A new methodological approach to estimation of IgA1 and IgA2 content in human serum using recombinant IgA1 protease from Neisseria meningitidis.
OBJECTIVE
A new approach to estimation of IgA subclass levels and IgA1/IgA2 ratio using enzymatically active and inactive forms of Neisseria meningitidis IgA1 protease was developed.


RESULTS
The approach was tested using the sera of healthy volunteers and patients with meningococcal meningitis. There was a significant increase in the IgA1 level in patients with meningitis (mean titer 1:1546 ± 352) compared to healthy volunteers (mean titer 1:546 ± 282), while the IgA2 content remained unchanged. The IgA1/IgA2 ratio was 6.3 for the healthy volunteers and 12.8 for patients with meningitis. IgA2 for the patients with meningitis and the healthy volunteers were almost unchanged, 1:86 ± 61 and 1:121 ± 46, respectively.


CONCLUSIONS
The proposed method is economical and reliable and can be used for evaluation of IgA1 and IgA2 in clinical laboratories or for research purposes.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, Moscow, 117997, Russia. zhigis@ibch.ru.
Larisa S
http://orcid.org/0000-0001-8296-5110
LS
Zhigis
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, Moscow, 117997, Russia.
Olga V
OV
Kotel'nikova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, Moscow, 117997, Russia.
Alexander A
AA
Vikhrov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, Moscow, 117997, Russia.
Aleksey A
AA
Zinchenko
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, Moscow, 117997, Russia.
Oksana V
OV
Serova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, Moscow, 117997, Russia.
Vera S
VS
Zueva
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, Moscow, 117997, Russia.
Olga A
OA
Razgulyaeva
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, Moscow, 117997, Russia.
Elena A
EA
Gordeeva
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, Moscow, 117997, Russia.
Tatiana D
TD
Melikhova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, Moscow, 117997, Russia.
Elena A
EA
Nokel
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, Moscow, 117997, Russia.
Alexander P
AP
Alliluev
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, Moscow, 117997, Russia.
Elena Yu
EY
Drozhzhina
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, Moscow, 117997, Russia.
Lev D
LD
Rumsh
D001426:Bacterial Proteins / Q000378:metabolism; D001774:Blood Chemical Analysis; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood* / Q000378:metabolism; D009345:Neisseria meningitidis / Q000201:enzymology; D011994:Recombinant Proteins / Q000378:metabolism; D012680:Sensitivity and Specificity; D012697:Serine Endopeptidases / Q000378:metabolism*
D001426:Bacterial Proteins; D007070:Immunoglobulin A; D011994:Recombinant Proteins; D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase
Immunoglobulin A; Meningococcus; Neisseria meningitidis; Protease; Recombinant IgA1 protease