title: NF-κB-dependent signals control BOB.1/OBF.1 and Oct2 transcriptional activity in B cells.
abstract: The transcriptional co-activator BOB.1/OBF.1 is crucial for Octamer-driven transcription in B cells. BOB.1/OBF.1-deficiency leads to tremendous defects in B-cell development and function. Therefore, in the past research focused on the identification of BOB.1/OBF.1 target genes. However, the regulation of BOB.1/OBF.1 expression itself is poorly understood. Here we show that in B cells NF-κB as well as to some extent NFAT proteins are involved in the activation of basal as well as inducible BOB.1/OBF.1 expression by direct binding to the BOB.1/OBF.1 promoter. Moreover, the analysis of different inducers of NF-κB, like several TLR ligands, TNF-α, BAFF, or LTα1β2, revealed that both the canonical and noncanonical NF-κB pathways are involved in the induction of BOB.1/OBF.1 gene. The identification of so far unknown inducers that regulate BOB.1/OBF.1 expression in B cells provides novel insights in the potential function of BOB.1/OBF.1 during different aspects of B-cell development and function.
authors.affiliation: Institute of Physiological Chemistry, Ulm University, Ulm, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Kilzheimer
authors.affiliation: Institute of Physiological Chemistry, Ulm University, Ulm, Germany.
authors.forename: Jasmin
authors.initials: J
authors.lastname: Quandt
authors.affiliation: Department of Otorhinolaryngology, Ulm University, Ulm, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Langhans
authors.affiliation: Institute of Physiological Chemistry, Ulm University, Ulm, Germany.|Department of Otorhinolaryngology, Ulm University, Ulm, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Weihrich
authors.affiliation: Institute of Physiological Chemistry, Ulm University, Ulm, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Wirth
authors.affiliation: Institute of Physiological Chemistry, Ulm University, Ulm, Germany.|Department of Otorhinolaryngology, Ulm University, Ulm, Germany.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Brunner
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000502:physiology*; D050778:NFATC Transcription Factors / Q000378:metabolism; D027701:Organic Cation Transport Proteins / Q000235:genetics*; D000075102:Organic Cation Transporter 2; D011401:Promoter Regions, Genetic; D015398:Signal Transduction / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D016328:NF-kappa B; D050778:NFATC Transcription Factors; D027701:Organic Cation Transport Proteins; D000075102:Organic Cation Transporter 2; C490424:Pou2af1 protein, mouse; C491012:Slc22a2 protein, mouse; D015534:Trans-Activators
keywords: B cells • BOB.1/OBF.1 (Pou2af1) • Oct2 (Pou2f2) • promoter analyses • transcriptional regulation