title: Construction of a DNA Library on Microbeads Using Whole Genome Amplification.
abstract: A polymerase chain reaction (PCR) in water droplets with water-in-oil emulsion (emulsion PCR) facilitates parallel amplification of a single-molecule template. The amplified DNA can be immobilized onto microbeads bound to primer DNA. The product, termed a "bead library", has various applications such as next-generation sequencing (NGS) and the directed evolution of various functional biomolecules. Here, we describe a method for genomic library construction on microbeads using emulsion PCR.
authors.affiliation: Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kojima
authors.affiliation: Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan. hnakano@agr.nagoya-u.ac.jp.
authors.forename: Hideo
authors.initials: H
authors.lastname: Nakano
mesh_terms: D004655:Emulsions; D016678:Genome; D015698:Genomic Library; D023281:Genomics / Q000379:methods*; D008863:Microspheres; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004655:Emulsions
keywords: Emulsion PCR; Genomic library; Magnetic beads; Microdroplets