title: Anti-irritant and anti-inflammatory effects of glycerol and xylitol in sodium lauryl sulphate-induced acute irritation.
abstract: BACKGROUND
  Glycerol is known to possess anti-irritant and hydrating properties and previous studies suggested that xylitol may also have similar effects.
  
  
  OBJECTIVE
  Our aim was to study whether different concentrations of these polyols restore skin barrier function and soothe inflammation in sodium lauryl sulphate (SLS)-induced acute irritation.
  
  
  METHODS
  The experiments were performed on male SKH-1 hairless mice. The skin of the dorsal region was exposed to SLS (5%) for 3 h alone or together with 5% or 10% of glycerol respectively. Further two groups received xylitol solutions (8.26% and 16.52% respectively) using the same osmolarities, which were equivalent to those of the glycerol treatments. The control group was treated with purified water. Transepidermal water loss (TEWL) and skin hydration were determined. Microcirculatory parameters of inflammation were observed by means of intravital videomicroscopy (IVM). Furthermore, accumulation of neutrophil granulocytes and lymphocytes, the expression of inflammatory cytokines and SLS penetration were assessed, as well.
  
  
  RESULTS
  Treatment with the 10% of glycerol and both concentrations of xylitol inhibited the SLS-induced elevation of TEWL and moderated the irritant-induced increase in dermal blood flow and in the number of leucocyte-endothelial interactions. All concentrations of the applied polyols improved hydration and prevented the accumulation of lymphocytes near the treatment site. At the mRNA level, neither glycerol nor xylitol influenced the expression of interleukin-1 alpha. However, expression of interleukin-1 beta was significantly decreased by the 10% glycerol treatment, while expression of tumour necrosis factor-alpha decreased upon the same treatment, as well as in response to xylitol. Higher polyol treatments decreased the SLS penetration to the deeper layers of the stratum corneum.
  
  
  CONCLUSIONS
  Both of the analysed polyols exert considerable anti-irritant and anti-inflammatory properties, but the effective concentration of xylitol is lower than that of glycerol.
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Szél
authors.affiliation: Dermatological Research Group of the Hungarian Academy of Sciences, University of Szeged, Szeged, Hungary.
authors.forename: H
authors.initials: H
authors.lastname: Polyánka
authors.affiliation: Dermatological Research Group of the Hungarian Academy of Sciences, University of Szeged, Szeged, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Szabó
authors.affiliation: Institute of Surgical Research, University of Szeged, Szeged, Hungary.
authors.forename: P
authors.initials: P
authors.lastname: Hartmann
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary.
authors.forename: D
authors.initials: D
authors.lastname: Degovics
authors.affiliation: Department of Pharmaceutical Technology, University of Szeged, Szeged, Hungary.
authors.forename: B
authors.initials: B
authors.lastname: Balázs
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary.
authors.forename: I B
authors.initials: IB
authors.lastname: Németh
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary.
authors.forename: C
authors.initials: C
authors.lastname: Korponyai
authors.affiliation: Department of Pharmaceutical Technology, University of Szeged, Szeged, Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Csányi
authors.affiliation: Institute of Surgical Research, University of Szeged, Szeged, Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Kaszaki
authors.affiliation: Unit of Cell Pharmacology, Hebrew University, Jerusalem, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Dikstein
authors.affiliation: Department of Oral Surgery, University of Szeged, Szeged, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Nagy
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary.|Dermatological Research Group of the Hungarian Academy of Sciences, University of Szeged, Szeged, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: Kemény
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary.|Department of Oral Biology and Experimental Dental Research, University of Szeged, Szeged, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Erős
mesh_terms: D000818:Animals; D017453:Dermatitis, Irritant / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D004643:Emollients / Q000494:pharmacology / Q000627:therapeutic use*; D015870:Gene Expression / Q000187:drug effects; D005990:Glycerol / Q000494:pharmacology / Q000627:therapeutic use*; D053582:Interleukin-1alpha / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D000069416:Intravital Microscopy; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D010539:Permeability / Q000187:drug effects; D012039:Regional Blood Flow / Q000187:drug effects; D012867:Skin / Q000098:blood supply / Q000737:chemistry / Q000378:metabolism*; D012879:Skin Physiological Phenomena / Q000187:drug effects*; D012967:Sodium Dodecyl Sulfate / Q000493:pharmacokinetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D014867:Water / Q000032:analysis; D014870:Water Loss, Insensible / Q000187:drug effects; D014993:Xylitol / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D004643:Emollients; D053582:Interleukin-1alpha; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D014867:Water; D012967:Sodium Dodecyl Sulfate; D005990:Glycerol; D014993:Xylitol