title: A modified experimental model of malignant pleural disease induced by lung Lewis carcinoma (LLC) cells.
abstract: BACKGROUND
  Malignant pleural effusion resulting mainly from pleural metastases of lung adenocarcinoma has clinical relevance, being a sign of poor prognosis and low life expectancy. Experimental models can mimic the human condition, contributing to advances in current understanding of the mechanisms patients' pleural fluid accumulation and possible therapeutic strategies. The objective of this study is to evaluate the role of different concentrations of Lewis lung carcinoma cells (LLC cells) at the time of induction of experimental MPE and the main effects on survival of animals.
  
  
  METHODS
  C57BL/6 mice received intrapleural injection of 0.1, 0.5 or 1.5 × 10(5) LLC cells and survival curve, biochemical and pathological analyses of pleural fluid and tissue were analyzed.
  
  
  RESULTS
  Evaluation of weight loss, mobility and survival showed that animals that received 0.5 × 10(5) cells maintained more stable condition up to day 14 and a gain of 6 days survival over mice that received the highest concentration.
  
  
  CONCLUSIONS
  This study may allow a better understanding the mechanisms involved in the development of malignant pleural effusion and it may be promising in evaluating therapy to avoid recurrence, as the best time to indicate pleurodesis or target therapies.
authors.affiliation: Pleura Laboratory, Pulmonary Division, Heart Institute (InCor), University of Sao Paulo Medical School, Rua Dr. Eneas de Carvalho Aguiar, 44, Cerqueira César, São Paulo, Zip code: 05403-000, Brazil. milena.acencio@incor.usp.br.
authors.forename: Milena Marques Pagliarelli
authors.initials: MM
authors.lastname: Acencio
authors.affiliation: Pleura Laboratory, Pulmonary Division, Heart Institute (InCor), University of Sao Paulo Medical School, Rua Dr. Eneas de Carvalho Aguiar, 44, Cerqueira César, São Paulo, Zip code: 05403-000, Brazil. jupuka@hotmail.com.
authors.forename: Juliana
authors.initials: J
authors.lastname: Puka
authors.affiliation: Pleura Laboratory, Pulmonary Division, Heart Institute (InCor), University of Sao Paulo Medical School, Rua Dr. Eneas de Carvalho Aguiar, 44, Cerqueira César, São Paulo, Zip code: 05403-000, Brazil. evmarchi@uol.com.br.|Medical College of Jundiai, São Paulo, Brazil. evmarchi@uol.com.br.
authors.forename: Evaldo
authors.initials: E
authors.lastname: Marchi
authors.affiliation: Pleura Laboratory, Pulmonary Division, Heart Institute (InCor), University of Sao Paulo Medical School, Rua Dr. Eneas de Carvalho Aguiar, 44, Cerqueira César, São Paulo, Zip code: 05403-000, Brazil. l.antonangelo@uol.com.br.|Clinical Laboratory and LIM 03, Department of Pathology, Hospital das Clínicas, University of Sao Paulo Medical School, São Paulo, Brazil. l.antonangelo@uol.com.br.
authors.forename: Leila
authors.initials: L
authors.lastname: Antonangelo
authors.affiliation: Division of Thoracic Surgery, Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil. rmterra@uol.com.br.
authors.forename: Ricardo Mingarini
authors.initials: RM
authors.lastname: Terra
authors.affiliation: Pleura Laboratory, Pulmonary Division, Heart Institute (InCor), University of Sao Paulo Medical School, Rua Dr. Eneas de Carvalho Aguiar, 44, Cerqueira César, São Paulo, Zip code: 05403-000, Brazil. fsvargas@gmail.com.
authors.forename: Francisco Suso
authors.initials: FS
authors.lastname: Vargas
authors.affiliation: Pleura Laboratory, Pulmonary Division, Heart Institute (InCor), University of Sao Paulo Medical School, Rua Dr. Eneas de Carvalho Aguiar, 44, Cerqueira César, São Paulo, Zip code: 05403-000, Brazil. vcapelozzi@lim05.fm.usp.br.
authors.forename: Vera Luiza
authors.initials: VL
authors.lastname: Capelozzi
authors.affiliation: Pleura Laboratory, Pulmonary Division, Heart Institute (InCor), University of Sao Paulo Medical School, Rua Dr. Eneas de Carvalho Aguiar, 44, Cerqueira César, São Paulo, Zip code: 05403-000, Brazil. lisetepneumo@yahoo.com.br.
authors.forename: Lisete Ribeiro
authors.initials: LR
authors.lastname: Teixeira
mesh_terms: D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000473:pathology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D004195:Disease Models, Animal; D006801:Humans; D008017:Life Expectancy; D008175:Lung Neoplasms / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation / Q000379:methods*; D016066:Pleural Effusion, Malignant / Q000473:pathology*; D018700:Pleurodesis; D011379:Prognosis