Comparative analysis of eukaryotic cell-free expression systems.
Cell-free protein synthesis (CFPS) allows researchers to rapidly generate functional proteins independent of cell culture. Although advances in eukaryotic lysates have increased the amount of protein that can be produced, the nuances of different translation systems lead to variability in protein production. To help overcome this problem, we have compared the relative yield and template requirements for three commonly used commercial cell-free translation systems: wheat germ extract (WGE), rabbit reticulocyte lysate (RRL), and HeLa cell lysate (HCL). Our results provide a general guide for researchers interested in using cell-free translation to generate recombinant protein for biomedical applications.
Center for Personalized Medicine, Arizona State University, Tempe, AZ.|School of Life Sciences, Arizona State University, Tempe, AZ.
Emily M
EM
Hartsough
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ.
Pankti
P
Shah
Center for Personalized Medicine, Arizona State University, Tempe, AZ.
Andrew C
AC
Larsen
Center for Personalized Medicine, Arizona State University, Tempe, AZ.|Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ.
John C
JC
Chaput
D020121:5' Untranslated Regions; D000818:Animals; D002474:Cell-Free System; D006367:HeLa Cells; D006801:Humans; D000067556:Internal Ribosome Entry Sites; D010936:Plant Extracts / Q000378:metabolism; D014176:Protein Biosynthesis; D015202:Protein Engineering / Q000379:methods*; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D012156:Reticulocytes; D014908:Triticum
D020121:5' Untranslated Regions; D000067556:Internal Ribosome Entry Sites; D010936:Plant Extracts; D011994:Recombinant Proteins
cell-free translation; recombinant protein synthesis; translation enhancing elements