title: Icaritin inhibits the invasion and epithelial-to-mesenchymal transition of glioblastoma cells by targeting EMMPRIN via PTEN/AKt/HIF-1α signalling.
abstract: Icaritin, a hydrolytic product of icariin from the Epimedium genus, exerts anti-tumour effects on a variety of tumour cell types, mainly by inhibiting cell proliferation and inducing apoptosis. However, little is known about the role of icaritin in cancer invasion and epithelial-to-mesenchymal transition (EMT). In the present study, the glioblastoma (GBM) cell line U87MG was used as a model to investigate the effects of icaritin on the invasion and EMT of cancer cells. The results showed that icaritin significantly inhibited the invasion and EMT of GBM cells by targeting extracellular matrix metalloproteinase (EMMPRIN). Furthermore, the findings strongly indicate that the modulatory effect of icaritin on EMMPRIN is mediated via the PTEN/Akt/HIF-1α signalling pathway. The data provide the first experimental evidence of the inhibitory effect of icaritin on cancer cell invasion and EMT, thus highlighting the potential of icaritin to be employed as a promising anti-cancer agent in the treatment of GBM.
authors.affiliation: Department of Neurology, Provincial Hospital of Shandong University, Jinan, Shandong, China.|Department of Neurology, The Second Affiliated Hospital, Medical College of Qingdao University, Qingdao, Shandong, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Neurology and Department of Neurosurgery, Qingdao Hiser Medical Centre, Qingdao, Shandong, China.
authors.forename: Chuanwu
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Department of Neurology, Qilu Hospital of Shandong University, Jinan and Department of Neurology Linyi People's Hospital, Linyi, Shandong, China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Han
authors.affiliation: Department of Neurology and Department of Neurosurgery, Qingdao Hiser Medical Centre, Qingdao, Shandong, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Neurology and Department of Neurosurgery, Qingdao Hiser Medical Centre, Qingdao, Shandong, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Tang
authors.affiliation: Department of Neurology and Department of Neurosurgery, Qingdao Hiser Medical Centre, Qingdao, Shandong, China.
authors.forename: Longxi
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Neurology and Department of Neurosurgery, Qingdao Hiser Medical Centre, Qingdao, Shandong, China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Ji
authors.affiliation: Department of Neurology and Department of Neurosurgery, Qingdao Hiser Medical Centre, Qingdao, Shandong, China.
authors.forename: Xuechao
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Neurology and Department of Neurosurgery, Qingdao Hiser Medical Centre, Qingdao, Shandong, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Neurology and Department of Neurosurgery, Qingdao Hiser Medical Centre, Qingdao, Shandong, China.
authors.forename: Yunxu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurology and Department of Neurosurgery, Qingdao Hiser Medical Centre, Qingdao, Shandong, China.
authors.forename: Yongji
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D051926:Basigin / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D005419:Flavonoids / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D055785:Gene Knockdown Techniques; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D058990:Molecular Targeted Therapy; D009361:Neoplasm Invasiveness; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D005419:Flavonoids; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051926:Basigin; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; C499403:icaritin
keywords: Akt; HIF-1α; extracellular matrix metalloproteinase; glioblastoma; icaritin