title: An integrated microfluidic system for bovine DNA purification and digital PCR detection.
abstract: In this paper, we described an integrated modularized microfluidic system that contained two distinct functional modules, one for nucleic acids (NA) extraction and the other for digital PCR (dPCR), allowing for detecting the bovine DNA in ovine tissue.
authors.affiliation: Research Center for Analytical Instrumentation, Institute of CyberSystems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, Zhejiang, PR China; College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, PR China.
authors.forename: Qingchang
authors.initials: Q
authors.lastname: Tian
authors.affiliation: Research Center for Analytical Instrumentation, Institute of CyberSystems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, Zhejiang, PR China. Electronic address: muying@zju.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Mu
authors.affiliation: Research Center for Analytical Instrumentation, Institute of CyberSystems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, Zhejiang, PR China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Research Center for Analytical Instrumentation, Institute of CyberSystems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, Zhejiang, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Song
authors.affiliation: Research Center for Analytical Instrumentation, Institute of CyberSystems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, Zhejiang, PR China.
authors.forename: Bingwen
authors.initials: B
authors.lastname: Yu
authors.affiliation: Research Center for Analytical Instrumentation, Institute of CyberSystems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, Zhejiang, PR China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Ma
authors.affiliation: Research Center for Analytical Instrumentation, Institute of CyberSystems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, Zhejiang, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.affiliation: Research Center for Analytical Instrumentation, Institute of CyberSystems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, Zhejiang, PR China.
authors.forename: Qinhan
authors.initials: Q
authors.lastname: Jin
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D008460:Meat / Q000032:analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D016133:Polymerase Chain Reaction / Q000295:instrumentation*
chemical_list: D004247:DNA
keywords: DNA purification; Digital PCR; Integrated microfluidic system; Polydimethylsiloxane