Comparative genomic hybridization on microarray (a-CGH) in olfactory neuroblastoma: Analysis of ten cases and review of the literature.
Olfactory neuroblastoma is a rare tumor arising from the basal layer of the olfactory epithelium in the superior recesses of the nasal cavity. The rarity of this tumor, and the difficulties in culturing tumor cells has limited the generation of conventional cytogenetic data, whereas consistent results have been obtained by recent molecular methods. We report the results of an array-based comparative genomic hybridization analysis (a-CGH) obtained on 11 samples from 10 subjects: 8 primary and 3 relapsed tumors. In one patient, both the primary and relapsed tumors were available. Our results on chromosome imbalances highlight the highly heterogeneous presentation: six of eleven samples showed multiple numerical changes and very few structural ones, while four samples showed an opposite pattern; one sample out of eleven showed no imbalances. We did not reach firm evidence of any recurrent specific imbalances either at level of entire chromosomes or chromosome segments. A review of the literature indicates a number of recurrent gains, and losses, mostly not confirmed by our results. Gain of chromosome 19 was the only correspondence with literature data concerning an entire chromosome, and most segmental gains and losses found in our cohort of patients were different from those indicated in the literature: the only similarities concerned the gain of 20q13 and the loss of segments of chromosomes 15 and 22.
Dipartimento Di Medicina Clinica E Sperimentale, Università Degli Studi Dell'insubria, Varese, Italy.
Roberto
R
Valli
Unit of Otorhinolaryngology, Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
Francesca
F
De Bernardi
Dipartimento Di Medicina Clinica E Sperimentale, Università Degli Studi Dell'insubria, Varese, Italy.|Istituto Di Ricerca Genetica E Biomedica, Consiglio Nazionale Delle Ricerche (CNR), Milano, Italy.
Annalisa
A
Frattini
Unit of Otorhinolaryngology, Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
Luca
L
Volpi
Unit of Otorhinolaryngology, Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
Maurizio
M
Bignami
Department of Pathology, University of Brescia, I Servizio Di Anatomia Patologica, and Division of Hematology, Ospedali Civili Di Brescia, Brescia, Italy.
Fabio
F
Facchetti
Dipartimento Di Medicina Clinica E Sperimentale, Università Degli Studi Dell'insubria, Varese, Italy.
Francesco
F
Pasquali
Unit of Otorhinolaryngology, Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
Paolo
P
Castelnuovo
Dipartimento Di Medicina Clinica E Sperimentale, Università Degli Studi Dell'insubria, Varese, Italy.
Emanuela
E
Maserati
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D002884:Chromosomes, Human, Pair 15; D002888:Chromosomes, Human, Pair 19; D002892:Chromosomes, Human, Pair 22; D055028:Comparative Genomic Hybridization / Q000379:methods; D018304:Esthesioneuroblastoma, Olfactory / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009296:Nasal Cavity / Q000473:pathology*; D009364:Neoplasm Recurrence, Local / Q000235:genetics; D009669:Nose Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D035583:Rare Diseases / Q000235:genetics*