Effects of Moderate Alcohol Intake in the Bladder of the Otsuka Long Evans Tokushima Fatty Diabetic Rats.
Diabetes is related with a number of cystopathic complications. However, there have been no studies about the influence of alcohol consumption in the bladder of type 2 diabetes. Thus, we investigated the effect of moderate alcohol intake in the bladder of the Otsuka Long Evans Tokushima Fatty (OLETF) diabetic rat. The non-diabetic Long-Evans Tokushima Otsuka (LETO, n=14) and the OLETF control group (n=14) were fed an isocaloric diet; the LETO (n=14) and the OLETF ethanol group (n=14) were fed 36% ethanol 7 g/kg/day. After ten weeks, muscarinic receptors, RhoGEFs, myogenic change, and the level of oxidative stress were evaluated. Moderate alcohol intake significantly decreased excessive muscarinic receptor and Rho kinase expressions in the OLETF rats compared with the LETO rats. In addition, iNOS and collagen expression were not changed in the OLETF rats in spite of alcohol consumption. Superoxide dismutase levels, which is involved in antioxidant defense, in the LETO rats were significantly decreased after alcohol consumption, however those in the OLETF rats were similar. Moderate alcohol consumption reduces the oxidative stress, and may prevent molecular and pathologic changes of the bladder of rats with type 2 diabetes.
Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Woong Jin
WJ
Bae
Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Yong Sun
YS
Choi
Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Su Jin
SJ
Kim
Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Hyuk Jin
HJ
Cho
Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Sung Hoo
SH
Hong
Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Sae Woong
SW
Kim
Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Tae-Kon
TK
Hwang
Department of Psychiatry, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Dai Jin
DJ
Kim
Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea. ; Catholic Prostate Institute, The Catholic University of Korea, Seoul, Korea. ; Department of Bioinformatics, The Catholic University of Korea, Seoul, Korea.
Ji Youl
JY
Lee
D000428:Alcohol Drinking / Q000009:adverse effects; D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000378:metabolism* / Q000473:pathology; D000431:Ethanol / Q000633:toxicity*; D006801:Humans; D051381:Rats; D020334:Rats, Inbred OLETF; D017382:Reactive Oxygen Species / Q000378:metabolism*; D001743:Urinary Bladder / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology
D017382:Reactive Oxygen Species; D000431:Ethanol
Alcohols; Diabetes Mellitus; Urinary Bladder Disease