title: Nanopore Diameters Tune Strain in Extruded Fibronectin Fibers.
abstract: Fibronectin is present in the extracellular matrix and can be assembled into nanofibers in vivo by undergoing conformational changes. Here, we present a novel approach to prepare fibronectin nanofibers under physiological conditions using an extrusion approach through nanoporous aluminum oxide membranes. This one-step process can prepare nanofiber bundles up to a millimeter in length and with uniform fiber diameters in the nanometer range. Most importantly, by using different pore diameters and protein concentrations in the extrusion process, we could induce varying lasting structural changes in the fibers, which were monitored by Förster resonance energy transfer and should impose different physiological functions.
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems , Heisenbergstraße 3, D-70569 Stuttgart, Germany.|Department of Biophysical Chemistry, University of Heidelberg , INF 253, D-69120 Heidelberg, Germany.|Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Science , Tehran 1417614411, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Raoufi
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems , Heisenbergstraße 3, D-70569 Stuttgart, Germany.|Department of Biophysical Chemistry, University of Heidelberg , INF 253, D-69120 Heidelberg, Germany.
authors.forename: Tamal
authors.initials: T
authors.lastname: Das
authors.affiliation: Laboratory of Applied Mechanobiology, Department of Health Sciences and Technology ETH Zurich , Vladimir-Prelog Weg 4 (HCI F443), CH-8093 Zurich, Switzerland.
authors.forename: Ingmar
authors.initials: I
authors.lastname: Schoen
authors.affiliation: Laboratory of Applied Mechanobiology, Department of Health Sciences and Technology ETH Zurich , Vladimir-Prelog Weg 4 (HCI F443), CH-8093 Zurich, Switzerland.
authors.forename: Viola
authors.initials: V
authors.lastname: Vogel
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems , Heisenbergstraße 3, D-70569 Stuttgart, Germany.|Department of Biophysical Chemistry, University of Heidelberg , INF 253, D-69120 Heidelberg, Germany.
authors.forename: Dorothea
authors.initials: D
authors.lastname: Brüggemann
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems , Heisenbergstraße 3, D-70569 Stuttgart, Germany.|Department of Biophysical Chemistry, University of Heidelberg , INF 253, D-69120 Heidelberg, Germany.
authors.forename: Joachim P
authors.initials: JP
authors.lastname: Spatz
mesh_terms: D005353:Fibronectins / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D008855:Microscopy, Electron, Scanning; D058608:Nanopores
chemical_list: D005353:Fibronectins
keywords: AAO template; FRET; conformational changes; extrusion; protein nanofiber