title: Efficient Activation of Pathogenic ΔPhe501 Mutation in Monocarboxylate Transporter 8 by Chemical and Pharmacological Chaperones.
abstract: Monocarboxylate transporter 8 (MCT8) is a thyroid hormone transmembrane transporter expressed in many cell types, including neurons. Mutations that inactivate transport activity of MCT8 cause severe X-linked psychomotor retardation in male patients, a syndrome originally described as the Allan-Herndon-Dudley syndrome. Treatment options currently explored the focus on finding thyroid hormone-like compounds that bypass MCT8 and enter cells through different transporters. Because MCT8 is a multipass transmembrane protein, some pathogenic mutations affect membrane trafficking while potentially retaining some transporter activity. We explore here the effects of chemical and pharmacological chaperones on the expression and transport activity of the MCT8 mutant ΔPhe501. Dimethylsulfoxide, 4-phenylbutyric acid as well as its sodium salt, and the isoflavone genistein increase T3 uptake into MDCK1 cells stably transfected with mutant MCT8-ΔPhe501. We show that ΔPhe501 represents a temperature-sensitive mutant protein that is stabilized by the proteasome inhibitor MG132. 4-Phenylbutyrate has been used to stabilize ΔPhe508 mutant cystic fibrosis transmembrane conductance regulator protein and is in clinical use in patients with urea cycle defects. Genistein is enriched in soy and available as a nutritional supplement. It is effective in stabilizing MCT8-ΔPhe501 at 100 nM concentration. Expression of the L471P mutant is increased in response to phenylbutyrate, but T3 uptake activity is not induced, supporting the notion that the chaperone specifically increases membrane expression. Our findings suggest that certain pathogenic MCT8 mutants may be responsive to (co-)treatment with readily available compounds, which increase endogenous protein function.
authors.affiliation: Institut für Biochemie und Molekularbiologie, Rheinische Friedrich-Wilhelms-Universität, 53115 Bonn, Germany.
authors.forename: Doreen
authors.initials: D
authors.lastname: Braun
authors.affiliation: Institut für Biochemie und Molekularbiologie, Rheinische Friedrich-Wilhelms-Universität, 53115 Bonn, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schweizer
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004285:Dogs; D019833:Genistein / Q000494:pharmacology; D007457:Iodine Radioisotopes; D007976:Leupeptins / Q000494:pharmacology; D061985:Madin Darby Canine Kidney Cells; D038901:X-Linked Intellectual Disability; D018613:Microscopy, Confocal; D027501:Monocarboxylic Acid Transporters / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D009123:Muscle Hypotonia; D009133:Muscular Atrophy; D009154:Mutation; D009865:Oocytes / Q000378:metabolism; D010654:Phenylbutyrates / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects*; D027981:Symporters; D013974:Thyroxine / Q000378:metabolism*; D014284:Triiodothyronine / Q000378:metabolism*; D014981:Xenopus
chemical_list: D015853:Cysteine Proteinase Inhibitors; D007457:Iodine Radioisotopes; D007976:Leupeptins; D027501:Monocarboxylic Acid Transporters; D010654:Phenylbutyrates; C090735:SLC16A2 protein, human; D027981:Symporters; D014284:Triiodothyronine; C075773:4-phenylbutyric acid; D019833:Genistein; D013974:Thyroxine; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde; D004121:Dimethyl Sulfoxide