title: Development of an in vitro Ovary Culture System to Evaluate Endocrine Disruption in Wood Frog Tadpoles.
abstract: Gonad-mesonephros complexes from wood frog (Lithobates sylvaticus) tadpoles were incubated in vitro for 1 wk to examine direct effects of naphthenic acids (NA) and 17α-ethinylestradiol (EE2) on ovarian differentiation. Histological images reveal successful preservation of gonadal integrity where cultured oocytes exhibited no significant differences in diameters and areas compared to uncultured conditions. Ovaries exposed to 10 μg/L 17α-EE2 contained oocytes with significantly advanced atresia and diminished areas and diameters, indicating a degree of ovarian regression. A significant reduction in oocyte area was observed in ovaries exposed to 3 mg/L of a commercial extract of petroleum-derived NA. This novel approach has applications for screening direct effects of endocrine-disrupting chemicals on gonadal function in tadpoles.
authors.affiliation: a Center for Advanced Research in Environmental Genomics, Department of Biology , University of Ottawa , Ottawa , Ontario , Canada.
authors.forename: Maria
authors.initials: M
authors.lastname: Vu
authors.affiliation: a Center for Advanced Research in Environmental Genomics, Department of Biology , University of Ottawa , Ottawa , Ontario , Canada.
authors.forename: Laia
authors.initials: L
authors.lastname: Navarro-Martín
authors.affiliation: a Center for Advanced Research in Environmental Genomics, Department of Biology , University of Ottawa , Ottawa , Ontario , Canada.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Gutierrez-Villagomez
authors.affiliation: a Center for Advanced Research in Environmental Genomics, Department of Biology , University of Ottawa , Ottawa , Ontario , Canada.
authors.forename: Vance L
authors.initials: VL
authors.lastname: Trudeau
mesh_terms: D000818:Animals; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D066298:In Vitro Techniques / Q000379:methods*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D009864:Ontario; D010053:Ovary / Q000187:drug effects*; D011898:Ranidae / Q000254:growth & development / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D052244:Endocrine Disruptors; D014874:Water Pollutants, Chemical