title: Metabolic responses to Lactobacillus plantarum contamination or bacteriophage treatment in Saccharomyces cerevisiae using a GC-MS-based metabolomics approach.
abstract: Bacteriophage can be used as a potential alternative agent for controlling Lactobacillus plantarum contamination during bioethanol production. However, how Saccharomyces cerevisiae respond against contaminative L. plantarum or added bacteriophage remains to be fully understood. In this study, gas chromatography-mass spectrometry and a multivariate analysis were employed to investigate the intracellular biochemical changes in S. cerevisiae cells that were elicited by L. plantarum contamination or bacteriophage treatment. The intracellular metabolite profiles originating from different groups were unique and could be distinguished with the aid of principal component analysis. Moreover, partial least-squares-discriminant analysis revealed a group classification and pairwise discrimination, and 13 differential metabolites with variable importance in the projection value greater than 1 were identified. The metabolic relevance of these compounds in the response of S. cerevisiae to L. plantarum contamination or bacteriophage treatment was discussed. Besides generating lactic acid and competing for nutrients or living space, L. plantarum contamination might also inhibit the growth of S. cerevisiae through regulating the glycolysis in S. cerevisiae. Moreover, increased concentrations of monounsaturated fatty acids secondary to bacteriophage treatment might lead to more membrane fluidity and promote the cell viability of S. cerevisiae.
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.|Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, People's Republic of China.
authors.forename: Feng-Xia
authors.initials: FX
authors.lastname: Cui
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
authors.forename: Rui-Min
authors.initials: RM
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
authors.forename: Hua-Qing
authors.initials: HQ
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China. lihaoh@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
mesh_terms: D001435:Bacteriophages / Q000378:metabolism*; D018920:Coculture Techniques; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006019:Glycolysis; D019344:Lactic Acid / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D055432:Metabolomics; D015999:Multivariate Analysis; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D019344:Lactic Acid; D000431:Ethanol
keywords: Bacteriophage; Bioethanol; Lactobacillus plantarum contamination; Metabolomics; Saccharomyces cerevisiae