title: Correlations of Gut Microbial Community Shift with Hepatic Damage and Growth Inhibition of Carassius auratus Induced by Pentachlorophenol Exposure.
abstract: Goldfish (Carassius auratus) were exposed to 0-100 μg/L pentachlorophenol (PCP) for 28 days to investigate the correlations of fish gut microbial community shift with the induced toxicological effects. PCP exposure caused accumulation of PCP in the fish intestinal tract in a time- and dose-dependent manner, while hepatic PCP reached the maximal level after a 21 day exposure. Under the relatively higher PCP stress, the fish body weight and liver weight were reduced and hepatic CAT and SOD activities were inhibited, demonstrating negative correlations with the PCP levels in liver and gut content (R < -0.5 and P < 0.05 each). Pyrosequencing of the 16S rRNA gene indicated that PCP exposure increased the abundance of Bacteroidetes in the fish gut. Within the Bacteroidetes phylum, the Bacteroides genus had the highest abundance, which was significantly correlated with PCP exposure dosage and duration (R > 0.5 and P < 0.05 each). Bioinformatic analysis revealed that Bacteroides showed quantitatively negative correlations with Chryseobacterium, Microbacterium, Arthrobacter, and Legionella in the fish gut, and the Bacteroidetes abundance, Bacteroides abundance, and Firmicutes/Bacteroidetes ratio played crucial roles in the reduction of body weight and liver weight under PCP stress. The results may extend our knowledge regarding the roles of gut microbiota in ecotoxicology.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Kan
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, China.
authors.forename: Fuzheng
authors.initials: F
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, China.
authors.forename: Xu-Xiang
authors.initials: XX
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, China.
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, China.
authors.forename: Shixiang
authors.initials: S
authors.lastname: Gao
mesh_terms: D000818:Animals; D041963:Bacteroidetes / Q000187:drug effects / Q000235:genetics; D002374:Catalase / Q000378:metabolism; D004781:Environmental Exposure / Q000032:analysis*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000378:metabolism; D006054:Goldfish / Q000254:growth & development* / Q000382:microbiology*; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D010416:Pentachlorophenol / Q000633:toxicity*; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D012336:RNA, Ribosomal, 16S; D010416:Pentachlorophenol; D002374:Catalase; D013482:Superoxide Dismutase