title: Characterization and expression analysis of laboratory of genetics and physiology 2 gene in sea perch, Lateolabrax japonicus.
abstract: LGP2 (laboratory of genetics and physiology 2) as a key component of the retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs), plays a predominant role in modulating RLRs-mediated cellular antiviral signaling during viral infection. In the present study, we cloned the LGP2 gene from the sea perch (Lateolabrax japonicus) (LjLGP2), an economically important farmed fish. The complete cDNA sequence of LjLGP2 was 2790 nt and encoded a polypeptide of 682 amino acids which contains four main structural domains: one DEAD/DEAH box helicase domain, one conserved restriction domain of bacterial type III restriction enzyme, one helicase superfamily c-terminal domain and one C-terminal domain of RIG-I, similar to most vertebrate LGP2. Subcellular localization analysis showed that LjLGP2 spanned the entire cytosol. The LjLGP2 mRNA was widespread expressed in the tested 10 tissues of healthy fish and significantly up-regulated post NNV infection. Furthermore, time course analysis showed that LjLGP2 transcripts signiﬁcantly increased in the spleen, kidney and liver tissues after NNV infection. LjLGP2 mRNA expression was rapidly and significantly up-regulated in LJB cells after poly I:C stimulation and NNV infection. The present results suggest that LjLGP2 may be involved in recognization of NNV and play a role in antiviral innate immune against NNV in sea perch.
authors.affiliation: School of Marine Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-sen University, Guangzhou, Guangdong, China. Electronic address: jiapeng19890219@126.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Jia
authors.affiliation: School of Marine Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-sen University, Guangzhou, Guangdong, China. Electronic address: zhangj358@mail2.sysu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Marine Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-sen University, Guangzhou, Guangdong, China. Electronic address: 369277368@qq.com.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Jin
authors.affiliation: School of Marine Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-sen University, Guangzhou, Guangdong, China. Electronic address: zen9.lin@gmail.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Zeng
authors.affiliation: School of Marine Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-sen University, Guangzhou, Guangdong, China. Electronic address: jiakuntong@126.com.
authors.forename: Kuntong
authors.initials: K
authors.lastname: Jia
authors.affiliation: School of Marine Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-sen University, Guangzhou, Guangdong, China. Electronic address: yimsh@mail.sysu.edu.cn.
authors.forename: Meisheng
authors.initials: M
authors.lastname: Yi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D030381:Nodaviridae / Q000502:physiology; D009928:Organ Specificity; D010473:Perciformes; D010802:Phylogeny; D011070:Poly I-C / Q000494:pharmacology; D012327:RNA Virus Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D011070:Poly I-C
keywords: Laboratory of genetics and physiology 2; Lateolabrax japonicus; Nervous necrosis virus; RIG-I-like receptors