title: Taxonomy proposal for Old World monkey adenoviruses: characterisation of several non-human, non-ape primate adenovirus lineages.
abstract: A species classification regarding Old World monkey adenoviruses is proposed. We determined the nucleotide sequences of PCR-amplified fragments from the genes of the IVa2, DNA-dependent DNA polymerase, penton base, and hexon proteins from every simian adenovirus (SAdV) serotype that originated from Old World monkeys for which the full genome sequence had not yet been published. We confirmed that the majority of Old Word monkey SAdVs belong to two previously established species. Interestingly, one is the most recently established human AdV species, Human mastadenovirus G, which includes a single human virus, HAdV-52, as well as SAdV-1, -2, -7, -11, -12, and -15. The other approved species, Simian mastadenovirus A includes SAdV-3, -4, -6, -9, -10, -14, and -48. Several SAdVs (SAdV-5, -8, -49, -50) together with baboon AdV-1 and rhesus monkey AdV strains A1139, A1163, A1173, A1258, A1285, A1296, A1312, A1327 and A1335 have been proposed to be classified into an additional species, Simian mastadenovirus B. Another proposed species, Simian mastadenovirus C has been described for SAdV-19, baboon AdV-2/4 and -3. Our study revealed the existence of four additional AdV lineages. The corresponding new candidate species are Simian mastadenovirus D (for SAdV-13), Simian mastadenovirus E (for SAdV-16), Simian mastadenovirus F (for SAdV-17 and -18), and Simian mastadenovirus G (for SAdV-20). Several biological and genomic properties, such as the host origin, haemagglutination profile, number of fibre genes, and G+C content of the genome, strongly support this classification. Three SAdV strains originating from the American Type Culture Collection turned out to be mixtures of at least two virus types, either of the same species (SAdV-12 and -15 types from Human mastadenovirus G) or of two different species (SAdV-5 types from Simian mastadenovirus B and Human mastadenovirus G).
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, P. O. Box 18, 1581, Budapest, Hungary.|Laboratory of Genome Sciences, Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Japan.
authors.forename: Laura
authors.initials: L
authors.lastname: Pantó
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, P. O. Box 18, 1581, Budapest, Hungary.
authors.forename: Iva I
authors.initials: II
authors.lastname: Podgorski
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, P. O. Box 18, 1581, Budapest, Hungary.
authors.forename: Máté
authors.initials: M
authors.lastname: Jánoska
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, P. O. Box 18, 1581, Budapest, Hungary.
authors.forename: Orsolya
authors.initials: O
authors.lastname: Márkó
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, P. O. Box 18, 1581, Budapest, Hungary. harrach.balazs@agrar.mta.hu.
authors.forename: Balázs
authors.identifier: http://orcid.org/0000-0002-1410-6469
authors.initials: B
authors.lastname: Harrach
mesh_terms: D000261:Adenoviruses, Simian / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D002520:Cercopithecidae / Q000821:virology; D016679:Genome, Viral; D058507:Host Specificity; D006801:Humans; D017921:Mastadenovirus / Q000145:classification / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny