title: The use of human adipose-derived stem cells based cytotoxicity assay for acute toxicity test.
abstract: Human adipose-derived stem cells (ADSC) were evaluated as cell culture model for cytotoxicity assay and toxicity prediction by using the neutral red uptake assay (NRU). In this study, we compared ADSC and the murine cell line BALB/c 3T3 clone A31 to predict the toxicity of 12 reference substances as recommended by the Interagency Coordinating Committee on the Validation of Alternative Methods. We predicted the LD50 for RC-rat-only weight and RC-rat-only millimole regressions for both cell culture models. For RC rat-only weight regression, both cells had the same accordance (50%), while for RC rat-only millimole regression, the accordance was 50% for ADSC and 42% for 3T3s. Thus, ADSC have similar capability for GHS class prediction as the 3T3 cell line for the evaluated reference substances. Therefore, ADSCs showed the potential to be considered a novel model for use in evaluating cytotoxicity in drug development and industry as well as for regulatory purposes to reduce or replace the use of laboratory animals with acceptable sensitivity for toxicity prediction in humans. These cells can be used to complete the results from other models, mainly because of its human origin. Moreover, it is less expensive in comparison with other existing models.
authors.affiliation: Laboratório de Biologia Básica de Células-tronco, Instituto Carlos Chagas, Fiocruz, Curitiba, PR, Brazil.
authors.forename: Ana Paula Ressetti
authors.initials: AP
authors.lastname: Abud
authors.affiliation: Laboratório de Biologia Básica de Células-tronco, Instituto Carlos Chagas, Fiocruz, Curitiba, PR, Brazil.
authors.forename: Jaiesa
authors.initials: J
authors.lastname: Zych
authors.affiliation: Laboratório de Biologia Básica de Células-tronco, Instituto Carlos Chagas, Fiocruz, Curitiba, PR, Brazil.
authors.forename: Thamile Luciane
authors.initials: TL
authors.lastname: Reus
authors.affiliation: Laboratório de Biologia Básica de Células-tronco, Instituto Carlos Chagas, Fiocruz, Curitiba, PR, Brazil.
authors.forename: Crisciele
authors.initials: C
authors.lastname: Kuligovski
authors.affiliation: Laboratório de Biologia Básica de Células-tronco, Instituto Carlos Chagas, Fiocruz, Curitiba, PR, Brazil; Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: de Moraes
authors.affiliation: Laboratório de Biologia Básica de Células-tronco, Instituto Carlos Chagas, Fiocruz, Curitiba, PR, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Dallagiovanna
authors.affiliation: Laboratório de Biologia Básica de Células-tronco, Instituto Carlos Chagas, Fiocruz, Curitiba, PR, Brazil. Electronic address: alessandra_aguiar@fiocruz.br.
authors.forename: Alessandra Melo
authors.initials: AM
authors.lastname: de Aguiar
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D041702:BALB 3T3 Cells; D001681:Biological Assay; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020128:Inhibitory Concentration 50; D051379:Mice; D015203:Reproducibility of Results; D018570:Risk Assessment; D013234:Stem Cells / Q000187:drug effects* / Q000473:pathology; D023382:Toxicity Tests, Acute / Q000379:methods*
keywords: Cytotoxicity; Stem cells; Toxicity prediction