title: Global translation variations in host cells upon attack of lytic and sublytic Staphylococcus aureus α-haemolysin.
abstract: Genome-wide analyses of translation can provide major contributions in our understanding of the complex interplay between virulent factors and host cells. So far, the activation of host translational control mechanisms by bacterial toxins, owing to specific recruitment of mRNAs, RNA-binding proteins (RBPs) and ncRNAs (non-coding RNAs), are far from being understood. In the present study, we characterize for the first time the changes experienced by the translational control system of host cells in response to the well-known Staphylococcus aureus α-haemolysin (AHL) under both sublytic and lytic conditions. By comparing variations occurring in the cellular transcriptome and translatome, we give evidence that global gene expression is primarily rewired at the translational level, with the contribution of the RBP ELAVL1 (HuR) in the sublytic response. These results reveal the importance of translational control during host-pathogen interaction, opening new approaches for AHL-induced diseases.
authors.affiliation: Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, via delle Regole, 101 38122 Mattarello, Trento, Italy CNR Institute of Biophysics & Bruno Kessler Foundation, via alla Cascata 56/C, 38123 Trento, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Clamer
authors.affiliation: Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, via delle Regole, 101 38122 Mattarello, Trento, Italy.
authors.forename: Toma
authors.initials: T
authors.lastname: Tebaldi
authors.affiliation: CNR Institute of Biophysics & Bruno Kessler Foundation, via alla Cascata 56/C, 38123 Trento, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Marchioretto
authors.affiliation: CNR Institute of Biophysics & Bruno Kessler Foundation, via alla Cascata 56/C, 38123 Trento, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Bernabò
authors.affiliation: Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, via delle Regole, 101 38122 Mattarello, Trento, Italy.
authors.forename: Efrem
authors.initials: E
authors.lastname: Bertini
authors.affiliation: Department of Physics, University of Trento, via Sommarive 14, 38123 Povo, Trento, Italy.
authors.forename: Graziano
authors.initials: G
authors.lastname: Guella
authors.affiliation: CNR Institute of Biophysics & Bruno Kessler Foundation, via alla Cascata 56/C, 38123 Trento, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Dalla Serra
authors.affiliation: Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, via delle Regole, 101 38122 Mattarello, Trento, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Quattrone
authors.affiliation: Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, via delle Regole, 101 38122 Mattarello, Trento, Italy CNR Institute of Biophysics & Bruno Kessler Foundation, via alla Cascata 56/C, 38123 Trento, Italy gabriella.viero@cnr.it.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Viero
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D000067780:ELAV-Like Protein 1 / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D014644:Genetic Variation / Q000187:drug effects*; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D015151:Immunoblotting; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D014176:Protein Biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome / Q000235:genetics*
chemical_list: D001427:Bacterial Toxins; D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; D006460:Hemolysin Proteins; C021091:staphylococcal alpha-toxin
keywords: HuR; RNA-binding protein; bacterial toxins; polysomal profiling; pore-forming toxins; transcriptome; translational control; translatome