title: Diterpenes from the roots of Oryza sativa L. and their inhibition activity on NO production in LPS-stimulated RAW264.7 macrophages.
abstract: Two new pimarane diterpenoids, momilactone D (3) and momilactone E (5), along with three known diterpenoids, momilactone A (1), sandaracopimaradien-3-one (2), and oryzalexin A (4) were isolated from Oryza sativa roots. The chemical structures of the compounds were determined by spectroscopic data analysis. The isolated diterpenoids were evaluated for their ability to inhibit NO production and iNOS mRNA and protein expression in LPS-stimulated RAW264.7 macrophages. Compound 4 showed strong inhibition activity on NO production, and compounds 1 and 4 decreased the expression of iNOS mRNA and protein levels.
authors.affiliation: Graduate School of Biotechnology and Research Institute of Life Sciences & Resources, Kyung Hee University, Yongin 446-701, Republic of Korea, (phone: +82-31-201-2610; fax: +82-31-204-8116).
authors.forename: Jin-Gyeong
authors.initials: JG
authors.lastname: Cho
authors.affiliation: Graduate School of Biotechnology and Research Institute of Life Sciences & Resources, Kyung Hee University, Yongin 446-701, Republic of Korea, (phone: +82-31-201-2610; fax: +82-31-204-8116).
authors.forename: Byeong-Ju
authors.initials: BJ
authors.lastname: Cha
authors.affiliation: Graduate School of Biotechnology and Research Institute of Life Sciences & Resources, Kyung Hee University, Yongin 446-701, Republic of Korea, (phone: +82-31-201-2610; fax: +82-31-204-8116).
authors.forename: Sang
authors.initials: S
authors.lastname: Min Lee
authors.affiliation: Graduate School of Biotechnology and Research Institute of Life Sciences & Resources, Kyung Hee University, Yongin 446-701, Republic of Korea, (phone: +82-31-201-2610; fax: +82-31-204-8116).
authors.forename: Sabina
authors.initials: S
authors.lastname: Shrestha
authors.affiliation: Graduate School of Biotechnology and Research Institute of Life Sciences & Resources, Kyung Hee University, Yongin 446-701, Republic of Korea, (phone: +82-31-201-2610; fax: +82-31-204-8116).
authors.forename: Rak-Hun
authors.initials: RH
authors.lastname: Jeong
authors.affiliation: Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Won Kwang University, Iksan 570-749, Republic of Korea.
authors.forename: Dong
authors.initials: D
authors.lastname: Sung Lee
authors.affiliation: Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Won Kwang University, Iksan 570-749, Republic of Korea.
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
authors.affiliation: R&D Center, GFC Co., Ltd., Suwon 443-813, Republic of Korea.
authors.forename: Dong-Geol
authors.initials: DG
authors.lastname: Lee
authors.affiliation: R&D Center, GFC Co., Ltd., Suwon 443-813, Republic of Korea.
authors.forename: Hee-Cheol
authors.initials: HC
authors.lastname: Kang
authors.affiliation: Graduate School of Biotechnology and Research Institute of Life Sciences & Resources, Kyung Hee University, Yongin 446-701, Republic of Korea, (phone: +82-31-201-2610; fax: +82-31-204-8116).
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Kim
authors.affiliation: Graduate School of Biotechnology and Research Institute of Life Sciences & Resources, Kyung Hee University, Yongin 446-701, Republic of Korea, (phone: +82-31-201-2610; fax: +82-31-204-8116). nibaek@khu.ac.kr.
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
mesh_terms: D000818:Animals; D002460:Cell Line; D004224:Diterpenes / Q000737:chemistry* / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000276:immunology*; D012275:Oryza / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D004224:Diterpenes; D008070:Lipopolysaccharides; D009569:Nitric Oxide
keywords: Momilactone D; Momilactone E; NO Production; RAW264.7; Rice roots; iNOS