title: Analysis of immune responses to recombinant proteins from strains of Mycoplasma mycoides subsp. mycoides, the causative agent of contagious bovine pleuropneumonia.
abstract: Current contagious bovine pleuropneumonia (CBPP) vaccines are based on live-attenuated strains of Mycoplasma mycoides subsp. mycoides (Mmm). These vaccines have shortcomings in terms of efficacy, duration of immunity and in some cases show severe side effects at the inoculation site; hence the need to develop new vaccines to combat the disease. Reverse vaccinology approaches were used and identified 66 candidate Mycoplasma proteins using available Mmm genome data. These proteins were ranked by their ability to be recognized by serum from CBPP-positive cattle and thereafter used to inoculate naïve cattle. We report here the inoculation of cattle with recombinant proteins and the subsequent humoral and T-cell-mediated immune responses to these proteins and conclude that a subset of these proteins are candidate molecules for recombinant protein-based subunit vaccines for CBPP control.
authors.affiliation: Vaccine Infectious Disease Organization - International Vaccine Centre (VIDO-InterVac), 120 Veterinary Rd, Saskatoon, SK S7N 5E3, Canada. Electronic address: jose.perez-casal@usask.ca.
authors.forename: Jose
authors.initials: J
authors.lastname: Perez-Casal
authors.affiliation: Vaccine Infectious Disease Organization - International Vaccine Centre (VIDO-InterVac), 120 Veterinary Rd, Saskatoon, SK S7N 5E3, Canada.
authors.forename: Tracy
authors.initials: T
authors.lastname: Prysliak
authors.affiliation: Vaccine Infectious Disease Organization - International Vaccine Centre (VIDO-InterVac), 120 Veterinary Rd, Saskatoon, SK S7N 5E3, Canada.
authors.forename: Teresa
authors.initials: T
authors.lastname: Maina
authors.affiliation: Vaccine Infectious Disease Organization - International Vaccine Centre (VIDO-InterVac), 120 Veterinary Rd, Saskatoon, SK S7N 5E3, Canada.
authors.forename: Yejun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Vaccine Infectious Disease Organization - International Vaccine Centre (VIDO-InterVac), 120 Veterinary Rd, Saskatoon, SK S7N 5E3, Canada.
authors.forename: Hugh
authors.initials: H
authors.lastname: Townsend
authors.affiliation: Vaccine Infectious Disease Organization - International Vaccine Centre (VIDO-InterVac), 120 Veterinary Rd, Saskatoon, SK S7N 5E3, Canada.
authors.forename: Emil
authors.initials: E
authors.lastname: Berverov
authors.affiliation: Kenya Agricultural and Livestock Research Organisation (KALRO), Kaptagat Rd, Loresho, P.O. Box 57811, Nairobi, Kenya.
authors.forename: Isabel
authors.initials: I
authors.lastname: Nkando
authors.affiliation: Kenya Agricultural and Livestock Research Organisation (KALRO), Kaptagat Rd, Loresho, P.O. Box 57811, Nairobi, Kenya.
authors.forename: Hezron
authors.initials: H
authors.lastname: Wesonga
authors.affiliation: International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi 00100, Kenya.
authors.forename: Anne
authors.initials: A
authors.lastname: Liljander
authors.affiliation: International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi 00100, Kenya.
authors.forename: Joerg
authors.initials: J
authors.lastname: Jores
authors.affiliation: International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi 00100, Kenya.
authors.forename: Jan
authors.initials: J
authors.lastname: Naessens
authors.affiliation: Vaccine Infectious Disease Organization - International Vaccine Centre (VIDO-InterVac), 120 Veterinary Rd, Saskatoon, SK S7N 5E3, Canada.
authors.forename: Volker
authors.initials: V
authors.lastname: Gerdts
authors.affiliation: Vaccine Infectious Disease Organization - International Vaccine Centre (VIDO-InterVac), 120 Veterinary Rd, Saskatoon, SK S7N 5E3, Canada.
authors.forename: Andrew
authors.initials: A
authors.lastname: Potter
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000097:blood; D009176:Mycoplasma mycoides / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D011002:Pleuropneumonia, Contagious / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D022223:Vaccines, Subunit / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic
keywords: CBPP; Humoral immune responses; Mycoplasma mycoides; Recombinant proteins