title: Aflatoxin B1 Detection Using a Highly-Sensitive Molecularly-Imprinted Electrochemical Sensor Based on an Electropolymerized Metal Organic Framework.
abstract: A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through π-π interactions between AFB1 molecules and aniline moities. The performance of the developed sensor for the detection of AFB1 was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as a redox probe, the electron transfer rate increasing when the concentration of AFB1 increases, due to a p-doping effect. The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM. Compared to the non-imprinted sensor, the imprinting factor was found to be 10. Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China. mjjiang@mails.ccnu.edu.cn.
authors.forename: Mengjuan
authors.initials: M
authors.lastname: Jiang
authors.affiliation: University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, La Doua Street, 5, Villeurbanne 69100, France. mohamed_braiek@yahoo.fr.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Braiek
authors.affiliation: University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, La Doua Street, 5, Villeurbanne 69100, France. florea.ancas@yahoo.com.
authors.forename: Anca
authors.initials: A
authors.lastname: Florea
authors.affiliation: University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, La Doua Street, 5, Villeurbanne 69100, France. amani.chrouda@yahoo.fr.
authors.forename: Amani
authors.initials: A
authors.lastname: Chrouda
authors.affiliation: University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, La Doua Street, 5, Villeurbanne 69100, France. carole.farre@univ-lyon1.fr.
authors.forename: Carole
authors.initials: C
authors.lastname: Farre
authors.affiliation: University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, La Doua Street, 5, Villeurbanne 69100, France. anne.bonhomme@isa-lyon.fr.
authors.forename: Anne
authors.initials: A
authors.lastname: Bonhomme
authors.affiliation: University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, La Doua Street, 5, Villeurbanne 69100, France. francois.bessueille@univ-lyon1.fr.
authors.forename: Francois
authors.initials: F
authors.lastname: Bessueille
authors.affiliation: University of Lyon, Laboratoire Hubert Curien, UMR 5516, Jean-Monnet University of Saint-Etienne, Saint-Etienne F-42023, France. francis.vocanson@univ-st-etienne.fr.
authors.forename: Francis
authors.initials: F
authors.lastname: Vocanson
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China. adzhang@mail.ccnu.edu.cn.
authors.forename: Aidong
authors.initials: A
authors.lastname: Zhang
authors.affiliation: University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, La Doua Street, 5, Villeurbanne 69100, France. nicole.jaffrezic@univ-lyon1.fr.
authors.forename: Nicole
authors.initials: N
authors.lastname: Jaffrezic-Renault
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D000814:Aniline Compounds / Q000032:analysis / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D000814:Aniline Compounds; D013438:Sulfhydryl Compounds; D006046:Gold; C064316:4-aminothiophenol; D016604:Aflatoxin B1; C023650:aniline
keywords: aflatoxin B1; electrochemical sensors; gold nanoparticles; metal organic framework; molecularly imprinted polymers