title: Novel Strategy for Tracking the Microbial Degradation of Azo Dyes with Different Polarities in Living Cells.
abstract: Direct visualization evidence is important for understanding the microbial degradation mechanisms. To track the microbial degradation pathways of azo dyes with different polar characterizations, sensors based on the fluorescence resonance energy transfer (FRET) from 1,8-naphthalimide to azo dyes were synthesized, in which the quenched fluorescence will recover when the azo bond was cleaved. In living cells, the sensor-tracking experiment showed that the low polarity and hydrophobic azo dye can be taken up into the cells and reduced inside the cells, whereas the high polarity and hydrophilic azo dye can be reduced only outside the cells because of the selective permeability of the cell membranes. These results indicated that there were two different bacterial degradation pathways available for different polarity azo dyes. To our knowledge, no fluorescent sensor has yet been designed for illuminating the microbial degradation mechanisms of organic pollutants with different characteristics.
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology , 100 Central Xianlie Road, Guangzhou, Guangdong 510070, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology , 100 Central Xianlie Road, Guangzhou, Guangdong 510070, People's Republic of China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology , 100 Central Xianlie Road, Guangzhou, Guangdong 510070, People's Republic of China.
authors.forename: Xingjuan
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology , 100 Central Xianlie Road, Guangzhou, Guangdong 510070, People's Republic of China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology , 100 Central Xianlie Road, Guangzhou, Guangdong 510070, People's Republic of China.
authors.forename: Haiji
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology , 100 Central Xianlie Road, Guangzhou, Guangdong 510070, People's Republic of China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Sun
mesh_terms: D001391:Azo Compounds / Q000737:chemistry* / Q000378:metabolism*; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D057927:Hydrophobic and Hydrophilic Interactions; D053858:Metabolic Networks and Pathways; D020592:Shewanella / Q000378:metabolism*
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents