title: Elevated permeability of the blood-brain barrier in mice intratracheally administered porcine pancreatic elastase.
abstract: Chronic obstructive pulmonary disease (COPD) shows progressive, irreversible airflow limitation induced by emphysema and lung inflammation. The aim of the present study was to determine if COPD conditions induce blood-brain barrier (BBB) dysfunction. We found that the intratracheal administration of porcine pancreatic elastase (PPE; 3 U) induced alveolar enlargement, increased neutrophil number in bronchoalveolar lavage fluid, and decreased blood oxygen saturation in mice at 21 days after inhalation. In parallel with these lung damages, BBB permeability to sodium fluorescein and Evans blue albumin was markedly increased. Our findings demonstrate that COPD conditions are associated with risk for BBB impairment.
authors.affiliation: Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan; BBB Laboratory, PharmaCo-Cell Co., Ltd., Nagasaki, Japan.
authors.forename: Fuyuko
authors.initials: F
authors.lastname: Takata
authors.affiliation: Department of Pharmaceutical and Health Care Management, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Tominaga
authors.affiliation: Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
authors.forename: Mitsuhisa
authors.initials: M
authors.lastname: Koga
authors.affiliation: Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Dohgu
authors.affiliation: Department of Pharmaceutical and Health Care Management, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
authors.forename: Koujiro
authors.initials: K
authors.lastname: Futagami
authors.affiliation: Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamauchi
authors.affiliation: Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan; BBB Laboratory, PharmaCo-Cell Co., Ltd., Nagasaki, Japan. Electronic address: ykataoka@fukuoka-u.ac.jp.
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Kataoka
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism* / Q000503:physiopathology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004195:Disease Models, Animal; D005070:Evans Blue / Q000378:metabolism; D019793:Fluorescein / Q000378:metabolism; D007958:Leukocyte Count; D051379:Mice; D009504:Neutrophils; D010100:Oxygen / Q000097:blood; D010196:Pancreatic Elastase / Q000008:administration & dosage / Q000009:adverse effects*; D010539:Permeability; D029424:Pulmonary Disease, Chronic Obstructive / Q000139:chemically induced* / Q000378:metabolism* / Q000503:physiopathology*; D013552:Swine
chemical_list: D005070:Evans Blue; D010196:Pancreatic Elastase; D010100:Oxygen; D019793:Fluorescein
keywords: Blood–brain barrier; Chronic obstructive pulmonary disease; Porcine pancreatic elastase