title: NMR resonance assignments for the class II GTP binding RNA aptamer in complex with GTP.
abstract: The structures of RNA-aptamer-ligand complexes solved in the last two decades were instrumental in realizing the amazing potential of RNA for forming complex tertiary structures and for molecular recognition of small molecules. For GTP as ligand the sequences and secondary structures for multiple families of aptamers were reported which differ widely in their structural complexity, ligand affinity and ligand functional groups involved in RNA-binding. However, for only one of these families the structure of the GTP-RNA complex was solved. In order to gain further insights into the variability of ligand recognition modes we are currently determining the structure of another GTP-aptamer--the so-called class II aptamer--bound to GTP using NMR-spectroscopy in solution. As a prerequisite for a full structure determination, we report here (1)H, (13)C, (15)N and partial (31)P-NMR resonance assignments for the class II GTP-aptamer bound to GTP.
authors.affiliation: Institute for Molecular Biosciences, Johann-Wolfgang-Goethe-University, Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.|Center for Biomolecular Magnetic Resonance (BMRZ), Johann-Wolfgang-Goethe-University, Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.
authors.forename: Antje C
authors.initials: AC
authors.lastname: Wolter
authors.affiliation: Institute for Molecular Biosciences, Johann-Wolfgang-Goethe-University, Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.|Center for Biomolecular Magnetic Resonance (BMRZ), Johann-Wolfgang-Goethe-University, Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Duchardt-Ferner
authors.affiliation: Institute for Molecular Biosciences, Johann-Wolfgang-Goethe-University, Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.|Center for Biomolecular Magnetic Resonance (BMRZ), Johann-Wolfgang-Goethe-University, Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.
authors.forename: Amir H
authors.initials: AH
authors.lastname: Nasiri
authors.affiliation: Institute for Molecular Biosciences, Johann-Wolfgang-Goethe-University, Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.|Center for Biomolecular Magnetic Resonance (BMRZ), Johann-Wolfgang-Goethe-University, Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Hantke
authors.affiliation: Institute of Organic Chemistry, Centre for Molecular Biosciences (CMBI), University of Innsbruck, Innrain 80/82, 6020, Innsbruck, Austria.
authors.forename: Christoph H
authors.initials: CH
authors.lastname: Wunderlich
authors.affiliation: Institute of Organic Chemistry, Centre for Molecular Biosciences (CMBI), University of Innsbruck, Innrain 80/82, 6020, Innsbruck, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Kreutz
authors.affiliation: Institute for Molecular Biosciences, Johann-Wolfgang-Goethe-University, Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany. woehnert@bio.uni-frankfurt.de.|Center for Biomolecular Magnetic Resonance (BMRZ), Johann-Wolfgang-Goethe-University, Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany. woehnert@bio.uni-frankfurt.de.
authors.forename: Jens
authors.initials: J
authors.lastname: Wöhnert
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism*; D001483:Base Sequence; D006160:Guanosine Triphosphate / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation
chemical_list: D052157:Aptamers, Nucleotide; D006160:Guanosine Triphosphate
keywords: Aptamer; GTP; NMR-assignment; RNA; Selective labeling; Triple resonance experiments