title: A simple and high-resolution HPLC-PDA method for simultaneous quantification of local anesthetics in in vitro buccal permeation enhancement studies.
abstract: A simple, isocratic, high-resolution and prompt HPLC-PDA method was developed and validated for the simultaneous quantification of prilocaine (PCL) and lidocaine (LCL) hydrochlorides in in vitro buccal iontophoresis-driven permeation studies. A reversed-phase C18 column (250 mm x 4.6 mm, 3μm, 110Å) was used for the chromatographic separation. The mobile phase contained acetonitrile: 0.1M sodium phosphate buffer, pH 7.0 (1:1, v/v), plus 0.05% (v/v) diethylamine. The isocratic flow rate was set at 1 mL/min and the detection wavelength was 203 nm. PCL and LCL eluted in 8.9 min and 13 min, respectively, and the system suitability parameters varied within an acceptable range. The method was selective, sensitive, precise, accurate and robust, producing a linear plot at the concentration range of 0.25 to 10 µg/mL. The application of this method was demonstrated by a significant enhancement of the permeation of PCL and LCL with the application of iontophoresis (1 mA/cm(2) per 1 h) through isolated porcine esophageal epithelium. The amount of the drug retained in the epithelium also increased with the application of an electrical current. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903, Ribeirão Preto, SP, Brazil.
authors.forename: Renê O
authors.identifier: http://orcid.org/0000-0002-3748-3427
authors.initials: RO
authors.lastname: Couto
authors.affiliation: Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903, Ribeirão Preto, SP, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Cubayachi
authors.affiliation: Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903, Ribeirão Preto, SP, Brazil.
authors.forename: Renata F V
authors.initials: RF
authors.lastname: Lopez
authors.affiliation: Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903, Ribeirão Preto, SP, Brazil.
authors.forename: Cristiane M
authors.initials: CM
authors.lastname: de Gaitani
authors.affiliation: School of Odontology of Ribeirão Preto, University of São Paulo, 14040-904, Ribeirão Preto, SP, Brazil.
authors.forename: Vinícius
authors.initials: V
authors.lastname: Pedrazzi
authors.affiliation: Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903, Ribeirão Preto, SP, Brazil.
authors.forename: Osvaldo
authors.initials: O
authors.lastname: de Freitas
mesh_terms: D000779:Anesthetics, Local / Q000032:analysis* / Q000493:pharmacokinetics; D000818:Animals; D002610:Cheek; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004848:Epithelium / Q000378:metabolism; D004947:Esophagus / Q000378:metabolism; D066298:In Vitro Techniques; D057230:Limit of Detection; D015203:Reproducibility of Results; D013552:Swine
chemical_list: D000779:Anesthetics, Local
keywords: buccal permeation enhancement; iontophoresis; lidocaine hydrochloride; method validation; prilocaine hydrochloride