Efficient separation of tanshinones by polyvinylpyrrolidone-stabilized graphene-modified micellar electrokinetic chromatography.
In this work, a PVP-stabilized graphene was used in MEKC for the separation of tanshinones. Seven structurally similar tanshinones were studied, that is, tanshinone IIB, dihydrotanshinone I, tanshinone I, cryptotanshinone, 1,2-dihydrotanshinone I, miltirone, and tanshinone IIA. To achieve optimal conditions, graphene concentration, sample solvent composition, SDS concentration, 2-propanolconcentration, and buffer pH were investigated. At a separation voltage of 30 kV and a 41.5 cm effective length fused-silica capillary, good resolution within 12 min was performed using 10 mM borate buffer (pH 9.3) containing 30 mM SDS, 10% v/v 2-propanol and 6 μg/mL graphene. The method was validated in terms of linearity (r(2) > 0.9970), intra- and inter-day precision were less than 3.56 and 4.83%, respectively. The proposed method was then successfully applied to Danshentong capsule, an herbal preparation from Salvia miltiorrhiza. Our results indicated the high separation efficiency of PVP-stabilized graphene provided new opportunities for the analysis of complex samples.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, P. R. China.
Hua
H
Yang
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, P. R. China.
Yao
Y
Ding
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, P. R. China.
Wen
W
Gao
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, P. R. China.
Lian-Wen
LW
Qi
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, P. R. China.
Jun
J
Cao
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, P. R. China.
Ping
P
Li
D019840:2-Propanol / Q000737:chemistry; D045784:Abietanes / Q000737:chemistry / Q000302:isolation & purification*; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D005663:Furans; D006108:Graphite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010616:Phenanthrenes / Q000737:chemistry / Q000302:isolation & purification; D011205:Povidone / Q000737:chemistry; D011809:Quinones; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry
D045784:Abietanes; D002021:Buffers; D004365:Drugs, Chinese Herbal; D005663:Furans; D010616:Phenanthrenes; D011809:Quinones; C021751:tanshinone; C068880:miltirone; C000713095:dihydrotanshinone I; C037886:cryptotanshinone; D006108:Graphite; D011205:Povidone; D019840:2-Propanol
EKC; Graphene; PVP; Pseudostationary phase; Tanshinone