title: Quantitative Determination of Tenuazonic Acid in Pig and Broiler Chicken Plasma by LC-MS/MS and Its Comparative Toxicokinetics.
abstract: A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantitate tenuazonic acid (TeA) in pig and broiler chicken plasma was successfully developed and validated. Linear matrix-matched calibration curves ranged between 5 and 200 ng/mL. Correlation coefficients, goodness-of-fit coefficients, and within-day and between-day precision and accuracy fell well within the acceptance criteria. The limit of quantitation was 5.0 ng/mL in both pig and broiler chicken plasma. The LC-MS/MS method was applied in a comparative toxicokinetic study in both pigs and broiler chickens. TeA was completely bioavailable after oral administration in both animal species. However, absorption was deemed to be slower in broiler chickens (mean tmax 0.32 h in pigs vs 2.60 h in chickens). TeA was more slowly eliminated in broiler chickens (mean t1/2el 0.55 h in pigs vs 2.45 h in chickens after oral administration), mainly due to the significantly lower total body clearance (mean Cl 446.1 mL/h/kg in pigs vs 59.2 mL/h/kg in chickens after oral administration). Tissue residue studies and further research to elucidate the biotransformation and excretion processes of TeA in pigs, broiler chickens, and other animal species are imperative.
authors.forename: Sophie
authors.initials: S
authors.lastname: Fraeyman
authors.forename: Mathias
authors.initials: M
authors.lastname: Devreese
authors.forename: Nathan
authors.initials: N
authors.lastname: Broekaert
authors.forename: Thomas
authors.initials: T
authors.lastname: De Mil
authors.forename: Gunther
authors.initials: G
authors.lastname: Antonissen
authors.forename: Siegrid
authors.initials: S
authors.lastname: De Baere
authors.forename: Patrick
authors.initials: P
authors.lastname: De Backer
authors.affiliation: Chair of Analytical Food Chemistry, Technische Universität München , Alte Akademie 10, 85354 Freising, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Rychlik
authors.forename: Siska
authors.initials: S
authors.lastname: Croubels
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009183:Mycotoxins / Q000097:blood* / Q000378:metabolism / Q000633:toxicity*; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013720:Tenuazonic Acid / Q000097:blood* / Q000378:metabolism / Q000633:toxicity*; D066007:Toxicokinetics
chemical_list: D009183:Mycotoxins; D013720:Tenuazonic Acid
keywords: LC-MS/MS; broiler chicken; oral bioavailability; pig; tenuazonic acid; toxicokinetics