title: BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties.
abstract: This study describes the biochemical and functional characterization of a new metalloproteinase named BbMP-1, isolated from Bothrops brazili venom. BbMP-1 was homogeneous on SDS-PAGE, presented molecular mass of 22,933Da and pI 6.4. The primary structure was partially elucidated with high identity with others metalloproteinases from Viperidae venoms. The enzymatic activity on azocasein was evaluated in different experimental conditions (pH, temperature). A significant reduction in enzyme activity after exposure to chelators of divalent cations (EDTA), reducing agents (DTT), pH less than 5.0 or temperatures higher than 45 °C was observed. BbMP-1 showed activity on fibrinogen degrading Aα chain quickly and to a lesser extent the Bβ chain. Also demostrated to be weakly hemorrhagic, presenting however, significant myotoxic and edematogenic activity. The in vitro activity of BbMP-1 against Plasmodium falciparum showed an IC50 of 3.2 ± 2.0 μg/mL. This study may help to understand the pathophysiological effects induced by this group of toxin and their participation in the symptoms observed in cases of snake envenomation. Moreover, this result is representative for this group of proteins and shows the biotechnological potential of BbMP-1 by the demonstration of its antiplasmodial activity.
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, Fiocruz Rondônia e Programa de Pós Graduação em Biologia Experimental, PGBIOEXP, Núcleo de Saúde, NUSAU, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil.
authors.forename: Anderson M
authors.initials: AM
authors.lastname: Kayano
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, Fiocruz Rondônia e Programa de Pós Graduação em Biologia Experimental, PGBIOEXP, Núcleo de Saúde, NUSAU, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Simões-Silva
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, Fiocruz Rondônia e Programa de Pós Graduação em Biologia Experimental, PGBIOEXP, Núcleo de Saúde, NUSAU, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil.
authors.forename: Patrícia S M
authors.initials: PS
authors.lastname: Medeiros
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André, SP, Brazil; Departamento de Farmácia, Faculdade de Ciências Farmacêuticas, USP, SP, Brazil.
authors.forename: Vinícius G
authors.initials: VG
authors.lastname: Maltarollo
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André, SP, Brazil; Escola de Artes, Ciências e Humanidades, USP, São Paulo, SP, Brazil.
authors.forename: Kathia M
authors.initials: KM
authors.lastname: Honorio
authors.affiliation: Proteomic Platform, Barcelona Science Park, 08028 Barcelona, Spain.
authors.forename: Eliandre
authors.initials: E
authors.lastname: Oliveira
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), 08028 Barcelona, Spain; CIBER-BBN, Barcelona Science Park, 08028 Barcelona, Spain; School of Chemistry & Physics, University of KwaZulu-Natal, 4001 Durban, South Africa.
authors.forename: Fernando
authors.initials: F
authors.lastname: Albericio
authors.affiliation: Departamento de Química, Biotecnologia e Engenharia de Bioprocessos (DQBio), Universidade Federal de São João Del Rei, UFSJ, Campus Alto Paraopeba, Ouro Branco, MG, Brazil.
authors.forename: Saulo L
authors.initials: SL
authors.lastname: da Silva
authors.affiliation: Centro de Pesquisas Rene Rachou, FIOCRUZ Minas, Belo Horizonte, MG, Brazil.
authors.forename: Anna Caroline C
authors.initials: AC
authors.lastname: Aguiar
authors.affiliation: Centro de Pesquisas Rene Rachou, FIOCRUZ Minas, Belo Horizonte, MG, Brazil.
authors.forename: Antoniana U
authors.initials: AU
authors.lastname: Krettli
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, Fiocruz Rondônia e Programa de Pós Graduação em Biologia Experimental, PGBIOEXP, Núcleo de Saúde, NUSAU, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil.
authors.forename: Carla F C
authors.initials: CF
authors.lastname: Fernandes
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, Fiocruz Rondônia e Programa de Pós Graduação em Biologia Experimental, PGBIOEXP, Núcleo de Saúde, NUSAU, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil.
authors.forename: Juliana P
authors.initials: JP
authors.lastname: Zuliani
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, Fiocruz Rondônia e Programa de Pós Graduação em Biologia Experimental, PGBIOEXP, Núcleo de Saúde, NUSAU, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil.
authors.forename: Leonardo A
authors.initials: LA
authors.lastname: Calderon
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, Fiocruz Rondônia e Programa de Pós Graduação em Biologia Experimental, PGBIOEXP, Núcleo de Saúde, NUSAU, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil.
authors.forename: Rodrigo G
authors.initials: RG
authors.lastname: Stábeli
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, Fiocruz Rondônia e Programa de Pós Graduação em Biologia Experimental, PGBIOEXP, Núcleo de Saúde, NUSAU, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil. Electronic address: andreimar@fiocruz.br.
authors.forename: Andreimar M
authors.initials: AM
authors.lastname: Soares
mesh_terms: D000818:Animals; D000977:Antiparasitic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017837:Bothrops / Q000378:metabolism*; D002364:Caseins / Q000737:chemistry / Q000378:metabolism; D003435:Crotalid Venoms / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50; D008297:Male; D045726:Metalloproteases / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D010963:Plasmodium falciparum / Q000187:drug effects*; D013696:Temperature
chemical_list: D000977:Antiparasitic Agents; D002364:Caseins; D003435:Crotalid Venoms; C026018:azocasein; D005340:Fibrinogen; D045726:Metalloproteases
keywords: Antiplasmodial activity; B. brazili; Enzymatic characterization; Functional characterization; Metalloproteinase; Snake venom