Inactivation of Burkholderia pseudomallei on environmental surfaces using spray-applied, common liquid disinfectants.
Five commercially available liquid antimicrobials were evaluated for their ability to decontaminate common environmental surface materials, contaminated with Burkholderia pseudomallei, using a spray-based disinfectant delivery procedure. Tests were conducted at both an ambient temperature (c. 20°C) and a lower temperature (c. 12°C) condition. Nonporous materials (glass and aluminium) were more easily decontaminated than porous materials (wood, concrete and carpet). Citric acid (1%) demonstrated poor efficacy in all test conditions. Bleach (pH-adjusted), ethanol (70%), quaternary ammonium and PineSol®, demonstrated high (>6 log10 reduction) efficacies on glass and aluminium at both temperatures, but achieved varying results for wood, carpet and concrete. Temperature had minimal effect on decontamination efficacy during these tests.


CONCLUSIONS
Much of the antimicrobial efficacy data for pathogenic micro-organisms are generated with testing that utilizes hard nonporous surface materials. These data are not directly translatable for decontaminant selection following an incident whereby complex and porous environmental surfaces are contaminated. This study presents efficacy data for spray-applied antimicrobial liquids, when used to decontaminate common environmental surfaces contaminated with Burkholderia pseudomallei. These data can help responders develop effective remediation strategies following an environmental contamination incident involving B. pseudomallei.
Office of Research and Development, National Homeland Security Research Center, U.S. EPA, Research Triangle Park, NC, USA.
M W
http://orcid.org/0000-0001-6544-329X
MW
Calfee
Battelle Memorial Institute, Columbus, OH, USA.
M
M
Wendling
D016957:Burkholderia pseudomallei / Q000187:drug effects*; D019343:Citric Acid / Q000494:pharmacology; D003666:Decontamination / Q000379:methods*; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods*; D000431:Ethanol / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013696:Temperature
D004202:Disinfectants; D000644:Quaternary Ammonium Compounds; D019343:Citric Acid; D000431:Ethanol; D012973:Sodium Hypochlorite
Burkholderia pseudomallei; biological agent; decontamination; disinfection; inactivation