title: Resveratrol and curcumin ameliorate di-(2-ethylhexyl) phthalate induced testicular injury in rats.
abstract: The present study aimed to evaluate the protective role of resveratrol and curcumin on oxidative testicular damage induced by di-(2-ethylhexyl) phthalate (DEHP). Male Wistar rats were divided into six groups; three groups received oral daily doses of DEHP (2g/kgBW) for 45days to induce testicular injury. Two of these groups received either resveratrol (80mg/kgBW) or curcumin (200mg/kgBW) orally for 30days before and 45days after DEHP administration. A vehicle-treated control group was also included. Another two groups of rats received either resveratrol or curcumin alone. Oxidative damage was observed by decreased levels of total antioxidant capacity (TAC) and glutathione (GSH) and increased malondialdehyde (MDA) level in the testes of DEHP-administered rats. Serum testosterone level as well as testicular marker enzymes activities; acid and alkaline phosphatases (ACP and ALP) and lactate dehydrogenase (LDH) showed severe declines. DEHP administration caused significant increases in the testicular gene expression levels of Nrf2, HO-1, HSP60, HSP70 and HSP90 as well as a significant decrease in c-Kit protein when compared with the control group. Histopathological observations provided evidence for the biochemical and molecular analysis. These DEHP-induced pathological alterations were attenuated by pretreatment with resveratrol and curcumin. We conclude that DEHP-induced injuries in biochemical, molecular and histological structure of testis were recovered by pretreatment with resveratrol and curcumin. The chemoprotective effects of these compounds may be due to their intrinsic antioxidant properties along with boosting Nrf2, HSP 60, HSP 70 and HSP 90 gene expression levels and as such may be useful potential tools in combating DEHP-induced testicular dysfunction.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: Amal Ahmed
authors.initials: AA
authors.lastname: Abd El-Fattah
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, October 6 University, Cairo, Egypt.
authors.forename: Atef Tadros
authors.initials: AT
authors.lastname: Fahim
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo, Egypt. Electronic address: nerminsadik@yahoo.com.
authors.forename: Nermin Abdel Hamid
authors.initials: NAH
authors.lastname: Sadik
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, October 6 University, Cairo, Egypt.
authors.forename: Bassam Mohamed
authors.initials: BM
authors.lastname: Ali
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003474:Curcumin / Q000494:pharmacology / Q000627:therapeutic use*; D004051:Diethylhexyl Phthalate; D015870:Gene Expression / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*; D013733:Testicular Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000097:blood
chemical_list: D000975:Antioxidants; D006360:Heat-Shock Proteins; D020011:Protective Agents; D013267:Stilbenes; D013739:Testosterone; D008315:Malondialdehyde; D004051:Diethylhexyl Phthalate; D051547:Heme Oxygenase-1; D000469:Alkaline Phosphatase; D005978:Glutathione; D003474:Curcumin; D000077185:Resveratrol
keywords: Curcumin; DEHP; HO-1; HSPs; Nrf2; Rat; Resveratrol; Testes