title: Decoding protein networks during virus entry by quantitative proteomics.
abstract: Virus entry into host cells relies on interactions between viral and host structures including lipids, carbohydrates and proteins. Particularly, protein-protein interactions between viral surface proteins and host proteins as well as secondary host protein-protein interactions play a pivotal role in coordinating virus binding and uptake. These interactions are dynamic and frequently involve multiprotein complexes. In the past decade mass spectrometry based proteomics methods have reached sensitivities and high throughput compatibilities of genomics methods and now allow the reliable quantitation of proteins in complex samples from limited material. As proteomics provides essential information on the biologically active entity namely the protein, including its posttranslational modifications and its interactions with other proteins, it is an indispensable method in the virologist's toolbox. Here we review protein interactions during virus entry and compare classical biochemical methods to study entry with novel technically advanced quantitative proteomics techniques. We highlight the value of quantitative proteomics in mapping functional virus entry networks, discuss the benefits and limitations and illustrate how the methodology will help resolve unsettled questions in virus entry research in the future.
authors.affiliation: Insitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, A joint Venture Between the Medical School Hannover and the Helmholtz Centre for Infection Research, 30652 Hannover, Germany. Electronic address: gisa.gerold@twincore.de.
authors.forename: Gisa
authors.initials: G
authors.lastname: Gerold
authors.affiliation: Insitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, A joint Venture Between the Medical School Hannover and the Helmholtz Centre for Infection Research, 30652 Hannover, Germany.
authors.forename: Janina
authors.initials: J
authors.lastname: Bruening
authors.affiliation: Insitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, A joint Venture Between the Medical School Hannover and the Helmholtz Centre for Infection Research, 30652 Hannover, Germany; German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, Germany. Electronic address: thomas.pietschmann@twincore.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Pietschmann
mesh_terms: D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006801:Humans; D016023:Integrins / Q000235:genetics / Q000378:metabolism; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism; D025941:Protein Interaction Mapping; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D011991:Receptors, Virus / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D014777:Virus Diseases / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D053586:Virus Internalization; D014780:Viruses / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D016023:Integrins; D037181:Lectins, C-Type; D046912:Multiprotein Complexes; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; D014764:Viral Proteins; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
keywords: Protein interaction networks; Protein–protein interactions; Quantitative proteomics; Virus entry