title: Evaluation of inhibition of F4ac positive Escherichia coli attachment with xanthine dehydrogenase, butyrophilin, lactadherin and fatty acid binding protein.
abstract: BACKGROUND
  Neonatal and post-weaning colibacillosis caused by enterotoxigenic E. coli is responsible for substantial economic losses encountered by the pork industry. Intestinal colonization of young piglets by E. coli depends on the efficiency of bacterial attachment to host gastrointestinal epithelium that is mediated by fimbriae. We tested the effect of porcine individual milk fat globule membrane (MFGM) proteins on F4ac positive E. coli attachment to porcine enterocytes in vitro.
  
  
  RESULTS
  Butyrophilin, lactadherin and fatty acid binding protein inhibited fimbriae-dependent adherence of E. coli to enterocytes in vitro, while xanthine dehydrogenase did not. The inhibiting activity was dose-dependent for all three proteins, but the inhibiting efficiency was different.
  
  
  CONCLUSIONS
  The results indicate that MFGM proteins may interfere with attachment of E. coli to porcine neonatal intestinal mucosa.
authors.affiliation: Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada. predrag.novakovic@usask.ca.
authors.forename: Predrag
authors.initials: P
authors.lastname: Novakovic
authors.affiliation: Western University of Health Sciences, College of Veterinary Medicine, 309 East Second Street, Pomona, CA, 91766, USA. ccharavaryamath@westernu.edu.
authors.forename: Chandrashekhar
authors.initials: C
authors.lastname: Charavaryamath
authors.affiliation: Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada. igor.moshynskyy@usask.ca.
authors.forename: Igor
authors.initials: I
authors.lastname: Moshynskyy
authors.affiliation: Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada. betty.lockerbie@usask.ca.
authors.forename: Betty
authors.initials: B
authors.lastname: Lockerbie
authors.affiliation: Biology & Microbiology-Box 2140D, South Dakota State University, Brookings, SD, 57007, USA. Radhey.Kaushik@sdstate.edu.
authors.forename: Radhey S
authors.initials: RS
authors.lastname: Kaushik
authors.affiliation: Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada. matthew.loewen@usask.ca.
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Loewen
authors.affiliation: Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada. beverly.kidney@usask.ca.
authors.forename: Beverly A
authors.initials: BA
authors.lastname: Kidney
authors.affiliation: Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada. c.p.stuart@gmail.com.
authors.forename: Chris
authors.initials: C
authors.lastname: Stuart
authors.affiliation: Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada. elemir.simko@usask.ca.
authors.forename: Elemir
authors.initials: E
authors.lastname: Simko
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000187:drug effects*; D000071797:Butyrophilins; D002460:Cell Line; D020895:Enterocytes; D054307:Enterotoxigenic Escherichia coli / Q000187:drug effects / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000008:administration & dosage / Q000494:pharmacology*; D008894:Milk Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D013552:Swine; D014968:Xanthine Dehydrogenase / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000942:Antigens, Bacterial; D000071797:Butyrophilins; D029968:Escherichia coli Proteins; D050556:Fatty Acid-Binding Proteins; C026888:K88 antigen, E coli; D008562:Membrane Glycoproteins; D008894:Milk Proteins; D036781:Fimbriae Proteins; D014968:Xanthine Dehydrogenase