Sesquiterpenes and other constituents of Xylaria sp. NC1214, a fungal endophyte of the moss Hypnum sp.
Oxygenated guaiane-type sesquiterpenes, xylaguaianols A-D (1-4), an iso-cadinane-type sesquiterpene isocadinanol A (5), and an α-pyrone 9-hydroxyxylarone (6), together with five known sesquiterpenes (7-11), and four known cytochalasins (12-15) were isolated from a culture broth of Xylaria sp. NC1214, a fungal endophyte of the moss Hypnum sp. The structures of all compounds were elucidated by the analysis of their spectroscopic data and relative configurations of 1-5 were determined with the help of NMR NOESY experiments. Cytochalasins C (12), D (13), and Q (14) were investigated for their cytotoxic activity against five tumor cell lines. Cytochalasin D showed significant cytotoxicity against all five cell lines, with IC50s ranging from 0.22 to 1.44 μM, whereas cytochalasins C and Q exhibited moderate, but selective cytotoxicity.
Southwest Center for Natural Products Research and Commercialization, School of Natural Resources and the Environment, College of Agriculture and Life Sciences, The University of Arizona, 250 E. Valencia Road, Tucson, AZ 85706, United States.
Han
H
Wei
Southwest Center for Natural Products Research and Commercialization, School of Natural Resources and the Environment, College of Agriculture and Life Sciences, The University of Arizona, 250 E. Valencia Road, Tucson, AZ 85706, United States.
Ya-ming
YM
Xu
Southwest Center for Natural Products Research and Commercialization, School of Natural Resources and the Environment, College of Agriculture and Life Sciences, The University of Arizona, 250 E. Valencia Road, Tucson, AZ 85706, United States.
Patricia
P
Espinosa-Artiles
Southwest Center for Natural Products Research and Commercialization, School of Natural Resources and the Environment, College of Agriculture and Life Sciences, The University of Arizona, 250 E. Valencia Road, Tucson, AZ 85706, United States.
Manping X
MX
Liu
Southwest Center for Natural Products Research and Commercialization, School of Natural Resources and the Environment, College of Agriculture and Life Sciences, The University of Arizona, 250 E. Valencia Road, Tucson, AZ 85706, United States; State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, PR China.
Jiang-Guang
JG
Luo
School of Plant Sciences, College of Agriculture and Life Sciences, The University of Arizona, Tucson, AZ 85721, United States.
Jana M
JM
U'Ren
School of Plant Sciences, College of Agriculture and Life Sciences, The University of Arizona, Tucson, AZ 85721, United States; Department of Ecology and Evolutionary Biology, The University of Arizona, Tucson, AZ 85721, United States.
A Elizabeth
AE
Arnold
Southwest Center for Natural Products Research and Commercialization, School of Natural Resources and the Environment, College of Agriculture and Life Sciences, The University of Arizona, 250 E. Valencia Road, Tucson, AZ 85706, United States. Electronic address: leslieg1@email.arizona.edu.
A A Leslie
AA
Gunatilaka
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D044002:Bryophyta / Q000382:microbiology*; D003572:Cytochalasins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D008968:Molecular Conformation; D015394:Molecular Structure; D000081223:Polycyclic Sesquiterpenes; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification; D012717:Sesquiterpenes; D045789:Sesquiterpenes, Guaiane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013098:Xylariales / Q000737:chemistry*
D000970:Antineoplastic Agents; D003572:Cytochalasins; D000081223:Polycyclic Sesquiterpenes; D011753:Pyrones; D012717:Sesquiterpenes; D045789:Sesquiterpenes, Guaiane; C508039:cadinane; C031582:cytochalasin C
Cytochalasins; Fungal endophyte; Hypnum sp.; Sesquiterpenes; Xylaguaianols; Xylaria sp.; α-Pyrone