Swainsonine exposure induces impairment of host immune response in pregnant BALB/c mice.
BACKGROUND
Swainsonine can cause serious disorders in reproduction of livestock, affecting both corpora lutea and reproductive hormone. The purpose of this study was to investigate the mechanisms of swainsonine about the immunotoxic effects on pregnant mice in vivo.


RESULTS
The peripheral Th1/Th2 was detected by Ionomycin and phorbol myristate acetate (PMA)-stimulating peripheral blood mononuclear cells (PBMC) of phase pregnant mice. Relevant cytokines in serum was evaluated after exposed to different dose of swainsonine. Gene expression of IL-1β, IFN-γ, TNF-α, IL-4 and IL-10 in PBMC was assessed by real-time PCR. Swainsonine caused vacuolization phenomenon of lutein cells and a dose-effect relationship. The IL-1β, IFN-γ and TNF-α were promoted, but IL-4 and IL-10 were suppressed in serum. Swainsonine significantly increased IL-1β, IFN-γ and TNF-α nuclear translocation and decreased IL-4 and IL-10. Swainsonine resulted in a significant shift of peripheral Th1/Th2 paradigm to Th1.


CONCLUSIONS
Our data demonstrate that the immunomodulatory of swainsonine disturbed the regular immunologic state of the pregnant mice. This may increase the risk of abortion and probably resulted in serious disorders in reproduction of livestock.
Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P.R. China. hychun114@163.com.|Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 611130, P.R. China. hychun114@163.com.
Yanchun
Y
Hu
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 611130, P.R. China. Woo.ray2@gmail.com.
Lei
L
Wu
Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P.R. China. wangcm@ioz.ac.cn.
Chengmin
C
Wang
Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P.R. China. luojing@ioz.ac.cn.
Jing
J
Luo
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 611130, P.R. China. 381404719@qq.com.
Fei
F
Liao
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 611130, P.R. China. 82193514@qq.com.
Hui
H
Tan
Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P.R. China. hehx@ioz.ac.cn.
Hongxuan
H
He
D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D007109:Immunity / Q000187:drug effects*; D014975:Lutein / Q000378:metabolism; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D017026:Swainsonine / Q000494:pharmacology*; D018417:Th1 Cells / Q000187:drug effects / Q000378:metabolism; D018418:Th2 Cells / Q000187:drug effects / Q000378:metabolism
D016207:Cytokines; D017026:Swainsonine; D014975:Lutein