title: Genome wide microarray based expression profiles associated with BmNPV resistance and susceptibility in Indian silkworm races of Bombyx mori.
abstract: The molecular mechanism involved in BmNPV resistance was investigated using a genome wide microarray in midgut tissue of Indian silkworm Bombyx mori. In resistant race (Sarupat), 735 genes up-regulated and 589 genes down-regulated at 12 h post BmNPV infection. Similarly, in case of susceptible race (CSR-2), 2183 genes up-regulated and 2115 genes down-regulated. Among these, nine up-regulated and eight down-regulated genes were validated using real-time qPCR analysis. In Sarupat, vacuolar protein sorting associated, Xfin-like protein and carboxypeptidase E-like protein genes significantly up-regulated in infected midgut; prominently down-regulated genes were glutamate receptor ionotropic kainite 2-like, BTB/POZ domain and transferrin. Considerably up-regulated genes in the CSR-2 were peptidoglycan recognition protein S6 precursor and rapamycin while the conspicuous down-regulated genes were facilitated trehalose transporter and zinc transporter ZIP1-like gene. The up-regulation of genes in resistant race after BmNPV infection indicates their possible role in antiviral immune response.
authors.affiliation: Genomics Division, Seribiotech Research Laboratory, Carmelaram Post, Kodathi, Bangalore 560 035, India.
authors.forename: Govindaraj
authors.initials: G
authors.lastname: Lekha
authors.affiliation: Genomics Division, Seribiotech Research Laboratory, Carmelaram Post, Kodathi, Bangalore 560 035, India.
authors.forename: Tania
authors.initials: T
authors.lastname: Gupta
authors.affiliation: Genomics Division, Seribiotech Research Laboratory, Carmelaram Post, Kodathi, Bangalore 560 035, India.
authors.forename: Arvind K
authors.initials: AK
authors.lastname: Awasthi
authors.affiliation: Genomics Division, Seribiotech Research Laboratory, Carmelaram Post, Kodathi, Bangalore 560 035, India.
authors.forename: Geetha N
authors.initials: GN
authors.lastname: Murthy
authors.affiliation: Genomics Division, Seribiotech Research Laboratory, Carmelaram Post, Kodathi, Bangalore 560 035, India.
authors.forename: Kanika
authors.initials: K
authors.lastname: Trivedy
authors.affiliation: Genomics Division, Seribiotech Research Laboratory, Carmelaram Post, Kodathi, Bangalore 560 035, India. Electronic address: kmpvel@yahoo.com.
authors.forename: Kangayam M
authors.initials: KM
authors.lastname: Ponnuvel
mesh_terms: D000818:Animals; D012831:Bombyx / Q000145:classification / Q000235:genetics* / Q000821:virology; D060467:Disease Resistance / Q000235:genetics*; D015536:Down-Regulation; D020869:Gene Expression Profiling / Q000379:methods*; D063990:Gene Ontology; D017344:Genes, Insect / Q000235:genetics; D049750:Genome, Insect / Q000235:genetics*; D019476:Insect Proteins / Q000145:classification / Q000235:genetics; D017924:Nucleopolyhedroviruses / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D015854:Up-Regulation
chemical_list: D019476:Insect Proteins
keywords: Antiviral; BmNPV; CSR-2; Down-regulated; Genome wide microarray; Immune response; Molecular mechanism; Resistant; Sarupat; Susceptible; Up-regulated