title: In vitro probiotic characterization of Lactobacillus strains from fermented radish and their anti-adherence activity against enteric pathogens.
abstract: In this study, we evaluated the probiotic properties of Lactobacillus plantarum, Lactobacillus pentosus, and Lactobacillus fermentum strains isolated from fermented radish. All the strains survived the simulated oro-gastrointestinal transit condition and showed significantly higher adherence to Caco-2 cells compared with the probiotic strain Lactobacillus rhamnosus GG. The strains showed broad-spectrum antimicrobial activity, autoaggregation, and coaggregation capacity with pathogens. Furthermore, the Lactobacillus strains inhibited the adherence of Yersinia enterocolitica subsp. enterocolitica, Shigella boydii, and Salmonella choleraesuis to the Caco-2 cell line. The strains possessed bile salt hydrolase activity and their cholesterol-lowering activity in vitro was above 50% in the presence of bile. Strains of L. plantarum and L. pentosus possessed the plantaricin-encoding plnEF gene. In addition, the Lactobacillus strains maintained about 80% cell viability after freeze-drying in the presence of a combination of 5% skim milk and 5% maltodextrin as cryoprotectant, and 70% recovery of cell viability was observed in the absence of any cryoprotectant.
authors.affiliation: a Center for Nutraceutical and Pharmaceutical Materials, College of Natural Science, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do 449-728, South Korea.|b Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do 449-728, South Korea.
authors.forename: Karthiyaini
authors.initials: K
authors.lastname: Damodharan
authors.affiliation: a Center for Nutraceutical and Pharmaceutical Materials, College of Natural Science, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do 449-728, South Korea.|c Interdisciplinary Program of Biomodulation, College of Natural Science, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do 449-728, South Korea.
authors.forename: Sasikumar Arunachalam
authors.initials: SA
authors.lastname: Palaniyandi
authors.affiliation: a Center for Nutraceutical and Pharmaceutical Materials, College of Natural Science, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do 449-728, South Korea.|c Interdisciplinary Program of Biomodulation, College of Natural Science, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do 449-728, South Korea.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Yang
authors.affiliation: a Center for Nutraceutical and Pharmaceutical Materials, College of Natural Science, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do 449-728, South Korea.|b Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do 449-728, South Korea.
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Suh
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry; D001422:Bacterial Adhesion; D001430:Bacteriocins / Q000235:genetics; D001646:Bile; D001647:Bile Acids and Salts; D018938:Caco-2 Cells; D003470:Culture Media; D004353:Drug Evaluation, Preclinical; D005285:Fermentation; D005612:Freeze Drying; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D007778:Lactobacillus / Q000378:metabolism*; D052197:Limosilactobacillus fermentum; D048191:Lactiplantibacillus plantarum; D008826:Microbial Sensitivity Tests; D008892:Milk; D010802:Phylogeny; D011134:Polysaccharides / Q000737:chemistry; D019936:Probiotics / Q000737:chemistry*; D011498:Protein Precursors / Q000235:genetics; D031224:Raphanus / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D000890:Anti-Infective Agents; D001430:Bacteriocins; D001647:Bile Acids and Salts; D003470:Culture Media; D011134:Polysaccharides; D011498:Protein Precursors; C407314:plnA protein, Lactobacillus plantarum; D019344:Lactic Acid; C008315:maltodextrin
keywords: antagoniste de pathogènes; antimicrobial; antimicrobien; bactéries lactiques; cholesterol-lowering; lactic acid bacteria; pathogen-antagonistic; potentiel probiotique; probiotic potential; réducteur de cholestérol