title: The Mycobacterium tuberculosis PPE protein Rv1168c induces stronger B cell response than Rv0256c in active TB patients.
abstract: Tuberculosis (TB) caused by Mycobacterium tuberculosis is a serious global health problem and is responsible for millions of deaths every year. For effective control of this dreadful disease, it is necessary to diagnose TB cases at the initial stages of infection. The serodiagnosis of disease represents simple, rapid and inexpensive method that can be used at the primary health care levels. In this study we have compared sensitivity of two PPE proteins of M. tuberculosis, i.e., Rv0256c and Rv1168c for their use as serodiagnostic markers in active tuberculosis patients. Employing a standardized enzyme immunoassay with these PPE proteins as candidate antigens we were able to successfully discriminate the TB patients' sera from the BCG-vaccinated healthy controls. Further, we observed that Rv1168c displayed higher sensitivity in detecting extrapulmonary and smear negative pulmonary TB cases which are difficult to diagnose by available diagnostic methods. Overall the study highlights that Rv1168c can be used as a potential serodiagnostic marker for the diagnosis of active tuberculosis disease.
authors.affiliation: Laboratory of Molecular Cell Biology, Centre for DNA Fingerprinting and Diagnostics (CDFD), Gruhakalpa Building, Nampally, Hyderabad 500001, India. Electronic address: microphilip_14@rediffmail.com.
authors.forename: Philip Raj
authors.initials: PR
authors.lastname: Abraham
authors.affiliation: Laboratory of Molecular Cell Biology, Centre for DNA Fingerprinting and Diagnostics (CDFD), Gruhakalpa Building, Nampally, Hyderabad 500001, India; Graduate Studies, Manipal University, Manipal 576104, India. Electronic address: atul@cdfd.org.in.
authors.forename: Atul
authors.initials: A
authors.lastname: Udgata
authors.affiliation: Bhagwan Mahavir Medical Research Center, 10-1-1, Mahavir Marg, A.C. Guards, Hyderabad 500004, India. Electronic address: sumanlathag@yahoo.com.
authors.forename: Gaddam Suman
authors.initials: GS
authors.lastname: Latha
authors.affiliation: Laboratory of Molecular Cell Biology, Centre for DNA Fingerprinting and Diagnostics (CDFD), Gruhakalpa Building, Nampally, Hyderabad 500001, India. Electronic address: sangita@cdfd.org.in.
authors.forename: Sangita
authors.initials: S
authors.lastname: Mukhopadhyay
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001500:BCG Vaccine / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods / Q000592:standards; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D014376:Tuberculosis / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology* / Q000517:prevention & control; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001500:BCG Vaccine; D001426:Bacterial Proteins; C582018:PPE2 protein, Mycobacterium tuberculosis; C530985:Rv1168c protein, Mycobacterium tuberculosis
keywords: Enzyme immunoassay; Mycobacterium tuberculosis; Rv0256c; Rv1168c; Serodiagnosis