title: Digital phantoms generated by spectral and spatial light modulators.
abstract: A hyperspectral image projector (HIP) based on liquid crystal on silicon spatial light modulators is explained and demonstrated to generate data cubes. The HIP-constructed data cubes are three-dimensional images of the spatial distribution of spectrally resolved abundances of intracellular light-absorbing oxyhemoglobin molecules in single erythrocytes. Spectrally and spatially resolved image data indistinguishable from the real scene may be used as standard data cubes, so-called digital phantoms, to calibrate image sensors and validate image analysis algorithms for their measurement quality, performance consistency, and interlaboratory comparisons for quantitative biomedical imaging applications.
authors.affiliation: National Institute of Standards and Technology, Quantum Electronics and Photonics Division, 325 Broadway Street, Boulder, Colorado 80305, United States.
authors.forename: Bonghwan
authors.initials: B
authors.lastname: Chon
authors.affiliation: National Institutes of Health, Laboratory of Malaria and Vector Research, 9000 Rockville Pike, Bethesda, Maryland 20892, United States.
authors.forename: Fuyuki
authors.initials: F
authors.lastname: Tokumasu
authors.affiliation: National Institute of Standards and Technology, Quantum Electronics and Photonics Division, 325 Broadway Street, Boulder, Colorado 80305, United States.
authors.forename: Ji Youn
authors.initials: JY
authors.lastname: Lee
authors.affiliation: National Institute of Standards and Technology, Sensor Science Division, 100 Bureau Drive, Gaithersburg, Maryland 20899, United States.
authors.forename: David W
authors.initials: DW
authors.lastname: Allen
authors.affiliation: National Institute of Standards and Technology, Sensor Science Division, 100 Bureau Drive, Gaithersburg, Maryland 20899, United States.
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Rice
authors.affiliation: National Institute of Standards and Technology, Quantum Electronics and Photonics Division, 325 Broadway Street, Boulder, Colorado 80305, United States.
authors.forename: Jeeseong
authors.initials: J
authors.lastname: Hwang
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D006801:Humans; D008589:Meningococcal Infections / Q000276:immunology*; D009345:Neisseria meningitidis / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines