title: MiR-34a is Involved in the Decrease of ATP Contents Induced by Resistin Through Target on ATP5S in HepG2 Cells.
abstract: Resistin is associated with metabolic syndrome and deciphering its developmental and molecular mechanisms may help the development of new treatments. MiRNAs serve as negative regulators in many physiological and pathological processes. Here, miRNA microarrays were used to detect differences in expression between resistin-treated and control mice, and results showed miR-34a to be upregulated by resistin. The purpose of this study was to determine whether miR-34a played a role in resistin-induced decrease of ATP contents. Transient transfection of miR-34a mimics was used to overexpress miR-34a and quantitative RT-PCR was used to detect its expression. Western blot analysis was used to determine the rate of expression at the protein level. ATP content was measured using an ATP assay kit. The target gene of miR-34a was analyzed using bioinformatics and confirmed with dual-luciferase report system. MiR-34a was upregulated by resistin in HepG2 cells, and overexpression of miR-34a was found to diminish ATP levels significantly. This study is the first to show that ATP5S is one of the target genes of miR-34a. Resistin diminishes ATP content through the targeting of ATP5S mRNA 3'UTR by miR-34a.
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China. newwfyun@163.com.
authors.forename: Fengyun
authors.initials: F
authors.lastname: Wen
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Wei
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China.
authors.forename: Jianyu
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China.
authors.forename: Haiwei
authors.initials: H
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D008647:Mesocricetus; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D046228:Microarray Analysis; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D052243:Resistin / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; C499624:RETN protein, human; D012333:RNA, Messenger; D052243:Resistin; D000255:Adenosine Triphosphate; C000608097:DMAC2L protein, human; D025261:Mitochondrial Proton-Translocating ATPases
keywords: ATP contents; ATP5S; Resistin; miR-34a