title: Baseline susceptibility to alpha-cypermethrin in Lutzomyia longipalpis (Lutz & Neiva, 1912) from Lapinha Cave (Brazil).
abstract: BACKGROUND
  Given the increase in cases of visceral leishmaniasis in recent years, associated with the socio-economic impact of this disease, as well as the wide distribution of Lutzomyia longipalpis in Brazil and the likelihood that this vector may develop resistance to insecticides used for control, the Ministry of Health considers as crucial the creation of a network in order to study and monitor the resistance of this vector to insecticides used for control. In this sense, this study aimed: 1) to characterize the susceptibility of L. longipalpis from Lapinha Cave (Lagoa Santa, MG - Brazil) to Alfateck SC200 in field bioassays, and 2) to define the susceptibility baseline to alpha-cypermethrin in laboratory bioassays, checking the possibility of using it as susceptibility reference lineage (SRL).
  
  
  RESULTS
  The field bioassays revealed that the tested population was highly susceptible to alpha-cypermethrin in all time periods with high mortality (~100 %) in all treated surfaces before six months after spraying. In the laboratory bioassays, the studied population presented LD50, LD95 and LD99 to 0.78013, 10.5580 and 31.067 mg/m(2), respectively. The slope was 1.454121.
  
  
  CONCLUSIONS
  The studied population of L. longipalpis was considered as adequate for SRL according criterion recommended by Pan-American Health Organization and has proven susceptibility to tested insecticide in the field. One cannot rule out the possibility of finding populations of L. longipalpis more susceptible to alpha-cypermethrin; therefore, further research is necessary on other populations with potential use as a SRL.
authors.affiliation: Laboratório de Referência em Triatomíneos e Epidemiologia da Doença de Chagas, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil. grasielle@cpqrr.fiocruz.br.
authors.forename: Grasielle Caldas Davila
authors.initials: GC
authors.lastname: Pessoa
authors.affiliation: Laboratório de Leishmanioses, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima 1715, Barro Preto, 29190-002, Belo Horizonte, MG, Brazil. josiane@cpqrr.fiocruz.br.
authors.forename: Josiane Valadão
authors.initials: JV
authors.lastname: Lopes
authors.affiliation: Centro de Controle de Zoonoses de Montes Claros, Montes Claros, MG, Brazil. rfmarilia@hotmail.com.
authors.forename: Marília Fonseca
authors.initials: MF
authors.lastname: Rocha
authors.affiliation: Laboratório de Referência em Triatomíneos e Epidemiologia da Doença de Chagas, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil. leticia.pinheiro@cpqrr.fiocruz.br.
authors.forename: Letícia C
authors.initials: LC
authors.lastname: Pinheiro
authors.affiliation: Laboratório de Referência em Triatomíneos e Epidemiologia da Doença de Chagas, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil. aline.rosa@cpqrr.fiocruz.br.
authors.forename: Aline Cristine Luiz
authors.initials: AC
authors.lastname: Rosa
authors.affiliation: Laboratório de Leishmanioses, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima 1715, Barro Preto, 29190-002, Belo Horizonte, MG, Brazil. erika@cpqrr.fiocruz.br.
authors.forename: Érika Monteiro
authors.initials: ÉM
authors.lastname: Michalsky
authors.affiliation: Laboratório de Leishmanioses, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima 1715, Barro Preto, 29190-002, Belo Horizonte, MG, Brazil. edel@cpqrr.fiocruz.br.
authors.forename: Edelberto Santos
authors.initials: ES
authors.lastname: Dias
mesh_terms: D000818:Animals; D001681:Biological Assay; D001938:Brazil; D007306:Insecticides / Q000494:pharmacology*; D007928:Lethal Dose 50; D011576:Psychodidae / Q000187:drug effects* / Q000502:physiology*; D011722:Pyrethrins / Q000494:pharmacology*; D016019:Survival Analysis
chemical_list: D007306:Insecticides; D011722:Pyrethrins; C017160:cypermethrin