title: NOCA-1 functions with γ-tubulin and in parallel to Patronin to assemble non-centrosomal microtubule arrays in C. elegans.
abstract: Non-centrosomal microtubule arrays assemble in differentiated tissues to perform mechanical and transport-based functions. In this study, we identify Caenorhabditis elegans NOCA-1 as a protein with homology to vertebrate ninein. NOCA-1 contributes to the assembly of non-centrosomal microtubule arrays in multiple tissues. In the larval epidermis, NOCA-1 functions redundantly with the minus end protection factor Patronin/PTRN-1 to assemble a circumferential microtubule array essential for worm growth and morphogenesis. Controlled degradation of a γ-tubulin complex subunit in this tissue revealed that γ-tubulin acts with NOCA-1 in parallel to Patronin/PTRN-1. In the germline, NOCA-1 and γ-tubulin co-localize at the cell surface, and inhibiting either leads to a microtubule assembly defect. γ-tubulin targets independently of NOCA-1, but NOCA-1 targeting requires γ-tubulin when a non-essential putatively palmitoylated cysteine is mutated. These results show that NOCA-1 acts with γ-tubulin to assemble non-centrosomal arrays in multiple tissues and highlight functional overlap between the ninein and Patronin protein families.
authors.affiliation: Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, United States.|Biomedical Sciences Graduate Program, University of California, San Diego, La Jolla, United States.
authors.forename: Shaohe
authors.initials: S
authors.lastname: Wang
authors.affiliation: Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, United States.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: Institut Génétique Biologie Moléculaire Ceasllulaire, Faculté de médecine, Université de Strasbourg, Strasbourg, France.|Institut Clinique de la Souris, Illkirch-Graffenstaden, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Quintin
authors.affiliation: Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, United States.
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Green
authors.affiliation: Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, United States.
authors.forename: Dhanya K
authors.initials: DK
authors.lastname: Cheerambathur
authors.affiliation: Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, United States.
authors.forename: Stacy D
authors.initials: SD
authors.lastname: Ochoa
authors.affiliation: Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, United States.
authors.forename: Arshad
authors.initials: A
authors.lastname: Desai
authors.affiliation: Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, United States.
authors.forename: Karen
authors.initials: K
authors.lastname: Oegema
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D055503:Protein Multimerization; D014404:Tubulin / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D008869:Microtubule-Associated Proteins; C000608701:NOCA-1 protein, C elegans; D009687:Nuclear Proteins; C000599463:PTRN-1 protein, C elegans; D014404:Tubulin
keywords: C. elegans; NOCA-1; PTRN-1; Patronin; cell biology; developmental biology; ninein; non-centrosomal microtubule array; stem cells