title: Study of the inflammatory response of immunocytes to polymerized porcine hemoglobin (pPolyHb).
abstract: Polymerized porcine hemoglobin (pPolyHb), which was synthesized from chemically modified porcine hemoglobin, can carry and deliver oxygen to tissues in addition to restoring intravascular volume. Assessment of the inflammatory response generated in the host is a part of the overall safety evaluation of pPolyHb. In this study, we measured the levels of IL-1β, IL-10, and CD11b/CD18 in response to pPolyHb stimulation, both in vivo (in rats) and in vitro. Our results suggest that the levels of these indicators are not statistically changed by pPolyHb, indicating that pPolyHb is not immunotoxic to cells and animals in this respect.
authors.affiliation: a The First Affiliated Hospital of Xi'an Jiaotong University , Xi'an , P. R. China.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Zhu
authors.affiliation: b The Reproductive Centre, Tangdu Hospital, The Forth Military Medical University , Xi'an , P.R.China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: c College of Life Science, Northwest University , Xi'an , P. R. China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Xin
authors.affiliation: c College of Life Science, Northwest University , Xi'an , P. R. China.
authors.forename: Zhilan
authors.initials: Z
authors.lastname: Xie
authors.affiliation: c College of Life Science, Northwest University , Xi'an , P. R. China.
authors.forename: Boya
authors.initials: B
authors.lastname: Su
authors.affiliation: c College of Life Science, Northwest University , Xi'an , P. R. China.
authors.forename: Chengbin
authors.initials: C
authors.lastname: Yan
authors.affiliation: c College of Life Science, Northwest University , Xi'an , P. R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.affiliation: c College of Life Science, Northwest University , Xi'an , P. R. China.|d National Engineering Research Center for Miniaturized Detection Systems, Northwest University , Xi'an , P. R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: c College of Life Science, Northwest University , Xi'an , P. R. China.|d National Engineering Research Center for Miniaturized Detection Systems, Northwest University , Xi'an , P. R. China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D018821:CD18 Antigens / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006454:Hemoglobins / Q000009:adverse effects*; D016753:Interleukin-10 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D012449:Safety; D012771:Shock, Hemorrhagic / Q000276:immunology; D013552:Swine
chemical_list: D039481:CD11b Antigen; D018821:CD18 Antigens; D006454:Hemoglobins; D053583:Interleukin-1beta; C079129:hemoglobin polymer; D016753:Interleukin-10
keywords: CD11b/CD18; inflammatory response; interleukin-10; interleukin-1β; polymerized porcine hemoglobin