Characterization of endophytic Bacillus strains from tomato plants (Lycopersicon esculentum) displaying antifungal activity against Botrytis cinerea Pers.
Eighty endophytic bacteria were isolated from healthy tissues of roots, stems, leaves and fruits of tomato plants (Lycopersicon esculentum). Four strains, named BL1, BT5, BR8 and BF11 were selected for their antagonism against Botrytis cinerea, a phytopathogenic fungus responsible of gray mold in several important crops, with growth inhibitory activity ranging from 27 to 53%. Morphological, biochemical, and molecular parameters as 16S rDNA sequencing demonstrated that the selected bacterial strains were related to Bacillus species which are known to produce and secrete a lot of lipopeptides with strong inhibitory effect against pathogen mycelial growth. Electrospray mass spectrometry analysis showed that these strains produced heterogeneous mixture of antibiotics belonging to fengycin and surfactin for BL1 and BT5, to iturin and surfactin for BR8, to bacillomycin D, fengycin and surfactin for BF11. Furthermore, these bacteria exhibited biocontrol potential by reducing the disease severity when tested on detached leaflets. Based on their antifungal activity against Botrytis cinerea, these strains could be used for biological control of plant diseases.
Laboratory of Bioactive Substances, Biotechnology Center of Borj-Cedria, BP 901, 2050, Hammam-Lif, Tunisia.
Asma
A
Kefi
Laboratory of Bioactive Substances, Biotechnology Center of Borj-Cedria, BP 901, 2050, Hammam-Lif, Tunisia.
Imen
I
Ben Slimene
Laboratory of Bioactive Substances, Biotechnology Center of Borj-Cedria, BP 901, 2050, Hammam-Lif, Tunisia.
Ines
I
Karkouch
PBS Laboratory, UMR 6270 CNRS, FR 3038, Proteomic Platform PISSARO, Institute for Research and Innovation in Biomedicine, University of Rouen, 76821, Mont-Saint-Aignan Cedex, France.
Christophe
C
Rihouey
Laboratory of Bioactive Substances, Biotechnology Center of Borj-Cedria, BP 901, 2050, Hammam-Lif, Tunisia.
Sana
S
Azaeiz
Laboratory of Bioactive Substances, Biotechnology Center of Borj-Cedria, BP 901, 2050, Hammam-Lif, Tunisia.
Marwa
M
Bejaoui
Laboratory of Bioactive Substances, Biotechnology Center of Borj-Cedria, BP 901, 2050, Hammam-Lif, Tunisia.
Rania
R
Belaid
PBS Laboratory, UMR 6270 CNRS, FR 3038, Proteomic Platform PISSARO, Institute for Research and Innovation in Biomedicine, University of Rouen, 76821, Mont-Saint-Aignan Cedex, France.
Pascal
P
Cosette
PBS Laboratory, UMR 6270 CNRS, FR 3038, Proteomic Platform PISSARO, Institute for Research and Innovation in Biomedicine, University of Rouen, 76821, Mont-Saint-Aignan Cedex, France.
Thierry
T
Jouenne
Laboratory of Bioactive Substances, Biotechnology Center of Borj-Cedria, BP 901, 2050, Hammam-Lif, Tunisia. limam_ferid@yahoo.fr.
Ferid
F
Limam
D000898:Antibiosis; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D001407:Bacillus / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D001483:Base Sequence; D061046:Biological Control Agents; D020171:Botrytis / Q000737:chemistry / Q000502:physiology*; D004275:DNA, Ribosomal / Q000737:chemistry; D060026:Endophytes; D055666:Lipopeptides / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D018551:Solanum lycopersicum / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000494:pharmacology; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology / Q000628:therapy; D018515:Plant Leaves / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D018547:Plant Stems / Q000382:microbiology; D021241:Spectrometry, Mass, Electrospray Ionization
D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D061046:Biological Control Agents; D004275:DNA, Ribosomal; D055666:Lipopeptides; D010455:Peptides; C049972:fengycin; C027845:bacillomycin D
Antifungal activity; Bacillus sp.; Botrytis cinerea; Endophytic bacteria; Lipopeptides; Tomato