title: Effects of fish oil replacement by vegetable oil blend on digestive enzymes and tissue histomorphology of European sea bass (Dicentrarchus labrax) juveniles.
abstract: The impact of replacing circa 70% fish oil (FO) by a vegetable oil (VO) blend (rapeseed, linseed, palm oils; 20:50:30) in diets for European sea bass juveniles (IBW 96 ± 0.8 g) was evaluated in terms of activities of digestive enzymes (amylase, lipase, alkaline phosphatase, trypsin and total alkaline proteases) in the anterior (AI) and posterior (PI) intestine and tissue morphology (pyloric caeca-PC, AI, PI, distal intestine-DI and liver). For that purpose, fish were fed the experimental diets for 36 days and then liver and intestine were sampled at 2, 6 and 24 h after the last meal. Alkaline protease characterization was also done in AI and PI at 6 h post-feeding. Dietary VO promoted higher alkaline phosphatase activity at 2 h post-feeding in the AI and at all sampling points in the PI. Total alkaline protease activity was higher at 6 h post-feeding in the PI of fish fed the FO diet. Identical number of bands was observed in zymograms of alkaline proteases of fish fed both diets. No alterations in the histomorphology of PC, AI, PI or DI were noticed in fish fed the VO diets, while in the liver a tendency towards increased hepatocyte vacuolization due to lipid accumulation was observed. Overall, and with the exception of a higher intestine alkaline phosphatase activity, 70% FO replacement by a VO blend in diets for European sea bass resulted in no distinctive alterations on the postprandial pattern of digestive enzyme activities and intestine histomorphology.
authors.affiliation: CIMAR/CIIMAR- Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal.|Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, Edifício FC4, 4169-007, Porto, Portugal.
authors.forename: Carolina
authors.initials: C
authors.lastname: Castro
authors.affiliation: CIMAR/CIIMAR- Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal. acouto@fc.up.pt.|Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, Edifício FC4, 4169-007, Porto, Portugal. acouto@fc.up.pt.
authors.forename: Ana
authors.initials: A
authors.lastname: Couto
authors.affiliation: CIMAR/CIIMAR- Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal.
authors.forename: Amalia
authors.initials: A
authors.lastname: Pérez-Jiménez
authors.affiliation: CIMAR/CIIMAR- Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal.
authors.forename: Cláudia R
authors.initials: CR
authors.lastname: Serra
authors.affiliation: CIMAR/CIIMAR- Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal.
authors.forename: Patricia
authors.initials: P
authors.lastname: Díaz-Rosales
authors.affiliation: IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.
authors.forename: Rui
authors.initials: R
authors.lastname: Fernandes
authors.affiliation: INRA, UR1067 Nutrition Metabolism Aquaculture, 64310, Saint-Pée-sur-Nivelle, France.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Corraze
authors.affiliation: INRA, UR1067 Nutrition Metabolism Aquaculture, 64310, Saint-Pée-sur-Nivelle, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Panserat
authors.affiliation: CIMAR/CIIMAR- Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal.|Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, Edifício FC4, 4169-007, Porto, Portugal.
authors.forename: Aires
authors.initials: A
authors.lastname: Oliva-Teles
mesh_terms: D000818:Animals; D001492:Bass; D004041:Dietary Fats / Q000494:pharmacology*; D005229:Fatty Acids, Monounsaturated; D005395:Fish Oils / Q000494:pharmacology; D029941:Fish Proteins / Q000378:metabolism*; D006867:Hydrolases / Q000378:metabolism*; D007422:Intestines / Q000033:anatomy & histology / Q000201:enzymology*; D008043:Linseed Oil / Q000494:pharmacology; D008099:Liver / Q000201:enzymology* / Q000473:pathology; D000073878:Palm Oil; D010938:Plant Oils / Q000494:pharmacology; D019518:Postprandial Period / Q000502:physiology; D000074262:Rapeseed Oil
chemical_list: D004041:Dietary Fats; D005229:Fatty Acids, Monounsaturated; D005395:Fish Oils; D029941:Fish Proteins; D010938:Plant Oils; D000074262:Rapeseed Oil; D000073878:Palm Oil; D008043:Linseed Oil; D006867:Hydrolases
keywords: European sea bass; Fish; Fish oil; Postprandial digestive enzyme; Tissue histology; Vegetable oil