title: Classification of illicit heroin by UPLC-Q-TOF analysis of acidic and neutral manufacturing impurities.
abstract: The illicit manufacture of heroin results in the formation of trace levels of acidic and neutral manufacturing impurities that provide valuable information about the manufacturing process used. In this work, a new ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF) method; that features high resolution, mass accuracy and sensitivity for profiling neutral and acidic heroin manufacturing impurities was developed. After the UPLC-Q-TOF analysis, the retention times and m/z data pairs of acidic and neutral manufacturing impurities were detected, and 19 peaks were found to be evidently different between heroin samples from "Golden Triangle" and "Golden Crescent". Based on the data set of these 19 impurities in 150 authentic heroin samples, classification of heroin geographic origins was successfully achieved utilizing partial least squares discriminant analysis (PLS-DA). By analyzing another data set of 267 authentic heroin samples, the developed discrimiant model was validated and proved to be accurate and reliable.
authors.affiliation: National Narcotic Laboratory, Drug Intelligence and Forensic Center of Minister of Public Security, Beijing, China.
authors.forename: Cuimei
authors.initials: C
authors.lastname: Liu
authors.affiliation: National Narcotic Laboratory, Drug Intelligence and Forensic Center of Minister of Public Security, Beijing, China. Electronic address: zdhuapku@163.com.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Hua
authors.affiliation: National Narcotic Laboratory, Drug Intelligence and Forensic Center of Minister of Public Security, Beijing, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Bai
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D016002:Discriminant Analysis; D004340:Drug Contamination; D003932:Heroin / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013287:Illicit Drugs / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D013287:Illicit Drugs; D003932:Heroin
keywords: Acidic and neutral manufacturing impurities; Heroin; Origin classification; PLS-DA; UPLC–Q-TOF