title: Novel workflow for combining Raman spectroscopy and MALDI-MSI for tissue based studies.
abstract: Molecular heterogeneity of cancer is a major obstacle in tumor diagnosis and treatment. To deal with this heterogeneity, a multidisciplinary combination of different analysis techniques is of urgent need because a combination enables the creation of a multimodal image of a tumor. Here, we develop a computational workflow in order to combine matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) imaging and Raman microspectroscopic imaging for tissue based studies. The computational workflow can be used to confirm a spectral histopathology (SHP) based on one technique with another technique. In this contribution, we confirmed a Raman spectroscopic based SHP with MALDI-imaging. Owing to this combination, we could demonstrate, for a larynx carcinoma sample, that tissue types and different metabolic states could be extracted from the Raman spectra. Further investigations with the help of MALDI spectra yield a better characterization of variable epithelial differentiation and a better understanding of ongoing dysplastic alterations.
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, University of Jena, Helmholtzweg 4, 07743, Jena, Germany. thomas.bocklitz@uni-jena.de.
authors.forename: Thomas
authors.identifier: http://orcid.org/0000-0003-2778-6624
authors.initials: T
authors.lastname: Bocklitz
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, University of Jena, Helmholtzweg 4, 07743, Jena, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Bräutigam
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstraße 10, 07743, Jena, Germany.
authors.forename: Annett
authors.initials: A
authors.lastname: Urbanek
authors.affiliation: Department of Otorhinolaryngology, Jena University Hospital, Lessingstraße 2, 07743, Jena, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Hoffmann
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, University of Jena, Helmholtzweg 4, 07743, Jena, Germany.|Department of Otorhinolaryngology, Jena University Hospital, Lessingstraße 2, 07743, Jena, Germany.|Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743, Jena, Germany.|Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745, Jena, Germany.
authors.forename: Ferdinand
authors.initials: F
authors.lastname: von Eggeling
authors.affiliation: Department of Otorhinolaryngology, Jena University Hospital, Lessingstraße 2, 07743, Jena, Germany.
authors.forename: Günther
authors.initials: G
authors.lastname: Ernst
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, University of Jena, Helmholtzweg 4, 07743, Jena, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Schmitt
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstraße 10, 07743, Jena, Germany.|Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743, Jena, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schubert
authors.affiliation: Department of Otorhinolaryngology, Jena University Hospital, Lessingstraße 2, 07743, Jena, Germany.
authors.forename: Orlando
authors.initials: O
authors.lastname: Guntinas-Lichius
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, University of Jena, Helmholtzweg 4, 07743, Jena, Germany. juergen.popp@ipht-jena.de.|Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745, Jena, Germany. juergen.popp@ipht-jena.de.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
mesh_terms: D003952:Diagnostic Imaging / Q000379:methods; D006801:Humans; D007822:Laryngeal Neoplasms / Q000175:diagnosis* / Q000473:pathology; D007830:Larynx / Q000473:pathology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D057188:Workflow
keywords: Cancer; MALDI-MS imaging; Multimodal imaging; Raman spectroscopic imaging