title: Gel-free/label-free proteomic analysis of root tip of soybean over time under flooding and drought stresses.
abstract: Growth in the early stage of soybean is markedly inhibited under flooding and drought stresses. To explore the responsive mechanisms of soybean, temporal protein profiles of root tip under flooding and drought stresses were analyzed using gel-free/label-free proteomic technique. Root tip was analyzed because it was the most sensitive organ against flooding, and it was beneficial to root penetration under drought. UDP glucose: glycoprotein glucosyltransferase was decreased and increased in soybean root under flooding and drought, respectively. Temporal protein profiles indicated that fermentation and protein synthesis/degradation were essential in root tip under flooding and drought, respectively. In silico protein-protein interaction analysis revealed that the inductive and suppressive interactions between S-adenosylmethionine synthetase family protein and B-S glucosidase 44 under flooding and drought, respectively, which are related to carbohydrate metabolism. Furthermore, biotin/lipoyl attachment domain containing protein and Class II aminoacyl tRNA/biotin synthetases superfamily protein were repressed in the root tip during time-course stresses. These results suggest that biotin and biotinylation might be involved in energy management to cope with flooding and drought in early stage of soybean-root tip.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan; National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.
authors.forename: MyeongWon
authors.initials: M
authors.lastname: Oh
authors.affiliation: Maebashi Institute of Technology, Maebashi 371-0816, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Sakata
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan; National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan. Electronic address: skomatsu@affrc.go.jp.
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu
mesh_terms: D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D002241:Carbohydrates / Q000737:chemistry; D016000:Cluster Analysis; D018556:Crops, Agricultural / Q000378:metabolism; D055864:Droughts; D005285:Fermentation; D055868:Floods; D018506:Gene Expression Regulation, Plant; D013058:Mass Spectrometry; D008716:Methionine Adenosyltransferase / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D014176:Protein Biosynthesis; D017510:Protein Folding; D025941:Protein Interaction Mapping; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013025:Glycine max / Q000254:growth & development / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D002241:Carbohydrates; D010940:Plant Proteins; D020543:Proteome; D001710:Biotin; D008716:Methionine Adenosyltransferase
keywords: Drought; Flooding; Proteomics; Root tip; Soybean; Temporal protein profiles