title: High mobility group box 1 (HMGB1) is upregulated by the Epstein-Barr virus infection and promotes the proliferation of human nasopharyngeal carcinoma cells.
abstract: CONCLUSIONS
  The current study confirmed the significant high mobility group box 1 (HMGB1) was promoted in human nasopharyngeal carcinoma (NPC) tissues by Epstein-Barr virus (EBV) infection, in association with the malignant status of NPC, and promoted the proliferation NPC cells RAGE-dependently.
  
  
  OBJECTIVE
  The present study was to examine the association of HMGB1 over-expression in human NPC with the EBV-positivity and to determine the regulatory role of HMGB1 on the proliferation of NPC cells in vitro.
  
  
  METHODS
  Real-time PCR and Western blotting were utilized to examine the HMGB1 expression. EBV infection in CNE-2 cells was performed to investigate the HMGB1 promotion by EBV infection. RNA interference technology was utilized for the RAGE knockout.
  
  
  RESULTS
  It was demonstrated that HMGB1 was significantly higher in both mRNA and protein levels in the EBV-positive NPC tissues, in marked association with the malignant status of NPC, and with the LMP1 DNA level in EBV-positive NPC samples. In addition, the MTT assay, growth curve, and the colony forming assay confirmed the promotion by HMGB1 to the proliferation of CNE-2 cells, depending on RAGE.
authors.affiliation: a Department of Otolaryngology, Head & Neck Surgery , China-Japan Union Hospital, Jilin University , Changchun , PR China and.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Zhu
authors.affiliation: b Department of Otolaryngology, Head & Neck Surgery , First Hospital of Jilin University , Changchun , PR China.
authors.forename: Le
authors.initials: L
authors.lastname: Sun
authors.affiliation: b Department of Otolaryngology, Head & Neck Surgery , First Hospital of Jilin University , Changchun , PR China.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000328:Adult; D002277:Carcinoma; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D005260:Female; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D024243:HMGB1 Protein; D012333:RNA, Messenger
keywords: Epstein-Barr virus (EBV); high mobility group box 1 (HMGB1); nasopharyngeal carcinoma (NPC); proliferation; receptor for advanced glycation end products (RAGE)