title: Sub-population analysis of deformability distribution in heterogeneous red blood cell population.
abstract: We present a method for sub-population analysis of deformability distribution using single-cell microchamber array (SiCMA) technology. It is a unique method allowing the correlation of overall cellular characteristics with surface and cytosolic characteristics to define the distribution of individual cellular characteristics in heterogeneous cell populations. As a proof of principle, reticulocytes, the immature sub-population of red blood cells (RBC), were recognized from RBC population by a surface marker and different characteristics on deformability between these populations were characterized. The proposed technology can be used in a variety of applications that would benefit from the ability to measure the distribution of cellular characteristics in complex populations, especially important to define hematologic disorders.
authors.affiliation: Central R&D Institute, Samsung Electro-mechanics Co., Ltd., Suwon, Republic of Korea.
authors.forename: Dong Woo
authors.initials: DW
authors.lastname: Lee
authors.affiliation: Center for Medical Metrology, Korea Research Institute of Standards and Science (KRISS), Daejeon, Republic of Korea. il.doh@kriss.re.kr.
authors.forename: Il
authors.initials: I
authors.lastname: Doh
authors.affiliation: Department of Hematology, Children's Hospital Oakland Research Institute, Oakland, CA, USA.
authors.forename: Frans A
authors.initials: FA
authors.lastname: Kuypers
authors.affiliation: Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Cho
mesh_terms: D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*
keywords: Deformability distribution; Microchamber array; Single cell analysis; Sub-population analysis