title: Study of protein and RNA in dendritic spines using multi-isotope imaging mass spectrometry (MIMS).
abstract: The classical view of neuronal protein synthesis is that proteins are made in the cell body and then transported to their functional sites in the dendrites and the dendritic spines. Indirect evidence, however, suggests that protein synthesis can directly occur in the distal dendrites, far from the cell body. We are developing protocols for dual labeling of RNA and proteins using15N-uridine and18O- or13C-leucine pulse chase in cultured neurons to identify and localize both protein synthesis and fate of newly synthesized proteins. Pilot experiments show discrete localization of both RNA and newly synthesized proteins in dendrites, close to dendritic spines. We have for the first time directly imaged and measured the production of proteins at the subcellular level in the neuronal dendrites, close to the functional sites, the dendritic spines. This will open a powerful way to study neural growth and synapse plasticity in health and disease.
authors.affiliation: Science for Life Laboratory, Department of Woman and Child Health, Karolinska Institute, Astrid Lindgren Children's Hospital, Stockholm, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Brismar
authors.affiliation: Science for Life Laboratory, Department of Woman and Child Health, Karolinska Institute, Astrid Lindgren Children's Hospital, Stockholm, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Aperia
authors.affiliation: Science for Life Laboratory, Department of Woman and Child Health, Karolinska Institute, Astrid Lindgren Children's Hospital, Stockholm, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Westin
authors.affiliation: National Resource for Imaging Mass Spectrometry, Cambridge, MA USA.
authors.forename: J
authors.initials: J
authors.lastname: Moy
authors.affiliation: National Resource for Imaging Mass Spectrometry, Cambridge, MA USA.
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.affiliation: Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA USA ; National Resource for Imaging Mass Spectrometry, Cambridge, MA USA.
authors.forename: C
authors.initials: C
authors.lastname: Guillermier
authors.affiliation: National Resource for Imaging Mass Spectrometry, Cambridge, MA USA.
authors.forename: C
authors.initials: C
authors.lastname: Poczatek
authors.affiliation: Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA USA ; National Resource for Imaging Mass Spectrometry, Cambridge, MA USA.
authors.forename: C
authors.initials: C
authors.lastname: Lechene
keywords: Dendritic spines; MIMS; Multi-isotope Imaging Mass Spectrometry; Neurons; Protein synthesis; RNA