title: [Agrobacterium-mediated transformation of Cymbidium sinensis].
abstract: Genetic transformation is an effective method to improve breeding objective traits of orchids. However, there is little information about genetic transformation of Cymbidium sinensis. Rhizomes from shoot-tip culture of C. sinensis cv. 'Qijianbaimo' were used to establish a practical transformation protocol of C. sinensis. Pre-culture time, concentration and treating methods of acetosyringone, concentration of infection bacteria fluid (OD600), infection time, and co-culture time had significant effects on β-glucuronidase (GUS) transient expression rate of C. sinensis cv. 'Qijianbaimo' rhizome. The GUS transient expression rate of rhizome was the highest (11.67%) when rhizomes pre-cultured for 39 d were soaked in bacterium suspension (OD600 = 0.9) supplemented with 200 μmol/L acetosyringone for 35 min, followed by culturing on co-culture medium supplemented with 200 μmol/L acetosyringone for 7 d. Under this transformation conditions, 3 transgenic plantlets, confirmed by GUS histochemical assay and PCR, were obtained from 400 regenerated plantlets, and the genetic transformation rate was 0.75%. This proved that it was feasible to create new cultivars by the use of Agrobacterium-mediated genetic transformation in C. sinense.
authors.forename: Li
authors.initials: L
authors.lastname: Xie
authors.forename: Fen
authors.initials: F
authors.lastname: Wang
authors.forename: Ruizhen
authors.initials: R
authors.lastname: Zeng
authors.forename: Herong
authors.initials: H
authors.lastname: Guo
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D060054:Agrobacterium; D018920:Coculture Techniques; D005818:Genetic Engineering; D005966:Glucuronidase; D029595:Orchidaceae / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic
chemical_list: D005966:Glucuronidase