title: Real-time monitoring of phenazines excretion in Pseudomonas aeruginosa microbial fuel cell anode using cavity microelectrodes.
abstract: Phenazines are a kind of metabolites that can mediate extracellular Pseudomonas aeruginosa (P. aeruginosa) cells in microbial fuel cells (MFCs). However, it is still not clear that whether and how the excretion profile of phenazines is affected by the operating MFC. Here, we report a real time analysis approach based on a cavity microelectrode electrochemical sensor to investigate the phenazines excretion behavior of P. aeruginosa during MFC operation. The phenazine concentration increases at first 72 h, reaches a plateau and decreases after 120 h and also shows local dependent variation. It is dependent on the MFC current generation profile but also affect by the biofilm formation. Accordingly, a mechanism about phenazines excretion in MFC anode and the phenazines mediated extracellular electron transfer of the P. aeruginosa anode is proposed. This work provides a novel strategy for self-mediated extracellular electron transfer analysis in the operating MFCs.
authors.affiliation: Chongqing Key Laboratory for Advanced Materials & Technologies of Clean Energies, Southwest University, Chongqing 400715, China; Faculty of Materials & Energy, Southwest University, Chongqing 400715, China. Electronic address: yanqiao@swu.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Chongqing Key Laboratory for Advanced Materials & Technologies of Clean Energies, Southwest University, Chongqing 400715, China; Faculty of Materials & Energy, Southwest University, Chongqing 400715, China.
authors.forename: Ya-Juan
authors.initials: YJ
authors.lastname: Qiao
authors.affiliation: Chongqing Key Laboratory for Advanced Materials & Technologies of Clean Energies, Southwest University, Chongqing 400715, China; Faculty of Materials & Energy, Southwest University, Chongqing 400715, China.
authors.forename: Long
authors.initials: L
authors.lastname: Zou
authors.affiliation: Chongqing Key Laboratory for Advanced Materials & Technologies of Clean Energies, Southwest University, Chongqing 400715, China; Faculty of Materials & Energy, Southwest University, Chongqing 400715, China.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Ma
authors.affiliation: Chongqing Key Laboratory for Advanced Materials & Technologies of Clean Energies, Southwest University, Chongqing 400715, China; Faculty of Materials & Energy, Southwest University, Chongqing 400715, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Liu
mesh_terms: D004579:Electron Transport; D008839:Microelectrodes; D010619:Phenazines / Q000032:analysis*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
chemical_list: D010619:Phenazines
keywords: Cavity microelectrode; Microbial fuel cells; Phenazine excretion; Real time analysis