title: Activation of Antibiotic Production in Bacillus spp. by Cumulative Drug Resistance Mutations.
abstract: Bacillus subtilis strains produce a wide range of antibiotics, including ribosomal and nonribosomal peptide antibiotics, as well as bacilysocin and neotrehalosadiamine. Mutations in B. subtilis strain 168 that conferred resistance to drugs such as streptomycin and rifampin resulted in overproduction of the dipeptide antibiotic bacilysin. Cumulative drug resistance mutations, such as mutations in the mthA and rpsL genes, which confer low- and high-level resistance, respectively, to streptomycin, and mutations in rpoB, which confer resistance to rifampin, resulted in cells that overproduced bacilysin. Transcriptional analysis demonstrated that the enhanced transcription of biosynthesis genes was responsible for the overproduction of bacilysin. This approach was effective also in activating the cryptic genes of Bacillus amyloliquefaciens, leading to actual production of antibiotic(s).
authors.affiliation: Hiroshima Institute of Technology, Department of Life Science, Hiroshima, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Tojo
authors.affiliation: Hiroshima Institute of Technology, Department of Life Science, Hiroshima, Japan.
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Hiroshima Institute of Technology, Department of Life Science, Hiroshima, Japan k.ochi.bz@it-hiroshima.ac.jp.
authors.forename: Kozo
authors.initials: K
authors.lastname: Ochi
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D001407:Bacillus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D004151:Dipeptides / Q000096:biosynthesis / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005959:Glucosidases / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics; D012293:Rifampin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D004151:Dipeptides; D012269:Ribosomal Proteins; C040343:ribosomal protein S19; C006674:bacilysin; D005959:Glucosidases; C099156:maltooligosyl trehalose trehalohydrolase; D012293:Rifampin