title: Structural and biochemical characterization and evolutionary relationships of the fatty acid-binding protein 10 (Fabp10) of hake (Merluccius hubbsi).
abstract: A fatty acid-binding protein (FABP) from the liver of Argentine hake (Merluccius hubbsi) was isolated and characterized and its expression analyzed. The determination of its partial primary structures (72%) showed that it presents highest identity with Fabp10, commonly termed liver basic-type FABP. The evolutionary tree showed greater relationship between the Fabp10 of hake (Me Fabp10) and the Fabp10 and the Fabp10a of teleost fish. Me Fabp10 had low affinity for palmitic, oleic and palmitoleic acid and high affinity for bilirubin, lysophosphatidylcholine and lysophosphatidylethanolamine, all of them important in the metabolic functions of the liver. Me Fabp10 was able to bind only one cis-parinaric acid molecule and was found to be expressed only in the liver.
authors.affiliation: Departamento de Bioquímica, GQBMRNP-CRIDECIT, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia San Juan Bosco, 9000, Comodoro Rivadavia, Chubut, Argentina.
authors.forename: Cecilia Alejandra
authors.initials: CA
authors.lastname: Crovetto
authors.affiliation: Departamento de Bioquímica, GQBMRNP-CRIDECIT, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia San Juan Bosco, 9000, Comodoro Rivadavia, Chubut, Argentina. okylola@gmail.com.
authors.forename: Osvaldo León
authors.initials: OL
authors.lastname: Córdoba
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019143:Evolution, Molecular; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D048388:Gadiformes / Q000235:genetics / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D050556:Fatty Acid-Binding Proteins; D029941:Fish Proteins
keywords: Fabp10; Fatty acid-binding protein; Hake; Liver