title: Aptamer loaded MoS2 nanoplates as nanoprobes for detection of intracellular ATP and controllable photodynamic therapy.
abstract: In this work we designed a MoS2 nanoplate-based nanoprobe for fluorescence imaging of intracellular ATP and photodynamic therapy (PDT) via ATP-mediated controllable release of (1)O2. The nanoprobe was prepared by simply assembling a chlorine e6 (Ce6) labelled ATP aptamer on MoS2 nanoplates, which have favorable biocompatibility, unusual surface-area-to-mass ratio, strong affinity to single-stranded DNA, and can quench the fluorescence of Ce6. After the nanoprobe was internalized into the cells and entered ATP-abundant lysosomes, its recognition to ATP led to the release of the single-stranded aptamer from MoS2 nanoplates and thus recovered the fluorescence of Ce6 at an excitation wavelength of 633 nm, which produced a highly sensitive and selective method for imaging of intracellular ATP. Meanwhile, the ATP-mediated release led to the generation of (1)O2 under 660 nm laser irradiation, which could induce tumor cell death with a lysosomal pathway. The controllable PDT provided a model approach for design of multifunctional theranostic nanoprobes. These results also promoted the development and application of MoS2 nanoplate-based platforms in biomedicine.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China. hxju@nju.edu.cn jsyu@nju.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Jia
authors.forename: Lin
authors.initials: L
authors.lastname: Ding
authors.forename: Jiangwei
authors.initials: J
authors.lastname: Tian
authors.forename: Lei
authors.initials: L
authors.lastname: Bao
authors.forename: Yaoping
authors.initials: Y
authors.lastname: Hu
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
authors.forename: Jun-Sheng
authors.initials: JS
authors.lastname: Yu
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D004220:Disulfides / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D057054:Molecular Imaging; D015335:Molecular Probes / Q000737:chemistry*; D008982:Molybdenum / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D010778:Photochemotherapy
chemical_list: D004220:Disulfides; D015335:Molecular Probes; D008982:Molybdenum; D000255:Adenosine Triphosphate; C082964:molybdenum disulfide