title: MicroRNAs in Salivary Exosome as Potential Biomarkers of Aging.
abstract: The aim of this study was to examine whether salivary exosomal miRNAs could be identified as aging biomarkers. Fifteen young healthy volunteers (median age, 21.0 years) and 13 old individuals (median age, 66.0 years) were recruited. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. In a microarray, 242 miRNAs were commonly detected in these two mixed samples. Based on the cut-off values of 2- or 0.5-fold changes (FC) and regulatory power for aging process, six candidate miRNAs (miR-24-3p, miR-371a-5p, miR-3175, miR-3162-5p, miR-671-5p, and miR-4667-5p) were selected. After comparing each total RNA obtained by the 15 young and 13 old individuals to validate the FC values using quantitative real-time PCR, miR-24-3p was identified as a novel candidate aging biomarker. This pilot study suggested that salivary exosomal miRNAs could be identified as candidate aging biomarkers. To confirm whether miR-24-3p in salivary exosomes are suitable biomarkers of aging, further validation research is required.
authors.affiliation: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. de17046@s.okadai.jp.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Machida
authors.affiliation: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. tomofu@md.okayama-u.ac.jp.|Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. tomofu@md.okayama-u.ac.jp.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Tomofuji
authors.affiliation: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. dekuni7@md.okayama-u.ac.jp.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ekuni
authors.affiliation: Center for Innovative Clinical Medicine, Okayama University Hospital, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. t-maru@md.okayama-u.ac.jp.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Maruyama
authors.affiliation: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. de17057@s.okadai.jp.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Yoneda
authors.affiliation: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. de18019@s.okayama-u.ac.jp.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kawabata
authors.affiliation: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. pc2o46pj@okayama-u.ac.jp.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Mizuno
authors.affiliation: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. plzs3rog@okayama-u.ac.jp.
authors.forename: Hisataka
authors.initials: H
authors.lastname: Miyai
authors.affiliation: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. de19013@s.okayama-u.ac.jp.
authors.forename: Muneyoshi
authors.initials: M
authors.lastname: Kunitomo
authors.affiliation: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. mmorita@md.okayama-u.ac.jp.
authors.forename: Manabu
authors.initials: M
authors.lastname: Morita
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000375:Aging / Q000235:genetics* / Q000378:metabolism*; D015415:Biomarkers; D019295:Computational Biology / Q000379:methods; D055354:Exosomes; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs; D008875:Middle Aged; D012372:ROC Curve; D012463:Saliva / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: aging; exosome; microRNA; saliva