title: Mannan-Binding Lectin Is Involved in the Protection against Renal Ischemia/Reperfusion Injury by Dietary Restriction.
abstract: Preoperative fasting and dietary restriction offer robust protection against renal ischemia/reperfusion injury (I/RI) in mice. We recently showed that Mannan-binding lectin (MBL), the initiator of the lectin pathway of complement activation, plays a pivotal role in renal I/RI. Based on these findings, we investigated the effect of short-term DR (30% reduction of total food intake) or three days of water only fasting on MBL in 10-12 weeks old male C57/Bl6 mice. Both dietary regimens significantly reduce the circulating levels of MBL as well as its mRNA expression in liver, the sole production site of MBL. Reconstitution of MBL abolished the protection afforded by dietary restriction, whereas in the fasting group the protection persisted. These data show that modulation of MBL is involved in the protection against renal I/RI induced by dietary restriction, and suggest that the mechanisms of protection induced by dietary restriction and fasting may be different.
authors.affiliation: Department of Surgery, division of Transplant Surgery, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
authors.forename: Shushimita
authors.initials: S
authors.lastname: Shushimita
authors.affiliation: Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Pieter
authors.initials: P
authors.lastname: van der Pol
authors.affiliation: Department of Surgery, division of Transplant Surgery, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
authors.forename: Ron
authors.initials: R
authors.lastname: W F de Bruin
authors.affiliation: Department of Surgery, division of Transplant Surgery, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
authors.forename: Jan
authors.initials: J
authors.lastname: N M Ijzermans
authors.affiliation: Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Cees
authors.initials: C
authors.lastname: van Kooten
authors.affiliation: Department of Surgery, division of Transplant Surgery, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
authors.forename: Frank J M F
authors.initials: FJ
authors.lastname: Dor
mesh_terms: D000818:Animals; D031204:Caloric Restriction; D005786:Gene Expression Regulation; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D037601:Mannose-Binding Lectin / Q000096:biosynthesis*; D051379:Mice; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*
chemical_list: D037601:Mannose-Binding Lectin