title: Phylogenetic analysis of human group C rotavirus in hospitalized children with gastroenteritis in Belém, Brazil.
abstract: Group C rotavirus (RVC) is potentially an important pathogen associated with acute gastroenteritis (AG), especially in outbreaks. This study aims to detect and molecularly characterize RVC in hospitalized children with AG in Belém, Brazil. From May 2008 to April 2011, 279 stools were subjected to reverse-transcription polymerase chain reaction targeting VP7, VP6, VP4, and NSP4 genes. RVC positivity rate was 2.1% (6/279) and phylogenetic analysis of positive samples yields genotype G4-P[2]-I2-E2. No evidence of zoonotic transmission and VP7 gene demonstrated close relationship with Asian strains. RVC surveillance is worth to expand information on evolutionary and epidemiological features of this virus.
authors.affiliation: Tropical Medicine Center, Federal University of Pará, Belém, Pará
authors.forename: Patrícia dos Santos
authors.initials: Pdos S
authors.lastname: Lobo
authors.affiliation: Tropical Medicine Center, Federal University of Pará, Belém, Pará
authors.forename: Sylvia de Fátima dos Santos
authors.initials: Sde F
authors.lastname: Guerra
authors.affiliation: Virology Section, Evandro Chagas Institute, Ananindeua, Pará
authors.forename: Jones Anderson Monteiro
authors.initials: JA
authors.lastname: Siqueira
authors.affiliation: Virology Section, Evandro Chagas Institute, Ananindeua, Pará
authors.forename: Luana da Silva
authors.initials: Lda S
authors.lastname: Soares
authors.affiliation: Virology Section, Evandro Chagas Institute, Ananindeua, Pará
authors.forename: Yvone Benchimol
authors.initials: YB
authors.lastname: Gabbay
authors.affiliation: Virology Section, Evandro Chagas Institute, Ananindeua, Pará
authors.forename: Alexandre C
authors.initials: AC
authors.lastname: Linhares
authors.affiliation: Virology Section, Evandro Chagas Institute, Ananindeua, Pará
authors.forename: Joana D'Arc Pereira
authors.initials: JD
authors.lastname: Mascarenhas
mesh_terms: D001938:Brazil / Q000453:epidemiology; D002673:Child, Hospitalized; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D017720:Molecular Epidemiology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D017361:Viral Nonstructural Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins
keywords: acute gastroenteritis; group C rotavirus; hospitalized children