In vitro evaluation on HeLa cells of protective mechanisms of probiotic lactobacilli against Candida clinical isolates.
OBJECTIVE
To characterize in vitro the ability of human Lactobacillus strains to inhibit the adhesion, to displace and to compete with clinically isolated Candida strains.


RESULTS
Three types of assays were performed to determine the inhibitory effect of Lactobacillus plantarum 319, Lactobacillus rhamnosus IMC 501, Lactobacillus paracasei IMC 502 and a specific probiotic combination (SYNBIO) on adhesion of Candida pathogens to HeLa cells: blockage by exclusion (lactobacilli and HeLa followed by pathogens), competition (lactobacilli, HeLa and pathogens together) and displacement (pathogens and HeLa followed by the addition of lactobacilli). Bacterial adhesion to HeLa was quantified by microscopy after May-Grünwald/Giemsa stain. The inhibition results highlight a significant (P < 0·05) competition of the considered probiotics against all the Candida strains. The results suggest that the probiotic strains used in this study could prevent colonization of the urogenital tract by relevant pathogens such as Candida strains through barrier and interference mechanisms (mainly displacement and competition), but the degree of inhibition of adhesion was bacterial strain-dependent.


CONCLUSIONS
The results support the potential of these Lactobacillus probiotic strains as anti-infective agents in the vagina and encourage further studies about their capacity to prevent and manage urogenital tract infections in females.


CONCLUSIONS
To optimize the defensive properties of the vaginal microbiota, improving the health of many women by probiotic intervention.
School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.|School of Advanced Studies, University of Camerino, Camerino, Italy.
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Coman
School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.|Synbiotec S.r.l., Spin-off of UNICAM, Camerino, Italy.
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Verdenelli
School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.|Synbiotec S.r.l., Spin-off of UNICAM, Camerino, Italy.
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Cecchini
School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.|Synbiotec S.r.l., Spin-off of UNICAM, Camerino, Italy.
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Silvi
School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.|Synbiotec S.r.l., Spin-off of UNICAM, Camerino, Italy.
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Orpianesi
CSL Centro Sperimentale del Latte, Lodi, Italy.
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Caspani
Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Rome, Italy.
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Mondello
School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.|Synbiotec S.r.l., Spin-off of UNICAM, Camerino, Italy.
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Cresci
D001422:Bacterial Adhesion; D002175:Candida / Q000187:drug effects / Q000502:physiology*; D002177:Candidiasis / Q000188:drug therapy / Q000382:microbiology*; D004353:Drug Evaluation, Preclinical; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007778:Lactobacillus / Q000502:physiology*; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D052201:Lacticaseibacillus rhamnosus / Q000502:physiology*; D019936:Probiotics / Q000494:pharmacology*; D014621:Vagina / Q000382:microbiology
Candida infection; HeLa cell culture; inhibition mechanisms; lactobacilli; probiotics