title: SN-38 active loading in poly(lactic-co-glycolic acid) nanoparticles and assessment of their anticancer properties on COLO-205 human colon adenocarcinoma cells.
abstract: SN-38 is a highly effective drug against many cancers. The development of an optimal delivery system for SN-38 is extremely challenging due to its low solubility and labile lactone ring. Herein, SN-38 encapsulated in poly(D,L-lactide-co-glycolide) nanoparticles (NPs) is introduced to enhance its solubility, stability and cellular uptake. SN-38-loaded NPs prepared by spontaneous emulsification solvent diffusion (SESD) method had an average diameter of 310 nm, a zeta potential of -9.69 mV and a loading efficiency of 71%. They were able to protect the active lactone ring of SN-38 against inactivation under physiological condition. A colorectal adenocarcinoma cell line (COLO-205) was used to assess the NPs effects on cytotoxicity and cellular uptake. Result showed a significant decreased cell proliferation and cell apoptosis. These results suggest that these SN-38-loaded NPs can be an effective delivery system for the treatment of colon cancer and potentially for other types of cancers.
authors.affiliation: a Department of Biomedical Engineering , Faculty of Medicine, McGill University , Montreal , Canada .
authors.forename: Sherief
authors.initials: S
authors.lastname: Essa
authors.affiliation: a Department of Biomedical Engineering , Faculty of Medicine, McGill University , Montreal , Canada .
authors.forename: Jamal
authors.initials: J
authors.lastname: Daoud
authors.affiliation: b Department of Chemistry , University of Montreal , Montreal , Canada .
authors.forename: Michel
authors.initials: M
authors.lastname: Lafleur
authors.affiliation: c Department of Computer Engineering , Ecole Polytechnique of Montreal , Montreal , Canada , and.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Martel
authors.affiliation: a Department of Biomedical Engineering , Faculty of Medicine, McGill University , Montreal , Canada .|d Faculty of Dentistry , sMcGill University , Montreal , Canada.
authors.forename: Maryam
authors.initials: M
authors.lastname: Tabrizian
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D000077146:Irinotecan; D019344:Lactic Acid / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D000077146:Irinotecan; D002166:Camptothecin
keywords: Apoptosis; PLGA nanoparticles; SN-38 anticancer drug; SN-38 delivery system; SN-38 solubility and stability; cellular uptake; colorectal adenocarcinomacancer cell; spontaneous emulsification solvent diffusion