title: Rhodanobacter aciditrophus sp. nov., an acidophilic bacterium isolated from mine wastewater.
abstract: A novel strain (designated sjH1T), characterized as aerobic, Gram-stain-negative, oxidase-positive, catalase-negative, motile and rod-shaped, was isolated from mine wastewater. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain sjH1T belonged to the genus Rhodanobacter. Strain sjH1T was closely related to Rhodanobacter thiooxydans LCS2T (98.0% 16S rRNA gene sequence similarity), Rhodanobacter denitrificans 2APBS1T (97.7%), Rhodanobacter soli DCY45T (97.2%) and Rhodanobacter caeni MJ01T (97.0%). The DNA G+C content of strain sjH1T was 69.2 mol%. DNA-DNA relatedness ( < 60%) indicated that strain sjH1T represents a distinct species that is separate from R. thiooxydans, R. denitrificans, R. soli and R. caeni. The major ubiquinone was Q-8, and major fatty acids were summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl), iso-C15 : 0, iso-C17 : 0, iso-C16 : 0 and anteiso-C15 : 0. Based on data from this polyphasic study, it is proposed that sjH1T ( = KCTC 42660T = JCM 30774T) is the type strain of a novel species, Rhodanobacter aciditrophus sp. nov.
authors.affiliation: Department of Biology, Jeju National University, Jejudaehak-ro 102, Jeju 690-756, Republic of Korea.
authors.forename: Hyeon-Woo
authors.initials: HW
authors.lastname: Koh
authors.affiliation: Department of Microbiology, Chungbuk National University, Chungbuk 361-763, Republic of Korea.
authors.forename: Heeji
authors.initials: H
authors.lastname: Hong
authors.affiliation: Department of Microbiology, Chungbuk National University, Chungbuk 361-763, Republic of Korea.
authors.forename: Ui-Gi
authors.initials: UG
authors.lastname: Min
authors.affiliation: National Institute of Biological Resources, Microorganism Resources Division, , Incheon, Republic of Korea.
authors.forename: Myung-Suk
authors.initials: MS
authors.lastname: Kang
authors.affiliation: Microbial Resource Center/KCTC, Korea Research Institute of Bioscience and Biotechnology, , 125 Gwahakro, Yuseong, Daejeon 305-806, Republic of Korea.|University of Science and Technology (UST), 217 Gajeong-ro, Yuseong, Daejeon 305-350, Republic of Korea.
authors.forename: Song-Gun
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
authors.forename: Jeong-Geol
authors.initials: JG
authors.lastname: Na
authors.affiliation: Department of Microbiology, Chungbuk National University, Chungbuk 361-763, Republic of Korea.
authors.forename: Sung-Keun
authors.initials: SK
authors.lastname: Rhee
authors.affiliation: Department of Biology, Jeju National University, Jejudaehak-ro 102, Jeju 690-756, Republic of Korea.
authors.forename: Soo-Je
authors.initials: SJ
authors.lastname: Park
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008906:Mining; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry; D062065:Wastewater / Q000382:microbiology*; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D062065:Wastewater; C030778:ubiquinone 8