title: Electronic Olfactory Sensor Based on A. mellifera Odorant-Binding Protein 14 on a Reduced Graphene Oxide Field-Effect Transistor.
abstract: An olfactory biosensor based on a reduced graphene oxide (rGO) field-effect transistor (FET), functionalized by the odorant-binding protein 14 (OBP14) from the honey bee (Apis mellifera) has been designed for the in situ and real-time monitoring of a broad spectrum of odorants in aqueous solutions known to be attractants for bees. The electrical measurements of the binding of all tested odorants are shown to follow the Langmuir model for ligand-receptor interactions. The results demonstrate that OBP14 is able to bind odorants even after immobilization on rGO and can discriminate between ligands binding within a range of dissociation constants from K(d)=4 μM to K(d)=3.3 mM. The strongest ligands, such as homovanillic acid, eugenol, and methyl vanillate all contain a hydroxy group which is apparently important for the strong interaction with the protein.
authors.affiliation: BioSensor Technologies, Austrian Institute of Technology, Vienna (Austria).|Centre for Biomimetic Sensor Science, Nanyang Technological University, Singapore 637371 (Singapore).
authors.forename: Melanie
authors.initials: M
authors.lastname: Larisika
authors.affiliation: BioSensor Technologies, Austrian Institute of Technology, Vienna (Austria).|Center for Electrochemical Surface Technology, Wiener Neustadt (Austria).
authors.forename: Caroline
authors.initials: C
authors.lastname: Kotlowski
authors.affiliation: BioSensor Technologies, Austrian Institute of Technology, Vienna (Austria).
authors.forename: Christoph
authors.initials: C
authors.lastname: Steininger
authors.affiliation: Department of Biology of Agriculture, Food and Environment, University of Pisa (Italy).
authors.forename: Rosa
authors.initials: R
authors.lastname: Mastrogiacomo
authors.affiliation: Department of Biology of Agriculture, Food and Environment, University of Pisa (Italy).
authors.forename: Paolo
authors.initials: P
authors.lastname: Pelosi
authors.affiliation: Buesgen-Institute, Dept. of Forest Zoology and Forest Conservation, Goettingen (Germany).
authors.forename: Stefan
authors.initials: S
authors.lastname: Schütz
authors.affiliation: Michigan State University, Chemical Engineering & Materials Science (USA).
authors.forename: Serban F
authors.initials: SF
authors.lastname: Peteu
authors.affiliation: Center for Electrochemical Surface Technology, Wiener Neustadt (Austria).
authors.forename: Christoph
authors.initials: C
authors.lastname: Kleber
authors.affiliation: BioSensor Technologies, Austrian Institute of Technology, Vienna (Austria).|Center for Electrochemical Surface Technology, Wiener Neustadt (Austria).
authors.forename: Ciril
authors.initials: C
authors.lastname: Reiner-Rozman
authors.affiliation: BioSensor Technologies, Austrian Institute of Technology, Vienna (Austria).|Centre for Biomimetic Sensor Science, Nanyang Technological University, Singapore 637371 (Singapore).|Center for Electrochemical Surface Technology, Wiener Neustadt (Austria).
authors.forename: Christoph
authors.initials: C
authors.lastname: Nowak
authors.affiliation: BioSensor Technologies, Austrian Institute of Technology, Vienna (Austria). wolfgang.knoll@ait.ac.at.|Centre for Biomimetic Sensor Science, Nanyang Technological University, Singapore 637371 (Singapore). wolfgang.knoll@ait.ac.at.|Center for Electrochemical Surface Technology, Wiener Neustadt (Austria). wolfgang.knoll@ait.ac.at.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Knoll
mesh_terms: D000818:Animals; D001516:Bees / Q000737:chemistry*; D015374:Biosensing Techniques; D004583:Electrons; D006108:Graphite / Q000737:chemistry*; D009812:Odorants / Q000032:analysis*; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D018035:Receptors, Odorant / Q000737:chemistry*; D014173:Transistors, Electronic
chemical_list: D010087:Oxides; D018035:Receptors, Odorant; C045648:odorant-binding protein; D006108:Graphite
keywords: biosensors; immobilization; odorant-binding protein; olfaction; reduced graphene oxide