title: Wire-in-Tube IrOx Architectures: Alternative Label-Free Immunosensor for Amperometric Immunoassay toward α-Fetoprotein.
abstract: A sensitive, label-free immunosensor based on iridium oxide (IrOx, 0≤x≤2) nanofibers, which were synthesized through a simple one-spinneret electrospinning method, was first developed for immunoassay of the cancer biomarker α-fetoprotein (AFP). The specific wire-in-tube nanostructure could be obtained and the composition of IrOx nanofibers also could be controlled through changing the annealing temperature. The unique structure and properties of IrOx nanofibers obtained at 500 °C not only led to increased electrode surface area and accelerated electron transfer kinetics but also could provide a highly stable matrix for the convenient conjugation of biomolecules together with chitosan (CS). The good electrochemical properties of the IrOx-nanofiber-modified immunosensor allowed one to detect AFP over a wide concentration range from 0.05 to 150 ng/mL, with a detection limit of 20 pg/mL. The proposed immunosensor also has been used to determine AFP in human serum with satisfactory results. The present protocol was shown to be quite promising for clinical screening of cancer biomarkers and point-of-care diagnostics applications.
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University , 2699 Qianjin Street, Changchun, 130012, P. R. China.
authors.forename: Qingling
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University , 2699 Qianjin Street, Changchun, 130012, P. R. China.
authors.forename: Dali
authors.initials: D
authors.lastname: Liu
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University , 2699 Qianjin Street, Changchun, 130012, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University , 2699 Qianjin Street, Changchun, 130012, P. R. China.
authors.forename: Ruiqing
authors.initials: R
authors.lastname: Xing
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University , 2699 Qianjin Street, Changchun, 130012, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University , 2699 Qianjin Street, Changchun, 130012, P. R. China.
authors.forename: Kuang
authors.initials: K
authors.lastname: Sheng
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University , 2699 Qianjin Street, Changchun, 130012, P. R. China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Song
mesh_terms: D015374:Biosensing Techniques; D048271:Chitosan / Q000737:chemistry; D055664:Electrochemical Techniques; D006801:Humans; D007118:Immunoassay; D007495:Iridium / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D015203:Reproducibility of Results; D013818:Thermogravimetry; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D000509:alpha-Fetoproteins; C044458:iridium oxide; D007495:Iridium; D048271:Chitosan
keywords: IrOx; electrochemical immunosensor; electrospinning; label-free; wire-in-tube nanostructure; α-fetoprotein