title: Tob1 enhances radiosensitivity of breast cancer cells involving the JNK and p38 pathways.
abstract: The aim of the present study was to evaluate the effect of Tob1 on the radiosensitivity of breast cancer cells. The results showed that overexpression of Tob1 reduced the clonogenic growth of 231 cells and induced the rate of apoptosis. Tob1 caused an accumulation of cells in the G0 /G1 phase and decreased the percentage of cells in S phase. We also found that overexpression of Tob1 significantly reduced the phosphorylation of JNK and p38. The activator of JNK and p38, anisomycin, attenuated the blockage of Tob1 on the cell cycle and reversed the effect of Tob1 on apoptosis. Taken together, Tob1 enhanced radiosensitivity of breast cancer cells through regulation of the JNK and p38 pathways. The results indicated that Tob1 might be a promising molecular in gene therapy for the treatment of breast cancer.
authors.affiliation: Department of Radiotherapy, Huaihe Hospital of Henan University, Kaifeng, Henan, 475000, China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Breast and Thyroid Surgery, Huaihe Hospital of Henan University, Kaifeng, Henan, 475000, China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Breast and Thyroid Surgery, Huaihe Hospital of Henan University, Kaifeng, Henan, 475000, China.
authors.forename: Shunchang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Breast and Thyroid Surgery, Huaihe Hospital of Henan University, Kaifeng, Henan, 475000, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Breast and Thyroid Surgery, Huaihe Hospital of Henan University, Kaifeng, Henan, 475000, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Radiotherapy, Huaihe Hospital of Henan University, Kaifeng, Henan, 475000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology / Q000528:radiation effects; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000528:radiation effects; D020935:MAP Kinase Signaling System / Q000502:physiology* / Q000528:radiation effects; D011836:Radiation Tolerance / Q000502:physiology*; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000528:radiation effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000096:biosynthesis* / Q000528:radiation effects
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C098669:TOB1 protein, human; D025521:Tumor Suppressor Proteins; D048051:p38 Mitogen-Activated Protein Kinases
keywords: MAPK pathway; Tob1; breast cancer; radiosensitivity; radiotherapy