title: PML-RARa modulates the vascular signature of extracellular vesicles released by acute promyelocytic leukemia cells.
abstract: Oncogenic transformation is believed to impact the vascular phenotype and microenvironment in cancer, at least in part, through mechanisms involving extracellular vesicles (EVs). We explored these questions in the context of acute promyelocytic leukemia cells (NB4) expressing oncogenic fusion protein, PML-RARa and exquisitely sensitive to its clinically used antagonist, the all-trans retinoic acid (ATRA). We report that NB4 cells produce considerable numbers of EVs, which are readily taken up by cultured endothelial cells triggering their increased survival. NB4 EVs contain PML-RARa transcript, but no detectable protein, which is also absent in endothelial cells upon the vesicle uptake, thereby precluding an active intercellular trafficking of this oncogene in this setting. ATRA treatment changes the emission profile of NB4-related EVs resulting in preponderance of smaller vesicles, an effect that occurs in parallel with the onset of cellular differentiation. ATRA also increases IL-8 mRNA and protein content in NB4 cells and their EVs, while decreasing the levels of VEGF and tissue factor (TF). Endothelial cell uptake of NB4-derived EVs renders these cells more TF-positive and procoagulant, and this effect is diminished by pre-treatment of EV donor cells with ATRA. Profiling angiogenesis-related transcripts in intact and ATRA-treated APL cells and their EVs reveals multiple differences attributable to cellular responses and EV molecular packaging. These observations point to the potential significance of changes in the angiogenic signature and activity associated with EVs released from tumor cells subjected to targeted therapy.
authors.affiliation: The Research Institute of the McGill University Health Centre, Montreal Children's Hospital, McGill University, 1001 Decarie Blvd, Montreal, QC, H4A 3J1, Canada.|Department of Hematology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Fang
authors.affiliation: The Research Institute of the McGill University Health Centre, Montreal Children's Hospital, McGill University, 1001 Decarie Blvd, Montreal, QC, H4A 3J1, Canada.
authors.forename: Delphine
authors.initials: D
authors.lastname: Garnier
authors.affiliation: The Research Institute of the McGill University Health Centre, Montreal Children's Hospital, McGill University, 1001 Decarie Blvd, Montreal, QC, H4A 3J1, Canada.
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Lee
authors.affiliation: The Research Institute of the McGill University Health Centre, Montreal Children's Hospital, McGill University, 1001 Decarie Blvd, Montreal, QC, H4A 3J1, Canada.
authors.forename: Esterina
authors.initials: E
authors.lastname: D'Asti
authors.affiliation: The Research Institute of the McGill University Health Centre, Montreal Children's Hospital, McGill University, 1001 Decarie Blvd, Montreal, QC, H4A 3J1, Canada.
authors.forename: Laura
authors.initials: L
authors.lastname: Montermini
authors.affiliation: The Research Institute of the McGill University Health Centre, Montreal Children's Hospital, McGill University, 1001 Decarie Blvd, Montreal, QC, H4A 3J1, Canada.
authors.forename: Brian
authors.initials: B
authors.lastname: Meehan
authors.affiliation: The Research Institute of the McGill University Health Centre, Montreal Children's Hospital, McGill University, 1001 Decarie Blvd, Montreal, QC, H4A 3J1, Canada. janusz.rak@mcgill.ca.
authors.forename: Janusz
authors.initials: J
authors.lastname: Rak
mesh_terms: D001777:Blood Coagulation / Q000187:drug effects; D045744:Cell Line, Tumor; D000067128:Extracellular Vesicles / Q000187:drug effects / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000473:pathology*; D015514:Oncogene Proteins, Fusion / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013925:Thromboplastin / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D015514:Oncogene Proteins, Fusion; D012333:RNA, Messenger; C093476:promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein; D014212:Tretinoin; D013925:Thromboplastin
keywords: ATRA; Acute promyelocytic leukemia; Angiogenesis; Angiogenic signature; Cancer biomarker; Endothelial cells; Exosomes; Extracellular RNA; Extracellular vesicles; Oncogene; PML–RARa; Targeted agents; Tissue factor