title: Micro-anatomical and functional assessment of ciliated epithelium in mouse trachea using optical coherence phase microscopy.
abstract: Motile cilia perform a range of important mechanosensory and chemosensory functions, along with expulsion of mucus and inhaled pathogens from the lungs. Here we demonstrate that spectral domain optical coherence phase microscopy (SD-OCPM), which combines the principles of optical coherence tomography (OCT) and confocal microscopy, is particularly well-suited for characterization of both morphology and the ciliary dynamics of mouse trachea. We present micro-anatomical images of mouse trachea, where different cell types can be clearly visualized. The phase contrast, which measures the sub-nanometer changes in axial optical pathlength is used to determine the frequency and direction of cilia beatings.
authors.forename: Rehman
authors.initials: R
authors.lastname: Ansari
authors.forename: Christian
authors.initials: C
authors.lastname: Buj
authors.forename: Mario
authors.initials: M
authors.lastname: Pieper
authors.forename: Peter
authors.initials: P
authors.lastname: König
authors.forename: Achim
authors.initials: A
authors.lastname: Schweikard
authors.forename: Gereon
authors.initials: G
authors.lastname: Hüttmann