title: Effects of Nanotexture on Electrical Profiling of Single Tumor Cell and Detection of Cancer from Blood in Microfluidic Channels.
abstract: Microfluidic channels have been implemented to detect cancer cells from blood using electrical measurement of each single cell from the sample. Every cell provided characteristic current profile based on its mechano-physical properties. Cancer cells not only showed higher translocation time and peak amplitude compared to blood cells, their pulse shape was also distinctively different. Prevalent microfluidic channels are plain but we created nanotexture on the channel walls using micro reactive ion etching (micro-RIE). The translocation behaviors of the metastatic renal cancer cells through plain and nanotextured PDMS microchannels showed clear differences. Nanotexture enhanced the cell-surface interactions and more than 50% tumor cells exhibited slower translocation through nanotextured channels compared to plain devices. On the other hand, most of the blood cells had very similar characteristics in both channels. Only 7.63% blood cells had slower translocation in nanotextured microchannels. The tumor cell detection efficiency from whole blood increased by 14% in nanotextured microchannels compared to plain channels. This interesting effect of nanotexture on translocation behavior of tumor cells is important for the early detection of cancer.
authors.affiliation: Nano-Bio Lab, University of Texas at Arlington, Arlington, TX 76019, USA.|Department of Electrical Engineering, University of Texas at Arlington, Arlington, TX 76011, USA.|Nanotechnology Research Center, University of Texas at Arlington, Arlington, TX 76019, USA.
authors.forename: Muhymin
authors.initials: M
authors.lastname: Islam
authors.affiliation: Nano-Bio Lab, University of Texas at Arlington, Arlington, TX 76019, USA.|Nanotechnology Research Center, University of Texas at Arlington, Arlington, TX 76019, USA.|Department of Interdisciplinary Studies and Department of Biology, University of Texas at Arlington, Arlington, TX 76011, USA.
authors.forename: Mohammad Motasim
authors.initials: MM
authors.lastname: Bellah
authors.affiliation: Nano-Bio Lab, University of Texas at Arlington, Arlington, TX 76019, USA.|Nanotechnology Research Center, University of Texas at Arlington, Arlington, TX 76019, USA.|Department of Interdisciplinary Studies and Department of Biology, University of Texas at Arlington, Arlington, TX 76011, USA.
authors.forename: Adeel
authors.initials: A
authors.lastname: Sajid
authors.affiliation: Nano-Bio Lab, University of Texas at Arlington, Arlington, TX 76019, USA.|Department of Electrical Engineering, University of Texas at Arlington, Arlington, TX 76011, USA.|Nanotechnology Research Center, University of Texas at Arlington, Arlington, TX 76019, USA.
authors.forename: Mohammad Raziul
authors.initials: MR
authors.lastname: Hasan
authors.affiliation: Nanotechnology Research Center, University of Texas at Arlington, Arlington, TX 76019, USA.|Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76010, USA.|Department of Urology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, 75390, USA.
authors.forename: Young-tae
authors.initials: YT
authors.lastname: Kim
authors.affiliation: Nano-Bio Lab, University of Texas at Arlington, Arlington, TX 76019, USA.|Department of Electrical Engineering, University of Texas at Arlington, Arlington, TX 76011, USA.|Nanotechnology Research Center, University of Texas at Arlington, Arlington, TX 76019, USA.|Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76010, USA.|Department of Urology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, 75390, USA.
authors.forename: Samir M
authors.initials: SM
authors.lastname: Iqbal
mesh_terms: D000818:Animals; D001773:Blood Cells; D001932:Brain Neoplasms / Q000556:secondary; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis; D009360:Neoplastic Cells, Circulating; D051381:Rats; D059010:Single-Cell Analysis / Q000379:methods*