title: Heterosexual Transmission of Subtype C HIV-1 Selects Consensus-Like Variants without Increased Replicative Capacity or Interferon-α Resistance.
abstract: Heterosexual transmission of HIV-1 is characterized by a genetic bottleneck that selects a single viral variant, the transmitted/founder (TF), during most transmission events. To assess viral characteristics influencing HIV-1 transmission, we sequenced 167 near full-length viral genomes and generated 40 infectious molecular clones (IMC) including TF variants and multiple non-transmitted (NT) HIV-1 subtype C variants from six linked heterosexual transmission pairs near the time of transmission. Consensus-like genomes sensitive to donor antibodies were selected for during transmission in these six transmission pairs. However, TF variants did not demonstrate increased viral fitness in terms of particle infectivity or viral replicative capacity in activated peripheral blood mononuclear cells (PBMC) and monocyte-derived dendritic cells (MDDC). In addition, resistance of the TF variant to the antiviral effects of interferon-α (IFN-α) was not significantly different from that of non-transmitted variants from the same transmission pair. Thus neither in vitro viral replicative capacity nor IFN-α resistance discriminated the transmission potential of viruses in the quasispecies of these chronically infected individuals. However, our findings support the hypothesis that within-host evolution of HIV-1 in response to adaptive immune responses reduces viral transmission potential.
authors.affiliation: Emory Vaccine Center at Yerkes National Primate Research Center, Emory University, Atlanta, Georgia, United States of America.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Deymier
authors.affiliation: Emory Vaccine Center at Yerkes National Primate Research Center, Emory University, Atlanta, Georgia, United States of America.
authors.forename: Zachary
authors.initials: Z
authors.lastname: Ende
authors.affiliation: Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
authors.forename: Angharad E
authors.initials: AE
authors.lastname: Fenton-May
authors.affiliation: Emory Vaccine Center at Yerkes National Primate Research Center, Emory University, Atlanta, Georgia, United States of America.
authors.forename: Dario A
authors.initials: DA
authors.lastname: Dilernia
authors.affiliation: Zambia-Emory HIV Research Project, Lusaka, Zambia.
authors.forename: William
authors.initials: W
authors.lastname: Kilembe
authors.affiliation: Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, United States of America.
authors.forename: Susan A
authors.initials: SA
authors.lastname: Allen
authors.affiliation: Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
authors.forename: Persephone
authors.initials: P
authors.lastname: Borrow
authors.affiliation: Emory Vaccine Center at Yerkes National Primate Research Center, Emory University, Atlanta, Georgia, United States of America; Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, United States of America.
authors.forename: Eric
authors.initials: E
authors.lastname: Hunter
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology; D002478:Cells, Cultured; D015331:Cohort Studies; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology / Q000821:virology; D024882:Drug Resistance, Viral; D005191:Family Characteristics; D005260:Female; D014644:Genetic Variation; D015658:HIV Infections / Q000276:immunology / Q000473:pathology / Q000635:transmission* / Q000821:virology; D006679:HIV Seropositivity / Q000276:immunology / Q000473:pathology / Q000635:transmission* / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D020010:Heterosexuality; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000473:pathology / Q000821:virology; D008297:Male; D008969:Molecular Sequence Data; D058889:Molecular Typing; D010802:Phylogeny; D014771:Virion / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D053586:Virus Internalization / Q000187:drug effects; D014779:Virus Replication; D015024:Zambia
chemical_list: D000998:Antiviral Agents; D016898:Interferon-alpha