title: [Interleukin-37 inhibits the motility and migration of mouse splenic dendritic cells].
abstract: OBJECTIVE
  To investigate the effect of interleukin-37 (IL-37) on the motility and migration of mouse splenic dendritic cells (DCs).
  
  
  METHODS
  Magnetic bead cell sorting was used to isolate mouse splenic DCs, and flow cytometry was used to analyze the purity. DCs were visualized in real time by PerkinElmer laser confocal microscopy and analyzed by Volocity image analysis software. The alteration of chemokine (C motif) receptor 1 (XCR1) mRNA was revealed by microarray analysis and confirmed by real-time PCR and flow cytometry. Migration ability of DCs was detected by Transwell(TM) migration assay.
  
  
  RESULTS
  The purity of isolated DCs was over 95%. The treatment of IL-37 reduced the velocity of DCs mobility, decreased XCR1 expression and depressed the cell migration ability.
  
  
  CONCLUSIONS
  IL-37 can inhibit the motility and migration of mouse splenic DCs.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, China.
authors.forename: Yuechen
authors.initials: Y
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, China.
authors.forename: Wantong
authors.initials: W
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, China.
authors.forename: Zhina
authors.initials: Z
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Shen
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D007375:Interleukin-1 / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D019707:Receptors, Chemokine / Q000032:analysis; D013154:Spleen / Q000166:cytology
chemical_list: C508587:IL37 protein, human; D007375:Interleukin-1; D019707:Receptors, Chemokine; C551408:XC chemokine receptor 1, mouse