Amplification refractory mutation system polymerase chain reaction versus optimized polymerase chain reaction restriction-fragment length polymorphism for apolipoprotein E genotyping of majorly depressed patients.
Major depressive disorder (MDD) is a prevalent, debilitating mood disorder that has been associated with several genetic polymorphisms. One such polymorphism, namely that of apolipoprotein E (APOE), has three allelic forms (ε2, ε3 and ε4) that encode for six unique isoforms of the APOE protein. A growing number of techniques have been developed for APOE genotyping; however, not all polymerase chain reaction (PCR)‑based genotyping techniques are equally accurate or cost‑effective. In order to find a more accurate and cost‑effective APOE genotyping method for MDD screening in large populations, the present study comparatively evaluated two genotyping methods, amplification refractory mutation system PCR (ARMS‑PCR) and optimized PCR restriction‑fragment length polymorphism (PCR‑RFLP), in blood samples taken from a population of 708 MDD patients. Although either of the two methods were able to detect all six unique APOE genotypes, comparisons of the two methods with Sanger sequencing demonstrated that ARMS‑PCR (94%) was significantly more accurate than optimized PCR‑RFLP (82%). ARMS‑PCR should prove useful in quickly verifying ambiguous results obtained by other APOE genotyping methods and can be cost-effectively performed in the setting of a small laboratory or a population-based screening program.
Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
Hongmin
H
You
Chongqing Key Laboratory of Neurobiology, Chongqing 400016, P.R. China.
Jin
J
Chen
Chongqing Key Laboratory of Neurobiology, Chongqing 400016, P.R. China.
Jingjing
J
Zhou
Chongqing Key Laboratory of Neurobiology, Chongqing 400016, P.R. China.
Hua
H
Huang
Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
Junxi
J
Pan
Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
Ziye
Z
Wang
Chongqing Key Laboratory of Neurobiology, Chongqing 400016, P.R. China.
Lin
L
Lv
Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
Lujun
L
Zhang
Chongqing Key Laboratory of Neurobiology, Chongqing 400016, P.R. China.
Juan
J
Li
Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
Bin
B
Qin
Chongqing Key Laboratory of Neurobiology, Chongqing 400016, P.R. China.
Yongtao
Y
Yang
Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China.
Peng
P
Xie
D000293:Adolescent; D000328:Adult; D000368:Aged; D001057:Apolipoproteins E / Q000235:genetics*; D001483:Base Sequence; D003865:Major Depressive Disorder / Q000175:diagnosis* / Q000235:genetics; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D055815:Young Adult
D001057:Apolipoproteins E