title: EFFECT OF CYP2A6*4 GENETIC POLYMORPHISMS ON SMOKING BEHAVIORS AND NICOTINE DEPENDENCE IN A GENERAL POPULATION OF JAPANESE MEN.
abstract: OBJECTIVE
  Nicotine in cigarettes is metabolized primarily by CYP2A6-catalyzed oxidation. The CYP2A6*4 allele, in which CYP2A6 is a homozygous whole-deletion variant, completely lacks enzyme activity. The aim of this study was to examine the effects of CYP2A6*4 genetic polymorphism on smoking behavior and nicotine dependence in a general population of Japanese men.
  
  
  METHODS
  The subjects were 124 healthy Japanese men who gave informed consent to give saliva samples. The survey items included general information, smoking behaviors and nicotine dependence. The polymerase chain reaction restriction fragment length polymorphism method was used to analyze the genetic polymorphisms of CYP2A6. The subjects were classified into two groups: Group W (CYP2A6*4 absence: *1A/*1A, *1A/*1B and *1B/*1B) and Group D (CYP2A6*4 presence: *1B/*4A, *4A/*4A, *1A/*4A or *1B/*4D, and *1A/*4D). We analyzed the differences in the survey items between the two groups.
  
  
  RESULTS
  There were no significant differences in smoking behaviors between the two groups. However, Group D tended to have less difficulty in refraining from smoking after waking in the morning compared to Group W (p=0.051).
  
  
  CONCLUSIONS
  CYP2A6*4 genetic polymorphisms may not strongly affect smoking behavior but may possibly have an effect on nicotine dependence.
authors.affiliation: Department of Hygiene and Preventive Medicine, Fukushima Medical University School of Medicine.
authors.forename: Teruna
authors.initials: T
authors.lastname: Ito
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Tsuji
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Mori
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kanda
authors.forename: Tomoo
authors.initials: T
authors.lastname: Hidaka
authors.forename: Takeyasu
authors.initials: T
authors.lastname: Kakamu
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kumagai
authors.forename: Takehito
authors.initials: T
authors.lastname: Hayakawa
authors.forename: Yoneatsu
authors.initials: Y
authors.lastname: Osaki
authors.forename: And Tetsuhito
authors.initials: AT
authors.lastname: Fukushima
mesh_terms: D000328:Adult; D065637:Cytochrome P-450 CYP2A6 / Q000235:genetics*; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012907:Smoking; D014029:Tobacco Use Disorder / Q000235:genetics*; D055815:Young Adult
chemical_list: C585710:CYP2A6 protein, human; D065637:Cytochrome P-450 CYP2A6