title: The application of HPLC ESI MS in the investigation of the flavonoids and flavonoid glycosides of a Caribbean Lamiaceae plant with potential for bioaccumulation.
abstract: As part of an exchange technology program between the government of Barbados and Environment Canada, methanolic and aqueous extracts from the flavonoid-rich Lamiaceae family were characterized using negative-ion electrospray mass spectrometry. The species investigated is part of the Caribbean Pharmacopoeia, and is used for a variety of health issues, including colds, flu, diabetes, and hypertension. The extracts were investigated for structural elucidation of phenolics, identification of chemical taxonomic profile, and evidence of bio-accumulator potential. The methanolic and aqueous leaf extracts of Plectranthus amboinicus yielded rosmarinic acid, ladanein, cirsimaritin, and other methoxylated flavonoids. This genus also shows a tendency to form conjugates with monosaccharides, including glucose, galactose, and rhamnose. The aqueous extract yielded four isomeric rhamnosides. The formation of conjugates by Plectranthus amboinicus is thus evidence of high bioaccumulator significance.
authors.affiliation: a Departments of Chemistry and Environmental Sciences, Barbados Community College , St. Michael , Barbados.
authors.forename: Sonia R
authors.initials: SR
authors.lastname: Peter
authors.affiliation: b Water Science and Technology Directorate, Aquatic Contaminants Research Division, Environment Canada , Saskatoon, Saskatchewan , Canada.
authors.forename: Kerry M
authors.initials: KM
authors.lastname: Peru
authors.affiliation: b Water Science and Technology Directorate, Aquatic Contaminants Research Division, Environment Canada , Saskatoon, Saskatchewan , Canada.
authors.forename: Brian
authors.initials: B
authors.lastname: Fahlman
authors.affiliation: c Environmental Systems Engineering, University of Regina , Regina, Saskatchewan , Canada.
authors.forename: Dena W
authors.initials: DW
authors.lastname: McMartin
authors.affiliation: b Water Science and Technology Directorate, Aquatic Contaminants Research Division, Environment Canada , Saskatoon, Saskatchewan , Canada.
authors.forename: John V
authors.initials: JV
authors.lastname: Headley
mesh_terms: D001460:Barbados; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D006027:Glycosides / Q000032:analysis / Q000737:chemistry; D007536:Isomerism; D019686:Lamiaceae / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D012210:Rhamnose / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D005419:Flavonoids; D006027:Glycosides; D010636:Phenols; D010936:Plant Extracts; D012210:Rhamnose
keywords: Flavonoidglycosides; mass spectrometry; methoxyflavonoids