Effect of Collagen Matrix Saturation on the Surface Free Energy of Dentin using Different Agents.
The surface free energy of conditioned-dentin is one of the factors that interfere with monomeric infiltration of the interfibrillar spaces. Saturation of the tooth matrix with different substances may modulate this energy and, consequently, the wettability of the dentin.



To evaluate the influence of different substances used to saturate conditioned-dentin on surface free energy (SFE) of this substrate.



Dentin blocks (4 × 7 × 1 mm, n = 6/ group), obtained from the roots of bovine incisors, were etched using phosphoric acid for 15 seconds, rinsed and gently dried. The surfaces were treated for 60 seconds with: ultra-purified water (H20-control); ethanol (EtOH), acetone (ACT), chlorhexidine (CHX), ethylenediaminetetraacetic acid (EDTA); or sodium hypochlorite (NaOCl). The tooth surfaces were once again dried with absorbent paper and prepared for SFE evaluation using three standards: water, formamide and bromonaphthalene. Analysis of variance (ANOVA) and Dunnet's tests (a = 0.05) were applied to the data.



Ethylenediaminetetraacetic acid was the only substance that caused a change to the contact angle for the standards water and formamide, while only EtOH influenced the angles formed between formamide and the dentin surface. None of the substances exerted a significant effect for bromonaphtha-lene. In comparison to the control, only EDTA and NaOCl altered both polar components of the SFE. Total SFE was increased by saturation of the collagen matrix by EDTA and reduced when NaOCl was used.



Saturation of the collagen matrix by EDTA and EtOH changed the surface free energy of the dentin. In addition, the use of NaOCl negatively interfered with the properties evaluated.



The increase of surface free energy and wettability of the dentin surface would allow higher penetration of the the adhesive system, which would be of importance to the clinical success of resin-dentin union.
Department of Restorative Dentistry, Araraquara Dental School, University Estadual Paulista-UNESP, Araraquara Sao Paulo, Brazil.
Leopoldina de Fátima Dantas
Lde F
de Almeida
Department of Prosthodontics and Periodontology Piracicaba Dental School, University of Campinas, Piracicaba Sao Paulo, Brazil.
Samilly Evangelista
SE
Souza
Department of Prosthodontics and Periodontology Piracicaba Dental School, University of Campinas, Piracicaba Sao Paulo, Brazil.
Aline Araújo
AA
Sampaio
Department of Dentistry, State University of Paraiba (UEPB) Campina Grande, Paraiba, Brazil.
Yuri Wanderley
YW
Cavalcanti
Department of Prosthodontics and Periodontology Piracicaba Dental School, University of Campinas, Piracicaba Sao Paulo, Brazil.
Wander José
WJ
da Silva
Department of Prosthodontics and Periodontology Piracicaba Dental School, University of Campinas, Piracicaba Sao Paulo, Brazil.
Altair A
AA
Del Bel Cur
Professor, Department of Orthodontics and Pediatric Dentistry, Ara-raquara Dental School, University Estadual Paulista-UNESP Araraquara Humaita, 1680, Sao Paulo, Brazil, Phone: (+55) 16-3301-6334 e-mail: jhebling@foar.unesp.br.
Josimeri
J
Hebling
D000096:Acetone / Q000494:pharmacology; D000134:Acid Etching, Dental / Q000379:methods; D000818:Animals; D002417:Cattle; D002710:Chlorhexidine / Q000494:pharmacology; D003094:Collagen / Q000187:drug effects* / Q000648:ultrastructure; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D004492:Edetic Acid / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D005559:Formamides / Q000737:chemistry; D008422:Materials Testing; D009281:Naphthalenes / Q000737:chemistry; D010756:Phosphoric Acids / Q000737:chemistry; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology*; D013500:Surface Tension; D014867:Water / Q000737:chemistry; D017155:Wettability
D005559:Formamides; D009281:Naphthalenes; D010756:Phosphoric Acids; D012388:Root Canal Irrigants; D012997:Solvents; D014867:Water; D000096:Acetone; C031721:naphthalene; D000431:Ethanol; C031066:formamide; D003094:Collagen; D004492:Edetic Acid; D012973:Sodium Hypochlorite; C030242:phosphoric acid; D002710:Chlorhexidine
Dentin; Surface properties; Tooth acid-etching; Surface free energy