title: Selective accumulation of the complement membrane attack complex in aging choriocapillaris.
abstract: The complement membrane attack complex (MAC) shows increased abundance in the choriocapillaris during normal aging and is especially prevalent in age-related macular degeneration (AMD). While perivascular MAC accumulation occurs in the choroid, it is not well understood whether similar deposition occurs in other aging tissues. In this study we examined the abundance of MAC across multiple human tissues. For studies on fixed tissues, paraffin sections were obtained from six human donor eyes and a commercially available tissue array containing 19 different tissues. Immunohistochemical labeling was performed using antibodies directed against the MAC and intercellular adhesion molecule-1 (ICAM-1), as well as the lectin Ulex europaeus agglutinin-I (UEA-I). The choriocapillaris was the only tissue with high levels of the MAC, which was not detected in any of the 38 additional samples from 19 tissues. ICAM-1 was abundantly expressed in the majority of tissues evaluated, and UEA-I labeled the vasculature in all tissues. A second experiment was performed using unfixed frozen sections of RPE-choroid and 7 extraocular tissues, which confirmed the relatively limited localization of the MAC to the choriocapillaris. In comparison to other tissues assessed, the restricted accumulation of MAC in the choriocapillaris may, in part, explain the specificity of AMD to the neural retina, RPE and choroid, and the relative absence of systemic pathology in this disease.
authors.affiliation: The Stephen A. Wynn Institute for Vision Research and the Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA, USA.
authors.forename: Kathleen R
authors.initials: KR
authors.lastname: Chirco
authors.affiliation: The Stephen A. Wynn Institute for Vision Research and the Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA, USA.
authors.forename: Budd A
authors.initials: BA
authors.lastname: Tucker
authors.affiliation: The Stephen A. Wynn Institute for Vision Research and the Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA, USA.
authors.forename: Edwin M
authors.initials: EM
authors.lastname: Stone
authors.affiliation: The Stephen A. Wynn Institute for Vision Research and the Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA, USA. Electronic address: Robert-Mullins@uiowa.edu.
authors.forename: Robert F
authors.initials: RF
authors.lastname: Mullins
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000473:pathology; D002196:Capillaries / Q000378:metabolism*; D002829:Choroid / Q000378:metabolism*; D015938:Complement Membrane Attack Complex / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008268:Macular Degeneration / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D037121:Plant Lectins / Q000378:metabolism
chemical_list: D015938:Complement Membrane Attack Complex; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D018799:Intercellular Adhesion Molecule-1
keywords: Age-related macular degeneration; Choriocapillaris; Choroid; Complement; Microvasculature