title: Fish Oil and Adjuvant-Induced Arthritis: Inhibitory Effect on Leukocyte Recruitment.
abstract: Fish oil, a rich source of n-3 fatty acids, has been studied for its beneficial effects in many diseases. Recent studies have shown the robust anti-inflammatory activity of fish oil (FO), when administered orally to rats, in models of acute inflammation. Herein, we investigated if treatment with fish oil preparation (FOP) could interfere with the recruitment of leukocytes into the joint cavity of arthritic rats. We also evaluated the effect of treatment on rolling behavior and leukocyte adhesion in vivo and on leukocyte chemotaxis in vitro. Treatment with FOP (75, 150, and 300 mg/kg) initiated on the day of induction of arthritis (day 0) and maintained for 21 days reduced the total number of leukocytes recruited into the joint cavity, the number of rolling and adhered leukocytes in arthritic rats, and leukocyte migration in response to stimulation with N-formyl-methionyl-leucyl-phenylalanine (fMLP) and leukotriene B4 (LTB4). Together, our data provide evidence that FOP plays an important inhibitory role in the recruitment of leukocytes into the joint cavity of arthritic rats.
authors.affiliation: Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil.
authors.forename: Camila Fernanda
authors.initials: CF
authors.lastname: Estevão-Silva
authors.affiliation: Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil.
authors.forename: Franciele Queiroz
authors.initials: FQ
authors.lastname: Ames
authors.affiliation: Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil.
authors.forename: Francielli Maria de Souza
authors.initials: FMS
authors.lastname: Silva-Comar
authors.affiliation: Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil.
authors.forename: Raquel
authors.initials: R
authors.lastname: Kummer
authors.affiliation: Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil.
authors.forename: Rafael Prizon
authors.initials: RP
authors.lastname: Tronco
authors.affiliation: Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil.
authors.forename: Roberto Kenji Nakamura
authors.initials: RKN
authors.lastname: Cuman
authors.affiliation: Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil. cabamado@uem.br.
authors.forename: Ciomar Aparecida
authors.initials: CA
authors.lastname: Bersani-Amado
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000188:drug therapy*; D002448:Cell Adhesion / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology*; D007596:Joints / Q000276:immunology / Q000473:pathology; D036904:Leukocyte Rolling / Q000187:drug effects; D007962:Leukocytes / Q000276:immunology; D007975:Leukotriene B4 / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D007975:Leukotriene B4; D009240:N-Formylmethionine Leucyl-Phenylalanine
keywords: arthritis; fish oil; leukocyte migration; microcirculation; time course