title: Isolation, purification, and identification of antialgal substances in green alga Ulva prolifera for antialgal activity against the common harmful red tide microalgae.
abstract: Ten compounds (1~10) were successfully isolated from green algae Ulva prolifera through the combination of silica gel column chromatography, Sephadex LH-20 column chromatography and repeated preparative thin-layer chromatography. These ten compounds showed antialgal activity against red tide microalgae. Among them, compounds 3, 6, and 7 showed stronger antialgal activity against red tide microalgae. Furthermore, their structure was identified on the basis of spectroscopic data. There are three glycoglycerolipids: 1-O-octadecanoic acid-3-O-β-D-galactopyranosyl glycerol (2), 1-O-palmitoyl-3-O-β-D-galactopyranosyl glycerol (4), and 1-O-palmitoyl-2-O-oleoyl-3-O-β-D-galactopyranosyl glycerol (5); two monoglycerides: glycerol monopalmitate (1), 9-hexadecenoic acid, 2,3-dihydroxypropyl ester (3); two terpenoids: loliolide (6) and lsololiolide (7); one lipid-soluble pigments: zeaxanthin (8); one sterol: cholest-5-en-3-ol (9); and one alkaloid: pyrrolopiperazine-2,5-dione (10). These compounds were isolated from U. prolifera for the first time, and compounds 2, 3, 5, and 8 were isolated from marine macroalgae for the first time.
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang, 222005, China. syy-999@163.com.|Co-innovation Center of Jiangsu Marine Bio-Industry Technology, Lianyungang, 222005, China. syy-999@163.com.
authors.forename: Ying-ying
authors.initials: YY
authors.lastname: Sun
authors.affiliation: Beijing Institute of Medical Device Testing, Beijing, 101111, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang, 222005, China.
authors.forename: Gan-lin
authors.initials: GL
authors.lastname: Guo
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang, 222005, China.
authors.forename: Yin-fang
authors.initials: YF
authors.lastname: Pu
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang, 222005, China.
authors.forename: Bin-lun
authors.initials: BL
authors.lastname: Yan
authors.affiliation: Resources and Environment Science Institute, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Chang-hai
authors.initials: CH
authors.lastname: Wang
mesh_terms: D001688:Biological Products / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D057097:Harmful Algal Bloom / Q000187:drug effects*; D058086:Microalgae / Q000187:drug effects* / Q000254:growth & development; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D044445:Ulva / Q000737:chemistry*
chemical_list: D001688:Biological Products; D010936:Plant Extracts
keywords: Antialgal substances; Identification; Isolation; Purification; Red tide microalgae; Ulva prolifera