title: Variation in antigen-antibody affinity among serotypes of Salmonella O4 serogroup, determined using specific antisera.
abstract: Serotyping is widely used for typing Salmonella during surveillance, and depends on determining the lipopolysaccharide (LPS) O-antigen and the flagellar protein (H-antigens) components. As the O-antigen is highly variable, and structurally unique to each serotype, we investigated the binding affinities of LPS from Salmonella serotypes of O4 serogroup with specific anti-antigen serum via immunoblot and enzyme-linked immunosorbent assays. Since the serotypes from O4 serogroup also express the O-antigen factor 12, O12 antiserum was also used for the analysis. LPS from the different serotypes showed different binding affinities with the antisera. Therefore, based on the antigen-antibody affinity, a modified agglutination assay was carried out by using O4 and O12 antisera. Although serotypes from O4 serogroup have the common O-antigen factors 4 and 12, the analysis showed that the degree of agglutination reaction is different for each of the serotypes. We suggest that Salmonella serogroup O4 serotypes exhibit different binding affinities with specific antisera despite the presence of common O-antigen factors 4 and 12.
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal, Health, NARO, 3-1-5 Kannondai Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Swarmistha Devi
authors.initials: SD
authors.lastname: Aribam
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal, Health, NARO, 3-1-5 Kannondai Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Marta
authors.initials: M
authors.lastname: Elsheimer-Matulova
authors.affiliation: Kitasato Institute for Life Sciences and Graduate School of Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Matsui
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal, Health, NARO, 3-1-5 Kannondai Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Hirota
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal, Health, NARO, 3-1-5 Kannondai Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Shiraiwa
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal, Health, NARO, 3-1-5 Kannondai Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Yohsuke
authors.initials: Y
authors.lastname: Ogawa
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal, Health, NARO, 3-1-5 Kannondai Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hikono
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal, Health, NARO, 3-1-5 Kannondai Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shimoji
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal, Health, NARO, 3-1-5 Kannondai Tsukuba, Ibaraki 305-0856, Japan egumaro@affrc.go.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Eguchi
mesh_terms: D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000276:immunology*; D000915:Antibody Affinity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007106:Immune Sera; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000276:immunology / Q000378:metabolism; D019081:O Antigens / Q000737:chemistry / Q000276:immunology*; D012475:Salmonella / Q000145:classification / Q000276:immunology*; D065288:Serogroup; D012703:Serotyping
chemical_list: D000907:Antibodies, Bacterial; D007106:Immune Sera; D008070:Lipopolysaccharides; D019081:O Antigens
keywords: O-antigen; Salmonella; antigen-antibody affinity; lipopolysaccharide