title: The correlation between HLA-DRB1 and HLA-DQB1 gene polymorphisms and cytokines in HPV16 infected women with advanced cervical cancer.
abstract: OBJECTIVE
  To analyze the distribution of HLA-DRB1 and HLA-DQB1 alleles and its correlation with IFN-γ, IL-2, IL-6, IL-10 in HPV16 infected women with advanced cervical carcinoma.
  
  
  METHODS
  We collected 137 blood samples of cervical carcinoma patients diagnosed by pathology as cervical cancer in stage IIb-IVb before the treatment, and we gathered 175 blood samples of healthy women living in the local. We determined the genetic subtypes of HLA-DRB1 and HLA-DQB1, and we measured the concentration of IFN-γ, IL-2, IL-6 and IL-10. We compared the difference of cytokines in patients with different clinical stages and the healthy in the control group. According to genetic subtypes of HLA-DRB1 and HLA-DQB1, we also compared the concentration of cytokine (CK) in different genetic subtypes.
  
  
  RESULTS
  Eight HLA-DRB1 alleles and four HLA-DQB1 alleles were found. There were not significant differences between each allele in the concentration of IFN-γ, IL-2, IL-6, and IL-10.
  
  
  CONCLUSIONS
  HLA-DRB1*07, HLA-DQB1*02 and HLA-DQB1*03 were the differentially expressed gene in HPV16 infected patients with advanced cervical cancer. There may be correlations between the occurrence, development of cervical cancer and IFN-γ, IL-2, IL-6, IL-10.
authors.affiliation: Department of Gynecology, Binzhou People's Hospital Binzhou 256610, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pathology, Binzhou People's Hospital Binzhou 256610, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Thyroid and Breast Surgery, Affiliated Hospital of Binzhou Medical College Binzhou 256603, China.
authors.forename: Zhong-Ming
authors.initials: ZM
authors.lastname: Jia
authors.affiliation: Department of Gynecology, Binzhou People's Hospital Binzhou 256610, China.
authors.forename: Ji-Chang
authors.initials: JC
authors.lastname: Li
authors.affiliation: Department of Gynecology, Binzhou People's Hospital Binzhou 256610, China.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Dong
authors.affiliation: Department of Gynecology, Binzhou People's Hospital Binzhou 256610, China.
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Li
keywords: HLA-DQB1; HLA-DRB1; cervical cancer; cytokines; genetic polymorphism; hpv16