title: Enhanced biohydrogen production from sugarcane bagasse by Clostridium thermocellum supplemented with CaCO3.
abstract: Clostridium thermocellum ATCC 27405 was used to degrade sugarcane bagasse (SCB) directly for hydrogen production, which was significantly enhanced by supplementing medium with CaCO3. The effect of CaCO3 concentration on the hydrogen production was investigated. The hydrogen production was significantly enhanced with the CaCO3 concentration increased from 10mM to 20mM. However, with the CaCO3 concentration further increased from 20mM to 100mM, the hydrogen production didn't increase further. Under the optimal CaCO3 concentration of 20mM, the hydrogen production reached 97.83±5.19mmol/L from 2% sodium hydroxide-pretreated SCB, a 116.72% increase over the control (45.14±1.03mmol/L), and the yield of hydrogen production reached 4.89mmol H2/g SCBadded. Additionally, CaCO3 promoted the biodegradation of SCB and the growth of C. thermocellum. The stimulatory effects of CaCO3 on biohydrogen production are mainly attributed to the buffering capacity of carbonate. The study provides a novel strategy to enhance biohydrogen production from lignocellulose.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu, Guangzhou 510006, People's Republic of China.
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Tian
authors.affiliation: Guangzhou Sugarcane Industry Research Institute, Guangzhou 510316, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liang
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu, Guangzhou 510006, People's Republic of China; Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou 510006, People's Republic of China. Electronic address: mjzhu@scut.edu.cn.
authors.forename: Ming-Jun
authors.initials: MJ
authors.lastname: Zhu
mesh_terms: D001673:Biodegradation, Environmental; D056804:Biofuels; D002119:Calcium Carbonate / Q000008:administration & dosage / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D048013:Acetivibrio thermocellus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D031786:Saccharum / Q000187:drug effects / Q000378:metabolism*; D012972:Sodium Hydroxide / Q000494:pharmacology
chemical_list: D056804:Biofuels; D012972:Sodium Hydroxide; D006859:Hydrogen; D002482:Cellulose; C027433:bagasse; D002119:Calcium Carbonate
keywords: Biohydrogen; CaCO(3); Clostridium thermocellum; Sugarcane bagasse