The genetic polymorphism in the STK11 does not affect gestational diabetes.
Gestational diabetes mellitus (GDM) is defined as carbohydrate intolerance of variable severity that develops during pregnancy. Recent studies indicate that GDM onset is rapid, and that women with GDM will develop other metabolic disorders such as obesity, type 2 diabetes, and cardiovascular disease in their future. Serine/threonine kinase 11 (STK11) is engaged in the insulin signaling pathway and encoded protein is an important activator of adenosine monophosphate activated protein kinase. Based on the previously reported association between the STK11 gene and diabetes, we aimed to investigate whether the rs8111699 polymorphism in STK11 has any role in gestation diabetes in Saudi women. In this case-control study, we recruited pregnant Saudi women based on biochemical analysis of their blood samples. Genomic DNA was obtained from confirmed subjects (200 GDM cases and 300 non-GDM). PCR-RFLP analysis was performed to detect the C528G polymorphism in the STK11 gene. The anthropometric and clinical data were similar between the GDM and non-GDM subjects (p > 0.05), whereas the biochemical analysis was significantly different between the cases and controls (p < 0.05). The genotype and allele frequencies between of the STK11 gene were not statistically significant difference between the GDM and non-GDM groups (OR=0.82; 95% CI:=0.6-1.0; p=0.12). Our study suggests that the rs8111699 polymorphism has no role in the development of GDM in pregnant Saudi women.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh-11433, Kingdom of Saudi Arabia.
Khalid Khalaf
KK
Alharbi
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh-11433, Kingdom of Saudi Arabia.
Imran Ali
IA
Khan
College of Dentistry, King Saud University, Riyadh-11545, Kingdom of Saudi Arabia.
Malek H
MH
Eldesouky
Department of Obstetrics and Gynecology, College of Medicine, King Khalid University Hospital, Riyadh-11555, Kingdom of Saudi Arabia.
Malak Mohammed
MM
Al-Hakeem
Department of Obstetrics and Gynecology, College of Medicine, King Khalid University Hospital, Riyadh-11555, Kingdom of Saudi Arabia.
Zeinab
Z
Abotalib
D000091162:AMP-Activated Protein Kinase Kinases; D000328:Adult; D000483:Alleles; D000886:Anthropometry; D016022:Case-Control Studies; D016640:Diabetes, Gestational / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D055815:Young Adult
D007328:Insulin; D017346:Protein Serine-Threonine Kinases; C109790:STK11 protein, human; D000091162:AMP-Activated Protein Kinase Kinases