title: Effects of endovenous laser ablation on vascular tissue: molecular genetics approach.
abstract: BACKGROUND
  Endovenous laser ablation (EVLA) is a treatment option for lower extremity varicose veins. In the present study, we investigate to the genetic changes and possibility of living tissue in the saphenous vein wall after the EVLA procedure.
  
  
  METHODS
  Eleven saphenous vein grafts were randomized in two groups: (1) 4 cm SVG segments of performed EVLA procedure in study group, (2) 4 cm segments of SVG none performed EVLA procedure in control group. SVG were taken from the remnants of distal saphenous vein grafts prepared for the bypass procedure but not used. SVG was approximately 8 cm in length and was divided into two parts 4 cm in length. One half was exposed to laser energy, while the other half of the same vein graft was untreated as a control. EVLA was performed on complete saphenous veins in the study group. Abnormal genetic changes of the SVG were observed with a Tri-Reagent method and quantified with a Nanodrop™ spectrophotometer.
  
  
  RESULTS
  Histopathological changes indicated that the intima including the endothelium was completely necrotized in the study group. It was observed that intimal thermal-energy-induced injury did not reach the media. Histopathological examination showed that homogenous eosinophilic discoloration and coagulation necrosis characterized the laser related thermal damage as well.
  
  
  CONCLUSIONS
  In this preliminary study, we found that living tissue remained in the SVG wall after application of laser ablation, and we also detected abnormal genetic changes in the study group compared with the control group.
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Pamukkale University Denizli, Turkey.
authors.forename: İhsan
authors.initials: İ
authors.lastname: Alur
authors.affiliation: Department of Medical Biology and Genetics, Faculty of Medicine, Pamukkale University Denizli, Turkey.
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Dodurga
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Pamukkale University Denizli, Turkey.
authors.forename: Tevfik
authors.initials: T
authors.lastname: Güneş
authors.affiliation: Department of Medical Biology and Genetics, Faculty of Medicine, Pamukkale University Denizli, Turkey.
authors.forename: Canan
authors.initials: C
authors.lastname: Eroglu
authors.affiliation: Department of Cardiovascular Surgery, Nigde State Hospital Nigde, Türkiye.
authors.forename: Fırat
authors.initials: F
authors.lastname: Durna
authors.affiliation: Department of Pathology, Faculty of Medicine, Pamukkale University Denizli, Turkey.
authors.forename: Nilay Şen
authors.initials: NŞ
authors.lastname: Türk
authors.affiliation: Department of Anatomy, Faculty of Medicine, Pamukkale University Denizli, Turkey.
authors.forename: Esat
authors.initials: E
authors.lastname: Adıgüzel
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Pamukkale University Denizli, Turkey.
authors.forename: Bilgin
authors.initials: B
authors.lastname: Emrecan
keywords: Endovenous laser ablation; apoptosis; genetic; living tissue