title: In search of key genes associated with endometriosis using bioinformatics approach.
abstract: OBJECTIVE
  The aim of this study was to identify key genes associated with endometriosis.
  
  
  METHODS
  Microarray data GSE7846 was downloaded from Gene Expression Omnibus. The differentially expressed genes (DEGs) in human endometrial endothelial cells derived from eutopic endometrium of patients with endometriosis compared with controls without endometriosis were identified using Linear Models for Microarray Data (LIMMA) package in R. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and gene ontology (GO) enrichment analyses were performed using DAVID. Moreover, the protein-protein interaction (PPI) network was constructed by STRING and subsequently significantly enriched modules were mined by ClusterONE. Finally, protein domains and KEGG pathway enrichment analyses were performed for PPI modules.
  
  
  RESULTS
  A total of 687 DEGs were identified, including 584 up- and 103 down-regulated genes. The up-regulated DEGs, such as epidermal growth factor (EGF) and interleukin 1 beta (IL-1β) were significantly enriched in KEGG pathways of focal adhesion, ECM-receptor interaction and calcium signaling pathway. Similarly, only one module was obtained form PPI network, and the genes, like angiotensin II receptor, Type 1 (AGTR1) in the module were mainly enriched in protein domain of rhodopsin-like G protein-coupled receptors and most altered pathways of neuroactive ligand-receptor interaction, calcium signaling pathway and vascular smooth muscle contraction.
  
  
  CONCLUSIONS
  Our findings indicate that EGF, IL-1β and AGTR1 may play important roles in the pathogenesis of endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, Shandong University Affiliated Jinan Center Hospital, Jinan, Shandong 250013, China.
authors.forename: Fangmei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Shandong University Affiliated Jinan Center Hospital, Jinan, Shandong 250013, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Lv
authors.affiliation: Department of Obstetrics and Gynecology, Shandong University Affiliated Jinan Center Hospital, Jinan, Shandong 250013, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Women and Children Health Care, Jinan Lixia District Public Health Bureau Maternity and Child Care Center, Jinan, Shandong 250011, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, Jinan Changqing District Ping'an Sub-district Community Service Center, Jinan, Shandong 250306, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of Obstetrics and Gynecology, Shandong University Affiliated Jinan Center Hospital, Jinan, Shandong 250013, China. Electronic address: kunzoukzkz@163.com.
authors.forename: Kun
authors.initials: K
authors.lastname: Zou
mesh_terms: D016022:Case-Control Studies; D019295:Computational Biology; D030541:Databases, Genetic; D015536:Down-Regulation; D004715:Endometriosis / Q000235:genetics*; D004815:Epidermal Growth Factor / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics; D054730:Protein Interaction Domains and Motifs / Q000235:genetics; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D015854:Up-Regulation
chemical_list: C544065:AGTR1 protein, human; D053583:Interleukin-1beta; D044140:Receptor, Angiotensin, Type 1; D004815:Epidermal Growth Factor
keywords: Differentially expressed genes; Endometriosis; Module; Protein–protein interaction network