title: [Optimization and application of targeted LC-MS metabolomic analyses in photosynthetic cyanobacteria].
abstract: To accurately analyze metabolites in industry-important photosynthetic microbes, LC-MS based metabolomics protocol needs to be optimized specifically for individual species. In this study, an LC-MS based metabolomics method was optimized for cyanobacterium Synechocystis sp. PCC 6803. With the optimized extraction, liquid chromatographic and mass spectral parameters, the method was capable of detecting 24 important metabolites related to central carbohydrate and energy metabolism in Synechocystis sp. PCC 6803. The study laid an important foundation for the metabolomics analysis of cyanobacteria.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Niu
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Zhang
authors.forename: Xinheng
authors.initials: X
authors.lastname: Yu
authors.forename: Yingxue
authors.initials: Y
authors.lastname: Su
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Weiwen
authors.initials: W
authors.lastname: Zhang
mesh_terms: D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D055442:Metabolome; D055432:Metabolomics; D010788:Photosynthesis; D046939:Synechocystis / Q000378:metabolism*