title: Zebrafish jam-b2 Gal4-enhancer trap line recapitulates endogenous jam-b2 expression in extraocular muscles.
abstract: BACKGROUND
  Members of the junctional adhesion molecule (JAM) family function as cell adhesion molecules and cell surface receptors. The zebrafish genome contains six different jam genes, and jam-b and jam-c were shown to be essential for myoblast fusion during skeletal muscle development. However, little is known about jam-b2 expression and function.
  
  
  RESULTS
  We isolated the cDNA of zebrafish jam-b2. jam-b2 is expressed specifically in extraocular muscles (EOMs), jaw muscles, and pectoral fins in zebrafish larvae, but not in trunk muscles. The identified jam-b2 expression pattern is supported by the analysis of a zebrafish Gal4-enhancer trap line, in which the coding sequence of the transcriptional activator KalTA4 together with a Gal4-dependent UAS-mCherry expression cassette was inserted into the jam-b2 locus. Intercrosses with an UAS:EGFP strain proves the possibility for targeting transgene expression to EOMs, jaw muscles and fins. Finally, we characterized the concerted contraction pattern of EOMs in larvae performing an optokinetic response.
  
  
  CONCLUSIONS
  The expression pattern of jam-b2 suggests that it may contribute different properties to EOMs, jaw muscles, and pectoral fins. The jam-b2:KalTA4-UAS-mCherry transgenic strain serves a dual role as both a reporter for these muscles and as a valuable genetic tool for targeting transgene expression to EOMs.
authors.affiliation: Division of Molecular and Cellular Neurobiology, Zoological Institute, Technical University Braunschweig, Braunschweig, Germany.|Department of Neuroscience, Section of Integrative Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsui
authors.affiliation: Division of Molecular and Cellular Neurobiology, Zoological Institute, Technical University Braunschweig, Braunschweig, Germany.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Dorigo
authors.affiliation: Division of Molecular and Cellular Neurobiology, Zoological Institute, Technical University Braunschweig, Braunschweig, Germany.
authors.forename: Astrid
authors.initials: A
authors.lastname: Buchberger
authors.affiliation: Division of Molecular and Cellular Neurobiology, Zoological Institute, Technical University Braunschweig, Braunschweig, Germany.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Hocking
authors.affiliation: Division of Molecular and Cellular Neurobiology, Zoological Institute, Technical University Braunschweig, Braunschweig, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Distel
authors.affiliation: Division of Molecular and Cellular Neurobiology, Zoological Institute, Technical University Braunschweig, Braunschweig, Germany.
authors.forename: Reinhard W
authors.initials: RW
authors.lastname: Köster
mesh_terms: D058500:Animal Fins / Q000196:embryology / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D018507:Gene Expression Regulation, Developmental; D062765:Junctional Adhesion Molecule B / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000196:embryology / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D062765:Junctional Adhesion Molecule B; D029961:Zebrafish Proteins
keywords: extraocular muscle; junctional adhesion molecule; zebrafish