A Dual-Response Fluorescent Probe Reveals the H2 O2 -Induced H2 S Biogenesis through a Cystathionine β-Synthase Pathway.
The two signaling molecules H2 S and H2 O2 play key roles in maintaining intracellular redox homeostasis. The biological relationship between H2 O2 and H2 S remains largely unknown in redox biology. In this study, we rationally designed and synthesized single- and dual-response fluorescent probes for detecting both H2 O2 and H2 S in living cells. The dual-response probe was shown to be capable of mono- and dual-detection of H2 O2 and H2 S selectively and sensitively. Detailed bioimaging studies based on the probes revealed that both exogenous and endogenous H2 O2 could induce H2 S biogenesis in living cells. By using gene-knockdown techniques with bioimaging, the H2 S biogenesis was found to be majorly cystathionine β-synthase (CBS)-dependent. Our finding shows the first direct evidence on the biological communication between H2 O2 (ROS) and H2 S (RSS) in vivo.
State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Engineering (BUCT), 15 Beisanhuan East Road, Chaoyang District, Beijing 100029 (P. R. China). yilong@mail.buct.edu.cn.
Long
L
Yi
Department of Chemical Biology, State Key Laboratory of Elemento-Organic Chemistry, National Engineering Research Center of Pesticide (Tianjin), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Weijin Road 94, Tianjin 300071 (P. R. China).
Lv
L
Wei
Department of Chemical Biology, State Key Laboratory of Elemento-Organic Chemistry, National Engineering Research Center of Pesticide (Tianjin), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Weijin Road 94, Tianjin 300071 (P. R. China).
Runyu
R
Wang
State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Engineering (BUCT), 15 Beisanhuan East Road, Chaoyang District, Beijing 100029 (P. R. China).
Changyu
C
Zhang
State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Engineering (BUCT), 15 Beisanhuan East Road, Chaoyang District, Beijing 100029 (P. R. China).
Jie
J
Zhang
State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Engineering (BUCT), 15 Beisanhuan East Road, Chaoyang District, Beijing 100029 (P. R. China). twtan@mail.buct.edu.cn.
Tianwei
T
Tan
Department of Chemical Biology, State Key Laboratory of Elemento-Organic Chemistry, National Engineering Research Center of Pesticide (Tianjin), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Weijin Road 94, Tianjin 300071 (P. R. China). zhenxi@nankai.edu.cn.
Zhen
Z
Xi
D001686:Biological Phenomena; D003541:Cystathionine beta-Synthase / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry* / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000737:chemistry* / Q000378:metabolism
D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide; D003541:Cystathionine beta-Synthase; D006862:Hydrogen Sulfide
fluorescent imaging; fluorescent probes; hydrogen peroxide; hydrogen sulfide; redox homeostasis