Cloning and transformation analysis of isoflavone synthase gene into Minshan Trifolium pratense.
The aim of this study was to clone the isoflavone synthase (IFS) gene and establish the recombinant Minshan Trifolium pratense. The IFS gene was cloned from the callus of Minshan T. pratense using reverse transcription-polymerase chain reaction. The plant expression vector pRI101-AN-IFS was constructed and introduced into Agrobacterium tumefaciens strain LBA4404, and then screened under cephalosporin. IFS expression was detected by reverse transcription-polymerase chain reaction. The IFS gene was cloned successfully. Sequence analysis indicated that IFS gene had high homology with similar genes from other plants. The IFS-overexpressing callus was obtained by introducing the LBA4404-harboring IFS-pRI101-AN-IFS vector into T. pratense calluses.
College of Life Science and Technology, Sanquan School, Xinxiang Medical University, Xinxiang, China.
H H
HH
Hu
College of Life Science and Technology, Xinxiang Medical University, Xinxiang, China.
C Q
CQ
Jing
College of Life Science and Technology, Sanquan School, Xinxiang Medical University, Xinxiang, China.
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R
Liu
College of Life Science and Technology, Sanquan School, Xinxiang Medical University, Xinxiang, China.
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WD
Li
College of Life Science and Technology, Xinxiang Medical University, Xinxiang, China fenghuigen@126.com.
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HG
Feng
D003001:Cloning, Molecular; D010105:Oxygenases / Q000235:genetics*; D030821:Plants, Genetically Modified; D014170:Transformation, Genetic; D029921:Trifolium / Q000235:genetics* / Q000378:metabolism
D010105:Oxygenases; C048489:isoflavone synthase