title: [IL-18 and TNF-α promote the expression of IL-18Rβ in 3T3-L1 adipocytes and RAW264.7 macrophages].
abstract: OBJECTIVE
  To explore the effects of interleukin 18 (IL-18) and tumor necrosis factor alpha (TNF-α) on the expression of IL-18 receptor beta (IL-18Rβ) in 3T3-L1 adipocytes and RAW264.7 macrophages.
  
  
  METHODS
  The level of IL-18Rβ was determined by reverse transcription PCR and Western blotting in both 3T3-L1 adipocytes and RAW264.7 macrophages after processed by different concentrations of IL-18 and TNF-α.
  
  
  RESULTS
  The level of IL-18Rβ mRNA and protein significantly increased in 3T3-L1 adipocytes after treated with 10 ng/mL, 100 ng/mL TNF-α. The expression of IL-18Rβ was also raised in RAW264.7 macrophages after treated with 10 ng/mL, 100 ng/mL IL-18.
  
  
  CONCLUSIONS
  IL-18Rβ could be up-regulated by IL-18 or TNF-α in 3T3-L1 adipocytes and RAW264.7 macrophages. IL-18 may be an important factor involved in the inflammation of adipose tissues in diabetes and obesity.
authors.affiliation: Department of Endocrinology, First Affiliated Hospital, Bengbu Medical College, Bengbu 233000, China.
authors.forename: Guoxi
authors.initials: G
authors.lastname: Jin
authors.affiliation: Department of Endocrinology, First Affiliated Hospital, Bengbu Medical College, Bengbu 233000, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, First Affiliated Hospital, Bengbu Medical College, Bengbu 233000, China.
authors.forename: Yaxin
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Department of Endocrinology, First Affiliated Hospital, Bengbu Medical College, Bengbu 233000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000276:immunology; D000818:Animals; D020382:Interleukin-18 / Q000494:pharmacology*; D053728:Interleukin-18 Receptor beta Subunit / Q000032:analysis / Q000235:genetics*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: C501223:IL18rap protein, mouse; D020382:Interleukin-18; D053728:Interleukin-18 Receptor beta Subunit; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha