title: Elucidation of innovative antibiofilm materials.
abstract: It is known for roughly a decade that bacterial communities (called biofilms) are responsible for significant enhanced antibiotherapy resistance. Biofilms are involved in tissue persistent infection, causing direct or collateral damage leading to chronic wounds development and impairing natural wound healing. In this study, we are interested in the development of supported protein materials which consist of asymmetric membranes as reservoir supports for the incorporation and controlled release of biomolecules capable of dissolving biofilms (or preventing their formation) and their use as wound dressing for chronic wound treatment. In a first step, polyhydroxyalkanoates (PHAs) asymmetric membranes were prepared using wet phase inversion technique. Scanning microscopy (SEM) analysis has showed the influence of different processing parameters. In a second step, the porous side of the membranes were functionalized with a surface treatment and then loaded with the antibiofilm agent (dispersin B). In a third step, the properties and antibiofilm performance of the loaded-membranes were evaluated. Exposure of Staphylococcus epidermidis biofilms to such systems weakly inhibited biofilm formation (weak preventive effect) but caused their detachment and disaggregation (strong curative effect). These initial results are promising since they open the way to a new generation of effective tools in the struggle against persistent bacterial infections exhibiting enhanced antibiotherapy resistance, and in particular in the case of infected chronic wounds.
authors.affiliation: Normandy University, France; Université de Rouen, Laboratoire Polymères, Biopolymères et Surfaces, Bd. Maurice de Broglie, F-76821 Mont Saint Aignan Cedex, France; CNRS, UMR 6270 & FR 3038, F-76821 Mont Saint Aignan Cedex, France.
authors.forename: Aracelys
authors.initials: A
authors.lastname: Marcano
authors.affiliation: Normandy University, France; Université de Rouen, Laboratoire Polymères, Biopolymères et Surfaces, Bd. Maurice de Broglie, F-76821 Mont Saint Aignan Cedex, France; CNRS, UMR 6270 & FR 3038, F-76821 Mont Saint Aignan Cedex, France.
authors.forename: Ousmane
authors.initials: O
authors.lastname: Ba
authors.affiliation: Normandy University, France; Université de Rouen, Laboratoire Polymères, Biopolymères et Surfaces, Bd. Maurice de Broglie, F-76821 Mont Saint Aignan Cedex, France; CNRS, UMR 6270 & FR 3038, F-76821 Mont Saint Aignan Cedex, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Thebault
authors.affiliation: Normandy University, France; Université de Rouen, Laboratoire Polymères, Biopolymères et Surfaces, Bd. Maurice de Broglie, F-76821 Mont Saint Aignan Cedex, France; CNRS, UMR 6270 & FR 3038, F-76821 Mont Saint Aignan Cedex, France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Crétois
authors.affiliation: Normandy University, France; Université de Rouen, Laboratoire Polymères, Biopolymères et Surfaces, Bd. Maurice de Broglie, F-76821 Mont Saint Aignan Cedex, France; CNRS, UMR 6270 & FR 3038, F-76821 Mont Saint Aignan Cedex, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Marais
authors.affiliation: Normandy University, France; Université de Rouen, Laboratoire Polymères, Biopolymères et Surfaces, Bd. Maurice de Broglie, F-76821 Mont Saint Aignan Cedex, France; CNRS, UMR 6270 & FR 3038, F-76821 Mont Saint Aignan Cedex, France. Electronic address: anthony.duncan@univ-rouen.fr.
authors.forename: Anthony C
authors.initials: AC
authors.lastname: Duncan
mesh_terms: D001672:Biocompatible Materials; D018441:Biofilms; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial
keywords: Antibiofilm PHA-based membranes; Biofilm detachment; Biomaterials; Chronic wound dressing; Dispersin B; Drug delivery; Wet phase inversion