title: Protective role of extracts of grape skin and grape flesh on ethanol-induced oxidative stress, inflammation and histological alterations in rat brain.
abstract: Ethanol consumption has deleterious effects on all organs and especially on brain. In our study, the radical scavenging properties of grape skin and grape flesh (contains natural antioxidants like polyphenols) was determined in vitro and for in vivo studies, male Wistar rats (16-18 week-old) and 100-120 gm weight were divided in five groups of six animals each. One group were fed with ethanol (1.6 gm/kg body weight), while second group were fed with ethanol (1.6 g/kg body weight) and grape skin extract (2.5 g/kg body weight), the third group were fed with ethanol (1.6 g/kg body weight) and grape flesh extract, daily once for 12 weeks, while the fourth group were fed with ethanol (1.6 g/kg body weight) and tocopheryl acetate (80 mg/kg/day) daily once for 12 weeks. Results of in vitro studies indicate that grape skin extracts showed significant radical scavenging properties (ROS). There was also significantly alteration of serum cytokines in our study.
authors.affiliation: a Department of Biochemistry , NRI Institute of Medical Sciences , Vizag , India .
authors.forename: Sukhes
authors.initials: S
authors.lastname: Mukherjee
authors.affiliation: b Department of Biochemistry , College of Medicine & JNM Hospital , Kalyani , India , and.
authors.forename: Subir Kumar
authors.initials: SK
authors.lastname: Das
authors.affiliation: c Department of Biochemistry , Amrita School of Medicine , Kochi , Kerala , India.
authors.forename: D M
authors.initials: DM
authors.lastname: Vasudevan
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D000431:Ethanol / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D007249:Inflammation / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D019441:Plant Epidermis / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D027843:Vitis / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D010936:Plant Extracts; D000431:Ethanol
keywords: Ethanol; ROS; grape; oxidative stress; polyphenols