title: Identification of CEACAM5 as a Biomarker for Prewarning and Prognosis in Gastric Cancer.
abstract: MGd1, a monoclonal antibody raised against gastric cancer cells, possesses a high degree of specificity for gastric cancer (GC). Here we identified that the antigen of MGd1 is CEACAM5, and used MGd1 to investigate the expression of CEACAM5 in non-GC and GC tissues (N=643), as a biomarker for prewarning and prognosis. The expression of CEACAM5 was detected by immunohistochemistry in numerous tissues; its clinicopathological correlation was statistically analyzed. CEACAM5 expression was increased progressively from normal gastric mucosa to chronic atrophic gastritis, intestinal metaplasia, dysplasia and finally to GC (p<0.05). In gastric precancerous lesions (intestinal metaplasia and dysplasia), CEACAM5-positive patients had a higher risk of developing GC as compared with CEACAM5-negative patients (OR = 12.68, p<0.001). Besides, CEACAM5 was found positively correlated with invasion depth of gastric adenocarcinoma (p<0.001). In survival analysis, CEACAM5 was demonstrated to be an independent prognostic predictor for patients with GC of clinical stage IIIA/IV (p=0.033). Our results demonstrate that CEACAM5 is a promising biomarker for GC prewarning and prognostic evaluation.
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China (JZ, NC, FZ, JD, YN, DF)
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Institute of Plastic Surgery of the Chinese PLA, Fourth Military Medical University, Xi'an, China (XF)
authors.forename: Xing
authors.initials: X
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China (JZ, NC, FZ, JD, YN, DF)
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China (JZ, NC, FZ, JD, YN, DF)
authors.forename: Fenli
authors.initials: F
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China (JZ, NC, FZ, JD, YN, DF)
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China (JZ, NC, FZ, JD, YN, DF)
authors.forename: Yongzhan
authors.initials: Y
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China (JZ, NC, FZ, JD, YN, DF)
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics / Q000401:mortality / Q000473:pathology; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002272:Carcinoembryonic Antigen / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D005757:Gastritis, Atrophic / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008679:Metaplasia / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D008875:Middle Aged; D009367:Neoplasm Staging; D011230:Precancerous Conditions / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D012306:Risk; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000401:mortality / Q000473:pathology; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; C527875:CEACAM5 protein, human; D002272:Carcinoembryonic Antigen; D058851:GPI-Linked Proteins
keywords: CEACAM5; biomarker; gastric cancer; immunohistochemistry; prognosis