title: Influence of feeding and earthworm density on compound bioaccumulation in earthworms Eisenia andrei.
abstract: Earthworm density and feeding during exposure to contaminated soil have been used inconsistently in bioaccumulation studies, which may lead to possible errors in risk assessment and modeling. Hydrophobic organic pollutants with a wide range of environmental properties (phenanthrene, pyrene, lindane, p,p'-DDT, and PCB 153) were used to study the effect of different earthworm densities in combination with the presence or absence of feeding on bioaccumulation factors (BAFs). Similar BAFs were found at various soil-to-worm ratios, with the exception of phenanthrene. We recommend using at least 15 gsoil dw per earthworm. The absence of feeding doubled the BAFs and, thus, using no food ration can be considered as "the worst case scenario". Whenever food is to be applied (i.e. to ensure the validity of the test in earthworm mass loss), we suggest feeding depending on the organic carbon content of the studied soil.
authors.affiliation: Masaryk University, Faculty of Science, RECETOX, 62500, Brno, Czech Republic. Electronic address: smidova@recetox.muni.cz.
authors.forename: Klára
authors.initials: K
authors.lastname: Šmídová
authors.affiliation: FT TBU, Dep. of Environmental Protection Engineering, 76001, Zlín, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Šerá
authors.affiliation: Masaryk University, Faculty of Science, RECETOX, 62500, Brno, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Bielská
authors.affiliation: Masaryk University, Faculty of Science, RECETOX, 62500, Brno, Czech Republic.
authors.forename: Jakub
authors.initials: J
authors.lastname: Hofman
mesh_terms: D000818:Animals; D003634:DDT / Q000493:pharmacokinetics; D005247:Feeding Behavior; D001556:Hexachlorocyclohexane / Q000493:pharmacokinetics; D009835:Oligochaeta / Q000378:metabolism* / Q000502:physiology; D010616:Phenanthrenes / Q000493:pharmacokinetics; D011078:Polychlorinated Biphenyls / Q000493:pharmacokinetics; D011156:Population Density; D011721:Pyrenes / Q000493:pharmacokinetics; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000493:pharmacokinetics*
chemical_list: D010616:Phenanthrenes; D011721:Pyrenes; D012987:Soil; D012989:Soil Pollutants; C031181:phenanthrene; D001556:Hexachlorocyclohexane; C030984:pyrene; D003634:DDT; D011078:Polychlorinated Biphenyls; C014024:2,4,5,2',4',5'-hexachlorobiphenyl
keywords: Aging; Artificial soil; Bioaccumulation factors; Bioavailability; Hydrophobic organic compounds