title: TRPV4 Channels in Human White Adipocytes: Electrophysiological Characterization and Regulation by Insulin.
abstract: Intracellular calcium homeostasis in adipocytes is important for the regulation of several functions and is involved in pathological changes in obesity and other associated diseases. Transient Receptor Potential Vanilloid 4 (TRPV4) channels are an important route for calcium entry that operates in a variety of cells and intervenes in a number of functions. In this study, the expression and operation of TRPV4 channels in human cultured adipocytes was evaluated using RT-PCR, Western blotting, the whole-cell patch-clamp technique and fluorescence measurements to characterize these channels and determine intracellular calcium responses. Both the hypoosmolarity and 4alpha-phorbol-didecanoate (4αPDD), a specific TRPV4 agonist, induced a similar HC-067047-sensitive current, which was predominantly inward, and an intracellular Ca(2+) concentration increase, which was exclusively dependent on extracellular calcium, and membrane depolarization. The current had a reverse potential of +31 ± 6 mV and exhibited preferential permeability to Ca(2+) . Insulin, which regulates metabolic homeostasis in adipocytes, attenuated the TRPV4-mediated effects. These results confirm the function of TRPV4 in human cultured adipocytes and its regulation by insulin. J. Cell. Physiol. 231: 954-963, 2016. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Facultad Ciencias de la Salud, Universidad Tecnológica de Pereira, Pereira, Colombia.
authors.forename: Julio C
authors.initials: JC
authors.lastname: Sánchez
authors.affiliation: Facultad Ciencias de la Salud, Universidad Tecnológica de Pereira, Pereira, Colombia.
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: Rivera
authors.affiliation: Facultad Ciencias de la Salud, Universidad Tecnológica de Pereira, Pereira, Colombia.
authors.forename: Laura V
authors.initials: LV
authors.lastname: Muñoz
mesh_terms: D052438:Adipocytes, White / Q000378:metabolism*; D002452:Cell Count; D002478:Cells, Cultured; D055724:Electrophysiological Phenomena / Q000187:drug effects*; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D015640:Ion Channel Gating / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism*
chemical_list: D007328:Insulin; D012333:RNA, Messenger; D050916:TRPV Cation Channels; C416986:TRPV4 protein, human