Proanthocyanidin and fish oil potent activity against cisplatin-induced renal cell cycle arrest and apoptosis in rats.
Cisplatin is an effective chemotherapeutic agent that displays dose-limiting nephrotoxicity. In the present study, the efficacy of grape seed proanthocyanidin extract (GSPE: 100 mg/kg/day) and fish oil (FO: 5 mL/kg/day) against cisplatin-induced nephrotoxicity was evaluated in terms of DNA damage, histopathological changes and expression levels of molecular markers of apoptosis. The administration of cisplatin (CP) (7 mg/kg) results in an increasing percentage of S-phase, G2/M and apoptosis. Furthermore, CP induces apoptosis as indicated by an elevation of renal caspase-3 and reduction in the expression of BCL-2. In addition to occurred renal histopathological changes as manifested by tubular degeneration, degenerative glomerulus, necrotic tubular cells, and cell debris. On the other hand, the administration of GSPE or FO pre-cisplatin treatment can be ameliorated the current DNA cell cycle alterations by the restoration of expression of proteins related to apoptosis and reduced the undesirable renal histopathological changes. So, it can be concluded that the consumption of GSPE or FO might be useful for minimizing nephrotoxicity caused by cisplatin chemotherapy through their anti-apoptotic and antioxidant properties.
a Department of Zoology, Physiology Division , Faculty of Science, Mansoura University , Mansoura , Egypt and.
Hanaa A
HA
Hassan
a Department of Zoology, Physiology Division , Faculty of Science, Mansoura University , Mansoura , Egypt and.
Gamal M
GM
Edrees
b Immunology Division, Urology and Nephrology Center , Mansoura University , Mansoura , Egypt.
Ezz M
EM
El-Gamel
a Department of Zoology, Physiology Division , Faculty of Science, Mansoura University , Mansoura , Egypt and.
Elsamra A
EA
El-Sayed
D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects*; D000975:Antioxidants / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D001806:Blood Urea Nitrogen; D053148:Caspase 3 / Q000187:drug effects; D059447:Cell Cycle Checkpoints / Q000187:drug effects* / Q000235:genetics; D002945:Cisplatin / Q000009:adverse effects*; D004249:DNA Damage / Q000187:drug effects; D005395:Fish Oils / Q000627:therapeutic use*; D056604:Grape Seed Extract / Q000627:therapeutic use*; D007684:Kidney Tubules / Q000473:pathology; D008297:Male; D044945:Proanthocyanidins / Q000627:therapeutic use*; D051381:Rats
D000970:Antineoplastic Agents; D000975:Antioxidants; D005395:Fish Oils; D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D044945:Proanthocyanidins; C505410:Casp3 protein, rat; D053148:Caspase 3; D002945:Cisplatin
Cisplatin; DNA damage; FO; GSPE; nephrotoxicity apoptosis