title: Pressure Modulation of the Enzymatic Activity of Phospholipase A2, A Putative Membrane-Associated Pressure Sensor.
abstract: Phospholipases A2 (PLA2) catalyze the hydrolysis reaction of sn-2 fatty acids of membrane phospholipids and are also involved in receptor signaling and transcriptional pathways. Here, we used pressure modulation of the PLA2 activity and of the membrane's physical-chemical properties to reveal new mechanistic information about the membrane association and subsequent enzymatic reaction of PLA2. Although the effect of high hydrostatic pressure (HHP) on aqueous soluble and integral membrane proteins has been investigated to some extent, its effect on enzymatic reactions operating at the water/lipid interface has not been explored, yet. This study focuses on the effect of HHP on the structure, membrane binding and enzymatic activity of membrane-associated bee venom PLA2, covering a pressure range up to 2 kbar. To this end, high-pressure Fourier-transform infrared and high-pressure stopped-flow fluorescence spectroscopies were applied. The results show that PLA2 binding to model biomembranes is not significantly affected by pressure and occurs in at least two kinetically distinct steps. Followed by fast initial membrane association, structural reorganization of α-helical segments of PLA2 takes place at the lipid water interface. FRET-based activity measurements reveal that pressure has a marked inhibitory effect on the lipid hydrolysis rate, which decreases by 75% upon compression up to 2 kbar. Lipid hydrolysis under extreme environmental conditions, such as those encountered in the deep sea where pressures up to the kbar-level are encountered, is hence markedly affected by HHP, rendering PLA2, next to being a primary osmosensor, a good candidate for a sensitive pressure sensor in vivo.
authors.affiliation: Department of Chemistry and Chemical Biology, Biophysical Chemistry, TU Dortmund University , Otto-Hahn-Str. 6, D-44221 Dortmund, Germany.
authors.forename: Saba
authors.initials: S
authors.lastname: Suladze
authors.affiliation: Department of Chemistry and Chemical Biology, Biophysical Chemistry, TU Dortmund University , Otto-Hahn-Str. 6, D-44221 Dortmund, Germany.
authors.forename: Suleyman
authors.initials: S
authors.lastname: Cinar
authors.affiliation: Department of Chemistry and Chemical Biology, Biophysical Chemistry, TU Dortmund University , Otto-Hahn-Str. 6, D-44221 Dortmund, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Sperlich
authors.affiliation: Department of Chemistry and Chemical Biology, Biophysical Chemistry, TU Dortmund University , Otto-Hahn-Str. 6, D-44221 Dortmund, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Winter
mesh_terms: D015374:Biosensing Techniques; D002462:Cell Membrane / Q000201:enzymology*; D008958:Models, Molecular; D054467:Phospholipases A2 / Q000737:chemistry / Q000378:metabolism*; D011312:Pressure; D011485:Protein Binding; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D054467:Phospholipases A2