title: Microbial Attachment Inhibition through Low-Voltage Electrochemical Reactions on Electrically Conducting Membranes.
abstract: Bacterial biofilm formation on membrane surfaces remains a serious challenge in water treatment systems. The impact of low voltages on microbial attachment to electrically conducting ultrafiltration membranes was investigated using a direct observation cross-flow membrane system mounted on a fluorescence microscope. Escherichia coli and microparticle deposition and detachment rates were measured as a function of the applied electrical potential to the membrane surface. Selecting bacteria and particles with low surface charge minimized electrostatic interactions between the bacteria and charged membrane surface. Application of an electrical potential had a significant impact on the detachment of live bacteria in comparison to dead bacteria and particles. Image analysis indicated that when a potential of 1.5 V was applied to the membrane/counter electrode pair, the percent of dead bacteria was 32±2.1 and 67±3.6% when the membrane was used as a cathode or anode, respectively, while at a potential of 1 V, 92±2.4% were alive. The application of low electrical potentials resulted in the production of low (μM) concentrations of hydrogen peroxide (HP) through the electroreduction of oxygen. The electrochemically produced HP reduced microbial cell viability and increased cellular permeability. Exposure to low concentrations of electrochemically produced HP on the membrane surface prevents bacterial attachment, thus ensuring biofilm-free conditions during membrane filtration operations.
authors.affiliation: Department of Chemical and Environmental Engineering, University of California , Riverside, California 92521, United States.
authors.forename: Avner
authors.initials: A
authors.lastname: Ronen
authors.affiliation: Department of Chemical and Environmental Engineering, University of California , Riverside, California 92521, United States.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Duan
authors.affiliation: Department of Chemical and Environmental Engineering, University of California , Riverside, California 92521, United States.
authors.forename: Ian
authors.initials: I
authors.lastname: Wheeldon
authors.affiliation: Department of Chemical and Environmental Engineering, University of California , Riverside, California 92521, United States.
authors.forename: Sharon
authors.initials: S
authors.lastname: Walker
authors.affiliation: Department of Chemical and Environmental Engineering, University of California , Riverside, California 92521, United States.
authors.forename: David
authors.initials: D
authors.lastname: Jassby
mesh_terms: D001422:Bacterial Adhesion; D018441:Biofilms; D002463:Cell Membrane Permeability; D004553:Electric Conductivity; D004563:Electrochemistry / Q000379:methods*; D004926:Escherichia coli / Q000502:physiology; D005374:Filtration; D007700:Kinetics; D008567:Membranes, Artificial; D050296:Microbial Viability; D008855:Microscopy, Electron, Scanning; D055672:Static Electricity
chemical_list: D008567:Membranes, Artificial