miR-122-5p as a plasma biomarker of liver injury in fish exposed to microcystin-LR.
Recent studies have shown the presence of large amounts of microRNAs (miRNAs; miRs) from damaged cells in the peripheral blood. In this study, we investigated the levels of miRNAs circulating in the blood plasma of whitefish (Coregonus lavaretus) after exposure to microcystin-LR. We used real-time PCR to examine the relative expression of plasma levels of 4 miRNAs (miR-122-5p and let-7c-5p, the liver-enriched microRNAs, miR-148a-3p which promotes the hapatospecific phenotype in mammals, and miR-92a-3p, a cell proliferation and angiogenesis promoter, potentially hepatocarcinogenic) during the first 48 h after exposure to MC-LR. We observed a rapid increase of miR-122-5p levels 8 h after exposure (P < 0.05), which continued to the end of the experiment. Our results demonstrated that the plasma miR-122-5p was indicative of MC-LR-induced liver injury, exhibiting areas under the curve close to 1 in ROC analysis (AUC = 0.976, P < 0.001). Although plasma levels of miR-148a-3p and miR-92a-3p were significantly elevated by the end of the experiment, their discriminative power was lower than reported for the miR-122-5p. Based on these results and reports on miRNA-based diagnosis of liver injuries in mammals, plasma miR-122-5p could be considered as a robust, new generation diagnostic biomarker in fish, helpful for the non-invasive diagnosis of liver damage.
Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
M
M
Florczyk
Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
P
P
Brzuzan
Department of Salmonid Research in Rutki, Inland Fisheries Institute in Olsztyn, Olsztyn, Poland.
J
J
Krom
Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
M
M
Woźny
Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
A
A
Łakomiak
D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D015415:Biomarkers / Q000097:blood*; D008099:Liver / Q000187:drug effects* / Q000293:injuries / Q000473:pathology; D008387:Marine Toxins; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D052998:Microcystins / Q000633:toxicity*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012487:Salmonidae / Q000097:blood / Q000293:injuries / Q000378:metabolism*
D001427:Bacterial Toxins; D015415:Biomarkers; D008387:Marine Toxins; D035683:MicroRNAs; D052998:Microcystins; C057862:cyanoginosin LR
circulating microRNA; fish; hepatotoxicity; microcystin-LR