title: miR-203 Acts as a Tumor Suppressor Gene in Osteosarcoma by Regulating RAB22A.
abstract: microRNAs (miRNAs), small noncoding RNAs of 19-25 nt, play an important roles in the pathological processes of tumorigenesis. The object of this study was to study the expression and function of miR-203 and to found its target gene in osteosarcoma. In our study, we found the expression level of miR-203 was significantly downregulated in osteosarcoma cell lines and tissues. In addition, overexpression of miR-203 inhibited the osteosarcoma cell proliferation and migration and inhibited Mesenchymal-to-Epithelial reversion Transition (MErT). Moreover, we identified RAB22A as a direct target of miR-203 and RAB22A overexpression blocks the roles of miR-203 in osteosarcoma cell. Furthermore, we demonstrated that RAB22A expression was upregulated in human osteosarcoma cell lines and tissues. Take together, our results demonstrated that miR-203 act as a tumor suppressor miRNA through regulating RAB22A expression and suggested its involvement in osteosarcoma progression and carcinogenesis.
authors.affiliation: Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, Shan xi, China; Department of Orthopedics, The Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai Tenth People's Hospital Tongji University School of Medicine, Shanghai, 200011, China.
authors.forename: Guangpeng
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, Shan xi, China.
authors.forename: Kunzheng
authors.initials: K
authors.lastname: Wang
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020691:rab GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C546703:MIRN203 microRNA, human; D035683:MicroRNAs; C484191:RAB22A protein, human; D020691:rab GTP-Binding Proteins