title: Comparison of inherently essential genes of Porphyromonas gingivalis identified in two transposon-sequencing libraries.
abstract: Porphyromonas gingivalis is a Gram-negative anaerobe and keystone periodontal pathogen. A mariner transposon insertion mutant library has recently been used to define 463 genes as putatively essential for the in vitro growth of P. gingivalis ATCC 33277 in planktonic culture (Library 1). We have independently generated a transposon insertion mutant library (Library 2) for the same P. gingivalis strain and herein compare genes that are putatively essential for in vitro growth in complex media, as defined by both libraries. In all, 281 genes (61%) identified by Library 1 were common to Library 2. Many of these common genes are involved in fundamentally important metabolic pathways, notably pyrimidine cycling as well as lipopolysaccharide, peptidoglycan, pantothenate and coenzyme A biosynthesis, and nicotinate and nicotinamide metabolism. Also in common are genes encoding heat-shock protein homologues, sigma factors, enzymes with proteolytic activity, and the majority of sec-related protein export genes. In addition to facilitating a better understanding of critical physiological processes, transposon-sequencing technology has the potential to identify novel strategies for the control of P. gingivalis infections. Those genes defined as essential by two independently generated TnSeq mutant libraries are likely to represent particularly attractive therapeutic targets.
authors.affiliation: Department of Microbiology and Immunology, University of Louisville, Louisville, KY, USA.|Department of Oral Immunology and Infectious Diseases, University of Louisville, Louisville, KY, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Hutcherson
authors.affiliation: Department of Oral Immunology and Infectious Diseases, University of Louisville, Louisville, KY, USA.
authors.forename: H
authors.initials: H
authors.lastname: Gogeneni
authors.affiliation: Department of Biology, University of Louisville, Louisville, KY, USA.
authors.forename: D
authors.initials: D
authors.lastname: Yoder-Himes
authors.affiliation: Center for Microbial Proteomics and Chemical Engineering, University of Washington, Seattle, WA, USA.
authors.forename: E L
authors.initials: EL
authors.lastname: Hendrickson
authors.affiliation: Center for Microbial Proteomics and Chemical Engineering, University of Washington, Seattle, WA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Hackett
authors.affiliation: Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.
authors.forename: M
authors.initials: M
authors.lastname: Whiteley
authors.affiliation: Department of Oral Immunology and Infectious Diseases, University of Louisville, Louisville, KY, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Lamont
authors.affiliation: Department of Oral Immunology and Infectious Diseases, University of Louisville, Louisville, KY, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Scott
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods; D004251:DNA Transposable Elements; D015723:Gene Library; D005798:Genes, Bacterial; D020043:Genes, Essential; D006360:Heat-Shock Proteins / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D008070:Lipopolysaccharides / Q000096:biosynthesis; D016254:Mutagenesis, Insertional; D009154:Mutation; D010510:Periodontal Diseases / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000235:genetics* / Q000254:growth & development; D011743:Pyrimidines / Q000378:metabolism; D012808:Sigma Factor / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D006360:Heat-Shock Proteins; D008070:Lipopolysaccharides; D011743:Pyrimidines; D012808:Sigma Factor; C030986:pyrimidine
keywords: KEGG; Porphyromonas gingivalis; TnSeq; cigarette smoking; essential genes; periodontal diseases