title: Effect of magnesium ion on crt gene expression in improving carotenoid yield of Rhodobacter sphaeroides.
abstract: This study aimed at increasing carotenoid yield of Rhodobacter sphaeroides in wastewater treatment by adding magnesium ion (Mg(2+)). Results showed that Mg(2+) could improve R. sphaeroides biomass and carotenoid yield effectively. The highest carotenoid yield of 4.83 ± 0.14 mg/g biomass and biomass production of 3900 ± 180 mg/L were achieved at optimal Mg(2+) concentration of 15 mmol/L. Mechanism analysis revealed that Mg(2+) could promote carotenoid production by regulating the expressions of crt genes. Up-regulation of crtBDA genes improved carotenoid biosynthesis of R. sphaeroides.
authors.affiliation: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Huanghe Road 73, Harbin, 150090, China. liushuli117288@sina.com.
authors.forename: Shuli
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Huanghe Road 73, Harbin, 150090, China. xkli312@163.com.
authors.forename: Xiangkun
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Huanghe Road 73, Harbin, 150090, China. zgm@ruc.edu.cn.|School of Environment and Resource, Renmin University of China, Beijing, 100872, China. zgm@ruc.edu.cn.
authors.forename: Guangming
authors.initials: G
authors.lastname: Zhang
authors.affiliation: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Huanghe Road 73, Harbin, 150090, China. 12b927011@hit.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D018533:Biomass; D002338:Carotenoids / Q000096:biosynthesis / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D008274:Magnesium / Q000494:pharmacology*; D012242:Rhodobacter sphaeroides / Q000187:drug effects* / Q000235:genetics*; D062065:Wastewater / Q000382:microbiology
chemical_list: D002338:Carotenoids; D008274:Magnesium; D062065:Wastewater
keywords: Carotenoid; Gene expression; Magnesium ion; Rhodobacter sphaeroides; Up-regulation