title: Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites.
abstract: We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions.
  
  
  METHODS
  Free access at http://rgenome.net/cas-designer/.
authors.affiliation: Department of Chemistry.
authors.forename: Jeongbin
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Chemistry, Institute for Materials Design, Hanyang University, Seoul, South Korea.
authors.forename: Sangsu
authors.initials: S
authors.lastname: Bae
authors.affiliation: Center for Genome Engineering, Institute for Basic Science, Seoul, South Korea and Department of Chemistry, Seoul National University, Seoul, South Korea.
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Kim
mesh_terms: D064130:CRISPR-Associated Proteins; D064113:CRISPR-Cas Systems; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D004706:Endodeoxyribonucleases; D055786:Gene Knockout Techniques; D016678:Genome; D020407:Internet; D012313:RNA / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software
chemical_list: D064130:CRISPR-Associated Proteins; D012313:RNA; D004706:Endodeoxyribonucleases