title: Some Operational Characteristics of Glycine Release in Rat Retina: The Role of Reverse Mode Operation of Glycine Transporter Type-1 (GlyT-1) in Ischemic Conditions.
abstract: Rat posterior eyecups containing the retina were prepared, loaded with [(3)H]glycine and superfused in order to determine its release originated from glycinergic amacrine cells and/or glial cells. Deprivation of oxygen and glucose from the Krebs-bicarbonate buffer used for superfusion evoked a marked increase of [(3)H]glycine release, an effect that was found to be external Ca(2+)-independent. Whereas oxygen and glucose deprivation increased [(3)H]glycine release, its uptake was reduced suggesting that energy deficiency shifts glycine transporter type-1 operation from normal to reverse mode. The increased release of [(3)H]glycine evoked by oxygen and glucose deprivation was suspended by addition of the non-competitive glycine transporter type-1 inhibitor NFPS and the competitive inhibitor ACPPB further suggesting the involvement of this transporter in the mediation of [(3)H]glycine release. Oxygen and glucose deprivation also evoked [(3)H]glutamate release from rat retina and the concomitantly occurring release of the NMDA receptor agonist glutamate and the coagonist glycine makes NMDA receptor pathological overstimulation possible in hypoxic conditions. [(3)H]Glutamate release was suspended by addition of the excitatory amino acid transporter inhibitor TBOA. Sarcosine, a substrate inhibitor of glycine transporter type-1, also increased [(3)H]glycine release probably by heteroexchange shifting transporter operation into reverse mode. This effect of sarcosine was also external Ca(2+)-independent and could be suspended by NFPS. Energy deficiency in retina induced by ouabain, an inhibitor of the Na(+)-K(+)-dependent ATPase, and by rotenone, a mitochondrial complex I inhibitor added with the glycolytic inhibitor 2-deoxy-D-glucose, led to increase of retinal [(3)H]glycine efflux. These effects of ouabain and rotenone/2-deoxy-D-glucose could also be blocked by NFPS pointed to the preferential reverse mode operation of glycine transporter type-1 as a consequence of impaired cellular energy homeostasis. Immunohistochemical studies revealed that glycine transporter type-1, of which reverse mode operation assures [(3)H]glycine release, is expressed in amacrine cells in the inner nuclear and plexiform layers of the retina and also in Müller macroglia cells. We conclude that disruption of the balanced normal/reverse mode operation of glycine transporter type-1 is likely a significant factor contributing to neurotoxic processes of the retina. The possibility to inhibit glycine transporter type-1 mediated glycine efflux by drugs more potently than glycine uptake might offer some therapeutic potential for the treatment of various neurodegenerative disorders of the retina.
authors.affiliation: Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvarad ter 4, Budapest, 1089, Hungary.
authors.forename: Adrienn
authors.initials: A
authors.lastname: Hanuska
authors.affiliation: Department of Pathophysiology, Semmelweis University, Budapest, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Szénási
authors.affiliation: CEVA Phylaxia Ltd, Budapest, Hungary.
authors.forename: Mihaly
authors.initials: M
authors.lastname: Albert
authors.affiliation: Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvarad ter 4, Budapest, 1089, Hungary.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Koles
authors.affiliation: Department of Applied Biotechnology and Food Science, Budapest University of Technology and Economics, Budapest, Hungary.
authors.forename: Agoston
authors.initials: A
authors.lastname: Varga
authors.affiliation: Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvarad ter 4, Budapest, 1089, Hungary.
authors.forename: Andras
authors.initials: A
authors.lastname: Szabo
authors.affiliation: Department of Organic Chemistry, Semmelweis University, Hogyes Endre u. 7, Budapest, 1092, Hungary. matyus.peter@pharma.semmelweis-univ.hu.
authors.forename: Peter
authors.initials: P
authors.lastname: Matyus
authors.affiliation: Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvarad ter 4, Budapest, 1089, Hungary. lharsing@t-email.hu.
authors.forename: Laszlo G
authors.initials: LG
authors.lastname: Harsing
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005998:Glycine / Q000378:metabolism*; D050487:Glycine Plasma Membrane Transport Proteins / Q000378:metabolism*; D007511:Ischemia / Q000378:metabolism*; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000187:drug effects / Q000378:metabolism*; D012171:Retinal Vessels / Q000378:metabolism*; D012521:Sarcosine / Q000494:pharmacology
chemical_list: D050487:Glycine Plasma Membrane Transport Proteins; C493266:Slc6a9 protein, rat; D010042:Ouabain; D005947:Glucose; D010100:Oxygen; D002118:Calcium; D005998:Glycine; D012521:Sarcosine
keywords: Glycine release; Glycine transporter; Glycine transporter inhibitors; Ischemia; Rat retina