title: DNA Polymer Brush Patterning through Photocontrollable Surface-Initiated DNA Hybridization Chain Reaction.
abstract: The fabrication of DNA polymer brushes with spatial resolution onto a solid surface is a crucial step for biochip research and related applications, cell-free gene expression study, and even artificial cell fabrication. Here, for the first time, a DNA polymer brush patterning method is reported based on the photoactivation of an ortho-nitrobenzyl linker-embedded DNA hairpin structure and a subsequent surface-initiated DNA hybridization chain reaction (HCR). Inert DNA hairpins are exposed to ultraviolet light irradiation to generate DNA duplexes with two active sticky ends (toeholds) in a programmable manner. These activated DNA duplexes can initiate DNA HCR to generate multifunctional patterned DNA polymer brushes with complex geometrical shapes. Different multifunctional DNA polymer brush patterns can be fabricated on certain areas of the same solid surface using this method. Moreover, the patterned DNA brush surface can be used to capture target molecules in a desired manner.
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China.
authors.forename: Fujian
authors.initials: F
authors.lastname: Huang
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China.
authors.forename: Dongbao
authors.initials: D
authors.lastname: Yao
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China.
authors.forename: Shiyan
authors.initials: S
authors.lastname: Xiao
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China.|Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China.
authors.forename: Haojun
authors.initials: H
authors.lastname: Liang
mesh_terms: D000327:Adsorption; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects; D015342:DNA Probes / Q000138:chemical synthesis* / Q000235:genetics / Q000528:radiation effects; D017403:In Situ Hybridization / Q000379:methods*; D008027:Light; D008422:Materials Testing; D054802:Molecular Imprinting / Q000379:methods*; D010777:Photochemistry / Q000379:methods; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties / Q000528:radiation effects
chemical_list: D015342:DNA Probes; D011108:Polymers; D004247:DNA
keywords: DNA polymer brushes; photocontrollable reactions; surface patterning; surface-initiated DNA hybridization chain reactions; target molecule capture