title: Monitoring of biogenic amines and drugs of various therapeutic groups in urine samples with use of HPLC.
abstract: A high-performance liquid chromatography method for simultaneous separation and determination of biogenic amines [dopamine, epinephrine, serotonin and its six metabolites (normetanephrine, metanephrine, 3,4-dihydroxyphenylacetic acid, 4-hydroxy-3-methoxyphenylglycol, homovanilic acid and 5-hydroxyindoloacetic acid)] with drugs from different therapeutically groups [analgesics (paracetamol, metamizol), diuretics (furosemide) and antibiotics (cefazolin, fluconazole)] was developed. A chromatographic column with pre-column with octadecylsilane phase (C18e ) and two detectors - diode array serial connected and fluorescence - was used. Gradient elution of mixture of acetate buffer (pH 4.66) and methanol as a mobile phase was applied. The limit of detection (LOD) of 8-10 ng/mL and limit of quantitation (LOQ) of 24-30 ng/mL for biogenic amines, as well as the LOD of 50-100 ng/mL and the LOQ of 150-300 ng/mL for drugs, were determined. The applied sample preparation method allowed recoveries of 93% for the biogenic amines and 92% for the drugs to be achieved. The developed procedure has been applied to simultaneous determination of the examined compounds in urine samples and could be used in clinical analysis.
authors.affiliation: Silesian University of Technology, Faculty of Chemistry, Department of Inorganic, Analytical Chemistry and Electrochemistry, Strzody 7 Str., 44-100, Gliwice, Poland.
authors.forename: Irena
authors.initials: I
authors.lastname: Baranowska
authors.affiliation: Silesian University of Technology, Faculty of Chemistry, Department of Inorganic, Analytical Chemistry and Electrochemistry, Strzody 7 Str., 44-100, Gliwice, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Płonka
mesh_terms: D001679:Biogenic Amines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D004364:Pharmaceutical Preparations / Q000652:urine*; D012821:Silanes / Q000737:chemistry
chemical_list: D001679:Biogenic Amines; D004364:Pharmaceutical Preparations; D012821:Silanes; C024779:octadecylsilane
keywords: HPLC; biogenic amines; drugs; urine