Fluorescent In Situ Hybridization of Nuclear Bodies in Drosophila melanogaster Ovaries.
Fluorescent in situ hybridization (FISH) is a technique for determining the cytological localization of RNA or DNA molecules. There are many approaches available for generating in situ hybridization probes and conducting the subsequent hybridization steps. Here, we describe a simple and reliable FISH method to label small RNAs (200-500 nucleotides in length) that are enriched in nuclear bodies in Drosophila melanogaster ovaries, such as Cajal bodies (CBs) and histone locus bodies (HLBs). This technique can also be applied to other Drosophila tissues, and to abundant mRNAs such as histone transcripts.
Department of Embryology, Carnegie Institution for Science, Baltimore, MD, 21218, USA.
Zehra F
ZF
Nizami
Ji-Long
JL
Liu
Joseph G
JG
Gall
D000818:Animals; D020541:Coiled Bodies / Q000235:genetics / Q000648:ultrastructure*; D004331:Drosophila melanogaster; D004625:Embryo, Nonmammalian; D005260:Female; D006657:Histones / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D010053:Ovary / Q000254:growth & development / Q000648:ultrastructure*; D012333:RNA, Messenger / Q000235:genetics*
D006657:Histones; D012333:RNA, Messenger