title: [Application of molecular chaperones to soluble expression of e23sFv/His fusion proteins].
abstract: OBJECTIVE
  To explore the feasibility of applying molecular chaperones to the soluble expression of e23sFv/His fusion proteins.
  
  
  METHODS
  The molecular chaperone plasmid pGro7 or pKJE7 was transformed into BL21 (DE3) competent cells together with the prokaryotic expression vector harboring His-tagged e23sFv. The soluble expression of e23sFv/His proteins was induced at 16 °C. The yield and antigen-binding activity of the soluble products were compared with those of the insoluble products conventionally purified from inclusion bodies.
  
  
  RESULTS
  Both the overall yield and the purification ratio of soluble e23sFv/His proteins were relatively lower. The binding affinity of the soluble products to immobilized HER2 was not superior to that of the insoluble products from inclusion bodies.
  
  
  CONCLUSIONS
  The molecular chaperone plasmids pGro7 and pKJE7 partially facilitate the soluble expression of e23sFv/His proteins, but both the yield and the purification ratio are still limited.
authors.affiliation: Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chang
authors.affiliation: Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Ouyang
authors.affiliation: Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Angang
authors.initials: A
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yan
mesh_terms: D007128:Immunoglobulin Fragments / Q000235:genetics*; D018832:Molecular Chaperones / Q000235:genetics*; D010957:Plasmids; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D012995:Solubility
chemical_list: D007128:Immunoglobulin Fragments; D018832:Molecular Chaperones; D018719:Erb-b2 Receptor Tyrosine Kinases; D011993:Recombinant Fusion Proteins; C055966:immunoglobulin Fv