title: The Ru complex and hollow gold nanoparticles branched-hydrogel as signal probe for construction of electrochemiluminescent aptasensor.
abstract: In this work, a novel Ru complex and hollow gold nanoparticles branched-poly(N-(3-aminopropyl)methacrylamide) hydrogel composites (pNAMA-Ru-HGNPs) were prepared and used as electrogenerated chemiluminescence (ECL) signal probe to construct aptasensor for ultrasensitive detection of thrombin (TB). Herein, [Ru(phen)2(cpaphen)](2+) linked N-(3-aminopropyl)methacrylamide and hollow gold nanoparticles functionalized N-(3-aminopropyl)methacrylamide were used as two polymer monomers to prepare pNAMA-Ru-HGNPs composites via free-radical polymerization. The obtained hydrogel composite, containing amount of Ru complex and HGNPs, were used as effective tag-carriers for the immobilization of thrombin binding aptamer II (TBA II) to form the pNAMA-Ru-HGNPs labeled TBA II (pNAMA-Ru-HGNPs-TBA II). For building the interface of the aptasensor, dendritic gold nanoparticles reduced by poly(ethyleneimine) (PEI@DGNPs) were modified on the carbon nanotube-nafion (CNTs-Nf) coated electrode through electrostatic adsorption, which was used not only as matrix for immobilization of thrombin binding aptamer I (TBA I) but also as enhancer to amplify the ECL signal because PEI is an efficient co-reactant of Ru complex. Target TB was sandwiched between pNAMA-Ru-HGNPs-TBA II and TBA I, resulting in the ECL signals relevant to the TB concentrations. Combining the novel pNAMA-Ru-HGNPs containing amount of Ru complex as the ECL signal probe and PEI@DGNPs as the enhancer for signal amplification, the sandwich ECL aptasensor was constructed for the detection of TB with a wide range of 1.0 fM to 10 pM and a low detection of 0.54 fM.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China; College of Chemical Engineering, Guizhou University of Engineering Science, Guizhou 551700, China.
authors.forename: Guo-Feng
authors.initials: GF
authors.lastname: Gui
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: yingzhuo@swu.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Ya-Qin
authors.initials: YQ
authors.lastname: Chai
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: yuanruo@swu.edu.cn.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015336:Molecular Probe Techniques / Q000295:instrumentation; D015335:Molecular Probes / Q000138:chemical synthesis; D058608:Nanopores / Q000648:ultrastructure; D016062:Porosity; D012428:Ruthenium / Q000737:chemistry*; D013917:Thrombin / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D020100:Hydrogels; D015335:Molecular Probes; C081676:thrombin aptamer; D006046:Gold; D012428:Ruthenium; D013917:Thrombin
keywords: ECL aptasensor; Gold nanoparticles; Hydrogel; Thrombin