title: Low level laser therapy reduces acute lung inflammation without impairing lung function.
abstract: Acute lung injury is a condition characterized by exacerbate inflammatory reaction in distal airways and lung dysfunction. Here we investigate the treatment of acute lung injury (ALI) by low level laser therapy (LLLT), an effective therapy used for the treatment of patients with inflammatory disorders or traumatic injuries, due to its ability to reduce inflammation and promote tissue regeneration. However, studies in internal viscera remains unclear. C57BL/6 mice were treated with intratracheal lipopolysaccharide (LPS) (5 mg/kg) or phosphate buffer saline (PBS). Six hours after instillation, two groups were irradiated with laser at 660 nm and radiant exposure of 10 J/cm2. Intratracheal LPS inoculation induced a marked increase in the number of inflammatory cells in perivascular and alveolar spaces. There was also an increase in the expression and secretion of cytokines (TNF-α, IL-1β, IL-6,) and chemokine (MCP-1). The LLLT application induced a significant decrease in both inflammatory cells influx and inflammatory mediators secretion. These effects did not affect lung mechanical properties, since no change was observed in tissue resistance or elastance. In conclusion LLLT is able to reduce inflammatory reaction in lungs exposed to LPS without affecting the pulmonary function and recovery.
authors.affiliation: University of São Paulo, School of Medicine, Emergency Medicine Department, Av. Dr. Arnaldo, 455, Sala 3189, São Paulo - SP, Brazil.
authors.forename: Vivian
authors.initials: V
authors.lastname: Cury
authors.affiliation: University of São Paulo, School of Medicine, Emergency Medicine Department, Av. Dr. Arnaldo, 455, Sala 3189, São Paulo - SP, Brazil.
authors.forename: Thais Martins
authors.initials: TM
authors.lastname: de Lima
authors.affiliation: Federal University of Sao Paulo, Biological Science Department, São Paulo - SP, Brazil.
authors.forename: Carla Maximo
authors.initials: CM
authors.lastname: Prado
authors.affiliation: University of São Paulo, School of Medicine, Emergency Medicine Department, Av. Dr. Arnaldo, 455, Sala 3189, São Paulo - SP, Brazil.
authors.forename: Nathalia
authors.initials: N
authors.lastname: Pinheiro
authors.affiliation: University of São Paulo, School of Medicine, Emergency Medicine Department, Av. Dr. Arnaldo, 455, Sala 3189, São Paulo - SP, Brazil.
authors.forename: Suely K K
authors.initials: SK
authors.lastname: Ariga
authors.affiliation: University of São Paulo, School of Medicine, Emergency Medicine Department, Av. Dr. Arnaldo, 455, Sala 3189, São Paulo - SP, Brazil.
authors.forename: Denise F
authors.initials: DF
authors.lastname: Barbeiro
authors.affiliation: University of São Paulo, Department of Cardio-Pulmonary, São Paulo - SP, Brazil.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Moretti
authors.affiliation: University of São Paulo, School of Medicine, Emergency Medicine Department, Av. Dr. Arnaldo, 455, Sala 3189, São Paulo - SP, Brazil.
authors.forename: Heraldo P
authors.initials: HP
authors.lastname: Souza
mesh_terms: D055371:Acute Lung Injury / Q000532:radiotherapy*; D000818:Animals; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D007249:Inflammation / Q000532:radiotherapy*; D028022:Low-Level Light Therapy; D008168:Lung / Q000503:physiopathology / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D018925:Chemokines; D016207:Cytokines
keywords: acute lung injury; low level laser therapy; lung function; photobiomodulation