title: Effect of teriparatide on repair of femoral metaphyseal defect in ovariectomized rats.
abstract: OBJECTIVE
  This study aimed to investigate the effect exerted by teriparatide on the repair of femoral metaphyseal defect in ovariectomized rats.
  
  
  METHODS
  Female Sprague-Dawley rats were ovariectomized and after 3 months a critically sized defect of 3 mm in diameter-a through-hole bone defect-was drilled into each distal femur of the ovariectomized rats. The rats were injected with teriparatide (30 μg/kg) parathyroid hormone (PTH) in the peritoneum three times per week. After 4 and 8 weeks the animals were killed and the blood and bilateral femora were harvested for biochemical analysis, histopathological observation, and micro-computed tomography (CT) examination.
  
  
  RESULTS
  The PTH group and control group were compared 4  and 8 weeks after surgery. PTH increased bone formation in the defect area. Moreover, PTH showed the strongest effects on bone volume per total volume, trabecular number, trabecular thickness, trabecular separation, and total fluorescence-marked new bone area. Additionally, the PTH treatment group showed inhibited serum concentrations of C-terminal telopeptide of type I collagen and enhanced expression of calcium, phosphorus, and bone alkaline phosphatase.
  
  
  CONCLUSIONS
  Our findings suggest a positive effect of PTH on defect healing in ovariectomized rats.
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 325027, Wenzhou, People's Republic of China.
authors.forename: Zhou-Shan
authors.initials: ZS
authors.lastname: Tao
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 325027, Wenzhou, People's Republic of China.
authors.forename: Yang-Xun
authors.initials: YX
authors.lastname: Lv
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 325027, Wenzhou, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 325027, Wenzhou, People's Republic of China.
authors.forename: Zheng-Liang
authors.initials: ZL
authors.lastname: Huang
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 325027, Wenzhou, People's Republic of China.
authors.forename: Kai-Kai
authors.initials: KK
authors.lastname: Tu
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 325027, Wenzhou, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 325027, Wenzhou, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 325027, Wenzhou, People's Republic of China. tzs19900327@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
mesh_terms: D000818:Animals; D050071:Bone Density Conservation Agents / Q000627:therapeutic use; D001861:Bone Regeneration / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D005260:Female; D005264:Femoral Fractures / Q000000981:diagnostic imaging* / Q000188:drug therapy* / Q000473:pathology; D017102:Fracture Healing / Q000187:drug effects*; D058866:Osteoporotic Fractures / Q000000981:diagnostic imaging* / Q000188:drug therapy* / Q000473:pathology; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D019379:Teriparatide / Q000008:administration & dosage*; D014057:Tomography, X-Ray Computed / Q000379:methods; D016896:Treatment Outcome
chemical_list: D050071:Bone Density Conservation Agents; D019379:Teriparatide
keywords: Bone defect; Bone mineralization; Micro-computed tomography; New bone; Osteoporosis