title: microRNA-17-5p Modulates Bacille Calmette-Guerin Growth in RAW264.7 Cells by Targeting ULK1.
abstract: To explore the potential roles of miRNAs in controlling the survival of mycobacteria in macrophages, miR-17-5p in the regulation of Bacillus Calmette-Guérin(BCG)growth in the macrophage RAW264.7 cells was interrogated. Our results reveal that an infection of BCG shows a time-dependent up-regulation of miR-17-5p in RAW264.7 cells in early phase; importantly, excessive expression of miR-17-5p in these cells exhibits an increased propagation of intracellular BCG. Mechanistically, the Unc-51 like autophagy activating kinase 1 (ULK1), an initial molecular of autophagy are identified as novel target of miR-17-5p, the miR-17-5p is capable of targeting down-regulating the expression of ULK1 protein. In addition, an overexpression of miR-17-5p in RAW264.7 cells is correlated with repression of ULK1 and the autophagosome related proteins LC3I/II. These results imply that miR-17-5p may be able to arrest the maturation of mycobacterial phagosomes in part by targeting ULK1, subsequently reduces the ability of host cells to kill intracellular BCG.
authors.affiliation: School of LaboratoryMedicine, Ningxia Medical University, Yinchuan, 750004, China; General Hospital of Ningxia Medical University, Yinchuan, 750004, China; Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, Yinchuan, 750004, China.
authors.forename: Xiangguo
authors.initials: X
authors.lastname: Duan
authors.affiliation: School of LaboratoryMedicine, Ningxia Medical University, Yinchuan, 750004, China; Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, Yinchuan, 750004, China; Taian City Central Hospital,Taian City, Shandong Province, 271000, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: School of LaboratoryMedicine, Ningxia Medical University, Yinchuan, 750004, China; Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, Yinchuan, 750004, China.
authors.forename: Shuqin
authors.initials: S
authors.lastname: Ding
authors.affiliation: General Hospital of Ningxia Medical University, Yinchuan, 750004, China; Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, Yinchuan, 750004, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wei
authors.affiliation: School of LaboratoryMedicine, Ningxia Medical University, Yinchuan, 750004, China; Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, Yinchuan, 750004, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Su
authors.affiliation: School of LaboratoryMedicine, Ningxia Medical University, Yinchuan, 750004, China; Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, Yinchuan, 750004, China.
authors.forename: Hongpeng
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of LaboratoryMedicine, Ningxia Medical University, Yinchuan, 750004, China; General Hospital of Ningxia Medical University, Yinchuan, 750004, China; Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, Yinchuan, 750004, China.
authors.forename: Guangxian
authors.initials: G
authors.lastname: Xu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001343:Autophagy / Q000235:genetics; D000071189:Autophagy-Related Protein-1 Homolog; D015971:Gene Expression Regulation, Enzymologic; D057809:HEK293 Cells; D006801:Humans; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology*; D051379:Mice; D035683:MicroRNAs / Q000502:physiology*; D009163:Mycobacterium bovis / Q000254:growth & development* / Q000502:physiology; D010588:Phagosomes / Q000235:genetics / Q000382:microbiology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D000067996:RAW 264.7 Cells
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; C543614:Mirn17 microRNA, mouse; D000071189:Autophagy-Related Protein-1 Homolog; D017346:Protein Serine-Threonine Kinases; C112444:Ulk1 protein, mouse