title: Draft genome sequence of marine-derived Streptomyces sp. TP-A0598, a producer of anti-MRSA antibiotic lydicamycins.
abstract: Streptomyces sp. TP-A0598, isolated from seawater, produces lydicamycin, structurally unique type I polyketide bearing two nitrogen-containing five-membered rings, and four congeners TPU-0037-A, -B, -C, and -D. We herein report the 8 Mb draft genome sequence of this strain, together with classification and features of the organism and generation, annotation and analysis of the genome sequence. The genome encodes 7,240 putative ORFs, of which 4,450 ORFs were assigned with COG categories. Also, 66 tRNA genes and one rRNA operon were identified. The genome contains eight gene clusters involved in the production of polyketides and nonribosomal peptides. Among them, a PKS/NRPS gene cluster was assigned to be responsible for lydicamycin biosynthesis and a plausible biosynthetic pathway was proposed on the basis of gene function prediction. This genome sequence data will facilitate to probe the potential of secondary metabolism in marine-derived Streptomyces.
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan.
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Komaki
authors.affiliation: NBRC, Tokyo, Japan.
authors.forename: Natsuko
authors.initials: N
authors.lastname: Ichikawa
authors.affiliation: NBRC, Tokyo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Hosoyama
authors.affiliation: NBRC, Tokyo, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Fujita
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Toyama, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Igarashi
keywords: Biosynthetic gene; Lydicamycin; Polyketide synthase; Streptomyces; TPU-0037