title: A murine model of targeted infusion for intracranial tumors.
abstract: Historically, intra-arterial (IA) drug administration for malignant brain tumors including glioblastoma multiforme (GBM) was performed as an attempt to improve drug delivery. With the advent of percutaneous neuorovascular techniques and modern microcatheters, intracranial drug delivery is readily feasible; however, the question remains whether IA administration is safe and more effective compared to other delivery modalities such as intravenous (IV) or oral administrations. Preclinical large animal models allow for comparisons between treatment routes and to test novel agents, but can be expensive and difficult to generate large numbers and rapid results. Accordingly, we developed a murine model of IA drug delivery for GBM that is reproducible with clear readouts of tumor response and neurotoxicities. Herein, we describe a novel mouse model of IA drug delivery accessing the internal carotid artery to treat ipsilateral implanted GBM tumors that is consistent and reproducible with minimal experience. The intent of establishing this unique platform is to efficiently interrogate targeted anti-tumor agents that may be designed to take advantage of a directed, regional therapy approach for brain tumors.
authors.affiliation: Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, 14263, USA.
authors.forename: Minhyung
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Neuro Oncology, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors.forename: Tara A
authors.initials: TA
authors.lastname: Barone
authors.affiliation: Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Fedtsova
authors.affiliation: Buffalo BioLabs, LLC, Buffalo, NY, USA.
authors.forename: Anatoli
authors.initials: A
authors.lastname: Gleiberman
authors.affiliation: Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, 14263, USA.
authors.forename: Chandler D
authors.initials: CD
authors.lastname: Wilfong
authors.affiliation: Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, 14263, USA.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Alosi
authors.affiliation: Department of Neuro Oncology, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Plunkett
authors.affiliation: Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors.forename: Andrei
authors.initials: A
authors.lastname: Gudkov
authors.affiliation: Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, 14263, USA. joseph.skitzki@roswellpark.org.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Skitzki
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000473:pathology; D002460:Cell Line; D004195:Disease Models, Animal; D016503:Drug Delivery Systems / Q000295:instrumentation; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005909:Glioblastoma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D007261:Infusions, Intra-Arterial; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009460:Neurologic Examination; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D005904:Glial Fibrillary Acidic Protein
keywords: GBM; Glioblastoma multiforme; Infusion; Intra-arterial; Mouse; Regional cancer therapy