Gut Microbiome Composition in Young Nicaraguan Children During Diarrhea Episodes and Recovery.
Understanding how the gut microbiota is affected by diarrhea episodes may help explain alterations in intestinal function among children in low-income settings. This study examined the composition of the gut microbiome of Nicaraguan children both during diarrhea episodes and while free of diarrhea for at least 2 months. Relative abundances of bacterial taxa, phylogenetic diversity, and species richness were determined by 16S amplicon sequencing and compared between paired diarrhea and recovery samples. A total of 66 stools were provided by 25 children enrolled in a 1-year cohort study of diarrhea etiologies. Children in our cohort had a mean age of 21.9 months; 64% were breast-fed, and 10% had received an antibiotic during the diarrhea episode. Overall, phylogenetic diversity and species richness did not differ significantly between diarrhea and recovery stools. However, of children who had a bacterial enteropathogen detected in any diarrhea stool, none experienced an increase in phylogenetic diversity in recovery, whereas of those in whom no bacterial enteropathogens were detected in their diarrhea stool(s), 59% experienced an increase in phylogenetic diversity in recovery (P = 0.008). This preliminary study suggests that recovery of the gut microbiota after a diarrhea episode may take longer time than previously thought and may be pathogen specific.
Department of Family Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Microbiome Core Facility, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Laboratory of Biochemistry and Immunology, Faculty of Sciences, Mohammed V University, Rabat, Morocco; Department of Microbiology and Parasitology, National Autonomous University of Nicaragua, León, Nicaragua; Department of Biostatistics, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Epidemiology, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Center for Demographic and Health Research, National Autonomous University of Nicaragua, León, Nicaragua; Department of Cell Biology and Physiology, University of North Carolina School of Medicine, Chapel Hill, North Carolina sbd@unc.edu.
Sylvia
S
Becker-Dreps
Department of Family Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Microbiome Core Facility, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Laboratory of Biochemistry and Immunology, Faculty of Sciences, Mohammed V University, Rabat, Morocco; Department of Microbiology and Parasitology, National Autonomous University of Nicaragua, León, Nicaragua; Department of Biostatistics, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Epidemiology, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Center for Demographic and Health Research, National Autonomous University of Nicaragua, León, Nicaragua; Department of Cell Biology and Physiology, University of North Carolina School of Medicine, Chapel Hill, North Carolina.
Imane
I
Allali
Department of Family Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Microbiome Core Facility, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Laboratory of Biochemistry and Immunology, Faculty of Sciences, Mohammed V University, Rabat, Morocco; Department of Microbiology and Parasitology, National Autonomous University of Nicaragua, León, Nicaragua; Department of Biostatistics, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Epidemiology, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Center for Demographic and Health Research, National Autonomous University of Nicaragua, León, Nicaragua; Department of Cell Biology and Physiology, University of North Carolina School of Medicine, Chapel Hill, North Carolina.
Andrea
A
Monteagudo
Department of Family Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Microbiome Core Facility, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Laboratory of Biochemistry and Immunology, Faculty of Sciences, Mohammed V University, Rabat, Morocco; Department of Microbiology and Parasitology, National Autonomous University of Nicaragua, León, Nicaragua; Department of Biostatistics, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Epidemiology, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Center for Demographic and Health Research, National Autonomous University of Nicaragua, León, Nicaragua; Department of Cell Biology and Physiology, University of North Carolina School of Medicine, Chapel Hill, North Carolina.
Samuel
S
Vilchez
Department of Family Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Microbiome Core Facility, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Laboratory of Biochemistry and Immunology, Faculty of Sciences, Mohammed V University, Rabat, Morocco; Department of Microbiology and Parasitology, National Autonomous University of Nicaragua, León, Nicaragua; Department of Biostatistics, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Epidemiology, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Center for Demographic and Health Research, National Autonomous University of Nicaragua, León, Nicaragua; Department of Cell Biology and Physiology, University of North Carolina School of Medicine, Chapel Hill, North Carolina.
Michael G
MG
Hudgens
Department of Family Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Microbiome Core Facility, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Laboratory of Biochemistry and Immunology, Faculty of Sciences, Mohammed V University, Rabat, Morocco; Department of Microbiology and Parasitology, National Autonomous University of Nicaragua, León, Nicaragua; Department of Biostatistics, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Epidemiology, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Center for Demographic and Health Research, National Autonomous University of Nicaragua, León, Nicaragua; Department of Cell Biology and Physiology, University of North Carolina School of Medicine, Chapel Hill, North Carolina.
Elizabeth T
ET
Rogawski
Department of Family Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Microbiome Core Facility, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Laboratory of Biochemistry and Immunology, Faculty of Sciences, Mohammed V University, Rabat, Morocco; Department of Microbiology and Parasitology, National Autonomous University of Nicaragua, León, Nicaragua; Department of Biostatistics, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Epidemiology, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Center for Demographic and Health Research, National Autonomous University of Nicaragua, León, Nicaragua; Department of Cell Biology and Physiology, University of North Carolina School of Medicine, Chapel Hill, North Carolina.
Ian M
IM
Carroll
Department of Family Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Microbiome Core Facility, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Laboratory of Biochemistry and Immunology, Faculty of Sciences, Mohammed V University, Rabat, Morocco; Department of Microbiology and Parasitology, National Autonomous University of Nicaragua, León, Nicaragua; Department of Biostatistics, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Epidemiology, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Center for Demographic and Health Research, National Autonomous University of Nicaragua, León, Nicaragua; Department of Cell Biology and Physiology, University of North Carolina School of Medicine, Chapel Hill, North Carolina.
Luis Enrique
LE
Zambrana
Department of Family Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Microbiome Core Facility, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Laboratory of Biochemistry and Immunology, Faculty of Sciences, Mohammed V University, Rabat, Morocco; Department of Microbiology and Parasitology, National Autonomous University of Nicaragua, León, Nicaragua; Department of Biostatistics, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Epidemiology, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Center for Demographic and Health Research, National Autonomous University of Nicaragua, León, Nicaragua; Department of Cell Biology and Physiology, University of North Carolina School of Medicine, Chapel Hill, North Carolina.
Felix
F
Espinoza
Department of Family Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Microbiome Core Facility, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Laboratory of Biochemistry and Immunology, Faculty of Sciences, Mohammed V University, Rabat, Morocco; Department of Microbiology and Parasitology, National Autonomous University of Nicaragua, León, Nicaragua; Department of Biostatistics, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Epidemiology, University of North Carolina Gillings School of Global Public Health, Chapel Hill, North Carolina; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Center for Demographic and Health Research, National Autonomous University of Nicaragua, León, Nicaragua; Department of Cell Biology and Physiology, University of North Carolina School of Medicine, Chapel Hill, North Carolina.
M Andrea
MA
Azcarate-Peril
D004275:DNA, Ribosomal / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology; D003968:Diarrhea, Infantile / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005260:Female; D000069196:Gastrointestinal Microbiome / Q000235:genetics; D006801:Humans; D007223:Infant; D008297:Male; D009527:Nicaragua / Q000453:epidemiology; D010802:Phylogeny
D004275:DNA, Ribosomal