title: Fast determination of 24 steroid hormones in river water using magnetic dispersive solid phase extraction followed by liquid chromatography-tandem mass spectrometry.
abstract: The easiness-to-handle of the magnetic dispersive solid phase extraction (Mag-dSPE) procedure was developed for preconcentration of 24 steroid hormones in river water. Ethylenediamine-functionalized magnetic carbon nanotubes (EDA@Mag-CNTs) were synthesised by a simple one-pot reaction and were used as sorbent in Mag-dSPE procedure. The properties of the EDA@Mag-CNTs were characterized by transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). An ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS) method for determination of 24 steroid hormones in river water at nanograms per liter had been developed with pretreatment of the samples by Mag-dSPE. The obtained results demonstrated the higher extraction capacity of EDA@Mag-CNT Mag-dSPE with recoveries between 82.1 and 113%. The limits of quantification (LOQs) for the steroid hormone were between 0.020 and 1.00 ng/L. The developed method had been successfully applied to 60 river water samples, and it was confirmed that EDA@Mag-CNT Mag-dSPE was a highly effective extraction method for the steroid hormone analyses.
authors.affiliation: Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, Zhejiang, China.|Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, Zhejiang, China.
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Zhao
authors.affiliation: Quzhou Center for Disease Control and Prevention, Quzhou, 324000, Zhejiang, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, 315211, Zhejiang, China.
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: Zhan
authors.affiliation: Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, Zhejiang, China.|Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, Zhejiang, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Chen
authors.affiliation: Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, Zhejiang, China.|Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, Zhejiang, China.
authors.forename: Sheng-Dong
authors.initials: SD
authors.lastname: Pan
authors.affiliation: Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, Zhejiang, China. jmcjc@163.com.|Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, Zhejiang, China. jmcjc@163.com.
authors.forename: Mi-Cong
authors.initials: MC
authors.lastname: Jin
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D005029:Ethylenediamines; D012739:Gonadal Steroid Hormones / Q000032:analysis*; D037742:Nanotubes, Carbon / Q000737:chemistry; D045483:Rivers / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D013256:Steroids / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005029:Ethylenediamines; D012739:Gonadal Steroid Hormones; D037742:Nanotubes, Carbon; D013256:Steroids; C031234:ethylenediamine
keywords: Ethylenediamine-functionalized magnetic carbon nanotubes; Magnetic dispersive solid phase extraction; River water; Steroid hormone; Ultra-fast liquid chromatography–tandem quadrupole mass spectrometry