title: The E3 ubiquitin ligase activity of RING1B is not essential for early mouse development.
abstract: Polycomb-repressive complex 1 (PRC1) and PRC2 maintain repression at many developmental genes in mouse embryonic stem cells and are required for early development. However, it is still unclear how they are targeted and how they function. We show that the ability of RING1B, a core component of PRC1, to ubiquitinate histone H2A is dispensable for early mouse embryonic development and much of the gene repression activity of PRC1. Our data support a model in which PRC1 and PRC2 reinforce each other's binding but suggest that the key functions of PRC1 lie beyond the enzymatic capabilities of RING1B.
authors.affiliation: Medical Research Council Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH42XU, United Kingdom.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Illingworth
authors.affiliation: Medical Research Council Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH42XU, United Kingdom.
authors.forename: Michael
authors.initials: M
authors.lastname: Moffat
authors.affiliation: Medical Research Council Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH42XU, United Kingdom.
authors.forename: Abigail R
authors.initials: AR
authors.lastname: Mann
authors.affiliation: Medical Research Council Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH42XU, United Kingdom.
authors.forename: David
authors.initials: D
authors.lastname: Read
authors.affiliation: Medical Research Council Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH42XU, United Kingdom.
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Hunter
authors.affiliation: Medical Research Council Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH42XU, United Kingdom.
authors.forename: Madapura M
authors.initials: MM
authors.lastname: Pradeepa
authors.affiliation: Medical Research Council Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH42XU, United Kingdom.
authors.forename: Ian R
authors.initials: IR
authors.lastname: Adams
authors.affiliation: Medical Research Council Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH42XU, United Kingdom.
authors.forename: Wendy A
authors.initials: WA
authors.lastname: Bickmore
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000196:embryology / Q000201:enzymology; D018507:Gene Expression Regulation, Developmental; D006657:Histones / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D000066450:Mouse Embryonic Stem Cells / Q000201:enzymology; D009154:Mutation; D063150:Polycomb Repressive Complex 1 / Q000235:genetics* / Q000378:metabolism*; D063151:Polycomb Repressive Complex 2 / Q000378:metabolism; D011485:Protein Binding; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D006657:Histones; D063151:Polycomb Repressive Complex 2; D063150:Polycomb Repressive Complex 1; C489842:Rnf2 protein, mouse; D044767:Ubiquitin-Protein Ligases
keywords: H3K27me3; PRC1; Polycomb; embryonic stem cell; histone ubiquitination