Polythiophene-g-poly(ethylene glycol) with Lateral Amino Groups as a Novel Matrix for Biosensor Construction.
In the ever-expanding field of conducting polymer research, functionalized graft hybrid copolymers have gained considerable interest in the biomedical engineering and biosensing applications, particularly. In the present work, a new biosensor based on conducting graft copolymer for the detection of phenolic compounds was developed. Thereby, a robust and novel material, namely "polythiophene-g-poly(ethylene glycol) with lateral amino groups" (PT-NH2-g-PEG) hybrid conducting polymer was synthesized via Suzuki condensation polymerization and characterized with (1)H NMR analysis, UV-vis spectroscopy, gel permeation chromatography (GPC) and fluorescence spectroscopy. PT-NH2-g-PEG architecture was then applied as an immobilization matrix to accomplish extended biosensing function. In a typical process, Laccase was utilized as a model enzyme for the detection of phenolic compounds. Detailed surface characterization of PT-NH2-g-PEG/Lac was performed by cyclic voltammetry, electrochemical impedance spectroscopy, atomic force microscopy, fluorescence microscopy and scanning electron microscopy measurements. Optimum pH and polymer amount were found to be pH 6.5 and 0.5 mg polymer, respectively, with the linear range of 0.0025-0.05 mM and 132.45 μA/mM sensitivity. The kinetic parameters of PT-NH2-g-PEG/Lac are 0.026 mM for Km(app) and 7.38 μA for Imax, respectively. Furthermore, the PT-NH2-g-PEG/Lac biofilm was retained 82% of its activity for 12 days indicating excellent recovery as tested with artificial wastewater.
Istanbul Technical University , Department of Chemistry, Faculty of Science and Letters, Istanbul, Turkey.
Huseyin
H
Akbulut
Ege University Faculty of Science Biochemistry Department 35100 Bornova-Izmir, Turkey.
Guliz
G
Bozokalfa
Ege University Faculty of Science Biochemistry Department 35100 Bornova-Izmir, Turkey.
Duygu N
DN
Asker
Ege University Faculty of Science Biochemistry Department 35100 Bornova-Izmir, Turkey.
Bilal
B
Demir
Ege University Faculty of Science Biochemistry Department 35100 Bornova-Izmir, Turkey.|Ege University , Institute of Drug Abuse Toxicology & Pharmaceutical Sciences, 35100 Bornova, Izmir, Turkey.
Emine
E
Guler
Ege University Faculty of Science Biochemistry Department 35100 Bornova-Izmir, Turkey.
Dilek
D
Odaci Demirkol
Ege University Faculty of Science Biochemistry Department 35100 Bornova-Izmir, Turkey.|Ege University , Institute of Drug Abuse Toxicology & Pharmaceutical Sciences, 35100 Bornova, Izmir, Turkey.
Suna
S
Timur
Istanbul Technical University , Department of Chemistry, Faculty of Science and Letters, Istanbul, Turkey.|Center of Excellence for Advanced Materials Research (CEAMR) and Chemistry Department Faculty of Science, King Abdulaziz University , P.O. Box 80203, Jeddah 21589, Saudi Arabia.
Yusuf
Y
Yagci
D000588:Amines / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D004563:Electrochemistry; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000378:metabolism; D018625:Microscopy, Atomic Force; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D013499:Surface Properties; D013876:Thiophenes / Q000138:chemical synthesis / Q000737:chemistry*; D017971:Tin Compounds / Q000737:chemistry
D000588:Amines; D013876:Thiophenes; D017971:Tin Compounds; D011092:Polyethylene Glycols; C109984:indium tin oxide; D006108:Graphite; D042845:Laccase
biomolecule immobilization; biosensor; conducting polymers; graft copolymer; poly(ethylene glycol); polythiophene; surface modification