title: Electrochemical enzyme-less urea sensor based on nano-tin oxide synthesized by hydrothermal technique.
abstract: Nano-Tin oxide was synthesized using hydrothermal method at 150 °C for 6 h and then thin films were deposited by electrophoretic method at an optimized voltage of 100 V for 5 min on electropolished aluminum substrate. Spherical particles of about 30-50 nm diameters are observed with partial agglomeration when observed under electron microscope, which are tetragonal rutile structure. XPS results showed peaks related to Sn 4d, Sn 3d, O 1s & C 1s with spin-orbit splitting of 8.4 eV for Sn 3d. Feasibility studies of enzyme less urea sensing characteristics of nano-tin oxide thin films are exhibited herein. The deposited films have been used for enzyme less urea sensing from 1 to 20 mM concentration in buffer solution. The sensors were characterized electrochemically to obtain cyclic voltammogram as a function of urea concentration and scan rate. The sensitivity is estimated as 18.9 μA/mM below 5 mM and 2.31 μA/mM above 5 mM with a limit of detection of 0.6 mM.
authors.affiliation: Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, 110025, India. Electronic address: saansari@jmi.ac.in.
authors.forename: S G
authors.initials: SG
authors.lastname: Ansari
authors.affiliation: Department of Applied Medical Science, RCC, King Saud University, 11437, Riyadh, Saudi Arabia; Department of Biomedical Engineering, Faculty of Engineering, Helwan University, P.O. Box, 11792, Helwan, Egypt. Electronic address: menhfefnew@hotmail.com.
authors.forename: H
authors.initials: H
authors.lastname: Fouad
authors.affiliation: School of Chemical Engineering, Jeonbuk National University, Jeonju, 561 756, Republic of Korea. Electronic address: hsshin@jbnu.ac.kr.
authors.forename: Hyung-Shik
authors.initials: HS
authors.lastname: Shin
authors.affiliation: Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, 110025, India.
authors.forename: Z A
authors.initials: ZA
authors.lastname: Ansari
mesh_terms: D060326:Chemistry Techniques, Synthetic; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D056951:Photoelectron Spectroscopy; D012680:Sensitivity and Specificity; D017971:Tin Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D014508:Urea / Q000032:analysis*
chemical_list: D004798:Enzymes; D017971:Tin Compounds; D014508:Urea; C045358:stannic oxide
keywords: Electrophoretic deposition; Enzyme-less; Hydrothermal; Surface composition; Tin oxide; Urea sensor