title: A century later: rediscovery, culturing and phylogenetic analysis of Diploöspora rosea, a rare onygenalean hyphomycete.
abstract: Nearly 100 years after its first discovery, Diploöspora rosea was detected on biologically damaged parchment paper in Rome, Italy and isolated from house dust collected in Micronesia. The isolation of this culture permitted morphological study of colony characters, conidium and conidiophore development, and phylogenetic investigations using sequences of nuc 18S rDNA, internal transcribed spacers, and 28S rDNA. The results indicate that D. rosea is an onygenalean fungus, of uncertain taxonomic position, basal or sister to the Gymnoascaceae. Based on observations of the parchments using SEM-Energy Dispersive Spectroscopy, we speculate that the fungus occurs in archival and domestic environments subject to periodic wetting. Its ability to grow on all low water activity media used in the study, including malt extract agar amended with 60% sucrose, confirms its xerophilic nature.
authors.affiliation: Biodiversity (Mycology and Microbiology), Agriculture and Agri-Food Canada, Ottawa, ON, K1A 0C6, Canada. jtanney@lakeheadu.ca.
authors.forename: Joey B
authors.initials: JB
authors.lastname: Tanney
authors.affiliation: Biodiversity (Mycology and Microbiology), Agriculture and Agri-Food Canada, Ottawa, ON, K1A 0C6, Canada.
authors.forename: Hai D T
authors.initials: HD
authors.lastname: Nguyen
authors.affiliation: Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria (CREA), Rome, Italy.|Department of Life Sciences, Natural History Museum, London, UK.
authors.forename: Flavia
authors.initials: F
authors.lastname: Pinzari
authors.affiliation: Biodiversity (Mycology and Microbiology), Agriculture and Agri-Food Canada, Ottawa, ON, K1A 0C6, Canada.
authors.forename: Keith A
authors.initials: KA
authors.lastname: Seifert
mesh_terms: D058893:DNA Barcoding, Taxonomic; D021903:DNA, Ribosomal Spacer; D003904:Mitosporic Fungi / Q000145:classification* / Q000166:cytology / Q000235:genetics* / Q000648:ultrastructure; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D012339:RNA, Ribosomal, 28S / Q000235:genetics*
chemical_list: D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S
keywords: 28S nrDNA; Gymnoascaceae; House dust; ITS; Onygenales; Phylogeny; Taxonomy