Genetic Variants of PTPN2 Gene in Chinese Children with Type 1 Diabetes Mellitus.
BACKGROUND
Several studies have reported the association of PTPN2 gene with type 1 diabetes mellitus (T1DM) in many populations but not in the Chinese Han population. Therefore, the goal of our study was to replicate the reported association between 2 single-nucleotide polymorphisms (SNPs; rs478582 and rs2542151) in the PTPN2 gene and T1DM in Chinese Han children.


METHODS
This case-control study included 141 Chinese Han children with T1DM and 282 healthy controls. Genetic variants of rs478582 and rs2542151 in PTPN2 gene were performed by PCR amplification followed by restriction fragment length polymorphism method.


RESULTS
No difference was observed in association of rs478582 in The PTPN2 gene and T1DM. The distribution of allele frequency of rs2542151 differed significantly between T1DM patients and healthy controls (OR, 0.6; 95%CI: 0.44 to 0.95; and P=0.024). Dominant model of rs254215 also was associated with T1DM (OR, 0.6; 95%CI: 0.40 to 0.96; and P=0.032). Younger age at onset in G carriers appeared to increase the risk for T1DM (P=0.030).


CONCLUSIONS
The findings suggested that rs2542151 SNP in The PTPN2 gene was associated with T1DM in Chinese Han children. Further studies with larger sample sizes involving gene-gene interactions are urgently needed.
Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China (mainland).
Hui
H
Peng
Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China (mainland).
Jiamei
J
Li
Department of Pediatrics, Taian Central Hospital, Taian, Shandong, China (mainland).
Xiaoyun
X
Chen
Department of pediatrics, Shandong Provincial Hospital affiliated to Shandong University, Jinan, China (mainland).
Xiao
X
Zhou
Department of Pediatrics, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong, China (mainland).
Weiwei
W
Zhu
Department of Pediatrics, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong, China (mainland).
Feng
F
Li
D000293:Adolescent; D000367:Age Factors; D000483:Alleles; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002681:China; D003922:Diabetes Mellitus, Type 1 / Q000208:ethnology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006579:Heterozygote; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D054578:Protein Tyrosine Phosphatase, Non-Receptor Type 2 / Q000235:genetics*
C516649:PTPN2 protein, human; D054578:Protein Tyrosine Phosphatase, Non-Receptor Type 2