title: [Establishment and identification of Jurkat cell xenograft mouse models with over-expression of C-terminal Src kinase binding protein].
abstract: OBJECTIVE
  To establish xenograft mouse models of Jurkat T-leukemia cells over-expressing C-terminal Src kinase binding protein (CBP).
  
  
  METHODS
  The 5-week-old female BALB/c-nu mice were randomly divided into blank control group, normal Jurkat cell control group, empty virus-transfected Jurkat cell control group and CBP over-expression model group, 5 mice in each group. The mice were subcutaneously injected 1×10(7)/0.1 mL Jurkat cells in axillary area. The tumor tissues of mouse models were weighed and then subjected to HE staining to observe the pathological changes of tumor tissues. The proliferation of Jurkat cells in the peripheral blood of mice was detected by flow cytometry, and the interleukin 2 (IL-2) levels in mouse sera were determined with ELISA.
  
  
  RESULTS
  The volume of tumor tissues in the CBP over-expression model group was smaller than that in the control groups, so was the mass of tumor tissues. HE staining showed the proliferation of Jurkat cells in tumor tissues of the model group. The proliferation rate of Jurkat cells in the peripheral blood and IL-2 levels in the sera of the CBP over-expression model group were lower than those in the normal Jurkat cell control group and the empty virus-transfected Jurkat cell control group.
  
  
  CONCLUSIONS
  The mouse models of Jurkat T-leukemia cells over-expressing CBP have been established successfully. Up-regulated CBP has inhibitory effects on the proliferation of Jurkat cells and IL-2 secretion.
authors.affiliation: Department of Immunology, Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Immunology, Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Immunology, Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Immunology, Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Minrui
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Blood Transfusion, Hospital Affiliated to Medical College, Qingdao University, Qingdao 266071, China.
authors.forename: Shuchao
authors.initials: S
authors.lastname: Zhang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000818:Animals; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000097:blood; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000378:metabolism / Q000473:pathology*; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D007376:Interleukin-2; D008565:Membrane Proteins; C498710:PAG1 protein, human