title: Platelet-rich plasma gel composited with nondegradable porous polyurethane scaffolds as a potential auricular cartilage alternative.
abstract: Total auricular reconstruction is still a challenge, and autologous cartilage transplant is the main therapy so far. Tissue engineering provides a promising method for auricular cartilage reconstruction. However, although degradable framework demonstrated excellent initial cosmetic details, it is difficult to maintain the auricular contour over time and the metabolites tended to be harmful to human body. In this study, biocompatible and safe nondegradable elastic polyurethane was used to make porous scaffold in specific details by rapid prototyping technology. Platelet-rich plasma contains fibrin and abundant autologous growth factors, which was used as cell carriers for in vitro expanded cells. When crosslinking polyurethane framework, platelet-rich plasma and cells together, we successfully made polyurethane/platelet-rich plasma/cell composites, and implanted them into dorsal subcutaneous space of nude mice. The results showed that this method resulted in more even cell distribution and higher cell density, promoted chondrocyte proliferation, induced higher level expressions of aggrecan and type II collagen gene, increased content of newly developed glycosaminoglycans, and produced high-quality cartilaginous tissue. This kind of cartilage tissue engineering approach may be a potential promising alternative for external ear reconstruction.
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an, PR China.
authors.forename: Zhongshan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, PR China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an, PR China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an, PR China zhaoymdentist@163.com.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002358:Cartilage, Articular / Q000502:physiology; D049109:Cell Proliferation; D019902:Chondrocytes / Q000166:cytology; D024043:Collagen Type II / Q000737:chemistry; D004423:Ear / Q000502:physiology; D004425:Ear Cartilage / Q000502:physiology*; D005109:Extracellular Matrix / Q000737:chemistry; D005337:Fibrin / Q000737:chemistry; D005786:Gene Expression Regulation; D006025:Glycosaminoglycans / Q000737:chemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry; D051379:Mice; D008819:Mice, Nude; D010976:Platelet Count; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*; D016062:Porosity; D011817:Rabbits; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D024043:Collagen Type II; D006025:Glycosaminoglycans; D036341:Intercellular Signaling Peptides and Proteins; D011140:Polyurethanes; D005337:Fibrin
keywords: Platelet-rich plasma gel; auricular reconstruction; cartilage regeneration; extracellular matrix; polyurethane