Pharmacokinetics Evaluation of Carbon Nanotubes Using FTIR Analysis and Histological Analysis.
Carbon nanotubes (CNTs) are biologically non-toxic and long-circulating nanostructures that have special physical properties. This study was focused on developing alternative methods that track carbon nanotubes, like FR-IR to classical tissue histological procedure. FT-IR absorption spectra were used to confirm the carboxylation of carbon nanotubes and to evaluate the presence of carbon nanotubes from bulk spleen samples and histologically prepared samples (control spleen and spleen with SWCNT-COOH). FT-IR spectrum of spleen sample from animals injected with CNTs shows major spectral differences consisting in infrared bands located at ~1173 cm(-1), ~ 1410 cm(-1); ~1658 cm(-1), ~1737 cm(-1) and around 1720 cm(-1) respectively. In terms of localization of carbon nanotubes, selective accumulation of marginal zone macrophages and splenic red pulp is observed for all treated groups, indicating the presence of carbon nanotubes even at 3, 4 and 7 days after treatment. In summary, we believe that histological evaluation and FT-IR can provide more characteristic information about the pharmacokinetcis and the clearance of carbon nanotubes.
Claudia
C
Gherman
Matea Cristian
MC
Tudor
Bele
B
Constantin
Tabaran
T
Flaviu
Razvan
R
Stefan
Bindea
B
Maria
Sergiu
S
Chira
Cornelia
C
Braicu
Laura
L
Pop
Roxana Cojocneanu
RC
Petric
Ioana
I
Berindan-Neagoe
D000818:Animals; D002244:Carbon / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D006651:Histocytochemistry; D007274:Injections, Intraperitoneal; D008264:Macrophages / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D037742:Nanotubes, Carbon / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013154:Spleen / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism
D037742:Nanotubes, Carbon; D002244:Carbon