title: [Preparation and application of polyclonal antibody against amelotin polypeptide].
abstract: OBJECTIVE
  To prepare and identify the rabbit anti-mouse polyclonal antibody against amelotin polypeptide.
  
  
  METHODS
  The polypeptide was synthesized based on the bioinformatics analysis of amelotin, and coupled with keyhole limpet hemocyanin (KLH) for immunization. The amelotin polypeptide-KLH was injected into New Zealand rabbits to prepare the polyclonal antibody. ELISA technology was used to detect the titer of the antibody. The specificity of the polyclonal antibody was identified by Western blotting. The expression of amelotin in the submandibular tissue of mice was observed by immunohistochemistry.
  
  
  RESULTS
  ELISA showed that the titer of amelotin antibody was 1:1,000,000. Western blotting verified that the antibody had a high specificity. Immunohistochemistry indicated that amelotin was highly expressed in odontoprisis full-thick enamel of 3-day-old and 7-day-old mice, and also expressed in duct epithelial cytoplasm of submandibular glands of 7-day-old mice.
  
  
  CONCLUSIONS
  The polyclonal antibody against amelotin polypeptide has been prepared successfully with high titer and high specificity.
authors.affiliation: Institute of Stomatology, School of Stomotalogy, Weifang Medical University, Weifang 261053, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Stomatology, School of Stomotalogy, Weifang Medical University, Weifang 261053, China.
authors.forename: Honghai
authors.initials: H
authors.lastname: Ji
authors.affiliation: Institute of Stomatology, School of Stomotalogy, Weifang Medical University, Weifang 261053, China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Stomatology, School of Stomotalogy, Weifang Medical University, Weifang 261053, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D003746:Dental Enamel Proteins / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice; D011817:Rabbits
chemical_list: C507067:AMTN protein, human; D000906:Antibodies; D003746:Dental Enamel Proteins