title: Plastid Proteomic Analysis in Tomato Fruit Development.
abstract: To better understand the mechanism of plastid differentiation from chloroplast to chromoplast, we examined proteome and plastid changes over four distinct developmental stages of 'Micro-Tom' fruit. Additionally, to discover more about the relationship between fruit color and plastid differentiation, we also analyzed and compared 'Micro-Tom' results with those from two other varieties, 'Black' and 'White Beauty'. We confirmed that proteins related to photosynthesis remain through the orange maturity stage of 'Micro-Tom', and also learned that thylakoids no longer exist at this stage. These results suggest that at a minimum there are changes in plastid morphology occurring before all related proteins change. We also compared 'Micro-Tom' fruits with 'Black' and 'White Beauty' using two-dimensional gel electrophoresis. We found a decrease of CHRC (plastid-lipid-associated protein) and HrBP1 (harpin binding protein-1) in the 'Black' and 'White Beauty' varieties. CHRC is involved in carotenoid accumulation and stabilization. HrBP1 in Arabidopsis has a sequence similar to proteins in the PAP/fibrillin family. These proteins have characteristics and functions similar to lipocalin, an example of which is the transport of hydrophobic molecules. We detected spots of TIL (temperature-induced lipocalin) in 2D-PAGE results, however the number of spots and their isoelectric points differed between 'Micro-Tom' and 'Black'/'White Beauty'. Lipocalin has various functions including those related to environmental stress response, apoptosis induction, membrane formation and fixation, regulation of immune response, cell growth, and metabolism adjustment. Lipocalin related proteins such as TIL and HrBP1 could be related to the accumulation of carotenoids, fruit color and the differentiation of chromoplast.
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka city, Shizuoka, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka city, Shizuoka, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Takahashi
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka city, Shizuoka, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Kondo
authors.affiliation: Instrumental Research Support Office, Research Institute of Green Science and Technology, Shizuoka University, Shizuoka city, Shizuoka, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Dohra
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka city, Shizuoka, Japan.
authors.forename: Yumihiko
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka city, Shizuoka, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kiriiwa
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka city, Shizuoka, Japan.
authors.forename: Marina
authors.initials: M
authors.lastname: Hayashi
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka city, Shizuoka, Japan.
authors.forename: Shiori
authors.initials: S
authors.lastname: Kamiya
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka city, Shizuoka, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Kato
authors.affiliation: The Plant Science Education Unit, Nara Institute of Science and Technology, Ikoma city, Nara, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Fujiwara
authors.affiliation: The Plant Science Education Unit, Nara Institute of Science and Technology, Ikoma city, Nara, Japan.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Fukao
authors.affiliation: Faculty of Science, Japan Woman's University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: Faculty of Science, Japan Woman's University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Nagata
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka city, Shizuoka, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Motohashi
mesh_terms: D002338:Carotenoids / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D005419:Flavonoids / Q000378:metabolism; D005638:Fruit / Q000254:growth & development*; D018551:Solanum lycopersicum / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010858:Pigmentation; D010940:Plant Proteins / Q000378:metabolism; D018087:Plastids / Q000378:metabolism*; D040901:Proteomics
chemical_list: D005419:Flavonoids; D010940:Plant Proteins; D002338:Carotenoids