title: Impact of aflatoxin B1 on the pharmacokinetic disposition of enrofloxacin in broiler chickens.
abstract: The potential impact of subchronic exposure of aflatoxin B1 was investigated on the pharmacokinetic disposition of enrofloxacin in broiler chickens. Broiler chickens given either normal or aflatoxin B1 (750μg/kg diet) supplemented diet for 6 weeks received a single oral dose of enrofloxacin (10mg/kg body wt). Blood samples were drawn from the brachial vein at predetermined time intervals after drug administration. Enrofloxacin plasma concentrations analyzed by RP-HPLC were significantly lower in aflatoxin B1-exposed broiler chickens at 0.167, 0.5 and 1.0h after drug administration. In aflatoxin B1-exposed broiler chickens, the absorption rate constant (ka) of enrofloxacin (0.20±0.05h(-1)) was significantly decreased as compared to the unexposed birds (0.98±0.31h(-1)). The values of [Formula: see text] , tmax and AUC0-∞ of enrofloxacin were nonsignificantly increased by 17%, 26% and 17% in aflatoxin-exposed broiler chickens, respectively. Subchronic aflatoxin B1 exposure markedly decreased the initial absorption of enrofloxacin without significantly influencing other pharmacokinetic parameters in broiler chickens.
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar 243 122, UP, India. Electronic address: kalpanaivri@gmail.com.
authors.forename: Starling
authors.initials: S
authors.lastname: Kalpana
authors.affiliation: Department of Pharmacology and Toxicology, NTR College of Veterinary and Animal Sciences, Gannavaram 521102, AP, India.
authors.forename: G
authors.initials: G
authors.lastname: Srinivasa Rao
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar 243 122, UP, India. Electronic address: jkmalik48@gmail.com.
authors.forename: Jitendra K
authors.initials: JK
authors.lastname: Malik
mesh_terms: D000284:Administration, Oral; D016604:Aflatoxin B1 / Q000008:administration & dosage* / Q000633:toxicity; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D059550:Toxicity Tests, Subchronic
chemical_list: D024841:Fluoroquinolones; D000077422:Enrofloxacin; D016604:Aflatoxin B1
keywords: Aflatoxin B(1); Broiler chickens; Enrofloxacin; HPLC assay; Pharmacokinetic disposition; Plasma concentration