title: Effects of Mitochondrial Uncoupling Protein 2 Inhibition by Genipin in Human Cumulus Cells.
abstract: UCP2 plays a physiological role by regulating mitochondrial biogenesis, maintaining energy balance, ROS elimination, and regulating cellular autophagy in numerous tissues. But the exact roles of UCP2 in cumulus cells are still not clear. Genipin, a special UCP2 inhibitor, was added into the cultural medium to explore the roles of UCP2 in human cumulus cells. There were no significant differences in ATP and mitochondrial membrane potential levels in cumulus cells from UCP2 inhibiting groups as compared with the control. The levels of ROS and Mn-SOD were markedly elevated after UCP2 inhibited Genipin. However, the ratio of reduced GSH to GSSG significantly declined after treatment with Genipin. UCP2 inhibition by Genipin also resulted in obvious increase in the active caspase-3, which accompanied the decline of caspase-3 mRNA. The level of progesterone in culture medium declined obviously after Genipin treatment. But there was no significant difference in estradiol concentrations. This study indicated that UCP2 is expressed in human cumulus cells and plays important roles on mediate ROS production, apoptotic process, and steroidogenesis, suggesting UCP2 may be involved in regulation of follicle development and oocyte maturation and quality.
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang 325000, China.
authors.forename: Hongshan
authors.initials: H
authors.lastname: Ge
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang 325000, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang 325000, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Shan
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang 325000, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang 325000, China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Wang
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang 325000, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ling
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang 325000, China.
authors.forename: HaiTao
authors.initials: H
authors.lastname: Xi
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang 325000, China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Huang
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang 325000, China.
authors.forename: ChunFang
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang 325000, China.
authors.forename: Jeiqiang
authors.initials: J
authors.lastname: Lv
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001343:Autophagy / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D054885:Cumulus Cells / Q000187:drug effects*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007473:Ion Channels / Q000037:antagonists & inhibitors*; D039823:Iridoids / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D024101:Mitochondrial Proteins / Q000037:antagonists & inhibitors*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D000071246:Uncoupling Protein 2
chemical_list: D007473:Ion Channels; D039823:Iridoids; D024101:Mitochondrial Proteins; D017382:Reactive Oxygen Species; C000604389:UCP2 protein, human; D000071246:Uncoupling Protein 2; C007834:genipin; D013482:Superoxide Dismutase; D053148:Caspase 3; D005978:Glutathione