title: Increased CD38 expression on T lymphocytes as a marker of HIV dissemination into the central nervous system.
abstract: Cross-sectional analysis on 20 HIV-1 patients with neurological symptoms admitted to two infectious disease units. Cut-off of HIV-RNA (VL) was 20 copies/ml for plasma and cerebral spinal fluid (CSF). Flow cytometry was used to analyze the phenotype of circulating and CSF T lymphocytes. CD38 mean fluorescence intensity (MFI) was higher on circulating CD4+T lymphocytes from patients with VL>20 copies/ml in plasma (P=0.001) or CSF (P=0.001). The frequency of circulating CD8+CD38+T cells and CD38 MFI on these cells were higher in patients with VL>20 copies/ml than in those with undetectable plasma VL (P=0.030 and P=0.023). The frequency of CSF CD4+CD38+T, as well as their CD38 and CD95 MFI, were increased in patients with detectable than non-detectable plasma VL (P=0.01, P=0.03, and P=0.05). The % CD38+CD8+T in CSF correlated with time of virological suppression (ρ=-0.462, P=0.040) and the CNS penetration-effectiveness (CPE) score (ρ=-0.467, P=0.038). In conclusion, (a) the expression of CD38+ on both CD4+, CD8+T lymphocytes from peripheral blood and CSF discriminated between viremic and non-viremic patients and (b) T cell activation/apoptosis markers inversely correlated with CPE to remark the importance for therapy to restore immunological functions.
authors.forename: Chiara
authors.initials: C
authors.lastname: Dentone
authors.forename: Daniela
authors.initials: D
authors.lastname: Fenoglio
authors.forename: Eva
authors.initials: E
authors.lastname: Schenone
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cenderello
authors.forename: Roberta
authors.initials: R
authors.lastname: Prinapori
authors.forename: Alessio
authors.initials: A
authors.lastname: Signori
authors.forename: Alessia
authors.initials: A
authors.lastname: Parodi
authors.forename: Francesca
authors.initials: F
authors.lastname: Kalli
authors.forename: Florinda
authors.initials: F
authors.lastname: Battaglia
authors.forename: Marcello
authors.initials: M
authors.lastname: Feasi
authors.forename: Bianca
authors.initials: B
authors.lastname: Bruzzone
authors.forename: Claudio
authors.initials: C
authors.lastname: Viscoli
authors.forename: Gilberto
authors.initials: G
authors.lastname: Filaci
authors.forename: Antonio
authors.initials: A
authors.lastname: Di Biagio
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002490:Central Nervous System / Q000821:virology*; D003430:Cross-Sectional Studies; D005260:Female; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007558:Italy; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D008875:Middle Aged; D012367:RNA, Viral / Q000097:blood / Q000134:cerebrospinal fluid; D014766:Viremia
chemical_list: D015415:Biomarkers; D008562:Membrane Glycoproteins; D012367:RNA, Viral; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1
keywords: CD38,; Cerebrospinal fluid; HIV,; T lymphocytes,