title: Antiseptic mouthwashes: in vitro antibacterial activity.
abstract: Mouthwashes are used as an adjunct to tooth brushing for improving breath and preventing oral diseases. The aim of this study was to compare the in vitro Maximum Inhibitory Dilution (MID) of 3 mouthwashes with different active ingredients against mutans streptococci (MS). The products analyzed were Periogard®, Cepacol® and Plax® Fresh Mint. Their antibacterial activity was assessed in duplicate in 96-well microtiter plates against 36 clinical isolates of MS. Each mouthwash was submitted to a serial two-fold dilution (1/2.5 to 1/5120) using double concentration of Tryptose Soy Broth with 1.0% yeast extract. The final volume in each well was 100 mL plus 5 mL of a bacterial suspension, equivalent to 107 CFU/mL. They were incubated microaerobically at 37 °C for 48 hours and the MIDs determined. MID was 1/320 for Periogard® and Cepacol®, and 1/20 for PlaxR® Statistical analysis revealed that the MID of Periogard® MID did not differ from that of Cepacol® (p>0.05), and was higher than that of Plax® (p<0.05). In conclusion, the antiseptic mouthwashes containing chlorhexidine (Periogard®) and cetylpyridinium chloride (Cepacol®) had higher in vitroantibacterial activity (MID) against MS than the antiseptic mouthwash containing triclosan (Plax®), according to microbiological method employed.
authors.affiliation: Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil.
authors.forename: Evandro
authors.initials: E
authors.lastname: Watanabe
authors.affiliation: Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil.
authors.forename: Andresa P
authors.initials: AP
authors.lastname: Nascimento
authors.affiliation: Department of Restorative Dentistry, Araraquara School of Dentistry, São Paulo State University (UNESP), Araraquara, SP, Brazil.
authors.forename: Juliane M
authors.initials: JM
authors.lastname: Guerreiro-Tanomaru
authors.affiliation: Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Razaboni
authors.affiliation: Department of General and Specialized Nursing, Ribeirão Preto Nursing School, University of São Paulo (USP), Ribeirão Preto, SP, Brazil.
authors.forename: Denise
authors.initials: D
authors.lastname: de Andrade
authors.affiliation: Department of Restorative Dentistry, Araraquara School of Dentistry, São Paulo State University (UNESP), Araraquara, SP, Brazil.
authors.forename: Mário
authors.initials: M
authors.lastname: Tanomaru-Filho
mesh_terms: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D002594:Cetylpyridinium; D002710:Chlorhexidine; D009067:Mouthwashes
chemical_list: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D009067:Mouthwashes; D002594:Cetylpyridinium; D002710:Chlorhexidine