title: Nanopatterned Human iPSC-based Model of a Dystrophin-Null Cardiomyopathic Phenotype.
abstract: Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) offer unprecedented opportunities to study inherited heart conditionsin vitro, but are phenotypically immature, limiting their ability to effectively model adult-onset diseases. Cardiomyopathy is becoming the leading cause of death in patients with Duchenne muscular dystrophy (DMD), but the pathogenesis of this disease phenotype is not fully understood. Therefore, we aimed to test whether biomimetic nanotopography could further stratify the disease phenotype of DMD hiPSC-CMs to create more translationally relevant cardiomyocytes for disease modeling applications. We found that anisotropic nanotopography was necessary to distinguish structural differences between normal and DMD hiPSC-CMs, as these differences were masked on conventional flat substrates. DMD hiPSC-CMs exhibited a diminished structural and functional response to the underlying nanotopography compared to normal cardiomyocytes at both the macroscopic and subcellular levels. This blunted response may be due to a lower level of actin cytoskeleton turnover as measured by fluorescence recovery after photobleaching. Taken together these data suggest that DMD hiPSC-CMs are less adaptable to changes in their extracellular environment, and highlight the utility of nanotopographic substrates for effectively stratifying normal and structural cardiac disease phenotypesin vitro.
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, WA, USA ; Institute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA.
authors.forename: Jesse
authors.initials: J
authors.lastname: Macadangdang
authors.affiliation: Department of Physiology and Pharmacology, School of Medicine, Wake Forest University Health Sciences, Winston-Salem, NC, USA ; Department of Rehabilitation Medicine, University of Washington, Seattle, WA, USA ; Institute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA.
authors.forename: Xuan
authors.initials: X
authors.lastname: Guan
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, WA, USA.
authors.forename: Alec S T
authors.initials: AS
authors.lastname: Smith
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, WA, USA.
authors.forename: Rachel
authors.initials: R
authors.lastname: Lucero
authors.affiliation: Department of Rehabilitation Medicine, University of Washington, Seattle, WA, USA ; Institute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA.
authors.forename: Stefan
authors.initials: S
authors.lastname: Czerniecki
authors.affiliation: Department of Rehabilitation Medicine, University of Washington, Seattle, WA, USA ; Institute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA.
authors.forename: Martin K
authors.initials: MK
authors.lastname: Childers
authors.affiliation: Department of Rehabilitation Medicine, University of Washington, Seattle, WA, USA ; Institute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA.
authors.forename: David L
authors.initials: DL
authors.lastname: Mack
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, WA, USA ; Institute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA.
authors.forename: Deok-Ho
authors.initials: DH
authors.lastname: Kim
keywords: Cytoskeletal Alignment; Duchenne muscular dystrophy; FRAP; Stem Cell Derived Cardiomyocytes