title: Benzo(a)pyrene inhibits migration and invasion of extravillous trophoblast HTR-8/SVneo cells via activation of the ERK and JNK pathway.
abstract: Benzo(a)pyrene (BaP) is a persistent organic pollutant (POP) that is a serious threat to human health. Numerous studies have shown that BaP causes adverse effects in pregnancy, but the mechanism remains unclear. The moderate invasion of trophoblast cells into the endometrium is an important factor during successful embryo implantation. The aim of this study was to investigate the effect and mechanism of BaP on the invasion and migration of trophoblast cells. HTR-8/SVneo cells were treated with different concentrations (1, 5, 10, 25, 50 and 100 μM) of BaP. The invasion and migration of HTR-8/SVneo cells were observed after BaP treatment. The protein levels related to migration and invasion was detected by Western blot. The results confirmed that BaP inhibits the migration and invasion of extravillous trophoblast HTR-8/SVneo cells. Further investigations indicated that the protein levels of MMP-2, MMP-9 and E-cadherin in HTR-8/SVneo cells were changed by BaP treatment. Moreover, the data demonstrated that BaP activated the MAPK signaling pathway. Pretreatment with specific inhibitors of MAPK rescued BaP-induced change in the migration and invasion of HTR-8/SVneo cells. Taken together, our results indicated that BaP inhibits invasion and the migration of HTR-8/SVneo cells, which might cause a failure in early pregnancy. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Liu
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Yingxiong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Cha
authors.initials: C
authors.lastname: Shen
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Junlin
authors.initials: J
authors.lastname: He
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Rufei
authors.initials: R
authors.lastname: Gao
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001564:Benzo(a)pyrene / Q000633:toxicity*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D020935:MAP Kinase Signaling System; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D010920:Placenta / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D015398:Signal Transduction; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects*
chemical_list: D015820:Cadherins; D001564:Benzo(a)pyrene; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: BaP; HTR-8/SVneo cell; MAPK signaling pathway; invasion; migration