title: [Agroindustrial wastes methanization and bacterial composition in anaerobic digestion].
abstract: The tons of organic waste that are annually generated by agro-industry, can be used as raw material for methane production. For this reason, it is important to previously perform biodegradability tests to organic wastes for their full scale methanization. This paper addresses biodegradability, methane production and the behavior of populations of eubacteria and archaeabacteria during anaerobic digestion of banana, mango and papaya agroindustrial wastes. Mango and banana wastes had higher organic matter content than papaya in terms of their volatile solids and total solid rate (94 and 75% respectively). After 63 days of treatment, the highest methane production was observed in banana waste anaerobic digestion: 63.89ml CH4/per gram of chemical oxygen demand of the waste. In the PCR-DGGE molecular analysis, different genomic footprints with oligonucleotides for eubacteria and archeobacteria were found. Biochemical methane potential results proved that banana wastes have the best potential to be used as raw material for methane production. The result of a PCR- DGGE analysis using specific oligonucleotides enabled to identify the behavior of populations of eubacteria and archaeabacteria present during the anaerobic digestion of agroindustrial wastes throughout the process.
authors.affiliation: Ingeniería en Tecnología Ambiental, Universidad Politécnica de Chiapas, Suchiapa, Chiapas, México.
authors.forename: María E
authors.initials: ME
authors.lastname: González-Sánchez
authors.affiliation: Ingeniería en Tecnología Ambiental, Universidad Politécnica de Chiapas, Suchiapa, Chiapas, México.
authors.forename: Sergio
authors.initials: S
authors.lastname: Pérez-Fabiel
authors.affiliation: División Agroalimentaria, Universidad Tecnológica de la Selva, Ocosingo, Chiapas, México.
authors.forename: Arnoldo
authors.initials: A
authors.lastname: Wong-Villarreal
authors.affiliation: El Colegio de la Frontera Sur (ECOSUR), Tapachula, Chiapas, México.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Bello-Mendoza
authors.affiliation: Ingeniería en Tecnología Ambiental, Universidad Politécnica de Chiapas, Suchiapa, Chiapas, México. Electronic address: gyanez@upchiapas.edu.mx.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Yañez-Ocampo
mesh_terms: D000383:Agriculture; D000693:Anaerobiosis; D001105:Archaea / Q000302:isolation & purification* / Q000378:metabolism; D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism; D001673:Biodegradation, Environmental; D056804:Biofuels; D019149:Bioreactors; D029441:Carica; D004269:DNA, Bacterial / Q000032:analysis; D007220:Industrial Waste; D031022:Mangifera; D008697:Methane / Q000096:biosynthesis* / Q000302:isolation & purification; D010943:Plantago; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012037:Refuse Disposal; D021521:Ribotyping; D062611:Solid Waste
chemical_list: D056804:Biofuels; D004269:DNA, Bacterial; D007220:Industrial Waste; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D062611:Solid Waste; D008697:Methane
keywords: Agroindustrial wastes; Anaerobic digestion; Bacterias metanogénicas; Biochemical Methane Potential; Digestión anaerobia; Metanogenic bacteria; Potencial bioquímico de metano; Residuos agroindustriales