title: Collaborative science in the next-generation sequencing era: a viewpoint on how to combine exome sequencing data across sites to identify novel disease susceptibility genes.
abstract: The purpose of this article is to inform readers about technical challenges that we encountered when assembling exome sequencing data from the 'Simplifying Complex Exomes' (SIMPLEXO) consortium-whose mandate is the discovery of novel genes predisposing to breast and ovarian cancers. Our motivation is to share these obstacles-and our solutions to them-as a means of communicating important technical details that should be discussed early in projects involving massively parallel sequencing.
authors.forename: Steven N
authors.initials: SN
authors.lastname: Hart
authors.forename: Kara N
authors.initials: KN
authors.lastname: Maxwell
authors.forename: Tinu
authors.initials: T
authors.lastname: Thomas
authors.forename: Vignesh
authors.initials: V
authors.lastname: Ravichandran
authors.forename: Bradley
authors.initials: B
authors.lastname: Wubberhorst
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Klein
authors.forename: Kasmintan
authors.initials: K
authors.lastname: Schrader
authors.forename: Csilla
authors.initials: C
authors.lastname: Szabo
authors.forename: Jeffrey N
authors.initials: JN
authors.lastname: Weitzel
authors.forename: Susan L
authors.initials: SL
authors.lastname: Neuhausen
authors.forename: Katherine
authors.initials: K
authors.lastname: Nathanson
authors.forename: Kenneth
authors.initials: K
authors.lastname: Offit
authors.forename: Fergus J
authors.initials: FJ
authors.lastname: Couch
authors.forename: Joseph
authors.initials: J
authors.lastname: Vijai
mesh_terms: D059472:Exome; D020022:Genetic Predisposition to Disease; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans
keywords: exome; genomics