Non-parental banding patterns in recombinant inbred line population of maize with SSR markers.
We observed 3 types of non-parental banding patterns using simple-sequence repeat primers in a recombinant inbred line maize population developed from 2 inbred lines, Mo17 and KW7. We observed alleles that were not present in either of the parents, known as non-parental alleles. Although non-parental alleles are a consequence of genetic variation, they are less common in progenies derived from inbred lines. Generally, when non-parental alleles are encountered during genotyping analysis, they are either deleted from the analysis or considered to be missing data. However, before making a decision regarding how to treat non-parental alleles, it is important to understand the mechanism through which they form. There are a variety of potential reasons for the formation of non-parental bands, including recombination or mutation in the simple-sequence repeat region, residual heterozygosity in parental lines, or chromosomal aberrations resulting from rearrangements and transposons. In this article, we discuss the potential reasons behind the formation of the non-parental alleles observed in our data.
Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Korea.
R V
RV
Ramekar
Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Korea.
K J
KJ
Sa
Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Korea.
S Y
SY
Woo
Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Korea.
J K
JK
Lee
D000483:Alleles; D002871:Chromosome Banding; D004251:DNA Transposable Elements / Q000235:genetics; D015321:Gene Rearrangement; D014644:Genetic Variation / Q000235:genetics; D007178:Inbreeding; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D003313:Zea mays / Q000235:genetics*
D004251:DNA Transposable Elements