title: Antioxidant Effect of Sepia Ink Extract on Extrahepatic Cholestasis Induced by Bile Duct Ligation in Rats.
abstract: OBJECTIVE
  The aim of our study was to assess the complications of hepatic fibrosis associated with bile duct ligation and the potential curative role of sepia ink extract in hepatic damage induced by bile duct ligation.
  
  
  METHODS
  Rattus norvegicus rats were divided into 3 groups: Sham-operated group, model rats that underwent common bile duct ligation (BDL), and BDL rats treated orally with sepia ink extract (200 mg/kg body weight) for 7, 14, and 28 d after BDL.
  
  
  RESULTS
  There was a significant reduction in hepatic enzymes, ALP, GGT, bilirubin levels, and oxidative stress in the BDL group after treatment with sepia ink extract. Collagen deposition reduced after sepia ink extract treatment as compared to BDL groups, suggesting that the liver was repaired. Histopathological examination of liver treated with sepia ink extract showed moderate degeneration in the hepatic architecture and mild degeneration in hepatocytes as compared to BDL groups.
  
  
  CONCLUSIONS
  Sepia ink extract provides a curative effect and an antioxidant capacity on BDL rats and could ameliorate the complications of liver cholestasis.
authors.affiliation: Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
authors.forename: Hanan
authors.initials: H
authors.lastname: Saleh
authors.affiliation: Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
authors.forename: Amel M
authors.initials: AM
authors.lastname: Soliman
authors.affiliation: Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
authors.forename: Ayman S
authors.initials: AS
authors.lastname: Mohamed
authors.affiliation: Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
authors.forename: Mohamed-Assem S
authors.initials: MA
authors.lastname: Marie
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001652:Bile Ducts / Q000601:surgery*; D015415:Biomarkers / Q000097:blood; D001651:Cholestasis, Extrahepatic / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D003094:Collagen / Q000378:metabolism; D007281:Ink; D008099:Liver / Q000378:metabolism; D008111:Liver Function Tests; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D049833:Sepia / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D003094:Collagen
keywords: Bile duct ligation; Hepatic fibrosis; Histopathological examination; Liver collagen percentage; Oxidative stress