A functional proteomics approach to the comprehension of sarcoidosis.
Pulmonary sarcoidosis (Sar) is an idiopathic disease histologically typified by non-caseating epitheliod cell sarcoid granulomas. A cohort of 37 Sar patients with chronic persistent pulmonary disease was described in this study. BAL protein profiles from 9 of these Sar patients were compared with those from 8 smoker (SC) and 10 no-smoker controls (NSC) by proteomic approach. Principal Component Analysis was performed to clusterize the samples in the corresponding conditions highlighting a differential pattern profiles primarily in Sar than SC. Spot identification reveals thirty-four unique proteins involved in lipid, mineral, and vitamin Dmetabolism, and immuneregulation of macrophage function. Enrichment analysis has been elaborated by MetaCore, revealing 14-3-3ε, α1-antitrypsin, GSTP1, and ApoA1 as "central hubs". Process Network as well as Pathway Maps underline proteins involved in immune response and inflammation induced by complement system, innate inflammatory response and IL-6signalling. Disease Biomarker Network highlights Tuberculosis and COPD as pathologies that share biomarkers with sarcoidosis. In conclusion, Sar protein expression profile seems more similar to that of NSC than SC, conversely to other ILDs. Moreover, Disease Biomarker Network revealed several common features between Sar and TB, exhorting to orientate the future proteomics investigations also in comparative BALF analysis of Sar and TB.
Functional Proteomic Section, Department of Life Sciences, University of Siena, Siena,Italy. Electronic address: landi35@unisi.it.
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Landi
UOC Respiratory Diseases and Lung Transplantation, Department Internal and Specialist Medicine,University of Siena, Siena,Italy.
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Bargagli
UOC Respiratory Diseases and Lung Transplantation, Department Internal and Specialist Medicine,University of Siena, Siena,Italy.
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Carleo
Functional Proteomic Section, Department of Life Sciences, University of Siena, Siena,Italy.
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Bianchi
Functional Proteomic Section, Department of Life Sciences, University of Siena, Siena,Italy.
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Gagliardi
UOC Respiratory Diseases and Lung Transplantation, Department Internal and Specialist Medicine,University of Siena, Siena,Italy.
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Cillis
UOC Respiratory Diseases and Lung Transplantation, Department Internal and Specialist Medicine,University of Siena, Siena,Italy.
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Perari
UOC Respiratory Diseases and Lung Transplantation, Department Internal and Specialist Medicine,University of Siena, Siena,Italy.
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Refini
Department of Pneumology, Medical School Hannover, Carl-Neubergstrasse 1, 30625 Hannover, Germany.
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Prasse
Functional Proteomic Section, Department of Life Sciences, University of Siena, Siena,Italy.
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Bini
UOC Respiratory Diseases and Lung Transplantation, Department Internal and Specialist Medicine,University of Siena, Siena,Italy.
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Rottoli
D001992:Bronchoalveolar Lavage Fluid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D017565:Sarcoidosis, Pulmonary / Q000150:complications / Q000175:diagnosis* / Q000378:metabolism*; D012680:Sensitivity and Specificity; D015398:Signal Transduction; D012907:Smoking / Q000378:metabolism*; D014376:Tuberculosis / Q000378:metabolism*
D020543:Proteome
Bronchoalveolar lavage; Functional proteomics; Mass spectrometry; Pathway analysis; Sarcoidosis; Tuberculosis