title: Age distribution of porcine sapovirus asymptomatic infection and molecular evidence of genogroups GIII and GIX? circulation in distinct Brazilian pig production systems.
abstract: This study aimed to evaluate the natural infection by SaV in pigs of different categories of production cycle in an important Brazilian pig-producing region. Faecal samples (n = 169) of suckling, post-weaning, finisher and breeder pig categories were analysed. Animals were from five farrow-to-weaning and nine grower-to-finish commercial pig farms. The RT-PCR assay was performed targeting the partial RNA-dependent RNA polymerase (RdRp) gene of porcine SaV genome. The virus was detected in 23.7% (40/169) of faecal samples and in 10/14 (5/5 farrow-to-weaning; 5/9 grower-to-finish) of pig farms evaluated. Porcine SaV was most frequently (p < 0.05) detected in pigs at post-weaning than in grower-to-finish and breeder categories. The phylogenetic analysis revealed that the porcine SaV strains belong to the GIII and GIX? genogroups. This study showed that the porcine SaV GIII genogroup has spread in the pig herds and provides the first evidence of GIX? genogroup circulation in South America.
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Campus Universitário, PO Box 10011, 86057-970, Londrina, Paraná, Brazil.
authors.forename: Cecília Souza
authors.initials: CS
authors.lastname: Valente
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Campus Universitário, PO Box 10011, 86057-970, Londrina, Paraná, Brazil.|Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Campus Universitário, PO Box 10011, 86057-970, Londrina, Paraná, Brazil.
authors.forename: Alice Fernandes
authors.initials: AF
authors.lastname: Alfieri
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Campus Universitário, PO Box 10011, 86057-970, Londrina, Paraná, Brazil.
authors.forename: Aline Fernandes
authors.initials: AF
authors.lastname: Barry
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Campus Universitário, PO Box 10011, 86057-970, Londrina, Paraná, Brazil.|Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Campus Universitário, PO Box 10011, 86057-970, Londrina, Paraná, Brazil.
authors.forename: Raquel Arruda
authors.initials: RA
authors.lastname: Leme
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Campus Universitário, PO Box 10011, 86057-970, Londrina, Paraná, Brazil.|Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Campus Universitário, PO Box 10011, 86057-970, Londrina, Paraná, Brazil.
authors.forename: Elis
authors.initials: E
authors.lastname: Lorenzetti
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Campus Universitário, PO Box 10011, 86057-970, Londrina, Paraná, Brazil. alfieri@uel.br.|Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Campus Universitário, PO Box 10011, 86057-970, Londrina, Paraná, Brazil. alfieri@uel.br.
authors.forename: Amauri Alcindo
authors.initials: AA
authors.lastname: Alfieri
mesh_terms: D000375:Aging; D000822:Animal Husbandry; D000818:Animals; D058345:Asymptomatic Infections; D001938:Brazil / Q000453:epidemiology; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D005243:Feces / Q000821:virology; D005838:Genotype; D010802:Phylogeny; D029341:Sapovirus / Q000235:genetics*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*
keywords: Faeces; Intestinal health; Molecular detection; Porcine enteric calicivirus; RdRp gene; Swine