title: Faster and cleaner real-time pure shift NMR experiments.
abstract: Real-time pure shift experiments provide highly resolved proton NMR spectra which do not require any special processing. Although being more sensitive than their pseudo 2D counterparts, their signal intensities per unit time are still far below regular NMR spectra. In addition, scalar coupling evolution during the individual data chunks produces decoupling sidebands. Here we show that faster and cleaner real-time pure shift spectra can be obtained through the implementation of two parameter alterations. Variation of the FID chunk lengths between individual transients significantly suppresses decoupling sidebands for any kind of real-time pure shift spectra and thus allows for example the analysis of minor components in compound mixtures. Shifting the excitation frequency between individual scans of real-time slice-selective pure shift spectra increases their sensitivity obtainable in unit time by allowing faster repetitions of acquisitions.
authors.affiliation: Institute of Chemistry/Organic and Bioorganic Chemistry, University of Graz, Austria. Electronic address: johannes.mauhart@edu.uni-graz.at.
authors.forename: Johannes
authors.initials: J
authors.lastname: Mauhart
authors.affiliation: Institute of Chemistry/Organic and Bioorganic Chemistry, University of Graz, Austria. Electronic address: simon.glanzer@uni-graz.at.
authors.forename: Simon
authors.initials: S
authors.lastname: Glanzer
authors.affiliation: NMR Laboratory of SANOFI, C&BD (Chemistry & Biotechnology Development Frankfurt Chemistry), Industriepark Hoechst, Building G849, D-65926 Frankfurt/Main, Germany. Electronic address: Peyman.Sakhaii@sanofi.com.
authors.forename: Peyman
authors.initials: P
authors.lastname: Sakhaii
authors.affiliation: Bruker BioSpin GmbH, 76287 Rheinstetten, Germany. Electronic address: wolfgang.bermel@bruker-biospin.de.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Bermel
authors.affiliation: Institute of Chemistry/Organic and Bioorganic Chemistry, University of Graz, Austria. Electronic address: klaus.zangger@uni-graz.at.
authors.forename: Klaus
authors.initials: K
authors.lastname: Zangger
keywords: BIRD; FID data chunking; Homonuclear broadband decoupling; Pure shift NMR; Slice-selective (Zangger–Sterk) decoupling