers of varying duration (0-8 nights), based on recapture records. We found fat stores to be positively correlated with circulating antioxidant capacity in Blackpoll Warblers and Red-eyed Vireos preparing for fall migration on Block Island, USA, but uncorrelated in Garden Warblers on the island of Ponza, Italy, after a spring crossing of the Sahara Desert and Mediterranean Sea. In all circumstances, fat stores were positively correlated with circulating lipid oxidation levels. Among Garden Warblers on the island of Ponza, fat anabolism increased with stopover duration while oxidative damage levels decreased. Our study provides evidence that birds build antioxidant capacity as they build fat stores at stopover sites before long flights, but does not support the idea that antioxidant stores remain elevated in birds with high fuel levels after an ecological barrier. Our results further suggest that lipid oxidation may be an inescapable hazard of using fats as the primary fuel for flight. Yet, we also show that birds on stopover are capable of recovering from the oxidative damage they have accrued during migration, as lipid oxidation levels decrease with time on stopover. Thus, the physiological strategy of migrating songbirds may be to build prophylactic antioxidant capacity in concert with fuel stores at stopover sites before a long-distance flight, and then repair oxidative damage while refueling at stopover sites after long-distance flight.
Department of Natural Resources Science, University of Rhode Island Kingston, Rhode Island.
Megan M
MM
Skrip
Institute of Environmental Sciences, Jagiellonian University Kraków, Poland.
Ulf
U
Bauchinger
Max-Planck-Institut für Ornithologie, Abteilung für Verhaltensneurobiolgie Seewiesen, Germany.
Wolfgang
W
Goymann
Department of Life Sciences and Biotechnology, University of Ferrara Ferrara, Italy.
Leonida
L
Fusani
Department of Aquatic Resources, Marine Research Institute, Swedish University of Agricultural Sciences Lysekil, Sweden.
Massimiliano
M
Cardinale
Department of Natural Resources Science, University of Rhode Island Kingston, Rhode Island.
Rebecca R
RR
Alan
Department of Natural Resources Science, University of Rhode Island Kingston, Rhode Island.
Scott R
SR
McWilliams
Antioxidant capacity; condition dependency; ecological barriers; lipid oxidation; long-distance flight; migration strategy