title: Cytotoxic constituents of Lasiosphaera fenzlii on different cell lines and the synergistic effects with paclitaxel.
abstract: The fruit body of Lasiosphaera fenzlii was found to show cytotoxicity on cancer cells during a preliminary screening. Repeated column chromatography of the fungal methanol extract resulted in the isolation of six compounds identified as 5α,8α-epidioxy-ergosta-6,22-dien-3β-ol (1), 5α,8α-epidioxy-ergosta-6,9(11),22-trien-3β-ol (2), 5α-ergosta-7,22-dien-3β-ol (3), 5α-ergosta-7,22-dien-3-one (4), ergosta-7,22-dien-3β,5α,6β-triol (5) and 6-dihydroxy-2,3-dihydro-1H-isoindol-1-one (6). The two peroxide compounds, 1 and 2, showed cytotoxic activity and compound 1 was selectively cytotoxic to cancer cells. Furthermore, compound 1 synergised the cytotoxicity of paclitaxel on Hela cells by increasing intracellular accumulation of paclitaxel in cancer cells but not in normal cells.
authors.affiliation: a College of Life Science , Inner Mongolia University , Huhhot , China.
authors.forename: J
authors.initials: J
authors.lastname: Gao
authors.affiliation: a College of Life Science , Inner Mongolia University , Huhhot , China.
authors.forename: L W
authors.initials: LW
authors.lastname: Wang
authors.affiliation: b Cancer Research Center , Liaoning Medical University , Jinzhou , China.
authors.forename: H C
authors.initials: HC
authors.lastname: Zheng
authors.affiliation: a College of Life Science , Inner Mongolia University , Huhhot , China.
authors.forename: A
authors.initials: A
authors.lastname: Damirin
authors.affiliation: a College of Life Science , Inner Mongolia University , Huhhot , China.
authors.forename: C M
authors.initials: CM
authors.lastname: Ma
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D004875:Ergosterol / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D054833:Isoindoles / Q000008:administration & dosage / Q000494:pharmacology; D017239:Paclitaxel / Q000008:administration & dosage
chemical_list: C503471:5alpha,8alpha-epidioxyergosta-6,22-dien-3-beta-ol; D054833:Isoindoles; C417570:ergosta-7,22-dien-3-one; D017239:Paclitaxel; D004875:Ergosterol
keywords: Lasiosphaera fenzlii; constituents; cytotoxicity; ergosterol peroxide; paclitaxel; synergistic effect