title: ShRNA-mediated silencing of the Ndc80 gene suppress cell proliferation and affected hepatitis B virus-related hepatocellular carcinoma.
abstract: BACKGROUND
  Hepatocellular carcinoma (HCC) is one of the most common and lethal malignancies in the world, and hepatitis B virus (HBV) has been well established to cause HCC. Ndc80 complex is a conserved mitotic regulator dedicated to ensuring faithful chromosome segregation and plays an important role in inducing tumor formation. However, its role in HCC caused by HBV infection remains unclear.
  
  
  METHODS
  Immunohistochemistry (IHC), Western blot (WB), and real-time qRT-PCR were used to measure the expression of Ndc80 in HBV-related HCC tissues. Ndc80-specific short hairpin RNA (shRNA) was used to knock-down Ndc80 expression in the hepatoma cell line HeG2 and HepG2.2.15, which is stable transcribed with HBV genome. Furthermore, the effect of Ndc80 on cellular proliferation and growth were examined, respectively.
  
  
  RESULTS
  The expression level of Ndc80 was remarkably up-regulated in HBV-related HCC tissues. Down-regulation of Ndc80 expression suppressed HBV replication. With cell counting and the MTS assay, cellular proliferation and growth of Ndc80 knocking-down cell line was shown to be effectively restrained.
  
  
  CONCLUSIONS
  This study suggests that Ndc80 may play an important role in the process of HBV-related HCC, and that it may be a potential biological treatment target in the future.
authors.affiliation: Department of general surgery, the third affiliated hospital, Sun Yat-Sen University, Guangzhou 510530, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of general surgery, the third affiliated hospital, Sun Yat-Sen University, Guangzhou 510530, China.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Department of general surgery, the third affiliated hospital, Sun Yat-Sen University, Guangzhou 510530, China.
authors.forename: Kunpeng
authors.initials: K
authors.lastname: Hu
authors.affiliation: Department of general surgery, the third affiliated hospital, Sun Yat-Sen University, Guangzhou 510530, China.
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of general surgery, the third affiliated hospital, Sun Yat-Sen University, Guangzhou 510530, China.
authors.forename: Shilei
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of general surgery, the third affiliated hospital, Sun Yat-Sen University, Guangzhou 510530, China.
authors.forename: Qinliang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of liver transplantation, the third affiliated hospital, Sun Yat-Sen University, Guangzhou 510530, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of ultrasound, the third affiliated hospital, Sun Yat-Sen University, Guangzhou 510530, China. Electronic address: renjie150421@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Ren
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000821:virology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D003598:Cytoskeletal Proteins; D015536:Down-Regulation; D020868:Gene Silencing; D006515:Hepatitis B virus / Q000472:pathogenicity*; D006801:Humans; D008113:Liver Neoplasms / Q000821:virology*; D009687:Nuclear Proteins / Q000235:genetics*; D034741:RNA, Small Interfering; D015854:Up-Regulation; D014779:Virus Replication / Q000235:genetics
chemical_list: D003598:Cytoskeletal Proteins; C120529:NDC80 protein, human; D009687:Nuclear Proteins; D034741:RNA, Small Interfering