title: Conversion of dried Aspergillus candidus mycelia grown on waste whey to biodiesel by in situ acid transesterification.
abstract: This study reports optimization of the transesterification reaction step on dried biomass of an oleaginous fungus Aspergillus candidus grown on agro-dairy waste, whey. Acid catalyzed transesterification was performed and variables affecting esterification, viz., catalyst methanol and chloroform concentrations, temperature, time, and biomass were investigated. Statistical optimization of the transesterification reaction using Plackett-Burman Design showed biomass to be the predominant factor with a 12.5-fold increase in total FAME from 25.6 to 320mg. Studies indicate that the transesterification efficiency in terms of conversion is favored by employing lower biomass loadings. A. candidus exhibited FAME profiles containing desirable saturated (30.2%), monounsaturated (31.5%) and polyunsaturated methyl esters (38.3%). The predicted and experimentally determined biodiesel properties (density, kinematic viscosity, iodine value, cetane number, TAN, water content, total and free glycerol) were in accordance with international (ASTM D6751, EN 14214) and national (IS 15607) standards.
authors.affiliation: Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune 411007, India.
authors.forename: Hardik
authors.initials: H
authors.lastname: Kakkad
authors.affiliation: Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune 411007, India.
authors.forename: Mahesh
authors.initials: M
authors.lastname: Khot
authors.affiliation: Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune 411007, India.
authors.forename: Smita
authors.initials: S
authors.lastname: Zinjarde
authors.affiliation: Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune 411007, India. Electronic address: ameeta@unipune.ac.in.
authors.forename: Ameeta
authors.initials: A
authors.lastname: RaviKumar
authors.affiliation: Chemical Engineering and Process Development Division, CSIR-National Chemical Laboratory (CSIR-NCL), Pune 411008, India.
authors.forename: V
authors.initials: V
authors.lastname: Ravi Kumar
authors.affiliation: Chemical Engineering and Process Development Division, CSIR-National Chemical Laboratory (CSIR-NCL), Pune 411008, India.
authors.forename: B D
authors.initials: BD
authors.lastname: Kulkarni
mesh_terms: D001230:Aspergillus / Q000737:chemistry*; D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002384:Catalysis; D002725:Chloroform / Q000737:chemistry; D004951:Esterification; D004952:Esters / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D007220:Industrial Waste; D000432:Methanol / Q000737:chemistry; D025282:Mycelium / Q000737:chemistry; D013696:Temperature; D000067796:Whey
chemical_list: D056804:Biofuels; D004952:Esters; D005227:Fatty Acids; D007220:Industrial Waste; D002725:Chloroform; D000432:Methanol
keywords: Aspergillus candidus; Biodiesel; In situ acid transesterification; Plackett–Burman Design; Whey