title: Fast and simple spectral FLIM for biochemical and medical imaging.
abstract: Spectrally resolved fluorescence lifetime imaging microscopy (λFLIM) has powerful potential for biochemical and medical imaging applications. However, long acquisition times, low spectral resolution and complexity of λFLIM often narrow its use to specialized laboratories. Therefore, we demonstrate here a simple spectral FLIM based on a solid-state detector array providing in-pixel histrogramming and delivering faster acquisition, larger dynamic range, and higher spectral elements than state-of-the-art λFLIM. We successfully apply this novel microscopy system to biochemical and medical imaging demonstrating that solid-state detectors are a key strategic technology to enable complex assays in biomedical laboratories and the clinic.
authors.forename: Marina
authors.initials: M
authors.lastname: Popleteeva
authors.forename: Kalina T
authors.initials: KT
authors.lastname: Haas
authors.forename: David
authors.initials: D
authors.lastname: Stoppa
authors.forename: Lucio
authors.initials: L
authors.lastname: Pancheri
authors.forename: Leonardo
authors.initials: L
authors.lastname: Gasparini
authors.forename: Clemens F
authors.initials: CF
authors.lastname: Kaminski
authors.forename: Liam D
authors.initials: LD
authors.lastname: Cassidy
authors.forename: Ashok R
authors.initials: AR
authors.lastname: Venkitaraman
authors.forename: Alessandro
authors.initials: A
authors.lastname: Esposito
mesh_terms: D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D007909:Lenses; D018613:Microscopy, Confocal / Q000295:instrumentation*; D057054:Molecular Imaging / Q000295:instrumentation*; D061848:Optical Imaging / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation