title: Early detection of extended-spectrum β-lactamase from blood culture positive for an Enterobacteriaceae using βLACTA test.
abstract: Bacterial pellets from Enterobacteriaceae positive blood cultures prepared using ammonium chloride were tested for rapid detection of β-lactamase using the commercial βLACTA test and read after 30 minutes. During 7 months, 137 bacterial pellets were tested prospectively. βLACTA test exhibited a sensitivity of 75% and a specificity of 100% for the detection of third-generation cephalosporin resistance. False negative tests were mainly observed with hyperproduced chromosomal or plasmid-borne AmpC.
authors.affiliation: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
authors.forename: Guy
authors.initials: G
authors.lastname: Prod'hom
authors.affiliation: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
authors.forename: Christian
authors.initials: C
authors.lastname: Durussel
authors.affiliation: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland ; Service of Hospital Preventive Medicine, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
authors.forename: Dominique
authors.initials: D
authors.lastname: Blanc
authors.affiliation: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
authors.forename: Antony
authors.initials: A
authors.lastname: Croxatto
authors.affiliation: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
authors.forename: Gilbert
authors.initials: G
authors.lastname: Greub
keywords: Ammonium chloride; blood culture; cephalosporins; chromogenic cephalosporin; rapid testing; β-lactamase