title: A Novel Function of TET2 in CNS: Sustaining Neuronal Survival.
abstract: DNA dioxygenases Ten-Eleven Translocation (TET) proteins can catalyze the conversion of 5-methylcytosine (5mC) of DNA to 5-hydroxymethylcytosine (5hmC), and thereby alter the epigenetic state of DNA. The TET family includes TET1, TET2 and TET3 members in mammals. Recently, accumulative research uncovered that TET1-3 occur abundantly in the central nervous system (CNS), and their biological functions have just begun to be investigated. In the present study, we demonstrated that mRNA and protein of TET2 were highly expressed in the cerebral cortex and hippocampus along the whole brain-development process. Further studies showed that TET2 was expressed in various types of cells, especially in most neurons. Subcellular distribution pattern implicated that TET2 is localized in both nucleus and cytoplasm of neurons. Down-regulation of TET2 in cultured cortical neurons with RNA interference implied that TET2 was required for cell survival. In all, our results indicate that neuronal TET2 is positively involved in the regulation of cell survival.
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Anesthesiology, School of Stomatology, the Fourth Military Medical University, Xi'an 710032, China. miyajing@163.com.|Institute of Basic Medicine Science, Xi'an Medical University, Xi'an 710021, China. miyajing@163.com.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Mi
authors.affiliation: Institute of Basic Medicine Science, Xi'an Medical University, Xi'an 710021, China. gxc199281003@163.com.|School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. gxc199281003@163.com.
authors.forename: Xingchun
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Cell and Developmental Biology, University of Colorado Denver, Denver, CO 80045, USA. jin.dai@ucdenver.edu.
authors.forename: Jinxiang
authors.initials: J
authors.lastname: Dai
authors.affiliation: Institute of Nano Biomedicine and Engineering, Department of Instrument Science and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of Ministry of Education, School of Electronic Information and Electronic Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. mayuels@126.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Anesthesiology, School of Stomatology, the Fourth Military Medical University, Xi'an 710032, China. xlx116@fmmu.edu.cn.
authors.forename: Lixian
authors.initials: L
authors.lastname: Xu
authors.affiliation: School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. weilinjin@sjtu.edu.cn.|Institute of Nano Biomedicine and Engineering, Department of Instrument Science and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of Ministry of Education, School of Electronic Information and Electronic Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. weilinjin@sjtu.edu.cn.
authors.forename: Weilin
authors.initials: W
authors.lastname: Jin
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000235:genetics; D002490:Central Nervous System / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D049308:Dioxygenases; D015870:Gene Expression; D020869:Gene Expression Profiling; D051379:Mice; D009474:Neurons / Q000378:metabolism; D021381:Protein Transport; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D049308:Dioxygenases; C551914:Tet2 protein, mouse
keywords: CNS; RNA interference; TET2; cell survival; neurons