title: Rapid and sensitive screening of some acidic micronutrients in infant foods by HPLC with fluorescent detector.
abstract: BACKGROUND
  Currently, commercially prepared complementary foods have become an important part of the diet of many infants and toddlers. But the method for simultaneous analysis of different types of micronutrient remains poorly investigated, which hinders the rapid and comprehensive quality control of infant foods. In the presented study, we first tried to employ the fluorescence labeling strategy combined with high-performance liquid chromatography-fluorescence detection for simultaneous determination of some acidic micronutrients including biotin, nicotinic acid, linolenic acid, eicosapentaenoic acid, docosahexaenoic acid, arachidonic acid and linoleic acid in infant foods.
  
  
  RESULTS
  2-(5-Benzoacridine) ethyl-p-toluenesulfonate was used as the fluorescence labeling reagent for simultaneous labeling of the seven components. The labeling conditions were optimized systematically by response surface methodology. The correlation coefficients for the calibration curves of the tested compounds ranged from 0.9991 to 0.9998. Limits of detection were in the range of 1.99-3.05 nmol L(-1) . Relative standard deviation values of retention time and peak area of seven compounds were less than 0.05% and 0.75%, respectively. The intra- and inter-day precision was in the range of 1.81-3.80% and 3.21-4.30%, respectively. When applied to analysis of several infant foods it showed good applicability.
  
  
  CONCLUSIONS
  The developed method has been proven to be simple, inexpensive, selective, sensitive, accurate and reliable for analysis of some acidic micronutrients in infant foodstuffs. Furthermore, this developed method also has powerful potential in the analysis of many other complementary foodstuffs. © 2015 Society of Chemical Industry.
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China.
authors.forename: Weiheng
authors.initials: W
authors.lastname: Kong
authors.affiliation: College of Chemistry and Environment Engineering, Beijing Technology and Business University, 11 Fucheng Road, Beijing, 100037, People's Republic of China.
authors.forename: Guangsen
authors.initials: G
authors.lastname: Fan
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Hu
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China.
authors.forename: Xianen
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China.|Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People's Republic of China.
authors.forename: Jinmao
authors.initials: J
authors.lastname: You
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes; D005504:Food Analysis / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D018977:Micronutrients / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D018977:Micronutrients
keywords: HPLC-FLD; acidic micronutrients; fluorescence labeling; infant foodstuffs