title: A reference gene set for chemosensory receptor genes of Manduca sexta.
abstract: The order of Lepidoptera has historically been crucial for chemosensory research, with many important advances coming from the analysis of species like Bombyx mori or the tobacco hornworm, Manduca sexta. Specifically M. sexta has long been a major model species in the field, especially regarding the importance of olfaction in an ecological context, mainly the interaction with its host plants. In recent years transcriptomic data has led to the discovery of members of all major chemosensory receptor families in the species, but the data was fragmentary and incomplete. Here we present the analysis of the newly available high-quality genome data for the species, supplemented by additional transcriptome data to generate a high quality reference gene set for the three major chemosensory receptor gene families, the gustatory (GR), olfactory (OR) and antennal ionotropic receptors (IR). Coupled with gene expression analysis our approach allows association of specific receptor types and behaviors, like pheromone and host detection. The dataset will provide valuable support for future analysis of these essential chemosensory modalities in this species and in Lepidoptera in general.
authors.affiliation: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745 Jena, Germany. Electronic address: ckoenig@ice.mpg.de.
authors.forename: Christopher
authors.initials: C
authors.lastname: Koenig
authors.affiliation: Department of Microbiology, Icahn School of Medicine at Mount Sinai, 1468 Madison Avenue, New York, NY 10029, USA. Electronic address: ariana.hirsh@mssm.edu.
authors.forename: Ariana
authors.initials: A
authors.lastname: Hirsh
authors.affiliation: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745 Jena, Germany. Electronic address: sbucks@ice.mpg.de.
authors.forename: Sascha
authors.initials: S
authors.lastname: Bucks
authors.affiliation: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745 Jena, Germany. Electronic address: cklinner@ice.mpg.de.
authors.forename: Christian
authors.initials: C
authors.lastname: Klinner
authors.affiliation: Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745 Jena, Germany. Electronic address: hvogel@ice.mpg.de.
authors.forename: Heiko
authors.initials: H
authors.lastname: Vogel
authors.affiliation: Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur Boston, MA 02115, USA. Electronic address: ashukla@fas.harvard.edu.
authors.forename: Aditi
authors.initials: A
authors.lastname: Shukla
authors.affiliation: Department of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY 10027, USA. Electronic address: jmansfie@barnard.edu.
authors.forename: Jennifer H
authors.initials: JH
authors.lastname: Mansfield
authors.affiliation: Department of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY 10027, USA. Electronic address: bmorton@barnard.edu.
authors.forename: Brian
authors.initials: B
authors.lastname: Morton
authors.affiliation: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745 Jena, Germany. Electronic address: hansson@ice.mpg.de.
authors.forename: Bill S
authors.initials: BS
authors.lastname: Hansson
authors.affiliation: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745 Jena, Germany. Electronic address: grosse-wilde@ice.mpg.de.
authors.forename: Ewald
authors.initials: E
authors.lastname: Grosse-Wilde
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D049750:Genome, Insect; D008297:Male; D018402:Manduca / Q000235:genetics* / Q000378:metabolism; D005810:Multigene Family; D010802:Phylogeny; D058468:Receptors, Ionotropic Glutamate / Q000235:genetics / Q000378:metabolism; D018035:Receptors, Odorant / Q000235:genetics* / Q000378:metabolism; D043563:Receptors, Pheromone / Q000235:genetics / Q000378:metabolism; D012903:Smell / Q000235:genetics; D059467:Transcriptome / Q000235:genetics
chemical_list: D058468:Receptors, Ionotropic Glutamate; D018035:Receptors, Odorant; D043563:Receptors, Pheromone
keywords: Gustatory receptor; Ionotropic receptor; Lepidoptera; Olfaction; Pheromone receptor; Taste