title: Nondenaturing polyacrylamide gel electrophoresis to study the dissociation of the p53·MDM2/X complex by potentially anticancer compounds.
abstract: A new analytical method to study the dissociation of the complexes between the oncosuppressor p53 and its negative modulators murine double-minute protein 2 (MDM2) or MDMX, is proposed. This technique is reliable to determine the dissociative power exerted by small molecules on the complex taking advantage of the appearance of migrating MDM2 or MDMX in a native polyacrylamide gel, when inhibitors are added to the complex mixture. Therefore, we propose this new approach to easily screen library of compounds, with potential pharmacological anticancer activity.
authors.affiliation: Department of Movement Sciences and Wellness, University of Naples "Parthenope", Naples, Italy.|CEINGE - Biotecnologie Avanzate s.c. a r.l, Naples, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Sgammato
authors.affiliation: Department of Biosciences and Territory, University of Molise, Pesche, Isernia, Italy.
authors.forename: Doriana
authors.initials: D
authors.lastname: Desiderio
authors.affiliation: Department of Movement Sciences and Wellness, University of Naples "Parthenope", Naples, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Lamberti
authors.affiliation: Department of Biosciences and Territory, University of Molise, Pesche, Isernia, Italy.
authors.forename: Gennaro
authors.initials: G
authors.lastname: Raimo
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Naples, Italy.
authors.forename: Ettore
authors.initials: E
authors.lastname: Novellino
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Naples, Italy.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Carotenuto
authors.affiliation: Department of Movement Sciences and Wellness, University of Naples "Parthenope", Naples, Italy.|CEINGE - Biotecnologie Avanzate s.c. a r.l, Naples, Italy.
authors.forename: Mariorosario
authors.initials: M
authors.lastname: Masullo
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D046912:Multiprotein Complexes / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D063347:Native Polyacrylamide Gel Electrophoresis / Q000379:methods*; D051736:Proto-Oncogene Proteins c-mdm2 / Q000737:chemistry* / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D046912:Multiprotein Complexes; D016159:Tumor Suppressor Protein p53; D051736:Proto-Oncogene Proteins c-mdm2
keywords: Complex dissociation; Inhibitors; MDM2; MDMX; p53