title: Umbilical cord mesenchymal stem cell-conditioned media prevent muscle atrophy by suppressing muscle atrophy-related proteins and ROS generation.
abstract: The therapeutic potential of mesenchymal stem cell-conditioned medium (MSC-CM) has been reported with various types of disease models. Here, we examine the therapeutic effect of umbilical cord MSC-CM (UCMSC-CM) on muscle-related disease, using a dexamethasone (Dex)-induced muscle atrophy in vitro model. The expressions of muscle atrophy-related proteins (MuRF-1 and MAFbx) and muscle-specific proteins (desmin and myogenin) were evaluated by Western blot analysis. The level of production of reactive oxygen species (ROS) was determined using a 2',7'-dichlorofluorescein diacetate (DCFDA) dye assay. The expression of antioxidant enzymes (copper/zinc-superoxide dismutase (Cu/Zn-SOD), manganese superoxide dismutase (MnSOD), glutathione peroxidase-1 (GPx-1), and catalase (CAT)) was verified by reverse transcription polymerase chain reaction (RT-PCR). When L6 cells were exposed to Dex, the expression of muscle atrophy-related proteins was increased by 50-70%, and the expression of muscle-specific proteins was in turn decreased by 23-40%. Conversely, when the L6 cells were co-treated with UCMSC-CM and Dex, the expression of muscle atrophy-related proteins was reduced in a UCMSC-CM dose-dependent manner and the expression of muscle-specific proteins was restored to near-normal levels. Moreover, ROS generation was effectively suppressed and the expression of antioxidant enzymes was recovered to a normal degree. These data imply that UCMSC-CM clearly has the potential to prevent muscle atrophy. Thus, our present study offers fundamental data on the potential treatment of muscle-related disease using UCMSC-CM.
authors.affiliation: Department of Biotechnology, CHA University, 689 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, South Korea. chanmi319@gmail.com.
authors.forename: Chan-Mi
authors.initials: CM
authors.lastname: Park
authors.affiliation: Department of Biotechnology, CHA University, 689 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, South Korea. treasure7744@naver.com.
authors.forename: Mi Jin
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, CHA University, 689 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, South Korea. glena0204@naver.com.
authors.forename: Sun-Mi
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, CHA University, 689 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, South Korea. wlsgh3142@naver.com.
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Biotechnology, CHA University, 689 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, South Korea. kimzhun@gmail.com.
authors.forename: Z-Hun
authors.initials: ZH
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, CHA University, 689 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, South Korea. yschoi@cha.ac.kr.
authors.forename: Yong-Soo
authors.initials: YS
authors.lastname: Choi
mesh_terms: D000818:Animals; D002374:Catalase / Q000096:biosynthesis; D049109:Cell Proliferation / Q000235:genetics; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003893:Desmin / Q000096:biosynthesis* / Q000235:genetics; D003907:Dexamethasone / Q000633:toxicity; D004195:Disease Models, Animal; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000096:biosynthesis; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D009124:Muscle Proteins / Q000096:biosynthesis / Q000235:genetics; D009133:Muscular Atrophy / Q000139:chemically induced / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D018008:Myogenin / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D044843:SKP Cullin F-Box Protein Ligases / Q000096:biosynthesis / Q000235:genetics; D013482:Superoxide Dismutase / Q000096:biosynthesis; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases / Q000096:biosynthesis / Q000235:genetics; D014470:Umbilical Cord / Q000166:cytology / Q000187:drug effects / Q000637:transplantation; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2
chemical_list: D002374:Catalase; D017077:Culture Media, Conditioned; D003893:Desmin; D003907:Dexamethasone; D005979:Glutathione Peroxidase; D009124:Muscle Proteins; D018008:Myogenin; D017382:Reactive Oxygen Species; D044843:SKP Cullin F-Box Protein Ligases; D013482:Superoxide Dismutase; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2; C000599875:Trim55 protein, rat; C486692:Fbxo32 protein, rat
keywords: Dexamethasone; L6 skeletal muscle cell; Muscle atrophy; Umbilical cord mesenchymal stem cell (UCMSC); Umbilical cord mesenchymal stem cell-conditioned medium (UCMSC-CM)