A reagent for specific recognition of cysteine in aqueous buffer and in natural milk: imaging studies, enzymatic reaction and analysis of whey protein.
We report a new chemodosimetric probe () for specific recognition of cysteine (Cys) in aqueous buffer and in whey protein isolated from fresh cow's milk. Using this reagent we could develop a luminescence-based methodology for estimation of Cys released from a commercially available Cys-supplement drug by aminoacylase-1 in live cells.
Organic Chemistry Division, CSIR-National Chemical Laboratory, Pune - 411008, India. a.das@ncl.res.in.
Anila H
AH
A
Upendar Reddy
UR
G
Firoj
F
Ali
Nandaraj
N
Taye
Samit
S
Chattopadhyay
Amitava
A
Das
D000581:Amidohydrolases / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D003545:Cysteine / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D015394:Molecular Structure; D014867:Water / Q000737:chemistry; D000067816:Whey Proteins / Q000032:analysis*
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D000067816:Whey Proteins; D014867:Water; D000581:Amidohydrolases; C019672:aminoacylase I; D003545:Cysteine