title: Development of mercury (II) ion biosensors based on mercury-specific oligonucleotide probes.
abstract: Mercury (II) ion (Hg(2+)) contamination can be accumulated along the food chain and cause serious threat to the public health. Plenty of research effort thus has been devoted to the development of fast, sensitive and selective biosensors for monitoring Hg(2+). Thymine was demonstrated to specifically combine with Hg(2+) and form a thymine-Hg(2+)-thymine (T-Hg(2+)-T) structure, with binding constant even higher than T-A Watson-Crick pair in DNA duplex. Recently, various novel Hg(2+) biosensors have been developed based on T-rich Mercury-Specific Oligonucleotide (MSO) probes, and exhibited advanced selectivity and excellent sensitivity for Hg(2+) detection. In this review, we explained recent development of MSO-based Hg(2+) biosensors mainly in 3 groups: fluorescent biosensors, colorimetric biosensors and electrochemical biosensors.
authors.affiliation: Laboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, PR China.
authors.forename: Lanying
authors.initials: L
authors.lastname: Li
authors.affiliation: Laboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, PR China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Laboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Laboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, PR China.
authors.forename: Shiping
authors.initials: S
authors.lastname: Song
authors.affiliation: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, PR China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zuo
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, 999 Hucheng Huan Road, Pudong District, Shanghai 201306, PR China. Electronic address: liug@simt.com.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Yan
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, 999 Hucheng Huan Road, Pudong District, Shanghai 201306, PR China.
authors.forename: Weijia
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Laboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques; D003124:Colorimetry; D004247:DNA / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D008628:Mercury / Q000737:chemistry / Q000302:isolation & purification*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D013941:Thymine / Q000737:chemistry
chemical_list: D015345:Oligonucleotide Probes; D006046:Gold; D004247:DNA; D008628:Mercury; D013941:Thymine
keywords: Biosensors, Hg(2+); Mercury contamination; Mercury-specific oligonucleotide; T–Hg(2+)–T