title: Towards the Identification of Type III Effectors Associated with Ralstonia solanacearum Virulence on Tomato and Eggplant.
abstract: For the development of pathogen-informed breeding strategies, identifying the microbial genes involved in interactions with the plant is a critical step. To identify type III effector (T3E) repertoires associated with virulence of the bacterial wilt pathogen Ralstonia solanacearum on Solanaceous crops, we used an original association genetics approach combining DNA microarray data and pathogenicity data on resistant eggplant, pepper, and tomato accessions. From this first screen, 25 T3Es were further full-length polymerase chain reaction-amplified within a 35-strain field collection, to assess their distribution and allelic diversity. Six T3E repertoire groups were identified, within which 11 representative strains were chosen to challenge the bacterial wilt-resistant egg plants 'Dingras multiple Purple' and 'AG91-25', and tomato Hawaii 7996. The virulence or avirulence phenotypes could not be explained by specific T3E repertoires, but rather by individual T3E genes. We identified seven highly avirulence-associated genes, among which ripP2, primarily referenced as conferring avirulence to Arabidopsis thaliana. Interestingly, no T3E was associated with avirulence to both egg-plants. Highly virulence-associated genes were also identified: ripA5_2, ripU, and ripV2. This study should be regarded as a first step toward investigating both avirulence and virulence function of the highlighted genes, but also their evolutionary dynamics in natural R. solanacearum populations.
authors.affiliation: First, second, fourth, and sixth authors: CIRAD, UMR 53 Peuplements Végétaux et Bioagresseurs en Milieu Tropical (PVBMT), Saint-Pierre, La Réunion, France; third author: INRA, Centre d'Avignon, Unité de Génétique et Amélioration des Fruits et Légumes, UR1052, Montfavet, France; and fifth author: INRA, UMR 441 Laboratoire des Interactions Plantes-Microorganismes (LIPM), Castanet-Tolosan, France. Current address of first author: Institut National de la Recherche Agronomique, UMR A 1131 Santé de la Vigne et Qualité du Vin (SVQV), Colmar, France. Université de Strasbourg, Strasbourg, France.
authors.forename: Flora
authors.initials: F
authors.lastname: Pensec
authors.affiliation: First, second, fourth, and sixth authors: CIRAD, UMR 53 Peuplements Végétaux et Bioagresseurs en Milieu Tropical (PVBMT), Saint-Pierre, La Réunion, France; third author: INRA, Centre d'Avignon, Unité de Génétique et Amélioration des Fruits et Légumes, UR1052, Montfavet, France; and fifth author: INRA, UMR 441 Laboratoire des Interactions Plantes-Microorganismes (LIPM), Castanet-Tolosan, France. Current address of first author: Institut National de la Recherche Agronomique, UMR A 1131 Santé de la Vigne et Qualité du Vin (SVQV), Colmar, France. Université de Strasbourg, Strasbourg, France.
authors.forename: Aurore
authors.initials: A
authors.lastname: Lebeau
authors.affiliation: First, second, fourth, and sixth authors: CIRAD, UMR 53 Peuplements Végétaux et Bioagresseurs en Milieu Tropical (PVBMT), Saint-Pierre, La Réunion, France; third author: INRA, Centre d'Avignon, Unité de Génétique et Amélioration des Fruits et Légumes, UR1052, Montfavet, France; and fifth author: INRA, UMR 441 Laboratoire des Interactions Plantes-Microorganismes (LIPM), Castanet-Tolosan, France. Current address of first author: Institut National de la Recherche Agronomique, UMR A 1131 Santé de la Vigne et Qualité du Vin (SVQV), Colmar, France. Université de Strasbourg, Strasbourg, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Daunay
authors.affiliation: First, second, fourth, and sixth authors: CIRAD, UMR 53 Peuplements Végétaux et Bioagresseurs en Milieu Tropical (PVBMT), Saint-Pierre, La Réunion, France; third author: INRA, Centre d'Avignon, Unité de Génétique et Amélioration des Fruits et Légumes, UR1052, Montfavet, France; and fifth author: INRA, UMR 441 Laboratoire des Interactions Plantes-Microorganismes (LIPM), Castanet-Tolosan, France. Current address of first author: Institut National de la Recherche Agronomique, UMR A 1131 Santé de la Vigne et Qualité du Vin (SVQV), Colmar, France. Université de Strasbourg, Strasbourg, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Chiroleu
authors.affiliation: First, second, fourth, and sixth authors: CIRAD, UMR 53 Peuplements Végétaux et Bioagresseurs en Milieu Tropical (PVBMT), Saint-Pierre, La Réunion, France; third author: INRA, Centre d'Avignon, Unité de Génétique et Amélioration des Fruits et Légumes, UR1052, Montfavet, France; and fifth author: INRA, UMR 441 Laboratoire des Interactions Plantes-Microorganismes (LIPM), Castanet-Tolosan, France. Current address of first author: Institut National de la Recherche Agronomique, UMR A 1131 Santé de la Vigne et Qualité du Vin (SVQV), Colmar, France. Université de Strasbourg, Strasbourg, France.
authors.forename: Alice
authors.initials: A
authors.lastname: Guidot
authors.affiliation: First, second, fourth, and sixth authors: CIRAD, UMR 53 Peuplements Végétaux et Bioagresseurs en Milieu Tropical (PVBMT), Saint-Pierre, La Réunion, France; third author: INRA, Centre d'Avignon, Unité de Génétique et Amélioration des Fruits et Légumes, UR1052, Montfavet, France; and fifth author: INRA, UMR 441 Laboratoire des Interactions Plantes-Microorganismes (LIPM), Castanet-Tolosan, France. Current address of first author: Institut National de la Recherche Agronomique, UMR A 1131 Santé de la Vigne et Qualité du Vin (SVQV), Colmar, France. Université de Strasbourg, Strasbourg, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Wicker
mesh_terms: D055028:Comparative Genomic Hybridization; D018551:Solanum lycopersicum / Q000382:microbiology*; D010641:Phenotype; D043368:Ralstonia solanacearum / Q000235:genetics / Q000472:pathogenicity*; D032323:Solanum melongena / Q000382:microbiology*; D014774:Virulence