title: Novel synthesizing method of pH-dependent doxorubicin-loaded anti-CD22-labelled drug delivery nanosystem.
abstract: The objective of this study was to investigate the anticancer efficacy of dimercaptosuccinic acid-modified iron oxide magnetic nanoparticles coloaded with anti-CD22 antibodies and doxorubicin (anti-CD22-MNPs-DOX) on non-Hodgkin's lymphoma cells. The physical properties of anti-CD22-MNPs-DOX were studied and its antitumor effect on Raji cells in vitro was evaluated using the Cell Counting Kit-8 assay. Furthermore, cell apoptosis and intracellular accumulation of doxorubicin were determined by flow cytometry. The results revealed that anti-CD22-MNPs-DOX inhibited the proliferation of Raji cells, significantly increased the uptake of doxorubicin, and induced apoptosis. Therefore, it was concluded that a coloaded antibody and chemotherapeutic drug with magnetic nanoparticles might be an efficient targeted treatment strategy for non-Hodgkin's lymphoma.
authors.affiliation: Department of Hematology/Oncology, Nanjing Children's Hospital, Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Mengjiao
authors.initials: M
authors.lastname: Sun
authors.affiliation: Department of Hematology/Oncology, Nanjing Children's Hospital, Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Hematology/Oncology, Nanjing Children's Hospital, Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Department of Hematology, Zhongda Hospital, Medical School, Southeast University, Nanjing, People's Republic of China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, People's Republic of China.
authors.forename: Lina
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Hematology/Oncology, Nanjing Children's Hospital, Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Fang
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000906:Antibodies / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D001692:Biological Transport; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004337:Drug Carriers; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D058185:Magnetite Nanoparticles; D036103:Nanotechnology; D051918:Sialic Acid Binding Ig-like Lectin 2 / Q000276:immunology / Q000378:metabolism*; D012995:Solubility; D004113:Succimer / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D000903:Antibiotics, Antineoplastic; D000906:Antibodies; C497867:CD22 protein, human; D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D051918:Sialic Acid Binding Ig-like Lectin 2; D004317:Doxorubicin; D004113:Succimer
keywords: anti-CD22 antibody; doxorubicin; drug delivery system; non- Hodgkin lymphoma; target selection