Bacteriophage cocktail significantly reduces or eliminates Listeria monocytogenes contamination on lettuce, apples, cheese, smoked salmon and frozen foods.
ListShield™, a commercially available bacteriophage cocktail that specifically targets Listeria monocytogenes, was evaluated as a bio-control agent for L. monocytogenes in various Ready-To-Eat foods. ListShield™ treatment of experimentally contaminated lettuce, cheese, smoked salmon, and frozen entrèes significantly reduced (p < 0.05) L. monocytogenes contamination by 91% (1.1 log), 82% (0.7 log), 90% (1.0 log), and 99% (2.2 log), respectively. ListShield™ application, alone or combined with an antioxidant/anti-browning solution, resulted in a statistically significant (p < 0.001) 93% (1.1 log) reduction of L. monocytogenes contamination on apple slices after 24 h at 4 °C. Treatment of smoked salmon from a commercial processing facility with ListShield™ eliminated L. monocytogenes (no detectable L. monocytogenes) in both the naturally contaminated and experimentally contaminated salmon fillets. The organoleptic quality of foods was not affected by application of ListShield™, as no differences in the color, taste, or appearance were detectable. Bio-control of L. monocytogenes with lytic bacteriophage preparations such as ListShield™ can offer an environmentally-friendly, green approach for reducing the risk of listeriosis associated with the consumption of various foods that may be contaminated with L. monocytogenes.
Intralytix, Inc., 701 East Pratt Street, Baltimore, MD 21202, USA.
Meenu N
MN
Perera
Intralytix, Inc., 701 East Pratt Street, Baltimore, MD 21202, USA.
Tamar
T
Abuladze
Intralytix, Inc., 701 East Pratt Street, Baltimore, MD 21202, USA.
Manrong
M
Li
Intralytix, Inc., 701 East Pratt Street, Baltimore, MD 21202, USA. Electronic address: jwoolston@intralytix.com.
Joelle
J
Woolston
Intralytix, Inc., 701 East Pratt Street, Baltimore, MD 21202, USA.
Alexander
A
Sulakvelidze
D000818:Animals; D001435:Bacteriophages / Q000502:physiology*; D002611:Cheese / Q000382:microbiology*; D005396:Fish Products / Q000382:microbiology*; D005506:Food Contamination / Q000517:prevention & control*; D005519:Food Preservation / Q000379:methods*; D005628:Frozen Foods / Q000382:microbiology*; D018545:Lactuca / Q000382:microbiology*; D008089:Listeria monocytogenes / Q000254:growth & development / Q000821:virology*; D027845:Malus / Q000382:microbiology*; D012474:Salmon / Q000382:microbiology
Bacteriophage; Food safety; ListShield™; Listeria monocytogenes; Organoleptic testing; Phage bio-control