title: In Vitro and In Vivo Response to Low-Modulus PMMA-Based Bone Cement.
abstract: The high stiffness of acrylic bone cements has been hypothesized to contribute to the increased number of fractures encountered after vertebroplasty, which has led to the development of low-modulus cements. However, there is no data available on the in vivo biocompatibility of any low-modulus cement. In this study, the in vitro cytotoxicity and in vivo biocompatibility of two types of low-modulus acrylic cements, one modified with castor oil and one with linoleic acid, were evaluated using human osteoblast-like cells and a rodent model, respectively. While the in vitro cytotoxicity appeared somewhat affected by the castor oil and linoleic acid additions, no difference could be found in the in vivo response to these cements in comparison to the base, commercially available cement, in terms of histology and flow cytometry analysis of the presence of immune cells. Furthermore, the in vivo radiopacity of the cements appeared unaltered. While these results are promising, the mechanical behavior of these cements in vivo remains to be investigated.
authors.affiliation: Division of Orthopedics, Department of Surgical Sciences, Uppsala University Hospital, Entrance 61, 751 85 Uppsala, Sweden ; Division of Applied Materials Science, Department of Engineering Sciences, Ångström Laboratory, Uppsala University, P.O. Box 534, 751 21 Uppsala, Sweden.
authors.forename: Elin
authors.initials: E
authors.lastname: Carlsson
authors.affiliation: Division of Applied Materials Science, Department of Engineering Sciences, Ångström Laboratory, Uppsala University, P.O. Box 534, 751 21 Uppsala, Sweden.
authors.forename: Gemma
authors.initials: G
authors.lastname: Mestres
authors.affiliation: Division of Orthopedics, Department of Surgical Sciences, Uppsala University Hospital, Entrance 61, 751 85 Uppsala, Sweden.
authors.forename: Kiatnida
authors.initials: K
authors.lastname: Treerattrakoon
authors.affiliation: Division of Applied Materials Science, Department of Engineering Sciences, Ångström Laboratory, Uppsala University, P.O. Box 534, 751 21 Uppsala, Sweden.
authors.forename: Alejandro
authors.initials: A
authors.lastname: López
authors.affiliation: Division of Applied Materials Science, Department of Engineering Sciences, Ångström Laboratory, Uppsala University, P.O. Box 534, 751 21 Uppsala, Sweden.
authors.forename: Marjam
authors.initials: M
authors.lastname: Karlsson Ott
authors.affiliation: Division of Orthopedics, Department of Surgical Sciences, Uppsala University Hospital, Entrance 61, 751 85 Uppsala, Sweden.
authors.forename: Sune
authors.initials: S
authors.lastname: Larsson
authors.affiliation: Division of Applied Materials Science, Department of Engineering Sciences, Ångström Laboratory, Uppsala University, P.O. Box 534, 751 21 Uppsala, Sweden.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Persson
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D001843:Bone Cements / Q000494:pharmacology*; D002460:Cell Line; D019245:Compressive Strength; D006801:Humans; D008297:Male; D008422:Materials Testing; D010006:Osteoblasts / Q000187:drug effects*; D019904:Polymethyl Methacrylate / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D054854:Vertebroplasty / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D001843:Bone Cements; D019904:Polymethyl Methacrylate