title: Role of phytochromes A and B in the regulation of cell death and acclimatory responses to UV stress in Arabidopsis thaliana.
abstract: Plants coordinate their responses to various biotic and abiotic stresses in order to optimize their developmental and acclimatory programmes. The ultimate response to an excessive amount of stress is local induction of cell death mechanisms. The death of certain cells can help to maintain tissue homeostasis and enable nutrient remobilization, thus increasing the survival chances of the whole organism in unfavourable environmental conditions. UV radiation is one of the environmental factors that negatively affects the photosynthetic process and triggers cell death. The aim of this work was to evaluate a possible role of the red/far-red light photoreceptors phytochrome A (phyA) and phytochrome B (phyB) and their interrelations during acclimatory responses to UV stress. We showed that UV-C treatment caused a disturbance in photosystem II and a deregulation of photosynthetic pigment content and antioxidant enzymes activities, followed by increased cell mortality rate in phyB and phyAB null mutants. We also propose a regulatory role of phyA and phyB in CO2 assimilation, non-photochemical quenching, reactive oxygen species accumulation and salicylic acid content. Taken together, our results suggest a novel role of phytochromes as putative regulators of cell death and acclimatory responses to UV.
authors.affiliation: Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), Nowoursynowska Street 159, Warsaw, 02-776 Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Rusaczonek
authors.affiliation: Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), Nowoursynowska Street 159, Warsaw, 02-776 Poland Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences (SGGW), Nowoursynowska Street 159, 02-776 Warsaw, Poland.
authors.forename: Weronika
authors.initials: W
authors.lastname: Czarnocka
authors.affiliation: Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), Nowoursynowska Street 159, Warsaw, 02-776 Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Kacprzak
authors.affiliation: Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), Nowoursynowska Street 159, Warsaw, 02-776 Poland.
authors.forename: Damian
authors.initials: D
authors.lastname: Witoń
authors.affiliation: Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), Nowoursynowska Street 159, Warsaw, 02-776 Poland The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek Street 21, 30-239 Krakow, Poland.
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Ślesak
authors.affiliation: Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), Nowoursynowska Street 159, Warsaw, 02-776 Poland The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek Street 21, 30-239 Krakow, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Szechyńska-Hebda
authors.affiliation: Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), Nowoursynowska Street 159, Warsaw, 02-776 Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Gawroński
authors.affiliation: Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), Nowoursynowska Street 159, Warsaw, 02-776 Poland stanislaw_karpinski@sggw.pl.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Karpiński
mesh_terms: D000064:Acclimatization; D000975:Antioxidants / Q000378:metabolism; D017360:Arabidopsis / Q000502:physiology* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D016923:Cell Death; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D010788:Photosynthesis; D051942:Phytochrome A / Q000235:genetics* / Q000378:metabolism; D051939:Phytochrome B / Q000235:genetics* / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D029681:Arabidopsis Proteins; C497973:PHYA protein, Arabidopsis; C497952:PHYB protein, Arabidopsis; D051942:Phytochrome A; D051939:Phytochrome B
keywords: Antioxidant enzymes; UV stress.; cell death; photosynthesis; phytochromes; reactive oxygen species