title: The Functional Study of the N-Terminal Region of Influenza B Virus Nucleoprotein.
abstract: Influenza nucleoprotein (NP) is a major component of the ribonucleoprotein (vRNP) in influenza virus, which functions for the transcription and replication of viral genome. Compared to the nucleoprotein of influenza A (ANP), the N-terminal region of influenza B nucleoprotein (BNP) is much extended. By virus reconstitution, we found that the first 38 residues are essential for viral growth. We further illustrated the function of BNP by mini-genome reconstitution, fluorescence microscopy, electron microscopy, light scattering and gel shift. Results show that the N terminus is involved in the formation of both higher homo-oligomers of BNP and BNP-RNA complex.
authors.affiliation: Centre for Protein Science and Crystallography, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Centre for Protein Science and Crystallography, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
authors.forename: Mandy Ka-Han
authors.initials: MK
authors.lastname: Lam
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Qinfen
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Section of Virology, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Ruth
authors.initials: R
authors.lastname: Elderfield
authors.affiliation: Section of Virology, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Wendy S
authors.initials: WS
authors.lastname: Barclay
authors.affiliation: Centre for Protein Science and Crystallography, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
authors.forename: Pang-Chui
authors.initials: PC
authors.lastname: Shaw
mesh_terms: D009980:Influenza A virus / Q000737:chemistry / Q000378:metabolism; D009981:Influenza B virus / Q000737:chemistry* / Q000378:metabolism; D019590:Nucleocapsid Proteins; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000737:chemistry* / Q000378:metabolism; D014758:Viral Core Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D014758:Viral Core Proteins; C588186:nucleoprotein, influenza B virus