title: [Rapid identification of microorganisms by mass spectrometry in a blood culture system. Comparison of two procedures].
abstract: Rapid identification of microorganisms is critical in hospitalized infected patients. Blood culture is currently the gold standard for detecting and identifying microorganisms causing bacteremia or sepsis. The introduction of mass spectrometry by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF MS) in microbiology laboratories, especially in microorganisms growing in blood culture bottles, provides rapid identification. This study evaluates the performance of the Maldi Sepsityper Biotyper procedure (hereinafter, MS) compared to that of an in-home method (hereinafter, HF). Eight hundred and forty (840) positive blood culture bottles were processed using the HF procedure, 542 of which were also processed using MS. The organisms were identified in 670 (79.76%) and 391 (72.14%) bottles respectively (p = 0,0013). This study demonstrates the effectiveness of both procedures for identifying microorganisms directly from positive blood culture bottles. However, the HF procedure proved to be more effective than MS, especially in the presence of Gram positive organisms.
authors.affiliation: Sección Microbiología Clínica, División Laboratorio, Hospital Juan A. Fernández, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: María E
authors.initials: ME
authors.lastname: Cattani
authors.affiliation: Sección Microbiología Clínica, División Laboratorio, Hospital Juan A. Fernández, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Tamara
authors.initials: T
authors.lastname: Posse
authors.affiliation: Sección Microbiología Clínica, División Laboratorio, Hospital Juan A. Fernández, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Ricardo L
authors.initials: RL
authors.lastname: Hermes
authors.affiliation: Sección Microbiología Clínica, División Laboratorio, Hospital Juan A. Fernández, Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: sarackaufman@gmail.com.
authors.forename: Sara C
authors.initials: SC
authors.lastname: Kaufman
mesh_terms: D016470:Bacteremia / Q000382:microbiology*; D015373:Bacterial Typing Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D060085:Coinfection; D016469:Fungemia / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000097:blood / Q000382:microbiology; D006094:Gram-Positive Bacteria / Q000145:classification / Q000302:isolation & purification*; D016908:Gram-Positive Bacterial Infections / Q000097:blood / Q000382:microbiology; D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods*; D013048:Specimen Handling; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors; D015003:Yeasts / Q000145:classification / Q000302:isolation & purification*
keywords: Blood culture; Hemocultivo; Identificación; Identification; MALDITOF MS; Método rápido; Rapid methods