title: Venous versus arterial iron administration in haemodialysis. Influence on erythrocytes antioxidant parameters.
abstract: Introduction Intravenous iron administration in patients treated by haemodialysis for end stage renal disease can exacerbate oxidative stress by increasing the level of free redox active iron. A way to reduce the impact of iron on oxidative stress in haemodialysis patients may be the administration of iron through arterial extracorporeal circuit. Objective The aim of our study was to compare the influence of iron route of administration (venous versus arterial extracorporeal circuit infusion) on antioxidant parameters in red blood cells of haemodialysis patients in order to clarify if arterial iron administration can have positive impacts related to iron induced oxidative stress. Method Twenty stable patients on regular haemodialysis treatment were selected for the study. They were investigated in a cross-over design at 3 mid-week HD sessions, one week apart, without iron [HD basal] and with either IV infusion of 100mg iron sucrose over the first 20 minutes of HD session, via venous line [HDvenous], or the same solution infused on the arterial extracorporeal circulation [HDarterial]. Blood samples were drawn at 0 min, 40 min and 270 min. Erythrocytes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) activity, non-protein thiol levels and total antioxidant capacity (TEAC) were analysed. Conclusion Haemodialysis significantly decreases the total antioxidant activity in erythrocytes. Iron supplementation, through venous or arterial extracorporeal route has no impact on the total antioxidant activity in red blood cells. Venous iron administration increases GPx activity in erythrocytes suggesting increased lipid peroxidation compared with arterial extracorporeal administration.
authors.affiliation: "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
authors.forename: C B
authors.initials: CB
authors.lastname: Dogaru
authors.affiliation: "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
authors.forename: C
authors.initials: C
authors.lastname: Capusa
authors.affiliation: "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania ; R&D Irist Labmed, Bucharest, Romania.
authors.forename: L
authors.initials: L
authors.lastname: Gaman
authors.affiliation: "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
authors.forename: E
authors.initials: E
authors.lastname: Torac
authors.affiliation: "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
authors.forename: D
authors.initials: D
authors.lastname: Lixandru
authors.affiliation: "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania ; R&D Irist Labmed, Bucharest, Romania.
authors.forename: M
authors.initials: M
authors.lastname: Gilca
authors.affiliation: "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania ; R&D Irist Labmed, Bucharest, Romania.
authors.forename: L
authors.initials: L
authors.lastname: Iosif
authors.affiliation: "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
authors.forename: C
authors.initials: C
authors.lastname: Muscurel
authors.affiliation: "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania ; R&D Irist Labmed, Bucharest, Romania.
authors.forename: I
authors.initials: I
authors.lastname: Stoian
authors.affiliation: "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
authors.forename: G
authors.initials: G
authors.lastname: Mircescu
authors.affiliation: "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
authors.forename: V
authors.initials: V
authors.lastname: Atanasiu
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007269:Injections, Intra-Arterial; D007275:Injections, Intravenous; D007501:Iron / Q000008:administration & dosage* / Q000494:pharmacology*; D006435:Renal Dialysis; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D013438:Sulfhydryl Compounds; D007501:Iron; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: antioxidant parameters; erythrocytes; haemodialysis