title: Biocompatibility, degradability, bioactivity and osteogenesis of mesoporous/macroporous scaffolds of mesoporous diopside/poly(L-lactide) composite.
abstract: Bioactive mesoporous diopside (m-DP) and poly(L-lactide) (PLLA) composite scaffolds with mesoporous/macroporous structure were prepared by the solution-casting and particulate-leaching method. The results demonstrated that the degradability and bioactivity of the mesoporous/macroporous scaffolds were significantly improved by incorporating m-DP into PLLA, and that the improvement was m-DP content-dependent. In addition, the scaffolds containing m-DP showed the ability to neutralize acidic degradation products and prevent the pH from dropping in the solution during the soaking period. Moreover, the scaffolds containing m-DP enhanced attachment, proliferation and alkaline phosphatase activity of MC3T3-E1 cells, which were also m-DP content-dependent. Furthermore, the histological and immunohistochemical analysis results showed that the scaffolds with m-DP significantly promoted new bone formation and improved the materials degraded in vivo, indicating good biocompatibility. The results suggested that the mesoporous/macroporous scaffolds of the m-DP/PLLA composite with osteogenesis had a potential for bone regeneration.
authors.affiliation: Shanghai Key Laboratory of Advanced Polymeric Materials, Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
authors.forename: Zhulin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Shanghai Key Laboratory of Advanced Polymeric Materials, Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
authors.forename: Jiajin
authors.initials: J
authors.lastname: Ji
authors.affiliation: Shanghai Key Laboratory of Advanced Polymeric Materials, Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, People's Republic of China schtang@ecust.edu.cn.
authors.forename: Songchao
authors.initials: S
authors.lastname: Tang
authors.affiliation: Shanghai Key Laboratory of Advanced Polymeric Materials, Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, People's Republic of China qianjun@ecust.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.affiliation: College of Physical Science and Technology, Sichuan University, Chengdu 610041, People's Republic of China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, People's Republic of China.
authors.forename: Baoqing
authors.initials: B
authors.lastname: Yu
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, People's Republic of China.
authors.forename: Jiacan
authors.initials: J
authors.lastname: Su
authors.affiliation: Shanghai Key Laboratory of Advanced Polymeric Materials, Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001031:Apatites / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001826:Body Fluids / Q000737:chemistry; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D005269:Femur / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D010012:Osteogenesis / Q000502:physiology*; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D011817:Rabbits; D012824:Silicic Acid / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction; D055114:X-Ray Microtomography
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D018786:Bone Substitutes; D011091:Polyesters; D012824:Silicic Acid; C074224:diopside; C033616:poly(lactide); D000469:Alkaline Phosphatase
keywords: biocomposite; degradability; mesoporous diopside; mesoporous/macroporous scaffolds; osteogenesis