Biogas production from Macrocystis pyrifera biomass in seawater system.
Marine sediments from littoral and sublittoral location were evaluated as inocula for methane production from anaerobic fermentation of Macrocystis pyrifera in seawater system. Littoral sediment showed the higher methanogenetic activity from acetate and resulted in a higher biomethane yield of 217.1±2.4mL/g-VS, which was comparable with that reported in freshwater system with desalted seaweeds. With 0.8mM sodium molybdate added, both the maximal methane yield and concentration increased while the lag-time was greatly shortened, suggesting that sulfate was one of the major inhibitors. Microbial community analysis revealed that degradation of M. pyrifera needed cooperation of very complex microbial populations. Hydrogenotrophic methanogens had an absolute dominance in distribution compared with the acetotrophic ones, indicating syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis might play important roles in the thalassic anaerobic fermentation system. These results clearly showed that biomethane production of raw seaweeds in seawater system was feasible.
Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 2661 01, China.
Xiaolei
X
Fan
Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 2661 01, China. Electronic address: guorb@qibebt.ac.cn.
Rongbo
R
Guo
Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 2661 01, China.
Xianzheng
X
Yuan
Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 2661 01, China.
Yanling
Y
Qiu
Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 2661 01, China.
Zhiman
Z
Yang
Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 2661 01, China; University of Chinese Academy of Science, Beijing 10049, China.
Fei
F
Wang
Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 2661 01, China; University of Chinese Academy of Science, Beijing 10049, China.
Mengting
M
Sun
College of Chemical Science and Engineering, Qingdao University, Qingdao 266071, China.
Xiaoxian
X
Zhao
D000085:Acetates / Q000378:metabolism; D001105:Archaea / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D056804:Biofuels / Q000382:microbiology; D018533:Biomass; D005285:Fermentation; D019015:Geologic Sediments; D044662:Macrocystis / Q000382:microbiology*; D008697:Methane / Q000096:biosynthesis*; D008982:Molybdenum; D012623:Seawater; D013431:Sulfates
D000085:Acetates; D056804:Biofuels; D013431:Sulfates; D008982:Molybdenum; C024687:sodium molybdate(VI); D008697:Methane
Anaerobic fermentation; Macrocystis pyrifera; Marine sediments; Methanogenetic pathway; Sulfate