title: Light-enhanced bioaccumulation of molybdenum by nitrogen-deprived recombinant anoxygenic photosynthetic bacterium Rhodopseudomonas palustris.
abstract: As molybdenum (Mo) is an indispensable metal for plant nitrogen metabolisms, accumulation of dissolved Mo into bacterial cells may connect to the development of bacterial fertilizers that promote plant growth. In order to enhance Mo bioaccumulation, nitrogen removal and light illumination were examined in anoxygenic photosynthetic bacteria (APB) because APB possess Mo nitrogenase whose synthesis is strictly regulated by ammonium ion concentration. In addition, an APB, Rhodopseudomonas palustris, transformed with a gene encoding Mo-responsive transcriptional regulator ModE was constructed. Mo content was most markedly enhanced by the removal of ammonium ion from medium and light illumination while their effects on other metal contents were limited. Increases in contents of trace metals including Mo by the genetic modification were observed. Thus, these results demonstrated an effective way to enrich Mo in the bacterial cells by the culture conditions and genetic modification.
authors.affiliation: a Department of Applied Biological Chemistry, Faculty of Agriculture , Utsunomiya University , Utsunomiya , Japan.
authors.forename: Taki
authors.initials: T
authors.lastname: Naito
authors.affiliation: a Department of Applied Biological Chemistry, Faculty of Agriculture , Utsunomiya University , Utsunomiya , Japan.
authors.lastname: Sachuronggui
authors.affiliation: a Department of Applied Biological Chemistry, Faculty of Agriculture , Utsunomiya University , Utsunomiya , Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Ueki
authors.affiliation: a Department of Applied Biological Chemistry, Faculty of Agriculture , Utsunomiya University , Utsunomiya , Japan.
authors.forename: Isamu
authors.initials: I
authors.lastname: Maeda
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism / Q000494:pharmacology; D000693:Anaerobiosis; D001059:Apoproteins / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering; D008027:Light; D008982:Molybdenum / Q000378:metabolism*; D008983:Molybdoferredoxin / Q000235:genetics* / Q000378:metabolism; D009584:Nitrogen / Q000172:deficiency*; D012241:Rhodopseudomonas / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D064751:Ammonium Compounds; D001059:Apoproteins; D001426:Bacterial Proteins; C101050:ModE protein, bacteria; D008983:Molybdoferredoxin; D014157:Transcription Factors; C065636:nitrogenase apomolybdenum-iron protein, Bacteria; D008982:Molybdenum; D009584:Nitrogen
keywords: ModE; molybdenum; nitrogenase; photosynthetic bacteria