title: Alteration in Nuclear Factor-KappaB Pathway and Functionality of Estrogen via Receptors Promote Neuroinflammation in Frontal Cortex after 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine Treatment.
abstract: The MPTP mediated neurodegeneration in substantia nigra has been well studied, but not the status of frontal cortex. The novelty of the present study is to explore the sex difference of frontal cortex during MPTP intoxication and to investigate the role of estrogen and its receptors in presence of glial cells in a time chase experiment; to identify which pathway of NF-kappaB exist to proceed the neuroinflammation; to investigate the estrogen binding with its nuclear or cytosolic receptors and whether any direct relation exists between estrogen receptor (ER) -beta and NF-kappaB molecules p65 and RelB. The progression of neurodegeneration occurred with the association of glial cells and functional (via its nuclear and cytosolic receptors) estrogen level. Both the canonical and/or non canonical pathways of NF-kappaB exist in frontal cortex of both the sexes after MPTP treatment. The homodimeric or heterodimeric form of ER-beta binds with NF-kappaB molecules p65 and RelB differently, but the canonical or non canonical pathways of NF-kappaB molecules could not be stopped or may be promoted. The changes in the molecular and cellular pattern in frontal cortex of both sexes during MPTP intoxication depends on the estrogen function via its nuclear or cytosolic estrogen receptors.
authors.affiliation: Immunology Lab, Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road. Kolkata-700019, India.
authors.forename: Soham
authors.initials: S
authors.lastname: Mitra
authors.affiliation: Immunology Lab, Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road. Kolkata-700019, India.
authors.forename: Nabanita
authors.initials: N
authors.lastname: Ghosh
authors.affiliation: Department of Physiology, University of Calcutta, 92, Acharya Prafulla Chandra Road, Kolkata-700009, India.
authors.forename: Priyobrata
authors.initials: P
authors.lastname: Sinha
authors.affiliation: Department of Physiology, University of Calcutta, 92, Acharya Prafulla Chandra Road, Kolkata-700009, India.
authors.forename: Nilkanta
authors.initials: N
authors.lastname: Chakrabarti
authors.affiliation: Immunology Lab, Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road. Kolkata-700019, India.
authors.forename: Arindam
authors.initials: A
authors.lastname: Bhattacharyya
mesh_terms: D000818:Animals; D001141:Aromatase / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D047628:Estrogen Receptor alpha / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000378:metabolism*; D004967:Estrogens / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D005625:Frontal Lobe / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D007249:Inflammation / Q000209:etiology; D020267:MPTP Poisoning; D008297:Male; D051379:Mice; D008840:Microfilament Proteins / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D011485:Protein Binding; D013629:Tamoxifen / Q000494:pharmacology; D051996:Transcription Factor RelA / Q000378:metabolism; D051841:Transcription Factor RelB / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism
chemical_list: C491049:Aif1 protein, mouse; D002135:Calcium-Binding Proteins; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D005904:Glial Fibrillary Acidic Protein; D008840:Microfilament Proteins; D016328:NF-kappa B; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D013629:Tamoxifen; D051841:Transcription Factor RelB; D001141:Aromatase