title: [miR-199a-5p inhibits the proliferation of rat airway smooth muscle cells and the expression of hypoxia inducible factor 1 alpha under hypoxia conditions].
abstract: OBJECTIVE
  To investigate the expression of miR-199a-5p in airway smooth muscle cells (ASMCs) of rats in normoxia and hypoxia and its role in the regulation of proliferation and the expression of hypoxia inducible factor 1 alpha (HIF-1α) of ASMCs.
  
  
  METHODS
  ASMCs were prepared by means of adherent culture in vitro. After ASMCs were cultured under normoxia and hypoxia conditions for 24 hours, the content of miR-199a-5p was detected by real-time quantitative PCR (qRT-PCR). The mimic or inhibitor of miR-199a-5p were artificially synthesized and transferred into ASMCs in hypoxia via liposomes. The expressions of miR-199a-5p and HIF-1α mRNA were detected by qRT-PCR. Western blotting and CCK-8 assay were applied to detect the expression levels of HIF-1α protein and the proliferation of ASMCs, respectively.
  
  
  RESULTS
  Compared with the normoxia group, hypoxia significantly promoted cell proliferation and increased the levels of HIF-1α mRNA and protein. The level of miR-199a-5p decreased in the hypoxia group compared with the normoxia group. The proliferation rate of ASMCs under hypoxia conditions was significantly attenuated by transfection of miR-199a-5p mimic, while it was significantly lifted by transfection of miR-199a-5p inhibitor. Compared with control group, the expression of HIF-1α protein was reduced in the mimic group and raised in the inhibitor group. There was no significant difference in the content of HIF-1α mRNA among groups under hypoxia conditions.
  
  
  CONCLUSIONS
  miR-199a-5p can inhibit the proliferation of ASMCs and the expression of HIF-1α protein in vitro under hypoxia conditions.
authors.affiliation: Department of Respiratory Medicine, Hospital Affiliated to Xuzhou Medical College, Xuzhou 221006, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Hospital Affiliated to Xuzhou Medical College, Xuzhou 221006, China.
authors.forename: Shuyang
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Respiratory Medicine, Hospital Affiliated to Xuzhou Medical College, Xuzhou 221006, China.
authors.forename: Cantang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Hospital Affiliated to Xuzhou Medical College, Xuzhou 221006, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, Hospital Affiliated to Xuzhou Medical College, Xuzhou 221006, China.
authors.forename: Jiechen
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001980:Bronchi / Q000378:metabolism*; D015687:Cell Hypoxia; D049109:Cell Proliferation; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C552425:MIRN199 microRNA, rat; D035683:MicroRNAs; D012333:RNA, Messenger