title: Effects of Oral Administration of Chitin Nanofiber on Plasma Metabolites and Gut Microorganisms.
abstract: The aim of this study was to examine the effects of oral administration of chitin nanofibers (CNFs) and surface-deacetylated (SDA) CNFs on plasma metabolites using metabolome analysis. Furthermore, we determined the changes in gut microbiota and fecal organic acid concentrations following oral administrations of CNFs and SDACNFs. Healthy female mice (six-week-old) were fed a normal diet and administered tap water with 0.1% (v/v) CNFs or SDACNFs for 28 days. Oral administration of CNFs increased plasma levels of adenosine triphosphate (ATP), adenosine diphosphate (ADP), and serotonin (5-hydroxytryptamine, 5-HT). Oral administration of SDACNFs affected the metabolisms of acyl-carnitines and fatty acids. The fecal organic level analysis indicated that oral administration of CNFs stimulated and activated the functions of microbiota. These results indicate that oral administration of CNFs increases plasma levels of ATP and 5-HT via activation of gut microbiota.
authors.affiliation: Department of Clinical Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. kazu-azuma@muses.tottori-u.ac.jp.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Azuma
authors.affiliation: Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan. jastam0ment@gmail.com.
authors.forename: Ryotaro
authors.initials: R
authors.lastname: Izumi
authors.affiliation: Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan. o_o_mari11@yahoo.co.jp.
authors.forename: Mari
authors.initials: M
authors.lastname: Kawata
authors.affiliation: Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan. tomonekomame@gmail.com.
authors.forename: Tomone
authors.initials: T
authors.lastname: Nagae
authors.affiliation: Department of Clinical Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. tosaki@muses.tottori-u.ac.jp.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Osaki
authors.affiliation: Department of Clinical Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. ymurahata@muses.tottori-u.ac.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Murahata
authors.affiliation: Department of Clinical Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. tsuka@muses.tottori-u.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tsuka
authors.affiliation: Department of Clinical Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. imagawat@muses.tottori-u.ac.jp.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imagawa
authors.affiliation: Department of Clinical Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. taro@muses.tottori-u.ac.jp.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Ito
authors.affiliation: Department of Clinical Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. yokamoto@muses.tottori-u.ac.jp.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Okamoto
authors.affiliation: Division of Instrumental Analysis, Research Center for Bioscience and Technology, Tottori University, Tottori 680-8550, Japan. morimoro@chem.tottori-u.ac.jp.
authors.forename: Minoru
authors.initials: M
authors.lastname: Morimoto
authors.affiliation: Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan. h-izawa@chem.tottori-u.ac.jp.
authors.forename: Hironori
authors.initials: H
authors.lastname: Izawa
authors.affiliation: Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan. saimoto@chem.tottori-u.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Saimoto
authors.affiliation: Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan. sifuku@chem.tottori-u.ac.jp.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ifuku
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002686:Chitin / Q000008:administration & dosage*; D016000:Cluster Analysis; D004734:Energy Metabolism; D005243:Feces / Q000737:chemistry; D005260:Female; D000069196:Gastrointestinal Microbiome; D053858:Metabolic Networks and Pathways; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D051379:Mice; D057139:Nanofibers / Q000008:administration & dosage*
chemical_list: D002686:Chitin
keywords: ATP; acyl-carnitine; chitin nanofibers; fatty acids; metabolome analysis; serotonin; surface-deacetylated chitin nanofibers