title: BALB/c and C57BL/6 Mice Differ in Polyreactive IgA Abundance, which Impacts the Generation of Antigen-Specific IgA and Microbiota Diversity.
abstract: The interrelationship between IgAs and microbiota diversity is still unclear. Here we show that BALB/c mice had higher abundance and diversity of IgAs than C57BL/6 mice and that this correlated with increased microbiota diversity. We show that polyreactive IgAs mediated the entrance of non-invasive bacteria to Peyer's patches, independently of CX3CR1(+) phagocytes. This allowed the induction of bacteria-specific IgA and the establishment of a positive feedback loop of IgA production. Cohousing of mice or fecal transplantation had little or no influence on IgA production and had only partial impact on microbiota composition. Germ-free BALB/c, but not C57BL/6, mice already had polyreactive IgAs that influenced microbiota diversity and selection after colonization. Together, these data suggest that genetic predisposition to produce polyreactive IgAs has a strong impact on the generation of antigen-specific IgAs and the selection and maintenance of microbiota diversity.
authors.affiliation: Department of Experimental Oncology and Immunotherapy Programme, IEO, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Floris
authors.initials: F
authors.lastname: Fransen
authors.affiliation: Department of Experimental Oncology and Immunotherapy Programme, IEO, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Zagato
authors.affiliation: Department of Experimental Oncology and Immunotherapy Programme, IEO, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Mazzini
authors.affiliation: Institute of Biomembranes and Bioenergetics, Consiglio Nazionale delle Ricerche, Via Amendola 165/A, 70126 Bari, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Fosso
authors.affiliation: Institute of Biomembranes and Bioenergetics, Consiglio Nazionale delle Ricerche, Via Amendola 165/A, 70126 Bari, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Manzari
authors.affiliation: Department of Experimental Oncology and Immunotherapy Programme, IEO, Via Adamello 16, 20139 Milan, Italy; Groningen Biomolecular Sciences and Biotechnology, Faculty of Mathematics and Natural Sciences, Nijenborgh 7, 9700 CC Groningen, the Netherlands.
authors.forename: Sahar
authors.initials: S
authors.lastname: El Aidy
authors.affiliation: Department of Experimental Oncology and Immunotherapy Programme, IEO, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Chiavelli
authors.affiliation: Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari, Via Orabona 4, 70125 Bari, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: D'Erchia
authors.affiliation: Institute of Immunology, Hannover Medical School, Carl-Neuberg-Stra. 1, 30625 Hannover, Germany.
authors.forename: Maya K
authors.initials: MK
authors.lastname: Sethi
authors.affiliation: Institute of Molecular Medicine, RWTH Aachen University, Carl-Neuberg-Str. 1, 52074 Aachen, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Pabst
authors.affiliation: Institute of Biomembranes and Bioenergetics, Consiglio Nazionale delle Ricerche, Via Amendola 165/A, 70126 Bari, Italy.
authors.forename: Marinella
authors.initials: M
authors.lastname: Marzano
authors.affiliation: Department of Experimental Medicine, University of Perugia, Polo Unico Sant'Andrea delle Fratte, 06132 Perugia, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Moretti
authors.affiliation: Department of Experimental Medicine, University of Perugia, Polo Unico Sant'Andrea delle Fratte, 06132 Perugia, Italy.
authors.forename: Luigina
authors.initials: L
authors.lastname: Romani
authors.affiliation: Department of Experimental Oncology and Immunotherapy Programme, IEO, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Penna
authors.affiliation: Institute of Biomembranes and Bioenergetics, Consiglio Nazionale delle Ricerche, Via Amendola 165/A, 70126 Bari, Italy; Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari, Via Orabona 4, 70125 Bari, Italy.
authors.forename: Graziano
authors.initials: G
authors.lastname: Pesole
authors.affiliation: Department of Experimental Oncology and Immunotherapy Programme, IEO, Via Adamello 16, 20139 Milan, Italy; Dipartimento di Scienze della salute, Universita' di Milano, via Rudini 8, 20142 Milan, Italy. Electronic address: maria.rescigno@ieo.eu.
authors.forename: Maria
authors.initials: M
authors.lastname: Rescigno
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000276:immunology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005434:Flow Cytometry; D014644:Genetic Variation / Q000276:immunology*; D054884:Host-Pathogen Interactions / Q000276:immunology; D007114:Immunization; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D056186:Metagenomics / Q000379:methods; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D064307:Microbiota / Q000235:genetics / Q000276:immunology*; D010581:Peyer's Patches / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology / Q000502:physiology; D013045:Species Specificity
chemical_list: D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D007070:Immunoglobulin A; D012336:RNA, Ribosomal, 16S