title: Thermoresponsive hydrogel maintains the mouse embryonic stem cell "naïve" pluripotency phenotype.
abstract: A chemically defined thermoresponsive hydrogel, poly(AEtMA-Cl-co-DEAEA) cross-linked with N,N'-methylenebisacrylamide, which allows enzyme-free passaging, was used as a substrate to culture murine embryonic stem cells (mESCs) under defined and undefined conditions. Analysis of 14 stem cell markers showed that the mESCs remained in a "naïve" state of pluripotency with differentiation potential to form endoderm, mesoderm, and ectoderm derived lineages. These results validate the use of a chemically defined hydrogel for standardised and inexpensive mESC culture.
authors.affiliation: School of Chemistry, EaStCHEM, University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh, EH9 3FJ, UK. Mark.Bradley@ed.ac.uk.
authors.forename: Christian
authors.initials: C
authors.lastname: Mangani
authors.forename: Annamaria
authors.initials: A
authors.lastname: Lilienkampf
authors.forename: Marcia
authors.initials: M
authors.lastname: Roy
authors.forename: Paul A
authors.initials: PA
authors.lastname: de Sousa
authors.forename: Mark
authors.initials: M
authors.lastname: Bradley
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000737:chemistry* / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D013696:Temperature
chemical_list: D000178:Acrylamides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C021221:N,N'-methylenebisacrylamide