title: IL-10 Protects Neurites in Oxygen-Glucose-Deprived Cortical Neurons through the PI3K/Akt Pathway.
abstract: IL-10, as a cytokine, has an anti-inflammatory cascade following various injuries, but it remains blurred whether IL-10 protects neurites of cortical neurons after oxygen-glucose deprivation injury. Here, we reported that IL-10, in a concentration-dependent manner, reduced neuronal apoptosis and increased neuronal survival in oxygen-glucose-deprived primary cortical neurons, producing an optimal protective effect at 20ng/ml. After staining NF-H and GAP-43, we found that IL-10 significantly protected neurites in terms of axon length and dendrite number by confocal microscopy. Furthermore, it induced the phosphorylation of AKT, suppressed the activation of caspase-3, and up-regulated the protein expression of GAP-43. In contrast, LY294002, a specific inhibitor of PI3K/AKT, reduced the level of AKT phosphorylation and GAP-43 expression, increased active caspase-3 expression and thus significantly weakened IL-10-mediated protective effect in the OGD-induced injury model. IL-10NA, the IL-10 neutralizing antibody, reduced the level of p-PI3K phosphorylation and increased the expression of active caspase-3. These findings suggest that IL-10 provides neuroprotective effects by protecting neurites through PI3K/AKT signaling pathway in oxygen-glucose-deprived primary cortical neurons.
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China; Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Longzai
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China; Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Chen
authors.affiliation: Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China; Department of Rehabilitation, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.
authors.forename: Yixian
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China; Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China; Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China; Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Tin
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China; Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China; Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Jianhao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China; Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Houwei
authors.initials: H
authors.lastname: Du
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China; Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Chen
authors.affiliation: Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Neurology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China; Institute of Cerebral Vascular Disease of Fujian Province, Fuzhou, Fujian, People's Republic of China; Department of Rehabilitation, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Liu
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D015687:Cell Hypoxia; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism; D019922:GAP-43 Protein / Q000378:metabolism; D005947:Glucose / Q000172:deficiency*; D016753:Interleukin-10 / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015290:Second Messenger Systems
chemical_list: D019922:GAP-43 Protein; D018696:Neuroprotective Agents; D016753:Interleukin-10; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D053148:Caspase 3; D005947:Glucose; D010100:Oxygen