Synthesis and optimization of wide pore superficially porous particles by a one-step coating process for separation of proteins and monoclonal antibodies.
Superficially porous particles (SPPs) with pore size ranging from 90Å to 120Å have been a great success for the fast separation of small molecules over totally porous particles in recent years. However, for the separation of large biomolecules such as proteins, particles with large pore size (e.g. ≥ 300Å) are needed to allow unrestricted diffusion inside the pores. One early example is the commercial wide pore (300Å) SPPs in 5μm size introduced in 2001. More recently, wide pore SPPs (200Å and 400Å) in smaller particle sizes (3.5-3.6μm) have been developed to meet the need of increasing interest in doing faster analysis of larger therapeutic molecules by biopharmaceutical companies. Those SSPs in the market are mostly synthesized by the laborious layer-by-layer (LBL) method. A one step coating approach would be highly advantageous, offering potential benefits on process time, easier quality control, materials cost, and process simplicity for facile scale-up. A unique one-step coating process for the synthesis of SPPs called the "coacervation method" was developed by Chen and Wei as an improved and optimized process, and has been successfully applied to synthesis of a commercial product, Poroshell 120 particles, for small molecule separation. In this report, we would like to report on the most recent development of the one step coating coacervation method for the synthesis of a series of wide pore SPPs of different particle size, pore size, and shell thickness. The one step coating coacervation method was proven to be a universal method to synthesize SPPs of any particle size and pore size. The effects of pore size (300Å vs. 450Å), shell thickness (0.25μm vs. 0.50μm), and particle size (2.7μm and 3.5μm) on the separation of large proteins, intact and fragmented monoclonal antibodies (mAbs) were studied. Van Deemter studies using proteins were also conducted to compare the mass transfer properties of these particles. It was found that the larger pore size actually had more impact on the performance of mAbs than particle size and shell thickness. The SPPs with larger 3.5μm particle size and larger 450Å pore size showed the best resolution of mAbs and the lowest back pressure. To the best of our knowledge, this is the largest pore size made on SPPs. These results led to the optimal particle design with a particle size of 3.5μm, a thin shell of 0.25μm and a larger pore size of 450Å.
Agilent Technologies, Inc., 2850 Centerville Road, Wilmington, Delaware 19808, USA. Electronic address: wu_chen@agilent.com.
Wu
W
Chen
Agilent Technologies, Inc., 2850 Centerville Road, Wilmington, Delaware 19808, USA.
Kunqiang
K
Jiang
Agilent Technologies, Inc., 2850 Centerville Road, Wilmington, Delaware 19808, USA.
Anne
A
Mack
Agilent Technologies, Inc., 2850 Centerville Road, Wilmington, Delaware 19808, USA.
Bo
B
Sachok
Agilent Technologies, Inc., 2850 Centerville Road, Wilmington, Delaware 19808, USA.
Xin
X
Zhu
Agilent Technologies, Inc., 2850 Centerville Road, Wilmington, Delaware 19808, USA.
William E
WE
Barber
Agilent Technologies, Inc., 2850 Centerville Road, Wilmington, Delaware 19808, USA.
Xiaoli
X
Wang
D000911:Antibodies, Monoclonal / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis; D010316:Particle Size; D010455:Peptides / Q000032:analysis; D016062:Porosity; D011506:Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D010455:Peptides; D011506:Proteins
Core–shell particles; Fused-core particles; Monoclonal antibody separation; One step coating; Protein separations; Superficially porous particles; Wide pores