title: Extended access to methamphetamine self-administration up-regulates dopamine transporter levels 72 hours after withdrawal in rats.
abstract: Previous studies have demonstrated that there are persistent changes in dopamine systems following withdrawal from methamphetamine (METH). This study examined changes in striatal dopamine transporter (DAT), tyrosine hydroxylase (TH) and dopamine receptor 2 (D2) 72 h after withdrawal from METH intravenous self- administration (IVSA). Rats were given limited (1h) or extended (6h) access to METH IVSA (0.05 mg/kg/0.1 ml infusion) for 22 days. Controls did not receive METH IVSA. The rats given extended access to IVSA displayed higher METH intake during the first hour of drug access compared to rats given limited access. Extended access to METH also produced a concomitant increase in striatal DAT levels relative to drug-naïve controls. There were no changes in TH or D2 levels across groups. Previous studies have reported a decrease in striatal DAT levels during protracted periods (>7 days) of withdrawal from METH IVSA. This study extends previous work by showing an increase in striatal DAT protein expression during an earlier time point of withdrawal from this drug. These results are an important step toward understanding the dynamic changes in dopamine systems that occur during different time points of withdrawal from METH IVSA.
authors.affiliation: Departments of Biological Sciences, The University of Texas at El Paso, 500 West University Avenue, El Paso, TX 79968, USA.
authors.forename: Christina
authors.initials: C
authors.lastname: D'Arcy
authors.affiliation: Departments of Biological Sciences, The University of Texas at El Paso, 500 West University Avenue, El Paso, TX 79968, USA.
authors.forename: Joe E
authors.initials: JE
authors.lastname: Luevano
authors.affiliation: Departments of Biological Sciences, The University of Texas at El Paso, 500 West University Avenue, El Paso, TX 79968, USA.
authors.forename: Manuel
authors.initials: M
authors.lastname: Miranda-Arango
authors.affiliation: Departments of Psychology, The University of Texas at El Paso, 500 West University Avenue, El Paso, TX 79968, USA.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Pipkin
authors.affiliation: Departments of Psychology, The University of Texas at El Paso, 500 West University Avenue, El Paso, TX 79968, USA.
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Jackson
authors.affiliation: Departments of Psychology, The University of Texas at El Paso, 500 West University Avenue, El Paso, TX 79968, USA.
authors.forename: Eddie
authors.initials: E
authors.lastname: Castañeda
authors.affiliation: Departments of Biological Sciences, The University of Texas at El Paso, 500 West University Avenue, El Paso, TX 79968, USA.
authors.forename: Kristin L
authors.initials: KL
authors.lastname: Gosselink
authors.affiliation: Departments of Psychology, The University of Texas at El Paso, 500 West University Avenue, El Paso, TX 79968, USA. Electronic address: lodell@utep.edu.
authors.forename: Laura E
authors.initials: LE
authors.lastname: O'Dell
mesh_terms: D000818:Animals; D015259:Dopamine Agents / Q000008:administration & dosage / Q000494:pharmacology*; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism*; D008297:Male; D008694:Methamphetamine / Q000008:administration & dosage / Q000494:pharmacology*; D017072:Neostriatum / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017448:Receptors, Dopamine D2 / Q000378:metabolism*; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D015259:Dopamine Agents; D050483:Dopamine Plasma Membrane Transport Proteins; D017448:Receptors, Dopamine D2; D008694:Methamphetamine; D014446:Tyrosine 3-Monooxygenase
keywords: Abstinence; Dopamine transporter; IVSA; Methamphetamine; Striatum; Withdrawal