title: Multicenter evaluation of the Verigene Gram-negative blood culture nucleic acid test for rapid detection of bacteria and resistance determinants in positive blood cultures.
abstract: The Verigene Gram-Negative Blood Culture Nucleic Acid Test (BC-GN) is a microarray-based assay that enables rapid detection of 9 common Gram-negative bacteria and 6 resistance determinants directly from positive blood cultures. We compared the performance of BC-GN with currently used automated systems, testing 141 clinical blood cultures and 205 spiked blood cultures. For identification of BC-GN target organisms in clinical and spiked blood cultures, the BC-GN assay showed 98.5% (130/132) and 98.9% (182/184) concordance, respectively. Of 140 resistance genes positively detected in clinical and spiked blood cultures with the BC-GN test, 139 (99.3%) were confirmed by PCR, and the detection results were consistent with the resistance phenotypes observed. The BC-GN assay, thus, can potentially improve care for sepsis patients by enabling timely detection and targeted antimicrobial therapy.
authors.affiliation: Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. Electronic address: nuno@nagasaki-u.ac.jp.
authors.forename: Naoki
authors.initials: N
authors.lastname: Uno
authors.affiliation: Department of Clinical Laboratory Medicine, Tsukuba Medical Center Hospital, Ibaraki, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yamakawa
authors.affiliation: Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Maiko
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Miroku Medical Laboratory Co., Ltd., Nagano, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yaguchi
authors.affiliation: Miroku Medical Laboratory Co., Ltd., Nagano, Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Notake
authors.affiliation: Miroku Medical Laboratory Co., Ltd., Nagano, Japan.
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Tamai
authors.affiliation: Miroku Medical Laboratory Co., Ltd., Nagano, Japan.
authors.forename: Hideji
authors.initials: H
authors.lastname: Yanagisawa
authors.affiliation: Department of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Misawa
authors.affiliation: Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yanagihara
mesh_terms: D057205:Automation, Laboratory / Q000379:methods; D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial
keywords: Bacteremia; Blood culture; Gram-negative bacteria; Nucleic acid testing; Verigene