title: Antibacterial Activities of Ankaferd Hemostat (ABS) on Shiga Toxin-Producing Escherichia coli and Other Pathogens Significant in Foodborne Diseases.
abstract: OBJECTIVE
  Ankaferd hemostat (Ankaferd Blood Stopper®, ABS)-induced pharmacological modulation of essential erythroid proteins can cause vital erythroid aggregation via acting on fibrinogen gamma. Topical endoscopic ABS application is effective in the controlling of gastrointestinal (GI) system hemorrhages and/or infected GI wounds. Escherichia coli O157:H7, the predominant serotype of enterohemorrhagic E. coli, is a cause of both outbreaks and sporadic cases of hemorrhagic colitis. The aim of this study is to examine the effects of ABS on 6 different Shiga toxigenic E. coli serotypes including O26, O103, O104, O111, O145, and O157 and on other pathogens significant in foodborne diseases, such as Salmonella Typhimurium, Campylobacter jejuni, and Listeria monocytogenes, were also assessed.
  
  
  METHODS
  All strains were applied with different amounts of ABS and antimicrobial effect was screened. S. Typhimurium groups were screened for survival using the fluorescence in situ hybridization technique.
  
  
  RESULTS
  The relative efficacy of ABS solutions to achieve significant logarithmic reduction in foodborne pathogens E. coli O157:H7 and non-O157 serogroups and other emerging foodborne pathogens is demonstrated in this study. ABS has antibacterial effects.
  
  
  CONCLUSIONS
  Our present study indicated for the first time that ABS may act against E. coli O157:H7, which is a cause of thrombotic thrombocytopenic purpura, hemolytic-uremic syndrome, and hemorrhagic colitis. The interrelationships between colitis, infection, and hemostasis within the context of ABS application should be further investigated in future studies.
authors.affiliation: Republic of Turkey Ministry of Food, National Food Reference Laboratory, Department of Mineral Analyses, Agriculture, and Livestock, Ankara, Turkey E-mail : ahmetkoluman@hotmail.com.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Koluman
authors.forename: Nejat
authors.initials: N
authors.lastname: Akar
authors.forename: İbrahim C
authors.initials: İC
authors.lastname: Haznedaroğlu
mesh_terms: D016123:Campylobacter jejuni / Q000302:isolation & purification; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008089:Listeria monocytogenes / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry*; D012486:Salmonella typhimurium / Q000302:isolation & purification; D012703:Serotyping; D054323:Shiga-Toxigenic Escherichia coli / Q000302:isolation & purification*
chemical_list: D010936:Plant Extracts; C539751:ankaferd blood stopper