title: Micro 3D cell culture systems for cellular behavior studies: Culture matrices, devices, substrates, and in-situ sensing methods.
abstract: Microfabricated systems equipped with 3D cell culture devices and in-situ cellular biosensing tools can be a powerful bionanotechnology platform to investigate a variety of biomedical applications. Various construction substrates such as plastics, glass, and paper are used for microstructures. When selecting a construction substrate, a key consideration is a porous microenvironment that allows for spheroid growth and mimics the extracellular matrix (ECM) of cell aggregates. Various bio-functionalized hydrogels are ideal candidates that mimic the natural ECM for 3D cell culture. When selecting an optimal and appropriate microfabrication method, both the intended use of the system and the characteristics and restrictions of the target cells should be carefully considered. For highly sensitive and near-cell surface detection of excreted cellular compounds, SERS-based microsystems capable of dual modal imaging have the potential to be powerful tools; however, the development of optical reporters and nanoprobes remains a key challenge. We expect that the microsystems capable of both 3D cell culture and cellular response monitoring would serve as excellent tools to provide fundamental cellular behavior information for various biomedical applications such as metastasis, wound healing, high throughput screening, tissue engineering, regenerative medicine, and drug discovery and development.
authors.affiliation: Department of Bionanotechnology, Graduate School, Hanyang University - ERICA, Ansan, Korea.
authors.forename: Jonghoon
authors.initials: J
authors.lastname: Choi
authors.affiliation: Department of Bionanotechnology, Graduate School, Hanyang University - ERICA, Ansan, Korea.
authors.forename: Eun Kyu
authors.initials: EK
authors.lastname: Lee
authors.affiliation: Department of Bionanotechnology, Graduate School, Hanyang University - ERICA, Ansan, Korea.
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
authors.affiliation: New Technology Department, Corporate Technology Division, POSCO, Seoul, Korea.
authors.forename: Junhan
authors.initials: J
authors.lastname: Yuh
authors.affiliation: Department of Bionanotechnology, Graduate School, Hanyang University - ERICA, Ansan, Korea. wh@hanyang.ac.kr.
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Hong
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D001709:Biotechnology; D018929:Cell Culture Techniques / Q000295:instrumentation*; D045744:Cell Line, Tumor; D006801:Humans; D020100:Hydrogels; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*
chemical_list: D020100:Hydrogels
keywords: 3D cell culture microsystem; Bionanotechnology; Cellular biosensing; Functionalized hydrogels; Spheroid cell culture