title: Immunoglobulin gene expression in umbilical cord blood-derived CD34⁺ hematopoietic stem/progenitor cells.
abstract: Recently, immunoglobulin (Ig) expression was reported in a variety of non-B lineage cells, including myeloid cells. We assessed whether hematopoietic stem/progenitor cells (HSC/HPCs) can express Ig. With Gene Expression Omnibus (GEO) microarray database analysis, we found that IGHM was expressed with the highest frequency and level in umbilical cord blood CD34(+) HSC/HPCs, followed by IGK@, IGHE, IGHD, IGHG1, and IGHA1, while IGL@ was nearly not expressed. Ig expression was further confirmed by molecular experiments and immunofluorescence. Moreover, HSC/HPCs-derived Ig displayed restricted/biased usages and VHDJH rearrangement patterns. These results suggest that Igs, especially IgM, may have a role in CD34(+) HSC/HPCs function.
authors.affiliation: Department of Obstetrics, Jishuitan Hospital, Beijing 100035, China.
authors.forename: Jingfang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China.
authors.forename: Miaoran
authors.initials: M
authors.lastname: Xia
authors.affiliation: Key Laboratory of Medical Immunology, Ministry of Health, Beijing 100191, China.
authors.forename: Pingzhang
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China.
authors.forename: Zihan
authors.initials: Z
authors.lastname: Geng
authors.affiliation: Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: C
authors.initials: C
authors.lastname: Cameron Yin
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China; Key Laboratory of Medical Immunology, Ministry of Health, Beijing 100191, China. Electronic address: qiuxy@bjmu.edu.cn.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qiu
mesh_terms: D018952:Antigens, CD34; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis*
chemical_list: D018952:Antigens, CD34; D007075:Immunoglobulin M
keywords: CD34(+) hematopoietic stem/progenitor cells; Immunoglobulin gene expression; V(H)DJ(H) rearrangement