Outcomes from Cyanoacrylate Use in Tracheal Anastomosis in a Rat Model.
OBJECTIVE
We aimed to investigate the histologic effects of N-butyl-2-cyanoacrylate (NBCA) and metacryloxisulfolane (MS) comonomer on tracheal anastomosis in vivo on a rat model of tracheal resection and end-to-end anastomosis.


METHODS
Animal experiment. Rat model of tracheal resection and end-to-end anastomosis.


METHODS
Animal laboratory.


METHODS
Tracheal resection and end-to-end anastomosis were performed in 28 Wistar albino rats. Rats were randomly divided into 2 groups: control (n = 14), in which anastomoses were performed with 8/0 Vicryl, and study (n = 14), in which NBCA and MS comonomer was used in addition to 8/0 Vicryl to perform anastomosis. Seven rats from each group were sacrificed on day 7 and the remaining on day 28 for gross and histologic examination.


RESULTS
Indurations at the site of anastomoses were detected in all rats of study group. Slight narrowing at the site of anastomosis was observed in 8 rats of the study group and in only 1 of the control group. The difference was significant on day 28 (P = .021). The inflammatory cell infiltration was significantly increased in the study group on day 28 (P = .022). Epithelial regeneration was found to be worse in the study group on day 28 (P = .022).


CONCLUSIONS
The use of NBCA and MS comonomer increased the inflammation in rat tracheal anastomosis with respect to inflammatory cell count and epithelial regeneration. So the use of NBCA and MS comonomer in tracheal surgery cannot be recommended yet in the light of our data.
Department of Otolaryngology, Faculty of Medicine, Abant Izzet Baysal University, Bolu, Turkey ozgur.bicer@ibu.edu.tr.
Yusuf Ozgur
YO
Bicer
Department of Otolaryngology, Faculty of Medicine, Abant Izzet Baysal University, Bolu, Turkey.
Serap
S
Koybasi
Department of Otolaryngology, Faculty of Medicine, Abant Izzet Baysal University, Bolu, Turkey.
Sinan
S
Seyhan
Department of Histology and Embryology, Faculty of Medicine, Abant Izzet Baysal University, Bolu, Turkey.
Tulin
T
Firat
Department of Otolaryngology, Faculty of Medicine, Abant Izzet Baysal University, Bolu, Turkey.
Hasan
H
Kazaz
D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D003487:Cyanoacrylates / Q000494:pharmacology*; D004195:Disease Models, Animal; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013536:Suture Techniques; D014132:Trachea / Q000601:surgery*; D014135:Tracheal Stenosis / Q000601:surgery*; D014945:Wound Healing
D003487:Cyanoacrylates; C079573:3-methoxybutylcyanoacrylate
anastomosis; cyanoacrylate; inflammation; trachea; wound healing