title: Engineering cell-compatible paper chips for cell culturing, drug screening, and mass spectrometric sensing.
abstract: Paper-supported cell culture is an unprecedented development for advanced bioassays. This study reports a strategy for in vitro engineering of cell-compatible paper chips that allow for adherent cell culture, quantitative assessment of drug efficiency, and label-free sensing of intracellular molecules via paper spray mass spectrometry. The polycarbonate paper is employed as an excellent alternative bioscaffold for cell distribution, adhesion, and growth, as well as allowing for fluorescence imaging without light scattering. The cell-cultured paper chips are thus amenable to fabricate 3D tissue construction and cocultures by flexible deformation, stacks and assembly by layers of cells. As a result, the successful development of cell-compatible paper chips subsequently offers a uniquely flexible approach for in situ sensing of live cell components by paper spray mass spectrometry, allowing profiling the cellular lipids and quantitative measurement of drug metabolism with minimum sample pretreatment. Consequently, the developed paper chips for adherent cell culture are inexpensive for one-time use, compatible with high throughputs, and amenable to label-free and rapid analysis.
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing, 100084, P.R. China.
authors.forename: Qiushui
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing, 100084, P.R. China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing, 100084, P.R. China.
authors.forename: Wu
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing, 100084, P.R. China.
authors.forename: Xuexia
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing, 100084, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing, 100084, P.R. China.
authors.forename: Haifang
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing, 100084, P.R. China.
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Lin
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001681:Biological Assay; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D060846:Cell Engineering / Q000379:methods*; D004353:Drug Evaluation, Preclinical; D013058:Mass Spectrometry; D051379:Mice; D041681:NIH 3T3 Cells; D010209:Paper; D011076:Polycarboxylate Cement / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011076:Polycarboxylate Cement; C005506:polycarbonate
keywords: 3D culture; drug assays; mass spectrometric sensing; paper chips; paper-supported cell culture