Intrinsic carbapenem-hydrolyzing oxacillinases from members of the genus Pandoraea.
We analyzed the oxacillinases of isolates of six different species of Pandoraea, a genus that colonizes the respiratory tract of cystic fibrosis patients. The isolates produced carbapenem-hydrolyzing enzymes causing elevated MICs for amoxicillin, piperacillin, meropenem, and imipenem when expressed in an Escherichia coli host strain. Sequencing revealed nine new oxacillinases (OXA-151 to OXA-159) with a high degree of identity among isolates of the same species; however, they had much lower interspecies similarities. The intrinsic oxacillinase genes might therefore be helpful for correct identification of Pandoraea isolates.
MICOER Institute, Munich, Germany schneider_ines@gmx.net.
Ines
I
Schneider
MICOER Institute, Munich, Germany.
Adolf
A
Bauernfeind
D000658:Amoxicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology*; D015378:Imipenem / Q000494:pharmacology; D010878:Piperacillin / Q000494:pharmacology; D001618:beta-Lactamases / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D015780:Carbapenems; D015378:Imipenem; D000658:Amoxicillin; D001618:beta-Lactamases; C108449:oxacillinase; D010878:Piperacillin