title: Non contiguous-finished genome sequence and description of Bacillus jeddahensis sp. nov.
abstract: Strain JCE(T) was isolated from the fecal sample of a 24-year-old obese man living in Jeddah, Saudi Arabia. It is an aerobic, Gram-positive, rod-shaped bacterium. This strain exhibits a 16S rRNA nucleotide sequence similarity of 97.5 % with Bacillus niacini, the phylogenetically closest species with standing nomenclature. Moreover, the strain JCE(T) presents many phenotypic differences, when it is compared to other Bacillus species, and shows a low MALDI-TOF Mass Spectrometry score that does not allow any identification. Thus, it is likely that this strain represents a new species. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,762,944 bp long genome (1 chromosome but no plasmid) contains 4,654 protein-coding and 98 RNAs genes, including 92 tRNA genes. The strain JCE(T) differs from most of the other closely Bacillus species by more than 1 % in G + C content. In addition, digital DNA-DNA hybridization values for the genome of the strain JCE(T) against the closest Bacillus genomes range between 19.5 to 28.1, that confirming again its new species status. On the basis of these polyphasic data made of phenotypic and genomic analyses, we propose the creation of Bacillus jeddahensis sp. nov. that contains the strain JCE(T).
authors.affiliation: URMITE, Aix-Marseille Université, Faculté de médecine, Marseille, France.
authors.forename: Fadi
authors.initials: F
authors.lastname: Bittar
authors.affiliation: Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Fehmida
authors.initials: F
authors.lastname: Bibi
authors.affiliation: URMITE, Aix-Marseille Université, Faculté de médecine, Marseille, France.
authors.forename: Dhamodharan
authors.initials: D
authors.lastname: Ramasamy
authors.affiliation: URMITE, Aix-Marseille Université, Faculté de médecine, Marseille, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Lagier
authors.affiliation: Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia ; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Esam I
authors.initials: EI
authors.lastname: Azhar
authors.affiliation: Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Asif A
authors.initials: AA
authors.lastname: Jiman-Fatani
authors.affiliation: Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Ahmed K
authors.initials: AK
authors.lastname: Al-Ghamdi
authors.affiliation: URMITE, Aix-Marseille Université, Faculté de médecine, Marseille, France.
authors.forename: Ti Thien
authors.initials: TT
authors.lastname: Nguyen
authors.affiliation: Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Yasir
authors.affiliation: URMITE, Aix-Marseille Université, Faculté de médecine, Marseille, France.
authors.forename: Pierre-Edouard
authors.initials: PE
authors.lastname: Fournier
authors.affiliation: URMITE, Aix-Marseille Université, Faculté de médecine, Marseille, France ; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
keywords: Bacillus jeddahensis; Culturomics; Genome; Human feces; Taxono-genomics