title: Bioconcentration, metabolism and alterations of thyroid hormones of Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) in Zebrafish.
abstract: Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) is an organophosphate widely used as a flame retardant, and has frequently been detected in the environment and biota. The present study investigates the bioconcentration and metabolism of TDCPP in fish. Zebrafish embryos (from 2h post-fertilization) were exposed to TDCPP (0, 4, 20 and 100μg/L) for six months. Exposure to TDCPP significantly induced phase I metabolic enzymes 7-ethoxyresorufin O-deethylase (EROD) and 7-methoxyresorufin O-demethylase (MROD) in fish. The mRNA expression of genes related to Phase I and II metabolic enzymes, such as cyp1a1, cyp1b1, cyp1c1 and ugt1ab were also significantly upregulated. Exposure to TDCPP significantly reduced plasma thyroxine (T4) and 3,5,3'-triiodothyronine (T3) levels in females. Chemicals analysis indicated significant levels of TDCPP and its metabolite, bis (1,3-dichloro-2-propyl) phosphate (BDCPP), in the liver. The present study reveals that exposure to low concentrations of TDCPP can cause bioconcentration in fish, and TDCPP can be readily metabolized in liver.
authors.affiliation: Puai Hospital, Huazhong University of Science and Technology, Wuhan 430033, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, China. Electronic address: wqiangwei@ihb.ac.cn.
authors.forename: Qiangwei
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Qipeng
authors.initials: Q
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Yongyong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Bingsheng
authors.initials: B
authors.lastname: Zhou
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000633:toxicity; D013963:Thyroid Hormones / Q000097:blood*; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D009943:Organophosphorus Compounds; D013963:Thyroid Hormones; D029961:Zebrafish Proteins; D014284:Triiodothyronine; D003577:Cytochrome P-450 Enzyme System; C016805:tris(1,3-dichloro-2-propyl)phosphate; D013974:Thyroxine
keywords: Bioconcentration and metabolism; TDCPP; Thyroid hormones; Zebrafish