title: [Succinic acid production from sucrose and sugarcane molasses by metabolically engineered Escherichia coli].
abstract: Sugarcane molasses containing large amounts of sucrose is an economical substrate for succinic acid production. However, Escherichia coli AFP111 cannot metabolize sucrose although it is a promising candidate for succinic acid production. To achieve sucrose utilizing ability, we cloned and expressed cscBKA genes encoding sucrose permease, fructokinase and invertase of non-PTS sucrose-utilization system from E. coli W in E. coli AFP111 to generate a recombinant strain AFP111/pMD19T-cscBKA. After 72 h of anaerobic fermentation of the recombinant in serum bottles, 20 g/L sucrose was consumed and 12 g/L succinic acid was produced. During dual-phase fermentation comprised of initial aerobic growth phase followed by anaerobic fermentation phase, the concentration of succinic acid from sucrose and sugarcane molasses was 34 g/L and 30 g/L, respectively, at 30 h of anaerobic phase in a 3 L fermentor. The results show that the introduction of non-PTS sucrose-utilization system has sucrose-metabolizing capability for cell growth and succinic acid production, and can use cheap sugarcane molasses to produce succinic acid.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Ma
authors.forename: Mingke
authors.initials: M
authors.lastname: Wu
authors.forename: Yaliang
authors.initials: Y
authors.lastname: Ji
authors.forename: Wufang
authors.initials: W
authors.lastname: Chen
authors.forename: Xinyi
authors.initials: X
authors.lastname: Ren
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
mesh_terms: D019149:Bioreactors; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics; D005285:Fermentation; D026901:Membrane Transport Proteins / Q000235:genetics; D060847:Metabolic Engineering; D008965:Molasses; D031786:Saccharum / Q000737:chemistry*; D019802:Succinic Acid / Q000737:chemistry*; D013395:Sucrose / Q000737:chemistry*
chemical_list: D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; C056379:cscB protein, E coli; D013395:Sucrose; D019802:Succinic Acid