Fast Differential Analysis of Propolis Using Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry.
Mass spectral fingerprints of 24 raw propolis samples, including 23 from China and one from the United States, were directly obtained using surface desorption atmospheric pressure chemical ionization mass spectrometry (SDAPCI-MS) without sample pretreatment. Under the optimized experimental conditions, the most abundant signals were detected in the mass ranges of 70 to 500 m/z and 200 to 350 m/z, respectively. Principal component analyses (PCA) for the two mass ranges showed similarities in that the colors had a significant correlation with the first two PCs; in contrast there was no correlation with the climatic zones from which the samples originated. Analytes such as chrysin, pinocembrin, and quercetin were detected and identified using multiple stage mass spectrometry within 3 min. Therefore, SDAPCI-MS can be used for rapid and reliable high-throughput analysis of propolis.
School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, China.
Xue-Yong
XY
Huang
School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, China.
Xia-Li
XL
Guo
School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, China.
Huo-Lin
HL
Luo
Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang, Jiangxi 330013, China.
Xiao-Wei
XW
Fang
Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang, Jiangxi 330013, China.
Teng-Gao
TG
Zhu
Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang, Jiangxi 330013, China.
Xing-Lei
XL
Zhang
Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang, Jiangxi 330013, China.
Huan-Wen
HW
Chen
School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, China.
Li-Ping
LP
Luo