title: Physical training improves body weight and energy balance but does not protect against hepatic steatosis in obese mice.
abstract: This study sought to determine the role of physical training (PT) on body weight (BW), energy balance, histological markers of nonalcoholic fatty liver disease (NAFLD) and metabolic gene expression in the liver of ob/ob mice. Adult male ob/ob mice were assigned into groups sedentary (S; n = 8) and trained (T; n = 9). PT consisted in running sessions of 60 min at 60% of maximal speed conducted five days per week for eight weeks. BW of S group was higher from the 4(th) to 8(th) week of PT compared to their own BW at the beginning of the experiment. PT decreased daily food intake and increased resting oxygen consumption and energy expenditure in T group. No difference was observed in respiratory exchange ratio, but the rates of carbohydrate and lipids oxidation, and maximal running capacity were greater in T than S group. Both groups showed liver steatosis but not inflammation. PT increased CPT1a and SREBP1c mRNA expression in T group, but did not change MTP, PPAR-α, PPAR-γ, and NFKB mRNA expression. In conclusion, PT prevented body weight gain in ob/ob mice by inducing negative energy balance and increased physical exercise tolerance. However, PT did not change inflammatory gene expression and failed to prevent liver steatosis possible due to an upregulation in the expression of SREBP1c transcription factor. These findings reveal that PT has positive effect on body weight control but not in the liver steatosis in a leptin deficiency condition.
authors.affiliation: School of Arts, Science and Humanities, University of Sao Paulo Brazil.
authors.forename: Fabiana S
authors.initials: FS
authors.lastname: Evangelista
authors.affiliation: Experimental Pathophysiology Dept, Faculty of Medicine, University of Sao Paulo Brazil.
authors.forename: Cynthia R
authors.initials: CR
authors.lastname: Muller
authors.affiliation: Gastroenterology Dept (LIM 07), Faculty of Medicine, University of Sao Paulo Brazil.
authors.forename: Jose T
authors.initials: JT
authors.lastname: Stefano
authors.affiliation: Gastroenterology Dept (LIM 07), Faculty of Medicine, University of Sao Paulo Brazil.
authors.forename: Mariana M
authors.initials: MM
authors.lastname: Torres
authors.affiliation: Gastroenterology Dept (LIM 07), Faculty of Medicine, University of Sao Paulo Brazil.
authors.forename: Bruna R
authors.initials: BR
authors.lastname: Muntanelli
authors.affiliation: Molecular and Cellular Biology Applied to Health Dept, Luterana University of Brazil (ULBRA) Canoas, RS, Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Simon
authors.affiliation: Division of Gastroenterology, Clinics Hospital of Porto Alegre, Federal University of Rio Grande do Sul Brazil.
authors.forename: Mario R
authors.initials: MR
authors.lastname: Alvares-da-Silva
authors.affiliation: Gastroenterology Dept (LIM 07), Faculty of Medicine, University of Sao Paulo Brazil.
authors.forename: Isabel V
authors.initials: IV
authors.lastname: Pereira
authors.affiliation: Gastroenterology Dept (LIM 07), Faculty of Medicine, University of Sao Paulo Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Cogliati
authors.affiliation: Gastroenterology Dept (LIM 07), Faculty of Medicine, University of Sao Paulo Brazil.
authors.forename: Flair J
authors.initials: FJ
authors.lastname: Carrilho
authors.affiliation: Gastroenterology Dept (LIM 07), Faculty of Medicine, University of Sao Paulo Brazil.
authors.forename: Claudia P
authors.initials: CP
authors.lastname: Oliveira
keywords: Obesity; energy balance; gene expression; leptin-deficient mice; liver steatosis