title: p53 mediated apoptosis in osteosarcoma MG-63 cells by inhibition of FANCD2 gene expression.
abstract: OBJECTIVE
  The aim of this study was to investigate the association between osteosarcoma (OS) and Fanconi anemia (FA) related pathways and the molecular mechanisms.
  
  
  METHODS
  siRNA for Fanconi anemia complementation group D2 (FANCD2) was constructed and transfected into the osteosarcoma cell line MG-63 cells. Expression of TP53INP1, p53, p21, caspase-9, and caspase-3 mRNA in MG-63 cells were examined by real-time fluorescence quantitative PCR, and the protein levels were also determined by western blot.
  
  
  RESULTS
  After silence of the FANCD2 gene in MG-63 cells, cell proliferation was inhibited, cell cycle was arrested and cell apoptosis was induced. The apoptosis was mediated by the p53 signaling pathway. After FANCD2 expression was inhibited, TP53INP1 gene expression was up-regulated, phosphorylation of p53 was promoted and the p21 protein was activated, leading to cell cycle arrested in G1, finally resulted in caspase-dependent cell apoptosis.
  
  
  CONCLUSIONS
  Inhibition of FANCD2 gene expression can induce apoptosis of osteosarcoma cells, which indicated that FANCD2 played an important role in the development of osteosarcoma and it might be a potential target for treatment of osteosarcoma.
authors.affiliation: Department of Orthopedics, The Second Hospital of Jilin University Changchun 130000, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xia
authors.affiliation: Department of Orthopedics, China Japan Union Hospital of Jilin University Changchun 130000, China.
authors.forename: Yifu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Orthopedics, The Second Hospital of Jilin University Changchun 130000, China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Department of Orthopedics, The Second Hospital of Jilin University Changchun 130000, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Hou
authors.affiliation: Department of Orthopedics, The Second Hospital of Jilin University Changchun 130000, China.
authors.forename: Mingyang
authors.initials: M
authors.lastname: Kang
authors.affiliation: Department of Orthopedics, The Second Hospital of Jilin University Changchun 130000, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Yang
keywords: Fanconi anemia complementation group D2; RNA interference; cell apoptosis; osteosarcoma; p53