title: Human papillomavirus 16 E6 modulates the expression of host microRNAs in cervical cancer.
abstract: OBJECTIVE
  Human papillomavirus (HPV) infection is a prerequisite of developing cervical cancer, approximately half of which are associated with HPV type 16. There are reports that HPV can disturb the expression pattern of host miRNAs, but its mechanism is not well understood.
  
  
  METHODS
  In this study, we scanned 11 tumorigenesis related miRNAs in Hela cells that were overexpressed with HPV type 16 E6 protein.
  
  
  RESULTS
  We found the expression of miR-21 was upregulated by HPV type 16 E6 protein and meanwhile, the expression of miR-27a and miR-218 was downregulated. Furthermore, we identified that miR-21 overexpression could promote Hela and U2OS cells proliferation by targeting phosphatase-tensin homolog (PTEN), the result of which can be rescued by miR-21 inhibitor. In addition, E6 overexpression could also promote Hela cell migration and invasion.
  
  
  CONCLUSIONS
  Our results indicate that HPV infection and subsequent transformation take place through complex regulatory patterns of gene expression in the host cells, part of which are regulated by the E6 protein.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ben
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China.
authors.forename: Jingxia
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China.
authors.forename: Mingli
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China. Electronic address: doctorzheng52@yahoo.com.cn.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zheng
mesh_terms: D063646:Carcinogenesis / Q000235:genetics / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells / Q000473:pathology / Q000821:virology; D052162:Human papillomavirus 16 / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology / Q000821:virology*
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D035683:MicroRNAs; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins
keywords: E6 protein; cervical cancer; human papillomavirus; microRNA