title: Evaluation of the effects of systemic photodynamic therapy in a rat model of acute myeloid leukemia.
abstract: Systemic PDT (SPDT) approach is developed to treat a variety of hematological diseases, including cancers and blood-borne infections. We evaluated the efficacy of an SPDT method for treating leukemia using a Brown Norway myeloid leukemia (BNML) rat model with the LT12 cells engineered to express GFP. The survival times of animals receiving SPDT at 5 (early-SPDT) and 10 (mid-SPDT) days post-LT12 injection were prolonged by 2 days, the rats in the late-SPDT group (15 days) exhibited a 6-day increase in life span (p<0.05). The percentages of GFP-LT12 cells in the bone marrow of the late-SPDT rats decreased from 61.6% to 56.5% on day 17. Likewise, there was a decrease in the serum expression levels of IL-1β, IL-10, TNF-α, and IFN-γ in the late-SPDT rats (p<0.05). Our findings indicate that SPDT could be an effective method for the treatment of leukemia, and that antitumor immunity may play a key role in this process.
authors.affiliation: Lab of Laser Medicine, Institute of Medical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Yin
authors.affiliation: Cardiovascular Department, Tianjin First Central Hospital, Tianjin 300192, China.
authors.forename: Xuying
authors.initials: X
authors.lastname: Ye
authors.affiliation: Lab of Laser Medicine, Institute of Medical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China. Electronic address: yingxinli2005@126.com.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Lab of Laser Medicine, Institute of Medical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Niu
authors.affiliation: Biomedical Engineering College, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Tianjin Medical University Eye Hospital, Tianjin 300384, China.
authors.forename: Wenjiang
authors.initials: W
authors.lastname: Ma
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D045744:Cell Line, Tumor; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D057809:HEK293 Cells; D006415:Hematoporphyrins / Q000627:therapeutic use; D006801:Humans; D054023:Lasers, Semiconductor; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000627:therapeutic use*; D051381:Rats; D011914:Rats, Inbred BN; D014184:Transplantation, Homologous
chemical_list: D016207:Cytokines; D006415:Hematoporphyrins; D017319:Photosensitizing Agents; C494379:hematoporphyrin monomethyl ether
keywords: Cytokines; Extracorporeal circulation; GFP; Leukemia; Photodynamic therapy