title: Genomic Diversity and Evolution of Bacillus subtilis.
abstract: Bacillus subtilis is the focus of both academic and industrial research. Previous studies have reported a number of sequence variations in different B. subtilis strains. To uncover the genetic variation and evolutionary pressure in B. subtilis strains, we performed whole genome sequencing of two B. subtilis isolates, KM and CGMCC63528. Comparative genomic analyses of these two strains with other B. subtilis strains identified high sequence variations including large insertions, deletions and SNPs. Most SNPs in genes were synonymous and the average frequency of synonymous mutations was significantly higher than that of the non-synonymous mutations. Pan-genome analysis of B. subtilis strains showed that the core genome had lower dN/dS values than the accessory genome. Whole genome comparisons of these two isolates with other B. subtilis strains showed that strains in different subspecies have similar dN/dS values. Nucleotide diversity analysis showed that spizizenii subspecies have higher nucleotide diversity than subtilis subspecies. Our results indicate that genes in B. subtilis strains are under high purifying selection pressure. The evolutionary pressure in different subspecies of B. subtilis is complex.
authors.affiliation: The State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China; Division of Enteric Bacterial Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Yu
authors.affiliation: Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua university, Beijing 100084, China.
authors.forename: Xun Cheng
authors.initials: XC
authors.lastname: Wang
authors.affiliation: Division of Enteric Bacterial Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China.
authors.forename: Wang Hong
authors.initials: WH
authors.lastname: Tian
authors.affiliation: Division of Enteric Bacterial Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China.
authors.forename: Ji Chun
authors.initials: JC
authors.lastname: Shi
authors.affiliation: Division of Enteric Bacterial Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Division of Enteric Bacterial Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ye
authors.affiliation: Division of Enteric Bacterial Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China.
authors.forename: Si Guo
authors.initials: SG
authors.lastname: Dong
authors.affiliation: Division of Enteric Bacterial Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zeng
authors.affiliation: The State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China; Division of Enteric Bacterial Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China.
authors.forename: Jun Zhi
authors.initials: JZ
authors.lastname: Wang
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D019143:Evolution, Molecular; D005798:Genes, Bacterial; D020641:Polymorphism, Single Nucleotide