title: Preparation of cobalt nanoparticles from polymorphic bacterial templates: A novel platform for biocatalysis.
abstract: Nanoparticles have gathered significant research attention as materials for enzyme immobilization due to their advantageous properties such as low diffusion rates, ease of manipulation, and large surface areas. Here, polymorphic cobalt nanoparticles of varied sizes and shapes were prepared using Micrococcus lylae, Bacillus subtilis, Escherichia coli, Paracoccus sp., and Haloarcula vallismortis as bacterial templates. Furthermore, nine lipases/carboxylesterases were successfully immobilized on these cobalt nanoparticles. Especially, immobilized forms of Est-Y29, LmH, and Sm23 were characterized in more detail for potential industrial applications. Immobilization of enzymes onto cobalt oxide nanoparticles prepared from polymorphic bacterial templates may have potential for efficient hydrolysis on an industrial-scale, with several advantages such as high retention of enzymatic activity, increased stability, and strong reusability.
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 140-742, Republic of Korea.
authors.forename: Eunjin
authors.initials: E
authors.lastname: Jang
authors.affiliation: School of Civil, Environmental, and Architectural Engineering, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Shim
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 140-742, Republic of Korea; Department of Molecular Cell Biology, Samsung Biomedical Research Institute, SungKyunKwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Bum Han
authors.initials: BH
authors.lastname: Ryu
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 140-742, Republic of Korea; Department of Molecular Cell Biology, Samsung Biomedical Research Institute, SungKyunKwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Deu Rae
authors.initials: DR
authors.lastname: An
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 140-742, Republic of Korea; Department of Molecular Cell Biology, Samsung Biomedical Research Institute, SungKyunKwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Wan Ki
authors.initials: WK
authors.lastname: Yoo
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, SungKyunKwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Kyeong Kyu
authors.initials: KK
authors.lastname: Kim
authors.affiliation: School of Civil, Environmental, and Architectural Engineering, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Dong-Wan
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 140-742, Republic of Korea. Electronic address: doohunkim@sookmyung.ac.kr.
authors.forename: T Doohun
authors.initials: TD
authors.lastname: Kim
mesh_terms: D000327:Adsorption; D001419:Bacteria / Q000737:chemistry*; D055162:Biocatalysis; D043182:Carboxylesterase / Q000737:chemistry; D003035:Cobalt / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D008049:Lipase / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides
chemical_list: D004800:Enzymes, Immobilized; D010087:Oxides; D003035:Cobalt; D043182:Carboxylesterase; D008049:Lipase; C060728:cobalt oxide
keywords: Biocatalysts; Cobalt oxide nanoparticles; Enzyme immobilization