title: Characterization and Insights Into the Nano Liposomal Magnetic Gene Vector Used for Cell Co-Transfection.
abstract: The development of magnetofection technology has brought a promising method for gene delivery. Here, we develop a novel liposomal magnetofection system, consisted of magnetic nanoparticle and liposome through molecular assembly, was applied to introduce double genes into porcin somatic cells with high co-transfection efficiency. The performace of liposomal magnetic gene nanovectors has been evaluated by involving the micro morphology, diameters distribution, zeta potentials and the capacity of loading DNA molecules. The assembly way among magnetic gene nanovectors and DNA molecules was investigated by atomic force microscopy. Liposomal nano magnetic gene vectors complexes displayed nanoscale assembly and formed compact "fishing-net structure" after combining with plasmid DNA, which is favorable to enhance the loading capacity of DNA molecules.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Chen
authors.forename: Haixin
authors.initials: H
authors.lastname: Cui
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhao
authors.forename: Jinhui
authors.initials: J
authors.lastname: Cui
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Chaojiao
authors.initials: C
authors.lastname: Sun
authors.forename: Bo
authors.initials: B
authors.lastname: Cui
authors.forename: Feng
authors.initials: F
authors.lastname: Lei
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D004058:Diffusion / Q000528:radiation effects; D007668:Kidney / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D008081:Liposomes / Q000737:chemistry*; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D008422:Materials Testing; D053769:Nanocapsules / Q000737:chemistry / Q000528:radiation effects / Q000648:ultrastructure; D013552:Swine; D014162:Transfection / Q000379:methods*
chemical_list: D008081:Liposomes; D058185:Magnetite Nanoparticles; D053769:Nanocapsules; D004247:DNA