title: De novo peroxisome biogenesis: Evolving concepts and conundrums.
abstract: Peroxisomes proliferate by growth and division of pre-existing peroxisomes or could arise de novo. Though the de novo pathway of peroxisome biogenesis is a more recent discovery, several studies have highlighted key mechanistic details of the pathway. The endoplasmic reticulum (ER) is the primary source of lipids and proteins for the newly-formed peroxisomes. More recently, an intricate sorting process functioning at the ER has been proposed, that segregates specific PMPs first to peroxisome-specific ER domains (pER) and then assembles PMPs selectively into distinct pre-peroxisomal vesicles (ppVs) that later fuse to form import-competent peroxisomes. In addition, plausible roles of the three key peroxins Pex3, Pex16 and Pex19, which are also central to the growth and division pathway, have been suggested in the de novo process. In this review, we discuss key developments and highlight the unexplored avenues in de novo peroxisome biogenesis.
authors.affiliation: Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, 9500 Gilman Drive, UC San Diego, La Jolla, CA 92093-0322, USA.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Agrawal
authors.affiliation: Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, 9500 Gilman Drive, UC San Diego, La Jolla, CA 92093-0322, USA. Electronic address: ssubramani@ucsd.edu.
authors.forename: Suresh
authors.initials: S
authors.lastname: Subramani
mesh_terms: D000818:Animals; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000737:chemistry / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001678:Organelle Biogenesis; D000074401:Peroxins; D020675:Peroxisomes / Q000737:chemistry / Q000378:metabolism*; D010944:Plants / Q000737:chemistry / Q000378:metabolism; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D015003:Yeasts / Q000737:chemistry / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D008565:Membrane Proteins; C106718:PEX16 protein, Yarrowia lipolytica; C109779:PEX19 protein, S cerevisiae; C070895:PEX3 protein, S cerevisiae; D000074401:Peroxins; D020033:Protein Isoforms; D029701:Saccharomyces cerevisiae Proteins
keywords: De novo peroxisome biogenesis; Intra-ER sorting, import of PMPs into the ER, growth and division vs de novo peroxisome biogenesis; Pre-peroxisomal vesicles, ppVs; Pre-peroxisomal-ER, pER; Role of endoplasmic reticulum in peroxisome biogenesis, peroxisomal membrane protein biogenesis; Role of the endoplasmic reticulum in yeast, mammals and plants