title: [Treatment of Chemotherapy Related Leukocytopenia by Oral Administration of Multiple Leucogenic Drugs Combined with G-CSF: an Experimental Study].
abstract: OBJECTIVE
  To evaluate efficacies of three commonly used oral drugs including Berbamine Hydrochloride Tablet (B), Qijiao Shengbai Capsule (Q), and Leucogen Tablet (L) (by single drug, two drugs or three drugs) combined with granulocyte colony-stimulating factor (G-CSF) for treat ment of chemotherapy related leukocytopenia in mice.
  
  
  METHODS
  Totally 156 Kunming male mice were divided into the normal control group (A, n=24), the model group (B, n=24), the G-CSF group (C, n =24), the G-CSF+Q group (D, n=12), G-CSF+ B (E, n=12), the G-CSF+L group (F, n=12), the G-CSF + Q + B group (G, n=12), the G-CSF + Q + L group (H, n=12), the G-CSF + L + B group (I, n=12), and the G-CSF + L + Q + B (J, n=12). Mouse models of chemotherapy related leukocytopenia were established by intraperitoneal injection of cyclophosphamide (CTX). A G-CSF group was set up as a positive control. Mice were treated by a single oral drug, a single oral drug combined with G-CSF, and two or three drugs combined with G-CSF respectively, and the death rate calculated. Hemocytes [such as white blood cells (WBC) and its classification, red blood cells (RBC), platelet (PLT), hemoglobin (Hb)] were calculated by hematology analyzer. Mice were anatomized and important organs weighed. Organ indices were calculated.
  
  
  RESULTS
  There was no statistical difference in the mortality rate among all groups (P > 0.05). Compared with Group B, WBC was elevated in all other groups (P < 0.01). WBC and PLT were elevated most in Group J, Hb and RBC were also increased at the same time (P < 0.05, P < 0. 01). Compared with Group B, RBC increased in Group E, F, G, I, and J (P < 0.01); Hb obviously increased in Group C, E, F, H, I, and J (P<0.01). Compared with Group B and D, the promotion of erythroid hematopoiesis by G-CSF could be elevated in any group contained drug B and L (P < 0.05, P < 0.01). The spleen index of model mice could be significantly improved in Group C, D, and G (P < 0.01). The thymus index of model mice could be significantly improved in Group H (P < 0.05).
  
  
  CONCLUSIONS
  The best scheme to treat mice with chemotherapy related leukopenia or decreased three blood series was to administrate three commonly oral drugs combined with G-CSF. Authors speculated that G-CSF and Q might have a certain effect on CTX induced immune inhibition.
authors.forename: Xi-ping
authors.initials: XP
authors.lastname: Zhang
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Hong-jian
authors.initials: HJ
authors.lastname: Yang
authors.forename: De-hong
authors.initials: DH
authors.lastname: Zou
authors.forename: Xiang-ming
authors.initials: XM
authors.lastname: He
authors.forename: Xing-fei
authors.initials: XF
authors.lastname: Yu
authors.forename: Yong-feng
authors.initials: YF
authors.lastname: Li
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001792:Blood Platelets; D003520:Cyclophosphamide; D064420:Drug-Related Side Effects and Adverse Reactions / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004906:Erythrocyte Count; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D006410:Hematopoiesis; D006454:Hemoglobins; D007958:Leukocyte Count; D007962:Leukocytes; D007970:Leukopenia / Q000139:chemically induced* / Q000188:drug therapy; D008297:Male; D051379:Mice; D004364:Pharmaceutical Preparations
chemical_list: D004365:Drugs, Chinese Herbal; D006454:Hemoglobins; D004364:Pharmaceutical Preparations; D016179:Granulocyte Colony-Stimulating Factor; D003520:Cyclophosphamide