title: Prevailing Sydney like Norovirus GII.4 VLPs induce systemic and mucosal immune responses in mice.
abstract: The newly emerged Norovirus (NoV) Sydney 2012 strain has been sweeping all over the world, causing acute non-bacterial gastroenteritis in adults and children. Due to a lack of cell culture system, virus like particles (VLPs) has been assembled and used as vaccine candidates in preclinical and clinical studies. Expression of the major capsid protein of NoVs using recombinant baculovirus expression system in Sf9 cells leads to formation of VLPs that are morphologically and antigenically similar to true virions. In this study, VLPs were successfully produced using the VP1 of Sydney-2012-like strain and its immunogenicity was evaluated by different routes and its capability in inducing mucosal immune responses in the presence and absence of adjuvants in BALB/c mice. Administration of NoV VLPs in the presence of Al(OH)3 or monophosphoryl lipid A (MPL-A) led to high titers of VLP-specific IgG antibodies. Administration of VLPs orally in the presence of cholera toxin subunit B (CTB) didn't enhance mucosal immune response as less fecal IgA positive mice were observed when compared with those given VLPs only. Our study represents the first immunogenicity study of VLPs derived from current pandemic Sydney 2012 strain and which might have implications in the development of NoVs vaccine in china.
authors.affiliation: Wuhan Institute of Biological Products Co., Ltd., Wuhan, PR China. Electronic address: 1246105971@qq.com.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Huo
authors.affiliation: Wuhan Institute of Biological Products Co., Ltd., Wuhan, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wan
authors.affiliation: Wuhan Institute of Biological Products Co., Ltd., Wuhan, PR China.
authors.forename: Tong
authors.initials: T
authors.lastname: Ling
authors.affiliation: Wuhan Institute of Biological Products Co., Ltd., Wuhan, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.affiliation: Wuhan Institute of Biological Products Co., Ltd., Wuhan, PR China.
authors.forename: Zejun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Wuhan Institute of Biological Products Co., Ltd., Wuhan, PR China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Meng
authors.affiliation: Wuhan Institute of Biological Products Co., Ltd., Wuhan, PR China. Electronic address: shenshuo1@sinopharm.com.
authors.forename: Shuo
authors.initials: S
authors.lastname: Shen
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000534:Alum Compounds / Q000008:administration & dosage; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D016367:Baculoviridae / Q000235:genetics; D017250:Caliciviridae Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D002772:Cholera Toxin / Q000008:administration & dosage; D005260:Female; D015870:Gene Expression; D006801:Humans; D018928:Immunity, Mucosal / Q000187:drug effects*; D007114:Immunization; D008050:Lipid A / Q000008:administration & dosage / Q000031:analogs & derivatives; D051379:Mice; D008807:Mice, Inbred BALB C; D029322:Norovirus / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D061987:Sf9 Cells; D018411:Spodoptera; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014771:Virion / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000914:Antibodies, Viral; D008050:Lipid A; D011994:Recombinant Proteins; D058425:Vaccines, Virus-Like Particle; D014765:Viral Vaccines; C041524:aluminum sulfate; D002772:Cholera Toxin; C048436:monophosphoryl lipid A
keywords: Adjuvants; Immune response; Norovirus; Sydney-2012-like strain