title: Matrix Gla protein regulates differentiation of endothelial cells derived from mouse embryonic stem cells.
abstract: Matrix Gla protein (MGP) is an antagonist of bone morphogenetic proteins and expressed in vascular endothelial cells. Lack of MGP causes vascular abnormalities in multiple organs in mice. The objective of this study is to define the role of MGP in early endothelial differentiation. We find that expression of endothelial markers is highly induced in Mgp null organs, which, in wild type, contain high MGP expression. Furthermore, Mgp null embryonic stem cells express higher levels of endothelial markers than wild-type controls and an abnormal temporal pattern of expression. We also find that the Mgp-deficient endothelial cells adopt characteristics of mesenchymal stem cells. We conclude that loss of MGP causes dysregulation of early endothelial differentiation.
authors.affiliation: Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095-1679, USA.|Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 Western Yanta Road, Xi'an, 710061, China.
authors.forename: Jiayi
authors.initials: J
authors.lastname: Yao
authors.affiliation: Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095-1679, USA.
authors.forename: Pierre J
authors.initials: PJ
authors.lastname: Guihard
authors.affiliation: Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095-1679, USA.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Blazquez-Medela
authors.affiliation: Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095-1679, USA.
authors.forename: Yina
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095-1679, USA.|Shanghai Key Laboratory of Gynecologic Oncology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dong Fang Rd, Shanghai, 200127, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095-1679, USA.|The Molecular Biology Institute at UCLA, Los Angeles, CA, 90095-1570, USA.
authors.forename: Kristina I
authors.initials: KI
authors.lastname: Boström
authors.affiliation: Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095-1679, USA. yyao@mednet.ucla.edu.|Division of Cardiology, David Geffen School of Medicine at UCLA, Box 951679, Los Angeles, CA, 90095-1679, USA. yyao@mednet.ucla.edu.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Yao
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000172:deficiency / Q000378:metabolism*; D002452:Cell Count; D002454:Cell Differentiation; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000172:deficiency / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology*; D000097568:Matrix Gla Protein
chemical_list: D015415:Biomarkers; D002135:Calcium-Binding Proteins; D016326:Extracellular Matrix Proteins; D000097568:Matrix Gla Protein
keywords: Bone morphogenetic proteins; Differentiation; Embryonic stem cells; Endothelial cells; Matrix Gla protein