title: Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles.
abstract: BACKGROUND
  Cbf11 and Cbf12, the fission yeast CSL transcription factors, have been implicated in the regulation of cell-cycle progression, but no specific roles have been described and their target genes have been only partially mapped.
  
  
  RESULTS
  Using a combination of transcriptome profiling under various conditions and genome-wide analysis of CSL-DNA interactions, we identify genes regulated directly and indirectly by CSL proteins in fission yeast. We show that the expression of stress-response genes and genes that are expressed periodically during the cell cycle is deregulated upon genetic manipulation of cbf11 and/or cbf12. Accordingly, the coordination of mitosis and cytokinesis is perturbed in cells with genetically manipulated CSL protein levels, together with other specific defects in cell-cycle progression. Cbf11 activity is nutrient-dependent and Δcbf11-associated defects are mitigated by inactivation of the protein kinase A (Pka1) and stress-activated MAP kinase (Sty1p38) pathways. Furthermore, Cbf11 directly regulates a set of lipid metabolism genes and Δcbf11 cells feature a stark decrease in the number of storage lipid droplets.
  
  
  CONCLUSIONS
  Our results provide a framework for a more detailed understanding of the role of CSL proteins in the regulation of cell-cycle progression in fission yeast.
authors.affiliation: Research Department of Genetics, Evolution & Environment and UCL Cancer Institute, University College London, London, United Kingdom; Department of Cell Biology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Převorovský
authors.affiliation: Department of Cell Biology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Oravcová
authors.affiliation: Department of Cell Biology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
authors.forename: Jarmila
authors.initials: J
authors.lastname: Tvarůžková
authors.affiliation: Department of Cell Biology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
authors.forename: Róbert
authors.initials: R
authors.lastname: Zach
authors.affiliation: Department of Cell Biology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Folk
authors.affiliation: Department of Cell Biology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
authors.forename: František
authors.initials: F
authors.lastname: Půta
authors.affiliation: Research Department of Genetics, Evolution & Environment and UCL Cancer Institute, University College London, London, United Kingdom.
authors.forename: Jürg
authors.initials: J
authors.lastname: Bähler
mesh_terms: D017868:Cyclic AMP-Dependent Protein Kinases / Q000235:genetics; D048749:Cytokinesis; D020869:Gene Expression Profiling / Q000379:methods; D015966:Gene Expression Regulation, Fungal; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D008938:Mitosis; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C569834:Cbf11 protein, S pombe; C569835:Cbf12 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; D017868:Cyclic AMP-Dependent Protein Kinases; D020928:Mitogen-Activated Protein Kinases; C095409:sty1 protein, S pombe