title: Tumour necrosis factor-alpha-induced protein 8 (TNFAIP8) expression associated with cell survival and death in cancer cell lines infected with canine distemper virus.
abstract: Oncolytic virotherapy is a novel strategy for treatment of cancer in humans and companion animals as well. Canine distemper virus (CDV), a paramyxovirus, has proven to be oncolytic through induction of apoptosis in canine-derived tumour cells, yet the mechanism behind this inhibitory action is poorly understood. In this study, three human mammary tumour cell lines and one canine-derived adenofibrosarcoma cell line were tested regarding to their susceptibility to CDV infection, cell proliferation, apoptosis, mitochondrial membrane potential and expression of tumour necrosis factor-alpha-induced protein 8 (TNFAIP8). CDV replication-induced cytopathic effect, decrease of cell proliferation rates, and >45% of infected cells were considered death and/or under late apoptosis/necrosis. TNFAIP8 and CDVM gene expression were positively correlated in all cell lines. In addition, mitochondrial membrane depolarization was associated with increase in virus titres (p < 0.005). Thus, these results strongly suggest that both human and canine mammary tumour cells are potential candidates for studies concerning CDV-induced cancer therapy.
authors.affiliation: Veterinary Medicine School, Department of Support, Production and Animal Health, University of São Paulo State, Laboratory of Animal Virology and Cell Culture, Araçatuba, São Paulo, Brazil.
authors.forename: J A
authors.initials: JA
authors.lastname: Garcia
authors.affiliation: FZEA-USP, Department of de Veterinary Medicine, Pirassununga, São Paulo, Brazil.
authors.forename: H L
authors.initials: HL
authors.lastname: Ferreira
authors.affiliation: Veterinary Medicine School, Department of Support, Production and Animal Health, University of São Paulo State, Laboratory of Animal Virology and Cell Culture, Araçatuba, São Paulo, Brazil.|Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, São Paulo, Brazil.
authors.forename: F V
authors.initials: FV
authors.lastname: Vieira
authors.affiliation: Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, São Paulo, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Gameiro
authors.affiliation: Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, São Paulo, Brazil.
authors.forename: A L
authors.initials: AL
authors.lastname: Andrade
authors.affiliation: Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, São Paulo, Brazil.
authors.forename: F R
authors.initials: FR
authors.lastname: Eugênio
authors.affiliation: Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: E F
authors.initials: EF
authors.lastname: Flores
authors.affiliation: Veterinary Medicine School, Department of Support, Production and Animal Health, University of São Paulo State, Laboratory of Animal Virology and Cell Culture, Araçatuba, São Paulo, Brazil.|Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, São Paulo, Brazil.
authors.forename: T C
authors.initials: TC
authors.lastname: Cardoso
mesh_terms: D000230:Adenocarcinoma / Q000628:therapy / Q000662:veterinary; D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D001943:Breast Neoplasms / Q000628:therapy; D016923:Cell Death; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004217:Distemper Virus, Canine / Q000378:metabolism*; D004283:Dog Diseases / Q000628:therapy; D004285:Dogs; D005260:Female; D006801:Humans; D015674:Mammary Neoplasms, Animal / Q000628:therapy; D050130:Oncolytic Virotherapy / Q000379:methods / Q000662:veterinary*
chemical_list: D051017:Apoptosis Regulatory Proteins; C404190:TNFAIP8 protein, human
keywords: cancer markers; canine and human mammary tumours; cell phenotype; in vitro culture