Sunlight-driven reduction of silver ion to silver nanoparticle by organic matter mitigates the acute toxicity of silver to Daphnia magna.
Due to the unique antibacterial activities, silver nanoparticles (AgNPs) have been extensively used in commercial products. Anthropogenic activities have released considerable AgNPs as well as highly toxic silver ion (Ag(+)) into the aquatic environment. Our recent study revealed that ubiquitous natural organic matter (NOM) could reduce Ag(+) to AgNP under natural sunlight. However, the toxic effect of this process is not well understood. In this work, we prepared mixture solution of Ag(+) and AgNPs with varied Ag(+)% through the sunlight-driven reduction of Ag(+) by NOM and investigated the acute toxicity of the solutions on Daphnia magna. Formation of AgNPs was demonstrated and characterized by comprehensive techniques and the fraction of unconverted Ag(+) was determined by ultrafiltration-inductively coupled plasma mass spectrometry determination. The formation of AgNPs enhanced significantly with the increasing of solution pH and cumulative photosynthetically active radiation of sunlight. The toxicity of the resulting solution was further investigated by using freshwater crustacean D. magna as a model and an 8hr-median lethal concentration (LC50) demonstrated that the reduction of Ag(+) by NOM to AgNPs significantly mitigated the acute toxicity of silver. These results highlight the importance of sunlight and NOM in the fate, transformation and toxicity of Ag(+) and AgNPs, and further indicate that the acute toxicity of AgNPs should be mainly ascribed to the dissolved Ag(+) from AgNPs.
School of the Environment, Jiangsu University, Zhenjiang 212013, China; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: zhangzhen@ujs.edu.cn.
Zhen
Z
Zhang
School of the Environment, Jiangsu University, Zhenjiang 212013, China; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Xiaoya
X
Yang
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Mohai
M
Shen
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: ygyin@rcees.ac.cn.
Yongguang
Y
Yin
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Jingfu
J
Liu
D000818:Animals; D003621:Daphnia / Q000187:drug effects*; D006812:Humic Substances; D007477:Ions / Q000737:chemistry / Q000528:radiation effects / Q000633:toxicity; D053768:Metal Nanoparticles / Q000737:chemistry / Q000528:radiation effects / Q000633:toxicity*; D010084:Oxidation-Reduction; D012834:Silver / Q000737:chemistry / Q000528:radiation effects / Q000633:toxicity*; D013472:Sunlight; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000528:radiation effects / Q000633:toxicity*
D006812:Humic Substances; D007477:Ions; D014874:Water Pollutants, Chemical; D012834:Silver
Acute toxicity; Daphnia magna; Natural organic matter; Reduction; Silver ion; Silver nanoparticle