title: Development and validation of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of tigecycline in rat brain tissues.
abstract: Tigecycline (TIG), a derivative of minocycline, is the first in the novel class of glycylcyclines and is currently indicated for the treatment of complicated skin structure and intra-abdominal infections. A selective, accurate and reversed-phase high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the determination of TIG in rat brain tissues. Sample preparation was based on protein precipitation and solid phase extraction using Supel-Select HLB (30 mg/1 mL) cartridges. The samples were separated on a YMC Triart C18 column (150 mm x 3.0 mm. 3.0 µm) using gradient elution. Positive electrospray ionization (ESI+) was used for the detection mechanism with the multiple reaction monitoring (MRM) mode. The method was validated over the concentration range of 150-1200 ng/mL for rat brain tissue. The precision and accuracy for all brain analyses were within the acceptable limit. The mean extraction recovery in rat brain was 83.6%. This validated method was successfully applied to a pharmacokinetic study in female Sprague Dawley rats, which were given a dose of 25 mg/kg TIG intraperitoneally at various time-points. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Catalysis and Peptide Research Unit, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.
authors.forename: Chiedza F
authors.initials: CF
authors.lastname: Munyeza
authors.affiliation: Catalysis and Peptide Research Unit, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.
authors.forename: Adeola
authors.initials: A
authors.lastname: Shobo
authors.affiliation: Catalysis and Peptide Research Unit, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.
authors.forename: Sooraj
authors.initials: S
authors.lastname: Baijnath
authors.affiliation: Catalysis and Peptide Research Unit, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.
authors.forename: Dominika
authors.initials: D
authors.lastname: Bratkowska
authors.affiliation: Catalysis and Peptide Research Unit, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.
authors.forename: Suhashni
authors.initials: S
authors.lastname: Naiker
authors.affiliation: Biomedical Resource Unit, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.
authors.forename: Linda A
authors.initials: LA
authors.lastname: Bester
authors.affiliation: Biomedical Resource Unit, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.
authors.forename: Sanil D
authors.initials: SD
authors.lastname: Singh
authors.affiliation: Catalysis and Peptide Research Unit, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.
authors.forename: Glenn E M
authors.initials: GE
authors.lastname: Maguire
authors.affiliation: Catalysis and Peptide Research Unit, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.
authors.forename: Hendrik G
authors.initials: HG
authors.lastname: Kruger
authors.affiliation: Catalysis and Peptide Research Unit, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.
authors.forename: Tricia
authors.initials: T
authors.lastname: Naicker
authors.affiliation: Catalysis and Peptide Research Unit, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.
authors.forename: Thavendran
authors.initials: T
authors.lastname: Govender
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005260:Female; D057230:Limit of Detection; D008911:Minocycline / Q000031:analogs & derivatives* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000078304:Tigecycline
chemical_list: D000900:Anti-Bacterial Agents; D000078304:Tigecycline; D008911:Minocycline
keywords: LC-MS/MS; rat brain tissues; tigecycline