title: Supramolecular Translation of Enzymatically Triggered Disassembly of Micelles into Tunable Fluorescent Responses.
abstract: The need for advanced fluorescent imaging and delivery platforms has motivated the development of smart probes that change their fluorescence in response to external stimuli. Here a new molecular design of fluorescently labeled PEG-dendron hybrids that self-assemble into enzyme-responsive micelles with tunable fluorescent responses is reported. In the assembled state, the fluorescence of the dyes is quenched or shifted due to intermolecular interactions. Upon enzymatic cleavage of the hydrophobic end-groups, the labeled polymeric hybrids become hydrophilic, and the micelles disassemble. This supramolecular change is translated into a spectral response as the dye-dye interactions are eliminated and the intrinsic fluorescence is regained. We demonstrate the utilization of this molecular design to generate both Turn-On and spectral shift responses by adjusting the type of the labeling dye. This approach enables transformation of non-responsive labeling dyes into smart fluorescent probes.
authors.affiliation: Department of Organic Chemistry, School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978 (Israel).|Tel Aviv University Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv, 69978 (Israel).
authors.forename: Marina
authors.initials: M
authors.lastname: Buzhor
authors.affiliation: Department of Organic Chemistry, School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978 (Israel).|Tel Aviv University Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv, 69978 (Israel).
authors.forename: Assaf J
authors.initials: AJ
authors.lastname: Harnoy
authors.affiliation: Department of Physical Chemistry, School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978 (Israel).|Tel Aviv University Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv, 69978 (Israel).
authors.forename: Einat
authors.initials: E
authors.lastname: Tirosh
authors.affiliation: Department of Physical Chemistry, School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978 (Israel).|Tel Aviv University Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv, 69978 (Israel).
authors.forename: Ayana
authors.initials: A
authors.lastname: Barak
authors.affiliation: Department of Physical Chemistry, School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978 (Israel).|Tel Aviv University Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv, 69978 (Israel).
authors.forename: Tal
authors.initials: T
authors.lastname: Schwartz
authors.affiliation: Department of Organic Chemistry, School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978 (Israel). amirroey@tau.ac.il.|Tel Aviv University Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv, 69978 (Israel). amirroey@tau.ac.il.
authors.forename: Roey J
authors.initials: RJ
authors.lastname: Amir
mesh_terms: D050091:Dendrimers / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004950:Esterases / Q000737:chemistry* / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry
chemical_list: D050091:Dendrimers; D004337:Drug Carriers; D005456:Fluorescent Dyes; D008823:Micelles; D011108:Polymers; D011092:Polyethylene Glycols; D004950:Esterases
keywords: enzymes; fluorescence; micelles; nanocarriers; stimuli-responsive polymers