title: Association of TLR4 polymorphisms with Mycobacterium avium subspecies paratuberculosis infection status in Canadian Holsteins.
abstract: Mycobacterium avium ssp. paratuberculosis (MAP) causes chronic enteritis in cattle that results in substantial financial losses to the cattle industry worldwide. Given that susceptibility to MAP infection is determined in part by genetics, marker-assisted selection may help in the breeding of animals that are more resistant to MAP infection. The toll-like receptor 4 gene (TLR4) was selected as a potential candidate gene because of its role in innate immunity and its involvement in MAP recognition and infection. The objective of this study, therefore, was to identify associations between TLR4 polymorphisms and susceptibility to MAP infection in Canadian Holstein cows. Two biologically relevant SNPs, including c.-226G>C in the 5'-untranslated region and the non-synonymous SNP c.2021C>T in the potential TIR domain, were selected for an association analysis with MAP infection status in 409 Canadian Holsteins. The haplotype C-T from these combined SNPs yielded significant association with susceptibility to MAP infection, supporting the involvement of TLR4 in susceptibility to MAP infection.
authors.affiliation: Animal and Poultry Science, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: B S
authors.initials: BS
authors.lastname: Sharma
authors.affiliation: Animal and Poultry Science, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.|Department of Animal and Poultry Science, Damanhour University, Damanhour, Egypt.
authors.forename: M K
authors.initials: MK
authors.lastname: Abo-Ismail
authors.affiliation: Animal and Poultry Science, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: F S
authors.initials: FS
authors.lastname: Schenkel
authors.affiliation: Animal and Poultry Science, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: Q
authors.initials: Q
authors.lastname: You
authors.affiliation: Pathology and Molecular Medicine, McMaster University, Hamilton, Canada.
authors.forename: C P
authors.initials: CP
authors.lastname: Verschoor
authors.affiliation: Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, Australia.
authors.forename: S D
authors.initials: SD
authors.lastname: Pant
authors.affiliation: Animal and Poultry Science, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: N A
authors.initials: NA
authors.lastname: Karrow
mesh_terms: D000818:Animals; D002170:Canada; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics* / Q000382:microbiology; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D015810:Linkage Disequilibrium; D008957:Models, Genetic; D016927:Mycobacterium avium subsp. paratuberculosis; D010283:Paratuberculosis / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D051197:Toll-Like Receptor 4 / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D051197:Toll-Like Receptor 4
keywords: Johne's disease; SNP; bovine; toll-like receptor 4