title: Dynamic and social behaviors of human pluripotent stem cells.
abstract: Human pluripotent stem cells (hPSCs) can self-renew or differentiate to diverse cell types, thus providing a platform for basic and clinical applications. However, pluripotent stem cell populations are heterogeneous and functional properties at the single cell level are poorly documented leading to inefficiencies in differentiation and concerns regarding reproducibility and safety. Here, we use non-invasive time-lapse imaging to continuously examine hPSC maintenance and differentiation and to predict cell viability and fate. We document dynamic behaviors and social interactions that prospectively distinguish hPSC survival, self-renewal, and differentiation. Results highlight the molecular role of E-cadherin not only for cell-cell contact but also for clonal propagation of hPSCs. Results indicate that use of continuous time-lapse imaging can distinguish cellular heterogeneity with respect to pluripotency as well as a subset of karyotypic abnormalities whose dynamic properties were monitored.
authors.affiliation: Institute for Stem Cell Biology and Regenerative Medicine, School of Medicine, Stanford University, California, USA.|Department of Genetics, School of Medicine, Stanford University, California, USA.|Department of Obstetrics and Gynecology, School of Medicine, Stanford University, California, USA.
authors.forename: Smruti M
authors.initials: SM
authors.lastname: Phadnis
authors.affiliation: Department of Electrical Engineering, Stanford University, Stanford, California, USA.|Edward L. Ginzton Laboratory, Stanford University, Stanford, California, USA.
authors.forename: Nathan O
authors.initials: NO
authors.lastname: Loewke
authors.affiliation: Institute for Stem Cell Biology and Regenerative Medicine, School of Medicine, Stanford University, California, USA.
authors.forename: Ivan K
authors.initials: IK
authors.lastname: Dimov
authors.affiliation: Institute for Stem Cell Biology and Regenerative Medicine, School of Medicine, Stanford University, California, USA.
authors.forename: Sunil
authors.initials: S
authors.lastname: Pai
authors.affiliation: Department of Electrical Engineering, Stanford University, Stanford, California, USA.|Edward L. Ginzton Laboratory, Stanford University, Stanford, California, USA.
authors.forename: Christine E
authors.initials: CE
authors.lastname: Amwake
authors.affiliation: Department of Electrical Engineering, Stanford University, Stanford, California, USA.|Edward L. Ginzton Laboratory, Stanford University, Stanford, California, USA.
authors.forename: Olav
authors.initials: O
authors.lastname: Solgaard
authors.affiliation: Stanford Photonics Research Center, Department of Applied Physics, Stanford University, Stanford, California, USA.
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Baer
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Stanford University, California, USA.
authors.forename: Bertha
authors.initials: B
authors.lastname: Chen
authors.affiliation: Institute for Stem Cell Biology and Regenerative Medicine, School of Medicine, Stanford University, California, USA.|Department of Genetics, School of Medicine, Stanford University, California, USA.|Department of Obstetrics and Gynecology, School of Medicine, Stanford University, California, USA.
authors.forename: Renee A
authors.initials: RA
authors.lastname: Reijo Pera
mesh_terms: D015415:Biomarkers; D015820:Cadherins / Q000378:metabolism; D002450:Cell Communication; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D000066673:Cell Self Renewal; D002470:Cell Survival; D005260:Female; D006801:Humans; D059785:Karyotype; D008297:Male; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology*; D059008:Time-Lapse Imaging
chemical_list: D015415:Biomarkers; D015820:Cadherins