title: Metabolite Profiling of Diverse Rice Germplasm and Identification of Conserved Metabolic Markers of Rice Roots in Response to Long-Term Mild Salinity Stress.
abstract: The sensitivity of rice to salt stress greatly depends on growth stages, organ types and cultivars. Especially, the roots of young rice seedlings are highly salt-sensitive organs that limit plant growth, even under mild soil salinity conditions. In an attempt to identify metabolic markers of rice roots responding to salt stress, metabolite profiling was performed by ¹H-NMR spectroscopy in 38 rice genotypes that varied in biomass accumulation under long-term mild salinity condition. Multivariate statistical analysis showed separation of the control and salt-treated rice roots and rice genotypes with differential growth potential. By quantitative analyses of ¹H-NMR data, five conserved salt-responsive metabolic markers of rice roots were identified. Sucrose, allantoin and glutamate accumulated by salt stress, whereas the levels of glutamine and alanine decreased. A positive correlation of metabolite changes with growth potential and salt tolerance of rice genotypes was observed for allantoin and glutamine. Adjustment of nitrogen metabolism in rice roots is likely to be closely related to maintain the growth potential and increase the stress tolerance of rice.
authors.affiliation: Environmental Risk and Welfare Research Team, Korea Basic Science Institute, Seoul 02855, Korea. nammh@kbsi.re.kr.
authors.forename: Myung Hee
authors.initials: MH
authors.lastname: Nam
authors.affiliation: Omics System Research Team, Korea Basic Science Institute, Seoul 03759, Korea. bej@kbsi.re.kr.
authors.forename: Eunjung
authors.initials: E
authors.lastname: Bang
authors.affiliation: Molecular Breeding Division, National Academy of Agricultural Science, Jeonju 565-851, Korea. trkwon@korea.kr.
authors.forename: Taek Yun
authors.initials: TY
authors.lastname: Kwon
authors.affiliation: Environmental Risk and Welfare Research Team, Korea Basic Science Institute, Seoul 02855, Korea. uran115@naver.com.
authors.forename: Yuran
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Protein Structure Team, Korea Basic Science Institute, Cheongju 28119, Korea. keh@kbsi.re.kr.
authors.forename: Eun Hee
authors.initials: EH
authors.lastname: Kim
authors.affiliation: Environmental Risk and Welfare Research Team, Korea Basic Science Institute, Seoul 02855, Korea. kw.cho253@gmail.com.
authors.forename: Kyungwon
authors.initials: K
authors.lastname: Cho
authors.affiliation: Department of Molecular Biology, Institute of Nanosensor and Biotechnology, Dankook University, Yongin-si, Gyeonggi-do 448-701, Korea. parkwj@dankook.ac.kr.
authors.forename: Woong June
authors.initials: WJ
authors.lastname: Park
authors.affiliation: Molecular Breeding Division, National Academy of Agricultural Science, Jeonju 565-851, Korea. bgkimpeace@korea.kr.
authors.forename: Beom-Gi
authors.initials: BG
authors.lastname: Kim
authors.affiliation: Molecular Breeding Division, National Academy of Agricultural Science, Jeonju 565-851, Korea. isyoon@korea.kr.
authors.forename: In Sun
authors.initials: IS
authors.lastname: Yoon
mesh_terms: D015415:Biomarkers; D005838:Genotype; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D012275:Oryza / Q000502:physiology*; D018517:Plant Roots / Q000502:physiology*; D066244:Proton Magnetic Resonance Spectroscopy; D054712:Salinity; D055049:Salt Tolerance; D013312:Stress, Physiological
chemical_list: D015415:Biomarkers
keywords: 1H-NMR; metabolite markers; rice germplasm; root; salt stress