title: Comparative plasma proteomic studies of pulmonary TiO2 nanoparticle exposure in rats using liquid chromatography tandem mass spectrometry.
abstract: Mounting evidence suggests that pulmonary exposure to nanoparticles (NPs) has a toxic effect on biological systems. A number of studies have shown that exposure to NPs result in systemic inflammatory response, oxidative stress, and leukocyte adhesion. However, significant knowledge gaps exist for understanding the key molecular mechanisms responsible for altered microvasculature function. Utilizing comprehensive LC-MS/MS and comparative proteomic analysis strategies, important proteins related to TiO2 NP exposure in rat plasma have been identified. Molecular pathway analysis of these proteins revealed 13 canonical pathways as being significant (p ≤ 0.05), but none were found to be significantly up or down-regulated (z>|2|). This work lays the foundation for future research that will monitor relative changes in protein abundance in plasma and tissue as a function of post-exposure time and TiO2 NP dosage to further elucidate mechanisms of pathway activation as well as to decipher other affected pathways.
authors.affiliation: C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506, United States.
authors.forename: Megan M
authors.initials: MM
authors.lastname: Maurer
authors.affiliation: C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506, United States.
authors.forename: Gregory C
authors.initials: GC
authors.lastname: Donohoe
authors.affiliation: C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506, United States.
authors.forename: Hossein
authors.initials: H
authors.lastname: Maleki
authors.affiliation: Department of Physiology and Pharmacology, West Virginia University, Morgantown, WV 26506, United States.
authors.forename: Jinghai
authors.initials: J
authors.lastname: Yi
authors.affiliation: Department of Physiology and Pharmacology, West Virginia University, Morgantown, WV 26506, United States.
authors.forename: Carroll
authors.initials: C
authors.lastname: McBride
authors.affiliation: Department of Physiology and Pharmacology, West Virginia University, Morgantown, WV 26506, United States.
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Nurkiewicz
authors.affiliation: C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506, United States. Electronic address: stephen.valentine@mail.wvu.edu.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Valentine
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D001798:Blood Proteins / Q000378:metabolism*; D002853:Chromatography, Liquid; D019570:Inhalation Exposure; D008099:Liver / Q000473:pathology; D008168:Lung / Q000187:drug effects*; D008297:Male; D053768:Metal Nanoparticles / Q000009:adverse effects* / Q000737:chemistry; D025341:Principal Component Analysis; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry; D014025:Titanium / Q000009:adverse effects* / Q000737:chemistry
chemical_list: D001798:Blood Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; C009495:titanium dioxide; D014025:Titanium
keywords: Biopathway analysis; Proteomics; TiO(2) nanoparticles