title: Effect of pannexin-1 on the release of glutamate and cytokines in astrocytes.
abstract: We investigated the effect of pannexin-1 (PANX-1) on the release of glutamate and cytokines in U87 human malignant glioma (U87-MG) cells using small interfering RNA (siRNA) transfection and adenosine triphosphate/lipopolysaccharide treatment. PANX-1 is a new family member of the gap junction proteins, and is permeable to ions, metabolic molecules, second messengers, and neurotransmitters, among other factors. PANX-1 plays an important role in the regulation of cell inflammation. However, the relationship between PANX-1 and the secretion of glutamate, interleukin (IL)-6 and IL-8 in astrocytes is still poorly understood. We found that the levels of IL-6, IL-8 and glutamate were lower in PANX-1 siRNA transfected cells, while the levels of IL-6, IL-8 and glutamate were unaltered in cells without changes in the expression of PANX-1 protein. This provides further support for the hypothesis that PANX-1 plays an important role in the release of IL-6, IL-8 and glutamate in U87-MG cells.
authors.affiliation: Department of Neurology, Jinshan Hospital, Fudan University, Shanghai 201508, PR China; Department of Neurology, Shanghai Medical College, Fudan University, Shanghai, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Neurology, Jinshan Hospital, Fudan University, Shanghai 201508, PR China.
authors.forename: Hanmiao
authors.initials: H
authors.lastname: Sheng
authors.affiliation: Department of Neurology, Jinshan Hospital, Fudan University, Shanghai 201508, PR China; Department of Neurology, Shanghai Medical College, Fudan University, Shanghai, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Shanghai Jiaotong University, Shanghai 201508, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Hao
authors.affiliation: Department of Endocrinology, Jinshan Hospital, Fudan University, Shanghai, PR China. Electronic address: shixh80301@163.com.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Neurology, Jinshan Hospital, Fudan University, Shanghai 201508, PR China; Department of Neurology, Shanghai Medical College, Fudan University, Shanghai, PR China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D045744:Cell Line, Tumor; D017630:Connexins / Q000172:deficiency* / Q000235:genetics; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000172:deficiency* / Q000235:genetics; D000093242:Interleukin-6 Inhibitors
chemical_list: D000255:Adenosine Triphosphate; D017630:Connexins; D016207:Cytokines; D018698:Glutamic Acid; D015850:Interleukin-6; D009419:Nerve Tissue Proteins; D000093242:Interleukin-6 Inhibitors; C508600:IL6 protein, human; C492764:PANX1 protein, human
keywords: Astrocytes; Cytokine; Gap junction; Glutamate; Pannexin-1