title: Effect of hydroalcoholic leaves extract of Indigofera spicata Forssk. on blood glucose level of normal, glucose loaded and diabetic rodents.
abstract: BACKGROUND
  Diabetes mellitus is found in all parts of the world and is rapidly increasing in its coverage with alarming rate especially in Asia and Africa. Research is increasingly done with the aim of developing a relatively safe and efficacious anti-diabetic plant based products. Parallelly, this investigation was carried out to evaluate the effect of the hydro alcoholic leaves crude extract of Indigofera spicata (ISP) on the blood glucose level(BGL) of normoglycemic, oral glucose loaded and alloxan induced diabetic rodents.
  
  
  METHODS
  The animals were randomly divided into five groups (n = 6) for all the aforementioned three models. In all models, group-I mice provided 2%tween-80, group-II were treated with 5 mg/kg glibenclamide and the remaining three groups (III, IV & V) were treated with 100, 200, and 400 mg/kg dose of the extract respectively. Statistical significance of differences in BGLs within and between groups was analyzed by SPSS version-21 using one way ANOVA followed by Tukey's post hoc multiple comparison.
  
  
  RESULTS
  200 mg/kg and 400 mg/kg extract treated groups of normoglycemic mice showed significant (p < 0.05) BGL reduction compared to the pre-exposure level. In case of OGTT model BGL reduction was statistically significant (p < 0.05) in only 400 mg/kg exposed groups at the 120 min of post-exposure compared to the initial level. However, the BGL reducing effect of doses of the extract at the 4(th), 6(th) and 10(th) hours of post treatment on diabetic mice was found statistically significant compared to both the negative control (p < 0.001) and their respective pretreatment levels (p < 0.05).
  
  
  CONCLUSIONS
  As it is claimed in ethnobotanical studies, the hydroalcoholic crude extract of ISP leaves have shown prominent anti-diabetic effect and can be therefore used as a good insight for new anti-diabetic drug source with a call for further studies.
authors.affiliation: Department of Pharmacology, College of medicine and health sciences, University of Gondar, Gondar, Ethiopia. meshetie21@gmail.com.
authors.forename: Eshetie Melese
authors.initials: EM
authors.lastname: Birru
authors.affiliation: Department of Pharmacology, College of medicine and health sciences, University of Gondar, Gondar, Ethiopia. mohammedB18@gmail.com.
authors.forename: Mohammedbrhan
authors.initials: M
authors.lastname: Abdelwuhab
authors.affiliation: Department of Pharmacology, College of medicine and health sciences, University of Gondar, Gondar, Ethiopia. zeedshow@gmail.com.
authors.forename: Zewdneh
authors.initials: Z
authors.lastname: Shewamene
mesh_terms: D000496:Alloxan; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005905:Glyburide / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D029883:Indigofera / Q000737:chemistry*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D000496:Alloxan; D005905:Glyburide