Structure, physicochemical and biological properties of new complex salt of aqua-(nitrilotriacetato-N,O,O',O")-oxidovanadium(IV) anion with 1,10-phenanthrolinium cation.
The crystal structure of new 1,10-phenathrolin-1-ium aqua-(nitrilotriacetato-N,O,O',O")-oxidovanadium(IV) semihydrate of molecular formula (phenH)[VO(NTA)(H2O)]∙1/2H2O was determined. This is the first example of structurally characterized compound that comprises a distinctly separated, monomeric [VO(NTA)(H2O)](-) coordination entity. The crystallographic measurements have subsequently been complemented by the IR spectroscopic characterization and thermal analysis. Furthermore, the electrochemical (cyclic voltammetry) as well as spectrophotometric (UV-vis) studies revealed that the compound is capable of scavenging the superoxide free radicals (O2(-)) as well as stable organic radicals i.e. 2,2'-azinobis(3-ethylbenzothiazoline-6 sulfonic acid) cation radical (ABTS(+)) and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), but its reactivity towards radicals is lower than that of VOSO4. Finally, biological properties of the complex in the range of 1-100 μM were investigated in relation to its cytoprotective activity against the oxidative damage generated exogenously by using hydrogen peroxide in the hippocampal neuronal HT22 cell line (the MTT and LDH tests). It has been established that in contrast to VOSO4 the title compound protects the HT22 from the oxidative damage. The paper presents a new perspective for oxidovanadium(IV) complexes as candidates for novel, low-molecular mass cytoprotective agents.
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland.
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Tesmar
Department of Medical Chemistry, Medical University of Gdańsk, Dębinki 1, 80-211 Gdańsk, Poland.
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Inkielewicz-Stępniak
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland.
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Sikorski
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland. Electronic address: dariusz.wyrzykowski@ug.edu.pl.
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Wyrzykowski
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland.
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Jacewicz
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland.
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Zięba
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland.
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Pranczk
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland.
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Ossowski
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland.
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Chmurzyński
D000818:Animals; D002460:Cell Line; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000138:chemical synthesis* / Q000494:pharmacology; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000494:pharmacology; D018384:Oxidative Stress
D056831:Coordination Complexes; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D009942:Organometallic Compounds; C000611420:aqua(nitrilotriacetato-N,O,O',O'')oxidovanadium(IV)
Cytoprotective activity; Free radicals; Hippocampal HT22 cells; Vanadium complexes