title: Rotavirus disrupts cytoplasmic P bodies during infection.
abstract: Cytoplasmic Processing bodies (P bodies), the RNA-protein aggregation foci of translationally stalled and potentially decaying mRNA, have been reported to be differentially modulated by viruses. Rotavirus, the causative agent of acute infantile gastroenteritis is a double stranded RNA virus which completes its entire life-cycle exclusively in host cell cytoplasm. In this study, the fate of P bodies was investigated upon rotavirus infection. It was found that P bodies get disrupted during rotavirus infection. The disruption occurred by more than one different mechanism where deadenylating P body component Pan3 was degraded by rotavirus NSP1 and exonuclease XRN1 along with the decapping enzyme hDCP1a were relocalized from cytoplasm to nucleus. Overall the study highlights decay and subcellular relocalization of P body components as novel mechanisms by which rotavirus subverts cellular antiviral responses.
authors.affiliation: Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata 700010, West Bengal, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Bhowmick
authors.affiliation: Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata 700010, West Bengal, India.
authors.forename: Arpita
authors.initials: A
authors.lastname: Mukherjee
authors.affiliation: Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata 700010, West Bengal, India.
authors.forename: Upayan
authors.initials: U
authors.lastname: Patra
authors.affiliation: Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata 700010, West Bengal, India. Electronic address: chawlam70@gmail.com.
authors.forename: Mamta
authors.initials: M
authors.lastname: Chawla-Sarkar
mesh_terms: D000818:Animals; D002460:Cell Line; D003593:Cytoplasm / Q000821:virology*; D000882:Haplorhini; D054884:Host-Pathogen Interactions; D046911:Macromolecular Substances / Q000378:metabolism*; D009698:Nucleoproteins / Q000378:metabolism; D012401:Rotavirus / Q000502:physiology*; D014779:Virus Replication
chemical_list: D046911:Macromolecular Substances; D009698:Nucleoproteins
keywords: Degradation; Host–virus interaction; Nuclear translocation; P-Body; Rotavirus