title: Transcriptomic profiling revealed the regulatory mechanism of Arabidopsis seedlings response to oxidative stress from cryopreservation.
abstract: Elevated antioxidant status and positive abiotic stress response in dehydration enhance cell resistance to cryoinjury, and controlling oxidative damage via reactive oxygen species homeostasis maintenance leads to high survival. Cryoprotectants are important for cell survival in cryopreservation, but high concentrations can also cause oxidative stress. Adding vitamin C to the cryoprotectant doubled the survival ratio in Arabidopsis thaliana 60-h seedlings (seedlings after 60-h germination) cryopreservation. In this study, the metabolites and transcriptional profiling of 60-h seedlings were analyzed in both the control cryopreservation procedure (CCP) and an improved cryopreservation procedure (ICP) to reveal the mechanism of plant cell response to oxidative stress from cryopreservation. Reactive oxygen species (ROS) and peroxidation levels reached a peak after rapid cooling-warming in CCP, which were higher than that in ICP. In addition, gene regulation was significantly increased in CCP and decreased in ICP during rapid cooling-warming. Before cryogenic treatment, the number of specifically regulated genes was nearly 10 times higher in ICP dehydration than CCP dehydration. Among these genes, DREBs/CBFs were beneficial to cope with cryoinjury, and calcium-binding protein, OXI1, WRKY and MYB family members as key factors in ROS signal transduction activated the ROS-producing and ROS-scavenging networks including AsA-GSH and GPX cycles involved in scavenging H2O2. Finally, elevated antioxidant status and oxidative stress response in the improved dehydration enhanced seedling resistance to cryogenic treatment, maintained ROS homeostasis and improved cell recovery after cryopreservation.
authors.affiliation: Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800, Rd. Dong Chuan, Shanghai, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.affiliation: Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800, Rd. Dong Chuan, Shanghai, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800, Rd. Dong Chuan, Shanghai, People's Republic of China.
authors.forename: Guan-qun
authors.initials: GQ
authors.lastname: Chen
authors.affiliation: United States Department of Agriculture-Agricultural Research Service, National Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR, 97333-2521, USA.
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Reed
authors.affiliation: Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800, Rd. Dong Chuan, Shanghai, People's Republic of China. shenxh62@sjtu.edu.cn.
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Shen
authors.affiliation: Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800, Rd. Dong Chuan, Shanghai, People's Republic of China. chhy@sjtu.edu.cn.
authors.forename: Huo-ying
authors.initials: HY
authors.lastname: Chen
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D001205:Ascorbic Acid / Q000378:metabolism; D015925:Cryopreservation; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D006861:Hydrogen Peroxide / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism*; D036226:Seedlings / Q000235:genetics* / Q000502:physiology; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid
keywords: Arabidopsis thaliana; Cryopreservation; Oxidative stress; Reactive oxygen species; Stress physiology; Transcriptomics