title: Early Diagnosis of Cutaneous Mucormycosis Due to Lichtheimia corymbifera After a Traffic Accident.
abstract: A case report of cutaneous mucormycosis and obstacles to early diagnosis is presented. A 38-year-old male was involved in a car accident that led to amputation of both lower limbs. Subsequently, he developed fungal wound infection of the left lower limb stump. The infection was detected very early, although the diagnosis was difficult because only a small area was affected and histopathological examination was initially negative. The infection was proven by microscopy, culture and histopathology. The isolate was identified by sequencing of the rDNA ITS region gene (internal transcribed spacer region of ribosomal DNA) as Lichtheimia corymbifera. Liposomal amphotericin B and surgery were successful in management of the disease.
authors.affiliation: Department of Anesthesiology, Resuscitation and Intensive Care Medicine, First Faculty of Medicine, Charles University in Prague and the Military University Hospital Prague, U Vojenske Nemocnice 1200, 169 02, Prague 6, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Tyll
authors.affiliation: Laboratory of Medical Mycology, Department of Parasitology, Mycology and Mycobacteriology Prague, Public Health Institute in Usti nad Labem, Sokolovska 60, 186 00, Prague 8, Czech Republic. pavlina.lyskova@zuusti.cz.|Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Hnevotinska 3, 77515, Olomouc, Czech Republic. pavlina.lyskova@zuusti.cz.
authors.forename: Pavlina
authors.initials: P
authors.lastname: Lyskova
authors.affiliation: Department of Botany, Faculty of Science, Charles University in Prague, Benatska 2, 128 01, Praha 2, Czech Republic.|Laboratory of Fungal Genetics and Metabolism, Institute of Microbiology of the AS CR, Videnska 1083, 142 20, Praha 4, Czech Republic.|First Faculty of Medicine, Charles University in Prague, Katerinska 32, 121 08, Prague 2, Czech Republic.
authors.forename: Vit
authors.initials: V
authors.lastname: Hubka
authors.affiliation: Department of Anesthesiology, Resuscitation and Intensive Care Medicine, First Faculty of Medicine, Charles University in Prague and the Military University Hospital Prague, U Vojenske Nemocnice 1200, 169 02, Prague 6, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Muller
authors.affiliation: Department of Orthopaedics, First Faculty of Medicine, Charles University in Prague and the Military University Hospital Prague, U Vojenske Nemocnice 1200, 169 02, Prague 6, Czech Republic.
authors.forename: Lubomir
authors.initials: L
authors.lastname: Zelenka
authors.affiliation: Department of Clinical Microbiology, Military University Hospital Prague, U Vojenske Nemocnice 1200, 169 02, Prague 6, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Curdova
authors.affiliation: Department of Pathology, Military University Hospital Prague, U Vojenske Nemocnice 1200, 169 02, Prague 6, Czech Republic.
authors.forename: Inna
authors.initials: I
authors.lastname: Tuckova
authors.affiliation: Department of Botany, Faculty of Science, Charles University in Prague, Benatska 2, 128 01, Praha 2, Czech Republic.|Laboratory of Fungal Genetics and Metabolism, Institute of Microbiology of the AS CR, Videnska 1083, 142 20, Praha 4, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Kolarik
authors.affiliation: Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Hnevotinska 3, 77515, Olomouc, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Hamal
mesh_terms: D000063:Accidents, Traffic; D000328:Adult; D000666:Amphotericin B / Q000008:administration & dosage; D000935:Antifungal Agents / Q000008:administration & dosage; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D003646:Debridement; D042241:Early Diagnosis; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008853:Microscopy; D009090:Mucorales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009091:Mucormycosis / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D017422:Sequence Analysis, DNA; D016896:Treatment Outcome; D014946:Wound Infection / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D014947:Wounds and Injuries / Q000150:complications*
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; C068538:liposomal amphotericin B; D000666:Amphotericin B
keywords: Amphotericin B; Cutaneous mucormycosis; Lichtheimia corymbifera; Mycological diagnosis