title: Increase of serum interleukin 6 and interferon γ is associated with the number of impulses in patients with supraventricular arrhythmias treated with radiofrequency catheter ablation.
abstract: BACKGROUND
  Activation of the immune system plays a pathogenic role in the process of myocardial remodeling in patients with supraventricular arrhythmias. The intensity of this process is associated with the effectiveness of electrical cardioversion and radiofrequency catheter ablation (RFA). The aim of this study was to test the ability of the biochip microarray to detect immune parameters in patients with supraventricular arrhythmias undergoing RFA treatment.
  
  
  METHODS
  We used a biochip-based microarray system to determine multiple immune parameters in a group of 35 patients who had undergone RFA for atrioventricular nodal reentry tachycardia (AVNRT), atrial flutter (AFL) and atrial fibrillation (AF).
  
  
  RESULTS
  Before the procedure, serum IL-6 and VEGF levels were significantly increased in patients with atrial fibrillation compared to patients with AVNRT (IL-6: 6.4±6.3 ng/L vs. 1.5±0.7 ng/L, P < 0.01; VEGF: 132.4±74 ng/L vs. 88.5±56.4 ng/L, P < 0.01). After the procedure, serum IL-6, VEGF, IFN-γ and MCP-1 levels significantly increased compared to baseline (IL-6: 5.2±4.8 ng/L vs. 2.9±2.1 ng/L, P < 0.01; VEGF: 195.8±160 ng/L vs. 119.8± 110 ng/L, P < 0.05; IFN-γ: 3.1±1.2 ng/L vs. 2.3±0.6 ng/L, P < 0.05; MCP-1: 104.1±84.5 ng/L vs. 54.5±50 ng/L, P < 0.05). Serum IL-6 and IFN-γ were associated with the number of RFA applications (IL-6: r = 0.56, n 33; IFN-γ: r = 0.47, n 33).
  
  
  CONCLUSIONS
  This study showed that biochip-based microarray can be useful in the detection of immune activation in patients with arrhythmias and can detect myocardial injury after RF procedures.
authors.affiliation: 1st Deptartment of Internal Medicine - Cardioangiology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic.
authors.forename: Radek
authors.initials: R
authors.lastname: Pudil
authors.affiliation: Institute of Clinical Biochemistry and Diagnostics, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Vasatova
authors.affiliation: 1st Deptartment of Internal Medicine - Cardioangiology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Parizek
authors.affiliation: 1st Deptartment of Internal Medicine - Cardioangiology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic.
authors.forename: Ludek
authors.initials: L
authors.lastname: Haman
authors.affiliation: 1st Deptartment of Internal Medicine - Cardioangiology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Horakova
authors.affiliation: Institute of Clinical Biochemistry and Diagnostics, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Palicka
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D017115:Catheter Ablation / Q000379:methods*; D004815:Epidermal Growth Factor / Q000378:metabolism; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D008297:Male; D046228:Microarray Analysis / Q000379:methods; D008875:Middle Aged; D013611:Tachycardia, Atrioventricular Nodal Reentry / Q000097:blood / Q000276:immunology / Q000601:surgery*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D055815:Young Adult
chemical_list: D015415:Biomarkers; D015850:Interleukin-6; D042461:Vascular Endothelial Growth Factor A; D004815:Epidermal Growth Factor; D007371:Interferon-gamma
keywords: cardiology; cytokines; myocardial injury; radiofrequency catheter ablation; supraventricular arrhythmia