title: Metformin Protects Auditory Hair Cells from Gentamicin-Induced Toxicity in vitro.
abstract: Metformin is a commonly used antidiabetic drug. It has been shown that this drug activates the AMP-activated protein kinase, which inhibits downstream the mammalian target of rapamycin. In addition, several studies indicate that metformin reduces intracellular reactive oxygen species. Our data, using an in vitro rat model, indicate that metformin is able to protect auditory hair cells (HCs) from gentamicin-induced apoptotic cell death. Moreover, metformin has no toxic effect on spiral ganglion neuronal survival or outgrowth in vitro. These results suggest a protective effect of metformin on auditory HC survival in gentamicin-induced HC loss in vitro.
authors.affiliation: Department of Biomedicine, Head and Neck Surgery, University Hospital Basel, Basel, Switzerland.
authors.forename: Andrea
authors.initials: A
authors.lastname: Glutz
authors.forename: Katharina
authors.initials: K
authors.lastname: Leitmeyer
authors.forename: Cristian
authors.initials: C
authors.lastname: Setz
authors.forename: Yves
authors.initials: Y
authors.lastname: Brand
authors.forename: Daniel
authors.initials: D
authors.lastname: Bodmer
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D005839:Gentamicins / Q000633:toxicity*; D006198:Hair Cells, Auditory / Q000187:drug effects*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D066298:In Vitro Techniques; D008687:Metformin / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D011500:Protein Synthesis Inhibitors / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013136:Spiral Ganglion / Q000166:cytology
chemical_list: D005839:Gentamicins; D007004:Hypoglycemic Agents; D011500:Protein Synthesis Inhibitors; D008687:Metformin