A new coumarin isolated from Sarcandra glabra as potential anti-inflammatory agent.
One new coumarin, 3,5-dihydroxy-7-O-α-L-rhamno pyranosyl-2H-chromen-2-one (1), was isolated from the whole plant of Sarcandra glabra. The structure was elucidated by spectroscopic methods. Our results indicated that 1 significantly inhibit nitric oxide (NO) production in LPS-induced RAW264.7 macrophages. RT-PCR analysis indicated it inhibited iNOS mRNA expression. In addition, Western blot analysis showed that 1 attenuated LPS-induced synthesis of iNOS protein in the macrophages. These results suggest that 1 could be potential anti-inflammatory agent by down-regulating iNOS expression.
a Second Affiliated Hospital , Guangzhou University of Chinese Medicine , Guangzhou , China.
Maojie
M
Wang
a Second Affiliated Hospital , Guangzhou University of Chinese Medicine , Guangzhou , China.
Jin
J
Zhao
a Second Affiliated Hospital , Guangzhou University of Chinese Medicine , Guangzhou , China.|b The Postdoctoral Research Station , Guangzhou University of Chinese Medicine , Guangzhou , China.
Ya
Y
Zhao
a Second Affiliated Hospital , Guangzhou University of Chinese Medicine , Guangzhou , China.
Run-Yue
RY
Huang
c School of Chinese Pharmaceutical Science , Guangzhou University of Chinese Medicine , Guangzhou , China.
Geng
G
Li
a Second Affiliated Hospital , Guangzhou University of Chinese Medicine , Guangzhou , China.
Xing
X
Zeng
a Second Affiliated Hospital , Guangzhou University of Chinese Medicine , Guangzhou , China.
Xiong
X
Li
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification; D002460:Cell Line; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism; D019684:Magnoliopsida / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D000067996:RAW 264.7 Cells
D000893:Anti-Inflammatory Agents; D003374:Coumarins; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009569:Nitric Oxide; C030123:coumarin; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II
NO; RAW264.7; Sarcandra glabra; coumarin; iNOS