Antimicrosporidian activity of sulphated polysaccharides from algae and their potential to control honeybee nosemosis.
Nosemosis is one of the most common and widespread diseases of adult honeybees. The causative agents, Nosema apis and Nosema ceranae, belong to microsporidia some obligate intracellular eukaryotic parasites. In this study, 10 sulphated polysaccharides from algae were evaluated for their antimicrosporidian activity. They were first shown to inhibit the in vitro growth of the mammal microsporidian model, Encephalitozoon cuniculi. The most efficient polysaccharides were then tested for their ability to inhibit the growth of Nosema ceranae in experimentally-infected adult honeybees. Two polysaccharides extracted from Porphyridium spp. did not show any toxicity in honeybees and one of them allowed a decrease of both parasite load and mortality rate due to N. ceranae infection. A decrease in parasite abundance but not in mortality rate was also observed with an iota carrageenan. Our results are promising and suggest that algal sulphated polysaccharides could be used to prevent and/or control bee nosemosis.
Clermont Université, Université Blaise Pascal, Laboratoire "Microorganismes: Génome et Environnement", BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, F-63171 Aubière, France. Electronic address: michael.roussel@univ-bpclermont.fr.
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Roussel
Clermont Université, Université Blaise Pascal, Institut Pascal, TSA 60206, CS 60026, 63178 Aubière, France; CNRS, UMR 6602, IP, F-63171 Aubière, France. Electronic address: aurorevillay@hotmail.fr.
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Villay
Clermont Université, Université Blaise Pascal, Laboratoire "Microorganismes: Génome et Environnement", BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, F-63171 Aubière, France. Electronic address: Frederic.DELBAC@univ-bpclermont.fr.
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Delbac
Clermont Université, Université Blaise Pascal, Institut Pascal, TSA 60206, CS 60026, 63178 Aubière, France; CNRS, UMR 6602, IP, F-63171 Aubière, France. Electronic address: Philippe.MICHAUD@univ-bpclermont.fr.
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Michaud
Clermont Université, Université Blaise Pascal, Institut Pascal, TSA 60206, CS 60026, 63178 Aubière, France; CNRS, UMR 6602, IP, F-63171 Aubière, France. Electronic address: claroche@cust.univ-bpclermont.fr.
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Laroche
Clermont Université, Université Blaise Pascal, Laboratoire "Microorganismes: Génome et Environnement", BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, F-63171 Aubière, France. Electronic address: Diane.RORIZ@univ-bpclermont.fr.
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Roriz
Clermont Université, Université Blaise Pascal, Laboratoire "Microorganismes: Génome et Environnement", BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, F-63171 Aubière, France. Electronic address: hicham.el_alaoui@univ-bpclermont.fr.
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El Alaoui
Clermont Université, Université Blaise Pascal, Laboratoire "Microorganismes: Génome et Environnement", BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, F-63171 Aubière, France. Electronic address: marie.diogon@univ-bpclermont.fr.
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Diogon
D000818:Animals; D000977:Antiparasitic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D001516:Bees / Q000187:drug effects / Q000469:parasitology*; D006801:Humans; D058086:Microalgae / Q000737:chemistry*; D016881:Microsporidiosis / Q000662:veterinary*; D016818:Nosema / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D011817:Rabbits; D012624:Seaweed / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry*
D000977:Antiparasitic Agents; D011134:Polysaccharides; D013431:Sulfates
Algae; Antimicrosporidian activity; Apis mellifera; Honeybee; Nosema ceranae; Polysaccharides