title: Ultrasound mediated destruction of multifunctional microbubbles for image guided delivery of oxygen and drugs.
abstract: We synthesized multifunctional activatible microbubbles (MAMs) for ultrasound mediated delivery of oxygen and drugs with both ultrasound and fluorescence imaging guidance. Oxygen enriched perfluorocarbon (PFC) compound was encapsulated in liposome microbubbles (MBs) by a modified emulsification process. DiI dye was loaded as a model drug. The ultrasound targeted microbubble destruction (UTMD) process was guided by both ultrasonography and fluorescence imaging modalities. The process was validated in both a dialysis membrane tube model and a porcine carotid artery model. Our experiment results show that the UTMD process effectively facilitates the controlled delivery of oxygen and drug at the disease site and that the MAM agent enables ultrasound and fluorescence imaging guidance of the UTMD process. The proposed MAM agent can be potentially used for UTMD-mediated combination therapy in hypoxic ovarian cancer.
authors.affiliation: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA; Department of Obstetrics and Gynecology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Chang
authors.affiliation: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA; College of Engineering, University of Science and Technology of China, Hefei, Anhui 230027, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Si
authors.affiliation: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA; College of Engineering, University of Science and Technology of China, Hefei, Anhui 230027, China.
authors.forename: Shiwu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Division of Health & Rehabilitation Sciences, The Ohio State University Medical Center, Columbus, OH 43210, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Merrick
authors.affiliation: Department of Obstetrics and Gynecology, The Ohio State University Medical Center, Columbus, OH 43210, USA.
authors.forename: David E
authors.initials: DE
authors.lastname: Cohn
authors.affiliation: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA; College of Engineering, University of Science and Technology of China, Hefei, Anhui 230027, China. Electronic address: xu.ronald@gmail.com.
authors.forename: Ronald X
authors.initials: RX
authors.lastname: Xu
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000378:metabolism; D002339:Carotid Arteries / Q000000981:diagnostic imaging / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D045423:Microbubbles; D061848:Optical Imaging; D010100:Oxygen / Q000378:metabolism*; D019047:Phantoms, Imaging; D013552:Swine; D014463:Ultrasonography
chemical_list: D002232:Carbocyanines; C024286:3,3'-dioctadecylindocarbocyanine; D010100:Oxygen
keywords: Drug delivery; Fluorescence imaging; Multifunctional microbubbles; Oxygen; Perfluorocarbon; Ultrasound targeted microbubble destruction