title: Biomimetic Acoustically-Responsive Vesicles for Theranostic Applications.
abstract: In recent years, biomimetic cell membrane-derived particles have emerged as a new class of drug delivery system with advantages of biocompatibility, ease of isolation and long circulation profile. Here we report the development and potential theranostic applications of a new biomimetic acoustically-responsive droplet system derived from mammalian red blood cell membrane (RBCM). We hypothesized that drug-loaded RBCM droplets (RBCMDs) would undergo a transition from liquid (droplets) to gas (bubbles) upon high intensity focused ultrasound (HIFU) insonation, resulting in on-demand drug release. The generated microbubbles could also serve as a contrast agent to enhance ultrasound imaging. As-synthesized RBCMDs exhibited uniform size, good dispersity and preservation of RBCM-associated proteins that prevented uptake by macrophages. Camptothecin (CPT), an anti-cancer drug, was successfully loaded in the RBCMDs with a loading efficiency of 2-3% and an encapsulation efficiency of 62-97%. A short (3 min) exposure to HIFU irradiation triggered release of CPT from the RBCMDs and the physical explosion of droplets damaged nearby cancer cells resulting in significant cell death. In addition, the acoustically vaporized RBCMDs significantly increased the ultrasound echo signal to 30 dB. Lastly, we demonstrated that RBCMDs could be acoustically vaporized in vivo in target tissues, and enhancing ultrasound imaging. Taken together, we have developed a new class of naturally derived RBCMDs which show great potential for future application in remotely triggered drug delivery and ultrasound imaging enhancement.
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 30013, Taiwan.
authors.forename: Chen-Chan
authors.initials: CC
authors.lastname: Hsieh
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 30013, Taiwan.
authors.forename: Shih-Tsung
authors.initials: ST
authors.lastname: Kang
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 30013, Taiwan.
authors.forename: Yee-Hsien
authors.initials: YH
authors.lastname: Lin
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 30013, Taiwan.
authors.forename: Yi-Ju
authors.initials: YJ
authors.lastname: Ho
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 30013, Taiwan.
authors.forename: Chung-Hsin
authors.initials: CH
authors.lastname: Wang
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 30013, Taiwan.
authors.forename: Chih-Kuang
authors.initials: CK
authors.lastname: Yeh
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 30013, Taiwan.
authors.forename: Chien-Wen
authors.initials: CW
authors.lastname: Chang
mesh_terms: D040761:Biomimetic Materials / Q000008:administration & dosage*; D002462:Cell Membrane; D016503:Drug Delivery Systems / Q000379:methods*; D004912:Erythrocytes; D013016:Sound; D000068936:Theranostic Nanomedicine / Q000379:methods*; D014465:Ultrasonics / Q000379:methods*
keywords: biomimetic vesicles.; cancer therapy; red blood cell membrane; remotely triggerable drug delivery; ultrasound theranostics