title: Transcriptome immunomodulation of in-feed additives in Atlantic salmon Salmo salar infested with sea lice Caligus rogercresseyi.
abstract: One of the most significant threats to the Chilean salmon aquaculture industry is the ectoparasitic sea louse Caligus rogercresseyi. To cope with sea lice infestations, functional diets have become an important component in strengthening the host immune response. The aim of this study was to evaluate molecular mechanisms activated through immunostimulation by in-feed plant-derived additives in Atlantic salmon infected with sea lice. Herein, a transcriptome-wide sequencing analysis was performed from skin and head kidney tissues, evidencing that the immune response genes were the most variable after the challenge, especially in the head kidney, while other genes involved in metabolism were highly expressed individuals fed with the immunostimulants. Interestingly, defensive enzymes such as Cytochrome p450 and serpins were down-regulated in infested individuals, especially in skin tissue. Additionally, MHC-I and MHC-II genes were differentially expressed after the incorporation of the in-feed additives, giving some cues about the protection mechanisms of plant-derived compound as immunostimulants for infested salmons. This is the first published study that evaluates the transcriptomic response of sea lice-infested Atlantic salmon fed with in-feed additives.
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research, Department of Oceanography, University of Concepción, Concepción, Chile.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Núñez-Acuña
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research, Department of Oceanography, University of Concepción, Concepción, Chile.
authors.forename: Ana Teresa
authors.initials: AT
authors.lastname: Gonçalves
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research, Department of Oceanography, University of Concepción, Concepción, Chile.
authors.forename: Valentina
authors.initials: V
authors.lastname: Valenzuela-Muñoz
authors.affiliation: Ewos Innovation Chile, Camino a Pargua km 57, Colaco km 5, Calbuco, Chile.
authors.forename: Jorge
authors.initials: J
authors.lastname: Pino-Marambio
authors.affiliation: Ewos Innovation Norway, N-4335 Dirdal, Norway.
authors.forename: Simon
authors.initials: S
authors.lastname: Wadsworth
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research, Department of Oceanography, University of Concepción, Concepción, Chile. Electronic address: crisgallardo@udec.cl.
authors.forename: Cristian
authors.initials: C
authors.lastname: Gallardo-Escárate
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002677:Chile; D033342:Copepoda / Q000502:physiology*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D004478:Ectoparasitic Infestations / Q000235:genetics / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology* / Q000469:parasitology; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation; D060226:Head Kidney / Q000469:parasitology; D007113:Immunity, Innate; D056747:Immunomodulation; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D020317:Salmo salar; D012867:Skin / Q000469:parasitology
chemical_list: D000276:Adjuvants, Immunologic; D029941:Fish Proteins; D010936:Plant Extracts
keywords: Caligus rogercresseyi; Head kidney; In-feed additives; RNA-seq; Salmo salar; Skin