Zoonotic Bartonella species in wild rodents in the state of Mato Grosso do Sul, Brazil.
Several rodent-associated Bartonella species cause disease in humans but little is known about their epidemiology in Brazil. The presence of Bartonella spp. in wild rodents captured in two municipalities of the Mato Grosso do Sul state was assessed by polymerase chain reaction (PCR). Fragments of heart tissue from 42 wild rodents were tested using primers targeting the Bartonella 16S-23S intergenic transcribed spacer (ITS) region and citrate synthase gltA gene. The wild rodents were identified based on external and cranial morphology and confirmed at species level by mitochondrial DNA (cytochrome B) sequencing and karyotype. Overall, 42.9% (18/42) of the wild rodents were PCR positive for Bartonella spp.: Callomys callosus (04), Cerradomys maracajuensis (04), Hylaeamus megacephalus (01), Necromys lasiurus (06), Nectomys squamipes (01), Oecomys catherinae (01) and Oxymycterus delator (01). Bartonella vinsonii subsp. arupensis was detected in N. lasiurus (46%) and C. callosus (21%) captured in the two study sites. We reported the first molecular detection of B. vinsonii subsp. arupensis in different species of wild rodents collected in the Brazilian territory. Further studies are needed to examine the role of these mammals in the eco-epidemiology of bartonellosis in Brazil.
Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz, (FIOCRUZ), Rio de Janeiro-RJ, Brazil. Electronic address: afavacho@ioc.fiocruz.br.
Alexsandra Rodrigues de Mendonça
AR
Favacho
Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz, (FIOCRUZ), Rio de Janeiro-RJ, Brazil.
Marcelle Novaes
MN
Andrade
Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz, (FIOCRUZ), Rio de Janeiro-RJ, Brazil.
Renata Carvalho
RC
de Oliveira
Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz, (FIOCRUZ), Rio de Janeiro-RJ, Brazil; Instituto Nacional do Câncer (INCA), Rio de Janeiro-RJ, Brazil.
Cibele Rodrigues
CR
Bonvicino
Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz, (FIOCRUZ), Rio de Janeiro-RJ, Brazil.
Paulo Sergio
PS
D'Andrea
Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz, (FIOCRUZ), Rio de Janeiro-RJ, Brazil.
Elba Regina Sampaio
ER
de Lemos
D000818:Animals; D001473:Bartonella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001474:Bartonella Infections / Q000453:epidemiology* / Q000382:microbiology / Q000662:veterinary*; D001938:Brazil / Q000453:epidemiology; D002950:Citrate (si)-Synthase / Q000235:genetics; D045303:Cytochromes b / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D006321:Heart / Q000382:microbiology; D058889:Molecular Typing; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012377:Rodentia / Q000382:microbiology*
D021901:DNA, Intergenic; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D045303:Cytochromes b; D002950:Citrate (si)-Synthase
Bartonella vinsonii subsp. arupensis; Brazil; Heart; Wild rodents