title: MiR-634 decreases cell proliferation and induces apoptosis by targeting mTOR signaling pathway in cervical cancer cells.
abstract: Taking the emergence of continuous resistance to chemotherapy and the evidence that miRNAs are associated with chemoresistance in cancers into consideration, it is of significant importance to reveal the miRNAs functions for the treatment of cancer. As a novel tumor suppressor, MiR-634 is known to induce apoptosis in tumor cell which is essential for tumorigenesis. Herein, we elucidated the regulation effects of miR-634 in gene expression and discovery of its target gene in cell proliferation and invasion that would aid therapeutic apoptosis. As a result, by targeting mTOR signal pathway, miR-634 inhibited cell proliferation, migration and invasiveness in cervical cancer cells and the block of miR-634 enhances the mTOR expression at both the mRNA and protein levels which regulated the expression of mTOR negatively. Taken together, these results further indicated that miR-634 is an effective target for cancer treatment, and the findings provided in this work might lead to the better understanding of the malignant behavior of cervical carcinoma.
authors.affiliation: a Department of Gynaecology , Qilu Hospital, Shandong Univeristy , China .|b Department of Gynaecology , Qingdao University affiliated Yantai Yuhuangding Hospital , Shandong , China , and.
authors.forename: Jianglin
authors.initials: J
authors.lastname: Cong
authors.affiliation: c Department of Laboratory , Qingdao University affiliated Yantai Yuhuangding Hospital , Shandong , China.
authors.forename: Riming
authors.initials: R
authors.lastname: Liu
authors.affiliation: b Department of Gynaecology , Qingdao University affiliated Yantai Yuhuangding Hospital , Shandong , China , and.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: b Department of Gynaecology , Qingdao University affiliated Yantai Yuhuangding Hospital , Shandong , China , and.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Jiang
authors.affiliation: a Department of Gynaecology , Qilu Hospital, Shandong Univeristy , China .
authors.forename: Youzhong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000328:Adult; D017209:Apoptosis; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000096:biosynthesis*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: C000599270:MIRN-634 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases
keywords: cell proliferation; cervical cancer; mTOR expression; microRNA