title: Comparison of three commercial fecal calprotectin ELISA test kits used in patients with Inflammatory Bowel Disease.
abstract: OBJECTIVE
  Fecal calprotectin is a noninvasive marker of intestinal inflammation used to distinguish between functional and organic bowel diseases and to evaluate disease activity among patients with Inflammatory Bowel Disease (IBD). The goal of this study was to compare three different ELISA tests measuring calprotectin in their accuracy to detect IBD and to distinguish between IBD patients with active or inactive disease.
  
  
  METHODS
  This study includes in total 148 fecal samples, 96 from patients with a previously confirmed IBD diagnosis and 52 from healthy controls, aged from 25 to 86 and 18 to 67 years, respectively. Disease activity in the patients was established using the following clinical activity indices: the Simple Clinical Colitis Activity Index (SCCAI), the Harvey Bradshaw Index (HBI) and the Modified Pouchitis Disease Activity Index (MPDAI). Three ELISA calprotectin tests (EK-CAL, CALPRO and HK325) were performed on fecal specimens and results compared.
  
  
  RESULTS
  The CALPRO calprotectin ELISA test was shown to have the best specificity of 96% compared to the HK325 and the EK-CAL calprotectin ELISA tests with 28% specificity and 74% specificity, respectively. A significant correlation between clinical activity indices and fecal calprotectin was found in patients with ulcerative colitis (p < 0.05***) and in patients with an ileoanal pouch (p < 0.05), but not in patients with Crohn's disease (p = 0.11).
  
  
  CONCLUSIONS
  The CALPRO ELISA test performed best in measuring calprotectin in feces.
authors.affiliation: a Department of Microbiology and Infection Control , Statens Serum Institut , Copenhagen , Denmark .|b Department of Biology , University of Copenhagen , Copenhagen , Denmark .
authors.forename: Hengameh Chloé
authors.initials: HC
authors.lastname: Mirsepasi-Lauridsen
authors.affiliation: c Department of Clinical Biochemistry, Immunology and Genetics , Statens Serum Institut , Copenhagen , Denmark .
authors.forename: Ulla
authors.initials: U
authors.lastname: Bachmann Holmetoft
authors.affiliation: d Department of Gastroenterology , Hvidovre University Hospital , Hvidovre , Denmark , and.
authors.forename: Sofie
authors.initials: S
authors.lastname: Ingdam Halkjær
authors.affiliation: a Department of Microbiology and Infection Control , Statens Serum Institut , Copenhagen , Denmark .
authors.forename: Karen
authors.initials: K
authors.lastname: Angeliki Krogfelt
authors.affiliation: d Department of Gastroenterology , Hvidovre University Hospital , Hvidovre , Denmark , and.|e Department of Clinical Microbiology , Hvidovre University Hospital , Hvidovre , Denmark.
authors.forename: Andreas
authors.initials: A
authors.lastname: Munk Petersen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000032:analysis; D003093:Colitis, Ulcerative / Q000175:diagnosis* / Q000601:surgery; D039021:Colonic Pouches; D003424:Crohn Disease / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D005243:Feces / Q000737:chemistry*; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D039841:Leukocyte L1 Antigen Complex / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D011933:Reagent Kits, Diagnostic; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D015415:Biomarkers; D039841:Leukocyte L1 Antigen Complex; D011933:Reagent Kits, Diagnostic
keywords: CALPRO; Calprotectin cutoff value; Crohn’s disease; inflammation in inflammatory bowel disease; pouchitis; ulcerative colitis.