title: Mipu1 inhibits lipid accumulation through down-regulation of CD36 in RAW264.7 cells.
abstract: OBJECTIVE
  Our recent data indicated that Mipu1 overexpression reduces lipid intake and CD36 expression of macrophages in the presence of oxLDL. However, the mechanism of Mipu1 inhibiting lipid accumulation in macrophages is not elucidated.
  
  
  METHODS
  Real-time quantitative polymerase chain reaction (PCR) and western blot analysis were used to detect expression of Mipu1 and CD36. The promoter activity of CD36 was studied using luciferase assays. Chromatin immunoprecipitation (ChIP) was used to show the recruitment of Mipu1 onto the CD36 promoter. High-performance liquid chromatography and Dil-labeled lipoprotein were used to detect cholesterol accumulation.
  
  
  RESULTS
  Here, we show that CD36 overexpression rescues oxLDL-induced cholesterol accumulation in RAW264.7-Mipu1 cells. Analysis of the mouse CD36 promoter revealed two potential Mipu1-response elements (MRE), one of which (from -237bp to -244bp, ACTTAC) was shown, using mutagenesis and deletion analysis, to be functional. Mipu1 was demonstrated to bind to CD36 promoter, and oxLDL treatment resulted in increases in their interaction as assessed by ChIP.
  
  
  CONCLUSIONS
  It was demonstrated that Mipu1 inhibited the lipid accumulation of macrophages and it down-regulated CD36 expression in the presence of oxLDL.
authors.affiliation: Post-doctoral Mobile Stations for Basic Medicine, Institute of Cardiovascular Disease and Key Lab for Arteriosclerology of Hunan Province, University of South China, Hengyang City, China.
authors.forename: Shun-Lin
authors.initials: SL
authors.lastname: Qu
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Fan
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Guo
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Pan
authors.forename: Dan
authors.initials: D
authors.lastname: Han
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yu-Ning
authors.initials: YN
authors.lastname: Zhu
authors.forename: Zhi-Sheng
authors.initials: ZS
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001665:Binding Sites; D018955:CD36 Antigens / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000494:pharmacology; D051379:Mice; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D000067996:RAW 264.7 Cells
chemical_list: D018955:CD36 Antigens; D004268:DNA-Binding Proteins; D008077:Lipoproteins, LDL; C000609567:Mipu1 protein, mouse; D009687:Nuclear Proteins; C099252:oxidized low density lipoprotein; D002784:Cholesterol