title: Ultrastructural insights into tomato infections caused by three different pathotypes of Pepino mosaic virus and immunolocalization of viral coat proteins.
abstract: This paper presents studies on an ultrastructural analysis of plant tissue infected with different pathotypes of Pepino mosaic virus (PepMV) and the immunolocalization of viral coat proteins. Because the PepMV virus replicates with a high mutation rate and exhibits significant genetic diversity, therefore, isolates of PepMV display a wide range of symptoms on infected plants. In this work, tomato plants of the Beta Lux cultivar were inoculated mechanically with three pathotypes representing the Chilean 2 (CH2) genotype: mild (PepMV-P22), necrotic (PepMV-P19) and yellowing (PepMV-P5-IY). The presence of viral particles in all infected plants in the different compartments of various cell types (i.e. spongy and palisade mesophyll, sieve elements and xylem vessels) was revealed via ultrastructural analyses. For the first time, it was possible to demonstrate the presence of crystalline inclusions, composed of virus-like particles. In the later stage of PepMV infection (14 dpi) various pathotype-dependent changes in the structure of the individual organelles (i.e. mitochondria, chloroplasts) were found. The strongest immunogold labeling of the viral coat proteins was also observed in plants infected by necrotic isolates. A large number of viral coat proteins were marked in the plant conductive elements, both xylem and phloem.
authors.affiliation: Department of Virology and Bacteriology, Institute of Plant Protection - National Research Institute, Wl. Wegorka 20, 60-318 Poznan, Poland.
authors.forename: Julia
authors.initials: J
authors.lastname: Minicka
authors.affiliation: Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences (WULS-SGGW), Nowoursynowska 159, 02-776 Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Otulak
authors.affiliation: Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences (WULS-SGGW), Nowoursynowska 159, 02-776 Warsaw, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Garbaczewska
authors.affiliation: Department of Virology and Bacteriology, Institute of Plant Protection - National Research Institute, Wl. Wegorka 20, 60-318 Poznan, Poland.
authors.forename: Henryk
authors.initials: H
authors.lastname: Pospieszny
authors.affiliation: Department of Virology and Bacteriology, Institute of Plant Protection - National Research Institute, Wl. Wegorka 20, 60-318 Poznan, Poland. Electronic address: beatahasiow@tlen.pl.
authors.forename: Beata
authors.initials: B
authors.lastname: Hasiów-Jaroszewska
mesh_terms: D036022:Capsid Proteins / Q000648:ultrastructure; D002677:Chile; D007150:Immunohistochemistry; D018551:Solanum lycopersicum / Q000166:cytology / Q000648:ultrastructure* / Q000821:virology*; D009029:Mosaic Viruses / Q000737:chemistry / Q000472:pathogenicity / Q000648:ultrastructure; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000648:ultrastructure / Q000821:virology; D017863:Potexvirus / Q000737:chemistry / Q000648:ultrastructure; D014774:Virulence
chemical_list: D036022:Capsid Proteins
keywords: Cytopathology; Immunolocalization; Pepino mosaic virus; Ultrastructure; Virulence