title: Global Identification of MicroRNAs and Their Targets in Barley under Salinity Stress.
abstract: Salinity is a major limiting factor for agricultural production worldwide. A better understanding of the mechanisms of salinity stress response will aid efforts to improve plant salt tolerance. In this study, a combination of small RNA and mRNA degradome sequencing was used to identify salinity responsive-miRNAs and their targets in barley. A total of 152 miRNAs belonging to 126 families were identified, of which 44 were found to be salinity responsive with 30 up-regulated and 25 down-regulated respectively. The majority of the salinity-responsive miRNAs were up-regulated at the 8h time point, while down-regulated at the 3h and 27h time points. The targets of these miRNAs were further detected by degradome sequencing coupled with bioinformatics prediction. Finally, qRT-PCR was used to validate the identified miRNA and their targets. Our study systematically investigated the expression profile of miRNA and their targets in barley during salinity stress phase, which can contribute to understanding how miRNAs respond to salinity stress in barley and other cereal crops.
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Pingchuan
authors.initials: P
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Le
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Licao
authors.initials: L
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Kewei
authors.initials: K
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Fuyan
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Xianghong
authors.initials: X
authors.lastname: Du
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tong
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Wanquan
authors.initials: W
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Song
authors.initials: S
authors.lastname: Weining
mesh_terms: D001483:Base Sequence; D019295:Computational Biology; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D017343:Genes, Plant / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D001467:Hordeum / Q000235:genetics* / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics*; D020871:RNA Stability / Q000235:genetics; D054712:Salinity; D017423:Sequence Analysis, RNA; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D035683:MicroRNAs