title: Feasibility of a facile butanol bioproduction using planetary mill pretreatment.
abstract: A facile butanol bioproduction process was developed using planetary milling, and Pinus rigida wood waste as a model substrate for fermentable sugars. The use of planetary milling as the pretreatment eliminates the need for washing and transfer of the biomass prior to enzymatic hydrolysis. Moreover, using this pretreatment process resulted in the production of only 0.072 ± 0.003 g/L soluble phenolic compounds, a concentration that was not inhibitory towards Clostridium beijerinckii NCIMB 8052. As the milling was performed in a compatible buffer (50mM acetate, pH 4.8), the enzymatic hydrolysis step was initiated by simply adding the cellulase cocktail powder directly to pretreated biomass without washing the biomass or exchanging the buffer, resulting in a glucose yield of 31 g/L (84.02%). Fermentation of the hydrolysate samples by C. beijerinckii NCIMB 8052 gave slightly better butanol yields than cultures grown in a typical lab media (P2), with final concentrations of 6.91 and 6.66 g/L, respectively.
authors.affiliation: Korea Institute of Ceramic Engineering and Technology (KICET), 101, Soho-ro, Jinju-si, Gyeongsangnam-do 52851, Republic of Korea; Division of Chemical Engineering & Bio Engineering, Hanyang University, Seoul, Republic of Korea.
authors.forename: Jeong Heo
authors.initials: JH
authors.lastname: Kwon
authors.affiliation: School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.
authors.forename: Hyunsoo
authors.initials: H
authors.lastname: Kang
authors.affiliation: Division of Chemical Engineering & Bio Engineering, Hanyang University, Seoul, Republic of Korea.
authors.forename: Byoung-In
authors.initials: BI
authors.lastname: Sang
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology, Seoul, Republic of Korea.
authors.forename: Yunje
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Division of Chemical Engineering, Chonbuk National University, Jeonju, Jeonbuk, Republic of Korea.
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
authors.affiliation: School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Mitchell
authors.affiliation: Korea Institute of Ceramic Engineering and Technology (KICET), 101, Soho-ro, Jinju-si, Gyeongsangnam-do 52851, Republic of Korea. Electronic address: leejinh1@kicet.re.kr.
authors.forename: Jin Hyung
authors.initials: JH
authors.lastname: Lee
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D000440:Butanols / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis; D002480:Cellulase / Q000378:metabolism; D046970:Clostridium beijerinckii / Q000378:metabolism; D005240:Feasibility Studies; D005285:Fermentation; D006868:Hydrolysis; D028223:Pinus / Q000737:chemistry*; D012997:Solvents; D013997:Time Factors
chemical_list: D000440:Butanols; D002241:Carbohydrates; D012997:Solvents; D002480:Cellulase
keywords: Biobutanol; Biofuel; Enhanced bioconversion; Planetary mill; Pretreatment