title: REDV Peptide Conjugated Nanoparticles/pZNF580 Complexes for Actively Targeting Human Vascular Endothelial Cells.
abstract: Herein, we demonstrate that the REDV peptide modified nanoparticles (NPs) can serve as a kind of active targeting gene carrier to condensate pZNF580 for specific promotion of the proliferation of endothelial cells (ECs). First, we synthesized a series of biodegradable amphiphilic copolymers by ring-opening polymerization reaction and graft modification with REDV peptide. Second, we prepared active targeting NPs via self-assembly of the amphiphilic copolymers using nanoprecipitation technology. After condensation with negatively charged pZNF580, the REDV peptide modified NPs/pZNF580 complexes were formed finally. Due to the binding affinity toward ECs of the specific peptide, these REDV peptide modified NPs/pZNF580 complexes could be recognized and adhered specifically by ECs in the coculture system of ECs and human artery smooth muscle cells (SMCs) in vitro. After expression of ZNF580, as the key protein to promote the proliferation of ECs, the relative ZNF580 protein level increased from 15.7% to 34.8%. The specificity in actively targeting ECs of the REDV peptide conjugated NPs/pZNF580 complexes was still retained in the coculture system. These findings in the present study could facilitate the development of actively targeting gene carriers for the endothelialization of artificial blood vessels.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, China.|Wenzhou Institute of Biomaterials and Engineering , Wenzhou 325011, China.
authors.forename: Changcan
authors.initials: C
authors.lastname: Shi
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force , Tianjin 300162, China.
authors.forename: Wencheng
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, China.|Joint Laboratory for Biomaterials and Regenerative Medicine, Tianjin University-Helmholtz-Zentrum Geesthacht , Tianjin 300072, China.|Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin) , Tianjin 300072, China.|Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University , Tianjin 300072, China.
authors.forename: Yakai
authors.initials: Y
authors.lastname: Feng
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, China.|Joint Laboratory for Biomaterials and Regenerative Medicine, Tianjin University-Helmholtz-Zentrum Geesthacht , Tianjin 300072, China.
authors.forename: Xiangkui
authors.initials: X
authors.lastname: Ren
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology; D010766:Phosphorylation; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D055767:Immobilized Proteins; D010455:Peptides; D011108:Polymers; D014157:Transcription Factors; C550122:ZNF580 protein, human
keywords: REDV peptide; actively targeting; endothelial cells; gene carriers; nanoparticles; proliferation