title: Application of circular dichroism for structural analysis of surface-immobilized cecropin A interacting with lipoteichoic acid.
abstract: The development of biomaterials integrating antimicrobial peptides (AMPs) for improved pathogen detection or use as therapeutic agents requires an understanding of how a peptide may behave once immobilized. Here, we use a combination of circular dichroism and capture assays to assess the structure-function relationship of the cationic amphipathic AMP, cecropin A (cecA), upon interaction with Gram-positive lipoteichoic acids (LTAs). In solution, cecA peptides underwent a change from a largely unstructured conformation in water to structures with significant α-helical content in the presence of both Bacillus subtilis and Staphylococcus aureus LTAs. After surface immobilization, cecA peptides attached by either C- or N-terminus were able to capture both LTAs as well as to undergo conformational changes in the presence of SDS similar to those observed in solution. However, in spite of demonstrated LTA binding activity and the ability to undergo conformational changes (i.e., with SDS), no structural changes were observed when cecA immobilized by its N-terminus was treated with either LTA preparation. On the other hand, cecA immobilized by its C-terminus underwent a conformational change in the presence of S. aureus, but not B. subtilis, LTA. These results indicate that after immobilization recognition of different targets by cationic AMPs may occur by mechanisms quite different from those in solution and that selectivity of these mechanisms is further dependent on the orientation of the immobilized peptide.
authors.affiliation: Center for Biomolecular Science & Engineering, Naval Research Laboratory , 4555 Overlook Avenue, SW, Washington, D.C. 20375, United States.
authors.forename: Stella H
authors.initials: SH
authors.lastname: North
authors.affiliation: Center for Biomolecular Science & Engineering, Naval Research Laboratory , 4555 Overlook Avenue, SW, Washington, D.C. 20375, United States.
authors.forename: Chris R
authors.initials: CR
authors.lastname: Taitt
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D002942:Circular Dichroism / Q000379:methods*; D008070:Lipopolysaccharides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013499:Surface Properties; D013682:Teichoic Acids / Q000737:chemistry*
chemical_list: D023181:Antimicrobial Cationic Peptides; D008070:Lipopolysaccharides; D010455:Peptides; D013682:Teichoic Acids; D012967:Sodium Dodecyl Sulfate; C009900:lipoteichoic acid; C031161:cecropin A