Physical localization of molecular markers and assignment of the 15th linkage group to chromosome 11 of the karyotype in cassava (Manihot esculenta Crantz) by primed in situ labeling.
Physical localization of molecular markers and assignment of the 15th linkage group to chromosome 11 of the karyotype in cassava (Manihot esculenta Crantz) were achieved using primed in situ labeling. Amplified signals for both the EST507-1 and SSRY13-5 markers were consistently observed in different stages of cell division. A comparison of the length, arm ratio, and other morphological characteristics of somatic metaphase chromosomes in karyotype analysis indicated that the EST507-1 and SSRY13-5 markers were localized on the short and long arm of cassava chromosome 11 with the relative map positions of 41.67 and 23.07, respectively. The physical localization of the 2 markers on chromosome 11 of the karyotype corresponds to their positions on the 15th linkage group in cassava.
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agronomy, Hainan University, Haikou, Hainan Province, China.
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Wang
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agronomy, Hainan University, Haikou, Hainan Province, China.
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Wang
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agronomy, Hainan University, Haikou, Hainan Province, China.
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Yin
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agronomy, Hainan University, Haikou, Hainan Province, China.
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Gao
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agronomy, Hainan University, Haikou, Hainan Province, China.
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Zhuang
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agronomy, Hainan University, Haikou, Hainan Province, China liu3305602@163.com.
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JP
Liu
D002455:Cell Division / Q000235:genetics; D002874:Chromosome Mapping; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D007621:Karyotyping; D002365:Manihot / Q000166:cytology / Q000235:genetics*
D005819:Genetic Markers