title: Bovine CCL28 Mediates Chemotaxis via CCR10 and Demonstrates Direct Antimicrobial Activity against Mastitis Causing Bacteria.
abstract: In addition to the well characterized function of chemokines in mediating the homing and accumulation of leukocytes to tissues, some chemokines also exhibit potent antimicrobial activity. Little is known of the potential role of chemokines in bovine mammary gland health and disease. The chemokine CCL28 has previously been shown to play a key role in the homing and accumulation of IgA antibody secreting cells to the lactating murine mammary gland. CCL28 has also been shown to act as an antimicrobial peptide with activity demonstrated against a wide range of pathogens including bacteria, fungi and protozoans. Here we describe the cloning and function of bovine CCL28 and document the concentration of this chemokine in bovine milk. Bovine CCL28 was shown to mediate cellular chemotaxis via the CCR10 chemokine receptor and exhibited antimicrobial activity against a variety of bovine mastitis causing organisms. The concentration of bovine CCL28 in milk was found to be highly correlated with the lactation cycle. Highest concentrations of CCL28 were observed soon after parturition, with levels decreasing over time. These results suggest a potential role for CCL28 in the prevention/resolution of bovine mastitis.
authors.affiliation: Department of Immunology and Infectious Diseases, Montana State University, Bozeman, Montana, United States of America.
authors.forename: Kyler B
authors.initials: KB
authors.lastname: Pallister
authors.affiliation: Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, United States of America.
authors.forename: Sara
authors.initials: S
authors.lastname: Mason
authors.affiliation: Department of Immunology and Infectious Diseases, Montana State University, Bozeman, Montana, United States of America.
authors.forename: Tyler K
authors.initials: TK
authors.lastname: Nygaard
authors.affiliation: Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, United States of America.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Immunology and Infectious Diseases, Montana State University, Bozeman, Montana, United States of America.
authors.forename: Shannon
authors.initials: S
authors.lastname: Griffith
authors.affiliation: Department of Immunology and Infectious Diseases, Montana State University, Bozeman, Montana, United States of America.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Jones
authors.affiliation: Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, United States of America.
authors.forename: Susanne
authors.initials: S
authors.lastname: Linderman
authors.affiliation: Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, United States of America.
authors.forename: Melissa
authors.initials: M
authors.lastname: Hughes
authors.affiliation: Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, United States of America.
authors.forename: David
authors.initials: D
authors.lastname: Erickson
authors.affiliation: Department of Immunology and Infectious Diseases, Montana State University, Bozeman, Montana, United States of America.
authors.forename: Jovanka M
authors.initials: JM
authors.lastname: Voyich
authors.affiliation: Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, United States of America.
authors.forename: Mary F
authors.initials: MF
authors.lastname: Davis
authors.affiliation: Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, United States of America.
authors.forename: Eric
authors.initials: E
authors.lastname: Wilson
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D019556:COS Cells; D002417:Cattle; D019742:Chemokines, CC / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D002633:Chemotaxis; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D005260:Female; D005786:Gene Expression Regulation; D008414:Mastitis, Bovine / Q000276:immunology / Q000382:microbiology*; D008892:Milk / Q000276:immunology*; D054447:Receptors, CCR10 / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D019742:Chemokines, CC; D054447:Receptors, CCR10