title: Zebrafish neurobehavioral phenomics for aquatic neuropharmacology and toxicology research.
abstract: Zebrafish (Danio rerio) are rapidly emerging as an important model organism for aquatic neuropharmacology and toxicology research. The behavioral/phenotypic complexity of zebrafish allows for thorough dissection of complex human brain disorders and drug-evoked pathological states. As numerous zebrafish models become available with a wide spectrum of behavioral, genetic, and environmental methods to test novel drugs, here we discuss recent zebrafish phenomics methods to facilitate drug discovery, particularly in the field of biological psychiatry. Additionally, behavioral, neurological, and endocrine endpoints are becoming increasingly well-characterized in zebrafish, making them an inexpensive, robust and effective model for toxicology research and pharmacological screening. We also discuss zebrafish behavioral phenotypes, experimental considerations, pharmacological candidates and relevance of zebrafish neurophenomics to other 'omics' (e.g., genomic, proteomic) approaches. Finally, we critically evaluate the limitations of utilizing this model organism, and outline future strategies of research in the field of zebrafish phenomics.
authors.affiliation: Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong 524025, China; The International Zebrafish Neuroscience Research Consortium (ZNRC), Slidell, LA 70458, USA; ZENEREI Institute, 309 Palmer Court, Slidell, LA 70458, USA; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg 199034, Russia; Chemical-Technological Institute and Institute of Natural Sciences, Ural Federal University, Ekaterinburg 620002, Russia. Electronic address: avkalueff@gmail.com.
authors.forename: Allan V
authors.initials: AV
authors.lastname: Kalueff
authors.affiliation: The International Zebrafish Neuroscience Research Consortium (ZNRC), Slidell, LA 70458, USA; Department of Psychology, University of Southern Mississippi, 118 College Drive, Hattiesburg, MS 39406, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Echevarria
authors.affiliation: Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Sumit
authors.initials: S
authors.lastname: Homechaudhuri
authors.affiliation: The International Zebrafish Neuroscience Research Consortium (ZNRC), Slidell, LA 70458, USA; ZENEREI Institute, 309 Palmer Court, Slidell, LA 70458, USA.
authors.forename: Adam Michael
authors.initials: AM
authors.lastname: Stewart
authors.affiliation: The International Zebrafish Neuroscience Research Consortium (ZNRC), Slidell, LA 70458, USA; Department of Psychology, University of Southern Mississippi, 118 College Drive, Hattiesburg, MS 39406, USA.
authors.forename: Adam D
authors.initials: AD
authors.lastname: Collier
authors.affiliation: ZENEREI Institute, 309 Palmer Court, Slidell, LA 70458, USA.
authors.forename: Aleksandra A
authors.initials: AA
authors.lastname: Kaluyeva
authors.affiliation: Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong 524025, China.
authors.forename: Shaomin
authors.initials: S
authors.lastname: Li
authors.affiliation: Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong 524025, China.
authors.forename: Yingcong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong 524025, China.
authors.forename: Peirong
authors.initials: P
authors.lastname: Chen
authors.affiliation: Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong 524025, China.
authors.forename: JiaJia
authors.initials: J
authors.lastname: Wang
authors.affiliation: Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong 524025, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Anisa
authors.initials: A
authors.lastname: Mitra
authors.affiliation: Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Subharthi
authors.initials: S
authors.lastname: Pal
authors.affiliation: Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Adwitiya
authors.initials: A
authors.lastname: Chaudhuri
authors.affiliation: Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Anwesha
authors.initials: A
authors.lastname: Roy
authors.affiliation: Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Missidona
authors.initials: M
authors.lastname: Biswas
authors.affiliation: Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Dola
authors.initials: D
authors.lastname: Roy
authors.affiliation: Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Anupam
authors.initials: A
authors.lastname: Podder
authors.affiliation: The International Zebrafish Neuroscience Research Consortium (ZNRC), Slidell, LA 70458, USA; ZENEREI Institute, 309 Palmer Court, Slidell, LA 70458, USA.
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Poudel
authors.affiliation: Proteomics and Translational Research Lab, Centre for Medical Biotechnology, Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida 201303, UP, India.
authors.forename: Deepshikha P
authors.initials: DP
authors.lastname: Katare
authors.affiliation: Proteomics and Translational Research Lab, Centre for Medical Biotechnology, Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida 201303, UP, India.
authors.forename: Ruchi J
authors.initials: RJ
authors.lastname: Mani
authors.affiliation: The International Zebrafish Neuroscience Research Consortium (ZNRC), Slidell, LA 70458, USA; Department of Psychiatry, Psychiatric Institute, University of Illinois at Chicago, 1601 W Taylor St., Chicago, IL 60612, USA.
authors.forename: Evan J
authors.initials: EJ
authors.lastname: Kyzar
authors.affiliation: Graduate Institute of Neural and Cognitive Sciences, China Medical University Hospital, Taichung 40402, Taiwan.
authors.forename: Siddharth
authors.initials: S
authors.lastname: Gaikwad
authors.affiliation: The International Zebrafish Neuroscience Research Consortium (ZNRC), Slidell, LA 70458, USA; ZENEREI Institute, 309 Palmer Court, Slidell, LA 70458, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Nguyen
authors.affiliation: Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong 524025, China; Graduate Institute of Neural and Cognitive Sciences, China Medical University Hospital, Taichung 40402, Taiwan.
authors.forename: Cai
authors.initials: C
authors.lastname: Song
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D006801:Humans; D023421:Models, Animal; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D040901:Proteomics; D011619:Psychotropic Drugs / Q000633:toxicity; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity; D015027:Zebrafish / Q000502:physiology*
chemical_list: D011619:Psychotropic Drugs; D014874:Water Pollutants, Chemical
keywords: Aquatic model; Behavior; Ecotoxicology; Neurophenomics; Neuropsychopharmacology; Zebrafish