title: Rapid, Single-Cell Electrochemical Detection of Mycobacterium tuberculosis Using Colloidal Gold Nanoparticles.
abstract: Tuberculosis (TB) remains a global health threat, with over a third of the world population suffering from the disease, and 1.5 million deaths due to the disease in 2013 alone. Despite significant advances in TB detection strategies in recent years, a bigger push toward detecting TB in the shortest and easiest way possible at the point-of-care (POC) is still in demand. To this end, we have designed a simple yet rapid and sensitive bioassay that detects Mtb DNA electrochemically using colloidal gold nanoparticles. This assay couples rapid isothermal amplification of target DNA that is specific to Mtb with gold nanoparticle electrochemistry on disposable screen printed carbon electrodes. The assay is capable of detecting a positive differential pulse voltammetry (DPV) response from as low as 1 CFU of Mtb bacilli DNA input material, having shown its exquisite sensitivity over a conventional gel based readout. The translation of our assay onto a portable potentiostat was also demonstrated, with promising results. We believe that our assay has significant potential for translation into broader bioassay applications or development as a POC diagnostic tool.
authors.affiliation: Centre for Personalized NanoMedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland , Brisbane, QLD 4072, Australia.|School of Chemistry and Molecular Biosciences, The University of Queensland , Brisbane, QLD 4072, Australia.
authors.forename: Benjamin Y C
authors.initials: BY
authors.lastname: Ng
authors.affiliation: Centre for Personalized NanoMedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland , Brisbane, QLD 4072, Australia.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.affiliation: School of Chemistry and Molecular Biosciences, The University of Queensland , Brisbane, QLD 4072, Australia.
authors.forename: Nicholas P
authors.initials: NP
authors.lastname: West
authors.affiliation: Centre for Personalized NanoMedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland , Brisbane, QLD 4072, Australia.
authors.forename: Eugene J H
authors.initials: EJ
authors.lastname: Wee
authors.affiliation: Centre for Personalized NanoMedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland , Brisbane, QLD 4072, Australia.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Centre for Personalized NanoMedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland , Brisbane, QLD 4072, Australia.|School of Chemistry and Molecular Biosciences, The University of Queensland , Brisbane, QLD 4072, Australia.
authors.forename: Matt
authors.initials: M
authors.lastname: Trau
mesh_terms: D002244:Carbon; D004269:DNA, Bacterial / Q000032:analysis; D055664:Electrochemical Techniques; D004566:Electrodes; D017979:Gold Colloid / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D019095:Point-of-Care Systems; D059010:Single-Cell Analysis
chemical_list: D004269:DNA, Bacterial; D017979:Gold Colloid; D002244:Carbon