title: Development of an acetylcholinesterase-choline oxidase based biosensor for acetylcholine determination.
abstract: In this article, a novel amperometric acetylcholine (ACh) biosensor with immobilization of acetylcholinesterase-choline oxidase (AChE-ChO) on electrochemically polymerized polyaniline-polyvinylsulphonate (PANI-PVS) film has been successfully developed for the first time. AChE and ChO enzymes were immobilized on PANI-PVS film by cross-linking with using glutaraldehyde (GA) and bovine serum albumin (BSA). Determination of ACh was carried out by oxidation of enzymatically produced H2O2 at +0.4 V versus Ag/AgCl. Optimum pH and temperature were found to be 8.0 and 60 °C, respectively. The linear part was in the region 1.0 × 10(-7)-6.0 × 10(-7) M (R(2) = 0.990). Storage stability and operation stability of the enzyme electrode were also studied.
authors.affiliation: a Department of Chemistry , Institute of Sciences, Gazi University , Ankara , Turkey.
authors.forename: Ayşe Tuğçe
authors.initials: AT
authors.lastname: Tunç
authors.affiliation: a Department of Chemistry , Institute of Sciences, Gazi University , Ankara , Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Aynacı Koyuncu
authors.affiliation: a Department of Chemistry , Institute of Sciences, Gazi University , Ankara , Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Arslan
mesh_terms: D000109:Acetylcholine / Q000032:analysis*; D000110:Acetylcholinesterase / Q000737:chemistry*; D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002417:Cattle; D004593:Electrophorus; D029941:Fish Proteins / Q000737:chemistry*
chemical_list: D029941:Fish Proteins; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D000110:Acetylcholinesterase; D000109:Acetylcholine
keywords: acetylcholine; acetylcholinesterase; biosensor; choline oxidase; polyaniline; polyvinylsulphonate