title: Association between CD14 gene promoter polymorphisms with serum total-IgE and eosinophil levels in atopic and non-atopic asthma patients in a Chinese Han population.
abstract: OBJECTIVE
  The aim of this study was to investigate the association between CD14 gene promoter SNPs with serum total-IgE and eosinophil levels in atopic asthma and non-atopic asthma in Chinese Han.
  
  
  METHODS
  A total of 152 patients with asthma were divided into atopic asthma (n = 100) and non-atopic asthma (n = 52) groups for this study. Six CD14 gene SNPs were analyzed using PCR and gene sequencing. Serum total-IgE and eosinophil levels were measured. The association between genotype frequencies of the CD14 gene loci with total-IgE and eosinophil levels in atopic asthma and non-atopic asthma was evaluated by the ANOVA test method. Hundred and sixteen healthy subjects constitute the control group.
  
  
  RESULTS
  We found that serum total-IgE and eosinophil levels were significantly higher in individuals with atopic asthma when compared to individuals with non-atopic asthma (p < 0.01). For non-atopic asthma, the total-IgE levels of the heterozygous genotypes were significantly higher than the corresponding levels for the homozygous genotypes in CD14-260C > T, CD-651C > T, CD-911A > C and CD-1247A > G (p < 0.01). In atopic asthma, there was no statistical significance for either serum total-IgE or eosinophil levels among the genotypes of the CD14 gene SNPs. In addition, allele A frequency of CD14-1247A > G was significantly different between the atopic asthma and non-atopic asthma groups (p = 0.025).
  
  
  CONCLUSIONS
  There was a statistical association between the serum total-IgE level and the CD14 gene promoter SNPs in the non-atopic asthma group. The eosinophil level was not found to be statistically associated with the CD14 gene promoter SNPs in either the atopic asthma or non-atopic asthma groups.
authors.affiliation: a Clinical Laboratory Medicine, Yan Tai Affiliated Hospital of Binzhou Medical University , Yantai , China .
authors.forename: Jiankai
authors.initials: J
authors.lastname: Feng
authors.affiliation: b Center of Laboratory Medicine, the Affiliated Yuhuangding Hospital of Qingdao University Medical College , Yantai , China , and.
authors.forename: Caiji
authors.initials: C
authors.lastname: Zhang
authors.affiliation: c Yantai Municipal Center for Disease Control and Prevention , Yantai , China.
authors.forename: Zongguo
authors.initials: Z
authors.lastname: Wang
authors.affiliation: b Center of Laboratory Medicine, the Affiliated Yuhuangding Hospital of Qingdao University Medical College , Yantai , China , and.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: b Center of Laboratory Medicine, the Affiliated Yuhuangding Hospital of Qingdao University Medical College , Yantai , China , and.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: b Center of Laboratory Medicine, the Affiliated Yuhuangding Hospital of Qingdao University Medical College , Yantai , China , and.
authors.forename: Hongling
authors.initials: H
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D044466:Asian People / Q000235:genetics; D001249:Asthma / Q000097:blood / Q000235:genetics / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D004804:Eosinophils / Q000276:immunology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000235:genetics / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood*; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D055815:Young Adult
chemical_list: D018950:Lipopolysaccharide Receptors; D007073:Immunoglobulin E
keywords: Atopic asthma; CD14; eosinophil; non-atopic asthma; serum total-IgE; single nucleotide polymorphism