title: A novel role of long non-coding RNAs in response to X-ray irradiation.
abstract: In the present study, the role of lncRNAs in response to radiation-induced DNA damage and oxidative stress were explored to improve our understanding of the biological pathways activated upon radiation-induced toxicity. The toxicity of X-ray radiation on human bronchial epithelial cell lines (HBE) was determined through a dose-dependent increase in ROS production and γ-H2AX formation and changes to lncRNA expression was observed and quantified using lncRNA-specific microarrays. 115 lncRNAs expression was increased in a dose-dependent manner following X-ray irradiation. Bioinformatic prediction algorithms determined that these lncRNAs significantly affect the p53 signaling pathway, and, more specifically, the BRCA 1 transcription factor and coding genes adjacent to BRCA 1. Our results highlight a previously uncharacterized role for lncRNAs to act via the p53-pathway in response to X-ray-induced DNA damage, and suggest lncRNAs may serve as novel indicators for radiation toxicity.
authors.affiliation: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, P. R. China; School of Public Health, Medical College of Soochow University, Suzhou 215123, P. R. China; Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Suzhou 215123, P. R. China.
authors.forename: Jihua
authors.initials: J
authors.lastname: Nie
authors.affiliation: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, P. R. China.
authors.forename: Chaojun
authors.initials: C
authors.lastname: Peng
authors.affiliation: School of Public Health, Medical College of Soochow University, Suzhou 215123, P. R. China; Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Suzhou 215123, P. R. China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Pei
authors.affiliation: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, P. R. China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, P. R. China.
authors.forename: Han
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Urology, the First Affiliated Hospital of Soochow University, Suzhou 215006, P. R. China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Qi
authors.affiliation: School of Public Health, Medical College of Soochow University, Suzhou 215123, P. R. China; Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Suzhou 215123, P. R. China. Electronic address: tongjian@suda.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Tong
authors.affiliation: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, P. R. China; Department of Radiation Genetics, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, P. R. China; Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions and School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Suzhou 215123,P. R. China. Electronic address: jiaoyang@suda.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiao
mesh_terms: D002478:Cells, Cultured; D004249:DNA Damage; D006657:Histones / Q000032:analysis; D006801:Humans; D062085:RNA, Long Noncoding / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000528:radiation effects; D016159:Tumor Suppressor Protein p53 / Q000502:physiology; D014965:X-Rays
chemical_list: C468783:H2AX protein, human; D006657:Histones; D062085:RNA, Long Noncoding; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53
keywords: BRCA 1; DNA damage; Long non-coding RNA (lncRNA); X-ray; p53