title: DNA Damage in Vicia faba by Exposure to Agricultural Soils from Tlaxcala, Mexico.
abstract: The aim of this research was to quantify some POPs, such as p,p' DDT, p,p' DDE, and PCBs in agricultural soils of Tlaxcala, Mexico and evaluate their capacity for eliciting DNA damage, using Vicia faba as bioindicator. The values of ΣDDTs and ΣPCBs ranged from 8-24 to 118-26,983 µg/kg, respectively. The samples T1 (HQ = 9.3) and T2 (HQ = 53.9) showed concentrations of ΣPCBs higher than Canadian guidelines (SQGE = 500 µg/kg). The genotoxicity testing produced percentages of DNA fragmentation higher than negative control and statistically significant (p < 0.05), both in agricultural soils and organic extracts. The soils T2, T3, N4, and N5 showed a DICA from 2.6 to 3.1 times, statistically higher (p < 0.05) than negative control. In general, the agricultural soils have greater genotoxic capacity than the organic extracts, suggesting a potential risk to biota that depends upon this ecosystem.
authors.affiliation: Centro de Investigación en Genética y Ambiente, Universidad Autónoma de Tlaxcala, Autopista San Martín-Tlaxcala Km. 10.5, 90120, Ixtacuixtla, Tlaxcala, Mexico. liber_05@yahoo.com.mx.
authors.forename: L
authors.initials: L
authors.lastname: Juárez-Santacruz
authors.affiliation: Centro de Investigación en Genética y Ambiente, Universidad Autónoma de Tlaxcala, Autopista San Martín-Tlaxcala Km. 10.5, 90120, Ixtacuixtla, Tlaxcala, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: García-Nieto
authors.affiliation: Centro de Investigación en Genética y Ambiente, Universidad Autónoma de Tlaxcala, Autopista San Martín-Tlaxcala Km. 10.5, 90120, Ixtacuixtla, Tlaxcala, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: García-Gallegos
authors.affiliation: Laboratorio de Ciencias Ambientales, Área Académica de Química, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km 4.5, 42076, Mineral de la Reforma, Hidalgo, Mexico.
authors.forename: C
authors.initials: C
authors.lastname: Romo-Gómez
authors.affiliation: Facultad de Odontología, Universidad Autónoma de Tlaxcala, Av. Ribereña S/N, Col. Centro, 90000, Tlaxcala, Tlaxcala, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Ortiz-Ortiz
authors.affiliation: División Ciencias de la Vida, Departamento de Ingeniería Ambiental, Campus Irapuato-Salamanca, Universidad Autónoma de Guanajuato, Guanajuato, Mexico.
authors.forename: R
authors.initials: R
authors.lastname: Costilla-Salazar
authors.affiliation: Centro de Investigación en Cambio Climatico, Universidad Autónoma de Tlaxcala, Autopista San Martín-Tlaxcala Km. 10.5, 90120, Ixtacuixtla, Tlaxcala, Mexico.
authors.forename: H S
authors.initials: HS
authors.lastname: Luna-Zendejas
mesh_terms: D000383:Agriculture; D003634:DDT / Q000032:analysis / Q000633:toxicity*; D053938:DNA Fragmentation / Q000187:drug effects; D004784:Environmental Monitoring / Q000379:methods; D008800:Mexico; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000633:toxicity*; D012987:Soil; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*; D031307:Vicia faba / Q000187:drug effects*
chemical_list: D012987:Soil; D012989:Soil Pollutants; D003634:DDT; D011078:Polychlorinated Biphenyls
keywords: Agricultural soil; Comet assay; POPs; Vicia faba