title: Description of Acinetobacter populi sp. nov. isolated from symptomatic bark of Populus x euramericana canker.
abstract: Five Gram-negative, non-motile, rod-shaped bacterial strains were isolated from cankers of Populus x euramericana collected from different locations in Puyang city, Henan Province, China. The five strains were characterized by nutritional and physiological testing and DNA sequence analysis. Haemolysis was not observed on agar media supplemented with sheep erythrocytes. The strains could be distinguished from members of most species of the genus Acinetobacter by their inability to assimilate L-arginine and benzoate. The five strains formed a single branch in phylogenetic trees based on 16S rRNA, gyrB and rpoB individual gene sequence analysis,indicating that they all belonged to a single taxon within the genus Acinetobacter. DNA-DNA hybridization results indicated that the five isolates represented to a single species that was separate from Acinetobacter puyangensis. On the basis of the phenotypic, genotypic and phylogenetic characteristics, the five strains are considered to represent a novel species of the genus Acinetobacter, for which the name Acinetobacter populi sp. nov. is proposed. The typestrain of A. populi sp. nov. is PBJ7T (CFCC 11170T=KCTC 42272T).
authors.affiliation: The Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing 100083, PR China.|The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Puyang Academy of Forestry, Puyang 457000, PR China.
authors.forename: Jupu
authors.initials: J
authors.lastname: Chang
authors.affiliation: Puyang Academy of Forestry, Puyang 457000, PR China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Guo
authors.affiliation: Heze Forest Protection Station, Heze 274002, PR China.
authors.forename: Hai-Ming
authors.initials: HM
authors.lastname: Wang
authors.affiliation: Puyang Academy of Forestry, Puyang 457000, PR China.
authors.forename: Shou-Jiang
authors.initials: SJ
authors.lastname: Xie
authors.affiliation: The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
authors.forename: Chun-Gen
authors.initials: CG
authors.lastname: Piao
authors.affiliation: The Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
mesh_terms: D000150:Acinetobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D024301:Plant Bark / Q000382:microbiology*; D032107:Populus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S