title: Phytochemical Screening: Antioxidant and Antibacterial Properties of Potamogeton Species in Order to Obtain Valuable Feed Additives.
abstract: The alcoholic extracts from three submerged perennial plants Potamogeton crispus L., P. pusillus L. and P. pectinatus L. were analyzed by gas chromatography-mass spectrometry coupled with solid phase microextraction (SPME-GC/MS) and by High Performance Liquid Chromatography (HPLC) and their volatile fingerprint and polyphenols composition was mutually compared. Twenty-nine chemical compounds were detected and identified in ethanolic and methanolic extracts; the highest abundance (over 5%) in descending order, was detected for 9,9-dimethyl-8,10- dioxapentacyclo (5,3,0(2,5) 0(3,5,)0 (3,6) decane (21.65%), phenol 2,6 bis (1,1 dimethyletyl) 4-1-methylpropil (20.8%), pentadecanoic acid (14.3%), 2-(5-chloro-2-Methoxyphenyl) pyrrole (8.66%), propanedioic (malonic) acid 2-(4-methylphenyl) sulfonyl ethylidene (5.77%), 2 hydroxy-3 tert butyl-5-isopropyl-6 methyl phenyl ketone (5.76%). The highest total polyphenols and flavonoids content was found in the methanolic extract of P. crispus (112.5±0.5 mg tannic acid/g dry extract; 64.2±1.2 mg quercitin/g dry extract). Antioxidant activities (2,2-difenil-1-picrilhidrazil, hydrogen peroxide and reducing power assays) of obtained extracts are comparable with the standard compounds, butylated hydroxytoluene, rutin and ascorbic acid. Antibacterial efficiency of methanolic extracts was notably demonstrated against Gram negative (Escherichia coli, Enterobacter hormaechei) and Gram positive bacteria (Enterococcus casseliflavus). The data reported for the first time for Romanian Potamogeton species, provides extensive support for the chemical investigations of these plants of the aquatic anthropogene ecosystems in order to obtain valuable bioadditives for animal feed and/or pharmaceutical/food industry.
authors.affiliation: Dunărea de Jos University Galaţi, Faculty of Food Science and Engeneering.
authors.forename: Paul
authors.initials: P
authors.lastname: Lupoae
authors.forename: Victor
authors.initials: V
authors.lastname: Cristea
authors.forename: Daniela
authors.initials: D
authors.lastname: Borda
authors.forename: Mariana
authors.initials: M
authors.lastname: Lupoae
authors.forename: Gabriela
authors.initials: G
authors.lastname: Gurau
authors.forename: Rodica Mihaela
authors.initials: RM
authors.lastname: Dinica
mesh_terms: D000821:Animal Feed; D000900:Anti-Bacterial Agents; D000975:Antioxidants; D002851:Chromatography, High Pressure Liquid; D024881:Drug Resistance, Bacterial; D004754:Enterobacter / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification* / Q000494:pharmacology; D005503:Food Additives; D008401:Gas Chromatography-Mass Spectrometry; D000432:Methanol; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D059808:Polyphenols / Q000032:analysis* / Q000302:isolation & purification* / Q000494:pharmacology; D031821:Potamogetonaceae / Q000737:chemistry*; D052617:Solid Phase Microextraction / Q000379:methods; D014835:Volatilization
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005419:Flavonoids; D005503:Food Additives; D010936:Plant Extracts; D059808:Polyphenols; D000432:Methanol