Conserved Cis-Regulatory Modules Control Robustness in Msx1 Expression at Single-Cell Resolution.
The process of transcription is highly stochastic leading to cell-to-cell variations and noise in gene expression levels. However, key essential genes have to be precisely expressed at the correct amount and time to ensure proper cellular development and function. Studies in yeast and bacterial systems have shown that gene expression noise decreases as mean expression levels increase, a relationship that is controlled by promoter DNA sequence. However, the function of distal cis-regulatory modules (CRMs), an evolutionary novelty of metazoans, in controlling transcriptional robustness and variability is poorly understood. In this study, we used live cell imaging of transfected reporters combined with a mathematical modelling and statistical inference scheme to quantify the function of conserved Msx1 CRMs and promoters in modulating single-cell real-time transcription rates in C2C12 mouse myoblasts. The results show that the mean expression-noise relationship is solely promoter controlled for this key pluripotency regulator. In addition, we demonstrate that CRMs modulate single-cell basal promoter rate distributions in a graded manner across a population of cells. This extends the rheostatic model of CRM action to provide a more detailed understanding of CRM function at single-cell resolution. We also identify a novel CRM transcriptional filter function that acts to reduce intracellular variability in transcription rates and show that this can be phylogenetically separable from rate modulating CRM activities. These results are important for understanding how the expression of key vertebrate developmental transcription factors is precisely controlled both within and between individual cells.
Department of Biology and Biochemistry, University of Bath, United Kingdom Warwick Systems Biology Centre, University of Warwick, Coventry, United Kingdom School of Life Sciences, University of Warwick, Coventry, United Kingdom k.w.vance@bath.ac.uk g.koentges@warwick.ac.uk.
Keith W
KW
Vance
Warwick Systems Biology Centre, University of Warwick, Coventry, United Kingdom.
Dan J
DJ
Woodcock
MRC Biostatistics Unit, Robinson Way, Cambridge, United Kingdom.
John E
JE
Reid
Warwick Systems Biology Centre, University of Warwick, Coventry, United Kingdom.
Till
T
Bretschneider
Warwick Systems Biology Centre, University of Warwick, Coventry, United Kingdom.
Sascha
S
Ott
School of Life Sciences, University of Warwick, Coventry, United Kingdom k.w.vance@bath.ac.uk g.koentges@warwick.ac.uk.
Georgy
G
Koentges
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017124:Conserved Sequence; D005786:Gene Expression Regulation; D051957:MSX1 Transcription Factor / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008957:Models, Genetic; D011401:Promoter Regions, Genetic; D050436:Regulatory Elements, Transcriptional; D059010:Single-Cell Analysis; D014158:Transcription, Genetic
D051957:MSX1 Transcription Factor; C484084:Msx1 protein, mouse
Msx1; cis-regulatory module; promoter; robustness; single-cell transcription