title: RNAi-mediated gene silencing of WsSGTL1 in W.somnifera affects growth and glycosylation pattern.
abstract: Sterol glycosyltransferases (SGTs) belong to family 1 of glycosyltransferases (GTs) and are enzymes responsible for synthesis of sterol-glucosides (SGs) in many organisms. WsSGTL1 is a SGT of Withania somnifera that has been found associated with plasma membranes. However its biological function in W.somnifera is largely unknown. In the present study, we have demonstrated through RNAi silencing of WsSGTL1 gene that it performs glycosylation of withanolides and sterols resulting in glycowithanolides and glycosylated sterols respectively, and affects the growth and development of transgenic W.somnifera. For this, RNAi construct (pFGC1008-WsSGTL1) was made and genetic transformation was done by Agrobacterium tumefaciens. HPLC analysis depicts the reduction of withanoside V (the glycowithanolide of W.somnifera) and a large increase of withanolides (majorly withaferin A) content. Also, a significant decrease in level of glycosylated sterols has been observed. Hence, the obtained data provides an insight into the biological function of WsSGTL1 gene in W.somnifera.
authors.affiliation: a Council of Scientific and Industrial Research - National Botanical Research Institute ; Lucknow ; Uttar Pradesh , India.|b Department of Bioscience ; Integral University ; Lucknow , Uttar Pradesh , India.
authors.forename: Syed
authors.initials: S
authors.lastname: Saema
authors.affiliation: c Council of Scientific and Industrial Research - Central Institute of Medicinal and Aromatic Plants ; Lucknow , Uttar Pradesh , India.
authors.forename: Laiq
authors.initials: L
authors.lastname: ur Rahman
authors.affiliation: a Council of Scientific and Industrial Research - National Botanical Research Institute ; Lucknow ; Uttar Pradesh , India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Niranjan
authors.affiliation: b Department of Bioscience ; Integral University ; Lucknow , Uttar Pradesh , India.
authors.forename: Iffat Zareen
authors.initials: IZ
authors.lastname: Ahmad
authors.affiliation: a Council of Scientific and Industrial Research - National Botanical Research Institute ; Lucknow ; Uttar Pradesh , India.
authors.forename: Pratibha
authors.initials: P
authors.lastname: Misra
mesh_terms: D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005822:Genetic Vectors / Q000378:metabolism; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D034622:RNA Interference; D012038:Regeneration; D013261:Sterols / Q000096:biosynthesis; D014170:Transformation, Genetic; D032341:Withania / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D054358:Withanolides / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D013261:Sterols; D054358:Withanolides; D016695:Glycosyltransferases
keywords: Agrobacterium tumefaciens; Glycosylation; RNAi; Sterols; Transgenic; WsSGTL1