title: Up-regulation of HDAC9 promotes cell proliferation through suppressing p53 transcription in osteosarcoma.
abstract: Increasing studies have demonstrated that altered expression of histone deacetylases (HDACs) plays a critical role in the tumorigenesis through up-regulation or down-regulation of key genes involved in cell proliferation, cell-cycle regulation and apoptosis. In the present study, the expression and function of HDAC9 were investigated in osteosarcoma. Quantitative real-time PCR and Western blot analysis found that HDAC9 was up-regulated in osteosarcoma tissues, when compared with that in adjacent normal tissues. In vitro studies further demonstrated that overexpression of HDAC9 in U2OS and MG63 cells promoted cell proliferation and invasion. Using chromatin immunoprecipitation (ChIP) assay, we found that HDAC9 epigenetically repressed p53 transcription through binding to its proximal promoter region. Therefore, our data suggest an important role for HDAC9/p53 regulatory pathway in the osteosarcoma progression.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University Zhengzhou 450000, Henan, China ; Department of Bone and Soft Tissue Tumors, Zhengzhou University Cancer Hospital Zhengzhou 450008, Henan, China.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University Zhengzhou 450000, Henan, China.
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: Wang
authors.affiliation: Department of Bone and Soft Tissue Tumors, Zhengzhou University Cancer Hospital Zhengzhou 450008, Henan, China.
authors.forename: Qi-Qing
authors.initials: QQ
authors.lastname: Cai
authors.affiliation: Department of Bone and Soft Tissue Tumors, Zhengzhou University Cancer Hospital Zhengzhou 450008, Henan, China.
authors.forename: Jia-Qiang
authors.initials: JQ
authors.lastname: Wang
authors.affiliation: Department of Bone and Soft Tissue Tumors, Zhengzhou University Cancer Hospital Zhengzhou 450008, Henan, China.
authors.forename: Wei-Tao
authors.initials: WT
authors.lastname: Yao
keywords: HDAC9; Osteosarcoma; cell proliferation; epigenetics; p53