title: Characterization of a novel full-length bovine endogenous retrovirus, BERV-β1.
abstract: Recent studies have suggested that certain classes of endogenous retroviruses (ERVs) may be present in cattle. The aim of this study was increase the scope of knowledge regarding bovine ERVs. The ovine ERV β1 pro/pol sequence was used to design a primer set for polymerase chain reaction (PCR) amplification of a similar sequence in the bovine genome. Through phylogenetic and bioinformatic analysis of the PCR product sequence together with its flanking region, a sequence 8107 bp in length was characterized. This sequence had a typical 5'-LTR-gag-pro-pol-env-LTR-3' organization, and phylogenetic investigation defined it as a bovine ERV β1. Thus, we were able to identify a novel bovine endogenous retrovirus element.
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, via Salvemini 1, 06126, Perugia, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Torresi
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, via Salvemini 1, 06126, Perugia, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Casciari
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, via Salvemini 1, 06126, Perugia, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Giammarioli
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, via Salvemini 1, 06126, Perugia, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Feliziani
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, via Salvemini 1, 06126, Perugia, Italy. gm.demia@izsum.it.
authors.forename: Gian Mario
authors.initials: GM
authors.lastname: De Mia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics / Q000821:virology*; D020077:Endogenous Retroviruses / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D020079:Terminal Repeat Sequences; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: BERV; Bovine; Endogenous retroviruses; Genetic characterization; Phylogenetic analysis