Fiber composite slices for multiplexed immunoassays.
Fabrication methods for the development of multiplexed immunoassay platforms primarily depend on the individual functionalization of reaction chambers to achieve a heterogeneous reacting substrate composition, which increases the overall manufacturing time and cost. Here, we describe a new type of low-cost fabrication method for a scalable immunoassay platform based on cotton threads. The manufacturing process involves the fabrication of functionalized fibers and the arrangement of these fibers into a bundle; this bundle is then sectioned to make microarray-like particles with a predefined surface architecture. With these sections, composed of heterogeneous thread fragments with different types of antibodies, we demonstrated quantitative and 7-plex immunoassays. We expect that this methodology will prove to be a versatile, low-cost, and highly scalable method for the fabrication of multiplexed bioassay platforms.
Nano Systems Institute, Seoul National University , Seoul 151-744, South Korea.
Jiyun
J
Kim
Sangwook
S
Bae
Seowoo
S
Song
QuantaMatrix Inc., Se oul National University , Seoul 151-744, South Korea.
Keumsim
K
Chung
Sunghoon
S
Kwon