title: Microbial ecology overview during anaerobic codigestion of dairy wastewater and cattle manure and use in agriculture of obtained bio-fertilisers.
abstract: The anaerobic co-digestion of dairy wastewater (DW) and cattle manure (CM) was examined and associated with microbial community's structures using Denaturing Gradient Gel Electrophoresis (DGGE). The highest volatile solids (VS) reduction yield of 88.6% and biogas production of 0.87 L/g VS removed were obtained for the C/N ratio of 24.7 at hydraulic retention time (HRT) of 20 days. The bacterial DGGE profile showed significant abundance of Uncultured Bacteroidetes, Firmicutes and Synergistetes bacterium. The Syntrophomonas strains were discovered in dependent association to H2-using bacteria such as Methanospirillum sp., Methanosphaera sp. and Methanobacterium formicicum. These syntrophic associations are essential in anaerobic digesters allow them to keep low hydrogen partial pressure. However, high concentrations of VFA produced from dairy wastes acidification allow the growth of Methanosarcina species. The application of the stabilised anaerobic effluent on the agriculture soil showed significant beneficial effects on the forage corn and tomato plants growth and crops.
authors.affiliation: UR-Biodiversity and Biology of Populations, FST, University of Elmanar, Tunis, Tunisia; LR-Microbial Ecology and Technology, INSAT, University of Carthage, Tunis, Tunisia.
authors.forename: Jihen
authors.initials: J
authors.lastname: Toumi
authors.affiliation: LR-Microbial Ecology and Technology, INSAT, University of Carthage, Tunis, Tunisia.
authors.forename: Baligh
authors.initials: B
authors.lastname: Miladi
authors.affiliation: LR-Microbial Ecology and Technology, INSAT, University of Carthage, Tunis, Tunisia.
authors.forename: Amel
authors.initials: A
authors.lastname: Farhat
authors.affiliation: UR-Biodiversity and Biology of Populations, FST, University of Elmanar, Tunis, Tunisia.
authors.forename: Said
authors.initials: S
authors.lastname: Nouira
authors.affiliation: LR-Microbial Ecology and Technology, INSAT, University of Carthage, Tunis, Tunisia.
authors.forename: Moktar
authors.initials: M
authors.lastname: Hamdi
authors.affiliation: Laboratoire Microorganismes et Biomolécules Actives, Université de Tunis El Manar, Tunisia.
authors.forename: Maher
authors.initials: M
authors.lastname: Gtari
authors.affiliation: LR-Microbial Ecology and Technology, INSAT, University of Carthage, Tunis, Tunisia. Electronic address: hassibbouallagui@yahoo.fr.
authors.forename: Hassib
authors.initials: H
authors.lastname: Bouallagui
mesh_terms: D000383:Agriculture; D000693:Anaerobiosis; D000818:Animals; D001421:Bacteria, Anaerobic / Q000235:genetics; D056804:Biofuels / Q000032:analysis; D019149:Bioreactors / Q000382:microbiology; D002417:Cattle; D003612:Dairying; D058645:Denaturing Gradient Gel Electrophoresis; D004063:Digestion / Q000502:physiology*; D005308:Fertilizers; D006859:Hydrogen / Q000032:analysis; D008372:Manure / Q000032:analysis*; D017013:Methanobacteriaceae; D017020:Methanosarcina; D062065:Wastewater / Q000032:analysis*; D003313:Zea mays
chemical_list: D056804:Biofuels; D005308:Fertilizers; D006859:Hydrogen; D008372:Manure; D062065:Wastewater
keywords: Agronomic application; Anaerobic digestion; Dairy wastewater; Manures; Microbial ecology