title: Structure-directed construction of a high-performance version of the enzyme FabG from the photosynthetic microorganism Synechocystis sp. PCC 6803.
abstract: PhaB (acetoacetyl-CoA reductase) catalyzes the reduction of acetoacetyl-CoA to (R)-3-hydroxybutyryl-CoA in polyhydroxybutyrate (PHB) synthesis and FabG (3-ketoacyl-acyl-carrier-protein reductase) catalyzes the β-ketoacyl-ACP to yield (R)-3-hydroxyacyl-ACP in fatty acid biosynthesis. Both of them have been classified into the same group EC 1.1.1. PhaB is limited with substrate specificities, while FabG was considered as a potential PhaB due to broad substrate selectivity despite of low activity. Here, X-ray crystal structures of FabG and PhaB from the photosynthetic microorganism Synechocystis sp. PCC 6803 were resolved. Based on them, a high-performance FabG on acyl-CoA directed by structural evolution was constructed that may serve as a critical enzyme to partition carbon flow from fatty acid synthesis to PHA.
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Xupeng
authors.initials: X
authors.lastname: Cao
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: xuesong@dicp.ac.cn.
authors.forename: Song
authors.initials: S
authors.lastname: Xue
mesh_terms: D064431:3-Oxoacyl-(Acyl-Carrier-Protein) Reductase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000213:Acyl Carrier Protein / Q000737:chemistry / Q000378:metabolism; D000214:Acyl Coenzyme A / Q000737:chemistry / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009154:Mutation; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D046939:Synechocystis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D000213:Acyl Carrier Protein; D000214:Acyl Coenzyme A; D001426:Bacterial Proteins; C010667:acetoacetyl CoA; D000429:Alcohol Oxidoreductases; D064431:3-Oxoacyl-(Acyl-Carrier-Protein) Reductase; C023744:acetoacetyl-CoA reductase
keywords: 3-Ketoacyl-acyl-carrier-protein reductase; Acetoacetyl-CoA reductase; Polyhydroxyalkanoates; Structure; Synechocystis sp. PCC 6803