title: Fast stimulated emission nanoscopy based on single molecule localization.
abstract: For super-resolution microscopy methods based on single molecule stochastic switching and localization, to simultaneously improve the spatial-temporal resolution, it is necessary to maximize the number of photons that can be collected from single molecules per unit time. Here, we describe a novel approach to enhance the signal intensity (collected photons per second) from fluorescence probes by introducing a stimulated emission (SE) optical process. This process is based on the following two properties: first, with reasonable parameters, the photon emission rate can be significantly increased with SE; and second, the SE photons, which are spatially coherent with the stimulation beam, are more favorable for collection than fluorescence. Theoretical results have shown that signal intensity from a single fluorescent molecule can be greatly improved with SE. We therefore showed, using SE in combination with single molecule localization methodology, that fast imaging at a rate of 0.05 s per reconstructed image with lateral resolutions of ∼30  nm can be obtained.
authors.forename: Xuehua
authors.initials: X
authors.lastname: Wang
authors.forename: Danni
authors.initials: D
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.forename: Hanben
authors.initials: H
authors.lastname: Niu
mesh_terms: D003198:Computer Simulation; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008027:Light; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D016011:Normal Distribution; D017785:Photons; D012815:Signal Processing, Computer-Assisted; D013269:Stochastic Processes
chemical_list: D005456:Fluorescent Dyes