title: Spirally-patterned pinhole arrays for long-term fluorescence cell imaging.
abstract: Fluorescence cell imaging using a fluorescence microscope is an extensively used technique to examine the cell nucleus, internal structures, and other cellular molecules with fluorescence response time and intensity. However, it is difficult to perform high resolution cell imaging for a long period of time with this technique due to necrosis and apoptosis depending on the type and subcellular location of the damage caused by phototoxicity. A large number of studies have been performed to resolve this problem, but researchers have struggled to meet the challenge between cellular viability and image resolution. In this study, we employ a specially designed disc to reduce cell damage by controlling total fluorescence exposure time without deterioration of the image resolution. This approach has many advantages such as, the apparatus is simple, cost-effective, and easily integrated into the optical pathway through a conventional fluorescence microscope.
authors.affiliation: Department of Mechanical Engineering, College of Engineering, Kyung Hee University, 1732 Deokyoungdaero, Giheung, Yongin 446-701, Republic of Korea. termylee@khu.ac.kr.
authors.forename: Bon Ung
authors.initials: BU
authors.lastname: Koo
authors.forename: YooNa
authors.initials: Y
authors.lastname: Kang
authors.forename: SangJun
authors.initials: S
authors.lastname: Moon
authors.forename: Won Gu
authors.initials: WG
authors.lastname: Lee
mesh_terms: D017209:Apoptosis; D002452:Cell Count; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival; D003362:Cost-Benefit Analysis; D004867:Equipment Design; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D009336:Necrosis; D014466:Ultraviolet Rays
chemical_list: D005456:Fluorescent Dyes