title: Mass spectrometry guided venom profiling and bioactivity screening of the Anatolian Meadow Viper, Vipera anatolica.
abstract: This contribution reports on the first characterization of the venom proteome and the bioactivity screening of Vipera anatolica, the Anatolian Meadow Viper. The crude venom as well as an isolated dimeric disintegrin showed remarkable cytotoxic activity against glioblastoma cells. Due to the rare occurrence and the small size of this species only little amount of venom was available, which was profiled by means of a combination of bottom-up and top-down mass spectrometry. From this analysis we identified snake venom metalloproteases, cysteine-rich secretory protein isoforms, a metalloprotease inhibitor, several type A2 phospholipases, disintegrins, a snake venom serine protease, a C-type lectin and a Kunitz-type protease inhibitor. Furthermore, we detected several isoforms of above mentioned proteins as well as previously unknown proteins, indicating an extensive complexity of the venom which would have remained undetected with conventional venomic approaches.
authors.affiliation: Zoology Section, Department of Biology, Faculty of Science, Ege University, 35100 Bornova, Izmir, Turkey.
authors.forename: Bayram
authors.initials: B
authors.lastname: Göçmen
authors.affiliation: Technische Universität Berlin, Institut für Chemie, Strasse des 17. Juni 124, 10623 Berlin, Germany.
authors.forename: Paul
authors.initials: P
authors.lastname: Heiss
authors.affiliation: Technische Universität Berlin, Institut für Chemie, Strasse des 17. Juni 124, 10623 Berlin, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Petras
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, 35100 Izmir, Turkey. Electronic address: analbantsoy@gmail.com.
authors.forename: Ayse
authors.initials: A
authors.lastname: Nalbantsoy
authors.affiliation: Technische Universität Berlin, Institut für Chemie, Strasse des 17. Juni 124, 10623 Berlin, Germany. Electronic address: roderich.suessmuth@tu-berlin.de.
authors.forename: Roderich D
authors.initials: RD
authors.lastname: Süssmuth
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D019483:Disintegrins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry / Q000302:isolation & purification; D061986:MCF-7 Cells; D013058:Mass Spectrometry / Q000379:methods*; D045726:Metalloproteases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D054467:Phospholipases A2 / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011480:Protease Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020033:Protein Isoforms / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D030162:Reptilian Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D057057:Serine Proteases / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014757:Viper Venoms / Q000737:chemistry*; D017819:Viperidae
chemical_list: D019483:Disintegrins; D037181:Lectins, C-Type; D011480:Protease Inhibitors; D020033:Protein Isoforms; D030162:Reptilian Proteins; D014757:Viper Venoms; D054467:Phospholipases A2; D045726:Metalloproteases; D057057:Serine Proteases
keywords: Anatolian Meadow Viper; Bioactivity screening; Cytotoxicity; Snake venomics; Top-down venomics; Vipera anatolica; Viperidae