title: Luxury uptake of phosphorus changes the accumulation of starch and lipid in Chlorella sp. under nitrogen depletion.
abstract: The aim of this research was to study the effect of phosphorus supply on starch and lipid production under nitrogen starvation using Chlorella sp. as a model. High phosphate level had marginal effect on cell density but increased biomass growth. Massive phosphorus was assimilated quickly and mainly stored in the form of polyphosphate. The algal cells ceased phosphorus uptake when intracellular phosphorus reached a certain level. 5mM phosphate in the culture rendered a 16.7% decrease of starch synthesis and a 22.4% increase of lipid synthesis relative to low phosphate (0.17 mM). It is plausible that phosphate can regulate carbon partitioning between starch and lipid synthesis pathway by influencing ADP-glucose pyrophosphorylase activity. Moreover, high phosphate concentration enhanced the abundance of oleic acid, improving oil quality for biodiesel production. It is a promising cultivation strategy by integration of phosphorus removal from wastewater with biodiesel production for this alga.
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Shunni
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Changhua
authors.initials: C
authors.lastname: Shang
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Jingliang
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address: yuanzh@ms.giec.ac.cn.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D056804:Biofuels; D018533:Biomass; D002708:Chlorella / Q000378:metabolism*; D008055:Lipids; D009584:Nitrogen / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D011122:Polyphosphates / Q000378:metabolism; D013213:Starch / Q000378:metabolism*; D062065:Wastewater
chemical_list: D056804:Biofuels; D008055:Lipids; D009584:Nitrogen; D010758:Phosphorus; D011122:Polyphosphates; D013213:Starch; D062065:Wastewater
keywords: Chlorella sp.; Fatty acids; Nitrogen depletion; Phosphorus uptake; Starch