title: Oxidative stress and damage to erythrocytes in patients with chronic obstructive pulmonary disease--changes in ATPase and acetylcholinesterase activity.
abstract: The study indicates, for the first time, the changes in both ATPase and AChE activities in the membrane of red blood cells of patients diagnosed with COPD. Chronic obstructive pulmonary disease (COPD) is one of the most common and severe lung disorders. We examined the impact of COPD on redox balance and properties of the membrane of red blood cells. The study involved 30 patients with COPD and 18 healthy subjects. An increase in lipid peroxidation products and a decrease in the content of -SH groups in the membrane of red blood cells in patients with COPD were observed. Moreover, an increase in the activity of glutathione peroxidase and a decrease in superoxide dismutase, but not in catalase activity, were found as well. Significant changes in activities of erythrocyte membrane enzymes in COPD patients were also evident demonstrated by a considerably lowered ATPase activity and elevated AChE activity. Changes in the structure and function of red blood cells observed in COPD patients, together with changes in the activity of the key membrane enzymes (ATPases and AChE), can result from the imbalance of redox status of these cells due to extensive oxidative stress induced by COPD disease.
authors.affiliation: a Department of Environmental Pollution Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Bożena
authors.initials: B
authors.lastname: Bukowska
authors.affiliation: a Department of Environmental Pollution Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Sicińska
authors.affiliation: c Department of Pneumology and Allergology of Norbert Barlicki Memorial University Hospital, Medical University of Lodz, Kopcińskiego 22, 90-153 Łódź, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Pająk
authors.affiliation: b Department of Thermobiology, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Koceva-Chyla
authors.affiliation: c Department of Pneumology and Allergology of Norbert Barlicki Memorial University Hospital, Medical University of Lodz, Kopcińskiego 22, 90-153 Łódź, Poland.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Pietras
authors.affiliation: a Department of Environmental Pollution Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Pszczółkowska
authors.affiliation: c Department of Pneumology and Allergology of Norbert Barlicki Memorial University Hospital, Medical University of Lodz, Kopcińskiego 22, 90-153 Łódź, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Górski
authors.affiliation: a Department of Environmental Pollution Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Koter-Michalak
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000097:blood / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D015536:Down-Regulation; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000473:pathology; D004912:Erythrocytes / Q000201:enzymology* / Q000473:pathology; D058851:GPI-Linked Proteins / Q000097:blood / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D012720:Severity of Illness Index; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000097:blood / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D058851:GPI-Linked Proteins; D013438:Sulfhydryl Compounds; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C548244:ACHE protein, human; D000110:Acetylcholinesterase; D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase
keywords: ATPase; acetylcholinesterase; acétylcholinestérase; chronic obstructive pulmonary disease; erythrocytes; maladie pulmonaire obstructive chronique; oxidative stress; stress oxydant; superoxide dismutase; superoxyde dismutase; érythrocytes