title: Mechanisms regulating spill-over of synaptic glutamate to extrasynaptic NMDA receptors in mouse substantia nigra dopaminergic neurons.
abstract: N-Methyl-D-aspartate glutamate receptors (NMDARs) contribute to neural development, plasticity and survival, but they are also linked with neurodegeneration. NMDARs at synapses are activated by coincident glutamate release and depolarization. NMDARs distal to synapses can sometimes be recruited by 'spill-over' of glutamate during high-frequency synaptic stimulation or when glutamate uptake is compromised, and this influences the shape of NMDAR-mediated postsynaptic responses. In substantia nigra dopamine neurons, activation of NMDARs beyond the synapse during different frequencies of presynaptic stimulation has not been explored, even though excitatory afferents from the subthalamic nucleus show a range of firing frequencies, and these frequencies change in human and experimental Parkinson's disease. This study reports that high-frequency stimulation (80 Hz/200 ms) evoked NMDAR-excitatory postsynaptic currents (EPSCs) that were larger and longer lasting than those evoked by single stimuli at low frequency (0.1 Hz). MK-801, which irreversibly blocked NMDAR-EPSCs activated during 0.1-Hz stimulation, left a proportion of NMDAR-EPSCs that could be activated by 80-Hz stimulation and that may represent activity of NMDARs distal to synapses. TBOA, which blocks glutamate transporters, significantly increased NMDAR-EPSCs in response to 80-Hz stimulation, particularly when metabotropic glutamate receptors (mGluRs) were also blocked, indicating that recruitment of NMDARs distal to synapses is regulated by glutamate transporters and mGluRs. These regulatory mechanisms may be essential in the substantia nigra for restricting glutamate diffusion from synaptic sites and keeping NMDAR-EPSCs in dopamine neurons relatively small and fast. Failure of glutamate transporters may contribute to the declining health of dopamine neurons during pathological conditions.
authors.affiliation: Department of Physiology, Development & Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK.
authors.forename: A R
authors.initials: AR
authors.lastname: Wild
authors.affiliation: Department of Physiology, Development & Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK.
authors.forename: M
authors.initials: M
authors.lastname: Bollands
authors.affiliation: Department of Physiology, Development & Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK.
authors.forename: P G
authors.initials: PG
authors.lastname: Morris
authors.affiliation: Department of Physiology, Development & Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK.
authors.forename: S
authors.initials: S
authors.lastname: Jones
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001224:Aspartic Acid / Q000494:pharmacology; D016291:Dizocilpine Maleate / Q000494:pharmacology; D059290:Dopaminergic Neurons / Q000187:drug effects / Q000502:physiology*; D004558:Electric Stimulation; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D018698:Glutamic Acid / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000502:physiology*; D013378:Substantia Nigra / Q000502:physiology*; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D014966:Xanthenes / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D018691:Excitatory Amino Acid Antagonists; C114624:LY 341495; D016194:Receptors, N-Methyl-D-Aspartate; D014966:Xanthenes; C120673:benzyloxyaspartate; D001224:Aspartic Acid; D018698:Glutamic Acid; D016291:Dizocilpine Maleate
keywords: NMDA glutamate receptor; glutamate transporter; metabotropic glutamate receptor; substantia nigra pars compacta