title: Oxidative stress, DNA damage and antioxidant enzyme activities in the pacific white shrimp (Litopenaeus vannamei) when exposed to hypoxia and reoxygenation.
abstract: To evaluate the genotoxic and physiological effects of acute hypoxia on the pacific white shrimp (L. vannamei), shrimps were treated firstly with three dissolved oxygen levels 6.5 ppm (control), 3.0 ppm and 1.5 ppm for 24 h, respectively, and then reoxygenated (6.5 ppm) for 24 h. The changes of superoxide dismutase (SOD) activity, glutathione peroxidases (GPX) activity, malondialdehyde (MDA) concentration and DNA damage in the tissues of gill, hepatopancreas and hemolymph were examined during the period of hypoxia and reoxygenation. The results indicated SOD activity, GPX activity, MDA concentration and DNA damage all increased basically compared with the control during the period of hypoxia except for MDA concentrations in the gill at 12 h and 24 h hypoxia (3.0 ppm), and these parameters were recovered to some degree during the period of reoxygenation. Moreover, the comet assays in the tissues of gill and hepatopancreas showed an obvious time- and dose-dependent response to hypoxia, which indicated comet assay in the two tissues could be used as sensitive biomarker to detect the occurrence of hypoxia. We conclude that acute hypoxia can induce oxidative stress, DNA damage and lipid peroxidation in the tissues of gill, hepatopancreas and hemolymph of L. vannamei and the DNA damage may come from hypoxia-induced oxidative stress.
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, China; Haikou Key Laboratory of Environment Toxicology, Haikou 570228, China; Key Laboratory of Tropical Biological Resources, Ministry of Education, Hainan University, Haikou 570228, China.
authors.forename: Yuhu
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wei
authors.affiliation: Hainan Provincial Health School, Haikou 570311, China.
authors.forename: Jiangrong
authors.initials: J
authors.lastname: Cao
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, China.
authors.forename: Liguo
authors.initials: L
authors.lastname: Qiu
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Jiang
authors.affiliation: College of Plant Protection and Environment, Hainan University, Haikou 570228, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, China.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Song
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, China. Electronic address: zhouhl@hainu.edu.cn.
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Han
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, China. Electronic address: diaoxip@hainu.edu.cn.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Diao
mesh_terms: D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005880:Gills / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D006458:Hemolymph / Q000201:enzymology / Q000378:metabolism; D043143:Hepatopancreas / Q000201:enzymology / Q000378:metabolism; D000860:Hypoxia / Q000235:genetics / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects / Q000235:genetics; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D010100:Oxygen / Q000378:metabolism* / Q000494:pharmacology; D033561:Penaeidae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
chemical_list: D008315:Malondialdehyde; D013482:Superoxide Dismutase; D010100:Oxygen
keywords: DNA damage; Hypoxia; Litopenaeus vannamei; Oxidative stress; Reoxygenation