title: Spatial Localization and Binding of the Probiotic Lactobacillus farciminis to the Rat Intestinal Mucosa: Influence of Chronic Stress.
abstract: The present study aimed at detecting the exogenously applied probiotic Lactobacillus farciminis in rats, after exposure to IBS-like chronic stress, based on 4-day Water Avoidance Stress (WAS). The presence of L. farciminis in both ileal and colonic mucosal tissues was demonstrated by FISH and qPCR, with ileum as the preferential niche, as for the SFB population. A different spatial distribution of the probiotic was observed: in the ileum, bacteria were organized in micro-colonies more or less close to the epithelium whereas, in the colon, they were mainly visualized far away from the epithelium. When rats were submitted to WAS, the L. farciminis population substantially decreased in both intestinal regions, due to a stress-induced increase in colonic motility and defecation, rather than a modification of bacterial binding to the intestinal mucin Muc2.
authors.affiliation: Université de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France; UMR 1331 TOXALIM INRA/INPT/UPS, Equipe de NeuroGastroentérologie et Nutrition, 180 chemin de Tournefeuille, 31027 Toulouse cedex 9, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Da Silva
authors.affiliation: Univ Lille Nord de France, USTL, Unité de Glycobiologie Structurale et Fonctionnelle, IFR 147, F-59650 Villeneuve d'Ascq, France; CNRS, UMR 8576, F-59650 Villeneuve d'Ascq, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Robbe-Masselot
authors.affiliation: Université de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France.
authors.forename: Arthur
authors.initials: A
authors.lastname: Raymond
authors.affiliation: Université de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France.
authors.forename: Myriam
authors.initials: M
authors.lastname: Mercade-Loubière
authors.affiliation: UMR 1331 TOXALIM INRA/INPT/UPS, Equipe de NeuroGastroentérologie et Nutrition, 180 chemin de Tournefeuille, 31027 Toulouse cedex 9, France.
authors.forename: Christel
authors.initials: C
authors.lastname: Salvador-Cartier
authors.affiliation: Univ Lille Nord de France, USTL, Unité de Glycobiologie Structurale et Fonctionnelle, IFR 147, F-59650 Villeneuve d'Ascq, France; CNRS, UMR 8576, F-59650 Villeneuve d'Ascq, France.
authors.forename: Bélinda
authors.initials: B
authors.lastname: Ringot
authors.affiliation: Univ Lille Nord de France, USTL, Unité de Glycobiologie Structurale et Fonctionnelle, IFR 147, F-59650 Villeneuve d'Ascq, France; CNRS, UMR 8576, F-59650 Villeneuve d'Ascq, France.
authors.forename: Renaud
authors.initials: R
authors.lastname: Léonard
authors.affiliation: Faculté de Médecine Rangueil, Centre de Microscopie Electronique Appliquée à la Biologie (CMEAB), Bât. A5, 133 route de Narbonne, 31062 Toulouse cedex, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Fourquaux
authors.affiliation: UMR 1331 TOXALIM INRA/INPT/UPS, Equipe de NeuroGastroentérologie et Nutrition, 180 chemin de Tournefeuille, 31027 Toulouse cedex 9, France; Lallemand SA, 19 Rue des Briquetiers, 31702 Blagnac, France.
authors.forename: Afifa
authors.initials: A
authors.lastname: Ait-Belgnaoui
authors.affiliation: Université de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Loubière
authors.affiliation: UMR 1331 TOXALIM INRA/INPT/UPS, Equipe de NeuroGastroentérologie et Nutrition, 180 chemin de Tournefeuille, 31027 Toulouse cedex 9, France.
authors.forename: Vassilia
authors.initials: V
authors.lastname: Théodorou
authors.affiliation: Université de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France; UMR 1331 TOXALIM INRA/INPT/UPS, Equipe de NeuroGastroentérologie et Nutrition, 180 chemin de Tournefeuille, 31027 Toulouse cedex 9, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Mercier-Bonin
mesh_terms: D000818:Animals; D003106:Colon / Q000382:microbiology / Q000648:ultrastructure; D007082:Ileum / Q000382:microbiology / Q000648:ultrastructure; D007413:Intestinal Mucosa / Q000382:microbiology* / Q000648:ultrastructure; D007778:Lactobacillus / Q000472:pathogenicity*; D008297:Male; D055262:Mucin-2 / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000382:microbiology* / Q000473:pathology
chemical_list: C524628:Muc2 protein, rat; D055262:Mucin-2