title: An eDNA assay for Irish Petromyzon marinus and Salmo trutta and field validation in running water.
abstract: This pilot study presents an environmental DNA (eDNA) assay for sea lamprey Petromyzon marinus and brown trout Salmo trutta, two species of economic and conservation importance in the Republic of Ireland. The results demonstrate the effectiveness of eDNA for assessing presence of low-abundance taxa (here, P. marinus) for environmental managers, and they highlight the potential for assessing relative abundance of rare or invasive freshwater species.
authors.affiliation: Area 52 Research Group, School of Biology & Environment Science and Earth Institute, University College Dublin, Belfield, Dublin, Ireland.
authors.forename: M S
authors.initials: MS
authors.lastname: Gustavson
authors.affiliation: Area 52 Research Group, School of Biology & Environment Science and Earth Institute, University College Dublin, Belfield, Dublin, Ireland.
authors.forename: P C
authors.initials: PC
authors.lastname: Collins
authors.affiliation: Area 52 Research Group, School of Biology & Environment Science and Earth Institute, University College Dublin, Belfield, Dublin, Ireland.
authors.forename: J A
authors.initials: JA
authors.lastname: Finarelli
authors.affiliation: Area 52 Research Group, School of Biology & Environment Science and Earth Institute, University College Dublin, Belfield, Dublin, Ireland.
authors.forename: D
authors.initials: D
authors.lastname: Egan
authors.affiliation: Inland Fisheries Ireland, Shannon RBD Head Office, Dock Road, Limerick, Ireland.
authors.forename: R Ó
authors.initials: RÓ
authors.lastname: Conchúir
authors.affiliation: Inland Fisheries Ireland, 3044 Lake Drive, Dublin, 24, Ireland.
authors.forename: G D
authors.initials: GD
authors.lastname: Wightman
authors.affiliation: Inland Fisheries Ireland, 3044 Lake Drive, Dublin, 24, Ireland.
authors.forename: J J
authors.initials: JJ
authors.lastname: King
authors.affiliation: Department of Biological Sciences, Old Dominion University, Norfolk, VA, 23529, U.S.A.
authors.forename: D T
authors.initials: DT
authors.lastname: Gauthier
authors.affiliation: School of Biology & Environment Science, University College Dublin, Belfield, Dublin, Ireland.
authors.forename: K
authors.initials: K
authors.lastname: Whelan
authors.affiliation: Area 52 Research Group, School of Biology & Environment Science and Earth Institute, University College Dublin, Belfield, Dublin, Ireland.
authors.forename: J E L
authors.initials: JE
authors.lastname: Carlsson
authors.affiliation: Area 52 Research Group, School of Biology & Environment Science and Earth Institute, University College Dublin, Belfield, Dublin, Ireland.
authors.forename: J
authors.initials: J
authors.lastname: Carlsson
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis*; D056727:Endangered Species; D005618:Fresh Water / Q000032:analysis*; D007494:Ireland; D048428:Petromyzon / Q000235:genetics*; D010865:Pilot Projects; D014337:Trout / Q000235:genetics*
chemical_list: D004247:DNA
keywords: brown trout; eDNA; qPCR; sea lamprey; threatened species