title: A regenerative microchannel device for recording multiple single-unit action potentials in awake, ambulatory animals.
abstract: Despite significant advances in robotics, commercially advanced prosthetics provide only a small fraction of the functionality of the amputated limb that they are meant to replace. Peripheral nerve interfacing could provide a rich controlling link between the body and these advanced prosthetics in order to increase their overall utility. Here, we report on the development of a fully integrated regenerative microchannel interface with 30 microelectrodes and signal extraction capabilities enabling evaluation in an awake and ambulatory rat animal model. In vitro functional testing validated the capability of the microelectrodes to record neural signals similar in size and nature to those that occur in vivo. In vitro dorsal root ganglia cultures revealed striking cytocompatibility of the microchannel interface. Finally, in vivo, the microchannel interface was successfully used to record a multitude of single-unit action potentials through 63% of the integrated microelectrodes at the early time point of 3 weeks. This marks a significant advance in microchannel interfacing, demonstrating the capability of microchannels to be used for peripheral nerve interfacing.
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, 313 Ferst Drive, Atlanta, GA, 30332, USA.
authors.forename: Akhil
authors.initials: A
authors.lastname: Srinivasan
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, 313 Ferst Drive, Atlanta, GA, 30332, USA.
authors.forename: John
authors.initials: J
authors.lastname: Tipton
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, 313 Ferst Drive, Atlanta, GA, 30332, USA.
authors.forename: Mayank
authors.initials: M
authors.lastname: Tahilramani
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, 313 Ferst Drive, Atlanta, GA, 30332, USA.
authors.forename: Adel
authors.initials: A
authors.lastname: Kharbouch
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, 313 Ferst Drive, Atlanta, GA, 30332, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Gaupp
authors.affiliation: School of Electrical and Computer Engineering, Georgia Institute of Technology and Emory University School of Medicine, Atlanta, GA, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: Song
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, 313 Ferst Drive, Atlanta, GA, 30332, USA.
authors.forename: Poornima
authors.initials: P
authors.lastname: Venkataraman
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, 313 Ferst Drive, Atlanta, GA, 30332, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Falcone
authors.affiliation: Centre for Neuroprosthetics, School of Engineering, Institute of Microengineering and Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne, Switzerland.
authors.forename: Stéphanie P
authors.initials: SP
authors.lastname: Lacour
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, 313 Ferst Drive, Atlanta, GA, 30332, USA.
authors.forename: Garrett B
authors.initials: GB
authors.lastname: Stanley
authors.affiliation: Department of Cell Biology, Emory University School of Medicine, Atlanta, GA, USA.
authors.forename: Arthur W
authors.initials: AW
authors.lastname: English
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, 313 Ferst Drive, Atlanta, GA, 30332, USA.
authors.forename: Ravi V
authors.initials: RV
authors.lastname: Bellamkonda
mesh_terms: D000200:Action Potentials; D000669:Amplifiers, Electronic; D000818:Animals; D002478:Cells, Cultured; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods*; D005727:Ganglia, Spinal / Q000502:physiology; D008839:Microelectrodes; D010525:Peripheral Nerves / Q000502:physiology; D051381:Rats; D014851:Wakefulness
keywords: nerve conduction; nerve injury and regeneration; neural interfacing; neural recordings; regenerative neural interface