title: Combination of RNA sequencing and metabolite data to elucidate improved toxic compound tolerance and butanol fermentation of Clostridium acetobutylicum from wheat straw hydrolysate by supplying sodium sulfide.
abstract: Sodium sulfide (SS) was added to the non-detoxified wheat straw hydrolysate for ABE fermentation by Clostridium acetobutylicum CICC8012. Biochemical measurements demonstrated that supplementation with SS promoted earlier and enhanced conversion of acid to ABE and led to a 27.48% improvement in sugar consumption, a 20.48% improvement in the sugar-based ABE yield, a 47.63% improvement in the butanol titer, and a 53.50% improvement in the ABE concentration. The response of C. acetobutylicum CICC8012 at the mRNA level was examined by a transcriptional analysis performed with RNA sequencing. The expression of genes involved in the membrane transport of carbohydrates, glycolysis, and ABE formation increased following SS-supplemented fermentation, whereas the expression of genes encoding enzymes involved in acid formation decreased, which indicates that supplemental SS affected the central fermentative pathway, down-regulated the metabolic flux toward the acid formation branches, and up-regulated the metabolic flux toward the ABE formation branches.
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Mengjun
authors.initials: M
authors.lastname: Huang
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jiaolong
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Gao
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Renqiang
authors.initials: R
authors.lastname: Li
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China.
authors.forename: Kaize
authors.initials: K
authors.lastname: He
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China. Electronic address: zhaohai@cib.ac.cn.
authors.forename: Hai
authors.initials: H
authors.lastname: Zhao
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D001483:Base Sequence; D002241:Carbohydrates; D046969:Clostridium acetobutylicum / Q000378:metabolism*; D004361:Drug Tolerance; D005285:Fermentation; D006601:Hexoses / Q000378:metabolism; D006868:Hydrolysis; D017423:Sequence Analysis, RNA; D013440:Sulfides; D014908:Triticum / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D006601:Hexoses; D013440:Sulfides; D020001:1-Butanol; C033479:sodium sulfide
keywords: Butanol; Clostridium acetobutylicum; RNA sequencing; Sodium sulfide; Transcriptional analysis