title: [Effects of calcium hydroxide and Hoshino's paste on the proliferation of porcine dental papilla cells].
abstract: OBJECTIVE
  To observe the effects of calcium hydroxide Ca(OH)₂and Hoshino's paste(a mixture of metronidazole, ciprofloxacin, and minocycline) on the proliferation of porcine dental papilla cells (pDPCs), and to provide some theoretical basis for the choice of root canal disinfection drugs for pulp revascularization treatment.
  
  
  METHODS
  The 4th generation of pDPCs were treated with several concentrations of Ca(OH)₂(10%,1%,0.1%) and Hoshino's paste (0.5%, 0.1%, 0.05%) medium for certain time periods. CCK-8 assay was performed to detect the effect of the 2 drugs on cell proliferation. SPSS19.0 software package was used for one-way ANOVA.
  
  
  RESULTS
  The effect of Ca(OH)₂on pDPCs proliferation was fluctuating, but cell proliferation of each Ca(OH)₂group had no difference after 9 days. Meanwhile, cell proliferation was inhibited by Hoshino's paste.
  
  
  CONCLUSIONS
  Each concentration of Ca(OH)₂in the study has no effect on pDPCs proliferation, while Hoshino's paste can inhibit cell proliferation.
authors.affiliation: Department of Pediatric Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China.E-mail:qiusihui1988@126.com.
authors.forename: Si-hui
authors.initials: SH
authors.lastname: Qiu
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002939:Ciprofloxacin / Q000494:pharmacology; D003771:Dental Papilla / Q000187:drug effects*; D008795:Metronidazole / Q000494:pharmacology; D008911:Minocycline / Q000494:pharmacology; D009824:Ointments; D012388:Root Canal Irrigants; D013552:Swine
chemical_list: D000890:Anti-Infective Agents; D009824:Ointments; D012388:Root Canal Irrigants; D008795:Metronidazole; D002939:Ciprofloxacin; D008911:Minocycline; D002126:Calcium Hydroxide