title: Development of a novel protein biochip enabling validation of immunological assays and detection of serum IgG and IgM antibodies against Treponema pallidum pathogens in the patients with syphilis.
abstract: In this study, we developed a novel protein biochip methodology that was characterized by dithiobis (succinimidyl undecanoate) (DSU) and specialized for detection of serum IgG and IgM antibodies against Treponema pallidum pathogens in the patients with syphilis, respectively. The biochips were validated by a dimension of atomic force microscope (AFM). The visualized detection limit of IgG antibody on the biochip was 0.39μg/ml. Finally, 286 serum samples from the patients with syphilis were simultaneously tested on the rTpN15-17-47 coated biochips. The results were evaluated in comparison with the assays of T. pallidum particle agglutination (TPPA) and the toluidine red unheated serum test (TRUST). The result demonstrated that the relative positive rate in the 286 patients by biochip was 99.0%, similar to that by TPPA (97.9%, P>0.05) and higher than that by TRUST, (76.2%, P<0.01). The detection specificities were 100% for the biochip and the TPPA and 97.0% for the TRUST. Thus, the protein biochip would provide a useful platform not only for enabling concurrent detection of the infectious antibodies directed against T. pallidum on a larger scale, but also for monitoring therapy modality of the disease.
authors.affiliation: Department of Biology, School of Life Sciences, Anhui Medical University, Hefei 230032, China.
authors.forename: Na-Li
authors.initials: NL
authors.lastname: Huang
authors.affiliation: Department of Biology, School of Life Sciences, Anhui Medical University, Hefei 230032, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ye
authors.affiliation: Sektion Experimentelle Anaesthesiologie, Universitaetsklinikum Ulm, Ulm 89081, Germany.
authors.forename: Marion E
authors.initials: ME
authors.lastname: Schneider
authors.affiliation: Sektion Experimentelle Anaesthesiologie, Universitaetsklinikum Ulm, Ulm 89081, Germany.
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Du
authors.affiliation: Clinical Laboratory, the First Affiliated Hospital, Anhui Medical University, Hefei 230032, China.
authors.forename: Yuan-Hong
authors.initials: YH
authors.lastname: Xu
authors.affiliation: Department of Biology, School of Life Sciences, Anhui Medical University, Hefei 230032, China.
authors.forename: Li-Bin
authors.initials: LB
authors.lastname: Fan
authors.affiliation: Department of Biology, School of Life Sciences, Anhui Medical University, Hefei 230032, China; Sektion Experimentelle Anaesthesiologie, Universitaetsklinikum Ulm, Ulm 89081, Germany; Department of Pathology, Anhui Medical University, Hefei 230032, China. Electronic address: weidongdu@hotmail.com.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Du
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D015374:Biosensing Techniques; D005260:Female; D006801:Humans; D007118:Immunoassay; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D013388:Succinimides / Q000737:chemistry; D013587:Syphilis / Q000097:blood* / Q000382:microbiology; D014210:Treponema pallidum / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013388:Succinimides
keywords: Biochip; Dithiobis (succinimidyl undecanoate) (DSU); Syphilis; T. pallidum particle agglutination test (TPPA); The toluidine red unheated serum test (TRUST); Treponema pallidum