title: Influence of cationic lipid concentration on properties of lipid-polymer hybrid nanospheres for gene delivery.
abstract: Nanoparticles have been widely used for nonviral gene delivery. Recently, cationic hybrid nanoparticles consisting of two different materials were suggested as a promising delivery vehicle. In this study, nanospheres with a poly(D,L-lactic-co-glycolic acid) (PLGA) core and cationic lipid shell were prepared, and the effect of cationic lipid concentrations on the properties of lipid polymer hybrid nanocarriers investigated. Lipid-polymer hybrid nanospheres (LPHNSs) were fabricated by the emulsion-solvent evaporation method using different concentrations of cationic lipids and characterized for size, surface charge, stability, plasmid DNA-binding capacity, cytotoxicity, and transfection efficiency. All LPHNSs had narrow size distribution with positive surface charges (ζ-potential 52-60 mV), and showed excellent plasmid DNA-binding capacity. In vitro cytotoxicity measurements with HEK293T, HeLa, HaCaT, and HepG2 cells also showed that LPHNSs exhibited less cytotoxicity than conventional transfection agents, such as Lipofectamine and polyethyleneimine-PLGA. As cationic lipid concentrations increased, the particle size of LPHNSs decreased while their ζ-potential increased. In addition, the in vitro transfection efficiency of LPHNSs increased as lipid concentration increased.
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea ; Department of Integrative Engineering, Chung-Ang University, Seoul, South Korea.
authors.forename: Rajendran J C
authors.initials: RJ
authors.lastname: Bose
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea.
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Arai
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea.
authors.forename: Jong Chan
authors.initials: JC
authors.lastname: Ahn
authors.affiliation: Department of Integrative Engineering, Chung-Ang University, Seoul, South Korea.
authors.forename: Hansoo
authors.initials: H
authors.lastname: Park
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea.
authors.forename: Soo-Hong
authors.initials: SH
authors.lastname: Lee
mesh_terms: D002412:Cations / Q000737:chemistry*; D045744:Cell Line, Tumor; D004655:Emulsions; D018014:Gene Transfer Techniques; D057809:HEK293 Cells; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D054118:Nanospheres / Q000737:chemistry*; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D014162:Transfection
chemical_list: D002412:Cations; D004655:Emulsions; D008055:Lipids; C086724:Lipofectamine; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011094:Polyethyleneimine
keywords: core–shell hybrid nanospheres; lipid concentration; low cytotoxicity; surface modification; transfection efficiency