title: FTIR and SEM analysis applied in tissue engineering for root recovering surgery.
abstract: Gingival recession is defined by the displacement of the gingival margin in the apical direction, which overcomes the cementum enamel junction. The etiology of gingival retraction is related to tissue inflammation caused by the accumulation of biofilm, by trauma from brushing action. Aesthetic periodontal surgery aims to return the root coverage to aesthetic harmony, and reduce the risk of periodontal disease and caries. To assist in the root coverage process, the porcine collagen matrix (PCM) has been widely studied. The objectives of this study are to identify the types of collagen that make up the PCM and analyze their morphology. For this, five PCM fragments, 2 mm (thickness) × 2.6 mm (width), were analyzed with the aid of scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The analysis by SEM showed that the PCM consists of two layers; the surface layer is compact, low porosity, and smooth surface, and a foamed underlying layer has high porosity. Through FTIR we identified that the surface and underlying layers are composed of collagen types I and III, respectively. This biomaterial is conducive to root coverage; it allows adsorption and cell proliferation following the matrix resorption and periodontal tissue neoformation. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1326-1329, 2017.
authors.affiliation: Laser Therapy and Photobiology Center (CELAFO), UNIVAP, São José dos Campos, SP, Brazil.
authors.forename: Davidson Ribeiro
authors.initials: DR
authors.lastname: Costa
authors.affiliation: Laser Therapy and Photobiology Center (CELAFO), UNIVAP, São José dos Campos, SP, Brazil.
authors.forename: Renata Amadei
authors.initials: RA
authors.lastname: Nicolau
authors.affiliation: Laser Therapy and Photobiology Center (CELAFO), UNIVAP, São José dos Campos, SP, Brazil.
authors.forename: David Ribeiro
authors.initials: DR
authors.lastname: Costa
authors.affiliation: Nanosensors Laboratory, UNIVAP, São José dos Campos, SP, Brazil.
authors.forename: Leandro José
authors.initials: LJ
authors.lastname: Raniero
authors.affiliation: Department of Life Science and Biotechnology, UNIVAP, São José dos Campos, SP, Brazil.
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D005881:Gingiva / Q000378:metabolism / Q000601:surgery / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods; D010510:Periodontal Diseases / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods; D013552:Swine; D014092:Tooth Root / Q000378:metabolism / Q000601:surgery / Q000648:ultrastructure
keywords: Fourier transform infrared spectroscopy; gingival retraction; porcine collagen matrix; scanning electron microscopy; tissue engineering