title: A label-free, multiplex competitive assay for small molecule pollutants.
abstract: Understanding the amount of exposure individuals have had to common chemical pollutants critically requires the ability to detect those compounds in a simple, sensitive, and specific manner. Doing so using label-free biosensor technology has proven challenging, however, given the small molecular weight of many pollutants of interest. To address this issue, we report the development of a pollutant microarray based on the label-free arrayed imaging reflectometry (AIR) detection platform. The sensor is able to detect three common environmental contaminants (benzo[a]pyrene, bisphenol A, and acrolein) in human serum via a competitive binding scheme.
authors.affiliation: Adarza BioSystems, 150 Lucius Gordon Drive, Suite 110, West Henrietta, NY 14586, USA. Electronic address: Jared_carter@adarzabio.com.
authors.forename: Jared A
authors.initials: JA
authors.lastname: Carter
authors.affiliation: Adarza BioSystems, 150 Lucius Gordon Drive, Suite 110, West Henrietta, NY 14586, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Triplett
authors.affiliation: Adarza BioSystems, 150 Lucius Gordon Drive, Suite 110, West Henrietta, NY 14586, USA.
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Striemer
authors.affiliation: Department of Dermatology, University of Rochester, 601 Elmwood Avenue Box 697, Rochester, NY 14642, USA.
authors.forename: Benjamin L
authors.initials: BL
authors.lastname: Miller
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D045424:Complex Mixtures / Q000032:analysis* / Q000737:chemistry; D004785:Environmental Pollutants / Q000032:analysis* / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D010783:Photometry / Q000295:instrumentation*; D013194:Staining and Labeling
chemical_list: D045424:Complex Mixtures; D004785:Environmental Pollutants
keywords: Label-Free; Multiplex; Pollutants; Small-molecule; Toxicants