24-Epibrassinoslide enhances plant tolerance to stress from low temperatures and poor light intensities in tomato (Lycopersicon esculentum Mill.).
Brassinosteroids (Brs) are a newly recognized group of active steroidal hormones that occur at low concentrations in all plant parts and one of the active and stable forms is 24-epibrassinolide (EBR). We investigated the effect of EBR on tomato (Lycopersicon esculentum Mill.) and its mechanism when seedlings were exposed to low temperature and poor light stress conditions. Leaves of stress-tolerant 'Zhongza9' and stress-sensitive 'Zhongshu4' cultivars were pre-treated with spray solutions containing either 0.1 μM EBR or no EBR (control). The plants were then transferred to chambers where they were exposed to low temperatures of 12 °C/6 °C (day/night) under a low light (LL) level of 80 μmol · m(-2) · s(-1). Exogenous application of EBR significantly increased the antioxidant activity of superoxide dismutase, catalase and peroxidase, and decreased the rate of O2 · (-) formation and H2O2 and malondialdehyde contents. Additionally, the ATP synthase β subunit content was increased by exogenous hormone application. Based on these results, we conclude that exogenous EBR can elicit synergism between the antioxidant enzyme systems and the ATP synthase β subunit so that scavenging of reactive oxygen species becomes more efficient. These activities enable plants to cope better under combined low temperature and poor light stresses.
College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
Lirong
L
Cui
College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China. zouzhirong2005@hotmail.com.
Zhirong
Z
Zou
College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
Jing
J
Zhang
College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
Yanyan
Y
Zhao
College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
Fei
F
Yan
D000064:Acclimatization / Q000187:drug effects*; D000975:Antioxidants / Q000494:pharmacology*; D060406:Brassinosteroids / Q000737:chemistry / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D058639:Cold-Shock Response / Q000187:drug effects*; D018551:Solanum lycopersicum / Q000187:drug effects* / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D013260:Steroids, Heterocyclic / Q000737:chemistry / Q000494:pharmacology*; D013472:Sunlight; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D060406:Brassinosteroids; D013260:Steroids, Heterocyclic; D008315:Malondialdehyde; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D025261:Mitochondrial Proton-Translocating ATPases; D010100:Oxygen; C023623:brassinolide
24-Epibrassinolide; ATP synthase β subunit; Antioxidant enzymes; Mass spectrometry; Reactive oxygen species; Tomato