title: Controlled Transcription of Exogenous mRNA in Platelets Using Protocells.
abstract: Transcribing exogenous RNA in eukaryotic cells requires delivering DNA to their nuclei and changing their genome. Nuclear delivery is often inefficient, limiting the potential scope of gene therapy and synthetic biology. These challenges may be overcome by techniques that allow for extranucleate transcription within eukaryotic cells. Protocells have been developed that enable transcription inside of liposomes; however, it has not yet been demonstrated whether this technology can be extended for use within eukaryotic cells. Here we show RNA-synthesizing nanoliposomes allow transcription of exogenous RNA inside anucleate cells. To accomplish this, components of transcription were encapsulated into liposomes and delivered to platelets. These liposomes were capable of light-induced transcription in platelets, providing proof-of-concept that protocell technology can be adapted for use within mammalian cells.
authors.affiliation: Michael Smith Laboratories, University of British Columbia (Canada).|Biochemistry and Molecular Biology, University of British Columbia (Canada).|Centre for Blood Research, University of British Columbia (Canada).
authors.forename: Vivienne
authors.initials: V
authors.lastname: Chan
authors.affiliation: Michael Smith Laboratories, University of British Columbia (Canada).|Biochemistry and Molecular Biology, University of British Columbia (Canada).|Centre for Blood Research, University of British Columbia (Canada).
authors.forename: Stefanie K
authors.initials: SK
authors.lastname: Novakowski
authors.affiliation: Biochemistry and Molecular Biology, University of British Columbia (Canada).|Centre for Blood Research, University of British Columbia (Canada).
authors.forename: Simon
authors.initials: S
authors.lastname: Law
authors.affiliation: Centre for Blood Research, University of British Columbia (Canada).|Pathology and Laboratory Medicine, University of British Columbia (Canada).
authors.forename: Christa
authors.initials: C
authors.lastname: Klein-Bosgoed
authors.affiliation: Michael Smith Laboratories, University of British Columbia (Canada). ckastrup@mail.ubc.ca.|Biochemistry and Molecular Biology, University of British Columbia (Canada). ckastrup@mail.ubc.ca.|Centre for Blood Research, University of British Columbia (Canada). ckastrup@mail.ubc.ca.
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Kastrup
mesh_terms: D058045:Artificial Cells / Q000378:metabolism*; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D008081:Liposomes; D012333:RNA, Messenger
keywords: drug delivery; liposomes; nanomedicine; synthetic biology; transfection