title: High-density interspecific genetic maps of kiwifruit and the identification of sex-specific markers.
abstract: Kiwifruit (Actinidia chinensis Planchon) is an important specialty fruit crop that suffers from narrow genetic diversity stemming from recent global commercialization and limited cultivar improvement. Here, we present high-density RAD-seq-based genetic maps using an interspecific F1 cross between Actinidia rufa 'MT570001' and A. chinensis 'Guihai No4'. The A. rufa (maternal) map consists of 2,426 single-nucleotide polymorphism (SNP) markers with a total length of 2,651 cM in 29 linkage groups (LGs) corresponding to the 29 chromosomes. The A. chinensis (paternal) map consists of 4,214 SNP markers over 3,142 cM in 29 LGs. Using these maps, we were able to anchor an additional 440 scaffolds from the kiwifruit draft genome assembly. Kiwifruit is functionally dioecious, which presents unique challenges for breeding and production. Three sex-specific simple sequence repeats (SSR) markers can be used to accurately sex type male and female kiwifruit in breeding programmes. The sex-determination region (SDR) in kiwifruit was narrowed to a 1-Mb subtelomeric region on chromosome 25. Localizing the SDR will expedite the discovery of genes controlling carpel abortion in males and pollen sterility in females.
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Liu
authors.affiliation: South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Donald Danforth Plant Science Center, St Louis, MO, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: VanBuren
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yao
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
authors.forename: Caihong
authors.initials: C
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China huanghw@scbg.ac.cn.
authors.forename: Hongwen
authors.initials: H
authors.lastname: Huang
mesh_terms: D029042:Actinidia / Q000235:genetics*; D002874:Chromosome Mapping; D005638:Fruit / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D005838:Genotype; D018895:Microsatellite Repeats; D000069600:Plant Breeding; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers
keywords: Actinidia chinensis; Actinidia rufa; RAD-seq; genome assembly; sex determination