Interisland variegation of venom [Lys(49)]phospholipase A2 isozyme genes in Protobothrops genus snakes in the southwestern islands of Japan.
Protobothrops tokarensis (Pt), a Crotalinae snake, inhabits only Takarajima and Kodakarajima islands of the Tokara Islands located in the immediate north of Amami-Oshima island of Japan. Kodakarajima P. tokarensis venom gland cDNA library gave four types of phospholipase A2 (PLA2) cDNAs encoding neutral [Asp(49)]PLA2, basic [Asp(49)]PLA2, highly basic [Asp(49)]PLA2, and [Lys(49)]PLA2. As the amino acid sequences encoded by their open reading frames (ORFs) were identical to those of PLA2, PLA-B, PLA-N, and BPI (a [Lys(49)]PLA2), respectively, from Amami-Oshima P. flavoviridis (Pf) venom, they were named PtPLA2, PtPLA-B, PtPLA-N, and PtBPI. Chromatography of P. tokarensis venom gave three PLA2 isozymes, PtPLA2, PtPLA-B, and PtBPI. However, BPII and BPIII ([Lys(49)]PLA2s) expressed in Amami-Oshima P. flavoviridis venom were not found in P. tokarensis venom. Genomic polymerase chain reaction (PCR) for P. tokarensis liver DNAs with the unique primers gave PtBPI gene. Notably it was found that LINE (long interspersed nuclear element)-1 fragment is inserted into second intron of PtBPI gene. The LINE-1 fragment may prevent duplication of PtBPI gene and thus formation of plural [Lys(49)]PLA2 genes in P. tokarensis genome. The interisland variegation of venom [Lys(49)]PLA2 isozyme genes in Protobothrops genus snakes in the southwestern islands of Japan is discussed.
Department of Applied Life Science, Faculty of Bioscience, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
Kazuaki
K
Yamaguchi
Department of Applied Life Science, Faculty of Bioscience, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan. Electronic address: chijiwa@life.sojo-u.ac.jp.
Takahito
T
Chijiwa
Department of Applied Life Science, Faculty of Bioscience, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
Takeshi
T
Yamamura
Department of Applied Life Science, Faculty of Bioscience, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
Naoki
N
Ikeda
Department of Applied Life Science, Faculty of Bioscience, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
Takayo
T
Yatsui
Department of Applied Life Science, Faculty of Bioscience, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
Setsuko
S
Hayama
Institute of Medical Science, University of Tokyo, Oshima-gun, Kagoshima 894-1531, Japan.
Shosaku
S
Hattori
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
Naoko
N
Oda-Ueda
Department of Applied Life Science, Faculty of Bioscience, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
Motonori
M
Ohno
D000220:Adaptation, Biological; D000595:Amino Acid Sequence; D000818:Animals; D003435:Crotalid Venoms / Q000201:enzymology*; D015723:Gene Library; D005838:Genotype; D062312:Islands; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics; D007564:Japan; D008969:Molecular Sequence Data; D054467:Phospholipases A2 / Q000737:chemistry / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D012934:Social Isolation; D017819:Viperidae / Q000235:genetics*
D003435:Crotalid Venoms; D007527:Isoenzymes; D054467:Phospholipases A2
APOBEC; Genome; LINE-1; Phospholipase A(2) isozymes; Protobothrops tokarensis; Venom