title: Neutrophil CD64 is upregulated in RA patients with lymphoma but not in other solid cancers.
abstract: OBJECTIVE
  To evaluate the utility of quantifying CD64 expression on neutrophils in rheumatoid arthritis patients with malignancy, especially its diagnostic role in lymphoma.
  
  
  METHODS
  We used flow cytometry to quantify CD64 expression on neutrophils from patients diagnosed with malignancy during the follow-up period prior to initiating treatment.
  
  
  RESULTS
  Neutrophils from 18 patients with lymphoma expressed significantly higher levels of CD64 (9635.6 ± 2123.7 molecules/cell) than those from 32 patients with other solid cancers (carcinoma) (1250.5 ± 91.1 molecules/cell) (p < 0.001). When the cutoff value was set at 2060 molecules/cell, the sensitivity and specificity of CD64 for diagnosing lymphoma was 88.9% and 94.4%, respectively.
  
  
  CONCLUSIONS
  The quantitative measurement of neutrophil CD64 by flow cytometry may be useful as a subsidiary diagnostic marker in patients with suspected lymphoma. Although neutrophil CD64 is currently a well-known marker of infection, it is necessary to bear in mind that lymphoma is also a candidate in differential diagnosis when CD64 expression on neutrophils is upregulated.
authors.affiliation: a Department of Clinical Laboratory , Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan .
authors.forename: Akiko
authors.initials: A
authors.lastname: Komiya
authors.affiliation: b Department of Rheumatology , Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan .
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Matsui
authors.affiliation: b Department of Rheumatology , Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan .
authors.forename: Koichiro
authors.initials: K
authors.lastname: Horie
authors.affiliation: b Department of Rheumatology , Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan .
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Fukuda
authors.affiliation: c Department of Rheumatology , Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan .
authors.forename: Kanako
authors.initials: K
authors.lastname: Iwata
authors.affiliation: b Department of Rheumatology , Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan .
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Tsuno
authors.affiliation: b Department of Rheumatology , Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan .
authors.forename: Hideki
authors.initials: H
authors.lastname: Ogihara
authors.affiliation: d Department of Rehabilitation , Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan , and.
authors.forename: Tatsuoh
authors.initials: T
authors.lastname: Ikenaka
authors.affiliation: b Department of Rheumatology , Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan .
authors.forename: Misato
authors.initials: M
authors.lastname: Kawakami
authors.affiliation: c Department of Rheumatology , Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan .
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Furukawa
authors.affiliation: b Department of Rheumatology , Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan .
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hashimoto
authors.affiliation: e Department of Urology , Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Hirai
authors.affiliation: c Department of Rheumatology , Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan .
authors.forename: Shigeto
authors.initials: S
authors.lastname: Tohma
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000150:complications / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008223:Lymphoma / Q000150:complications / Q000175:diagnosis* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000150:complications / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D017452:Receptors, IgG / Q000378:metabolism*; D012680:Sensitivity and Specificity; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D017452:Receptors, IgG
keywords: CD64; Flow cytometry; Neutrophil; Rheumatoid arthritis; lymphoma