title: P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design.
abstract: CONCLUSIONS
  The Plant Small RNA Maker Site (P-SAMS) is a web tool for the simple and automated design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) for efficient and specific targeted gene silencing in plants. P-SAMS includes two applications, P-SAMS amiRNA Designer and P-SAMS syn-tasiRNA Designer. The navigation through both applications is wizard-assisted, and the job runtime is relatively short. Both applications output the sequence of designed small RNA(s), and the sequence of the two oligonucleotides required for cloning into 'B/c' compatible vectors.
  
  
  METHODS
  The P-SAMS website is available at http://p-sams.carringtonlab.org.
  
  
  BACKGROUND
  acarbonell@ibmcp.upv.es or nfahlgren@danforthcenter.org.
authors.affiliation: Donald Danforth Plant Science Center, St. Louis, MO 63132, USA.
authors.forename: Noah
authors.initials: N
authors.lastname: Fahlgren
authors.affiliation: Donald Danforth Plant Science Center, St. Louis, MO 63132, USA.
authors.forename: Steven T
authors.initials: ST
authors.lastname: Hill
authors.affiliation: Donald Danforth Plant Science Center, St. Louis, MO 63132, USA.
authors.forename: James C
authors.initials: JC
authors.lastname: Carrington
authors.affiliation: Donald Danforth Plant Science Center, St. Louis, MO 63132, USA.
authors.forename: Alberto
authors.initials: A
authors.lastname: Carbonell
mesh_terms: D019295:Computational Biology; D020407:Internet; D035683:MicroRNAs / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D012984:Software
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant; D034741:RNA, Small Interfering