title: Oxidative damage induced by copper in mouse primary hepatocytes by single-cell analysis.
abstract: Copper can disturb the intracellular redox balance, induce oxidative stress, and subsequently cause irreversible damage, leading to a variety of diseases. In the present study, mouse primary hepatocytes were chosen to elucidate the in vitro oxidative damage of short-term copper exposure (10-200 μM) by single-cell analysis. We evaluated the toxicity of copper by reactive oxygen species (ROS), glutathione (GSH), and oxidative DNA damage at the single-cell level. Oxidative damage induced by copper was verified by the morphological changes, persistent elevations of excessive ROS and malondialdehyde (MDA), a decrease in GSH level, and the oxidative DNA damage. Furthermore, the average ROS generation, GSH consumption, and the indicators in DNA damage did not significantly change at relatively low concentrations (10 or 50 μM), but we can find the alterations of parameters in some single cells clearly. Emphasis on the analysis of single cells is conducive to gain a better understanding on the toxicity of copper. This study will also complement studies on the environmental risk assessment of copper pollution.
authors.affiliation: School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong University, 27# Shanda South Road, Jinan, 250100, People's Republic of China.
authors.forename: Mingyang
authors.initials: M
authors.lastname: Jing
authors.affiliation: School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong University, 27# Shanda South Road, Jinan, 250100, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong University, 27# Shanda South Road, Jinan, 250100, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University, 27 Shandanan Road, Jinan, 250100, People's Republic of China.
authors.forename: Yunxing
authors.initials: Y
authors.lastname: Yan
authors.affiliation: School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong University, 27# Shanda South Road, Jinan, 250100, People's Republic of China.
authors.forename: Wenbao
authors.initials: W
authors.lastname: Yan
authors.affiliation: School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong University, 27# Shanda South Road, Jinan, 250100, People's Republic of China. rutaoliu@sdu.edu.cn.
authors.forename: Rutao
authors.initials: R
authors.lastname: Liu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003300:Copper / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D059010:Single-Cell Analysis
chemical_list: D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D003300:Copper; D005978:Glutathione
keywords: Copper; DNA damage; Glutathione; Mouse primary hepatocytes; Oxidative damage; Reactive oxygen species; Single-cell analysis