title: Aldehyde Dehydragenase 1 and Nodal as Significant Prognostic Markers in Colorectal Cancer.
abstract: This study aimed to analyze prognostic significance of aldehyde dehydragenase 1 (ALDH1) and Nodal expression in patients with colorectal cancer. ALDH1 and Nodal expressions were observed based on the immunohistochemistry staining from 108 colorectal cancer patients. Scores were given to the staining intensity and percentage of positive cells, and sum of two scores for each case was used to define the groups of ALDH1 and Nodal. We also investigated the protein and mRNA levels of ALDH1 and Nodal by Western blot and qRT-PCR assays. The results were analyzed with the clinicopathologic parameters of these patients. The results indicated that expressions of ALDH1 and Nodal were significantly correlated with the differentiation degree, metastasis, number of tumor positive lymph nodes and AJCC stage. ALDH1 was inclined to express more in the worse differentiated degrees, lymph node metastasis, and worse AJCC stage of colorectal cancer patients. And the expression of Nodal was inversely compared with ALDH1.While the expression of ALDH1 was inversely correlated with the Nodal (r = -0.709, P < 0.01).
authors.affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Harbin Medical University, Xuefu Road 246, Nangang District, Harbin, 150001, Heilongjiang, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Pathology, Harbin Medical University, Xuefu Road 157, Harbin, 150081, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Harbin Medical University, Xuefu Road 246, Nangang District, Harbin, 150001, Heilongjiang, People's Republic of China.
authors.forename: Fenghua
authors.initials: F
authors.lastname: Pei
authors.affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Harbin Medical University, Xuefu Road 246, Nangang District, Harbin, 150001, Heilongjiang, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Infectious Disease, The Second Affiliated Hospital of Harbin Medical University, Xuefu Road 246, Harbin, Heilongjiang, 150001, People's Republic of China.
authors.forename: Bingzhu
authors.initials: B
authors.lastname: Yan
authors.affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Harbin Medical University, Xuefu Road 246, Nangang District, Harbin, 150001, Heilongjiang, People's Republic of China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Geng
authors.affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Harbin Medical University, Xuefu Road 246, Nangang District, Harbin, 150001, Heilongjiang, People's Republic of China. bingrongliu1963@126.com.
authors.forename: Bingrong
authors.initials: B
authors.lastname: Liu
mesh_terms: D000368:Aged; D000080924:Aldehyde Dehydrogenase 1 Family; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D055457:Nodal Protein / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D050697:Retinal Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D007527:Isoenzymes; C526007:NODAL protein, human; D055457:Nodal Protein; D012333:RNA, Messenger; D000080924:Aldehyde Dehydrogenase 1 Family; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase
keywords: ALDH1; Colorectal cancer; Nodal; Predictive factors