title: Autologous Infant and Allogeneic Adult Red Cells Demonstrate Similar Concurrent Post-Transfusion Survival in Very Low Birth Weight Neonates.
abstract: OBJECTIVE
  Based on the hypothesis that neonatal autologous red blood cell (RBC) survival (RCS) is substantially shorter than adult RBC, we concurrently tracked the survival of transfused biotin-labeled autologous neonatal and allogeneic adult RBC into ventilated, very low birth weight infants.
  
  
  METHODS
  RBC aliquots from the first clinically ordered, allogeneic adult RBC transfusion and from autologous infant blood were labeled at separate biotin densities (biotin-labeled RBC [BioRBC]) and transfused. Survival of these BioRBCs populations were concurrently followed over weeks by flow cytometric enumeration using leftover blood. Relative tracking of infant autologous and adult allogeneic BioRBC was analyzed by linear mixed modeling of batched weekly data. When possible, Kidd antigen (Jka and Jkb) mismatches between infant and donor RBCs were also used to track these 2 populations.
  
  
  RESULTS
  Contrary to our hypothesis, concurrent tracking curves of RCS of neonatal and adult BioRBC in 15 study infants did not differ until week 7, after which neonatal RCS became shortened to 59%-79% of adult enumeration values for uncertain reasons. Analysis of mismatched Kidd antigen RBC showed similar results, thus, confirming that BioRBC tracking is not perturbed by biotin RBC labeling.
  
  
  CONCLUSIONS
  This study illustrates the utility of multidensity BioRBC labeling for concurrent measurement of RCS of multiple RBC populations in vivo. The similar RCS results observed for neonatal and adult BioRBCs transfused into very low birth weight infants provides strong evidence that the circulatory environment of the newborn infant, not intrinsic infant-adult RBC differences, is the primary determinant of erythrocyte survival.
  
  
  BACKGROUND
  Clinicaltrials.gov: NCT00731588.
authors.affiliation: Department of Pediatrics, The University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA. Electronic address: john-widness@uiowa.edu.
authors.forename: John A
authors.initials: JA
authors.lastname: Widness
authors.affiliation: Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa College of Pharmacy, Iowa City, IA.
authors.forename: Denison J
authors.initials: DJ
authors.lastname: Kuruvilla
authors.affiliation: Department of Biochemistry and Molecular Biology and the Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR.
authors.forename: Donald M
authors.initials: DM
authors.lastname: Mock
authors.affiliation: Department of Biochemistry and Molecular Biology and the Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR.
authors.forename: Nell I
authors.initials: NI
authors.lastname: Matthews
authors.affiliation: Department of Pediatrics, The University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA.
authors.forename: Demet
authors.initials: D
authors.lastname: Nalbant
authors.affiliation: Department of Pediatrics, The University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA.
authors.forename: Gretchen A
authors.initials: GA
authors.lastname: Cress
authors.affiliation: Department of Pediatrics, The University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Schmidt
authors.affiliation: Department of Pediatrics, The University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA; Department of Pathology, The University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA.
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Strauss
authors.affiliation: Department of Biostatistics, University of Iowa College of Public Health, Iowa City, IA.
authors.forename: M Bridget
authors.initials: MB
authors.lastname: Zimmerman
authors.affiliation: Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa College of Pharmacy, Iowa City, IA.
authors.forename: Peter
authors.initials: P
authors.lastname: Veng-Pedersen
mesh_terms: D000328:Adult; D001710:Biotin / Q000737:chemistry*; D019921:Biotinylation; D002470:Cell Survival; D017707:Erythrocyte Transfusion / Q000379:methods*; D004920:Erythropoiesis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D019102:Infant, Very Low Birth Weight; D007667:Kidd Blood-Group System; D008297:Male; D008962:Models, Theoretical; D011446:Prospective Studies; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
chemical_list: D007667:Kidd Blood-Group System; D001710:Biotin