title: The Arabidopsis Transcription Factor MYB112 Promotes Anthocyanin Formation during Salinity and under High Light Stress.
abstract: MYB transcription factors (TFs) are important regulators of flavonoid biosynthesis in plants. Here, we report MYB112 as a formerly unknown regulator of anthocyanin accumulation in Arabidopsis (Arabidopsis thaliana). Expression profiling after chemically induced overexpression of MYB112 identified 28 up- and 28 down-regulated genes 5 h after inducer treatment, including MYB7 and MYB32, which are both induced. In addition, upon extended induction, MYB112 also positively affects the expression of PRODUCTION OF ANTHOCYANIN PIGMENT1, a key TF of anthocyanin biosynthesis, but acts negatively toward MYB12 and MYB111, which both control flavonol biosynthesis. MYB112 binds to an 8-bp DNA fragment containing the core sequence (A/T/G)(A/C)CC(A/T)(A/G/T)(A/C)(T/C). By electrophoretic mobility shift assay and chromatin immunoprecipitation coupled to quantitative polymerase chain reaction, we show that MYB112 binds in vitro and in vivo to MYB7 and MYB32 promoters, revealing them as direct downstream target genes. We further show that MYB112 expression is up-regulated by salinity and high light stress, environmental parameters that both require the MYB112 TF for anthocyanin accumulation under these stresses. In contrast to several other MYB TFs affecting anthocyanin biosynthesis, MYB112 expression is not controlled by nitrogen limitation or an excess of carbon. Thus, MYB112 constitutes a regulator that promotes anthocyanin accumulation under abiotic stress conditions.
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, D-14476 Potsdam-Golm, Germany (M.E.L., S.B., B.M.-R.);Central Metabolism (T.T., A.R.F.) andPlant Signaling (S.B., B.M.-R.) Groups, Max-Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany; andCSIRO Agriculture Flagship, Brisbane, Queensland 4067, Australia (G.-P.X.).
authors.forename: Magda E
authors.initials: ME
authors.lastname: Lotkowska
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, D-14476 Potsdam-Golm, Germany (M.E.L., S.B., B.M.-R.);Central Metabolism (T.T., A.R.F.) andPlant Signaling (S.B., B.M.-R.) Groups, Max-Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany; andCSIRO Agriculture Flagship, Brisbane, Queensland 4067, Australia (G.-P.X.).
authors.forename: Takayuki
authors.initials: T
authors.lastname: Tohge
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, D-14476 Potsdam-Golm, Germany (M.E.L., S.B., B.M.-R.);Central Metabolism (T.T., A.R.F.) andPlant Signaling (S.B., B.M.-R.) Groups, Max-Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany; andCSIRO Agriculture Flagship, Brisbane, Queensland 4067, Australia (G.-P.X.).
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, D-14476 Potsdam-Golm, Germany (M.E.L., S.B., B.M.-R.);Central Metabolism (T.T., A.R.F.) andPlant Signaling (S.B., B.M.-R.) Groups, Max-Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany; andCSIRO Agriculture Flagship, Brisbane, Queensland 4067, Australia (G.-P.X.).
authors.forename: Gang-Ping
authors.initials: GP
authors.lastname: Xue
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, D-14476 Potsdam-Golm, Germany (M.E.L., S.B., B.M.-R.);Central Metabolism (T.T., A.R.F.) andPlant Signaling (S.B., B.M.-R.) Groups, Max-Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany; andCSIRO Agriculture Flagship, Brisbane, Queensland 4067, Australia (G.-P.X.).
authors.forename: Salma
authors.identifier: http://orcid.org/0000-0002-5789-4071
authors.initials: S
authors.lastname: Balazadeh
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, D-14476 Potsdam-Golm, Germany (M.E.L., S.B., B.M.-R.);Central Metabolism (T.T., A.R.F.) andPlant Signaling (S.B., B.M.-R.) Groups, Max-Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany; andCSIRO Agriculture Flagship, Brisbane, Queensland 4067, Australia (G.-P.X.) bmr@uni-potsdam.de.
authors.forename: Bernd
authors.identifier: http://orcid.org/0000-0002-1410-464X
authors.initials: B
authors.lastname: Mueller-Roeber
mesh_terms: D000872:Anthocyanins / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D008027:Light; D011401:Promoter Regions, Genetic / Q000235:genetics; D054712:Salinity; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000872:Anthocyanins; D029681:Arabidopsis Proteins; C000611118:MYB112 protein, Arabidopsis; D014157:Transcription Factors