title: Establishing a reentry procedure for human immunodeficiency virus screening-reactive donors in China.
abstract: BACKGROUND
  Screening of blood donors for antibody to human immunodeficiency virus Types 1 and 2 (anti-HIV-1/2) and/or HIV nucleic acid test (NAT) is a well-established venue to prevent HIV transfusion-transmitted disease. However, with the current available technologies, HIV testing may result in donor loss due to false-positive results. This study intended to establish a donor reentry procedure for HIV screening-reactive donors in China.
  
  
  METHODS
  From September 1, 2013, to August 31, 2014, a total of 465 donors from 14 Chinese blood centers were enrolled in this study. Enrollment criteria include all donors who were screened reactive or belonged to the "gray zone" by enzyme-linked immunosorbent assay and/or reactive by NAT when tested at the local blood centers. All donor samples were sent to a central HIV confirmation laboratory where anti-HIV-1/2 and HIV individual-donation NATs were conducted. If the results were reactive for anti-HIV-1/2, then the samples were tested with a recombinant immunoblot assay.
  
  
  RESULTS
  Based on the repeat testing at the central HIV confirmation laboratory 8 or 16 weeks after the study, 252 donors of 465 (54.2%) who completed the study could be classified in two categories for HIV status: 45 (18%) true positive and 207 (82%) false positive. A total of 213 of 465 (45.8%) donors were lost on follow-up and, thus, their HIV status cannot be determined with certainty. Based on these data, a donor reentry procedure was proposed.
  
  
  CONCLUSIONS
  Based on our proposed donor reentry procedure for HIV screening-reactive donors, a majority of screening-positive donors (82%, 207/252) can be reentered safely.
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, Sichuan, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: Xinjiang Yili Blood Center, Yili, Xinjiaing, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xu
authors.affiliation: Shanxi Changzhi Blood Center, Changzhi, Shanxi, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: Liaoning Dalian Blood Center, Dalian, Liaoning, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zang
authors.affiliation: Anhui Blood Center, Hefei, Anhui Province, China.
authors.forename: Weifang
authors.initials: W
authors.lastname: Cheng
authors.affiliation: Fujian Blood Center, Fuzhou, Fujian Province, China.
authors.forename: Hongkeng
authors.initials: H
authors.lastname: Lin
authors.affiliation: Jiangsu Blood Center, Nanjing, Jiangsu, China.
authors.forename: Shaowen
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Sichuan Nanchong Blood Center, Nanchong, Sichuan Province, China.
authors.forename: Binghai
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Hunan Hengyang Blood Center, Hengyang, Hunan, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Duan
authors.affiliation: Anhui Suzhou Blood Center, Suzhou, Anhui, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Li
authors.affiliation: Sichuan Mianyang Blood Center, Mianyang, Sichuan Province, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Huang
authors.affiliation: Henan Kaifeng Blood Center, Kaifeng, Henan Province, China.
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Sichuan Guangan Blood Center, Guangan, Sichuan, China.
authors.forename: Chengyan
authors.initials: C
authors.lastname: Ming
authors.affiliation: Anhui Luan Blood Center, Luan, Anhui, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Xu
authors.affiliation: Guangdong Guangzhou Blood Center, Guangzhou, Guangdong, China.
authors.forename: Chuanxi
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, Sichuan, China.|Bloodworks Northwest (formerly Puget Sound Blood Center), Seattle, Washington.
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, Sichuan, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Liu
mesh_terms: D015415:Biomarkers / Q000097:blood; D001782:Blood Donors; D058731:Blood Safety / Q000379:methods*; D002681:China; D046148:Donor Selection / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D005500:Follow-Up Studies; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis*; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans
chemical_list: D015415:Biomarkers; D015483:HIV Antibodies