Diversity and Distribution of Archaea in the Mangrove Sediment of Sundarbans.
Mangroves are among the most diverse and productive coastal ecosystems in the tropical and subtropical regions. Environmental conditions particular to this biome make mangroves hotspots for microbial diversity, and the resident microbial communities play essential roles in maintenance of the ecosystem. Recently, there has been increasing interest to understand the composition and contribution of microorganisms in mangroves. In the present study, we have analyzed the diversity and distribution of archaea in the tropical mangrove sediments of Sundarbans using 16S rRNA gene amplicon sequencing. The extraction of DNA from sediment samples and the direct application of 16S rRNA gene amplicon sequencing resulted in approximately 142 Mb of data from three distinct mangrove areas (Godkhali, Bonnie camp, and Dhulibhashani). The taxonomic analysis revealed the dominance of phyla Euryarchaeota and Thaumarchaeota (Marine Group I) within our dataset. The distribution of different archaeal taxa and respective statistical analysis (SIMPER, NMDS) revealed a clear community shift along the sampling stations. The sampling stations (Godkhali and Bonnie camp) with history of higher hydrocarbon/oil pollution showed different archaeal community pattern (dominated by haloarchaea) compared to station (Dhulibhashani) with nearly pristine environment (dominated by methanogens). It is indicated that sediment archaeal community patterns were influenced by environmental conditions.
Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal 700019, India.
Anish
A
Bhattacharyya
Roche Diagnostics India Pvt. Ltd., Block 4C, Akash Tower, Near Ruby Hospital, 781 Anandapur, Kolkata 700107, India.
Niladri Shekhar
NS
Majumder
Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal 700019, India.
Pijush
P
Basak
Department of Biochemistry, Bose Institute, P1/12, C. I. T. Road, Scheme VIIM, Kolkata, West Bengal 700054, India.
Shayantan
S
Mukherji
Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal 700019, India.
Debojyoti
D
Roy
Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal 700019, India.
Sudip
S
Nag
Department of Geography, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal 700019, India.
Anwesha
A
Haldar
Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal 700019, India.
Dhrubajyoti
D
Chattopadhyay
Norwich Medical School, University of East Anglia and Institute of Food Research, Norwich Research Park, Norwich, Norfolk NR4 7UA, UK.
Suparna
S
Mitra
Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal 700019, India.
Maitree
M
Bhattacharyya
Department of Biochemistry, Bose Institute, P1/12, C. I. T. Road, Scheme VIIM, Kolkata, West Bengal 700054, India.
Abhrajyoti
A
Ghosh
D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D016000:Cluster Analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014329:Tropical Climate; D053833:Wetlands
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S