Prenatal Exposure to Sodium Arsenite Alters Placental Glucose 1, 3, and 4 Transporters in Balb/c Mice.
Inorganic arsenic (iAs) exposure induces a decrease in glucose type 4 transporter (GLUT4) expression on the adipocyte membrane, which may be related to premature births and low birth weight infants in women exposed to iAs at reproductive age. The aim of this study was to analyze the effect of sodium arsenite (NaAsO2) exposure on GLUT1, GLUT3, and GLUT4 protein expression and on placental morphology. Female Balb/c mice (n = 15) were exposed to 0, 12, and 20 ppm of NaAsO2 in drinking water from 8th to 18th day of gestation. Morphological changes and GLUT1, GLUT3, and GLUT4 expression were evaluated in placentas by immunohistochemical and image analysis and correlated with iAs and arsenical species concentration, which were quantified by atomic absorption spectroscopy. NaAsO2 exposure induced a significant decrease in fetal and placental weight (P < 0.01) and increases in infarctions and vascular congestion. Whereas GLUT1 expression was unchanged in placentas from exposed group, GLUT3 expression was found increased. In contrast, GLUT4 expression was significantly lower (P < 0.05) in placentas from females exposed to 12 ppm. The decrease in placental GLUT4 expression might affect the provision of adequate fetal nutrition and explain the low fetal weight observed in the exposed groups.
Programa de Maestría en Ciencias en Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito No. 1 Nuevo Campus Universitario, 31125 Chihuahua, CHIH, Mexico.
Daniela Sarahí
DS
Gutiérrez-Torres
Programa de Maestría en Ciencias en Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito No. 1 Nuevo Campus Universitario, 31125 Chihuahua, CHIH, Mexico.
Carmen
C
González-Horta
Departamento de Toxicología, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Avenida Instituto Politécnico Nacional 2508, 07360 Mexico, DF, Mexico.
Luz María
LM
Del Razo
Programa de Maestría en Ciencias en Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito No. 1 Nuevo Campus Universitario, 31125 Chihuahua, CHIH, Mexico.
Rocío
R
Infante-Ramírez
Departamento de Anatomía Patológica del Hospital CIMA, Avenida Hacienda del Valle No. 7120, 31217 Chihuahua, CHIH, Mexico.
Ernesto
E
Ramos-Martínez
Laboratorio de Embriología, Facultad de Medicina, Universidad Autónoma de Chihuahua, Nuevo Campus Universitario, 31125 Chihuahua, CHIH, Mexico.
Margarita
M
Levario-Carrillo
Programa de Maestría en Ciencias en Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito No. 1 Nuevo Campus Universitario, 31125 Chihuahua, CHIH, Mexico.
Blanca
B
Sánchez-Ramírez
D017667:Adipocytes / Q000187:drug effects / Q000473:pathology; D000818:Animals; D018053:Arsenites / Q000633:toxicity*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D051272:Glucose Transporter Type 1 / Q000096:biosynthesis* / Q000235:genetics; D051273:Glucose Transporter Type 2 / Q000096:biosynthesis* / Q000235:genetics; D051275:Glucose Transporter Type 4 / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007230:Infant, Low Birth Weight / Q000378:metabolism; D051379:Mice; D010920:Placenta / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D047928:Premature Birth / Q000139:chemically induced / Q000235:genetics / Q000473:pathology; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced / Q000235:genetics / Q000473:pathology; D017670:Sodium Compounds / Q000633:toxicity*; D013054:Spectrophotometry, Atomic
D018053:Arsenites; D051272:Glucose Transporter Type 1; D051273:Glucose Transporter Type 2; D051275:Glucose Transporter Type 4; C495682:Slc2a2 protein, mouse; D017670:Sodium Compounds; C017947:sodium arsenite; D005947:Glucose