title: Role of thioredoxin nitration in bleomycin-induced pulmonary fibrosis in rats.
abstract: Oxidant stimulation has been suggested to play an important role in the pathogenesis of idiopathic pulmonary fibrosis (IPF). Our study aimed to investigate the role and mechanisms of thioredoxin (Trx) nitration during the development of IPF. A rat model of IPF was established by intratracheal instillation of bleomycin (BLM). Male Wistar rats were randomly distributed among the control group and BLM-treated group, in which rats were intratracheally instilled with a single dose of BLM (5.0 mg/kg body mass in 1.0 mL phosphate-buffered saline). At 7 or 28 days after instillation the rats were euthanized. Histopathological and biochemical examinations were performed. The activity and protein level of thioredoxin were assessed. The thioredoxin nitration level was determined using immunoprecipitation and immunoblotting techniques. Our results demonstrated that protein tyrosine nitration increased in the BLM-treated group compared with the control group. Trx activity decreased in the BLM group compared with control group, whereas Trx expression and nitration level increased dramatically in the BLM group compared with the control group. Our results indicated that Trx nitration might be involved in the pathogenesis of IPF.
authors.affiliation: a Department of Respiratory Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: b Department of Pathology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, P.R. China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Song
authors.affiliation: c Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Tianjin Medical University, Tianjin, 300070, P.R. China.
authors.forename: Xiankui
authors.initials: X
authors.lastname: Li
authors.affiliation: d Department of Thoracic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, P.R. China.
authors.forename: Haizhou
authors.initials: H
authors.lastname: Guo
authors.affiliation: a Department of Respiratory Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, P.R. China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D004195:Disease Models, Animal; D006801:Humans; D006909:Hydroxyproline / Q000378:metabolism; D054990:Idiopathic Pulmonary Fibrosis / Q000209:etiology / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D011658:Pulmonary Fibrosis / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013879:Thioredoxins / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D009566:Nitrates; C495697:Txn1 protein, rat; D001761:Bleomycin; C002744:3-nitrotyrosine; D014443:Tyrosine; D013879:Thioredoxins; D006909:Hydroxyproline
keywords: apoptose; apoptosis; fibrose pulmonaire; nitration; pulmonary fibrosis; thioredoxin (Trx); thiorédoxine