title: Coordination of self-renewal in glioblastoma by integration of adhesion and microRNA signaling.
abstract: Cancer stem cells (CSCs) provide an additional layer of complexity for tumor models and targets for therapeutic development. The balance between CSC self-renewal and differentiation is driven by niche components including adhesion, which is a hallmark of stemness. While studies have demonstrated that the reduction of adhesion molecules, such as integrins and junctional adhesion molecule-A (JAM-A), decreases CSC maintenance. The molecular circuitry underlying these interactions has yet to be resolved.
  
  
  
  MicroRNA screening predicted that microRNA-145 (miR-145) would bind to JAM-A. JAM-A overexpression in CSCs was evaluated both in vitro (proliferation and self-renewal) and in vivo (intracranial tumor initiation). miR-145 introduction into CSCs was similarly assessed in vitro. Additionally, The Cancer Genome Atlas dataset was evaluated for expression levels of miR-145 and overall survival of the different molecular groups.
  
  
  
  Using patient-derived glioblastoma CSCs, we confirmed that JAM-A is suppressed by miR-145. CSCs expressed low levels of miR-145, and its introduction decreased self-renewal through reductions in AKT signaling and stem cell marker (SOX2, OCT4, and NANOG) expression; JAM-A overexpression rescued these effects. These findings were predictive of patient survival, with a JAM-A/miR-145 signature robustly predicting poor patient prognosis.
  
  
  
  Our results link CSC-specific niche signaling to a microRNA regulatory network that is altered in glioblastoma and can be targeted to attenuate CSC self-renewal.
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Alvaro G
authors.initials: AG
authors.lastname: Alvarado
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Soumya M
authors.initials: SM
authors.lastname: Turaga
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Pratheesh
authors.initials: P
authors.lastname: Sathyan
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Erin E
authors.initials: EE
authors.lastname: Mulkearns-Hubert
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Balint
authors.initials: B
authors.lastname: Otvos
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Silver
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: James S
authors.initials: JS
authors.lastname: Hale
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: William A
authors.initials: WA
authors.lastname: Flavahan
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Pascal O
authors.initials: PO
authors.lastname: Zinn
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Maksim
authors.initials: M
authors.lastname: Sinyuk
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Meizhang
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Maheedhara R
authors.initials: MR
authors.lastname: Guda
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Kiran K
authors.initials: KK
authors.lastname: Velpula
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Tsung
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Ichiro
authors.initials: I
authors.lastname: Nakano
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Michael A
authors.initials: MA
authors.lastname: Vogelbaum
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Sadhan
authors.initials: S
authors.lastname: Majumder
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Jeremy N
authors.initials: JN
authors.lastname: Rich
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (A.G.A., S.M.T., E.E.M.-H., B.O., D.J.S., J.S.H., M.S., M.L., J.D.L.); Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio (A.G.A., J.N.R., J.D.L.); Department of Genetics, The University of Texas, MD Anderson Cancer Center, Houston, Texas (P.S., P.O.Z., S.M.); Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (W.A.F., J.N.R.); Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, China (M.L.); Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois (M.R.G., K.K.V., A.J.T.); Department of Neurological Surgery, The Ohio State University, Columbus, Ohio (I.N.); Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.); Case Comprehensive Cancer Center, Cleveland, Ohio (M.A.V., J.N.R., J.D.L.).
authors.forename: Justin D
authors.initials: JD
authors.lastname: Lathia
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology*; D064593:Heterografts; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D015815:Cell Adhesion Molecules; C407243:F11R protein, human; C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D011956:Receptors, Cell Surface
keywords: JAM-A; cancer stem cell; glioblastoma; miR-145