title: HLA-A locus allelic dropout in Sanger sequence-based typing due to the single nucleotide polymorphism of exon 1.
abstract: The DNA-based method is used widely for HLA genotyping in routine work, but some allele may be dropout in the genotyping procedure. Here, we reported a case with HLA-A allele dropout in the Sanger PCR-SBT test. The initial PCR-SBT method with a commercial agent kit was not characterized, and the result of Luminex technology indicated the dropout as a HLA-A*02 allele. Subsequently, the sequences of exons 2-4 were fully matched with the A*02:07 and A*11:01:01 by allele group-specific primer amplification PCR-SBT. On further analysis, a novel allele A*02:07:07 was identified, which has one nucleotide difference from A*02:07:01 at position 6 C>G of exon 1. According to the sequencing for 5'-UTR to 3'-UTR, the novel single nucleotide polymorphism of exon 1 was contributed to HLA-A locus allele dropout in the sample. Our results indicated multiplatform analysis is necessary when a conclusive HLA type cannot be determined by a single methodology.
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: He
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, China.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Han
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, China.
authors.forename: J
authors.initials: J
authors.lastname: He
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, China.
authors.forename: N
authors.initials: N
authors.lastname: Chen
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, China.
authors.forename: L
authors.initials: L
authors.lastname: Dong
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, China.
authors.forename: S
authors.initials: S
authors.lastname: Tao
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, China.
authors.forename: J
authors.initials: J
authors.lastname: He
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, China.
authors.forename: F
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China.|Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, China.
authors.forename: H
authors.initials: H
authors.lastname: Lv
mesh_terms: D000483:Alleles; D001483:Base Sequence; D005091:Exons; D015234:HLA-A Antigens / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D058889:Molecular Typing / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D015234:HLA-A Antigens