title: Deltamethrin Binding to Triatoma infestans (Hemiptera: Reduviidae) Lipoproteins. Analysis by Solvent Bar Microextraction Coupled to Gas Chromatography.
abstract: The binding of deltamethrin (DLM) to the hemipteran Triatoma infestans (Klug) hemolymph lipoproteins was evaluated in vitro. After DLM incubation with the insect hemolymph, lipoproteins were fractioned by ultracentrifugation. DLM binding was analyzed by a microextractive technique-solvent bar microextraction-a solventless methodology to extract DLM from each lipoprotein fraction. This is a novel use of the technique applied to extract an insecticide from an insect fluid. Capillary gas chromatography with microelectron capture detection was used to detect DLM bound by the T. infestans hemolymph lipoproteins and to identify the preferred DLM carrier. We show that Lp and VHDLp I lipoproteins are mainly responsible for DLM transport in T. infestans, both in DLM-resistant and DLM-susceptible bugs. Our results also indicate that DLM amounts transported are not related to DLM susceptibility.
authors.affiliation: Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP, CONICET, CCT-La Plata, UNLP), Facultad de Ciencias Médicas 1° piso, calles 60 y 120, La Plata 1900, Argentina.
authors.forename: A B
authors.initials: AB
authors.lastname: Dulbecco
authors.affiliation: Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP, CONICET, CCT-La Plata, UNLP), Facultad de Ciencias Médicas 1° piso, calles 60 y 120, La Plata 1900, Argentina.
authors.forename: S J
authors.initials: SJ
authors.lastname: Mijailovsky
authors.affiliation: Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP, CONICET, CCT-La Plata, UNLP), Facultad de Ciencias Médicas 1° piso, calles 60 y 120, La Plata 1900, Argentina.
authors.forename: J R
authors.initials: JR
authors.lastname: Girotti
authors.affiliation: Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP, CONICET, CCT-La Plata, UNLP), Facultad de Ciencias Médicas 1° piso, calles 60 y 120, La Plata 1900, Argentina. mjuarez@isis.unlp.edu.ar.
authors.forename: M P
authors.initials: MP
authors.lastname: Juárez
mesh_terms: D000818:Animals; D006458:Hemolymph / Q000737:chemistry; D019476:Insect Proteins / Q000737:chemistry*; D007306:Insecticides / Q000737:chemistry*; D008074:Lipoproteins / Q000737:chemistry*; D059627:Liquid Phase Microextraction; D008297:Male; D009570:Nitriles / Q000737:chemistry*; D011722:Pyrethrins / Q000737:chemistry*; D014224:Triatoma / Q000737:chemistry*
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D008074:Lipoproteins; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin
keywords: deltamethrin; insect lipoprotein; insecticide transport; pest control