title: Assessment of the eye irritation potential of chemicals: A comparison study between two test methods based on human 3D hemi-cornea models.
abstract: We have recently developed two hemi-cornea models (Bartok et al., Toxicol in Vitro 29, 72, 2015; Zorn-Kruppa et al. PLoS One 9, e114181, 2014), which allow the correct prediction of eye irritation potential of chemicals according to the United Nations globally harmonized system of classification and labeling of chemicals (UN GHS). Both models comprise a multilayered epithelium and a stroma with embedded keratocytes in a collagenous matrix. These two models were compared, using a set of fourteen test chemicals. Their effects after 10 and 60 minutes (min) exposure were assessed from the quantification of cell viability using the MTT reduction assay. The first approach separately quantifies the damage inflicted to the epithelium and the stroma. The second approach quantifies the depth of injury by recording cell death as a function of depth. The classification obtained by the two models was compared to the Draize rabbit eye test and an ex vivo model using rabbit cornea (Jester et al. Toxicol in Vitro. 24, 597-604, 2010). With a 60 min exposure, both of our models are able to clearly differentiate UN GHS Category 1 and UN GHS Category 2 test chemicals.
authors.affiliation: Jacobs University Bremen, Department of Life Sciences and Chemistry, Campus Ring 1, 28759 Bremen, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Tandon
authors.affiliation: Jacobs University Bremen, Department of Life Sciences and Chemistry, Campus Ring 1, 28759 Bremen, Germany. Electronic address: dr.melinda.bartok@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Bartok
authors.affiliation: University Medical Center Hamburg-Eppendorf, Department of Dermatology and Venerology, Martinistraße 52, 20246 Hamburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Zorn-Kruppa
authors.affiliation: University Medical Center Hamburg-Eppendorf, Department of Dermatology and Venerology, Martinistraße 52, 20246 Hamburg, Germany.
authors.forename: J M
authors.initials: JM
authors.lastname: Brandner
authors.affiliation: Jacobs University Bremen, Department of Life Sciences and Chemistry, Campus Ring 1, 28759 Bremen, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Gabel
authors.affiliation: Jacobs University Bremen, Department of Life Sciences and Chemistry, Campus Ring 1, 28759 Bremen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Engelke
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003315:Cornea / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D007509:Irritants / Q000633:toxicity*; D008954:Models, Biological; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D007509:Irritants
keywords: Globally harmonized system of classification and labeling of chemicals; Human 3D hemi-cornea model; Relative MTT-depth of injury; The Draize eye irritation test