Automated cold temperature cycling improves in vitro platelet properties and in vivo recovery in a mouse model compared to continuous cold storage.
BACKGROUND
Platelets (PLTs) stored at cold temperatures (CTs) for prolonged time have dramatically reduced bacterial growth but poor survival when infused. A previous study demonstrated that human PLTs stored with manual cycling between 4 °C (12 hr) and 37 °C (30 min) and infused into severe combined immunodeficient (SCID) mice had survivals similar to or greater than those stored at room temperature (RT). In this study, the in vitro and in vivo properties of PLTs stored in an automated incubator programmed to cycle between 5 °C (11 hr) and 37 °C (1 hr) were evaluated.


METHODS
A Trima apheresis unit (n = 12) was aliquoted (60 mL) in CLX bags. One sample was stored with continuous agitation (RT), a second sample was stored at 4-6 °C without agitation (CT), and a third sample was placed in an automated temperature cycler with 5 minutes of agitation during the warm-up period (thermocycling [TC]). PLTs were assayed for several relevant quality variables. On Day 7, PLTs were infused into SCID mice and in vivo recovery was assessed at predetermined time points after transfusion.


RESULTS
The glucose consumption rate, morphology score, hypotonic shock recovery level, and aggregation levels were increased and mitochondrial reactive oxygen species accumulations were decreased in TC-PLTs compared to those of CT-PLTs. The pH and Annexin V binding were comparable to those of RT-PLTs. All TC-PLTs had greater recovery than CT-PLTs and were comparable to RT-PLTs.


CONCLUSIONS
PLTs stored under automated TC conditions have improved in vivo recovery and improved results for a number of in vitro measures compared to CT-PLTs.
American Red Cross Biomedical Services, Holland Laboratory, Rockville, Maryland.
Andrey
A
Skripchenko
Laboratory of Cellular Hematology.
Monique P
MP
Gelderman
American Red Cross Biomedical Services, Holland Laboratory, Rockville, Maryland.
Helen
H
Awatefe
American Red Cross Biomedical Services, Holland Laboratory, Rockville, Maryland.
Annette
A
Turgeon
American Red Cross Biomedical Services, Holland Laboratory, Rockville, Maryland.
Dedeene
D
Thompson-Montgomery
Office of Biostatistics and Epidemiology, Center for Biologics Evaluation and Research (CBER), FDA, Silver Spring, Maryland.
Chunrong
C
Cheng
Laboratory of Cellular Hematology.
Jaroslav G
JG
Vostal
American Red Cross Biomedical Services, Holland Laboratory, Rockville, Maryland.
Stephen J
SJ
Wagner
D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology*; D001793:Blood Preservation / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D017713:Platelet Transfusion; D010983:Plateletpheresis