A molecular fluorescent dye for specific staining and imaging of RNA in live cells: a novel ligand integration from classical thiazole orange and styryl compounds.
A new RNA-selective fluorescent dye integrated with a thiazole orange and a p-(methylthio)styryl moiety shows better nucleolus RNA staining and imaging performance in live cells than the commercial stains. It also exhibits excellent photostability, cell tolerance, and counterstain compatibility with 4',6-diamidino-2-phenylindole for specific RNA-DNA colocalization in bioassays.
Institute of Natural Medicine and Green Chemistry, School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, P. R. China. luyj@gdut.edu.cn kzhang@gdut.edu.cn.
Yu-Jing
YJ
Lu
Qiang
Q
Deng
Dong-Ping
DP
Hu
Zheng-Ya
ZY
Wang
Bao-Hua
BH
Huang
Zhi-Yun
ZY
Du
Yan-Xiong
YX
Fang
Wing-Leung
WL
Wong
Kun
K
Zhang
Cheuk-Fai
CF
Chow
D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry*; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D051379:Mice; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D011804:Quinolines / Q000737:chemistry*; D012334:RNA, Neoplasm / Q000032:analysis*; D013343:Styrenes / Q000737:chemistry*
D052160:Benzothiazoles; D005456:Fluorescent Dyes; D008024:Ligands; D011804:Quinolines; D012334:RNA, Neoplasm; D013343:Styrenes; C051000:thiazole orange