title: HIV Disrupts Human T Cells That Target Mycobacterial Glycolipids.
abstract: Single-cell analysis captures the heterogeneity of T-cell populations that target defined antigens. Human immunodeficiency virus (HIV) infection results in defects of antimycobacterial immunity, which remain poorly defined. We therefore recruited a small number of subjects, including those with latent and active M. tuberculosis infection, with or without concomitant HIV infection, and tracked the mycobacterial glycolipid-reactive T-cell repertoire by using CD1b tetramers. Glycolipid-reactive T cells expressed memory markers and the HIV coreceptors CD4 and CCR5; they were not detected in subjects with HIV-associated active M. tuberculosis infection. HIV infection may affect T cells that recognize mycobacterial glycolipids and influence immunity.
authors.affiliation: KwaZulu-Natal Research Institute for Tuberculosis and HIV, Doris Duke Medical Research Institute, Nelson R. Mandela School of Medicine, University of KwaZulu-Natal Ragon Institute of MGH, MIT, and Harvard, Cambridge.
authors.forename: Victoria O
authors.initials: VO
authors.lastname: Kasprowicz
authors.affiliation: Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.
authors.forename: Tan-Yun
authors.initials: TY
authors.lastname: Cheng
authors.affiliation: KwaZulu-Natal Research Institute for Tuberculosis and HIV, Doris Duke Medical Research Institute, Nelson R. Mandela School of Medicine, University of KwaZulu-Natal HIV Pathogenesis Programme, Doris Duke Medical Research Institute, Nelson R. Mandela School of Medicine, University of KwaZulu-Natal Ragon Institute of MGH, MIT, and Harvard, Cambridge Max Planck Institute for Infection Biology, Berlin, Germany.
authors.forename: Thumbi
authors.initials: T
authors.lastname: Ndung'u
authors.affiliation: Infectious Diseases Unit, Doris Duke Medical Research Institute, Nelson R. Mandela School of Medicine, University of KwaZulu-Natal McCord Hospital, Durban, South Africa.
authors.forename: Henry
authors.initials: H
authors.lastname: Sunpath
authors.affiliation: Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.
authors.forename: D Branch
authors.initials: DB
authors.lastname: Moody
authors.affiliation: Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.
authors.forename: Anne G
authors.initials: AG
authors.lastname: Kasmar
mesh_terms: D000328:Adult; D015704:CD4 Antigens / Q000032:analysis; D060085:Coinfection / Q000276:immunology; D006017:Glycolipids / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D008875:Middle Aged; D009161:Mycobacterium / Q000276:immunology*; D019713:Receptors, CCR5 / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology*
chemical_list: C584933:CCR5 protein, human; D015704:CD4 Antigens; D006017:Glycolipids; D019713:Receptors, CCR5
keywords: CD1; HIV; T cell; antigens; glycolipids; tetramer; tuberculosis