title: Real-time reverse transcriptase polymerase chain reaction assays for Middle East Respiratory Syndrome.
abstract: Here we designed and tested two highly specific quantitative TaqMan(®)-MGB-based reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays for Middle East Respiratory Syndrome (MERS). The primers and probes for these assays were evaluated and found to have a limit of detection (LOD) of 0.005 plaque-forming units/PCR (pfu/PCR).
authors.affiliation: Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA.
authors.forename: Christina E
authors.initials: CE
authors.lastname: Douglas
authors.affiliation: Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Kulesh
authors.affiliation: Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA.
authors.forename: James G
authors.initials: JG
authors.lastname: Jaissle
authors.affiliation: Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA. Electronic address: timothy.d.minogue.civ@mail.mil.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Minogue
mesh_terms: D017934:Coronavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000175:diagnosis* / Q000821:virology; D017931:DNA Primers / Q000032:analysis; D015342:DNA Probes / Q000032:analysis; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods; D012367:RNA, Viral / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D012367:RNA, Viral
keywords: Coronavirus; MERS; RT-qPCR; TaqMan(®)-MGB