title: Cerebral Area Differential Redox Response of Neonatal Rats to Selenite-Induced Oxidative Stress and to Concurrent Administration of Highbush Blueberry Leaf Polyphenols.
abstract: Our goal was to delineate the mechanisms of selenite-induced oxidative stress in neonatal rats and investigate the potential of blueberry leaf polyphenols to counteract the induced stress. Vaccinium corymbosum leaf decoction (BLD) was analyzed by UPLC-MS and LC-DAD, along with its in vitro antioxidant activity (DPPH radical scavenging, FRAP, ferrous chelation). Newborn suckling Wistar rats were randomly divided into three groups: 'Se' and 'SeBLD' received 20 μmol Na2SeO3/kg BW subcutaneously (PN day 10); 'SeBLD' received 100 mg dry BLD/kg BW intraperitoneally (PN11 and 12) and Group 'C' received normal saline. Βiochemical analysis revealed tissue-specific effects of selenite. Brain as a whole was more resistant to selenite toxicity in comparison to liver; midbrain and cerebellum were in general not affected, but cortex was moderately disturbed. Liver lipid peroxidation, GSH, SOD, CAT, GPx were significantly affected, whereas proteolytic activity was not. BLD, which is rich in chlorogenic acid and flavonols (especially quercetin derivatives), exerted significant antioxidant protective effects in all regions. In conclusion, we provide for the first time an insight to the neonatal rat cerebral and liver redox response against a toxic selenite dose and blueberry leaf polyphenols.
authors.affiliation: Laboratory of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, University of Patras, 26504, Patras, Greece.
authors.forename: Anastasia-Varvara
authors.initials: AV
authors.lastname: Ferlemi
authors.affiliation: Laboratory of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, University of Patras, 26504, Patras, Greece.
authors.forename: Penelope G
authors.initials: PG
authors.lastname: Mermigki
authors.affiliation: Department of Ophthalmology, Medical School, University of Patras, 26504, Patras, Greece.
authors.forename: Olga E
authors.initials: OE
authors.lastname: Makri
authors.affiliation: Bioanalytical Department, GAIA Research Center, The Goulandris Natural History Museum, 14562, Kifissia, Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Anagnostopoulos
authors.affiliation: Laboratory of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, University of Patras, 26504, Patras, Greece.|Bioanalytical Department, GAIA Research Center, The Goulandris Natural History Museum, 14562, Kifissia, Greece.
authors.forename: Nikolaos S
authors.initials: NS
authors.lastname: Koulakiotis
authors.affiliation: Laboratory of Human and Animal Physiology, Department of Biology, University of Patras, 26504, Patras, Greece.
authors.forename: Marigoula
authors.initials: M
authors.lastname: Margarity
authors.affiliation: Bioanalytical Department, GAIA Research Center, The Goulandris Natural History Museum, 14562, Kifissia, Greece.|Department of Pharmacology, Medical School, University of Athens, 11527, Athens, Greece.
authors.forename: Anthony
authors.initials: A
authors.lastname: Tsarbopoulos
authors.affiliation: Department of Ophthalmology, Medical School, University of Patras, 26504, Patras, Greece.
authors.forename: Constantinos D
authors.initials: CD
authors.lastname: Georgakopoulos
authors.affiliation: Laboratory of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, University of Patras, 26504, Patras, Greece. flam@upatras.gr.
authors.forename: Fotini N
authors.initials: FN
authors.lastname: Lamari
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D029798:Blueberry Plants / Q000737:chemistry*; D001923:Brain Chemistry / Q000187:drug effects*; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D020887:Selenious Acid / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols; D020887:Selenious Acid
keywords: Blueberry leaf polyphenols; Calpains; Cerebral areas; Cortex vulnerability; Selenite-induced oxidative stress; Vaccinium corymbosum