title: Application of biospeckles for assessment of structural and cellular changes in muscle tissue.
abstract: A modified spatial-temporal speckle correlation technique for operational assessment of structural changes in muscle tissues after slaughtering is considered. Coefficient of biological activity as a quantitative indicator of structural changes of biochemical processes in biological tissues is proposed. The experimental results have shown that this coefficient properly evaluates the biological activity of pig and chicken muscle tissue samples. Studying the degradation processes in muscle tissue during long-time storage in a refrigerator by measuring the spatial-temporal dynamics of biospeckle patterns is carried out. The reduction of the bioactivity level of refrigerated muscle tissue samples connected with the initiation of muscle fiber cracks and ruptures, reduction of sarcomeres, nuclei deformation, nuclear chromatin diminishing, and destruction of mitochondria is analyzed.
authors.affiliation: Karpenko Physico-Mechanical Institute of the National Academy of Sciences of Ukraine, Department of Optical-Digital Diagnostic Systems, 5 Naukova Street, Lviv 79060, Ukraine.
authors.forename: Oleksandr P
authors.initials: OP
authors.lastname: Maksymenko
authors.affiliation: Karpenko Physico-Mechanical Institute of the National Academy of Sciences of Ukraine, Department of Optical-Digital Diagnostic Systems, 5 Naukova Street, Lviv 79060, Ukraine.
authors.forename: Leonid I
authors.initials: LI
authors.lastname: Muravsky
authors.affiliation: State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, HPLC Laboratory, 11 Donetska Street, Lviv 79019, Ukraine.
authors.forename: Mykola I
authors.initials: MI
authors.lastname: Berezyuk
mesh_terms: D000818:Animals; D001329:Autolysis; D002645:Chickens; D061353:Food Storage; D008027:Light; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D061848:Optical Imaging; D012034:Refrigeration; D012542:Scattering, Radiation; D013552:Swine