title: Kiloniella litopenaei sp. nov., isolated from the gut microflora of Pacific white shrimp, Litopenaeus vannamei.
abstract: A cream-coloured rod-shaped bacterium, designated strain P1-1(T), was isolated from the gut microflora of Pacific white shrimp (Litopenaeus vannamei) from the Taiwan Strait, China. P1-1(T) was was found to be Gram-negative, oxidase and catalase positive and motile by means of a single flagellum. Growth is observed at salinities of 0.5-7 % (w/v; optimum 3 %), at pH 6.0-10.0 (optimum pH 7.0-9.0) and at 15-37 °C (optimum 28-32 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P1-1(T) belongs to the genus Kiloniella, and is closely related to Kiloniella spongiae MEBiC09566(T) (99.0 % similarity), followed by Kiloniella laminariae LD81(T) (96.6 % similarity). The genomic ANIm value between strain P1-1(T) and K. spongiae MEBiC09566(T) was found to be 81.7 %. The DNA-DNA hybridization estimate between strains P1-1(T) and MEBiC09566(T) was 24.6 %. The principal fatty acids detected were identified as summed feature 8 (C18:1 ω6c/C18:1 ω7c, 74.2 %) and summed feature 3 (C16:1 ω6c/C16:1 ω7c, 9.7 %). The G+C content of the chromosomal DNA, determined from the draft genome sequence, was found to be 46.1 mol%. The major respiratory quinone was determined to be Q-9, while the major polar lipids are phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids, one unidentified aminolipid and nine unidentified lipids. The combined genotypic and phenotypic data indicate that strain P1-1(T) represents a novel species within the genus Kiloniella, for which the name Kiloniella litopenaei sp. nov. is proposed, with the type strain P1-1(T) (=MCCC 1A09869(T) = LMG 27755(T)).
authors.affiliation: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150000, China.|State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361005, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361005, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361005, China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.affiliation: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150000, China. shaozz@163.com.|State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361005, China. shaozz@163.com.
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041981:Gastrointestinal Tract / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D033561:Penaeidae / Q000382:microbiology*; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride
keywords: Average nucleotide identity; DNA–DNA hybridization; Kiloniella litopenaei sp. nov.; Litopenaeus vannamei; Taxonomy