title: Cyanoacrylate medical glue application in intervertebral disc annulus defect repair: Mechanical and biocompatible evaluation.
abstract: In an attempt to find an ideal closure method during annulus defect repair, we evaluate the use of medical glue by mechanical and biocompatible test. Cyanoacrylate medical glue was applied together with a multilayer microfiber/nanofiber polycaprolactone scaffold and suture in annulus repair. Continuous axial loading and fatigue mechanical test was performed. Furthermore, the in vitro response of mesenchymal stem cell (MSC) to the glue was evaluated by cell viability assay. The in vivo response of annulus tissue to the glue and scaffold was also studied in porcine lumbar spine; histological sections were evaluated after 3 months. Cyanoacrylate glue significantly improved the closure effect in the experimental group with failure load 2825.7 ± 941.6 N, compared to 774.1 ± 281.3 N in the control group without glue application (p < 0.01). The experimental group also withstood the fatigue test. No toxic effect was observed by in vitro cell culture and in vivo implantation. On the basis of this initial evaluation, the use of cyanoacrylate medical glue improves closure effect with no toxicity in annulus defect repair. This method of annulus repair merits further effectiveness study in vivo. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 14-20, 2017.
authors.affiliation: Orthopaedic Research Lab, Aarhus University Hospital, Aarhus, C 8000, Denmark.|Department of Orthopedic Surgery, Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing, 210028, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Kang
authors.affiliation: Orthopaedic Research Lab, Aarhus University Hospital, Aarhus, C 8000, Denmark.
authors.forename: Haisheng
authors.initials: H
authors.lastname: Li
authors.affiliation: Orthopaedic Research Lab, Aarhus University Hospital, Aarhus, C 8000, Denmark.
authors.forename: Helle
authors.initials: H
authors.lastname: Lysdahl
authors.affiliation: Orthopaedic Research Lab, Aarhus University Hospital, Aarhus, C 8000, Denmark.
authors.forename: Dang
authors.initials: D
authors.lastname: Quang Svend Le
authors.affiliation: Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, C 8000, Denmark.
authors.forename: Menglin
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Orthopedic Surgery, Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing, 210028, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xie
authors.affiliation: Orthopaedic Research Lab, Aarhus University Hospital, Aarhus, C 8000, Denmark.
authors.forename: Cody
authors.initials: C
authors.lastname: Bünger
mesh_terms: D000269:Adhesives / Q000494:pharmacology*; D000818:Animals; D003487:Cyanoacrylates / Q000494:pharmacology*; D007403:Intervertebral Disc / Q000293:injuries / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D013552:Swine
chemical_list: D000269:Adhesives; D003487:Cyanoacrylates
keywords: annulus repair; biocompatible test; cyanoacrylate glue; mechanical test; scaffold