title: Low-concentration uranium enters the HepG2 cell nucleus rapidly and induces cell stress response.
abstract: This study aimed to compare the cell stress effects of low and high uranium concentrations and relate them to its localization, precipitate formation, and exposure time. The time-course analysis shows that uranium appears in cell nuclei as a soluble form within 5 min of exposure, and quickly induces expression of antioxidant and DNA repair genes. On the other hand, precipitate formations began at the very beginning of exposure at the 300-μM concentration, but took longer to appear at lower concentrations. Adaptive response might occur at low concentrations but are overwhelmed at high concentrations, especially when uranium precipitates are abundant.
authors.affiliation: Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-HOM, SRBE, LRTOX, Fontenay-aux-Roses, France. Electronic address: yann.gueguen@irsn.fr.
authors.forename: Yann
authors.initials: Y
authors.lastname: Guéguen
authors.affiliation: Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-HOM, SDI, LRC, Fontenay-aux-Roses, France.
authors.forename: David
authors.initials: D
authors.lastname: Suhard
authors.affiliation: Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-HOM, SRBE, LRTOX, Fontenay-aux-Roses, France.
authors.forename: Clémentine
authors.initials: C
authors.lastname: Poisson
authors.affiliation: Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-HOM, SRBE, LRTOX, Fontenay-aux-Roses, France.
authors.forename: Line
authors.initials: L
authors.lastname: Manens
authors.affiliation: Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-HOM, SRBE, LRTOX, Fontenay-aux-Roses, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Elie
authors.affiliation: Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-HOM, SDI, LRC, Fontenay-aux-Roses, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Landon
authors.affiliation: Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-HOM, SDI, LRC, Fontenay-aux-Roses, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Bouvier-Capely
authors.affiliation: Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-HOM, SRBE, LRTOX, Fontenay-aux-Roses, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Rouas
authors.affiliation: Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-HOM, SRBE, Fontenay-aux-Roses, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Benderitter
authors.affiliation: Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-HOM, SDI, LRC, Fontenay-aux-Roses, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Tessier
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D002467:Cell Nucleus / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D056945:Hep G2 Cells; D006801:Humans; D018384:Oxidative Stress / Q000528:radiation effects; D013312:Stress, Physiological / Q000528:radiation effects*; D014501:Uranium / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D014501:Uranium
keywords: HepG2; Localization; Low-dose; RedOx; Uranium