title: Presence of an amino acid residue at position 619 required for the function of YidC in Rhodobacter sphaeroides.
abstract: YidC, the bacterial homologous protein of Oxa1 and Alb3, could insert membrane proteins into the membrane. Rhodobacter sphaeroides is a kind of photobacteria with abundant intracytoplasmic membranes. In this study, the functions of R. sphaeroides YidC and its C-terminus were investigated in the Escherichia coli YidC gene depletion strain FTL10. The results showed that RS_YidC could complement the growth of the strain FTL10, but the RS_YidC last 5 residues (619-623, KKRKP) deletion mutant could not. Interestingly, the site-directed RS_YidC mutants of any one or all of these 5 residues were still active. The deletion mutant of the last 4 residues and even the last 4 residues deletion mutant with substitution of the Ala or Glu for Lys619 still had sufficient activity to complement the growth of the strain FTL10. These results indicated that the length of the C-terminus of Rs_YidC is more important for its function than the amino acid composition or the charges of it, and the presence of an amino acid residue at position 619 is required for Rs_YidC function in E. coli. Our result also suggests that Rs_YidC may function differently as compared to its homologs.
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, China.
authors.forename: Dongqing
authors.initials: D
authors.lastname: Xu
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Ran
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, China. Electronic address: wwwang@njau.edu.cn.
authors.forename: Weiwu
authors.initials: W
authors.lastname: Wang
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012242:Rhodobacter sphaeroides / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; C412443:YIDC protein, E coli
keywords: Amino acid residue 619; C-terminus; Last 5 residues; Rhodobacter sphaeroides; YidC