title: Intravenous Injections of Human Mesenchymal Stromal Cells Modulated the Redox State in a Rat Model of Radiation Myelopathy.
abstract: The main aim of the present study was to assess the antioxidative effects of human umbilical cord-derived mesenchymal stromal cells (UC-MSCs) in a rat model of radiation myelopathy. UC-MSCs were isolated from Wharton's jelly (WJ) of umbilical cords. An irradiated cervical spinal cord rat model (C2-T2 segment) was generated using a (60)Co irradiator to deliver 30 Gy of radiation. UC-MSCs were injected through the tail vein at 90 days, 97 days, 104 days, and 111 days after-irradiation. Histological damage was examined by cresyl violet/Nissl staining. The activities of two antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPX) in the spinal cord were measured by the biomedical assay. In addition, the levels of vascular endothelial growth factor (VEGF) and angiopoietin-2 (Ang-2) in the spinal cord were determined by ELISA methods. Multiple injections of UC-MSCs through the tail vein ameliorated neuronal damage in the spinal cord, increased the activities of the antioxidant enzymes CAT and GPX, and increased the levels of VEGF and Ang-2 in the spinal cord. Our results suggest that multiple injections of UC-MSCs via the tail vein in the rat model of radiation myelopathy could significantly improve the antioxidative microenvironment in vivo.
authors.affiliation: The First Affiliated Hospital of Liaoning Medical University, No. 2 Renmin Street, Guta District, Jinzhou, Liaoning 121000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Birth Defects and Reproductive Health of The National Health and Family Planning Commission, Chongqing Population and The Family Planning Science and Technology Research Institute, Chongqing 400020, China.
authors.forename: Lian-Bing
authors.initials: LB
authors.lastname: Li
authors.affiliation: Molecular Oncology and Epigenetics Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: Department of Oncology, The Second People's Hospital of Banan, Chongqing 400054, China.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Ren
authors.affiliation: Department of Obstetrics and Gynecology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Jia-Yan
authors.initials: JY
authors.lastname: Wu
authors.affiliation: Department of Orthopedics II, Xi'An Gao Xin Hospital, Xi'an 710075, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Oncology, The Second People's Hospital of Banan, Chongqing 400054, China.
authors.forename: Zhong-Hui
authors.initials: ZH
authors.lastname: Bao
authors.affiliation: Department of Oncology, The Second People's Hospital of Banan, Chongqing 400054, China.
authors.forename: Ji-Fan
authors.initials: JF
authors.lastname: Yang
authors.affiliation: Molecular Oncology and Epigenetics Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Department of Radiation Medicine, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Shao-Lin
authors.initials: SL
authors.lastname: Li
authors.affiliation: Key Laboratory of Birth Defects and Reproductive Health of The National Health and Family Planning Commission, Chongqing Population and The Family Planning Science and Technology Research Institute, Chongqing 400020, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.affiliation: Affiliated Hospital of The Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Hua
authors.initials: H
authors.lastname: You