title: Temporal kinetics of the transcriptional response to carbon depletion and sucrose readdition in Arabidopsis seedlings.
abstract: To investigate whether the transcriptional response to carbon (C) depletion and sucrose resupply depends on the duration and severity of the C depletion, Arabidopsis seedlings were grown in liquid culture and harvested 3, 6, 12, 24, 48 and 72 h after removing sucrose from the medium and 30 min after resupplying sucrose at each time. Expression profiling revealed early transcriptional inhibition of cell wall synthesis and remodelling of signalling, followed by induction of C recycling and photosynthesis and general inhibition of growth. The temporal sequence differed from the published response to progressive exhaustion of C during a night and extended night in vegetatively growing plants. The response to sucrose readdition was conserved across the C-depletion time course. Intriguingly, the vast majority of rapidly responding transcripts decreased rather than increased. The majority of transcripts that respond rapidly to sucrose and many transcripts that respond during C depletion also decrease after treating seedlings with the transcriptional inhibitor cordycepin A. Comparison with published responses to overexpression of otsA, AKIN10 and bZIP11 revealed that many genes that respond to C depletion, and especially sucrose resupply, respond to one or more of these C-signalling components. Thus, multiple factors contribute to C responsiveness, including many signalling components, transcriptional regulation and transcript turnover.
authors.affiliation: INRA, ISVV, EGFV, UMR 1287, Villenave d'Ornon, F-33140, France.|Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm, 14476, Germany.
authors.forename: Sarah Jane
authors.initials: SJ
authors.lastname: Cookson
authors.affiliation: Department of Biological Sciences, University of North Texas, 1155 Union Circle #305220, Denton, TX, 76203-5017, USA.|Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm, 14476, Germany.
authors.forename: Umesh Prasad
authors.initials: UP
authors.lastname: Yadav
authors.affiliation: Targenomix GmbH, Am Mühlenberg 11, Potsdam-Golm, 14476, Germany.|Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm, 14476, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Klie
authors.affiliation: Instituto de Recursos Naturales y Agrobiología de Salamanca, CSIC, Apartado 257, Salamanca, 37071, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Morcuende
authors.affiliation: Lehrstuhl für Botanik und Institut für Biologie I, RWTH Aachen, Worringer Weg 1, Aachen, 52062, Germany.|Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm, 14476, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Usadel
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm, 14476, Germany.
authors.forename: John Edward
authors.initials: JE
authors.lastname: Lunn
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm, 14476, Germany.
authors.forename: Mark
authors.initials: M
authors.lastname: Stitt
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000494:pharmacology*; D016000:Cluster Analysis; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D063990:Gene Ontology; D017343:Genes, Plant; D007700:Kinetics; D055442:Metabolome / Q000187:drug effects / Q000235:genetics; D008954:Models, Biological; D011401:Promoter Regions, Genetic / Q000235:genetics; D020871:RNA Stability / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics*; D013395:Sucrose / Q000494:pharmacology*; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D012333:RNA, Messenger; D013395:Sucrose; D002244:Carbon
keywords: Arabidopsis seedlings; carbohydrate starvation; sucrose readdition; transcriptional responses