title: Chemical Compositions and Aroma Evaluation of Volatile Oil from the Industrial Cultivation Medium of Enterococcus faecalis.
abstract: Enterococcus faecalis is one of the major lactic acid bacterium (LAB) species colonizing the intestines of animals and humans. The characteristic odor of the volatile oils obtained from both the liquid medium after incubation (MAI) and liquid medium before incubation (MBI) in the cultivation process of E. faecalis was investigated to determine the utility of the liquid medium. In total, fifty-six and thirty-two compounds were detected in the volatile oils from the MAI (MAI oil) and MBI (MBI oil), respectively. The principle components of MAI oil were 2,5-dimethylpyrazine (19.3%), phenylacetaldehyde (19.3%), and phenylethyl alcohol (9.3%). The aroma extract dilution analysis (AEDA) method was performed using gas chromatography-olfactometry (GC-O). The total number of aroma-active compounds identified in the volatile oil from MBI and MAI was thirteen compounds; in particular, 5-methyl-2-furanmethanol, phenylacetaldehyde, and phenylethyl alcohol were the most primary aroma-active compounds in MAI oil. These results imply that the industrial cultivation medium after incubation of E. faecalis may be utilized as a source of volatile oils.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University (Kindai University).
authors.forename: Toshirou
authors.initials: T
authors.lastname: Ono
authors.forename: Atsushi
authors.initials: A
authors.lastname: Usami
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakaya
authors.forename: Keisuke
authors.initials: K
authors.lastname: Maeba
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Yonejima
authors.forename: Masanori
authors.initials: M
authors.lastname: Toyoda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ikeda
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Miyazawa
mesh_terms: D000079:Acetaldehyde / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D001431:Bacteriological Techniques; D002849:Chromatography, Gas; D003470:Culture Media / Q000737:chemistry*; D013293:Enterococcus faecalis / Q000378:metabolism*; D007201:Indicator Dilution Techniques; D009812:Odorants / Q000032:analysis*; D009822:Oils, Volatile / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D064367:Olfactometry; D010626:Phenylethyl Alcohol / Q000032:analysis / Q000302:isolation & purification; D011719:Pyrazines / Q000032:analysis / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D009822:Oils, Volatile; D011719:Pyrazines; D000079:Acetaldehyde; D010626:Phenylethyl Alcohol; C013192:phenylacetaldehyde; C075524:2,5-dimethylpyrazine