title: Cellular compatibility of magnetic nanocomposites.
abstract: OBJECTIVE
  To study the cellular compatibility of the magnetic nanocomposite (n-HA/PLLA/Fe2O3) and to discuss the biological behaviors of cells including surface adhesion, proliferation, and expression. The present work provides an experimental basis for medical application.
  
  
  METHODS
  Rat osteoblasts (OB) were co-cultured with the magnetic nanocomposite. Cell proliferation, cell adhesion, and the expression of type I collagen (Col-I) and osteocalcin (OCN) gene were characterized by the cell counting kit-8 (CCK-8) method, scanning electronic microscopy (SEM), and reverse transcription polymerase chain reaction (RT-PCR), respectively.
  
  
  RESULTS
  CCK-8 detection showed that there was no difference in cell proliferation on the magnetic nanocomposite between the experimental group and control group (P > 0.05). SEM indicated that a large amount of cells adhered to the surface and in the pores of the magnetic nanocomposite. As the co-culture time increased, the cells adhering to the magnetic nanocomposite showed an obvious increase. RT-PCR detection showed that as the co-culture time increased, the expression of the Col-I gene was enhanced (P 0.05). There was no obvious difference in the expression of the OCN gene (P > 0.05).
  
  
  CONCLUSIONS
  The magnetic nanocomposite is suitable for cell adhesion, growth, and differentiation with a high cellular compatibility.
authors.affiliation: a School of Medicine, Sun Yat-sen University , Guangzhou 510182, Guangdong Province, China.|d Shenzhen Tissue Engineering Laboratory, Shenzhen Second People's Hospital , Shenzhen , P. R. China.
authors.forename: Jie-Lin
authors.initials: JL
authors.lastname: Chen
authors.affiliation: b Department of Sports Medicine , The A liated Clinical College Shenzhen Second People's Hospital, Anhui Medical University , Hefei , P. R. China.|c Department of Sports Medicine , The First A liated Hospital of Shenzhen University (Shenzhen Second People's Hospital) , Shenzhen , P. R. China.|d Shenzhen Tissue Engineering Laboratory, Shenzhen Second People's Hospital , Shenzhen , P. R. China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhu
authors.affiliation: b Department of Sports Medicine , The A liated Clinical College Shenzhen Second People's Hospital, Anhui Medical University , Hefei , P. R. China.|c Department of Sports Medicine , The First A liated Hospital of Shenzhen University (Shenzhen Second People's Hospital) , Shenzhen , P. R. China.|d Shenzhen Tissue Engineering Laboratory, Shenzhen Second People's Hospital , Shenzhen , P. R. China.
authors.forename: Daiqi
authors.initials: D
authors.lastname: Guo
authors.affiliation: b Department of Sports Medicine , The A liated Clinical College Shenzhen Second People's Hospital, Anhui Medical University , Hefei , P. R. China.
authors.forename: Weili
authors.initials: W
authors.lastname: Xu
authors.affiliation: c Department of Sports Medicine , The First A liated Hospital of Shenzhen University (Shenzhen Second People's Hospital) , Shenzhen , P. R. China.|d Shenzhen Tissue Engineering Laboratory, Shenzhen Second People's Hospital , Shenzhen , P. R. China.
authors.forename: Yunfang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: c Department of Sports Medicine , The First A liated Hospital of Shenzhen University (Shenzhen Second People's Hospital) , Shenzhen , P. R. China.
authors.forename: Jianyi
authors.initials: J
authors.lastname: Xiong
authors.affiliation: c Department of Sports Medicine , The First A liated Hospital of Shenzhen University (Shenzhen Second People's Hospital) , Shenzhen , P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: d Shenzhen Tissue Engineering Laboratory, Shenzhen Second People's Hospital , Shenzhen , P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Duan
authors.affiliation: e Shenzhen Digital Orthopaedic Technology Engineering Laboratory, Shenzhen Second People's Hospital , Shenzhen , P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: You
authors.affiliation: b Department of Sports Medicine , The A liated Clinical College Shenzhen Second People's Hospital, Anhui Medical University , Hefei , P. R. China.|c Department of Sports Medicine , The First A liated Hospital of Shenzhen University (Shenzhen Second People's Hospital) , Shenzhen , P. R. China.|d Shenzhen Tissue Engineering Laboratory, Shenzhen Second People's Hospital , Shenzhen , P. R. China.|e Shenzhen Digital Orthopaedic Technology Engineering Laboratory, Shenzhen Second People's Hospital , Shenzhen , P. R. China.
authors.forename: Daping
authors.initials: D
authors.lastname: Wang
authors.affiliation: f Biomechanics and Medical Information Institute, Beijing University of Technology , Beijing , P. R. China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats
chemical_list: D058185:Magnetite Nanoparticles
keywords: cellular compatibility; composite; magnetic; nanomaterial; osteoblast