title: One-Step Identification of Five Prominent Chicken Salmonella Serovars and Biotypes.
abstract: Based on bacterial genomic data, we developed a one-step multiplex PCR assay to identify Salmonella and simultaneously differentiate the two invasive avian-adapted S. enterica serovar Gallinarum biotypes Gallinarum and Pullorum, and the most frequent, specific, and asymptomatic colonizers of chickens, serovars Enteritidis, Heidelberg, and Kentucky.
authors.affiliation: Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, Pennsylvania, USA.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, Pennsylvania, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Yue
authors.affiliation: Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, Pennsylvania, USA.
authors.forename: Shelley
authors.initials: S
authors.lastname: Rankin
authors.affiliation: Institut Pasteur, Unité des Bactéries Pathogènes Entériques, Paris, France.
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Weill
authors.affiliation: Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
authors.forename: Joachim
authors.initials: J
authors.lastname: Frey
authors.affiliation: Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, Pennsylvania, USA dmschiff@vet.upenn.edu.
authors.forename: Dieter M
authors.initials: DM
authors.lastname: Schifferli
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods; D002353:Carrier State / Q000175:diagnosis / Q000382:microbiology; D002645:Chickens / Q000382:microbiology*; D025202:Molecular Diagnostic Techniques / Q000379:methods; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000382:microbiology; D012475:Salmonella / Q000145:classification* / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000175:diagnosis* / Q000382:microbiology; D065288:Serogroup; D014730:Veterinary Medicine / Q000379:methods