title: Protective role of polyphenols from Bauhinia hookeri against carbon tetrachloride-induced hepato- and nephrotoxicity in mice.
abstract: The hepatoprotective and nephroprotective activity of a polyphenol-rich fraction (BHPF) obtained from Bauhinia hookeri was investigated against CCl4-induced acute hepatorenal toxicity in mice. BHPF was administered (100, 200 and 400 mg/kg/day) for 5 days, then CCl4 was administered. BHPF pretreatment significantly (p < 0.001) inhibited the CCl4-induced increase in ALT, AST, ALP, LDH, total bilirubin, cholesterol, creatinine, uric acid, urea and malondialdehyde in a dose-dependent manner. In contrast, BHPF pretreatment markedly increased the contents of glutathione and superoxide dismutase in the liver and kidney tissues, indicating the strong in vivo antioxidant activity of BHPF. Pretreatment with BHPF preserved the hepatic architecture and conferred marked protection against necrosis and ballooning degeneration. Pretreatment with BHPF reduced the inflammatory cell aggregation and degenerative changes in the lining epithelium of the kidney tubules. It can be concluded that BHPF has a remarkable hepato- and nephroprotective activity by enhancing the antioxidant defense status, reducing lipid peroxidation and protecting against the histopathological changes induced by CCl4 in the liver and kidney tissues.
authors.affiliation: a Department of Pharmacognosy, Faculty of Pharmacy , Ain-Shams University , Cairo , Egypt .
authors.forename: Eman
authors.initials: E
authors.lastname: Al-Sayed
authors.affiliation: b Department of Pharmacoloy, Faculty of Veterinary Medicine , Suez Canal University , Ismailia , Egypt .
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Abdel-Daim
authors.affiliation: c Department of Clinical Pathology, Faculty of Veterinary Medicine , Suez Canal University , Ismailia , Egypt , and.
authors.forename: Omnia E
authors.initials: OE
authors.lastname: Kilany
authors.affiliation: d Laboratory of Organic Chemistry and Chemical Biology, Department of Chemistry, University of Turku , Turku , Finland.
authors.forename: Maarit
authors.initials: M
authors.lastname: Karonen
authors.affiliation: d Laboratory of Organic Chemistry and Chemical Biology, Department of Chemistry, University of Turku , Turku , Finland.
authors.forename: Jari
authors.initials: J
authors.lastname: Sinkkonen
mesh_terms: D000818:Animals; D036481:Bauhinia; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology* / Q000517:prevention & control; D002851:Chromatography, High Pressure Liquid; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000473:pathology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000008:administration & dosage*; D020011:Protective Agents / Q000008:administration & dosage*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D059808:Polyphenols; D020011:Protective Agents; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Antioxidant; HPLC-PDA-ESI/MS/MS; epicatechin gallate; hepatoprotective; kidney; liver; nephroprotective; proanthocyanidins