title: Partial in vitro analysis of toxic and antigenic activities of eleven Peruvian pitviper snake venoms.
abstract: This work used eleven Peruvian snake venoms (Bothrops andianus, Bothrops atrox, Bothrops barnetti, Bothrops castelnaudi, Bothriopsis chloromelas, Bothrocophias microphthalmus, Bothrops neuwiedi, Bothriopsis oligolepis, Bothriopsis peruviana, Bothrops pictus and Bothriopsis taeniata) to perform in vitro experimentation and determine its main characteristics. Hyaluronidase (HYAL), phospholipase A2 (PLA2), snake venom metalloproteinase (SVMP), snake venom serine protease (SVSP) and L-amino acid oxidase (LAAO) activities; toxicity by cell viability assays using MGSO3, VERO and HeLa cell lineages; and crossed immunoreactivity with Peruvian (PAV) and Brazilian (BAV) antibothropic polyvalent antivenoms, through ELISA and Western Blotting assays, were determined. Results show that the activities tested in this study were not similar amongst the venoms and each species present their own peculiarities, highlighting the diversity within Bothrops complex. All venoms were capable of reducing cell viability of all tested lineages. It was also demonstrated the crossed recognition of all tested venoms by both antivenoms.
authors.affiliation: Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Guerra-Duarte
authors.affiliation: Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Lopes-Peixoto
authors.affiliation: Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: B R
authors.initials: BR
authors.lastname: Fonseca-de-Souza
authors.affiliation: Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: S
authors.initials: S
authors.lastname: Stransky
authors.affiliation: Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Oliveira
authors.affiliation: Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: F S
authors.initials: FS
authors.lastname: Schneider
authors.affiliation: Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Lopes-de-Souza
authors.affiliation: Instituto Nacional de Salud, Lima, Peru.
authors.forename: C
authors.initials: C
authors.lastname: Bonilla
authors.affiliation: Instituto Nacional de Salud, Lima, Peru.
authors.forename: W
authors.initials: W
authors.lastname: Silva
authors.affiliation: Instituto Nacional de Salud, Lima, Peru.
authors.forename: B
authors.initials: B
authors.lastname: Tintaya
authors.affiliation: Universidad Nacional Mayor de San Marcos, Lima, Peru.
authors.forename: A
authors.initials: A
authors.lastname: Yarleque
authors.affiliation: Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil. Electronic address: olortegi@icb.ufmg.br.
authors.forename: C
authors.initials: C
authors.lastname: Chávez-Olórtegui
mesh_terms: D000818:Animals; D000997:Antivenins / Q000494:pharmacology*; D015153:Blotting, Western; D017837:Bothrops; D001938:Brazil; D002460:Cell Line; D002522:Chlorocebus aethiops; D003435:Crotalid Venoms / Q000201:enzymology / Q000276:immunology / Q000633:toxicity*; D004797:Enzyme-Linked Immunosorbent Assay; D006367:HeLa Cells; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D050830:L-Amino Acid Oxidase / Q000378:metabolism; D045726:Metalloproteases / Q000378:metabolism; D010568:Peru; D054467:Phospholipases A2 / Q000378:metabolism; D057057:Serine Proteases / Q000378:metabolism; D014709:Vero Cells
chemical_list: D000997:Antivenins; D003435:Crotalid Venoms; D050830:L-Amino Acid Oxidase; D054467:Phospholipases A2; D006821:Hyaluronoglucosaminidase; D045726:Metalloproteases; D057057:Serine Proteases
keywords: In vitro assays; Peruvian snakes; Venom characterization