title: Cardioprotective role of tadalafil against cisplatin-induced cardiovascular damage in rats.
abstract: The present study investigated the possible cardioprotective effect of tadalafil (Tad) on cisplatin (CDDP)-induced cardiac and vascular damages in rats. A total number of seventy two healthy male albino rats initially weighting between 200 and 220 g were used and randomly divided into four groups,18 rats in each. The control group received no treatment; CDDP group received a single dose of CDDP (4 mg/kg) intraperitoneal (i.p.) per week for 4 weeks the duration of the experiment; Tad group received 0.4 mg/kg BW Tad i.p. daily and Tad +CDDP group received 0.4 mg/kg BW Tad i.p. +4 mg/kg BW CDDP i.p. The results showed that Tad was able to decrease blood pressure, heart rate, levels of serum cardiac troponin (cTn-I), malondialdehyde (MDA) and increased levels of reduced glutathione (GSH) and nitric oxide (NO) in the heart homogenate sample from CDDP treated rats. Semi-quantitative analysis showed that Tad was able to decrease the histopathological scores of cardiac muscular hyalinzation and fibrosis in three sacrifices in CDDP treated rats. CDDP treated rats showed significantly increased thickening in wall of aorta with an irregular luminal layer of endothelial cell linings in three sacrifices when it was compared to other groups. Moreover, immunohistochemical labeling of α- smooth muscle actin (α-SMA) in aorta revealed significant lower scores in Tad +CDDP group when they were compared to CDDP group. In conclusion, Tad alone did not induce any harmful effects on blood pressure, selective antioxidant, peroxidation markers or cardiac histology, in addition, Tad has a cardio-protective role against CDDP.
authors.affiliation: Department of Animal Physiology, Faculty of Veterinary Medicine, Mansoura University, Egypt.
authors.forename: Rasha M
authors.initials: RM
authors.lastname: Saleh
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Mansoura University, Egypt.
authors.forename: Walaa F
authors.initials: WF
authors.lastname: Awadin
authors.affiliation: Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt. Electronic address: dr_reham16@yahoo.com.
authors.forename: Reham A
authors.initials: RA
authors.lastname: El-Shafei
authors.affiliation: Department of Animal Physiology, Faculty of Veterinary Medicine, Mansoura University, Egypt.
authors.forename: Yousef Y
authors.initials: YY
authors.lastname: Elseady
authors.affiliation: Department of Animal Physiology, Faculty of Veterinary Medicine, Mansoura University, Egypt.
authors.forename: Faheim E
authors.initials: FE
authors.lastname: Wehaish
authors.affiliation: Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Egypt; Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Mohamed F
authors.initials: MF
authors.lastname: Elshal
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D002316:Cardiotonic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002318:Cardiovascular Diseases / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D002945:Cisplatin / Q000037:antagonists & inhibitors / Q000633:toxicity*; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D008297:Male; D051381:Rats; D000068581:Tadalafil / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D002316:Cardiotonic Agents; D000068581:Tadalafil; D002945:Cisplatin
keywords: Cardiac; Cisplatin; Rats; Tadalafil; Vascular damage