title: TMPyP4-regulated cell proliferation and apoptosis through the Wnt/β-catenin signaling pathway in SW480 cells.
abstract: BACKGROUND
  The aim of this study was to investigate the potential effects of the 5, 10, 15, 20-tetrakis (1-methylpyridinium-4-yl) porphyrin (TMPyP4) on the proliferation and apoptosis of SW480 cells and the underlying mechanisms by which TMPyP4 exerted its actions.
  
  
  METHODS
  After treated with different doses of TMPyP4, cell viability was determined by MTT method, the apoptosis was observed by flow cytometry (FCM) and the expression of Wnt, GSK-3β, β-catenin and cyclinD1 was measured by RT-PCR and Western blot analysis.
  
  
  RESULTS
  The analysis revealed that TMPyP4 potently suppressed cell viability and induced the apoptosis of SW480 cells in a dose-dependent manner. In addition, the downregulation of Wnt, β-catenin and cyclinD1 expression levels was detected in TMPyP4-treated SW480 cells. However, followed by the block of Wnt signaling pathway using siRNA methods, the effects of TMPyP4 on proliferation and apoptosis of SW480 cells were significantly reduced.
  
  
  CONCLUSIONS
  It indicates that the TMPyP4-inhibited proliferation and -induced apoptosis in SW480 cells was accompanied by the suppression of Wnt/β-catenin signaling pathway. Therefore, TMPyP4 may represent a potential therapeutic method for the treatment of colon carcinoma.
authors.affiliation: a Department of Biochemistry & Molecular Biology Full Affiliation , Shanxi Medical University , Taiyuan , PR China .|b Department of Biochemistry & Molecular Biology Full Affiliation , Changzhi Medical College , Changzhi , PR China , and.
authors.forename: Yi-Qiang
authors.initials: YQ
authors.lastname: Zhang
authors.affiliation: a Department of Biochemistry & Molecular Biology Full Affiliation , Shanxi Medical University , Taiyuan , PR China .
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Zhang
authors.affiliation: a Department of Biochemistry & Molecular Biology Full Affiliation , Shanxi Medical University , Taiyuan , PR China .
authors.forename: Jun
authors.initials: J
authors.lastname: Xie
authors.affiliation: a Department of Biochemistry & Molecular Biology Full Affiliation , Shanxi Medical University , Taiyuan , PR China .
authors.forename: Mei-Ning
authors.initials: MN
authors.lastname: Li
authors.affiliation: a Department of Biochemistry & Molecular Biology Full Affiliation , Shanxi Medical University , Taiyuan , PR China .
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Liu
authors.affiliation: a Department of Biochemistry & Molecular Biology Full Affiliation , Shanxi Medical University , Taiyuan , PR China .
authors.forename: Jin-Yan
authors.initials: JY
authors.lastname: Shen
authors.affiliation: c Department of Nephrology , Changzhi Medical College Affiliated Heji Hospital , Changzhi , PR China.
authors.forename: Shuai-Shuai
authors.initials: SS
authors.lastname: Shi
authors.affiliation: a Department of Biochemistry & Molecular Biology Full Affiliation , Shanxi Medical University , Taiyuan , PR China .
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Lan
authors.affiliation: a Department of Biochemistry & Molecular Biology Full Affiliation , Shanxi Medical University , Taiyuan , PR China .
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.affiliation: a Department of Biochemistry & Molecular Biology Full Affiliation , Shanxi Medical University , Taiyuan , PR China .
authors.forename: Niu-Liang
authors.initials: NL
authors.lastname: Cheng
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D011166:Porphyrins / Q000008:administration & dosage*; D034741:RNA, Small Interfering / Q000235:genetics; D060449:Wnt Signaling Pathway / Q000187:drug effects
chemical_list: D011166:Porphyrins; D034741:RNA, Small Interfering; C021096:tetra(4-N-methylpyridyl)porphine
keywords: Apoptosis; SW480 cells; TMPyP4; Wnt/β-catenin; proliferation