title: Perturbed meibomian gland and tarsal plate morphogenesis by excess TGFα in eyelid stroma.
abstract: Transforming growth factor alpha (TGFα) belongs to the epidermal growth factor (EGF) family and is known to play an important role during eyelid morphogenesis. In this study, we showed that ectopic expression of TGFα in the stroma of Kera-rtTA/tet-O-TGFα bitransgenic mice results in precocious eye opening, abnormal morphogenesis of the meibomian gland, tendon and tarsal plate malformation and epithelium hyperplasia. TGFα did not change proliferation and differentiation of meibocytes, but promoted proliferation and inhibited differentiation of the tarsal plate tenocytes. These results suggest that proper formation of the tendon and tarsal plate in the mouse eyelid is required for normal morphogenesis of the meibomian gland.
authors.affiliation: Department of Ophthalmology, University of Cincinnati, OH 45267, United States; Eye Institute of Xiamen University, Xiamen, Fujian 361102, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Dong
authors.affiliation: Department of Ophthalmology, University of Cincinnati, OH 45267, United States.
authors.forename: Chia-Yang
authors.initials: CY
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology, University of Cincinnati, OH 45267, United States.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Ophthalmology, University of Cincinnati, OH 45267, United States.
authors.forename: Yujin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Eye Institute of Xiamen University, Xiamen, Fujian 361102, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, University of Cincinnati, OH 45267, United States.
authors.forename: Mindy
authors.initials: M
authors.lastname: Call
authors.affiliation: Department of Ophthalmology, University of Cincinnati, OH 45267, United States.
authors.forename: Liyun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Eye Institute of Xiamen University, Xiamen, Fujian 361102, People's Republic of China.
authors.forename: Yongxiong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Eye Institute of Xiamen University, Xiamen, Fujian 361102, People's Republic of China. Electronic address: zuguoliu@xmu.edu.cn.
authors.forename: Zuguo
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology, University of Cincinnati, OH 45267, United States. Electronic address: kaoww@uc.edu.
authors.forename: Winston W Y
authors.initials: WW
authors.lastname: Kao
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D005143:Eyelids / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D006652:Histological Techniques; D007150:Immunohistochemistry; D008537:Meibomian Glands / Q000002:abnormalities* / Q000196:embryology; D051379:Mice; D008822:Mice, Transgenic; D009024:Morphogenesis / Q000502:physiology*; D016133:Polymerase Chain Reaction; D013710:Tendons / Q000166:cytology / Q000196:embryology; D016211:Transforming Growth Factor alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D016211:Transforming Growth Factor alpha
keywords: ECM; Eyelid development; Eyelid tendon; Meibomian gland morphogenesis; TGFα; Tarsal plate