title: Role of amino acids as additives on sperm motility, plasma membrane integrity and lipid peroxidation levels at pre-freeze and post-thawed ram semen.
abstract: The possibility of including amino acids for cryopreservation of ram semen to improve the quality of frozen semen was explored in this study in sheep model. 24 samples were collected in triplicate from 8 rams of 2-3 year old Bannur cross bred rams maintained at the Institute Experimental Livestock Unit. Semen was diluted in tris-egg yolk glycerol diluent and made into 7 aliquots as follows: aliquot 1 served as control, "l-alanine" was added at 100 and 135mM in the aliquots 2 and 3, "l-glutamine" was added at 20 and 25mM in the aliquots 4 and 5 and "l-proline" was added at 25 and 50mM in the aliquots 6 and 7, respectively. Diluted semen was filled in 0.25ml French straws and frozen in LN2. Inclusion of "l-proline" and "l-glutamine" in the diluent increased the percent live sperm (P<0.001), total motility (P<0.05) and maintained higher functional membrane and acrosomal integrity (P<0.001) by decreasing lipid peroxidation (P<0.001) compared to the control group. In contrast, "l-alanine" decreased the percentage of total motility, fast progressive spermatozoa and increased (P<0.01) the percentage of immotile spermatozoa. It can be concluded that 20mM "l-glutamine" and 25mM "l-proline" can be used as semen additive to freeze ram semen as they prevented cryoinjuries to sperm and improved the pre-freeze and post-thaw semen characteristics.
authors.affiliation: Veterinary College, KVAFSU, Nandinagar, Bidar 585401, India.
authors.forename: Sharon
authors.initials: S
authors.lastname: Sangeeta
authors.affiliation: ICAR-National Institute of Animal Nutrition and Physiology, Bangalore 560030, India. Electronic address: arangasamyars@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Arangasamy
authors.affiliation: Veterinary College, KVAFSU, Nandinagar, Bidar 585401, India.
authors.forename: S
authors.initials: S
authors.lastname: Kulkarni
authors.affiliation: ICAR-National Institute of Animal Nutrition and Physiology, Bangalore 560030, India.
authors.forename: S
authors.initials: S
authors.lastname: Selvaraju
mesh_terms: D000409:Alanine / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects* / Q000502:physiology; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D005973:Glutamine / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D011392:Proline / Q000494:pharmacology; D012661:Semen / Q000187:drug effects* / Q000502:physiology; D012756:Sheep; D013081:Sperm Motility / Q000187:drug effects* / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology
chemical_list: D000596:Amino Acids; D005973:Glutamine; D011392:Proline; D000409:Alanine
keywords: Amino acids; Freezing; HOS-G; Lipid peroxidation; Ram semen