title: Identification of key residues in Debaryomyces hansenii carbonyl reductase for highly productive preparation of (S)-aryl halohydrins.
abstract: Key residues of Debaryomyces hansenii carbonyl reductase in the determination of the reducing activity towards aryl haloketones were identified through combinatorial mutation of conserved residues. This study provides a green and efficient biocatalyst for the synthesis of (S)-aryl halohydrins.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, and Shanghai Collaborative Innovation Center, East China University of Science and Technology, for Biomanufacturing, Shanghai 200237, People's Republic of China. huileiyu@ecust.edu.cn jianhexu@ecust.edu.cn.
authors.forename: Guo-Chao
authors.initials: GC
authors.lastname: Xu
authors.forename: Yue-Peng
authors.initials: YP
authors.lastname: Shang
authors.forename: Hui-Lei
authors.initials: HL
authors.lastname: Yu
authors.forename: Jian-He
authors.initials: JH
authors.lastname: Xu
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000235:genetics; D002384:Catalysis; D002728:Chlorohydrins / Q000138:chemical synthesis*; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D009154:Mutation; D004718:Saccharomycetales / Q000201:enzymology* / Q000235:genetics
chemical_list: D002728:Chlorohydrins; D005656:Fungal Proteins; D000429:Alcohol Oxidoreductases