title: Changes in Surface Charge Density of Blood Cells in Fatal Accidental Hypothermia.
abstract: The objective of this research was to evaluate postmortem changes concerning electric charge of human erythrocytes and thrombocytes in fatal accidental hypothermia. The surface charge density values were determined on the basis of the electrophoretic mobility measurements of the cells conducted at various pH values of electrolyte solution. The surface charge of erythrocyte membranes after fatal accidental hypothermia increased compared to the control group within whole range of experimental pH values. Moreover, a slight shift of the isoelectric point of erythrocyte membranes towards high pH values was observed. The surface charge of thrombocyte membranes in fatal accidental hypothermia decreased at low pH compared to the control group. However, at pH range 4-9, the values increased compared to the control group. The isoelectric point of thrombocyte membranes after fatal accidental hypothermia was slightly shifted towards low pH values compared to the control group. The observed changes are probably connected with the partial destruction and functional changes of the blood cell structure.
authors.affiliation: Department of Forensic Medicine, Medical University of Bialystok, Waszyngtona St. 13, 15-230, Bialystok, Poland. michalszeremeta@gmail.com.
authors.forename: Michał
authors.initials: M
authors.lastname: Szeremeta
authors.affiliation: Institute of Chemistry, University of Bialystok, K. Ciolkowskiego St. 1K, 15-245, Bialystok, Poland.
authors.forename: Aneta Dorota
authors.initials: AD
authors.lastname: Petelska
authors.affiliation: Institute of Chemistry, University of Bialystok, K. Ciolkowskiego St. 1K, 15-245, Bialystok, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Kotyńska
authors.affiliation: Department of Forensic Medicine, Medical University of Bialystok, Waszyngtona St. 13, 15-230, Bialystok, Poland.
authors.forename: Witold
authors.initials: W
authors.lastname: Pepiński
authors.affiliation: Institute of Chemistry, University of Bialystok, K. Ciolkowskiego St. 1K, 15-245, Bialystok, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Naumowicz
authors.affiliation: Institute of Chemistry, University of Bialystok, K. Ciolkowskiego St. 1K, 15-245, Bialystok, Poland.|Laboratory of Electrochemical Power Sources, Faculty of Chemistry, University of Warsaw, Pasteur St. 1, 02-093, Warsaw, Poland.
authors.forename: Zbigniew Artur
authors.initials: ZA
authors.lastname: Figaszewski
authors.affiliation: Department of Forensic Medicine, Medical University of Bialystok, Waszyngtona St. 13, 15-230, Bialystok, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Niemcunowicz-Janica
mesh_terms: D000328:Adult; D000368:Aged; D001344:Autopsy; D001773:Blood Cells / Q000737:chemistry / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007035:Hypothermia / Q000097:blood / Q000378:metabolism*; D007526:Isoelectric Point; D008297:Male; D008875:Middle Aged; D055672:Static Electricity; D055815:Young Adult
keywords: Erythrocyte; Fatal accidental hypothermia; Surface charge density; Thrombocyte; pH measurement