Heterogeneity of Genetic Damage in Cervical Nuclei and Lymphocytes in Women with Different Levels of Dysplasia and Cancer-Associated Risk Factors.
The comet assay can be used to assess genetic damage, but heterogeneity in the length of the tails is frequently observed. The aims of this study were to evaluate genetic damage and heterogeneity in the cervical nuclei and lymphocytes from patients with different levels of dysplasia and to determine the risk factors associated with the development of cervical cancer. The study included 97 females who presented with different levels of dysplasia. A comet assay was performed in peripheral blood lymphocytes and cervical epithelial cells. Significant genetic damage (P ≤ 0.05) was observed only in patients diagnosed with nuclei cervical from dysplasia III (NCDIII) and lymphocytes from dysplasia I (LDI). However, the standard deviations of the tail lengths in the cervical nuclei and lymphocytes from patients with dysplasia I were significantly different (P ≤ 0.0001) from the standard deviations of the tail lengths in the nuclei cervical and lymphocytes from patients with DII and DIII (NCDII, NCDIII and LDII, LDIII), indicating a high heterogeneity in tail length. Results suggest that genetic damage could be widely present but only manifested as increased tail length in certain cell populations. This heterogeneity could obscure the statistical significance of the genetic damage.
Cellular and Molecular Biology Department, Guadalajara University, Juarez 976, Colonia Centro, 44100 Guadalajara, JAL, Mexico.
Carlos
C
Alvarez-Moya
Cellular and Molecular Biology Department, Guadalajara University, Juarez 976, Colonia Centro, 44100 Guadalajara, JAL, Mexico.
Mónica
M
Reynoso-Silva
Unit of Medical and Pharmaceutical Biotechnology, CIATEJ, Avenida Normalistas 800, Colinas de la Normal, 44270 Guadalajara, JAL, Mexico.
Alejandro A
AA
Canales-Aguirre
Dysplasias Unit, Institute of Cancerology Jaliscience, General Coronado 515, Colonia Centro, 44100 Guadalajara, JAL, Mexico.
José O
JO
Chavez-Chavez
Mastitis and Molecular Diagnosis, Guadalajara University, Juarez 976, Colonia Centro, 44100 Guadalajara, JAL, Mexico.
Hugo
H
Castañeda-Vázquez
Cellular and Molecular Biology Department, Guadalajara University, Juarez 976, Colonia Centro, 44100 Guadalajara, JAL, Mexico.
Alfredo I
AI
Feria-Velasco
D002467:Cell Nucleus / Q000235:genetics* / Q000473:pathology; D004249:DNA Damage / Q000235:genetics; D005260:Female; D018740:Genetic Heterogeneity; D006801:Humans; D008214:Lymphocytes / Q000473:pathology*; D009363:Neoplasm Proteins / Q000235:genetics; D012307:Risk Factors; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
D009363:Neoplasm Proteins; C040044:neoplasm-associated factor