title: Booster dose after 10 years is recommended following 17DD-YF primary vaccination.
abstract: A single vaccination of Yellow Fever vaccines is believed to confer life-long protection. In this study, results of vaccinees who received a single dose of 17DD-YF immunization followed over 10 y challenge this premise. YF-neutralizing antibodies, subsets of memory T and B cells as well as cytokine-producing lymphocytes were evaluated in groups of adults before (NVday0) and after (PVday30-45, PVyear1-4, PVyear5-9, PVyear10-11, PVyear12-13) 17DD-YF primary vaccination. YF-neutralizing antibodies decrease significantly from PVyear1-4 to PVyear12-13 as compared to PVday30-45, and the seropositivity rates (PRNT≥2.9Log10mIU/mL) become critical (lower than 90%) beyond PVyear5-9. YF-specific memory phenotypes (effector T-cells and classical B-cells) significantly increase at PVday30-45 as compared to naïve baseline. Moreover, these phenotypes tend to decrease at PVyear10-11 as compared to PVday30-45. Decreasing levels of TNF-α(+) and IFN-γ(+) produced by CD4(+) and CD8(+) T-cells along with increasing levels of IL-10(+)CD4(+)T-cells were characteristic of anti-YF response over time. Systems biology profiling represented by hierarchic networks revealed that while the naïve baseline is characterized by independent micro-nets, primary vaccinees displayed an imbricate network with essential role of central and effector CD8(+) memory T-cell responses. Any putative limitations of this cross-sectional study will certainly be answered by the ongoing longitudinal population-based investigation. Overall, our data support the current Brazilian national immunization policy guidelines that recommend one booster dose 10 y after primary 17DD-YF vaccination.
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Campi-Azevedo
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Christiane
authors.initials: C
authors.lastname: Costa-Pereira
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Lis R
authors.initials: LR
authors.lastname: Antonelli
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Cristina T
authors.initials: CT
authors.lastname: Fonseca
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Andréa
authors.initials: A
authors.lastname: Teixeira-Carvalho
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Villela-Rezende
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Raiany A
authors.initials: RA
authors.lastname: Santos
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Maurício A
authors.initials: MA
authors.lastname: Batista
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Fernanda M
authors.initials: FM
authors.lastname: Campos
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Luiza
authors.initials: L
authors.lastname: Pacheco-Porto
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Otoni A
authors.initials: OA
authors.lastname: Melo Júnior
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Débora M S H
authors.initials: DM
authors.lastname: Hossell
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Jordana G
authors.initials: JG
authors.lastname: Coelho-dos-Reis
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Peruhype-Magalhães
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Matheus F
authors.initials: MF
authors.lastname: Costa-Silva
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Jaquelline G
authors.initials: JG
authors.lastname: de Oliveira
authors.affiliation: b Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos-FIOCRUZ ; Rio de Janeiro , Brazil.
authors.forename: Roberto H
authors.initials: RH
authors.lastname: Farias
authors.affiliation: b Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos-FIOCRUZ ; Rio de Janeiro , Brazil.
authors.forename: Tatiana G
authors.initials: TG
authors.lastname: Noronha
authors.affiliation: c Secretaria de Estado de Saúde; Governo do Estado de Minas Gerais; Belo Horizonte ; Minas Gerais , Brazil.
authors.forename: Jandira A
authors.initials: JA
authors.lastname: Lemos
authors.affiliation: b Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos-FIOCRUZ ; Rio de Janeiro , Brazil.
authors.forename: Vanessa dos R
authors.initials: Vdos R
authors.lastname: von Doellinger
authors.affiliation: b Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos-FIOCRUZ ; Rio de Janeiro , Brazil.
authors.forename: Marisol
authors.initials: M
authors.lastname: Simões
authors.affiliation: b Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos-FIOCRUZ ; Rio de Janeiro , Brazil.
authors.forename: Mirian M
authors.initials: MM
authors.lastname: de Souza
authors.affiliation: d Universidade Federal de Alfenas ; Alfenas , Minas Gerais , Brazil.
authors.forename: Luiz C
authors.initials: LC
authors.lastname: Malaquias
authors.affiliation: e Instituto de Biologia do Exército ; Rio de Janeiro , Brazil.
authors.forename: Harold R
authors.initials: HR
authors.lastname: Persi
authors.affiliation: e Instituto de Biologia do Exército ; Rio de Janeiro , Brazil.
authors.forename: Jorge M
authors.initials: JM
authors.lastname: Pereira
authors.affiliation: e Instituto de Biologia do Exército ; Rio de Janeiro , Brazil.
authors.forename: José A
authors.initials: JA
authors.lastname: Martins
authors.affiliation: e Instituto de Biologia do Exército ; Rio de Janeiro , Brazil.
authors.forename: Marcos
authors.initials: M
authors.lastname: Dornelas-Ribeiro
authors.affiliation: e Instituto de Biologia do Exército ; Rio de Janeiro , Brazil.
authors.forename: Aline de A
authors.initials: Ade A
authors.lastname: Vinhas
authors.affiliation: e Instituto de Biologia do Exército ; Rio de Janeiro , Brazil.
authors.forename: Tatiane R
authors.initials: TR
authors.lastname: Alves
authors.affiliation: f Assessoria Clínica de Bio-Manguinhos; FIOCRUZ ; Rio de Janeiro , Brazil.
authors.forename: Maria de L
authors.initials: Mde L
authors.lastname: Maia
authors.affiliation: b Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos-FIOCRUZ ; Rio de Janeiro , Brazil.
authors.forename: Marcos da S
authors.initials: Mda S
authors.lastname: Freire
authors.affiliation: b Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos-FIOCRUZ ; Rio de Janeiro , Brazil.
authors.forename: Reinaldo de M
authors.initials: Rde M
authors.lastname: Martins
authors.affiliation: f Assessoria Clínica de Bio-Manguinhos; FIOCRUZ ; Rio de Janeiro , Brazil.
authors.forename: Akira
authors.initials: A
authors.lastname: Homma
authors.affiliation: g Secretaria de Vigilância em Saúde ; Ministério da Saúde , Brazil.
authors.forename: Alessandro P M
authors.initials: AP
authors.lastname: Romano
authors.affiliation: g Secretaria de Vigilância em Saúde ; Ministério da Saúde , Brazil.
authors.forename: Carla M
authors.initials: CM
authors.lastname: Domingues
authors.affiliation: h Universidade de Brasília ; Distrito Federal , Brazil.
authors.forename: Pedro L
authors.initials: PL
authors.lastname: Tauil
authors.affiliation: i Instituto Evandro Chagas-IEC ; Ananindeua , Pará , Brazil.
authors.forename: Pedro F
authors.initials: PF
authors.lastname: Vasconcelos
authors.affiliation: j Center for Biologics Evaluation and Research-CBER; US Food and Drug Administration (FDA) ; Silver Spring , MD , USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Rios
authors.affiliation: k Diretoria Regional de Brasília-Direb; FIOCRUZ ; Brasília , Brazil.
authors.forename: Iramaya R
authors.initials: IR
authors.lastname: Caldas
authors.affiliation: l Escola Nacional de Saúde Pública; FIOCRUZ ; Rio de Janeiro , Brazil.
authors.forename: Luiz A
authors.initials: LA
authors.lastname: Camacho
authors.affiliation: a Centro de Pesquisas René Rachou; FIOCRUZ ; Minas Gerais , Brazil.
authors.forename: Olindo Assis
authors.initials: OA
authors.lastname: Martins-Filho
mesh_terms: D057134:Antibodies, Neutralizing / Q000097:blood* / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001938:Brazil; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014611:Vaccination; D015004:Yellow Fever / Q000517:prevention & control* / Q000821:virology; D022341:Yellow Fever Vaccine / Q000276:immunology*; D015005:Yellow fever virus / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D014409:Tumor Necrosis Factor-alpha; D022341:Yellow Fever Vaccine; D007371:Interferon-gamma
keywords: cytokine; duration of immunity; flow cytometry; memory cells and vaccination; vaccine; yellow fever