title: Protein-based photothermal theranostics for imaging-guided cancer therapy.
abstract: The development of imageable photothermal theranostics has attracted considerable attention for imaging guided photothermal therapy (PTT) with high tumor ablation accuracy. In this study, we strategically constructed a near-infrared (NIR) cyanine dye by introducing a rigid cyclohexenyl ring to the heptamethine chain to obtain a heptamethine dye CySCOOH with high fluorescence intensity and good stability. By covalent conjugation of CySCOOH onto human serum albumin (HSA), the as-prepared HSA@CySCOOH nanoplatform is highly efficient for NIR fluorescence/photoacoustic/thermal multimodality imaging and photothermal tumor ablation. The theranostic capability of HSA@CySCOOH was systematically evaluated both in vitro and in vivo. Most intriguingly, complete tumor elimination was achieved by intravenous injection of HSA@CySCOOH (CySCOOH, 1 mg kg(-1); 808 nm, 1.0 W cm(-2) for 5 min) into 4T1 tumor-bearing mice, with no weight loss, noticeable toxicity, or tumor recurrence being observed. This as-prepared protein-based nanotheranostics exhibits high water dispersibility, no off target cytotoxicity, and good biodegradability and biocompatibility, thus facilitating its clinical translation to cancer photothermal theranostics.
authors.affiliation: Department of Radiology, the Third Xiangya Hospital, Central South University, Changsha, 410013, PR China. cjr.wangwei@vip.163.com.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Rong
authors.forename: Peng
authors.initials: P
authors.lastname: Huang
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Albert
authors.initials: A
authors.lastname: Jin
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.forename: Gang
authors.initials: G
authors.lastname: Niu
authors.forename: Lun
authors.initials: L
authors.lastname: Yu
authors.forename: Wenbin
authors.initials: W
authors.lastname: Zeng
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D061986:MCF-7 Cells; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D061848:Optical Imaging / Q000379:methods*; D010778:Photochemotherapy / Q000379:methods*; D012709:Serum Albumin / Q000737:chemistry / Q000494:pharmacology
chemical_list: D005456:Fluorescent Dyes; D012709:Serum Albumin