title: T-cell receptor repertoire of human peripheral CD161hiTRAV1-2+ MAIT cells revealed by next generation sequencing and single cell analysis.
abstract: Mucosal-associated invariant T (MAIT) cells are a T-cell subset that expresses a conserved TRAV1-2 (Vα7.2) T-cell receptor (TCR) chain and the surface marker CD161. They are involved in the defence against microbes as they recognise small organic molecules of microbial origin that are presented by the non-classical MHC molecule 1 (MR1). MAIT cells express a semi-restricted TCR α chain with TRAV1-2 preferentially linked to TRAJ33, TRAJ12, or TRAJ20 which pairs with a limited set of β chains. To investigate the TCR repertoire of human CD161(hi)TRAV1-2(+) T cells in depth we analysed the α and β chains of this T-cell subset by next generation sequencing. Concomitantly we analysed 132 paired α and β chains from single cells to assess the αβ pairing preferences. We found that the CD161(hi)TRAV1-2(+) TCR repertoire in addition to the typical MAIT TCRs further contains polyclonal elements reminiscent of classical αβ T cells.
authors.affiliation: Institute of Clinical Neuroimmunology, Klinikum der Universität Muenchen, Munich, Germany. Electronic address: Kathrin.Held@med.uni-muenchen.de.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Held
authors.affiliation: Institute of Clinical Neuroimmunology, Klinikum der Universität Muenchen, Munich, Germany.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Beltrán
authors.affiliation: Department of Molecular Medicine, Max-Planck-Institute of Biochemistry, Martinsried, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Moser
authors.affiliation: Institute of Clinical Neuroimmunology, Klinikum der Universität Muenchen, Munich, Germany; Munich Cluster for Systems Neurology (SyNergy), Ludwig-Maximilian-University, Munich, Germany.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Hohlfeld
authors.affiliation: Institute of Clinical Neuroimmunology, Klinikum der Universität Muenchen, Munich, Germany; Munich Cluster for Systems Neurology (SyNergy), Ludwig-Maximilian-University, Munich, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Dornmair
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D022801:Complementarity Determining Regions / Q000737:chemistry / Q000235:genetics; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000166:cytology* / Q000276:immunology; D055651:NK Cell Lectin-Like Receptor Subfamily B / Q000235:genetics* / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry; D056510:Position-Specific Scoring Matrices; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D059010:Single-Cell Analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*
chemical_list: D022801:Complementarity Determining Regions; D055651:NK Cell Lectin-Like Receptor Subfamily B; D010446:Peptide Fragments; D016693:Receptors, Antigen, T-Cell, alpha-beta
keywords: CD161; MAIT cells; T-cell receptor; T-cell receptor repertoire