title: Functional Characterization of Hevea brasiliensis CRT/DRE Binding Factor 1 Gene Revealed Regulation Potential in the CBF Pathway of Tropical Perennial Tree.
abstract: Rubber trees (Hevea brasiliensis) are susceptible to low temperature and therefore are only planted in the tropical regions. In the past few decades, although rubber trees have been successfully planted in the northern margin of tropical area in China, they suffered from cold injury during the winter. To understand the physiological response under cold stress, we isolated a C-repeat binding factor 1 (CBF1) gene from the rubber tree. This gene (HbCBF1) was found to respond to cold stress but not drought or ABA stress. The corresponding HbCBF1 protein showed CRT/DRE binding activity in gel shift experiment. To further characterize its molecular function, the HbCBF1 gene was overexpressed in Arabidopsis. The HbCBF1 over expression (OE) line showed enhanced cold resistance and relatively slow dehydration, and the expression of Arabidopsis CBF pathway downstream target genes, e.g. AtCOR15a and AtRD29a, were significantly activated under non-acclimation condition. These data suggest HbCBF1 gene is a functional member of the CBF gene family, and may play important regulation function in rubber tree.
authors.affiliation: Key Laboratory of Rubber Biology, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Science, Danzhou, Hainan, P.R. China.
authors.forename: Han
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Rubber Biology, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Science, Danzhou, Hainan, P.R. China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Cai
authors.affiliation: Guangxi Subtropical Crops Research Institute, Nanning, Guangxi, P.R. China.
authors.forename: Haitian
authors.initials: H
authors.lastname: Fu
authors.affiliation: Key Laboratory of Rubber Biology, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Science, Danzhou, Hainan, P.R. China.
authors.forename: Zewei
authors.initials: Z
authors.lastname: An
authors.affiliation: Key Laboratory of Rubber Biology, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Science, Danzhou, Hainan, P.R. China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Fang
authors.affiliation: Key Laboratory of Rubber Biology, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Science, Danzhou, Hainan, P.R. China.
authors.forename: Yanshi
authors.initials: Y
authors.lastname: Hu
authors.affiliation: School of Life Science and the State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, PR China.
authors.forename: Dianjing
authors.initials: D
authors.lastname: Guo
authors.affiliation: Key Laboratory of Rubber Biology, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Science, Danzhou, Hainan, P.R. China.
authors.forename: Huasun
authors.initials: H
authors.lastname: Huang
mesh_terms: D000064:Acclimatization; D000595:Amino Acid Sequence; D001483:Base Sequence; D058639:Cold-Shock Response; D028482:Hevea / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D015534:Trans-Activators