title: Short communication: Quantification of carbohydrates in whey permeate products using high-performance anion-exchange chromatography with pulsed amperometric detection.
abstract: A method was developed for the characterization and quantification of the disaccharide lactose and 3 major bovine milk oligosaccharides (BMO) in dairy streams. Based on high-performance anion-exchange chromatography-pulsed amperometric detection (HPAE-PAD), this method is advantageous because it requires minimal sample preparation and achieves good chromatographic separation of oligosaccharide isomers within 30min. The linear dynamic range and limit of detection were 0.1 to 10mg/L and 0.03 to 0.22mg/L, respectively. Mean recoveries of the BMO were excellent and ranged from 98.4 to 100.4%. Without complicated sample preparation procedures, this HPAE-PAD method measured BMO [3'-sialyllactose (3'SL), 6'-sialyllactose (6'SL), and 6'-sialyllactosamine (6'SLN)] and lactose using a single instrument, therefore increasing the accuracy of the measurement and applicability for the dairy industry. In colostrum whey permeate, 3'SL, 6'SL, and 6'SLN were 94, 29, and 46mg/L, respectively. This work is the first to demonstrate that some commercial products, currently marketed for supporting a healthy immune system, contain significant amounts of bioactive BMO and therefore, carry additional bioactivities.
authors.affiliation: Department of Food Science and Technology, University of California, Davis 95616; Foods for Health Institute, University of California, Davis 95616.
authors.forename: Hyeyoung
authors.initials: H
authors.lastname: Lee
authors.affiliation: Departamento de Tecnologia de Alimentos, Faculdade de Veterinaria, Universidade Federal Fluminense, Niteroi 24230-340, Rio de Janeiro, Brazil.
authors.forename: Vitor Luiz
authors.initials: VL
authors.lastname: de MeloSilva
authors.affiliation: Department of Food Science and Technology, University of California, Davis 95616.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Food Science and Technology, University of California, Davis 95616; Foods for Health Institute, University of California, Davis 95616. Electronic address: dbarile@ucdavis.edu.
authors.forename: Daniela
authors.initials: D
authors.lastname: Barile
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003126:Colostrum / Q000737:chemistry; D004040:Dietary Carbohydrates / Q000032:analysis*; D007785:Lactose / Q000031:analogs & derivatives / Q000032:analysis; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis; D015203:Reproducibility of Results; D000067796:Whey / Q000737:chemistry*
chemical_list: C421467:3'-sialyllactose; C403777:6'-sialyllactose; D004040:Dietary Carbohydrates; D009844:Oligosaccharides; C062923:N-acetylneuraminosyl(alpha2-6)lactosamine; D007785:Lactose
keywords: bovine milk oligosaccharide; high-performance anion-exchange chromatography-pulsed amperometric detection; lactose; whey permeate