title: Effects of Tempol on Experimental Acute Necrotizing Pancreatitis Model in Rats.
abstract: OBJECTIVE
  We aimed to investigate the effects of Tempol on local organ damage in an experimental acute pancreatitis model.
  
  
  METHODS
  This experimental study was conducted on 40 male Wistar- albino rats. The animals were randomly allocated into four groups: (i) Sham-operated group, laparotomies and cannulations of the pancreatic duct without acute necrotizing pancreatitis (ANP) (n=10); (ii) Sham + Tempol group, identical to group 1 except for intravenous tempol treatment for 4 hours (n = 10); (iii) ANP group, glycodeoxycholic acid was infused into the pancreatic duct and cerulein was infused intravenously for 6 hours for development of ANP (n=10); and (iv) ANP + Tempol treated group, in addition to the procedure in group 3, rats were administered tempol intravenously for 4 hours (n = 10). Injury of the pancreas was evaluated histopathologically. Malondialdehyde and myeloperoxidase levels of the pancreatic tissue, blood gas analysis, leukocyte and hematocrit levels were measured. Wet/dry weight of pancreatic tissue was also measured.
  
  
  RESULTS
  Serum amylase levels, pancreatic tissue malondialdehyde and myeloperoxidase levels, wet/dry weight ratio, pancreatic edema, acinar necrosis, fat necrosis and hemorrhage, inflammation and perivascular infiltration were significantly lower in the ANP + Tempol group compared with the ANP group.
  
  
  CONCLUSIONS
  Tempol infusion reduced local organ damage due to acute necrotizing pancreatitis in this experimental study. These findings demonstrate that tempol has protective effects on local organ damage due to acute necrotizing pancreatitis in rats.
authors.affiliation: a Department of General Surgery, Faculty of Medicine, Akdeniz University , Konyaaltı Antalya , Turkey.
authors.forename: Halil
authors.initials: H
authors.lastname: Erbıs
authors.affiliation: b Department of General Surgery, Faculty of Medicine, Pamukkale University , Kınıklı- Denizli , Turkey.
authors.forename: Muhammed Rasid
authors.initials: MR
authors.lastname: Aykota
authors.affiliation: c Department of Anesthegiology, Faculty of Medicine, Akdeniz University , Konyaaltı Antalya , Turkey.
authors.forename: Bunyamin
authors.initials: B
authors.lastname: Ozturk
authors.affiliation: c Department of Anesthegiology, Faculty of Medicine, Akdeniz University , Konyaaltı Antalya , Turkey.
authors.forename: Burhan
authors.initials: B
authors.lastname: Kabay
authors.affiliation: b Department of General Surgery, Faculty of Medicine, Pamukkale University , Kınıklı- Denizli , Turkey.
authors.forename: Ugur
authors.initials: U
authors.lastname: Sungurtekin
authors.affiliation: b Department of General Surgery, Faculty of Medicine, Pamukkale University , Kınıklı- Denizli , Turkey.
authors.forename: Akın
authors.initials: A
authors.lastname: Ozden
authors.affiliation: d Department of Biochemistry, Faculty of Medicine, Adnan Menderes University , Aydın , Turkey.
authors.forename: Cigdem
authors.initials: C
authors.lastname: Yenisey
authors.affiliation: e Department of Pathology, Faculty of Medicine, Pamukkale University , Kınıklı- Denizli , Turkey.
authors.forename: Nilay Sen
authors.initials: NS
authors.lastname: Turk
authors.affiliation: b Department of General Surgery, Faculty of Medicine, Pamukkale University , Kınıklı- Denizli , Turkey.
authors.forename: Ergun
authors.initials: E
authors.lastname: Erdem
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D003497:Cyclic N-Oxides / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D004487:Edema / Q000209:etiology / Q000517:prevention & control; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009102:Multiple Organ Failure / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019283:Pancreatitis, Acute Necrotizing / Q000097:blood / Q000150:complications* / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D011897:Random Allocation; D017208:Rats, Wistar; D013113:Spin Labels
chemical_list: D000975:Antioxidants; D003497:Cyclic N-Oxides; D013113:Spin Labels; D008315:Malondialdehyde; D009195:Peroxidase; C001803:tempol
keywords: acute necrotizing pancreatitis; free oxygen radical scavenger; tempol