title: Significance of caveolin-1 and matrix metalloproteinase 14 gene expression in canine mammary tumours.
abstract: Canine mammary tumours (CMTs) are the most common neoplasms affecting female dogs. There is an urgent need for molecular biomarkers that can detect early stages of the disease in order to improve accuracy of CMT diagnosis. The aim of this study was to examine whether caveolin-1 (Cav-1) and matrix metalloproteinase 14 (MMP14) are associated with CMT histological malignancy and invasion. Sixty-five benign and malignant CMT samples and six normal canine mammary glands were analysed using quantitative reverse transcription-polymerase chain reaction. Cav-1 and MMP14 genes were highly expressed in CMT tissues compared to normal tissues. Cav-1 especially was overexpressed in malignant and invasive CMT tissues. When a CMT cell line was cultured on fluorescent gelatin-coated coverslips, localisation of Cav-1 was observed at invadopodia-mediated degradation sites of the gelatin matrix. These findings suggest that Cav-1 may be involved in CMT invasion and that the markers may be useful for estimating CMT malignancy.
authors.affiliation: Laboratory of Veterinary Biochemistry, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ebisawa
authors.affiliation: Laboratory of Veterinary Biochemistry, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. Electronic address: h-iwano@rakuno.ac.jp.
authors.forename: H
authors.initials: H
authors.lastname: Iwano
authors.affiliation: Laboratory of Veterinary Biochemistry, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nishikawa
authors.affiliation: Laboratory of Veterinary Physiology, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tochigi
authors.affiliation: Laboratory of Veterinary Clinical Oncology, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Komatsu
authors.affiliation: Laboratory of Veterinary Clinical Oncology, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Endou
authors.affiliation: Laboratory of Veterinary Pathology, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hirayama
authors.affiliation: Laboratory of Veterinary Pathology, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Taniyama
authors.affiliation: Laboratory of Veterinary Clinical Oncology, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kadosawa
authors.affiliation: Laboratory of Veterinary Biochemistry, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yokota
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism; D016022:Case-Control Studies; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D015674:Mammary Neoplasms, Animal / Q000378:metabolism*; D053511:Matrix Metalloproteinase 14 / Q000235:genetics / Q000378:metabolism*
chemical_list: D051242:Caveolin 1; D053511:Matrix Metalloproteinase 14
keywords: Canine mammary tumours; Caveolin-1; Invadopodia; Malignancy