title: Effect of Sodium Selenite on Pathological Changes and Renal Functions in Broilers Fed a Diet Containing Aflatoxin B₁.
abstract: To evaluate the renal toxicity of dietary aflatoxin B₁ (AFB₁) and ameliorating effects of added dietary sodium selenite in broiler, renal histopathological changes, ultrastructural changes, and renal function parameters were monitored at 7, 14, and 21 days of age. Two hundred one-day-old healthy male Avian broilers were divided into four groups, namely control group, AFB₁ group (0.3 mg/kg AFB₁), +Se group (0.4 mg/kg Se), and AFB₁+Se group (0.3 mg/kg AFB₁+0.4 mg/kg Se). Compared with that of the control group, the relative weight of kidney was increased in the AFB₁ group. There were no significant differences between the AFB₁+Se group and the control group. By histopathological observation, the renal epithelia were swelling and necrosis at 7 and 21 days of age. Ultrastructurally, the lipid droplets and expanded endoplasmic reticulum appeared in the plasma of epithelia cells in the AFB₁ group. Enlarged mitochondria with degenerated cristae were observed in the +Se group. Compared with the control group, the contents of serum creatinine and serum uric acid in the AFB₁ group were increased, while the activity of renal Na⁺-K⁺ ATPase was decreased. When 0.4 mg/kg selenium was added into the diet containing 0.3 mg/kg AFB₁, there were no obvious histological changes in the AFB₁+Se group, and the contents of the serum creatinine and serum uric acid contents and the activity of renal Na⁺-K⁺ ATPase were close to those in the control group. In conclusion, sodium selenite exhibited protective effects on AFB₁-induced kidney toxicity in broilers.
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. liangna19911314@163.com.
authors.forename: Na
authors.initials: N
authors.lastname: Liang
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. wfy_sccd@163.com.
authors.forename: Fengyuan
authors.initials: F
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. pengxi197313@163.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. fangjing4109@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. cui580420@sicau.edu.cn.
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. chzhli75@163.com.
authors.forename: Zhengli
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. nwm_mm2004@163.com.
authors.forename: Weimin
authors.initials: W
authors.lastname: Lai
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. 13981616210@139.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. gengyisicau@126.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Geng
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D001835:Body Weight; D002645:Chickens; D003404:Creatinine / Q000097:blood; D019587:Dietary Supplements; D007668:Kidney / Q000187:drug effects* / Q000648:ultrastructure; D007674:Kidney Diseases / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D011042:Poisons / Q000633:toxicity*; D018038:Sodium Selenite / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014131:Trace Elements / Q000494:pharmacology*; D014527:Uric Acid / Q000097:blood
chemical_list: D011042:Poisons; D014131:Trace Elements; D014527:Uric Acid; D016604:Aflatoxin B1; D003404:Creatinine; D000254:Sodium-Potassium-Exchanging ATPase; D018038:Sodium Selenite
keywords: aflatoxin B1; broiler; pathological observation; renal function; sodium selenite; ultrastructural observation