title: Identification of genes bordering breakpoints of the pericentric inversions on 2B, 4B, and 5A in bread wheat (Triticum aestivum L.).
abstract: Chromosome translocation is an important driving force in shaping genomes during evolution. Detailed knowledge of chromosome translocations in a given species and its close relatives should increase the efficiency and precision of chromosome engineering in crop improvement. To identify genes flanking the breakpoints of translocations and inversions as a step toward identifying breakpoints in bread wheat, we systematically analysed genes in the Brachypodium genome against wheat survey sequences and bin-mapped ESTs (expressed sequence tags) derived from the hexaploid wheat genotype 'Chinese Spring'. In addition to those well-known translocations between group 4, 5, and 7 chromosomes, this analysis identified genes flanking the three pericentric inversions on chromosomes 2B, 4B, and 5A. However, numerous chromosomal rearrangements reported in early studies could not be confirmed. The genes flanking the breakpoints reported in this study are valuable for isolating these breakpoints.
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Shang
authors.initials: S
authors.lastname: Gao
authors.affiliation: b CSIRO Agriculture Flagship, 306 Carmody Road, St Lucia, QLD 4067, Australia.
authors.forename: Jiri
authors.initials: J
authors.lastname: Stiller
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Qian-Tao
authors.initials: QT
authors.lastname: Jiang
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Xiu-Jin
authors.initials: XJ
authors.lastname: Lan
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Ya-Xi
authors.initials: YX
authors.lastname: Liu
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Zhi-En
authors.initials: ZE
authors.lastname: Pu
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Jirui
authors.initials: J
authors.lastname: Wang
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wei
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.
authors.forename: You-Liang
authors.initials: YL
authors.lastname: Zheng
mesh_terms: D058431:Brachypodium / Q000235:genetics; D056905:Chromosome Breakpoints; D007446:Chromosome Inversion; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D017343:Genes, Plant; D018745:Genome, Plant; D005838:Genotype; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*
keywords: Brachypodium; blé tendre; bread wheat; genome evolution; inversion; jonction de translocation; translocation breakpoint; évolution du génome