title: Sugarcane Elongin C is involved in infection by sugarcane mosaic disease pathogens.
abstract: Sugarcane (Saccharum sp. hybrid) provides the main source of sugar for humans. Sugarcane mosaic disease (SMD) is a major threat to sugarcane production. Currently, control of SMD is mainly dependent on breeding resistant cultivars through hybridization, which is time-consuming. Understanding the mechanism of viral infection may facilitate novel strategies to breed cultivars resistant to SMD and to control the disease. In this study, a wide interaction was detected between the viral VPg protein and host proteins. Several genes were screened from sugarcane cDNA library that could interact with Sugarcane streak mosaic virus VPg, including SceIF4E1 and ScELC. ScELC was predicted to be a cytoplasmic protein, but subcellular localization analysis showed it was distributed both in cytoplasmic and nuclear, and interactions were also detected between ScELC and VPg of SCMV or SrMV that reveal ScELC was widely used in the SMD pathogen infection process. ScELC and VPgs interacted in the nucleus, and may function to enhance the viral transcription rate. ScELC also interacted with SceIF4E2 both in the cytoplasm and nucleus, but not with SceIF4E1 and SceIF4E3. These results suggest that ScELC may be essential for the function of SceIF4E2, an isomer of eIF4E.
authors.affiliation: Key Laboratory of Sugarcane Biology and Genetic Breeding, MOA, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, Fujian 350002, PR China.
authors.forename: Yushan
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: Key Laboratory of Sugarcane Biology and Genetic Breeding, MOA, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, Fujian 350002, PR China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Key Laboratory of Sugarcane Biology and Genetic Breeding, MOA, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, Fujian 350002, PR China.
authors.forename: Guangyuan
authors.initials: G
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Sugarcane Biology and Genetic Breeding, MOA, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, Fujian 350002, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Peng
authors.affiliation: Key Laboratory of Sugarcane Biology and Genetic Breeding, MOA, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, Fujian 350002, PR China.
authors.forename: Yanru
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Sugarcane Biology and Genetic Breeding, MOA, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, Fujian 350002, PR China. Electronic address: yyq287346@163.com.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Sugarcane Biology and Genetic Breeding, MOA, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, Fujian 350002, PR China. Electronic address: xujingsheng@126.com.
authors.forename: Jingsheng
authors.initials: J
authors.lastname: Xu
mesh_terms: D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D000075065:Elongin; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism; D015723:Gene Library; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D010940:Plant Proteins / Q000502:physiology*; D010957:Plasmids / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D031786:Saccharum / Q000502:physiology / Q000821:virology*; D014026:Nicotiana / Q000821:virology; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; C000616395:ELOC protein, human; D000075065:Elongin; D039561:Eukaryotic Initiation Factor-4E; D010940:Plant Proteins; D012367:RNA, Viral; D014157:Transcription Factors; D014764:Viral Proteins
keywords: BiFC; ScELC; SceIF4E; Sugarcane mosaic disease; VPg; Y2HS