title: Nitric oxide and oxidative stress in placental explant cultures.
abstract: Placental explant culture, and cellular cytolysis and cellular differentiation have been previously studied. However, oxidative stress and nitric oxide profiles have not been evaluated in these systems. The aim of this study was to determine the release of lipid peroxidation and nitric oxide from placental explants cultured over a seven day period. Placental explants were maintained for seven days in culture and the medium was changed every 24 hours. The response was assessed in terms of syncytiotrophoblast differentiation (human chorionic gonadotropin, hCG), cellular cytolysis (lactate dehydrogenase, LDH), oxidative stress (thiobarbituric acid reactive substances, TBARS), and nitric oxide (NO). Levels of hCG increased progressively from day two to attain its highest level on days four and five after which it decreased gradually. In contrast, the levels of LDH, TBARS, and NO were elevated in the early days of placental culture when new syncytiotrophoblast from cytotrophoblast were forming and also in the last days of culture when tissue was declining. In conclusion, the levels of NO and lipid peroxidation follow a pattern similar to LDH and contrary to hCG. Future placental explant studies to evaluate oxidative stress and NO should consider the physiological changes inherent during the time of culture.
authors.affiliation: a Facultad de Medicina , Universidad Central de Venezuela , Caracas , Venezuela and.|b Departamento de Biología de Organismos , Universidad Simón Bolívar , Baruta , Venezuela.
authors.forename: Juvic M
authors.initials: JM
authors.lastname: Goncalves
authors.affiliation: a Facultad de Medicina , Universidad Central de Venezuela , Caracas , Venezuela and.
authors.forename: Ysabel C
authors.initials: YC
authors.lastname: Casart
authors.affiliation: b Departamento de Biología de Organismos , Universidad Simón Bolívar , Baruta , Venezuela.
authors.forename: María I
authors.initials: MI
authors.lastname: Camejo
mesh_terms: D000293:Adolescent; D000328:Adult; D006063:Chorionic Gonadotropin / Q000378:metabolism; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation; D009569:Nitric Oxide / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D009924:Organ Culture Techniques; D018384:Oxidative Stress; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014327:Trophoblasts / Q000502:physiology; D055815:Young Adult
chemical_list: D006063:Chorionic Gonadotropin; D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; D007770:L-Lactate Dehydrogenase; D052250:Nitric Oxide Synthase Type III
keywords: hCG; lactate dehydrogenase; lipid peroxidation; nitric oxide; oxidative stress; placental explants