title: Isoliquiritigenin prevents the progression of psoriasis-like symptoms by inhibiting NF-κB and proinflammatory cytokines.
abstract: Isoliquiritigenin (ISL) is an important flavonoid component of licorice and has been reported to possess anti-inflammatory and antioxidant properties, but its exact mechanism of action remains poorly understood. Previously, we demonstrated that ISL could suppress IL-6 expression in multiple myeloma. Here, we further characterized the anti-inflammatory effects of ISL in several psoriasis models, including the keratin 14/vascular endothelial growth factor (VEGF) transgenic mouse, the imiquimod (IMQ)-induced psoriasis-like mouse, and the human keratinocytes HaCaT and NHEK in vitro. We found that ISL ameliorated the inflammatory process in psoriasis models but not in their respective controls. Moreover, the anti-inflammatory effects of ISL were attributed to the suppression of nuclear factor-κB (NF-κB) activity, which consequently resulted in the reduction of pro-inflammation cytokines IL-6 and IL-8 expression. In conclusion, ISL exhibited anti-inflammatory effects in psoriasis models, by downregulating IL-6 and IL-8 via suppression of NF-κB activity, suggesting that ISL might serve as a potential candidate for treatment of psoriasis and other autoimmune inflammatory diseases.
  
  
  CONCLUSIONS
  ISL could ameliorate the inflammatory process of psoriasis. ISL could suppress NF-κB and subsequent production of a series of pro-inflammatory cytokines. Dual-inhibitory activity against IL-6 and IL-8 of ISL is implemented via inhibiting NF-κB. ISL exerts no inhibitory effects on normal human keratinocytes or wild-type Balb/c mice, implying its low toxicity and safety.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Yangping
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Xiangzheng
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China.|Department of Laboratory Medicine, First Affiliated Hospital of Zun Yi Medical College, Zun Yi Medical College, Zun Yi, 563003, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Siyuan
authors.initials: S
authors.lastname: Su
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Tinghan
authors.initials: T
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Mingtian
authors.initials: M
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Hang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China.
authors.forename: Lantu
authors.initials: L
authors.lastname: Gou
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China.|Department of Laboratory Medicine, First Affiliated Hospital of Zun Yi Medical College, Zun Yi Medical College, Zun Yi, 563003, China.|Department of Dermatology, West China Hospital, Sichuan University, Chengdu, 610041, China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China. jennyxianj@163.com.|Department of Dermatology, West China Hospital, Sichuan University, Chengdu, 610041, China. jennyxianj@163.com.
authors.forename: Xian
authors.initials: X
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China. jinliangyang@scu.edu.cn.
authors.forename: Jinliang
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D015415:Biomarkers; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D047188:Chalcones / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D018450:Disease Progression; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000378:metabolism*; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D016328:NF-kappa B / Q000378:metabolism*; D011565:Psoriasis / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D012867:Skin / Q000276:immunology / Q000378:metabolism / Q000473:pathology
chemical_list: D015415:Biomarkers; D047188:Chalcones; D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; C040920:isoliquiritigenin
keywords: Cytokines; Isoliquiritigenin; NF-κB; Psoriasis