title: Nanocomposite of Au Nanoparticles/Helical Carbon Nanofibers and Application in Hydrogen Peroxide Biosensor.
abstract: A combined sol-gel/hydrogen reduction method has been developed for the mass production of helical carbon nanofibers (HCNFs) by the pyrolysis of acetylene at 425 degrees C in the presence of NiO nanoparticles. The synthesized HCNFs were characterized with scanning electron microscopy (SEM), X-ray diffraction (XRD) and high resolution transmission electron microscopy (HRTEM). The helical-structured carbon nanofibers have a large specific surface area and excellent biocompatibility. A novel enzymatic hydrogen peroxide sensor was then successfully fabricated based on the nanocomposites containing HCNFs and gold nanoparticles (AuNPs). The results indicated that the Au/HCNFs nanocomposites exhibited excellent electrocatalytic activity to the reduction of H2O2, offering a wide linear range from 1.0 μM to 3157 μM with a detection limit as low as 0.46 μM. The apparent Michaelis-Menten constant of the biosensor was 0.61 mM. The as-fabricated biosensor showed a rapid and sensitive amperometric response to hydrogen peroxide with acceptable preparation reproducibility and excellent stability. Because of their low cost and high stability, these novel HCNFs represent seem to be a kind of promising biomaterial and may find wide new applications in scopes such as biocatalysis, immunoassay, environmental monitoring and so on.
authors.forename: Mumu
authors.initials: M
authors.lastname: Zhai
authors.forename: Rongjing
authors.initials: R
authors.lastname: Cui
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
authors.forename: Genhua
authors.initials: G
authors.lastname: Zhang
authors.forename: Wang
authors.initials: W
authors.lastname: Lin
authors.forename: Lingjun
authors.initials: L
authors.lastname: Yu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D002244:Carbon; D006046:Gold; D006861:Hydrogen Peroxide