title: Effect of 2-deoxy-D-glucose induced metabolic stress on testicular steroidogenesis and antioxidant status in golden hamster, Mesocricetus auratus: Role of photoperiod.
abstract: The driving force underlying the evolution of reproductive seasonality is the marked seasonal fluctuation in energy availability. It is well established that photoperiod regulates testicular steroidogenesis through modulation of circulatory and local melatonin levels and MT1R expression in golden hamster. However, photoperiodic variation in energy availability and its impact on testicular steroidogenesis and antioxidant status has never been investigated for any seasonal breeder. Therefore, the aim of the present study is to elucidate the role of photoperiod in modulation of metabolic stress induced variation in steroidogenesis and antioxidant status in testes. We experimentally reduced energy availability via administration of 2-deoxy-d-glucose (2-DG), a synthetic glucose analog, in critical- (CP), long- (LD) and short-day (SD) exposed golden hamsters, Mesocricetus auratus and examined testicular steroidogenesis and antioxidant status. Administration of 2-DG decreased testicular steroidogenesis and antioxidant enzyme activity in CP and LD experienced hamsters as compared to CP- and LD-control groups. Moreover, a decrease in the plasma- and local-melatonin level and MT1R expression in testes with a simultaneous increase in plasma corticosterone level was noted following 2-DG administration in CP and LD exposed hamsters. In contrast, no significant effect of 2-DG administration was observed on the parameters investigated under SD condition. Therefore, it can be suggested that the reduction in energy availability under CP and LD condition suppressed testicular steroidogenesis and increased oxidative damage in testis whereas SD induced melatonin might have buffered the 2-DG induced suppression of testicular steroidogenesis and maintained testicular antioxidant status.
authors.affiliation: Pineal Research Lab., Department of Zoology, Banaras Hindu University, Varanasi 221005, India. Electronic address: arunmukherjee16@gmail.com.
authors.forename: Arun
authors.initials: A
authors.lastname: Mukherjee
authors.affiliation: Pineal Research Lab., Department of Zoology, Banaras Hindu University, Varanasi 221005, India. Electronic address: chaldar2001@yahoo.com.
authors.forename: Chandana
authors.initials: C
authors.lastname: Haldar
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D003345:Corticosterone / Q000097:blood; D006224:Cricetinae; D003847:Deoxyglucose / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008550:Melatonin / Q000378:metabolism; D008647:Mesocricetus; D017440:Photoperiod; D044122:Receptor, Melatonin, MT1 / Q000378:metabolism; D011974:Receptors, LH / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology
chemical_list: D000975:Antioxidants; D044122:Receptor, Melatonin, MT1; D011974:Receptors, LH; D003847:Deoxyglucose; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D008550:Melatonin; D003345:Corticosterone
keywords: 2-deoxy-d-glucose; Antioxidant enzymes; Golden hamster; Melatonin; Photoperiod; Steroidogenesis; Testes