title: Pretreatment optimization of Sorghum pioneer biomass for bioethanol production and its scale-up.
abstract: Based on one parameter at a time, saccharification of delignified sorghum biomass by 4% and 70% v/v sulfuric acid resulted in maximum 30.8 and 33.8 g% sugar production from biomass respectively. The Box Behnken Design was applied for further optimization of acid hydrolysis. As a result of the designed experiment 36.3g% sugar production was achieved when 3% v/v H2SO4 treatment given for 60 min at 180°C. The process was scaled-up to treat 2 kg of biomass. During the screening of yeast cultures, isolate C, MK-I and N were found to be potent ethanol producers from sorghum hydrolyzate. Culture MK-I was the best so used for scale up of ethanol production up to 25 L capacity, which gave a yield of 0.49 g ethanol/g sugar from hydrolyzate obtained from 2 kg of sorghum biomass.
authors.affiliation: Department of Microbiology, Biogas Research Centre, Gujarat Vidhyapith, Sadra 382424, Gujarat, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Koradiya
authors.affiliation: Department of Microbiology, Biogas Research Centre, Gujarat Vidhyapith, Sadra 382424, Gujarat, India.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Duggirala
authors.affiliation: Department of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad 380 009, Gujarat, India.
authors.forename: Devayani
authors.initials: D
authors.lastname: Tipre
authors.affiliation: Department of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad 380 009, Gujarat, India. Electronic address: shaileshrdave@yahoo.co.in.
authors.forename: Shailesh
authors.initials: S
authors.lastname: Dave
mesh_terms: D000143:Acids; D018533:Biomass; D002241:Carbohydrates / Q000737:chemistry; D002244:Carbon; D000431:Ethanol / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D009584:Nitrogen; D045868:Sorghum / Q000737:chemistry*; D013464:Sulfuric Acids; D015003:Yeasts / Q000378:metabolism
chemical_list: D000143:Acids; D002241:Carbohydrates; D013464:Sulfuric Acids; D000431:Ethanol; D002244:Carbon; D009584:Nitrogen; C033158:sulfuric acid
keywords: Acid hydrolysis; Bioethanol; Box Behnken Design; Saccharification; Sorghum stover biomass