title: Characteristics of Human Turbinate-Derived Mesenchymal Stem Cells Are Not Affected by Allergic Condition of Donor.
abstract: The characteristics of mesenchymal stem cells (MSCs) derived from human turbinates (hTMSCs) have not been investigated in allergic rhinitis. We evaluated the influence of allergic state of the donor on the characteristics, proliferation, and differentiation potential of hTMSCs, compared with hTMSCs derived from non-allergic patients. hTMSCs were isolated from five non-allergic and five allergic patients. The expression of toll-like receptors (TLRs) in hTMSCs was measured by FACS, and cell proliferation was measured using a cell counting kit. Cytokine secretion was analyzed using multiplex immunoassays. The osteogenic, chondrogenic, and adipogenic differentiation potentials of hTMSCs were evaluated by histology and gene expression analysis. In allergic patients, FACS analysis showed that TLR3 and TLR4 were more highly expressed on the surface of hTMSCs than TLR2 and TLR5. The proliferation of hTMSCs was not influenced by the presence of TLR priming. The expression of IL-6, IL-8, IL-12, IP-10, and RANTES was upregulated after the TLR4 priming. The differentiation potential of hTMSCs was not influenced by TLR priming. These characteristics of hTMSCs were similar to those of hTMSCs from non-allergic patients. We conclude that the allergic condition of the donor does not influence TLR expression, proliferation, or immunomodulatory potential of hTMSCs.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea.
authors.forename: Se Hwan
authors.initials: SH
authors.lastname: Hwang
authors.affiliation: Department of Pediatrics, Graduate School of Medicine, Gachon University, Incheon, Korea.
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Cho
authors.affiliation: Institute of Clinical Medicine Research, College of Medicine, Catholic University of Korea, Seoul, Korea.
authors.forename: Sang Hi
authors.initials: SH
authors.lastname: Park
authors.affiliation: Institute of Clinical Medicine Research, College of Medicine, Catholic University of Korea, Seoul, Korea.
authors.forename: WeonSun
authors.initials: W
authors.lastname: Lee
authors.affiliation: Institute of Clinical Medicine Research, College of Medicine, Catholic University of Korea, Seoul, Korea.
authors.forename: Hee Jin
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea.
authors.forename: Dong Chang
authors.initials: DC
authors.lastname: Lee
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea; Department of biomedical science, The Catholic University of Korea, College of Medicine, Seoul, Korea.
authors.forename: Sun Hwa
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea; Department of biomedical science, The Catholic University of Korea, College of Medicine, Seoul, Korea.
authors.forename: Mi Hyun
authors.initials: MH
authors.lastname: Lim
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea.
authors.forename: Sang A
authors.initials: SA
authors.lastname: Back
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea; Department of biomedical science, The Catholic University of Korea, College of Medicine, Seoul, Korea.
authors.forename: Byeong Gon
authors.initials: BG
authors.lastname: Yun
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea.
authors.forename: Dong Il
authors.initials: DI
authors.lastname: Sun
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea.
authors.forename: Jun Myung
authors.initials: JM
authors.lastname: Kang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea.
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000485:Allergens / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D065631:Rhinitis, Allergic / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D012222:Rhinitis, Atrophic / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism; D014420:Turbinates / Q000276:immunology / Q000378:metabolism / Q000473:pathology*
chemical_list: D000485:Allergens; D000954:Antigens, Surface; D016207:Cytokines; D012333:RNA, Messenger; D051193:Toll-Like Receptors