title: Androgen levels and apoptosis in the testis during postnatal development of finasteride-treated male rat offspring.
abstract: BACKGROUND
  The hormone-dependent events that occur throughout the first wave of spermatogenesis, such as the establishment of the number of Sertoli cells (SCs) and spermatogonial stem cells (SSCs) within the seminiferous cords and the setting up of the blood-testis barrier, are important for adult male fertility. Any changes in the T/DHT ratio can result in male subfertility or even infertility. In this study we aimed to evaluate effects of paternal exposure to 5-alpha reductase type 2 inhibitor, finasteride on litter size, androgen levels and germ cell apoptosis in male offspring during postnatal development.
  
  
  METHODS
  The subjects of the study were 7, 14, 21/22, 28, and 90-day-old Wistar male rats (F1:Fin) born from females fertilized by finasteride-treated rats. Offspring born from untreated parental animals were used as a control group (F1:Control). Animals and the collected testes were weighed, blood and intratesticular levels of T and DHT were measured by ELISA, and the apoptotic index of testicular cells was evaluated by TUNEL technique.
  
  
  RESULTS
  We observed difficulties in obtaining male newborns from female rats fertilized by finasteride-treated male rats. In the F1:Fin rats, changes in the body and testes weights occurred, and a lower number of apoptotic cells was found during postnatal maturation of the seminiferous epithelium. Changes in androgen concentrations during the first spermatogenesis wave and adult life were also evident.
  
  
  CONCLUSIONS
  Finasteride treatment of male adult rats may not only cause a decrease in the fertility of parental rats, but also could lead to incorrect, androgen-sensitive course of spermatogenesis in their offspring.
authors.affiliation: Department of Histology and Embryology, Pomeranian Medical University, Szczecin, Poland. agnieszka.kolasa@pum.edu.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kolasa-Wolosiuk
authors.forename: Kamila
authors.initials: K
authors.lastname: Misiakiewicz-Has
authors.forename: Irena
authors.initials: I
authors.lastname: Baranowska-Bosiacka
authors.forename: Izabela
authors.initials: I
authors.lastname: Gutowska
authors.forename: Barbara
authors.initials: B
authors.lastname: Wiszniewska
mesh_terms: D000728:Androgens / Q000097:blood*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects; D005260:Female; D018120:Finasteride / Q000494:pharmacology / Q000633:toxicity*; D006801:Humans; D007223:Infant; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000728:Androgens; D018120:Finasteride
keywords: TUNEL; finasteride treatment; germ-cell apoptosis; male rats; offspring androgen levels