Antiallergic Effects of Trichostatin A in a Murine Model of Allergic Rhinitis.
OBJECTIVE
Trichostatin A (TSA), an inhibitor of histone deacetylase, has been shown to play an important role in attenuating asthmatic inflammation. However, the effect of TSA in allergic rhinitis is not known. The aims of this study were to investigate the effect of TSA on allergic nasal inflammation and on the induction of regulatory T cells in a murine model of allergic rhinitis.


METHODS
BALB/c mice were sensitized intraperitoneally with ovalbumin (OVA) and then challenged intranasally with OVA. TSA (1 mg/kg) was given to the treatment group, and multiple parameters of allergic responses were evaluated to determine the effects of TSA on allergic rhinitis. Allergic nasal symptom scores, including frequency of rubbing and sneezing, were checked. Eosinophil infiltrations were stained with Chromotrope 2R, and the expression levels of OVA-specific IgE, T-helper 1 (Th1) cytokine (interferon-gamma [IFN-γ]), Th2 cytokines (interleukin [IL] 4 and IL-5) and Treg (Foxp3, IL-10, and transforming growth factor-beta [TGF-β]) were measured by quantitative reverse transcription-polymerase chain reaction or enzyme-linked immunosorbent assay.


RESULTS
TSA reduced the scores of allergic nasal symptoms and the amount of eosinophil infiltration into the nasal mucosa. TSA suppressed OVA-specific IgE levels and reduced expression of the IL-4 and IL-5. However, the expression of IFN-γ was unchanged in the treatment group. The levels of Foxp3, IL-10, and TGF-β were increased in pretreatment with TSA as compared to control group.


CONCLUSIONS
This study shows that TSA induced antiallergic effects by decreasing eosinophilic infiltration and Th2 cytokines in a murine model of allergic rhinitis via regulation of Tregs. Thus, TSA may be considered a potentially therapeutic agent in treating allergic rhinitis.
Brain Korea 21 Plus for Biomedical Science, Seoul, Korea. ; Institute for Medical Devices Clinical Trial Center, Seoul, Korea.
Jung-Sun
JS
Cho
Brain Korea 21 Plus for Biomedical Science, Seoul, Korea.
Ju-Hyung
JH
Kang
Brain Korea 21 Plus for Biomedical Science, Seoul, Korea.
In-Hye
IH
Han
Brain Korea 21 Plus for Biomedical Science, Seoul, Korea.
Ji-Young
JY
Um
Institute for Medical Devices Clinical Trial Center, Seoul, Korea. ; Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Korea.
Il-Ho
IH
Park
Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Korea.
Sang Hag
SH
Lee
Brain Korea 21 Plus for Biomedical Science, Seoul, Korea. ; Institute for Medical Devices Clinical Trial Center, Seoul, Korea. ; Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Korea.
Heung-Man
HM
Lee
Allergic Rhinitis; Histone Deacetylases; Mice; Ovalbumin; Trichostatin A