title: Cytokine profile of autologous platelet-derived eye drops in patients with ocular chronic graft-versus-host disease.
abstract: Ocular chronic GVHD is efficaciously treated with autologous platelet-derived eye drops. We investigated the cytokine content of eye drops produced using a non-gelified lysate obtained from autologous platelet-rich plasma in six patients with ocular GVHD. In both the responding (n = 4) and the resistant (n = 2) patients, the eye drops were significantly enriched with various growth factors, in amounts proportional with the platelet counts. In contrast, chemokine ligand and interleukin levels were similar to those of plasma. The non-responding patients showed the highest levels of chemokine (C-X-C motif) ligand (CXCL)10. These findings provide possible explanations for beneficial or detrimental effects of eye drops.
authors.affiliation: Medicine Transfusion Department, Institute of Hematology, Catholic University, Rome, Italy.
authors.forename: C G
authors.initials: CG
authors.lastname: Valentini
authors.affiliation: Medicine Transfusion Department, Institute of Hematology, Catholic University, Rome, Italy.
authors.forename: E R
authors.initials: ER
authors.lastname: Nuzzolo
authors.affiliation: Medicine Transfusion Department, Institute of Hematology, Catholic University, Rome, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Orlando
authors.affiliation: Hematology Department, Institute of Hematology, Catholic University, Rome, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Metafuni
authors.affiliation: Medicine Transfusion Department, Institute of Hematology, Catholic University, Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Bianchi
authors.affiliation: Hematology Department, Institute of Hematology, Catholic University, Rome, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Chiusolo
authors.affiliation: Medicine Transfusion Department, Institute of Hematology, Catholic University, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Zini
authors.affiliation: Medicine Transfusion Department, Institute of Hematology, Catholic University, Rome, Italy.
authors.forename: L
authors.identifier: http://orcid.org/0000-0002-7214-1561
authors.initials: L
authors.lastname: Teofili
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000737:chemistry / Q000378:metabolism*; D016207:Cytokines / Q000032:analysis*; D005260:Female; D006086:Graft vs Host Disease / Q000188:drug therapy*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009883:Ophthalmic Solutions / Q000737:chemistry* / Q000627:therapeutic use
chemical_list: D016207:Cytokines; D009883:Ophthalmic Solutions
keywords: GVHD; autologous platelet lysates; chemokines; dry eye syndrome; growth factors