title: Association of Elevations of Specific T Cell and Monocyte Subpopulations in Rheumatoid Arthritis With Subclinical Coronary Artery Atherosclerosis.
abstract: OBJECTIVE
  Coronary artery disease (CAD) is the leading cause of excess deaths in rheumatoid arthritis (RA). However, identification of features denoting patients with a risk of developing CAD is lacking. The composition of circulating peripheral blood mononuclear cell (PBMC) subsets in RA patients differs markedly from that in healthy controls with regard to the extent of T cell activation, with clonal expansion and differentiation to effector memory status, and presence of inflammatory monocytes. In this study, we sought to evaluate whether elevations in these PBMC subpopulations in RA patients could denote those with an increased risk of subclinical CAD, as determined by the presence of coronary artery calcification (CAC).
  
  
  METHODS
  The study cohort comprised 72 patients with RA who underwent cardiac computed tomography to assess CAC. PBMC subsets were determined by multiparameter flow cytometry. Multivariable logistic regression was used to determine the associations between PBMC subpopulations and the presence of CAC.
  
  
  RESULTS
  Among the 72 patients with RA, 33% had CAC and exhibited significant increases in the levels of circulating CD4 T cell subsets denoting activation and differentiation to the effector memory phenotypes. Analogous increases in the levels of CD8 T cell subsets, as well as in the CD14(high)CD16+ intermediate monocyte subset, were also present in these patients, as compared to those without CAC. The increases in the CD4 and CD8 T cell subsets were highly intercorrelated, whereas the increases in CD14(high)CD16+ monocytes were independent of elevations in the CD4 T cell subsets. After adjustments for relevant confounders, the levels of CD4+CD56+CD57+ T cells and CD14(high)CD16+ monocytes remained associated with the presence of CAC.
  
  
  CONCLUSIONS
  These findings indicate that PBMC subsets are markers for the presence of CAC and suggest that mechanisms of atherogenesis in RA may operate in part through the elevations in these subsets, raising further questions about the mechanisms underlying the presence of such alterations in cell composition in patients with RA and the potential for shared etiologic pathways between RA and cardiovascular disease.
authors.affiliation: Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Robert
authors.initials: R
authors.lastname: Winchester
authors.affiliation: Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Jon T
authors.initials: JT
authors.lastname: Giles
authors.affiliation: Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Simona
authors.initials: S
authors.lastname: Nativ
authors.affiliation: Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Kendall
authors.initials: K
authors.lastname: Downer
authors.affiliation: Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Hui-Zhu
authors.initials: HZ
authors.lastname: Zhang
authors.affiliation: Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Ayse
authors.initials: A
authors.lastname: Bag-Ozbek
authors.affiliation: Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Afshin
authors.initials: A
authors.lastname: Zartoshti
authors.affiliation: Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Sabahat
authors.initials: S
authors.lastname: Bokhari
authors.affiliation: Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: Joan M
authors.initials: JM
authors.lastname: Bathon
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D058070:Asymptomatic Diseases; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D019002:CD56 Antigen / Q000276:immunology; D018959:CD57 Antigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015331:Cohort Studies; D003324:Coronary Artery Disease / Q000000981:diagnostic imaging / Q000276:immunology*; D003430:Cross-Sectional Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D015999:Multivariate Analysis; D017452:Receptors, IgG / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014057:Tomography, X-Ray Computed; D061205:Vascular Calcification / Q000000981:diagnostic imaging / Q000276:immunology*
chemical_list: D019002:CD56 Antigen; D018959:CD57 Antigens; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG