title: The ADAR1 editing enzyme is encapsidated into HIV-1 virions.
abstract: Adenosine deaminase acting on RNA1 (ADAR1) was previously reported to affect HIV-1 replication. We report data showing that ADAR1 interacts with the HIV-1 p55 Gag protein, the major structural protein of the immature virus capsid. Furthermore, we found that the endogenous ADAR1 is incorporated into virions purified from the supernatant of primary HIV-1-infected CD4(+) T lymphocytes. Additional experiments demonstrated that the expression of the p55 Gag protein is sufficient for ADAR1 incorporation into virus-like particles (VLPs). Overall, our data originally support the evidence that ADAR1 can be part of the cell protein array uploaded in HIV-1 particles.
authors.affiliation: Department of Biomedicine and Prevention, University of Rome "Tor Vergata", Via Montpellier 1, 00133 Rome, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Orecchini
authors.affiliation: National AIDS Center, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Federico
authors.affiliation: Laboratory of Immunoinfectivology, Bambino Gesù Children׳s Hospital, IRCCS, Piazza S. Onofrio 4, 00165 Rome, Italy.
authors.forename: Margherita
authors.initials: M
authors.lastname: Doria
authors.affiliation: National AIDS Center, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Arenaccio
authors.affiliation: Laboratory of Immunoinfectivology, Bambino Gesù Children׳s Hospital, IRCCS, Piazza S. Onofrio 4, 00165 Rome, Italy.
authors.forename: Erica
authors.initials: E
authors.lastname: Giuliani
authors.affiliation: Department of Biomedicine and Prevention, University of Rome "Tor Vergata", Via Montpellier 1, 00133 Rome, Italy.
authors.forename: Silvia Anna
authors.initials: SA
authors.lastname: Ciafrè
authors.affiliation: Department of Biomedicine and Prevention, University of Rome "Tor Vergata", Via Montpellier 1, 00133 Rome, Italy. Electronic address: alessandro.michienzi@uniroma2.it.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Michienzi
mesh_terms: D000243:Adenosine Deaminase / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D014771:Virion; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000378:metabolism
chemical_list: D016601:RNA-Binding Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C000595356:ADAR protein, human; D000243:Adenosine Deaminase
keywords: ADAR1; Encapsidation; HIV-1; p55 Gag