title: Ultraviolet germicidal irradiation susceptibility of methicillin-resistant Staphylococcus aureus compared with methicillin-susceptible S. aureus.
abstract: Antibiotic misuse and overuse in both the healthcare and agricultural fields have dramatically increased the prevalence of antibiotic resistance in human pathogens. Two strains of methicillin-resistant Staphylococcus aureus (MRSA) (ATCC 43330 and a wild-type) and 1 strain of methicillin-susceptible S. aureus (ATCC 25923) were challenged (9 runs in triplicate) in a preliminary study with ultraviolet germicidal irradiation (UVGI) doses ranging from 0.25 to 3.00 mJ/cm(2). The mean percent kill was calculated for each strain when compared with the control plates (no exposure to UVGI). Then, each strain was challenged (22 runs in triplicate) with UVGI doses of 2.00, 2.50, and 3.00 mJ/cm(2). The results suggest a difference between the doses required to disinfect surfaces with each strain. Assuming a standard error rate of α = 0.05, there was a significant difference in variance between the MRSA (ATCC 43330 and wild type) strains and the S. aureus (ATCC 25923) methicillin-susceptible strain.
authors.affiliation: a Science and Health Department, University of Cincinnati Clermont College, Batavia, OH 45103, USA.
authors.forename: Christopher F
authors.initials: CF
authors.lastname: Green
authors.affiliation: a Science and Health Department, University of Cincinnati Clermont College, Batavia, OH 45103, USA.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Elbe
authors.affiliation: a Science and Health Department, University of Cincinnati Clermont College, Batavia, OH 45103, USA.
authors.forename: Tyler D
authors.initials: TD
authors.lastname: Neal
authors.affiliation: b Department of Environmental, Agricultural and Occupational Health, University of Nebraska Medical Center College of Public Health, Omaha, NE 68198-4355, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Lowe
authors.affiliation: b Department of Environmental, Agricultural and Occupational Health, University of Nebraska Medical Center College of Public Health, Omaha, NE 68198-4355, USA.|c Department of Environmental Health, Indiana University School of Public Health-Bloomington, 1025 E 7th Street, Room No. 111C, Bloomington, IN 47405, USA.
authors.forename: Shawn G
authors.initials: SG
authors.lastname: Gibbs
mesh_terms: D004203:Disinfection; D016106:Methicillin Resistance; D055624:Methicillin-Resistant Staphylococcus aureus / Q000528:radiation effects*; D008826:Microbial Sensitivity Tests; D015203:Reproducibility of Results; D013211:Staphylococcus aureus / Q000528:radiation effects*; D014466:Ultraviolet Rays
keywords: Staphylococcus aureus; Staphylococcus aureus résistant à la méthicilline; irradiation germicide aux ultraviolets; methicillin-resistant Staphylococcus aureus; ultraviolet germicidal irradiation