title: An Electrochemical Impedance Spectroscopy-Based Technique to Identify and Quantify Fermentable Sugars in Pineapple Waste Valorization for Bioethanol Production.
abstract: Electrochemical Impedance Spectroscopy (EIS) has been used to develop a methodology able to identify and quantify fermentable sugars present in the enzymatic hydrolysis phase of second-generation bioethanol production from pineapple waste. Thus, a low-cost non-destructive system consisting of a stainless double needle electrode associated to an electronic equipment that allows the implementation of EIS was developed. In order to validate the system, different concentrations of glucose, fructose and sucrose were added to the pineapple waste and analyzed both individually and in combination. Next, statistical data treatment enabled the design of specific Artificial Neural Networks-based mathematical models for each one of the studied sugars and their respective combinations. The obtained prediction models are robust and reliable and they are considered statistically valid (CCR% > 93.443%). These results allow us to introduce this EIS-based technique as an easy, fast, non-destructive, and in-situ alternative to the traditional laboratory methods for enzymatic hydrolysis monitoring.
authors.affiliation: Instituto de Ingeniería de Alimentos para el Desarrollo (IIAD), Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain. clcodo@upvnet.upv.es.
authors.forename: Claudia
authors.initials: C
authors.lastname: Conesa
authors.affiliation: Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universitat Politècnica de València, Universitat de València, Camí de Vera s/n, 46022 Valencia, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: García-Breijo
authors.affiliation: Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universitat Politècnica de València, Universitat de València, Camí de Vera s/n, 46022 Valencia, Spain. edloe@upvnet.upv.es.
authors.forename: Edwin
authors.initials: E
authors.lastname: Loeff
authors.affiliation: Instituto de Ingeniería de Alimentos para el Desarrollo (IIAD), Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain. lusegil@upvnet.upv.es.
authors.forename: Lucía
authors.initials: L
authors.lastname: Seguí
authors.affiliation: Instituto de Ingeniería de Alimentos para el Desarrollo (IIAD), Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain. pfito@tal.upv.es.
authors.forename: Pedro
authors.initials: P
authors.lastname: Fito
authors.affiliation: Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universitat Politècnica de València, Universitat de València, Camí de Vera s/n, 46022 Valencia, Spain. nilami@iqn.upv.es.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Laguarda-Miró
mesh_terms: D031230:Ananas / Q000737:chemistry*; D056804:Biofuels; D018533:Biomass; D002241:Carbohydrates / Q000032:analysis*; D058266:Dielectric Spectroscopy / Q000379:methods*; D000431:Ethanol / Q000378:metabolism*; D025341:Principal Component Analysis
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D000431:Ethanol
keywords: bioethanol; electrochemical impedance spectroscopy; fermentable sugars; pineapple waste; saccharification