title: Optimization of Fermentation Medium for Extracellular Lipase Production from Aspergillus niger Using Response Surface Methodology.
abstract: Lipase produced by Aspergillus niger is widely used in various industries. In this study, extracellular lipase production from an industrial producing strain of A. niger was improved by medium optimization. The secondary carbon source, nitrogen source, and lipid were found to be the three most influential factors for lipase production by single-factor experiments. According to the statistical approach, the optimum values of three most influential parameters were determined: 10.5 g/L corn starch, 35.4 g/L soybean meal, and 10.9 g/L soybean oil. Using this optimum medium, the best lipase activity was obtained at 2,171 U/mL, which was 16.4% higher than using the initial medium. All these results confirmed the validity of the model. Furthermore, results of the Box-Behnken Design and quadratic models analysis indicated that the carbon to nitrogen (C/N) ratio significantly influenced the enzyme production, which also suggested that more attention should be paid to the C/N ratio for the optimization of enzyme production.
authors.affiliation: Shenzhen Leveking Bio-Engineering Co., Ltd., Guangdong, Shenzhen 518055, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Jia
authors.affiliation: School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yang
authors.affiliation: School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Shenzhen Institute of Technology, Guangdong, Shenzhen 518116, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Shenzhen Leveking Bio-Engineering Co., Ltd., Guangdong, Shenzhen 518055, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Shenzhen Leveking Bio-Engineering Co., Ltd., Guangdong, Shenzhen 518055, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Guo
authors.affiliation: School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong.
authors.forename: Carol Sze Ki
authors.initials: CS
authors.lastname: Lin
authors.affiliation: Shenzhen Leveking Bio-Engineering Co., Ltd., Guangdong, Shenzhen 518055, China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Wang
authors.affiliation: Shenzhen Leveking Bio-Engineering Co., Ltd., Guangdong, Shenzhen 518055, China.
authors.forename: Yunshan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology* / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology*; D007218:Industrial Microbiology / Q000379:methods; D008049:Lipase / Q000096:biosynthesis* / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism
chemical_list: D003470:Culture Media; D002244:Carbon; D008049:Lipase; D009584:Nitrogen