title: Transcriptome profiling of postharvest strawberry fruit in response to exogenous auxin and abscisic acid.
abstract: Auxin and abscisic acid regulate strawberry fruit ripening and senescence through cross-talk of their signal transduction pathways that further modulate the structural genes related to physico-chemical properties of fruit. The physiological and transcriptomic changes in harvested strawberry fruits in responses to IAA, ABA and their combination were analyzed. Exogenous IAA delayed the ripening process of strawberries after harvest while ABA promoted the postharvest ripening. However, treatment with a combination of IAA and ABA did not slow down nor accelerate the postharvest ripening in the strawberry fruits. At the molecular level, exogenous IAA up regulated the expressions of genes related to IAA signaling, including AUX/IAA, ARF, TOPLESS and genes encoding E3 ubiquitin protein ligase and annexin, and down regulated genes related to pectin depolymerization, cell wall degradation, sucrose and anthocyanin biosyntheses. In contrast, exogenous ABA induced genes related to fruit softening, and genes involved in signaling pathways including SKP1, HSPs, CK2, and SRG1. Comparison of transcriptomes in responses to individual treatments with IAA or ABA or the combination revealed that there were cooperative and antagonistic actions between IAA and ABA in fruit. However, 17% of the differentially expressed unigenes in response to the combination of IAA and ABA were unique and were not found in those unigenes responding to either IAA or ABA alone. The analyses also found that receptor-like kinases and ubiquitin ligases responded to both IAA and ABA, which seemed to play a pivotal role in both hormones' signaling pathways and thus might be the cross-talk points of both hormones.
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory of Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, China. 11213034@zju.edu.cn.
authors.forename: Jingxin
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory of Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, China. linchun@zju.edu.cn.
authors.forename: Linchun
authors.initials: L
authors.lastname: Mao
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory of Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Lu
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory of Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Tiejin
authors.initials: T
authors.lastname: Ying
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory of Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Zisheng
authors.initials: Z
authors.lastname: Luo
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology; D015536:Down-Regulation; D031985:Fragaria / Q000187:drug effects / Q000235:genetics*; D005638:Fruit / Q000187:drug effects / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics*; D007210:Indoleacetic Acids / Q000378:metabolism / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013395:Sucrose / Q000378:metabolism; D059467:Transcriptome
chemical_list: D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D013395:Sucrose; D000040:Abscisic Acid
keywords: Abscisic acid; Auxin; Fragaria × ananassa; Postharvest senescence; Signal transduction; Transcriptome