Integrated microRNA-mRNA analysis of pancreatic ductal adenocarcinoma.
The main aim of this study was to explore the underlying molecular mechanisms and potential target molecules of pancreatic adenocarcinoma. The miRNA (GSE32678) and mRNA (GSE32676) expression profiles of patients with pancreatic ductal adenocarcinoma and healthy controls were downloaded from the Gene Expression Omnibus database. Differentially expressed miRNA and differentially expressed genes were identified by analyzing the microarray algorithm after data preprocessing. Functional analysis was conducted by the Database for Annotation, Visualization and Integrated Analysis. miRNA-mRNA regulation pairs were obtained in TarMir database. The node degree of hsa-miR-200c, hsa-miR-429, and hsa-miR-200b (miRNA), and EFNB2, MYRIP, and PHF17 (mRNA) were extremely high in the miRNA-mRNA network, indicating that these miRNA and mRNA may play a key role in the development of pancreatic cancer. Our study screened out some target miRNAs and mRNAs for pancreatic ductal adenocarcinoma, which may be helpful in its diagnosis and treatment.
Key Laboratory of Cancer Prevention and Therapy, Department of Lymphoma, Sino-US Center of Lymphoma and Leukemia, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, China.
P F
PF
Liu
Department of Radiotherapy, Second Hospital of Tianjin Medical University, Tianjin, China.
W H
WH
Jiang
Department of Cell Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
Y T
YT
Han
Shanxi Agricultural University, Shanxi, China.
L F
LF
He
Key Laboratory of Cancer Prevention and Therapy, Department of Lymphoma, Sino-US Center of Lymphoma and Leukemia, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, China.
H L
HL
Zhang
Department of Gastrointestinal Surgery, Inner Mongolia People's Hospital, Hohhot, China snowlpf@163.com.
H
H
Ren
D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010190:Pancreatic Neoplasms / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012015:Reference Standards
D035683:MicroRNAs; D012333:RNA, Messenger