title: Neutrophil Responses to Sterile Implant Materials.
abstract: In vivo implantation of sterile materials and devices results in a foreign body immune response leading to fibrosis of implanted material. Neutrophils, one of the first immune cells to be recruited to implantation sites, have been suggested to contribute to the establishment of the inflammatory microenvironment that initiates the fibrotic response. However, the precise numbers and roles of neutrophils in response to implanted devices remains unclear. Using a mouse model of peritoneal microcapsule implantation, we show 30-500 fold increased neutrophil presence in the peritoneal exudates in response to implants. We demonstrate that these neutrophils secrete increased amounts of a variety of inflammatory cytokines and chemokines. Further, we observe that they participate in the foreign body response through the formation of neutrophil extracellular traps (NETs) on implant surfaces. Our results provide new insight into neutrophil function during a foreign body response to peritoneal implants which has implications for the development of biologically compatible medical devices.
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Siddharth
authors.initials: S
authors.lastname: Jhunjhunwala
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Aresta-DaSilva
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Katherine
authors.initials: K
authors.lastname: Tang
authors.affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, 02115, United States of America.
authors.forename: David
authors.initials: D
authors.lastname: Alvarez
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Webber
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Benjamin C
authors.initials: BC
authors.lastname: Tang
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Danya M
authors.initials: DM
authors.lastname: Lavin
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Omid
authors.initials: O
authors.lastname: Veiseh
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Joshua C
authors.initials: JC
authors.lastname: Doloff
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Suman
authors.initials: S
authors.lastname: Bose
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Arturo
authors.initials: A
authors.lastname: Vegas
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Minglin
authors.initials: M
authors.lastname: Ma
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Sahay
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Alan
authors.initials: A
authors.lastname: Chiu
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Andrew
authors.initials: A
authors.lastname: Bader
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Erin
authors.initials: E
authors.lastname: Langan
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Sean
authors.initials: S
authors.lastname: Siebert
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Molecular Medicine, Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, Massachusetts, 01605, United States of America.
authors.forename: Dale L
authors.initials: DL
authors.lastname: Greiner
authors.affiliation: Department of Pediatrics, University of Massachusetts Medical School, Worcester, Massachusetts, 01605, United States of America; Department of Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, 01605, United States of America.
authors.forename: Peter E
authors.initials: PE
authors.lastname: Newburger
authors.affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, 02115, United States of America; The Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, 02139, United States of America.
authors.forename: Ulrich H
authors.initials: UH
authors.lastname: von Andrian
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Harvard-MIT Division of Health Science and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Harvard-MIT Division of Health Science and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States of America; Department of Anesthesiology, Boston Children's Hospital, Boston, Massachusetts, 02115, United States of America.
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Anderson
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D065206:Extracellular Traps / Q000276:immunology / Q000378:metabolism; D005355:Fibrosis; D018836:Inflammation Mediators / Q000378:metabolism; D007958:Leukocyte Count; D051379:Mice; D023421:Models, Animal; D020556:Neutrophil Infiltration / Q000276:immunology; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism*; D010587:Phagocytosis / Q000276:immunology; D019736:Prostheses and Implants / Q000009:adverse effects*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators