Regulatory Impact of Amniotic Membrane Transplantation on Presence of Adhesion/Growth-Regulatory Galectins-1 and -7 in Corneal Explants from Acanthamoeba Keratitis Patients: Clinical Note.
To assess the impact of Acanthamoeba keratitis (AK) and amniotic membrane transplantation (AMT) in corneal explants on presence of two multifunctional endogenous lectins, i.e. galectins-1 and -7.



Ten corneal explants from AK patients (five with previous AMT and five controls without this treatment) and seven specimens of disease-free control cornea were processed by indirect fluorescent immunohistochemistry.



Immunostaining for both galectins was obtained in the epithelium, stroma and the endothelial layer of all controls, with the strongest positivity in the epithelium. Significantly decreased intensity for galectin-1 was recorded in the epithelium of corneal explants from patients with AK and AMT. The signal for galectin-7 was significantly decreased in the epithelium of AK patients and normalized after AMT.



AMT has a marked impact on presence of the two galectins in opposite directions, encouraging complete profiling for this family of endogenous effectors.
a First Faculty of Medicine, Institute of Histology and Embryology, Charles University in Prague , Albertov , Prague , Czech Republic .
Natalia
N
Smorodinova
b Faculty of Veterinary Medicine , Institute of Physiological Chemistry, Ludwig-Maximilians-University Munich , Veterinärstrasse, Munich , Germany .
Herbert
H
Kaltner
c Laboratory of the Biology and Pathology of the Eye , Department of Inherited Metabolic Disorders, First Faculty of Medicine and General Teaching Hospital, Charles University in Prague , Ke Karlovu , Prague , Czech Republic .
Katerina
K
Jirsová
d Department of Ophthalmology , First Faculty of Medicine, Charles University in Prague , U Nemocnice , Prague .|e Lexum European Eye Clinic , Antala Staška, Prague , Czech Republic and.
Enkela
E
Hrdličková-Cela
b Faculty of Veterinary Medicine , Institute of Physiological Chemistry, Ludwig-Maximilians-University Munich , Veterinärstrasse, Munich , Germany .
Sabine
S
André
a First Faculty of Medicine, Institute of Histology and Embryology, Charles University in Prague , Albertov , Prague , Czech Republic .
Tomáš
T
Kučera
f First Faculty of Medicine, Institute of Anatomy, Charles University in Prague , U Nemocnice , Prague , Czech Republic.
Karel
K
Smetana
b Faculty of Veterinary Medicine , Institute of Physiological Chemistry, Ludwig-Maximilians-University Munich , Veterinärstrasse, Munich , Germany .
Hans-Joachim
HJ
Gabius
D015823:Acanthamoeba Keratitis / Q000378:metabolism / Q000601:surgery*; D000328:Adult; D000650:Amnion / Q000637:transplantation*; D001684:Biological Dressings; D015415:Biomarkers / Q000378:metabolism; D003315:Cornea / Q000378:metabolism* / Q000601:surgery; D015822:Eye Infections, Parasitic / Q000175:diagnosis / Q000378:metabolism / Q000601:surgery*; D005260:Female; D037483:Galectin 1; D037161:Galectins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D015948:Keratoplasty, Penetrating / Q000379:methods*; D008297:Male; D008875:Middle Aged
D015415:Biomarkers; D037483:Galectin 1; D037161:Galectins; C092525:LGALS7 protein, human
Agglutinin; cornea; immunohistochemistry; keratoplasty; lectin