title: Novel miRNA-31 and miRNA-200a-Mediated Regulation of Retinoblastoma Proliferation.
abstract: Retinoblastoma is the most common intraocular tumor in children. Current management includes broad-based treatments such as chemotherapy, enucleation, laser therapy, or cryotherapy. However, therapies that target specific pathways important for retinoblastoma progression could provide valuable alternatives for treatment. MicroRNAs are short, noncoding RNA transcripts that can regulate the expression of target genes, and their aberrant expression often facilitates disease. The identification of post-transcriptional events that occur after the initiating genetic lesions could further define the rapidly aggressive growth displayed by retinoblastoma tumors. In this study, we used two phenotypically different retinoblastoma cell lines to elucidate the roles of miRNA-31 and miRNA-200a in tumor proliferation. Our approach confirmed that miRNAs-31 and -200a expression is significantly reduced in human retinoblastomas. Moreover, overexpression of these two miRNAs restricts the expansion of a highly proliferative cell line (Y79), but does not restrict the growth rate of a less aggressive cell line (Weri1). Gene expression profiling of miRNA-31 and/or miRNA-200a-overexpressing cells identified differentially expressed mRNAs associated with the divergent response of the two cell lines. This work has the potential to enhance the development of targeted therapeutic approaches for retinoblastoma and improve the efficacy of treatment.
authors.affiliation: Human Molecular Genetics Program, Lurie Children's Research Center, Chicago, Illinois, 60611, United States of America; Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, Illinois, 60611, United States of America.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Montoya
authors.affiliation: Human Molecular Genetics Program, Lurie Children's Research Center, Chicago, Illinois, 60611, United States of America.
authors.forename: Hanli
authors.initials: H
authors.lastname: Fan
authors.affiliation: Department of Ophthalmology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, 60611, United States of America; Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, 60611, United States of America.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Bryar
authors.affiliation: Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, Illinois, 60611, United States of America; Division of Hematology, Oncology, and Stem Cell Transplantation, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, Illinois, 60611, United States of America.
authors.forename: Joanna L
authors.initials: JL
authors.lastname: Weinstein
authors.affiliation: Department of Ophthalmology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, 60611, United States of America; Division of Ophthalmology, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, Illinois, 60611, United States of America.
authors.forename: Marilyn B
authors.initials: MB
authors.lastname: Mets
authors.affiliation: Clinical & Translational Sciences Institute, Northwestern University Feinberg School of Medicine, Chicago, Illinois, 60611, United States of America.
authors.forename: Gang
authors.initials: G
authors.lastname: Feng
authors.affiliation: Human Molecular Genetics Program, Lurie Children's Research Center, Chicago, Illinois, 60611, United States of America.
authors.forename: Joshua
authors.initials: J
authors.lastname: Martin
authors.affiliation: Division of Hematology, Oncology, and Stem Cell Transplantation, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, Illinois, 60611, United States of America.
authors.forename: Alissa
authors.initials: A
authors.lastname: Martin
authors.affiliation: Department of Statistics, Weinberg College of Arts & Sciences, Northwestern University, Evanston, Illinois, 60208, United States of America.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Human Molecular Genetics Program, Lurie Children's Research Center, Chicago, Illinois, 60611, United States of America; Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, Illinois, 60611, United States of America; Division of Hematology, Oncology, and Stem Cell Transplantation, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, Illinois, 60611, United States of America.
authors.forename: Nikia A
authors.initials: NA
authors.lastname: Laurie
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D019572:Retinal Neoplasms / Q000235:genetics*; D012175:Retinoblastoma / Q000235:genetics*
chemical_list: D035683:MicroRNAs