title: The Effect of Local Delivery Doxycycline and Alendronate on Bone Repair.
abstract: The aim of the present study was to investigate the local effect of 10% doxycycline and 1% alendronate combined with poly(lactic-co-glycolic acid) (PLGA) on bone repair. Thirty rats were divided into three groups, as follows: control group (CG), drug group (DG), and vehicle-PLGA group (VG). Bone defect was created in the right femur and filled with the following: blood clot (CG); PLGA gel, 10% doxycycline and 1% alendronate (DG); or vehicle-PLGA (VG). The animals were euthanized 7 or 15 days after surgery. Bone density, bone matrix and number of osteoclasts were quantified. At 7 days, the findings showed increased density in DG (177.75 ± 76.5) compared with CG (80.37 ± 27.4), but no difference compared with VG (147.1 ± 41.5); no statistical difference in bone neoformation CG (25.6 ± 4.8), VG (27.8 ± 4), and DG (18.9 ± 7.8); and decrease osteoclasts in DG (4.6 ± 1.9) compared with CG (26.7 ± 7.4) and VG (17.3 ± 2.7). At 15 days, DG (405.1 ± 63.1) presented higher density than CG (213.2 ± 60.9) and VG (283.4 ± 85.8); there was a significant increase in percentage of bone neoformation in DG (31.5 ± 4.2) compared with CG (23 ± 4), but no difference compared with VG (25.1 ± 2.9). There was a decreased number of osteoclasts in DG (20.7 ± 4.7) and VG (29.5 ± 5.4) compared with CG (40 ± 9.4). The results suggest that the association of 10% doxycycline and 1% alendronate with PLGA-accelerated bone repair.
authors.affiliation: Integrated Dental Clinic Program, School of Dentistry, University of Uberlândia, Minas Gerais, Brazil. pedro_hjlo@hotmail.com.|Departamento de Cirurgia e Traumatologia Buco-Maxilo-Facial, Bairro Umuarama, Avenida Pará s/n°, Campus Umuarama, Bloco 4T, Uberlândia, Minas Gerais, Brazil, 38.400-902. pedro_hjlo@hotmail.com.
authors.forename: Pedro Henrique Justino Oliveira
authors.initials: PH
authors.lastname: Limirio
authors.affiliation: University of Uberlândia, Minas Gerais, Brazil.
authors.forename: Flaviana Soares
authors.initials: FS
authors.lastname: Rocha
authors.affiliation: University of Uberlândia, Minas Gerais, Brazil.
authors.forename: Jonas Dantas
authors.initials: JD
authors.lastname: Batista
authors.affiliation: University of Uberlândia, Minas Gerais, Brazil.
authors.forename: João César
authors.initials: JC
authors.lastname: Guimarães-Henriques
authors.affiliation: University of Uberlândia, Minas Gerais, Brazil.
authors.forename: Geraldo Batista
authors.initials: GB
authors.lastname: de Melo
authors.affiliation: University of Uberlândia, Minas Gerais, Brazil.
authors.forename: Paula
authors.initials: P
authors.lastname: Dechichi
mesh_terms: D019386:Alendronate / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D050071:Bone Density Conservation Agents / Q000008:administration & dosage / Q000737:chemistry; D001861:Bone Regeneration / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects*; D004318:Doxycycline / Q000008:administration & dosage* / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D019344:Lactic Acid / Q000737:chemistry; D008297:Male; D011091:Polyesters / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017208:Rats, Wistar
chemical_list: D050071:Bone Density Conservation Agents; D011091:Polyesters; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D004318:Doxycycline; D019386:Alendronate
keywords: alendronate; bone repair; doxycycline