The effect of temperature on apoptosis and adipogenesis on skeletal muscle satellite cells derived from different muscle types.
Satellite cells are multipotential stem cells that mediate postnatal muscle growth and respond differently to temperature based upon aerobic versus anaerobic fiber-type origin. The objective of this study was to determine how temperatures below and above the control, 38°C, affect the fate of satellite cells isolated from the anaerobic pectoralis major (p. major) or mixed fiber biceps femoris (b. femoris). At all sampling times, p. major and b. femoris cells accumulated less lipid when incubated at low temperatures and more lipid at elevated temperatures compared to the control. Satellite cells isolated from the p. major were more sensitive to temperature as they accumulated more lipid at elevated temperatures compared to b. femoris cells. Expression of adipogenic genes, CCAAT/enhancer-binding protein β (C/EBPβ) and proliferator-activated receptor gamma (PPARγ) were different within satellite cells isolated from the p. major or b. femoris. At 72 h of proliferation, C/EBPβ expression increased with increasing temperature in both cell types, while PPARγ expression decreased with increasing temperature in p. major satellite cells. At 48 h of differentiation, both C/EBPβ and PPARγ expression increased in the p. major and decreased in the b. femoris, with increasing temperature. Flow cytometry measured apoptotic markers for early apoptosis (Annexin-V-PE) or late apoptosis (7-AAD), showing less than 1% of apoptotic satellite cells throughout all experimental conditions, therefore, apoptosis was considered biologically not significant. The results support that anaerobic p. major satellite cells are more predisposed to adipogenic conversion than aerobic b. femoris cells when thermally challenged.
Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio.
Rachel L
RL
Harding
Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio.
Daniel L
DL
Clark
Department of Animal Sciences, The Hebrew University of Jerusalem, Rehovot, Israel.
Orna
O
Halevy
Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio.
Cynthia S
CS
Coy
Institute of Animal Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
Shlomo
S
Yahav
Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio velleman.1@osu.edu.
Sandra G
SG
Velleman
Adipogenesis; apoptosis; fiber type; muscle; myogenic satellite cell; temperature