title: Anticoagulant flavonoid oligomers from the rhizomes of Alpinia platychilus.
abstract: Two pairs of enantiomers of flavonoid oligomers (1a and 1b, 2a and 2b) along with one known chalcone (3) were isolated from the rhizomes of Alpinia platychilus. Their structures were elucidated on the basis of spectroscopic data (MS and 1D/2D NMR). The absolute configurations of the flavonoid oligomers were established by their ECD spectra. Separation of the enantiomeric mixtures (1a and 1b, 2a and 2b) was achieved on a chiral column using hexane:isopropyl alcohol:ethanol (7:2:1) as eluents. The anticoagulant assay showed that 2a, 2b and 3 exhibited potent activities to prolong the prothrombin times (PT) and the thrombin times (TT).
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
authors.forename: Chuan-Pu
authors.initials: CP
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
authors.forename: Jian-Guang
authors.initials: JG
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China. Electronic address: cpu_lykong@126.com.
authors.forename: Ling-Yi
authors.initials: LY
authors.lastname: Kong
mesh_terms: D032429:Alpinia / Q000737:chemistry*; D000818:Animals; D000925:Anticoagulants / Q000737:chemistry* / Q000302:isolation & purification; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D008297:Male; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D011517:Prothrombin Time; D011817:Rabbits; D027343:Rhizome / Q000737:chemistry; D013918:Thrombin Time
chemical_list: D000925:Anticoagulants; D005419:Flavonoids; D010936:Plant Extracts
keywords: Alpinia platychilus; Anticoagulation; DiChloromethane (PubChem CID: 6344); Dimethyl sulfoxide (DMSO) (PubChem CID: 679); Ethanol (PubChem CID: 702); Ethyl ethanoate (EtOAc) (PubChem CID: 8857); Flavonoid oligomers; HCl (PubChem CID: 313); Isopropyl (PubChem CID: 3776); Methanol (PubChem CID: 887); N-Hexane (PubChem CID: 8058); Sodium citrate (PubChem CID: 6224); Tetramethylsilane (TMS) (PubChem CID: 6396)