Bioconversion of Pinoresinol Diglucoside and Pinoresinol from Substrates in the Phenylpropanoid Pathway by Resting Cells of Phomopsis sp.XP-8.
Pinoresinol diglucoside (PDG) and pinoresinol (Pin) are normally produced by plant cells via the phenylpropanoid pathway. This study reveals the existence of a related pathway in Phomopsis sp. XP-8, a PDG-producing fungal strain isolated from the bark of the Tu-chung tree (Eucommiaulmoides Oliv.). After addition of 0.15 g/L glucose to Phomopsis sp. XP-8, PDG and Pin formed when phenylalanine, tyrosine, leucine, cinnamic acid, and p-coumaric acid were used as the substrates respectively. No PDG formed in the absence of glucose, but Pin was detected after addition of all these substrates except leucine. In all systems in the presence of glucose, production of PDG and/or Pin and the accumulation of phenylalanine, cinnamic acid, or p-coumaric acid correlated directly with added substrate in a time- and substrate concentration- dependent manner. After analysis of products produced after addition of each substrate, the mass flow sequence for PDG and Pin biosynthesis was defined as: glucose to phenylalanine, phenylalanine to cinnamic acid, then to p-coumaric acid, and finally to Pin or PDG. During the bioconversion, the activities of four key enzymes in the phenylpropanoid pathway were also determined and correlated with accumulation of their corresponding products. PDG production by Phomopsis sp. exhibits greater efficiency and cost effectiveness than the currently-used plant-based system and will pave the way for large scale production of PDG and/or Pin for medical applications.
College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, Yangling, Shaanxi Province, 712100, China.
Yan
Y
Zhang
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, Shaanxi Province, 710072, China.
Junling
J
Shi
College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, Yangling, Shaanxi Province, 712100, China.
Laping
L
Liu
College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, Yangling, Shaanxi Province, 712100, China.
Zhenhong
Z
Gao
College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, Yangling, Shaanxi Province, 712100, China.
Jinxin
J
Che
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, Shaanxi Province, 710072, China.
Dongyan
D
Shao
College of enology, Northwest A&F University, Xinong Road, Yangling, Shaanxi Province, 712100, China.
Yanlin
Y
Liu
D000596:Amino Acids / Q000378:metabolism; D001203:Ascomycota / Q000378:metabolism*; D005663:Furans / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D017705:Lignans / Q000378:metabolism*; D011422:Propionates / Q000378:metabolism*
D000596:Amino Acids; D005663:Furans; D017705:Lignans; D011422:Propionates; C013200:pinoresinol diglucoside; D005947:Glucose; C103298:pinoresinol