title: The bacterial divisome: ready for its close-up.
abstract: Bacterial cells divide by targeting a transmembrane protein machine to the division site and regulating its assembly and disassembly so that cytokinesis occurs at the correct time in the cell cycle. The structure and dynamics of this machine (divisome) in bacterial model systems are coming more clearly into focus, thanks to incisive cell biology methods in combination with biochemical and genetic approaches. The main conserved structural element of the machine is the tubulin homologue FtsZ, which assembles into a circumferential ring at the division site that is stabilized and anchored to the inner surface of the cytoplasmic membrane by FtsZ-binding proteins. Once this ring is in place, it recruits a series of transmembrane proteins that ultimately trigger cytokinesis. This review will survey the methods used to characterize the structure of the bacterial divisome, focusing mainly on the Escherichia coli model system, as well as the challenges that remain. These methods include recent super-resolution microscopy, cryo-electron tomography and synthetic reconstitution.
authors.affiliation: Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, Houston, TX 77030, USA.
authors.forename: Veronica W
authors.initials: VW
authors.lastname: Rowlett
authors.affiliation: Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, Houston, TX 77030, USA william.margolin@uth.tmc.edu.
authors.forename: William
authors.initials: W
authors.lastname: Margolin
mesh_terms: D001419:Bacteria / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002455:Cell Division / Q000235:genetics / Q000502:physiology*; D021961:Cell Membrane Structures / Q000235:genetics / Q000378:metabolism; D048749:Cytokinesis; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D025941:Protein Interaction Mapping
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria
keywords: FtsA; FtsZ; bacteria; cytokinesis; septum