title: Unraveling of cross talk between Ca(2+) and ROS regulating enzymes in Anabaena 7120 and ntcA mutant.
abstract: In order to understand a cross talk between Ca(2+) and ROS regulating enzymes and the possible involvement of ntcA gene, Anabaena sp. PCC 7120 and its derivative ntcA mutant grown in varied levels of calcium chloride (0, 1, 10, and 100 mM) have been investigated. Scanning Electron Microscopy showed abnormal structure formation at high calcium concentration (100 mM) both in wild type and mutant. Fv /Fm values suggested that 100 mM calcium concentration was detrimental for photosynthetic apparatus. SOD, catalase, APX, GR, and peroxidase activity were found to be maximum for 100 mM and minimum for 1 mM of exogenously supplied calcium salt. NADPH contents were higher for wild type than mutant. RAPD-PCR and SDS-PAGE analysis revealed a difference in DNA as well as proteome pattern with changes in calcium chloride regime. Prominent bands of approximately 70, 33, 21, and 14 kDa expressed in the wild type served as the marker polypeptide bands under calcium supplementation. Results suggest that higher levels of calcium ion disturb the cellular homeostasis generating ROS, thereby inducing enhanced levels of antioxidative enzymes. Further, data also suggests possible involvement of ntcA gene in cross talk between calcium ion and ROS regulating enzymes.
authors.affiliation: Department of Botany, Laboratory of Microbial Genetics, Banaras Hindu University, Varanasi, India.
authors.forename: Savita
authors.initials: S
authors.lastname: Singh
authors.affiliation: Department of Botany, Laboratory of Microbial Genetics, Banaras Hindu University, Varanasi, India.
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Mishra
mesh_terms: D017033:Anabaena / Q000201:enzymology* / Q000235:genetics; D060387:Ascorbate Peroxidases / Q000378:metabolism; D002122:Calcium Chloride / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial / Q000235:genetics; D005980:Glutathione Reductase / Q000378:metabolism; D051922:PII Nitrogen Regulatory Proteins / Q000235:genetics*; D009195:Peroxidase / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D051922:PII Nitrogen Regulatory Proteins; D017382:Reactive Oxygen Species; D014157:Transcription Factors; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D002122:Calcium Chloride
keywords: Calcium chloride; Cross talk; Cyanobacterium Anabaena 7120; ROS regulating enzymes; ntcA mutant