title: Autoantibody Profiling of Glioma Serum Samples to Identify Biomarkers Using Human Proteome Arrays.
abstract: The heterogeneity and poor prognosis associated with gliomas, makes biomarker identification imperative. Here, we report autoantibody signatures across various grades of glioma serum samples and sub-categories of glioblastoma multiforme using Human Proteome chips containing ~17000 full-length human proteins. The deduced sets of classifier proteins helped to distinguish Grade II, III and IV samples from the healthy subjects with 88, 89 and 94% sensitivity and 87, 100 and 73% specificity, respectively. Proteins namely, SNX1, EYA1, PQBP1 and IGHG1 showed dysregulation across various grades. Sub-classes of GBM, based on its proximity to the sub-ventricular zone, have been reported to have different prognostic outcomes. To this end, we identified dysregulation of NEDD9, a protein involved in cell migration, with probable prognostic potential. Another subcategory of patients where the IDH1 gene is mutated, are known to have better prognosis as compared to patients carrying the wild type gene. On a comparison of these two cohorts, we found STUB1 and YWHAH proteins dysregulated in Grade II glioma patients. In addition to common pathways associated with tumourigenesis, we found enrichment of immunoregulatory and cytoskeletal remodelling pathways, emphasizing the need to explore biochemical alterations arising due to autoimmune responses in glioma.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Parvez
authors.initials: P
authors.lastname: Syed
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Shabarni
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Saket
authors.initials: S
authors.lastname: Choudhary
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Narendra Goud
authors.initials: NG
authors.lastname: Pandala
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Apurva
authors.initials: A
authors.lastname: Atak
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Annie
authors.initials: A
authors.lastname: Richharia
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Manubhai
authors.initials: M
authors.lastname: K P
authors.affiliation: Department of Pharmacology and Molecular Sciences/High-Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Pathology, Tata Memorial Centre, Mumbai 400 012, India.
authors.forename: Sridhar
authors.initials: S
authors.lastname: Epari
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Santosh B
authors.initials: SB
authors.lastname: Noronha
authors.affiliation: Department of Neurosurgery, Tata Memorial Centre, Mumbai 400 012, India.
authors.forename: Aliasgar
authors.initials: A
authors.lastname: Moiyadi
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Sanjeeva
authors.initials: S
authors.lastname: Srivastava
mesh_terms: D001323:Autoantibodies / Q000097:blood* / Q000276:immunology*; D015415:Biomarkers; D005910:Glioma / Q000097:blood* / Q000276:immunology* / Q000473:pathology; D006801:Humans; D060787:Neoplasm Grading; D020543:Proteome; D040901:Proteomics / Q000379:methods
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D020543:Proteome