title: miRNAs regulated overexpression of ryanodine receptor is involved in chlorantraniliprole resistance in Plutella xylostella (L.).
abstract: The amino acid mutations in ryanodine receptor (RyR) and elevated activity of detoxification enzymes have been associated with the diamide insecticide resistance in the diamondback moth, Plutella xylostella (L.). The up-regulation of P. xylostella RyR mRNA (PxRyR) expression has also been reported in field populations of different graphical origin. However, whether the up-regulation of PxRyR is involved in diamide resistance remains unknown. In this paper, 2.28- to 4.14-fold higher expression of PxRyR was detected in five field collected resistant populations, compared to that in a susceptible population. The expression of PxRyR was up-regulated 5.0- and 7.2-fold, respectively, after P. xylostella was treated with LC50 and LC75 of chlorantraniliprole for 12 h. Suppression of PxRyR using RNA interference restored the toxicity of chlorantraniliprole against the fourth instar larvae from the resistant population. More importantly, the expression of PxRyR is regulated by two miRNAs, miR-7a and miR-8519. These findings provide an empirical evidence of the involvement of miRNAs in the regulation of insecticide resistance, and shed light on the novel targets for the sustainable management of this devastating insect pest.
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Xiuxia
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P. R. China.|College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Entomology, University of Kentucky, Lexington, KY 40546-0091, USA.
authors.forename: Xuguo
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Xiwu
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liang
mesh_terms: D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D055786:Gene Knockout Techniques; D007305:Insecticide Resistance / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D009036:Moths / Q000187:drug effects* / Q000235:genetics*; D034622:RNA Interference; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics*; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
chemical_list: D035683:MicroRNAs; D019837:Ryanodine Receptor Calcium Release Channel; D062367:ortho-Aminobenzoates; C517733:chlorantranilipole