title: Rab and Arf G proteins in endosomal trafficking.
abstract: Endocytosis is a fundamental process that cells use to remove receptors, extracellular material, plasma membrane proteins and lipids from the cell surface. After entry into cells, the cargo proteins are subsequently trafficked to late endosomes and lysosomes for degradation, to the Golgi complex, or to recycling endosomes for return to the plasma membrane. Small G proteins in the Rab and Arf family are present on endosomes and coordinate the trafficking of cargo proteins. Here we describe some basic experimental approaches to begin to study the endosomal trafficking of a given cell surface protein.
authors.affiliation: Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Dipannita
authors.initials: D
authors.lastname: Dutta
authors.affiliation: Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Julie G
authors.initials: JG
authors.lastname: Donaldson
mesh_terms: D020727:ADP-Ribosylation Factors / Q000378:metabolism*; D011992:Endosomes / Q000201:enzymology*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D021381:Protein Transport; D020691:rab GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D020727:ADP-Ribosylation Factors; D020691:rab GTP-Binding Proteins
keywords: Arf6; Cargo sorting; Clathrin-independent endocytosis; Clathrin-mediated endocytosis; Endosomal recycling; G proteins; Immunofluorescence; Rab11; Rab22; Rab5