title: In Vitro and In Vivo Studies on Protective Action of N-Phenethyl Caffeamide against Photodamage of Skin.
abstract: In our previous study, N-phenethyl caffeamide (K36) was proved to act as an antioxidant and an antiphotoaging agent by inhibiting type I procollagen degradation and stimulating collagen synthesis in human skin fibroblasts. In the present study, in vitro and in vivo experiments were conducted to investigate the mechanism of action and the antiinflammatory and antiphotoaging activity of K36. K36 reduced UVB-induced cyclooxygenase-2 (COX-2) and inducible nitric oxide synthases (iNOS) expression by regulating IκB and p-IκB expression. K36 also inhibited the nuclear translocation of NF-κB. Furthermore, the inhibition of mitogen-activated protein (MAP) kinases by K36 was attributed to the downregulation of COX-2. Topically applying K36 led to efficient antiwrinkle formation and reduced UVB-induced erythema and thickness of epidermis in hairless mice. In addition, K36 penetrated into the skin of hairless mice. Our findings show that K36 has significant beneficial effects on antioxidant, antiinflammatory, and antiphotoaging activity and suggest that K36 can be developed as an antiaging agent for cosmetic and skin care products.
authors.affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan; Department of Biotechnology, Asia University, Taichung 413, Taiwan.
authors.forename: Yueh-Hsiung
authors.initials: YH
authors.lastname: Kuo
authors.affiliation: Department of Cosmeceutics, China Medical University, Taichung 404, Taiwan.
authors.forename: Chien-Wen
authors.initials: CW
authors.lastname: Chen
authors.affiliation: Department of Cosmeceutics, China Medical University, Taichung 404, Taiwan.
authors.forename: Yin
authors.initials: Y
authors.lastname: Chu
authors.affiliation: Department of Cosmeceutics, China Medical University, Taichung 404, Taiwan.
authors.forename: Ping
authors.initials: P
authors.lastname: Lin
authors.affiliation: Department of Cosmeceutics, China Medical University, Taichung 404, Taiwan.
authors.forename: Hsiu-Mei
authors.initials: HM
authors.lastname: Chiang
mesh_terms: D000818:Animals; D002109:Caffeic Acids / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004817:Epidermis / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects; D013473:Sunscreening Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D002109:Caffeic Acids; D020672:I-kappa B Proteins; C549043:N-2-(4-bromophenyl) ethyl caffeamide; C000608552:N-phenethyl caffeamide; D016328:NF-kappa B; D013473:Sunscreening Agents; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2