title: Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome.
abstract: The serial coupling of a reversed-phase liquid chromatography (RPLC) column to a hydrophilic interaction liquid chromatography (HILIC) column has been developed in recent years for the detection of polar and nonpolar metabolites. TCA intermediates, bile acid standards and numerous polar and non-polar metabolites extracted from beer were analysed using a combined RPLC/HILIC method. Non-polar metabolites were retained by the RPLC column. Polar metabolites not retained by the RPLC column were retained and separated by the HILIC column. The results from this study validate this simple yet powerful metabolomics approach.
authors.affiliation: Polyomics, University of Glasgow, 211 Wolfson Wohl Translational Cancer Research Centre, Garscube Campus, Glasgow, G61 1QH UK.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Haggarty
authors.affiliation: Thermo Fisher Scientific, Hemel Hempstead, UK.
authors.forename: Madalina
authors.initials: M
authors.lastname: Oppermann
authors.affiliation: Centre for Cell Engineering, University of Glasgow, Glasgow, UK.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Dalby
authors.affiliation: Polyomics, University of Glasgow, 211 Wolfson Wohl Translational Cancer Research Centre, Garscube Campus, Glasgow, G61 1QH UK.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Burchmore
authors.affiliation: Thermo Fisher Scientific, Hemel Hempstead, UK.
authors.forename: Ken
authors.initials: K
authors.lastname: Cook
authors.affiliation: Polyomics, University of Glasgow, 211 Wolfson Wohl Translational Cancer Research Centre, Garscube Campus, Glasgow, G61 1QH UK.
authors.forename: Stefan
authors.initials: S
authors.lastname: Weidt
authors.affiliation: Polyomics, University of Glasgow, 211 Wolfson Wohl Translational Cancer Research Centre, Garscube Campus, Glasgow, G61 1QH UK.
authors.forename: Karl E V
authors.initials: KE
authors.lastname: Burgess
keywords: Beer; Bile acids; HILIC; LC–MS; Organic acids; Reversed phase