title: Nanoparticles Containing Curcumin Useful for Suppressing Macrophages In Vivo in Mice.
abstract: To explore a novel method using liposomes to suppress macrophages, we screened food constituents through cell culture assays. Curcumin was one of the strongest compounds exhibiting suppressive effects on macrophages. We subsequently tried various methods to prepare liposomal curcumin, and eventually succeeded in preparing liposomes with sufficient amounts of curcumin to suppress macrophages by incorporating a complex of curcumin and bovine serum albumin. The diameter of the resultant nanoparticles, the liposomes containing curcumin, ranged from 60 to 100 nm. Flow cytometric analyses revealed that after intraperitoneal administration of the liposomes containing curcumin into mice, these were incorporated mainly by macrophages positive for F4/80, CD36, and CD11b antigens. Peritoneal cells prepared from mice injected in vivo with the liposomes containing curcumin apparently decreased interleukin-6-producing activities. Major changes in body weight and survival rates in the mice were not observed after administrating the liposomes containing curcumin. These results indicate that the liposomes containing curcumin are safe and useful for the selective suppression of macrophages in vivo in mice.
authors.affiliation: R&D Division, Katayama Chemical Industries, Co., Ltd., Minoh-City, Osaka, Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Amano
authors.affiliation: R&D Division, Katayama Chemical Industries, Co., Ltd., Minoh-City, Osaka, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Minematsu
authors.affiliation: Department of Food and Nutrition, Faculty of Human Life Science, Senri Kinran University, Suita-City, Osaka, Japan.
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Fujita
authors.affiliation: R&D Division, Katayama Chemical Industries, Co., Ltd., Minoh-City, Osaka, Japan.
authors.forename: Shinki
authors.initials: S
authors.lastname: Iwashita
authors.affiliation: R&D Division, Katayama Chemical Industries, Co., Ltd., Minoh-City, Osaka, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Adachi
authors.affiliation: R&D Division, Katayama Chemical Industries, Co., Ltd., Minoh-City, Osaka, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Igarashi
authors.affiliation: Department of Food and Nutrition, Faculty of Human Life Science, Senri Kinran University, Suita-City, Osaka, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Hinuma
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000008:administration & dosage* / Q000009:adverse effects; D005260:Female; D005504:Food Analysis; D006801:Humans; D016130:Immunophenotyping; D015850:Interleukin-6 / Q000096:biosynthesis; D008081:Liposomes; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D053758:Nanoparticles
chemical_list: D000954:Antigens, Surface; D015850:Interleukin-6; D008081:Liposomes; D003474:Curcumin