title: Single immunization with a recombinant multiple-epitope protein induced protection against FMDV type Asia 1 in cattle.
abstract: To develop recombinant epitope vaccines against the foot-and-mouth disease virus (FMDV) serotype Asia 1, genes encoding six recombinant proteins (A1-A6) consisting of different combinations of B-cell and T-cell epitopes from VP1 capsid protein (VP1) of FMDV were constructed. These proteins were expressed in Escherichia coli and used to immunize animals. Our results showed that A6 elicited higher titers of neutralizing antibodies after single inoculation in guinea pigs than did the other five recombinant proteins, as determined by micro-neutralization tests. In addition, a strong lymphocyte proliferation response and Th1 type immunity were observed in splenocytes from the mice immunized with A6. Further tests carried out in cattle demonstrated that a single inoculation with A6 generated comparable levels of neutralizing antibodies as inactivated vaccine and protected 4 of 5 cattle against challenge with FMDV type Asia 1. Our results suggest that A6 might be a promising recombinant vaccine against FMDV type Asia 1 in cattle.
authors.affiliation: Department of Molecular Biology, Norman Bethune Medical College, Jilin University, Changchun 130021, China.
authors.forename: Mingli
authors.initials: M
authors.lastname: Fang
authors.affiliation: Department of Molecular Biology, Norman Bethune Medical College, Jilin University, Changchun 130021, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Tongyu
authors.initials: T
authors.lastname: Tang
authors.affiliation: Department of Molecular Biology, Norman Bethune Medical College, Jilin University, Changchun 130021, China.
authors.forename: Peiyan
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Immunology, Norman Bethune Medical College, Jilin University, Changchun 130021, China.
authors.forename: Junyang
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Molecular Biology, Norman Bethune Medical College, Jilin University, Changchun 130021, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Molecular Biology, Norman Bethune Medical College, Jilin University, Changchun 130021, China.
authors.forename: Hongfei
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Molecular Biology, Norman Bethune Medical College, Jilin University, Changchun 130021, China.
authors.forename: Haifei
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Molecular Biology, Norman Bethune Medical College, Jilin University, Changchun 130021, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wan
authors.affiliation: Inner Mongolia Jinyu Group Baoling Bio-pharmaceutical Corporation, Hohhot 013300,Inner Mongolia, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wei
authors.affiliation: Department of Immunology, Norman Bethune Medical College, Jilin University, Changchun 130021, China. Electronic address: ylyu@mail.jlu.edu.cn.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Molecular Biology, Norman Bethune Medical College, Jilin University, Changchun 130021, China. Electronic address: wlying@mail.jlu.edu.cn.
authors.forename: Liying
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D006168:Guinea Pigs; D007114:Immunization; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018417:Th1 Cells / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; C442501:VP1 protein, Foot-and-mouth disease virus; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic
keywords: Foot-and-mouth disease virus (FMDV); Recombinant-epitope vaccine; Serotype Asia 1; VP1 capsid protein