Molecular characterization and new geographical record of Lecithochirium priacanthi (Digenea: Hemiuridae) infecting the moontail bullseye fish Priacanthus hamrur (Perciformes: Priacanthidae) from the Red Sea, Egypt.
Sixty specimens of the moontail bullseye Priacanthus hamrur were collected at Coasts of Suez Gulf, Red Sea (Egypt) during the four different seasons of the whole year 2014 and necropsied to study the infection with metazoan parasites. Twenty-one out of 60 examined fish specimens (infection rate of 33.33 %) were found to be naturally infected by the hemiurid digenean parasite Lecithochirium priacanthi. The large-sized fish reaching 15-30 (23.5 ± 4.8) cm were more intensively infected than the smaller ones. A definite seasonal effect was observed as winter was found to be the season of severe parasitic infections, while midsummer was the lowest one. The morphological and morphometric characterization of this parasite were examined by light and scanning electron microscopy. The adult worms had an elongated body measuring 1.93-2.54 (2.11 ± 0.20) mm in length and 0.61-0.72 (0.67 ± 0.02) mm in width. The body was characterized by the presence of a sub-terminal oral sucker with diameters reaching 0.12-0.16 (0.14 ± 0.02) mm. The ventral sucker measured 0.32-0.45 (0.38 ± 0.02) mm in diameter. The body was supplied by a short retracted portion with a blunt end that measured 0.48-0.61 (0.56 ± 0.02) mm in length and 0.28-0.35 (0.32 ± 0.02) mm in width. Morphological results of the present parasite were compared with other related species described previously from Perciformes. Molecular characterization based on small subunit ribosomal DNA was done to confirm the obtained morphological and morphometric results. A preliminary genetic comparison between SSU rDNA of this parasite and other species of Hemiuridae places the present specimen as a putative sister taxon to Lecithochirium grandiporum and Lecithochirium caesionis. The finding of L. priacanthi in Egyptian marine water fish represents a new geographical record for this parasite.
Zoology Department, Faculty of Science, Cairo University, Cairo, Egypt. fathyghaffar@yahoo.com.
Fathy
F
Abdel-Ghaffar
Zoology Department, Faculty of Science, Cairo University, Cairo, Egypt.
Rewaida
R
Abdel-Gaber
Zoology Department, Faculty of Science, Cairo University, Cairo, Egypt.
Abdel-Rahman
AR
Bashtar
Zoology Department, Faculty of Science, Cairo University, Cairo, Egypt.
Kareem
K
Morsy
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Saleh
S
Al Quraishy
Zoology Department, Faculty of Science, Cairo University, Cairo, Egypt.
Rehab
R
Saleh
Parasitology Institute, Düsseldorf University, Düsseldorf, Germany.
Heinz
H
Mehlhorn
D000818:Animals; D002590:Cestode Infections / Q000469:parasitology / Q000662:veterinary*; D004275:DNA, Ribosomal / Q000235:genetics; D004534:Egypt; D005260:Female; D005393:Fish Diseases / Q000469:parasitology*; D019156:Indian Ocean; D008297:Male; D010473:Perciformes / Q000469:parasitology; D010986:Platyhelminths / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D013045:Species Specificity
D004275:DNA, Ribosomal
Hemiuridae; Lecithochirium species; Morphology; Phylogenetic analysis; Priacanthus hamrur fish