title: Investigation of Rho-Kinase Expressions and Polymorphisms in Mantle Cell Lymphoma Patients.
abstract: OBJECTIVE
  Mantle cell lymphoma (MCL) is a rare but aggressive form of B-cell non-Hodgkin lymphoma characterized by excessive expression of cyclin D1. Intracellular signaling enzyme Rho-kinase (ROCK) can contribute to cellular migration, proliferation, and differentiation, as well as tumor development and metastasis. However, ROCK gene and protein expressions or polymorphisms have never been investigated in MCL patients. The purpose of this study was to investigate the role of ROCK gene and protein expressions in MCL patients. We also examined ROCK2 gene polymorphisms in this study.
  
  
  METHODS
  A total of 60 patients with MCL and 60 healthy controls were included in this retrospective study. Hematoxylin and eosin-stained lymph node tissue slides in the entire archive were reevaluated and used for immunohistochemistry, gene expression, and polymerase chain reaction studies.
  
  
  RESULTS
  In immunohistochemical studies, there were significant increases in ROCK1 (p=0.0009) and ROCK2 (p<0.0001) protein expressions in MCL patients when compared with the control group. Although a marked increase in ROCK1 gene expression (p=0.0215) was noted, no significant change was observed in ROCK2 gene expression in MCL patients. Seven ROCK2 polymorphisms were studied, but the results showed no significant differences between the groups.
  
  
  CONCLUSIONS
  This is the first study to show that ROCK1 gene and ROCK protein expressions may contribute to the development of MCL.
authors.affiliation: Gaziantep University Faculty of Medicine, Department of Internal Medicine, Division of Hematology, Gaziantep, Turkey, Phone : +90 532 157 76 56, E-mail : didaryanardag@gmail.com.
authors.forename: Didar
authors.initials: D
authors.lastname: Yanardağ Açık
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yılmaz
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Sarı
authors.forename: Serdar
authors.initials: S
authors.lastname: Öztuzcu
authors.forename: Zeynel A
authors.initials: ZA
authors.lastname: Sayıner
authors.forename: Salih
authors.initials: S
authors.lastname: Subari
authors.forename: Abdullah T
authors.initials: AT
authors.lastname: Demiryürek
mesh_terms: D000368:Aged; D000483:Alleles; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007150:Immunohistochemistry; D020522:Lymphoma, Mantle-Cell / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011110:Polymorphism, Genetic; D011379:Prognosis; D016896:Treatment Outcome; D054460:rho-Associated Kinases / Q000235:genetics* / Q000378:metabolism
chemical_list: D054460:rho-Associated Kinases