title: In vitro assessment of environmental stress of persistent organic pollutants on the Indo-Pacific humpback dolphin.
abstract: Persistent organic pollutants (POPs) are detected ubiquitously and are linked to range of adverse health effects. The Indo-Pacific humpback dolphin inhabited the Pearl River Estuary (PRE), China, where high concentrations of POPs have been reported. This study evaluated the threats posed by POPs in the environment to the dolphin using an in vitro system. We selected BNF(β-naphthoflavone) and four POPs (DDTs (dichlorodiphenyltrichloroethanes), CHLs(chlorides), HCHs(hexachlorocyclohexanes) and HCB(hexachlorobenzene)) which had been accumulated in the dolphin with high concentrations to treat the cultured skin fibroblast cells (ScSF cells) of the dolphin, and investigated the expression patterns of the ecological stress biomarkers CYP1A1, AHR and HSP70 in the cell line. The results showed that CYP1A1 was up-regulated after being exposed to different concentrations of BNF, DDTs and HCHs. CHLs, HCHs and HCB promoted AHR expression. HSP70 expression was increased by high concentrations of BNF and DDTs. Moreover, comet assay experiments revealed that DDTs produced higher degree of DNA damage to ScSF cells than other POPs, implying that the Indo-Pacific humpback dolphin in the PRE has been threatened by POPs accumulated in the body, especially by DDTs. Our results provided important information to assess the risk of the Indo-Pacific humpback dolphin raised by environmental POPs in vivo.
authors.affiliation: School of Marine Sciences, Sun Yat-Sen University, Guangdong 510275, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-Sen University, Guangdong 510275, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-Sen University, Guangdong 510275, China.
authors.forename: Kuntong
authors.initials: K
authors.lastname: Jia
authors.affiliation: School of Life Sciences, Sun Yat-Sen University, Guangdong 510275, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Ding
authors.affiliation: School of Life Sciences, Sun Yat-Sen University, Guangdong 510275, China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Marine Sciences, Sun Yat-Sen University, Guangdong 510275, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-Sen University, Guangdong 510275, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-Sen University, Guangdong 510275, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of Marine Sciences, Sun Yat-Sen University, Guangdong 510275, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-Sen University, Guangdong 510275, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-Sen University, Guangdong 510275, China. Electronic address: yimsh@mail.sysu.edu.cn.
authors.forename: Meisheng
authors.initials: M
authors.lastname: Yi
authors.affiliation: School of Marine Sciences, Sun Yat-Sen University, Guangdong 510275, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-Sen University, Guangdong 510275, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-Sen University, Guangdong 510275, China. Electronic address: exwyp@mail.sysu.edu.cn.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D004249:DNA Damage; D004289:Dolphins / Q000378:metabolism*; D004785:Environmental Pollutants / Q000633:toxicity*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics; D013312:Stress, Physiological; D019324:beta-Naphthoflavone / Q000633:toxicity
chemical_list: D004785:Environmental Pollutants; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D018336:Receptors, Aryl Hydrocarbon; D019324:beta-Naphthoflavone; D019363:Cytochrome P-450 CYP1A1
keywords: Biomarker; DNA damage; Persistent organic pollutants; Skin fibroblast cell; Sousa chinensis