title: Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins.
abstract: Targeted protein degradation is a powerful tool in determining the function of specific proteins or protein complexes. We fused nanobodies to SPOP, an adaptor protein of the Cullin-RING E3 ubiquitin ligase complex, resulting in rapid ubiquitination and subsequent proteasome-dependent degradation of specific nuclear proteins in mammalian cells and zebrafish embryos. This approach is easily modifiable, as substrate specificity is conferred by an antibody domain that can be adapted to target virtually any protein.
authors.affiliation: Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chunchon, 200-701, Republic of Korea.
authors.forename: Yeong Ju
authors.initials: YJ
authors.lastname: Shin
authors.affiliation: Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chunchon, 200-701, Republic of Korea.
authors.forename: Seung Kyun
authors.initials: SK
authors.lastname: Park
authors.affiliation: Department of Systems Immunology, College of Biomedical Science, Kangwon National University, Chunchon, 200-701, Republic of Korea.
authors.forename: Yoo Jung
authors.initials: YJ
authors.lastname: Jung
authors.affiliation: Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chunchon, 200-701, Republic of Korea.
authors.forename: Ye Na
authors.initials: YN
authors.lastname: Kim
authors.affiliation: Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chunchon, 200-701, Republic of Korea.
authors.forename: Ki Sung
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Korea Basic Science Institute Chuncheon Center, Chuncheon, 200-701, Republic of Korea.
authors.forename: Ok Kyu
authors.initials: OK
authors.lastname: Park
authors.affiliation: Korea Basic Science Institute Chuncheon Center, Chuncheon, 200-701, Republic of Korea.
authors.forename: Seung-Hae
authors.initials: SH
authors.lastname: Kwon
authors.affiliation: Department of Life Science, Hallym University, Chuncheon, 200-702, Republic of Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Jeon
authors.affiliation: Biological Sciences and Biomedical Engineering, University of Southern California, Los Angeles, CA, USA.
authors.forename: Le A
authors.initials: le A
authors.lastname: Trinh
authors.affiliation: Biological Sciences and Biomedical Engineering, University of Southern California, Los Angeles, CA, USA.
authors.forename: Scott E
authors.initials: SE
authors.lastname: Fraser
authors.affiliation: Department of Systems Immunology, College of Biomedical Science, Kangwon National University, Chunchon, 200-701, Republic of Korea.
authors.forename: Yun
authors.initials: Y
authors.lastname: Kee
authors.affiliation: Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chunchon, 200-701, Republic of Korea.
authors.forename: Byung Joon
authors.initials: BJ
authors.lastname: Hwang
mesh_terms: D000818:Animals; D015870:Gene Expression; D017930:Genes, Reporter; D025743:HMGA2 Protein / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D059748:Proteolysis; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D061905:Single-Domain Antibodies / Q000276:immunology / Q000378:metabolism*; D043743:Ubiquitin-Protein Ligase Complexes / Q000276:immunology / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D054875:Ubiquitination; D015027:Zebrafish
chemical_list: D025743:HMGA2 Protein; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D061905:Single-Domain Antibodies; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases