Frequent loss of the expression of multiple subunits of the SWI/SNF complex in large cell carcinoma and pleomorphic carcinoma of the lung.
The switch/sucrose non-fermenting (SWI/SNF) complex has recently emerged as a novel tumor suppressor in various human cancers. In the present study, we analyzed the expression of multiple SWI/SNF subunits in primary non-small cell lung cancer (NSCLC). A total of 133 NSCLC, consisting of 25 squamous cell carcinomas (SCC), 70 adenocarcinomas (AD), 16 large cell carcinomas (LC), and 22 pleomorphic carcinomas (PL), were immunohistochemically examined for the expression of BRG1, BRM, BAF47, ARID1A, and ARID1B. The frequency at which reductions in the expression of BRG1 were observed was significantly higher in the LC-PL group (13/38, 34.2%) than in the SCC-AD group (7/95, 7.4%). Similarly, the frequency at which reductions in the expression of BRM were observed was significantly higher in the LC-PL group (17/38, 44.7%) than in the SCC-AD group (14/95, 14.7%). The loss of the expression of ARID1A, ARID1B, and BAF47 was observed only in a fraction of NSCLC cases. Furthermore, the frequency at which the concurrent loss of multiple subunits of the SWI/SNF complex was observed was significantly higher in the LC-PL group (10/38, 26.3%) than in the SCC-AD group (8/95, 8.4%). Collectively, these results indicate that the loss of the SWI/SNF complex was related to dedifferentiation in NSCLC.
Department of Integrative Pathology, Jichi Medical University, Shimotsuke, Japan.
Taichiro
T
Yoshimoto
Department of Integrative Pathology, Jichi Medical University, Shimotsuke, Japan.|Molecular Pathology Laboratory, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Daisuke
D
Matsubara
Division of General Thoracic Surgery, Jichi Medical University, Shimotsuke, Japan.
Tomoyuki
T
Nakano
Department of Integrative Pathology, Jichi Medical University, Shimotsuke, Japan.
Tomoko
T
Tamura
Division of General Thoracic Surgery, Jichi Medical University, Shimotsuke, Japan.
Shunsuke
S
Endo
Division of Pulmonary Medicine, Jichi Medical University, Shimotsuke, Japan.
Yukihiko
Y
Sugiyama
Department of Integrative Pathology, Jichi Medical University, Shimotsuke, Japan.
Toshiro
T
Niki
D000368:Aged; D000369:Aged, 80 and over; D018287:Carcinoma, Large Cell / Q000378:metabolism* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D002467:Cell Nucleus / Q000378:metabolism* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D014157:Transcription Factors / Q000378:metabolism*
D004268:DNA-Binding Proteins; D014157:Transcription Factors
SWI/SNF complex; immunohistochemistry; lung cancer