title: Live four-dimensional optical coherence tomography reveals embryonic cardiac phenotype in mouse mutant.
abstract: Efficient phenotyping of developmental defects in model organisms is critical for understanding the genetic specification of normal development and congenital abnormalities in humans. We previously reported that optical coherence tomography (OCT) combined with live embryo culture is a valuable tool for mouse embryo imaging and four-dimensional (4-D) cardiodynamic analysis; however, its capability for analysis of mouse mutants with cardiac phenotypes has not been previously explored. Here, we report 4-D (three-dimensional+time) OCT imaging and analysis of the embryonic heart in a Wdr19 mouse mutant, revealing a heart looping defect. Quantitative analysis of cardiac looping revealed a statistically significant difference between mutant and control embryos. Our results indicate that live 4-D OCT imaging provides a powerful phenotyping approach to characterize embryonic cardiac function in mouse models.
authors.affiliation: Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston 77030, United States.
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Lopez
authors.affiliation: Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston 77030, United States.
authors.forename: Shang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston 77030, United StatesbUniversity of Houston, Department of Biomedical Engineering, 3605 Cullen Boulevard, Houston 77204, United StatescSamara State Aer.
authors.forename: Kirill V
authors.initials: KV
authors.lastname: Larin
authors.affiliation: Baylor College of Medicine, Department of Molecular & Cellular Biology, One Baylor Plaza, Houston 77030, United States.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Overbeek
authors.affiliation: Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston 77030, United States.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Larina
mesh_terms: D000818:Animals; D055424:Cardiac-Gated Imaging Techniques / Q000379:methods*; D004622:Embryo, Mammalian / Q000473:pathology*; D005315:Fetal Diseases; D006330:Heart Defects, Congenital / Q000196:embryology* / Q000473:pathology*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D008817:Mice, Mutant Strains; D011296:Prenatal Diagnosis / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique