A novel antagonistic role of natural compound icariin on neurotoxicity of amyloid β peptide.
OBJECTIVE
Amyloid β-peptide (Aβ) has been shown to be responsible for senile plaque formation and cell damage in Alzheimer's disease (AD). This study was aimed to explore the role of natural compound icariin on the aggregation and the cytotoxicity of Aβ in vitro.


METHODS
Thioflavin T (ThT) fluorescence assay and transmission electron microscopy (TEM) imaging were done to determine the influence of icariin on the aggregation of Aβ1-42 peptide. MTT assay was used to evaluate the protective effect of icariin on Aβ1-42 induced cytotoxicity in neuroblastoma SH-SY5Y cells.


RESULTS
Icariin inhibited Aβ1-42 aggregation in a dose-dependent manner. Additionally, icariin also prevented the cytotoxicity of Aβ1-42 in SH-SY5Y cells by decreasing the production of peroxide hydrogen during the aggregation of this peptide.


CONCLUSIONS
The results indicated a novel antagonistic role of icariin in the neurotoxicity of Aβ1-42 via inhibiting its aggregation, suggesting that icariin might have potential therapeutic benefits to delay or modify the progression of AD.
College of Medicine & Bioengineering, Chongqing Technology of University, Chongqing ; Chongqing Key Lab of Catalysis & Functional Organic Molecules, Chongqing Technology & Business University, Chongqing, PR China.
Jianhui
J
Liu
Zixuan
Z
Liu
Yonglan
Y
Zhang
College of Medicine & Bioengineering, Chongqing Technology of University, Chongqing ; Chongqing Key Lab of Catalysis & Functional Organic Molecules, Chongqing Technology & Business University, Chongqing, PR China.
Fei
F
Yin
D000544:Alzheimer Disease / Q000188:drug therapy* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D052160:Benzothiazoles; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005419:Flavonoids / Q000008:administration & dosage*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure; D010446:Peptide Fragments / Q000187:drug effects; D066263:Protein Aggregation, Pathological / Q000188:drug therapy* / Q000473:pathology; D013844:Thiazoles / Q000737:chemistry
D016229:Amyloid beta-Peptides; D000975:Antioxidants; D052160:Benzothiazoles; D005419:Flavonoids; D010446:Peptide Fragments; D013844:Thiazoles; C009462:thioflavin T; D006861:Hydrogen Peroxide; C056599:icariin