title: Measuring Meiotic Crossovers via Multi-Locus Genotyping of Single Pollen Grains in Barley.
abstract: The detection of meiotic crossovers in crop plants currently relies on scoring DNA markers in a segregating population or cytological visualization. We investigated the feasibility of using flow-sorted haploid nuclei, Phi29 DNA polymerase-based whole-genome-amplification (WGA) and multi-locus KASP-genotyping to measure meiotic crossovers in individual barley pollen grains. To demonstrate the proof of concept, we used 24 gene-based physically mapped single nucleotide polymorphisms to genotype the WGA products of 50 single pollen nuclei. The number of crossovers per chromosome, recombination frequencies along chromosome 3H and segregation distortion were analysed and compared to a doubled haploid (DH) population of the same genotype. The number of crossovers and chromosome wide recombination frequencies show that this approach is able to produce results that resemble those obtained from other methods in a biologically meaningful way. Only the segregation distortion was found to be lower in the pollen population than in DH plants.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstrasse 3, 06466 Stadt Seeland, Germany.
authors.forename: Steven
authors.initials: S
authors.lastname: Dreissig
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstrasse 3, 06466 Stadt Seeland, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Fuchs
authors.affiliation: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, Olomouc CZ-78371, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Cápal
authors.affiliation: KWS-UK Ltd, 56 Church Street, Thriplow, Hertfordshire, SG8 7RE, United Kingdom.
authors.forename: Nicola
authors.initials: N
authors.lastname: Kettles
authors.affiliation: KWS-UK Ltd, 56 Church Street, Thriplow, Hertfordshire, SG8 7RE, United Kingdom.
authors.forename: Ed
authors.initials: E
authors.lastname: Byrne
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstrasse 3, 06466 Stadt Seeland, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Houben
mesh_terms: D032461:Chromosomes, Plant; D003434:Crossing Over, Genetic; D018744:DNA, Plant; D018745:Genome, Plant; D006238:Haploidy; D001467:Hordeum / Q000235:genetics*; D008540:Meiosis / Q000235:genetics*; D058885:Multilocus Sequence Typing; D011058:Pollen / Q000235:genetics*; D040641:Quantitative Trait Loci; D011995:Recombination, Genetic
chemical_list: D018744:DNA, Plant