title: The Role of Magnesium Ion Substituted Biphasic Calcium Phosphate Spherical Micro-Scaffolds in Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells.
abstract: This study was investigated the role of magnesium (Mg2+) ion substituted biphasic calcium phosphate (Mg-BCP) spherical micro-scaffolds in osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs). Mg-BCP micro-scaffolds with spherical morphology were successfully prepared using in situ co-precipitation and spray drying atomization process. The in vitro cell proliferation and differentiation of hAT-MSCs were determined up to day 14. After in vitro biological tests, Mg-BCP micro-scaffolds with hAT-MSCs showed more enhanced osteogenicity than pure hAT-MSCs as control group by unique biodegradation of TCP phase and influence of substituted Mg2+ ion in biphasic nanostructure. Therefore, these results suggest that Mg-BCP micro-scaffolds promote osteogenic differentiation of hAT-MSCs.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Keun-Koo
authors.initials: KK
authors.lastname: Shin
authors.forename: Jin Sup
authors.initials: JS
authors.lastname: Jung
authors.forename: Ho Hwan
authors.initials: HH
authors.lastname: Chun
authors.forename: Seong Soo
authors.initials: SS
authors.lastname: Park
authors.forename: Jong Kook
authors.initials: JK
authors.lastname: Lee
authors.forename: Hong-Chae
authors.initials: HC
authors.lastname: Park
authors.forename: Seog-Young
authors.initials: SY
authors.lastname: Yoon
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D018786:Bone Substitutes / Q000138:chemical synthesis; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D007477:Ions; D008274:Magnesium / Q000737:chemistry*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D010316:Particle Size; D013499:Surface Properties; D054457:Tissue Scaffolds
chemical_list: D018786:Bone Substitutes; D006882:Hydroxyapatites; D007477:Ions; C074950:hydroxyapatite-beta tricalcium phosphate; D008274:Magnesium