title: The effects of injectable calcium silicate-based composites with the Chinese herb on an osteogenic accelerator in vitro.
abstract: We aimed to investigate the physicochemical and biological effects of calcium silicate (CS)-based cements together with the Chinese medicine Xu Duan (XD) after seeding with human adipose-derived stem cells (hADSCs). Here, we fabricated CS-based substrates with different ratios of XD (0%, 5% and 10%) as bioactive and biodegradable biocomposites, subsequent to examining their respective effectiveness for bone repair. The setting time, the injectability, the mechanical properties measured by diametral tensile strength (DTS), the in vitro degradation determined by changes in the weight loss of the composites, the characteristic formation of bone-like apatite, and cell growth as well as osteogenesis protein and bone mineralization were comprehensively evaluated before and after immersion in simulated body fluid (SBF), respectively. At the end of testing, with regard to physicochemical effects, the CS-based substrate mixed with the 10% XD group showed significantly sound mechanical properties, an applicable setting time and injectability and the formation of a dense bone-like apatite layer. In terms of biological effects, the CS-based substrate with the 10% XD group showed a significant development of osteogenic activities with sound cell proliferation and higher alkaline phosphatase (ALP) activity, as well as indicating osteogenic differentiation, greater osteocalcin (OC) protein secretion and clearly calcified tissue mineralization. The present drug-release strategy with CS-based cements may pave the way for future alternative bone repair therapy.
authors.affiliation: Department of Sports Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan.
authors.forename: Nai-Jen
authors.initials: NJ
authors.lastname: Chang
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Chen
authors.forename: Den-En
authors.initials: DE
authors.lastname: Shieh
authors.forename: Hsin-Yuan
authors.initials: HY
authors.lastname: Fang
authors.forename: Ming-You
authors.initials: MY
authors.lastname: Shie
mesh_terms: D020341:Absorbable Implants; D001843:Bone Cements / Q000737:chemistry / Q000627:therapeutic use*; D017610:Calcium Compounds / Q000008:administration & dosage* / Q000737:chemistry; D002478:Cells, Cultured; D004343:Drug Implants / Q000008:administration & dosage* / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D007267:Injections; D008422:Materials Testing; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D017640:Silicates / Q000008:administration & dosage* / Q000737:chemistry; D033581:Stem Cell Transplantation / Q000379:methods*; D013718:Tensile Strength; D014783:Viscosity
chemical_list: D001843:Bone Cements; D017610:Calcium Compounds; D004343:Drug Implants; D004365:Drugs, Chinese Herbal; D017640:Silicates; C031293:calcium silicate