title: Lipidomic Analysis of Chlamydomonas reinhardtii under Nitrogen and Sulfur Deprivation.
abstract: Chlamydomonas reinhardtii accumulates lipids under complete nutrient starvation conditions while overall growth in biomass stops. In order to better understand biochemical changes under nutrient deprivation that maintain production of algal biomass, we used a lipidomic assay for analyzing the temporal regulation of the composition of complex lipids in C. reinhardtii in response to nitrogen and sulfur deprivation. Using a chip-based nanoelectrospray direct infusion into an ion trap mass spectrometer, we measured a diversity of lipid species reported for C. reinhardtii, including PG phosphatidylglycerols, PI Phosphatidylinositols, MGDG monogalactosyldiacylglycerols, DGDG digalactosyldiacylglycerols, SQDG sulfoquinovosyldiacylglycerols, DGTS homoserine ether lipids and TAG triacylglycerols. Individual lipid species were annotated by matching mass precursors and MS/MS fragmentations to the in-house LipidBlast mass spectral database and MS2Analyzer. Multivariate statistics showed a clear impact on overall lipidomic phenotypes on both the temporal and the nutrition stress level. Homoserine-lipids were found up-regulated at late growth time points and higher cell density, while triacyclglycerols showed opposite regulation of unsaturated and saturated fatty acyl chains under nutritional deprivation.
authors.affiliation: Zhong Yuan Academy of Biological Medicine, Liaocheng People's Hospital/Affiliated Liaocheng Hospital, Taishan Medical University, 67 Dong Chang Xi Lu, Liaocheng, Shandong, 252000, P. R. China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Marine Science, College of Marine Science & Engineering, Tianjin University of Science & Technology 29, the 13th St., TEDA, Tianjin, 300457, P. R. China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Song
authors.affiliation: UC Davis Genome Center- Metabolomics, Davis, California 95616, United States of America.
authors.forename: Tobias
authors.initials: T
authors.lastname: Kind
authors.affiliation: UC Davis Genome Center- Metabolomics, Davis, California 95616, United States of America.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Genedata Inc, Waltham, Massachusetts, United States of America.
authors.forename: Jens
authors.initials: J
authors.lastname: Hoefkens
authors.affiliation: UC Davis Genome Center- Metabolomics, Davis, California 95616, United States of America; King Abdulaziz University, Faculty of Science, Biochemistry Department, PO Box 80203, Jeddah 21589, Saudi Arabia.
authors.forename: Oliver
authors.initials: O
authors.lastname: Fiehn
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000254:growth & development / Q000378:metabolism*; D008055:Lipids / Q000032:analysis*; D009584:Nitrogen / Q000172:deficiency*; D013455:Sulfur / Q000172:deficiency*; D053719:Tandem Mass Spectrometry
chemical_list: D008055:Lipids; D013455:Sulfur; D009584:Nitrogen