title: Structural and functional evolution of 2',3'-cyclic nucleotide 3'-phosphodiesterase.
abstract: 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) is an abundant membrane-associated enzyme within the vertebrate myelin sheath. While the physiological function of CNPase still remains to be characterized in detail, it is known - in addition to its in vitro enzymatic activity - to interact with other proteins, small molecules, and membrane surfaces. From an evolutionary point of view, it can be deduced that CNPase is not restricted to myelin-forming cells or vertebrate tissues. Its evolution has involved gene fusion, addition of other small segments with distinct functions, such as membrane attachment, and possibly loss of function at the polynucleotide kinase-like domain. Currently, it is unclear whether the enzymatic function of the conserved phosphodiesterase domain in vertebrate myelin has a physiological role, or if CNPase could actually function - like many other classical myelin proteins - in a more structural role. This article is part of a Special Issue entitled SI: Myelin Evolution.
authors.affiliation: Faculty of Biochemistry and Molecular Medicine & Biocenter Oulu, University of Oulu, Aapistie 7, 90220 Oulu, Finland.
authors.forename: Matti
authors.initials: M
authors.lastname: Myllykoski
authors.affiliation: Centre for Bioinformatics, University of Hamburg, Bundesstraße 43, 20146 Hamburg, Germany.
authors.forename: Leonie
authors.initials: L
authors.lastname: Seidel
authors.affiliation: German Electron Synchrotron, Notkestraße 85, 22607 Hamburg, Germany.
authors.forename: Gopinath
authors.initials: G
authors.lastname: Muruganandam
authors.affiliation: Faculty of Biochemistry and Molecular Medicine & Biocenter Oulu, University of Oulu, Aapistie 7, 90220 Oulu, Finland; Department of Biomedicine, University of Bergen, Jonas Lies vei 91, 5009 Bergen, Norway.
authors.forename: Arne
authors.initials: A
authors.lastname: Raasakka
authors.affiliation: Centre for Bioinformatics, University of Hamburg, Bundesstraße 43, 20146 Hamburg, Germany.
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Torda
authors.affiliation: Faculty of Biochemistry and Molecular Medicine & Biocenter Oulu, University of Oulu, Aapistie 7, 90220 Oulu, Finland; German Electron Synchrotron, Notkestraße 85, 22607 Hamburg, Germany; Department of Biomedicine, University of Bergen, Jonas Lies vei 91, 5009 Bergen, Norway. Electronic address: petri.kursula@uib.no.
authors.forename: Petri
authors.initials: P
authors.lastname: Kursula
mesh_terms: D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D005075:Biological Evolution; D006801:Humans
chemical_list: D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
keywords: Enzyme; Evolution; Myelin protein; Protein domain; Protein structure; Sequence conservation