title: Matrix metalloproteinase 13-containing exosomes promote nasopharyngeal carcinoma metastasis.
abstract: Nasopharyngeal cancer (NPC) is an endemic type of head and neck cancer with a high rate of cervical lymph node metastasis. Metastasis is the major cause of death in NPC patients. Increasing evidence indicates that exosomes play a pivotal role in promoting cancer metastasis by enhancing angiogenesis and ECM degradation. Matrix metalloproteinase 13 is an important kind of matrix proteinase that is often overexpressed in various tumors and increases the risk of metastasis. However, little is known about the potential role of MMP13-containing exosomes in NPC. In this study, we found that MMP13 was overexpressed in NPC cells and exosomes purified from conditioned medium (CM) as well as NPC patients' plasma. Transwell analysis revealed that MMP13-containing exosomes facilitated the metastasis of NPC cells. Furthermore, siRNA inhibited the effect of MMP13-containing exosomes on tumor cells metastasis as well as angiogenesis. The current findings provided novel insight into the vital role of MMP13-containing exosomes in NPC progression which might offer unique insights for potential therapeutic strategies for NPC progressions.
authors.affiliation: Department of Otorhinolaryngology, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: You
authors.affiliation: Department of Otorhinolaryngology, Medical School of Nantong University, Nantong, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shan
authors.affiliation: Department of Otorhinolaryngology, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Otorhinolaryngology, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Yue
authors.affiliation: Department of Otorhinolaryngology, Medical School of Nantong University, Nantong, China.
authors.forename: Bo
authors.initials: B
authors.lastname: You
authors.affiliation: Department of Otorhinolaryngology, Medical School of Nantong University, Nantong, China.
authors.forename: Si
authors.initials: S
authors.lastname: Shi
authors.affiliation: Department of Pathology, Medical School of Nantong University, Nantong, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pathology, Medical School of Nantong University, Nantong, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Cao
mesh_terms: D015153:Blotting, Western; D002277:Carcinoma; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D055354:Exosomes / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009389:Neovascularization, Pathologic; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D059016:Tumor Microenvironment / Q000502:physiology
chemical_list: D034741:RNA, Small Interfering; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13
keywords: Exosomes; MMP13; metastasis; microenvironment nasopharyngeal carcinoma