title: Ultraviolet Radiation-Elicited Enhancement of Isoflavonoid Accumulation, Biosynthetic Gene Expression, and Antioxidant Activity in Astragalus membranaceus Hairy Root Cultures.
abstract: In this work, Astragalus membranaceus hairy root cultures (AMHRCs) were exposed to ultraviolet radiation (UV-A, UV-B, and UV-C) for promoting isoflavonoid accumulation. The optimum enhancement for isoflavonoid production was achieved in 34-day-old AMHRCs elicited by 86.4 kJ/m(2) of UV-B. The resulting isoflavonoid yield was 533.54 ± 13.61 μg/g dry weight (DW), which was 2.29-fold higher relative to control (232.93 ± 3.08 μg/g DW). UV-B up-regulated the transcriptional expressions of all investigated genes involved in isoflavonoid biosynthetic pathway. PAL and C4H were found to be two potential key genes that controlled isoflavonoid biosynthesis. Moreover, a significant increase was noted in antioxidant activity of extracts from UV-B-elicited AMHRCs (IC50 values = 0.85 and 1.08 mg/mL) in comparison with control (1.38 and 1.71 mg/mL). Overall, this study offered a feasible elicitation strategy to enhance isoflavonoid accumulation in AMHRCs and also provided a basis for metabolic engineering of isoflavonoid biosynthesis in the future.
authors.forename: Jiao
authors.initials: J
authors.lastname: Jiao
authors.affiliation: Collaborative Innovation Center for Development and Utilization of Forest Resources , Harbin, Heilongjiang 150040, People's Republic of China.
authors.forename: Qing-Yan
authors.initials: QY
authors.lastname: Gai
authors.affiliation: Collaborative Innovation Center for Development and Utilization of Forest Resources , Harbin, Heilongjiang 150040, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Collaborative Innovation Center for Development and Utilization of Forest Resources , Harbin, Heilongjiang 150040, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Luo
authors.affiliation: Collaborative Innovation Center for Development and Utilization of Forest Resources , Harbin, Heilongjiang 150040, People's Republic of China.
authors.forename: Cheng-Bo
authors.initials: CB
authors.lastname: Gu
authors.affiliation: Collaborative Innovation Center for Development and Utilization of Forest Resources , Harbin, Heilongjiang 150040, People's Republic of China.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Fu
authors.affiliation: School of Pharmaceutical, Heilongjiang University of Chinese Medicine , Harbin, Heilongjiang 150040, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D027885:Astragalus propinquus / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005419:Flavonoids / Q000096:biosynthesis* / Q000235:genetics* / Q000494:pharmacology; D015870:Gene Expression / Q000528:radiation effects*; D018517:Plant Roots / Q000378:metabolism / Q000528:radiation effects; D046509:Tissue Culture Techniques; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D005419:Flavonoids
keywords: Astragalus membranaceus; antioxidant activity; gene expression; hairy root cultures; isoflavonoid biosynthesis; ultraviolet elicitation