title: Novel bocaparvoviruses in rabbits.
abstract: Bocaparvovirus is a newly established genus within the family Parvoviridae and has been identified as a possible cause of enteric, respiratory, reproductive/neonatal and neurological disease in humans and several animal species. In this study, metagenomic analysis was used to identify and characterise a novel bocaparvovirus in the faeces of rabbits with enteric disease. To assess the prevalence of the novel virus, rectal swabs and faecal samples obtained from rabbits with and without diarrhoea were screened with a specific PCR assay. The complete genome sequence of the novel parvovirus was reconstructed. The virus was distantly related to other bocaparvoviruses; the three ORFs shared 53%, 53% and 50% nucleotide identity, respectively, to homologous genes of porcine bocaparvoviruses. The virus was detected in 8/29 (28%) and 16/95 (17%) samples of rabbits with and without diarrhoea, respectively. Sequencing of the capsid protein fragment targeted by the diagnostic PCR identified two distinct bocaparvovirus populations/sub-types, with 91.7-94.5% nucleotide identity to each other. Including these novel parvoviruses in diagnostic algorithms of rabbit diseases might help inform their potential pathogenic role and impact on rabbit production and the virological profiles of laboratory rabbits.
authors.affiliation: Department of Veterinary Medicine, University Aldo Moro of Bari, Km 3 St. for Casamassima, Valenzano 70010, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Lanave
authors.affiliation: Department of Veterinary Medicine, University Aldo Moro of Bari, Km 3 St. for Casamassima, Valenzano 70010, Italy. Electronic address: vito.martella@uniba.it.
authors.forename: V
authors.initials: V
authors.lastname: Martella
authors.affiliation: Institute for Veterinary Medical Research, Hungarian Academy of Sciences, 21 Hungaria St., Budapest H-1143, Hungary.
authors.forename: S L
authors.initials: SL
authors.lastname: Farkas
authors.affiliation: Institute for Veterinary Medical Research, Hungarian Academy of Sciences, 21 Hungaria St., Budapest H-1143, Hungary.
authors.forename: S
authors.initials: S
authors.lastname: Marton
authors.affiliation: Institute for Veterinary Medical Research, Hungarian Academy of Sciences, 21 Hungaria St., Budapest H-1143, Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Fehér
authors.affiliation: Department of Veterinary Medicine, University Aldo Moro of Bari, Km 3 St. for Casamassima, Valenzano 70010, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Bodnar
authors.affiliation: Experimental Zooprophylactic Institute (IZS) of Lombardia and Emilia Romagna, 7/9 Bianchi St., Brescia 25124, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Lavazza
authors.affiliation: Department of Veterinary Medicine, University Aldo Moro of Bari, Km 3 St. for Casamassima, Valenzano 70010, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Decaro
authors.affiliation: Department of Veterinary Medicine, University Aldo Moro of Bari, Km 3 St. for Casamassima, Valenzano 70010, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Buonavoglia
authors.affiliation: Institute for Veterinary Medical Research, Hungarian Academy of Sciences, 21 Hungaria St., Budapest H-1143, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Bányai
mesh_terms: D000818:Animals; D016679:Genome, Viral; D010321:Parvoviridae / Q000235:genetics; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D011817:Rabbits; D014776:Virus Cultivation
keywords: Bocaparvovirus; Diarrhoea; Parvoviridae; Rabbit