title: [Progress in co-immobilization of multiple enzymes].
abstract: Enzyme immobilization is the core technology of biocatalysis. Over the past few decades, enzyme immobilization research mainly focused on single enzyme immobilization. In recent years, multi-enzyme immobilization attracts more and more attention as it could increase the local concentration of reaction and improve the reaction yield. In this review, a summary of the recent progress, together with our research, is presented. Special emphasis is placed on four methods in multi-enzymes co-immobilization, namely, the nonspecific covalent co-immobilization, the nonspecific non-covalent co-immobilization, the non-covalent encapsulation co-immobilized and the site specificity co-immobilized. Finally, some industrial uses of immobilized multi-enzymes were addressed and the application prospect of multi-enzyme immobilization was highlighted.
authors.forename: Jindan
authors.initials: J
authors.lastname: Wang
authors.forename: Guangya
authors.initials: G
authors.lastname: Zhang
mesh_terms: D055162:Biocatalysis; D004800:Enzymes, Immobilized
chemical_list: D004800:Enzymes, Immobilized