title: Hormone signaling requirements for the conversion of non-mammary mouse cells to mammary cell fate(s) in vivo.
abstract: Mammotropic hormones and growth factors play a very important role in mammary growth and differentiation. Here, hormones including Estrogen, Progesterone, Prolactin, their cognate receptors, and the growth factor Amphiregulin, are tested with respect to their roles in signaling non-mammary cells from the mouse to redirect to mammary epithelial cell fate(s). This was done in the context of glandular regeneration in pubertal athymic female mice. Our previous studies demonstrated that mammary stem cell niches are recapitulated during gland regeneration in vivo. During this process, cells of exogenous origin cooperate with mammary epithelial cells to form mammary stem cell niches and thus respond to normal developmental signals. In all cases tested with the possible exception of estrogen receptor alpha (ER-α), hormone signaling is dispensable for non-mammary cells to undertake mammary epithelial cell fate(s), proliferate, and contribute progeny to chimeric mammary outgrowths. Importantly, redirected non-mammary cell progeny, regardless of their source, have the ability to self-renew and contribute offspring to secondary mammary outgrowths derived from transplanted chimeric mammary fragments; thus suggesting that some of these cells are capable of mammary stem cell/progenitor functions.
authors.affiliation: Mammary Stem Cell Biology Section, BRL, CCR, NCI, Bldg. 37 Rm. 1122A, Bethesda, MD, 20892, USA.
authors.forename: Corinne A
authors.identifier: http://orcid.org/0000-0003-4171-7588
authors.initials: CA
authors.lastname: Boulanger
authors.affiliation: Mammary Stem Cell Biology Section, BRL, CCR, NCI, Bldg. 37 Rm. 1122A, Bethesda, MD, 20892, USA.
authors.forename: Sonia M
authors.initials: SM
authors.lastname: Rosenfield
authors.affiliation: Mammary Stem Cell Biology Section, BRL, CCR, NCI, Bldg. 37 Rm. 1122A, Bethesda, MD, 20892, USA.
authors.forename: Andrea L
authors.initials: AL
authors.lastname: George
authors.affiliation: Mammary Stem Cell Biology Section, BRL, CCR, NCI, Bldg. 37 Rm. 1122A, Bethesda, MD, 20892, USA. gs4d@nih.gov.
authors.forename: Gilbert H
authors.initials: GH
authors.lastname: Smith
mesh_terms: D066258:Amphiregulin / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000254:growth & development* / Q000378:metabolism*; D051379:Mice; D011374:Progesterone / Q000378:metabolism; D011388:Prolactin / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D015398:Signal Transduction; D013234:Stem Cells / Q000378:metabolism* / Q000502:physiology
chemical_list: D066258:Amphiregulin; D004967:Estrogens; D011980:Receptors, Progesterone; D011374:Progesterone; D011388:Prolactin
keywords: Amphiregulin; Estrogen; Hormone receptor; Mammary; Niche; Progesterone; Prolactin