title: Association of the FCN2 Gene Single Nucleotide Polymorphisms with Susceptibility to Pulmonary Tuberculosis.
abstract: Ficolin-2 (FCN2) is an innate immune pattern recognition molecule that can activate the complement pathway, opsonophagocytosis, and elimination of the pathogens. The present study aimed to investigate the association of the FCN2 gene single nucleotide polymorphisms (SNPs) with susceptibility to pulmonary tuberculosis (TB). A total of seven SNPs in exon 8 (+6359 C>T and +6424 G>T) and in the promoter region (-986 G>A, -602 G>A, -557 A>G, -64 A>C and -4 A>G) of the FCN2 gene were genotyped using the PCR amplification and DNA sequencing methods in the healthy controls group (n = 254) and the pulmonary TB group (n = 282). The correlation between SNPs and pulmonary TB was analyzed using the logistic regression method. The results showed that there were no significant differences in the distribution of allelic frequencies of seven SNPs between the pulmonary TB group and the healthy controls group. However, the frequency of the variant homozygous genotype (P = 0.037, -557 A>G; P = 0.038, -64 A>C; P = 0.024, +6424 G>T) in the TB group was significantly lower than the control group. After adjustment for age and gender, these variant homozygous genotypes were found to be recessive models in association with pulmonary TB. In addition, -64 A>C (P = 0.047) and +6424 G>T (P = 0.03) were found to be codominant models in association with pulmonary TB. There was strong linkage disequilibrium (r2 > 0.80, P < 0.0001) between 7 SNPs except the -602 G>A site. Therefore, -557 A>G, -64 A>C and +6424 G>T SNPs of the FCN2 gene were correlated with pulmonary TB, and may be protective factors for TB. This study provides a novel idea for the prevention and control of TB transmission from a genetics perspective.
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Xu
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Department of Respiratory Medicine, The Sixth Hospital of Shaoxing, Shaoxing 312000, P.R. China.
authors.forename: Li-Liang
authors.initials: LL
authors.lastname: Wei
authors.affiliation: Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou 310013, P.R. China.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Shi
authors.affiliation: Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou 310013, P.R. China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Yu
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Chang-Ming
authors.initials: CM
authors.lastname: Liu
authors.affiliation: School of Medicine, Shaoxing University, Shaoxing 312000, P.R. China.
authors.forename: Xue-Hong
authors.initials: XH
authors.lastname: Liu
authors.affiliation: School of Laboratory Medicine, Jilin Medical College, Jilin 132013, P.R. China.
authors.forename: Xian-Min
authors.initials: XM
authors.lastname: Feng
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Ze-Peng
authors.initials: ZP
authors.lastname: Ping
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Jiang
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Zhong-Liang
authors.initials: ZL
authors.lastname: Chen
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Zhong-Jie
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Ji-Cheng
authors.initials: JC
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D005091:Exons / Q000235:genetics; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D037102:Lectins / Q000235:genetics*; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D014397:Tuberculosis, Pulmonary / Q000235:genetics*; D055815:Young Adult; D000097604:Ficolins
chemical_list: D037102:Lectins; D000097604:Ficolins