title: [Human papilloma virus genotyping in women with abnormal cytology].
abstract: OBJECTIVE
  To assess presence of HPV infection and identification of the most common HPV types in patients with abnormal cytology based on the Bethesda system (atypical squamous cells).
  
  
  METHODS
  81 women with abnormal cytology based on the Bethesda system (atypical squamous cells) were qualified for the study. Material was taken from the cervical canal, the vaginal portion of the cervix and the vagina onto a liquid medium to detect HPV DNA and genotyping of 19 most common oncogenic types of high and medium risk was performed with the Papillomastrip method and for HPV types 6 and 11 with the PCR method.
  
  
  RESULTS
  HPV was detected in 53 out of 81 examined women, which accounted for 66%. The most common HPV types were: 6/11 - 23 cases (43% of women with infection), 16 - 23 cases (43% of women with infection), 18 and 33 with 9 cases each (17% of women with infection). Coexistence of 6/11 with 16 or 18 - 13 concerned 15 patients (28% of women with infection) and presence of HPV 16 or 18 was detected in 28 cases (53% of women with infection). Positive HPV type contained in the quadrivalent vaccine against human papillomavirus 6/11 and 16 or 18 was detected in 38 patients (72% of women with infection). 40% of HPV positive women were infected with only one type of the virus, 26%--with two types and 23% with three types.
  
  
  CONCLUSIONS
  In 81 women with abnormal cytology based on the Bethesda system (atypical squamous cells) within 66% of HPV positive results the most common were type 6/11 (of low oncogenic potential but responsible for anogenital warts) and type 16 of high oncogenic potential.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Olejniczak
authors.forename: Dorota
authors.initials: D
authors.lastname: Rabiega-Gmyrek
authors.forename: Joanna
authors.initials: J
authors.lastname: Niepsuj-Biniaś
authors.forename: Paweł
authors.initials: P
authors.lastname: Jachowski
authors.forename: Bogna
authors.initials: B
authors.lastname: Guglas-Bochyńska
authors.forename: Anna
authors.initials: A
authors.lastname: Latos- Bieleńska
authors.forename: Jakub
authors.initials: J
authors.lastname: Woźniak
authors.forename: Tomasz
authors.initials: T
authors.lastname: Opala
mesh_terms: D000328:Adult; D065309:Atypical Squamous Cells of the Cervix; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology; D005260:Female; D060005:Genotyping Techniques; D052162:Human papillomavirus 16 / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000821:virology*; D014626:Vaginal Smears / Q000379:methods; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000473:pathology / Q000821:virology*