Separation of silver nanoparticles by hollow fiber flow field-flow fractionation: Addition of tannic acid into carrier liquid as a modifier.
A homemade hollow fiber flow-field fractionation (Hf-FlFFF) coupled with inductively coupled plasma mass spectrometry (ICP-MS) was set-up for silver nanoparticles (AgNPs) separation by using polysulfone hollow fiber membrane (30,000 MW cutoff) as a separation channel. Tannic acid and citrate stabilized AgNPs were synthesized and introduced into Hf-FlFFF. The effects of carrier liquid and stabilizing agent on retention behavior of AgNPs were investigated. Different elution behaviors were observed as follows: with 0.02% (w/v) FL-70, all of AgNPs were eluted from Hf-FlFFF but differences in retention behaviors were observed for AgNPs with tannic acid and citrate stabilizing agents; and with 30mM TRIS buffer, only tannic acid stabilized AgNPs were eluted from Hf-FlFFF, whereas citrate stabilized AgNPs were not eluted. In this work, tannic acid addition into carrier liquid was proposed to modify the surface of AgNPs and the surface of the membrane, and thereby adjusting the retention behaviors of AgNPs. Various concentrations of tannic acid were added into FL-70 and TRIS buffer. With the use of 0.1mM tannic acid in 30mM TRIS buffer as the carrier liquid, retention behaviors of both tannic acid stabilized- and citrate stabilized-AgNPs were similar and with similar fractionation recovery.
Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
Rattaporn
R
Saenmuangchin
Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
Jaruwan
J
Mettakoonpitak
Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
Juwadee
J
Shiowatana
Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand. Electronic address: atitaya.sir@mahidol.ac.th.
Atitaya
A
Siripinyanond
D001565:Benzoates / Q000737:chemistry; D005591:Chemical Fractionation; D019343:Citric Acid / Q000737:chemistry; D031521:Fractionation, Field Flow / Q000379:methods; D053768:Metal Nanoparticles / Q000032:analysis*; D012834:Silver / Q000302:isolation & purification*; D013634:Tannins / Q000737:chemistry*
D001565:Benzoates; D013634:Tannins; D019343:Citric Acid; D012834:Silver; C006238:FL-70
Hollow fiber flow field-flow fractionation; Inductively coupled plasma mass spectrometry; Modifier; Silver nanoparticles; Tannic acid