title: Microfluidics using a thiol-acrylate resin for fluorescence-based pathogen detection assays.
abstract: We demonstrate thiol-acrylate microfluidics prepared via soft lithography for single-step protein immobilization and fluorescence-based pathogen detection. Such microfluidics are formed via room temperature curing, and bonded without oxygen plasma. The background fluorescence of the resin was found to be similar to PDMS for several filter sets. We also show that thiol-acrylate devices are able to bond to gold-coated surfaces, which allows for integration with microfabricated sensors.
authors.affiliation: Institute for Micromanufacturing, Louisiana Tech University, 911 Hergot Ave, Ruston, LA 71272, USA. radadia@latech.edu.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: M P
authors.initials: MP
authors.lastname: Tullier
authors.forename: K
authors.initials: K
authors.lastname: Patel
authors.forename: A
authors.initials: A
authors.lastname: Carranza
authors.forename: J A
authors.initials: JA
authors.lastname: Pojman
authors.forename: A D
authors.initials: AD
authors.lastname: Radadia
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D055910:Antibodies, Immobilized; D004129:Dimethylpolysiloxanes; D013438:Sulfhydryl Compounds; C013830:baysilon