title: [Effective Ingredients of Yangjing Zhongyu Decoction Regulated Androgen Biosyntheses by Mitogen-Activated Protein Kinase Pathway in Porcine Granulose Cells].
abstract: OBJECTIVE
  To study the molecular mechanism of Yangjing Zhongyu Decoction (YZD) n-butanol extracts (ZDC) and ethyl acetate extracts (YSYZ) in reducing androgen in porcine granulose cells by mitogen-activated protein kinase (MAPK) pathway.
  
  
  METHODS
  Porcine granulose cells were isolated and cultured. They were inoculated by MAPK inhibitor PD98059 at different concentrations, and then they were divided into the blank control group (0), 1, 3, 10, and 25 micromol/L groups. After 24-h culture the cytochrome P450c17a (CYP17) mRNA expression level was detected using Real-time fluorescent quantitative PCR. Contents of androgen (testosterone) in the supernate were detected using RIA and optimal PD98059 concentration screened. After intervened by 10 micromol/L PD98059 for 24 h, the culture solution was intervened by effective ingredients of with or without YZD or YSYZ at various concentrations (0, 1 , 5, 25, 50 mg/mL) at various time points (3, 6, 18, 24 h). Expression levels of p-ERK1/2, c-Fos and CYP17 were detected by Western blot. Testosterone content in the supernate was determined by radioimmunoassay (RIA).
  
  
  RESULTS
  Ten pLmol/L PD98059 could obviously decrease p-ERK1/2 protein expression and increase CYP17 mRMA expression, and elevate testosterone content in the supernate (P < 0.05). ZDC and YSYZ at 25 ng/mL could increase p-ERK1/2 protein expression and c-Fos levels, and reduce CYP17 protein expression, and lower testosterone content in the supernate after 6-h intervention (P < 0.01).
  
  
  CONCLUSIONS
  Effective ingredients of YZD could reduce androgen production in porcine granulose cells through increasing activities of MAPK.
authors.forename: Dan-feng
authors.initials: DF
authors.lastname: Ye
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Ma
authors.forename: Wan-ting
authors.initials: WT
authors.lastname: Mu
authors.forename: Mao-hua
authors.initials: MH
authors.lastname: Lai
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Zheng
authors.forename: Wan-ying
authors.initials: WY
authors.lastname: Ma
mesh_terms: D000728:Androgens; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005419:Flavonoids; D006107:Granulosa Cells / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D012333:RNA, Messenger; D013552:Swine
chemical_list: D000728:Androgens; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D012333:RNA, Messenger; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one