title: Osteogenic Surface Modification Based on Functionalized Poly-P-Xylylene Coating.
abstract: The biotechnology to immobilize biomolecules on material surfaces has been developed vigorously due to its high potentials in medical applications. In this study, a simple and effective method was designed to immobilize biomolecules via amine-N-hydroxysuccinimide (NHS) ester conjugation reaction using functionalized poly-p-xylylene coating on material surfaces. The NHS ester functionalized coating is synthesized via chemical vapor deposition, a facile and solvent-less method, creating a surface which is ready to perform a one-step conjugation reaction. Bone morphogenetic protein 2 (BMP-2) is immobilized onto material surfaces by this coating method, forming an osteogenic environment. The immobilization process is controlled at a low temperature which does not damage proteins. This modified surface induces differentiation of preosteoblast into osteoblast, manifested by alkaline phosphatase (ALP) activity assay, Alizarin Red S (ARS) staining and the expression of osteogenic gene markers, Alpl and Bglap3. With this coating technology, immobilization of growth factors onto material surface can be achieved more simply and more effectively.
authors.affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Chih-Hao
authors.initials: CH
authors.lastname: Chang
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Shu-Yun
authors.initials: SY
authors.lastname: Yeh
authors.affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Bing-Heng
authors.initials: BH
authors.lastname: Lee
authors.affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Chia-Jie
authors.initials: CJ
authors.lastname: Chen
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Chiao-Tzu
authors.initials: CT
authors.lastname: Su
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Yen-Ting
authors.initials: YT
authors.lastname: Lin
authors.affiliation: Department of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan and School of Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Chien-Lin
authors.initials: CL
authors.lastname: Liu
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Hsien-Yeh
authors.initials: HY
authors.lastname: Chen
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000378:metabolism; D000818:Animals; D000880:Anthraquinones / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D011108:Polymers / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry; D013499:Surface Properties / Q000187:drug effects*
chemical_list: D000880:Anthraquinones; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D011108:Polymers; D013388:Succinimides; C004468:Alizarin Red S; C510878:ALPL protein, mouse; D000469:Alkaline Phosphatase; C001426:N-hydroxysuccinimide