title: Effects of heat stress on peripheral T and B lymphocyte profiles and IgG and IgM serum levels in broiler chickens vaccinated for Newcastle disease virus.
abstract: Multiple factors, such as environment, nutritional status, and disease, induce stress in animals during livestock production. It has been shown that poultry exposed to stressors for prolonged periods had decreases in their performance parameters, mortality and decreased host resistance to pathogenic agents. It seems that early age stress may have long-lasting impact and could possibly modify the expression of their genetic potential on growth performance and immunity. This study aimed to discuss the effects of early-age heat stress on the blood lymphocyte phenotypes (B and T lymphocytes) and plasma immunoglobulin levels (IgM and IgG) in chickens vaccinated against paramixovirus of the Newcastle (NC) disease (LaSota strain). For this purpose, 96 male chickens (Cobb) were divided into 4 groups: 1) control (C), 2) heat-stressed (HS), 3) control vaccinated (C/V), and 4) heat-stressed and Vaccinated (HS/V). The NC vaccine was administered twice on experimental day (ED) 7 and ED14, and the heat stress (38 ± 1°C) was applied from ED2 to ED6. The data showed that HS increased the corticosterone serum levels in the HS group compared with the control groups (C and C/V groups). At ED7, increased concentrations of IgM were observed in birds in the HS and HS/V groups compared with C and C/V animals; chickens from the HS/V group presented increased IgG levels compared with those in the birds of the C group. The heat stress shifted the immune cell profile from B-lymphocyte to a T-cytotoxic and T-helper lymphocyte profile, and this immune cell pattern persisted until the end of the study period. It was concluded that heat stress immunomodulated the immune function response of the chickens to the NC disease vaccine challenge.
authors.affiliation: Neuroimmunomodulation Research Group, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-900, Brazil.
authors.forename: Bruno Takashi Bueno
authors.initials: BT
authors.lastname: Honda
authors.affiliation: Neuroimmunomodulation Research Group, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-900, Brazil.
authors.forename: Atilio Sersun
authors.initials: AS
authors.lastname: Calefi
authors.affiliation: Neuroimmunomodulation Research Group, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-900, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Costola-de-Souza
authors.affiliation: Neuroimmunomodulation Research Group, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-900, Brazil.
authors.forename: Wanderley Moreno
authors.initials: WM
authors.lastname: Quinteiro-Filho
authors.affiliation: Neuroimmunomodulation Research Group, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-900, Brazil.
authors.forename: Juliana Garcia
authors.initials: JG
authors.lastname: da Silva Fonseca
authors.affiliation: Neuroimmunomodulation Research Group, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-900, Brazil.
authors.forename: Viviane Ferraz
authors.initials: VF
authors.lastname: de Paula
authors.affiliation: Neuroimmunomodulation Research Group, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-900, Brazil jpalermo@usp.br.
authors.forename: João
authors.initials: J
authors.lastname: Palermo-Neto
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D002645:Chickens / Q000502:physiology*; D003345:Corticosterone / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D018869:Heat-Shock Response; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D016130:Immunophenotyping / Q000662:veterinary; D008297:Male; D009521:Newcastle Disease / Q000517:prevention & control*; D011897:Random Allocation; D013601:T-Lymphocytes / Q000166:cytology; D014611:Vaccination / Q000662:veterinary*
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003345:Corticosterone
keywords: Newcastle disease; heat stress; immunoglobulins; lymphocytes; neuroimmunomodulation