title: IL-4-Producing Dendritic Cells Induced during Schistosoma japonica Infection Promote Th2 Cells via IL-4-Dependent Pathway.
abstract: Although dendritic cells (DCs) have been widely demonstrated to play essential roles in initiation of Th2 responses in helminth infections and allergic reactions, the mechanisms remain uncertain largely because DCs do not produce IL-4. In present investigation, we have uncovered a novel subset of DCs from mice infected with Th2-provoking pathogens Schistosoma japonica, which independently promoted Th2 cells via IL-4-dependent pathway. These DCs contained similar levels of IL-4 mRNA and higher levels of IL-12p40 mRNA comparing to basophils, correlating to their Th2-promoting and Th1-promoting dual polarization capacities. Characterized by expression of FcεRI(+), these DCs were induced independent of T cells. Further investigations revealed that Th2-promoting FcεRI(+) DCs were monocyte-derived inflammatory DCs, which were sufficient to induce Th2 cells in vivo. Egg Ags together with GM-CSF or IL-3 alone were able to stimulate the generation of Th2-promoting FcεRI(+) DCs from bone marrow cells in vitro. To our knowledge, our data for the first time demonstrate that IL-4-producing DCs are induced under some Th2-provoking situations, and they should play important roles in initiation of Th2 response.
authors.affiliation: Department of Immunology and Pathogen Biology, Tongji University School of Medicine, Shanghai 200092, China.
authors.forename: Yi-Lei
authors.initials: YL
authors.lastname: Ma
authors.affiliation: Department of Immunology and Pathogen Biology, Tongji University School of Medicine, Shanghai 200092, China.
authors.forename: Feng-Juan
authors.initials: FJ
authors.lastname: Huang
authors.affiliation: Department of Immunology and Pathogen Biology, Tongji University School of Medicine, Shanghai 200092, China.
authors.forename: Li
authors.initials: L
authors.lastname: Cong
authors.affiliation: Department of Immunology and Pathogen Biology, Tongji University School of Medicine, Shanghai 200092, China.
authors.forename: Wen-Ci
authors.initials: WC
authors.lastname: Gong
authors.affiliation: Department of Immunology and Pathogen Biology, Tongji University School of Medicine, Shanghai 200092, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Bai
authors.affiliation: Department of Immunology and Pathogen Biology, Tongji University School of Medicine, Shanghai 200092, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Immunology and Pathogen Biology, Tongji University School of Medicine, Shanghai 200092, China.
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Chen
authors.affiliation: Department of Immunology and Pathogen Biology, Tongji University School of Medicine, Shanghai 200092, China.
authors.forename: Nian-Yu
authors.initials: NY
authors.lastname: Zhou
authors.affiliation: Department of Immunology and Pathogen Biology, Tongji University School of Medicine, Shanghai 200092, China.
authors.forename: Xiao-Lu
authors.initials: XL
authors.lastname: Jiang
authors.affiliation: Department of Immunology and Pathogen Biology, Tongji University School of Medicine, Shanghai 200092, China yingmsn@163.com xc9718@hotmail.com.
authors.forename: Lan
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of Immunology and Pathogen Biology, Tongji University School of Medicine, Shanghai 200092, China yingmsn@163.com xc9718@hotmail.com.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Chen
mesh_terms: D000818:Animals; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D053762:Interleukin-12 Subunit p40 / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D017455:Receptors, IgE / Q000235:genetics / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology* / Q000473:pathology
chemical_list: D053762:Interleukin-12 Subunit p40; D017455:Receptors, IgE; D015847:Interleukin-4