Copaiba oil effect on induced fecal peritonitis in rats.
OBJECTIVE
To evaluate the effects of copaiba oil as a prophylactic and/or therapeutic substance on survival of rats subjected to cecal ligation and puncture, describing histopathological and oxidative stress findings.


METHODS
Forty rats (Ratus norvegicus) were distributed into five study groups (N=8): Sham group (ShG): normal standard animals; Sepse group (SepG): submitted a cecal ligation and puncture (CLP); Pre group (PreG): administered copaiba oil once daily by subcutaneous injection for five days before carrying out CLP; Post CLP group (PostG): administered copaiba oil once daily by subcutaneous injection from the first day of CLP until death by sepsis; and Pre/Post group (Pre/PostG): administered copaiba oil once daily by subcutaneous injection for five days before carrying out CLP and from the first day of CLP until de death by sepsis. After the death of the animals, blood was collected for assessment of oxidative stress and histological analysis were performed. The Kaplan-Meier curves of surviving time were realized.


RESULTS
Survival analysis demonstrated that animals treated with copaiba oil prior to the execution of the CLP (PreG and Pre/Post groups) had longer survival compared to the sepsis group (p<0.0001) whereas animals receiving copaiba only after the completion of CLP (PostG) showed no statistically significant difference compared to the sepsis group. However, when comparing the two groups in which was administered copaiba previously (PreG and Pre/PostG groups), there was no statistical significance between the groups (p=0.4672). There was no statistical difference between histopathological findings or the levels of oxidative stress.


CONCLUSIONS
Prophylactic subcutaneous administration of copaiba increases survival of rats subjected to severe sepsis by cecal ligation and puncture.
Universidade Estadual do Pará, Belém, PA, BR.
Letícia Nobre
LN
Lopes
School of Medicine, UEPA, BR.
Felipe Augusto Folha
FA
Santos
School of Medicine, UEPA, BR.
Louize Caroline Marques
LC
Oliveira
School of Medicine, Universidade Estadual do Pará, BR.
Sandro
S
Percário
School of Medicine, UEPA, Belém, PA, BR.
Charles Alberto Villacorta de
CA
Barros
School of Medicine, UEPA, Belém, PA, BR.
Marcus Vinicius Henriques
MV
Brito
D000818:Animals; D002432:Cecum / Q000601:surgery; D004195:Disease Models, Animal; D007887:Fabaceae / Q000737:chemistry*; D005243:Feces; D007279:Injections, Subcutaneous; D008026:Ligation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D010538:Peritonitis / Q000188:drug therapy* / Q000209:etiology / Q000517:prevention & control; D010938:Plant Oils / Q000494:pharmacology / Q000627:therapeutic use*; D056990:Post-Exposure Prophylaxis / Q000379:methods; D011677:Punctures; D011897:Random Allocation; D017208:Rats, Wistar; D015203:Reproducibility of Results; D018805:Sepsis / Q000188:drug therapy* / Q000517:prevention & control; D016019:Survival Analysis; D013997:Time Factors; D016896:Treatment Outcome
D010938:Plant Oils; D008315:Malondialdehyde