title: A study on metabolic prowess of Pseudomonas sp. RPT 52 to degrade imidacloprid, endosulfan and coragen.
abstract: A bacterial strain identified as Pseudomonas sp. RPT 52, was isolated from an agricultural field by soil enrichment technique. The bacterial strain was able to metabolize three different chlorinated pesticides; imidacloprid, endosulfan and coragen (belonging to neonicotinoid, organochlorine and anthranillic diamide categories, respectively). RPT 52 was able to degrade 46.5%, 96.6%, 92.7% and 80.16% of 0.5 mM of imidacloprid, endosulfan α, endosulfan β and coragen, respectively, in minimal medium over a period of 40 h, when provided as sole source of carbon and energy. Degradation kinetics showed that imidacloprid, endosulfan α and endosulfan β followed first order kinetics whereas coragen followed zero order kinetics. Toxicity studies show reduction in toxicity of the parent compound when degraded by RPT 52. Laboratory scale, soil microcosm studies showed that strain RPT 52 is a suitable candidate for bioremediation of endosulfan and coragen contaminated sites. Thus, RPT 52 holds potential for toxicity reduction in the affected environment.
authors.affiliation: Centre of Excellence in Disaster Mitigation and Management, IIT Roorkee, Roorkee, Uttarakhand 247 667, India; Uttarakhand Technical University, Dehradun, Uttarakhand 248 007, India. Electronic address: manasi149@gmail.com.
authors.forename: Manasi
authors.initials: M
authors.lastname: Gupta
authors.affiliation: Department of Biotechnology, Indian Institute of Technology (IIT) Roorkee, Roorkee, Uttarakhand 247 667, India. Electronic address: samarth8392@gmail.com.
authors.forename: Samarth
authors.initials: S
authors.lastname: Mathur
authors.affiliation: Department of Biotechnology, Indian Institute of Technology (IIT) Roorkee, Roorkee, Uttarakhand 247 667, India. Electronic address: tarundbt@gmail.com.
authors.forename: Tarun K
authors.initials: TK
authors.lastname: Sharma
authors.affiliation: Department of Biotechnology, Indian Institute of Technology (IIT) Roorkee, Roorkee, Uttarakhand 247 667, India. Electronic address: manish.rana@nic.in.
authors.forename: Manish
authors.initials: M
authors.lastname: Rana
authors.affiliation: Centre of Excellence in Disaster Mitigation and Management, IIT Roorkee, Roorkee, Uttarakhand 247 667, India. Electronic address: garryfce@gmail.com.
authors.forename: Ajay
authors.initials: A
authors.lastname: Gairola
authors.affiliation: Department of Biotechnology, Indian Institute of Technology (IIT) Roorkee, Roorkee, Uttarakhand 247 667, India; Centre of Excellence in Disaster Mitigation and Management, IIT Roorkee, Roorkee, Uttarakhand 247 667, India. Electronic address: navnifbs@iitr.ac.in.
authors.forename: Naveen K
authors.initials: NK
authors.lastname: Navani
authors.affiliation: Department of Biotechnology, Indian Institute of Technology (IIT) Roorkee, Roorkee, Uttarakhand 247 667, India; Centre of Excellence in Disaster Mitigation and Management, IIT Roorkee, Roorkee, Uttarakhand 247 667, India. Electronic address: rpathfbs@iitr.ac.in.
authors.forename: Ranjana
authors.initials: R
authors.lastname: Pathania
mesh_terms: D001673:Biodegradation, Environmental; D002470:Cell Survival / Q000187:drug effects; D004726:Endosulfan / Q000378:metabolism* / Q000633:toxicity; D006801:Humans; D007093:Imidazoles / Q000378:metabolism* / Q000633:toxicity; D007306:Insecticides / Q000378:metabolism* / Q000633:toxicity; D061986:MCF-7 Cells; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000378:metabolism* / Q000633:toxicity; D011549:Pseudomonas / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism* / Q000633:toxicity; D062367:ortho-Aminobenzoates / Q000378:metabolism* / Q000633:toxicity
chemical_list: D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D012989:Soil Pollutants; D062367:ortho-Aminobenzoates; C082359:imidacloprid; C517733:chlorantranilipole; D004726:Endosulfan
keywords: Biodegradation; Coragen; Endosulfan; Imidacloprid; Pesticide degradation