title: Expression of CCM2 and CCM3 during mouse gonadogenesis.
abstract: OBJECTIVE
  Three cerebral cavernous malformation (CCM) proteins, CCM1, CCM2, and CCM3, regulate cell-cell adhesion, cell shape and polarity, and most likely cell adhesion to extracellular matrix. Recently, CCM2 and CCM3 are known to be expressed in control and varicocele-induced rat testes, but little is known about these proteins during gonadogenesis. This led us to study the CCM proteins during the mouse gonadogenesis.
  
  
  METHODS
  Neonatal (PND 0), postnatal, and adult mice testes and ovaries were obtained from mice. CCM2 and CCM3 expression were analyzed during mouse testicular and ovarian development by immunohistochemistry and quantitative real-time PCR.
  
  
  RESULTS
  The results showed that in both sexes, Ccm2 and Ccm3 mRNA and protein were first detectable after gonadogenesis when the gonads were well differentiated and remained present until the adult stage. In the testis, CCM2 and CCM3 expression were restricted to the nuclei of Sertoli cells, suggesting a conserved role in testicular differentiation. In the ovary, the CCM2 and CCM3 proteins were localized in the cytoplasm of oocytes, suggesting an unexpected role during oogenesis. Quantitative real-time PCR (qRT-PCR) results showed that expression of Ccm2 and Ccm3 genes could play a role in the regulation of mouse gonadogenesis translational activation upon testicular and ovarian development.
  
  
  CONCLUSIONS
  The localization of CCM2 and CCM3 proteins show their different functions for CCM2 and CCM3 which may have important roles in testicular and ovarian differentiation. In conclusion, CCM2 and CCM3 may be involved in establishing the differential expression pattern in developing mouse testis and ovary.
authors.affiliation: Department of Histology and Embryology, İstanbul Medipol University School of Medicine, İstanbul, Turkey.
authors.forename: Aylin
authors.initials: A
authors.lastname: Yaba
authors.affiliation: Department of Histology and Embryology, Akdeniz University School of Medicine, 07070 Campus, Antalya, Turkey.
authors.forename: N Ece Gungor
authors.initials: NE
authors.lastname: Ordueri
authors.affiliation: Department of Histology and Embryology, Akdeniz University School of Medicine, 07070 Campus, Antalya, Turkey.
authors.forename: Gamze
authors.initials: G
authors.lastname: Tanriover
authors.affiliation: Department of Histology and Embryology, Akdeniz University School of Medicine, 07070 Campus, Antalya, Turkey.
authors.forename: Pinar
authors.initials: P
authors.lastname: Sahin
authors.affiliation: Department of Histology and Embryology, Akdeniz University School of Medicine, 07070 Campus, Antalya, Turkey.
authors.forename: Necdet
authors.initials: N
authors.lastname: Demir
authors.affiliation: Department of Histology and Embryology, Akdeniz University School of Medicine, 07070 Campus, Antalya, Turkey. cilerozenci@akdeniz.edu.tr.
authors.forename: Ciler
authors.initials: C
authors.lastname: Celik-Ozenci
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D051017:Apoptosis Regulatory Proteins; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D000075927:KRIT1 Protein; D008297:Male; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D009865:Oocytes / Q000502:physiology; D010053:Ovary / Q000166:cytology / Q000254:growth & development* / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D012708:Sertoli Cells / Q000502:physiology; D013737:Testis / Q000166:cytology / Q000254:growth & development* / Q000502:physiology
chemical_list: D051017:Apoptosis Regulatory Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000075927:KRIT1 Protein; C491535:Krit1 protein, mouse; D008840:Microfilament Proteins; D008869:Microtubule-Associated Proteins; C559318:PDCD10 protein, mouse; D011518:Proto-Oncogene Proteins; C480498:osmosensing scaffold for MEKK3 protein, mouse
keywords: CCM2; CCM3; Gonadogenesis; Mouse