title: Quantification of 16 polycyclic aromatic hydrocarbons in cigarette smoke condensate using stable isotope dilution liquid chromatography with atmospheric-pressure photoionization tandem mass spectrometry.
abstract: A stable isotope dilution liquid chromatography tandem mass spectrometry method for the analysis of 16 polycyclic aromatic hydrocarbons in cigarette smoke condensate was developed and validated. Compared with previously reported methods, this method has lower limits of detection (0.04-1.35 ng/cig). Additionally, the proposed method saves time, reduces the number of separation steps, and reduces the quantity of solvent needed. The new method was applied to evaluate polycyclic aromatic hydrocarbon content in 213 commercially available cigarettes in China, under the International Standardization Organization smoking regime and the Health Canadian intense smoking regime. The results showed that the total polycyclic aromatic hydrocarbon content was more than two times higher in samples from the Health Canadian intense smoking regime than in samples from the International Standardization Organization smoking regime (1189.23 versus 2859.50 ng/cig, p < 0.05). Meanwhile, the concentration of individual polycyclic aromatic hydrocarbons (and total polycyclic aromatic hydrocarbons) increased with labeled tar content in both of the tested smoking regimes. There was a positive correlation between total polycyclic aromatic hydrocarbons under the International Standardization Organization smoking regime with that under the Health Canadian intense smoking regime. The proposed liquid chromatography tandem mass spectrometry method is satisfactory for the rapid, sensitive, and accurate quantitative evaluation of polycyclic aromatic hydrocarbon content in cigarette smoke condensate, and it can be applied to assess potential health risks from smoking.
authors.affiliation: China National Tobacco Quality Supervision & Test Centre, Zhengzhou, China.|Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China.
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: China National Tobacco Quality Supervision & Test Centre, Zhengzhou, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Hou
authors.affiliation: China National Tobacco Quality Supervision & Test Centre, Zhengzhou, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Chen
authors.affiliation: Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China.
authors.forename: An
authors.initials: A
authors.lastname: Wang
authors.affiliation: China National Tobacco Quality Supervision & Test Centre, Zhengzhou, China.|Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China.
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Hu
keywords: Cigarette smoke condensate; Liquid chromatography tandem mass spectrometry; Polycyclic aromatic hydrocarbons; Smoking regimes