title: Evaluation of Kinase Activity Profiling Using Chemical Proteomics.
abstract: Protein kinases are important mediators of intracellular signaling and are reversibly activated by phosphorylation. Immobilized kinase inhibitors can be used to enrich these often low-abundance proteins, to identify targets of kinase inhibitors, or to probe their selectivity. It has been suggested that the binding of kinases to affinity beads reflects a kinase's activation status, a concept that is under considerable debate. To assess the merits of the idea, we performed a series of experiments including quantitative phosphoproteomics and purification of kinases by single or mixed affinity matrices from signaling activated or resting cancer cells. The data show that mixed affinity beads largely bind kinases independent of their activation status, and experiments using individual immobilized kinase inhibitors show mixed results in terms of preference for binding the active or inactive conformation. Taken together, activity- or conformation-dependent binding to such affinity resins depends (i) on the kinase, (ii) on the affinity probe, and (iii) on the activation status of the lysate or cell. As a result, great caution should be exercised when inferring kinase activity from such binding data. The results also suggest that assaying kinase activity using binding data is restricted to a limited number of well-chosen cases.
authors.affiliation: Chair of Proteomics and Bioanalytics, Technische Universität München , Emil-Erlenemeyer-Forum 5, 85354 Freising, Germany.|Center for Protein Science Munich (CIPSM) , 85354 Freising, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Ruprecht
authors.affiliation: Chair of Proteomics and Bioanalytics, Technische Universität München , Emil-Erlenemeyer-Forum 5, 85354 Freising, Germany.|German Cancer Consortium (DKTK) , 69120 Heidelberg, Germany.|German Cancer Research Center (DKFZ) , 69121 Heidelberg, Germany.
authors.forename: Jana
authors.initials: J
authors.lastname: Zecha
authors.affiliation: Chair of Proteomics and Bioanalytics, Technische Universität München , Emil-Erlenemeyer-Forum 5, 85354 Freising, Germany.|German Cancer Consortium (DKTK) , 69120 Heidelberg, Germany.|German Cancer Research Center (DKFZ) , 69121 Heidelberg, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Heinzlmeir
authors.affiliation: Chair of Proteomics and Bioanalytics, Technische Universität München , Emil-Erlenemeyer-Forum 5, 85354 Freising, Germany.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Médard
authors.affiliation: Chair of Proteomics and Bioanalytics, Technische Universität München , Emil-Erlenemeyer-Forum 5, 85354 Freising, Germany.|Center for Protein Science Munich (CIPSM) , 85354 Freising, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Lemeer
authors.affiliation: Chair of Proteomics and Bioanalytics, Technische Universität München , Emil-Erlenemeyer-Forum 5, 85354 Freising, Germany.|Center for Protein Science Munich (CIPSM) , 85354 Freising, Germany.|German Cancer Consortium (DKTK) , 69120 Heidelberg, Germany.|German Cancer Research Center (DKFZ) , 69121 Heidelberg, Germany.|Bavarian Biomolecular Mass Spectrometry Center, Technische Universität München , 85354 Freising, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Kuster
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D013058:Mass Spectrometry; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D014638:Vanadates / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; C076715:pervanadate; D014638:Vanadates; D011494:Protein Kinases