title: Analyzing the functions of Rab11-effector proteins during cell division.
abstract: Recycling endosomes recently have emerged as major regulators of cytokinesis and abscission steps of cell division. Rab11-endosomes in particular were shown to transport proteins to the mitotic ingression furrow and play a key role in establishing the abscission site. Rab11 GTPase functions by binding and activating various effector proteins, such as Rab11 family interacting proteins (FIPs). FIPs appear to be at the core of many Rab11 functions, with FIP3 playing a role in targeting of the Rab11-endosomes during mitosis. Here we summarize the newest finding regarding the roles and regulation of FIP3 and Rab11 complex, as well as describe the methods developed to analyze membrane and cytoskeleton dynamics during abscission step of cytokinesis.
authors.affiliation: Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA. Electronic address: rytis.prekeris@ucdenver.edu.
authors.forename: Rytis
authors.initials: R
authors.lastname: Prekeris
mesh_terms: D000818:Animals; D048749:Cytokinesis; D004285:Dogs; D011992:Endosomes / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism*; D061985:Madin Darby Canine Kidney Cells; D008856:Microscopy, Fluorescence; D021381:Protein Transport; D059008:Time-Lapse Imaging; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D051550:I-kappa B Kinase; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C491731:IKBKG protein, human
keywords: Abscission; Cytokinesis; FIP3; Midbody; Mitosis; Rab11; Recycling endosomes