title: Scanning color optical tomography (SCOT).
abstract: We have developed an interferometric optical microscope that provides three-dimensional refractive index map of a specimen by scanning the color of three illumination beams. Our design of the interferometer allows for simultaneous measurement of the scattered fields (both amplitude and phase) of such a complex input beam. By obviating the need for mechanical scanning of the illumination beam or detection objective lens; the proposed method can increase the speed of the optical tomography by orders of magnitude. We demonstrate our method using polystyrene beads of known refractive index value and live cells.
authors.forename: Poorya
authors.initials: P
authors.lastname: Hosseini
authors.forename: Yongjin
authors.initials: Y
authors.lastname: Sung
authors.forename: Youngwoon
authors.initials: Y
authors.lastname: Choi
authors.forename: Niyom
authors.initials: N
authors.lastname: Lue
authors.forename: Zahid
authors.initials: Z
authors.lastname: Yaqoob
authors.forename: Peter
authors.initials: P
authors.lastname: So
mesh_terms: D000818:Animals; D003116:Color; D005583:Fourier Analysis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D021621:Imaging, Three-Dimensional; D008853:Microscopy; D012031:Refractometry; D041622:Tomography, Optical / Q000379:methods*