Immuno-Electron Microscopy and Electron Microscopic In Situ Hybridization for Visualizing piRNA Biogenesis Bodies in Drosophila Ovaries.
Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specific proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specific target labeled with both fluorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.
Department of Physiology, School of Medicine, Keio University, Shinanomachi 35, Shinjuku-ku, Tokyo, 160-8582, Japan, shibata@2001.jukuin.keio.ac.jp.
Shinsuke
S
Shibata
Yukiko
Y
Murota
Yoshinori
Y
Nishimoto
Mana
M
Yoshimura
Toshihiro
T
Nagai
Hideyuki
H
Okano
Mikiko C
MC
Siomi
D000818:Animals; D004331:Drosophila melanogaster; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D016253:Microscopy, Immunoelectron / Q000379:methods*; D010053:Ovary / Q000648:ultrastructure*; D034741:RNA, Small Interfering / Q000235:genetics
D034741:RNA, Small Interfering