title: Terahertz spectroscopy of oligonucleotides in aqueous solutions.
abstract: A terahertz (THz) spectroscopic study is carried out to analyze DNA mutations in a label-free manner. Three newly designed liquid sample cells are considered and the best is selected as the sample carrier for THz transmission spectroscopic analyses. Discrimination based on spectral signatures of single-base mutations on single-stranded 20 nt oligonucleotides has been shown possible experimentally. The results clearly attest the ability of this promising approach for label-free analyses of single-base mutations of DNA molecules. This study has demonstrated that the THz spectroscopic technology can be considered as a potential diagnostic tool for investigating molecular reactions, such as DNA mutations.
authors.affiliation: Chinese Academy of Sciences, Chongqing Institute of Green and Intelligent Technology, Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, No. 266 Fangzheng Avenue, Shuitu Hi-tech Industrial Park, Shuitu Town, Beibei District, Chongqing 40071.
authors.forename: Mingjie
authors.initials: M
authors.lastname: Tang
authors.affiliation: Third Military Medical University, Southwest Hospital, Department of Laboratory Medicine, Chongqing 400038, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Chinese Academy of Sciences, Chongqing Institute of Green and Intelligent Technology, Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, No. 266 Fangzheng Avenue, Shuitu Hi-tech Industrial Park, Shuitu Town, Beibei District, Chongqing 40071.
authors.forename: Dongshan
authors.initials: D
authors.lastname: Wei
authors.affiliation: Capital Normal University, Department of Physics, Beijing 100048, China.
authors.forename: Guozhong
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Chinese Academy of Sciences, Chongqing Institute of Green and Intelligent Technology, Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, No. 266 Fangzheng Avenue, Shuitu Hi-tech Industrial Park, Shuitu Town, Beibei District, Chongqing 40071.
authors.forename: Tianying
authors.initials: T
authors.lastname: Chang
authors.affiliation: Capital Normal University, Department of Physics, Beijing 100048, China.
authors.forename: Kuan
authors.initials: K
authors.lastname: Kou
authors.affiliation: Jilin University, College of Instrumentation Science and Electrical Engineering, Changchun, Jilin 130061, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Chinese Academy of Sciences, Chongqing Institute of Green and Intelligent Technology, Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, No. 266 Fangzheng Avenue, Shuitu Hi-tech Industrial Park, Shuitu Town, Beibei District, Chongqing 40071.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Du
authors.affiliation: Third Military Medical University, Southwest Hospital, Department of Laboratory Medicine, Chongqing 400038, China.
authors.forename: Wei-ling
authors.initials: WL
authors.lastname: Fu
authors.affiliation: Chinese Academy of Sciences, Chongqing Institute of Green and Intelligent Technology, Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, No. 266 Fangzheng Avenue, Shuitu Hi-tech Industrial Park, Shuitu Town, Beibei District, Chongqing 40071.
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Cui
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000295:instrumentation / Q000379:methods*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*; D012996:Solutions; D055068:Terahertz Spectroscopy / Q000295:instrumentation / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: D012996:Solutions; D014867:Water; D004247:DNA