Novel approach to fast determination of cholesterol oxidation products in Cypriot foodstuffs using ultra-performance liquid chromatography-tandem mass spectrometry.
This paper reports the development and validation of a new method based on ultra-performance LC coupled to MS/MS for the simultaneous determination of four cholesterol oxidation products (COPs) in foodstuffs in only 4.1 min. The COPs were detected by ESI in positive-ion mode with multiple reaction monitoring, and the mass spectrometric conditions were optimized in order to increase sensitivity. The developed method was validated in terms of linearity, precision, LODs, and LOQs. Recoveries of the extraction process ranged from 86 to 98.5% when the samples were fortified at 100, 500, and 1500 ng/mL. The applicability of the method was confirmed by analyzing different food samples. Considering the paucity of data regarding the content of COPs in Cypriot foods, particular attention was devoted, for the first time, to the determination of the profile of the main COPs in widely consumed, traditional Cypriot foodstuffs (halloumi cheese, hiromeri, snails, etc.).
Department of Chemistry, University of Cyprus, Nicosia, Cyprus.
Christiana A
CA
Georgiou
Department of Chemistry, University of Cyprus, Nicosia, Cyprus.
Michalis S
MS
Constantinou
Department of Chemistry, University of Cyprus, Nicosia, Cyprus.
Rafaella
R
Andreou
Department of Civil and Environmental Engineering, University of Cyprus, Nicosia, Cyprus.
Evroula
E
Hapeshi
Department of Civil and Environmental Engineering, University of Cyprus, Nicosia, Cyprus.
Despo
D
Fatta-Kassinos
Department of Chemistry, University of Cyprus, Nicosia, Cyprus.
Constantina P
CP
Kapnissi-Christodoulou
D000818:Animals; D002784:Cholesterol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003535:Cyprus; D003611:Dairy Products / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D008460:Meat / Q000032:analysis; D010084:Oxidation-Reduction; D011817:Rabbits; D015203:Reproducibility of Results; D012908:Snails; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
D002784:Cholesterol
Cholesterol oxidation products; Cypriot food products; Tandem mass spectrometry; Ultra-performance liquid chromatography