title: Impact of platelet rich plasma and adipose stem cells on lymphangiogenesis in a murine tail lymphedema model.
abstract: BACKGROUND
  Lymphedema is an underdiagnosed pathology which in industrialized countries mainly affects cancer patients that underwent lymph node dissection and/or radiation. Currently no effective therapy is available so that patients' life quality is compromised by swellings of the concerned body region. This unfortunate condition is associated with body imbalance and subsequent osteochondral deformations and impaired function as well as with an increased risk of potentially life threatening soft tissue infections.
  
  
  METHODS
  The effects of PRP and ASC on angiogenesis (anti-CD31 staining), microcirculation (Laser Doppler Imaging), lymphangiogenesis (anti-LYVE1 staining), microvascular architecture (corrosion casting) and wound healing (digital planimetry) are studied in a murine tail lymphedema model.
  
  
  RESULTS
  Wounds treated by PRP and ASC healed faster and showed a significantly increased epithelialization mainly from the proximal wound margin. The application of PRP induced a significantly increased lymphangiogenesis while the application of ASC did not induce any significant change in this regard.
  
  
  CONCLUSIONS
  PRP and ASC affect lymphangiogenesis and lymphedema development and might represent a promising approach to improve regeneration of lymphatic vessels, restore disrupted lymphatic circulation and treat or prevent lymphedema alone or in combination with currently available lymphedema therapies.
authors.affiliation: Institute of Functional and Clinical Anatomy, Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Ackermann
authors.affiliation: Department of Plastic, Reconstructive and Aesthetic Surgery, University Hospital of Lausanne (CHUV), Lausanne, Switzerland; Department of Plastic, Reconstructive and Aesthetic Surgery, University Hospital of Basel, Basel, Switzerland.
authors.forename: Reto
authors.initials: R
authors.lastname: Wettstein
authors.affiliation: Department of Plastic, Reconstructive and Aesthetic Surgery, University Hospital of Lausanne (CHUV), Lausanne, Switzerland.
authors.forename: Christopher
authors.initials: C
authors.lastname: Senaldi
authors.affiliation: Department of Plastic, Reconstructive and Aesthetic Surgery, University Hospital of Lausanne (CHUV), Lausanne, Switzerland; Department of Plastic, Reconstructive and Aesthetic Surgery, University Hospital of Basel, Basel, Switzerland.
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Kalbermatten
authors.affiliation: Institute of Functional and Clinical Anatomy, Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Moritz A
authors.initials: MA
authors.lastname: Konerding
authors.affiliation: Department of Plastic, Reconstructive and Aesthetic Surgery, University Hospital of Lausanne (CHUV), Lausanne, Switzerland.
authors.forename: Wassim
authors.initials: W
authors.lastname: Raffoul
authors.affiliation: Department of Plastic, Reconstructive and Aesthetic Surgery, University Hospital of Lausanne (CHUV), Lausanne, Switzerland; Department of ORL, Head and Neck Surgery, University Hospital of Bern, Bern, Switzerland. Electronic address: erbapaolo@hotmail.com.
authors.forename: Paolo
authors.initials: P
authors.lastname: Erba
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D053687:Adult Stem Cells / Q000637:transplantation; D000818:Animals; D016319:Corrosion Casting; D004195:Disease Models, Animal; D006801:Humans; D042583:Lymphangiogenesis; D042601:Lymphatic Vessels / Q000473:pathology / Q000503:physiopathology; D008209:Lymphedema / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D053657:Platelet-Rich Plasma; D012038:Regeneration; D013623:Tail
keywords: Adipose stem cells; Angiogenesis; Corrosion casting; Lymphatic regeneration; Lymphoedema; PRP