title: Sensing Protein Surfaces with Targeted Fluorescent Receptors.
abstract: A methodology for creating fluorescent molecular sensors that respond to changes that occur on the surfaces of specific proteins is presented. This approach, which relies on binding cooperatively between a specific His-tag binder and a nonspecific protein-surface receptor, enabled the development of a sensor that can track changes on the surface of a His-tag-labeled calmodulin (His-CaM) upon interacting with metal ions, small molecules, and protein binding partners. The way this approach was used to detect dephosphorylation of an unlabeled calmodulin-dependent protein kinase II (CaMKII), and the binding of Bax BH3 to His-tagged B-cell lymphoma 2 (Bcl-2) protein is also presented.
authors.affiliation: Department of Organic Chemistry, Weizmann Institute of Science, 76100, Rehovot (Israel).
authors.forename: Yael
authors.initials: Y
authors.lastname: Nissinkorn
authors.affiliation: Department of Organic Chemistry, Weizmann Institute of Science, 76100, Rehovot (Israel).
authors.forename: Naama
authors.initials: N
authors.lastname: Lahav-Mankovski
authors.affiliation: Department of Biological Services, Weizmann Institute of Science, 76100, Rehovot (Israel).
authors.forename: Aharon
authors.initials: A
authors.lastname: Rabinkov
authors.affiliation: Israel Structural Proteomics Center, Weizmann Institute of Science, 76100, Rehovot (Israel).
authors.forename: Shira
authors.initials: S
authors.lastname: Albeck
authors.affiliation: Department of Organic Chemistry, Weizmann Institute of Science, 76100, Rehovot (Israel).
authors.forename: Leila
authors.initials: L
authors.lastname: Motiei
authors.affiliation: Department of Organic Chemistry, Weizmann Institute of Science, 76100, Rehovot (Israel). david.margulies@weizmann.ac.il.
authors.forename: David
authors.initials: D
authors.lastname: Margulies
mesh_terms: D001665:Binding Sites; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000737:chemistry* / Q000378:metabolism; D002147:Calmodulin / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D011485:Protein Binding; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D002147:Calmodulin; D008565:Membrane Proteins; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D002118:Calcium
keywords: biomolecular interactions; fluorescent sensors; multivalency; protein-surface recognition; synthetic receptors