title: Comparative Two-Dimensional Gel Electrophoresis of Trypanosoma cruzi Mammalian-Stage Forms in an Alkaline pH Range.
abstract: It is estimated that several million people are currently infected worldwide by the protozoan parasite, Trypanosoma cruzi, which causes Chagas disease. After mammalian host infection, a fundamental event is the differentiation from infective trypomastigotes into replicative amastigotes (amastigogenesis) inside host-cells. To unravel the particularities of both forms, it is essential to identify molecules presented in each form. Since T. cruzi gene expression regulation occurs mainly at posttranscriptional level, a proteomic approach is appropriate. Due to intrinsic difficulties with performing 2-DE in the alkaline pH range, there are no reports on 2-DE-based comparative proteome analysis of T. cruzi mammalianstage forms that focus on alkaline polypeptides. Here, we performed a comparative proteome analysis between tissue culture- derived trypomastigotes and extracellular amastigote-like cells using conditions optimized for the 6-11 pH range followed by identification by MALDI-TOF/TOF technology. The alkaline 2-DE maps from both forms show that proteins with a pI above 7.0 were not underrepresented (= 65% of proteins detected). Moreover the differences in protein expression between the Human-hosted T. cruzi forms corroborated previous proteomic studies and corresponded to their biological traits.
authors.forename: Adriana D
authors.initials: AD
authors.lastname: Magalhães
authors.forename: Rayner Myr L
authors.initials: RM
authors.lastname: Queiroz
authors.forename: Izabela M D
authors.initials: IM
authors.lastname: Bastos
authors.forename: Jaime M
authors.initials: JM
authors.lastname: Santana
authors.forename: Marcelo V
authors.initials: MV
authors.lastname: Sousa
authors.forename: Carlos André O
authors.initials: CA
authors.lastname: Ricart
authors.affiliation: Laboratory of Biochemistry and Protein Chemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia-DF, 70910-900, Brazil. charneau@unb.br.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Charneau
mesh_terms: D014355:Chagas Disease / Q000469:parasitology; D015180:Electrophoresis, Gel, Two-Dimensional; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006790:Host-Parasite Interactions; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D015800:Protozoan Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D020543:Proteome; D015800:Protozoan Proteins