title: Mathematical modeling of enzyme production using Trichoderma harzianum P49P11 and sugarcane bagasse as carbon source.
abstract: A mathematical model to describe the kinetics of enzyme production by the filamentous fungus Trichoderma harzianum P49P11 was developed using a low cost substrate as main carbon source (pretreated sugarcane bagasse). The model describes the cell growth, variation of substrate concentration and production of three kinds of enzymes (cellulases, beta-glucosidase and xylanase) in different sugarcane bagasse concentrations (5; 10; 20; 30; 40 gL(-1)). The 10 gL(-1) concentration was used to validate the model and the other to parameter estimation. The model for enzyme production has terms implicitly representing induction and repression. Substrate variation was represented by a simple degradation rate. The models seem to represent well the kinetics with a good fit for the majority of the assays. Validation results indicate that the models are adequate to represent the kinetics for a biotechnological process.
authors.affiliation: State University of Campinas, Avenida Albert Einstein 500, CEP 13083-852 Campinas, São Paulo, Brazil. Electronic address: lucas26gelain@hotmail.com.
authors.forename: Lucas
authors.initials: L
authors.lastname: Gelain
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory - CTBE, Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, Caixa Postal 6192, CEP 13083-852 Campinas, São Paulo, Brazil.
authors.forename: José Geraldo
authors.initials: JG
authors.lastname: da Cruz Pradella
authors.affiliation: State University of Campinas, Avenida Albert Einstein 500, CEP 13083-852 Campinas, São Paulo, Brazil.
authors.forename: Aline Carvalho
authors.initials: AC
authors.lastname: da Costa
mesh_terms: D001709:Biotechnology / Q000379:methods; D002244:Carbon / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry*; D015233:Models, Statistical; D031786:Saccharum / Q000737:chemistry*; D014242:Trichoderma / Q000737:chemistry*; D001617:beta-Glucosidase / Q000096:biosynthesis*
chemical_list: D002244:Carbon; D002482:Cellulose; C027433:bagasse; D001617:beta-Glucosidase
keywords: Enzyme production; Fermentation process; Mathematical modeling; Trichoderma harzianum