title: Dye-linked D-amino acid dehydrogenases: biochemical characteristics and applications in biotechnology.
abstract: Dye-linked D-amino acid dehydrogenases (Dye-DADHs) catalyze the dehydrogenation of free D-amino acids in the presence of an artificial electron acceptor. Although Dye-DADHs functioning in catabolism of L-alanine and as primary enzymes in electron transport chains are widely distributed in mesophilic Gram-negative bacteria, biochemical and biotechnological information on these enzymes remains scanty. This is in large part due to their instability after isolation. On the other hand, in the last decade, several novel types of Dye-DADH have been found in thermophilic bacteria and hyperthermophilic archaea, where they contribute not only to L-alanine catabolism but also to the catabolism of other amino acids, including D-arginine and L-hydroxyproline. In this minireview, we summarize recent developments in our understanding of the biochemical characteristics of Dye-DADHs and their specific application to electrochemical biosensors.
authors.affiliation: Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui, 910-8507, Japan. satomura@u-fukui.ac.jp.
authors.forename: Takenori
authors.initials: T
authors.lastname: Satomura
authors.affiliation: Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan.
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sakuraba
authors.affiliation: Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui, 910-8507, Japan.|Department of Frontier Fiber Technology and Sciences, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui, 910-8507, Japan.
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Suye
authors.affiliation: Department of Biomedical engineering, Faculty of Engineering, Osaka Institute of Technology, Ohmiya, 5-16-1 Asahi-ku, Ohsaka, 535-8585, Japan.
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Ohshima
mesh_terms: D000409:Alanine / Q000378:metabolism; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis / Q000378:metabolism*; D001105:Archaea / Q000201:enzymology*; D015374:Biosensing Techniques; D001709:Biotechnology / Q000379:methods*; D003605:D-Amino-Acid Oxidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D006090:Gram-Negative Bacteria / Q000201:enzymology*; D012995:Solubility; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D003605:D-Amino-Acid Oxidase; D000409:Alanine
keywords: Amino acid catabolism; D-Amino acid dehydrogenase; Dye-linked dehydrogenase; Electrochemical biosensor; Flavoenzyme