title: Rapid SERS monitoring of lipid-peroxidation-derived protein modifications in cells using photonic crystal fiber sensor.
abstract: We proposed a side channel photonic crystal fiber (SC-PCF) based Surface enhanced Raman spectroscopy (SERS) platform which is able to accurately monitor lipid peroxidation derived protein modifications in cells. This platform incorporates linoleamide alkyne (LAA), which is oxidized and subsequently modifies proteins in cells with alkyne functional group upon lipid peroxidation. By loading the side channel of SC-PCF with a mixture of gold nanoparticles and LAA treated cells, and subsequently measuring the interference-free alkyne Raman peak from these proteins in cells, strong SERS signal was obtained. The platform provides a method for the rapid monitoring of lipid peroxidation derived protein modification in cells.
authors.affiliation: School of Electrical and Electronic Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798.|Bio-Optical Imaging Group, Singapore Bioimaging Consortium (SBIC), Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, Singapore, 138667.
authors.forename: Tianxun
authors.initials: T
authors.lastname: Gong
authors.affiliation: School of Electrical and Electronic Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798.|CINTRA CNRS/NTU/THALES, Research Techno Plaza, 50 Nanyang Drive, Singapore, 637553.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Bio-Optical Imaging Group, Singapore Bioimaging Consortium (SBIC), Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, Singapore, 138667.
authors.forename: Kien Voon
authors.initials: KV
authors.lastname: Kong
authors.affiliation: Bio-Optical Imaging Group, Singapore Bioimaging Consortium (SBIC), Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, Singapore, 138667.
authors.forename: Douglas
authors.initials: D
authors.lastname: Goh
authors.affiliation: School of Electrical and Electronic Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798.|CINTRA CNRS/NTU/THALES, Research Techno Plaza, 50 Nanyang Drive, Singapore, 637553.
authors.forename: Cui
authors.initials: C
authors.lastname: Ying
authors.affiliation: Xlim research institute, UMR 7252, CNRS/University of Limoges, 123 Avenue Albert Thomas, 87060, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Auguste
authors.affiliation: School of Electrical and Electronic Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798.|CINTRA CNRS/NTU/THALES, Research Techno Plaza, 50 Nanyang Drive, Singapore, 637553.
authors.forename: Perry Ping
authors.initials: PP
authors.lastname: Shum
authors.affiliation: School of Electrical and Electronic Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798.
authors.forename: Lei
authors.initials: L
authors.lastname: Wei
authors.affiliation: Xlim research institute, UMR 7252, CNRS/University of Limoges, 123 Avenue Albert Thomas, 87060, France.
authors.forename: Georges
authors.initials: G
authors.lastname: Humbert
authors.affiliation: School of Electrical and Electronic Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798. ktyong@ntu.edu.sg.
authors.forename: Ken-Tye
authors.initials: KT
authors.lastname: Yong
authors.affiliation: Bio-Optical Imaging Group, Singapore Bioimaging Consortium (SBIC), Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, Singapore, 138667. malini_olivo@sbic.a-star.edu.sg.|School of Physics, National University of Ireland, Galway, Ireland. malini_olivo@sbic.a-star.edu.sg.
authors.forename: Malini
authors.initials: M
authors.lastname: Olivo
mesh_terms: D001555:Benzene Derivatives / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D008041:Linoleic Acids / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D055100:Optical Fibers; D017785:Photons; D011506:Proteins / Q000378:metabolism*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D013997:Time Factors
chemical_list: D001555:Benzene Derivatives; D008041:Linoleic Acids; D011506:Proteins; C420162:linoleamide; C007164:cumene hydroperoxide
keywords: HepG2 cell; Surface enhanced Raman spectroscopy; gold nanoparticles; linoleamide alkyne; lipid peroxidation; side channel PCF