A Locked Nucleic Acid Probe Based on Selective Salt-Induced Effect Detects Single Nucleotide Polymorphisms.
Detection of single based genetic mutation by using oligonucleotide probes is one of the common methods of detecting single nucleotide polymorphisms at known loci. In this paper, we demonstrated a hybridization system which included a buffer solution that produced selective salt-induced effect and a locked nucleic acid modified 12 nt oligonucleotide probe. The hybridization system is suitable for hybridization under room temperature. By using magnetic nanoparticles as carriers for PCR products, the SNPs (MDR1 C3435T/A) from 45 volunteers were analyzed, and the results were consistent with the results from pyrophosphoric acid sequencing. The method presented in this paper differs from the traditional method of using molecular beacons to detect SNPs in that it is suitable for research institutions lacking real-time quantitative PCR detecting systems, to detect PCR products at room temperature.
Department of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China.
Jing
J
Zhang
Department of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China.
Huizhe
H
Wu
Department of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China.
Qiuchen
Q
Chen
Department of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China.
Pengfei
P
Zhao
Department of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China.
Haishan
H
Zhao
Department of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China.
Weifan
W
Yao
Department of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China.
Minjie
M
Wei
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide
C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D015342:DNA Probes