Relationship between the single nucleotide polymorphisms of β₂-adrenergic receptor 5'-regulatory region and essential hypertension in Chinese Kazakh ethnic minority group.
OBJECTIVE
To study the correlation of β2-AR gene 5'-regulatory region SNPs and essential hypertension (EH) in Chinese Kazakh ethnic minority group.


METHODS
The Sequenom MassArray(®) SNP detection technology was used to detect β2-AR gene 5'-regulatory region SNPs in 150 Xinjiang Kazakh EH patients and 150 controls. Biochemical analyzer was used to detect lipid and other related biochemical parameters. SHEsis and other software were used to analyze linkage disequilibrium and haplotype.


RESULTS
Six loci rs205304 (-1023G/A), rs17108803 (-893T/G), rs12654778 (-654G/A), rs11168070 (-468C/G), rs11959427 (-367C/T) and rs2895795 (-1429T/A) polymorphisms of β2-AR gene 5'-regulatory region were found in the Xinjiang Kazakh populations. While, there was no significant difference between EH group and NH in genotypes and allele frequency of rs2053044, rs12654778, rs2895795, rs17108803 and rs11959427 (P>0.05). However; significant differences were detected of rs11168070 genotypes and allele frequency in two groups (P<0.05). Analysis of the linkage disequilibrium and haplotype in Kazakh population, there is a strong linkage disequilibrium of rs11168070, rs2053044, rs2895795 gene polymorphism in the EH group, and rs11168070, rs12654778, rs17108803 gene polymorphism in controls. Frequency of haplotype GTCCAT, GACTGT and ATGCGT in EH group was higher (P<0.05), while frequency of ATCTGT, ATGTGT, GTCCGT, GTCTAT, GACCAT and GTCTGT in the EH group was significantly lower than the control (P<0.05).


CONCLUSIONS
β2-AR gene 5'-regulatory region of rs11168070, rs2053044, rs17108803, rs12654778, rs11959427 and rs2895795 genetic polymorphism exists in Kazakh. Among them, rs11168070 locus genotype and allele frequency distribution in the two groups are significant differences. In six polymorphic loci, there is a strong linkage disequilibrium, which haplotypes GTCCAT, GACTGT, ATGCGT are risk factors of EH, and the ATCTGT, ATGTGT, GTCCGT, GTCTAT, GACCAT, GTCTGT are protective factors.
Department of Clinical Laboratory, The First Affiliated Hospital, Shihezi University School of Medicine Shihezi 832002, Xinjiang, P. R. China.
Weijuan
W
Cai
Department of Endocrinology and Metabolism, The First Affiliated Hospital, Shihezi University School of Medicine Shihezi 832002, Xinjiang, P. R. China.
Liang
L
Yin
Department of Emergency, Burqin County People's Hospital Burqin 836600, Xinjiang, P. R. China.
Jiang
J
Cheng
Department of Clinical Laboratory, The First Affiliated Hospital, Shihezi University School of Medicine Shihezi 832002, Xinjiang, P. R. China.
Shaoliang
S
Wang
Department of Clinical Laboratory, The First Affiliated Hospital, Shihezi University School of Medicine Shihezi 832002, Xinjiang, P. R. China.
Yu
Y
Wei
Department of Clinical Laboratory, The First Affiliated Hospital, Shihezi University School of Medicine Shihezi 832002, Xinjiang, P. R. China.
Wenjiang
W
Cao
Department of Clinical Laboratory, The First Affiliated Hospital, Shihezi University School of Medicine Shihezi 832002, Xinjiang, P. R. China.
Jiang
J
Cheng
D020121:5' Untranslated Regions; D000328:Adult; D044466:Asian People / Q000235:genetics*; D001794:Blood Pressure / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D006973:Hypertension / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics* / Q000503:physiopathology; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D008913:Minority Groups; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D065840:Protective Factors; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics*; D012307:Risk Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020121:5' Untranslated Regions; C553443:ADRB2 protein, human; D018343:Receptors, Adrenergic, beta-2
Kazakh; gene polymorphism; hypertension; β2-adrenergic receptor