title: Development of a novel ultrasensitive enzyme immunoassay for human glutamic acid decarboxylase 65 antibody.
abstract: BACKGROUND
  We developed a novel, ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for determination of glutamic acid decarboxylase autoantibody concentrations in serum samples from patients with type 2 diabetes.
  
  
  METHODS
  We developed an immune complex transfer enzyme immunoassay for glutamic acid decarboxylase autoantibody and measured glutamic acid decarboxylase autoantibody from 22 patients with type 1 diabetes, 29 patients with type 2 diabetes, and 32 healthy controls.
  
  
  RESULTS
  A conventional ELISA kit identified 10 patients with type 1 diabetes and one patient with type 2 diabetes as glutamic acid decarboxylase autoantibody positive, whereas 15 patients with type 1 diabetes and six patients with type 2 diabetes were identified as glutamic acid decarboxylase autoantibody positive using immune complex transfer enzyme immunoassay.
  
  
  CONCLUSIONS
  Immune complex transfer enzyme immunoassay is a highly sensitive and specific assay for glutamic acid decarboxylase autoantibody and might be clinically useful for diabetic onset prediction and early diagnosis.
authors.affiliation: Department of Nutrition, University of Kochi, Kochi, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Numata
authors.affiliation: Clinical Research Science, Department of Medicine, Teikyo University Chiba Medical Center, Chiba, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Katakami
authors.affiliation: National Hospital Organization Miyazaki Higashi Hospital, Miyazaki, Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Inoue
authors.affiliation: Division of Pediatrics, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
authors.forename: Hirotake
authors.initials: H
authors.lastname: Sawada
authors.affiliation: Institute for Health Sciences, Tokushima Bunri University, Tokushima, Japan shashida@tokushima.bunri-u.ac.jp.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Hashida
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005968:Glutamate Decarboxylase / Q000276:immunology*; D006801:Humans; D012680:Sensitivity and Specificity
chemical_list: D001323:Autoantibodies; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2
keywords: Enzyme immunoassay; diabetes; glutamic acid decarboxylase 65; glutamic acid decarboxylase autoantibody; slowly progressive insulin-dependent diabetes mellitus