title: Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity.
abstract: The cuticle covers the aerial parts of land plants, where it serves many important functions, including water retention. Here, a recessive cuticle mutant, eceriferum-ym (cer-ym), of Hordeum vulgare L. (barley) showed abnormally glossy spikes, sheaths, and leaves. The cer-ym mutant plant detached from its root system was hypersensitive to desiccation treatment compared with wild type plants, and detached leaves of mutant lost 41.8% of their initial weight after 1 h of dehydration under laboratory conditions, while that of the wild type plants lost only 7.1%. Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining. The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H. The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.
authors.affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences , Lanzhou 730000 , China ; National Institute of Agrobiological Sciences , Tsukuba, Ibaraki 305-8602 , Japan.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: National Institute of Agrobiological Sciences , Tsukuba, Ibaraki 305-8602 , Japan.
authors.forename: Cheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences , Lanzhou 730000 , China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Ma
authors.affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences , Lanzhou 730000 , China.
authors.forename: Aidong
authors.initials: A
authors.lastname: Wang
authors.affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences , Lanzhou 730000 , China.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Duan
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne , Lausanne CH-1015 , Switzerland.
authors.forename: Christiane
authors.initials: C
authors.lastname: Nawrath
authors.affiliation: National Institute of Agrobiological Sciences , Tsukuba, Ibaraki 305-8602 , Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Komatsuda
authors.affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences , Lanzhou 730000 , China ; National Institute of Agrobiological Sciences , Tsukuba, Ibaraki 305-8602 , Japan.
authors.forename: Guoxiong
authors.initials: G
authors.lastname: Chen
keywords: cuticle; cutin; desiccation resistance; eceriferum-mutant; genetic mapping; wax