title: Synthesis of Amphiphilic Hyperbranched AIE-active Fluorescent Organic Nanoparticles and Their Application in Biological Application.
abstract: Aggregation-induced emission (AIE) dyes have recently attracted much attention for biomedical applications for their remarkable AIE properties. However, the hydrophobic nature of AIE dyes made them difficult to be dispersed in physiological solution and problematic for biomedical application directly. Great efforts have been made to overcome this problem, and different strategies for preparation of water dispersible AIE based nanoprobes had been explored previously. However, a facile and effective strategy is still highly desirable and of great importance for the biomedical applications of AIE dye based on nanoprobes. In this work, the fabrication of amphiphilic hyperbranched fluorescent organic nanoparticles with a core-shell structure based on an AIE dye [tetraphenylethene acrylate (TPE-O-E)] and a hyperbranched polyamino compound [polyethylene imine (PEI)] through Michael addition reaction is described for the first time. The AIE dye as well as the final product PEI-TPE-O-E was characterized in detail by a number of techniques. To test their biomedical application potential, the cell viability as well as cell imaging properties of the PEI-TPE-O-E was also examined. The results showed that the PEI-TPE-O-E organic nanoparticles presented high water dispersiblity, ultrabright fluroerescence, low cytotoxicity and excellent biocompatibility, making them promising for biological imaging and gene delivery applications.
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.|Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
authors.forename: Qiulan
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Department of Chemistry and the Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing, 100084, P. R. China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
authors.forename: Dazhuang
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wan
authors.affiliation: Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
authors.forename: Hongye
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China. liangsd@hotmail.com.
authors.forename: Shangdong
authors.initials: S
authors.lastname: Liang
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China. xiaoyongzhang1980@gmail.com.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and the Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing, 100084, P. R. China. weiyen@tsinghua.edu.cn.
authors.forename: Yen
authors.initials: Y
authors.lastname: Wei
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes