title: Inhibition of Cell Growth of the Prostate Cancer Cell Model LNCaP by Cold Atmospheric Plasma.
abstract: Backround/Aim: Physical plasmas are ionized gases containing several biologically-reactive factors that yet exert their anti-microbial and anti-proliferative effects in fields of surface sterilisation, de-contamination and wound healing.
  
  
  METHODS
  Cold atmospheric plasma (CAP) was generated via the atmospheric pressure plasma jet kINPen09. Apoptotic effects of CAP treatment on the human epithelial prostate cancer cell line LNCaP as a cell culture model for malignant tumor tissue was analyzed by cell counting, western blot and quantitative reverse transcription-polymerase chain reaction analysis.
  
  
  RESULTS
  LNCaP cells exhibited significantly reduced cell growth following CAP treatment. We show that most probably the induction of apoptosis is the terminus of CAP treatment illustrated by the pro-apoptotic modulation of p53, p21, caspase-3, Bax, and survivin, as well as morphological changes of cell architecture.
  
  
  CONCLUSIONS
  Our in vitro study offers first indicatory results for molecular response mechanisms after CAP treatment in a suitable LNCaP cell model.
authors.affiliation: Department of Urology, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Weiss
authors.affiliation: Department of Trauma and Orthopedic Surgery, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Denis
authors.initials: D
authors.lastname: Gümbel
authors.affiliation: Department of Trauma and Orthopedic Surgery, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Gelbrich
authors.affiliation: Department of Anatomy and Cell Biology, RWTH Aachen Universtity, Aachen, Germany.
authors.forename: Lars-Ove
authors.initials: LO
authors.lastname: Brandenburg
authors.affiliation: Department of Urology, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Mandelkow
authors.affiliation: Department of Urology, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Zimmermann
authors.affiliation: Institute for Occupational and Social Medicine, RWTH Aachen Universtity, Aachen, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Ziegler
authors.affiliation: Department of Urology, University Medicine Greifswald, Greifswald, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Burchardt
authors.affiliation: Department of Urology, University Medicine Greifswald, Greifswald, Germany matthias.stope@uni-greifswald.de.
authors.forename: Matthias B
authors.initials: MB
authors.lastname: Stope
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D008297:Male; D058626:Plasma Gases / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000378:metabolism
chemical_list: D058626:Plasma Gases
keywords: Prostate cancer; apoptosis; cold atmospheric plasma; proliferation