title: Simultaneous Analysis of Tertiary Butylhydroquinone and 2-tert-Butyl-1,4-benzoquinone in Edible Oils by Normal-Phase High-Performance Liquid Chromatography.
abstract: During the process of antioxidation of tertiary butylhydroquinone (TBHQ) in oil and fat systems, 2-tert-butyl-1,4-benzoquinone (TQ) can be formed. The toxicity of TQ was much more than that of TBHQ. In the work, a normal-phase high-performance liquid chromatography (NP-HPLC) method for the accurate and simultaneous detection of TBHQ and TQ in edible oils was investigated. A C18 column was used to separate TBHQ and TQ, and the gradient elution solutions consisted of n-hexane containing 5% ethyl acetate and n-hexane containing 5% isopropanol. The ultraviolet (UV) detector was set at dual wavelength mode (280 nm for TBHQ and 310 nm for TQ). The column temperature was 30 °C. Before the NP-HPLC analysis, TBHQ and TQ were first extracted by methanol, subjected to vortex treatment, and then filtered through a 0.45 μm membrane filter. Results showed that linear ranges of TBHQ and TQ were both within 0.10-500.00 μg/mL (R(2) > 0.9999). The limit of detection (LOD) and limit of quantification (LOQ) of TBHQ and TQ were below 0.30 and 0.91 μg/mL and below 0.10 and 0.30 μg/mL, respectively. The recoveries of TBHQ and TQ were 98.92-102.34 and 96.28-100.58% for soybean oil and 96.11-99.42 and 98.83-99.24% for lard, respectively. These results showed that NP-HPLC can be successfully used to analyze simultaneously TBHQ and TQ in the oils and fats.
authors.affiliation: Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology , Lianhua Road, Zhengzhou, Henan 450001, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology , Lianhua Road, Zhengzhou, Henan 450001, People's Republic of China.
authors.forename: Yanlan
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology , Lianhua Road, Zhengzhou, Henan 450001, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology , Lianhua Road, Zhengzhou, Henan 450001, People's Republic of China.
authors.forename: Shangde
authors.initials: S
authors.lastname: Sun
mesh_terms: D016227:Benzoquinones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005503:Food Additives / Q000032:analysis*; D006873:Hydroquinones / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*
chemical_list: D016227:Benzoquinones; D005503:Food Additives; D006873:Hydroquinones; D010938:Plant Oils; C018855:2-tert-butylhydroquinone; C051290:2-tert-butyl-4-quinone
keywords: NP-HPLC; TBHQ; TQ; oxidation; reduction