title: Over-expression of miR-675 in formalin-fixed paraffin-embedded (FFPE) tissues of breast cancer patients.
abstract: BACKGROUND
  Dysregulation of miR-675 has been found in a variety of solid tumors. MiR-675 has been suggested as having both oncogenic and tumor suppression properties in cancer. However, there is no evidence whether miR-675 is involved in breast cancer. The objective of this study was to evaluate the expression status of miR-675 and its clinical relevance in breast cancer patients.
  
  
  METHODS
  The expression level of miR-675 was detected in 100 breast cancer patients and 38 cancer-free controls using real-time quantitative PCR. The clinicopathological characteristics of miR-675 in breast cancer were also investigated. All statistical analyses were performed using SPSS 20.0.
  
  
  RESULTS
  The study showed that miR-675 was significantly up-regulated in breast cancer patients compared with controls (P < 0.01). There was no significant difference in age, lymph nodes stage, ER status and PR status between patients with and without miR-675 over-expression (P > 0.05). The frequency of miR-675 over-expression was higher in the patients of histological grade I-II than in others (50% versus 9%, P = 0.011). The expression level of miR-675 had a high correlation with miR-24/93/98/378 in breast cancer patients.
  
  
  CONCLUSIONS
  Taken together, our study demonstrated that miR-675 in formalin-fixed paraffin-embedded (FFPE) tissues might serve as a good source for biomarker discovery and breast cancer validation.
authors.affiliation: The Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Zhai
authors.affiliation: The Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: The Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Ling-Yu
authors.initials: LY
authors.lastname: Zhou
authors.affiliation: The Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Yin
authors.affiliation: The Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China ; The Affiliated Jintan Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Tang
authors.affiliation: The Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Tin-Juan
authors.initials: TJ
authors.lastname: Zhang
authors.affiliation: The Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Wang
authors.affiliation: The Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Dong-Qin
authors.initials: DQ
authors.lastname: Yang
authors.affiliation: The Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Lin
authors.affiliation: The Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Zhao-Qun
authors.initials: ZQ
authors.lastname: Deng
keywords: Biomarkers; breast cancer; formalin-fixed paraffin-embedded; miR-675