title: The Q-soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptor (Q-SNARE) SNAP-47 Regulates Trafficking of Selected Vesicle-associated Membrane Proteins (VAMPs).
abstract: SNAREs constitute the core machinery of intracellular membrane fusion, but vesicular SNAREs localize to specific compartments via largely unknown mechanisms. Here, we identified an interaction between VAMP7 and SNAP-47 using a proteomics approach. We found that SNAP-47 mainly localized to cytoplasm, the endoplasmic reticulum (ER), and ERGIC and could also shuttle between the cytoplasm and the nucleus. SNAP-47 preferentially interacted with the trans-Golgi network VAMP4 and post-Golgi VAMP7 and -8. SNAP-47 also interacted with ER and Golgi syntaxin 5 and with syntaxin 1 in the absence of Munc18a, when syntaxin 1 is retained in the ER. A C-terminally truncated SNAP-47 was impaired in interaction with VAMPs and affected their subcellular distribution. SNAP-47 silencing further shifted the subcellular localization of VAMP4 from the Golgi apparatus to the ER. WT and mutant SNAP-47 overexpression impaired VAMP7 exocytic activity. We conclude that SNAP-47 plays a role in the proper localization and function of a subset of VAMPs likely via regulation of their transport through the early secretory pathway.
authors.affiliation: Membrane Traffic in Health and Disease, INSERM U950, CNRS, UMR 7592, Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris.
authors.forename: Aurelia
authors.initials: A
authors.lastname: Kuster
authors.affiliation: Membrane Traffic in Health and Disease, INSERM U950, CNRS, UMR 7592, Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris.
authors.forename: Sebastien
authors.initials: S
authors.lastname: Nola
authors.affiliation: Protein Mass Spectrometry Laboratory, Institut Curie, 75005 Paris.
authors.forename: Florent
authors.initials: F
authors.lastname: Dingli
authors.affiliation: Membrane Traffic in Health and Disease, INSERM U950, CNRS, UMR 7592, Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris.
authors.forename: Barbara
authors.initials: B
authors.lastname: Vacca
authors.affiliation: Membrane Traffic in Health and Disease, INSERM U950, CNRS, UMR 7592, Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris.
authors.forename: Christian
authors.initials: C
authors.lastname: Gauchy
authors.affiliation: Membrane Traffic in Health and Disease, INSERM U950, CNRS, UMR 7592, Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Beaujouan
authors.affiliation: Hybrigenics, 3-5 Impasse Reille, 75014 Paris, France.
authors.forename: Marcela
authors.initials: M
authors.lastname: Nunez
authors.affiliation: Hybrigenics, 3-5 Impasse Reille, 75014 Paris, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Moncion
authors.affiliation: Protein Mass Spectrometry Laboratory, Institut Curie, 75005 Paris.
authors.forename: Damarys
authors.initials: D
authors.lastname: Loew
authors.affiliation: Hybrigenics, 3-5 Impasse Reille, 75014 Paris, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Formstecher
authors.affiliation: Membrane Traffic in Health and Disease, INSERM U950, CNRS, UMR 7592, Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris. Electronic address: thierry.galli@inserm.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Galli
authors.affiliation: Membrane Traffic in Health and Disease, INSERM U950, CNRS, UMR 7592, Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris.
authors.forename: Veronique
authors.initials: V
authors.lastname: Proux-Gillardeaux
mesh_terms: D000818:Animals; D004285:Dogs; D061985:Madin Darby Canine Kidney Cells; D021381:Protein Transport; D050682:Q-SNARE Proteins / Q000502:physiology*; D050683:R-SNARE Proteins / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D050682:Q-SNARE Proteins; D050683:R-SNARE Proteins
keywords: ERGIC; SNAP-47; SNARE proteins; VAMP; membrane fusion; membrane trafficking; nuclear translocation; protein secretion; vesicles