Ultraviolet B efficacy in improving antileishmanial effects of silver nanoparticles.
OBJECTIVE
Cutaneous Leishmaniasis (CL) is a parasitic disease caused by various species of the flagellated protozoan, Leishmania. Regardless of the numerous studies, there are still serious challenges in the treatment of CL. This study aimed at evaluating the influence of a low dose ultraviolet B (UVB) radiation along with silver nanoparticles (AgNPs) on a mouse model of CL induced by Leishmania major.


METHODS
L. major promastigotes (MRHO/IR/75/ER) were extracted from infected mice spleens. Two months after subcutaneous injection of 2×10(6) promastigotes into the footpad of BALB/c mice, when the lesions were developed, the animals were divided into 4 groups including one control group and three study groups: AgNPs, UVB and UVB plus AgNPs. Spleen parasite burden was assessed on day 40 after the first treatment. The data were analyzed by Instat, Elida and SPSS 16 software programs.


RESULTS
The results showed the highest pronounced inhibitory effect in the group receiving AgNPs plus UVB. In addition, a significant difference was obtained between the group receiving AgNPs alone and the one with combinational therapy. The findings on parasite burden showed a significant difference between the control group and other treatment groups.


CONCLUSIONS
It could be suggested that UVB in the presence of AgNPs, by inhibiting the spread of CL lesions and reducing the rate of visceral progression of the disease, provides a serious anti-leishmanial effect.
Department of Medical Physics, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Khadije
K
Mayelifar
Research Center for Skin Diseases and Cutaneous Leishmaniasis, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Ahmad Reza
AR
Taheri
Research Center of Medicinal Chemistry, Mashhad University of Medical Sciences, Mashhad, Iran.
Omid
O
Rajabi
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Ameneh
A
Sazgarnia
Cutaneous leishmaniasis; Leishmania major; Nanosilver; Parasite Burden; Phototherapy; Ultraviolet B