title: The Acute Effect of Humic Acid on Iron Accumulation in Rats.
abstract: Free iron leads to the formation of pro-oxidant reactive oxygen species (ROS). Humic acids (HAs) enhance permeability of cellular wall and act as a chelator through electron transferring. This study was designed to test chelator effect of HA on iron as well as its anti-oxidant effect against the iron-induced hepatotoxicity and cardiotoxicity. The rats used were randomly divided into four groups (n = 8/group): group I (the control group); group II (the HA group), humic acid (562 mg/kg) was given over 10 days by oral gavage; group III (the iron group), iron III hydroxide polymaltose (250 mg/kg) was given over 10 days by intraperitoneal route; and group IV (the HA plus iron group), received the iron (similar to group II) plus humic acid (similar to those in groups II and III) group. Blood and two tissue samples both from liver and heart were obtained for biochemical and histopathological evaluations. Iron deposition, the iron-induced hepatotoxicity, and cardiotoxicity were demonstrated by histopathological and biochemical manner. However, no significant differences were observed in the serum biochemical values and the histopathological results among the iron and the HA plus iron groups in the liver tissue but not in the heart tissue. The protective effects of humic acid against iron-induced cardiotoxicity were shown but not against hepatotoxicity in our study.
authors.affiliation: Department of Gastroenterology, Medical Faculty, Inonu University, 44280, Malatya, Turkey. yasir.cagin@inonu.edu.tr.
authors.forename: Yasir Furkan
authors.initials: YF
authors.lastname: Cagin
authors.affiliation: Department of Pathology, Medical Faculty, Inonu University, Malatya, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Sahin
authors.affiliation: Department of Physiology, Medical Faculty, Inonu University, Malatya, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Polat
authors.affiliation: Department of Gastroenterology, Medical Faculty, Inonu University, 44280, Malatya, Turkey.
authors.forename: M A
authors.initials: MA
authors.lastname: Erdogan
authors.affiliation: Department of Gastroenterology, Medical Faculty, Inonu University, 44280, Malatya, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Atayan
authors.affiliation: Department of Pharmaceutical Toxicology, Inonu University, Malatya, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Eyol
authors.affiliation: Department of Gastroenterology, Medical Faculty, Inonu University, 44280, Malatya, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Bilgic
authors.affiliation: Department of Gastroenterology, Medical Faculty, Inonu University, 44280, Malatya, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Seckin
authors.affiliation: Department of Biostatistics and Medical Informatics, Medical Faculty, Inonu University, Malatya, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Colak
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D002614:Chelating Agents / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005290:Ferric Compounds / Q000008:administration & dosage / Q000097:blood / Q000633:toxicity; D006321:Heart / Q000187:drug effects*; D006812:Humic Substances; D007274:Injections, Intraperitoneal; D007501:Iron / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D005290:Ferric Compounds; D006812:Humic Substances; D017382:Reactive Oxygen Species; D007501:Iron; C013276:teferrol
keywords: Cardiotoxicity; Chelation; Hepatotoxicity; Humic acid; Iron