title: Whole Genome Amplification from Blood Spot Samples.
abstract: Whole genome amplification is an invaluable technique when working with DNA extracted from blood spots, as the DNA obtained from this source often is too limited for extensive genetic analysis. Two techniques that amplify the entire genome are common. Here, both are described with focus on the benefits and drawbacks of each system. However, in order to obtain the best possible WGA result the quality of input DNA extracted from the blood spot is essential, but also time consumption, flexibility in format and elution volume and price of the technology are factors influencing system choice. Here, three DNA extraction techniques are described and the above aspects are compared between the systems.
authors.affiliation: The Danish National Biobank, Statens Serum Institut, Artillerivej 5, Copenhagen, 2300, Danmark. kms@ssi.dk.
authors.forename: Karina Meden
authors.initials: KM
authors.lastname: Sørensen
mesh_terms: D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D059018:DNA Contamination; D053938:DNA Fragmentation; D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D011786:Quality Control; D059010:Single-Cell Analysis / Q000379:methods
chemical_list: D004247:DNA
keywords: Blood spot; Concentration determination; DNA extraction; Fragmentation library; Fragmented DNA; Multiple displacement amplification; Phi29 polymerase; Whole genome amplification