title: Transcriptomic response of Saccharomyces cerevisiae for its adaptation to sulphuric acid-induced stress.
abstract: In bioethanol production plants, yeast cells are generally recycled between fermentation batches by using a treatment with sulphuric acid at a pH ranging from 2.0 to 2.5. We have previously shown that Saccharomyces cerevisiae cells exposed to sulphuric acid treatment induce the general stress response pathway, fail to activate the protein kinase A signalling cascade and requires the mechanisms of cell wall integrity and high osmolarity glycerol pathways in order to survive in this stressful condition. In the present work, we used transcriptome-wide analysis as well as physiological assays to identify the transient metabolic responses of S. cerevisiae under sulphuric acid treatment. The results presented herein indicate that survival depends on a metabolic reprogramming of the yeast cells in order to assure the yeast cell viability by preventing cell growth under this harmful condition. It involves the differential expression of a subset of genes related to cell wall composition and integrity, oxidation-reduction processes, carbohydrate metabolism, ATP synthesis and iron uptake. These results open prospects for application of this knowledge in the improvement of industrial processes based on metabolic engineering to select yeasts resistant to acid treatment.
authors.affiliation: Interdepartmental Research Group in Metabolic Engineering, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Rodrigo Mendonça
authors.initials: RM
authors.lastname: de Lucena
authors.affiliation: Interdepartmental Research Group in Metabolic Engineering, Federal University of Pernambuco, Recife, Brazil.|Department of Biochemistry, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Elsztein
authors.affiliation: Interdepartmental Research Group in Metabolic Engineering, Federal University of Pernambuco, Recife, Brazil.|Department of Antibiotics, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Will
authors.initials: W
authors.lastname: de Barros Pita
authors.affiliation: Interdepartmental Research Group in Metabolic Engineering, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Rafael Barros
authors.initials: RB
authors.lastname: de Souza
authors.affiliation: Laboratory of Bioinformatics and Evolutionary Biology, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Sérgio
authors.initials: S
authors.lastname: de Sá Leitão Paiva Júnior
authors.affiliation: Interdepartmental Research Group in Metabolic Engineering, Federal University of Pernambuco, Recife, Brazil. marcos.moraisjr@ufpe.br.|Department of Genetics, Federal University of Pernambuco, Av. Moraes Rego, 1235 Cidade Universitária, Recife, PE, 50670-901, Brazil. marcos.moraisjr@ufpe.br.
authors.forename: Marcos Antonio
authors.initials: MA
authors.lastname: de Morais Junior
mesh_terms: D000220:Adaptation, Biological; D050260:Carbohydrate Metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D009154:Mutation; D018384:Oxidative Stress; D011687:Purines / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000502:physiology*; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics*; D013464:Sulfuric Acids / Q000494:pharmacology*; D059467:Transcriptome
chemical_list: D011687:Purines; D013464:Sulfuric Acids; D000431:Ethanol; D007501:Iron; C033158:sulfuric acid; C030985:purine
keywords: Cell wall integrity; Ethanol fermentation; Metabolic signalling; Stress response