title: Chondrogenic potential of physically treated bovine cartilage matrix derived porous scaffolds on human dermal fibroblast cells.
abstract: Extracellular matrices have drawn attention in tissue engineering as potential biomaterials for scaffold fabrication because of their bioactive components. Noninvasive techniques of scaffold fabrication and cross-linking treatments are believed to maintain the integrity of bioactive molecules while providing proper architectural and mechanical properties. Cartilage matrix derived scaffolds are designed to support the maintenance of chondrocytes and provide proper signals for differentiation of chondroinducible cells. Chondroinductive potential of bovine articular cartilage matrix derived porous scaffolds on human dermal fibroblasts and the effect of scaffold shrinkage on chondrogenesis were investigated. An increase in sulfated glycosaminoglycans production along with upregulation of chondrogenic genes confirmed that physically treated cartilage matrix derived scaffolds have chondrogenic potential on human dermal fibroblasts.
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering Building, University of Malaya, Kuala Lumpur, 50603, Malaysia.|Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Moradi
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering Building, University of Malaya, Kuala Lumpur, 50603, Malaysia.
authors.forename: Forough
authors.initials: F
authors.lastname: Ataollahi
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering Building, University of Malaya, Kuala Lumpur, 50603, Malaysia.
authors.forename: Katayoun
authors.initials: K
authors.lastname: Sayar
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering Building, University of Malaya, Kuala Lumpur, 50603, Malaysia.
authors.forename: Sumit
authors.initials: S
authors.lastname: Pramanik
authors.affiliation: Department of Orthopedic Surgery, Faculty of Medicine Building, University of Malaya, Kuala Lumpur, 50603, Malaysia.
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Chong
authors.affiliation: Department of Surgery, Faculty of Medicine Building, University of Malaya, Kuala Lumpur, 50603, Malaysia.
authors.forename: Alizan Abdul
authors.initials: AA
authors.lastname: Khalil
authors.affiliation: Department of Orthopedic Surgery, Faculty of Medicine Building, University of Malaya, Kuala Lumpur, 50603, Malaysia.
authors.forename: Tunku
authors.initials: T
authors.lastname: Kamarul
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering Building, University of Malaya, Kuala Lumpur, 50603, Malaysia.
authors.forename: Belinda
authors.initials: B
authors.lastname: Pingguan-Murphy
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D002417:Cattle; D002454:Cell Differentiation / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D020219:Chondrogenesis / Q000528:radiation effects; D004247:DNA / Q000378:metabolism; D020405:Dermis / Q000166:cytology*; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects; D005786:Gene Expression Regulation; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D016062:Porosity; D013696:Temperature; D054457:Tissue Scaffolds / Q000737:chemistry*; D014466:Ultraviolet Rays; D014867:Water
chemical_list: D006025:Glycosaminoglycans; D014867:Water; D004247:DNA
keywords: cartilage matrix; chondrogenic; human dermal fibroblast; scaffold; shrinkage