title: Identification of novel alternative splicing transcript and expression analysis of bovine TMEM95 gene.
abstract: Transmembrane protein 95 (TMEM95) is closely related to male reproductive performance in cattle, but does not affect semen quality. Alternative splicing plays an important role in regulating biological function as well as in generating proteomic and functional diversity in metazoan organisms. Thus, the aim of this study was to clone and identify transcripts of the TMEM95 gene in cattle using RT-PCR, characterize them via bioinformatics analysis, and detect their expression patterns using qRT-PCR. Two transcripts of TMEM95 were identified in cattle, including TMEM95-SV1 and TMEM95-SV2. Bioinformatics predicted that TMEM95-SV1 has a leucine-rich repeat C-terminal domain and a Pfam: IZUMO. These regions are closely related to protein interactions and the acrosome reaction, respectively. Interestingly, the two transcripts were exclusively expressed in the testes and brain in male fetus cattle, and TMEM95-SV1 was expressed in the brain at significantly higher levels than in the testis (P<0.05, 4.06-fold) and TMEM95-SV2 in the brain (P<0.05, 4.95-fold). These findings enrich the understanding of the TMEM95 gene function and benefit for enhancing male reproduction in cattle industry.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, PR China.
authors.forename: Sihuan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, PR China.
authors.forename: Hanfang
authors.initials: H
authors.lastname: Cai
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Shi
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, PR China.
authors.forename: Chuanying
authors.initials: C
authors.lastname: Pan
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, PR China.
authors.forename: Chuzhao
authors.initials: C
authors.lastname: Lei
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, PR China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Dang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, PR China. Electronic address: lanxianyong79@nwsuaf.edu.cn.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
mesh_terms: D000177:Acrosome / Q000378:metabolism; D017398:Alternative Splicing; D000818:Animals; D001921:Brain / Q000196:embryology* / Q000378:metabolism; D002417:Cattle; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D009928:Organ Specificity; D010802:Phylogeny; D059368:RNA Isoforms / Q000378:metabolism*; D013737:Testis / Q000196:embryology* / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D059368:RNA Isoforms
keywords: Cattle; MRNA expression; Reproduction; TMEM95 (transmembrane protein 95 gene); Transcript