title: Hydrolyzed whey protein prevents the development of food allergy to β-lactoglobulin in sensitized mice.
abstract: Food allergy is an adverse immune response to dietary proteins. Hydrolysates are frequently used for children with milk allergy. However, hydrolysates effects afterwards are poorly studied. The aim of this study was to investigate the immunological consequences of hydrolyzed whey protein in allergic mice. For that, we developed a novel model of food allergy in BALB/c mice sensitized with alum-adsorbed β-lactoglobulin. These mice were orally challenged with either whey protein or whey hydrolysate. Whey-challenged mice had elevated levels of specific IgE and lost weight. They also presented gut inflammation, enhanced levels of SIgA and IL-5 as well as decreased production of IL-4 and IL-10 in the intestinal mucosa. Conversely, mice challenged with hydrolyzate maintained normal levels of IgE, IL-4 and IL-5 and showed no sign of gut inflammation probably due to increased IL-12 production in the gut. Thus, consumption of hydrolysate prevented the development of clinical signs of food allergy in mice.
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Brazil. Electronic address: hosttana@gmail.com.
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Gomes-Santos
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Brazil.
authors.forename: Roberta Cristelli
authors.initials: RC
authors.lastname: Fonseca
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Brazil.
authors.forename: Luisa
authors.initials: L
authors.lastname: Lemos
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Brazil.
authors.forename: Daniela Silva
authors.initials: DS
authors.lastname: Reis
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Brazil.
authors.forename: Thaís Garcias
authors.initials: TG
authors.lastname: Moreira
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Brazil.
authors.forename: Adna Luciana
authors.initials: AL
authors.lastname: Souza
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Brazil.
authors.forename: Mauro Ramalho
authors.initials: MR
authors.lastname: Silva
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Brazil.
authors.forename: Marialice Pinto Coelho
authors.initials: MP
authors.lastname: Silvestre
authors.affiliation: Departamento de Morfologia, Universidade Federal de Minas Gerais, Brazil.
authors.forename: Denise Carmona
authors.initials: DC
authors.lastname: Cara
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Brazil.
authors.forename: Ana Maria Caetano
authors.initials: AM
authors.lastname: Faria
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005512:Food Hypersensitivity / Q000276:immunology; D007071:Immunoglobulin A, Secretory / Q000276:immunology / Q000378:metabolism; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation / Q000276:immunology; D016753:Interleukin-10 / Q000378:metabolism; D053765:Interleukin-12 Subunit p35 / Q000096:biosynthesis / Q000276:immunology; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology*; D007782:Lactoglobulins / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016269:Milk Hypersensitivity / Q000276:immunology*; D011492:Protein Hydrolysates / Q000008:administration & dosage / Q000276:immunology*; D000067796:Whey / Q000276:immunology; D000067816:Whey Proteins / Q000276:immunology* / Q000494:pharmacology
chemical_list: C556062:IL10 protein, mouse; C508859:Il12a protein, mouse; D007071:Immunoglobulin A, Secretory; D053765:Interleukin-12 Subunit p35; D015848:Interleukin-5; D007782:Lactoglobulins; D011492:Protein Hydrolysates; D000067816:Whey Proteins; D016753:Interleukin-10; D015847:Interleukin-4; D007073:Immunoglobulin E
keywords: Cytokine; Food allergy; Hydrolysate; SIgA; Whey protein; β-Lactoglobulin