Cytotoxic effect of inositol hexaphosphate and its Ni(II) complex on human acute leukemia Jurkat T cells.
Inositol hexaphosphate (InsP6) is present in cereals, legumes, nuts and seed oils and is biologically active against some tumor and cancer cells. Herein, this study aimed at evaluating the cellular toxicity, antiproliferative activity and effects on cell cycle progression of free InsP6 and InsP6-Ni(II) of leukemic T (Jurkat) and normal human cells. Treatments with InsP6 at concentrations between 1.0 and 4.0mM significantly decreased the viability of Jurkat cells, but showed no cytotoxic effect on normal human lymphocytes. Treatment with InsP6-Ni(II) complex at concentrations between 0.05 and 0.30 mM showed an anti-proliferative dose and a time-dependent effect, with significantly reduced cell viability of Jurkat cells but showed no cytotoxic effect on normal human lymphocytes as compared to the control. Ni(II) free ion was toxic to normal cells while InsP6-Ni(II) had no cytotoxic effect. The InsP6-Ni(II) complex potentiated (up to 10×) the antiproliferative effect of free InsP6 on Jurkat cells. The cytometric flow assay showed that InsP6 led to an accumulation of cells in the G0/G1 phase of the cell cycle, accompanied by a decrease in the number of cells in S and G2/M phases, whereas InsP6-Ni(II) has led to an accumulation of cells in the S and G2/M phases. Our findings showed that InsP6-Ni(II) potentiates cytotoxic effects of InsP6 on Jurkat cells and may be a potential adjuvant in the treatment of cancer.
Departamento de Química, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, Brazil.
Eliane May
EM
de Lima
Departamento de Biologia Celular, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, Brazil.
Carla Cristine
CC
Kanunfre
Departamento de Biologia Celular, Universidade Federal do Paraná, Rua Francisco H. dos Santos, s/n, 81531-990 Curitiba, Brazil.
Lucas Ferrari
LF
de Andrade
Departamento de Engenharia de Alimentos, Universidade Estadual de Ponta Grossa, Brazil. Av. Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, Brazil.
Daniel
D
Granato
Departamento de Química, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, Brazil. Electronic address: ndrosso@uepg.br.
Neiva Deliberali
ND
Rosso
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D006801:Humans; D019169:Jurkat Cells; D009532:Nickel / Q000737:chemistry*; D010833:Phytic Acid / Q000737:chemistry / Q000494:pharmacology*
D000970:Antineoplastic Agents; D010833:Phytic Acid; D009532:Nickel
Antitumoral agent; Inositol hexaphosphate; InsP(6)–Ni(II) complex; Jurkat cells