title: A simple method for the addition of rotenone in Arabidopsis thaliana leaves.
abstract: A simple and reproducible method for the treatment of Arabidopsis thaliana leaves with rotenone is presented. Rosette leaves were incubated with rotenone and Triton X-100 for at least 15 h. Treated leaves showed increased expression of COX19 and BCS1a, 2 genes known to be induced in Arabidopsis cell cultures after rotenone treatment. Moreover, rotenone/Triton X-100 incubated leaves presented an inhibition of oxygen uptake. The simplicity of the procedure shows this methodology is useful for studying the effect of the addition of rotenone to a photosynthetic tissue in situ.
authors.affiliation: a IIB- INTECH; Universidad Nacional de General San Martín (UNSAM) ; Chascomús, Buenos Aires , Argentina.
authors.forename: María V
authors.initials: MV
authors.lastname: Maliandi
authors.affiliation: b Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET); Universidad Nacional de Rosario ; Rosario , Argentina.
authors.forename: Sebastián P
authors.initials: SP
authors.lastname: Rius
authors.affiliation: b Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET); Universidad Nacional de Rosario ; Rosario , Argentina.
authors.forename: María V
authors.initials: MV
authors.lastname: Busi
authors.affiliation: b Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET); Universidad Nacional de Rosario ; Rosario , Argentina.
authors.forename: Diego F
authors.initials: DF
authors.lastname: Gomez-Casati
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism; D001901:Botany; D003585:Cell Biology; D018929:Cell Culture Techniques; D042967:Electron Transport Complex I / Q000378:metabolism; D017343:Genes, Plant; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism*; D017830:Octoxynol; D010100:Oxygen / Q000378:metabolism*; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects* / Q000378:metabolism; D012402:Rotenone / Q000494:pharmacology*
chemical_list: D029681:Arabidopsis Proteins; D024101:Mitochondrial Proteins; C000607130:OM66 protein, Arabidopsis; D012402:Rotenone; D017830:Octoxynol; D042967:Electron Transport Complex I; D010100:Oxygen
keywords: Arabidopsis; complex I; mitochondria; mitochondrial respiration; rotenone