title: Synergistic effect of Pseudomonas putida and Bacillus amyloliquefaciens ameliorates drought stress in chickpea (Cicer arietinum L.).
abstract: Two plant growth promoting rhizobacteria (PGPR) Pseudomonas putida NBRIRA and Bacillus amyloliquefaciens NBRISN13 with ability to tolerate abiotic stress along with multiple PGP traits like ACC deaminase activity, minerals solubilisation, hormones production, biofilm formation, siderophore activity were evaluated for their synergistic effect to ameliorate drought stress in chickpea. Earlier we have reported both the strains individually for their PGP attributes and stress amelioration in host plants. The present study explains in detail the possibilities and benefits of utilizing these 2 PGPR in consortium for improving the chickpea growth under control and drought stressed condition. In vitro results clearly demonstrate that both the PGPR strains are compatible to each other and their synergistic growth enhances the PGP attributes. Greenhouse experiments were conducted to evaluate the effect of inoculation of both strains individually and consortia in drought tolerant and sensitive cultivars (BG362 and P1003). The growth parameters were observed significantly higher in consortium as compared to individual PGPR. Colonization of both PGPR in chickpea rhizosphere has been visualized by using gfp labeling. Apart from growth parameters, defense enzymes, soil enzymes and microbial diversity were significantly modulated in individually PGPR and in consortia inoculated plants. Negative effects of drought stress has been ameliorated and apparently seen by higher biomass and reversal of stress indicators in chickpea cultivars treated with PGPR individually or in consortia. Findings from the present study demonstrate that synergistic application has better potential to improve plant growth promotion under drought stress conditions.
authors.affiliation: a Division of Plant Microbe Interactions; CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.affiliation: a Division of Plant Microbe Interactions; CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , India.
authors.forename: Sankalp
authors.initials: S
authors.lastname: Mishra
authors.affiliation: a Division of Plant Microbe Interactions; CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , India.
authors.forename: Vijaykant
authors.initials: V
authors.lastname: Dixit
authors.affiliation: a Division of Plant Microbe Interactions; CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.affiliation: a Division of Plant Microbe Interactions; CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , India.
authors.forename: Lalit
authors.initials: L
authors.lastname: Agarwal
authors.affiliation: a Division of Plant Microbe Interactions; CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , India.
authors.forename: Puneet Singh
authors.initials: PS
authors.lastname: Chauhan
authors.affiliation: a Division of Plant Microbe Interactions; CSIR-National Botanical Research Institute, Rana Pratap Marg , Lucknow , India.
authors.forename: Chandra Shekhar
authors.initials: CS
authors.lastname: Nautiyal
mesh_terms: D000069977:Bacillus amyloliquefaciens / Q000187:drug effects / Q000502:physiology*; D044822:Biodiversity; D001681:Biological Assay; D002244:Carbon / Q000494:pharmacology; D029863:Cicer / Q000201:enzymology / Q000254:growth & development / Q000382:microbiology* / Q000502:physiology*; D055864:Droughts; D018515:Plant Leaves / Q000187:drug effects / Q000382:microbiology / Q000502:physiology; D025341:Principal Component Analysis; D016958:Pseudomonas putida / Q000187:drug effects / Q000502:physiology*; D058441:Rhizosphere
chemical_list: D002244:Carbon
keywords: PGPR; biofertilizers; consortia; rhizosphere; synergism