title: Analysis of metabolic pools in broilers chicks.
abstract: This paper shows the possibility of obtaining new parameters for the mathematical modelling of data on stable isotopes in biological systems and its application in obtaining data on metabolic pools of blood plasma, blood serum, liver and muscle of broilers. This theory states that the modelling of turnover used for studies of isotopic incorporation when the metabolism has a single metabolic pool is feasible by the technique of setting an exponential. However, when the metabolism has more than one metabolic pool, it is necessary to apply the linearization technique, linear regression adjustment and evaluation of the assumptions of regression to obtain the kinetic parameters such as half-life (T1/2) and isotope exchange rate (k). The application of this technique on carbon-13 data from 100 one-day-old chicks, with the change of diet composed of grains of the photosynthetic cycle of plants from C4 to C3, in broilers has enabled the discovery that the liver, blood plasma and blood serum have a single metabolic pool; however, the pectoral muscle has two metabolic pools. For the liver, blood plasma and blood serum, the half-life values were found by the exponential fit being T1/2 = 1.4 days with the rate of exchange of k = 0.502, T1/2 = 2.4 days with k = 0.293 and T1/2 = 2.0 days with k = 0.348, respectively. For the pectoral muscle, after linearization, the half-life values were found for T1/2(1) = 1.7 and T1/2(2) = 3 days, with exchange rates of k1 = 0.405 and k2 = 0.235, representing approximately 66 and 34%, respectively.
authors.affiliation: a Faculdade de Ciências Agronômicas , Universidade Estadual Paulista (UNESP) , Botucatu , São Paulo , Brazil.
authors.forename: Maria Márcia Pereira
authors.initials: MM
authors.lastname: Sartori
authors.affiliation: b Departamento de Física e Biofísica , Universidade Estadual Paulista (UNESP) , Botucatu , São Paulo , Brazil.
authors.forename: Juliana Célia
authors.initials: JC
authors.lastname: Denadai
authors.affiliation: c Departamento de Melhoramento e Nutrição Animal , Universidade Estadual Paulista (UNESP) , Botucatu , São Paulo , Brazil.
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Sartori
authors.affiliation: d Departamento de Morfologia e Fisiologia Animal , Universidade Estadual Paulista (UNESP) , Jaboticabal , São Paulo , Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Campos
authors.affiliation: d Departamento de Morfologia e Fisiologia Animal , Universidade Estadual Paulista (UNESP) , Jaboticabal , São Paulo , Brazil.
authors.forename: Marcos
authors.initials: M
authors.lastname: Macari
authors.affiliation: c Departamento de Melhoramento e Nutrição Animal , Universidade Estadual Paulista (UNESP) , Botucatu , São Paulo , Brazil.
authors.forename: Antônio Celso
authors.initials: AC
authors.lastname: Pezzato
authors.affiliation: b Departamento de Física e Biofísica , Universidade Estadual Paulista (UNESP) , Botucatu , São Paulo , Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Ducatti
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002244:Carbon / Q000097:blood / Q000378:metabolism*; D002247:Carbon Isotopes / Q000032:analysis; D002645:Chickens / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D006207:Half-Life; D007700:Kinetics; D008099:Liver / Q000737:chemistry; D008962:Models, Theoretical; D010369:Pectoralis Muscles / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D002247:Carbon Isotopes; D002244:Carbon
keywords: animals; carbon-13; exponential adjustment; isotope biochemistry; isotope ecology; linear analysis; modelling; natural isotope variations