Transplantation of umbilical cord blood mononuclear cells increases levels of nerve growth factor in the cerebrospinal fluid of patients with autism.
We aimed to evaluate the levels of growth factors in the cerebrospinal fluid (CSF) of patients with autism after transplantation of umbilical cord blood mononuclear cells (CBMNCs). Fourteen subjects diagnosed with autism received transplantation of CBMNCs first through intravenous infusion, and three times subsequently through intrathecal injections. A 2-mL sample of CSF was taken before each intrathecal injection. CSF levels of nerve growth factor (NGF), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF) were determined by enzyme-linked immunosorbent assay. All data are reported as means ± SD and were analyzed using the SPSS 10.0 software. One-way analysis of variance with post-hoc F-and Q-tests were performed for comparisons. NGF levels in the CSF were significantly increased after transplantation (213.54 ± 56.38 after the third versus 28.32 ± 12.22 ng/L after the first transplantation; P < 0.05), while VEGF and bFGF levels did not change significantly. Therefore, transplantation of CBMNCs could increase NGF levels in the CSF of patients with autism.
Shandong University, Jinan, China.
Q
Q
Li
Shandong Provincial Hospital, Jinan, China.
C-F
CF
Chen
Shandong Jiaotong Hospital, Jinan, China.
D-Y
DY
Wang
Shandong Jiaotong Hospital, Jinan, China.
Y-T
YT
Lü
Shandong Jiaotong Hospital, Jinan, China.
Y
Y
Huan
Shandong Jiaotong Hospital, Jinan, China.
M
M
Liu
Shandong Jiaotong Hospital, Jinan, China.
R-C
RC
Ge
Shandong Jiaotong Hospital, Jinan, China.
X-W
XW
Chen
Shandong Jiaotong Hospital, Jinan, China.
H-S
HS
Qi
Shandong Jiaotong Hospital, Jinan, China.
L
L
An
Shenzhen Beike Biotechnology Co. Ltd, Shenzhen, China.
X
X
Hu
D001321:Autistic Disorder / Q000134:cerebrospinal fluid* / Q000628:therapy; D002648:Child; D002675:Child, Preschool; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D016222:Fibroblast Growth Factor 2 / Q000134:cerebrospinal fluid; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000637:transplantation*; D008297:Male; D020932:Nerve Growth Factor / Q000134:cerebrospinal fluid*; D016896:Treatment Outcome; D042461:Vascular Endothelial Growth Factor A / Q000134:cerebrospinal fluid
C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D020932:Nerve Growth Factor