title: Modulatory effects of inosine, guanosine and uridine on lipopolysaccharide-evoked increase in spike-wave discharge activity in Wistar Albino Glaxo/Rijswijk rats.
abstract: We showed previously that the number of spike-wave discharges (SWDs) was increased after intraperitoneal (i.p.) injection of lipopolysaccharide (LPS), inosine (Ino) and muscimol alone whereas i.p. guanosine (Guo), uridine (Urd), bicuculline, theophylline and (+)-5-methyl-10,11-dihydro-5H-dibenzo(a,d)cyclohepten-5,10-imine maleate (MK-801) alone decreased the SWD number in Wistar Albino Glaxo/Rijswijk (WAG/Rij) rats. These drugs may exert their effects on absence epileptic activity mainly via proinflammatory cytokines-evoked increase in cortical excitability (such as LPS), GABAergic system (LPS, Ino, Urd, muscimol and bicuculline), glutamatergic system (LPS, Guo and MK-801) and adenosinergic system (LPS, Ino, Guo, Urd and theophylline). Both GABAergic system and glutamatergic system are involved in the pathomechanism of absence epilepsy, the LPS-evoked increase in absence epileptic activity and the pro- or antiepileptic effects of non-adenosine (non-Ado) nucleosides Ino, Guo and Urd. Moreover, Ino, Guo and Urd have modulatory effects on inflammatory processes. Thus, we investigated whether Ino, Guo and Urd have also modulatory influence on LPS-evoked increase in SWD number using two different concentrations of each nucleoside in WAG/Rij rats. We demonstrated that Ino dose-dependently aggravated whereas Guo and Urd attenuated the LPS-evoked increase in SWD number. Our results suggest that different nucleosides have diverse effects on LPS-induced changes in absence epileptic activity.
authors.affiliation: Department of Zoology, University of West Hungary, Savaria Campus, Szombathely, Károlyi Gáspár tér 4., 9700, Hungary. Electronic address: zskovacs@ttk.nyme.hu.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Kovács
authors.affiliation: Laboratory of Proteomics, Eötvös Loránd University, Budapest, Pázmány Péter sétány 1C, 1117, Hungary; Department of Physiology and Neurobiology, Eötvös Loránd University, Budapest, Pázmány Péter sétány 1C, 1117, Hungary. Electronic address: kakekesi@dec001.geobio.elte.hu.
authors.forename: Katalin A
authors.initials: KA
authors.lastname: Kékesi
authors.affiliation: Laboratory of Proteomics, Eötvös Loránd University, Budapest, Pázmány Péter sétány 1C, 1117, Hungary; MTA-TTK NAP MS Neuroproteomics Research Group, Hungarian Academy of Sciences, Budapest, Magyar tudósok körútja 2., 1117, Hungary. Electronic address: gjuhasz@dec001.geobio.elte.hu.
authors.forename: Gábor
authors.initials: G
authors.lastname: Juhász
authors.affiliation: MTA-ELTE NAP Laboratory of Molecular and Systems Neurobiology, Institute of Biology, Hungarian Academy of Sciences and Eötvös Loránd University, Budapest, Pázmány Péter sétány 1C, 1117, Hungary; Laboratory of Neuromorphology and Human Brain Tissue Bank, Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest,Tűzoltó u. 58., 1094, Hungary. Electronic address: dobolyi.arpad@med.semmelweis-univ.hu.
authors.forename: Arpád
authors.initials: A
authors.lastname: Dobolyi
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D001921:Brain / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004569:Electroencephalography / Q000187:drug effects; D004832:Epilepsy, Absence / Q000139:chemically induced / Q000188:drug therapy / Q000503:physiopathology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D012263:Ribonucleosides / Q000494:pharmacology*
chemical_list: D000927:Anticonvulsants; D008070:Lipopolysaccharides; D012263:Ribonucleosides
keywords: Absence epilepsy; Guanosine; Inosine; LPS; Uridine; WAG/Rij rats