title: Lack of haplotype structuring for two candidate genes for trypanotolerance in cattle.
abstract: Bovine trypanotolerance is a heritable trait associated to the ability of the individuals to control parasitaemia and anaemia. The INHBA (BTA4) and TICAM1 (BTA7) genes are strong candidates for trypanotolerance-related traits. The coding sequence of both genes (3951 bp in total) were analysed in a panel including 79 Asian, African and European cattle (Bos taurus and B. indicus) to identify naturally occurring polymorphisms on both genes. In general, the genetic diversity was low. Nineteen of the 33 mutations identified were found just one time. Seventeen different haplotypes were defined for the TICAM1 gene, and 9 and 12 were defined for the exon 1 and the exon 2 of the INHBA gene, respectively. There was no clear separation between cattle groups. The most frequent haplotypes identified in West African taurine samples were also identified in other cattle groups including Asian zebu and European cattle. Phylogenetic trees and principal component analysis confirmed that divergence among the cattle groups analysed was poor, particularly for the INHBA sequences. The European cattle subset had the lowest values of haplotype diversity for both the exon1 (monomorphic) and the exon2 (0.077 ± 0.066) of the INHBA gene. Neutrality tests, in general, did not suggest that the analysed genes were under positive selection. The assessed scenario would be consistent with the identification of recent mutations in evolutionary terms.
authors.affiliation: Área de Genética y Reproducción Animal, SERIDA, Gijón, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Álvarez
authors.affiliation: CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Vairão, Portugal.
authors.forename: L
authors.initials: L
authors.lastname: Pérez-Pardal
authors.affiliation: INERA, Ouagadougou, Burkina Faso.
authors.forename: A
authors.initials: A
authors.lastname: Traoré
authors.affiliation: Área de Genética y Reproducción Animal, SERIDA, Gijón, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Fernández
authors.affiliation: Área de Genética y Reproducción Animal, SERIDA, Gijón, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Goyache
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics* / Q000276:immunology; D006239:Haplotypes; D010802:Phylogeny; D025341:Principal Component Analysis; D014353:Trypanosomiasis, African / Q000235:genetics / Q000276:immunology / Q000662:veterinary*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport
keywords: Bos indicus; Bos taurus; INHBA; TICAM1; West Africa; trypanotolerance