title: Gallic acid induces mitotic catastrophe and inhibits centrosomal clustering in HeLa cells.
abstract: Cancer cells divide rapidly, providing medical targets for anticancer agents. The polyphenolic gallic acid (GA) is known to be toxic for certain cancer cells. However, the cellular mode of action has not been elucidated. Therefore, the current study addressed a potential effect of GA on the mitosis of cancer cells. GA inhibited viability of HeLa cells in a dose-dependent and time-dependent manner. We could show, using fluorescence-activated cell sorting (FACS), that this inhibition was accompanied by elevated frequency of cells arrested at the G2/M transition. This cell-cycle arrest was accompanied by mitotic catastrophe, and formation of cells with multiple nuclei. These aberrations were preceded by impaired centrosomal clustering. We arrive at a model of action, where GA inhibits the progression of the cell cycle at the G2/M phase by impairing centrosomal clustering which will stimulate mitotic catastrophe. Thus, GA has potential as compound against cervical cancer.
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China; Molecular Cell Biology, Botanical Institute 1, Karlsruhe Institute of Technology, Karlsruhe 76131, Germany; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen 76344, Germany.
authors.forename: Si
authors.initials: S
authors.lastname: Tan
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Guan
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen 76344, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Grün
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China. Electronic address: zzqswu@yahoo.com.
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen 76344, Germany. Electronic address: ute.schepers@kit.edu.
authors.forename: Ute
authors.initials: U
authors.lastname: Schepers
authors.affiliation: Molecular Cell Biology, Botanical Institute 1, Karlsruhe Institute of Technology, Karlsruhe 76131, Germany. Electronic address: peter.nick@kit.edu.
authors.forename: Peter
authors.initials: P
authors.lastname: Nick
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018385:Centrosome / Q000187:drug effects*; D005707:Gallic Acid / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000187:drug effects*; D014404:Tubulin / Q000737:chemistry
chemical_list: D014404:Tubulin; D005707:Gallic Acid
keywords: Cell-cycle arrest; Centrosome clustering; Gallic acid (GA); HeLa; Mitotic catastrophe