title: Colorimetric and fluorescence detection of G-quadruplex nucleic acids with a coumarin-benzothiazole probe.
abstract: A colorimetric and red-emitting fluorescent dual probe for G-quadruplexes was devised with a conjugated coumarin-benzothiazole scaffold. Its significant and distinct changes in both color and fluorescence enable the label-free and visual detection of G-quadruplex structures. In addition, this probe gives a distinct strong emission response to the nucleoli in fixed cells imaging, which might be attributed to the interaction between the probe and rDNA G-quadruplex based on the chromatin immunoprecipitation assay. All these results suggest its promising application prospects in the G-quadruplex research field.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, P. R. China.
authors.forename: Jin-wu
authors.initials: JW
authors.lastname: Yan
authors.forename: Yi-guang
authors.initials: YG
authors.lastname: Tian
authors.forename: Jia-heng
authors.initials: JH
authors.lastname: Tan
authors.forename: Zhi-shu
authors.initials: ZS
authors.lastname: Huang
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D002466:Cell Nucleolus / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D003124:Colorimetry / Q000379:methods*; D003374:Coumarins / Q000737:chemistry*; D004275:DNA, Ribosomal / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D020349:Surface Plasmon Resonance
chemical_list: D052160:Benzothiazoles; D003374:Coumarins; D004275:DNA, Ribosomal; D005456:Fluorescent Dyes; C104515:benzothiazole coumarin; C030123:coumarin