title: Lipase-catalyzed transesterification of soybean oil and phytosterol in supercritical CO2.
abstract: The transesterification of phytosterol and soybean oil was performed using Novozym 435 in supercritical carbon dioxide (SC-CO2). The transesterification reaction was conducted in soybean oil containing 5-25% phytosterol at 55-95 °C and free-water solvent. The effects of temperature, reaction time, phytosterol concentration, lipase dosage and reaction pressure on the conversion rate of transesterification were investigated. The optimal reaction conditions were the reaction temperature (85 °C), reaction time (1 h), phytosterol concentration (5%), reaction pressure (8 Mpa) and lipase dosage (1%). The highest conversion rate of 92% could be achieved under the optimum conditions. Compared with the method of lipase-catalyzed transesterification of phytosterol and soybean oil at normal pressure, the transesterification in SC-CO2 reduced significantly the reaction temperature and reaction time.
authors.affiliation: National Soybean Engineering Technology Research Center, Northeast Agricultural University, 201 Gongbin Road, Xiangfang District, Harbin, 150030, China.|Collaborative Innovation Food Safety and Nutrition Center, Wuxi, 214122, China.
authors.forename: Lizhi
authors.initials: L
authors.lastname: Hu
authors.affiliation: School of Food Science, Northeast Agricultural University, 59 Mucai Road, Xiangfang District, Harbin, 150030, China.
authors.forename: Sun
authors.initials: S
authors.lastname: Llibin
authors.affiliation: School of Food Science, Northeast Agricultural University, 59 Mucai Road, Xiangfang District, Harbin, 150030, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Food Science, Northeast Agricultural University, 59 Mucai Road, Xiangfang District, Harbin, 150030, China.
authors.forename: Liangjun
authors.initials: L
authors.lastname: Qi
authors.affiliation: School of Food Science, Northeast Agricultural University, 59 Mucai Road, Xiangfang District, Harbin, 150030, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Collaborative Innovation Food Safety and Nutrition Center, Wuxi, 214122, China. hlz035d832n@yeah.net.|School of Food Science, Northeast Agricultural University, 59 Mucai Road, Xiangfang District, Harbin, 150030, China. hlz035d832n@yeah.net.
authors.forename: Dianyu
authors.initials: D
authors.lastname: Yu
authors.affiliation: UR Catalyse et Matériaux pour l'Environnement et les Procédés URCMEP (UR11ES85) Faculté des Sciences de Gabès Campus Universitaire Hatem BETTAHAR-Cité Erriadh, 6072, Gabès, Médenine, Tunisia. walid.elfalleh@fst.rnu.tn.
authors.forename: Elfalleh
authors.identifier: http://orcid.org/0000-0002-3493-6451
authors.initials: E
authors.lastname: Walid
authors.affiliation: Collaborative Innovation Food Safety and Nutrition Center, Wuxi, 214122, China. jlzname@163.com.|School of Food Science, Northeast Agricultural University, 59 Mucai Road, Xiangfang District, Harbin, 150030, China. jlzname@163.com.
authors.forename: Lianzhou
authors.initials: L
authors.lastname: Jiang
mesh_terms: D002175:Candida / Q000201:enzymology; D002245:Carbon Dioxide / Q000737:chemistry*; D002384:Catalysis; D004951:Esterification; D008049:Lipase / Q000737:chemistry*; D010840:Phytosterols / Q000032:analysis; D011312:Pressure; D013024:Soybean Oil / Q000737:chemistry*; D013696:Temperature
chemical_list: D010840:Phytosterols; D002245:Carbon Dioxide; D013024:Soybean Oil; D008049:Lipase
keywords: Enzymatic catalysis; Phytosterol esters; Soybean oil; Supercritical CO2; Transesterification