title: [In vitro differentiation of human umbilical cord blood mononuclear cells into mature mast cells induced by cytokines].
abstract: OBJECTIVE
  To isolate and induce human umbilical cord blood mononuclear cells to differentiate into mature mast cells of high purity.
  
  
  METHODS
  Human umbilical cord blood mononuclear cells were differentiated into mature mast cells by the treatment of recombinant human stem cell factor (rhSCF) and recombinant human interleukin 6 (rhIL-6). The cultured cells at different time points were stained with toluidine blue for the detection of anti-FcepsilonRI and the maturity of mast cells was detected by flow cytometry. After the mature mast cells were stimulated with allergen, the levels of histamine and tryptase in the supernatant were determined.
  
  
  RESULTS
  The cells started to express FcepsilonRI receptor after 2-week treatment of rhSCF and rhIL-6. After 3 weeks, the amount of FcepsilonRI receptor reached its peak accompanied by increased intracellular basophilic granules. The mature mast cells released tryptase and histamine effectively after allergen challenge.
  
  
  CONCLUSIONS
  Human umbilical cord blood mononuclear cells can be differentiated into mature mast cells by the treatment of rhSCF and rhIL-6. The mature mast cells may be used for the study of allergenicity in vitro.
authors.affiliation: Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology/State Key Laboratory of Respiratory Diseases, Second Affiliated Hospital, Guangzhou Medical University, Guangzhou 510260, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology/State Key Laboratory of Respiratory Diseases, Second Affiliated Hospital, Guangzhou Medical University, Guangzhou 510260, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Obstetrics, Second Affiliated Hospital, Guangzhou Medical University, Guangzhou 510260, China.
authors.forename: Lanzhen
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology/State Key Laboratory of Respiratory Diseases, Second Affiliated Hospital, Guangzhou Medical University, Guangzhou 510260, China.
authors.forename: Zhaoyu
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology/State Key Laboratory of Respiratory Diseases, Second Affiliated Hospital, Guangzhou Medical University, Guangzhou 510260, China.
authors.forename: Zehong
authors.initials: Z
authors.lastname: Zou
authors.affiliation: Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology/State Key Laboratory of Respiratory Diseases, Second Affiliated Hospital, Guangzhou Medical University, Guangzhou 510260, China.
authors.forename: Ailin
authors.initials: A
authors.lastname: Tao
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D006636:Histamine Release; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008407:Mast Cells / Q000166:cytology*; D011994:Recombinant Proteins / Q000494:pharmacology; D019089:Stem Cell Factor / Q000494:pharmacology*; D053802:Tryptases / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D015850:Interleukin-6; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D053802:Tryptases