title: Elucidation of markers for monitoring morphine and its analogs in urine adulterated with pyridinium chlorochromate.
abstract: OBJECTIVE
  Currently, procedures that identify the drugs 'destroyed' in adulterated urine specimens are very limited. This study aimed to determine the effect of pyridinium chlorochromate (PCC) on routine opiate assays and identify reaction products formed. Results/methodology: Opiate-positive urines adulterated with PCC (20 and 100 mM) were analyzed using CEDIA®immunoassay and GC-MS. Urine and water samples spiked with 6-monoacetylmorphine, morphine and its glucuronides (10 µg/ml) and PCC (0.02-100 mM) were monitored with LC-MS, and the products characterized.
  
  
  CONCLUSIONS
  PCC significantly decreased the abundance of morphine, codeine and IS. Adulterated water and urine samples containing 6-monoacetylmorphine, morphine and morphine-3-glucuronide yielded morphinone-3-glucuronide, 7,14-dihydroxy-6-monoacetylmorphine, 7,8-diketo-6-monoacetylmorphine and 7,8-diketo-morphine (tentative assignment). Reaction pathways may be different in the two matrices.
authors.affiliation: Centre for Forensic Science, School of Mathematical and Physical Sciences, University of Technology Sydney (UTS), PO Box 123 Broadway, NSW 2007, Australia.
authors.forename: Susan
authors.initials: S
authors.lastname: Luong
authors.affiliation: Centre for Forensic Science, School of Mathematical and Physical Sciences, University of Technology Sydney (UTS), PO Box 123 Broadway, NSW 2007, Australia.
authors.forename: Unnikrishnan
authors.initials: U
authors.lastname: Kuzhiumparambil
authors.affiliation: Centre for Forensic Science, School of Mathematical and Physical Sciences, University of Technology Sydney (UTS), PO Box 123 Broadway, NSW 2007, Australia.
authors.forename: Shanlin
authors.initials: S
authors.lastname: Fu