title: HIV-1 Tat Protein Enhances Expression and Function of Breast Cancer Resistance Protein.
abstract: ATP binding cassette (ABC) transporters can transfer a variety of antiviral agents from the cytoplasm to body fluid, which results in a reduced intracellular concentration of the drugs. Proteins of HIV-1, e.g., Tat and gp120, altered some types of ABC transporter expression in brain microvascular endothelial cells and astrocytes. However, the effect of Tat on ABC transporters in T lymphocytes is unclear. In this study the status of breast cancer resistance protein (BCRP) in Tat expressing cell lines was examined with real-time PCR and flow cytometry. It was found that HIV-1 Tat protein upregulated BCRP expression and enhanced efflux mediated by BCRP significantly, which could inhibit antiviral drugs from entering infected cells and interfere with the therapeutic effect of HAART.
authors.affiliation: School of Pharmacy, Nankai University , Tianjin, China .
authors.forename: Yancong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Pharmacy, Nankai University , Tianjin, China .
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Nankai University , Tianjin, China .
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Yin
authors.affiliation: School of Pharmacy, Nankai University , Tianjin, China .
authors.forename: Qichang
authors.initials: Q
authors.lastname: Nie
authors.affiliation: School of Pharmacy, Nankai University , Tianjin, China .
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2 / Q000819:agonists* / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001562:Benzimidazoles / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D054659:Diketopiperazines / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D005434:Flow Cytometry; D015870:Gene Expression; D015497:HIV-1 / Q000737:chemistry / Q000235:genetics; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D019169:Jurkat Cells; D009363:Neoplasm Proteins / Q000819:agonists* / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D019076:Transgenes; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000235:genetics* / Q000378:metabolism
chemical_list: C541506:3-(6-isobutyl-9-methoxy-1,4-dioxo-1,2,3,4,6,7,12,12a-octahydropyrazino(1',2'-1,6)pyrido(3,4-b)indol-3-yl)propionic acid tert-butyl ester; C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D000903:Antibiotics, Antineoplastic; D001562:Benzimidazoles; D054659:Diketopiperazines; D006576:Heterocyclic Compounds, 4 or More Rings; D009363:Neoplasm Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D004317:Doxorubicin; C017807:bisbenzimide ethoxide trihydrochloride