Association of GSTM1 and GSTT1 deletion polymorphisms with Pakistani aplastic anemia patients and controls and meta-analysis.
Interaction of environmental and genetic elements plays a vital role in the pathogenesis of aplastic anemia (AA). Glutathione S-transferase (GST) is a key detoxifying enzyme. Absence or low levels of this enzyme may genetically predispose individuals to AA. GST genes GSTM1 and GSTT1 are polymorphic. The aim of this study was to screen Pakistani AA patients and controls for GSTM1 deletion GSTM0 and GSTT1 deletion GSTT0 and perform meta-analysis using our data and other published data regarding these polymorphisms. DNA samples from 137 patients and 220 controls were screened using multiplex polymerase chain reaction. GSTM0 emerged as susceptible genotype for AA in Pakistan with a percentage frequency of 49.6 % as compared to 30 % in controls with odds ratio (OR) of 2.25, 95 % confidence interval (CI) of 1.4-3.5 and corrected p = 0.006. The meta-analysis showed a significant association between the null genotype GSTT0 and AA in overall analysis with OR of 1.47, 95 % CI of 1.01-2.13 and p value of 0.04 in random effects model. Studies like these could play a role in understanding the underlying path in AA pathogenesis and therefore can help in designing means for prevention, diagnose and treatment.
Institute of Biomedical and Genetic Engineering Islamabad, G9/1 Mauve Area, Islamabad, 44000, Pakistan. sadiawasiq@gmail.com.
Sadia
S
Rehman
Armed Forces Institute of Bone Marrow Transplantation Centre, Rawalpindi, Pakistan. parvez101@yahoo.com.
Parvez
P
Ahmed
Institute of Biomedical and Genetic Engineering Islamabad, G9/1 Mauve Area, Islamabad, 44000, Pakistan. nusratsaba@yahoo.co.in.
Nusrat
N
Saba
Institute of Biomedical and Genetic Engineering Islamabad, G9/1 Mauve Area, Islamabad, 44000, Pakistan. saeedaonline@yahoo.com.
Saeeda
S
Munir
Institute of Biomedical and Genetic Engineering Islamabad, G9/1 Mauve Area, Islamabad, 44000, Pakistan. simi.sajjad34@yahoo.com.
Sumaira
S
Sajjad
Armed Forces Institute of Bone Marrow Transplantation Centre, Rawalpindi, Pakistan. tariqmehmood_satti@yahoo.com.
Tariq Mehmood
TM
Satti
Armed Forces Institute of Bone Marrow Transplantation Centre, Rawalpindi, Pakistan. qammarchaudary@yahoo.com.
Qamar-un-Nisa
QU
Chaudary
Institute of Biomedical and Genetic Engineering Islamabad, G9/1 Mauve Area, Islamabad, 44000, Pakistan. atikamansoor@hotmail.com.
Atika
A
Mansoor
D000741:Anemia, Aplastic / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D005260:Female; D020022:Genetic Predisposition to Disease; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D010154:Pakistan; D011110:Polymorphism, Genetic; D017384:Sequence Deletion
D005982:Glutathione Transferase; C413545:glutathione S-transferase T1
Aplastic anemia; GST polymorphism; Pakistan