title: Recombinant phosphoprotein based single serum dilution ELISA for rapid serological detection of Newcastle disease virus.
abstract: Newcastle disease virus (NDV) is the causative agent of a highly contagious disease in avian species. All strains of NDV belong to avian paramyxovirus serotype-1. The disease is endemic in different parts of the world and vaccination is the only way to protect birds from NDV infection. The virus non-structural phosphoprotein (P) is the second most abundant protein and a major modulator of viral replication. Although P protein shows lesser evolutionary divergence among NDV isolates, it is known to be highly divergent among different avian paramyxovirus serotypes. In the present study, a recombinant P protein based single serum dilution ELISA was developed which showed better sensitivity, specificity and accuracy as compared to conventional methods for NDV detection. The recombinant P protein based ELISA could be an alternative to existing diagnostics against NDV infection in chickens.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
authors.forename: Moushumee
authors.initials: M
authors.lastname: Das
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India. Electronic address: sachinku@iitg.ernet.in.
authors.forename: Sachin
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001717:Birds; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D009521:Newcastle Disease / Q000175:diagnosis*; D009522:Newcastle disease virus / Q000276:immunology* / Q000302:isolation & purification*; D010750:Phosphoproteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*; D013997:Time Factors; D014764:Viral Proteins / Q000276:immunology*; D014773:Virology / Q000379:methods*
chemical_list: D000914:Antibodies, Viral; C055691:P phosphoprotein, Newcastle disease virus; D010750:Phosphoproteins; D014764:Viral Proteins
keywords: Negative sense RNA; Newcastle disease virus; Pathogenicity; Phosphoprotein; Prokaryotic expression