title: Japanese encephalitis virus replicon-based vaccine expressing enterovirus-71 epitope confers dual protection from lethal challenges.
abstract: BACKGROUND
  To construct safer recombinant flavivirus vaccine, we exploited Japanese encephalitis virus (JEV) replicon-based platform to generate single-round infectious particles (SRIPs) that expressed heterologous neutralizing epitope SP70 derived from enterovirus-71 (EV71). Such pseudo-infectious virus particles, named SRIP-SP70, although are not genuine viable viruses, closely mimic live virus infection to elicit immune responses within one round of viral life cycle.
  
  
  RESULTS
  We found that, besides gaining of full protection to thwart JEV lethal challenge, female outbred ICR mice, when were immunized with SRIP-SP70 by prime-boost protocol, could not only induce SP70-specific and IgG2a predominant antibodies but also provide their newborns certain degree of protection against EV71 lethal challenge.
  
  
  CONCLUSIONS
  Our results therefore exemplify that this vaccination strategy could indeed confer an immunized host a dual protective immunity against subsequent lethal challenge from JEV or EV71.
authors.affiliation: Graduate Institute of Life Sciences, National Defense Medical Center, No. 161 Section 6, Ming Chuan E. Road, Taipei, 114, Taiwan, Republic of China (ROC). wulala902@yahoo.com.tw.
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Huang
authors.affiliation: Department of Microbiology and Immunology, National Defense Medical Center, No. 161 Section 6, Ming Chuan E. Road, Taipei, 114, Taiwan, ROC. jiateh.liao@gmail.com.
authors.forename: Jia-Teh
authors.initials: JT
authors.lastname: Liao
authors.affiliation: Department of Biochemistry, National Defense Medical Center, No. 161 Section 6, Ming Chuan E. Road, Taipei, 114, Taiwan, ROC. yenlichen@mail.ndmctsgh.edu.tw.
authors.forename: Li-Chen
authors.initials: LC
authors.lastname: Yen
authors.affiliation: Department of Microbiology and Immunology, National Defense Medical Center, No. 161 Section 6, Ming Chuan E. Road, Taipei, 114, Taiwan, ROC. cqbbbb@gmail.com.
authors.forename: Yung-Kun
authors.initials: YK
authors.lastname: Chang
authors.affiliation: Institute of Biomedical Sciences, Academia Sinica, No. 128 Section 2, Academia Road Nankang, Taipei, 115, Taiwan, ROC. yll@ibms.sinica.edu.tw.
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Lin
authors.affiliation: Department of Microbiology and Immunology, National Defense Medical Center, No. 161 Section 6, Ming Chuan E. Road, Taipei, 114, Taiwan, ROC. chinglen@gmail.com.|National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, No. 35, Keyan Road, Zhunan, Miaoli County, 35053, Taiwan, ROC. chinglen@gmail.com.
authors.forename: Ching-Len
authors.initials: CL
authors.lastname: Liao
mesh_terms: D000818:Animals; D006224:Cricetinae; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000276:immunology*; D029821:Enterovirus A, Human / Q000235:genetics / Q000276:immunology*; D004769:Enterovirus Infections / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000276:immunology; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D012093:Replicon / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology
chemical_list: D000939:Epitopes; D014765:Viral Vaccines