title: Hypnocyclicus thermotrophus gen. nov., sp. nov. isolated from a microbial mat in a hydrothermal vent field.
abstract: The bacterial strain, IR-2T, was isolated from a microbial mat sampled near a hydrothermal vent in the Greenland Sea. Phylogenetic analysis, based on the 16S rRNA gene, showed that the closest relatives of IR-2T were Ilyobacter tartaricus, Ilyobacter insuetus, Propionigenium modestum and Fusobacterium varium (91 % 16S rRNA gene sequence similarity). The cells of the novel strain were Gram-stain-negative and pleomorphic; changing from long motile rods to non-motile ring structures during the growth cycle. Growth occurred at 20-55 °C (optimally at 48 °C), with 1-6 % (w/v) NaCl (optimally with 2 %), and at pH 5.3-8.0 (optimally at pH 6.0-8.0). The strain had obligate fermentative growth on various sugars and yeast extract. The DNA G+C content of strain IR-2T was 25.7 mol%. The cell sugars comprised mainly ribose, mannose and glucose, while the main polar lipids were glycolipids, phospholipids, phosphatidylglycerol and diphosphatidylglycerol. The fatty acid content of strain IR-2 was dominated by saturated and unsaturated iso-branched or anteiso-branched forms. Strain IR-2 represents a novel genus and species, for which the name Hypnocyclicus thermotrophus gen. nov., sp. nov. is proposed. The type strain is IR-2T ( = DSM 100055 = JCM 30901).
authors.affiliation: Center for Geobiology, University of Bergen, Allégaten 41, 5007 Bergen, Norway.|Department of Biology, University of Bergen, Thormøhlensgate 53A/B, 5006 Bergen, Norway.
authors.forename: Irene
authors.initials: I
authors.lastname: Roalkvam
authors.affiliation: Department of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology, HKI, Beutenbergstrasse 11a, 07745 Jena, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Bredy
authors.affiliation: Center for Geobiology, University of Bergen, Allégaten 41, 5007 Bergen, Norway.|Department of Earth Science, University of Bergen, Allégaten 41, 5007 Bergen, Norway.
authors.forename: Tamara
authors.initials: T
authors.lastname: Baumberger
authors.affiliation: Center for Geobiology, University of Bergen, Allégaten 41, 5007 Bergen, Norway.|Department of Earth Science, University of Bergen, Allégaten 41, 5007 Bergen, Norway.
authors.forename: Rolf-B
authors.initials: RB
authors.lastname: Pedersen
authors.affiliation: Center for Geobiology, University of Bergen, Allégaten 41, 5007 Bergen, Norway.|Department of Biology, University of Bergen, Thormøhlensgate 53A/B, 5006 Bergen, Norway.
authors.forename: Ida Helene
authors.initials: IH
authors.lastname: Steen
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D045182:Fusobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006017:Glycolipids / Q000737:chemistry; D060086:Hydrothermal Vents / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S