title: PIWIL2 promotes progression of non-small cell lung cancer by inducing CDK2 and Cyclin A expression.
abstract: BACKGROUND
  PIWI proteins have important roles in tumorigenesis due to their interaction with piRNAs. Recent studies suggest that PIWI proteins affect prognosis of various cancers.
  
  
  METHODS
  In the present study, PIWI genes expression was assayed in non-small cell lung cancer (NSCLC). To determine the effects of PIWIL2 on NSCLC cells, overexpression and interference assays were performed using the A549 and H460 cell lines. The tumor formation model was performed to demonstrate the effects of PIWIL2 on tumor formation in vivo.
  
  
  RESULTS
  PIWIL2 was increased both at the RNA and protein level in malignant cancer tissues compared with adjacent normal tissue. Moreover, increased PIWIL2 gene expression was negatively correlated with prognosis in NSCLC patients. Overexpression and interference of PIWIL2 promoted and depressed cell proliferation, respectively. Meanwhile, PIWIL2 interference arrested cells at the G2/M stage. In addition, we found that CDK2 and Cyclin A expression were correlated with PIWIL2 expression. Moreover, transfection of PIWIL2 promoted tumor growth in nude mice.
  
  
  CONCLUSIONS
  Our findings shed light on the function of PIWIL2 in NSCLC and suggest potential prognostic and therapeutic value.
authors.affiliation: The First Affiliated Hospital, China Medical University, NO. 155, Nanjing North Street, Heping District, Shenyang, 110001, Liaoning, China. han_seal@163.com.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Qu
authors.affiliation: The Forth Affiliated Hospital, China Medical University, Shenyang, China. lnljl520@126.com.
authors.forename: Jinlu
authors.initials: J
authors.lastname: Liu
authors.affiliation: The First Affiliated Hospital, China Medical University, NO. 155, Nanjing North Street, Heping District, Shenyang, 110001, Liaoning, China. xwzhong@mail.cmu.edu.cn.
authors.forename: Xinwen
authors.initials: X
authors.lastname: Zhong
authors.affiliation: The First Affiliated Hospital, China Medical University, NO. 155, Nanjing North Street, Heping District, Shenyang, 110001, Liaoning, China. 13889853625@163.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital, China Medical University, NO. 155, Nanjing North Street, Heping District, Shenyang, 110001, Liaoning, China. 18040097645@163.com.
authors.forename: Qigang
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000818:Animals; D060565:Argonaute Proteins / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019925:Cyclin A / Q000378:metabolism*; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D011379:Prognosis; D012313:RNA / Q000378:metabolism; D014162:Transfection; D016896:Treatment Outcome
chemical_list: D060565:Argonaute Proteins; D019925:Cyclin A; C486868:PIWIL2 protein, human; C448521:Piwil2 protein, mouse; D012313:RNA; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2