title: Biodiversity inventories in high gear: DNA barcoding facilitates a rapid biotic survey of a temperate nature reserve.
abstract: BACKGROUND
  Comprehensive biotic surveys, or 'all taxon biodiversity inventories' (ATBI), have traditionally been limited in scale or scope due to the complications surrounding specimen sorting and species identification. To circumvent these issues, several ATBI projects have successfully integrated DNA barcoding into their identification procedures and witnessed acceleration in their surveys and subsequent increase in project scope and scale. The Biodiversity Institute of Ontario partnered with the rare Charitable Research Reserve and delegates of the 6th International Barcode of Life Conference to complete its own rapid, barcode-assisted ATBI of an established land trust in Cambridge, Ontario, Canada.
  
  
  CONCLUSIONS
  The existing species inventory for the rare Charitable Research Reserve was rapidly expanded by integrating a DNA barcoding workflow with two surveying strategies - a comprehensive sampling scheme over four months, followed by a one-day bioblitz involving international taxonomic experts. The two surveys resulted in 25,287 and 3,502 specimens barcoded, respectively, as well as 127 human observations. This barcoded material, all vouchered at the Biodiversity Institute of Ontario collection, covers 14 phyla, 29 classes, 117 orders, and 531 families of animals, plants, fungi, and lichens. Overall, the ATBI documented 1,102 new species records for the nature reserve, expanding the existing long-term inventory by 49%. In addition, 2,793 distinct Barcode Index Numbers (BINs) were assigned to genus or higher level taxonomy, and represent additional species that will be added once their taxonomy is resolved. For the 3,502 specimens, the collection, sequence analysis, taxonomic assignment, data release and manuscript submission by 100+ co-authors all occurred in less than one week. This demonstrates the speed at which barcode-assisted inventories can be completed and the utility that barcoding provides in minimizing and guiding valuable taxonomic specialist time. The final product is more than a comprehensive biotic inventory - it is also a rich dataset of fine-scale occurrence and sequence data, all archived and cross-linked in the major biodiversity data repositories. This model of rapid generation and dissemination of essential biodiversity data could be followed to conduct regional assessments of biodiversity status and change, and potentially be employed for evaluating progress towards the Aichi Targets of the Strategic Plan for Biodiversity 2011-2020.
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Angela C
authors.initials: AC
authors.lastname: Telfer
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Monica R
authors.initials: MR
authors.lastname: Young
authors.affiliation: rare Charitable Research Reserve, Cambridge, Canada.
authors.forename: Jenna
authors.initials: J
authors.lastname: Quinn
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Kate
authors.initials: K
authors.lastname: Perez
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Crystal N
authors.initials: CN
authors.lastname: Sobel
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Jayme E
authors.initials: JE
authors.lastname: Sones
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Valerie
authors.initials: V
authors.lastname: Levesque-Beaudin
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Rachael
authors.initials: R
authors.lastname: Derbyshire
authors.affiliation: CNC, Ottawa, Canada.
authors.forename: Jose
authors.initials: J
authors.lastname: Fernandez-Triana
authors.affiliation: Muséum national d'Histoire Naturelle, Paris, France.
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Rougerie
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Abinah
authors.initials: A
authors.lastname: Thevanayagam
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Adrian
authors.initials: A
authors.lastname: Boskovic
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Alex V
authors.initials: AV
authors.lastname: Borisenko
authors.affiliation: University of Waterloo, Waterloo, Canada.
authors.forename: Alex
authors.initials: A
authors.lastname: Cadel
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Allison
authors.initials: A
authors.lastname: Brown
authors.affiliation: Université de Montpellier, Montpellier, France.
authors.forename: Anais
authors.initials: A
authors.lastname: Pages
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Anibal H
authors.initials: AH
authors.lastname: Castillo
authors.affiliation: EcoBio, Université of Rennes, Rennes, France.
authors.forename: Annegret
authors.initials: A
authors.lastname: Nicolai
authors.affiliation: rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.
authors.forename: Barb Mockford
authors.initials: BM
authors.lastname: Glenn Mockford
authors.affiliation: Museo Argentino de Ciencias Naturales "Bernardino Rivadavia" (MACN-CONICET), Buenos Aires, Argentina.
authors.forename: Belén
authors.initials: B
authors.lastname: Bukowski
authors.affiliation: rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.
authors.forename: Bill
authors.initials: B
authors.lastname: Wilson
authors.affiliation: rare Charitable Research Reserve, Cambridge, Canada.
authors.forename: Brock
authors.initials: B
authors.lastname: Trojahn
authors.affiliation: Biodiversity Institute of Ontario Herbarium, Guelph, Canada.
authors.forename: Carole Ann
authors.initials: CA
authors.lastname: Lacroix
authors.affiliation: University of Waikato, Hamilton, New Zealand.
authors.forename: Chris
authors.initials: C
authors.lastname: Brimblecombe
authors.affiliation: University of Western Ontario, London, Canada.
authors.forename: Christoper
authors.initials: C
authors.lastname: Hay
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Christmas
authors.initials: C
authors.lastname: Ho
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Claudia
authors.initials: C
authors.lastname: Steinke
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Connor P
authors.initials: CP
authors.lastname: Warne
authors.affiliation: University of Guelph, Guelph, Canada.
authors.forename: Cristina
authors.initials: C
authors.lastname: Garrido Cortes
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Daniel
authors.initials: D
authors.lastname: Engelking
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Danielle
authors.initials: D
authors.lastname: Wright
authors.affiliation: Museo Argentino de Ciencias Naturales "Bernardino Rivadavia" (MACN-CONICET), Buenos Aires, Argentina.
authors.forename: Dario A
authors.initials: DA
authors.lastname: Lijtmaer
authors.affiliation: rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Gascoigne
authors.affiliation: Universidad Autonoma de Santo Domingo DR, Santo Domingo, Dominican Republic.
authors.forename: David
authors.initials: D
authors.lastname: Hernandez Martich
authors.affiliation: Myotistar, Cambridge, Canada.
authors.forename: Derek
authors.initials: D
authors.lastname: Morningstar
authors.affiliation: SNSB, Zoologische Staatssammlung Muenchen, Munich, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Neumann
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Dirk
authors.initials: D
authors.lastname: Steinke
authors.affiliation: rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.
authors.forename: Donna DeBruin
authors.initials: DD
authors.lastname: Marco DeBruin
authors.affiliation: University of Guelph, Guelph, Canada.
authors.forename: Dylan
authors.initials: D
authors.lastname: Dobias
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Sears
authors.affiliation: University of Guelph, Guelph, Canada.
authors.forename: Ellen
authors.initials: E
authors.lastname: Richard
authors.affiliation: rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.
authors.forename: Emily
authors.initials: E
authors.lastname: Damstra
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Evgeny V
authors.initials: EV
authors.lastname: Zakharov
authors.affiliation: University of Guelph, Guelph, Canada.
authors.forename: Frederic
authors.initials: F
authors.lastname: Laberge
authors.affiliation: University of Waikato, Hamilton, New Zealand.
authors.forename: Gemma E
authors.initials: GE
authors.lastname: Collins
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Gergin A
authors.initials: GA
authors.lastname: Blagoev
authors.affiliation: rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.
authors.forename: Gerrie
authors.initials: G
authors.lastname: Grainge
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Graham
authors.initials: G
authors.lastname: Ansell
authors.affiliation: Grand River Conservation Authority, Guelph, Canada.
authors.forename: Greg
authors.initials: G
authors.lastname: Meredith
authors.affiliation: University of Waikato, Hamilton, New Zealand.
authors.forename: Ian
authors.initials: I
authors.lastname: Hogg
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Jaclyn
authors.initials: J
authors.lastname: McKeown
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Janet
authors.initials: J
authors.lastname: Topan
authors.affiliation: rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.
authors.forename: Jason
authors.initials: J
authors.lastname: Bracey
authors.affiliation: rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.
authors.forename: Jerry
authors.initials: J
authors.lastname: Guenther
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Jesse
authors.initials: J
authors.lastname: Sills-Gilligan
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Joseph
authors.initials: J
authors.lastname: Addesi
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Joshua
authors.initials: J
authors.lastname: Persi
authors.affiliation: The University of Western Australia, Perth, Australia.
authors.forename: Kara K S
authors.initials: KK
authors.lastname: Layton
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Kareina
authors.initials: K
authors.lastname: D'Souza
authors.affiliation: National Biodiversity Centre, Thimphu, Bhutan.
authors.forename: Kencho
authors.initials: K
authors.lastname: Dorji
authors.affiliation: rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.
authors.forename: Kevin
authors.initials: K
authors.lastname: Grundy
authors.affiliation: Ministry of Environment and Tourism in Namibia, Windhoek, Namibia.
authors.forename: Kirsti
authors.initials: K
authors.lastname: Nghidinwa
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Kylee
authors.initials: K
authors.lastname: Ronnenberg
authors.affiliation: University of Oulu, Oulu, Finland.
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Lee
authors.affiliation: The University of Western Ontario, London, Canada.
authors.forename: Linxi
authors.initials: L
authors.lastname: Xie
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Liuqiong
authors.initials: L
authors.lastname: Lu
authors.affiliation: Pensoft, Sofia, Bulgaria.
authors.forename: Lyubomir
authors.initials: L
authors.lastname: Penev
authors.affiliation: Instituto de Investigación de Recursos Biológicos Alexander von Humboldt, Bogotá, Colombia.
authors.forename: Mailyn
authors.initials: M
authors.lastname: Gonzalez
authors.affiliation: Smithsonian National Museum of Natural History, Washington, United States of America.
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Rosati
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Mari
authors.initials: M
authors.lastname: Kekkonen
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Maria
authors.initials: M
authors.lastname: Kuzmina
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Marianne
authors.initials: M
authors.lastname: Iskandar
authors.affiliation: University of Oulu, Oulu, Finland.
authors.forename: Marko
authors.initials: M
authors.lastname: Mutanen
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Maryam
authors.initials: M
authors.lastname: Fatahi
authors.affiliation: University of Oulu, Oulu, Finland.
authors.forename: Mikko
authors.initials: M
authors.lastname: Pentinsaari
authors.affiliation: rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.
authors.forename: Miriam
authors.initials: M
authors.lastname: Bauman
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Nadya
authors.initials: N
authors.lastname: Nikolova
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Ivanova
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Jones
authors.affiliation: The University of Western Ontario, London, Canada.
authors.forename: Nimalka
authors.initials: N
authors.lastname: Weerasuriya
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Norman
authors.initials: N
authors.lastname: Monkhouse
authors.affiliation: Museo Argentino de Ciencias Naturales "Bernardino Rivadavia" (MACN-CONICET), Buenos Aires, Argentina.
authors.forename: Pablo D
authors.initials: PD
authors.lastname: Lavinia
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Paul
authors.initials: P
authors.lastname: Jannetta
authors.affiliation: Museo Argentino de Ciencias Naturales "Bernardino Rivadavia" (MACN-CONICET), Buenos Aires, Argentina.
authors.forename: Priscila E
authors.initials: PE
authors.lastname: Hanisch
authors.affiliation: Biodiversity Institute of Ontario Herbarium, Guelph, Canada.
authors.forename: R Troy
authors.initials: RT
authors.lastname: McMullin
authors.affiliation: Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Rafael
authors.initials: R
authors.lastname: Ojeda Flores
authors.affiliation: ANSES, Laboratoire de la Santé des Végétaux, Montferrier sur Lez, France.
authors.forename: Raphaëlle
authors.initials: R
authors.lastname: Mouttet
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Reid
authors.initials: R
authors.lastname: Vender
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Renee N
authors.initials: RN
authors.lastname: Labbee
authors.affiliation: New Brunswick Museum, Saint John, Canada.
authors.forename: Robert
authors.initials: R
authors.lastname: Forsyth
authors.affiliation: London Homeopathy, London, Canada.
authors.forename: Rob
authors.initials: R
authors.lastname: Lauder
authors.affiliation: rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.
authors.forename: Ross
authors.initials: R
authors.lastname: Dickson
authors.affiliation: rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.
authors.forename: Ruth
authors.initials: R
authors.lastname: Kroft
authors.affiliation: Smithsonian National Museum of Natural History, Washington, United States of America.
authors.forename: Scott E
authors.initials: SE
authors.lastname: Miller
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Shannon
authors.initials: S
authors.lastname: MacDonald
authors.affiliation: Ministry of Forests and Soil Conservation, Kathmandu, Nepal.
authors.forename: Sishir
authors.initials: S
authors.lastname: Panthi
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Pedersen
authors.affiliation: rare Charitable Research Reserve, Cambridge, Canada.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Sobek-Swant
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Suresh
authors.initials: S
authors.lastname: Naik
authors.affiliation: Scientific and Practical Center for Bioresources, National Academy of Sciences of Belarus, Minsk, Belarus.
authors.forename: Tatsiana
authors.initials: T
authors.lastname: Lipinskaya
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Thanushi
authors.initials: T
authors.lastname: Eagalle
authors.affiliation: Université de Montpellier Centre d'Ecologie Fonctionnelle et Evolutive, Montpellier, France.
authors.forename: Thibaud
authors.initials: T
authors.lastname: Decaëns
authors.affiliation: Université de Montpellier, Montpellier, France.
authors.forename: Thibault
authors.initials: T
authors.lastname: Kosuth
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Thomas
authors.initials: T
authors.lastname: Braukmann
authors.affiliation: rare Charitable Research Reserve, Cambridge, Canada.
authors.forename: Tom
authors.initials: T
authors.lastname: Woodcock
authors.affiliation: University of Helsinki, Helsinki, Finland ; Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Tomas
authors.initials: T
authors.lastname: Roslin
authors.affiliation: Grand River Conservation Authority, Cambridge, Canada.
authors.forename: Tony
authors.initials: T
authors.lastname: Zammit
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Victoria
authors.initials: V
authors.lastname: Campbell
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Vlad
authors.initials: V
authors.lastname: Dinca
authors.affiliation: Bulgarian Academy of Sciences, Sofia, Bulgaria.
authors.forename: Vlada
authors.initials: V
authors.lastname: Peneva
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Paul D N
authors.initials: PD
authors.lastname: Hebert
authors.affiliation: Biodiversity Institute of Ontario, Guelph, Canada.
authors.forename: Jeremy R
authors.initials: JR
authors.lastname: deWaard
keywords: Barcode Index Numbers; DNA barcoding; Operational Taxonomic Units; biodiversity assessment; biotic inventory; rare Charitable Research Reserve; species identification