title: Copy Number Variation Analysis by Array Analysis of Single Cells Following Whole Genome Amplification.
abstract: Whole genome amplification is required to ensure the availability of sufficient material for copy number variation analysis of a genome deriving from an individual cell. Here, we describe the protocols we use for copy number variation analysis of non-fixed single cells by array-based approaches following single-cell isolation and whole genome amplification. We are focusing on two alternative protocols, an isothermal and a PCR-based whole genome amplification method, followed by either comparative genome hybridization (aCGH) or SNP array analysis, respectively.
authors.affiliation: Center for Human Genetics, Laboratory for Cytogenetics and Genome Research, KU Leuven, Leuven, 3000, Belgium.
authors.forename: Eftychia
authors.initials: E
authors.lastname: Dimitriadou
authors.affiliation: Center for Human Genetics, Laboratory of Reproductive Genomics, KU Leuven, Leuven, 3000, Belgium.
authors.forename: Masoud
authors.initials: M
authors.lastname: Zamani Esteki
authors.affiliation: Center for Human Genetics, Laboratory for Cytogenetics and Genome Research, KU Leuven, Leuven, 3000, Belgium. Joris.vermeesch@uzleuven.be.
authors.forename: Joris Robert
authors.initials: JR
authors.lastname: Vermeesch
mesh_terms: D055028:Comparative Genomic Hybridization / Q000379:methods*; D056915:DNA Copy Number Variations; D016678:Genome; D023281:Genomics / Q000379:methods; D021141:Nucleic Acid Amplification Techniques; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D059010:Single-Cell Analysis / Q000379:methods*
keywords: Copy number variation analysis; Genomic microarrays; Multiple displacement amplification; Picoplex; Whole genome amplification