title: Evaluation of Minichromosome Maintenance Protein 7 and c-KIT as Prognostic Markers in Feline Cutaneous Mast Cell Tumours.
abstract: Mast cell tumours (MCTs) are a common skin tumour in cats, but there is currently no histological grading system or reliable prognostic marker for this species (unlike the situation for dogs). This study utilized a set of 71 feline cutaneous MCTs with known clinical outcomes to assess the potential of various prognostic markers, including the cellular proliferation marker minichromosome maintenance protein (MCM)-7, mitotic index and various KIT labelling characteristics, including KIT positivity, KIT labelling pattern and KIT immunoreactivity score (IS). Of the factors studied, the mitotic index and the KIT labelling pattern were the only features associated significantly with survival times, while the proliferation marker MCM7 and the KIT IS were not. The study also highlights the variability of KIT labelling characteristics between tumours, which may prevent use of this marker as a diagnostic and prognostic tool.
authors.affiliation: Finn Pathologists, One-Eyed Lane, Weybread, Diss, Norfolk, UK; Pathology and Pathogen Biology, Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire, UK. Electronic address: melanie.dobromylskyj@finnpathologists.com.
authors.forename: M J
authors.initials: MJ
authors.lastname: Dobromylskyj
authors.affiliation: Pathology Department, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk, UK.
authors.forename: R
authors.initials: R
authors.lastname: Rasotto
authors.affiliation: Pathology and Pathogen Biology, Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire, UK.
authors.forename: K
authors.initials: K
authors.lastname: Melville
authors.affiliation: Pathology and Pathogen Biology, Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire, UK.
authors.forename: K C
authors.initials: KC
authors.lastname: Smith
authors.affiliation: Oncology Unit, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk, UK.
authors.forename: D
authors.initials: D
authors.lastname: Berlato
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D002371:Cat Diseases / Q000378:metabolism / Q000473:pathology*; D002415:Cats; D005260:Female; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D034701:Mastocytosis, Cutaneous / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D064168:Minichromosome Maintenance Complex Component 7 / Q000032:analysis / Q000096:biosynthesis*; D011379:Prognosis; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; D019009:Proto-Oncogene Proteins c-kit; D064168:Minichromosome Maintenance Complex Component 7
keywords: cat; cutaneous mast cell tumour; immunohistochemistry; prognostic markers