Two-dimensional liquid chromatography and its application in traditional Chinese medicine analysis and metabonomic investigation.
Two-dimensional liquid chromatography has become an attractive analytical tool for the separation of complex samples due to its enhanced selectivity, peak capacity, and resolution compared with one-dimensional liquid chromatography. Recently, more attention has been drawn on the application of this separation technique in studies concerning traditional Chinese medicines, metabonomes, proteomes, and other complex mixtures. In this review, we aim to examine the application of two-dimensional liquid chromatography in traditional Chinese medicine analysis and metabonomic investigation. The classification and evaluation indexes were first introduced. Then, various switching methods were summarized when used in an on-line two-dimensional liquid chromatography system. Finally, the applications of this separation technique in traditional Chinese medicine analysis and metabonomic investigation were discussed on the basis of specific studies.
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, China.
Zheng
Z
Li
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, China.
Kai
K
Chen
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, China.|Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical College, Xuzhou, China.
Meng-zhe
MZ
Guo
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, China.|Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical College, Xuzhou, China.
Dao-quan
DQ
Tang
D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D006801:Humans; D008516:Medicine, Chinese Traditional; D055432:Metabolomics / Q000379:methods*
D004365:Drugs, Chinese Herbal
Interfaces; Metabonomics; Orthogonality; Traditional Chinese medicine; Two-dimensional liquid chromatography