[Validation Study on a Method of Determination of Nosiheptide in Formula Feeds by HPLC-FL].
A determination method of nosiheptide in formula feeds by HPLC-FL was developed and validated, including an inter-laboratory study. Formula feeds were extracted with acetone after adding acetic acid. Liquid chromatographic separation was performed using a ZORBAX Eclipse XDB-C18 column, with acetonitrile and water containing acetic acid as the mobile phase. Detection of NH was carried out with a fluorescence detector. Recovery tests and an inter-laboratory study were conducted using chicken and swine formula feeds fortified with nosiheptide at 0.5-27 mg-potency/kg. Mean recoveries in recovery tests ranged from 91.4 to 103%, and the repeatability in terms of relative standard deviation was within 7.8%. Mean recoveries of the inter-laboratory study ranged from 98.4 to 108%, the repeatability and reproducibility in terms of the relative standard deviations were within 8.1% and 13% respectively, and the HorRat values ranged from 0.21 to 0.75.
Food and Agricultural Materials Inspection Center.
Toshiaki
T
Yamata
Chisa
C
Shimamura
Miyuki
M
Asao
Norio
N
Aita
Tetsuo
T
Chihara
D000821:Animal Feed / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D007753:Laboratories; D015588:Observer Variation; D015203:Reproducibility of Results; D013844:Thiazoles / Q000032:analysis / Q000302:isolation & purification
D013844:Thiazoles; C014134:nosiheptide