Antinociceptive and Anti-Inflammatory Effects of Total Alkaloid Extract from Fumaria capreolata.
Fumaria capreolata is used in traditional medicine in North Africa for its gastrointestinal and anti-inflammatory activities. The present study investigates the effects of total alkaloids extracted from the aerial parts of Fumaria capreolata (AFC) on LPS-induced production of proinflammatory mediators (IL-6, IL-1β, iNOS, TNF-α, COX-2, and MIP-2) in RAW264.7 cells. AFC significantly reduced the inflammatory response inhibiting the production of nitric oxide (NO) and IL-6 in a dose-dependent manner, without affecting the viability of cells, and downregulated mRNA expression of proinflammatory key players: IL-6, IL-1β, iNOS, TNF-α, and COX-2. AFC antinociceptive and anti-inflammatory properties were also evaluated on the acetic acid- and formalin-induced pain models in mice. AFC oral administration significantly inhibited acetic acid-induced writhes and reduced formalin-induced paw licking time. Therefore, AFC may be a potential candidate for the treatment of inflammatory diseases, such as colitis and arthritis.
CIBER-EHD, Department of Pharmacology, ibs. GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento, S/N, Armilla, 18100 Granada, Spain ; Laboratory of Plant Biotechnology and Ethnobotany, Faculty of Natural Sciences and Life, University of Bejaia, 06000 Bejaia, Algeria.
Noureddine
N
Bribi
CIBER-EHD, Department of Pharmacology, ibs. GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento, S/N, Armilla, 18100 Granada, Spain.
Francesca
F
Algieri
CIBER-EHD, Department of Pharmacology, ibs. GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento, S/N, Armilla, 18100 Granada, Spain.
Alba
A
Rodriguez-Nogales
CIBER-EHD, Department of Pharmacology, ibs. GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento, S/N, Armilla, 18100 Granada, Spain.
Jose
J
Garrido-Mesa
CIBER-EHD, Department of Pharmacology, ibs. GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento, S/N, Armilla, 18100 Granada, Spain.
Teresa
T
Vezza
Laboratory of Plant Biotechnology and Ethnobotany, Faculty of Natural Sciences and Life, University of Bejaia, 06000 Bejaia, Algeria.
Fadila
F
Maiza
CIBER-EHD, Department of Pharmacology, ibs. GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento, S/N, Armilla, 18100 Granada, Spain.
Maria Pilar
MP
Utrilla
CIBER-EHD, Department of Pharmacology, ibs. GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento, S/N, Armilla, 18100 Granada, Spain.
Maria Elena
ME
Rodriguez-Cabezas
CIBER-EHD, Department of Pharmacology, ibs. GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento, S/N, Armilla, 18100 Granada, Spain.
Julio
J
Galvez