title: Rapid detection of cefotaxime-resistant Escherichia coli by LC-MS.
abstract: Antibiotic resistance is an unsolved healthcare problem with increasing impact on patient management in the last years. In particular, multidrug resistance among Gram-negative bacterial strains has become the most pressing challenge. In order to deliver the most efficacious antimicrobial therapy with minimum delay, rapid diagnostic tests are required in order to detect multidrug resistant pathogens early during infection. In line with these efforts, we have developed a mass spectrometry-based assay for the rapid determination of ampicillin and cefotaxime resistance. The assay quantifies beta-lactamase activities towards ampicillin and cefotaxime within a turnaround time of 150 min, which is substantially faster than classical susceptibility testing.
authors.affiliation: Institute for Clinical Chemistry, Medical Faculty Mannheim of Heidelberg University, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Burrer
authors.affiliation: Institute for Clinical Chemistry, Medical Faculty Mannheim of Heidelberg University, Germany. Electronic address: peter.findeisen@medma.uni-heidelberg.de.
authors.forename: P
authors.initials: P
authors.lastname: Findeisen
authors.affiliation: Institute for Clinical Chemistry, Medical Faculty Mannheim of Heidelberg University, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Jäger
authors.affiliation: Institute for Medical Laboratory Diagnostics, Center for Clinical and Translational Research, HELIOS Klinikum Wuppertal, Witten/Herdecke University, Witten, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Ghebremedhin
authors.affiliation: Institute for Clinical Chemistry, Medical Faculty Mannheim of Heidelberg University, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Grundt
authors.affiliation: Institute for Medical Laboratory Diagnostics, Center for Clinical and Translational Research, HELIOS Klinikum Wuppertal, Witten/Herdecke University, Witten, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Ahmad-Nejad
authors.affiliation: Institute for Medical Microbiology and Hygiene, Medical Faculty Mannheim of the University of Heidelberg, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Miethke
authors.affiliation: Institute for Clinical Chemistry, Medical Faculty Mannheim of Heidelberg University, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Neumaier
mesh_terms: D000667:Ampicillin / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001431:Bacteriological Techniques / Q000379:methods; D002439:Cefotaxime / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D013997:Time Factors; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D000667:Ampicillin; D001618:beta-Lactamases; D002439:Cefotaxime
keywords: Antibiotic susceptibility testing; Beta-lactamase; E. coli; Mass spectrometry