title: MicroRNA-1 effectively induces differentiation of myocardial cells from mouse bone marrow mesenchymal stem cells.
abstract: In this research, bone marrow mesenchymal stem cells (BMSCs) were isolated from mouse, and induced differentiation into myocardial cells in vitro after overexpression of miR-1a. The results showed that the BMSCs could induce differentiation into myocardial cells under the special condition medium, but when the miR-1a was over-expressed in BMSCs, the differentiation efficiency and induction time of myocardial cells from BMSCs could be promoted. This reason was demonstrated that Delta-like 1 (Dll-1) was a transcriptional repressor of myocardium gene expression during myocardium differentiation, miR-1a reduced Dll-1 levels, leading to the accumulation of myocardium gene mRNA and a dramatic increase in myocardium gene protein.
authors.affiliation: a Department of Cardiology , Renmin Hospital of Wuhan University , Wuhan , Hubei , China and.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Zhao
authors.affiliation: a Department of Cardiology , Renmin Hospital of Wuhan University , Wuhan , Hubei , China and.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: b Department of Geriatrics , Xuan Wu Hospital, Capital Medical University , Beijing , China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Ma
authors.affiliation: b Department of Geriatrics , Xuan Wu Hospital, Capital Medical University , Beijing , China.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Dong
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002135:Calcium-Binding Proteins; D002454:Cell Differentiation; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; C512925:Dlk1 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; D035683:MicroRNAs; C517731:Mirn1 microRNA, mouse
keywords: BMSCs; differentiation; microRNAs; myocardial cells