title: Nonviral Plasmid DNA Carriers Based on N,N'-Dimethylaminoethyl Methacrylate and Di(ethylene glycol) Methyl Ether Methacrylate Star Copolymers.
abstract: Star polymers with random and block copolymer arms made of cationic N,N'-dimethylaminoethyl methacrylate (DMAEMA) and nonionic di(ethylene glycol) methyl ether methacrylate (DEGMA) were synthesized via atom transfer radical polymerization (ATRP) and used for the delivery of plasmid DNA in gene therapy. All stars were able to form polyplexes with plasmid DNA. The structure and size of the polyplexes were precisely determined using light scattering and cryo-TEM microscopy. The hydrodynamic radius of a complex of DNA with star was dependent on the architecture of the star arms, the DEGMA content and the number of amino groups in the star compared to the number of phosphate groups of the nucleic acid (N/P ratio). The smallest polyplexes (Rh90°∼50 nm) with positive zeta potentials (∼15 mV) were formed of stars with N/P=6. The introduction of DEGMA into the star structure caused a decrease of polyplex cytotoxicity in comparison to DMAEMA homopolymer stars. The overall transfection efficiency using HT-1080 cells showed that the studied systems are prospective gene delivery agents. The most promising results were obtained for stars with random copolymer arms of high DEGMA content.
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , M. Curie-Sklodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Mendrek
authors.affiliation: Department of General, Molecular Biology and Genetics, Medical University of Silesia , Medykow 18, 40-752 Katowice, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Sieroń
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , M. Curie-Sklodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Żymełka-Miara
authors.affiliation: University of Occupational Safety Management in Katowice , ul. Bankowa 8, 40-007 Katowice, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Binkiewicz
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , M. Curie-Sklodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Libera
authors.affiliation: Department of Chemistry, University of Leuven , Celestijnenlaan, 200F, B-3001 Leuven (Heverlee), Belgium.
authors.forename: Mario
authors.initials: M
authors.lastname: Smet
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , M. Curie-Sklodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Trzebicka
authors.affiliation: Department of General, Molecular Biology and Genetics, Medical University of Silesia , Medykow 18, 40-752 Katowice, Poland.
authors.forename: Aleksander L
authors.initials: AL
authors.lastname: Sieroń
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , M. Curie-Sklodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kowalczuk
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , M. Curie-Sklodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Dworak
mesh_terms: D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage*; D005026:Ethylene Glycols / Q000737:chemistry*; D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D010957:Plasmids; D011108:Polymers / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D005026:Ethylene Glycols; D008689:Methacrylates; D011108:Polymers; C013484:diethylene glycol; D004247:DNA