title: Rab GTPases and kinesin motors in endosomal trafficking.
abstract: The endocytic pathway is composed of distinct types of endosomes that vary in shape, function, and molecular composition. In addition, endosomes are highly dynamic structures that continuously receive, sort, and deliver molecules to other organelles. Among organizing machineries that contribute to endosomal functions, Rab GTPases and kinesin motors play critical roles. Rab proteins define the identity of endosomal subdomains by recruiting set of effectors among which kinesins shape and transport membranous carriers along the microtubule network. In this review, we provide detailed protocols from live cell imaging to electron microscopy and biochemical approaches to address how Rab and kinesin proteins cooperate molecularly and functionally within the endocytic pathway.
authors.affiliation: Institut Curie, PSL Research University, Paris, France; CNRS UMR 144, Structure and Membrane Compartments, Paris, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Delevoye
authors.affiliation: Institut Curie, PSL Research University, Paris, France; CNRS UMR 144, Molecular Mechanisms of Intracellular Transport, Paris, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Goud
mesh_terms: D004705:Endocytosis; D011992:Endosomes / Q000201:enzymology*; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D055503:Protein Multimerization; D021381:Protein Transport; D012441:Saccharomyces cerevisiae; D020691:rab GTP-Binding Proteins / Q000502:physiology*
chemical_list: C417670:KIF13A protein, human; D016547:Kinesins; D020691:rab GTP-Binding Proteins
keywords: Degradation and recycling assay; Electron microscopy; Epidermal growth factor; Kinesin; Live cell imaging; Multivesicular endosome; Rab GTPase; Recycling endosome; Sorting endosome; Transferrin; Yeast two-hybrid