title: 1H, 15N and 13C resonance assignments of light organ-associated fatty acid-binding protein of Taiwanese fireflies.
abstract: Fatty acid-binding proteins (FABPs) are a family of proteins that modulate the transfer of various fatty acids in the cytosol and constitute a significant portion in many energy-consuming cells. The ligand binding properties and specific functions of a particular type of FABP seem to be diverse and depend on the respective binding cavity as well as the cell type from which this protein is derived. Previously, a novel FABP (lcFABP; lc: Luciola cerata) was identified in the light organ of Taiwanese fireflies. The lcFABP was proved to possess fatty acids binding capabilities, especially for fatty acids of length C14-C18. However, the structural details are unknown, and the structure-function relationship has remained to be further investigated. In this study, we finished the (1)H, (15)N and (13)C chemical shift assignments of (15)N/(13)C-enriched lcFABP by solution NMR spectroscopy. In addition, the secondary structure distribution was revealed based on the backbone N, H, Cα, Hα, C and side chain Cβ assignments. These results can provide the basis for further structural exploration of lcFABP.
authors.affiliation: Institute of Bioinformatics and Structural Biology, National Tsing Hua University, No. 101, Sec. 2, Kuang Fu Rd, Hsinchu, 30013, Taiwan, ROC.
authors.forename: Kai-Li
authors.initials: KL
authors.lastname: Tseng
authors.affiliation: Institute of Bioinformatics and Structural Biology, National Tsing Hua University, No. 101, Sec. 2, Kuang Fu Rd, Hsinchu, 30013, Taiwan, ROC.
authors.forename: Yi-Zong
authors.initials: YZ
authors.lastname: Lee
authors.affiliation: Institute of Bioinformatics and Structural Biology, National Tsing Hua University, No. 101, Sec. 2, Kuang Fu Rd, Hsinchu, 30013, Taiwan, ROC.
authors.forename: Yun-Ru
authors.initials: YR
authors.lastname: Chen
authors.affiliation: Institute of Bioinformatics and Structural Biology, National Tsing Hua University, No. 101, Sec. 2, Kuang Fu Rd, Hsinchu, 30013, Taiwan, ROC. pclyu@life.nthu.edu.tw.|Department of Medical Sciences, National Tsing Hua University, Hsinchu, Taiwan, ROC. pclyu@life.nthu.edu.tw.
authors.forename: Ping-Chiang
authors.initials: PC
authors.lastname: Lyu
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry*; D048888:Fireflies / Q000378:metabolism*; D008027:Light; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D014316:Tritium
chemical_list: D002247:Carbon Isotopes; D050556:Fatty Acid-Binding Proteins; D009587:Nitrogen Isotopes; D014316:Tritium
keywords: Chemical shift assignments; Light organ; Luciola cerata; lcFABP