title: Molecular species delimitation methods recover most song-delimited cicada species in the European Cicadetta montana complex.
abstract: Molecular species delimitation is increasingly being used to discover and illuminate species level diversity, and a number of methods have been developed. Here, we compare the ability of two molecular species delimitation methods to recover song-delimited species in the Cicadetta montana cryptic species complex throughout Europe. Recent bioacoustics studies of male calling songs (premating reproductive barriers) have revealed cryptic species diversity in this complex. Maximum likelihood and Bayesian phylogenetic analyses were used to analyse the mitochondrial genes COI and COII and the nuclear genes EF1α and period for thirteen European Cicadetta species as well as the closely related monotypic genus Euboeana. Two molecular species delimitation methods, general mixed Yule-coalescent (GMYC) and Bayesian phylogenetics and phylogeography, identified the majority of song-delimited species and were largely congruent with each other. None of the molecular delimitation methods were able to fully recover a recent radiation of four Greek species.
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Wade
authors.affiliation: Department of Environmental Sciences, Biogeography, University of Basel, Basel, Switzerland.
authors.forename: T
authors.initials: T
authors.lastname: Hertach
authors.affiliation: Slovenian Academy of Sciences and Arts, Ljubljana, Slovenia.
authors.forename: M
authors.initials: M
authors.lastname: Gogala
authors.affiliation: Slovenian Museum of Natural History, Ljubljana, Slovenia.
authors.forename: T
authors.initials: T
authors.lastname: Trilar
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT, USA.|School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
authors.forename: C
authors.initials: C
authors.lastname: Simon
mesh_terms: D000819:Animal Communication; D000818:Animals; D006430:Hemiptera / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny
keywords: European cicada; bioacoustics; cryptic species; gene tree/species tree; phylogenetics