title: Newly identified RNAs of raspberry leaf blotch virus encoding a related group of proteins.
abstract: Members of the genus Emaravirus, including Raspberry leaf blotch virus (RLBV), are enveloped plant viruses with segmented genomes of negative-strand RNA, although the complete genome complement for any of these viruses is not yet clear. Currently, wheat mosaic virus has the largest emaravirus genome comprising eight RNAs. Previously, we identified five genomic RNAs for RLBV; here, we identify a further three RNAs (RNA6-8). RNA6-8 encode proteins that have clear homologies to one another, but not to any other emaravirus proteins. The proteins self-interacted in yeast two-hybrid and bimolecular fluorescence complementation (BiFC) experiments, and the P8 protein interacted with the virus nucleocapsid protein (P3) using BiFC. Expression of two of the proteins (P6 and P7) using potato virus X led to an increase in virus titre and symptom severity, suggesting that these proteins may play a role in RLBV pathogenicity; however, using two different tests, RNA silencing suppression activity was not detected for any of the RLBV proteins encoded by RNA2-8.
authors.affiliation: State Key laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.|Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha 410128, PR China.
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Lu
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: Wendy
authors.initials: W
authors.lastname: McGavin
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: Peter J A
authors.initials: PJA
authors.lastname: Cock
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK.
authors.forename: Esther
authors.initials: E
authors.lastname: Schnettler
authors.affiliation: State Key laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
authors.affiliation: State Key laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: Stuart
authors.initials: S
authors.lastname: MacFarlane
mesh_terms: D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D065856:Rubus / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins