Biogeographic pattern of genetic diversity detected by RAPD and ISSR analysis in Gypsophila (Caryophyllaceae) species from Turkey.
Gypsophila L. is the 3rd-largest genus of Caryophyllaceae in Turkey, a country that includes 60 taxa belonging to 56 species of the 126 recognized in the genus. A total of 35 taxa are endemic to Turkey, with an approximately 60% endemism ratio. In this study, the genetic diversity of 14 Gypsophila species from Turkey was analyzed using random amplification of polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Sixteen RAPD and 6 ISSR primers produced 132 polymorphic bands. RAPD, ISSR, and RAPD + ISSR primers for the 14 species showed 92.7, 93.8, and 92.9% polymorphism, respectively. Our results indicate that RAPD and ISSR markers are reliable and effective for assessing the genetic diversity of Gypsophila species. Gypsophila species studied were separated into 2 clusters. Our analysis shows that the clusters correlated with geographic and phytogeographic regions.
Department of Biology, Faculty of Science and Arts, Erzincan University, Erzincan, Turkey.
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M
Korkmaz
Department of Biology, Faculty of Science and Arts, Erzincan University, Erzincan, Turkey nyildirim@erzincan.edu.tr.
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NY
Dogan
D029748:Caryophyllaceae / Q000235:genetics*; D017931:DNA Primers; D014644:Genetic Variation; D018895:Microsatellite Repeats; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D013045:Species Specificity; D014421:Turkey
D017931:DNA Primers