Factors associated with real-time RT-PCR cycle threshold values among medically attended influenza episodes.
We evaluated the cycle threshold (CT) values of 1,160 influenza A positive and 806 influenza B positive specimens from two seasons of the US Flu VE Network to identify factors associated with CT values. Low CT values (high genomic load) were associated with shorter intervals between illness onset and specimen collection, young age (ages 3-8 years old), and self-rated illness severity for both influenza A and B. Low CT values were also associated with reported fever/feverishness and age ≥65 years for influenza A.
Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia.|Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee.
Sarah
S
Spencer
Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia.|Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee.
Jessie
J
Chung
Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia.
Mark
M
Thompson
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas.|Department of Pediatrics, Baylor College of Medicine, Houston, Texas.
Pedro A
PA
Piedra
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas.
Alan
A
Jewell
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas.
Vasanthi
V
Avadhanula
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas.
Minghua
M
Mei
Group Health Research Institute, Seattle Washington.
Michael L
ML
Jackson
Marshfield Clinic Research Foundation, Marshfield Wisconsin.
Jennifer
J
Meece
Marshfield Clinic Research Foundation, Marshfield Wisconsin.
Maria
M
Sundaram
Marshfield Clinic Research Foundation, Marshfield Wisconsin.
Edward A
EA
Belongia
Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, Michigan.
Rachel
R
Cross
Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, Michigan.
Emileigh
E
Johnson
Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania.
Arlene
A
Bullotta
Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania.
Charles
C
Rinaldo
Scott and White Healthcare, Temple, Texas.
Manjusha
M
Gaglani
Scott and White Healthcare, Temple, Texas.
Kempapura
K
Murthy
Scott and White Healthcare, Temple, Texas.
Lydia
L
Clipper
Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia.
LaShondra
L
Berman
Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia.
Brendan
B
Flannery
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D009981:Influenza B virus / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000473:pathology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D019562:Viral Load; D055815:Young Adult
Influenza; RT-PCR; cycle threshold value