title: Different metabolic activity in placental and reflected regions of the human amniotic membrane.
abstract: Cells of the human amniotic membrane (hAM) have stem cell characteristics with low immunogenicity and anti-inflammatory properties. While hAM is an excellent source for tissue engineering, so far, its sub-regions have not been taken into account. We show that placental and reflected hAM differ distinctly in morphology and functional activity, as the placental region has significantly higher mitochondrial activity, however significantly less reactive oxygen species. Since mitochondria may participate in processes such as cell rescue, we speculate that amniotic sub-regions may have different potential for tissue regeneration, which may be crucial for clinical applications.
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Donaueschingenstraße 13, 1200 Vienna, Austria; Austrian Cluster for Tissue Regeneration, Austria. Electronic address: asmita.banerjee@trauma.lbg.ac.at.
authors.forename: Asmita
authors.initials: A
authors.lastname: Banerjee
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Donaueschingenstraße 13, 1200 Vienna, Austria; Austrian Cluster for Tissue Regeneration, Austria.
authors.forename: Adelheid
authors.initials: A
authors.lastname: Weidinger
authors.affiliation: Genomics Core Facility, VetCore, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria.
authors.forename: Martin
authors.initials: M
authors.lastname: Hofer
authors.affiliation: Genomics Core Facility, VetCore, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria.
authors.forename: Ralf
authors.initials: R
authors.lastname: Steinborn
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Donaueschingenstraße 13, 1200 Vienna, Austria; Austrian Cluster for Tissue Regeneration, Austria.
authors.forename: Andrea
authors.initials: A
authors.lastname: Lindenmair
authors.affiliation: Red Cross Blood Transfusion Service of Upper Austria, Krankenhausstr. 7, 4017 Linz, Austria; Austrian Cluster for Tissue Regeneration, Austria.
authors.forename: Simone
authors.initials: S
authors.lastname: Hennerbichler-Lugscheider
authors.affiliation: Bio-Products & Bio-Engineering AG, Schottenring 10, 1010 Vienna, Austria.
authors.forename: Johann
authors.initials: J
authors.lastname: Eibl
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Donaueschingenstraße 13, 1200 Vienna, Austria; Austrian Cluster for Tissue Regeneration, Austria.
authors.forename: Heinz
authors.initials: H
authors.lastname: Redl
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Donaueschingenstraße 13, 1200 Vienna, Austria; Austrian Cluster for Tissue Regeneration, Austria.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Kozlov
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Donaueschingenstraße 13, 1200 Vienna, Austria; Austrian Cluster for Tissue Regeneration, Austria.
authors.forename: Susanne
authors.initials: S
authors.lastname: Wolbank
mesh_terms: D000650:Amnion / Q000166:cytology / Q000378:metabolism*; D019069:Cell Respiration; D005260:Female; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D011247:Pregnancy
keywords: Human amniotic membrane; Mitochondrial respiration; Stem cell; Tissue regeneration