title: Calcium neuroimaging in behaving zebrafish larvae using a turn-key light field camera.
abstract: Reconstructing a three-dimensional scene from multiple simultaneously acquired perspectives (the light field) is an elegant scanless imaging concept that can exceed the temporal resolution of currently available scanning-based imaging methods for capturing fast cellular processes. We tested the performance of commercially available light field cameras on a fluorescent microscopy setup for monitoring calcium activity in the brain of awake and behaving reporter zebrafish larvae. The plenoptic imaging system could volumetrically resolve diverse neuronal response profiles throughout the zebrafish brain upon stimulation with an aversive odorant. Behavioral responses of the reporter fish could be captured simultaneously together with depth-resolved neuronal activity. Overall, our assessment showed that with some optimizations for fluorescence microscopy applications, commercial light field cameras have the potential of becoming an attractive alternative to custom-built systems to accelerate molecular imaging research on cellular dynamics.
authors.affiliation: Helmholtz Zentrum München, Institute of Biological and Medical Imaging, Ingolstädter Landstrasse 1, 85764 Neuherberg, GermanybHelmholtz Zentrum München, Institute of Developmental Genetics, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
authors.forename: Carlos Cruz
authors.initials: CC
authors.lastname: Perez
authors.affiliation: Helmholtz Zentrum München, Institute of Biological and Medical Imaging, Ingolstädter Landstrasse 1, 85764 Neuherberg, GermanybHelmholtz Zentrum München, Institute of Developmental Genetics, Ingolstädter Landstrasse 1, 85764 Neuherberg, GermanycTechnische.
authors.forename: Antonella
authors.initials: A
authors.lastname: Lauri
authors.affiliation: Helmholtz Zentrum München, Institute of Biological and Medical Imaging, Ingolstädter Landstrasse 1, 85764 Neuherberg, GermanybHelmholtz Zentrum München, Institute of Developmental Genetics, Ingolstädter Landstrasse 1, 85764 Neuherberg, GermanycTechnische.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Symvoulidis
authors.affiliation: Helmholtz Zentrum München, Institute of Biological and Medical Imaging, Ingolstädter Landstrasse 1, 85764 Neuherberg, GermanybHelmholtz Zentrum München, Institute of Developmental Genetics, Ingolstädter Landstrasse 1, 85764 Neuherberg, GermanycTechnische.
authors.forename: Michele
authors.initials: M
authors.lastname: Cappetta
authors.affiliation: Raytrix, Schauenburgerstrasse 116, 24118 Kiel, Germany.
authors.forename: Arne
authors.initials: A
authors.lastname: Erdmann
authors.affiliation: Helmholtz Zentrum München, Institute of Biological and Medical Imaging, Ingolstädter Landstrasse 1, 85764 Neuherberg, GermanybHelmholtz Zentrum München, Institute of Developmental Genetics, Ingolstädter Landstrasse 1, 85764 Neuherberg, GermanycTechnische.
authors.forename: Gil Gregor
authors.initials: GG
authors.lastname: Westmeyer
mesh_terms: D000818:Animals; D001923:Brain Chemistry / Q000502:physiology; D002118:Calcium / Q000737:chemistry / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D007814:Larva / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods; D059906:Neuroimaging / Q000379:methods*; D009830:Olfactory Bulb / Q000502:physiology; D015027:Zebrafish
chemical_list: D002118:Calcium