title: Conditional deletion of nonmuscle myosin II-A in mouse tongue epithelium results in squamous cell carcinoma.
abstract: To investigate the contribution of nonmuscle myosin II-A (NM II-A) to early cardiac development we crossed Myh9 floxed mice and Nkx2.5 cre-recombinase mice. Nkx2.5 is expressed in the early heart (E7.5) and later in the tongue epithelium. Mice homozygous for deletion of NM II-A (A(Nkx)/A(Nkx)) are born at the expected ratio with normal hearts, but consistently develop an invasive squamous cell carcinoma (SCC) of the tongue (32/32 A(Nkx)/A(Nkx)) as early as E17.5. To assess reproducibility a second, independent line of Myh9 floxed mice derived from a different embryonic stem cell clone was tested. This second line also develops SCC indistinguishable from the first (15/15). In A(Nkx)/A(Nkx) mouse tongue epithelium, genetic deletion of NM II-A does not affect stabilization of TP53, unlike a previous report for SCC. We attribute the consistent, early formation of SCC with high penetrance to the role of NM II in maintaining mitotic stability during karyokinesis.
authors.affiliation: Laboratory of Molecular Cardiology, NHLBI, Bethesda, MD 20814, USA.
authors.forename: Mary Anne
authors.initials: MA
authors.lastname: Conti
authors.affiliation: Head and Neck Surgery Branch, NIDCD, Bethesda, MD 20892, USA.
authors.forename: Anthony D
authors.initials: AD
authors.lastname: Saleh
authors.affiliation: Division of Veterinary Resources, Office of Research Services, NIH, Bethesda, Maryland 20892-5520, USA.
authors.forename: Lauren R
authors.initials: LR
authors.lastname: Brinster
authors.affiliation: Head and Neck Surgery Branch, NIDCD, Bethesda, MD 20892, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Head and Neck Surgery Branch, NIDCD, Bethesda, MD 20892, USA.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Head and Neck Surgery Branch, NIDCD, Bethesda, MD 20892, USA.
authors.forename: Shaleeka
authors.initials: S
authors.lastname: Cornelius
authors.affiliation: Transgenic Core Facility, NHLBI, NIH, Bethesda, MD, 20892, USA.
authors.forename: Chengyu
authors.initials: C
authors.lastname: Liu
authors.affiliation: Laboratory of Molecular Cardiology, NHLBI, Bethesda, MD 20814, USA.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Ma
authors.affiliation: Head and Neck Surgery Branch, NIDCD, Bethesda, MD 20892, USA.
authors.forename: Carter
authors.initials: C
authors.lastname: Van Waes
authors.affiliation: Laboratory of Molecular Cardiology, NHLBI, Bethesda, MD 20814, USA.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Adelstein
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002465:Cell Movement / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004195:Disease Models, Animal; D018450:Disease Progression; D017353:Gene Deletion; D015870:Gene Expression; D014644:Genetic Variation; D005838:Genotype; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000473:pathology; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D009092:Mucous Membrane / Q000378:metabolism / Q000473:pathology; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D024507:Nonmuscle Myosin Type IIA / Q000235:genetics*; D015689:Oncogene Protein p21(ras) / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D015203:Reproducibility of Results; D014062:Tongue Neoplasms / Q000235:genetics* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D016159:Tumor Suppressor Protein p53; D024507:Nonmuscle Myosin Type IIA; D015689:Oncogene Protein p21(ras)