title: The influence of three endodontic sealers on bone healing: an experimental study.
abstract: BACKGROUND
  The aim of this experimental study is to assess the bone healing phenomenon produced in the presence of 3 dental materials used for the root canal obturation.
  
  
  METHODS
  The biocompatibility of 3 endodontic sealers (a self-curing epoxy resin - AH Plus, a dual cure urethane dimethacrylate resin - RealSeal and a new dual cure endodontic hydroxyapatite based filling material) was investigated after intra-osseous implantation of the materials in rats' calvaria. Tissue reaction was studied at 2, 4, 6, 8, 10 and 12 weeks after implantation using calibrated image retrieval by Olympus. We took into consideration the presence of inflammatory cells (polymorphonuclear leukocytes, macrophages, plasma cells, lymphocytes and giant cells) and classified the aspects of the histological samples according to the following scale: 0 - no inflammation, 1 - mild, isolated inflammation, 2 - moderate, localised inflammatory reaction, 3 - severe, diffuse and intense inflammatory reaction.
  
  
  RESULTS
  The inflammatory reaction was present at the 6 intervals for all the tested materials, but a decrease of the inflammatory infiltrate, statistically significant, until extinction for all tested materials was observed at the end of the experimental period. The reaction of bone tissue recovery was most intense in the case of the control area. Lower intensity osteogenesis phenomenon was observed in case of all 3 tested sealers at the end of the experimental period.
  
  
  CONCLUSIONS
  Biocompatibility and bone healing induction of the new hydroxyapatite based endodontic filling material is comparable to other commercial materials, AH Plus and RealSeal.
authors.affiliation: Department of Odontology, "Iuliu Hațieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania. maria.chisnoiu@umfcluj.ro.
authors.forename: R
authors.initials: R
authors.lastname: Chisnoiu
authors.forename: M
authors.initials: M
authors.lastname: Moldovan
authors.forename: O
authors.initials: O
authors.lastname: Păstrav
authors.forename: A
authors.initials: A
authors.lastname: Delean
authors.forename: A M
authors.initials: AM
authors.lastname: Chisnoiu
mesh_terms: D000818:Animals; D004853:Epoxy Resins; D007249:Inflammation; D008422:Materials Testing; D019919:Prosthesis Implantation; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials; D014945:Wound Healing
chemical_list: D004853:Epoxy Resins; D012387:Root Canal Filling Materials
keywords: biocompatible materials; bone tissue response; endodontic materials; root canal filling