title: Applications of luminous bacteria enzymes in toxicology.
abstract: This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH:FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure.
authors.forename: Valentina A
authors.initials: VA
authors.lastname: Kratasyuk
authors.affiliation: Institute of Biophysics SB RAS, Akademgorodok 50/50, Krasnoyarsk, 660036, Russia. esimbekova@yandex.ru.
authors.forename: Elena N
authors.initials: EN
authors.lastname: Esimbekova
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D001681:Biological Assay / Q000639:trends; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D008163:Luminescent Measurements; D014116:Toxicology / Q000379:methods* / Q000639:trends*
chemical_list: D004800:Enzymes, Immobilized