title: Use of Centrifugal Filter Devices to Concentrate Dengue Virus in Mosquito per os Infection Experiments.
abstract: Dengue virus (DENV) is an arbovirus transmitted to humans by the bite of infected Aedes mosquitoes. Experimental per os infection of mosquitoes with DENV is usually a preliminary step in virus/vector studies but it requires being able to prepare artificial blood-meals with high virus titers. We report here the convenient use of centrifugal filter devices to quickly concentrate DENV particles in cell-culture supernatants. The median viral titer in concentrated-supernatants was 8.50 log10 TCID50/mL. By using these DENV concentrated-supernatants to prepare infectious blood-meals in Aedes aegypti per os infection experiments, we obtained a mean mosquito-infection rate of 94%. We also evaluated the use of centrifugal filter devices to recover DENV particles from non-infectious blood-meals presented to infected mosquitoes through a feeding membrane to collect their saliva.
authors.affiliation: Institut Louis Malardé, Papeete, French Polynesia.
authors.forename: Vaea
authors.initials: V
authors.lastname: Richard
authors.affiliation: Institut Louis Malardé, Papeete, French Polynesia.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Viallon
authors.affiliation: Institut Louis Malardé, Papeete, French Polynesia.
authors.forename: Van-Mai
authors.initials: VM
authors.lastname: Cao-Lormeau
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D001769:Blood / Q000821:virology; D002498:Centrifugation / Q000295:instrumentation*; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000302:isolation & purification* / Q000502:physiology; D005374:Filtration / Q000295:instrumentation*; D007303:Insect Vectors / Q000821:virology; D012463:Saliva / Q000821:virology