title: A 3D µPAD based on a multi-enzyme organic-inorganic hybrid nanoflower reactor.
abstract: This work reports on the development of a 3D microfluidic paper-based device (3D µPAD) for glucose detection using organic-inorganic hybrid nanoflower technology to immobilize the bi-enzymatic system (glucose oxidase and horseradish peroxidase). The system is based on nanoflowerssupported on cellulose paper (the microreactor zone) coupled to 3,3',5,5'-tetramethylbenzidine (TMB) as the colorimetric probe in the detection zone. We used a digital camera for the quantitative analysis of glucose with the S coordinate of the HSV color space as the analytical parameter. Under optimal operational conditions, linearity was observed for glucose concentrations up to 300 μM, with a detection limit of 15.6 µM. The biosensor is reusable and remains stable for 75 days in conventional storage conditions.
authors.affiliation: ECsens, Department of Analytical Chemistry, Faculty of Sciences, University of Granada, E-18071 Granada, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Ariza-Avidad
authors.affiliation: ECsens, Department of Analytical Chemistry, Faculty of Sciences, University of Granada, E-18071 Granada, Spain. Electronic address: alfonsos@ugr.es.
authors.forename: A
authors.initials: A
authors.lastname: Salinas-Castillo
authors.affiliation: ECsens, Department of Analytical Chemistry, Faculty of Sciences, University of Granada, E-18071 Granada, Spain.
authors.forename: L F
authors.initials: LF
authors.lastname: Capitán-Vallvey
mesh_terms: D003124:Colorimetry / Q000295:instrumentation*; D004209:Disposable Equipment; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017743:Equipment Reuse; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D009097:Multienzyme Complexes / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010209:Paper; D010316:Particle Size; D066330:Printing, Three-Dimensional
chemical_list: D004800:Enzymes, Immobilized; D009097:Multienzyme Complexes; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D005947:Glucose
keywords: Colorimetric detection; Enzymatic microreactor; GOx-HRP nanoflowers; Glucose monitoring.; µPADs