title: Data from a proteomic baseline study of Assemblage A in Giardia duodenalis.
abstract: Eight Assemblage A strains from the protozoan parasite Giardia duodenalis were analysed using label-free quantitative shotgun proteomics, to evaluate inter- and intra-assemblage variation and complement available genetic and transcriptomic data. Isolates were grown in biological triplicate in axenic culture, and protein extracts were subjected to in-solution digest and online fractionation using Gas Phase Fractionation (GPF). Recent reclassification of genome databases for subassemblages was evaluated for database-dependent loss of information, and proteome composition of different isolates was analysed for biologically relevant assemblage-independent variation. The data from this study are related to the research article "Quantitative proteomics analysis of Giardia duodenalis Assemblage A - a baseline for host, assemblage and isolate variation" published in Proteomics (Emery et al., 2015 [1]).
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, North Ryde, NSW 2109, Australia.
authors.forename: Samantha J
authors.initials: SJ
authors.lastname: Emery
authors.affiliation: Microbial Screening Technologies Pty Ltd, Smithfield, NSW 2165, Australia.
authors.forename: Ernest
authors.initials: E
authors.lastname: Lacey
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, North Ryde, NSW 2109, Australia.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Haynes
keywords: Assemblage A; Giardia duodenalis; Label-free quantitative shotgun proteomics; Parasite proteomics; Variable genome; Variant Surface Protein