title: Bacterial and Archaeal Communities Variability Associated with Upwelling and Anthropogenic Pressures in the Protection Area of Arraial do Cabo (Cabo Frio region - RJ).
abstract: Upwelling systems contain a high diversity of pelagic microorganisms and their composition and activity are defined by factors like temperature and nutrient concentration. Denaturing gradient gel electrophoresis (DGGE) technique was used to verify the spatial and temporal genetic variability of Bacteria and Archaea in two stations of the Arraial do Cabo coastal region, one under upwelling pressure and another under anthropogenic pressure. In addition, biotic and abiotic variables were measured in surface and deep waters from three other stations between these stations. Six samplings were done during a year and adequately represented the degrees of upwelling and anthropogenic pressures to the system. Principal Component Analysis (PCA) showed negative correlations between the concentrations of ammonia and phosphorous with prokaryotic secondary production and the total heterotrophic bacteria. PCA also showed negative correlation between temperature and the abundance of prokaryotic cells. Bacterial and archaeal compositions were changeable as were the oceanographic conditions, and upwelling had a regional pressure while anthropogenic pressure was punctual. We suggest that the measurement of prokaryotic secondary production was associated with both Bacteria and Archaea activities, and that substrate availability and temperature determine nutrients cycling.
authors.affiliation: Instituto de Ciências do Marz Almirante Paulo Moreira, Arraial do Cabo, RJ, BR.
authors.forename: Sergio A
authors.initials: SA
authors.lastname: Coelho-Souza
authors.affiliation: Departamento de Ciências, Faculdade de Formação de Professores, Universidade do Estado do Rio de Janeiro, São Gonçalo, RJ, BR.
authors.forename: Fábio V
authors.initials: FV
authors.lastname: Araújo
authors.affiliation: Departamento de Ciências Exatas e Biológica, Universidade Federal de São João del-Rei, Sete Lagoas, MG, BR.
authors.forename: Juliano C
authors.initials: JC
authors.lastname: Cury
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BR.
authors.forename: Hugo E
authors.initials: HE
authors.lastname: Jesus
authors.affiliation: Centro de Tecnologia, UFRJ, Rio de Janeiro, RJ, BR.
authors.forename: Gilberto C
authors.initials: GC
authors.lastname: Pereira
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BR.
authors.forename: Jean R D
authors.initials: JR
authors.lastname: Guimarães
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BR.
authors.forename: Raquel S
authors.initials: RS
authors.lastname: Peixoto
authors.affiliation: Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, BR.
authors.forename: Alberto M R
authors.initials: AM
authors.lastname: Dávila
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BR.
authors.forename: Alexandre S
authors.initials: AS
authors.lastname: Rosado
mesh_terms: D000641:Ammonia / Q000032:analysis; D001105:Archaea / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D001938:Brazil; D058645:Denaturing Gradient Gel Electrophoresis; D025341:Principal Component Analysis; D012623:Seawater / Q000382:microbiology; D013696:Temperature
chemical_list: D000641:Ammonia