Preparation, characterization and antioxidant activities of acetylated polysaccharides from Cyclocarya paliurus leaves.
In this study, polysaccharides extracted from Cyclocarya paliurus leaves were modified to obtain its three acetylated derivatives, Ac-CP1, Ac-CP2, and Ac-CP3. The physicochemical characteristics and antioxidant activities of acetylated derivatives were investigated. The results of chemical and FT-IR spectrum analysis showed differences between acetylated derivatives and native C. paliurus polysaccharide, which revealed that the acetylation were successful. Relative to unmodified polysaccharide, the protein contents of acetylated derivatives decreased, while carbohydrate values increased. The molecular weight (Mw) of acetylated derivatives were approximately 1.05-1.09×10(6)Da and were mainly composed of Ara, Gal, Glc, Man, GalA. Ac-CP1 with relatively low degree of substitution (0.13±0.01) exhibited excellent antioxidant activity in DPPH radical assay (95.21±0.89%), and also had strong chelating activity on β-carotene-linoleic acid assay (34.64±2.07%) at 0.5mg/ml. In addition, scanning electron microscope (SEM) observations suggested that acetylation could change the morphology and structure of polysaccharides from C. paliurus leaves.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: jhxie@ncu.edu.cn.
Jian-Hua
JH
Xie
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Fan
F
Zhang
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Zhi-Jun
ZJ
Wang
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Ming-Yue
MY
Shen
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Shao-Ping
SP
Nie
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: myxie@ncu.edu.cn.
Ming-Yong
MY
Xie
D000107:Acetylation; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D029525:Juglandaceae / Q000737:chemistry*; D019787:Linoleic Acid / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D019207:beta Carotene / Q000737:chemistry
D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D011134:Polysaccharides; D019207:beta Carotene; D019787:Linoleic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl
Acetylation; Antioxidant activity; Characterization; Cyclocarya paliurus; Physicochemical; Polysaccharide