title: Epigallocatechin gallate affects glucose metabolism and increases fitness and lifespan in Drosophila melanogaster.
abstract: In this study, we tested whether a standardized epigallocatechin-3-gallate (EGCG) rich green tea extract (comprising > 90% EGCG) affects fitness and lifespan as well as parameters of glucose metabolism and energy homeostasis in the fruit fly, Drosophila melanogaster. Following the application of the green tea extract a significant increase in the mean lifespan (+ 3.3 days) and the 50% survival (+ 4.3 days) as well as improved fitness was detected. These effects went along an increased expression of Spargel, the homolog of mammalian PGC1α, which has been reported to affect lifespan in flies. Intriguingly, in flies, treatment with the green tea extract decreased glucose concentrations, which were accompanied by an inhibition of α-amylase and α-glucosidase activity. Computational docking analysis proved the potential of EGCG to dock into the substrate binding pocket of α-amylase and to a greater extent into α-glucosidase. Furthermore, we demonstrate that EGCG downregulates insulin-like peptide 5 and phosphoenolpyruvate carboxykinase, major regulators of glucose metabolism, as well as the Drosophila homolog of leptin, unpaired 2. We propose that a decrease in glucose metabolism in connection with an upregulated expression of Spargel contribute to the better fitness and the extended lifespan in EGCG-treated flies.
authors.affiliation: Institute of Human Nutrition and Food Science, University of Kiel, Kiel, Germany.
authors.forename: Anika E
authors.initials: AE
authors.lastname: Wagner
authors.affiliation: Institute of Human Nutrition and Food Science, University of Kiel, Kiel, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Piegholdt
authors.affiliation: Institute of Human Nutrition and Food Science, University of Kiel, Kiel, Germany.
authors.forename: Doerte
authors.initials: D
authors.lastname: Rabe
authors.affiliation: Institute of Human Nutrition and Food Science, University of Kiel, Kiel, Germany.|Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, Murcia, Spain.
authors.forename: Nieves
authors.initials: N
authors.lastname: Baenas
authors.affiliation: Institute of Human Nutrition and Food Science, University of Kiel, Kiel, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Schloesser
authors.affiliation: DSM Nutritional Products, Basel, Switzerland.
authors.forename: Manfred
authors.initials: M
authors.lastname: Eggersdorfer
authors.affiliation: Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland.
authors.forename: Achim
authors.initials: A
authors.lastname: Stocker
authors.affiliation: Institute of Human Nutrition and Food Science, University of Kiel, Kiel, Germany.
authors.forename: Gerald
authors.initials: G
authors.lastname: Rimbach
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D028241:Camellia sinensis / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D029721:Drosophila Proteins / Q000096:biosynthesis; D004331:Drosophila melanogaster / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism*; D006706:Homeostasis / Q000187:drug effects; D007328:Insulin / Q000096:biosynthesis; D008136:Longevity / Q000187:drug effects / Q000502:physiology*; D062105:Molecular Docking Simulation; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000096:biosynthesis; D010936:Plant Extracts / Q000494:pharmacology; D042925:Positive Transcriptional Elongation Factor B / Q000096:biosynthesis; D011506:Proteins; D014157:Transcription Factors / Q000096:biosynthesis; D015854:Up-Regulation; D000516:alpha-Amylases / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D029721:Drosophila Proteins; D007328:Insulin; C084797:Leydig insulin-like protein; D010936:Plant Extracts; D011506:Proteins; D014157:Transcription Factors; C527201:pgc protein, Drosophila; C115570:upd1 protein, Drosophila; D002392:Catechin; C045651:epigallocatechin gallate; D042925:Positive Transcriptional Elongation Factor B; D000516:alpha-Amylases; D000520:alpha-Glucosidases; D019764:Phosphoenolpyruvate Carboxykinase (ATP); D005947:Glucose
keywords: Drosophila melanogaster; Gerotarget; epigallocathechin-3-gallate; green tea; life span