Genetic analysis and chromosome mapping of resistance toFusarium oxysporumf. sp.niveum(FON) race 1 and race 2 in watermelon (Citrullus lanatusL.).
Fusarium wilt (FW) caused byFusarium oxysporumf. sp. niveum(FON) is the major soilborne disease of watermelon (Citrullus lanatusL.). The development and deployment of resistant cultivars is generally considered to be an effective approach to control FW. In this study, an F8 population consisting of 103 recombinant inbred lines derived from a cross between the cultivar 97103 and a wild accession PI 296341-FR was used for FON race 1 and race 2 fungal inoculations. One major QTL on chromosome 1 for FON race 1 resistance was detected with a logarithm of odds of 13.2 and explained phenotypic variationR2= 48.1 %; two QTLs of FON race 2 resistance on chromosomes 9 and 10 were discovered based on the high-density integrated genetic map we constructed. The nearest molecular marker should be useful for marker-assisted selection of FON race 1 and race 2 resistance. One receptor kinase, one glucan endo-1,3-β-glucosidase precursors and three acidic chitinase located in the FON-1 QTL genomic region. InQfon2.1QTL region, one lipoxygenase gene, five receptor-like kinases and four glutathione S-transferase genes are discovered. One arginine biosynthesis bifunctional protein, two receptor kinase proteins and one lipid-transfer protein located inQfon2.2QTL region. Based on SNP analysis by using 20 re-sequenced accessions of watermelon and 231-plant F2population generated from Black Diamond × Calhoun Grey, we developed a SNP marker Chr1SNP_502124 for FON-1 detection.
National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, People's Republic of China ; Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing, People's Republic of China ; Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, People's Republic of China ; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Yi
Y
Ren
National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, People's Republic of China ; Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing, People's Republic of China ; Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, People's Republic of China ; Beijing University of Agriculture, Beijing, People's Republic of China.
Di Jiao
National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, People's Republic of China ; Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing, People's Republic of China ; Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, People's Republic of China.
Guoyi
G
Gong
National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, People's Republic of China ; Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing, People's Republic of China ; Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, People's Republic of China.
Haiying
H
Zhang
National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, People's Republic of China ; Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing, People's Republic of China ; Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, People's Republic of China.
Shaogui
S
Guo
National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, People's Republic of China ; Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing, People's Republic of China ; Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, People's Republic of China.
Jie
J
Zhang
National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, People's Republic of China ; Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing, People's Republic of China ; Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, People's Republic of China.
Yong
Y
Xu
Fusarium wilt; QTL; Watermelon