Does dexamethasone act in neuropeptides SP and CGRP in neurogenic inflammation of the skin? An experimental study.
OBJECTIVE
To investigate the neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP) after subcutaneous injection of dexamethasone prior to skin incision in rats.


METHODS
Twenty seven Wistar-EPM-1 rats were randomly divided into three groups. The sham group (SG) of rats was injected with 0.9 % saline. The second group (Dexa) was injected with 1.0 mg/kg dexamethasone, and the third group (Dexa+) was injected with 10.0 mg/kg dexamethasone. In all groups, the three subcutaneous injections were performed 30 minutes prior to the surgical skin incision and tissue collection. SP and CGRP (15 kDa pro-CGRP and 5 kDa CGRP) were quantified by Western Blotting.


RESULTS
No statistically significant differences (p>0.05) were found in pro-CGRP, CGRP and SP values in all three groups.


CONCLUSIONS
The anti-inflammatory effect of dexamethasone did not occur when the substance P and calcitonin gene-related peptide levels were altered during the neurogenic inflammation process of skin wound healing in rats.
Universidade Federal de São Paulo, BR.
Jose Octavio Gonçalves de
JO
Freitas
UNIFESP, São Paulo, SP, BR.
Paulo Rogério
PR
Quieregatto
UNIFESP, São Paulo, SP, BR.
Bernardo
B
Hochman
UNIFESP, São Paulo, SP, BR.
Guilherme Abbud Franco
GA
Lapin
UNIFESP, BR.
Stella Maria Botequio
SM
Mella
Department of Neurology, School of Medicine, Universidade de São Paulo, BR.
Jessica Ruivo
JR
Maximino
Neuroregeneration Center, School of Medicine, USP, São Paulo, SP, BR.
Gerson
G
Chadi
Department of Surgery, UNIFESP, São Paulo, SP, BR.
Lydia Masako
LM
Ferreira
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015153:Blotting, Western; D015740:Calcitonin Gene-Related Peptide / Q000187:drug effects* / Q000378:metabolism; D003872:Dermatitis / Q000188:drug therapy* / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D007279:Injections, Subcutaneous; D008297:Male; D020078:Neurogenic Inflammation / Q000188:drug therapy* / Q000378:metabolism; D011897:Random Allocation; D017208:Rats, Wistar; D013373:Substance P / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
D000893:Anti-Inflammatory Agents; D013373:Substance P; D003907:Dexamethasone; D015740:Calcitonin Gene-Related Peptide