title: Interleukin-27 enhances TNF-α-mediated activation of human coronary artery endothelial cells.
abstract: Immune cells and inflammatory mediators play important roles in the development of atherosclerotic vascular inflammation. IL-27 is a member of the IL-6/IL-12 family that can promote Th1 responses and augment the release of inflammatory mediators from human mast cells and monocytes. However, the direct effect of IL-27 on human coronary artery endothelial cells was unclear. In this study, the effects of IL-27 and TNF-α on the cell surface expression of adhesion molecules, inflammatory cytokines, and chemokines were investigated. Results showed that IL-27 alone could significantly promote the release of chemokine CXCL10. However, IL-27 could further significantly enhance the TNF-α-mediated upregulation of adhesion molecules ICAM-1 and VCAM-1, inflammatory cytokine IL-6, as well as chemokines CCL5 and CXCL10 from human coronary artery endothelial cells. The release of IL-6, CCL5, and CXCL10 were significantly suppressed by specific signaling molecule inhibitors, implying that the induction of these mediators from the human coronary artery endothelial cells could be differentially regulated by the c-Jun N-terminal kinase, p38 mitogen-activated protein kinase, and nuclear factor-κB pathways. These results provided new insights into the effect of IL-27 on the TNF-α mediated activation of human coronary artery endothelial cells in atherosclerotic vascular inflammation.
authors.affiliation: Guangzhou Institute of Cardiovascular Disease, The Second Hospital Affiliated to Guangzhou Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Huai-Na
authors.initials: HN
authors.lastname: Qiu
authors.affiliation: Guangzhou Institute of Cardiovascular Disease, The Second Hospital Affiliated to Guangzhou Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Guangzhou Institute of Cardiovascular Disease, The Second Hospital Affiliated to Guangzhou Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Liu
authors.affiliation: Guangzhou Institute of Cardiovascular Disease, The Second Hospital Affiliated to Guangzhou Medical University, Guangzhou, Guangdong Province, China. gzliushiming@126.com.
authors.forename: Shiming
authors.initials: S
authors.lastname: Liu
mesh_terms: D057134:Antibodies, Neutralizing / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D064094:Interleukin-27 / Q000502:physiology*; D020935:MAP Kinase Signaling System; D016328:NF-kappa B / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
chemical_list: D057134:Antibodies, Neutralizing; D015815:Cell Adhesion Molecules; D016207:Cytokines; D064094:Interleukin-27; D016328:NF-kappa B; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha
keywords: Chemokine; Cytokine; HCAEC; Vascular inflammation