title: Evidence that the Malaria Parasite Plasmodium falciparum Putative Rhoptry Protein 2 Localizes to the Golgi Apparatus throughout the Erythrocytic Cycle.
abstract: Invasion of a red blood cell by Plasmodium falciparum merozoites is an essential step in the malaria lifecycle. Several of the proteins involved in this process are stored in the apical complex of the merozoite, a structure containing secretory organelles that are released at specific times during invasion. The molecular players involved in erythrocyte invasion thus represent potential key targets for both therapeutic and vaccine-based strategies to block parasite development. In our quest to identify and characterize new effectors of invasion, we investigated the P. falciparum homologue of a P. berghei protein putatively localized to the rhoptries, the Putative rhoptry protein 2 (PbPRP2). We show that in P. falciparum, the protein colocalizes extensively with the Golgi apparatus across the asexual erythrocytic cycle. Furthermore, imaging of merozoites caught at different times during invasion show that PfPRP2 is not secreted during the process instead staying associated with the Golgi apparatus. Our evidence therefore suggests that PfPRP2 is a Golgi protein and that it is likely not a direct effector in the process of merozoite invasion.
authors.affiliation: Centre de recherche en infectiologie, CHU-Université Laval, Quebec City, Quebec, Canada.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Hallée
authors.affiliation: Centre de recherche en infectiologie, CHU-Université Laval, Quebec City, Quebec, Canada.
authors.forename: Dave
authors.initials: D
authors.lastname: Richard
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D015153:Blotting, Western; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism / Q000469:parasitology*; D006056:Golgi Apparatus / Q000235:genetics / Q000378:metabolism*; D006790:Host-Parasite Interactions; D006801:Humans; D008018:Life Cycle Stages; D016778:Malaria, Falciparum / Q000469:parasitology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D052940:Merozoites / Q000254:growth & development / Q000378:metabolism* / Q000469:parasitology; D010963:Plasmodium falciparum / Q000254:growth & development* / Q000472:pathogenicity; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D015800:Protozoan Proteins; C090555:ROP 2 protein, Toxoplasma gondii; C065352:rhoptry associated protein, Plasmodium