title: Enhanced Cellular Uptake of Silica-Coated Magnetite Nanoparticles Compared with PEG-Coated Ones in Stem Cells.
abstract: Monodispersed magnetite (Fe3O4) nanoparticles (NPs) were prepared through the thermal decomposition method. The obtained NPs were surface modified with silica (SiO2) and polyethylene glycol (PEG), to enhance their stability in aqueous environment and their cellular uptake efficiency for biomedical applications. The NPs were characterized by X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), Fourier transform infrared (FT-IR) spectroscopy, and dynamic light scattering (DLS). The cytotoxicity of these NPs on bone marrow mesenchymal stem cells (BM-MSCs) was measured by MTT assay (cell viability test) at various concentrations (2, 5, 12.5, 25, and 50 µg/mL). The cells remained more than 90% viable at concentrations as high as 50 µg/mL. To compare the cellular uptake efficiency, these NPs were treated in BM-MSCs and the Fe concentration within the cells was measured by inductively coupled plasma-atomic emission spectrometry (ICP-AES) analysis. The uptake process displayed a time- and dose-dependency. The uptake amount of SiO2-coated Fe3O4 (Fe3O4@SiO2) NPs was about 10 times higher than that of the PEG-coated ones (Fe3O4@PEG).
authors.forename: Dong Heon
authors.initials: DH
authors.lastname: Lee
authors.forename: Myunggoo
authors.initials: M
authors.lastname: Kang
authors.forename: Hong Jai
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jeong Ah
authors.initials: JA
authors.lastname: Kim
authors.forename: Yun-Kyong
authors.initials: YK
authors.lastname: Choi
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Cho
authors.forename: Jung-Keug
authors.initials: JK
authors.lastname: Park
authors.forename: Tai Hyun
authors.initials: TH
authors.lastname: Park
authors.forename: Hyun
authors.initials: H
authors.lastname: Jung
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D008422:Materials Testing; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000633:toxicity; D012822:Silicon Dioxide / Q000737:chemistry* / Q000633:toxicity; D013234:Stem Cells / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology*; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D058185:Magnetite Nanoparticles; D011092:Polyethylene Glycols; D012822:Silicon Dioxide