Expression profile of eight glutathione S-transferase genes in Crassostrea ariakensis after exposure to DSP toxins producing dinoflagellate Prorocentrum lima.
In this study, changes in eight GSTs mRNA level including GST-α, GST-σ, GST-ω, GST-π, GST-μ, GST-ρ, GST-θ and microsomal GST (mGST) in the oyster Crassostrea ariakensis after exposure to Prorocentrum lima have been evaluated by quantitative real-time PCR. Additionally, the contents of five GST isoforms were detected by ELISA. After exposure to P. lima at density of 2 × 10(5) cells/L, mGST mRNA significantly increased in gill, while GST-σ was induced in digestive gland. After exposure to P. lima at density of 2 × 10(6) cells/L, GST-ω and mGST expressions increased in gill, whereas GST-α and GST-σ were induced in digestive gland. The GST content and activity in oysters exposed to P. lima also showed a different pattern when the different isoforms and organs were compared. After exposure to P. lima (2 × 10(6) cell/L), GST-π increased in gill but decreased in digestive gland. The total GST enzyme activity increased in gill, while remained unchanged in digestive gland. These various regulation of GST gene expressions indicated that the GSTs isoenzymes might play divergent physiological roles in the detoxification of DSP toxins in C. ariakensis.
Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Ying
Y
Zou
Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Xiao-Meng
XM
Wei
Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Hui-Wen
HW
Weng
Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Hong-Ye
HY
Li
Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Jie-Sheng
JS
Liu
Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address: tywd@jnu.edu.cn.
Wei-Dong
WD
Yang
D000818:Animals; D049896:Crassostrea / Q000187:drug effects* / Q000235:genetics; D004141:Dinoflagellida / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000235:genetics*; D008387:Marine Toxins / Q000633:toxicity*; D012333:RNA, Messenger / Q000235:genetics
D008387:Marine Toxins; D012333:RNA, Messenger; D005982:Glutathione Transferase
Crassostrea ariakensis; Diarrhetic shellfish poisoning toxins; Glutathione S-transferase; Okadaic acid