title: A recyclable biotransformation system for L-2-aminobutyric acid production based on immobilized enzyme technology.
abstract: OBJECTIVE
  To make the previously developed biosynthesis of L-2-aminobutyric acid (L-ABA) more suitable for the industrial-scale production.
  
  
  RESULTS
  A recyclable biotransformation system was developed based on immobilized enzyme technology. The conversion yield of L-threonine (at 90 g l(-1)) reached 99.9 % and the theoretical yield of L-ABA reached more than 90 % using the optimized biotransformation system by the individual immobilization of threonine deaminase and the co-immobilization of L leucine dehydrogenase and formate dehydrogenase. 90 g L-threonine l(-1) was converted to 73.9 g L-ABA l(-1) >95 % theoretical yield, within 120-145 min in 30 batch transformation experiments.
  
  
  CONCLUSIONS
  The recyclable biotransformation system is promising to fulfill industrial requirements for L-ABA production.
authors.affiliation: College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, 410004, China.|Hunan Flag Bio-tech Co. Ltd, Changsha, 410100, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.affiliation: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.|Shanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai, 200237, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Huzhou Research Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Huzhou, 313000, China.
authors.forename: Rongshen
authors.initials: R
authors.lastname: Tao
authors.affiliation: Hunan Flag Bio-tech Co. Ltd, Changsha, 410100, China.
authors.forename: Shengfeng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Hunan Flag Bio-tech Co. Ltd, Changsha, 410100, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China. syang@sibs.ac.cn.|Shanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai, 200237, China. syang@sibs.ac.cn.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
mesh_terms: D000613:Aminobutyrates / Q000378:metabolism*; D001711:Biotransformation; D004800:Enzymes, Immobilized; D017743:Equipment Reuse / Q000191:economics; D004926:Escherichia coli / Q000378:metabolism; D050803:Leucine Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D013913:Threonine Dehydratase / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000613:Aminobutyrates; D004800:Enzymes, Immobilized; C012223:alpha-aminobutyric acid; D050803:Leucine Dehydrogenase; D013913:Threonine Dehydratase
keywords: Deamination; Hydrogenation; Immobilization; L-2-Aminobutyric acid; L-Threonine