title: Practical and low cost whole-organism motility assay: A step-by-step protocol.
abstract: Here, we provide a step-by-step protocol for a practical and low cost whole-organism assay for the screening of chemical compounds for activity against parasitic worms. This assay has considerable advantages over conventional methods, mainly in relation to ease of use, throughput, time and cost. It is readily suited to the screening of hundreds to thousands of compounds for subsequent hit-to-lead optimisation, and should be applicable to many different parasites and other organisms commensurate with the size of wells in the microtiter plates used for phenotypic screening.
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Sarah
authors.initials: S
authors.lastname: Preston
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Abdul
authors.initials: A
authors.lastname: Jabbar
authors.affiliation: Monash University Institute of Pharmaceutical Sciences (MIPS), Monash University, Parkville, Victoria 3052, Australia.
authors.forename: Cameron
authors.initials: C
authors.lastname: Nowell
authors.affiliation: Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinärstrasse 1, A-1210 Vienna, Austria.
authors.forename: Anja
authors.initials: A
authors.lastname: Joachim
authors.affiliation: Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinärstrasse 1, A-1210 Vienna, Austria.
authors.forename: Baerbel
authors.initials: B
authors.lastname: Ruttkowski
authors.affiliation: Monash University Institute of Pharmaceutical Sciences (MIPS), Monash University, Parkville, Victoria 3052, Australia.
authors.forename: Tony
authors.initials: T
authors.lastname: Cardno
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia; Structural Chemistry Program, Eskitis Institute, Griffith University, Brisbane, Queensland 4111, Australia.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hofmann
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address: robinbg@unimelb.edu.au.
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D003362:Cost-Benefit Analysis; D004353:Drug Evaluation, Preclinical / Q000191:economics / Q000295:instrumentation / Q000379:methods; D057166:High-Throughput Screening Assays / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D007814:Larva / Q000187:drug effects / Q000502:physiology; D008124:Locomotion / Q000187:drug effects* / Q000502:physiology; D015203:Reproducibility of Results; D017183:Strongylida / Q000187:drug effects* / Q000502:physiology
chemical_list: D000969:Antinematodal Agents
keywords: Drug screening; Motility assay; Nematode; Parasite; Step-by-step protocol