title: ICSH recommendations for assessing automated high-performance liquid chromatography and capillary electrophoresis equipment for the quantitation of HbA2.
abstract: Automated high performance liquid chromatography and Capillary electrophoresis are used to quantitate the proportion of Hemoglobin A2 (HbA2 ) in blood samples order to enable screening and diagnosis of carriers of β-thalassemia. Since there is only a very small difference in HbA2 levels between people who are carriers and people who are not carriers such analyses need to be both precise and accurate. This paper examines the different parameters of such equipment and discusses how they should be assessed.
authors.affiliation: Department of Haematology, University College, London, UK.
authors.forename: A D
authors.initials: AD
authors.lastname: Stephens
authors.affiliation: National Institute of Immunohaematology (ICMR), Mumbai, India.
authors.forename: R
authors.initials: R
authors.lastname: Colah
authors.affiliation: Thalassaemia Research Center, Institute of Medical Biosciences, Mahidol University, Nakornpathom, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Fucharoen
authors.affiliation: Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
authors.forename: J
authors.initials: J
authors.lastname: Hoyer
authors.affiliation: Clinical Pathology Laboratories, The University of Michigan, Ann Arbor, MI, USA.
authors.forename: D
authors.initials: D
authors.lastname: Keren
authors.affiliation: Institute for Quality Management in Healthcare (IQMH), University Avenue, Toronto, ON, Canada.
authors.forename: A
authors.initials: A
authors.lastname: McFarlane
authors.affiliation: Barts & The London School of Medicine Charterhouse Square, London, UK.
authors.forename: D
authors.initials: D
authors.lastname: Perrett
authors.affiliation: Imperial College School of Medicine, Hammersmith Hospital, London, UK.
authors.forename: B J
authors.initials: BJ
authors.lastname: Wild
mesh_terms: D057205:Automation, Laboratory; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods / Q000592:standards; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods / Q000592:standards; D006443:Hemoglobin A2 / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017086:beta-Thalassemia / Q000097:blood / Q000175:diagnosis / Q000235:genetics
chemical_list: D006443:Hemoglobin A2
keywords: High-performance liquid chromatography; capillary electrophoresis; hemoglobin A2; β-thalassemia