title: Caffeic Acid Phenethyl Ester inhibit Hepatic Fibrosis by Nitric Oxide Synthase and Cystathionine Gamma-Lyase in Rats.
abstract: BACKGROUND
  Our aim was to study the effect of caffeic acid phenethyl ester (CAPE) on iNOS and cystathionine gamma-lyase (CSE) of hepatic fibrosis rat, and discuss the anti-hepatic fibrosis mechanism of caffeic acid phenethyl ester.
  
  
  METHODS
  We observed changes of NO and H2S in serum of hepatic fibrosis rats. Enzyme-linked immunosorbent assay was used to test OD value of iNOS and CSE in serum of each. The expressions of iNOS and CSE protein in the liver were also detected by immunohistochemistry.
  
  
  RESULTS
  Compared with the model group, the expression of NO and iNOS was decreased obviously and the level of H2S and CSE was increased in the CAPE group.
  
  
  CONCLUSIONS
  CAPE has the effect of anti-hepatic fibrosis, which can be realized through adjusting the expression level of iNOS and CSE.
authors.affiliation: Department of Histology and Embryology, Binzhou Medical University, Yantai, Shandong, China (mainland).
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Physiology, Binzhou Medical University, Yantai, Shandong, China (mainland).
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.affiliation: Weihai Wengdeng City Center Hospital, Weihai, Shandong, China (mainland).
authors.forename: Lu
authors.initials: L
authors.lastname: Shi
authors.affiliation: Weihai Wengdeng City Center Hospital, Weihai, Shandong, China (mainland).
authors.forename: Jinyang
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Histology and Embryology, Binzhou Medical University, Yantai, Shandong, China (mainland).
authors.forename: Min
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Histology and Embryology, Binzhou Medical University, Yantai, Shandong, China (mainland).
authors.forename: Yana
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D002109:Caffeic Acids / Q000737:chemistry*; D003542:Cystathionine gamma-Lyase / Q000378:metabolism*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D006862:Hydrogen Sulfide / Q000737:chemistry; D007150:Immunohistochemistry; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008103:Liver Cirrhosis / Q000188:drug therapy*; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002109:Caffeic Acids; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D003542:Cystathionine gamma-Lyase; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol; D006862:Hydrogen Sulfide