title: High-quality permanent draft genome sequence of Ensifer meliloti strain 4H41, an effective salt- and drought-tolerant microsymbiont of Phaseolus vulgaris.
abstract: Ensifer meliloti 4H41 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of common bean (Phaseolus vulgaris). Strain 4H41 was isolated in 2002 from root nodules of P. vulgaris grown in South Tunisia from the oasis of Rjim-Maatoug. Strain 4H41 is salt- and drought-tolerant and highly effective at fixing nitrogen with P. vulgaris. Here we describe the features of E. meliloti 4H41, together with genome sequence information and its annotation. The 6,795,637 bp high-quality permanent draft genome is arranged into 47 scaffolds of 47 contigs containing 6,350 protein-coding genes and 72 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.
authors.affiliation: Laboratory of Legumes, Centre of Biotechnology of Borj-Cedria, BP 901, Hammam-Lif, 2050 Tunisia.
authors.forename: Ridha
authors.initials: R
authors.lastname: Mhamdi
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Murdoch, WA Australia.
authors.forename: Julie
authors.initials: J
authors.lastname: Ardley
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Murdoch, WA Australia.
authors.forename: Rui
authors.initials: R
authors.lastname: Tian
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Rekha
authors.initials: R
authors.lastname: Seshadri
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: T B K
authors.initials: TB
authors.lastname: Reddy
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Amrita
authors.initials: A
authors.lastname: Pati
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, CA USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Markowitz
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA ; Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Nikos
authors.initials: N
authors.lastname: Kyrpides
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Murdoch, WA Australia.
authors.forename: Wayne
authors.initials: W
authors.lastname: Reeve
keywords: Alphaproteobacteria; Ensifer; Nitrogen fixation; Phaseolus vulgaris; Root-nodule bacteria