Microbial community structure and function in response to the shift of sulfide/nitrate loading ratio during the denitrifying sulfide removal process.
Influence of acetate-C/NO3(-)-N/S(2-) ratio to the functional microbial community during the denitrifying sulfide removal process is poorly understood. Here, phylogenetic and functional bacterial community for elemental sulfur (S(0)) recovery and nitrate (NO3(-)) removal were investigated with the switched S(2-)/NO3(-) molar ratio ranged from 5/2 to 5/9. Optimized S(2-)/NO3(-) ratio was evaluated as 5/6, with the bacterial genera predominated with Thauera, Enterobacter, Thiobacillus and Stappia, and the sqr gene highly expressed. However, insufficient or high loading of acetate and NO3(-) resulted in the low S(0) recovery, and also significantly modified the bacterial community and genetic activity. With S(2-)/NO3(-) ratio of 5/2, autotrophic S(2-) oxidization genera were dominated and NO3(-) reduction activity was low, confirmed by the low expressed nirK gene. In contrast, S(2-)/NO3(-) ratio switched to 5/8 and 5/9 introduced diverse heterotrophic nitrate reduction and S(0) over oxidization genera in accompanied with the highly expressed nirK and sox genes.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China.
Cong
C
Huang
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China.
Zhi-Ling
ZL
Li
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China.
Fan
F
Chen
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China.
Qian
Q
Liu
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China.
You-Kang
YK
Zhao
Institute for Environmental Genomics, Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA; Earth Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94270, USA.
Ji-Zhong
JZ
Zhou
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China; Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China. Electronic address: waj0578@hit.edu.cn.
Ai-Jie
AJ
Wang
D052818:Autotrophic Processes; D001419:Bacteria / Q000145:classification / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D058440:Denitrification / Q000502:physiology; D052836:Heterotrophic Processes; D009566:Nitrates / Q000737:chemistry / Q000378:metabolism*; D009589:Nitrogen Oxides; D013440:Sulfides / Q000737:chemistry / Q000378:metabolism*; D013455:Sulfur / Q000737:chemistry
D009566:Nitrates; D009589:Nitrogen Oxides; D013440:Sulfides; D013455:Sulfur
Continuous stirred tank reactor (CSTR); Denitrifying sulfide removal process; Function; Microbial community structure; Sulfide/nitrate loading ratio