title: The protective effects of tadalafil on renal damage following ischemia reperfusion injury in rats.
abstract: Ischemia-reperfusion injury can cause renal damage, and phosphodiesterase inhibitors are reported to regulate antioxidant activity. We investigated the prevention of renal damage using tadalafil after renal ischemia reperfusion (I/R) injury in rats. A total of 21 adult male Wistar albino rats were randomly divided into three groups of seven, including Group 1-control, Group 2-I/R, and Group 3-tadalafil + I/R group (I/R-T group) received tadalafil intraperitoneally at 30 minutes before ischemia. Inducible nitric oxide synthase, endothelial nitric oxide synthase, malondialdehyde, and total antioxidant capacity levels were evaluated, and histopathological changes and apoptosis in the groups were examined. Tadalafil decreased malondialdehyde levels in the I/R group and increased the total antioxidant capacity level. Histopathological and immunohistochemical findings revealed that tadalafil decreased renal injury scores and the ratios of injured cells, as measured through apoptotic protease activating factor 1, inducible nitric oxide synthase, and endothelial nitric oxide synthase levels. We suggest that tadalafil has protective effects against I/R-related renal tissue injury.
authors.affiliation: Department of Urology, Faculty of Medicine, Istanbul Medeniyet University, Istanbul, Turkey. Electronic address: erolbulent@yahoo.com.
authors.forename: Bulent
authors.initials: B
authors.lastname: Erol
authors.affiliation: Department of Urology, Faculty of Medicine, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Tugrul
authors.initials: T
authors.lastname: Turker
authors.affiliation: Department of Urology, Faculty of Medicine, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Adem
authors.initials: A
authors.lastname: Tok
authors.affiliation: Department of Pathology, Faculty of Medicine, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Sibel
authors.initials: S
authors.lastname: Bektas
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Gorkem
authors.initials: G
authors.lastname: Mungan
authors.affiliation: Department of Pathology, Istanbul Medeniyet University, Medical Faculty, Istanbul, Turkey.
authors.forename: Seyma
authors.initials: S
authors.lastname: Ozkanli
authors.affiliation: Department of Urology, Faculty of Medicine, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Bugra
authors.initials: B
authors.lastname: Karakas
authors.affiliation: Department of Urology, Faculty of Medicine, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Husnu
authors.initials: H
authors.lastname: Tokgoz
authors.affiliation: Department of Urology, Faculty of Medicine, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Bulent
authors.initials: B
authors.lastname: Akduman
authors.affiliation: Department of Urology, Faculty of Medicine, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Aydin
authors.initials: A
authors.lastname: Mungan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D053477:Apoptotic Protease-Activating Factor 1 / Q000378:metabolism; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects / Q000473:pathology*; D007684:Kidney Tubules / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008315:Malondialdehyde / Q000097:blood; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D000068581:Tadalafil
chemical_list: D000975:Antioxidants; C506594:Apaf1 protein, rat; D053477:Apoptotic Protease-Activating Factor 1; D020011:Protective Agents; D008315:Malondialdehyde; D000068581:Tadalafil; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III
keywords: Ischemia reperfusion; Kidney; Tadalafil