title: De novo assembly of the mitochondrial genome of Ulva fasciata Delile (Ulvophyceae, Chlorophyta), a distromatic blade-forming green macroalga.
abstract: We present the 61 614 bp circular-mapping mitochondrial genome of Ulva fasciata. Fifty-eight genes were identified including 29 protein-coding genes, 27 transfer RNA (tRNA) genes, and two ribosomal RNA (rRNA) genes. Four ORFs from the Ulva sp. UNA00071828 mitogenome were conserved compared to the mitogenome of Ulva fasciata. The ∼10 000 bp size difference was mostly due to fewer introns found in U. fasciata mtDNA (4) as compared with the Ulva sp. UNA00071828 mtDNA (10). Introns were annotated in cox1 (3) and nad3 (1). The AT content was similar to Ulva sp. UNA00071828 mtDNA at 67.5%. A phylogenomic anaylsis of 28 chlorophytes and 17 protein-coding genes (14 115 bp alignment) showed that U. fasciata clusters closely with other members of the Ulvophyceae (Ulva sp. UNA00071828, Pseudendoclonium akinetum, and Oltmannsiellopsis viridis). This analysis did not recover a monophyletic Ulvophyceae, however, the Trebouxiophyceae and Chlorophyceae were supported as monophyletic groups.
authors.affiliation: a Department of Biological Sciences , The University of Alabama , Tuscaloosa , AL , USA.
authors.forename: James Theodore
authors.initials: JT
authors.lastname: Melton
authors.affiliation: a Department of Biological Sciences , The University of Alabama , Tuscaloosa , AL , USA.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Lopez-Bautista
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D017124:Conserved Sequence; D004272:DNA, Mitochondrial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D020459:Genes, rRNA; D054629:Genome, Mitochondrial; D018745:Genome, Plant; D007438:Introns; D010940:Plant Proteins / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D044445:Ulva / Q000235:genetics*; D000073336:Whole Genome Sequencing
chemical_list: D004272:DNA, Mitochondrial; D018744:DNA, Plant; D010940:Plant Proteins; D012343:RNA, Transfer
keywords: Green algae; Ulva; Ulvaceae; Ulvales; marine; mitochondrion