title: MicroRNA detection based on analyte triggered nanoparticle localization on a tetrahedral DNA modified electrode followed by hybridization chain reaction dual amplification.
abstract: We have constructed an ultrasensitive method for microRNA detection based on analyte triggered nanoparticle localization and hybridization chain reaction dual amplification. The limit of detection is 2 aM. Furthermore, the applicability of this method for microRNA assay in real samples is demonstrated by the analysis of cell lysates.
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, P. R. China. miaopeng@sibet.ac.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Miao
authors.forename: Yuguo
authors.initials: Y
authors.lastname: Tang
authors.forename: Jian
authors.initials: J
authors.lastname: Yin
mesh_terms: D015374:Biosensing Techniques; D002460:Cell Line; D004247:DNA / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D012834:Silver / Q000737:chemistry
chemical_list: D035683:MicroRNAs; D012834:Silver; D006046:Gold; D004247:DNA