title: CrWSKP1, an SKP1-like Gene, Is Involved in the Self-Incompatibility Reaction of "Wuzishatangju" (Citrus reticulata Blanco).
abstract: Plant S-phase kinase-associated protein 1 (SKP1) genes play crucial roles in plant development and differentiation. However, the role of SKP1 in citrus is unclear. Herein, we described a novel SKP1-like gene, designated as CrWSKP1, from "Wuzishatangju" (Citrus reticulata Blanco). The cDNA sequence of CrWSKP1 is 779 base pairs (bp) and contains an open reading frame (ORF) of 477 bp. The genomic sequence of the CrWSKP1 gene is 1296 bp with two exons and one intron. CrWSKP1 has high identity with SKP1-like genes from other plant species within two conserved regions. Approximately 85% of pollen tubes of self-pollinated CrWSKP1 transgenic tobaccos became twisted at four days after self-pollination. Pollen tube numbers of self-pollinated CrWSKP1 transformants entering into ovules were significantly fewer than that of the control. Seed number of self-pollinated CrWSKP1 transformants was significantly reduced. These results suggested that the CrWSKP1 is involved in the self-incompatibility (SI) reaction of "Wuzishatangju".
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. ziyue7766@163.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. hxmrain@163.com.|Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Tropical Crop Bioscience and Biotechnology, Ministry of Agriculture, Haikou 571101, China. hxmrain@163.com.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Miao
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. pinganma@126.com.
authors.forename: Yuewen
authors.initials: Y
authors.lastname: Ma
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. wxiaolu0217@163.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. guibing@scau.edu.cn.
authors.forename: Guibing
authors.initials: G
authors.lastname: Hu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. qyh6k@163.com.
authors.forename: Zixing
authors.initials: Z
authors.lastname: Ye
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. zhaojietang@gmail.com.
authors.forename: Jietang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. qinyh@scau.edu.cn.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Qin
mesh_terms: D000595:Amino Acid Sequence; D002957:Citrus / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D030821:Plants, Genetically Modified; D054817:Pollination; D044786:S-Phase Kinase-Associated Proteins / Q000235:genetics*; D060436:Self-Incompatibility in Flowering Plants / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000235:genetics
chemical_list: D044786:S-Phase Kinase-Associated Proteins
keywords: Citrus reticulata Blanco; SKP1; genetic transformation; self-incompatibility