title: Analysis of UB and L40 resistance related to deltamethrin in Drosophila kc cells.
abstract: Pests have been subjected to heavy selection pressure, and the development of resistance to pyrethroid has been recorded. It is extremely valuable to identify the resistance genes that are relevant for pest control. In our previous studies, we reported that UBL40 is the deltamethrin resistance-associated gene. UBL40 is cleaved by specific endopeptidases to release UB and L40. Whether UB or L40 participates in deltamethrin resistance requires further study. In this study, quantitative real-time PCR was applied to reveal that UB and L40 were both overexpressed in Drosophila kc cells after deltamethrin stimulation. To investigate the roles of UB and L40 further, RNA interferences (RNAi) and cell transfections were utilized. UB and L40 knockdown both significantly reduced the level of resistance of RNAi-treated cells after 48 h, and the overexpressions of UB and L40 in Drosophila kc cells conferred a degree of protection against deltamethrin. These results represent the first evidence that UB and L40 both play roles in the regulation of deltamethrin resistance and that this study could help us to elucidate the resistance mechanisms and identify new target genes associated with resistance.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Chi
authors.affiliation: Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang 550009, China.
authors.forename: Fengliang
authors.initials: F
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: School of Energy and Mechanical Engineering, Nanjing Normal University, Nanjing 210042, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Hu
authors.affiliation: Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang 550009, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang 550009, China.
authors.forename: Xuejian
authors.initials: X
authors.lastname: Jing
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China. Electronic address: chengluogen@njnu.edu.cn.
authors.forename: Luogen
authors.initials: L
authors.lastname: Cheng
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D009570:Nitriles / Q000494:pharmacology*; D011722:Pyrethrins / Q000494:pharmacology*; D034622:RNA Interference
chemical_list: D029721:Drosophila Proteins; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin
keywords: CCK-8; Cell transfection; Deltamethrin; Drosophila kc cells; L40; RNA interference; UB; Western