title: Spatiotemporal regulation of coagulation and platelet activation during the hemostatic response in vivo.
abstract: The hemostatic response requires the tightly regulated interaction of the coagulation system, platelets, other blood cells and components of the vessel wall at a site of vascular injury. The dysregulation of this response may result in excessive bleeding if the response is impaired, and pathologic thrombosis with vessel occlusion and tissue ischemia if the response is overly robust. Extensive studies over the past decade have sought to unravel the regulatory mechanisms that coordinate the multiple biochemical and cellular responses in time and space to ensure that an optimal response to vascular damage is achieved. These studies have relied in part on advances in in vivo imaging techniques in animal models, allowing for the direct visualization of various molecular and cellular events in real time during the hemostatic response. This review summarizes knowledge gained with these in vivo imaging and other approaches that provides new insights into the spatiotemporal regulation of coagulation and platelet activation at a site of vascular injury.
authors.affiliation: Department of Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
authors.forename: L
authors.initials: L
authors.lastname: Ivanciu
authors.affiliation: Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: T J
authors.initials: TJ
authors.lastname: Stalker
mesh_terms: D000244:Adenosine Diphosphate / Q000502:physiology; D000818:Animals; D030801:Animals, Genetically Modified; D001777:Blood Coagulation / Q000502:physiology*; D001779:Blood Coagulation Factors / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology; D055252:Cell-Derived Microparticles / Q000502:physiology; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D003094:Collagen / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology; D005456:Fluorescent Dyes; D017930:Genes, Reporter; D006801:Humans; D008024:Ligands; D051379:Mice; D018715:Microscopy, Video; D046912:Multiprotein Complexes; D019007:P-Selectin / Q000502:physiology; D015539:Platelet Activation / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled / Q000502:physiology; D013917:Thrombin / Q000096:biosynthesis; D013997:Time Factors; D057772:Vascular System Injuries / Q000097:blood
chemical_list: D001779:Blood Coagulation Factors; D005456:Fluorescent Dyes; D008024:Ligands; D046912:Multiprotein Complexes; D019007:P-Selectin; D043562:Receptors, G-Protein-Coupled; D000244:Adenosine Diphosphate; D003094:Collagen; D013917:Thrombin
keywords: animal models; blood coagulation; hemostasis; platelet activation; platelets; thrombosis