A three-dimensional hierarchical scaffold fabricated by a combined rapid prototyping technique and electrospinning process to expand hematopoietic stem/progenitor cells.
OBJECTIVE
To investigate the expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood using extracellular matrix (ECM) protein-coated three-dimensional hierarchical scaffolds.


RESULTS
The expansion of HSPCs was evaluated through total nucleated cell (TNC) expansion, immuno-phenotypic analysis, and clonogenic ability. After 7 days of culture, three-dimensional cultures with fibronectin-coated scaffolds achieved the highest fold increase in TNCs (164 ± 6.9 fold) and the highest CD45(+)CD34(+) (35 %) and CD34(+)CD38(-) (32 %) ratios.


CONCLUSIONS
Three-dimensional hierarchical scaffolds were coated with ECM protein to simulate a biomimetic environment or niche, and had a significant effect on the expansion potential of HSPCs without changing their phenotype.
Department of Biomedical Engineering, Inje University, Rm #309, BLDG-A, 607 Obang-Dong, Gimhae, Gyeongnam, 621-749, Republic of Korea.
Yun Gyeong
YG
Kang
Department of Biomedical Engineering, Inje University, Rm #309, BLDG-A, 607 Obang-Dong, Gimhae, Gyeongnam, 621-749, Republic of Korea.
Ji Won
JW
Shin
Department of Biomedical Engineering, Inje University, Rm #309, BLDG-A, 607 Obang-Dong, Gimhae, Gyeongnam, 621-749, Republic of Korea.
So Hee
SH
Park
Department of Biomedical Engineering, Inje University, Rm #309, BLDG-A, 607 Obang-Dong, Gimhae, Gyeongnam, 621-749, Republic of Korea.
Young Mi
YM
Kim
Department of Health Science and Technology, Inje University, Gimhae, Gyeongnam, Republic of Korea.
Seo Rin
SR
Gu
Department of Health Science and Technology, Inje University, Gimhae, Gyeongnam, Republic of Korea.
Yanru
Y
Wu
Department of Biomedical Engineering, Inje University, Rm #309, BLDG-A, 607 Obang-Dong, Gimhae, Gyeongnam, 621-749, Republic of Korea.
Hun Yeong
HY
Ban
Department of Biomedical Engineering, Inje University, Rm #309, BLDG-A, 607 Obang-Dong, Gimhae, Gyeongnam, 621-749, Republic of Korea. biomechshin@gmail.com.|Department of Health Science and Technology, Inje University, Gimhae, Gyeongnam, Republic of Korea. biomechshin@gmail.com.|Cardiovascular and Metabolic Disease Center/Institute of Aged Life Redesign/UHARC, Inje University, Gimhae, Republic of Korea. biomechshin@gmail.com.
Jung-Woog
JW
Shin
D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D049109:Cell Proliferation; D005353:Fibronectins / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D017493:Leukocyte Common Antigens / Q000378:metabolism; D055153:Stem Cell Niche; D013499:Surface Properties; D014470:Umbilical Cord / Q000166:cytology*
D018952:Antigens, CD34; D001672:Biocompatible Materials; D005353:Fibronectins; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human; D051997:ADP-ribosyl Cyclase 1
Electrospinning; Ex vivo expansion; Hematopoietic stem/progenitor cell; Hierarchical scaffold; Rapid prototyping; Stem cell niche