A new phosphate-starvation response in fission yeast requires the endocytic function of myosin I.
Endocytosis is essential for uptake of many substances into the cell, but how it links to nutritional signalling is poorly understood. Here, we show a new role for endocytosis in regulating the response to low phosphate in Schizosaccharomyces pombe. Loss of function of myosin I (Myo1), Sla2/End4 or Arp2, proteins involved in the early steps of endocytosis, led to increased proliferation in low-phosphate medium compared to controls. We show that once cells are deprived of phosphate they undergo a quiescence response that is dependent on the endocytic function of Myo1. Transcriptomic analysis revealed a wide perturbation of gene expression with induction of stress-regulated genes upon phosphate starvation in wild-type but not Δmyo1 cells. Thus, endocytosis plays a pivotal role in mediating the cellular response to nutrients, bridging the external environment and internal molecular functions of the cell.
Nuclear Dynamics, Babraham Institute, Cambridge CB22 3AT, USA.
Edoardo
E
Petrini
Nuclear Dynamics, Babraham Institute, Cambridge CB22 3AT, USA.
Victoire
V
Baillet
Nuclear Dynamics, Babraham Institute, Cambridge CB22 3AT, USA.
Jake
J
Cridge
Nuclear Dynamics, Babraham Institute, Cambridge CB22 3AT, USA.
Cassandra J
CJ
Hogan
Nuclear Dynamics, Babraham Institute, Cambridge CB22 3AT, USA.
Cindy
C
Guillaume
Nuclear Dynamics, Babraham Institute, Cambridge CB22 3AT, USA.
Huiling
H
Ke
Nuclear Dynamics, Babraham Institute, Cambridge CB22 3AT, USA.
Elisa
E
Brandetti
Imaging Facility, Babraham Institute, Cambridge CB22 3AT, USA.
Simon
S
Walker
Nuclear Dynamics, Babraham Institute, Cambridge CB22 3AT, USA.
Hashem
H
Koohy
Nuclear Dynamics, Babraham Institute, Cambridge CB22 3AT, USA.
Mikhail
M
Spivakov
Nuclear Dynamics, Babraham Institute, Cambridge CB22 3AT, USA patrick.varga-weisz@babraham.ac.uk.
Patrick
P
Varga-Weisz
D051377:Actin-Related Protein 2 / Q000235:genetics / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D059467:Transcriptome
D051377:Actin-Related Protein 2; D010710:Phosphates; D029702:Schizosaccharomyces pombe Proteins; C506560:Sla2 protein, S pombe; C491828:myo1 protein, S pombe; D018995:Myosin Heavy Chains
Endocytosis; Myosin; Phosphate sensing