Studies on Cytotoxic Activity against HepG-2 Cells of Naphthoquinones from Green Walnut Husks of Juglans mandshurica Maxim.
Twenty-seven naphthoquinones and their derivatives, including four new naphthalenyl glucosides and twenty-three known compounds, were isolated from green walnut husks, which came from Juglans mandshurica Maxim. The structures of four new naphthalenyl glucosides were elucidated based on extensive spectroscopic analyses. All of these compounds were evaluated for their cytotoxic activities against the growth of human cancer cells lines HepG-2 by MTT [3-(4,5-dimethylthiazo l-2-yl)-2,5 diphenyl tetrazolium bromide] assay. The results were shown that most naphthoquinones in an aglycone form exhibited better cytotoxicity in vitro than naphthalenyl glucosides with IC50 values in the range of 7.33-88.23 μM. Meanwhile, preliminary structure-activity relationships for these compounds were discussed.
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. zhouyuanyuan1998@163.com.
Yuanyuan
Y
Zhou
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. ybywater@163.com.
Bingyou
B
Yang
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. jiangyanqiu219@163.com.
Yanqiu
Y
Jiang
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. liu_zhao_xi@163.com.
Zhaoxi
Z
Liu
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. ZYC19901014@126.com.
Yuxin
Y
Liu
College of Adult Education, Heilongjiang University of Chinese Medicine, Harbin 150040, China. wangxiaolicando2000@126.com.
Xiaoli
X
Wang
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. hxkuang@hotmail.com.
Haixue
H
Kuang
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D056945:Hep G2 Cells; D006801:Humans; D031324:Juglans / Q000737:chemistry*; D015394:Molecular Structure; D009285:Naphthoquinones / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship
D000972:Antineoplastic Agents, Phytogenic; D009285:Naphthoquinones; D010936:Plant Extracts
Juglans mandshurica Maxim; cytotoxic activity; green walnut husks; naphthoquinones; structure-activity relationships