title: Integration of Shh and Wnt Signaling Pathways Regulating Hematopoiesis.
abstract: To investigate the spatial and temporal programmed expression of Shh and Wnt members during key stages of definitive hematopoiesis and the possible mechanism of Shh and Wnt signaling pathways regulating the proliferation of hematopoietic progenitor cells (HPCs). Spatial and temporal programmed gene expression of Shh and Wnt signaling during hematopoiesis corresponded with c-kit(+)lin(-) HPCs proliferation. C-kit(+)Lin(-) populations derived from aorta-gonad-mesonephros (AGM) of Balb/c mice at E10.5 with increased expression of Shh and Wnt3a demonstrated a greater potential for proliferation. Additionally, supplementation with soluble Shh N-terminal peptide promoted the proliferation of c-kit(+)Lin(-) populations by activating the Wnt signaling pathway, an effect which was inhibited by blocking Shh signaling. A specific inhibitor of wnt signaling was capable of inhibiting Shh-induced proliferation in a similar manner to shh inhibitor. Our results provide valuable information on Shh and Wnt signaling involved in hematopoiesis and highlight the importance of interaction of Shh and Wnt signaling in regulating HPCs proliferation.
authors.affiliation: 1 Department of Intensive Care Unit, Shanghai Jiaotong University Affiliated First People's Hospital , Shanghai, China .
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: 2 Department of Hematology, Shanghai Jiaotong University Affiliated First People's Hospital , Shanghai, China .
authors.forename: Liping
authors.initials: L
authors.lastname: Wan
authors.affiliation: 2 Department of Hematology, Shanghai Jiaotong University Affiliated First People's Hospital , Shanghai, China .
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.affiliation: 2 Department of Hematology, Shanghai Jiaotong University Affiliated First People's Hospital , Shanghai, China .
authors.forename: Kun
authors.initials: K
authors.lastname: Zhou
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000502:physiology; D053823:Hedgehog Proteins / Q000235:genetics / Q000502:physiology*; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D015398:Signal Transduction; D051153:Wnt Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D053823:Hedgehog Proteins; C465570:Shh protein, mouse; D051153:Wnt Proteins