title: SNPs in candidate genes MX dynamin-like GTPase and chemokine (C-C motif) receptor-5 are associated with ovine pulmonary adenocarcinoma progression in Latxa sheep.
abstract: Ovine pulmonary adenocarcinoma (OPA) is a contagious lung cancer in sheep caused by Jaagsiekte sheep retrovirus (JSRV). OPA is present in many sheep-rearing countries causing economic and welfare issues, as currently no efficient vaccines or treatments are available. Breed differences suggest a host genetic component may influence the pathogenesis of OPA, but so far few genes have been identified. In this work, a genetic association study was carried out in Latxa dairy sheep which were classified as cases/controls based on the presence/absence of OPA lung tumours. Candidate genes included cytokines and a receptor and innate immunity genes. After SNPs in the candidate genes were identified, the distribution of alleles in cases and controls was compared by means of logistic regression analyses at the allelic, genotypic and haplotypic levels. The association analysis showed that several candidate genes were significantly associated with resistance or susceptibility to OPA; two of the candidates, CCR5 and MX1, remained significantly associated with resistance and susceptibility respectively, even after Bonferroni correction.
authors.affiliation: Genetics, Physical Anthropology and Animal Physiology Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48080, Bilbao, Bizkaia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Larruskain
authors.affiliation: Genetics, Physical Anthropology and Animal Physiology Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48080, Bilbao, Bizkaia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Esparza-Baquer
authors.affiliation: Animal Health Department, NEIKER-Tecnalia, 48160, Derio, Bizkaia, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Minguijón
authors.affiliation: Animal Health Department, NEIKER-Tecnalia, 48160, Derio, Bizkaia, Spain.
authors.forename: R A
authors.initials: RA
authors.lastname: Juste
authors.affiliation: Genetics, Physical Anthropology and Animal Physiology Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48080, Bilbao, Bizkaia, Spain.
authors.forename: B M
authors.initials: BM
authors.lastname: Jugo
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics; D005787:Gene Frequency; D056726:Genetic Association Studies; D005819:Genetic Markers; D005838:Genotype; D006239:Haplotypes; D022141:Jaagsiekte sheep retrovirus; D008175:Lung Neoplasms / Q000235:genetics* / Q000662:veterinary / Q000821:virology; D064547:Myxovirus Resistance Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011648:Pulmonary Adenomatosis, Ovine / Q000235:genetics* / Q000821:virology; D019713:Receptors, CCR5 / Q000235:genetics*; D012756:Sheep; D034561:Sheep, Domestic / Q000235:genetics* / Q000821:virology; D051193:Toll-Like Receptors / Q000235:genetics
chemical_list: D016207:Cytokines; D005819:Genetic Markers; D064547:Myxovirus Resistance Proteins; D019713:Receptors, CCR5; D051193:Toll-Like Receptors
keywords: association study; cytokines; immune response genes; innate immunity; ovine pulmonary adenocarcinoma