title: Detection and Validation of QTL Affecting Bacterial Cold Water Disease Resistance in Rainbow Trout Using Restriction-Site Associated DNA Sequencing.
abstract: Bacterial cold water disease (BCWD) causes significant economic loss in salmonid aquaculture. Using microsatellite markers in a genome scan, we previously detected significant and suggestive QTL affecting phenotypic variation in survival following challenge with Flavobacterium psychrophilum, the causative agent of BCWD in rainbow trout. In this study, we performed selective genotyping of SNPs from restriction-site associated DNA (RAD) sequence data from two pedigreed families (2009070 and 2009196) to validate the major QTL from the previous work and to detect new QTL. The use of RAD SNPs in the genome scans increased the number of mapped markers from ~300 to ~5,000 per family. The significant QTL detected in the microsatellites scan on chromosome Omy8 in family 2009070 was validated explaining up to 58% of the phenotypic variance in that family, and in addition, a second QTL was also detected on Omy8. Two novel QTL on Omy11 and 14 were also detected, and the previously suggestive QTL on Omy1, 7 and 25 were also validated in family 2009070. In family 2009196, the microsatellite significant QTL on Omy6 and 12 were validated and a new QTL on Omy8 was detected, but none of the previously detected suggestive QTL were validated. The two Omy8 QTL from family 2009070 and the Omy12 QTL from family 2009196 were found to be co-localized with handling and confinement stress response QTL that our group has previously identified in a separate pedigreed family. With the currently available data we cannot determine if the co-localized QTL are the result of genes with pleiotropic effects or a mere physical proximity on the same chromosome segment. The genetic markers linked to BCWD resistance QTL were used to query the scaffolds of the rainbow trout reference genome assembly and the QTL-positive scaffold sequences were found to include 100 positional candidate genes. Several of the candidate genes located on or near the two Omy8 QTL detected in family 2009070 suggest potential linkages between stress response and the regulation of immune response in rainbow trout.
authors.affiliation: National Center for Cool and Cold Water Aquaculture, ARS-USDA, 11861 Leetown Rd., Kearneysville, West Virginia, 25430, United States of America.
authors.forename: Yniv
authors.initials: Y
authors.lastname: Palti
authors.affiliation: National Center for Cool and Cold Water Aquaculture, ARS-USDA, 11861 Leetown Rd., Kearneysville, West Virginia, 25430, United States of America.
authors.forename: Roger L
authors.initials: RL
authors.lastname: Vallejo
authors.affiliation: National Center for Cool and Cold Water Aquaculture, ARS-USDA, 11861 Leetown Rd., Kearneysville, West Virginia, 25430, United States of America.
authors.forename: Guangtu
authors.initials: G
authors.lastname: Gao
authors.affiliation: National Center for Cool and Cold Water Aquaculture, ARS-USDA, 11861 Leetown Rd., Kearneysville, West Virginia, 25430, United States of America.
authors.forename: Sixin
authors.initials: S
authors.lastname: Liu
authors.affiliation: University of Illinois at Urbana-Champaign, Roy J Carver Biotechnology Center, 1201 W. Gregory Dr., Urbana, Illinois, 61801, United States of America.
authors.forename: Alvaro G
authors.initials: AG
authors.lastname: Hernandez
authors.affiliation: National Center for Cool and Cold Water Aquaculture, ARS-USDA, 11861 Leetown Rd., Kearneysville, West Virginia, 25430, United States of America.
authors.forename: Caird E
authors.initials: CE
authors.lastname: Rexroad
authors.affiliation: National Center for Cool and Cold Water Aquaculture, ARS-USDA, 11861 Leetown Rd., Kearneysville, West Virginia, 25430, United States of America.
authors.forename: Gregory D
authors.initials: GD
authors.lastname: Wiens
mesh_terms: D000818:Animals; D060467:Disease Resistance / Q000235:genetics*; D005393:Fish Diseases / Q000235:genetics / Q000382:microbiology*; D045826:Flavobacteriaceae Infections / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D005417:Flavobacterium / Q000472:pathogenicity; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics*; D016678:Genome; D055106:Genome-Wide Association Study; D005838:Genotype; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000254:growth & development; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D005819:Genetic Markers