title: Neonatal aphakia is associated with altered levels of dopamine metabolites in the non-human primate retina.
abstract: Neonatal aphakia is associated with retardation of the axial elongation of the neonatal eye. In contrast, form deprivation increases axial elongation, an effect that has been associated with decreased retinal dopamine metabolism. The present investigation was conducted to test the hypothesis that neonatal aphakia induces an effect on the levels of retinal dopamine opposite to form deprivation. Lensectomy and vitrectomy were performed on the right eyes of rhesus monkeys at approximately 1 week of age; their left eyes were unmanipulated. Axial length was measured by A-scan ultrasonography. Prior to surgery, mean axial length of the right and left eyes was identical. Following lens removal, both eyes continued to elongate, however the aphakic eyes elongated at a slower rate resulting in a significant shorter axial length compared to that of the unmanipulated eye. Removal of the crystalline lens had no effect on steady-state dopamine levels in either central or peripheral retina. However, levels of the dopamine metabolites, 3,4-dihydroxyphenylacetic acid and homovanillic acid were significantly elevated in central retina, but not in the peripheral retina of aphakic eyes. Our results support the hypothesis that dopamine is a component of the retinal signaling pathways that are involved in the regulation of eye growth and emmetropization.
authors.affiliation: Department of Ophthalamology, Emory University School of Medicine, Atlanta, GA 30322, USA; Department of Pharmacology, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: P Michael
authors.initials: PM
authors.lastname: Iuvone
authors.affiliation: Department of Ophthalamology, Emory University School of Medicine, Atlanta, GA 30322, USA; Department of Pharmacology, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Rashidul
authors.initials: R
authors.lastname: Haque
authors.affiliation: Department of Ophthalamology, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Alcides
authors.initials: A
authors.lastname: Fernandes
authors.affiliation: Department of Ophthalamology, Emory University School of Medicine, Atlanta, GA 30322, USA. Electronic address: slamber@emory.edu.
authors.forename: Scott R
authors.initials: SR
authors.lastname: Lambert
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001036:Aphakia, Postcataract / Q000378:metabolism*; D058452:Axial Length, Eye; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism*; D006719:Homovanillic Acid / Q000378:metabolism*; D007908:Lens, Crystalline / Q000601:surgery; D008253:Macaca mulatta; D009216:Myopia / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*; D015398:Signal Transduction; D014821:Vitrectomy
chemical_list: D015102:3,4-Dihydroxyphenylacetic Acid; D004298:Dopamine; D006719:Homovanillic Acid
keywords: Emmetropization; Eye growth; Neuromodulators; Primate; Retina