title: Pri-miR-34b/c rs4938723 polymorphism contributes to acute lymphoblastic leukemia susceptibility in Chinese children.
abstract: A polymorphism rs4938723 (T > C) within the promoter region of pri-miR-34b/c has been found to not only affect the expression of mature miR-34b/c but also contribute to the susceptibility to several cancer types. We designed a case-control study to evaluate the role of rs4938723 in childhood acute lymphoblastic leukemia (ALL). The rs4938723 CC genotype was significantly associated with reduced ALL risk (p = 0.003, ORadjusted = 0.51, 95% CI = 0.33-0.80 for CC vs. TT). Stratification analyses showed the differences were pronounced in older (> 6 years), male subjects, as well as in patients in low risk and T-ALL subtypes. The in vitro luciferase assays in Jurkat and K-562 cell lines showed that the transcription activity of miR-34b/c was increased when T allele transited to C allele (p < 0.05). In conclusion, rs4938723 genetic variant contributed to the susceptibility to Chinese childhood ALL by influencing the transcription activity of miR-34b/c promoter.
authors.affiliation: a Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers , Prevention and Treatment, Cancer Center, Nanjing Medical University , Nanjing , China ;|b Department of Genetic Toxicology, the Key Laboratory of Modern Toxicology of Ministry of Education , School of Public Health, Nanjing Medical University , Nanjing , China ;
authors.forename: Na
authors.initials: N
authors.lastname: Tong
authors.affiliation: a Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers , Prevention and Treatment, Cancer Center, Nanjing Medical University , Nanjing , China ;|b Department of Genetic Toxicology, the Key Laboratory of Modern Toxicology of Ministry of Education , School of Public Health, Nanjing Medical University , Nanjing , China ;
authors.forename: Haiyan
authors.initials: H
authors.lastname: Chu
authors.affiliation: a Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers , Prevention and Treatment, Cancer Center, Nanjing Medical University , Nanjing , China ;|b Department of Genetic Toxicology, the Key Laboratory of Modern Toxicology of Ministry of Education , School of Public Health, Nanjing Medical University , Nanjing , China ;
authors.forename: Meilin
authors.initials: M
authors.lastname: Wang
authors.affiliation: c Department of Hematology and Oncology , The Affiliated Nanjing Children's Hospital of Nanjing Medical University , Nanjing , China ;
authors.forename: Yao
authors.initials: Y
authors.lastname: Xue
authors.affiliation: a Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers , Prevention and Treatment, Cancer Center, Nanjing Medical University , Nanjing , China ;|b Department of Genetic Toxicology, the Key Laboratory of Modern Toxicology of Ministry of Education , School of Public Health, Nanjing Medical University , Nanjing , China ;
authors.forename: Mulong
authors.initials: M
authors.lastname: Du
authors.affiliation: c Department of Hematology and Oncology , The Affiliated Nanjing Children's Hospital of Nanjing Medical University , Nanjing , China ;
authors.forename: Lingling
authors.initials: L
authors.lastname: Lu
authors.affiliation: c Department of Hematology and Oncology , The Affiliated Nanjing Children's Hospital of Nanjing Medical University , Nanjing , China ;
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: c Department of Hematology and Oncology , The Affiliated Nanjing Children's Hospital of Nanjing Medical University , Nanjing , China ;
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: c Department of Hematology and Oncology , The Affiliated Nanjing Children's Hospital of Nanjing Medical University , Nanjing , China ;
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Fang
authors.affiliation: d Department of Hematology and Oncology , Soochow Children's Hospital Affiliated to Soochow University , Suzhou , China ;
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: a Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers , Prevention and Treatment, Cancer Center, Nanjing Medical University , Nanjing , China ;|b Department of Genetic Toxicology, the Key Laboratory of Modern Toxicology of Ministry of Education , School of Public Health, Nanjing Medical University , Nanjing , China ;
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wu
authors.affiliation: a Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers , Prevention and Treatment, Cancer Center, Nanjing Medical University , Nanjing , China ;|b Department of Genetic Toxicology, the Key Laboratory of Modern Toxicology of Ministry of Education , School of Public Health, Nanjing Medical University , Nanjing , China ;
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: e Department of Children's Healthcare , The Second Affiliated Hospital of Nanjing Medical University , Nanjing , China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Sheng
mesh_terms: D000293:Adolescent; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002681:China; D004777:Environment; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000453:epidemiology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012306:Risk
chemical_list: C522425:MIRN34 microRNA, human; D035683:MicroRNAs
keywords: childhood ALL; genetic susceptibility; miR-34b/c; molecular epidemiology; transcription activity