title: Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents.
abstract: Synaptic tagging and capture (STC) and cross-tagging are two important mechanisms at cellular level that explain how synapse-specificity and associativity is achieved in neurons within a specific time frame. These long-term plasticity-related processes are the leading candidate models to study the basis of memory formation and persistence at the cellular level. Both STC and cross-tagging involve two serial processes: (1) setting of the synaptic tag as triggered by a specific pattern of stimulation, and (2) synaptic capture, whereby the synaptic tag interacts with newly synthesized plasticity-related proteins (PRPs). Much of the understanding about the concepts of STC and cross-tagging arises from the studies done in CA1 region of the hippocampus and because of the technical complexity many of the laboratories are still unable to study these processes. Experimental conditions for the preparation of hippocampal slices and the recording of stable late-LTP/LTD are extremely important to study synaptic tagging/cross-tagging. This video article describes the experimental procedures to study long-term plasticity processes such as STC and cross-tagging in the CA1 pyramidal neurons using stable, long-term field-potential recordings from acute hippocampal slices of rats.
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore; Neurobiology/Aging Programme, Life Sciences Institute, National University of Singapore.
authors.forename: Mahesh Shivarama
authors.initials: MS
authors.lastname: Shetty
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore; Neurobiology/Aging Programme, Life Sciences Institute, National University of Singapore.
authors.forename: Mahima
authors.initials: M
authors.lastname: Sharma
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore; Neurobiology/Aging Programme, Life Sciences Institute, National University of Singapore.
authors.forename: Neo Sin
authors.initials: NS
authors.lastname: Hui
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore; Neurobiology/Aging Programme, Life Sciences Institute, National University of Singapore.
authors.forename: Ananya
authors.initials: A
authors.lastname: Dasgupta
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore; Neurobiology/Aging Programme, Life Sciences Institute, National University of Singapore.
authors.forename: Suma
authors.initials: S
authors.lastname: Gopinadhan
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore; Neurobiology/Aging Programme, Life Sciences Institute, National University of Singapore; phssks@nus.edu.sg.
authors.forename: Sreedharan
authors.initials: S
authors.lastname: Sajikumar
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000166:cytology / Q000502:physiology*; D008297:Male; D009473:Neuronal Plasticity / Q000502:physiology; D017966:Pyramidal Cells / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000502:physiology*