title: Application of chrysophanol in zebrafish to reduce dietary introduced lipid and its possible mechanism.
abstract: OBJECTIVE
  To explore the therapeutic potential and mechanism of chrysophanol on lipid-lowering function.
  
  
  METHODS
  Zebrafish or larvae were employed to evaluate the effect of chrysophanol on lipid-lowering. Zebrafish of 5 day post fertilization (dpf, larva) and 13-week old were fed with high-cholesterol diet or high-fat to investigate the influence of chrysophanol comparing with atorvastain and co-administration of chrysophanol and atorvastain on subsistent blood lipid using the fluorescence microscope and lipid panel screen. Thereafter, we enrolled zebrafish of 7 dpf fed with high-fat diet to explore the lipid-lowering mechanism of chrysophanol basing on the frequency of peristalsis and the residual on the digestive wall.
  
  
  RESULTS
  Chrysophanol could significantly lower cholesterol both in zebrafish and larvae (P < 0.05), and the co-administration of chrysophanol and atorvastatin had the best performance in reducing cholesterol (P < 0.05). Chrysophanol and atorvastain could also significantly lower triglyceride. Moreover, we found that chrysophanol attached on the digestive wall for a long time and enhanced the frequency of peristalsis.
  
  
  CONCLUSIONS
  Chrysophanol has lipid-lowering effect both in zebrafish and larvae which may be attributed to the effect on the frequency of peristalsis and fat absorption, and co-administration with atorvastain performs better lipid-lowering effect in zebrafish.
authors.affiliation: Department of Cardiology, Shanghai Ninth People's Hospital Affiliated Shanghai Jiaotong University School of Medicine Shanghai 200011, China.
authors.forename: Kan
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Shanghai Ninth People's Hospital Affiliated Shanghai Jiaotong University School of Medicine Shanghai 200011, China.
authors.forename: Chang-Qian
authors.initials: CQ
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Shanghai Ninth People's Hospital Affiliated Shanghai Jiaotong University School of Medicine Shanghai 200011, China.
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Fan
authors.affiliation: Department of Cardiology, Shanghai Ninth People's Hospital Affiliated Shanghai Jiaotong University School of Medicine Shanghai 200011, China.
authors.forename: Yu-Shui
authors.initials: YS
authors.lastname: Xie
authors.affiliation: Department of Cardiology, Shanghai Ninth People's Hospital Affiliated Shanghai Jiaotong University School of Medicine Shanghai 200011, China.
authors.forename: Zhao-Fang
authors.initials: ZF
authors.lastname: Yin
authors.affiliation: Department of Cardiology, Shanghai Ninth People's Hospital Affiliated Shanghai Jiaotong University School of Medicine Shanghai 200011, China.
authors.forename: Zuo-Jun
authors.initials: ZJ
authors.lastname: Xu
authors.affiliation: Department of Cardiology, Shanghai Ninth People's Hospital Affiliated Shanghai Jiaotong University School of Medicine Shanghai 200011, China.
authors.forename: Hui-Li
authors.initials: HL
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Shanghai Ninth People's Hospital Affiliated Shanghai Jiaotong University School of Medicine Shanghai 200011, China.
authors.forename: Jia-Tian
authors.initials: JT
authors.lastname: Cao
authors.affiliation: Department of Cardiology, Shanghai Ninth People's Hospital Affiliated Shanghai Jiaotong University School of Medicine Shanghai 200011, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
keywords: Chrysophanol; atorvastain; lipid-lowering effect; zebrafish