title: Cd-induced oxidative stress and lignification in the roots of two Vicia sativa L. varieties with different Cd tolerances.
abstract: We examined the effects of Cd on growth, lipid peroxidation, reactive oxygen species (ROS) accumulation, antioxidant enzymatic activity, and lignin content in the roots of two varieties of Vicia sativa. Treatment with Cd decreased plant growth and increased ROS and lipid peroxidation levels to a greater extent in the Cd-sensitive variety ZM than in the Cd-tolerant variety L3. Most hydrogen peroxide (H2O2) and superoxide anion (O2(•-)) were accumulated in the cell walls and extracellular spaces in response to Cd treatments. Chemical assays and experiments using inhibitors showed that larger increases in H2O2 and O2(•-) production in ZM than in L3 were probably attributed to elevated Cd-induced nicotinamide adenine dinucleotide-peroxidase (NADH-POD) activity. Cd treatment increased the accumulation of lignin and the guaiacol peroxidase (GPOD) activities in the apoplast more significantly in ZM root than in L3. Howerver, root laccase activity was higher in L3 than in ZM. Thus Cd toxicity induced significant lignification in the roots of V. sativa, and increases in H2O2 accumulation and apoplastic GPOD activity were likely responsible for this effect.
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China; Taizhou University, Taizhou 225300, People's Republic of China.
authors.forename: Haiyun
authors.initials: H
authors.lastname: Rui
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China. Electronic address: zgshen@njau.edu.cn.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Shen
authors.affiliation: College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, People's Republic of China. Electronic address: fenqinzg@hxu.edu.cn.
authors.forename: Fenqin
authors.initials: F
authors.lastname: Zhang
mesh_terms: D002104:Cadmium / Q000493:pharmacokinetics / Q000633:toxicity*; D004361:Drug Tolerance; D006861:Hydrogen Peroxide / Q000378:metabolism; D042845:Laccase / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D033022:Vicia sativa / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D002104:Cadmium; D013481:Superoxides; D008031:Lignin; D006861:Hydrogen Peroxide; D042845:Laccase; C121048:guaiacol peroxidase; D009195:Peroxidase
keywords: Cadmium; Hydrogen peroxide; Lignification; Peroxidase; Vicia sativa L.