title: Comparative analysis of complete chloroplast genome sequences of two tropical trees Machilus yunnanensis and Machilus balansae in the family Lauraceae.
abstract: Machilus is a large (c. 100 sp.) genus of trees in the family Lauraceae, distributed in tropical and subtropical East Asia. Both molecular species identification and phylogenetic studies of this morphologically uniform genus have been constrained by insufficient variable sites among frequently used biomarkers. To better understand the mutation patterns in the chloroplast genome of Machilus, the complete plastomes of two species were sequenced. The plastomes of Machilus yunnanensis and M. balansae were 152, 622 and 152, 721 bp, respectively. Seven highly variable regions between the two Machilus species were identified and 297 mutation events, including one micro-inversion in the ccsA-ndhD region, 65 indels, and 231 substitutions, were accurately located. Thirty-six microsatellite sites were found for use in species identification and 95 single-nucleotide changes were identified in gene coding regions.
authors.affiliation: Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences Mengla, China ; Graduate School of the Chinese Academy of Sciences Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Song
authors.affiliation: Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University Beijing, China ; State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences Beijing, China.
authors.forename: Wenpan
authors.initials: W
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences Beijing, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences Beijing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xu
authors.affiliation: Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences Mengla, China ; Graduate School of the Chinese Academy of Sciences Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yao
authors.affiliation: Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences Mengla, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences Mengla, China.
authors.forename: Richard T
authors.initials: RT
authors.lastname: Corlett
keywords: Lauraceae; Machilus; chloroplast; genome; mutation