title: Bleomycin enhances the efficacy of sonodynamic therapy using aluminum phthalocyanine disulfonate.
abstract: Sonodynamic therapy (SDT), or ultrasound combined with sonosensitization, is a promising approach because it is noninvasive and penetrates deeper than light does in photodynamic therapy. We examined whether bleomycin (BLM) could improve the efficacy of SDT. We performed an in vitro study using Colon-26 cells, which are derived from mouse colon cancer. SDT with BLM was significantly more cytotoxic than SDT alone both in vitro and in vivo. We also observed an ultrasound intensity-dependent cytotoxic effect of SDT with BLM. These findings suggest that SDT with BLM might provide a novel noninvasive treatment for deep-seated tumors.
authors.affiliation: Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, Tottori, Japan. Electronic address: tosaki@muses.tottori-u.ac.jp.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Osaki
authors.affiliation: Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, Tottori, Japan.
authors.forename: Inoru
authors.initials: I
authors.lastname: Yokoe
authors.affiliation: Department of Life System, Institute of Technology and Science, Graduate School, The University of Tokushima, Tokushima, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Uto
authors.affiliation: SBI Pharmaceuticals Co., Ltd., Tokyo, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ishizuka
authors.affiliation: SBI Pharmaceuticals Co., Ltd., Tokyo, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: ITO Physiotherapy & Rehabilitation, Tokyo, Japan.
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Yamanaka
authors.affiliation: ITO Physiotherapy & Rehabilitation, Tokyo, Japan.
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Kurahashi
authors.affiliation: Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, Tottori, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Azuma
authors.affiliation: Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, Tottori, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Murahata
authors.affiliation: Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, Tottori, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tsuka
authors.affiliation: Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, Tottori, Japan.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Ito
authors.affiliation: Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, Tottori, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imagawa
authors.affiliation: Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, Tottori, Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Okamoto
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D007211:Indoles / Q000494:pharmacology*; D051379:Mice; D009942:Organometallic Compounds / Q000494:pharmacology*; D013997:Time Factors; D000069453:Ultrasonic Waves
chemical_list: D007211:Indoles; D009942:Organometallic Compounds; D001761:Bleomycin; C064331:aluminum phthalocyanine disulfonate; D053148:Caspase 3; D053179:Caspase 7
keywords: Bleomycin; Disulfonated aluminum phthalocyanine; Sonodynamic therapy; Ultrasound