title: Characterization of annexin A2 in chicken follicle development: Evidence for its involvement in angiogenesis.
abstract: Annexin A2 (ANXA2) is a calcium-dependent, phospholipid-binding protein found in various cells and tissues. ANXA2 plays multiple roles in regulating cellular functions and is often over-expressed in different types of tumors including ovarian cancer. Others and we previously found that ANXA2 was up-regulated in the ovaries of hens with higher laying rate, indicated that ANXA2 is involved in avian follicle development. In this study, we found that ANXA2 mRNA expression increased during chicken ovary maturation and follicle development. In the pre-ovulatory follicles, ANXA2 expression level was significantly higher in theca cells than granulosa cells. In theca cells, ANXA2 expression could be stimulated by follicle-stimulating hormone (FSH) and estrogen but not luteinizing hormone (LH) or progesterone. The core promoter regions control the basal and FSH-induced ANXA2 gene expression were identified. Forced expression of ANXA2 could induce the expression of angiogenic factors and receptors in theca cells. Furthermore, ANXA2 overexpression resulted increased vascular endothelial growth factor A (VEGFA) secretion and theca cell proliferation. Current study not only provides the first evidence of expression and regulation of ANXA2 in chicken ovary, but also suggests that ANXA2 is involved in follicular angiogenesis and contributes to successful follicle development and ovulation.
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China; Department of Biology Science and Technology, Taishan University, Taian 271021, China.
authors.forename: Guiyu
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China; College of Life Science, Qufu Normal University, Qufu 273165, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Gynaecology, Taian Maternal and Child Health Care Hospital, Taian 271021, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Li
authors.initials: L
authors.lastname: Kang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Ma
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China. Electronic address: yljiang723@aliyun.com.
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000818:Animals; D017306:Annexin A2 / Q000502:physiology*; D002645:Chickens / Q000502:physiology; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000494:pharmacology; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D006080:Ovarian Follicle / Q000098:blood supply / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D011374:Progesterone / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013799:Theca Cells / Q000187:drug effects / Q000502:physiology
chemical_list: D017306:Annexin A2; D004967:Estrogens; D011374:Progesterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
keywords: ANXA2; Chicken; Follicle; Ovary; VEGF