title: IL-1β produced by aggressive breast cancer cells is one of the factors that dictate their interactions with mesenchymal stem cells through chemokine production.
abstract: The aim of this work was to understand whether the nature of breast cancer cells could modify the nature of the dialog of mesenchymal stem cells (MSCs) with cancer cells. By treating MSCs with the conditioned medium of metastatic Estrogen-receptor (ER)-negative MDA-MB-231, or non-metastatic ER-positive MCF-7 breast cancer cells, we observed that a number of chemokines were produced at higher levels by MSCs treated with MDA-MB-231 conditioned medium (CM). MDA-MB-231 cells were able to induce NF-κB signaling in MSC cells. This was shown by the use of a NF-kB chemical inhibitor or an IκB dominant negative mutant, nuclear translocation of p65 and induction of NF-κB signature. Our results suggest that MDA-MB-231 cells exert their effects on MSCs through the secretion of IL-1β, that activates MSCs and induces the same chemokines as the MDA-MB-231CM. In addition, inhibition of IL-1β secretion in the MDA-MB-231 cells reduces the induced production of a panel of chemokines by MSCs, as well the motility of MDA-MB-231 cells. Our data suggest that aggressive breast cancer cells secrete IL-1β, which increases the production of chemokines by MSCs.
authors.affiliation: INSERM, U844, U1183, Montpellier, F-34091, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Escobar
authors.affiliation: CNRS, SYS2DIAG, Cap Delta, Montpellier, F-34184, France.|INSERM, U844, U1183, Montpellier, F-34091, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Bouclier
authors.affiliation: INSERM, U844, U1183, Montpellier, F-34091, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Serret
authors.affiliation: Institut Curie, Unité de Pharmacogénomique, Département de Génétique, Paris, 75248, France.
authors.forename: Ivan
authors.initials: I
authors.lastname: Bièche
authors.affiliation: INSERM, U844, U1183, Montpellier, F-34091, France.
authors.forename: Madly
authors.initials: M
authors.lastname: Brigitte
authors.affiliation: INSERM, U844, U1183, Montpellier, F-34091, France.
authors.forename: Andres
authors.initials: A
authors.lastname: Caicedo
authors.affiliation: INSERM, U844, U1183, Montpellier, F-34091, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Sanchez
authors.affiliation: Institut Curie, Unité de Pharmacogénomique, Département de Génétique, Paris, 75248, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Vacher
authors.affiliation: INSERM, U844, U1183, Montpellier, F-34091, France.
authors.forename: Marie-Luce
authors.initials: ML
authors.lastname: Vignais
authors.affiliation: Univercell Biosolutions, Pierre Potier, Toulouse, F-31106, France.|CSA21, Toulouse, F-31100, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Bourin
authors.affiliation: INSERM, U844, U1183, Montpellier, F-34091, France.
authors.forename: David
authors.initials: D
authors.lastname: Geneviève
authors.affiliation: CNRS, SYS2DIAG, Cap Delta, Montpellier, F-34184, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Molina
authors.affiliation: INSERM, U844, U1183, Montpellier, F-34091, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Jorgensen
authors.affiliation: CNRS, SYS2DIAG, Cap Delta, Montpellier, F-34184, France.
authors.forename: Gwendal
authors.initials: G
authors.lastname: Lazennec
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009154:Mutation; D009361:Neoplasm Invasiveness; D019899:Paracrine Communication; D034622:RNA Interference; D015398:Signal Transduction; D013997:Time Factors; D051996:Transcription Factor RelA / Q000378:metabolism; D014162:Transfection; D059016:Tumor Microenvironment
chemical_list: D018925:Chemokines; D017077:Culture Media, Conditioned; D020672:I-kappa B Proteins; C508585:IL1B protein, human; D053583:Interleukin-1beta; C498143:RELA protein, human; D051996:Transcription Factor RelA
keywords: IL-1beta; breast; cancer; chemokines; mesenchymal stem cells