title: Houttuyniae Herba Attenuates Kainic Acid-Induced Neurotoxicity via Calcium Response Modulation in the Mouse Hippocampus.
abstract: Epilepsy is a complex neurological disorder characterized by the repeated occurrence of electrical activity known as seizures. This activity induces increased intracellular calcium, which ultimately leads to neuronal damage. Houttuyniae Herba, the aerial part of Houttuynia cordata, has various pharmacological effects and is widely used as a traditional herb. In the present study, we evaluated the protective effects of Houttuyniae Herba water extract on kainic acid-induced neurotoxicity. Kainic acid directly acts on calcium release, resulting in seizure behavior, neuronal damage, and cognitive impairment. In a rat primary hippocampal culture system, Houttuyniae Herba water extract significantly protected neuronal cells from kainic acid toxicity. In a seizure model where mice received intracerebellar kainic acid injections, Houttuyniae Herba water extract treatment resulted in a lower seizure stage score, ameliorated cognitive impairment, protected neuronal cells against kainic acid-induced toxicity, and suppressed neuronal degeneration in the hippocampus. In addition, Houttuyniae Herba water extract regulated increases in the intracellular calcium level, its related downstream pathways (reactive oxygen species production and mitochondrial dysfunction), and calcium/calmodulin complex kinase type II immunoreactivity in the mouse hippocampus, which resulted from calcium influx stimulation induced by kainic acid. These results demonstrate the neuroprotective effects of Houttuyniae Herba water extract through inhibition of calcium generation in a kainic acid-induced epileptic model.
authors.affiliation: Department of Life and Nanopharmaceutical Science, Graduate School, Kyung Hee East-West Pharmaceutical Research Institute, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Hyo Geun
authors.initials: HG
authors.lastname: Kim
authors.affiliation: Department of Life and Nanopharmaceutical Science, Graduate School, Kyung Hee East-West Pharmaceutical Research Institute, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Hyun Uk
authors.initials: HU
authors.lastname: Jeong
authors.affiliation: Department of East-West Integrated Medicine, College of Korean Medicine, Gachon University, Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: Sung In
authors.initials: SI
authors.lastname: Hong
authors.affiliation: Department of Life and Nanopharmaceutical Science, Graduate School, Kyung Hee East-West Pharmaceutical Research Institute, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Myung Sook
authors.initials: MS
authors.lastname: Oh
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D052859:Houttuynia / Q000737:chemistry*; D007608:Kainic Acid / Q000037:antagonists & inhibitors; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009498:Neurotoxins / Q000037:antagonists & inhibitors; D051381:Rats; D012640:Seizures / Q000139:chemically induced / Q000517:prevention & control*
chemical_list: D018696:Neuroprotective Agents; D009498:Neurotoxins; D007608:Kainic Acid; D002118:Calcium