title: Analysis of the Mycoplasma genitalium MgpB Adhesin to Predict Membrane Topology, Investigate Antibody Accessibility, Characterize Amino Acid Diversity, and Identify Functional and Immunogenic Epitopes.
abstract: Mycoplasma genitalium is a sexually transmitted pathogen and is associated with reproductive tract disease that can be chronic in nature despite the induction of a strong antibody response. Persistent infection exacerbates the likelihood of transmission, increases the risk of ascension to the upper tract, and suggests that M. genitalium may possess immune evasion mechanism(s). Antibodies from infected patients predominantly target the MgpB adhesin, which is encoded by a gene that recombines with homologous donor sequences, thereby generating sequence variation within and among strains. We have previously characterized mgpB heterogeneity over the course of persistent infection and have correlated the induction of variant-specific antibodies with the loss of that particular variant from the infected host. In the current study, we examined the membrane topology, antibody accessibility, distribution of amino acid diversity, and the location of functional and antigenic epitopes within the MgpB adhesin. Our results indicate that MgpB contains a single transmembrane domain, that the majority of the protein is surface exposed and antibody accessible, and that the attachment domain is located within the extracellular C-terminus. Not unexpectedly, amino acid diversity was concentrated within and around the three previously defined variable regions (B, EF, and G) of MgpB; while nonsynonymous mutations were twice as frequent as synonymous mutations in regions B and G, region EF had equal numbers of nonsynonymous and synonymous mutations. Interestingly, antibodies produced during persistent infection reacted predominantly with the conserved C-terminus and variable region B. In contrast, infection-induced antibodies reacted poorly with the N-terminus, variable regions EF and G, and intervening conserved regions despite the presence of predicted B cell epitopes. Overall, this study provides an important foundation to define how different segments of the MgpB adhesin contribute to functionality, variability, and immunogenicity during persistent M. genitalium infection.
authors.affiliation: Department of Medicine, Division of Infectious Diseases, University of Washington, Seattle, WA, United States of America.
authors.forename: Stefanie L
authors.initials: SL
authors.lastname: Iverson-Cabral
authors.affiliation: Department of Medicine, Division of Infectious Diseases, University of Washington, Seattle, WA, United States of America.
authors.forename: Gwendolyn E
authors.initials: GE
authors.lastname: Wood
authors.affiliation: Department of Medicine, Division of Infectious Diseases, University of Washington, Seattle, WA, United States of America; Department of Global Health, Pathobiology Interdisciplinary Program, University of Washington, Seattle, WA, United States of America.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Totten
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D000595:Amino Acid Sequence / Q000502:physiology; D000596:Amino Acids / Q000235:genetics / Q000276:immunology; D000818:Animals; D000937:Antigen-Antibody Reactions / Q000276:immunology; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000378:metabolism*; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D005260:Female; D008254:Macaca nemestrina; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000276:immunology; D009175:Mycoplasma Infections / Q000276:immunology; D045704:Mycoplasma genitalium / Q000276:immunology; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D018829:Adhesins, Bacterial; D000596:Amino Acids; D000939:Epitopes; C073268:adhesin, Mycoplasma genitalium