title: Heat-Induced Fragmentation and Adapter-Assisted Whole Genome Amplification Using GenomePlex® Single-Cell Whole Genome Amplification Kit (WGA4).
abstract: Whole genome amplification (WGA) is a widely used technique allowing multiplying picogram amounts of target DNA by several orders of magnitude. The technique described here is based on heat-induced random fragmentation yielding DNA strands mainly ranging from 0.1 to 1 kb in length. The fragmented DNA is then subjected to library generation by annealing of adaptor sequences to both ends of the DNA fragments. Using primers hybridizing to the adapter sequences, the DNA is amplified by thermal cycling. This amplification typically yields > 2 mg DNA from a single cell, is suited for amplifying DNA isolated from (partly) degraded samples [e.g. formalin-fixed paraffin-embedded (FFPE) material] and works well when used for array-comparative genome hybridization (array-CGH).
authors.affiliation: Research Unit for Single Cell Analysis, Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Harrachgasse 21, Graz, 8010, Austria.
authors.forename: Amin
authors.initials: A
authors.lastname: El-Heliebi
authors.affiliation: Research Unit for Single Cell Analysis, Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Harrachgasse 21, Graz, 8010, Austria.
authors.forename: Shukun
authors.initials: S
authors.lastname: Chen
authors.affiliation: Research Unit for Single Cell Analysis, Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Harrachgasse 21, Graz, 8010, Austria. thomas.kroneis@medunigraz.at.|Sahlgrenska Cancer Center, University of Gothenburg, Gothenburg, Sweden. thomas.kroneis@medunigraz.at.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kroneis
mesh_terms: D053938:DNA Fragmentation; D016678:Genome; D023281:Genomics / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques; D011933:Reagent Kits, Diagnostic; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D011933:Reagent Kits, Diagnostic
keywords: FFPE samples; GenomePlex; Single-cell analysis; Single-cell whole genome amplification; Whole genome amplification