title: Co-expression of Endoxylanase and Endoglucanase in Scheffersomyces stipitis and Its Application in Ethanol Production.
abstract: Scheffersomyces stipitis strain BCC15191 is considered as a biotechnologically valuable yeast for its ability to ferment glucose and xylose, the main sugar components in plant biomass, to ethanol. However, the wild strain lacks of endogenous cellulases and hemicellulases that limited biomass utilization. In order to improve biomass degrading ability of S. stipitis BCC15191, new integrative plasmids harboring constitutive TEF1 promoter and codon-optimized zeocin or hygromycin antibiotic resistance genes were developed. Aspergillus niger endoxylanase and Aspergillus aculeatus endoglucanase activities were demonstrated in transformant cells expressing codon-optimized genes. S. stipitis co-expressing endoxylanase and endoglucanase was able to grow in medium containing xylan and β-glucan as carbon sources and directly produced ethanol with yields of 2.7 g/L. It could also use pretreated corncob as a carbon source for ethanol production. These results suggested that recombinant S. stipilis is possible for consolidated bioprocessing of biomass.
authors.affiliation: Microbial Cell Factory Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand.
authors.forename: Aekkachai
authors.initials: A
authors.lastname: Puseenam
authors.affiliation: Microbial Cell Factory Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand.
authors.forename: Sutipa
authors.initials: S
authors.lastname: Tanapongpipat
authors.affiliation: Microbial Cell Factory Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand. niran.roo@biotec.or.th.
authors.forename: Niran
authors.initials: N
authors.lastname: Roongsawang
mesh_terms: D001230:Aspergillus / Q000201:enzymology; D018533:Biomass; D002480:Cellulase / Q000235:genetics*; D004352:Drug Resistance, Microbial; D000431:Ethanol / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics*; D007218:Industrial Microbiology; D004718:Saccharomycetales / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic
chemical_list: D000431:Ethanol; D006026:Glycoside Hydrolases; C023305:hemicellulase; D002480:Cellulase
keywords: Consolidated bioprocessing; Endoglucanase; Endoxylanase; Scheffersomyces stipitis