title: Arcanobacterium pinnipediorum sp. nov., isolated from a harbour seal.
abstract: A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like, Gram-stain-positive bacterium, strain 2710T, isolated from a harbour seal. Comparative 16S rRNA gene sequence analysis showed that this bacterial strain belonged to the genus Arcanobacterium and was related most closely to the type strains of Arcanobacterium phocae (98.4 % similarity) and Arcanobacterium phocisimile (97.5 %). 16S rRNA gene sequence similarities to the type strains of other Arcanobacterium species were between 95.3 and 96.9 %. DNA-DNA hybridization values between strain 2710T and A. phocae DSM 10002T and A. phocisimile LMG 27073T were 4.7 % (reciprocal 56 %) and 23 % (reciprocal 7.7 %), respectively. The presence of the major menaquinone MK-9(H4) and a polar lipid profile with the major compounds diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside supported the affiliation of strain 2710T to the genus Arcanobacterium. The major fatty acids were C16:0, C18:1ω9c, C18:0 and C18:2ω6,9c/anteiso-C18:0. The peptidoglycan structure was of cross-linkage type A5α (l-Lys-l-Lys-d-Glu). Physiological and biochemical tests clearly distinguished the isolate from other members of the genus Arcanobacterium. Based on these tests, it is proposed that this unknown bacterium should be classified as a novel species of the genus Arcanobacterium, with the name Arcanobacterium pinnipediorum sp. nov. The type strain is 2710T ( = DSM 28752T = LMG 28298T).
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, 35392 Gießen, Germany.
authors.forename: Osama
authors.initials: O
authors.lastname: Sammra
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, 35392 Gießen, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Balbutskaya
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, 35392 Gießen, Germany.
authors.forename: Hivda
authors.initials: H
authors.lastname: Ülbegi-Mohyla
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, 35392 Gießen, Germany.
authors.forename: Samy
authors.initials: S
authors.lastname: Nagib
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, 35392 Gießen, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Lämmler
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Gießen, 35392 Gießen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Gießen, 35392 Gießen, Germany.
authors.forename: Stefanie P
authors.initials: SP
authors.lastname: Glaeser
authors.affiliation: Abteilung für Klinische Mikrobiologie und Infektionsbiologie, Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, A-1210, Wien, Austria.
authors.forename: Jan
authors.initials: J
authors.lastname: Golke
authors.affiliation: Abteilung für Klinische Mikrobiologie und Infektionsbiologie, Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, A-1210, Wien, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
authors.affiliation: Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität, 35392 Gießen, Germany.
authors.forename: Ellen
authors.initials: E
authors.lastname: Prenger-Berninghoff
authors.affiliation: Institut für Terrestrische und Aquatische Wildtierforschung, Stiftung Tierärztliche Hochschule Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: Siebert
authors.affiliation: Institut für Lebensmittelqualität und -sicherheit, Stiftung Tierärztliche Hochschule Hannover, 30173 Hannover, Germany.
authors.forename: Amir
authors.initials: A
authors.lastname: Abdulmawjood
authors.affiliation: Institut für Lebensmittelqualität und -sicherheit, Stiftung Tierärztliche Hochschule Hannover, 30173 Hannover, Germany.
authors.forename: Günter
authors.initials: G
authors.lastname: Klein
mesh_terms: D000818:Animals; D055667:Arcanobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009659:North Sea; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D046049:Phoca / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9