Analysis of trace dicyandiamide in stream water using solid phase extraction and liquid chromatography UV spectrometry.
An improved method for trace level quantification of dicyandiamide in stream water has been developed. This method includes sample pretreatment using solid phase extraction. The extraction procedure (including loading, washing, and eluting) used a flow rate of 1.0mL/min, and dicyandiamide was eluted with 20mL of a methanol/acetonitrile mixture (V/V=2:3), followed by pre-concentration using nitrogen evaporation and analysis with high performance liquid chromatography-ultraviolet spectroscopy (HPLC-UV). Sample extraction was carried out using a Waters Sep-Pak AC-2 Cartridge (with activated carbon). Separation was achieved on a ZIC(®)-Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) (50mm×2.1mm, 3.5μm) chromatography column and quantification was accomplished based on UV absorbance. A reliable linear relationship was obtained for the calibration curve using standard solutions (R(2)>0.999). Recoveries for dicyandiamide ranged from 84.6% to 96.8%, and the relative standard deviations (RSDs, n=3) were below 6.1% with a detection limit of 5.0ng/mL for stream water samples.
Department of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, China; Department of Chemistry, Mississippi State University, Mississippi State, MS 39762, USA. Electronic address: qhd324@163.com.
Huidong
H
Qiu
Department of Chemistry, Mississippi State University, Mississippi State, MS 39762, USA.
Dongdi
D
Sun
Department of Chemistry, Mississippi State University, Mississippi State, MS 39762, USA.
Sameera R
SR
Gunatilake
Department of Chemistry, Mississippi State University, Mississippi State, MS 39762, USA.
Jinyan
J
She
Department of Chemistry, Mississippi State University, Mississippi State, MS 39762, USA. Electronic address: mushdq@gmail.com.
Todd E
TE
Mlsna
D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000379:methods*; D006146:Guanidines / Q000032:analysis*; D057230:Limit of Detection; D045483:Rivers / Q000737:chemistry*; D052616:Solid Phase Extraction; D013056:Spectrophotometry, Ultraviolet; D014874:Water Pollutants, Chemical / Q000032:analysis*
D006146:Guanidines; D014874:Water Pollutants, Chemical; C004711:dicyandiamido
Dicyandiamide; High performance liquid chromatography–ultraviolet spectrometry; Solid phase extraction; Stream water samples