title: 1H, 13C, 15N resonance assignments of the extracellular loop 1 domain (ECL1) of Streptococcus pneumoniae D39 FtsX, an essential cell division protein.
abstract: FtsX is an integral membrane protein from Streptococcus pneumoniae (pneumococcus) that harbors an extracellular loop 1 domain (FtsX(Spn)ECL1) that interacts with PcsB, an peptidoglycan hydrolase that is essential for cell growth and division. Here, we report nearly complete backbone and side chain resonance assignments and a secondary structural analysis of FtsX(Spn)ECL1 (residues 47-168 of FtsX) as first steps toward structure determination of FtsX(Spn)ECL1.
authors.affiliation: Department of Chemistry, Indiana University, 212 S. Hawthorne Drive, Bloomington, IN, 47405-7102, USA.
authors.forename: Yue
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Biology, Indiana University, Bloomington, IN, 47405, USA.
authors.forename: Kevin E
authors.initials: KE
authors.lastname: Bruce
authors.affiliation: Department of Biology, Indiana University, Bloomington, IN, 47405, USA.
authors.forename: Britta
authors.initials: B
authors.lastname: Rued
authors.affiliation: Department of Biology, Indiana University, Bloomington, IN, 47405, USA.
authors.forename: Malcolm E
authors.initials: ME
authors.lastname: Winkler
authors.affiliation: Department of Chemistry, Indiana University, 212 S. Hawthorne Drive, Bloomington, IN, 47405-7102, USA. giedroc@indiana.edu.
authors.forename: David P
authors.initials: DP
authors.lastname: Giedroc
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D002247:Carbon Isotopes; D018797:Cell Cycle Proteins / Q000737:chemistry*; D002455:Cell Division; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D000072417:Protein Domains; D013296:Streptococcus pneumoniae / Q000166:cytology* / Q000378:metabolism*; D014316:Tritium
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D018797:Cell Cycle Proteins; C102286:FtsX protein, bacteria; D009587:Nitrogen Isotopes; D014316:Tritium
keywords: ABC transporter; Allostery; Bacterial cell wall; Divisome; Extracellular signaling; Peptidoglycan hydrolysis