title: Hippocampal synaptotagmin-4 is correlated with impaired spatial learning and memory in SAMP8 mice.
abstract: The mechanism underlying age-related cognitive impairment remains unclear. To determine whether synaptotagmin (Syt)-1 and Syt-4 are involved in age-related cognitive impairment, we used a radial six-arm water maze (RAWM) to evaluate spatial learning and memory deficits in the senescence accelerated prone mouse 8. The Syt-1 and Syt-4 levels of different subregions of the dorsal hippocampus (DH) were detected through immunohistochemistry. The RAWM results revealed that 13- and 9-month-old mice exhibited longer latencies and more errors in both the learning and memory phases than 5-month-old mice. Similar results were observed in the comparison of 13-month-old mice to 9-month-old mice. Compared with the 9- and/or 5-month-old mice, the 13-month-old mice exhibited higher Syt-1 and Syt-4 levels in the majority of the DH subregions with the exception of Syt-1 in the dentate gyrus-hilus and Syt-4 in the dentate gyrus-hilus and cornu ammonis 1 pyramidal cell layer. With the exception of Syt-1 in the 9-month-old mice, the Syt-1 and Syt-4 levels in several DH subregions overall and in each group were significantly correlated with the performances on the RAWM. Therefore, the altered Syt-1 and Syt-4 levels in the different DH layers may have been involved in the impairments in spatial learning and memory during normal aging.
authors.affiliation: The First Affiliated Hospital of Anhui Medical University, Hefei, PR China.
authors.forename: Qi-Gang
authors.initials: QG
authors.lastname: Yang
authors.affiliation: The Affiliated Chaohu Hospital of Anhui Medical University, Hefei, PR China. Electronic address: doctorcgh@163.com.
authors.forename: Gui-Hai
authors.initials: GH
authors.lastname: Chen
authors.affiliation: The First Affiliated Hospital of Anhui Medical University, Hefei, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Anhui Medical University, Hefei, PR China.
authors.forename: Long-Hai
authors.initials: LH
authors.lastname: Wang
mesh_terms: D000818:Animals; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D008568:Memory; D051379:Mice; D065853:Spatial Learning; D050863:Synaptotagmin I / Q000378:metabolism; D050857:Synaptotagmins / Q000378:metabolism*
chemical_list: D050863:Synaptotagmin I; D050857:Synaptotagmins
keywords: Aging; Hippocampus; Learning and memory; Mice; Synaptotagmin