title: The hypotensive effect of the nitric monoxide donor Oxacom at different routs of its administration to experimental animals.
abstract: Earlier it has been found that the hypotensive drug Oxacom containing binuclear dinitrosyl iron complexes (B-DNIC) with glutathione can effectively decrease, as a nitric monooxide (NO) donor, the mean arterial pressure (МАР) in rats upon intravenous bolus injection in the form of an aqueous solution (Chazov et al., 2012). The aim of this study was to investigate the hypotensive effects of Oxacom administered to experimental rats by intravenous, intramuscular, subcutaneous, intraperitoneal, intragastric, rectal routes.MAP and heart rate (HR) were measured with the help of arterial catheters equipped with tensometric sensors. Oxacom was administered to rats at the dose of 2.0 μmole of B-DNIC/kg. The concentration of paramagnetic mononuclear protein-bound DNIC (М-DNIC) formed in the blood and tissues of various internal organs of the rat was determined by the EPR method. Upon subcutaneous, intramuscular or intraperitoneal administration of Oxacom, the maximum amplitude of the МАР decrease varies from 30% to 70%, respectively, in comparison with the corresponding parameter for the intravenously injected Oxacom. Another difference is the lack of the fast phase in the initial stage of the МАР decrease and the longer persistence of protein-bound M-DNIC formed in the circulating blood after intramuscular, subcutaneous or intraperitoneal administration of Oxacom. Thus, the NO donor Oxacom exerts pronounced hypotensive effects on rats not only upon intravenous, but also upon intramuscular, subcutaneous or intraperitoneal administration.
authors.affiliation: Russian Cardiology Research-and-Production Complex, Russian Ministry of Health, Moscow, Russia.
authors.forename: Alexander А
authors.initials: AА
authors.lastname: Тimoshin
authors.affiliation: Russian Cardiology Research-and-Production Complex, Russian Ministry of Health, Moscow, Russia.
authors.forename: Vladimir L
authors.initials: VL
authors.lastname: Lakomkin
authors.affiliation: Russian Cardiology Research-and-Production Complex, Russian Ministry of Health, Moscow, Russia.
authors.forename: Alexander А
authors.initials: AА
authors.lastname: Аbramov
authors.affiliation: Russian Cardiology Research-and-Production Complex, Russian Ministry of Health, Moscow, Russia.
authors.forename: Enno K
authors.initials: EK
authors.lastname: Ruuge
authors.affiliation: Russian Cardiology Research-and-Production Complex, Russian Ministry of Health, Moscow, Russia.
authors.forename: Valery I
authors.initials: VI
authors.lastname: Kapel'ko
authors.affiliation: Russian Cardiology Research-and-Production Complex, Russian Ministry of Health, Moscow, Russia.
authors.forename: Evgeny I
authors.initials: EI
authors.lastname: Chazov
authors.affiliation: N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia. Electronic address: vanin@polymer.chph.ras.ru.
authors.forename: Anatoly F
authors.initials: AF
authors.lastname: Vanin
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage*; D062186:Arterial Pressure / Q000187:drug effects* / Q000502:physiology; D004333:Drug Administration Routes; D005978:Glutathione / Q000008:administration & dosage* / Q000737:chemistry; D007275:Injections, Intravenous; D007501:Iron / Q000008:administration & dosage* / Q000737:chemistry; D008297:Male; D009569:Nitric Oxide; D020030:Nitric Oxide Donors / Q000008:administration & dosage* / Q000737:chemistry; D009589:Nitrogen Oxides / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000959:Antihypertensive Agents; D020030:Nitric Oxide Donors; D009589:Nitrogen Oxides; D009569:Nitric Oxide; C073661:dinitrosyl iron complex; D007501:Iron; D005978:Glutathione
keywords: Dinitrosyl iron complexes; Hypotension; Oxacom