title: Skin mucus proteome map of European sea bass (Dicentrarchus labrax).
abstract: Skin mucus is the first barrier of fish defence. Proteins from skin mucus of European sea bass (Dicentrarchus labrax) were identified by 2DE followed by LC-MS/MS. From all the identified proteins in the proteome map, we focus on the proteins associated with several immune pathways in fish. Furthermore, the real-time PCR transcript levels in skin are shown. Proteins found include apolipoprotein A1, calmodulin, complement C3, fucose-binding lectin, lysozyme and several caspases. To our knowledge, this is the first skin mucus proteome study and further transcriptional profiling of the identified proteins done on this bony fish species. This not only contributes knowledge on the routes involved in mucosal innate immunity, but also establishes a non-invasive technique based on locating immune markers with a potential use for prevention and/or diagnosis of fish diseases.
authors.affiliation: Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, Murcia, Spain.|Faculty of Biosciences and Aquaculture, University of Nordland, Bodø, Norway.
authors.forename: Héctor
authors.initials: H
authors.lastname: Cordero
authors.affiliation: Faculty of Biosciences and Aquaculture, University of Nordland, Bodø, Norway.
authors.forename: Monica F
authors.initials: MF
authors.lastname: Brinchmann
authors.affiliation: Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, Murcia, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Cuesta
authors.affiliation: Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, Murcia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Meseguer
authors.affiliation: Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, Murcia, Spain.
authors.forename: María A
authors.initials: MA
authors.lastname: Esteban
mesh_terms: D000818:Animals; D001492:Bass / Q000378:metabolism*; D009093:Mucus / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome
keywords: European sea bass (Dicentrarchus labrax); Mucosal immunity; Proteome; Skin mucus; Transcript profile