title: Differences in the Osteogenic Differentiation Capacity of Omental Adipose-Derived Stem Cells in Obese Patients With and Without Metabolic Syndrome.
abstract: Multiple studies have suggested that the reduced differentiation capacity of multipotent adipose tissue-derived mesenchymal stem cells (ASCs) in obese subjects could compromise their use in cell therapy. Our aim was to assess the osteogenic potential of omental ASCs and to examine the status of the isolated CD34(negative)-enriched fraction of omental-derived ASCs from subjects with different metabolic profiles. Omental ASCs from normal-weight subjects and subjects with or without metabolic syndrome were isolated, and the osteogenic potential of omental ASCs was evaluated. Additionally, osteogenic and clonogenic potential, proliferation rate, mRNA expression levels of proteins involved in redox balance, and fibrotic proteins were examined in the CD34(negative)-enriched fraction of omental-derived ASCs. Both the omental ASCs and the CD34(negative)-enriched fraction of omental ASCs from subjects without metabolic syndrome have a greater osteogenic potential than those from subjects with metabolic syndrome. The alkaline phosphatase and osteonectin mRNA were negatively correlated with nicotinamide adenine dinucleotide phosphate oxidase-2 mRNA and the mRNA expression levels of the fibrotic proteins correlated positively with nicotinamide adenine dinucleotide phosphate oxidase-5 mRNA and the homeostasis model assessment. Although the population doubling time was significantly higher in subjects with a body mass index of 25 kg/m(2) or greater, only the CD34(negative)-enriched omental ASC fraction in the subjects with metabolic syndrome had a higher population doubling time than the normal-weight subjects. The osteogenic, clonogenic, fibrotic potential, and proliferation rate observed in vitro suggest that omental ASCs from subjects without metabolic syndrome are more suitable for therapeutic osteogenic applications than those from subjects with metabolic syndrome.
authors.affiliation: Department of Clinical Endocrinology and Nutrition (W.O.-O., S.L., L.C.-A., J.A.T., R.E.B., F.J.T.), Institute of Biomedical Research of Malaga, Hospital Complex of Malaga (Virgen de la Victoria), University of Malaga, and Department of Orthopedic Surgery and Traumatology (A.L.G.), Virgen de la Victoria University Hospital and University of Malaga, and Department of Internal Medicine (M.R.B.-L.), Regional University Hospital of Malaga, Institute of Biomedical Research of Malaga, 29010 Malaga, Spain; Centro de Investigación Biomédica en Red of Obesity Physiopathology and Nutrition (W.O.-O., S.L., L..C.-A., J.A.T., M.R.B.-L., R.E.B., F.J.T.), and Centro de Investigación Biomédica en Red of Diabetes and Associated Metabolic Disorders (S.F.-V.), Institute of Health Salud Carlos III, 28029 Madrid, Spain; Department of Endocrinology and Nutrition (P.P.G.L.) and Unit of Innovation in Minimally Invasive Surgery (S.M.C.), Department of Surgery, Virgen del Rocío University Hospital, 41013 Seville, Spain; and University Hospital of Tarragona Joan XXIII Institut d'Investigació (S.F.-V.), Sanitaria Pere Virgili, Universitat Rovirai Virgili, 43003 Tarragona, Spain.
authors.forename: Wilfredo
authors.initials: W
authors.lastname: Oliva-Olivera
authors.affiliation: Department of Clinical Endocrinology and Nutrition (W.O.-O., S.L., L.C.-A., J.A.T., R.E.B., F.J.T.), Institute of Biomedical Research of Malaga, Hospital Complex of Malaga (Virgen de la Victoria), University of Malaga, and Department of Orthopedic Surgery and Traumatology (A.L.G.), Virgen de la Victoria University Hospital and University of Malaga, and Department of Internal Medicine (M.R.B.-L.), Regional University Hospital of Malaga, Institute of Biomedical Research of Malaga, 29010 Malaga, Spain; Centro de Investigación Biomédica en Red of Obesity Physiopathology and Nutrition (W.O.-O., S.L., L..C.-A., J.A.T., M.R.B.-L., R.E.B., F.J.T.), and Centro de Investigación Biomédica en Red of Diabetes and Associated Metabolic Disorders (S.F.-V.), Institute of Health Salud Carlos III, 28029 Madrid, Spain; Department of Endocrinology and Nutrition (P.P.G.L.) and Unit of Innovation in Minimally Invasive Surgery (S.M.C.), Department of Surgery, Virgen del Rocío University Hospital, 41013 Seville, Spain; and University Hospital of Tarragona Joan XXIII Institut d'Investigació (S.F.-V.), Sanitaria Pere Virgili, Universitat Rovirai Virgili, 43003 Tarragona, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Leiva Gea
authors.affiliation: Department of Clinical Endocrinology and Nutrition (W.O.-O., S.L., L.C.-A., J.A.T., R.E.B., F.J.T.), Institute of Biomedical Research of Malaga, Hospital Complex of Malaga (Virgen de la Victoria), University of Malaga, and Department of Orthopedic Surgery and Traumatology (A.L.G.), Virgen de la Victoria University Hospital and University of Malaga, and Department of Internal Medicine (M.R.B.-L.), Regional University Hospital of Malaga, Institute of Biomedical Research of Malaga, 29010 Malaga, Spain; Centro de Investigación Biomédica en Red of Obesity Physiopathology and Nutrition (W.O.-O., S.L., L..C.-A., J.A.T., M.R.B.-L., R.E.B., F.J.T.), and Centro de Investigación Biomédica en Red of Diabetes and Associated Metabolic Disorders (S.F.-V.), Institute of Health Salud Carlos III, 28029 Madrid, Spain; Department of Endocrinology and Nutrition (P.P.G.L.) and Unit of Innovation in Minimally Invasive Surgery (S.M.C.), Department of Surgery, Virgen del Rocío University Hospital, 41013 Seville, Spain; and University Hospital of Tarragona Joan XXIII Institut d'Investigació (S.F.-V.), Sanitaria Pere Virgili, Universitat Rovirai Virgili, 43003 Tarragona, Spain.
authors.forename: Said
authors.initials: S
authors.lastname: Lhamyani
authors.affiliation: Department of Clinical Endocrinology and Nutrition (W.O.-O., S.L., L.C.-A., J.A.T., R.E.B., F.J.T.), Institute of Biomedical Research of Malaga, Hospital Complex of Malaga (Virgen de la Victoria), University of Malaga, and Department of Orthopedic Surgery and Traumatology (A.L.G.), Virgen de la Victoria University Hospital and University of Malaga, and Department of Internal Medicine (M.R.B.-L.), Regional University Hospital of Malaga, Institute of Biomedical Research of Malaga, 29010 Malaga, Spain; Centro de Investigación Biomédica en Red of Obesity Physiopathology and Nutrition (W.O.-O., S.L., L..C.-A., J.A.T., M.R.B.-L., R.E.B., F.J.T.), and Centro de Investigación Biomédica en Red of Diabetes and Associated Metabolic Disorders (S.F.-V.), Institute of Health Salud Carlos III, 28029 Madrid, Spain; Department of Endocrinology and Nutrition (P.P.G.L.) and Unit of Innovation in Minimally Invasive Surgery (S.M.C.), Department of Surgery, Virgen del Rocío University Hospital, 41013 Seville, Spain; and University Hospital of Tarragona Joan XXIII Institut d'Investigació (S.F.-V.), Sanitaria Pere Virgili, Universitat Rovirai Virgili, 43003 Tarragona, Spain.
authors.forename: Leticia
authors.initials: L
authors.lastname: Coín-Aragüez
authors.affiliation: Department of Clinical Endocrinology and Nutrition (W.O.-O., S.L., L.C.-A., J.A.T., R.E.B., F.J.T.), Institute of Biomedical Research of Malaga, Hospital Complex of Malaga (Virgen de la Victoria), University of Malaga, and Department of Orthopedic Surgery and Traumatology (A.L.G.), Virgen de la Victoria University Hospital and University of Malaga, and Department of Internal Medicine (M.R.B.-L.), Regional University Hospital of Malaga, Institute of Biomedical Research of Malaga, 29010 Malaga, Spain; Centro de Investigación Biomédica en Red of Obesity Physiopathology and Nutrition (W.O.-O., S.L., L..C.-A., J.A.T., M.R.B.-L., R.E.B., F.J.T.), and Centro de Investigación Biomédica en Red of Diabetes and Associated Metabolic Disorders (S.F.-V.), Institute of Health Salud Carlos III, 28029 Madrid, Spain; Department of Endocrinology and Nutrition (P.P.G.L.) and Unit of Innovation in Minimally Invasive Surgery (S.M.C.), Department of Surgery, Virgen del Rocío University Hospital, 41013 Seville, Spain; and University Hospital of Tarragona Joan XXIII Institut d'Investigació (S.F.-V.), Sanitaria Pere Virgili, Universitat Rovirai Virgili, 43003 Tarragona, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Alcaide Torres
authors.affiliation: Department of Clinical Endocrinology and Nutrition (W.O.-O., S.L., L.C.-A., J.A.T., R.E.B., F.J.T.), Institute of Biomedical Research of Malaga, Hospital Complex of Malaga (Virgen de la Victoria), University of Malaga, and Department of Orthopedic Surgery and Traumatology (A.L.G.), Virgen de la Victoria University Hospital and University of Malaga, and Department of Internal Medicine (M.R.B.-L.), Regional University Hospital of Malaga, Institute of Biomedical Research of Malaga, 29010 Malaga, Spain; Centro de Investigación Biomédica en Red of Obesity Physiopathology and Nutrition (W.O.-O., S.L., L..C.-A., J.A.T., M.R.B.-L., R.E.B., F.J.T.), and Centro de Investigación Biomédica en Red of Diabetes and Associated Metabolic Disorders (S.F.-V.), Institute of Health Salud Carlos III, 28029 Madrid, Spain; Department of Endocrinology and Nutrition (P.P.G.L.) and Unit of Innovation in Minimally Invasive Surgery (S.M.C.), Department of Surgery, Virgen del Rocío University Hospital, 41013 Seville, Spain; and University Hospital of Tarragona Joan XXIII Institut d'Investigació (S.F.-V.), Sanitaria Pere Virgili, Universitat Rovirai Virgili, 43003 Tarragona, Spain.
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Bernal-López
authors.affiliation: Department of Clinical Endocrinology and Nutrition (W.O.-O., S.L., L.C.-A., J.A.T., R.E.B., F.J.T.), Institute of Biomedical Research of Malaga, Hospital Complex of Malaga (Virgen de la Victoria), University of Malaga, and Department of Orthopedic Surgery and Traumatology (A.L.G.), Virgen de la Victoria University Hospital and University of Malaga, and Department of Internal Medicine (M.R.B.-L.), Regional University Hospital of Malaga, Institute of Biomedical Research of Malaga, 29010 Malaga, Spain; Centro de Investigación Biomédica en Red of Obesity Physiopathology and Nutrition (W.O.-O., S.L., L..C.-A., J.A.T., M.R.B.-L., R.E.B., F.J.T.), and Centro de Investigación Biomédica en Red of Diabetes and Associated Metabolic Disorders (S.F.-V.), Institute of Health Salud Carlos III, 28029 Madrid, Spain; Department of Endocrinology and Nutrition (P.P.G.L.) and Unit of Innovation in Minimally Invasive Surgery (S.M.C.), Department of Surgery, Virgen del Rocío University Hospital, 41013 Seville, Spain; and University Hospital of Tarragona Joan XXIII Institut d'Investigació (S.F.-V.), Sanitaria Pere Virgili, Universitat Rovirai Virgili, 43003 Tarragona, Spain.
authors.forename: Pedro Pablo
authors.initials: PP
authors.lastname: García-Luna
authors.affiliation: Department of Clinical Endocrinology and Nutrition (W.O.-O., S.L., L.C.-A., J.A.T., R.E.B., F.J.T.), Institute of Biomedical Research of Malaga, Hospital Complex of Malaga (Virgen de la Victoria), University of Malaga, and Department of Orthopedic Surgery and Traumatology (A.L.G.), Virgen de la Victoria University Hospital and University of Malaga, and Department of Internal Medicine (M.R.B.-L.), Regional University Hospital of Malaga, Institute of Biomedical Research of Malaga, 29010 Malaga, Spain; Centro de Investigación Biomédica en Red of Obesity Physiopathology and Nutrition (W.O.-O., S.L., L..C.-A., J.A.T., M.R.B.-L., R.E.B., F.J.T.), and Centro de Investigación Biomédica en Red of Diabetes and Associated Metabolic Disorders (S.F.-V.), Institute of Health Salud Carlos III, 28029 Madrid, Spain; Department of Endocrinology and Nutrition (P.P.G.L.) and Unit of Innovation in Minimally Invasive Surgery (S.M.C.), Department of Surgery, Virgen del Rocío University Hospital, 41013 Seville, Spain; and University Hospital of Tarragona Joan XXIII Institut d'Investigació (S.F.-V.), Sanitaria Pere Virgili, Universitat Rovirai Virgili, 43003 Tarragona, Spain.
authors.forename: Salvador
authors.initials: S
authors.lastname: Morales Conde
authors.affiliation: Department of Clinical Endocrinology and Nutrition (W.O.-O., S.L., L.C.-A., J.A.T., R.E.B., F.J.T.), Institute of Biomedical Research of Malaga, Hospital Complex of Malaga (Virgen de la Victoria), University of Malaga, and Department of Orthopedic Surgery and Traumatology (A.L.G.), Virgen de la Victoria University Hospital and University of Malaga, and Department of Internal Medicine (M.R.B.-L.), Regional University Hospital of Malaga, Institute of Biomedical Research of Malaga, 29010 Malaga, Spain; Centro de Investigación Biomédica en Red of Obesity Physiopathology and Nutrition (W.O.-O., S.L., L..C.-A., J.A.T., M.R.B.-L., R.E.B., F.J.T.), and Centro de Investigación Biomédica en Red of Diabetes and Associated Metabolic Disorders (S.F.-V.), Institute of Health Salud Carlos III, 28029 Madrid, Spain; Department of Endocrinology and Nutrition (P.P.G.L.) and Unit of Innovation in Minimally Invasive Surgery (S.M.C.), Department of Surgery, Virgen del Rocío University Hospital, 41013 Seville, Spain; and University Hospital of Tarragona Joan XXIII Institut d'Investigació (S.F.-V.), Sanitaria Pere Virgili, Universitat Rovirai Virgili, 43003 Tarragona, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Fernández-Veledo
authors.affiliation: Department of Clinical Endocrinology and Nutrition (W.O.-O., S.L., L.C.-A., J.A.T., R.E.B., F.J.T.), Institute of Biomedical Research of Malaga, Hospital Complex of Malaga (Virgen de la Victoria), University of Malaga, and Department of Orthopedic Surgery and Traumatology (A.L.G.), Virgen de la Victoria University Hospital and University of Malaga, and Department of Internal Medicine (M.R.B.-L.), Regional University Hospital of Malaga, Institute of Biomedical Research of Malaga, 29010 Malaga, Spain; Centro de Investigación Biomédica en Red of Obesity Physiopathology and Nutrition (W.O.-O., S.L., L..C.-A., J.A.T., M.R.B.-L., R.E.B., F.J.T.), and Centro de Investigación Biomédica en Red of Diabetes and Associated Metabolic Disorders (S.F.-V.), Institute of Health Salud Carlos III, 28029 Madrid, Spain; Department of Endocrinology and Nutrition (P.P.G.L.) and Unit of Innovation in Minimally Invasive Surgery (S.M.C.), Department of Surgery, Virgen del Rocío University Hospital, 41013 Seville, Spain; and University Hospital of Tarragona Joan XXIII Institut d'Investigació (S.F.-V.), Sanitaria Pere Virgili, Universitat Rovirai Virgili, 43003 Tarragona, Spain.
authors.forename: Rajaa
authors.initials: R
authors.lastname: El Bekay
authors.affiliation: Department of Clinical Endocrinology and Nutrition (W.O.-O., S.L., L.C.-A., J.A.T., R.E.B., F.J.T.), Institute of Biomedical Research of Malaga, Hospital Complex of Malaga (Virgen de la Victoria), University of Malaga, and Department of Orthopedic Surgery and Traumatology (A.L.G.), Virgen de la Victoria University Hospital and University of Malaga, and Department of Internal Medicine (M.R.B.-L.), Regional University Hospital of Malaga, Institute of Biomedical Research of Malaga, 29010 Malaga, Spain; Centro de Investigación Biomédica en Red of Obesity Physiopathology and Nutrition (W.O.-O., S.L., L..C.-A., J.A.T., M.R.B.-L., R.E.B., F.J.T.), and Centro de Investigación Biomédica en Red of Diabetes and Associated Metabolic Disorders (S.F.-V.), Institute of Health Salud Carlos III, 28029 Madrid, Spain; Department of Endocrinology and Nutrition (P.P.G.L.) and Unit of Innovation in Minimally Invasive Surgery (S.M.C.), Department of Surgery, Virgen del Rocío University Hospital, 41013 Seville, Spain; and University Hospital of Tarragona Joan XXIII Institut d'Investigació (S.F.-V.), Sanitaria Pere Virgili, Universitat Rovirai Virgili, 43003 Tarragona, Spain.
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Tinahones
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D015992:Body Mass Index; D016022:Case-Control Studies; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D005355:Fibrosis / Q000235:genetics; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D024821:Metabolic Syndrome / Q000378:metabolism*; D008875:Middle Aged; D039902:Multipotent Stem Cells; D000074662:NADPH Oxidase 2; D000074664:NADPH Oxidase 5; D019255:NADPH Oxidases / Q000235:genetics; D009765:Obesity / Q000378:metabolism*; D009852:Omentum / Q000166:cytology; D010012:Osteogenesis / Q000235:genetics*; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D018952:Antigens, CD34; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D015676:Osteonectin; D012333:RNA, Messenger; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D000074664:NADPH Oxidase 5; D019255:NADPH Oxidases; C431571:NOX5 protein, human; D000469:Alkaline Phosphatase