title: RNA interference machinery-mediated gene regulation in mouse adult neural stem cells.
abstract: BACKGROUND
  Neurogenesis in the brain of adult mammals occurs throughout life in two locations: the subventricular zone of the lateral ventricle and the subgranular zone of the dentate gyrus in the hippocampus. RNA interference mechanisms have emerged as critical regulators of neuronal differentiation. However, to date, little is known about its function in adult neurogenesis.
  
  
  RESULTS
  Here we show that the RNA interference machinery regulates Doublecortin levels and is associated with chromatin in differentiating adult neural progenitors. Deletion of Dicer causes abnormal higher levels of Doublecortin. The microRNA pathway plays an important role in Doublecortin regulation. In particular miRNA-128 overexpression can reduce Doublecortin levels in differentiating adult neural progenitors.
  
  
  CONCLUSIONS
  We conclude that the RNA interference components play an important role, even through chromatin association, in regulating neuron-specific gene expression programs.
authors.affiliation: Research Group Adult Neurogenesis and Neural Stem Cells, Institute of Developmental Genetics, Helmholtz Center Munich, German Research Center for Environmental Health, Munich-Neuherberg, Germany. filippo.cernilogar@med.uni-muenchen.de.|Biomedical Center, Ludwig Maximilian University, Großhaderner Strasse 9, 82152, Planegg-Martinsried, Germany. filippo.cernilogar@med.uni-muenchen.de.
authors.forename: Filippo M
authors.initials: FM
authors.lastname: Cernilogar
authors.affiliation: Institute for Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Munich-Neuherberg, Germany. digiaimo@unina.it.|Department of Biology, University of Naples Federico II, Naples, Italy. digiaimo@unina.it.
authors.forename: Rossella
authors.initials: R
authors.lastname: Di Giaimo
authors.affiliation: Research Group Adult Neurogenesis and Neural Stem Cells, Institute of Developmental Genetics, Helmholtz Center Munich, German Research Center for Environmental Health, Munich-Neuherberg, Germany. Frederick.Rehfeld@charite.de.|Institute of Cell Biology and Neurobiology, Charité University, Berlin, Germany. Frederick.Rehfeld@charite.de.
authors.forename: Frederick
authors.initials: F
authors.lastname: Rehfeld
authors.affiliation: Developmental Neurobiology, Max Planck Institute of Psychiatry, Munich, Germany. silvia_cappello@psych.mpg.de.
authors.forename: Silvia
authors.initials: S
authors.lastname: Cappello
authors.affiliation: Research Group Adult Neurogenesis and Neural Stem Cells, Institute of Developmental Genetics, Helmholtz Center Munich, German Research Center for Environmental Health, Munich-Neuherberg, Germany. chi.lie@fau.de.|Institute of Biochemistry, Emil Fischer Center, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany. chi.lie@fau.de.
authors.forename: D Chichung
authors.initials: DC
authors.lastname: Lie
mesh_terms: D000818:Animals; D002843:Chromatin / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D000089622:Doublecortin Domain Proteins; D015870:Gene Expression / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D035683:MicroRNAs / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D058953:Neural Stem Cells / Q000378:metabolism*; D055495:Neurogenesis / Q000502:physiology*; D009479:Neuropeptides / Q000378:metabolism*; D034622:RNA Interference / Q000502:physiology*; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism*
chemical_list: D002843:Chromatin; D000089622:Doublecortin Domain Proteins; D035683:MicroRNAs; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; C509781:Dicer1 protein, mouse; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases