Development of EBV-encoded small RNA targeted PCR to classify EBV positive diffuse large B-cell lymphoma (DLBCL) of the elderly.
Epstein-Barr virus (EBV)-positive diffuse large B-cell lymphoma (DLBCL) of the elderly has been included in the 2008 WHO classification of lymphoma as a new provisional entity. EBV-positive DLBCL of the elderly is newly classified due to the main occurrence usually in patients of older than 50-year-old. This study was performed in 91 DLBCL patients from January 2002 to December 2012 in Catholic university of St. Vincent Hospital. Age distribution of the patients was 14~87-year-old. Specimens were collected from lymph nodes (n = 45) and extra-lymph nodes (n = 46). EBV encoded small RNA1 in situ hybridization (EBER1-ISH) known as a standard method for the diagnosis of DLBCL. In this study, nested PCR of DNA polymerase gene and EBER PCR were conducted to detect EBV. Presence of EBV was indicated in 3 samples (3.30%) by EBER-ISH, 26 samples (28.57%) by nPCR, and 3 samples (3.30%) by EBER PCR. The concordant results were obtained from EBER1-ISH and EBER PCR. Two samples were classified as EBV-positive DLBCL of the elderly among 91 DLBCL patients. Previously, the incidence rate of DLBCL of the elderly in Asia has been reported as 5~11%, but the result in this study showed a slightly lower incidence rate. To our knowledge, this is the first report on EBV-positive DLBCL of the elderly in Suwon area, Korea. EBER1-ISH and EBER PCR developed in this study may be helpful in classification of EBV-positive DLBCL of the elderly in future.
Department of Hospital Pathology, St. Vincent's Hospital, The Catholic University of Korea Seoul 16247, Korea.
Jaewang
J
Lee
Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University Wonju 26493, Korea.
Min
M
Park
Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University Wonju 26493, Korea.
Min Ho
MH
Lee
Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University Wonju 26493, Korea.
Hyun Jun
HJ
Woo
Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University Wonju 26493, Korea.
Hyun-Woo
HW
Kim
Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University Wonju 26493, Korea.
Ji Yeong
JY
Yang
Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University Chungnam, Asan 31538, Korea.
Yong-Bin
YB
Eom
Department of Clinical Laboratory Science, Semyung University Jaecheon 27136, Korea.
Sa-Hyun
SH
Kim
Department of Hospital Pathology, St. Vincent's Hospital, The Catholic University of Korea Seoul 16247, Korea.
Changyoung
C
Yoo
Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University Wonju 26493, Korea.
Jong-Bae
JB
Kim
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D020031:Epstein-Barr Virus Infections / Q000473:pathology / Q000821:virology*; D005260:Female; D004854:Herpesvirus 4, Human / Q000145:classification* / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000235:genetics; D055815:Young Adult
D012367:RNA, Viral
EBV-encoded small RNA; Epstein-Barr virus; diffuse large B-cell lymphoma