Gynura procumbens Reverses Acute and Chronic Ethanol-Induced Liver Steatosis through MAPK/SREBP-1c-Dependent and -Independent Pathways.
The present study aimed to evaluate the hepatoprotective effect and mechanism of action of Gynura procumbens on acute and chronic ethanol-induced liver injuries. Ethanol extract from G. procumbens stems (EEGS) attenuated acute ethanol-induced serum alanine aminotransferase levels and hepatic lipid accumulation. Therefore, EEGS was successively extracted by petroleum, ethyl acetate, and n-butyl alcohol. The results showed that the n-butyl alcohol extract was the active fraction of EEGS, and hence it was further fractionated on a polyamide glass column. The 60% ethanol-eluted fraction that contained 13.6% chlorogenic acid was the most active fraction, and its effect was further evaluated using a chronic model. Both the n-butyl alcohol extract and the 60% ethanol-eluted fraction inhibited chronic ethanol-induced hepatic lipid accumulation by modulating lipid metabolism-related regulators through MAPK/SREBP-1c-dependent and -independent signaling pathways and ameliorated liver steatosis. Our findings suggest that EEGS and one of its active ingredients, chlorogenic acid, may be developed as potential effective agents for ethanol-induced liver injury.
Department of Pharmacology, College of Pharmacy, South-Central University for Nationalities , No. 182, Minyuan Road, 430074 Wuhan, China.
Xiao-Jun
XJ
Li
Department of Pharmacology, College of Pharmacy, South-Central University for Nationalities , No. 182, Minyuan Road, 430074 Wuhan, China.
Yun-Mei
YM
Mu
Department of Pharmacology, College of Pharmacy, South-Central University for Nationalities , No. 182, Minyuan Road, 430074 Wuhan, China.
Ting-Ting
TT
Li
Department of Pharmacology, College of Pharmacy, South-Central University for Nationalities , No. 182, Minyuan Road, 430074 Wuhan, China.
Yan-Ling
YL
Yang
Department of Pharmacology, College of Pharmacy, South-Central University for Nationalities , No. 182, Minyuan Road, 430074 Wuhan, China.
Mei-Tuo
MT
Zhang
Department of Pharmacology, College of Pharmacy, South-Central University for Nationalities , No. 182, Minyuan Road, 430074 Wuhan, China.
Yu-Sang
YS
Li
Department of Pharmacology, College of Pharmacy, South-Central University for Nationalities , No. 182, Minyuan Road, 430074 Wuhan, China.
Wei Kevin
WK
Zhang
Department of Pharmacology, College of Pharmacy, South-Central University for Nationalities , No. 182, Minyuan Road, 430074 Wuhan, China.|Key Laboratory of Chinese Internal Medicine of MOE and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine , 100700 Beijing, China.
He-Bin
HB
Tang
Key Laboratory of Chinese Internal Medicine of MOE and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine , 100700 Beijing, China.
Hong-Cai
HC
Shang
D000208:Acute Disease / Q000628:therapy; D000818:Animals; D002908:Chronic Disease / Q000628:therapy; D004195:Disease Models, Animal; D000431:Ethanol / Q000009:adverse effects*; D005234:Fatty Liver / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism*
D051780:Sterol Regulatory Element Binding Protein 1; D000431:Ethanol; D020929:Mitogen-Activated Protein Kinase Kinases
Gynura procumbens; MAPK; SREBP-1c; alcoholic liver disease; chlorogenic acid; steatosis