title: CD4 T-cell function assay using Cylex ImmuKnow and lymphocyte subset recovery following allogeneic hematopoietic stem cell transplantation.
abstract: We analyzed CD4 T-lymphocytes ATP levels along with lymphocyte subsets in 160 samples from 111 post-allogeneic hematopoietic stem cell transplantation (alloHSCT) patients. In patients with stable status, ImmuKnow levels changed over time and the 6-month post-alloHSCT levels were significantly higher than those tested within 6 months post-alloHSCT (P < 0.001). Although, ImmuKnow levels for acute graft-versus-host disease (GVHD) or infection episodes were not significantly different compared to those for stable alloHSCT, the levels were correlated with specific lymphocyte subpopulations at different times; the results within 6 months post-alloHSCT showed low positive correlation with natural killer cell count (r = 0.328) (P < 0.05) and the values tested later than 6 months post-alloHSCT were positively correlated with CD4 T cell count (r = 0.425) (P < 0.05). Two patients who developed acute GVHD and two who experienced an infection episode showed increased ImmuKnow levels in sequential tests. The combined test of ImmuKnow levels and lymphocyte subsets may be helpful for immune monitoring following alloHSCT.
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Jo
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Jihyang
authors.initials: J
authors.lastname: Lim
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Yonggoo
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Kyungja
authors.initials: K
authors.lastname: Han
authors.affiliation: Department of Hematology, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Woo-Sung
authors.initials: WS
authors.lastname: Min
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: ejoh@catholic.ac.kr.
authors.forename: Eun-Jee
authors.initials: EJ
authors.lastname: Oh
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis* / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007118:Immunoassay; D007239:Infections / Q000175:diagnosis* / Q000276:immunology; D007519:Isoantigens / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D007519:Isoantigens
keywords: Acute graft versus host disease; Allogeneic hematopoietic stem cell transplantation; CD4; Immune function