Assessment of a Protease Inhibitor Peptide for Anti-Ageing.
Ageing and skin exposure to UV radiation induces production and activation of matrix metalloproteinases (MMPs) and human neutrophil elastase (HNE). These enzymes are known to break down the extracellular matrix (ECM) which leads to wrinkle formation. Here, we demonstrated the potential of a solid-in-oil nanodispersion containing a competitive inhibitor peptide of HNE mixed with hyaluronic acid (HA), displaying 158 nm of mean diameter, to protect the skin against the ageing effects. Western blot analysis demonstrated that activation of MMP-1 in fibroblasts by HNE treatment is inhibited by the solid-in-oil nanodispersion containing the peptide and HA. The results clearly demonstrate that solid-in-oil nanodispersion containing the HNE inhibitor peptide is a promising strategy for anti-ageing effects. This effect can be seen particularly by ECM regulation by affecting fibroblasts. The formulation also enhances the formation of thicker bundles of actin filaments.
Madalena
M
Martins
Nuno G
NG
Azoia
Ana C
AC
Carvalho
Carla
C
Silva
Teresa
T
Matamá
Andreia C
AC
Gomes
Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal. artur@deb.uminho.pt.
Artur
A
Cavaco-Paulo
D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors*; D010455:Peptides; D011480:Protease Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000187:drug effects; D015595:Skin Aging / Q000187:drug effects*; D013552:Swine
D010455:Peptides; D011480:Protease Inhibitors; D006820:Hyaluronic Acid; D010196:Pancreatic Elastase