title: DNA-hydrolysing activity of IgG antibodies from the sera of patients with schizophrenia.
abstract: It is believed that damage to the membranes of brain cells of schizophrenia (SCZ) patients induces the formation of autoantigens and autoantibodies. Nevertheless, the importance of immunological changes leading to the loss of tolerance to self-antigens in the genesis of SCZ has not been established. The MALDI mass spectra of the IgG light chains of 20 healthy donors were relatively homogeneous and characterized by one peak with only one maximum. In contrast to the healthy donors, the MALDI mass spectra of IgG light chains corresponding to 20 SCZ patients demonstrated, similarly to 20 autoimmune systemic lupus erythematosus (SLE) patients, two maxima of a comparable intensity. In addition, the MALDI spectra of the IgG light chains of five SLE and four SCZ patients contained a small additional brightly pronounced peak with remarkably lower molecular mass compared with the main one. DNase autoantibodies (abzymes) can be found in the blood of patients with several autoimmune diseases, while the blood of healthy donors or patients with diseases without a significant disturbance of the immune status does not contain DNase abzymes. Here, we present the first analysis of anti-DNA antibodies and DNase abzymes in the sera of SCZ patients. Several strict criteria have been applied to show that the DNase activity is an intrinsic property of IgGs from the sera of SCZ patients. The sera of approximately 30% of SCZ patients displayed a higher content of antibodies (compared with 37% of SLE) interacting with single- and double-stranded DNA compared with healthy donors. Antibodies with DNase activity were revealed in 80% of the patients. These data indicate that some SCZ patients may show signs of typical autoimmune processes to a certain extent.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences, 8 Lavrentiev Avenue, Novosibirsk 630090, Russia Novosibirsk State University, 2 Pirogova Street, Novosibirsk 630090, Russia.
authors.forename: Evgeny A
authors.initials: EA
authors.lastname: Ermakov
authors.affiliation: Mental Health Research Institute, Russian Academy of Medical Sciences, 4 Aleutskaya Avenue, Tomsk 634014, Russia.
authors.forename: Ludmila P
authors.initials: LP
authors.lastname: Smirnova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences, 8 Lavrentiev Avenue, Novosibirsk 630090, Russia.
authors.forename: Taisiya A
authors.initials: TA
authors.lastname: Parkhomenko
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, Vladivostok 690022, Russia.
authors.forename: Pavel S
authors.initials: PS
authors.lastname: Dmitrenok
authors.affiliation: Mental Health Research Institute, Russian Academy of Medical Sciences, 4 Aleutskaya Avenue, Tomsk 634014, Russia.
authors.forename: Nina M
authors.initials: NM
authors.lastname: Krotenko
authors.affiliation: Mental Health Research Institute, Russian Academy of Medical Sciences, 4 Aleutskaya Avenue, Tomsk 634014, Russia.
authors.forename: Nikolai S
authors.initials: NS
authors.lastname: Fattakhov
authors.affiliation: Mental Health Research Institute, Russian Academy of Medical Sciences, 4 Aleutskaya Avenue, Tomsk 634014, Russia.
authors.forename: Nikolay A
authors.initials: NA
authors.lastname: Bokhan
authors.affiliation: Mental Health Research Institute, Russian Academy of Medical Sciences, 4 Aleutskaya Avenue, Tomsk 634014, Russia.
authors.forename: Arkadiy V
authors.initials: AV
authors.lastname: Semke
authors.affiliation: Mental Health Research Institute, Russian Academy of Medical Sciences, 4 Aleutskaya Avenue, Tomsk 634014, Russia.
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Ivanova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences, 8 Lavrentiev Avenue, Novosibirsk 630090, Russia Novosibirsk State University, 2 Pirogova Street, Novosibirsk 630090, Russia.
authors.forename: Valentina N
authors.initials: VN
authors.lastname: Buneva
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences, 8 Lavrentiev Avenue, Novosibirsk 630090, Russia Novosibirsk State University, 2 Pirogova Street, Novosibirsk 630090, Russia nevinsky@niboch.nsc.ru.
authors.forename: Georgy A
authors.initials: GA
authors.lastname: Nevinsky
mesh_terms: D000328:Adult; D017151:Antibodies, Catalytic / Q000097:blood / Q000378:metabolism; D001323:Autoantibodies / Q000097:blood; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D007147:Immunoglobulin Light Chains / Q000737:chemistry; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D012559:Schizophrenia / Q000097:blood* / Q000378:metabolism / Q000473:pathology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D017151:Antibodies, Catalytic; D001323:Autoantibodies; D004277:DNA, Single-Stranded; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D004247:DNA; D003851:Deoxyribonucleases
keywords: DNA hydrolysis; abzymes; autoimmune reactions; schizophrenia