Multiple-response optimization of the acidic treatment of the brown alga Ecklonia radiata for the sequential extraction of fucoidan and alginate.
The aim of this study was to optimize the acidic treatment of the brown alga Ecklonia radiata in order to extract fucoidan and facilitate the efficient sequential extraction of alginates. Response surface methodology was used to determine the effects of the temperature, pH, and duration of the acidic treatment on fucoidan yield, alginate extractability, and the molecular weight of sequentially extracted alginates. Desirability functions were then used to predict the best overall combinations of responses. The most desirable compromise allowed for the recovery of a fucoidan-rich fraction with a yield of 3.75% (w/w of alga) and the sequential extraction of alginates having an average molecular weight of 730kDa at a yield of 44% (w/w of alga), with low cross-contamination between the products. The optimized acidic treatment could form the basis of an industrial biorefinery process for the production of both fucoidan and alginate.
Centre for Marine Bioproducts Development, School of Medicine, Flinders University, Bedford Park, South Australia 5042, Australia; Department of Medical Biotechnology, School of Medicine, Flinders University, Bedford Park, South Australia 5042, Australia.
Andrew John
AJ
Lorbeer
ARC Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, University of Adelaide, Adelaide, South Australia 5064, Australia.
Jelle
J
Lahnstein
ARC Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, University of Adelaide, Adelaide, South Australia 5064, Australia.
Vincent
V
Bulone
Centre for Marine Bioproducts Development, School of Medicine, Flinders University, Bedford Park, South Australia 5042, Australia; Department of Medical Biotechnology, School of Medicine, Flinders University, Bedford Park, South Australia 5042, Australia.
Trung
T
Nguyen
Centre for Marine Bioproducts Development, School of Medicine, Flinders University, Bedford Park, South Australia 5042, Australia; Department of Medical Biotechnology, School of Medicine, Flinders University, Bedford Park, South Australia 5042, Australia. Electronic address: wei.zhang@flinders.edu.au.
Wei
W
Zhang
D000464:Alginates / Q000737:chemistry / Q000302:isolation & purification*; D005591:Chemical Fractionation / Q000379:methods*; D020723:Glucuronic Acid / Q000737:chemistry / Q000302:isolation & purification; D006603:Hexuronic Acids / Q000737:chemistry / Q000302:isolation & purification; D008970:Molecular Weight; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification*
D000464:Alginates; D006603:Hexuronic Acids; D011134:Polysaccharides; D020723:Glucuronic Acid; C007789:fucoidan
Biorefinery; Desirability function; Experimental design; Response surface methodology; Sulfated polysaccharides