title: Effectiveness of Coupled Application of AmpFℓSTR Yfiler Kit and Reduced Size Y-chromosomal Short Tandem Repeat Analysis for Archeological Human Bones.
abstract: The AmpFℓSTR Yfiler PCR Amplification (Yfiler) kit continues to be improved for a better analytical efficiency in cases of highly degraded DNA. The authors endeavored to determine whether coupling of the Yfiler kit with supplemental multiplex amplification of some Y-STR loci is a more efficient analytical mode for poorly preserved human femurs (n = 15) discovered at Korean archeological sites. To reveal locus profiles not easily obtained by Yfiler analysis, custom-designed primers were adopted for the DYS390, DYS391, DYS392, DYS438, DYS439, and DYS635 loci. The success rate for 16 Y-STR locus profiles obtained from the 15 femurs was improved from 18.33% (in the use of Yfiler kit only) to 49.17% (the coupled use of Yfiler and custom-designed primers). In this study, the authors established that the custom-designed primers offer a markedly improved success rate for obtainment of Y-STR profiles from degraded aDNA not easily identified by sole use of the Yfiler assay.
authors.affiliation: Institute of Forensic Science, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul, 110-799, South Korea.|Department of Anatomy, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul, 110-799, South Korea.
authors.forename: Chang Seok
authors.initials: CS
authors.lastname: Oh
authors.affiliation: Institute of Forensic Science, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul, 110-799, South Korea.|Department of Forensic Medicine, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul, 110-799, South Korea.
authors.forename: Soong Deok
authors.initials: SD
authors.lastname: Lee
authors.affiliation: Department of Forensic Medicine, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-Gu, Seoul, 120-752, South Korea.
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Shin
authors.affiliation: Institute of Forensic Science, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul, 110-799, South Korea.|Department of Anatomy, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul, 110-799, South Korea.
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Shin
mesh_terms: D041322:Chromosomes, Human, Y; D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005269:Femur / Q000737:chemistry*; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers
keywords: AmpFℓSTR Yfiler PCR amplification kit; Joseon; Korea; Y-chromosomal short tandem repeat; Y-miniplex primer; ancient DNA; forensic science