title: Encephalomyocarditis Virus 3C Protease Relieves TRAF Family Member-associated NF-κB Activator (TANK) Inhibitory Effect on TRAF6-mediated NF-κB Signaling through Cleavage of TANK.
abstract: TRAF family member-associated NF-κB activator (TANK) is a negative regulator of canonical NF-κB signaling in the Toll-like receptor- and B-cell receptor-mediated signaling pathways. However, functions of TANK in viral infection-mediated NF-κB activation remain unclear. Here, we reported that TANK was cleaved by encephalomyocarditis virus 3C at the 197 and 291 glutamine residues, which depends on its cysteine protease activity. In addition, encephalomyocarditis virus 3C impaired the ability of TANK to inhibit TRAF6-mediated NF-κB signaling. Interestingly, we found that several viral proteases encoded by the foot and mouth disease virus, porcine reproductive and respiratory syndrome virus, and equine arteritis virus also cleaved TANK. Our results suggest that TANK is a novel target of some viral proteases, indicating that some positive RNA viruses have evolved to utilize their major proteases to regulate NF-κB activation.
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001.
authors.forename: Qinfang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001.
authors.forename: Lijie
authors.initials: L
authors.lastname: Zhang
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, the College of Life Sciences, Yangtze University, Jingzhou 434100.
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001.
authors.forename: Liang
authors.initials: L
authors.lastname: Hu
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001.
authors.forename: Changyao
authors.initials: C
authors.lastname: Li
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Wang
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001.
authors.forename: Jiangnan
authors.initials: J
authors.lastname: Li
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001.
authors.forename: Yuanfeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001.
authors.forename: Huibin
authors.initials: H
authors.lastname: Yu
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, the Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650093, and.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: the Department of Microbiology, Harbin Medical University, Harbin 150081, China.
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: the College of Life Sciences, Yangtze University, Jingzhou 434100.
authors.forename: Tao
authors.initials: T
authors.lastname: Xiong
authors.affiliation: the Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650093, and.
authors.forename: Xueshan
authors.initials: X
authors.lastname: Xia
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Wang
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001.
authors.forename: Guohua
authors.initials: G
authors.lastname: Deng
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Cai
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, cuishangjin@caas.cn.
authors.forename: Shangjin
authors.initials: S
authors.lastname: Cui
authors.affiliation: From the State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, wengcj@hvri.ac.cn.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Weng
mesh_terms: D000086042:3C Viral Proteases; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000595:Amino Acid Sequence; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D004686:Encephalomyocarditis virus / Q000201:enzymology*; D007240:Equartevirus / Q000201:enzymology; D029544:Foot-and-Mouth Disease Virus / Q000201:enzymology; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism*; D019316:Porcine respiratory and reproductive syndrome virus / Q000201:enzymology; D059748:Proteolysis; D048029:TNF Receptor-Associated Factor 6 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D016328:NF-kappa B; C484406:TANK protein, human; D048029:TNF Receptor-Associated Factor 6; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases
keywords: NF-kappa B (NF-KB); cell signaling; immunology; viral protease; virus