title: Development of a Rapid LC-MS/MS Method for the Determination of Emerging Fusarium mycotoxins Enniatins and Beauvericin in Human Biological Fluids.
abstract: A novel method for the simultaneous determination of enniatins A, A1, B and B1 and beauvericin, both in human urine and plasma samples, was developed and validated. The method consisted of a simple and easy pretreatment, specific for each matrix, followed by solid phase extraction (SPE) and detection by high performance liquid chromatography-tandem mass spectrometry with an electrospray ion source. The optimized SPE method was performed on graphitized carbon black cartridges after suitable dilution of the extracts, which allowed high mycotoxin absolute recoveries (76%-103%) and the removal of the major interferences from the matrix. The method was extensively evaluated for plasma and urine samples separately, providing satisfactory results in terms of linearity (R² of 0.991-0.999), process efficiency (>81%), trueness (recoveries between 85% and 120%), intra-day precision (relative standard deviation, RSD < 18%), inter-day precision (RSD < 21%) and method quantification limits (ranging between 20 ng·L(-1) and 40 ng·L(-1) in plasma and between 5 ng·L(-1) and 20 ng·L(-1) in urine). Finally, the highly sensitive validated method was applied to some urine and plasma samples from different donors.
authors.affiliation: Laboratorio de Toxicología, Departament de Medicina Preventiva I Salut Pública, Ciències de l'Alimentació, Toxicologia I Medicina Legal Facultat de Farmàcia, Universitat de València, València 46010, Spain. A.Belen.Serrano@uv.es.
authors.forename: Ana
authors.initials: A
authors.lastname: Belén Serrano
authors.affiliation: Department of Chemistry, Università di Roma "La Sapienza", Piazzale Aldo Moro 5, Rome 00185, Italy. annalaura.capriotti@uniroma1.it.
authors.forename: Anna Laura
authors.initials: AL
authors.lastname: Capriotti
authors.affiliation: Department of Chemistry, Università di Roma "La Sapienza", Piazzale Aldo Moro 5, Rome 00185, Italy. chiara.cavaliere@uniroma1.it.
authors.forename: Chiara
authors.initials: C
authors.lastname: Cavaliere
authors.affiliation: Department of Chemistry, Università di Roma "La Sapienza", Piazzale Aldo Moro 5, Rome 00185, Italy. susy.piovesana@uniroma1.it.
authors.forename: Susy
authors.initials: S
authors.lastname: Piovesana
authors.affiliation: Department of Chemistry, Università di Roma "La Sapienza", Piazzale Aldo Moro 5, Rome 00185, Italy. roberto.samperi@uniroma1.it.
authors.forename: Roberto
authors.initials: R
authors.lastname: Samperi
authors.affiliation: Department of Chemistry, Università di Roma "La Sapienza", Piazzale Aldo Moro 5, Rome 00185, Italy. salvatore.ventura@uniroma1.it.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Ventura
authors.affiliation: Department of Chemistry, Università di Roma "La Sapienza", Piazzale Aldo Moro 5, Rome 00185, Italy. aldo.lagana@uniroma1.it.
authors.forename: Aldo
authors.initials: A
authors.lastname: Laganà
mesh_terms: D000328:Adult; D000368:Aged; D002853:Chromatography, Liquid; D047630:Depsipeptides / Q000097:blood* / Q000652:urine*; D005260:Female; D005670:Fusarium / Q000378:metabolism; D006801:Humans; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D047630:Depsipeptides; C100264:enniatins; C004456:beauvericin
keywords: beauvericin; enniatins; liquid chromatography-tandem mass spectrometry; mycotoxins; plasma; urine