title: Rapamycin inhibits the proliferation of SW1990 pancreatic cancer cell.
abstract: OBJECTIVE
  To study the effect of rapamycin on pancreatic cancer cell proliferation, we designed a serial of experiments in human pancreatic cancer cell line SW1990.
  
  
  METHODS
  SW1990 cells were treated with different concentrations of rapamycin. Cell proliferation was measured by CCK-8 assay and cell colony formation. Cell cycle and apoptosis was analyzed by flow cytometry. The existence of mTOR signaling pathway was demonstrated by immunocytochemistry. Western-blot and real time-PCR were used to test whether mTOR-signaling pathway was inhibited with rapamycin treatment.
  
  
  RESULTS
  Our results showed that rapamycin inhibited the cell colony formation and proliferation (p < 0.05). Rapamycin induced G1 cell cycle arrest (p < 0.05) but not cell apoptosis (p > 0.05). p-mTOR, p-p70S6K and p-4E-BP1 were expressed in the cytoplasm of SW1990 cells and those proteins were significantly reduced with rapamycin (p < 0.05).
  
  
  CONCLUSIONS
  Rapamycin inhibits SW1990 pancreatic cancer cell proliferation through inhibiting the activation of mTOR pathway.
authors.affiliation: Department of Gastroenterology, Shanghai First People's Hospital, Shanghai JiaoTong University, Shanghai, China. xumin73@126.com.
authors.forename: M
authors.initials: M
authors.lastname: Xu
authors.forename: L-M
authors.initials: LM
authors.lastname: Bu
authors.forename: K
authors.initials: K
authors.lastname: Wu
authors.forename: L-G
authors.initials: LG
authors.lastname: Lu
authors.forename: X-P
authors.initials: XP
authors.lastname: Wang
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D015398:Signal Transduction / Q000187:drug effects; D020123:Sirolimus / Q000494:pharmacology*
chemical_list: D000903:Antibiotics, Antineoplastic; D020123:Sirolimus