title: Proteomic analysis of HIV-1 Gag interacting partners using proximity-dependent biotinylation.
abstract: BACKGROUND
  The human immunodeficiency virus type 1 (HIV-1) Gag polyprotein is necessary and sufficient to assemble non-infectious particles. Given that HIV-1 subverts many host proteins at all stages of its life cycle, it is essential to identify these interactions as potential targets for antiretroviral therapy.
  
  
  RESULTS
  This work demonstrates the use of proximity-dependent biotin identification (BioID) of host proteins and complexes that are proximal to the N-terminal domains of the HIV-1 Gag polyprotein. Two of the hits identified in the BioID screen were validated by immunoprecipation and confirmed the interaction of DDX17 and RPS6 with HIV-1 Gag.
  
  
  CONCLUSIONS
  Our results show that BioID is both a successful and complementary method to screen for nearby interacting proteins of HIV-1 Gag during the replicative cycle in different cell lines.
authors.affiliation: HIV-1 RNA Trafficking Laboratory, Lady Davis Institute at the Jewish General Hospital, Montréal, Québec, H3T 1E2, Canada. valerie.lesage@mail.mcgill.ca.
authors.forename: Valerie
authors.initials: V
authors.lastname: Le Sage
authors.affiliation: HIV-1 RNA Trafficking Laboratory, Lady Davis Institute at the Jewish General Hospital, Montréal, Québec, H3T 1E2, Canada. alessandro.cinti@mail.mcgill.ca.|Department of Medicine, McGill University, Montréal, Québec, H3A 0G4, Canada. alessandro.cinti@mail.mcgill.ca.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Cinti
authors.affiliation: HIV-1 RNA Trafficking Laboratory, Lady Davis Institute at the Jewish General Hospital, Montréal, Québec, H3T 1E2, Canada. fvaliente@med.uchile.cl.|Department of Medicine, McGill University, Montréal, Québec, H3A 0G4, Canada. fvaliente@med.uchile.cl.|Present address: Molecular and Cellular Virology Laboratory, Virology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Independencia, 834100, Santiago, Chile. fvaliente@med.uchile.cl.
authors.forename: Fernando
authors.initials: F
authors.lastname: Valiente-Echeverría
authors.affiliation: HIV-1 RNA Trafficking Laboratory, Lady Davis Institute at the Jewish General Hospital, Montréal, Québec, H3T 1E2, Canada. andrew.mouland@mcgill.ca.|Department of Medicine, McGill University, Montréal, Québec, H3A 0G4, Canada. andrew.mouland@mcgill.ca.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Mouland
mesh_terms: D019921:Biotinylation; D015497:HIV-1 / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*
chemical_list: D011506:Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus