title: Inhibition of Diethylnitrosamine-Induced Hepatocarcinogenesis in Mice by a High Dietary Protein Intake.
abstract: Epidemiological and experimental evidence supports the key role of diet in the development of many types of cancer. Recent studies have suggested that dietary modifications may be beneficial for individuals at high risk for hepatocellular carcinoma (HCC). In this study, we investigated the effect of a high-protein (HP; 20% casein) dietondiethylnitrosamine (DEN)-induced hepatocarcinogenesis. Mice were given free access to water with 30 μg/ml DEN and fed a normal or HP diet for 22 wk. The results showed mice consuming HP diets had reduced mortality rates and body weights and lower hepatic enzyme activity compared to DEN-treated mice on a normal diet. HP consumption also promoted collagen accumulation in the liver, and reduced numbers of proliferating hepatocytes and infiltrating inflammatory cells, as well as decreased expression of inflammatory factor interleukin-1β, and nuclear factor κB activation. These data indicate that HP diets can inhibit DEN-induced hepatocarcinogenesis via suppression of the inflammatory response and provide a new evidence for the dietary management of clinical patients with hepatocellular carcinoma.
authors.affiliation: a Experimental Animal Research Center, Zhejiang Chinese Medical University , Hangzhou , China.
authors.forename: Qiyang
authors.initials: Q
authors.lastname: Shou
authors.affiliation: a Experimental Animal Research Center, Zhejiang Chinese Medical University , Hangzhou , China.
authors.forename: Fangming
authors.initials: F
authors.lastname: Chen
authors.affiliation: a Experimental Animal Research Center, Zhejiang Chinese Medical University , Hangzhou , China.
authors.forename: Yueqin
authors.initials: Y
authors.lastname: Cai
authors.affiliation: b Department of Orthopaedic Surgery , The First Affiliated Hospital of Zhejiang Chinese Medical University , Hangzhou , China.
authors.forename: Shanxin
authors.initials: S
authors.lastname: Zhang
authors.affiliation: a Experimental Animal Research Center, Zhejiang Chinese Medical University , Hangzhou , China.
authors.forename: Jue
authors.initials: J
authors.lastname: Tu
authors.affiliation: a Experimental Animal Research Center, Zhejiang Chinese Medical University , Hangzhou , China.
authors.forename: Lizong
authors.initials: L
authors.lastname: Zhang
authors.affiliation: a Experimental Animal Research Center, Zhejiang Chinese Medical University , Hangzhou , China.
authors.forename: Dejun
authors.initials: D
authors.lastname: Wang
authors.affiliation: c Central Laboratory, The Second Clinical Medical College, Zhejiang Chinese Medical University , Hangzhou , China.
authors.forename: Jianchao
authors.initials: J
authors.lastname: Wang
authors.affiliation: a Experimental Animal Research Center, Zhejiang Chinese Medical University , Hangzhou , China.
authors.forename: Minli
authors.initials: M
authors.lastname: Chen
authors.affiliation: c Central Laboratory, The Second Clinical Medical College, Zhejiang Chinese Medical University , Hangzhou , China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Fu
mesh_terms: D000818:Animals; D002364:Caseins / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D004044:Dietary Proteins / Q000737:chemistry / Q000494:pharmacology*; D004052:Diethylnitrosamine / Q000633:toxicity*; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D053583:Interleukin-1beta / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000178:diet therapy* / Q000401:mortality / Q000473:pathology; D008297:Male; D008809:Mice, Inbred C3H; D016328:NF-kappa B / Q000378:metabolism; D015996:Survival Rate
chemical_list: D002364:Caseins; D004044:Dietary Proteins; D053583:Interleukin-1beta; D016328:NF-kappa B; D004052:Diethylnitrosamine; D003094:Collagen