Utilization of fish meal and fish oil for production of Cryptococcus sp. MTCC 5455 lipase and hydrolysis of polyurethane thereof.
Fish meal has been used as an additional nitrogen source and fish oil as inducer for the growth and production of lipase from Cryptococcus sp. MTCC 5455. A response surface design illustrated that the optimum factors influencing lipase production were fish meal, 1.5 %, w/v, Na2HPO4, 0.2 %, w/v, yeast extract, 0.25 %, w/v and sardine oil, 2.0 %, w/v with an activity of 71.23 U/mL at 96 h and 25 °C, which was 48.39 % higher than the conventional one-factor-at-a-time method. The crude concentrated enzyme hydrolyzed polyurethane (PUR) efficiently and hydrolysis was 94 % at 30 °C and 96 h. The products, diethylene glycol and adipic acid were quantified by HPLC and scanning electron microscopic studies of the degraded polymer showed significant increase in size of the holes from 24 to 72 h of incubation. Hydrolysis of PUR within 96 h makes the lipase novel for disposal of PUR and provides an innovative solution to the problems created by plastic wastes.
Department of Biomedical Engineering, Sathyabama University, Sholinganallur, Chennai, 600 119 India.
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Thirunavukarasu
Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai, 600 020 India.
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Purushothaman
Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai, 600 020 India.
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Gowthaman
Bioindustrial Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572 Japan.
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Nakajima-Kambe
Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai, 600 020 India.
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Rose
Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai, 600 020 India.
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Kamini
Cryptococcus sp.; Fish meal; Lipase; Polyurethane degradation; Sardine oil