title: Bioorthogonal Enzymatic Activation of Caged Compounds.
abstract: Engineered cytochrome P450 monooxygenase variants are reported as highly active and selective catalysts for the bioorthogonal uncaging of propargylic and benzylic ether protected substrates, including uncaging in living E. coli. observed selectivity is supported by induced-fit docking and molecular dynamics simulations. This proof-of-principle study points towards the utility of bioorthogonal enzyme/protecting group pairs for applications in the life sciences.
authors.affiliation: Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Straße 4, 35043 Marburg (Germany).
authors.forename: Cornelia
authors.initials: C
authors.lastname: Ritter
authors.affiliation: Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Straße 4, 35043 Marburg (Germany).
authors.forename: Nathalie
authors.initials: N
authors.lastname: Nett
authors.affiliation: Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Straße 4, 35043 Marburg (Germany).|Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim an der Ruhr (Germany).|LOEWE Zentrum für Synthetische Mikrobiologie (SYNMIKRO), Hans-Meerwein-Straße, 35043 Marburg (Germany).|Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Straße 10, 35043 Marburg (Germany).
authors.forename: Carlos G
authors.initials: CG
authors.lastname: Acevedo-Rocha
authors.affiliation: Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Straße 4, 35043 Marburg (Germany).|Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim an der Ruhr (Germany).
authors.forename: Richard
authors.initials: R
authors.lastname: Lonsdale
authors.affiliation: Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Straße 4, 35043 Marburg (Germany).
authors.forename: Katja
authors.initials: K
authors.lastname: Kräling
authors.affiliation: LOEWE Zentrum für Synthetische Mikrobiologie (SYNMIKRO), Hans-Meerwein-Straße, 35043 Marburg (Germany).
authors.forename: Felix
authors.initials: F
authors.lastname: Dempwolff
authors.affiliation: Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Straße 4, 35043 Marburg (Germany).
authors.forename: Sabrina
authors.initials: S
authors.lastname: Hoebenreich
authors.affiliation: Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Straße 4, 35043 Marburg (Germany).|LOEWE Zentrum für Synthetische Mikrobiologie (SYNMIKRO), Hans-Meerwein-Straße, 35043 Marburg (Germany).
authors.forename: Peter L
authors.initials: PL
authors.lastname: Graumann
authors.affiliation: Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Straße 4, 35043 Marburg (Germany). reetz@mpi-muelheim.mpg.de.|Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim an der Ruhr (Germany). reetz@mpi-muelheim.mpg.de.
authors.forename: Manfred T
authors.initials: MT
authors.lastname: Reetz
authors.affiliation: Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Straße 4, 35043 Marburg (Germany). meggers@chemie.uni-marburg.de.|College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (P. R. China). meggers@chemie.uni-marburg.de.
authors.forename: Eric
authors.initials: E
authors.lastname: Meggers
mesh_terms: D000438:Alcohols / Q000737:chemistry / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism*; D004789:Enzyme Activation; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism*; D004987:Ethers / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D015202:Protein Engineering
chemical_list: D000438:Alcohols; D004987:Ethers; D003577:Cytochrome P-450 Enzyme System
keywords: bioorthogonal chemistry; cytochromes; directed evolution; protecting groups; protein engineering