title: Gold Nanocluster-Assisted Fluorescent Detection for Hydrogen Peroxide and Cholesterol Based on the Inner Filter Effect of Gold Nanoparticles.
abstract: We developed a simple, sensitive inner filter effect (IFE)-based fluorescent assay for sensing H2O2 and cholesterol. In the process, poly(vinylpyrrolidone)-protected gold nanoparticles (PVP-AuNPs) and fluorescent BSA-protected gold nanoclusters (BSA-AuNCs) were used as an IFE absorber/fluorophore pair. PVP-AuNPs can be a powerful absorber to influence the emission of the fluorophore, BSA-AuNCs, in the IFE-based fluorescent assays. That is due to the high extinction coefficient of AuNPs and the complementary overlap between the surface plasmon resonance (SPR) absorption of PVP-AuNPs and the excitation of BSA-AuNCs. The PVP-Au seeds, produced by directly mixing PVP with HAuCl4, were able to catalyze H2O2 to enlarge AuNPs. The SPR absorption of PVP-AuNPs was enhanced with an increased concentration of H2O2 and, subsequently, induced significant fluorescence quenching of BSA-AuNCs. The IFE-based fluorescent assay enabled the detection of H2O2 and generation of H2O2 in the presence of O2/cholesterol and cholesterol oxidase (ChOx) by the fluorescence response of BSA-AuNCs. The present IFE-based approach can detect H2O2 ranging from 1 to 100 μM with a detection limit of 0.8 μM and cholesterol ranging from 1 to 100 μM with a detection limit of 1.4 μM.
authors.affiliation: Department of Chemistry, National Tsing Hua University , No. 101, Section 2, Kuang-Fu Road, Hsinchu 30013, Taiwan.
authors.forename: Heng-Chia
authors.initials: HC
authors.lastname: Chang
authors.affiliation: Department of Chemistry, National Tsing Hua University , No. 101, Section 2, Kuang-Fu Road, Hsinchu 30013, Taiwan.|BioAnalytical Chemistry and Nanobiomedicine Laboratory, Department of Biochemical Science and Technology, National Taiwan University , No. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
authors.forename: Ja-an Annie
authors.initials: JA
authors.lastname: Ho
mesh_terms: D000818:Animals; D002417:Cattle; D002784:Cholesterol / Q000032:analysis*; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D012710:Serum Albumin, Bovine; D006046:Gold; D002784:Cholesterol; D006861:Hydrogen Peroxide