title: The influence of N-acetyl-l-cysteine on damage of porcine oocyte exposed to zearalenone in vitro.
abstract: Zearalenone (ZEA), one of the mycotoxins produced by Fusarium fungi, impacts porcine reproduction by interfering with the estrogen signaling pathway. Previous studies have shown that ZEA inhibits porcine oocyte maturation through the formation of aberrant spindle. To explore the effect of ZEA on porcine oocyte meiotic maturation, the extent of both nuclear and cytoplasmic maturation was examined in this study. Compared with control group, presence of ZEA (3 μM) during oocyte maturation, significantly inhibited the polar body extrusions from 71% to 51%, and significantly increased intracellular reactive oxygen species (ROS) level (12.01 vs. 5.89). Intracellular glutathione (GSH) content in ZEA treatment group was lower than in the control group (1.08 pmol/oocyte vs. 0.18 pmol/oocyte), and cortical granules of cortical area distributed oocytes were reduced (88% vs. 62%). ZEA decreases cumulus expansion in both morphology and mRNA level (HAS2, PTX3, TNFAIP6 and CX43). Addition of N-acetyl-l-cysteine (NAC) to the oocyte maturation media reversed the ZEA-induced inhibition of polar body extrusion (from 69% to 81%), up-regulated ROS (from 7.9 to 6.5), down-regulated GSH content (from 0.16 to 0.82 pmol/oocyte) and recovered cumulus cells expansion in morphology and mRNA level. It is concluded that ZEA affects both oocyte nucleus and cytoplasmic maturation during in vitro maturation, and NAC can reverse these damages to some extent.
authors.affiliation: College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China; Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Fang-Nong
authors.initials: FN
authors.lastname: Lai
authors.affiliation: Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao 266109, China; Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Jun-Yu
authors.initials: JY
authors.lastname: Ma
authors.affiliation: College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China; Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Jing-Cai
authors.initials: JC
authors.lastname: Liu
authors.affiliation: Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao 266109, China; Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao 266109, China; Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Shun-Feng
authors.initials: SF
authors.lastname: Cheng
authors.affiliation: College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China; Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Sun
authors.affiliation: Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao 266109, China; Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.affiliation: Chengguo Station of Animal Husbandry and Veterinary, Laizhou 261437, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Charles Martin
authors.initials: CM
authors.lastname: Nyachoti
authors.affiliation: Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao 266109, China; Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China. Electronic address: wshen@qau.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D018031:Connexin 43 / Q000235:genetics / Q000378:metabolism; D019610:Cytoprotection; D005260:Female; D018507:Gene Expression Regulation, Developmental; D014453:Glucuronosyltransferase / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D059471:In Vitro Oocyte Maturation Techniques; D008540:Meiosis / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D000683:Serum Amyloid P-Component / Q000235:genetics / Q000378:metabolism; D013552:Swine; D015025:Zearalenone / Q000633:toxicity*; D000099153:Pentraxins
chemical_list: D000111:Acetylcysteine; D000975:Antioxidants; D002097:C-Reactive Protein; D018031:Connexin 43; D014453:Glucuronosyltransferase; D005978:Glutathione; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D000683:Serum Amyloid P-Component; D015025:Zearalenone; D000099153:Pentraxins
keywords: Cumulus cells expansion; Maturation in vitro; N-acetyl-l-cysteine; Oocyte; Porcine; Zearalenone