Association between Genetic Polymorphisms of DNA Repair Genes and Chromosomal Damage for 1,3-Butadiene-Exposed Workers in a Matched Study in China.
The aim of the study was to examine the association between polymorphisms of DNA repair genes and chromosomal damage of 1,3-butadiene- (BD-) exposed workers. The study was conducted in 45 pairs of occupationally exposed workers in a BD product workshop and matched control workers in an administrative office and a circulatory water workshop in China. Newly developed biomarkers (micronuclei, MNi; nucleoplasmic bridges, NPBs; nuclear buds, NBUDs) in the cytokinesis-blocked micronucleus (CBMN) cytome assay were adopted to detect chromosomal damage. PCR and PCR-restriction fragment length polymorphism (RFLP) are adopted to analyze polymorphisms of DNA repair genes, such as X-ray repair cross-complementing Group 1 (XRCC1), O6-methylguanine-DNA methyltransferase (MGMT), poly (adenosine diphosphate-ribose) polymerases (ADPRT), and apurinic/apyrimidinic endonucleases (APE1). The BD-exposed workers exhibited increased frequencies of MNi and NPBs when compared to subjects in the control group. The results also show that the BD-exposed workers carrying XRCC1 diplotypes TCGA-CCGG (4.25 ± 2.06 ‰) (FR = 2.10, 95% CI: 1.03-4.28) and TCGG-TCGA (5.80 ± 3.56 ‰) (FR = 2.75, 95% CI: 0.76-2.65) had statistically higher NBUD frequencies than those who carried diplotype TCGG-TCGG (1.89 ± 1.27 ‰). Our study suggests that polymorphisms of XRCC1 gene may influence chromosomal damage in BD-exposed workers.
Department of Environmental Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Menglong
M
Xiang
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Lei
L
Sun
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Xiaomei
X
Dong
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Huan
H
Yang
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Wen-bin
WB
Liu
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Niya
N
Zhou
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Xue
X
Han
Department of Environmental Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Ziyuan
Z
Zhou
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Zhihong
Z
Cui
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Jing-yi
JY
Liu
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Jia
J
Cao
Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Lin
L
Ao
D002070:Butadienes / Q000378:metabolism / Q000633:toxicity*; D002681:China; D002869:Chromosome Aberrations / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D015254:DNA Modification Methylases / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D045643:DNA Repair Enzymes / Q000235:genetics; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests; D016273:Occupational Exposure; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D025521:Tumor Suppressor Proteins / Q000235:genetics; D014556:Urine / Q000737:chemistry; D000076105:X-ray Repair Cross Complementing Protein 1
D002070:Butadienes; D004268:DNA-Binding Proteins; D025521:Tumor Suppressor Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; D015254:DNA Modification Methylases; C515491:MGMT protein, human; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D045643:DNA Repair Enzymes; C031763:1,3-butadiene