title: Phosphoketolase pathway engineering for carbon-efficient biocatalysis.
abstract: Recent advances in metabolic engineering have facilitated the development of microbial biocatalysts capable of producing an array of bio-products, ranging from fuels to drug molecules. These bio-products are commonly generated through an acetyl-CoA intermediate, which serves as a key precursor in the biological conversion of carbon substrates. Conventional biocatalytic upgrading strategies proceeding through this route are limited by low carbon efficiencies, in large part due to carbon losses associated with pyruvate decarboxylation to acetyl-CoA. Bypass of pyruvate decarboxylation offers a means to dramatically enhance carbon yields and, in turn, bioprocess economics. Herein, we discuss recent advances and prospects for employing the phosphoketolase pathway for direct biosynthesis of acetyl-CoA from carbon substrates, and phosphoketolase-based metabolic engineering strategies for carbon efficient biocatalysis.
authors.affiliation: National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, United States.
authors.forename: Calvin Andrew
authors.initials: CA
authors.lastname: Henard
authors.affiliation: National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, United States.
authors.forename: Emily Frances
authors.initials: EF
authors.lastname: Freed
authors.affiliation: National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, United States. Electronic address: Michael.Guarnieri@nrel.gov.
authors.forename: Michael Thomas
authors.initials: MT
authors.lastname: Guarnieri
mesh_terms: D000446:Aldehyde-Lyases / Q000378:metabolism*; D055162:Biocatalysis; D050260:Carbohydrate Metabolism; D002241:Carbohydrates; D002244:Carbon / Q000378:metabolism*; D060847:Metabolic Engineering
chemical_list: D002241:Carbohydrates; D002244:Carbon; D000446:Aldehyde-Lyases; C022367:phosphoketolase