title: A suggested guiding panel of seromarkers for efficient discrimination between primary and secondary human hepatocarcinoma.
abstract: Although alpha-fetoprotein (AFP) is a golden diagnostic marker for hepatocellular carcinoma (HCC), its value is debatable. Differentiation between primary and secondary hepatocarcinomas (HC) relying on AFP is confusing, does not exceed 20 % in the later. To find alternative markers other than AFP to differentiate between primary and secondary HC from colorectal carcinoma (CRC) and breast (BC) and lung cancers (LC), 60 individuals were recruited: group 1, healthy volunteers; group 2, with primary; and group 3, with secondary HC. Carcinoembryonic antigen (CEA), total glycosaminoglycans (TGAGs), total sialic acid (TSA), free glucosamine (FGA), leucine aminopeptidase (LAP), 5'-nucleotidase (5'-NU) activities, and AFP were estimated in sera, in addition to liver histology. CEA, TGAGs, TSA, and FGA were elevated in secondary HC among CRC primary cancers, while LAP, 5'-NU activities, and AFP were elevated in primary HCC. We concluded that a new panel can be used to differentiate primary from secondary HC better than AFP, speculating the primary cancer. AFP, LAP, and 5'-NU predominated in primary, while CEA, TGAGs, TSA, and FGA, in secondary HC. Elevation of 5'-NU, LAP, TGAGs, TSA, and FGA to CEA indicated that primary source of HC is CRC. Association of TGAGs, TSA, and FGA only to CEA indicated that the primary cancer is breast. Elevation of TGAGs, TSA, and FGA, with other normal parameters, indicated that the primary cancer is lung. A guiding table is recommended in the oncology laboratory, for management and follow-up, and having more expected level of sensitivity than AFP.
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh, Egypt. nabilmohie@yahoo.com.
authors.forename: Nabil Mohie
authors.initials: NM
authors.lastname: Abdel-Hamid
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
authors.forename: M M
authors.initials: MM
authors.lastname: Abouzied
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
authors.forename: M H
authors.initials: MH
authors.lastname: Nazmy
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
authors.forename: M A
authors.initials: MA
authors.lastname: Fawzy
authors.affiliation: Oncology Department, College of Medicine, Minia University, Minia, Egypt.
authors.forename: A S
authors.initials: AS
authors.lastname: Gerges
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology*; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D012680:Sensitivity and Specificity; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D000509:alpha-Fetoproteins
keywords: Alpha-fetoprotein glycosaminoglycans; Hepatocellular carcinoma; Liver metastasis; Sialic acid