title: Lentzea guizhouensis sp. nov., a novel lithophilous actinobacterium isolated from limestone from the Karst area, Guizhou, China.
abstract: A novel filamentous actinobacterium, designated strain DHS C013(T), was isolated from limestone collected in Guizhou Province, South-west China. Morphological and chemotaxonomic characteristics of the strain support its assignment to the genus Lentzea. Phylogenetic analyses showed that strain DHS C013(T) is closely related to Lentzea jiangxiensis FXJ1.034(T) (98.7 % 16S rRNA gene similarity) and Lentzea flaviverrucosa 4.0578(T) (98.0 % 16S rRNA gene similarity), but it can be distinguished from these strains based on low levels of DNA:DNA relatedness (~44 and ~37 %, respectively). Physiological and biochemical tests also allowed phenotypic differentiation of the novel strain from these closely related species. On the basis of the evidence presented here, strain DHS C013(T) is concluded to represent a novel species of the genus Lentzea, for which the name Lentzea guizhouensis sp. nov. is proposed. The type strain is DHS C013(T) (=KCTC 29677(T) = CGMCC 4.7203(T)).
authors.affiliation: State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, Guizhou, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, People's Republic of China.|The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, People's Republic of China.
authors.forename: Cheng-Liang
authors.initials: CL
authors.lastname: Cao
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, People's Republic of China.
authors.forename: Xiao-Qi
authors.initials: XQ
authors.lastname: Zhou
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, People's Republic of China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, Guizhou, People's Republic of China.
authors.forename: Fa-Xiang
authors.initials: FX
authors.lastname: Tao
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, People's Republic of China.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, Guizhou, People's Republic of China. bin2368@vip.163.com.|College of Life Science, Nanjing Normal University, Nanjing, Jiangsu, People's Republic of China. bin2368@vip.163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Lian
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D002119:Calcium Carbonate; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D002119:Calcium Carbonate
keywords: Karst area; Lentzea guizhouensis sp. nov.; Lithophilous; Polyphasic taxonomy