Prooxidant activity of norbixin in model of acute gastric ulcer induced by ethanol in rats.
Free radicals and oxidative stress play a central role in gastric injuries caused by ethanol (EtOH). Antioxidant strategies to counteract EtOH toxicity are highly desirable. Norbixin (NBIX) is a carotenoid with antioxidant potential largely used in the food industry. This study evaluated the NBIX effects in a model of gastric ulcer induced by EtOH in rats. Male Wistar rats received NBIX doses of 0, 10, and 25 mg/kg by gavage 1 h after EtOH administration (0 or 75% solution, 1 mL/200 g of animal). The animals were euthanized 1 h after the NBIX administration, and their stomachs were removed for macroscopic and histopathological analyses, quantification of nonprotein sulfhydryl (NPSH) groups, lipid peroxidation (LPO) levels, and catalase (CAT) activity determination. NBIX increased LPO in gastric mucosa and caused CAT inhibition and NPSH depletion in EtOH-treated animals. Results showed that NBIX did not protect gastric tissue against EtOH damage, and this could be associated to a prooxidant effect.
Post-Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
B T
BT
Rovani
Post-Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
R B
RB
de Freitas
Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil paularaugusti@gmail.com.
P R
PR
Augusti
Post-Graduate Program in Pharmacology, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
I C
IC
Araldi
Post-Graduate Program in Pharmacology, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
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S
Somacal
Integrated Center for Laboratory Analysis Development (NIDAL), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
A
A
Quatrin
Integrated Center for Laboratory Analysis Development (NIDAL), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
T
T
Emanuelli
Department of Pathology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
M P
MP
da Rocha
Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
L de Freitas
Lde F
Bauermann
D000208:Acute Disease; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002338:Carotenoids / Q000008:administration & dosage / Q000627:therapeutic use / Q000633:toxicity*; D002374:Catalase / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity*; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced* / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D013438:Sulfhydryl Compounds / Q000378:metabolism
D000975:Antioxidants; D013438:Sulfhydryl Compounds; D002338:Carotenoids; D000431:Ethanol; C099823:norbixin; D002374:Catalase
Oxidative stress; antioxidants; carotenoids; lipid peroxidation