title: Co-localization of growth QTL with differentially expressed candidate genes in rainbow trout.
abstract: We tested whether genes differentially expressed between large and small rainbow trout co-localized with familial QTL regions for body size. Eleven chromosomes, known from previous work to house QTL for weight and length in rainbow trout, were examined for QTL in half-sibling families produced in September (1 XY male and 1 XX neomale) and December (1 XY male). In previous studies, we identified 108 candidate genes for growth expressed in the liver and white muscle in a subset of the fish used in this study. These gene sequences were BLASTN aligned against the rainbow trout and stickleback genomes to determine their location (rainbow trout) and inferred location based on synteny with the stickleback genome. Across the progeny of all three males used in the study, 63.9% of the genes with differential expression appear to co-localize with the QTL regions on 6 of the 11 chromosomes tested in these males. Genes that co-localized with QTL in the mixed-sex offspring of the two XY males primarily showed up-regulation in the muscle of large fish and were related to muscle growth, metabolism, and the stress response.
authors.affiliation: Department of Integrative Biology, 50 Stone Rd. East, University of Guelph, Guelph, ON N1G 2W1, Canada.|Department of Integrative Biology, 50 Stone Rd. East, University of Guelph, Guelph, ON N1G 2W1, Canada.
authors.forename: Andrea L
authors.initials: AL
authors.lastname: Kocmarek
authors.affiliation: Department of Integrative Biology, 50 Stone Rd. East, University of Guelph, Guelph, ON N1G 2W1, Canada.|Department of Integrative Biology, 50 Stone Rd. East, University of Guelph, Guelph, ON N1G 2W1, Canada.
authors.forename: Moira M
authors.initials: MM
authors.lastname: Ferguson
authors.affiliation: Department of Integrative Biology, 50 Stone Rd. East, University of Guelph, Guelph, ON N1G 2W1, Canada.|Department of Integrative Biology, 50 Stone Rd. East, University of Guelph, Guelph, ON N1G 2W1, Canada.
authors.forename: Roy G
authors.initials: RG
authors.lastname: Danzmann
mesh_terms: D000818:Animals; D049628:Body Size / Q000235:genetics; D002874:Chromosome Mapping; D002875:Chromosomes; D018507:Gene Expression Regulation, Developmental; D008040:Genetic Linkage; D023281:Genomics / Q000379:methods; D008297:Male; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D040641:Quantitative Trait Loci; D023701:Smegmamorpha / Q000235:genetics; D026801:Synteny / Q000235:genetics; D015854:Up-Regulation
keywords: QTL; croissance; growth; neomale; néo-mâle; rainbow trout; truite arc-en-ciel