title: Dynamics of IL-4 and IL-13 expression in the airways of sheep following allergen challenge.
abstract: BACKGROUND
  IL-4 and IL-13 play a critical yet poorly understood role in orchestrating the recruitment and activation of effector cells of the asthmatic response and driving the pathophysiology of allergic asthma. The house dust mite (HDM) sheep asthma model displays many features of the human condition and is an ideal model to further elucidate the involvement of these critical Th2 cytokines. We hypothesized that airway exposure to HDM allergen would induce or elevate the expression profile of IL-4 and IL-13 during the allergic airway response in this large animal model of asthma.
  
  
  METHODS
  Bronchoalveolar lavage (BAL) samples were collected from saline- and house dust mite (HDM)- challenged lung lobes of sensitized sheep from 0 to 48 h post-challenge. BAL cytokines (IL-4, IL-13, IL-6, IL-10, TNF-α) were each measured by ELISA. IL-4 and IL-13 expression was assessed in BAL leukocytes by flow cytometry and in airway tissue sections by immunohistology.
  
  
  RESULTS
  IL-4 and IL-13 were increased in BAL samples following airway allergen challenge. HDM challenge resulted in a significant increase in BAL IL-4 levels at 4 h compared to saline-challenged airways, while BAL IL-13 levels were elevated at all time-points after allergen challenge. IL-6 levels were maintained following HDM challenge but declined after saline challenge, while HDM administration resulted in an acute elevation in IL-10 at 4 h but no change in TNF-α levels over time. Lymphocytes were the main early source of IL-4, with IL-4 release by alveolar macrophages (AMs) prominent from 24 h post-allergen challenge. IL-13 producing AMs were increased at 4 and 24 h following HDM compared to saline challenge, and tissue staining provided evidence of IL-13 expression in airway epithelium as well as immune cells in airway tissue.
  
  
  CONCLUSIONS
  In a sheep model of allergic asthma, airway inflammation is accompanied by the temporal release of key cytokines following allergen exposure that primarily reflects the Th2-driven nature of the immune response in asthma. The present study demonstrates for the first time the involvement of IL-4 and IL-13 in a relevant large animal model of allergic airways disease.
authors.affiliation: Biotechnology Research Laboratories, Department of Physiology, Monash University, Clayton, 3800, VIC, Australia. bahar.k.liravi@monash.edu.
authors.forename: Bahar
authors.initials: B
authors.lastname: Liravi
authors.affiliation: School of Applied and Biomedical Sciences, Federation University, Churchill, 3842, VIC, Australia. david.piedrafita@federation.edu.au.
authors.forename: David
authors.initials: D
authors.lastname: Piedrafita
authors.affiliation: Biotechnology Research Laboratories, Department of Physiology, Monash University, Clayton, 3800, VIC, Australia. gary.nguyen@monash.edu.
authors.forename: Gary
authors.initials: G
authors.lastname: Nguyen
authors.affiliation: Biotechnology Research Laboratories, Department of Physiology, Monash University, Clayton, 3800, VIC, Australia. rob.bischof@hudson.org.au.|The Ritchie Centre, Hudson Institute of Medical Research, Clayton, 3168, VIC, Australia. rob.bischof@hudson.org.au.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Bischof
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D001249:Asthma / Q000276:immunology*; D016535:Bronchial Hyperreactivity / Q000503:physiopathology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D004195:Disease Models, Animal; D005260:Female; D018793:Interleukin-13 / Q000032:analysis*; D015847:Interleukin-4 / Q000032:analysis*; D039981:Pyroglyphidae / Q000276:immunology*; D012756:Sheep; D058408:Th1-Th2 Balance
chemical_list: D000485:Allergens; D018793:Interleukin-13; D015847:Interleukin-4