title: The glycosylated flavonoids vitexin, isovitexin, and quercetrin isolated from Serjania erecta Radlk (Sapindaceae) leaves protect PC12 cells against amyloid-β25-35 peptide-induced toxicity.
abstract: The Aβ peptide-mediated toxicity participates in the neuronal death that occurs in Alzheimer's disease. The present study aims to isolate the major compounds of Serjania erecta Radlk leaves and assess whether these compounds protect PC12 cells from Aβ25-35 peptide-induced toxicity. We isolated three flavonoid glycosides with high purity: quercetrin, vitexin, and isovitexin. The Aβ25-35 peptide alone decreased the PC12 cell viability in a concentration-dependent manner, as evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay. We selected the Aβ25-35 peptide concentration of 50 µM for the experiments. Treatment of PC12 cells with the flavonoids before exposure to the Aβ25-35 peptide increased cell viability, i.e., these compounds protected the cells against Aβ25-35 peptide-induced toxicity. Vitexin promoted higher protection levels than quercetrin and isovitexin, and reduced the lactate dehydrogenase release and NO production in Aβ25-35 peptide-treated PC12 cells. Therefore, the glycosylated flavonoids that exist in S. erecta leaves, especially vitexin, protect PC12 cells from Aβ25-35 peptide-induced toxicity.
authors.affiliation: Department of Biotechnology, University of Ribeirão Preto (UNAERP), Av. Costábile Romano 2201, Ribeirão Preto, SP, 14096-900, Brazil.
authors.forename: Camila Carla
authors.initials: CC
authors.lastname: Guimarães
authors.affiliation: Department of Biotechnology, University of Ribeirão Preto (UNAERP), Av. Costábile Romano 2201, Ribeirão Preto, SP, 14096-900, Brazil.
authors.forename: Denise Dias
authors.initials: DD
authors.lastname: Oliveira
authors.affiliation: Department of Biotechnology, University of Ribeirão Preto (UNAERP), Av. Costábile Romano 2201, Ribeirão Preto, SP, 14096-900, Brazil.
authors.forename: Mayara
authors.initials: M
authors.lastname: Valdevite
authors.affiliation: Department of Biotechnology, University of Ribeirão Preto (UNAERP), Av. Costábile Romano 2201, Ribeirão Preto, SP, 14096-900, Brazil.
authors.forename: Ana Lúcia Fachin
authors.initials: AL
authors.lastname: Saltoratto
authors.affiliation: Department of Biotechnology, University of Ribeirão Preto (UNAERP), Av. Costábile Romano 2201, Ribeirão Preto, SP, 14096-900, Brazil.
authors.forename: Sarazete Izidia Vaz
authors.initials: SI
authors.lastname: Pereira
authors.affiliation: Department of Biotechnology, University of Ribeirão Preto (UNAERP), Av. Costábile Romano 2201, Ribeirão Preto, SP, 14096-900, Brazil.
authors.forename: Suzelei de Castro
authors.initials: Sde C
authors.lastname: França
authors.affiliation: Department of Biotechnology, University of Ribeirão Preto (UNAERP), Av. Costábile Romano 2201, Ribeirão Preto, SP, 14096-900, Brazil. Electronic address: apereira@unaerp.br.
authors.forename: Ana Maria Soares
authors.initials: AM
authors.lastname: Pereira
authors.affiliation: Post-Graduation Program in Agrochemistry, Federal Institute of Education, Science, and Technology of Goiás, Câmpus Rio Verde, Rod. Sul Goiana Km 01, Zona Rural, Rio Verde, P.O. Box 66, GO, 75901-970, Brazil.
authors.forename: Paulo Sérgio
authors.initials: PS
authors.lastname: Pereira
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D047310:Apigenin / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D016716:PC12 Cells; D010446:Peptide Fragments / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D029631:Sapindaceae / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); C012526:quercitrin; D009569:Nitric Oxide; D047310:Apigenin; D011794:Quercetin; C032731:vitexin; C049772:isovitexin
keywords: Flavonoid; Neuroprotection; Oxidative stress; Sejania erecta; Vitexin; β-Amyloid peptide