One-step preparation of magnetic imprinted nanoparticles adopting dopamine-cupric ion as a co-monomer for the specific recognition of bovine hemoglobin.
A novel magnetic core-shell polydopamine-cupric ion complex imprinted polymer was prepared in one-step through surface imprinting technology, which could specifically recognize bovine hemoglobin from the real blood samples. The polymerization conditions and adsorption performance of the resultant nanomaterials were investigated in detail. The results showed that the cupric ion played an important role in the recognition of template proteins. The saturating adsorption capacity of this kind of imprinted polymers was 2.23 times greater than those of imprinted polymers without cupric ion. The imprinting factor of the imprinted materials was as high as 4.23 for the template molecule. The selective separation bovine hemoglobin from the real blood sample is successfully applied. In addition, the prepared materials had excellent stability and no obvious deterioration after five adsorption-regeneration cycles. Easy preparation, rapid separation, high binding capacity and satisfactory selectivity for the template protein make this polymer attractive in the separation of high-abundance proteins.
Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.
Ruixia
R
Gao
Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.|College of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
Lili
L
Zhang
Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.|College of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
Yi
Y
Hao
Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.|College of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
Xihui
X
Cui
Department of Hepatobiliary Surgery, First Hospital of Xi'an Jiaotong University, Xi'an, China.
Dechun
D
Liu
College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai, China.
Min
M
Zhang
Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.|College of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
Yuhai
Y
Tang
D000327:Adsorption; D000818:Animals; D002417:Cattle; D003300:Copper / Q000737:chemistry*; D004298:Dopamine / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis* / Q000737:chemistry; D007211:Indoles / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*
D006454:Hemoglobins; D007211:Indoles; D007477:Ions; D058185:Magnetite Nanoparticles; D011108:Polymers; C568283:polydopamine; D003300:Copper; D004298:Dopamine
Metal coordination; Polydopamine; Proteins; Surface imprinting