title: Specific and Sensitive Detection of the Pear Scab Fungus Venturia nashicola by SYBR Green Real-Time PCR.
abstract: A new improved PCR method has been developed for the rapid, reliable, and sensitive detection of Venturia nashicola, a destructive pathogen of scab disease in Japanese pear. The translation elongation factor-1 alpha gene-derived PCR primers specifically amplified a 257-bp-sized DNA band of the target gene from the genomic DNA of V. nashicola. No amplicon was produced from the genomic DNA of other Venturia spp. and reference fungal species tested. With the high detection limit of 10 fg DNA content, our real-time method could be used for the quarantine inspection and field monitoring of V. nashicola.
authors.affiliation: Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Yeo Hong
authors.initials: YH
authors.lastname: Yun
authors.affiliation: Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Seong Kwon
authors.initials: SK
authors.lastname: Yoon
authors.affiliation: Department of Biology, Sunchon National University, Suncheon 540-950, Republic of Korea.
authors.forename: Jae Sung
authors.initials: JS
authors.lastname: Jung
authors.affiliation: Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 330-714, Republic of Korea.
authors.forename: Seong Hwan
authors.initials: SH
authors.lastname: Kim
mesh_terms: D001203:Ascomycota / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology*; D031989:Pyrus / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D005656:Fungal Proteins; D020648:Peptide Elongation Factor 1
keywords: Pear scab; Venturia nashicola; real-time PCR; translation elongation factor-1 alpha gene