title: Ability of Antibiotic-Resistant Nonvaccine-Type Pneumococcal Clones to Cause Otitis Media in an Infant Mouse Model of Pneumococcal-Influenza Virus Coinfection.
abstract: The introduction of the 7-valent pneumococcal conjugate vaccine in Portugal resulted in reduced carriage in children by vaccine-type strains and an increased carriage of three major antibiotic-resistant clones, ST2191, ST276, and ST63 expressing capsules 6A, 19A, and 15A, respectively. Pneumococcal otitis media (OM), a frequent infection among preschool age children, is often associated with viral coinfection. To evaluate the ability of these three antibiotic-resistant clones to cause disease, we used an infant mouse model of influenza virus pneumococcal coinfection. The 6A and 19A clonal types induced OM, while 15A induced pneumococcal pneumonia and bloodstream infection, suggesting potential for invasive disease.
authors.affiliation: 1 Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa , Oeiras, Portugal .|2 Laboratory of Microbiology, The Rockefeller University , New York City, New York.
authors.forename: Nelson
authors.initials: N
authors.lastname: Frazão
authors.affiliation: 3 Laboratory of Pediatric Infectious Diseases, Department of Pediatrics, Radboud University Medical Center , Nijmegen, the Netherlands .|4 Laboratory of Medical Immunology, Department of Laboratory Medicine, Radboud University Medical Center , Nijmegen, the Netherlands .
authors.forename: Peter
authors.initials: P
authors.lastname: Hermans
authors.affiliation: 3 Laboratory of Pediatric Infectious Diseases, Department of Pediatrics, Radboud University Medical Center , Nijmegen, the Netherlands .|4 Laboratory of Medical Immunology, Department of Laboratory Medicine, Radboud University Medical Center , Nijmegen, the Netherlands .
authors.forename: Saskia
authors.initials: S
authors.lastname: van Selm
authors.affiliation: 5 Laboratory of Molecular Microbiology of Human Pathogens, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa , Oeiras, Portugal .
authors.forename: Raquel
authors.initials: R
authors.lastname: Sá-Leão
authors.affiliation: 1 Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa , Oeiras, Portugal .|2 Laboratory of Microbiology, The Rockefeller University , New York City, New York.
authors.forename: Hermínia
authors.initials: H
authors.lastname: de Lencastre
authors.affiliation: 2 Laboratory of Microbiology, The Rockefeller University , New York City, New York.
authors.forename: Alexander
authors.initials: A
authors.lastname: Tomasz
authors.affiliation: 3 Laboratory of Pediatric Infectious Diseases, Department of Pediatrics, Radboud University Medical Center , Nijmegen, the Netherlands .|4 Laboratory of Medical Immunology, Department of Laboratory Medicine, Radboud University Medical Center , Nijmegen, the Netherlands .
authors.forename: Dimitri
authors.initials: D
authors.lastname: Diavatopoulos
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002353:Carrier State / Q000276:immunology / Q000382:microbiology / Q000821:virology; D002999:Clone Cells / Q000187:drug effects / Q000276:immunology; D060085:Coinfection / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology* / Q000821:virology*; D004195:Disease Models, Animal; D024881:Drug Resistance, Bacterial / Q000276:immunology; D000069443:Heptavalent Pneumococcal Conjugate Vaccine / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009975:Orthomyxoviridae / Q000472:pathogenicity; D009976:Orthomyxoviridae Infections / Q000821:virology; D010033:Otitis Media / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology* / Q000821:virology*; D011008:Pneumococcal Infections / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology* / Q000821:virology*; D022242:Pneumococcal Vaccines / Q000276:immunology; D011018:Pneumonia, Pneumococcal / Q000188:drug therapy / Q000382:microbiology / Q000821:virology; D011174:Portugal; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000276:immunology / Q000472:pathogenicity*; D018074:Vaccines, Conjugate / Q000276:immunology
chemical_list: D000900:Anti-Bacterial Agents; D000069443:Heptavalent Pneumococcal Conjugate Vaccine; D022242:Pneumococcal Vaccines; D018074:Vaccines, Conjugate