Site-selective multi-porphyrin attachment enables the formation of a next-generation antibody-based photodynamic therapeutic.
Herein we present a significant step towards next-generation antibody-based photodynamic therapeutics. Site-selective modification of a clinically relevant monoclonal antibody, with a serum-stable linker bearing a strained alkyne, allows for the controlled Cu-free "click" assembly of an in vitro active antibody-based PDT agent using a water soluble azide porpyhrin.
Department of Chemistry, University College London, 20 Gordon Street, London, WC1H 0AJ, UK. v.chudasama@ucl.ac.uk.
Antoine
A
Maruani
Huguette
H
Savoie
Francesca
F
Bryden
Stephen
S
Caddick
Ross
R
Boyle
Vijay
V
Chudasama
D000480:Alkynes / Q000138:chemical synthesis / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015394:Molecular Structure; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011166:Porphyrins / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D000480:Alkynes; D000911:Antibodies, Monoclonal; D017319:Photosensitizing Agents; D011166:Porphyrins