Analysis of alterations to the transcriptome of Loquat (Eriobotrya japonica Lindl.) under low temperature stress via de novo sequencing.
Loquat (Eriobotrya japonica Lindl.), which originates from the cooler hill regions of southwestern China, is a typical subtropical evergreen tree. Loquat is one of the most important economic crops in China, but the available genomic information is very limited. Here, we present the first deep transcriptomic analysis of loquat. De novo assembly generated 116,723 contigs and 64,814 unigenes using Illumina sequencing technology. A total of 45,739 unigenes were annotated by Nr, GO, and COG datasets. In addition, we analyzed the gene expression profiles of loquat fruit under low temperature stress and 4017 differential expressed genes were identified. We found that the unigenes involved in the brassinosteroid biosynthesis and phosphatidylinositol signaling systems were upregulated, indicating that they have an important role in the resistance of plants to low temperature. Our results provide an invaluable resource for identification of specific genes and proteins involved in loquat development and response to low temperatures.
College of Horticulture, Sichuan Agricultural University, Ya' an, China gongronggao2014@163.com.
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RG
Gong
College of Horticulture, Sichuan Agricultural University, Ya' an, China.
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J
Lai
College of Horticulture, Sichuan Agricultural University, Ya' an, China.
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W
Yang
College of Horticulture, Sichuan Agricultural University, Ya' an, China.
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MA
Liao
College of Horticulture, Sichuan Agricultural University, Ya' an, China.
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ZH
Wang
College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
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GL
Liang
D003080:Cold Temperature; D019295:Computational Biology; D031983:Eriobotrya / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D053858:Metabolic Networks and Pathways; D058977:Molecular Sequence Annotation; D010840:Phytosterols / Q000378:metabolism; D015398:Signal Transduction; D013312:Stress, Physiological; D059467:Transcriptome
D010840:Phytosterols