title: Autoantibody response to Sui1 and its tissue-specific expression in hepatocellular carcinoma.
abstract: To investigate the immunogenicity of Homo sapiens putative translation initiation factor (Sui1) in hepatocellular carcinoma (HCC), enzyme-linked immunosorbent assay (ELISA) and Western blot were utilized to assess autoantibody responses to Sui1 in sera from HCC patients and healthy individuals. Indirect immunofluorescence (IIF) assay with cancer cells and immunohistochemistry (IHC) study with tissue array slides were performed to examine Sui1 expression profile in cancer cells and tissues. The data confirmed that the frequency of autoantibody to Sui1 in sera of HCC patients was 15.5 % (16/103), which was remarkably higher than that in sera of liver cirrhosis (LC) patients (3.3 %, 1/30), chronic hepatitis (CH) patients (0 %, 0/29), and normal human serum (NHS) (0 %, 0/82) (p < 0.01). IHC study showed that the Sui1 expression in HCC tissues was 26.7 % (16/60). The expression of Sui1 had the trend of increasing along with the cancer grades but no statistical significance (p > 0.05). In immunodiagnosis of HCC, the sensitivity and specificity of the anti-Sui1 antibody were 15.5 and 99.3 %, respectively. If both anti-Sui1 and alpha fetal protein (AFP) were simultaneously utilized as detective markers, 66.7 % (30/45) of HCC patients could be correctly distinguished. The results suggested that anti-Sui1 could be utilized as a supplementary serological marker for the detection of HCC and Sui1 might be associated to HCC carcinogenesis.
authors.affiliation: Department of Oncology, People's Hospital of Zhengzhou University (Henan Provincial People's Hospital), Zhengzhou, China.|Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX, 79968, USA.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Zhou
authors.affiliation: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX, 79968, USA.|Department of Stomatology, Zhengzhou First People's Hospital, Zhengzhou, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX, 79968, USA.|Surgical Department, People's Hospital of Zhengzhou University (Henan Provincial People's Hospital), Zhengzhou, China.
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Yue
authors.affiliation: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX, 79968, USA.
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Luo
authors.affiliation: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX, 79968, USA.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX, 79968, USA. jzhang@utep.edu.
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000276:immunology / Q000378:metabolism; D001323:Autoantibodies / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000378:metabolism; D063646:Carcinogenesis / Q000378:metabolism / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D039642:Eukaryotic Initiation Factors / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007159:Immunologic Tests / Q000379:methods; D008103:Liver Cirrhosis / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000276:immunology* / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000276:immunology* / Q000378:metabolism*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor; C410108:EIF1 protein, human; D039642:Eukaryotic Initiation Factors; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins
keywords: Autoantibody; Hepatocellular carcinoma; Immunoreactivity; Sui1; Tumor-associated antigen