title: [Effects of LPS-stimulated monocyte culture supernatant on the OPG/RANKL of osteoblastic cells].
abstract: OBJECTIVE
  To investigate the influence of Pg-LPS stimulated monocyte (RAW264.7) culture supernatant on the OPG/RANKL expression of osteoblastic cells (MC3T3-E1).
  
  
  METHODS
  The culture supernatant of monocytes stimulated with Pg-LPS was applied to osteoblasts MC3T3-E1 with different diluted concentrations (10%, 20%, 30%, 40% and 50%) simultaneously for 24h, then RT-PCR was used to detect the expression changes of OPG/RANKL mRNA. Western blot was used to detect the expression changes of OPG/RANKL protein. The data was analyzed by ANOVA using SPSS 17.0 software package.
  
  
  RESULTS
  After stimulation of different concentrations of inflammatory supernatant, the expressions of OPGmRNA and protein significantly decreased (P<0.05), whereas the expressions of RANKLmRNA and protein significantly increased(P<0.05), both of them were in a concentration-dependent manner.
  
  
  CONCLUSIONS
  These results indicate that Pg-LPS stimulated RAW264.7 culture supernatant can inhibit the osteogenesis and differentiation of the osteoblasts through inhibiting the expression of OPGmRNA and protein of osteoblastic cells, while increasing the expression of RANKLmRNA and protein in a concentration-dependent manner.
authors.affiliation: Department of Orthodontics, School and Hospital of Stomatology, Wenzhou Medical University. Wenzhou 325027, Zhejiang Province, China.E-mail:179402394@qq.com.
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Hua-xiang
authors.initials: HX
authors.lastname: Qin
authors.forename: Hui
authors.initials: H
authors.lastname: Deng
authors.forename: Chao-fan
authors.initials: CF
authors.lastname: Sun
authors.forename: Rong-dang
authors.initials: RD
authors.lastname: Hu
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D006801:Humans; D008070:Lipopolysaccharides; D009000:Monocytes; D010006:Osteoblasts / Q000502:physiology*; D010012:Osteogenesis; D053244:Osteoprotegerin; D053245:RANK Ligand / Q000378:metabolism*; D012333:RNA, Messenger
chemical_list: D008070:Lipopolysaccharides; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; C505688:TNFSF11 protein, human