Reference proteome of highly purified human Th1 cells reveals strong effects on metabolism and protein ubiquitination upon differentiation.
The differentiation of human CD4(+) T cells into T helper cell subtypes and regulatory T cells is crucial to the immune response. Among subtypes, Th1 cells are dominant, representing approximately 50% of all lymphocytes. Thus far, most global proteomic studies have used only partially purified T helper cell subpopulations and/or have employed artificial protocols for inducing specific T helper cell subtypes and/or used gel-based approaches. These studies have shed light on molecular details of certain aspects of the proteome; nevertheless a global analysis of high purity primary naïve and Th1 cells by LC-MS/MS is required to provide a reference dataset for proteome-based T cell subtype characterization. The utilization of highly purified Th1 cells for a global proteome assessment and the bioinformatic comparison to naïve cells reveals changes in cell metabolism and the ubiquitination pathway upon T cell differentiation. All MS data have been deposited in the ProteomeXchange with identifier PXD001066 (http://proteomecentral.proteomexchange.org/dataset/PXD001066).
Integrative Biology Program, INGM - National Institute for Molecular Genetics, Milan, Italy.
Massimiliano
M
Pagani
Department of Proteomics, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.
Maxie
M
Rockstroh
Department of Proteomics, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.
Maj
M
Schuster
Integrative Biology Program, INGM - National Institute for Molecular Genetics, Milan, Italy.
Grazisa
G
Rossetti
Integrative Biology Program, INGM - National Institute for Molecular Genetics, Milan, Italy.
Monica
M
Moro
Integrative Biology Program, INGM - National Institute for Molecular Genetics, Milan, Italy.
Mariacristina
M
Crosti
Department of Proteomics, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.
Janina M
JM
Tomm
D002454:Cell Differentiation; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D006801:Humans; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D018417:Th1 Cells / Q000166:cytology / Q000378:metabolism*; D054875:Ubiquitination
D020543:Proteome
Cell biology; Human T-lymphocytes; Immunology; LC-MS/MS; Proteome reference map; Th1-cells