title: [Expression of Wnt5a in chronic apical periodontitis and its clinical significance].
abstract: OBJECTIVE
  To detect the expression of Wnt5a in lesions of chronic apical periodontitis and determine the relationship between expression of Wnt5a and inflammation degree.
  
  
  METHODS
  Ten patients with chronic apical periodontitis and 5 healthy controls were enrolled in this study. According to the inflammatory cell infiltration, the specimens were divided into 2 groups: severe inflammation group and mild inflammation group. The expression of Wnt5a was measured by real-time PCR (RT-PCR) and immunohistochemical analysis in the lesions of chronic apical periodontitis. The amount of Wnt5 expression was assayed and compared in different inflammation levels. Statistical analysis was performed using SPSS 13.0 software package.
  
  
  RESULTS
  Wnt5a was detected in both groups. Expression of Wnt5a mRNA in patients were significantly higher than the controls (P<0.05). According to inflammation level, the positive expression rate of Wnt5a in severe inflammation group was significantly higher than the controls (P<0.01), and Wnt5a positive expression in mild inflammation group was also significantly higher than the controls (P<0.05). The expression of Wnt5a was significantly different between severe inflammation group and mild inflammation group (P<0.05).
  
  
  CONCLUSIONS
  The expression of Wnt5a increases as the severity of tissue inflammation increases, which indicates that Wnt5a plays an important role in the development of chronic apical periodontitis.
authors.affiliation: Department of Endodontics, School of Stomatology, China Medical University;Lab of Endodontics, Liaoning Provincial Institute of Stomatology; Liaoning Provincial Research Center of Translational Oral Medicine. Shenyang 110002, Liaoning Province, China. E-mail:syria176820022@sina.com.
authors.forename: Li-ya
authors.initials: LY
authors.lastname: Xu
authors.forename: Nan
authors.initials: N
authors.lastname: Ma
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Qiu
authors.forename: Ya-qiong
authors.initials: YQ
authors.lastname: Yu
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
authors.forename: Jing-tao
authors.initials: JT
authors.lastname: Yu
authors.forename: Ming
authors.initials: M
authors.lastname: Xue
mesh_terms: D006801:Humans; D007249:Inflammation; D010485:Periapical Periodontitis / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D051153:Wnt Proteins / Q000378:metabolism*; D000071818:Wnt-5a Protein
chemical_list: D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein