Aspergillus fumigatus diffusates suppress polymorphonuclear neutrophil phagocytic functions and respiratory burst levels in hematopoietic stem cell transplantation patients.
Invasive aspergillosis (IA) is a severe infection that commonly occurs in immunocompromised patients after hematopoietic stem cell transplantation (HSCT). The present study explores the effect of Aspergillus fumigatus diffusates (AfDs) on phagocytic function and superoxide anion (O2(-)) burst levels in polymorphonuclear neutrophils (PMNs) from post-HSCT patients. A. fumigatus conidia with or without AfD were used to stimulate the PMN from healthy donor or HSCT patient for two hours. PMN morphology was visualized by scanning electron microscopy. The levels of respiratory burst O2(-) produced by the PMNs were determined by flow cytometry. PMN phagocytic rates and phagocytic indexes were observed and calculated using periodic acid-Schiff (PAS) staining under a light-field microscope. No difference was found between the PMN phagocytic rates, phagocytic indexes, or O2(-) respiratory burst levels in health donor PMNs following treatments of A. fumigatus conidia with or without AfD. However, significant inhibition of these indices was seen in the PMNs from HSCT patients following treatment of A. fumigatus conidia plus AfD, compared to that with conidium treatment alone (P < 0.05). Therefore, AfD significantly inhibited the phagocytic function of PMNs from HSCT patients, potentially through inhibition of intracellular respiratory burst levels during phagocytosis. This suggests that the reason underlying the greater susceptibility of HSCT patients to aspergillosis might be the existence of AfD in vivo during infection. Further research on the mechanisms by which AfD affects the phagocytic function of PMNs from HSCT patients is therefore of great significance for the prevention of IA.
Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing, China.
X H
XH
Chen
Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing, China.
Y C
YC
Deng
Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing, China.
B Y
BY
Zhong
Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing, China FeiHao92001@163.com.
F
F
Hao
D000328:Adult; D001228:Aspergillosis / Q000150:complications / Q000276:immunology / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006801:Humans; D056747:Immunomodulation; D008297:Male; D009504:Neutrophils / Q000382:microbiology* / Q000473:pathology / Q000502:physiology* / Q000648:ultrastructure; D009894:Opportunistic Infections; D010587:Phagocytosis / Q000276:immunology*; D016897:Respiratory Burst / Q000276:immunology*