Novel 3D-microcarriers from recombinant spidroin for regenerative medicine.
Microcarriers generated from recombinant spidroin 1F9 are suitable for use as an injection material. The microcarriers were a heterogeneous mixture of microgel particles ranging from 50 to 300 µm in size with the predominance of particles of 50-150 µm. The surface of these microparticles had a complex topography and ensured efficient cultivation of primary and immortalized fibroblasts. Intradermal injections of microgel suspensions into the area of full-thickness skin wounds did not lead to the development of acute inflammation in mice; instead, they accelerated the recovery of skin tissue and stimulated neurogenesis and angiogenesis.
Moscow State University, Moscow, 119991, Russia, mmoisenovich@mail.ru.
M M
MM
Moisenovich
N V
NV
Malyuchenko
A Y
AY
Arkhipova
M S
MS
Kotlyarova
L I
LI
Davydova
A V
AV
Goncharenko
O I
OI
Agapova
M S
MS
Drutskaya
V G
VG
Bogush
I I
II
Agapov
V G
VG
Debabov
M P
MP
Kirpichnikov
D016475:3T3 Cells; D000818:Animals; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D005349:Fibroins / Q000737:chemistry*; D051379:Mice; D008863:Microspheres; D010316:Particle Size; D011994:Recombinant Proteins / Q000737:chemistry*; D044968:Regenerative Medicine / Q000379:methods*; D012879:Skin Physiological Phenomena; D014945:Wound Healing / Q000187:drug effects
D004337:Drug Carriers; D011994:Recombinant Proteins; D005349:Fibroins