title: [In vitro study on the properties of guided bone regeneration of new type chitosan-based thermosensitive hydrogel membranes].
abstract: OBJECTIVE
  To prepare chitosan/β-glycerophosphate salt (CS/β-GP) thermosensitive hydrogel membranes loaded enamel matrix proteins and detect the guided bone regeneration properties.
  
  
  METHODS
  A newly membrane was synthesized using thermal phase inversion property of the CS/ββ-GP system. The membrane was synthesized and added with protein BSA. The concentration of protein was detected at different time points by enhanced protein assay kit, and the protein release curve was drawn. CS/β-GP membrane added EMPs (1.0 g) as group A, CS/β-GP (1.0 g) membrane as group B and nothing as blank control group (group C). They were co-cultured with ST2 cells. The mechanical properties of the membranes were tested in vitro, and the compatibility properties were detected by MTT method. The activity of ALP was assayed by PNPP method. The data was analyzed by SPSS 16.0 software package.
  
  
  RESULTS
  Membranes with different concentration of CS/β-GP could release protein slowly more than 12 days, and the total quantity of the released protein increased with the concentration of the β-GP. The changes of mechanical properties of the membranes were not significant (P>0.05). The OD value of group A, B and C had statistically significant difference in the MTT test. The values of group A and B were higher than that of group C, while the value of group A was higher than that of group B (P<0.05). The activities of ALP were different in the three groups. The activities of group A and B were higher than that of the blank control group (P<0.05).The difference in expression of ALP between group A and B was also statistically significant. The expression in group A was higher than that in group B (P<0.05).
  
  
  CONCLUSIONS
  The new type CS/β-GP membrane shows property of guided bone regeneration in vitro, which have the potentials for clinical use.
authors.affiliation: Yantai Stomatological Hospital. Yantai 264001;Shandong Province, China. E-mail:kathyliangjie@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.forename: Jun
authors.initials: J
authors.lastname: Cui
authors.forename: Sheng
authors.initials: S
authors.lastname: Xu
authors.forename: Ti
authors.initials: T
authors.lastname: Zhou
authors.forename: Wei-cheng
authors.initials: WC
authors.lastname: Xu
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
mesh_terms: D001861:Bone Regeneration; D048271:Chitosan / Q000737:chemistry*; D003746:Dental Enamel Proteins; D005994:Glycerophosphates / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*
chemical_list: D003746:Dental Enamel Proteins; D005994:Glycerophosphates; C109069:enamel matrix proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan; C031463:beta-glycerophosphoric acid