title: A Novel Aldo-Keto Reductase (AKR17A1) of Anabaena sp. PCC 7120 Degrades the Rice Field Herbicide Butachlor and Confers Tolerance to Abiotic Stresses in E. coli.
abstract: Present study deals with the identification of a novel aldo/keto reductase, AKR17A1 from Anabaena sp. PCC7120 and adds on as 17th family of AKR superfamily drawn from a wide variety of organisms. AKR17A1 shares many characteristics of a typical AKR such as- (i) conferring tolerance to multiple stresses like heat, UV-B, and cadmium, (ii) excellent activity towards known AKR substrates (isatin and 2-nitrobenzaldehyde), and (iii) obligate dependence on NADPH as a cofactor for enzyme activity. The most novel attribute of AKR17A1, first reported in this study, is its capability to metabolize butachlor, a persistent rice field herbicide that adversely affects agro-ecosystem and non-target organisms. The AKR17A1 catalyzed- degradation of butachlor resulted into formation of 1,2-benzene dicarboxylic acid and 2,6 bis (1,1, dimethylethyl) 4,-methyl phenol as the major products confirmed by GC-MS analysis.
authors.affiliation: Molecular Biology Section, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi-221005, India.
authors.forename: Chhavi
authors.initials: C
authors.lastname: Agrawal
authors.affiliation: Molecular Biology Section, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi-221005, India.
authors.forename: Sonia
authors.initials: S
authors.lastname: Sen
authors.affiliation: Molecular Biology Section, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi-221005, India.
authors.forename: Shivam
authors.initials: S
authors.lastname: Yadav
authors.affiliation: Molecular Biology Section, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi-221005, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Rai
authors.affiliation: Molecular Biology Section, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi-221005, India.
authors.forename: Lal Chand
authors.initials: LC
authors.lastname: Rai
mesh_terms: D000083:Acetanilides / Q000378:metabolism* / Q000633:toxicity; D000449:Aldehyde Reductase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000074408:Aldo-Keto Reductases; D000595:Amino Acid Sequence; D017033:Anabaena / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology*; D001673:Biodegradation, Environmental; D002264:Carboxylic Acids / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D006540:Herbicides / Q000378:metabolism / Q000633:toxicity; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology*; D019800:Phenol / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects*; D013379:Substrate Specificity
chemical_list: D000083:Acetanilides; D002264:Carboxylic Acids; D006540:Herbicides; D019800:Phenol; C054409:butachlor; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase