Handmade Cloned Buffalo (Bubalus bubalis) Embryos Produced from Somatic Cells Isolated from Milk and Ear Skin Differ in Their Developmental Competence, Epigenetic Status, and Gene Expression.
We compared the cloning efficiency of buffalo embryos produced by handmade cloning (HMC) using ear skin- and milk-derived donor cells. The blastocyst rate was lower (p < 0.05) for milk-derived than that for skin-derived embryos, whereas the total cell number and apoptotic index were similar. The global level of H3K9ac was higher (p < 0.05) in skin- than in milk-derived cells, whereas the level of H3K27me3 was similar in the two groups. The global level of H3K9ac was similar between milk-derived and in vitro-fertilized (IVF) blastocysts, which was higher (p < 0.05) than that in skin-derived blastocysts. The level of H3K27me3 was similar among the three groups. The expression level of IGF-1R and G6PD was higher (p < 0.05) in skin- than in milk-derived cells, whereas DNMT1, DNMT3a, and HDAC1 expression level was similar. In the blastocysts, the expression level of DNMT1, HDAC1, OCT4, and CDX2 was higher (p < 0.05) in skin-derived than that in IVF blastocysts. The expression level of DNMT3a and IGF-1R, was in the order (p < 0.05) skin-derived and IVF > milk-derived blastocysts and that of NANOG was (p < 0.05) IVF-> milk-derived > skin-derived blastocysts. The expression level of all these genes, except NANOG, was lower (p < 0.05) in milk- than in skin-derived or IVF blastocysts. In conclusion, milk-derived cells can be used for producing HMC embryos of quality similar to that of skin-derived embryos, although with a lower blastocyst rate.
1 Animal Biotechnology Section, Central Sheep and Wool Research Institute , Avikanagar-304501 (Rajasthan), India .
Basanti
B
Jyotsana
2 Animal Biotechnology Centre, National Dairy Research Institute , Karnal-132001 (Haryana), India .
Amol A
AA
Sahare
2 Animal Biotechnology Centre, National Dairy Research Institute , Karnal-132001 (Haryana), India .
Anuj K
AK
Raja
2 Animal Biotechnology Centre, National Dairy Research Institute , Karnal-132001 (Haryana), India .
Karn P
KP
Singh
2 Animal Biotechnology Centre, National Dairy Research Institute , Karnal-132001 (Haryana), India .
Suresh K
SK
Singla
2 Animal Biotechnology Centre, National Dairy Research Institute , Karnal-132001 (Haryana), India .
Manmohan S
MS
Chauhan
2 Animal Biotechnology Centre, National Dairy Research Institute , Karnal-132001 (Haryana), India .
Radhey S
RS
Manik
2 Animal Biotechnology Centre, National Dairy Research Institute , Karnal-132001 (Haryana), India .
Prabhat
P
Palta
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002020:Buffaloes / Q000196:embryology* / Q000235:genetics*; D019976:Cloning, Organism; D044127:Epigenesis, Genetic; D015870:Gene Expression; D006657:Histones / Q000378:metabolism; D008745:Methylation; D008892:Milk / Q000166:cytology*; D012867:Skin / Q000166:cytology*
D006657:Histones