title: Stimulation of Innate and Adaptive Immunity by Using Filamentous Bacteriophage fd Targeted to DEC-205.
abstract: The filamentous bacteriophage fd, codisplaying antigenic determinants and a single chain antibody fragment directed against the dendritic cell receptor DEC-205, is a promising vaccine candidate for its safety and its ability to elicit innate and adaptive immune response in absence of adjuvants. By using a system vaccinology approach based on RNA-Sequencing (RNA-Seq) analysis, we describe a relevant gene modulation in dendritic cells pulsed with anti-DEC-205 bacteriophages fd. RNA-Seq data analysis indicates that the bacteriophage fd virions are sensed as a pathogen by dendritic cells; they activate the danger receptors that trigger an innate immune response and thus confer a strong adjuvanticity that is needed to obtain a long-lasting adaptive immune response.
authors.affiliation: Institute of Protein Biochemistry (IBP), National Council of Research, 80131 Naples, Italy.
authors.forename: Luciana
authors.initials: L
authors.lastname: D'Apice
authors.affiliation: Institute of Genetics and Biophysics "A. Buzzati-Traverso" (IGB), National Council of Research, 80131 Naples, Italy.
authors.forename: Valerio
authors.initials: V
authors.lastname: Costa
authors.affiliation: Institute of Protein Biochemistry (IBP), National Council of Research, 80131 Naples, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Sartorius
authors.affiliation: Institute of Protein Biochemistry (IBP), National Council of Research, 80131 Naples, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Trovato
authors.affiliation: Institute of Genetics and Biophysics "A. Buzzati-Traverso" (IGB), National Council of Research, 80131 Naples, Italy.
authors.forename: Marianna
authors.initials: M
authors.lastname: Aprile
authors.affiliation: Institute of Protein Biochemistry (IBP), National Council of Research, 80131 Naples, Italy.
authors.forename: Piergiuseppe
authors.initials: P
authors.lastname: De Berardinis
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D017104:Bacteriophage M13 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D061505:Cell Surface Display Techniques; D016000:Cluster Analysis; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D007113:Immunity, Innate; D056747:Immunomodulation; D037181:Lectins, C-Type / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D015778:Minor Histocompatibility Antigens; D019151:Peptide Library; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D057127:Single-Chain Antibodies / Q000235:genetics / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D059467:Transcriptome
chemical_list: D015703:Antigens, CD; C093569:DEC-205 receptor; D018984:Epitopes, T-Lymphocyte; D037181:Lectins, C-Type; D015778:Minor Histocompatibility Antigens; D019151:Peptide Library; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D057127:Single-Chain Antibodies