title: Flavonoids isolated from Tridax procumbens (TPF) inhibit osteoclasts differentiation and bone resorption.
abstract: BACKGROUND
  The Tridax procumbens flavonoids (TPF), are well known for their medicinal properties among local natives. The TPF are traditionally used for dropsy, anaemia, arthritis, gout, asthma, ulcer, piles, and urinary problems. It also used in treating gastric problems, body pain, and rheumatic pains of joints. The TPF have been reported to increase osteogenic functioning in mesenchymal stem cells. However, their effects on osteoclastogenesis remain unclear. The TPF isolated from T. procumbens and investigated the effects of the TPF inhibit on osteoclast differentiation and bone resorption activities using primary osteoclastic cells. Osteoclast formation was assessed by counting the number of tartrate resistant acid phosphatase (TRAP) positive multinucleated cells and by measuring both TRAP activities.
  
  
  RESULTS
  The TPF significantly suppressed the RANKL-induced differentiation of osteoclasts and the formation of pits in primary osteoclastic cells. The TPF also decreased the expression of mRNAs related to osteoclast differentiation, including Trap, Cathepsin K, Mmp-9, and Mmp-13 in primary osteoclastic cells. The treatment of primary osteoclastic cells with the TPF decreased Cathepsin K, Mmp-9, and Mmp-13 proteins expression in primary osteoclastic cells.
  
  
  CONCLUSIONS
  These results indicated that TPF inhibit osteoclastogenesis and pits formation activities. Our results suggest that the TPF could be a potential anti-bone resorptic agent to treat patients with bone loss-associated diseases such as osteoporosis.
authors.affiliation: Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh. mssohel@yahoo.com.
authors.forename: Md Abdullah
authors.initials: MA
authors.lastname: Al Mamun
authors.affiliation: Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh. kamu_islamm@yahoo.com.
authors.forename: Kamrul
authors.initials: K
authors.lastname: Islam
authors.affiliation: Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh. jalambioru@yahoo.com.
authors.forename: Md Jahangir
authors.initials: MJ
authors.lastname: Alam
authors.affiliation: Department of Anthropology, Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh. akhatun6july@yahoo.com.
authors.forename: Amina
authors.initials: A
authors.lastname: Khatun
authors.affiliation: Department of Applied Nutrition and Food Technology, Islamic University, Kustia, 7003, Bangladesh. alam.anft@gmail.com.
authors.forename: M Masihul
authors.initials: MM
authors.lastname: Alam
authors.affiliation: Department of Pharmacy, Rajshahi University, Rajshahi, 6205, Bangladesh. alimalbari347@ru.ac.bd.
authors.forename: Md Abdul Alim
authors.initials: MA
authors.lastname: Al-Bari
authors.affiliation: Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh. mja.gebsust@yahoo.com.
authors.forename: Md Jahangir
authors.initials: MJ
authors.lastname: Alam
mesh_terms: D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D001862:Bone Resorption; D002454:Cell Differentiation / Q000187:drug effects*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010010:Osteoclasts / Q000187:drug effects*; D012333:RNA, Messenger; D000071681:Tartrate-Resistant Acid Phosphatase / Q000187:drug effects
chemical_list: D005419:Flavonoids; D012333:RNA, Messenger; D000071681:Tartrate-Resistant Acid Phosphatase