title: Towards monitoring the sandflies (Diptera: Psychodidae) of Thailand: DNA barcoding the sandflies of Wihan Cave, Uttaradit.
abstract: Sandflies vary in their distributions and role in pathogen transmission. Attempts to record distributions of sandflies in Thailand have faced difficulties due to their high abundance and diversity. We aim to provide an insight into the diversity of sandflies in Thailand by (i) conducting a literature review, and (ii) DNA barcoding sandflies collected from Wihan Cave where eight morphologically characterized species were recorded. DNA barcodes generated for 193 sandflies fell into 13 distinct species clusters under four genera (Chinius, Idiophlebotomus, Phlebotomus and Sergentomyia). Five of these species could be assigned Linnaean species names unambiguously and two others corresponded to characterized morphospecies. Two species represented a complex under the name Sergentomyia barraudi while the remaining four had not been recognized before in any form. The resulting species checklist and DNA barcode library contribute to a growing set of records for sandflies which is useful for monitoring and vector control.
authors.affiliation: a Department of Microbiology and Parasitology, Faculty of Medical Science , Naresuan University , Phitsanulok , Thailand .|b Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University , Phitsanulok , Thailand .
authors.forename: Raxsina
authors.initials: R
authors.lastname: Polseela
authors.affiliation: a Department of Microbiology and Parasitology, Faculty of Medical Science , Naresuan University , Phitsanulok , Thailand .|c Department of Parasitology, Faculty of Medicine , University of Malaya , Kuala Lumpur , Malaysia .
authors.forename: Narong
authors.initials: N
authors.lastname: Jaturas
authors.affiliation: a Department of Microbiology and Parasitology, Faculty of Medical Science , Naresuan University , Phitsanulok , Thailand .|b Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University , Phitsanulok , Thailand .
authors.forename: Aunchalee
authors.initials: A
authors.lastname: Thanwisai
authors.affiliation: d Ecology and Biodiversity Program, Institute of Biological Sciences, Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia , and.|e Museum of Zoology, Institute of Biological Sciences, Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: Kong-Wah
authors.initials: KW
authors.lastname: Sing
authors.affiliation: d Ecology and Biodiversity Program, Institute of Biological Sciences, Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia , and.|e Museum of Zoology, Institute of Biological Sciences, Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: John-James
authors.initials: JJ
authors.lastname: Wilson
mesh_terms: D063147:Animal Distribution; D000818:Animals; D001483:Base Sequence; D059552:Caves; D058893:DNA Barcoding, Taxonomic; D017344:Genes, Insect; D014644:Genetic Variation; D019476:Insect Proteins / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011576:Psychodidae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013785:Thailand
chemical_list: D019476:Insect Proteins
keywords: DNA barcoding; Thailand; leishmaniasis; sandflies