title: Whole Genome Amplification by Isothermal Multiple Strand Displacement Using Phi29 DNA Polymerase.
abstract: The here described method of isothermal whole genome amplification (iWGA) uses a Phi29 DNA polymerase-based kit (Illustra GenomiPhi V2 DNA Amplification Kit) that amplifies minute quantities of DNA by multiple strand displacement upon random hexamer primer binding. Starting from genomic DNA or single cells this amplification yields up to 5 μg of iWGA product with fragment lengths of 10 kb and longer. As this amplification lacks the need of fragmenting DNA, its products are well suited for many downstream applications (e.g. sequencing and DNA profiling). On the contrary, degraded DNA samples are not supported by the nature of the amplification and are not well suited.
authors.affiliation: Research Unit for Single Cell Analysis, Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Harrachgasse 21, Graz, 8010, Austria. thomas.kroneis@medunigraz.at.|Sahlgrenska Cancer Center, University of Gothenburg, Gothenburg, Sweden. thomas.kroneis@medunigraz.at.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kroneis
authors.affiliation: Research Unit for Single Cell Analysis, Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Harrachgasse 21, Graz, 8010, Austria.
authors.forename: Amin
authors.initials: A
authors.lastname: El-Heliebi
mesh_terms: D001435:Bacteriophages / Q000201:enzymology*; D004259:DNA-Directed DNA Polymerase; D016678:Genome; D023281:Genomics / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques; D011933:Reagent Kits, Diagnostic; D059010:Single-Cell Analysis / Q000379:methods
chemical_list: D011933:Reagent Kits, Diagnostic; D004259:DNA-Directed DNA Polymerase
keywords: Isothermal whole genome amplification; Multiple strand displacement; Phi29 DNA polymerase; Single-cell analysis