title: Apoptosis transcriptional mechanism of feline infectious peritonitis virus infected cells.
abstract: Apoptosis has been postulated to play an important role during feline infectious peritonitis virus (FIPV) infection; however, its mechanism is not well characterized. This study is focused on apoptosis and transcriptional profiling of FIPV-infected cells following in vitro infection of CRFK cells with FIPV 79-1146 WSU. Flow cytometry was used to determine mode of cell death in first 42 h post infection (hpi). FIPV infected cells underwent early apoptosis at 9 hpi (p < 0.05) followed by late apoptosis at 12 hpi (p < 0.05) and necrosis from 24 hpi (p < 0.05). Then, next generation sequencing was performed on 9 hpi and control uninfected cells by Illumina analyzer. An aggregate of 4546 genes (2229 down-regulated and 2317 up-regulated) from 17 cellular process, 11 molecular functions and 130 possible biological pathways were affected by FIPV. 131 genes from apoptosis cluster (80 down-regulated and 51 up-regulated) along with increase of apoptosis, p53, p38 MAPK, VEGF and chemokines/cytokines signaling pathways were probably involved in apoptosis process. Six of the de-regulated genes expression (RASSF1, BATF2, MAGEB16, PDCD5, TNFα and TRAF2) and TNFα protein concentration were analyzed by RT-qPCR and ELISA, respectively, at different time-points. Up-regulations of both pro-apoptotic (i.e. PDCD5) and anti-apoptotic (i.e. TRAF2) were detected from first hpi and continuing to deregulate during apoptosis process in the infected cells.
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
authors.forename: Ahmad Naqib
authors.initials: AN
authors.lastname: Shuid
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
authors.forename: Nikoo
authors.initials: N
authors.lastname: Safi
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
authors.forename: Amin
authors.initials: A
authors.lastname: Haghani
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.|Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Mehrbod
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
authors.forename: Mohd Syamsul Reza
authors.initials: MS
authors.lastname: Haron
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
authors.forename: Sheau Wei
authors.initials: SW
authors.lastname: Tan
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. aro@upm.edu.my.|Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. aro@upm.edu.my.
authors.forename: Abdul Rahman
authors.initials: AR
authors.lastname: Omar
mesh_terms: D000818:Animals; D017209:Apoptosis; D002371:Cat Diseases / Q000235:genetics* / Q000503:physiopathology / Q000821:virology; D002415:Cats; D018352:Coronavirus Infections / Q000235:genetics / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D016765:Coronavirus, Feline / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics
chemical_list: D016159:Tumor Suppressor Protein p53; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Apoptosis; Feline infectious peritonitis virus; Flow cytometry; Next generation sequencing; RT-qPCR; Transcriptome