title: Anti-inflammatory effect of desoxo-narchinol-A isolated from Nardostachys jatamansi against lipopolysaccharide.
abstract: We previously reported that Nardostachys jatamansi (NJ) exhibits anti-inflammatory activity against lipopolysaccharide (LPS). However, the active compound in NJ is unknown. Therefore, here, we examined the effects of desoxo-narchinol-A (DN) isolated from NJ against LPS-induced inflammation. To demonstrate the anti-inflammatory effect of DN against LPS, we used two models; murine endotoxin shock model for in vivo model, and peritoneal macrophage responses for in vitro. In endotoxin shock model, DN was administrated intraperitoneally 1h before LPS challenge, then we evaluated mice survival rates and organ damages. Pretreatment with DN (0.05mg/kg, 0.1mg/kg, or 0.5mg/kg) dramatically reduced mortality in a murine LPS-induced endotoxin shock model. Furthermore, DN inhibited tissue injury and production of pro-inflammatory cytokines, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor alpha (TNF-α), in the liver and lung. In in vitro macrophage model, we examined the inflammatory mediators and regulatory mechanisms such as mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB). DN inhibited the production of inflammatory mediators, such as inducible nitric oxide synthase (iNOS) and its derivative nitric oxide (NO), cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2), IL-1β, IL-6 and TNF-α and H3 protein acetylation in murine peritoneal macrophages. DN also inhibited p38 activation, but not extracellular signal-regulated kinase (ERK), c-jun NH2-terminal kinase (JNK), and NF-κB. These results suggest that DN from NJ exhibits protective effects against LPS-induced endotoxin shock and inflammation through p38 deactivation.
authors.affiliation: Hanbang Body Fluid Research Center, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea.
authors.forename: Joon Yeon
authors.initials: JY
authors.lastname: Shin
authors.affiliation: Hanbang Body Fluid Research Center, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea; Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea.
authors.forename: Gi-Sang
authors.initials: GS
authors.lastname: Bae
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea; BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea.
authors.forename: Sun-Bok
authors.initials: SB
authors.lastname: Choi
authors.affiliation: Hanbang Body Fluid Research Center, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea; Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea.
authors.forename: Il-Joo
authors.initials: IJ
authors.lastname: Jo
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea; BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea.
authors.forename: Dong-Goo
authors.initials: DG
authors.lastname: Kim
authors.affiliation: Department of Biomedical Chemistry, College of Health and Biomedical Science, Konkuk University, Chung-Ju 380-701, South Korea.
authors.forename: Dong-Sung
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Yanbian University, Ministry of Education, Yanji 133002, Jilin, China.
authors.forename: Ren-Bo
authors.initials: RB
authors.lastname: An
authors.affiliation: Hanbang Body Fluid Research Center, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea; College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, South Korea.
authors.forename: Hyuncheol
authors.initials: H
authors.lastname: Oh
authors.affiliation: Hanbang Body Fluid Research Center, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea; College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, South Korea.
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
authors.affiliation: Major in Integrated Oriental Medical Bioscience, Semyung University, Jecheon, Chungbuk 390-711, South Korea.
authors.forename: Yong Kook
authors.initials: YK
authors.lastname: Shin
authors.affiliation: Department of Pathology, College of Korean Medicine, Dongshin University, Naju, Jeonnam 520-714, South Korea.
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Jeong
authors.affiliation: Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea; BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea.
authors.forename: Ho-Joon
authors.initials: HJ
authors.lastname: Song
authors.affiliation: Hanbang Body Fluid Research Center, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea; Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea; BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, South Korea. Electronic address: parksj08@wku.ac.kr.
authors.forename: Sung-Joo
authors.initials: SJ
authors.lastname: Park
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis; D004789:Enzyme Activation / Q000187:drug effects; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000633:toxicity; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000187:drug effects; D009284:Naphthols / Q000302:isolation & purification / Q000494:pharmacology*; D032404:Nardostachys / Q000737:chemistry*; D012772:Shock, Septic / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D048051:p38 Mitogen-Activated Protein Kinases / Q000187:drug effects
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009284:Naphthols; C000610761:desoxonarchinol A; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Desoxo-narchinol A (DN); Endotoxin shock; Inflammation; Lipopolysaccharide (LPS); Nardostachys jatamansi (NJ)