title: Facile and selective covalent grafting of an RGD-peptide to electrospun scaffolds improves HUVEC adhesion.
abstract: The development of a biomimetic surface able to promote endothelialization is fundamental in the search for blood vessel substitutes that prevent the formation of thrombi or hyperplasia. This study aims at investigating the effect of functionalization of poly-ε-caprolactone or poly(L-lactic acid-co-ɛ-caprolactone) electrospun scaffolds with a photoreactive adhesive peptide. The designed peptide sequence contains four Gly-Arg-Gly-Asp-Ser-Pro motifs per chain and a p-azido-Phe residue at each terminus. Different peptide densities on the scaffold surface were obtained by simply modifying the peptide concentration used in pretreatment of the scaffold before UV irradiation. Scaffolds of poly-ε-caprolactone embedded with adhesive peptides were produced to assess the importance of peptide covalent grafting. Our results show that the scaffolds functionalized with photoreactive peptides enhance adhesion at 24 h with a dose-dependent effect and control the proliferation of human umbilical vein endothelial cells, whereas the inclusion of adhesive peptide in the electrospun matrices by embedding does not give satisfactory results.
authors.affiliation: Department of Industrial Engineering, University of Padova, Padova, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Dettin
authors.affiliation: Department of Industrial Engineering, University of Padova, Padova, Italy.
authors.forename: Annj
authors.initials: A
authors.lastname: Zamuner
authors.affiliation: Department of Industrial Engineering, University of Padova, Padova, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Roso
authors.affiliation: Department of Physics, University Roma Tre, Rome, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Iucci
authors.affiliation: Inter-university Centre for Materials Research and Engineering, University Toulouse-Paul Sabatier, Toulouse, France.
authors.forename: Valerie
authors.initials: V
authors.lastname: Samouillan
authors.affiliation: Department of Industrial Engineering, University of Padova, Padova, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Danesin
authors.affiliation: Department of Industrial Engineering, University of Padova, Padova, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Modesti
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Conconi
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D009842:Oligopeptides / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid
keywords: HUVEC; biomaterials; covalent grafting; peptides; poly(l-lactic acid-co-ɛ-caprolactone); poly-ε-caprolactone