PCGEM1 stimulates proliferation of osteoarthritic synoviocytes by acting as a sponge for miR-770.
Long non-coding RNAs (lncRNAs) have been reported to play important roles in cellular metabolism and development. Various diseases have been associated with aberrant expression of lncRNAs and the related dysregulation of mRNAs. An lncRNA profiling assay was carried out to identify the key lncRNA in osteoarthritic human synoviocytes; the results revealed that prostate cancer gene expression marker 1 (PCGEM1) was significantly overexpressed in osteoarthritic synoviocytes. Exogenous overexpression of PCGEM1 inhibited apoptosis, induced autophagy, and stimulated the proliferation of human synoviocytes. The increased expression of PCGEM1 in human synoviocytes also suppressed the expression of miR-770. Transfection of the miR-770 precursor resulted in reduced proliferation, and induced apoptosis of human synoviocytes. This effect of miR-770 expression was reversed by co-introduction of PCGEM1. Target validation showed a direct binding between PCGEM1 and miR-770. We demonstrate that PCGEM1 act as sponge lncRNA for miR-770 that regulates proliferation/apoptosis and autophagy, and suggest PCGEM1 as possible target for OA therapy.
Department of Biological Sciences, College of Natural Sciences, Wonkwang University, Iksan, Chunbuk, 570-749, Korea.
Yeonho
Y
Kang
Department of Biological Sciences, College of Natural Sciences, Wonkwang University, Iksan, Chunbuk, 570-749, Korea.
Jinsoo
J
Song
Department of Biological Sciences, College of Natural Sciences, Wonkwang University, Iksan, Chunbuk, 570-749, Korea.
Dongkyun
D
Kim
Department of Biological Sciences, College of Natural Sciences, Wonkwang University, Iksan, Chunbuk, 570-749, Korea.
Chiyeon
C
Ahn
Department of Biological Sciences, College of Natural Sciences, Wonkwang University, Iksan, Chunbuk, 570-749, Korea.
Sujung
S
Park
Department of Orthopedic Surgery, Wonkwang University School of Medicine, Iksan, Chunbuk, 570-749, Korea.
Churl-Hong
CH
Chun
Department of Biological Sciences, College of Natural Sciences, Wonkwang University, Iksan, Chunbuk, 570-749, Korea.
Eun-Jung
EJ
Jin
D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D010003:Osteoarthritis / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000378:metabolism*; D013583:Synovial Membrane / Q000378:metabolism*
C000609533:MIRN-770 microRNA, human; D035683:MicroRNAs; C416084:PCGEM1 non-coding RNA, human; D062085:RNA, Long Noncoding
PCGEM1; apoptosis; autophagy; miR-770; osteoarthritis; proliferation; synoviocytes