title: Diagnostic significance of S100A2 and S100A6 levels in sera of patients with non-small cell lung cancer.
abstract: Biochemical markers play a significant role in the diagnosis of lung cancer. Recent studies have demonstrated a link involving S100 Calcium Binding Proteins (S100A2, S100A6) and non-small cell lung cancer (NSCLC), but the expediency of their serum levels in NSCLC has not been established. In this study, we evaluate the potential of serum S100A2 and S100A6 levels as diagnostic markers for NSCLC. Enzyme-linked immunosorbent assay (ELISA) was performed to detect the levels of S100A2 and S100A6 in 141 NSCLC patients and 150 healthy subjects. Serum levels of the two proteins in patients with NSCLC were higher compared to healthy controls (P = 0.0002 for S100A2 and P < 0.0001 for S100A6). Moreover, the levels of S100A2 and S100A6 were higher in the sera of stage I/II NSCLC patients compared to healthy controls with P = 0.01 and <0.0001, respectively. Receiver operating characteristic (ROC) analysis showed that S100A2 could distinguish NSCLC patients from healthy controls (AUC = 0.646), and S100A6 could also identify NSCLC (AUC = 0.668). Meanwhile, these two proteins showed notable capabilities for distinguishing stage I/II NSCLC from healthy controls (AUC = 0.708 for S100A2 and AUC = 0.702 for S100A6). Our results indicate that serum levels of S100A2 and S100A6 are significantly elevated in early stage NSCLC and may have the potential for NSCLC biomarker. Further studies with large sample population would help validate our findings.
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of School of Medicine, Xi'an Jiaotong University, 277 Yanta West Street, Xi'an, 710061, People's Republic of China.|Department of Respiratory Medicine, Xi'an No.4 Hospital, Xi'an, 710004, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of School of Medicine, Xi'an Jiaotong University, 277 Yanta West Street, Xi'an, 710061, People's Republic of China.
authors.forename: Yiqian
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of School of Medicine, Xi'an Jiaotong University, 277 Yanta West Street, Xi'an, 710061, People's Republic of China.|Department of Internal Medicine, Life Guard Hospital, Biratnagar, Nepal.|S.R. Laboratory and Diagnostic Center, Biratnagar, Nepal.
authors.forename: Asmitananda
authors.initials: A
authors.lastname: Thakur
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of School of Medicine, Xi'an Jiaotong University, 277 Yanta West Street, Xi'an, 710061, People's Republic of China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of School of Medicine, Xi'an Jiaotong University, 277 Yanta West Street, Xi'an, 710061, People's Republic of China.
authors.forename: Tian
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of School of Medicine, Xi'an Jiaotong University, 277 Yanta West Street, Xi'an, 710061, People's Republic of China.
authors.forename: Tianjun
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of School of Medicine, Xi'an Jiaotong University, 277 Yanta West Street, Xi'an, 710061, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of School of Medicine, Xi'an Jiaotong University, 277 Yanta West Street, Xi'an, 710061, People's Republic of China. chenmingwei64@hotmail.com.
authors.forename: Mingwei
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of School of Medicine, Xi'an Jiaotong University, 277 Yanta West Street, Xi'an, 710061, People's Republic of China. jessie_renhui@aliyun.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Ren
mesh_terms: D000328:Adult; D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood*; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000175:diagnosis*; D018797:Cell Cycle Proteins / Q000097:blood*; D002630:Chemotactic Factors / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D002630:Chemotactic Factors; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C098997:S100A2 protein, human; C055351:S100A6 protein, human
keywords: Biomarkers; Calcium binding protein; Non-small cell lung cancer; S100A2; S100A6