title: Vacuum-powered bubble-assisted solvent extraction followed by macroporous resin enrichment for isolation of podophyllotoxin from Sinopodophyllum emodi.
abstract: A vacuum-powered bubble-assisted solvent extraction (VBE) technique was used to extract podophyllotoxin from the root of Sinopodophyllum emodi. We optimized the VBE procedure and showed it had the highest efficiency of extraction compared to other conventional extraction techniques. Based upon the results of single-factor experiments, a three-factor, three-level experiment design was developed by application of a Box-Behnken design. The method was validated by stability, repeatability and recovery experiments. The optimal conditions were: solvent, 60% (v/v) ethanol; particle size of the sample, 60-80 mesh; soak time, 2h; liquid/solid ratio, 21L/kg; air flow, 32mL/min; vacuum-powered bubble extraction time, 65min. The VBE method we developed achieved efficient extraction of podophyllotoxin from S. emodi. The podophyllotoxin extracted can be enriched and separated by an HPD300 macroporous resin adsorption and desorption process. The results indicated that VBE is a convenient, rapid and efficient sample preparation technique.
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Key Laboratory of Forest Plant Ecology, Northeast Forestry University, 150040 Harbin, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China. Electronic address: suixiaoyu@outlook.com.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Sui
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Fu
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 161006 Qiqihar, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liang
mesh_terms: D011034:Podophyllotoxin / Q000302:isolation & purification*; D011035:Podophyllum / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry*; D014618:Vacuum
chemical_list: D012997:Solvents; D011034:Podophyllotoxin
keywords: Macroporous resin; Podophyllotoxin; Response surface methodology; Sinopodophyllum emodi; Vacuum-powered bubble-assisted solvent extraction