title: Mass culture strategy for bacterial yeast co-culture for degradation of petroleum hydrocarbons in marine environment.
abstract: In the present study a metabolically versatile co-culture with two Bacilli and one yeast strain was developed using enrichment culture techniques. The developed co-culture had affinity to degrade both aliphatic and aromatic fractions of petroleum crude oil. Degradation kinetics was established for designing the fermentation protocol of the co-culture. The developed mass culture strategy led to achieve the reduction in surface tension (26dynescm(-1) from 69 dynescm(-1)) and degradation of 67% in bench scale experiments. The total crude oil degradation of 96% was achieved in 4000l of natural seawater after 28days without adding any nutrients. The survival of the augmented co-culture was maintained (10(9)cellsml(-1)) in contaminated marine environment. The mass culture protocol devised for the bioaugmentation was a key breakthrough that was subsequently used for pilot scale studies with 100l and 4000l of natural seawater for potential application in marine oil spills.
authors.affiliation: TERI University, 10 Institutional Area, Vasant Kunj, New Delhi 110 070, India; Environment and Industrial Biotechnology Division, The Energy and Resources Institute, India Habitat Centre, Darbari Seth Block, Lodhi Road, New Delhi, India.
authors.forename: Anchal
authors.initials: A
authors.lastname: Priya
authors.affiliation: TERI University, 10 Institutional Area, Vasant Kunj, New Delhi 110 070, India.
authors.forename: Ajoy K
authors.initials: AK
authors.lastname: Mandal
authors.affiliation: School of Applied Sciences, RMIT University, Bundoora West Campus, Melbourne, Australia.
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Ball
authors.affiliation: Centre for Marine Bio Innovation, University of New South Wales, Sydney, NSW, Australia.
authors.forename: Mike
authors.initials: M
authors.lastname: Manefield
authors.affiliation: TERI University, 10 Institutional Area, Vasant Kunj, New Delhi 110 070, India; Environment and Industrial Biotechnology Division, The Energy and Resources Institute, India Habitat Centre, Darbari Seth Block, Lodhi Road, New Delhi, India. Electronic address: banwaril@teri.res.in.
authors.forename: Banwari
authors.initials: B
authors.lastname: Lal
authors.affiliation: TERI University, 10 Institutional Area, Vasant Kunj, New Delhi 110 070, India; Environment and Industrial Biotechnology Division, The Energy and Resources Institute, India Habitat Centre, Darbari Seth Block, Lodhi Road, New Delhi, India. Electronic address: priyangshumsarma@yahoo.co.in.
authors.forename: Priyangshu M
authors.initials: PM
authors.lastname: Sarma
mesh_terms: D001407:Bacillus / Q000378:metabolism; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D002175:Candida / Q000378:metabolism; D018920:Coculture Techniques; D006838:Hydrocarbons / Q000378:metabolism; D007194:India; D059013:Microbial Consortia; D010578:Petroleum / Q000378:metabolism*; D059392:Petroleum Pollution; D012623:Seawater / Q000382:microbiology
chemical_list: D006838:Hydrocarbons; D010578:Petroleum
keywords: Aliphatic and aromatic hydrocarbons; Bioaugmentation; Biodegradation; Bioreactor; Mass-culture strategy; Seawater