Down-regulation of cancer/testis antigen OY-TES-1 attenuates malignant behaviors of hepatocellular carcinoma cells in vitro.
Cancer/testis (CT) antigens are normally expressed in testis and overexpressed in various tumor types. However, their biological function is largely unknown. OY-TES-1, one of cancer/testis (CT) antigens, is reported overexpression in hepatocellular carcinoma (HCC). And we assumed that OY-TES-1 contribute to oncogenesis and progression of HCC. In this study, we knocked down OY-TES-1 by small interference RNA (siRNA) in HCC cell lines (HepG2 and BEL-7404) to verify this assumption and evaluate its potential as therapeutic targets for HCC. We showed that down regulation of OY-TES-1 decreased cell growth, induced the G0/G1 arrest and apoptosis, and prevented migration and invasion in the two HCC cell lines. Further analysis revealed that down regulation of OY-TES-1 increased expression of apoptosis-regulated protein caspase-3, and decreased expression of cell cycle-regulated protein cyclin E, migration/invasion-regulated proteins MMP2 and MMP9. These findings may shed light on the gene therapy about the OY-TES-1 expression in HCC cells.
Department of Histology & Embryology, School of Pre-clinical Medicine, Guangxi Medical University China.
Jun
J
Fu
Department of Histology & Embryology, School of Pre-clinical Medicine, Guangxi Medical University China ; Key Laboratory of School of Pre-clinical Medicine, Guangxi Medical University China.
Bin
B
Luo
Department of Histology & Embryology, School of Pre-clinical Medicine, Guangxi Medical University China.
Wen-Wen
WW
Guo
Department of Histology & Embryology, School of Pre-clinical Medicine, Guangxi Medical University China.
Qing-Mei
QM
Zhang
Department of Histology & Embryology, School of Pre-clinical Medicine, Guangxi Medical University China.
Lei
L
Shi
Department of Histology & Embryology, School of Pre-clinical Medicine, Guangxi Medical University China.
Qi-Ping
QP
Hu
Department of Histology & Embryology, School of Pre-clinical Medicine, Guangxi Medical University China ; Key Laboratory of School of Pre-clinical Medicine, Guangxi Medical University China.
Fang
F
Chen
Department of Neurosurgery, First Affiliated Hospital, Guangxi Medical University China.
Shao-Wen
SW
Xiao
Department of Histology & Embryology, School of Pre-clinical Medicine, Guangxi Medical University China ; Key Laboratory of School of Pre-clinical Medicine, Guangxi Medical University China.
Xiao-Xun
XX
Xie
D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D034741:RNA, Small Interfering; D013737:Testis / Q000378:metabolism
C485651:ACRBP protein, human; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D034741:RNA, Small Interfering; D053148:Caspase 3
Cancer/testis antigen; OY-TES-1; hepatocellular carcinoma; siRNA