title: High-Throughput Tag-Sequencing Analysis of Early Events Induced by Ochratoxin A in HepG-2 Cells.
abstract: Ochratoxin A (OTA) is produced by fungi of the species Aspergillus and Penicillium. OTA has displayed hepatotoxicity in mammals. Although recent studies have indicated that OTA influences liver function, little is known regarding its impact on differential early liver toxicity. In this study, we report high-throughput tag-sequencing (Tag-seq) analysis of the transcriptome using Solexa Analyzer platform after 4 h of OTA treatment on HepG-2 cells. The analyses of differentially expressed genes revealed the substantial changes. A total of 21,449 genes were identified and quantified, with 2726 displaying significantly altered expression levels. Expression level data were then integrated with a network of gene-gene interactions, and biological pathways to obtain a systems-level view of changes in the transcriptome that occur with OTA resistance. Our data suggest that OTA exposure leads to an imbalance in zinc finger expression and shed light on splicing factor and mitochondrial-based mechanisms.
authors.affiliation: Laboratory of Food Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People's Republic of China. xuwentao@cau.edu.cn.|Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University (BTBU), Beijing, 100048, People's Republic of China. xuwentao@cau.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Food Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People's Republic of China.
authors.forename: Xiaozhe
authors.initials: X
authors.lastname: Qi
authors.affiliation: Laboratory of Food Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People's Republic of China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Laboratory of Food Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People's Republic of China.|The Supervision, Inspection & Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Beijing, 100083, People's Republic of China.
authors.forename: YunBo
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Laboratory of Food Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People's Republic of China.|The Supervision, Inspection & Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Beijing, 100083, People's Republic of China.
authors.forename: Kunlun
authors.initials: K
authors.lastname: Huang
authors.affiliation: Laboratory of Food Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People's Republic of China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Xu
mesh_terms: D056945:Hep G2 Cells; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects; D009793:Ochratoxins / Q000494:pharmacology*; D012326:RNA Splicing / Q000187:drug effects; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A
keywords: Cytotoxicity; High-throughput tag-sequencing; Ochratoxin A