title: Rapid Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) by the Vitek MS Saramis system.
abstract: A rapid, sensitive, and accurate Vitek MS assay was developed to distinguish clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) from clinical isolates of methicillin-sensitive Staphylococcus aureus (MSSA) by developing an in-house knowledgebase of SuperSpectra. Three unique peaks, including peaks at 2305.6 and 3007.3 Da specific to MRSA, and 6816.7 Da specific to MSSA, were selected for differentiating MRSA and MSSA. This assay accurately identified 84 and 91% of clinical MRSA and MSSA strains out of the total 142 clinically acquired S. aureus strains that were tested. This method will greatly improve the efficiency of single clinical sample identification of MRSA, thereby facilitating a reduction in the transmission of MRSA in clinical settings.
authors.affiliation: College of Pharmacy Science, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Weiguang
authors.initials: W
authors.lastname: Shan
authors.affiliation: College of Pharmacy Science, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Jiaping
authors.initials: J
authors.lastname: Li
authors.affiliation: Second Affiliated Hospital of Zhejiang University, Hangzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Second Affiliated Hospital of Zhejiang University, Hangzhou, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Second Affiliated Hospital of Zhejiang University, Hangzhou, China.
authors.forename: Danxia
authors.initials: D
authors.lastname: Gu
authors.affiliation: Second Affiliated Hospital of Zhejiang University, Hangzhou, China. brigitte_zx@163.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology*; D013997:Time Factors