title: Review seed biopharmaceutical cyclic peptides: From discovery to applications.
abstract: Mini-proteins (or peptides) with disulfide bond/s and a cyclic backbone offer exciting opportunities for applications in medicine, as these ribosomally synthesized and posttranslationally modified peptides are exceptionally stable and amenable to grafting epitopes with desirable activities. Here I discuss important aspects of the discovery and applications of disulfide-bonded cyclic peptides from seeds, i.e., the trypsin inhibitor cyclotides and the preproalbumin with sunflower trypsin inhibitor-derived peptides, focusing on bioanalytical methods for and insights generated from their discovery as well as their potential use as engineering scaffolds for peptide-based drug design. The recent discovery of their precursors and processing enzymes could potentially enable in planta production of designer disulfide-bonded cyclic peptides, preferably in edible seeds, and address the demand for new biopharmaceutical peptides in a cost-effective manner.
authors.affiliation: Department of Agricultural Product Technology, Faculty of Agricultural Technology, Brawijaya University, Malang, East Java, 65145, Indonesia.
authors.forename: Tunjung
authors.initials: T
authors.lastname: Mahatmanto
mesh_terms: D000595:Amino Acid Sequence; D004220:Disulfides / Q000737:chemistry; D055808:Drug Discovery; D008969:Molecular Sequence Data; D029775:Momordica / Q000196:embryology; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
chemical_list: D004220:Disulfides; D010456:Peptides, Cyclic
keywords: Momordica; cyclic peptides; drug design; seeds; sunflower