title: [Evaluation of the effect for ffh gene silencing on the aciduricity of fluoride resistant Streptococcus mutans].
abstract: OBJECTIVE
  To analyze the effect of ffh gene silencing on the aciduricity of fluoride resistant Streptococcus mutans in vitro.
  
  
  METHODS
  By using electroporation, UA159-FR was transformed and combined with targeted site of ffh gene sequence, and the best piece of siRNA for fluoride resistant Streptococcus mutans was screened. In different values of pH of BHI, they were cultured for 24 hours with UA159-FR respectively, and then centrifugated to determine the pH and OD600. SPSS17.0 software package was used to analyze the data.
  
  
  RESULTS
  The aciduricity of UA159-FR had significant differences compared with ffh gene silencing for UA159-FR in δpH (P<0.05), and the former was higher than the latter. At pH=3.5-5.0, P<0.01; at pH=5.5-7.5, P<0.05. Significant differences were noted in OD600 and their growth tendency were similar.
  
  
  CONCLUSIONS
  The aciduricity of fluoride resistant Streptococcus mutans has significant effect when the ffh gene is silenced.
authors.affiliation: Department of Endodontics, Hospital of Stomatology, Jilin University. Changchun 130021, Jilin Province, China.Lizhenling0722@126.com.
authors.forename: Zhen-ling
authors.initials: ZL
authors.lastname: Li
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: Zhang
authors.forename: Peng-lian
authors.initials: PL
authors.lastname: Li
authors.forename: Tong-fei
authors.initials: TF
authors.lastname: Zhang
authors.forename: Tian-bo
authors.initials: TB
authors.lastname: Li
authors.forename: Fu-ping
authors.initials: FP
authors.lastname: Liu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002327:Cariostatic Agents / Q000494:pharmacology*; D005459:Fluorides / Q000494:pharmacology*; D020868:Gene Silencing; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010710:Phosphates; D013295:Streptococcus mutans / Q000187:drug effects*
chemical_list: D001426:Bacterial Proteins; D002327:Cariostatic Agents; D010710:Phosphates; C012980:fluorophosphate; D005459:Fluorides