Immunomodulatory activity of purified arabinoxylans from finger millet (Eleusine coracana, v. Indaf 15) bran.
Biological activities of alkali extracted (Barium hydroxide: BE-480 kDa, Potassium hydroxide: KE1-1080 and KE2-40 kDa), purified arabinoxylans (AX) from the finger millet bran varying in their molecular weight, phenolic acid content, arabinose to xylose ratios were evaluated for their immune-stimulatory activities using murine lymphocytes and peritoneal exudate macrophages. All three purified AX displayed significant (p < 0.001) mitogenic activity and activation of macrophages including phagocytosis. Among these BE has shown higher enhancing lymphocyte proliferation (>2 fold) and macrophage phagocytosis than KE1 and KE2. The above results clearly documented that the immunostimulatory activity of arabinoxylans is directly proportional to the amount of ferulic acid content (0.11 mg/100 g), whereas molecular weight as well as arabinose/xylose ratio, did not have any bearing. Purified AX from the finger millet bran can be explored as a potent natural immunomodulator.
Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, Mysore, 570 020 Karnataka India.
M R
MR
Savitha Prashanth
Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, Mysore, 570 020 Karnataka India.
R R
RR
Shruthi
Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, Mysore, 570 020 Karnataka India.
G
G
Muralikrishna
Arabinoxylan; Finger millet bran; Immunomodulation; Macrophage activation; Mitogenic activity; Phagocytosis