Demineralized Bone Matrix Injection in Consolidation Phase Enhances Bone Regeneration in Distraction Osteogenesis via Endochondral Bone Formation.
BACKGROUND
Distraction osteogenesis (DO) is a promising tool for bone and tissue regeneration. However, prolonged healing time remains a major problem. Various materials including cells, cytokines, and growth factors have been used in an attempt to enhance bone formation. We examined the effect of percutaneous injection of demineralized bone matrix (DBM) during the consolidation phase on bone regeneration after distraction.


METHODS
The immature rabbit tibial DO model (20 mm length-gain) was used. Twenty-eight animals received DBM 100 mg percutaneously at the end of distraction. Another 22 animals were left without further procedure (control). Plain radiographs were taken every week. Postmortem bone dual-energy X-ray absorptiometry and micro-computed tomography (micro-CT) studies were performed at the third and sixth weeks of the consolidation period and histological analysis was performed.


RESULTS
The regenerate bone mineral density was higher in the DBM group when compared with that in the saline injection control group at the third week postdistraction. Quantitative analysis using micro-CT revealed larger trabecular bone volume, higher trabecular number, and less trabecular separation in the DBM group than in the saline injection control group. Cross-sectional area and cortical thickness at the sixth week postdistraction, assessed using micro-CT, were greater in the regenerates of the DBM group compared with the control group. Histological evaluation revealed higher trabecular bone volume and trabecular number in the regenerate of the DBM group. New bone formation was apparently enhanced, via endochondral ossification, at the site and in the vicinity of the injected DBM. DBM was absorbed slowly, but it remained until the sixth postoperative week after injection.


CONCLUSIONS
DBM administration into the distraction gap at the end of the distraction period resulted in a significantly greater regenerate bone area, trabecular number, and cortical thickness in the rabbit tibial DO model. These data suggest that percutaneous DBM administration at the end of the distraction period or in the early consolidation period may stimulate regenerate bone formation and consolidation in a clinical situation with delayed bone healing during DO.
Department of Orthopedic Surgery, Seoul National University Hospital, Seoul, Korea.
Ji-Beom
JB
Kim
Department of Orthopedic Surgery, Seoul National University Hospital, Seoul, Korea.
Dong Yeon
DY
Lee
Department of Orthopedic Surgery, Seoul National University Hospital, Seoul, Korea.
Sang Gyo
SG
Seo
Department of Orthopedic Surgery, Seoul National University Hospital, Seoul, Korea.
Eo Jin
EJ
Kim
Department of Orthopedic Surgery, Seoul National University Hospital, Seoul, Korea.
Ji Hye
JH
Kim
Department of Orthopedic Surgery, Seoul National University Hospital, Seoul, Korea.
Won Joon
WJ
Yoo
Department of Orthopedic Surgery, Seoul National University Hospital, Seoul, Korea.
Tae-Joon
TJ
Cho
Department of Orthopedic Surgery, Seoul National University Hospital, Seoul, Korea.
In Ho
IH
Choi
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000008:administration & dosage* / Q000494:pharmacology*; D004195:Disease Models, Animal; D006801:Humans; D007267:Injections; D008297:Male; D019857:Osteogenesis, Distraction / Q000379:methods*; D011817:Rabbits; D011859:Radiography; D013977:Tibia / Q000000981:diagnostic imaging / Q000601:surgery
D018786:Bone Substitutes
Bone development; Bone substitute; Distraction; Osteogenesis