title: Antibody-reactive class I epitopes defined by pairs of mismatched eplets and self-eplets.
abstract: The identification of human leukocyte antigen (HLA) antibodies in the sera of candidates awaiting organ transplantation has evolved over time. This has been possible because of the introduction of more sensitive techniques and to the increasing focus on the structural aspects of the HLA epitopes. The use of the HLAMatchmaker algorithm in the analysis of positive sera and the verification of HLA ABC epitopes in the HLA Epitope Registry website provide new stimuli on the interpretation of antibody reactivity. The epitopes defined by eplet pairs often involve a nonself-eplet and a self-eplet (nonself-self paradigm), suggesting that the antibody response to an HLA mismatch must have an auto-reactive component. Here, we report an application of the nonself-self paradigm that provides a basis for better knowledge and interpretation of HLA-antibody reactivity in Luminex assays with single alleles.
authors.affiliation: U.O.C. Immunohematology, Transfusion Medicine and Transplant Immunology (SIMT), Regional Reference Laboratory of Transplant Immunology (LIT), Azienda Ospedaliera Universitaria (AOU), Second University of Naples, Naples, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Resse
authors.affiliation: U.O.C. Immunohematology, Transfusion Medicine and Transplant Immunology (SIMT), Regional Reference Laboratory of Transplant Immunology (LIT), Azienda Ospedaliera Universitaria (AOU), Second University of Naples, Naples, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Paolillo
authors.affiliation: U.O.C. Immunohematology, Transfusion Medicine and Transplant Immunology (SIMT), Regional Reference Laboratory of Transplant Immunology (LIT), Azienda Ospedaliera Universitaria (AOU), Second University of Naples, Naples, Italy.
authors.forename: B P
authors.initials: BP
authors.lastname: Minucci
authors.affiliation: U.O.C. Immunohematology, Transfusion Medicine and Transplant Immunology (SIMT), Regional Reference Laboratory of Transplant Immunology (LIT), Azienda Ospedaliera Universitaria (AOU), Second University of Naples, Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Moccia
authors.affiliation: U.O.C. Immunohematology, Transfusion Medicine and Transplant Immunology (SIMT), Regional Reference Laboratory of Transplant Immunology (LIT), Azienda Ospedaliera Universitaria (AOU), Second University of Naples, Naples, Italy.|Department of Medical, Surgical, Neurological, Metabolic and Geriatric Sciences, Second University of Naples, Naples, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Napoli
mesh_terms: D000465:Algorithms; D000906:Antibodies / Q000737:chemistry* / Q000235:genetics; D000939:Epitopes / Q000737:chemistry* / Q000235:genetics; D006680:HLA Antigens / Q000737:chemistry* / Q000235:genetics; D006801:Humans
chemical_list: D000906:Antibodies; D000939:Epitopes; D006680:HLA Antigens
keywords: HLAMatchmaker; eplet pairs; human leukocyte antigen antibodies; nonself-self paradigm