title: Investigation of the Actual Causes of Hip Joint Implant Loosening Classified as Aseptic--Analysis of Microbiological Culture Results and Levels of Inflammatory Markers.
abstract: Loosening of the hip joint prosthesis is considered as one of the most significant postoperative complications in recent years. The laboratory diagnostic procedure used to differentiate periprosthetic infection from aseptic loosening is very difficult because of the biofilm which microorganisms form on the implant surface. The purpose of this research was to evaluate the level of concordance between clinical classification of implant loosening among 50 patients subjected to reimplantation procedure and laboratory investigation of PJI including microbiological culture results and the levels of inflammatory markers assessed in the patients' synovial fluid samples, serum, and full blood. The synovial fluid was collected for leukocyte count, differential cell count, and culture on standard media. The levels of systemic inflammation markers such as the ESR and CRP concentration were determined in serum and full blood. Tissue samples were collected for microbiological studies. Components from endoprostheses were exposed to ultrasound in a process called sonication. Among the parameters measured in serum and full blood the levels of ESR and CRP were higher in the septic group of patients. Cytologic analysis of synovial fluid was in correlation with microbiologic identification. The most frequent isolated bacteria was Staphylococcus epidermidis. Culture results from materials such as synovial fluid, sonicate and tissues are crucial to establish the infectious aetiology of the loosening. Microscopic analysis of synovial fluid represents a simple, rapid and accurate method for differentiating PJI from aseptic failure. Sonication increases detection of the infectious process, and culture results are in correlation with the cytologic analysis of synovial fluid.
authors.forename: Dagmara
authors.initials: D
authors.lastname: Strzelec-Nowak
authors.forename: Maria
authors.initials: M
authors.lastname: Kozioł-Montewka
authors.forename: Justyna
authors.initials: J
authors.lastname: Niedźwiadek
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Bogut
authors.forename: Jan
authors.initials: J
authors.lastname: Blacha
authors.forename: Tomasz
authors.initials: T
authors.lastname: Mazurkiewicz
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques; D015415:Biomarkers; D006622:Hip Prosthesis / Q000009:adverse effects*; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D007593:Joint Instability / Q000175:diagnosis; D008875:Middle Aged; D016459:Prosthesis-Related Infections / Q000175:diagnosis*; D013582:Synovial Fluid / Q000737:chemistry / Q000166:cytology / Q000382:microbiology
chemical_list: D015415:Biomarkers