title: Accelerated clearance of human red blood cells in a rat transfusion model.
abstract: BACKGROUND
  Animal models are valuable in transfusion research. Use of human red blood cells (RBCs) in animal models facilitates extrapolation of the impact of storage conditions to the human condition but may be hampered by the use of cross species.
  
  
  METHODS
  Investigation of clearance and posttransfusion recovery in a rat model using fresh and stored human RBCs.
  
  
  RESULTS
  Directly following transfusion, human RBCs could be detected in the circulation of all recipients, with higher recovery rates for stored RBCs than for fresh RBCs. After 24 h following transfusion, no donor RBCs could be detected in the circulation, but donor RBCs could be detected in all organs of all recipients.
  
  
  CONCLUSIONS
  The use of human donor RBCs in a rat transfusion model resulted in clearance from cells from the circulation. Donor cells were found in different organs of the recipients. Rat transfusion models are thus not appropriate to study the efficacy of human RBC transfusion.
authors.affiliation: Department of Intensive Care, Academic Medical Center, Meibergdreef 9, 1105AZ, Amsterdam, the Netherlands. m.straat@amc.uva.nl.|Laboratory of Experimental Intensive Care and Anesthesiology, Academic Medical Center, Meibergdreef 9, 1105AZ, Amsterdam, the Netherlands. m.straat@amc.uva.nl.
authors.forename: M
authors.initials: M
authors.lastname: Straat
authors.affiliation: Sanquin Department of Blood Cell Research, Plesmanlaan 125, Amsterdam, the Netherlands. t.klei@sanquin.nl.
authors.forename: Trl
authors.initials: T
authors.lastname: Klei
authors.affiliation: Sanquin Department of Blood Cell Research, Plesmanlaan 125, Amsterdam, the Netherlands. d.dekorte@sanquin.nl.
authors.forename: D
authors.initials: D
authors.lastname: de Korte
authors.affiliation: Sanquin Department of Blood Cell Research, Plesmanlaan 125, Amsterdam, the Netherlands. r.vanbruggen@sanquin.nl.
authors.forename: R
authors.initials: R
authors.lastname: van Bruggen
authors.affiliation: Department of Intensive Care, Academic Medical Center, Meibergdreef 9, 1105AZ, Amsterdam, the Netherlands. n.p.juffermans@amc.uva.nl.|Laboratory of Experimental Intensive Care and Anesthesiology, Academic Medical Center, Meibergdreef 9, 1105AZ, Amsterdam, the Netherlands. n.p.juffermans@amc.uva.nl.
authors.forename: N P
authors.initials: NP
authors.lastname: Juffermans
keywords: Red blood cell; Red blood cell recovery; Storage lesion; Transfusion model