title: Development of engineered Escherichia coli whole-cell biocatalysts for high-level conversion of L-lysine into cadaverine.
abstract: A whole-cell biocatalytic system for the production of cadaverine from L-lysine has been developed. Among the investigated lysine decarboxylases from different microorganisms, Escherichia coli LdcC showed the best performance on cadaverine synthesis when E. coli XL1-Blue was used as the host strain. Six different strains of E. coli expressing E. coli LdcC were investigated and recombinant E. coli XL1-Blue, BL21(DE3) and W were chosen for further investigation since they showed higher conversion yield of lysine into cadaverine. The effects of substrate pH, substrate concentrations, buffering conditions, and biocatalyst concentrations have been investigated. Finally, recombinant E. coli XL1-Blue concentrated to an OD(600) of 50, converted 192.6 g/L (1317 mM) of crude lysine solution, obtained from an actual lysine manufacturing process, to 133.7 g/L (1308 mM) of cadaverine with a molar yield of 99.90 %. The whole-cell biocatalytic system described herein is expected to be applicable to the development of industrial bionylon production process.
authors.affiliation: Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-600, Republic of Korea.
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Oh
authors.affiliation: Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-600, Republic of Korea.
authors.forename: Kyoung-Hee
authors.initials: KH
authors.lastname: Kang
authors.affiliation: Department of Environmental Engineering and Energy, Myongji University, 116 Myongji-ro, Cheoin-gu, Yongin, Gyeonggido, 449-728, Republic of Korea.
authors.forename: Mi Jeong
authors.initials: MJ
authors.lastname: Kwon
authors.affiliation: Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-600, Republic of Korea.|Department of Biological and Chemical Engineering, Hongik University, Chungnam, 339-701, Republic of Korea.
authors.forename: Jae Woo
authors.initials: JW
authors.lastname: Choi
authors.affiliation: Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-600, Republic of Korea.
authors.forename: Jeong Chan
authors.initials: JC
authors.lastname: Joo
authors.affiliation: Department of Biotechnology and Bioengineering, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 500-757, Republic of Korea.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Yung-Hun
authors.initials: YH
authors.lastname: Yang
authors.affiliation: Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-600, Republic of Korea.
authors.forename: Bong Keun
authors.initials: BK
authors.lastname: Song
authors.affiliation: Bio R&D Center, Paik Kwang Industry Co., 57 Oehang-4 Gil, Gunsan-si, Jeollabukdo, Republic of Korea.
authors.forename: Il-Kwon
authors.initials: IK
authors.lastname: Kim
authors.affiliation: Bio R&D Center, Paik Kwang Industry Co., 57 Oehang-4 Gil, Gunsan-si, Jeollabukdo, Republic of Korea.
authors.forename: Ki-Hoon
authors.initials: KH
authors.lastname: Yoon
authors.affiliation: Department of Biological and Chemical Engineering, Hongik University, Chungnam, 339-701, Republic of Korea. pkm2510@hongik.ac.kr.
authors.forename: Kyungmoon
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Environmental Engineering and Energy, Myongji University, 116 Myongji-ro, Cheoin-gu, Yongin, Gyeonggido, 449-728, Republic of Korea. parksj93@mju.ac.kr.
authors.forename: Si Jae
authors.initials: SJ
authors.lastname: Park
mesh_terms: D055162:Biocatalysis; D002021:Buffers; D002103:Cadaverine / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000378:metabolism*
chemical_list: D002021:Buffers; D029968:Escherichia coli Proteins; D002262:Carboxy-Lyases; C021520:lysine decarboxylase; D008239:Lysine; D002103:Cadaverine
keywords: Bio-catalyst; Cadaverine; L-Lysine; Recombinant E. coli; Whole-cell conversion