title: Induction of ROS generation and NF-κB activation in MARC-145 cells by a novel porcine reproductive and respiratory syndrome virus in Southwest of China isolate.
abstract: BACKGROUND
  Porcine reproductive and respiratory syndrome virus (PRRSV) is the cause of an economically important swine disease that has devastated the swine industry since the late 1980s. The aim of the present study was to investigate the interaction between reactive oxygen species (ROS) and NF-κB by PRRSV infection.
  
  
  RESULTS
  We isolated the local strain of PRRSV from southwest China, designated YN-2011, then sequenced and analyzed the genome. YN-2011 was then used to evaluate the interaction of ROS and NF-κB. In PRRSV infected MARC-145 cells, there was a time-dependent increase in ROS and Maleic Dialdehyde (MDA). Accordingly, NF-κB activation was also increased as PRRSV infection progressed. Degradation of IκB mRNA was detected late in PRRSV infection, and overexpression of the dominant negative form of IκBα significantly suppressed NF-κB induced by PRRSV.
  
  
  CONCLUSIONS
  The results indicate that the generation of ROS is involved in PRRSV replication and this progression is associated with the alteration in NF-κB activity induced by ROS. These results should extend our better understanding the interaction between PRRSV and host MARC-145 cells.
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, People's Republic of China. yanyulin333@163.com.|State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, People's Republic of China. yanyulin333@163.com.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan, People's Republic of China. 308816851@qq.com.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Xin
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, People's Republic of China. 348141549@qq.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, People's Republic of China. 176191413@qq.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, People's Republic of China. 656972802@qq.com.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Shao
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, People's Republic of China. 116333610@qq.com.
authors.forename: Yating
authors.initials: Y
authors.lastname: Zang
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, People's Republic of China. rcl80@163.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, People's Republic of China. 448913965@qq.com.
authors.forename: Yongke
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, People's Republic of China. gaohongping@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
mesh_terms: D000818:Animals; D002460:Cell Line; D002681:China / Q000453:epidemiology; D005786:Gene Expression Regulation / Q000502:physiology; D016679:Genome, Viral; D000882:Haplorhini; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013552:Swine
chemical_list: D016328:NF-kappa B; D017382:Reactive Oxygen Species