Surface modification of electrospun poly(methyl methacrylate) (PMMA) nanofibers for the development of in vitro respiratory epithelium model.
Scaffold design is an important aspect of in vitro model development. In this study, nanoscaffold surface modification, namely UV radiation and genipin cross-linking to immobilize collagen on the surface of electrospun poly (methyl methacrylate) (PMMA) nanofiber sheet was investigated. Samples were divided into four groups; PMMA nanofibers (PMMA), collagen-coated PMMA nanofibers (PMMACOL), genipin cross-linked collagen-coated PMMA nanofibers (PMMAGEN), and UV-irradiated collagen-coated PMMA nanofibers (PMMAUV). 6 h of UV radiation significantly reduced the hydrophobicity of PMMA nanofibers from (131.88° ± 1.33°) to (110.04° ± 0.27°) (p < 0.05). The amount of collagen immobilized was significantly higher in PMMAGEN group (239.36 ± 16.63 μg collagen/mg nanofibers) (p < 0.05) compared to the other groups. RECs on all scaffold expressed epithelial cell-specific markers (CK18 and CK14), mucin-producing cell marker (MUC5Ac) and were actively proliferating, based on the positive expression of Ki67. Total number of attached cells was significantly the highest in PMMAUV group on day 9 (6.44 × 10(4) ± 2.77 × 10(4) cells/cm(2)) and it has the highest proliferation rate from day 4 to 9 (0.005 ± 0.003 h(-1)) compared to the other groups. Even though PMMAGEN group showed the highest collagen adsorption, in terms of cells attachment and proliferation, PMMAUV group showed a better outcome compared to the other groups. Thus, PMMAUV scaffold is more suitable to be used in the construction of in vitro respiratory epithelial model.
a Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre , Universiti Kebangsaan Malaysia , Kuala Lumpur , Malaysia.
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AR
Rabiatul
a Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre , Universiti Kebangsaan Malaysia , Kuala Lumpur , Malaysia.
Y
Y
Lokanathan
a Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre , Universiti Kebangsaan Malaysia , Kuala Lumpur , Malaysia.
C M
CM
Rohaina
a Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre , Universiti Kebangsaan Malaysia , Kuala Lumpur , Malaysia.
S R
SR
Chowdhury
b Ear, Nose and Throat Consultant Clinic, Ampang Puteri Specialist Hospital , Ampang , Selangor , Malaysia.
B S
BS
Aminuddin
a Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre , Universiti Kebangsaan Malaysia , Kuala Lumpur , Malaysia.|c Faculty of Medicine, Department of Physiology , Universiti Kebangsaan Malaysia , Kuala Lumpur , Malaysia.
B H I
BH
Ruszymah
D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D024042:Collagen Type I / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D020545:Respiratory Mucosa / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism
D001672:Biocompatible Materials; D015415:Biomarkers; D024042:Collagen Type I; D055767:Immobilized Proteins; D019904:Polymethyl Methacrylate
UV radiation; airway epithelium; collagen type 1; genipin cross-linking; tissue engineering