title: Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.
abstract: Cell-cell interactions promote juxtacrine signals in specific subcellular domains, which are difficult to capture in the complexity of the nervous system. For example, contact between axons and Schwann cells triggers signals required for radial sorting and myelination. Failure in this interaction causes dysmyelination and axonal degeneration. Despite its importance, few molecules at the axo-glial surface are known. To identify novel molecules in axo-glial interactions, we modified the 'pseudopodia' sub-fractionation system and isolated the projections that glia extend when they receive juxtacrine signals from axons. By proteomics we identified the signalling networks present at the glial-leading edge, and novel proteins, including members of the Prohibitin family. Glial-specific deletion of Prohibitin-2 in mice impairs axo-glial interactions and myelination. We thus validate a novel method to model morphogenesis and juxtacrine signalling, provide insights into the molecular organization of the axo-glial contact, and identify a novel class of molecules in myelination.
authors.affiliation: Hunter James Kelly Research Institute, Department Biochemistry, University at Buffalo, Buffalo, New York 14203, USA.|Division of Genetics and Cell Biology, San Raffaele Hospital, Milano 20132, Italy.
authors.forename: Y
authors.initials: Y
authors.lastname: Poitelon
authors.affiliation: Division of Genetics and Cell Biology, San Raffaele Hospital, Milano 20132, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Bogni
authors.affiliation: Division of Genetics and Cell Biology, San Raffaele Hospital, Milano 20132, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Matafora
authors.affiliation: Hunter James Kelly Research Institute, Department Biochemistry, University at Buffalo, Buffalo, New York 14203, USA.
authors.forename: G
authors.initials: G
authors.lastname: Della-Flora Nunes
authors.affiliation: Hunter James Kelly Research Institute, Department Biochemistry, University at Buffalo, Buffalo, New York 14203, USA.
authors.forename: E
authors.initials: E
authors.lastname: Hurley
authors.affiliation: Hunter James Kelly Research Institute, Department Biochemistry, University at Buffalo, Buffalo, New York 14203, USA.|Division of Genetics and Cell Biology, San Raffaele Hospital, Milano 20132, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Ghidinelli
authors.affiliation: Department of Molecular and Integrative Physiology, University of Illinois and College of Medicine, Urbana Illinois 61801, USA.
authors.forename: B S
authors.initials: BS
authors.lastname: Katzenellenbogen
authors.affiliation: Division of Neuroscience, San Raffaele Hospital, Milano 20132, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Taveggia
authors.affiliation: Department of Neurology, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, New York 14203, USA.
authors.forename: N
authors.initials: N
authors.lastname: Silvestri
authors.affiliation: Division of Genetics and Cell Biology, San Raffaele Hospital, Milano 20132, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Bachi
authors.affiliation: Department of Engineering for Innovation, University of Salento, Lecce 73100, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Sannino
authors.affiliation: Hunter James Kelly Research Institute, Department Biochemistry, University at Buffalo, Buffalo, New York 14203, USA.|Division of Genetics and Cell Biology, San Raffaele Hospital, Milano 20132, Italy.|Department of Neurology, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, New York 14203, USA.
authors.forename: L
authors.initials: L
authors.lastname: Wrabetz
authors.affiliation: Hunter James Kelly Research Institute, Department Biochemistry, University at Buffalo, Buffalo, New York 14203, USA.|Division of Genetics and Cell Biology, San Raffaele Hospital, Milano 20132, Italy.|Department of Neurology, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, New York 14203, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Feltri
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism*; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000166:cytology; D007150:Immunohistochemistry; D051379:Mice; D009186:Myelin Sheath / Q000378:metabolism*; D041681:NIH 3T3 Cells; D009457:Neuroglia / Q000378:metabolism; D019899:Paracrine Communication; D000091402:Prohibitins; D040901:Proteomics; D011554:Pseudopodia / Q000378:metabolism*; D051381:Rats; D012097:Repressor Proteins / Q000378:metabolism*; D012583:Schwann Cells / Q000378:metabolism*
chemical_list: D000091402:Prohibitins; D012097:Repressor Proteins