title: The effects of tacrolimus on the activity and expression of tissue factor in the rat ovary with ischemia-reperfusion induced injury.
abstract: In the present study, the effects of tacrolimus on the activity and expression of tissue factor (TF) were investigated in the ovarian ischemia-reperfusion induced injury in rats. Twenty-eight female rats (8-12 weeks, 300-350 g) were divided into four groups: control, ischemia-reperfusion (IR), tacrolimus treated before ischemia (TBI), and tacrolimus treated before reperfusion (TBR) groups (n=7/per group). TF activity was measured using Quick's method, whereas TF expression was examined immunohistochemically. TF activity was significantly higher in all treated groups compared with the control group. Strong ovarian TF expression was demonstrated in the IR and TBR groups. Moreover, tacrolimus decreased TF activity in the TBI group compared with the IR group. The decreased activity of TF in the ovarian IR model may prevent IR-related inflammation during transplant procedure.
authors.affiliation: Department of Biochemistry, Faculty of Dentistry, Marmara University, Nisantasi, Istanbul, Turkey.
authors.forename: Unsal Veli
authors.initials: UV
authors.lastname: Ustundag
authors.affiliation: Zeynep Kamil Gynecologic and Pediatric Training and Research Hospital, Istanbul, Turkey.
authors.forename: Sadık
authors.initials: S
authors.lastname: Sahin
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Marmara University, Istanbul, Turkey.
authors.forename: Koray
authors.initials: K
authors.lastname: Ak
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Istanbul Medipol University, Istanbul, Turkey.
authors.forename: Ilknur
authors.initials: I
authors.lastname: Keskin
authors.affiliation: Department of Biochemistry, Faculty of Dentistry, Marmara University, Nisantasi, Istanbul, Turkey. Electronic address: ebruemekli@yahoo.com.
authors.forename: Ebru
authors.initials: E
authors.lastname: Emekli-Alturfan
mesh_terms: D000818:Animals; D005260:Female; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D010053:Ovary / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D016559:Tacrolimus / Q000494:pharmacology*; D013925:Thromboplastin / Q000378:metabolism*
chemical_list: D007166:Immunosuppressive Agents; D013925:Thromboplastin; D016559:Tacrolimus
keywords: Ischemia reperfusion; Ovary; Tacrolimus; Tissue factor