title: The Inhibition of Escherichia coli Biofilm Formation by Gallium Nitrate-Modified Titanium.
abstract: Periprosthetic infections are notoriously difficult to treat due to biofilm formation. Previously, we reported that gallium-EDTA attached to PVC (polyvinyl chloride) surface could prevent bacterial colonization. Herein we examined the effect of this gallium-EDTA complex on Escherichia coli biofilm formation on titanium. It was clearly demonstrated that gallium nitrate significantly inhibited the growth and auto-aggregation of Escherichia coli. Furthermore, titanium with gallium-EDTA coating resisted bacterial colonization as indicated by crystal violet staining. When the chips were immersed in human serum and incubated at 37 °C, they demonstrated significant antimicrobial activity after more than 28 days of incubation. These findings indicate that gallium-EDTA coating of implants can result in a surface that can resist bacterial colonization. This technology holds great promise for the prevention and treatment of periprosthetic infections.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Qiu
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Chen
authors.forename: Lianming
authors.initials: L
authors.lastname: Liao
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D049109:Cell Proliferation / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D004058:Diffusion; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development*; D005708:Gallium / Q000737:chemistry / Q000494:pharmacology*; D008422:Materials Testing; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D019736:Prostheses and Implants / Q000382:microbiology*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D005708:Gallium; D014025:Titanium; C027235:gallium nitrate