title: [Effect of lipopolysaccharide on the stromal cell-derived factor-1 expression in human stem cells from apical papilla].
abstract: OBJECTIVE
  To investigate the expression of stromal cell-derived factor-1 (SDF-1) in human stem cells from apical papilla (SCAP), and to evaluate the effect of lipopolysaccharide (LPS) on SDF-1 expression by SCAP.
  
  
  METHODS
  SCAP were isolated from dental papilla of human immature third molars. The expression of SDF-1 was evaluated by reverse transcription-PCR (RT-PCR). After SCAP being exposed to different concentrations (0.1, 1.0, 10 mg/L) of LPS for 24 and 48 h, the effect of LPS on cell proliferation and gene expression of SDF-1 was investigated by cell counting kit-8 and real-time PCR respectively, while cells without LPS stimulation were considered as negative control.
  
  
  RESULTS
  LPS had no significant effect on SCAP proliferation until day 7. RT-PCR assays demonstrated that SCAP expressed SDF-1 mRNA. Different concentrations of LPS significantly promoted the SDF-1 expression in SCAP after 24 h (F = 12.102, P = 0.002) and 48 h (F = 39.054, P < 0.001) exposure, with relative gene expression ratio (experimental/control) increased to 1.4 ± 0.1, 2.2 ± 0.4, 2.3 ± 0.5 in 24 h group and 2.1 ± 0.4, 3.4 ± 0.3, 3.8 ± 0.5 in 48 h group.
  
  
  CONCLUSIONS
  Isolated SCAP in cultures have the expression of SDF-1 mRNA. LPS can significantly promote the expression of SDF-1 in SCAP.
authors.affiliation: Department of Cariology, Endodontology and Operative Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Cariology, Endodontology and Operative Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zou
authors.affiliation: Department of Cariology, Endodontology and Operative Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Cariology, Endodontology and Operative Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Cariology, Endodontology and Operative Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China; Email: kqlinyue@bjmu.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Yue
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects*; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism*; D003771:Dental Papilla / Q000166:cytology*; D015870:Gene Expression; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D013234:Stem Cells / Q000187:drug effects* / Q000378:metabolism
chemical_list: D054377:Chemokine CXCL12; D008070:Lipopolysaccharides