Infection by Henneguya sp. (Myxozoa) in the bone tissue of the gill filaments of the Amazonian catfish Hypophthalmus marginatus (Siluriformes).
This study describes aspects of the infection caused by the myxosporean genus Henneguya, which forms cysts in the bony portion of the gill filaments of Hypophthalmusmarginatus. Specimens of this catfish were acquired dead from artisanal fishermen near the town of Cametá, state of Pará, northern Brazil, between July 2011 and May 2012. They were transported in refrigerated containers to the Carlos Azevedo Research Laboratory at the Federal Rural University of Amazonia, in Belém, where analyses were performed. After confirmation of parasitism by the genus Henneguya, observation were made using optical and differential interference contrast (DIC) microscopy. The histological technique of embedment in paraffin was used. Ziehl-Neelsen staining was applied to the histological sections. Necropsy analyses on specimens of H. marginatus showed that 80% of them (40/50) had cysts of whitish coloration inside the bony portion of the gill filaments, filled with Henneguya spores. The present study found inflammatory infiltrate in the vicinity of the cysts. Furthermore, the special Ziehl-Neelsen staining technique made it possible to mark the Henneguya sp. cysts in the bone tissue and in spore isolates in the gill tissue structure. The descriptions of these histopathological findings show that this parasite is very invasive and causes damage to its host tissues.
Laboratório de Pesquisa Carlos Azevedo, Universidade Federal Rural da Amazônia, Belém, PA, BR.
Michele
M
Velasco
Laboratório de Morfofisiologia e Sanidade Animal, Universidade do Estado do Amapá, Macapá, AP, BR.
Marcela
M
Videira
Laboratório de Pesquisa Carlos Azevedo, Universidade Federal Rural da Amazônia, Belém, PA, BR.
José Ledamir
JL
Sindeaux-Neto
Laboratório de Pesquisa Carlos Azevedo, Universidade Federal Rural da Amazônia, Belém, PA, BR.
Patrícia Dos
PD
Santos
Centro de Diagnóstico Veterinário, Presidente Prudente, SP, BR.
Osimar
O
Sanches
Laboratório de Pesquisa Edilson Matos, Universidade Federal do Pará, Belém, PA, BR.
Patrícia
P
Matos
Laboratório de Pesquisa Carlos Azevedo, Universidade Federal Rural da Amazônia, Belém, PA, BR.
Edilson
E
Matos
D000818:Animals; D001847:Bone Diseases / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D001938:Brazil; D002397:Catfishes; D005393:Fish Diseases / Q000469:parasitology* / Q000473:pathology; D005880:Gills / Q000469:parasitology*; D055764:Myxozoa; D010273:Parasitic Diseases, Animal