title: Regulation of the chitin degradation and utilization system by the ChiX small RNA in Serratia marcescens 2170.
abstract: Serratia marcescens 2170 produces three different types of chitinases and chitin-binding protein CBP21. We found that transposon insertion into the 5' untranslated region (5' UTR) of chiPQ-ctb led to defective chitinase and CBP21 production. ChiX small RNA possessed the complementary sequence of the 5' UTRs of the chiPQ-ctb and chiR and repressed the expression of chiP and chiR. ChiX was detected in a medium containing glucose, glycerol, GlcNAc, and (GlcNAc)2, but the expression of both chiP and chiR was only observed in a medium containing (GlcNAc)2. ∆chiX mutant produced chitinases, CBP21, and chitobiase without induction. chiP transcripts were more abundant than those of chiR or chiX in a medium containing (GlcNAc)2. These results suggest that the constitutively expressed ChiX binds to the highly abundant chiP 5' UTR, thereby leading to the induction of chiR mRNA translation and the subsequent expression of chitinases and CBP21.
authors.affiliation: a Graduate School of Science and Technology , Niigata University , Niigata , Japan.|b Faculty of Agriculture, Department of Applied Biological Chemistry , Niigata University , Niigata , Japan.
authors.forename: Kazushi
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: a Graduate School of Science and Technology , Niigata University , Niigata , Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Shimizu
authors.affiliation: a Graduate School of Science and Technology , Niigata University , Niigata , Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Sasaki
authors.affiliation: a Graduate School of Science and Technology , Niigata University , Niigata , Japan.
authors.forename: Chisana
authors.initials: C
authors.lastname: Ogawa
authors.affiliation: a Graduate School of Science and Technology , Niigata University , Niigata , Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Minami
authors.affiliation: a Graduate School of Science and Technology , Niigata University , Niigata , Japan.|b Faculty of Agriculture, Department of Applied Biological Chemistry , Niigata University , Niigata , Japan.
authors.forename: Hayuki
authors.initials: H
authors.lastname: Sugimoto
authors.affiliation: a Graduate School of Science and Technology , Niigata University , Niigata , Japan.|b Faculty of Agriculture, Department of Applied Biological Chemistry , Niigata University , Niigata , Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D020121:5' Untranslated Regions; D000117:Acetylglucosamine / Q000378:metabolism; D000118:Acetylglucosaminidase / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006868:Hydrolysis; D047908:Intracellular Signaling Peptides and Proteins; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D058727:RNA, Small Untranslated / Q000235:genetics* / Q000378:metabolism; D012706:Serratia marcescens / Q000235:genetics* / Q000378:metabolism
chemical_list: D020121:5' Untranslated Regions; D001426:Bacterial Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D007527:Isoenzymes; D058727:RNA, Small Untranslated; C111062:chitin-binding protein 21, Serratia marcescens; D002686:Chitin; D002688:Chitinases; D000118:Acetylglucosaminidase; D005947:Glucose; D005990:Glycerol; D000117:Acetylglucosamine
keywords: 5′ untranslated region (5′ UTR); Serratia marcescens; chitinase; chitobiase; small RNA