title: Proteomics of Aggregatibacter actinomycetemcomitans Outer Membrane Vesicles.
abstract: Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive forms of periodontitis and with endocarditis. Outer membrane vesicles (OMVs) released by this species have been demonstrated to deliver effector proteins such as cytolethal distending toxin (CDT) and leukotoxin (LtxA) into human host cells and to act as triggers of innate immunity upon carriage of NOD1- and NOD2-active pathogen-associated molecular patterns (PAMPs). To improve our understanding of the pathogenicity-associated functions that A. actinomycetemcomitans exports via OMVs, we studied the proteome of density gradient-purified OMVs from a rough-colony type clinical isolate, strain 173 (serotype e) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). This analysis yielded the identification of 151 proteins, which were found in at least three out of four independent experiments. Data are available via ProteomeXchange with identifier PXD002509. Through this study, we not only confirmed the vesicle-associated release of LtxA, and the presence of proteins, which are known to act as immunoreactive antigens in the human host, but we also identified numerous additional putative virulence-related proteins in the A. actinomycetemcomitans OMV proteome. The known and putative functions of these proteins include immune evasion, drug targeting, and iron/nutrient acquisition. In summary, our findings are consistent with an OMV-associated proteome that exhibits several offensive and defensive functions, and they provide a comprehensive basis to further disclose roles of A. actinomycetemcomitans OMVs in periodontal and systemic disease.
authors.affiliation: Department of Chemistry, Umeå University, Umeå, Sweden.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kieselbach
authors.affiliation: Center for Dentistry and Oral Hygiene, University Medical Center Groningen, Groningen, The Netherlands.
authors.forename: Vincent
authors.initials: V
authors.lastname: Zijnge
authors.affiliation: Oral Microbiology, Department of Odontology, Umeå University, Umeå, Sweden.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Granström
authors.affiliation: Oral Microbiology, Department of Odontology, Umeå University, Umeå, Sweden.
authors.forename: Jan
authors.initials: J
authors.lastname: Oscarsson
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D006801:Humans; D010518:Periodontitis / Q000382:microbiology*; D040901:Proteomics; D022142:Secretory Vesicles / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins