title: Migration of encephalitogenic CD8 T cells into the central nervous system is dependent on the α4β1-integrin.
abstract: Although CD8 T cells are key players in neuroinflammation, little is known about their trafficking cues into the central nervous system (CNS). We used a murine model of CNS autoimmunity to define the molecules involved in cytotoxic CD8 T-cell migration into the CNS. Using a panel of mAbs, we here show that the α4β1-integrin is essential for CD8 T-cell interaction with CNS endothelium. We also investigated which α4β1-integrin ligands expressed by endothelial cells are implicated. The blockade of VCAM-1 did not protect against autoimmune encephalomyelitis, and only partly decreased the CD8(+) T-cell infiltration into the CNS. In addition, inhibition of junctional adhesion molecule-B expressed by CNS endothelial cells also decreases CD8 T-cell infiltration. CD8 T cells may use additional and possibly unidentified adhesion molecules to gain access to the CNS.
authors.affiliation: Department of Infectious and Tropical Diseases, Toulouse University Hospital, Toulouse, France.|INSERM U1043 - CNRS UMR 5282, Centre de Physiopathologie de Toulouse Purpan (CPTP), Toulouse, France.|Université Toulouse III, Toulouse, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Martin-Blondel
authors.affiliation: INSERM U1043 - CNRS UMR 5282, Centre de Physiopathologie de Toulouse Purpan (CPTP), Toulouse, France.|Université Toulouse III, Toulouse, France.|Department of Clinical Neurosciences, Toulouse University Hospital, Toulouse, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Pignolet
authors.affiliation: Theodor Kocher Institute, University of Bern, Bern, Switzerland.
authors.forename: Silvia
authors.initials: S
authors.lastname: Tietz
authors.affiliation: INSERM U1043 - CNRS UMR 5282, Centre de Physiopathologie de Toulouse Purpan (CPTP), Toulouse, France.|Université Toulouse III, Toulouse, France.
authors.forename: Lidia
authors.initials: L
authors.lastname: Yshii
authors.affiliation: INSERM U1043 - CNRS UMR 5282, Centre de Physiopathologie de Toulouse Purpan (CPTP), Toulouse, France.|Université Toulouse III, Toulouse, France.
authors.forename: Christina
authors.initials: C
authors.lastname: Gebauer
authors.affiliation: Theodor Kocher Institute, University of Bern, Bern, Switzerland.
authors.forename: Therese
authors.initials: T
authors.lastname: Perinat
authors.affiliation: INSERM U1043 - CNRS UMR 5282, Centre de Physiopathologie de Toulouse Purpan (CPTP), Toulouse, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Van Weddingen
authors.affiliation: Theodor Kocher Institute, University of Bern, Bern, Switzerland.
authors.forename: Claudia
authors.initials: C
authors.lastname: Blatti
authors.affiliation: Theodor Kocher Institute, University of Bern, Bern, Switzerland.
authors.forename: Britta
authors.initials: B
authors.lastname: Engelhardt
authors.affiliation: INSERM U1043 - CNRS UMR 5282, Centre de Physiopathologie de Toulouse Purpan (CPTP), Toulouse, France.|Université Toulouse III, Toulouse, France.
authors.forename: Roland
authors.initials: R
authors.lastname: Liblau
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000502:physiology; D002465:Cell Movement; D004679:Encephalomyelitis / Q000209:etiology*; D039041:Integrin alpha4beta1 / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000502:physiology; D019010:Vascular Cell Adhesion Molecule-1 / Q000502:physiology; D062765:Junctional Adhesion Molecule B
chemical_list: D015815:Cell Adhesion Molecules; D039041:Integrin alpha4beta1; D019010:Vascular Cell Adhesion Molecule-1; C498781:Jam2 protein, mouse; D062765:Junctional Adhesion Molecule B
keywords: CD8 T cell; Junctional adhesion molecule-B; Migration; Neuroinflammation; α4β1-Integrin