title: Highly Sensitive and Selective Sensor Chips with Graphene-Oxide Linking Layer.
abstract: The development of sensing interfaces can significantly improve the performance of biological sensors. Graphene oxide provides a remarkable immobilization platform for surface plasmon resonance (SPR) biosensors due to its excellent optical and biochemical properties. Here, we describe a novel sensor chip for SPR biosensors based on graphene-oxide linking layers. The biosensing assay model was based on a graphene oxide film containing streptavidin. The proposed sensor chip has three times higher sensitivity than the carboxymethylated dextran surface of a commercial sensor chip. Moreover, the demonstrated sensor chips are bioselective with more than 25 times reduced binding for nonspecific interaction and can be used multiple times. We consider the results presented here of importance for any future applications of highly sensitive SPR biosensing.
authors.affiliation: Laboratory of Nanooptics and Plasmonics, Moscow Institute of Physics and Technology , 9 Institutsky Lane, Dolgoprudny 141700, Russian Federation.
authors.forename: Yury V
authors.initials: YV
authors.lastname: Stebunov
authors.affiliation: Laboratory of Nanooptics and Plasmonics, Moscow Institute of Physics and Technology , 9 Institutsky Lane, Dolgoprudny 141700, Russian Federation.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Aftenieva
authors.affiliation: Laboratory of Nanooptics and Plasmonics, Moscow Institute of Physics and Technology , 9 Institutsky Lane, Dolgoprudny 141700, Russian Federation.
authors.forename: Aleksey V
authors.initials: AV
authors.lastname: Arsenin
authors.affiliation: Laboratory of Nanooptics and Plasmonics, Moscow Institute of Physics and Technology , 9 Institutsky Lane, Dolgoprudny 141700, Russian Federation.|Institute of Technology and Innovation, University of Southern Denmark , Campusvej 55, DK-5230 Odense M, Denmark.
authors.forename: Valentyn S
authors.initials: VS
authors.lastname: Volkov
mesh_terms: D000327:Adsorption; D004867:Equipment Design; D006108:Graphite / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
chemical_list: D010087:Oxides; D006108:Graphite
keywords: graphene; graphene oxide; sensing interface; sensor chips; surface plasmon resonance