title: An extracellular laccase with potent dye decolorizing ability from white rot fungus Trametes sp. LAC-01.
abstract: A novel laccase was purified from fermentation broth of white rot fungus Trametes sp. LAC-01 using an isolation procedure involving three ion-exchange chromatography steps on DEAE-cellulose, SP-Sepharose, and Q-Sepharose, and one gel-filtration step. The purified enzyme (TSL) was proved as a monomeric protein with a Mr of 59kDa based on SDS-PAGE and FPLC. Partial amino acid sequences were obtained by LC-MS/MS sharing considerably high sequence similarity with that of other laccases. It possessed optimal pH of 2.6 and temperature of 60°C using ABTS as the substrate. The Km of the laccase toward ABTS was estimated to 30.28μM at pH 2.6 and 40°C. TSL manifested considerably high oxidizing activity toward ABTS, but was avoid of degradative activity toward benzidine, caftaric acid, etc. It was effective in the decolorization of phenolic dyes - Bromothymol Blue and Malachite Green with decolorization rate higher than 60% after 24h of incubation. Adjunction of Cu(2+) with the final concentration of 2.0mmol/L significantly activated laccase production with a steady high level of 275.8-282.2U/mL in 96-144h. The high yield and short production period makes Trametes sp. LAC-01 and TSL potentially useful for industrial and environmental application and commercialization.
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Zhuo-Ren
authors.initials: ZR
authors.lastname: Ling
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Wang
authors.affiliation: Department of Fungal Resource, Shandong Agricultural University, Taian, Shandong 271018, China.
authors.forename: Meng-Juan
authors.initials: MJ
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Ning
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Shou-Nan
authors.initials: SN
authors.lastname: Wang
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Ai-Zhen
authors.initials: AZ
authors.lastname: Yang
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China. Electronic address: zhanggq1001@gmail.com.
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China. Electronic address: 20027202@bac.edu.cn.
authors.forename: Xiao-Meng
authors.initials: XM
authors.lastname: Zhao
mesh_terms: D002853:Chromatography, Liquid; D004396:Coloring Agents / Q000737:chemistry*; D003300:Copper / Q000737:chemistry; D021901:DNA, Intergenic; D004789:Enzyme Activation; D004795:Enzyme Stability; D005285:Fermentation; D005658:Fungi / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D042845:Laccase / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D008970:Molecular Weight; D010802:Phylogeny; D013379:Substrate Specificity; D053719:Tandem Mass Spectrometry; D013696:Temperature; D055454:Trametes / Q000145:classification / Q000201:enzymology / Q000235:genetics
chemical_list: D004396:Coloring Agents; D021901:DNA, Intergenic; D003300:Copper; D042845:Laccase
keywords: Dye decolorizing activity; Laccase; Trametes sanguinea