Enhancing Quantum Dots for Bioimaging using Advanced Surface Chemistry and Advanced Optical Microscopy: Application to Silicon Quantum Dots (SiQDs).
Fluorescence lifetime imaging microscopy is successfully demonstrated in both one- and two-photon cases with surface modified, nanocrystalline silicon quantum dots in the context of bioimaging. The technique is further demonstrated in combination with Förster resonance energy transfer studies where the color of the nanoparticles is tuned by using organic dye acceptors directly conjugated onto the nanoparticle surface.
School of Chemistry, Australian Centre for NanoMedicine, ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of New South Wales, Sydney, NSW, 2052, Australia.
Xiaoyu
X
Cheng
EMBL Australia Node in Single Molecule Science, School of Medical Sciences, ARC Centre of Excellence in Advanced Molecular Imaging, The University of New South Wales, Sydney, NSW, 2052, Australia.
Elizabeth
E
Hinde
EMBL Australia Node in Single Molecule Science, School of Medical Sciences, ARC Centre of Excellence in Advanced Molecular Imaging, The University of New South Wales, Sydney, NSW, 2052, Australia.
Dylan M
DM
Owen
School of Chemistry, Australian Centre for NanoMedicine, ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of New South Wales, Sydney, NSW, 2052, Australia.
Stuart B
SB
Lowe
School of Physics, The University of New South Wales, Sydney, NSW, 2052, Australia.
Peter J
PJ
Reece
EMBL Australia Node in Single Molecule Science, School of Medical Sciences, ARC Centre of Excellence in Advanced Molecular Imaging, The University of New South Wales, Sydney, NSW, 2052, Australia.
Katharina
K
Gaus
School of Chemistry, Australian Centre for NanoMedicine, ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of New South Wales, Sydney, NSW, 2052, Australia.
J Justin
JJ
Gooding
D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D045663:Quantum Dots / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
D012825:Silicon
Förster resonance energy transfer (FRET); cancer; fluorescence; fluorescence lifetime imaging microscopy (FLIM); quantum dots