Bioinformatics Analysis to Identify RNA-Protein Interactions in Oogenesis.
Hundreds of RNA-binding proteins are known, but the biological functions are known for only a few of them. They regulate various aspects of RNA processing or biogenesis such as splicing, polyadenylation, and translation. Here I describe a bioinformatics approach that we developed to identify potential new mRNA target(s) of the Drosophila master sex-switch protein Sex-lethal (SXL) by combining computational analysis with genetic and biochemical investigation. This approach could be used to identify new RNA-protein interactions during oogenesis in the female germline and should be applicable to numerous other posttranscriptional regulatory events.
Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Boulder, CO, 80309, USA, rsingh@colorado.edu.
Ravinder
R
Singh
D000818:Animals; D019295:Computational Biology / Q000379:methods*; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster; D005260:Female; D018507:Gene Expression Regulation, Developmental; D009866:Oogenesis; D014176:Protein Biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*
D029721:Drosophila Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C067777:Sxl protein, Drosophila