title: Effects of Electroacupuncture on Chronic Unpredictable Mild Stress Rats Depression-Like Behavior and Expression of p-ERK/ERK and p-P38/P38.
abstract: We investigate the antidepressant-like effect and mechanism of electroacupuncture (EA) on a chronic unpredictable mild stress rats depression-like behavior. In our study, depression in rats was induced by unpredictable chronic mild stress (UCMS) and isolation for four weeks. Male Sprague-Dawley rats were randomly divided into four groups: Normal, Model, EA, and Sham EA. EA treatment was administered for two weeks, once a day for five days a week. Two acupoints, Yintang (EX-HN3) and Baihui (GV20), were selected. For sham EA, acupuncture needles were inserted shallowly into the acupoints: EX-HN3 and GV20. No electrostimulator was connected. The antidepressant-like effect of the electroacupuncture treatment was measured by sucrose intake test, open field test, and forced swimming test in rats. The protein levels of phosphorylated extracellular regulated protein kinases (p-ERK1/2)/ERK1/2 and p-P38/P38 in the hippocampus (HP) were examined by Western blot analysis. Our data demonstrate that EA treatment decreased the immobility time of forced swimming test and improved the sucrose solution intake in comparison to unpredictable chronic mild stress and placebo sham control. Electroacupuncture may act on depression by enhancing p-ERK1/2 and p-p38 in the hippocampus.
authors.affiliation: Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.affiliation: Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: She
authors.affiliation: Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Su
authors.affiliation: University of Maryland School of Medicine, Baltimore, MD 21201, USA.
authors.forename: Ruixin
authors.initials: R
authors.lastname: Zhang
authors.affiliation: University of Maryland School of Medicine, Baltimore, MD 21201, USA ; School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Lixing
authors.initials: L
authors.lastname: Lao
authors.affiliation: Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China.
authors.forename: Shifen
authors.initials: S
authors.lastname: Xu