Bacteria Present in Comadia redtenbacheri Larvae (Lepidoptera: Cossidae).
The external and internal culturable bacterial community present in the larvae of Comadia redtenbacheri Hammerschmidt, an edible insect, was studied. Characterization of the isolates determined the existence of 18 morphotypes and phylogenetic analysis of the 16S rRNA gene revealed the existence of Paenibacillus sp., Bacillus safensis, Pseudomonas sp., Bacillus pseudomycoides, Corynebacterium variabile, Enterococcus sp., Gordonia sp., Acinetobacter calcoaceticus, Arthrobacter sp., Micrococcus sp., and Bacillus cereus. Greater diversity of bacteria was found in those larvae obtained from vendors than in those directly taken from Agave plants in nature. Many of the larvae obtained from vendors presented signs of potential disease, and after the analysis, results showed a greater bacterial community compared with the larvae with a healthy appearance. This indicates that bacterial flora can vary in accordance with how the larvae are handled during extraction, collection, and transport.
Colegio de Postgraduados. Campus Montecillo. Km 36.5 Carretera México-Texcoco. Montecillo, Estado de México, México.
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Hernández-Flores
Colegio de Postgraduados. Campus Montecillo. Km 36.5 Carretera México-Texcoco. Montecillo, Estado de México, México. llcelina@colpos.mx.
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Llanderal-Cázares
Colegio de Postgraduados. Campus Montecillo. Km 36.5 Carretera México-Texcoco. Montecillo, Estado de México, México.
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Guzmán-Franco
Colegio de Postgraduados. Campus Montecillo. Km 36.5 Carretera México-Texcoco. Montecillo, Estado de México, México.
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Aranda-Ocampo
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D007814:Larva / Q000254:growth & development / Q000382:microbiology; D064307:Microbiota; D008969:Molecular Sequence Data; D009036:Moths / Q000254:growth & development / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
agave; appearance; bacterial community; collection procedure; entomophagy