title: CD39 expression on Treg and Th17 cells is associated with metabolic factors in patients with type 2 diabetes.
abstract: Th17 cells are involved in the pathogenesis of multiple inflammatory diseases such as type two diabetes (T2D). CD39(+) Treg cells have been implicated as responsible for suppressing Th17 cells. The aim of this study was to evaluate the number and function of CD4(+)CD25(high)CD39(+) Treg and Th17 cells in peripheral blood mononuclear cells (PBMC) from T2D patients and healthy control subjects. The Th17 cells were detected in PBMC under culture with human anti-CD3/CD28 and PMA/ionomycin and the levels of IL-17 were assessed by ELISA and qPCR. The T2D patients with obesity showed significantly lower percentages of CD39(+) Treg cells. A negative correlation between CD39(+) Treg cells and weight, and body mass index was detected. In contrast, the low levels of CD4(+)IL-17(+) cells in overweight and obese T2D patients showed a positive correlation with glucose and HbA1c. Additionally, we found a subpopulation of Th17 cells that express CD39 and were correlated with glucose and HbA1c. Our findings suggest that the expression of CD39 on Treg cells and also in CD4(+)IL-17(+) cells from T2D patients is related to hyperglycemia as well as to overweight and obesity and therefore may participate as a modulator of the effector capacity of Th17 cells.
authors.affiliation: Laboratory of Immunology and Cellular and Molecular Biology, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, SLP, Mexico.
authors.forename: Nancy
authors.initials: N
authors.lastname: Cortez-Espinosa
authors.affiliation: Laboratory of Immunology and Cellular and Molecular Biology, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, SLP, Mexico.
authors.forename: Juan Diego
authors.initials: JD
authors.lastname: Cortés-Garcia
authors.affiliation: Laboratory of Immunology and Cellular and Molecular Biology, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, SLP, Mexico.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Martínez-Leija
authors.affiliation: Department of Internal Medicine and Endocrinology, Hospital Central Dr. Ignacio Morones Prieto, San Luis Potosí, SLP, Mexico.
authors.forename: Jose Guillermo
authors.initials: JG
authors.lastname: Rodríguez-Rivera
authors.affiliation: Division of Molecular Biology, Instituto Potosino de Investigación Científica y Tecnológica, San Luis Potosí, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Barajas-López
authors.affiliation: Department of Immunology, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, SLP, Mexico.
authors.forename: Roberto
authors.initials: R
authors.lastname: González-Amaro
authors.affiliation: Laboratory of Immunology and Cellular and Molecular Biology, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, SLP, Mexico. Electronic address: dportale@uaslp.mx.
authors.forename: Diana Patricia
authors.initials: DP
authors.lastname: Portales-Pérez
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D001081:Apyrase / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism*; D058504:Th17 Cells / Q000276:immunology* / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D016207:Cytokines; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D001081:Apyrase; C067869:CD39 antigen
keywords: CD39; CD39(+) Treg cells; Obesity; Th17 cells; Type 2 diabetes