title: Hypertrophic and antihypertrophic microRNA levels in peripheral blood mononuclear cells and their relationship to left ventricular hypertrophy in patients with essential hypertension.
abstract: MicroRNAs regulate several aspects of physiological and pathologic cardiac hypertrophy, and they represent promising therapeutic targets in cardiovascular disease. We assessed the expression levels of the microRNAs miR-1, miR-133a, miR-26b, miR-208b, miR-499, and miR-21, in 102 patients with essential hypertension and 30 healthy individuals. All patients underwent two-dimensional echocardiography. MicroRNA expression levels in peripheral blood mononuclear cells were quantified by real-time reverse transcription polymerase chain reaction. Hypertensive patients showed significantly lower miR-133a (5.06 ± 0.50 vs. 13.20 ± 2.15, P < .001) and miR-26b (6.76 ± 0.53 vs. 9.36 ± 1.40, P = .037) and higher miR-1 (25.99 ± 3.07 vs. 12.28 ± 2.06, P = .019), miR-208b (22.29 ± 2.96 vs. 8.73 ± 1.59, P = .016), miR-499 (10.06 ± 1.05 vs. 5.70 ± 0.91, P = .033), and miR-21 (2.75 ± 0.15 vs. 1.82 ± 0.20, P = .002) expression levels compared with healthy controls. In hypertensive patients, we observed significant negative correlations of miR-1 (r = -0.374, P < .001) and miR-133a (r = -0.431, P < .001) and significant positive correlations of miR-26b (r = 0.302, P = .002), miR-208b (r = 0.426, P < .001), miR-499 (r = 0.433, P < .001) and miR-21 (r = 0.498, P < .001) expression levels with left ventricular mass index. Our data reveal that miR-1, miR-133a, miR-26b, miR-208b, miR-499, and miR-21 show distinct expression profiles in hypertensive patients relative to healthy individuals and they are associated with clinical indices of left ventricular hypertrophy in hypertensive patients. Thus, they may be related to heart hypertrophy in hypertensive patients and are possibly candidate therapeutic targets in hypertensive heart disease.
authors.affiliation: Molecular Cardiology Laboratory, Department of Cardiology, School of Medicine, University of Crete, Heraklion, Greece. Electronic address: kontarak@med.uoc.gr.
authors.forename: Joanna E
authors.initials: JE
authors.lastname: Kontaraki
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Crete, Greece.
authors.forename: Maria E
authors.initials: ME
authors.lastname: Marketou
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Crete, Greece.
authors.forename: Fragiskos I
authors.initials: FI
authors.lastname: Parthenakis
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Crete, Greece.
authors.forename: Spyros
authors.initials: S
authors.lastname: Maragkoudakis
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Crete, Greece.
authors.forename: Evangelos A
authors.initials: EA
authors.lastname: Zacharis
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Crete, Greece.
authors.forename: Stelios
authors.initials: S
authors.lastname: Petousis
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Crete, Greece.
authors.forename: George E
authors.initials: GE
authors.lastname: Kochiadakis
authors.affiliation: Department of Cardiology, Heraklion University Hospital, Crete, Greece.
authors.forename: Panos E
authors.initials: PE
authors.lastname: Vardas
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood; D000075222:Essential Hypertension; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000150:complications; D017379:Hypertrophy, Left Ventricular / Q000097:blood* / Q000000981:diagnostic imaging / Q000209:etiology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014463:Ultrasonography
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; C544547:MIRN1 microRNA, human; C548234:MIRN133 microRNA, human; C550906:MIRN208 microRNA, human; C524940:MIRN21 microRNA, human; C528163:MIRN26 microRNA, human; C548683:MIRN499 microRNA, human
keywords: Left ventricular mass index; miR-1; miR-133a; miR-208b; miR-21; miR-26b; miR-499