title: mTOR signaling controls VGLUT2 expression to maintain pain hypersensitivity after tissue injury.
abstract: Mammalian target of rapamycin (mTOR) is a serine-threonine protein kinase that controls protein synthesis in the nervous system. Here, we characterized the role of protein synthesis regulation due to mTOR signaling in rat dorsal root ganglion (DRG) following plantar incision. The number of phosphorylated mTOR (p-mTOR)-positive neurons was increased 2-4days after the incision. Rapamycin inhibited p-mTOR expression in the DRG and thermal hypersensitivity 3days but not 1day after the incision. Vesicular glutamate transporter 2 (VGLUT2) expression was increased after the plantar incision, which was inhibited by rapamycin. These results demonstrated that tissue injury induces phosphorylation of mTOR and increased protein level of VGLUT2 in the DRG neurons. mTOR phosphorylation involves in maintenance of injury-induced thermal hypersensitivity.
authors.affiliation: Department of Anesthesiology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Izumi
authors.affiliation: Department of Anesthesiology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sasaki
authors.affiliation: Department of Anesthesiology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hashimoto
authors.affiliation: Department of Anesthesiology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sawa
authors.affiliation: Department of Anesthesiology, Kyoto Prefectural University of Medicine, Japan. Electronic address: ama@koto.kpu-m.ac.jp.
authors.forename: F
authors.initials: F
authors.lastname: Amaya
mesh_terms: D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D004195:Disease Models, Animal; D018409:Foot Injuries; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D006614:Hindlimb / Q000293:injuries; D006358:Hot Temperature; D006930:Hyperalgesia / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D010146:Pain / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D017288:Pain Threshold / Q000187:drug effects / Q000502:physiology; D010766:Phosphorylation; D020671:Posterior Horn Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D020123:Sirolimus / Q000494:pharmacology; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D050916:TRPV Cation Channels / Q000378:metabolism; D050599:Vesicular Glutamate Transport Protein 2 / Q000378:metabolism*
chemical_list: D000700:Analgesics; C493475:Slc17a6 protein, rat; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; D050599:Vesicular Glutamate Transport Protein 2; C546845:mTOR protein, rat; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus
keywords: VGLUT2; hyperalgesia; incision; mTOR