title: Systematic gene deletion and functional characterization of histidine kinase phosphorelay receptors (HKRs) in the human pathogenic fungus Aspergillus fumigatus.
abstract: Histidine kinase receptors (HKRs) appear to be a common strategy for model and pathogenic fungi to sense and respond to environmental stresses. In the human pathogen Aspergillus fumigatus, which is responsible for invasive aspergillosis, 13 genes potentially encoding HKRs have been identified. Until now, only three HKRs have been functionally characterized. The aim of this study was to perform the systematic invalidation of A. fumigatus HKR genes and the careful phenotypic characterization of the relevant mutants. This study notably allowed to gain new important insights into the role of HKRs in physiology of A. fumigatus. Actually, we showed that (i) NikA/TcsC could be involved in the cell wall integrity pathway, (ii) Fhk6 and PhkA were involved in the regulation of the "fluffy" developmental program, (iii) PhkB could participate in the regulation of conidiation and (iv) PhkA was implied in the resistance of oxidative stresses.
authors.affiliation: Univ Paris Descartes, Sorbonne Paris Cité, Institut des Energies de Demain (IED), UMR 8236, F-75205 Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Chapeland-Leclerc
authors.affiliation: Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 51664, Iran(1).
authors.forename: A
authors.initials: A
authors.lastname: Dilmaghani
authors.affiliation: AS International Group, 120-122 rue Réaumur, F-75002 Paris, France(1).
authors.forename: L
authors.initials: L
authors.lastname: Ez-Zaki
authors.affiliation: Génétique Quantitative et Évolution - Le Moulon, INRA - Université Paris-Sud - CNRS - AgroParisTech, Batiment 400, UFR des Sciences, F-91405 Orsay Cedex, France(1).
authors.forename: S
authors.initials: S
authors.lastname: Boisnard
authors.affiliation: CNRS, UPMC, UMR7622, F-75252 Paris Cedex 05, France(1).
authors.forename: B
authors.initials: B
authors.lastname: Da Silva
authors.affiliation: Laboratoire de Pharmacognosie, UMR CNRS Comète 8638, Faculté de Pharmacie, Université Paris Descartes, 4 Avenue de l'Observatoire, F-75006 Paris, France.
authors.forename: T
authors.initials: T
authors.lastname: Gaslonde
authors.affiliation: Laboratoire de Pharmacognosie, UMR CNRS Comète 8638, Faculté de Pharmacie, Université Paris Descartes, 4 Avenue de l'Observatoire, F-75006 Paris, France.
authors.forename: F H
authors.initials: FH
authors.lastname: Porée
authors.affiliation: Univ Paris Descartes, Sorbonne Paris Cité, Institut des Energies de Demain (IED), UMR 8236, F-75205 Paris, France. Electronic address: gwenael.ruprich-robert@parisdescartes.fr.
authors.forename: G
authors.initials: G
authors.lastname: Ruprich-Robert
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D001228:Aspergillosis / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D025141:Drug Resistance, Fungal; D005204:Farnesol / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics*; D017353:Gene Deletion; D000071677:Histidine Kinase; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D018384:Oxidative Stress / Q000187:drug effects; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D005204:Farnesol; D011494:Protein Kinases; D000071677:Histidine Kinase
keywords: Aspergillus fumigatus; Histidine kinase receptor; Stress response; Two-component pathway