title: Changes in Laminin Expression Pattern during Early Differentiation of Human Embryonic Stem Cells.
abstract: Laminin isoforms laminin-511 and -521 are expressed by human embryonic stem cells (hESC) and can be used as a growth matrix to culture these cells under pluripotent conditions. However, the expression of these laminins during the induction of hESC differentiation has not been studied in detail. Furthermore, the data regarding the expression pattern of laminin chains in differentiating hESC is scarce. In the current study we aimed to fill this gap and investigated the potential changes in laminin expression during early hESC differentiation induced by retinoic acid (RA). We found that laminin-511 but not -521 accumulates in the committed cells during early steps of hESC differentiation. We also performed a comprehensive analysis of the laminin chain repertoire and found that pluripotent hESC express a more diverse range of laminin chains than shown previously. In particular, we provide the evidence that in addition to α1, α5, β1, β2 and γ1 chains, hESC express α2, α3, β3, γ2 and γ3 chain proteins and mRNA. Additionally, we found that a variant of laminin α3 chain-145 kDa-accumulated in RA-treated hESC showing that these cells produce prevalently specifically modified version of α3 chain in early phase of differentiation.
authors.affiliation: Department of Cell Biology, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Riia 23, 51010, Estonia.
authors.forename: Martin
authors.initials: M
authors.lastname: Pook
authors.affiliation: Department of Cell Biology, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Riia 23, 51010, Estonia.
authors.forename: Indrek
authors.initials: I
authors.lastname: Teino
authors.affiliation: Department of Cell Biology, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Riia 23, 51010, Estonia.
authors.forename: Ade
authors.initials: A
authors.lastname: Kallas
authors.affiliation: Department of Cell Biology, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Riia 23, 51010, Estonia.
authors.forename: Toivo
authors.initials: T
authors.lastname: Maimets
authors.affiliation: Department of Cell Biology, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Riia 23, 51010, Estonia.
authors.forename: Sulev
authors.initials: S
authors.lastname: Ingerpuu
authors.affiliation: Department of Cell Biology, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Riia 23, 51010, Estonia; Department of Biosciences and Nutrition, Karolinska Institutet, SE-141 83, Huddinge, Sweden.
authors.forename: Viljar
authors.initials: V
authors.lastname: Jaks
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D000066449:Human Embryonic Stem Cells / Q000378:metabolism* / Q000502:physiology*; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D051379:Mice; D020033:Protein Isoforms / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D007797:Laminin; D020033:Protein Isoforms; D012333:RNA, Messenger