title: Effect of phenolic compounds from pretreated sugarcane bagasse on cellulolytic and hemicellulolytic activities.
abstract: This work shows both cellulases and hemicellulases are inhibited and deactivated by water-soluble and acetone extracted phenolics from sugarcane bagasse pretreated at 10% (w/v) for 30 min in liquid hot water at 180 or 200°C. The dissolved phenolics in vacuum filtrate increased from 1.4 to 2.4 g/L as temperature increased from 180 to 200°C. The suppression of cellulose and hemicellulose hydrolysis by phenolics is dominated by deactivation of the β-glucosidase or β-xylosidase components of cellulase and hemicellulase enzyme by acetone extract at 0.2-0.65 mg phenolics/mg enzyme protein and deactivation of cellulases and hemicellulases by the water soluble components in vacuum filtrate at 0.05-2mg/mg. Inhibition was a function of the type of enzyme and the manner in which the phenolics were extracted from the bagasse.
authors.affiliation: Laboratory of Renewable Resources Engineering, Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907-2032, United States; Laboratory of Microbiology and Cellular Biology, Biology Department, School of Philosophy, Sciences and Literature of Ribeirão Preto, São Paulo University, Ribeirão Preto, SP 14040-901, Brazil.
authors.forename: Michele
authors.initials: M
authors.lastname: Michelin
authors.affiliation: Laboratory of Renewable Resources Engineering, Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907-2032, United States.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Ximenes
authors.affiliation: Laboratory of Microbiology and Cellular Biology, Biology Department, School of Philosophy, Sciences and Literature of Ribeirão Preto, São Paulo University, Ribeirão Preto, SP 14040-901, Brazil.
authors.forename: Maria
authors.initials: M
authors.lastname: de Lourdes Teixeira de Moraes Polizeli
authors.affiliation: Laboratory of Renewable Resources Engineering, Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907-2032, United States. Electronic address: ladisch@purdue.edu.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Ladisch
mesh_terms: D000096:Acetone / Q000737:chemistry; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D005374:Filtration; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006358:Hot Temperature; D006868:Hydrolysis; D010636:Phenols / Q000494:pharmacology*; D031786:Saccharum / Q000737:chemistry*; D014618:Vacuum; D014867:Water / Q000494:pharmacology
chemical_list: D010636:Phenols; D014867:Water; D000096:Acetone; D002482:Cellulose; C027433:bagasse; D006026:Glycoside Hydrolases; C023305:hemicellulase; D002480:Cellulase
keywords: Deactivation; Enzyme; Inhibition; Phenolic compounds; Sugarcane bagasse