title: Antioxidant and Anti-Inflammatory Activities of a Natural Compound, Shizukahenriol, through Nrf2 Activation.
abstract: Imbalance in the antioxidant defense system leads to detrimental consequences, such as neurological disorders. The Nrf2 signaling is known as a main pathway involved in cellular defense system. Nrf2 is a transcription factor that regulates oxidative stress response by inducing expression of various antioxidant enzyme genes. In this study, we screened several pure natural compounds for Nrf2 activator. Among them, shizukahenriol (SZH), isolated from Chloranthus henryi, activated Nrf2, and induced expression of the Nrf2-dependent antioxidant enzymes HO-1, GCLC, and GCLM in BV-2 microglial cells. This natural compound was also effective in suppressing production of inflammatory molecules NO, TNF-α, and inhibition of NF-κB p65 translocation to the nucleus in a dose-dependent manner. We also examined whether SZH rescued the microglial cells from oxidative stress-induced cell death. Pretreatment with SZH dose-dependently attenuated H₂O₂-induced cytotoxicity in BV-2 microglial cells. These results suggested SZH as a potential neuroprotective agent for neurological disorders.
authors.affiliation: Center for Neuro-Medicine, Korea Institute of Science and Technology, Seoul 136-791, Korea. 090749@kist.re.kr.
authors.forename: Jong-Hyun
authors.initials: JH
authors.lastname: Park
authors.affiliation: Center for Neuro-Medicine, Korea Institute of Science and Technology, Seoul 136-791, Korea. 213507@kist.re.kr.|Department of Biotechnology, Yonsei University, Seoul 120-749, Korea. 213507@kist.re.kr.
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Choi
authors.affiliation: Center for Neuro-Medicine, Korea Institute of Science and Technology, Seoul 136-791, Korea. 113506@kist.re.kr.|Department of Biotechnology, Yonsei University, Seoul 120-749, Korea. 113506@kist.re.kr.
authors.forename: Eun Ji
authors.initials: EJ
authors.lastname: Ju
authors.affiliation: Center for Neuro-Medicine, Korea Institute of Science and Technology, Seoul 136-791, Korea. anpae@kist.re.kr.|Department of Biological Chemistry, University of Science and Technology, Daejeon 305-350, Korea. anpae@kist.re.kr.
authors.forename: Ae Nim
authors.initials: AN
authors.lastname: Pae
authors.affiliation: Center for Neuro-Medicine, Korea Institute of Science and Technology, Seoul 136-791, Korea. kdpark@kist.re.kr.|Department of Biological Chemistry, University of Science and Technology, Daejeon 305-350, Korea. kdpark@kist.re.kr.
authors.forename: Ki Duk
authors.initials: KD
authors.lastname: Park
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001688:Biological Products / Q000737:chemistry*; D002460:Cell Line; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001688:Biological Products; D051267:NF-E2-Related Factor 2; D017382:Reactive Oxygen Species; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D006861:Hydrogen Peroxide
keywords: BV-2 mouse microglial cells; natural products; neuroprotection; nuclear factor-E2-related factor 2; shizukahenriol