title: Lawsonia intracellularis infection of intestinal crypt cells is associated with specific depletion of secreted MUC2 in goblet cells.
abstract: The expression patterns of secreted (MUC2 and MUC5AC) and membrane-tethered (MUC1, MUC4, MUC12 and MUC13) mucins were monitored in healthy pigs and pigs challenged orally with Lawsonia intracellularis. These results showed that the regulation of mucin gene expression is distinctive along the GI tract of the healthy pig, and may reflect an association between the function of the mucin subtypes and different physiological demands at various sites. We identified a specific depletion of secreted MUC2 from goblet cells in infected pigs that correlated with the increased level of intracellular bacteria in crypt cells. We concluded that L. intracellularis may influence MUC2 production, thereby altering the mucus barrier and enabling cellular invasion.
authors.affiliation: The Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK; Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK.
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Bengtsson
authors.affiliation: Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK.
authors.forename: Neil
authors.initials: N
authors.lastname: MacIntyre
authors.affiliation: The Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK; Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK.
authors.forename: Jack
authors.initials: J
authors.lastname: Guthrie
authors.affiliation: The Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK; Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK.
authors.forename: Alison D
authors.initials: AD
authors.lastname: Wilson
authors.affiliation: The Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK; Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK.
authors.forename: Heather
authors.initials: H
authors.lastname: Finlayson
authors.affiliation: The Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK; Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK.
authors.forename: Oswald
authors.initials: O
authors.lastname: Matika
authors.affiliation: The Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK; Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Pong-Wong
authors.affiliation: The Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK; Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK.
authors.forename: Sionagh H
authors.initials: SH
authors.lastname: Smith
authors.affiliation: The Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK; Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK.
authors.forename: Alan L
authors.initials: AL
authors.lastname: Archibald
authors.affiliation: The Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK; Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK. Electronic address: tahar.aitali@roslin.ed.ac.uk.
authors.forename: Tahar
authors.initials: T
authors.lastname: Ait-Ali
mesh_terms: D000818:Animals; D058491:Bacterial Load; D045824:Desulfovibrionaceae Infections / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D005786:Gene Expression Regulation; D020397:Goblet Cells / Q000378:metabolism / Q000382:microbiology; D007082:Ileum / Q000378:metabolism / Q000382:microbiology; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology; D020639:Lawsonia Bacteria / Q000472:pathogenicity; D055262:Mucin-2 / Q000235:genetics / Q000378:metabolism*; D009077:Mucins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034421:Sus scrofa; D013552:Swine / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013553:Swine Diseases
chemical_list: D055262:Mucin-2; D009077:Mucins; D012333:RNA, Messenger
keywords: Goblet cells; Lawsonia intracellularis; Mucin; Pig