title: An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake.
abstract: The fern Pteris vittata is an arsenic hyperaccumulator. The genes involved in arsenite (As(III)) transport are not yet clear. Here, we describe the isolation and characterization of a new P. vittata aquaporin gene, PvTIP4;1, which may mediate As(III) uptake. PvTIP4;1 was identified from yeast functional complement cDNA library of P. vittata. Arsenic toxicity and accumulating activities of PvTIP4;1 were analyzed in Saccharomyces cerevisiae and Arabidopsis. Subcellular localization of PvTIP4;1-GFP fusion protein in P. vittata protoplast and callus was conducted. The tissue expression of PvTIP4;1 was investigated by quantitative real-time PCR. Site-directed mutagenesis of the PvTIP4;1 aromatic/arginine (Ar/R) domain was studied. Heterologous expression in yeast demonstrates that PvTIP4;1 was able to facilitate As(III) diffusion. Transgenic Arabidopsis showed that PvTIP4;1 increases arsenic accumulation and induces arsenic sensitivity. Images and FM4-64 staining suggest that PvTIP4;1 localizes to the plasma membrane in P. vittata cells. A tissue location study shows that PvTIP4;1 transcripts are mainly expressed in roots. Site-directed mutation in yeast further proved that the cysteine at the LE1 position of PvTIP4;1 Ar/R domain is a functional site. PvTIP4;1 is a new represented tonoplast intrinsic protein (TIP) aquaporin from P. vittata and the function and location results imply that PvTIP4;1 may be involved in As(III) uptake.
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors.forename: Zhenyan
authors.initials: Z
authors.lastname: He
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Huili
authors.initials: H
authors.lastname: Yan
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Yanshan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Shen
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors.forename: Wenxiu
authors.initials: W
authors.lastname: Xu
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China.
authors.forename: Yong-Guan
authors.initials: YG
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Ma
mesh_terms: D020346:Aquaporins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D001151:Arsenic / Q000633:toxicity; D018053:Arsenites / Q000378:metabolism / Q000493:pharmacokinetics*; D001692:Biological Transport; D003545:Cysteine; D018506:Gene Expression Regulation, Plant; D008565:Membrane Proteins / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D032495:Pteris / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: D020346:Aquaporins; D018053:Arsenites; C093263:FPS1 protein, S cerevisiae; D008565:Membrane Proteins; D010940:Plant Proteins; D029701:Saccharomyces cerevisiae Proteins; D003545:Cysteine; C015001:arsenite; D001151:Arsenic
keywords: Pteris vittata; PvTIP4;1; aquaporin; aromatic/arginine (Ar/R); arsenic (As); arsenite (As(III)); uptake