Synthesis, Characterization, and Magnetic Resonance Evaluation of Polyglycerol-Functionalized Detonation Nanodiamond Conjugated with Gadolinium(III) Complex.
A nanodiamond-polyglycerol-gadolinium(ll) conjugate has been designed and prepared as novel nanodiamond-based magnetic resonance (MR) contrast agent dispersible in physiological media. Detonation nanodiamond (dND) was first grafted with polyglycerol (PG) through ring-opening polymerization of glycidol to impart dispersibility to dND in physiological media. Since the hydroxyl group in PG serves as a scaffold for further surface functionalization, diethylenetriaminepentaacetic acid (DTPA) was immobilized on the surface of dND-PG through multistep organic transformations and Gd(III) ion was complexed in the last step. The resulting dND-PG-Gd(III) exhibited good dispersibility (> 4.5 mg/mL) and stability (> 3 months) in phosphate buffered saline (PBS). In vitro MR evaluation indicates that water proton T1 relaxivity or r1 of dND-PG-Gd(III) in aqueous solutions is larger than that of Magnevist® and the difference in the relaxivity becomes larger under weaker magnetic fields. The good dispersibility together with relatively high T1 relaxivity makes dND-PG-Gd(III) a promising contrast agent for in vivo MR imaging.
Li
L
Zhao
Akihiko
A
Shiino
Hongmei
H
Qin
Takahide
T
Kimura
Naoki
N
Komatsu
D004058:Diffusion; D005682:Gadolinium / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000295:instrumentation / Q000379:methods*; D008422:Materials Testing; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D058726:Nanoconjugates / Q000737:chemistry* / Q000648:ultrastructure; D058612:Nanodiamonds / Q000737:chemistry*; D019047:Phantoms, Imaging; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D053769:Nanocapsules; D058726:Nanoconjugates; D058612:Nanodiamonds; D011108:Polymers; C043941:polyglycerol; D005682:Gadolinium; D005990:Glycerol