title: Effect of multi-nutrient insufficiency on markers of one carbon metabolism in young women: response to a methionine load.
abstract: Multi-nutrient insufficiencies as a consequence of nutritional and economic factors are common in India and other developing countries. We have examined the impact of multi-nutrient insufficiency on markers of one carbon (1C) metabolism in the blood, and response to a methionine load in clinically healthy young women.
  
  
  
  Young women from Pune, India (n=10) and Cleveland, USA (n=13) were studied. Blood samples were obtained in the basal state and following an oral methionine load (50 mg/kg of body weight in orange juice). Plasma concentrations of vitamin B12, folate and B6 were measured in the basal state. The effect of methionine load on the levels of methionine, total homocysteine, cysteine, glutathione and amino acids was examined.
  
  
  
  Indian women were significantly shorter and lighter compared with the American women and had lower plasma concentration of vitamins B12, folate and B6, essential amino acids and glutathione, but higher concentration of total homocysteine. The homocysteine response to methionine load was higher in Indian women. The plasma concentrations of glycine and serine increased in the Indian women after methionine (in juice) load. A significant negative correlation between plasma B6 and homocysteine (r= -0.70), and plasma folate and glycine and serine levels were observed in the Indian group (P<0.05) but not in the American group.
  
  
  
  Multi-nutrient insufficiency in the Indian women caused unique changes in markers of whole body protein and 1C metabolism. These data would be useful in developing nutrient intervention strategies.
authors.affiliation: Kamalnayan Bajaj Diabetology Research Centre, King Edward Memorial Hospital Research Centre, Pune, India.
authors.forename: P
authors.initials: P
authors.lastname: Katre
authors.affiliation: Kamalnayan Bajaj Diabetology Research Centre, King Edward Memorial Hospital Research Centre, Pune, India.
authors.forename: S
authors.initials: S
authors.lastname: Joshi
authors.affiliation: Kamalnayan Bajaj Diabetology Research Centre, King Edward Memorial Hospital Research Centre, Pune, India.
authors.forename: D S
authors.initials: DS
authors.lastname: Bhat
authors.affiliation: Kamalnayan Bajaj Diabetology Research Centre, King Edward Memorial Hospital Research Centre, Pune, India.
authors.forename: M
authors.initials: M
authors.lastname: Deshmukh
authors.affiliation: Kamalnayan Bajaj Diabetology Research Centre, King Edward Memorial Hospital Research Centre, Pune, India.
authors.forename: N
authors.initials: N
authors.lastname: Gurav
authors.affiliation: Kamalnayan Bajaj Diabetology Research Centre, King Edward Memorial Hospital Research Centre, Pune, India.
authors.forename: S
authors.initials: S
authors.lastname: Pandit
authors.affiliation: Kamalnayan Bajaj Diabetology Research Centre, King Edward Memorial Hospital Research Centre, Pune, India.
authors.forename: H
authors.initials: H
authors.lastname: Lubree
authors.affiliation: Department of Pathobiology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA.
authors.forename: S
authors.initials: S
authors.lastname: Marczewski
authors.affiliation: Department of Pathobiology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA.
authors.forename: C
authors.initials: C
authors.lastname: Bennett
authors.affiliation: Department of Pathobiology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA.
authors.forename: L
authors.initials: L
authors.lastname: Gruca
authors.affiliation: Kamalnayan Bajaj Diabetology Research Centre, King Edward Memorial Hospital Research Centre, Pune, India.
authors.forename: K
authors.initials: K
authors.lastname: Kalyanaraman
authors.affiliation: Kamalnayan Bajaj Diabetology Research Centre, King Edward Memorial Hospital Research Centre, Pune, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Naik
authors.affiliation: Kamalnayan Bajaj Diabetology Research Centre, King Edward Memorial Hospital Research Centre, Pune, India.
authors.forename: C S
authors.initials: CS
authors.lastname: Yajnik
authors.affiliation: Department of Pathobiology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA.
authors.forename: S C
authors.initials: SC
authors.lastname: Kalhan
mesh_terms: D000328:Adult; D000596:Amino Acids / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001827:Body Height; D002244:Carbon / Q000378:metabolism; D005260:Female; D005492:Folic Acid / Q000097:blood; D005502:Food; D005978:Glutathione / Q000097:blood; D006710:Homocysteine / Q000097:blood; D006801:Humans; D007194:India; D044342:Malnutrition / Q000097:blood* / Q000503:physiopathology; D008715:Methionine / Q000008:administration & dosage* / Q000097:blood; D009820:Ohio; D014805:Vitamin B 12 / Q000097:blood; D014803:Vitamin B Complex / Q000097:blood
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D006710:Homocysteine; D014803:Vitamin B Complex; D002244:Carbon; D005492:Folic Acid; D008715:Methionine; D005978:Glutathione; D014805:Vitamin B 12