title: PCR-based INDEL markers co-dominant between Oryza sativa, japonica cultivars and closely-related wild Oryza species.
abstract: Wild relatives genetically close to cultivars are precious genetic resources for plant breeding. Oryza rufipogon, O. barthii, O. glumaepatula, O. meridionalis and O. longistaminata are such wild species, and are also categorized as AA genome species based on their structural similarities. Chromosome segment substitution lines (CSSLs) are a powerful resource in breeding and genetics, and numerous rice CSSLs have been produced. This study aimed to develop DNA markers for evaluation of CSSLs directly by PCR and subsequent gel electrophoresis. We confirmed that up to 155 of 188 markers developed for detection of japonica-indica INDELs could also detect INDELs between rice cultivars and wild AA-species accessions. Percentages of applicable markers were higher in O. rufipogon accessions (61.7 to 85.6%), and lower in accessions of other four AA species (39.8 to 51.4%). These markers were distributed throughout the rice chromosomes, and will be useful for genotyping of CSSLs and other genetic resources derived from crosses between rice cultivars and closely related wild species.
authors.affiliation: Plant Genetics Laboratory, National Institute of Genetics , Mishima, Shizuoka 411-8540 , Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Niihama
authors.affiliation: Plant Genetics Laboratory, National Institute of Genetics , Mishima, Shizuoka 411-8540 , Japan.
authors.forename: Misato
authors.initials: M
authors.lastname: Mochizuki
authors.affiliation: Plant Genetics Laboratory, National Institute of Genetics , Mishima, Shizuoka 411-8540 , Japan ; Department of Life Science, SOKENDAI (Graduate University for Advanced Studies) , Mishima, Shizuoka 411-8540 , Japan.
authors.forename: Nori
authors.initials: N
authors.lastname: Kurata
authors.affiliation: Department of Life Science, SOKENDAI (Graduate University for Advanced Studies) , Mishima, Shizuoka 411-8540 , Japan ; Experimental Farm, National Institute of Genetics , Mishima, Shizuoka 411-8540 , Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Nonomura
keywords: AA genome; genetic resource; insertion/deletion marker; rice; wild species