title: Ribosomal DNA identification of Nosema/Vairimorpha in freshwater polychaete, Manayunkia speciosa, from Oregon/California and the Laurentian Great Lakes.
abstract: We examined Manayunkia speciosa individuals from the Klamath River, Oregon/California and Lake Erie, Michigan, USA for the presence of Microsporidia. We identified microsporidian spores and sequenced their SSU, ITS, and part of the LSU rDNA. Phylogenetic analysis of SSU rDNA indicated spores from both populations belonged to the Nosema/Vairimorpha clade. PCR showed an infection prevalence in Lake Erie M. speciosa of 0.6% (95% CI=0.5%, 0.7%). This represents the first known example of molecularly characterized Nosema/Vairimorpha isolates infecting a non-arthropod host.
authors.affiliation: Francis Marion University, Department of Biology, P.O. Box 100547, Florence, SC 29502, USA. Electronic address: dmalakauskas@fmarion.edu.
authors.forename: David M
authors.initials: DM
authors.lastname: Malakauskas
authors.affiliation: Francis Marion University, Department of Biology, P.O. Box 100547, Florence, SC 29502, USA. Electronic address: ealtman5808@g.fmarion.edu.
authors.forename: Emory C
authors.initials: EC
authors.lastname: Altman
authors.affiliation: Francis Marion University, Department of Biology, P.O. Box 100547, Florence, SC 29502, USA. Electronic address: smalakauskas@fmarion.edu.
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Malakauskas
authors.affiliation: Michigan State University, Department of Entomology, 288 Farm Lane Room 243, East Lansing, MI 48824, USA. Electronic address: smthiem@msu.edu.
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Thiem
authors.affiliation: U.S. Geological Survey, Great Lakes Science Center, 1451 Green Road, Ann Arbor, MI 48105, USA. Electronic address: dschloesser@usgs.gov.
authors.forename: Donald W
authors.initials: DW
authors.lastname: Schloesser
mesh_terms: D000818:Animals; D002140:California; D004271:DNA, Fungal / Q000737:chemistry; D006114:Great Lakes Region; D016818:Nosema / Q000235:genetics* / Q000302:isolation & purification; D009922:Oregon; D010802:Phylogeny; D011077:Polychaeta / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal
keywords: Annelid; Aquatic; Klamath River; Lake Erie; Microsporidia; Phylogeny