title: Enzymolysis kinetics, thermodynamics and model of porcine cerebral protein with single-frequency countercurrent and pulsed ultrasound-assisted processing.
abstract: The present work investigated the enzymolysis kinetics, thermodynamics and model of porcine cerebral protein (PCP) which was pretreated by single-frequency countercurrent and pulsed ultrasound. The kinetic constants for ultrasonic pretreated and traditional enzymolysis have been determined. Results showed that the value of KM in ultrasonic PCP (UPCP) enzymolysis decreased by 9% over that in the traditional enzymolysis. The values of reaction rate constant (k) for UPCP enzymolysis increased by 207%, 121%, 62%, and 45% at 293, 303, 313 and 323 K, respectively. For the thermodynamic parameters, ultrasound decreased activation energy (Ea), change in enthalpy (ΔH) and entropy (ΔS) by 76%, 82% and 31% in PCP, respectively. However, ultrasound had little change in Gibbs free energy (ΔG) value in the temperature range of 293-323 K. Therefore, a general kinetic equation for the enzymolysis model of UPCP by a simple empirical equation was suggested. The experimental values fits with the enzymolysis kinetic model with a low average relative error (4%) confirmed that the kinetic model was accurate to reflect the enzymolysis process. The positive effect of single-frequency countercurrent and pulsed ultrasound in this study and application of the kinetic model may be useful for the release of bioactive peptides from meat processing by-products.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zou
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Ding
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Feng
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of the Environment and Safety, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Huiyu
authors.initials: H
authors.lastname: Wu
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Xintong
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China. Electronic address: yangliuqing@ujs.edu.cn.
authors.forename: Liuqing
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of the Environment and Safety, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China. Electronic address: wuxy@ujs.edu.cn.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wu
mesh_terms: D000818:Animals; D001921:Brain; D019277:Entropy; D006868:Hydrolysis; D007700:Kinetics; D008956:Models, Chemical; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D013381:Subtilisins / Q000378:metabolism*; D013552:Swine
chemical_list: D010446:Peptide Fragments; D011506:Proteins; D013381:Subtilisins
keywords: Enzymolysis; Enzymolysis model; Kinetics; Porcine cerebral protein; Single-frequency countercurrent and pulsed ultrasound; Thermodynamics