title: A Novel Detection Platform for Shrimp White Spot Syndrome Virus Using an ICP11-Dependent Immunomagnetic Reduction (IMR) Assay.
abstract: Shrimp white spot disease (WSD), which is caused by white spot syndrome virus (WSSV), is one of the world's most serious shrimp diseases. Our objective in this study was to use an immunomagnetic reduction (IMR) assay to develop a highly sensitive, automatic WSSV detection platform targeted against ICP11 (the most highly expressed WSSV protein). After characterizing the magnetic reagents (Fe3O4 magnetic nanoparticles coated with anti ICP11), the detection limit for ICP11 protein using IMR was approximately 2 x 10(-3) ng/ml, and the linear dynamic range of the assay was 0.1~1 x 10(6) ng/ml. In assays of ICP11 protein in pleopod protein lysates from healthy and WSSV-infected shrimp, IMR signals were successfully detected from shrimp with low WSSV genome copy numbers. We concluded that this IMR assay targeting ICP11 has potential for detecting the WSSV.
authors.affiliation: MagQu Co., Ltd., New Taipei City 231, Taiwan.
authors.forename: Bing-Hsien
authors.initials: BH
authors.lastname: Liu
authors.affiliation: MagQu Co., Ltd., New Taipei City 231, Taiwan.
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Lin
authors.affiliation: MagQu Co., Ltd., New Taipei City 231, Taiwan.
authors.forename: Chia-Shin
authors.initials: CS
authors.lastname: Ho
authors.affiliation: MagQu Co., Ltd., New Taipei City 231, Taiwan.
authors.forename: Che-Chuan
authors.initials: CC
authors.lastname: Yang
authors.affiliation: MagQu Co., Ltd., New Taipei City 231, Taiwan.
authors.forename: Yun-Tsui
authors.initials: YT
authors.lastname: Chang
authors.affiliation: MagQu Co., Ltd., New Taipei City 231, Taiwan.
authors.forename: Jui-Feng
authors.initials: JF
authors.lastname: Chang
authors.affiliation: Institute of Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Chun-Yuan
authors.initials: CY
authors.lastname: Li
authors.affiliation: Institute of Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Cheng-Shun
authors.initials: CS
authors.lastname: Cheng
authors.affiliation: Institute of Bioinformatics and Biosignal Transduction, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Jiun-Yan
authors.initials: JY
authors.lastname: Huang
authors.affiliation: MagQu Co., Ltd., New Taipei City 231, Taiwan.
authors.forename: Yen-Fu
authors.initials: YF
authors.lastname: Lee
authors.affiliation: MagQu Co., Ltd., New Taipei City 231, Taiwan.
authors.forename: Ming-Hung
authors.initials: MH
authors.lastname: Hsu
authors.affiliation: MagQu Co., Ltd., New Taipei City 231, Taiwan.
authors.forename: Feng-Chun
authors.initials: FC
authors.lastname: Lin
authors.affiliation: Graduate Institute of Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Hao-Ching
authors.initials: HC
authors.lastname: Wang
authors.affiliation: Institute of Bioinformatics and Biosignal Transduction, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Chu-Fang
authors.initials: CF
authors.lastname: Lo
authors.affiliation: MagQu Co., Ltd., New Taipei City 231, Taiwan.
authors.forename: Shieh-Yueh
authors.initials: SY
authors.lastname: Yang
authors.affiliation: Institute of Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Han-Ching
authors.initials: HC
authors.lastname: Wang
mesh_terms: D000820:Animal Diseases / Q000175:diagnosis / Q000821:virology; D000818:Animals; D060829:Arthropod Proteins / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D047468:Immunoprecipitation / Q000379:methods* / Q000662:veterinary; D057230:Limit of Detection; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry; D033561:Penaeidae / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D060829:Arthropod Proteins; D058185:Magnetite Nanoparticles; C548473:VP28 protein, white spot syndrome virus; D014759:Viral Envelope Proteins