Serum metabolomics research of the anti-hypertensive effects of Tengfu Jiangya tablet on spontaneously hypertensive rats.
A HPLC/TOF-MS-based metabolomic study was conducted to investigate the holistic therapeutic effects of Tengfu Jiangya Tablet (TJT) on spontaneously hypertensive rats (SHRs). The SHRs were divided into valsartan (VST) group, TJT group and model group, in addition, the Wistar-Kyoto rats (WKY) were taken as normal control. Serum samples were separated and identified by HPLC/TOF-MS, while the obtained data was further processed by partial least-squares discriminant analysis (PLS-DA). A clear cluster among the four groups was observed, and we identified thirteen biomarkers involved involved in sphingolipid metabolism (sphinganine, lysosphingomyelin, ceramide), glycerophospholipid metabolism (phosphatidylcholines, phosphatidylethanolamine, lysophosphatidylcholines), arginine and proline metabolism (l-proline, citrulline), tryptophan metabolism (xanthuiulrenic acid, l-kynurenine, l-tryptophan), arachidonic acid metabolism(leukotriene D4), and linoleic acid metabolism (gamma-linolenic acid). Altered metabolic pathways involved in impaired NO production, inflammation and vascular smooth muscle cells (VSMCs) apoptosis and proliferation, which suggesting the possible pathological state in SHRs. TJT as well as VST altered the metabolic state, suggesting a possible anti-hypertension role by improving NO production, and an extra cardiovascular protection role possibly by amelioration of inflammatory state and vascular remodeling.
Experimental center of Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
Haiqiang
H
Jiang
Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China.
Zhenzhen
Z
Shen
Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China.
Yanjun
Y
Chu
Traditional Chinese Medicine Clinical Research Base for Hypertension of Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Wenhua Xi Road, Jinan 250011, China; Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China. Electronic address: yunlun.lee@hotmail.com.
Yunlun
Y
Li
Experimental center of Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
Jihua
J
Li
Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China.
Xiaoming
X
Wang
Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China.
Wenqing
W
Yang
Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China.
Xinya
X
Zhang
Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China.
Jianqing
J
Ju
Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China.
Jingwen
J
Xu
Traditional Chinese Medicine Clinical Research Base for Hypertension of Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Wenhua Xi Road, Jinan 250011, China.
Chuanhua
C
Yang
D000818:Animals; D000959:Antihypertensive Agents / Q000097:blood* / Q000627:therapeutic use; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006973:Hypertension / Q000188:drug therapy* / Q000378:metabolism; D055432:Metabolomics; D009569:Nitric Oxide / Q000096:biosynthesis; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D000959:Antihypertensive Agents; D004365:Drugs, Chinese Herbal; C000607537:tengfu jiangya; D009569:Nitric Oxide
Hypertension; Liquid chromatography; Mass spectrometry; Metabolomics; Traditional chinese medicine