title: ATP selection in a random peptide library consisting of prebiotic amino acids.
abstract: Based upon many theoretical findings on protein evolution, we proposed a ligand-selection model for the origin of proteins, in which the most ancient proteins originated from ATP selection in a pool of random peptides. To test this ligand-selection model, we constructed a random peptide library consisting of 15 types of prebiotic amino acids and then used cDNA display to perform six rounds of in vitro selection with ATP. By means of next-generation sequencing, the most prevalent sequence was defined. Biochemical and biophysical characterization of the selected peptide showed that it was stable and foldable and had ATP-hydrolysis activity as well.
authors.affiliation: Agricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Shou-Kai
authors.initials: SK
authors.lastname: Kang
authors.affiliation: Agricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Bai-Xue
authors.initials: BX
authors.lastname: Chen
authors.affiliation: Agricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.affiliation: Agricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Xi-Shuai
authors.initials: XS
authors.lastname: Jia
authors.affiliation: Agricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Xin-Yi
authors.initials: XY
authors.lastname: Chu
authors.affiliation: Agricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.affiliation: Agricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Dong
authors.affiliation: Agricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Qing-Yong
authors.initials: QY
authors.lastname: Yang
authors.affiliation: Agricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China. Electronic address: zhy630@mail.hzau.edu.cn.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Zhang
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D000596:Amino Acids / Q000737:chemistry*; D019295:Computational Biology; D018076:DNA, Complementary / Q000378:metabolism; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D006868:Hydrolysis; D008024:Ligands; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D056692:Prebiotics; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D000596:Amino Acids; D018076:DNA, Complementary; D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D056692:Prebiotics; D011506:Proteins; D012333:RNA, Messenger; D000255:Adenosine Triphosphate
keywords: In vitro selection; Next-generation sequencing; Origin of proteins; Protein evolution; Structure and function; cDNA display