title: Immunomodulatory Effect of β-Glucan on Peritoneal Macrophages of Bab1/c Mice.
abstract: We assessed the effect of β-Glucan on macrophages by Griess reagent and viability by MTT assay and cytotoxicity. Assay of macrophages culture supernatants were carried out on WEHI-164 fibrosarcoma cell line as tumor necrosis factor-α bioassay were done. NO release was increased at the dose of 10 µg/ml (P = 0.001) of β-Glucan while the viability of macrophages in all concentrations was the same. In TNF-α bioassay, the supernatant of macrophages stimulated with β-Glucan had a significant cytotoxic effect on WEHI-164 cells (P = 0.023). β-Glucan had a positive effect on increasing tumoricidal activity of macrophages which may help in anti-cancer immune responses.
authors.forename: Maryam
authors.initials: M
authors.lastname: Roudbary
authors.forename: Saeed
authors.initials: S
authors.lastname: Daneshmand
authors.forename: Monire
authors.initials: M
authors.lastname: Hajimorad
authors.forename: Shahla
authors.initials: S
authors.lastname: Roudbarmohammadip
authors.forename: Zuhair M
authors.initials: ZM
authors.lastname: Hassan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D047071:beta-Glucans / Q000494:pharmacology*
chemical_list: D047071:beta-Glucans