title: Peripheral blood NK cells expressing HLA-G, IL-10 and TGF-β in healthy donors and breast cancer patients.
abstract: Human natural killer (NK) cells are not only professional cytotoxic cells integrated into effector branch of innate immunity, but they are also regulatory cells, managing different immune processes. Immunoregulatory NK cells, expressing HLA-G and IL-10, have been generated in vitro from human hematopoietic progenitors and found in vivo among decidual NK cells of pregnant women. Human peripheral blood NK cells have been shown to acquire suppressive properties after HLA-G uptake during trogocytosis. Moreover, it has been shown that circulating NK cells contain a trace amount of cells producing TGF-β and IL-10, which exert a suppressive influence upon innate and adaptive immunity. In this study, we report on a minor subset of peripheral blood HLA-G(+) NK cells possessing suppressive activity toward effector functions of NK cells. Further we demonstrate an increased number of circulating HLA-G(+), IL-10(+), and TGF-β(+) NK cells in breast cancer patients which might impair efficiency of anti-tumor immunity.
authors.affiliation: M.A.Aitkhozhin's Institute of Molecular Biology and Biochemistry, Laboratory of Molecular Immunology and Immunobiotechnology, Almaty, Kazakhstan. Electronic address: katyostapchuk@gmail.com.
authors.forename: Yekaterina O
authors.initials: YO
authors.lastname: Ostapchuk
authors.affiliation: Istanbul University, Institute of Experimental Medicine, Immunology Department, Istanbul, Turkey.
authors.forename: Esin Aktas
authors.initials: EA
authors.lastname: Cetin
authors.affiliation: M.A.Aitkhozhin's Institute of Molecular Biology and Biochemistry, Laboratory of Molecular Immunology and Immunobiotechnology, Almaty, Kazakhstan.
authors.forename: Yuliya V
authors.initials: YV
authors.lastname: Perfilyeva
authors.affiliation: Istanbul University, Institute of Experimental Medicine, Immunology Department, Istanbul, Turkey.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Yilmaz
authors.affiliation: M.A.Aitkhozhin's Institute of Molecular Biology and Biochemistry, Laboratory of Genome, Almaty, Kazakhstan.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Skiba
authors.affiliation: M.A.Aitkhozhin's Institute of Molecular Biology and Biochemistry, Laboratory of Genome, Almaty, Kazakhstan.
authors.forename: Alexandr P
authors.initials: AP
authors.lastname: Chirkin
authors.affiliation: Research Institute of Oncology and Radiology, Mammalogy Center, Almaty, Kazakhstan.
authors.forename: Nazgul A
authors.initials: NA
authors.lastname: Omarbaeva
authors.affiliation: Research Institute of Oncology and Radiology, Mammalogy Center, Almaty, Kazakhstan.
authors.forename: Shynar G
authors.initials: SG
authors.lastname: Talaeva
authors.affiliation: M.A.Aitkhozhin's Institute of Molecular Biology and Biochemistry, Laboratory of Molecular Immunology and Immunobiotechnology, Almaty, Kazakhstan.
authors.forename: Nikolai N
authors.initials: NN
authors.lastname: Belyaev
authors.affiliation: Istanbul University, Institute of Experimental Medicine, Immunology Department, Istanbul, Turkey.
authors.forename: Gunnur
authors.initials: G
authors.lastname: Deniz
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000276:immunology*; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D005260:Female; D059951:HLA-G Antigens / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000276:immunology*; D014407:Tumor Cells, Cultured; D055815:Young Adult
chemical_list: D059951:HLA-G Antigens; C508609:IL10 protein, human; D016212:Transforming Growth Factor beta; D016753:Interleukin-10
keywords: Breast cancer; HLA-G; IL-10; NK cells; TGF-β