title: Laser Microdissection of FFPE Tissue Areas and Subsequent Whole Genome Amplification by Ampli1™.
abstract: Laser microdissection (LMD) and whole genome amplification (WGA) are valuable tools to isolate, purify, and genetically analyze cancer cells from tissue sections. In this chapter, we describe a workflow for microdissecting small regions of interest from cancer tissue, i.e. formalin-fixed paraffin-embedded (FFPE) and cryo-conserved specimens, and subsequent whole genome amplification by a deterministic WGA approach (Ampli1™ WGA).
authors.affiliation: Project Group, Personalized Tumor Therapy, Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Josef-Engert-Straße 9, 93053, Regensburg, Germany.
authors.forename: Zbigniew Tadeusz
authors.initials: ZT
authors.lastname: Czyz
authors.affiliation: Department of Visceral, General, and Pediatric Surgery, University Hospital and Medical Faculty of the Heinrich-Heine University Düsseldorf, Moorenstr. 5, 40225, Düsseldorf, Germany.
authors.forename: Nikolas H
authors.initials: NH
authors.lastname: Stoecklein
authors.affiliation: Project Group, Personalized Tumor Therapy, Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Josef-Engert-Straße 9, 93053, Regensburg, Germany. bernhard.michael.polzer@item.fraunhofer.de.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Polzer
mesh_terms: D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D006801:Humans; D061346:Laser Capture Microdissection / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods
keywords: Formalin-fixed paraffin-embedded (FFPE) tissue; Laser microdissection (LMD); Whole genome amplification (WGA)