title: 1H, 15N and 13C assignment of the amyloidogenic protein medin using fast-pulsing NMR techniques.
abstract: Thirty-one proteins are known to form extracellular fibrillar amyloid in humans. Molecular information about many of these proteins in their monomeric, intermediate or fibrillar form and how they aggregate and interact to form the insoluble fibrils is sparse. This is because amyloid proteins are notoriously difficult to study in their soluble forms, due to their inherent propensity to aggregate. Using recent developments in fast NMR techniques, band-selective excitation short transient and band-selective optimized flip-angle short-transient heteronuclear multiple quantum coherence we have been able to assign a 5 kDa full-length amyloidogenic protein called medin. Medin is the key protein component of the most common form of localised amyloid with a proposed role in aortic aneurysm and dissection. This assignment will now enable the study of the early interactions that could influence initiation and progression of medin aggregation. The chemical shifts have been deposited in the BioMagRes-Bank accession Nos. 25399 and 26576.
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK.
authors.forename: H A
authors.initials: HA
authors.lastname: Davies
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK.
authors.forename: M M
authors.initials: MM
authors.lastname: Phelan
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK. j.madine@liv.ac.uk.
authors.forename: J
authors.identifier: http://orcid.org/0000-0001-9963-5871
authors.initials: J
authors.lastname: Madine
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D000954:Antigens, Surface / Q000737:chemistry*; D002247:Carbon Isotopes; D006801:Humans; D008894:Milk Proteins / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D014316:Tritium
chemical_list: D000682:Amyloid; D000954:Antigens, Surface; D002247:Carbon Isotopes; C098942:MFGE8 protein, human; D008894:Milk Proteins; D009587:Nitrogen Isotopes; D014316:Tritium
keywords: Amyloid; Cardiovascular; Fast-pulsing NMR; Fibril; Medin; Resonance assignment