title: Mesoporous Silica Nanoparticles Decorated with Carbosilane Dendrons as New Non-viral Oligonucleotide Delivery Carriers.
abstract: A novel nanosystem based on mesoporous silica nanoparticles covered with carbosilane dendrons grafted on the external surface of the nanoparticles is reported. This system is able to transport single-stranded oligonucleotide into cells, avoiding an electrostatic repulsion between the cell membrane and the negatively charged nucleic acids thanks to the cationic charge provided by the dendron coating under physiological conditions. Moreover, the presence of the highly ordered pore network inside the silica matrix would make possible to allocate other therapeutic agents within the mesopores with the aim of achieving a double delivery. First, carbosilane dendrons of second and third generation possessing ammonium or tertiary amine groups as peripheral functional groups were prepared. Hence, different strategies were tested in order to obtain their suitable grafting on the outer surface of the nanoparticles. As nucleic acid model, a single-stranded DNA oligonucleotide tagged with a fluorescent Cy3 moiety was used to evaluate the DNA adsorption capacity. The hybrid material functionalised with the third generation of a neutral dendron showed excellent DNA binding properties. Finally, the cytotoxicity as well as the capability to deliver DNA into cells, was tested in vitro by using a human osteoblast-like cell line, achieving good levels of internalisation of the vector DNA/carbosilane dendron-functionalised material without affecting the cellular viability.
authors.affiliation: Departamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid (Spain).|Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) (Spain).
authors.forename: Ángel
authors.initials: Á
authors.lastname: Martínez
authors.affiliation: Departamento de Química Orgánica y Química Inorgánica, Facultad de Farmacia, Universidad de Alcalá, 28871 Alcalá de Henares (Spain).|Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) (Spain).
authors.forename: Elena
authors.initials: E
authors.lastname: Fuentes-Paniagua
authors.affiliation: Departamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid (Spain).|Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) (Spain).
authors.forename: Alejandro
authors.initials: A
authors.lastname: Baeza
authors.affiliation: Departamento de Química Orgánica y Química Inorgánica, Facultad de Farmacia, Universidad de Alcalá, 28871 Alcalá de Henares (Spain).|Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) (Spain).
authors.forename: Javier
authors.initials: J
authors.lastname: Sánchez-Nieves
authors.affiliation: Departamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid (Spain).|Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) (Spain).
authors.forename: Mónica
authors.initials: M
authors.lastname: Cicuéndez
authors.affiliation: Departamento de Química Orgánica y Química Inorgánica, Facultad de Farmacia, Universidad de Alcalá, 28871 Alcalá de Henares (Spain).|Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) (Spain).
authors.forename: Rafael
authors.initials: R
authors.lastname: Gómez
authors.affiliation: Departamento de Química Orgánica y Química Inorgánica, Facultad de Farmacia, Universidad de Alcalá, 28871 Alcalá de Henares (Spain). javier.delamata@uah.es.|Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) (Spain). javier.delamata@uah.es.
authors.forename: F Javier
authors.initials: FJ
authors.lastname: de la Mata
authors.affiliation: Departamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid (Spain). blancaortiz@ucm.es.|Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) (Spain). blancaortiz@ucm.es.
authors.forename: Blanca
authors.initials: B
authors.lastname: González
authors.affiliation: Departamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid (Spain). vallet@ucm.es.|Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) (Spain). vallet@ucm.es.
authors.forename: María
authors.initials: M
authors.lastname: Vallet-Regí
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry*; D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D007477:Ions / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D053758:Nanoparticles / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry* / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry*; D016062:Porosity; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D014162:Transfection / Q000379:methods
chemical_list: D004277:DNA, Single-Stranded; D050091:Dendrimers; D004337:Drug Carriers; D007477:Ions; D009696:Nucleic Acids; D009841:Oligonucleotides; D012821:Silanes; C504072:carbosilane; D012822:Silicon Dioxide
keywords: dendrimers; gene transfection; mesoporous materials; nanoparticles; non-viral vectors