Targeted delivery of liquid microvolumes into the lung.
The ability to deliver drugs to specific sites in the lung could radically improve therapeutic outcomes of a variety of lung diseases, including cystic fibrosis, severe bronchopneumonia, chronic obstructive pulmonary disease, and lung cancer. Using conventional methods for pulmonary drug administration, precise, localized delivery of exact doses of drugs to target regions remains challenging. Here we describe a more controlled delivery of soluble reagents (e.g., drugs, enzymes, and radionuclides) in microvolume liquid plugs to targeted branches of the pulmonary airway tree: upper airways, small airways (bronchioles), or the most distal alveoli. In this approach, a soluble liquid plug of very small volume (<1 mL) is instilled into the upper airways, and with programmed air ventilation of the lungs, the plug is pushed into a specific desired (more distal) airway to achieve deposition of liquid film onto the lung epithelium. The plug volume and ventilation conditions were determined by mathematical modeling of plug transport in a tubular geometry, and targeted liquid film deposition was demonstrated in rat lungs by three different in vivo imaging modalities. The experimental and modeling data suggest that instillation of microvolumes of liquid into a ventilated pulmonary airway could be an effective strategy to deliver exact doses of drugs to targeted pathologic regions of the lung, especially those inaccessible by bronchoscopy, to increase in situ efficacy of the drug and minimize systemic side effects.
Department of Biomedical Engineering, Columbia University, New York, NY 10027;
Jinho
J
Kim
Department of Biomedical Engineering, Columbia University, New York, NY 10027;
John D
JD
O'Neill
Department of Pediatrics, Columbia University, New York, NY 10029;
N Valerio
NV
Dorrello
Department of Surgery, Columbia University, New York, NY 10032;
Matthew
M
Bacchetta
Department of Biomedical Engineering, Columbia University, New York, NY 10027; Department of Medicine, Columbia University, New York, NY 10032 gv2131@columbia.edu.
Gordana
G
Vunjak-Novakovic
D000818:Animals; D016503:Drug Delivery Systems; D008168:Lung / Q000502:physiology*; D008297:Male; D008856:Microscopy, Fluorescence; D008962:Models, Theoretical; D011650:Pulmonary Alveoli / Q000502:physiology; D011663:Pulmonary Surfactants / Q000008:administration & dosage*; D012123:Pulmonary Ventilation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015656:Respiratory Mechanics / Q000502:physiology; D012137:Respiratory System / Q000187:drug effects
D011663:Pulmonary Surfactants
alveoli; liquid instillation; lung airway; lung disease; pulmonary drug delivery