title: Biofilm, pathogenesis and prevention--a journey to break the wall: a review.
abstract: Biofilms contain group(s) of microorganisms that are found to be associated with the biotic and abiotic surfaces. Biofilms contain either homogenous or heterogeneous populations of bacteria which remain in the matrix made up of extracellular polymeric substances secreted by constituent population of the biofilm. Biofilms can be either single or multilayered. Biofilms are an increasing issue of concern that is gaining importance with each passing day. Due to the ubiquitous nature of biofilms, it is difficult to eradicate them. It has been seen that many infectious diseases harbour biofilms of bacterial pathogens as the reservoir of persisting infections which can prove fatal at times. The presence of biofilms can be seen in diseases like endocarditis, cystic fibrosis, periodontitis, rhinosinusitis and osteomyelitis. The presence of biofilms has been mostly seen in medical implants and urinary catheters. Various signalling events including two-component signalling, extra cytoplasmic function and quorum sensing are involved in the formation of biofilms. The presence of an extracellular polymeric matrix in biofilms makes it difficult for the antimicrobials to act on them and make the bacteria tolerant to antibiotics and other drugs. The aim of this review was to discuss about the basic formation of a biofilm, various signalling cascades involved in biofilm formation, possible mechanisms of drug resistance in biofilms and recent therapeutic approaches involved in successful eradication of biofilms.
authors.affiliation: Department of Molecular Biology and Bioinformatics, Tripura University (A Central University), Suryamaninagar, Agartala, Tripura, 799022, India. preea19@gmail.com.
authors.forename: Priya
authors.initials: P
authors.lastname: Gupta
authors.affiliation: Department of Molecular Biology and Bioinformatics, Tripura University (A Central University), Suryamaninagar, Agartala, Tripura, 799022, India. cusubhasis@gmail.com.
authors.forename: Subhasis
authors.initials: S
authors.lastname: Sarkar
authors.affiliation: Department of Biotechnology, Mount Carmel College, Bangalore, 560 052, India. bannhi.das@gmail.com.
authors.forename: Bannhi
authors.initials: B
authors.lastname: Das
authors.affiliation: Department of Molecular Biology and Bioinformatics, Tripura University (A Central University), Suryamaninagar, Agartala, Tripura, 799022, India. sbhattacharjee@gmail.com.
authors.forename: Surajit
authors.initials: S
authors.lastname: Bhattacharjee
authors.affiliation: Department of Microbiology, Assam Don Bosco University, Guwahati, Assam, 781017, India. tribedi.prosun@gmail.com.
authors.forename: Prosun
authors.initials: P
authors.lastname: Tribedi
mesh_terms: D001419:Bacteria / Q000472:pathogenicity; D001424:Bacterial Infections / Q000517:prevention & control*; D018407:Bacterial Physiological Phenomena; D018441:Biofilms; D024881:Drug Resistance, Bacterial / Q000502:physiology; D006801:Humans; D053038:Quorum Sensing; D015398:Signal Transduction
keywords: Antibiofilm therapeutics; Biofilm formation; Drug tolerance; Pathogenesis