title: Development of a Specific Latex Agglutination Test to Detect Antibodies of Enterovirus 71.
abstract: A latex agglutination test (LAT) was developed for the rapid detection of antibodies against the VP1 or VP1 proteins of Enterovirus 71 (EV71). The proteins of interest including prokaryotically expressed VP1 and two strains of anti-VP1 monoclonal antibody (McAb) against EV71 were covalently linked to carboxylated latex using ethyl-dimethyl-amino-propyl carbodiimide (EDC) to prepare sensitized latex beads. LAT was evaluated by an enzyme-linked immunosorbent assay (ELISA) as a reference test. The VP1-LAT showed a sensitivity of 87.0%, specificity of 88.9%, and an agreement ratio of 90.0% in detecting VP1 in 100 serum samples from experimentally infected mice, whereas these values were 86.8, 96.7, and 93.3%, respectively, for 608 clinical human serum samples. The VP1-LAT has advantages over other assays in terms of low cost, rapidity, chemical stability, high sensitivity, repeatability, and specificity. The LAT established in the present study is a rapid and simple test suitable for field monitoring of antibodies against VP1-EV71.
authors.affiliation: Shaoxing People's Hospital, Shaoxing Hospital of Zhejiang University, Shaoxing, PR China; Shaoxing Centre for Disease Control and Prevention, Shaoxing, Zhejiang Province, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Qin
authors.affiliation: Medical College of Shaoxing University, Shaoxing, PR China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute for Applied Nephrology (Interneph), Fuchserde 7, DE-52066, Aachen, Germany.
authors.forename: Wenhao
authors.initials: W
authors.lastname: Xie
authors.affiliation: Medical College of Shaoxing University, Shaoxing, PR China.
authors.forename: Xuehong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Shaoxing Centre for Disease Control and Prevention, Shaoxing, Zhejiang Province, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: He
authors.affiliation: Shaoxing Centre for Disease Control and Prevention, Shaoxing, Zhejiang Province, PR China.
authors.forename: Jinkun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Shaoxing People's Hospital, Shaoxing Hospital of Zhejiang University, Shaoxing, PR China. Electronic address: qinbo0809@163.com.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Dong
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood*; D036022:Capsid Proteins / Q000276:immunology*; D029821:Enterovirus A, Human / Q000276:immunology*; D004769:Enterovirus Infections / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D051379:Mice; D008863:Microspheres; D011817:Rabbits; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins
keywords: Enterovirus 71; Ethyl-dimethyl-amino-propyl carbodiimide; Hand-foot-and-mouth disease; Homogeneity; Latex agglutination test