title: Molecular evidence that the opportunistic fungal pathogen Trichosporon asahii is part of the normal fungal microbiota of the human gut based on rRNA genotyping.
abstract: The fungus Trichosporon asahii is distributed widely in the environment and is frequently isolated from clinical specimens. It can cause life-threatening disseminated infections, including breakthrough infection after the administration of candin antifungal drugs. However, the definitive infection route is still unclear. In this study, 43 T. asahii genotypes were detected in 72 fecal samples from healthy subjects. The rRNA genotypes of the species were determined using a culture-independent method. The genotypes of T. asahii were almost identical to those of reported clinical isolates. Therefore, T. asahii colonizing the gastrointestinal tract might be associated with the development of trichosporonosis, and not environmental isolates.
authors.affiliation: Department of Microbiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
authors.forename: Otomi
authors.initials: O
authors.lastname: Cho
authors.affiliation: Department of Microbiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
authors.forename: Manami
authors.initials: M
authors.lastname: Matsukura
authors.affiliation: Department of Microbiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan. Electronic address: sugita@my-pharm.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugita
mesh_terms: D000328:Adult; D021903:DNA, Ribosomal Spacer; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D008297:Male; D064307:Microbiota; D014250:Trichosporon / Q000235:genetics* / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D021903:DNA, Ribosomal Spacer
keywords: Genotype; Gut; Normal microbiota; Trichosporon; rRNA