title: Quantitation of Oxidative Stress Gene Expression in Human Cell Lines Treated with Water-Dispersible MnO Nanoparticles.
abstract: The objective of this study was to assess cytotoxicity of engineered MnO nanoparticles by quantifying the reactive oxygen species (ROS) related genes (glutathione S-transferase (GST) and catalase) using real time-polymerase chain reaction (RT-PCR) and molecular beacon (MB) technologies. Monodisperse MnO nanoparticles of 14 nm in size were synthesized by the encapsulation of polyethyleneglycol (PEG)-phospholipid shell around the MnO core to endow high water-dispersibility and biocompatibility. In vitro cytotoxicity was evaluated at different concentrations (10, 50 and 100 μg/ml) and incubation times (12, 24 and 48 h) with human cancer cell lines (glioblastoma, lung adenocarcinoma and neuroblastoma cells). Both genetic and cellular cytotoxic screening methods produced consistent results, showing that GST and catalase ROS gene expression was maximized in 24 h incubation at 100 μg/ml concentration of MnO nanoparticles for each cell line. However, the cytotoxicity effect of the PEG-phospholipid coated MnO nanoparticle was not significant compared with control experiments, demonstrating its high potential in the applications of nanomedicines for a diagnostic and therapeutic tool.
authors.forename: Jong Seob
authors.initials: JS
authors.lastname: Choi
authors.forename: Jong Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Hyon Bin
authors.initials: HB
authors.lastname: Na
authors.forename: Tae Seok
authors.initials: TS
authors.lastname: Seo
mesh_terms: D002374:Catalase / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D015870:Gene Expression; D005982:Glutathione Transferase / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D017895:Manganese Compounds / Q000737:chemistry*; D057054:Molecular Imaging; D053758:Nanoparticles / Q000737:chemistry*; D018384:Oxidative Stress / Q000235:genetics*; D010087:Oxides / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017895:Manganese Compounds; D010087:Oxides; C027424:manganese oxide; D002374:Catalase; D005982:Glutathione Transferase