title: Protective effect of resveratrol against methotrexate-induced oxidative stress in the small intestinal tissues of rats.
abstract: The effect of resveratrol on the damage induced by methotrexate (MTX) in rat duodenum and jejunum tissue was investigated and evaluated in comparison with famotidine. The rats were divided into four groups as healthy group (HG), resveratrol+MTX (RMTX) group, famotidine+MTX (FMTX) group and the control group which received MTX (MTXC). RMTX group was given resveratrol 25 mg/kg and FMTX group famotidin 25 mg/kg, while MTXC and HG groups were orally administered distilled water once a day for 30 days. The rats in RMTX, FMTX and MTXC groups were given MTX of 5 mg/kg dose by the same way for 30 days. At the end of this period, amount of MDA, 8-OH/Gua and tGSH, and MPO gene expression were measured in the duodenal and jejunal tissues and the results were histopathologically evaluated. Resveratrol and famotidine were found to significantly prevent elevation of the MDA, 8-OH/Gua and MPO parameters with MTX and decrease of the levels of tGSH in the duodenal and jejunal tissues. Both drugs prevented severe damage to the villus and crypt epithelium in the duodenum and jejunum, congestion and hemorrhage, inflammatory cell infiltration and necrosis in the mucosa and submucosa due to MTX administration. Resveratrol could be considered in the clinical practice for treatment of the tissue damage in the intestines due to use of MTX, in comparison with famotidine. Resveratrol may be more advantageous than famotidine in long-term use against MTX toxicity since it does not inhibit gastric acid secretion.
authors.affiliation: Department of Internal Medicine, Istinye State Hospital Istanbul 34465, Turkey.
authors.forename: Aynur
authors.initials: A
authors.lastname: Arslan
authors.affiliation: Department of Internal Medicine, Faculty of Medicine, Erzincan University Erzincan 24030, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Ozcicek
authors.affiliation: Department of Pathology, Mengucek Gazi Training and Research Hospital Erzincan 24030, Turkey.
authors.forename: Ferda
authors.initials: F
authors.lastname: Keskin Cimen
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Erzincan University Erzincan 24030, Turkey.
authors.forename: Durdu
authors.initials: D
authors.lastname: Altuner
authors.affiliation: Department of Medical Genetic, Goztepe Training and Research Hospital, Istanbul Medeniyet University Istanbul 34732, Turkey.
authors.forename: Oguzhan
authors.initials: O
authors.lastname: Yarali
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Ataturk University Erzurum 25240, Turkey.
authors.forename: Nezahat
authors.initials: N
authors.lastname: Kurt
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Recep Tayyip Erdogan University Rize 53020, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Tumkaya
authors.affiliation: Department of Anatomy, Erzurum Region Education and Research Hospital Erzurum 25240, Turkey.
authors.forename: Cengiz
authors.initials: C
authors.lastname: Ozturk
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Erzincan University Erzincan 24030, Turkey.
authors.forename: Halis
authors.initials: H
authors.lastname: Suleyman
keywords: Resveratrol; oxidative stress; small intestine