title: Low Counts of B Cells, Natural Killer Cells, Monocytes, Dendritic Cells, Basophils, and Eosinophils are Associated with Postengraftment Infections after Allogeneic Hematopoietic Cell Transplantation.
abstract: Hematopoietic cell transplant (HCT) recipients are immunocompromised and thus predisposed to infections. We set out to determine the deficiency of which immune cell subset(s) may predispose to postengraftment infections. We determined day 28, 56, 84, and 180 blood counts of multiple immune cell subsets in 219 allogeneic transplant recipients conditioned with busulfan, fludarabine, and Thymoglobulin. Deficiency of a subset was considered to be associated with infections if the low subset count was significantly associated with subsequent high infection rate per multivariate analysis in both discovery and validation cohorts. Low counts of monocytes (total and inflammatory) and basophils, and low IgA levels were associated with viral infections. Low plasmacytoid dendritic cell (PDC) counts were associated with bacterial infections. Low inflammatory monocyte counts were associated with fungal infections. Low counts of total and naive B cells, total and CD56(high) natural killer (NK) cells, total and inflammatory monocytes, myeloid dendritic cells (MDCs), PDCs, basophils and eosinophils, and low levels of IgA were associated with any infections (due to any pathogen or presumed). In conclusion, deficiencies of B cells, NK cells, monocytes, MDCs, PDCs, basophils, eosinophils, and/or IgA plasma cells appear to predispose to postengraftment infections.
authors.affiliation: Department of Medicine, University of Calgary, Calgary, Alberta, Canada. Electronic address: podgorny@ualberta.ca.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Podgorny
authors.affiliation: Department of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Pratt
authors.affiliation: Department of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Poonam
authors.initials: P
authors.lastname: Dharmani-Khan
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Joanne
authors.initials: J
authors.lastname: Luider
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Iwona
authors.initials: I
authors.lastname: Auer-Grzesiak
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Adnan
authors.initials: A
authors.lastname: Mansoor
authors.affiliation: Department of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Tyler S
authors.initials: TS
authors.lastname: Williamson
authors.affiliation: Department of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Ugarte-Torres
authors.affiliation: Department of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Mette
authors.initials: M
authors.lastname: Hoegh-Petersen
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Faisal M
authors.initials: FM
authors.lastname: Khan
authors.affiliation: Department of Medicine, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Loree
authors.initials: L
authors.lastname: Larratt
authors.affiliation: Department of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Victor H
authors.initials: VH
authors.lastname: Jimenez-Zepeda
authors.affiliation: Department of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Stewart
authors.affiliation: Department of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: James A
authors.initials: JA
authors.lastname: Russell
authors.affiliation: Department of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Andrew
authors.initials: A
authors.lastname: Daly
authors.affiliation: Department of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Jan
authors.initials: J
authors.lastname: Storek
mesh_terms: D000328:Adult; D000368:Aged; D064591:Allografts; D005260:Female; D019337:Hematologic Neoplasms / Q000097:blood*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007239:Infections / Q000097:blood* / Q000209:etiology; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D019653:Myeloablative Agonists / Q000008:administration & dosage* / Q000009:adverse effects; D019172:Transplantation Conditioning
chemical_list: D019653:Myeloablative Agonists
keywords: Hematopoietic; Immune cells; Infections; Monocytes; Transplantation