title: Liquid Crystal Formulation and Optimization of Anti-Microbial Polyherbal Ointment.
abstract: We examined the formulation of liquid crystalline systems (LCS) including 5% TSE extracts and analyzed marker substances of the 5% TSE ointment by HPLC-DAD. The TSE extracts were evaluated for its anti-bacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. We found the extracts showed predominant activity against selected bacterial species. The result of the polarized light microscopy, differential scanning calorimetry (DSC), small-angle X-ray diffraction (SXRD), and rheology analysis indicated the presence of LCS structures with lamellar arrangement. DSC of the TSE formulas showed higher transition peak temperature at 60 °c for the phase. SXRD observation of the LCS formulas showed that the structures of the LCS formulas were in the lamellar liquid crystalline phase. Further, to ensure the quality and purity of the TSE ointment, HPLC analysis was performed by measuring the. content of 2 marker substances. The contents of marker substances in the TSE ointment were calculated as 0.078% (paeoniflorin) and 0.031% (glycyrrhizin), respectively. Taken altogether, our study report successful generation of LCS made of 5% TSE ointment and its antimicrobial activity. Moreover, the quantitation of the two active components enable a proper quality control of the TSE extracts, that is essential for the development of ointment products.
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Choi
authors.forename: Cheong-Weon
authors.initials: CW
authors.lastname: Cho
authors.forename: Jae-Hun
authors.initials: JH
authors.lastname: Kim
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Suhwan
authors.initials: S
authors.lastname: Chang
authors.forename: Young-Beob
authors.initials: YB
authors.lastname: Yu
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000138:chemical synthesis; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry*; D004338:Drug Combinations; D004339:Drug Compounding / Q000379:methods; D015195:Drug Design; D050866:Liquid Crystals / Q000737:chemistry*; D009824:Ointments / Q000008:administration & dosage* / Q000138:chemical synthesis; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D004338:Drug Combinations; D009824:Ointments; D010936:Plant Extracts