title: The chemokine receptor CXCR7 is a critical regulator for the tumorigenesis and development of papillary thyroid carcinoma by inducing angiogenesis in vitro and in vivo.
abstract: Papillary thyroid carcinoma (PTC) is a well-differentiated neoplasm, but it can transfer early to cervical lymph nodes. Accumulating evidences have confirmed the important roles of CXCR7 in tumor cell proliferation, invasion, metastasis, and angiogenesis. Our previous study demonstrated CXCR7 modulated proliferation, apoptosis, and invasion of PTC cells. In this study, we evaluated the effect of expression of CXCR7 in PTC cells on angiogenesis and whether its expression had an influence on the tumor growth of PTC in vivo. We evaluated the effect of CXCR7 on interleukin-8 (IL-8) and vascular endothelial growth factor (VEGF) secretion, angiogenesis, and tumor growth by ELISA, endothelial tube formation assay, and a xenograft tumor model in nude mice. Immunohistochemistry was used to assess expression of CD34 in tumor of mice. In vitro and in vivo studies in PTC cells suggested that the alteration of CXCR7 expression was correlated with angiogenesis and tumor growth. Moreover, CXCR7 mediated the expression of IL-8 and VEGF, which might be involved in the regulation of tumor angiogenesis. These findings suggest that CXCR7 affects the growth of PTC cells and participates in the tumorigenesis of PTC, probably through regulating angiogenesis by the proangiogenic VEGF or IL-8.
authors.affiliation: Department of General Surgery, Shengjing Hospital, China Medical University, No. 36 Sanhao Street, Shenyang, 110004, China.
authors.forename: Hengwei
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, First Affiliated Hospital, China Medical University, Shenyang, 110001, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of General Surgery, Shengjing Hospital, China Medical University, No. 36 Sanhao Street, Shenyang, 110004, China.
authors.forename: Xuyong
authors.initials: X
authors.lastname: Teng
authors.affiliation: Department of General Surgery, Shengjing Hospital, China Medical University, No. 36 Sanhao Street, Shenyang, 110004, China.
authors.forename: Zhangyi
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Shengjing Hospital, China Medical University, No. 36 Sanhao Street, Shenyang, 110004, China.
authors.forename: Chenxi
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Shengjing Hospital, China Medical University, No. 36 Sanhao Street, Shenyang, 110004, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Shengjing Hospital, China Medical University, No. 36 Sanhao Street, Shenyang, 110004, China. liuzhen1973@aliyun.com.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000378:metabolism; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology; D063646:Carcinogenesis / Q000378:metabolism* / Q000473:pathology*; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology*; D002291:Carcinoma, Papillary; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000378:metabolism* / Q000473:pathology; D054387:Receptors, CXCR / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: C501896:ACKR3 protein, human; D043925:Angiogenesis Inducing Agents; D018952:Antigens, CD34; D016209:Interleukin-8; D054387:Receptors, CXCR; D042461:Vascular Endothelial Growth Factor A
keywords: Angiogenesis; CXCR7; Chemokine receptor; Interleukin-8; Thyroid neoplasms; Vascular endothelial growth factor