title: One-pot synthesized functionalized mesoporous silica as a reversed-phase sorbent for solid-phase extraction of endocrine disrupting compounds in milks.
abstract: A new procedure for the determination of 12 naturally occurring hormones and some related synthetic chemicals in milk, commonly used as growth promoters in cattle, is reported. The method is based on liquid-liquid extraction followed by solid-phase extraction (SPE) using a new one-pot synthesized ordered mesoporous silica (of the SBA-15 type) functionalized with octadecyl groups (denoted as SBA-15-C18-CO) as reversed-phase sorbent. The analytes were eluted with methanol and then submitted to HPLC with diode array detection. Under optimal conditions, the method quantification limit for the analytes ranged from 0.023 to 1.36μg/mL. The sorbent affored the extraction of estrone, 17β-estradiol, estriol, progesterone, hexestrol, diethylstilbestrol, 4-androstene-3,17-dione, ethinylestradiol, 17α-methyltestosterone, nandrolone, prednisolone and testosterone with mean recoveries ranging from 72% to 105% (except for diethylstilbestrol) with RSD<11%. These results were comparable and, in some cases, even better than those obtained with other extraction methods, therefore SBA-15-C18-CO mesoporous silica possess a high potential as a reversed-phase sorbent for SPE of the 12 mentioned endocrine disrupting compounds in milk samples.
authors.affiliation: Departamento de Tecnología Química y Energética, Tecnología Química y Ambiental, Tecnología Mecánica y Química Analítica, E.S.C.E.T, Universidad Rey Juan Carlos, C/ Tulipán s/n, 28933 Móstoles, Madrid, Spain.
authors.forename: Judith
authors.initials: J
authors.lastname: Gañán
authors.affiliation: Departamento de Tecnología Química y Energética, Tecnología Química y Ambiental, Tecnología Mecánica y Química Analítica, E.S.C.E.T, Universidad Rey Juan Carlos, C/ Tulipán s/n, 28933 Móstoles, Madrid, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Morante-Zarcero
authors.affiliation: Departamento de Tecnología Química y Energética, Tecnología Química y Ambiental, Tecnología Mecánica y Química Analítica, E.S.C.E.T, Universidad Rey Juan Carlos, C/ Tulipán s/n, 28933 Móstoles, Madrid, Spain.
authors.forename: Damián
authors.initials: D
authors.lastname: Pérez-Quintanilla
authors.affiliation: Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Marina
authors.affiliation: Departamento de Tecnología Química y Energética, Tecnología Química y Ambiental, Tecnología Mecánica y Química Analítica, E.S.C.E.T, Universidad Rey Juan Carlos, C/ Tulipán s/n, 28933 Móstoles, Madrid, Spain. Electronic address: isabel.sierra@urjc.es.
authors.forename: Isabel
authors.initials: I
authors.lastname: Sierra
mesh_terms: D000735:Androstenedione / Q000737:chemistry; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004054:Diethylstilbestrol; D052244:Endocrine Disruptors / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D059625:Liquid-Liquid Extraction; D008892:Milk / Q000737:chemistry*; D012822:Silicon Dioxide / Q000138:chemical synthesis* / Q000737:chemistry; D052616:Solid Phase Extraction
chemical_list: D052244:Endocrine Disruptors; C509969:SBA-15; D000735:Androstenedione; D004054:Diethylstilbestrol; D012822:Silicon Dioxide
keywords: Endocrine disrupting compounds estrogens; Milk; SBA-15; Solid-phase extraction