title: Expression of bioactive recombinant human fibroblast growth factor 10 in Carthamus tinctorius L. seeds.
abstract: Fibroblast growth factor 10 (FGF10) is a member of the FGF superfamily. It exhibits diverse biological functions, and is extensively used for fundamental research and clinical applications involving hair growth, tissue repair, and burn wounds. Oil bodies, obtained from oil seeds, have been exploited for a variety of biotechnology applications. The use of oil bodies reduces purification steps and costs associated with the production of heterogonous proteins. Here, recombinant human FGF10 (rhFGF10) was expressed in safflower (Carthamus tinctorius L.) seeds using oilbody-oleosin technology. A plant expression vector, pOTBar-oleosin-rhFGF10, was constructed and introduced into safflower using Agrobacterium tumefaciens transformation, and mature safflower plants were obtained by grafting. Oleosin-rhFGF10 was successfully transformed and expressed in safflower seeds and inherited to the T3generation. Moreover, MTT assays demonstrated that oil bodies expressed oleosin-FGF10 had a dose-dependent effect on cellular proliferation. In conclusion, this may provide a method of producing oleosin-rhFGF10, and help us meet the increasing pharmacological demands for the protein.
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China; College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, Jilin, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Guan
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China; College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, Jilin, China.
authors.forename: Shanyong
authors.initials: S
authors.lastname: Yi
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
authors.forename: Linna
authors.initials: L
authors.lastname: Du
authors.affiliation: School of Pharmaceutical Science, Wenzhou Medical College, Wenzhou 325035, Zhejiang, China.
authors.forename: Haishan
authors.initials: H
authors.lastname: Tian
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhai
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China; School of Pharmaceutical Science, Wenzhou Medical College, Wenzhou 325035, Zhejiang, China. Electronic address: xiaokunli@163.net.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China; School of Pharmaceutical Science, Wenzhou Medical College, Wenzhou 325035, Zhejiang, China. Electronic address: changjiang10@hotmail.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D031187:Carthamus tinctorius / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D051526:Fibroblast Growth Factor 10 / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D066292:Lipid Droplets / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: C496463:FGF10 protein, human; D051526:Fibroblast Growth Factor 10; D010940:Plant Proteins; D011994:Recombinant Proteins
keywords: Oil body; Oleosin; Safflower (Carthamus tinctorius L.); rhFGF10