title: Haemosporidian infections in the Tengmalm's Owl (Aegolius funereus) and potential insect vectors of their transmission.
abstract: Sedentary bird species are suitable model hosts for identifying potential vectors of avian blood parasites. We studied haemosporidian infections in the Tengmalm's Owl (Aegolius funereus) in the Ore Mountains of the Czech Republic using molecular detection methods. Sex of owl nestlings was scored using molecular sexing based on fragment analysis of PCR-amplified CHD1 introns. Observed infection prevalences in nestlings and adult owls were 51 and 86 %, respectively. Five parasite lineages were detected. Most of the infections comprised the Leucocytozoon AEFUN02 and STOCC06 lineages that probably refer to distinct Leucocytozoon species. Other lineages were detected only sporadically. Mixed infections were found in 49 % of samples. The main factor affecting the probability of infection was host age. No effect of individual sex on infection probability was evidenced. The youngest infected nestling was 12 days old. High parasite prevalence in the Tengmalm's Owl nestlings suggests that insect vectors must enter nest boxes to transmit parasites before fledging. Hence, we placed sticky insect traps into modified nest boxes, collected potential insect vectors, and examined them for the presence of haemosporidian parasites using molecular detection. We trapped 201 insects which were determined as biting midges from the Culicoides genus and two black fly species, Simulium (Nevermannia) vernum and Simulium (Eusimulium) angustipes. Six haemosporidian lineages were detected in the potential insect vectors, among which the Leucocytozoon lineage BT2 was common to the Tengmalm's Owl and the trapped insects. However, we have not detected the most frequently encountered Tengmalm's Owl Leucocytozoon lineages AEFUN02 and STOCC06 in insects.
authors.affiliation: Department of Zoology, Faculty of Science, Charles University in Prague, Viničná 7, 128 00, Prague 2, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Synek
authors.affiliation: Department of Ecology, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Kamýcká 129, 165 21, Prague 6 - Suchdol, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Popelková
authors.affiliation: Department of Ecology and Evolution, University of Lausanne, UNIL Sorge, Biophore Building, 1015, Lausanne, Switzerland.
authors.forename: Darina
authors.initials: D
authors.lastname: Koubínová
authors.affiliation: Department of Ecology, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Kamýcká 129, 165 21, Prague 6 - Suchdol, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Šťastný
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 21, Prague 6 - Suchdol, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Langrová
authors.affiliation: Department of Parasitology, Faculty of Science, Charles University in Prague, Viničná 7, 128 00, Prague 2, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Votýpka
authors.affiliation: Department of Zoology, Faculty of Science, Charles University in Prague, Viničná 7, 128 00, Prague 2, Czech Republic. muncling@natur.cuni.cz.
authors.forename: Pavel
authors.initials: P
authors.lastname: Munclinger
mesh_terms: D000367:Age Factors; D000818:Animals; D001715:Bird Diseases / Q000453:epidemiology / Q000469:parasitology* / Q000635:transmission; D002519:Ceratopogonidae / Q000469:parasitology; D018153:Czech Republic / Q000453:epidemiology; D005260:Female; D016787:Haemosporida / Q000235:genetics / Q000302:isolation & purification*; D007303:Insect Vectors / Q000469:parasitology*; D008297:Male; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011529:Protozoan Infections, Animal / Q000453:epidemiology / Q000469:parasitology* / Q000635:transmission; D012737:Sex Factors; D012843:Simuliidae / Q000469:parasitology; D020305:Strigiformes / Q000469:parasitology*
keywords: Avian malaria; Blood parasites; Molecular sexing of owls; Strigiformes; Transmission; Vectors; Wildlife diseases