title: SpBADH of the halophyte Sesuvium portulacastrum strongly confers drought tolerance through ROS scavenging in transgenic Arabidopsis.
abstract: Glycine betaine (GB) accumulation is involved in abiotic stress. However, it is not known whether BADH, the key enzyme of GB synthesis, utilizes the antioxidant system to confer drought stress tolerance. In this study, a novel member of the ALDH10 gene family, SpBADH, was isolated from Sesuvium portulacastrum. The expression of this gene was up-regulated by NaCl, PEG6000, H2O2, ABA and high temperature in S. portulacastrum. SpBADH overexpression in Arabidopsis resulted in higher BADH activity and GB content and might increase tolerance to drought/osmotic stresses, specifically strong tolerance to drought stress. Transgenic lines exhibited lower MDA and H2O2 contents but higher proline, POD, SOD and CAT contents than the wild type under drought and osmotic stresses. SpBADH overexpression in Arabidopsis also enhanced the expression of ROS-related genes including AtSOD, AtPOD, AtCAT, AtAPX and Atpsb under drought and osmotic stresses. Thus, SpBADH increases plant tolerance to drought or osmotic stresses by reducing H2O2, increasing proline, and activating antioxidative enzymes to improve ROS scavenging.
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences &Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 570711, China; Guizhou Institute of Subtropics Crops, Xingyi 562400, China.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Yang
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences &Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 570711, China; College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences &Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 570711, China; Agricultural College of Hainan University, 571104 Haikou, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Fan
authors.affiliation: Guizhou Institute of Subtropics Crops, Xingyi 562400, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences &Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 570711, China.
authors.forename: Longbin
authors.initials: L
authors.lastname: Shen
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences &Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 570711, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences &Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 570711, China.
authors.forename: Ruimei
authors.initials: R
authors.lastname: Li
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences &Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 570711, China.
authors.forename: Shaoping
authors.initials: S
authors.lastname: Fu
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences &Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 570711, China.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Duan
authors.affiliation: Agricultural College of Hainan University, 571104 Haikou, China. Electronic address: huxinwen@hainu.edu.cn.
authors.forename: Xinwen
authors.initials: X
authors.lastname: Hu
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences &Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 570711, China. Electronic address: jianchunguoh@163.com.
authors.forename: Jianchun
authors.initials: J
authors.lastname: Guo
mesh_terms: D000222:Adaptation, Physiological; D035722:Aizoaceae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D001622:Betaine / Q000378:metabolism; D050728:Betaine-Aldehyde Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D055864:Droughts; D017343:Genes, Plant; D009997:Osmotic Pressure; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D001622:Betaine; D002374:Catalase; D013482:Superoxide Dismutase; D050728:Betaine-Aldehyde Dehydrogenase
keywords: Betaine aldehyde dehydrogenase (BADH); Drought stress; Environmental stress; Glycine betaine; Reactive oxygen species (ROS); Sesuvium portulacastrum; Transgenic Arabidopsis