Poly(N-isopropylacrylamide)-gated Fe3O4/SiO2 core shell nanoparticles with expanded mesoporous structures for the temperature triggered release of lysozyme.
Core-shell nanoparticles comprised of Fe3O4 cores and a mesoporous silica shell with an average expanded pore size of 6.07 nm and coated with a poly(N-isopropylacrylamide) (PNIPAM) layer (CS-MSNs-EP-PNIPAM) were prepared and characterized. The nanoparticles was loaded with (Ru(bipy)3(2+)) dye or an antibacterial enzyme, lysozyme, to obtain CS-MSNs-EP-PNIPAM-Ru(bipy)3(2+) and CS-MSNs-EP-PNIPAM-Lys, respectively. The lysozyme loading was determined to be 160 mg/g of nanoparticle. It was seen that Ru(bipy)3(2+) and lysozyme release was minimal at a room temperature of 25 °C while at physiological temperature (37 °C), abrupt release was observed. The applicability of the CS-MSNs-EP-PNIPAM-Lys was further tested with two Gram-positive bacteria samples, Bacillus cereus and Micrococcus luteus. At physiological temperature, the nanoparticles were shown to reduce bacterial growth, indicating a successful release of lysozyme from the nanoparticles. This nanoparticle system shows potential as a nanocarrier for the loading of similarly sized proteins or other species as a drug delivery platform.
Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universidad Politécnica de Valencia, Universidad de Valencia, Departamento de Química, Universidad Politécnica de Valencia, 46022 Valencia, Spain; Department of Chemical Engineering and Materials Science, University of California Davis, 95616 Davis, California, United States.
Erick
E
Yu
Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universidad Politécnica de Valencia, Universidad de Valencia, Departamento de Química, Universidad Politécnica de Valencia, 46022 Valencia, Spain.
Irene
I
Galiana
Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universidad Politécnica de Valencia, Universidad de Valencia, Departamento de Química, Universidad Politécnica de Valencia, 46022 Valencia, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). Electronic address: rmaez@qim.upv.es.
Ramón
R
Martínez-Máñez
Department of Chemical Engineering and Materials Science, University of California Davis, 95616 Davis, California, United States. Electronic address: pstroeve@ucdavis.edu.
Pieter
P
Stroeve
Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universidad Politécnica de Valencia, Universidad de Valencia, Departamento de Química, Universidad Politécnica de Valencia, 46022 Valencia, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN).
María D
MD
Marcos
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN).
Elena
E
Aznar
Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universidad Politécnica de Valencia, Universidad de Valencia, Departamento de Química, Universidad Politécnica de Valencia, 46022 Valencia, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN).
Félix
F
Sancenón
Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universidad Politécnica de Valencia, Universidad de Valencia, Departamento de Química, Universidad Politécnica de Valencia, 46022 Valencia, Spain.
José R
JR
Murguía
Institut de Ciència dels Materials (ICMUV), Universitat de València, P.P Box 2085, 46071 Valencia, Spain.
Pedro
P
Amorós
D000180:Acrylic Resins / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry*; D001409:Bacillus cereus / Q000187:drug effects; D005296:Ferrous Compounds / Q000737:chemistry*; D016982:Micrococcus luteus / Q000187:drug effects; D009113:Muramidase / Q000008:administration & dosage / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013696:Temperature
D000180:Acrylic Resins; D000900:Anti-Bacterial Agents; D005296:Ferrous Compounds; C052970:poly-N-isopropylacrylamide; C020748:ferrous sulfate; D012822:Silicon Dioxide; D009113:Muramidase
Mesoporous silica; PNIPAM; Pore expansion; Protein delivery; Triggered release