title: E. adenophorum Induces Cell Cycle and Apoptosis of Renal Cells through Mitochondrial Pathway and Caspase Activation in Saanen Goat.
abstract: The cytotoxicity effects of E. adenophorum on cell cycle and apoptosis of renal cells in Saanen goat was evaluated by TUNEL, DAPI, AO/EB staining, DNA fragmentation assay, Caspase activity, Western-blot, qRT-PCR and flow cytometry analysis. 16 saanen goats randomly divided into four groups were fed on 0%, 40%, 60% and 80% E. adenophorum diets. The Results showed that E. adenophorum induced typical apoptotic features of renal cells. E. adenophorum significantly suppressed renal cells viability, caused cell cycle activity arrest and induced typical apoptotic features in a dose-dependent manner. However, the protein levels of Fas/FasL, Bid and caspase-8 did not appear significant changes in the process of E. adenophorum-induced apoptosis. Moreover, E. adenophorum administration slightly decreased Bcl-2 expression, promoted Bax translocation to mitochondria, triggered the release of Cyt c from mitochondria into cytosol and activated caspase-9, -3, and cleaved PARP. The mitochondrial p53 translocation was significantly activated, accompanied by a significant increase in the loss of ΔΨm, Cyt c release and caspase-9 activation. Above all, these data suggest that E. adenophorum induces renal cells apoptosis via the activation of mitochondria-mediated apoptosis pathway in renal cells. These findings may provide new insights to understand the mechanisms involved in E. adenophorum-caused cytotoxicity of renal cells.
authors.affiliation: Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan Province, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: He
authors.affiliation: Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan Province, China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Chen
authors.affiliation: Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan Province, China.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan Province, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Luo
authors.affiliation: Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan Province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.affiliation: Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan Province, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Mo
authors.affiliation: Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan Province, China.
authors.forename: Ruiguang
authors.initials: R
authors.lastname: Xu
authors.affiliation: Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan Province, China.
authors.forename: Yancheng
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan Province, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Ren
authors.affiliation: Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan Province, China.
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.affiliation: Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan Province, China.
authors.forename: Junliang
authors.initials: J
authors.lastname: Deng
authors.affiliation: Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan Province, China.
authors.forename: Guangneng
authors.initials: G
authors.lastname: Peng
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, School of Life Science, Northwest University, Xi'an, China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, School of Life Science, Northwest University, Xi'an, China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Wei
mesh_terms: D036603:Ageratina; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D020169:Caspases / Q000378:metabolism*; D002453:Cell Cycle / Q000502:physiology*; D059447:Cell Cycle Checkpoints / Q000502:physiology; D006041:Goats; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D020169:Caspases