title: Sampling of Candida albicans and Candida tropicalis by Langerin-positive dendritic cells in mouse Peyer's patches.
abstract: Members of the Candida genus, including C. albicans and C. tropicalis are opportunistic fungal pathogens that are increasingly associated with gastrointestinal infections and inflammatory bowel diseases. In healthy populations, however, C. albicans and C. tropicalis are considered benign members of the mycobiome, and are presumably kept in check by the mucosal immune system. In this study, we demonstrate in mice that C. albicans and C. tropicalis are sampled by Peyer's patch (PP) dendritic cells (DCs). Uptake into gut-associated lymphoid tissues occurred rapidly and was at least partly M cell-dependent. C. albicans and C. tropicalis preferentially localized in (and persisted within) a recently identified sub- population of Peyer's patch DCs distinguished by their expression of the C-type lectin receptor, Langerin. This study is the first to identify a subset of PP DCs capable of sampling Candida species.
authors.affiliation: Department of Biomedical Sciences, University at Albany, School of Public Health, Albany, NY, USA; Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, NY, USA. Electronic address: magdia.dejesus@health.ny.gov.
authors.forename: Magdia
authors.initials: M
authors.lastname: De Jesus
authors.affiliation: Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, NY, USA; Dutchess Community College, Poughkeepsie, NY, USA.
authors.forename: Adam E
authors.initials: AE
authors.lastname: Rodriguez
authors.affiliation: Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Gary R
authors.initials: GR
authors.lastname: Ostroff
authors.affiliation: Department of Biomedical Sciences, University at Albany, School of Public Health, Albany, NY, USA; Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, NY, USA.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Mantis
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000276:immunology* / Q000378:metabolism; D018938:Caco-2 Cells; D002176:Candida albicans / Q000276:immunology* / Q000502:physiology; D041022:Candida tropicalis / Q000276:immunology* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D019073:HT29 Cells; D054884:Host-Pathogen Interactions / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007114:Immunization / Q000379:methods; D037181:Lectins, C-Type / Q000276:immunology* / Q000378:metabolism; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D008297:Male; D037241:Mannose-Binding Lectins / Q000276:immunology* / Q000378:metabolism; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D047071:beta-Glucans / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000954:Antigens, Surface; C498497:Cd207 protein, mouse; D016207:Cytokines; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D047071:beta-Glucans; D010047:Ovalbumin
keywords: C-type lectins; Dendritic cells; Diphtheria toxin; Langerin; Peyer’s patch