title: Impact of hydroquinone used as a redox effector model on potential denitrification, microbial activity and redox condition of a cultivable soil.
abstract: In this microcosm study, we analyzed the effect produced by hydroquinone on the expression of soil biological denitrification, in relation to the redox state of the soil, both in terms of intensity factor (Eh') and capacity factor (amount of oxidized or reduced compounds). The supplementation of an Argiudoll soil with hydroquinone decreased the soil apparent reduction potential (Eh') and soil dehydrogenase activity (formazan production from tetrazolium chloride reduction; redox capacity factor), the relationship between both factors being highly significative, r=0.99 (p<0.001). The bacterial population (measured by colony forming units) increased, and the production of N2O was greater (p<0.001) at 200 and 400μg/g dry soil doses. Furthermore, there was an inverse relationship between soil dehydrogenase activity and the number of bacteria (r=-0.82; p<0.05), increased denitrification activity and changes in the CO2/N2O ratio value. These results suggest that hydroquinone at supplemented doses modified the soil redox state and the functional structure of the microbial population. Acetate supplementation on soil with hydroquinone, to ensure the availability of an energy source for microbial development, confirmed the tendency of the results obtained with the supplementation of hydroquinone alone. The differences observed at increased doses of hydroquinone might be explained by differences on the hydroquinone redox species between treatments.
authors.affiliation: Laboratorio de Química Biológica I, Facultad de Ciencias Veterinarias, Consejo de Investigaciones, Universidad Nacional de Rosario, Ov. Lagos y Ruta 33, 2170 Casilda, Provincia de Santa Fe, Argentina. Electronic address: eperotti@unr.edu.ar.
authors.forename: Elda B R
authors.initials: EB
authors.lastname: Perotti
mesh_terms: D000085:Acetates / Q000494:pharmacology; D001118:Argentina; D001426:Bacterial Proteins / Q000032:analysis; D058440:Denitrification / Q000187:drug effects*; D005656:Fungal Proteins / Q000032:analysis; D006873:Hydroquinones / Q000494:pharmacology*; D064307:Microbiota / Q000187:drug effects*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000032:analysis; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D005656:Fungal Proteins; D006873:Hydroquinones; D012987:Soil; D010088:Oxidoreductases; C031927:hydroquinone
keywords: Actividad deshidrogenasa; Condiciones redox; Dehydrogenase activity; Dosis de hidroquinona; Heterotrophic microorganisms; Hydroquinone doses; Microorganismos heterótrofos; Nitrous oxide; Redox conditions; Óxido nitroso