title: Peripheral administration of palmitoylated prolactin-releasing peptide induces Fos expression in hypothalamic neurons involved in energy homeostasis in NMRI male mice.
abstract: Energy homeostasis is the result of a balance between energy intake and expenditure, and the hypothalamus plays a key role in the regulation of these processes. The hypothalamic prolactin-releasing peptide (PrRP) is involved in food intake regulation and energy homeostasis, although only its lipidized analogs exert central anorexigenic effects after peripheral administration. The aim of the present study was to delineate the extent of the Fos expression as a marker of neuronal activation within the hypothalamic structures involved in food intake regulation after peripherally administered palmitoylated PrRP31 (palm-PrRP31) and to determine whether the anorexigenic effect of peripherally administered palm-PrRP31 influence the activity of hypocretin (HCRT) and oxytocin (OXY) neurons, i.e., the neuropeptides crucially involved in the regulation of energy homeostasis. The data confirmed an anorexigenic effect of palm-PrRP31 treatment (5mg/kg, s.c.) in mice. In the palm-PrRP31-treated animals, a significant increase in Fos expression was observed in the hypothalamic paraventricular (PVN), dorsomedial (DMN), and arcuate (Arc) nuclei and in the neurons of the nucleus of the solitary tract (NTS). Moreover, significant Fos expression was observed in the lateral hypothalamic area (LHA) HCRT neurons and PVN OXY neurons after palm-PrRP31 administration. The present findings may indicate that palm-PrRP31 may be involved in energy homeostasis via the activation of several hypothalamic structures. Fos activation of the hypothalamic OXY and HCRT neurons in the PVN and LHA emphasizes the importance of the areas mentioned in the central action of palm-PrRP31.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, AS CR, Prague, Czech Republic; Laboratory of Functional Neuromorphology, Institute of Experimental Endocrinology, SAS, Bratislava, Slovak Republic; Department of Human and Clinical Pharmacology, University of Veterinary Medicine, Košice, Slovak Republic.
authors.forename: Zdenko
authors.initials: Z
authors.lastname: Pirnik
authors.affiliation: Institute of Organic Chemistry and Biochemistry, AS CR, Prague, Czech Republic.
authors.forename: Blanka
authors.initials: B
authors.lastname: Železná
authors.affiliation: Laboratory of Functional Neuromorphology, Institute of Experimental Endocrinology, SAS, Bratislava, Slovak Republic.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kiss
authors.affiliation: Institute of Organic Chemistry and Biochemistry, AS CR, Prague, Czech Republic. Electronic address: maletin@uochb.cas.cz.
authors.forename: Lenka
authors.initials: L
authors.lastname: Maletínská
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004435:Eating / Q000187:drug effects; D004734:Energy Metabolism / Q000187:drug effects*; D007031:Hypothalamus / Q000166:cytology* / Q000187:drug effects; D008297:Male; D051379:Mice; D009474:Neurons / Q000187:drug effects*; D016761:Oncogene Proteins v-fos / Q000378:metabolism*; D000068797:Orexins / Q000378:metabolism; D010121:Oxytocin / Q000378:metabolism; D056690:Prolactin-Releasing Hormone / Q000494:pharmacology*
chemical_list: C000596233:Hcrt protein, mouse; D016761:Oncogene Proteins v-fos; D000068797:Orexins; D056690:Prolactin-Releasing Hormone; D010121:Oxytocin
keywords: Fos; Hypocretin; Hypothalamus; Mice; Oxytocin; Prolactin-releasing peptide