title: GLUT4 translocation is not impaired after acute exercise in skeletal muscle of women with obesity and polycystic ovary syndrome.
abstract: OBJECTIVE
  The aim of this study was to examine the effects of acute exercise on insulin signaling in skeletal muscle of women with polycystic ovary syndrome (PCOS) and controls (CTRL).
  
  
  METHODS
  Fifteen women with obesity and PCOS and 12 body mass index-matched CTRL participated in this study. Subjects performed a 40-min single bout of exercise. Muscle biopsies were performed before and 60 min after exercise. Selected proteins were assessed by Western blotting.
  
  
  RESULTS
  CTRL, but not PCOS, showed a significant increase in PI3-k p85 and AS160 Thr 642 after a single bout of exercise (P = 0.018 and P = 0.018, respectively). Only PCOS showed an increase in Akt Thr 308 and AMPK phosphorylation after exercise (P = 0.018 and P = 0.018, respectively). Total GLUT4 expression was comparable between groups (P > 0.05). GLUT4 translocation tended to be significantly higher in both groups after exercise (PCOS: P = 0.093; CTRL: P = 0.091), with no significant difference between them (P > 0.05).
  
  
  CONCLUSIONS
  A single bout of exercise elicited similar GLUT4 translocation in skeletal muscle of PCOS and CTRL, despite a slightly differential pattern of protein phosphorylation. The absence of impairment in GLUT4 translocation suggests that PCOS patients with obesity and insulin resistance may benefit from exercise training.
authors.affiliation: School of Physical Education and Sport, Department of Biodynamic of Human Movement, University of Sao Paulo, Brazil.
authors.forename: Wagner Silva
authors.initials: WS
authors.lastname: Dantas
authors.affiliation: Endocrinology Division, School of Medicine, University of Sao Paulo, Brazil.
authors.forename: José Antonio Miguel
authors.initials: JA
authors.lastname: Marcondes
authors.affiliation: Rheumatology Division, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Samuel Katsuyuki
authors.initials: SK
authors.lastname: Shinjo
authors.affiliation: Rheumatology Division, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Luiz Augusto
authors.initials: LA
authors.lastname: Perandini
authors.affiliation: Laboratory of Pain and Signaling, Butantan Institute, São Paulo, Brazil.
authors.forename: Vanessa Olzon
authors.initials: VO
authors.lastname: Zambelli
authors.affiliation: School of Physical Education and Sport, Department of Biodynamic of Human Movement, University of Sao Paulo, Brazil.
authors.forename: Willian Das
authors.initials: WD
authors.lastname: Neves
authors.affiliation: Endocrinology Division, School of Medicine, University of Sao Paulo, Brazil.
authors.forename: Cristiano Roberto Grimaldi
authors.initials: CR
authors.lastname: Barcellos
authors.affiliation: Endocrinology Division, School of Medicine, University of Sao Paulo, Brazil.
authors.forename: Michele Patrocínio
authors.initials: MP
authors.lastname: Rocha
authors.affiliation: Endocrinology Division, School of Medicine, University of Sao Paulo, Brazil.
authors.forename: Viviane Dos Reis Vieira
authors.initials: Vdos R
authors.lastname: Yance
authors.affiliation: School of Physical Education and Sport, Department of Biodynamic of Human Movement, University of Sao Paulo, Brazil.
authors.forename: Renato Tavares Dos Santos
authors.initials: RT
authors.lastname: Pereira
authors.affiliation: School of Physical Education and Sport, Department of Biodynamic of Human Movement, University of Sao Paulo, Brazil.
authors.forename: Igor Hisashi
authors.initials: IH
authors.lastname: Murai
authors.affiliation: Rheumatology Division, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Ana Lucia De Sá
authors.initials: AL
authors.lastname: Pinto
authors.affiliation: School of Physical Education and Sport, Department of Biodynamic of Human Movement, University of Sao Paulo, Brazil.|Rheumatology Division, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Hamilton
authors.initials: H
authors.lastname: Roschel
authors.affiliation: School of Physical Education and Sport, Department of Biodynamic of Human Movement, University of Sao Paulo, Brazil.|Rheumatology Division, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Gualano
mesh_terms: D000328:Adult; D015992:Body Mass Index; D015444:Exercise / Q000502:physiology*; D005260:Female; D051275:Glucose Transporter Type 4 / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance; D018482:Muscle, Skeletal / Q000378:metabolism*; D009765:Obesity / Q000150:complications / Q000378:metabolism*; D010766:Phosphorylation; D011085:Polycystic Ovary Syndrome / Q000150:complications / Q000378:metabolism* / Q000628:therapy*; D021381:Protein Transport; D015398:Signal Transduction / Q000187:drug effects; D055815:Young Adult
chemical_list: D051275:Glucose Transporter Type 4; D007328:Insulin; C495692:SLC2A4 protein, human