title: Principles of Whole-Genome Amplification.
abstract: Modern molecular biology relies on large amounts of high-quality genomic DNA. However, in a number of clinical or biological applications this requirement cannot be met, as starting material is either limited (e.g., preimplantation genetic diagnosis (PGD) or analysis of minimal residual cancer) or of insufficient quality (e.g., formalin-fixed paraffin-embedded tissue samples or forensics). As a consequence, in order to obtain sufficient amounts of material to analyze these demanding samples by state-of-the-art modern molecular assays, genomic DNA has to be amplified. This chapter summarizes available technologies for whole-genome amplification (WGA), bridging the last 25 years from the first developments to currently applied methods. We will especially elaborate on research application, as well as inherent advantages and limitations of various WGA technologies.
authors.affiliation: Project Group, Personalized Tumor Therapy, Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Josef-Engert-Straße 9, 93053, Regensburg, Germany.
authors.forename: Zbigniew Tadeusz
authors.initials: ZT
authors.lastname: Czyz
authors.affiliation: Project Group, Personalized Tumor Therapy, Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Josef-Engert-Straße 9, 93053, Regensburg, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kirsch
authors.affiliation: Project Group, Personalized Tumor Therapy, Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Josef-Engert-Straße 9, 93053, Regensburg, Germany. bernhard.michael.polzer@item.fraunhofer.de.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Polzer
mesh_terms: D000818:Animals; D016678:Genome; D023281:Genomics / Q000379:methods*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques
keywords: Ligation-mediated amplification; Multiple displacement amplification; PCR-based amplification; Whole-genome amplification