title: Alkaloids in Erythrina by UPLC-ESI-MS and In Vivo Hypotensive Potential of Extractive Preparations.
abstract: Erythrina species are used in popular medicine as sedative, anxiolytic, anti-inflammatory, and antihypertensive. In this work, we investigated the chemical composition of extracts obtained from leaves of E. falcata and E. crista-galli. The hypotensive potential of E. falcata and the mechanism of action were also studied. The extracts were obtained by maceration and infusion. The total content of phenolic compounds and flavonoids was estimated by spectrophotometric methods. The chemical constituents were studied performing a chromatographic analysis by UPLC-ESI-MS. For in vivo protocols, blood pressure and heart rate were measured by the invasive hemodynamic monitoring method. Different concentrations of extracts and drugs such as L-NAME, losartan, hexamethonium, and propranolol were administrated i.v. The results of total phenolic contents for E. falcata and E. crista-galli were 1.3193-1.4989 mgGAE/mL for maceration and 0.8771-0.9506 mgGAE/mL for infusion. In total flavonoids, the content was 7.7829-8.1976 mg RE/g for maceration and 9.3471-10.4765 RE mg/g for infusion. The chemical composition was based on alkaloids, suggesting the presence of erythristemine, 11β-methoxyglucoerysodine, erysothiopine, 11β-hydroxyerysodine-glucose, and 11-hydroxyerysotinone-rhamnoside. A potent dose-dependent hypotensive effect was observed for E. falcata, which may be related to the route of β-adrenergic receptors.
authors.affiliation: Programa de Pós-graduação em Bioquímica, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil ; Laboratório de Desenvolvimento e Controle de Qualidade de Medicamentos, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil.
authors.forename: Liara
authors.initials: L
authors.lastname: Merlugo
authors.affiliation: Laboratório de Desenvolvimento e Controle de Qualidade de Medicamentos, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil.
authors.forename: Marí C
authors.initials: MC
authors.lastname: Santos
authors.affiliation: Programa de Pós-graduação em Bioquímica, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil ; Laboratório de Fisiologia Cardiovascular, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil.
authors.forename: Liane S
authors.initials: LS
authors.lastname: Sant'Anna
authors.affiliation: Laboratório de Desenvolvimento e Controle de Qualidade de Medicamentos, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil.
authors.forename: Everson W F
authors.initials: EW
authors.lastname: Cordeiro
authors.affiliation: Laboratório de Desenvolvimento e Controle de Qualidade de Medicamentos, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil.
authors.forename: Luiz A C
authors.initials: LA
authors.lastname: Batista
authors.affiliation: Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Silvia T S
authors.initials: ST
authors.lastname: Miotto
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga 2752, 90610-000 Porto Alegre, RS, Brazil.
authors.forename: Cássia V
authors.initials: CV
authors.lastname: Garcia
authors.affiliation: Programa de Pós-graduação em Bioquímica, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil ; Laboratório de Fisiologia Cardiovascular, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil.
authors.forename: Cleci M
authors.initials: CM
authors.lastname: Moreira
authors.affiliation: Programa de Pós-graduação em Bioquímica, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil ; Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga 2752, 90610-000 Porto Alegre, RS, Brazil.
authors.forename: Andreas S L
authors.initials: AS
authors.lastname: Mendez