title: Prevalence of Providencia Strains among Patients with Diarrhea and in Retail Meats in Thailand.
abstract: In the present study, we examined the prevalence of Providencia spp. in retail meats and in stool samples from diarrheal patients in Thailand. Providencia-specific (Psp)-PCR amplification was positive for 58% (15/26) of the chicken, 64% (16/25) of the pork, and 68% (17/25) of the beef samples. Subsequently, Providencia strains were isolated from 65% (17/26) of the chicken, 68% (17/25) of the pork, and 72% (18/25) of the beef samples. Although P. alcalifaciens (40-58%) was the most prevalent Providencia strain, P. rettgeri, P. stuartii, and P. rustigianii were also isolated. Of the patient stool samples tested, 7.5% (16/214) had Psp-PCR products, of which 4 were P. alcalifaciens (1.9%), 4 were P. rettgeri (1.9%), and 1 was P. stuartii (0.5%). In conclusion, retail meats were substantially contaminated with Providencia, especially P. alcalifaciens. Therefore, retail meats are possibly the source of Providencia infections in humans.
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Shima
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hinenoya
authors.forename: Worada
authors.initials: W
authors.lastname: Samosornsuk
authors.forename: Seksun
authors.initials: S
authors.lastname: Samosornsuk
authors.forename: Narissara
authors.initials: N
authors.lastname: Mungkornkaew
authors.forename: Shinji
authors.initials: S
authors.lastname: Yamasaki
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D004269:DNA, Bacterial / Q000235:genetics*; D003967:Diarrhea / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D004756:Enterobacteriaceae Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission; D005243:Feces / Q000382:microbiology; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011532:Providencia / Q000235:genetics* / Q000302:isolation & purification; D013552:Swine; D013785:Thailand / Q000453:epidemiology
chemical_list: D004269:DNA, Bacterial