title: Splenic gene expression profiling in White Leghorn layer inoculated with the Salmonella enterica serovar Enteritidis.
abstract: Salmonella enterica serovar Enteritidis (SE) is a foodborne pathogen that can threaten human health through contaminated poultry products. Live poultry, chicken eggs and meat are primary sources of human salmonellosis. To understand the genetic resistance of egg-type chickens in response to SE inoculation, global gene expression in the spleen of 20-week-old White Leghorn was measured using the Agilent 4 × 44 K chicken microarray at 7 and 14 days following SE inoculation (dpi). Results showed that there were 1363 genes significantly differentially expressed between inoculated and non-inoculated groups at 7 dpi (I7/N7), of which 682 were up-regulated and 681 were down-regulated genes. By contrast, 688 differentially expressed genes were observed at 14 dpi (I14/N14), of which 371 were up-regulated genes and 317 were down-regulated genes. There were 33 and 28 immune-related genes significantly differentially expressed in the comparisons of I7/N7 and I14/N14 respectively. Functional annotation revealed that several Gene Ontology (GO) terms related to immunity were significantly enriched between the inoculated and non-inoculated groups at 14 dpi but not at 7 dpi, despite a similar number of immune-related genes identified between I7/N7 and I14/N14. The immune response to SE inoculation changes with different time points following SE inoculation. The complicated interaction between the immune system and metabolism contributes to the immune responses to SE inoculation of egg-type chickens at 14 dpi at the onset of lay. GC, TNFSF8, CD86, CD274, BLB1 and BLB2 play important roles in response to SE inoculation. The results from this study will deepen the current understanding of the genetic response of the egg-type chicken to SE inoculation at the onset of egg laying.
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271018, China.
authors.forename: Guixian
authors.initials: G
authors.lastname: Wu
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong, 271018, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271018, China.
authors.forename: Yukai
authors.initials: Y
authors.lastname: Qi
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271018, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
authors.forename: Guiyun
authors.initials: G
authors.lastname: Xu
authors.affiliation: Department of Animal Science, University of California, Davis, CA, 95616, USA.
authors.forename: Huaijun
authors.initials: H
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271018, China.|Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, Shandong, 271018, China.
authors.forename: Xianyao
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000382:microbiology; D060467:Disease Resistance / Q000235:genetics; D020869:Gene Expression Profiling; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000235:genetics* / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000235:genetics*; D012477:Salmonella enteritidis; D013154:Spleen / Q000378:metabolism*; D059467:Transcriptome
keywords: Salmonellosis; chicken; genome; microarray; regulation