title: Production of Long-Chain α,ω-Dicarboxylic Acids by Engineered Escherichia coli from Renewable Fatty Acids and Plant Oils.
abstract: Long-chain α,ω-dicarboxylic acids (LDCAs, ≥ C12) are widely used as a raw material for preparing various commodities and polymers. In this study, a CYP450-monooxygenase-mediated ω-oxidation pathway system with high ω-regioselectivity was heterologously expressed in Escherichia coli to produce DCAs from fatty acids. The resulting engineered E. coli produced a maximum of 41 mg/L of C12 DCA and 163 mg/L of C14 DCA from fatty acids (1 g/L), following 20 h of whole cell biotransformation. Addition of a heme precursor and the hydroxyl radical scavenger, thiourea, increased product concentration (159 mg/L of C12 DCA and 410 mg/L of C14 DCA) in a shorter culture duration than that of the corresponding controls. DCAs of various chain lengths were synthesized from coconut oil hydrolysate using the engineered E. coli. This novel synthetic biocatalytic system could be applied to produce high value DCAs in a cost-effective manner from renewable plant oils.
authors.affiliation: School of Energy and Chemical Engineering, and ‡School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST) , Ulsan 689-798, Republic of Korea.
authors.forename: Chandran
authors.initials: C
authors.lastname: Sathesh-Prabu
authors.affiliation: School of Energy and Chemical Engineering, and ‡School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST) , Ulsan 689-798, Republic of Korea.
authors.forename: Sung Kuk
authors.initials: SK
authors.lastname: Lee
mesh_terms: D000622:Aminolevulinic Acid / Q000494:pharmacology; D003001:Cloning, Molecular; D000074263:Coconut Oil; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D003998:Dicarboxylic Acids / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D015870:Gene Expression; D006861:Hydrogen Peroxide / Q000378:metabolism; D010084:Oxidation-Reduction; D010938:Plant Oils / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013890:Thiourea / Q000494:pharmacology
chemical_list: D003998:Dicarboxylic Acids; D005227:Fatty Acids; D010938:Plant Oils; D011994:Recombinant Proteins; D000622:Aminolevulinic Acid; D003577:Cytochrome P-450 Enzyme System; D006861:Hydrogen Peroxide; D013890:Thiourea; D000074263:Coconut Oil
keywords: CYP450s; fatty acids; monooxygenase; α,ω-dicarboxylic acids; ω-oxidation pathway