Targeting essential Eimeria ninakohlyakimovae sporozoite ligands for caprine host endothelial cell invasion with a phage display peptide library.
Eimeria ninakohlyakimovae is an important coccidian parasite of goats which causes severe diarrhoea in young animals. Specific molecules that mediate E. ninakohlyakimovae host interactions and molecular mechanisms involved in the pathogenesis are still unknown. Although strong circumstantial evidence indicates that E. ninakohlyakimovae sporozoite interactions with caprine endothelial host cells (ECs) are specific, hardly any information is available about the interacting molecules that confer host cell specificity. In this study, we describe a novel method to identify surface proteins of caprine umbilical vein endothelial cells (CUVEC) using a phage display library. After several panning rounds, we identified a number of peptides that specifically bind to the surface of CUVEC. Importantly, caprine endothelial cell peptide 2 (PCEC2) and PCEC5 selectively reduced the infection rate by E. ninakohlyakimovae sporozoites. These preliminary data give new insight for the molecular identification of ligands involved in the interaction between E. ninakohlyakimovae sporozoites and host ECs. Further studies using this phage approach might be useful to identify new potential target molecules for the development of anti-coccidial drugs or even new vaccine strategies.
Parasitology Unit, Department of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, 35416, Arucas, Las Palmas, Spain. antonio.ruiz@ulpgc.es.
A
A
Ruiz
Parasitology Unit, Department of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, 35416, Arucas, Las Palmas, Spain.
D
D
Pérez
Parasitology Unit, Department of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, 35416, Arucas, Las Palmas, Spain.
M C
MC
Muñoz
Parasitology Unit, Department of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, 35416, Arucas, Las Palmas, Spain.
J M
JM
Molina
Institute of Parasitology, Faculty of Veterinary Medicine, Justus Liebig University Giessen, Giessen, Germany.
A
A
Taubert
The W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.
M
M
Jacobs-Lorena
The W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.
J
J
Vega-Rodríguez
Parasitology Unit, Department of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, 35416, Arucas, Las Palmas, Spain.
A M
AM
López
Institute of Parasitology, Faculty of Veterinary Medicine, Justus Liebig University Giessen, Giessen, Germany.
C
C
Hermosilla
D000818:Animals; D003048:Coccidiosis / Q000235:genetics / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D004539:Eimeria / Q000254:growth & development / Q000502:physiology*; D042783:Endothelial Cells / Q000276:immunology* / Q000469:parasitology; D015511:Goat Diseases / Q000235:genetics / Q000276:immunology* / Q000469:parasitology; D006041:Goats; D006790:Host-Parasite Interactions; D008024:Ligands; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000276:immunology*; D034101:Sporozoites / Q000254:growth & development / Q000502:physiology
D008024:Ligands; D019151:Peptide Library; D010455:Peptides
Coccidiosis; Eimeria ninakohlyakimovae; Endothelial cells; Goats; Phage display