title: Down-regulation of miR-326 is associated with poor prognosis and promotes growth and metastasis by targeting FSCN1 in gastric cancer.
abstract: BACKGROUND
  MicroRNAs (miRNAs) have been documented as playing important roles in diverse biological processes including tumorigenesis. However, the function and mechanism of miR-326 in gastric cancer are still unknown. The aim of this study is to identify the role of miR-326 in gastric cancer and clarify the regulation of Fascin1 (FSCN1) by miR-326.
  
  
  METHODS
  The expression levels of miR-326 were detected in gastric cancer samples and cell lines by real-time PCR. The clinical and prognostic significance of miR-326 in gastric cancer patients were analyzed. Furthermore, the function of miR-326 on tumor cell growth and mobility were explored through MTT, colony formation, Transwell migration and invasion assays in vitro. A miR-326 target was confirmed using luciferase reporter assays, real-time PCR and Western blot.
  
  
  RESULTS
  Our study showed that miR-326 expression was decreased in gastric cancer tissues and cell lines, and low expression of miR-326 was associated to clinical stage, tumor depth, lymph node metastasis and distant metastasis. In survival analysis, low expression of miR-326 was a poor independent prognostic factor for gastric cancer patients. Gain-of-function and loss-of-function studies showed that miR-326 served as a tumor suppressor regulating gastric cancer cells growth, migration and invasion. Furthermore, we identified FSCN1 as the functional target of miR-326 by directly targeting the 3'-UTR of FSCN1.
  
  
  CONCLUSIONS
  Our study demonstrated that miR-326 overexpression was a poor prognostic marker for gastric cancer patients, and miR-326 served as a tumor suppressor in gastric cancer via directly regulating FSCN1.
authors.affiliation: a Department of Gastroenterology Surgery and.
authors.forename: Yanliang
authors.initials: Y
authors.lastname: Li
authors.affiliation: b Department of Pathology , Shandong Provincial Cancer Hospital , Jinan , Shandong , China .
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Gao
authors.affiliation: c Intensive Care Unit, Wucheng People's Hospital , Wucheng , Shandong , China , and.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xu
authors.affiliation: a Department of Gastroenterology Surgery and.
authors.forename: Heng
authors.initials: H
authors.lastname: Ma
authors.affiliation: d Department of Urology , Shandong Provincial Cancer Hospital , Jinan , Shandong , China.
authors.forename: Mingshan
authors.initials: M
authors.lastname: Yang
mesh_terms: D020413:3' Untranslated Regions; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002465:Cell Movement; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009362:Neoplasm Metastasis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; D002352:Carrier Proteins; C502658:FSCN1 protein, human; C545322:MIRN326 microRNA, human; D035683:MicroRNAs; D008840:Microfilament Proteins
keywords: FSCN1; gastric cancer; miR-326; prognosis