title: Resistance gene analogs involved in tolerant cassava--geminivirus interaction that shows a recovery phenotype.
abstract: The current literature describes recovery from virus-induced symptoms as a RNA silencing defense, but immunity-related genes, including the structurally specific resistance gene analogs (RGAs) that may play a key role in tolerance and recovery is not yet reported. In this study, the transcriptome data of tolerant cassava TME3 (which exhibits a recovery phenotype) and susceptible cassava T200 infected with South African cassava mosaic virus were explored for RGAs. Putative resistance protein analogs (RPAs) with amide-like indole-3-acetic acid-Ile-Leu-Arg (IAA-ILR) and leucine-rich repeat (LRR)-kinase conserved domains were unique to TME3. Common responsive RPAs in TME3 and T200 were the dirigent-like protein, coil-coil nucleotide-binding site (NBS) and toll-interleukin-resistance, disease resistance zinc finger chromosome condensation-like protein (DZC), and NBS-apoptosis repressor with caspase recruitment (ARC)-LRR domains. Mutations in RPAs in the MHD motif of the NBS-ARC2 subdomain associated with the recovery phase in TME3 were observed. Additionally, a cohort of 25 RGAs mined solely during the recovery process in TME3 was identified. Phylogenetic and expression analyses support that diverse RGAs are differentially expressed during tolerance and recovery. This study reveals that in cassava, a perennial crop, RGAs participate in tolerance and differentially accumulate during recovery as a complementary defense mechanism to natural occurring RNA silencing to impair viral replication.
authors.affiliation: School of Molecular and Cell Biology, University of the Witwatersrand, 1, Jan Smuts, Ave, Braamfontein, Johannesburg, 2000, South Africa. bengyellalouis@gmail.com.
authors.forename: Bengyella
authors.initials: B
authors.lastname: Louis
authors.affiliation: School of Molecular and Cell Biology, University of the Witwatersrand, 1, Jan Smuts, Ave, Braamfontein, Johannesburg, 2000, South Africa. chrissie.rey@wits.ac.za.
authors.forename: Chrissie
authors.initials: C
authors.lastname: Rey
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D017931:DNA Primers; D060467:Disease Resistance / Q000235:genetics*; D017791:Geminiviridae / Q000472:pathogenicity / Q000502:physiology*; D020869:Gene Expression Profiling; D017343:Genes, Plant; D000090263:Leucine-Rich Repeat Proteins; D002365:Manihot / Q000235:genetics* / Q000821:virology*; D058977:Molecular Sequence Annotation; D009029:Mosaic Viruses / Q000472:pathogenicity / Q000502:physiology; D009154:Mutation; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011506:Proteins; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D017931:DNA Primers; D000090263:Leucine-Rich Repeat Proteins; D010940:Plant Proteins; D011506:Proteins
keywords: Cassava; MHD motif mutations; R gene analogs; Recovery; South African cassava mosaic virus; Tolerance; Transcriptome