title: Axial range of conjugate adaptive optics in two-photon microscopy.
abstract: We describe an adaptive optics technique for two-photon microscopy in which the deformable mirror used for aberration compensation is positioned in a plane conjugate to the plane of the aberration. We demonstrate in a proof-of-principle experiment that this technique yields a large field of view advantage in comparison to standard pupil-conjugate adaptive optics. Further, we show that the extended field of view in conjugate AO is maintained over a relatively large axial translation of the deformable mirror with respect to the conjugate plane. We conclude with a discussion of limitations and prospects for the conjugate AO technique in two-photon biological microscopy.
authors.forename: Hari P
authors.initials: HP
authors.lastname: Paudel
authors.forename: John
authors.initials: J
authors.lastname: Taranto
authors.forename: Jerome
authors.initials: J
authors.lastname: Mertz
authors.forename: Thomas
authors.initials: T
authors.lastname: Bifano