title: Repetitive Immunoassay with a Surface Acoustic Wave Device and a Highly Stable Protein Monolayer for On-Site Monitoring of Airborne Dust Mite Allergens.
abstract: This work describes a sensor to be incorporated into the on-site monitoring system of airborne house dust mite (HDM) allergens. A surface acoustic wave (SAW) device was combined with self-assembled monolayers of a highly stable antibody capture protein on the SAW surface that have high resistance to pH change. A sandwich assay was used to measure a HDM allergen, Der f 1 derived from Dermatophagoides farinae. Capture antibodies were cross-linked to a protein G based capture layer (ORLA85) on the sensor surface, thereby only Der f 1 and detection antibodies were regenerated by changing pH, resulting in fast repetition of the measurement. The sensor was characterized through 10 repetitive measurements of Der f 1, which demonstrated high reproducibility of the sensor with the coefficient of variation of 5.6%. The limit of detection (LOD) of the sensor was 6.1 ng·mL(-1), encompassing the standard (20 ng·mL(-1)) set by the World Health Organization. Negligible sensor outputs were observed for five different major allergens including other HDM allergens which tend to have cross-reactivity to Der f 1 and their mixtures with Der f 1. Finally, the sensor lifetime was evaluated by conducting three measurements per day, and the sensor output did not substantially change for 4 days. These characteristics make the SAW immunosensor a promising candidate for incorporation into on-site allergen monitoring systems.
authors.affiliation: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University , 2-3-10 Kanda-Surugadai , Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Toma
authors.affiliation: Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Miki
authors.affiliation: Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors.forename: Chisato
authors.initials: C
authors.lastname: Kishikawa
authors.affiliation: Japan Radio Co. Ltd. , Saitama 356-8510, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Yoshimura
authors.affiliation: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University , 2-3-10 Kanda-Surugadai , Chiyoda-ku, Tokyo 101-0062, Japan.|Japan Society for the Promotion of Science , Tokyo 102-0083, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Miyajima
authors.affiliation: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University , 2-3-10 Kanda-Surugadai , Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Arakawa
authors.affiliation: Japan Radio Co. Ltd. , Saitama 356-8510, Japan.|OJ-Bio Ltd. , NE1 4EP Newcastle upon Tyne, United Kingdom.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yatsuda
authors.affiliation: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University , 2-3-10 Kanda-Surugadai , Chiyoda-ku, Tokyo 101-0062, Japan.|Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Mitsubayashi
mesh_terms: D016902:Air Pollution, Indoor / Q000032:analysis*; D000485:Allergens / Q000032:analysis* / Q000276:immunology; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000032:analysis* / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000276:immunology; D002415:Cats; D004391:Dust / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation; D013016:Sound; D013499:Surface Properties
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D001426:Bacterial Proteins; D004391:Dust; C034284:IgG Fc-binding protein, Streptococcus