title: 1H, 15N and 13C backbone assignments of GDP-bound human H-Ras mutant G12V.
abstract: Harvey Ras (H-Ras) is a membrane-associated GTPase with critical functions in cell proliferation and differentiation. The G12V mutant of H-Ras is one of the most commonly encountered oncoproteins in human cancer. This mutation disrupts the GTPase activity of H-Ras, leading to constitutive activation and aberrant downstream signalling. Here we report the backbone resonance assignments of human H-Ras mutant G12V lacking the C-terminal membrane attachment domain.
authors.affiliation: Astex Pharmaceuticals, 436 Cambridge Science Park, Cambridge, CB4 0QA, UK.|Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
authors.forename: Nader
authors.initials: N
authors.lastname: Amin
authors.affiliation: Astex Pharmaceuticals, 436 Cambridge Science Park, Cambridge, CB4 0QA, UK.|AstraZeneca, 310 Cambridge Science Park, Cambridge, CB4 0FZ, UK.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Chiarparin
authors.affiliation: Astex Pharmaceuticals, 436 Cambridge Science Park, Cambridge, CB4 0QA, UK.
authors.forename: Joe
authors.initials: J
authors.lastname: Coyle
authors.affiliation: Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge, CB2 1GA, UK.
authors.forename: Daniel
authors.initials: D
authors.lastname: Nietlispach
authors.affiliation: Astex Pharmaceuticals, 436 Cambridge Science Park, Cambridge, CB4 0QA, UK. glyn.williams@astx.com.
authors.forename: Glyn
authors.initials: G
authors.lastname: Williams
mesh_terms: D006153:Guanosine Diphosphate / Q000378:metabolism*; D006801:Humans; D050505:Mutant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D016283:Proto-Oncogene Proteins p21(ras) / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D050505:Mutant Proteins; D006153:Guanosine Diphosphate; D016283:Proto-Oncogene Proteins p21(ras)
keywords: Cancer; H-Ras; H-Ras G12V; NMR; NMR resonance assignments; Ras