title: Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose.
abstract: BACKGROUND
  Butane-2,3-diol (2,3-BD) is a fuel and platform biochemical with various industrial applications. 2,3-BD exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. Microbial fermentative processes have been reported for (2R,3R)-2,3-BD and meso-2,3-BD production.
  
  
  RESULTS
  The production of (2S,3S)-2,3-BD from glucose was acquired by whole cells of recombinant Escherichia coli coexpressing the α-acetolactate synthase and meso-butane-2,3-diol dehydrogenase of Enterobacter cloacae subsp. dissolvens strain SDM. An optimal biocatalyst for (2S,3S)-2,3-BD production, E. coli BL21 (pETDuet-PT7-budB-PT7-budC), was constructed and the bioconversion conditions were optimized. With the addition of 10 mM FeCl3 in the bioconversion system, (2S,3S)-2,3-BD at a concentration of 2.2 g/L was obtained with a stereoisomeric purity of 95.0 % using the metabolically engineered strain from glucose.
  
  
  CONCLUSIONS
  The engineered E. coli strain is the first one that can be used in the direct production of (2S,3S)-2,3-BD from glucose. The results demonstrated that the method developed here would be a promising process for efficient (2S,3S)-2,3-BD production.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 People's Republic of China.
authors.forename: Haipei
authors.initials: H
authors.lastname: Chu
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xin
authors.affiliation: Rizhao Entry-Exit Inspection and Quarantine Bureau, Rizhao, 276800 People's Republic of China.
authors.forename: Peihai
authors.initials: P
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 People's Republic of China.
authors.forename: Lixiang
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 People's Republic of China.
authors.forename: Xiuxiu
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 People's Republic of China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 People's Republic of China.
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
keywords: (2S,3S)-Butane-2,3-diol; Metabolic engineering; meso-Butane-2,3-diol dehydrogenase; α-Acetolactate synthase