title: Magnetic Particle Imaging tracks the long-term fate of in vivo neural cell implants with high image contrast.
abstract: We demonstrate that Magnetic Particle Imaging (MPI) enables monitoring of cellular grafts with high contrast, sensitivity, and quantitativeness. MPI directly detects the intense magnetization of iron-oxide tracers using low-frequency magnetic fields. MPI is safe, noninvasive and offers superb sensitivity, with great promise for clinical translation and quantitative single-cell tracking. Here we report the first MPI cell tracking study, showing 200-cell detection in vitro and in vivo monitoring of human neural graft clearance over 87 days in rat brain.
authors.affiliation: Department of Bioengineering, University of California at Berkeley, Berkeley, CA 94720, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.affiliation: Department of Bioengineering, University of California at Berkeley, Berkeley, CA 94720, USA.|Department of Chemical and Biomolecular Engineering, University of California at Berkeley, Berkeley, CA 94720, USA.|Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA.
authors.forename: Tandis
authors.initials: T
authors.lastname: Vazin
authors.affiliation: Department of Bioengineering, University of California at Berkeley, Berkeley, CA 94720, USA.|Magnetic Insight, Inc., Newark, CA 94560, USA.
authors.forename: Patrick W
authors.initials: PW
authors.lastname: Goodwill
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California at Berkeley, Berkeley, CA 94720, USA.
authors.forename: Anthony
authors.initials: A
authors.lastname: Conway
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California at Berkeley, Berkeley, CA 94720, USA.
authors.forename: Aradhana
authors.initials: A
authors.lastname: Verma
authors.affiliation: Department of Bioengineering, University of California at Berkeley, Berkeley, CA 94720, USA.
authors.forename: Emine Ulku
authors.initials: EU
authors.lastname: Saritas
authors.affiliation: Department of Bioengineering, University of California at Berkeley, Berkeley, CA 94720, USA.|Department of Chemical and Biomolecular Engineering, University of California at Berkeley, Berkeley, CA 94720, USA.|Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA.
authors.forename: David
authors.initials: D
authors.lastname: Schaffer
authors.affiliation: Department of Bioengineering, University of California at Berkeley, Berkeley, CA 94720, USA.|Department of Electrical Engineering and Computer Science, University of California at Berkeley, Berkeley, CA 94720, USA.
authors.forename: Steven M
authors.initials: SM
authors.lastname: Conolly
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D058948:Cell Tracking / Q000379:methods; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D006801:Humans; D007501:Iron / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods; D058185:Magnetite Nanoparticles; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
chemical_list: D058185:Magnetite Nanoparticles; D007501:Iron