title: No associations between aromatase gene polymorphisms and breast cancer risk in Saudi patients.
abstract: BACKGROUND
  Cytochrome P450 (CYP)19A1 encodes aromatase, the enzyme responsible for the conversion of androgens to estrogens, and may play a role in variation in outcomes among women with breast cancer. The aim of this study was to analyze the genetic association of rs4646 (A > C) and rs700518 (Val > Val) in the CYP19A1 gene with the risk of breast cancer.
  
  
  METHODS
  These two single nucleotide polymorphisms (SNPs) were analyzed in a primary study group of breast cancer patients and healthy control subjects. Genotypes were determined by the TaqMan SNP analysis technique. The study data were analyzed using the chi-square or t-test and logistic regression analysis by Statistical Package for the Social Sciences version 16 software.
  
  
  RESULTS
  rs4646 and rs700518 had no association with susceptibility to breast cancer. There was no significant association for either of these SNPs overall in breast cancer samples when compared with healthy control samples. Our data do not support a relationship between the CYP19A1 rs4646 and rs700518 SNPs and risk of breast cancer. It may be that there are ethnic differences with regard to this relationship.
  
  
  CONCLUSIONS
  This study demonstrated that CYP19A1 rs4646 and rs700518 SNPs may not be involved in the etiology of breast cancer in the Saudi population. Confirmation of our findings in larger populations of other ethnicities could provide evidence for the role of the CYP19A1 gene in breast carcinomas.
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Alanazi
authors.affiliation: Comprehensive Cancer Center at King Fahad Medical City, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Huda A
authors.initials: HA
authors.lastname: Alabdulkarim
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Jilani P
authors.initials: JP
authors.lastname: Shaik
authors.affiliation: Department of Women's Imaging, King Fahad Medical City, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Abdulrahman
authors.initials: A
authors.lastname: Al Naeem
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Elrobh
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Al Amri
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Fatimah Basil
authors.initials: FB
authors.lastname: Al-Mukaynizi
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Abdelhabib
authors.initials: A
authors.lastname: Semlali
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Arjumand
authors.initials: A
authors.lastname: Warsy
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Narasimha Reddy
authors.initials: NR
authors.lastname: Parine
keywords: CYP19A1; breast cancer; genetic polymorphisms; rs4646; rs700518