title: Genome Engineering Using Adeno-associated Virus: Basic and Clinical Research Applications.
abstract: In addition to their broad potential for therapeutic gene delivery, adeno-associated virus (AAV) vectors possess the innate ability to stimulate homologous recombination in mammalian cells at high efficiencies. This process--referred to as AAV-mediated gene targeting--has enabled the introduction of a diverse array of genomic modifications both in vitro and in vivo. With the recent emergence of targeted nucleases, AAV-mediated genome engineering is poised for clinical translation. Here, we review key properties of AAV vectors that underscore its unique utility in genome editing. We highlight the broad range of genome engineering applications facilitated by this technology and discuss the strong potential for unifying AAV with targeted nucleases for next-generation gene therapy.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Berkeley, California, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Gaj
authors.affiliation: Department of Bioengineering, University of California, Berkeley, California, USA.
authors.forename: Benjamin E
authors.initials: BE
authors.lastname: Epstein
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Berkeley, California, USA.|Department of Bioengineering, University of California, Berkeley, California, USA.|Department of Cell and Molecular Biology, University of California, Berkeley, California, USA.|Helen Wills Neuroscience Institute, University of California, Berkeley, California, USA.
authors.forename: David V
authors.initials: DV
authors.lastname: Schaffer
mesh_terms: D000818:Animals; D000229:Dependovirus / Q000235:genetics* / Q000502:physiology; D004720:Endonucleases / Q000378:metabolism; D000072669:Gene Editing; D018390:Gene Targeting / Q000379:methods; D018014:Gene Transfer Techniques; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics*; D016678:Genome; D023281:Genomics / Q000379:methods; D006801:Humans; D012106:Research; D016662:Virus Integration
chemical_list: D004720:Endonucleases