title: Proteomic analysis through larval development of Solea senegalensis flatfish.
abstract: The post-embryonic development of the Senegalese sole, Solea senegalensis, a flatfish of growing interest in fisheries and aquaculture, is associated with drastic morpho-physiological changes during metamorphosis. Although in the last two decades knowledge on sole culture has notably increased, especially in Southern Europe, its progress was restricted due to lack of methods to control reproduction, improve larval quality and increase juvenile disease resistance. A limited knowledge of the physiological, molecular and genetic mechanisms involved is at the base of such limitation. A proteomic study was carried out to explore the molecular events that occur during S. senegalensis ontogenesis. Protein expression changes were monitored in larvae from 5 to 21 dph by combining 2DE and protein identification with de novo MS/MS sequencing. An average of 6177 ± 282 spots was resolved in 2DE gels. Hierarchical cluster analysis of the 705 selected spots grouped them in eight patterns. Thirty-four proteins were identified and assigned biological functions including structure, metabolism highlighting energy metabolism, transport, protein folding, stress response, chromatin organization and regulation of gene expression. These changes provide a sequential description of the molecular events associated with the biochemical and biological transformations that occur during sole larval development.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Córdoba (UCO), Córdoba, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Chicano-Gálvez
authors.affiliation: IFAPA Centro El Toruño, J. Andalucía, Cádiz, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Asensio
authors.affiliation: IFAPA Centro El Toruño, J. Andalucía, Cádiz, Spain.
authors.forename: José Pedro
authors.initials: JP
authors.lastname: Cañavate
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Córdoba (UCO), Córdoba, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Alhama
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Córdoba (UCO), Córdoba, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: López-Barea
mesh_terms: D000818:Animals; D005412:Flatfishes / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
keywords: Animal proteomics; Energy metabolism; Flatfish; Larval development; Metamorphosis; Structural proteins