title: Analysis of colorectal cancer glyco-secretome identifies laminin β-1 (LAMB1) as a potential serological biomarker for colorectal cancer.
abstract: The high mortality rate in colorectal cancer is mostly ascribed to metastasis, but the only clinical biomarker available for disease monitoring and prognosis is the carcinoembryonic antigen (CEA). However, the prognostic utility of CEA remains controversial. In an effort to identify novel biomarkers that could be potentially translated for clinical use, we collected the secretomes from the colon adenocarcinoma cell line HCT-116 and its metastatic derivative, E1, using the hollow fiber culture system, and utilized the multilectin affinity chromatography approach to enrich for the secreted glycoproteins (glyco-secretome). The HCT-116 and E1 glyco-secretomes were compared using the label-free quantitative SWATH-MS technology, and a total of 149 glycoproteins were differentially secreted in E1 cells. Among these glycoproteins, laminin β-1 (LAMB1), a glycoprotein not previously known to be secreted in colorectal cancer cells, was observed to be oversecreted in E1 cells. In addition, we showed that LAMB1 levels were significantly higher in colorectal cancer patient serum samples as compared to healthy controls when measured using ELISA. ROC analyses indicated that LAMB1 performed better than CEA at discriminating between colorectal cancer patients from controls. Moreover, the diagnostic performance was further improved when LAMB1 was used in combination with CEA.
authors.affiliation: Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore.
authors.forename: Hannah S R
authors.initials: HS
authors.lastname: Lim
authors.affiliation: Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore.
authors.forename: Hui Ling
authors.initials: HL
authors.lastname: Lin
authors.affiliation: Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Hwee Tong
authors.initials: HT
authors.lastname: Tan
authors.affiliation: Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore.
authors.forename: Teck Kwang
authors.initials: TK
authors.lastname: Lim
authors.affiliation: Division of Colorectal Surgery, National University Hospital, Singapore.|Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Wai Kit
authors.initials: WK
authors.lastname: Cheong
authors.affiliation: Department of Colorectal Surgery, Singapore General Hospital, Singapore.|Saw Swee Hock School of Public Health, National University of Singapore, Singapore.|Duke-NUS Graduate Medical School, National University of Singapore, Singapore.
authors.forename: Peh Yean
authors.initials: PY
authors.lastname: Cheah
authors.affiliation: Department of Colorectal Surgery, Singapore General Hospital, Singapore.
authors.forename: Choong Leong
authors.initials: CL
authors.lastname: Tang
authors.affiliation: Department of General Surgery, Singapore General Hospital, Singapore.|Department of Surgical Oncology, National Cancer Centre, Singapore.|Centre for Quantitative Medicine, Duke-NUS Graduate Medical School, National University of Singapore, Singapore.
authors.forename: Pierce K H
authors.initials: PK
authors.lastname: Chow
authors.affiliation: Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.|Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore.
authors.forename: Maxey C M
authors.initials: MC
authors.lastname: Chung
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood* / Q000378:metabolism; D002272:Carcinoembryonic Antigen / Q000097:blood; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D006801:Humans; D007797:Laminin / Q000097:blood* / Q000378:metabolism; D009362:Neoplasm Metastasis; D020543:Proteome / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; C478221:LAMB1 protein, human; D007797:Laminin; D020543:Proteome
keywords: Biomedicine; Colorectal cancer; Glyco-secretome; LAMB1; MLAC; SWATH-MS