title: Decision-making microRNAs (miR-124, -133a/b, -34a and -134) in patients with occluded target vessel in acute coronary syndrome.
abstract: BACKGROUND
  Coronary artery occlusion does not always manifest with ST-elevation, and some patients can have patent coronary vessel.
  
  
  OBJECTIVE
  We evaluated circulating microRNA (miRNA) profiles to discriminate subjects with infarct-related artery (IRA) occlusion.
  
  
  RESULTS
  Patients (n = 43) with uncomplicated acute coronary syndrome and positive troponins were classified with respect to patent vs. occluded IRA or ST-elevation vs. non-ST elevation MI (STEMI vs. NSTEMI). Expression levels of serum miRNAs (miR-1, -16, -34a, -122, -124, -208b, -133a/b, -375, and -499) were analysed. Out of 16 STEMI and 27 NSTEMI patients, IRA occlusion was noted in 12 and 15 patients, respectively. The remaining four STEMI and 12 NSTEMI patients had patent IRA. STEMI patients had higher troponin T levels and a 3.83-fold higher miR-134 expression (p < 0.025). Patients with the occluded vs. patent IRA had higher levels of miR-133a (fold change: 7.00), miR-133b (4.57), miR-34a (5.50), miR-124 (2.55), and miR-134 (3.45) but no difference in troponin T levels. Receiver operator characteristic analysis identified decision-making miRNAs in occluded vessels: miR-124 (AUC: 0.787, p < 0.001), miR-133b (AUC: 0.704, p = 0.006), and miR-134 (AUC: 0.686, p = 0.016). With respect to STEMI, only miR-134 showed a discriminating value (AUC: 0.725, p = 0.002).
  
  
  CONCLUSIONS
  The degree of IRA occlusion determines circulating miRNA expression, and specific miRNAs may be useful in indicating patients requiring urgent coronary revascularisation.
authors.affiliation: Oddział Kardiologii Inwazyjnej, Specjalistyczny Szpital im. Edwarda Szczeklika, Tarnów. jgacon@o2.pl.
authors.forename: Jacek
authors.initials: J
authors.lastname: Gacoń
authors.forename: Anna
authors.initials: A
authors.lastname: Kabłak-Ziembicka
authors.forename: Ewa
authors.initials: E
authors.lastname: Stępień
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Enguita
authors.forename: Izabela
authors.initials: I
authors.lastname: Karch
authors.forename: Bogusław
authors.initials: B
authors.lastname: Derlaga
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Żmudka
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Przewłocki
mesh_terms: D054058:Acute Coronary Syndrome / Q000097:blood / Q000209:etiology*; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D054059:Coronary Occlusion / Q000097:blood / Q000150:complications* / Q000175:diagnosis; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D012372:ROC Curve; D020107:Troponin T / Q000097:blood
chemical_list: D015415:Biomarkers; C509207:MIRN124 microRNA, human; C548234:MIRN133 microRNA, human; C569273:MIRN134 microRNA, human; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D020107:Troponin T
keywords: NSTEMI; STEMI; acute coronary syndrome; infarct-related artery; mRNA; occluded