title: Assessment of Oxidative Stress in Septic and Obese Patients Using Markers of Oxidation-reduction Potential.
abstract: OBJECTIVE
  The novel static (sORP) and capacity (cORP) oxidation-reduction potential markers were examined for assessing oxidative stress in plasma of patients with sepsis. Moreover, the possible effect of obesity-induced oxidative stress on patients with sepsis was investigated.
  
  
  METHODS
  sORP and cORP markers, as well as the conventional oxidative stress biomarkers total antioxidant capacity (TAC), thiobarbituric acid-reactive substances (TBARS) and protein carbonyls (CARB), were assessed in plasma.
  
  
  RESULTS
  sORP marker was increased significantly in the sepsis group, while cORP was significantly lower compared to the control group, indicating oxidative stress. Furthermore, in patients with sepsis, TAC was significantly lower compared to control group. However, obesity had no effect on sORP, cORP and TAC in patients with sepsis, although it increased levels of CARB and TBARS.
  
  
  CONCLUSIONS
  The present results suggest, for the first time, that ORP markers could be used for assessing oxidative stress in patients with sepsis.
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
authors.forename: Ypatios
authors.initials: Y
authors.lastname: Spanidis
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Goutzourelas
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Stagos
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, Patras University Hospital, Rion-Patras, Greece.
authors.forename: Anastasia S
authors.initials: AS
authors.lastname: Kolyva
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, Patras University Hospital, Rion-Patras, Greece.
authors.forename: Charalambos A
authors.initials: CA
authors.lastname: Gogos
authors.affiliation: Trauma Research Department, St. Anthony Hospital, Lakewood, CO, U.S.A. Trauma Research Department, Swedish Medical Center, Englewood, CO, U.S.A. Trauma Research Department, Medical Center of Plano, Plano, TX, U.S.A. Luoxis Diagnostics, Inc., Englewood, CO, U.S.A.
authors.forename: David
authors.initials: D
authors.lastname: Bar-Or
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece dkouret@uth.gr.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Kouretas
mesh_terms: D015415:Biomarkers; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D009765:Obesity / Q000097:blood / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D018805:Sepsis / Q000097:blood / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D015415:Biomarkers; D017392:Thiobarbituric Acid Reactive Substances
keywords: Oxidative stress; biomarkers; obesity; oxidation-reduction potential; sepsis