title: [MTA1-specific shRNA inhibits the growth and metastasis of mouse xenograft of laryngeal squamous cell carcinoma].
abstract: OBJECTIVE
  To observe the effect of metastasis-associated gene 1 (MTA1) on the growth and metastasis of laryngeal squamous cell carcinoma in nude mice using RNA interference technology (RNAi).
  
  
  METHODS
  Lentiviral vector of short hairpin RNA (shRNA) targeting MTA1 was constructed and packaged to transfect Hep-2 cells. Hep-2 cells transfected with scramble shRNA and MTA1 shRNA were injected into the paw pad of nude mice (n=5 per group). Nine weeks after modeling of the lymphatic metastasis of laryngeal carcinoma, the mice were sacrificed, and tumor tissues and inguinal lymph node were harvested to be subjected to HE staining, reverse transcription PCR and Western blotting.
  
  
  RESULTS
  The gene screening showed the lentiviral vector of MTA1 shRNA was constructed successfully, and those tumor cells were transplanted and grew well in all mice. The size of tumor in the mice of MTA1 shRNA tansfected group was obviously smaller compared to the scramble shRNA transfected group at the same time point. No inguinal lymph node metastasis was found in the mice of MTA1 shRNA group. In contrast, the tumor cells were seen in the inguinal lymph nodes of the scramble shRNA infected mice. Reverse transcription PCR and Western blotting showed that the mRNA and protein levels of MTA1, β-catenin, matrix metalloproteinase-9 (MMP-9), cyclin D1 were obviously reduced in MTA1 shRNA infected mice compared with the scramble shRNA infected mice.
  
  
  CONCLUSIONS
  The inhibition of MTA1 gene could depress the growth and metastasis of laryngeal squamous cell carcinoma in nude mice.
authors.affiliation: Laboratory Center for Otorhinolaryngology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Laboratory Center for Otorhinolaryngology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Laboratory Center for Otorhinolaryngology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Laboratory Center for Otorhinolaryngology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Bai
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000032:analysis / Q000235:genetics; D005260:Female; D006258:Head and Neck Neoplasms / Q000473:pathology*; D007822:Laryngeal Neoplasms / Q000473:pathology*; D008207:Lymphatic Metastasis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D034741:RNA, Small Interfering / Q000235:genetics*; D012097:Repressor Proteins; D000077195:Squamous Cell Carcinoma of Head and Neck; D015534:Trans-Activators; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000502:physiology*; D051176:beta Catenin / Q000032:analysis / Q000235:genetics
chemical_list: C503336:Mta1 protein, mouse; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D051176:beta Catenin; D019938:Cyclin D1; D020780:Matrix Metalloproteinase 9