title: Construction and immunogenicity of the recombinant Lactobacillus acidophilus pMG36e-E0-LA-5 of bovine viral diarrhea virus.
abstract: Bovine viral diarrhea/mucosal disease (BVD/MD) is an infectious disease of cattle with a worldwide distribution, creating a substantial economic impact. It is caused by bovine viral diarrhea virus (BVDV). This research was conducted to construct the recombinant Lactobacillus acidophilus (L. acidophilus) pMG36e-E0-LA-5 of BVDV E0 gene and to test its immunogenicity and protective efficacy against BVDV infection in the mice model. The BVDV E0 gene was sub-cloned into the expression vector and then transformed into the L. acidophilus LA-5 strain by electroporation. The recombinant L. acidophilus pMG36e-E0-LA-5 was confirmed by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. The mice were immunized orally with the recombinant L. acidophilus pMG36e-E0-LA-5. The serum IgG antibody and fecal sIgA antibody responses, expression levels of interleukin (IL)-12 (IL-12) and interferon gamma (IFN-γ) were detected respectively. On the 7th day after the last-immunization, the mice were inoculated with BVDV to evaluate the protective efficiency of the recombinant L. acidophilus pMG36e-E0-LA-5. The results showed that the expressed products protein E0 in the L. acidophilus LA-5 resulted in single band of 27kDa by SDS-PAGE and its strong reactivity with BVDV antibody was confirmed by Western blotting. The IgG and sIgA antibodies responses, IL-12 and IFN-γ expression levels in the vaccinated mice with recombinant L. acidophilus pMG36e-E0-LA-5 were significantly higher than those in the control mice. The protective rate of the vaccinated mice against BVDV increased significantly, and a 90.00% protection rate in virulent challenge was observed. These results indicated that the recombinant L. acidophilus pMG36e-E0-LA-5 strain was successfully constructed and it could effectively improve the immune response in mice and might provide protection against BVDV.
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.
authors.forename: Yuelan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.
authors.forename: Lufeng
authors.initials: L
authors.lastname: Jiang
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Cao
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.
authors.forename: Yongzhan
authors.initials: Y
authors.lastname: Bao
authors.affiliation: College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China. Electronic address: qjhqqq@126.com.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qin
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology / Q000517:prevention & control*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D004337:Drug Carriers; D005243:Feces / Q000737:chemistry; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D007779:Lactobacillus acidophilus / Q000235:genetics*; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D044967:Serum / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D004337:Drug Carriers; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; D018664:Interleukin-12; D007371:Interferon-gamma
keywords: Bovine viral diarrhea virus; E0 gene; Immunogenicity; L. acidophilus; Protective efficacy