title: Enzymatic synthesis of novel isobavachalcone glucosides via a UDP-glycosyltransferase.
abstract: Glycosylation is often used to improve a natural product's properties such as water solubility, chemical stability, pharmacological potency, and structure diversification. In this study, we studied the enzymatic synthesis of novel isobavachalcone glucosides using a UDP-glycosyltransferase (YjiC) from Bacillus licheniformis DSM-13. The chemical structures of compounds 1 and 2 were elucidated by spectroscopic techniques, including LC-MS, MS, and NMR. Meanwhile, the parameters of glycosylation reaction such as incubation time, UDP-glucose concentration, and pH of buffer were also optimized during this study. Furthermore, the compounds 1 and 2 exhibited weak anti-proliferative activities against five human cancer cell lines, with IC50 values ranging from 58.6 to 86.6 μM.
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, 2600 Donghai Road, Bengbu, 233030, Anhui, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Li
authors.affiliation: Chemical Biology Research Center, KRIBB, 30 Yeongudanji-ro, Ochang-eup, Chungbuk, Korea.
authors.forename: Jae Kyoung
authors.initials: JK
authors.lastname: Lee
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, 2600 Donghai Road, Bengbu, 233030, Anhui, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Nie
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, 2600 Donghai Road, Bengbu, 233030, Anhui, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huo
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, 2600 Donghai Road, Bengbu, 233030, Anhui, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
authors.affiliation: Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University, Asan, Korea.
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Sohng
authors.affiliation: Chemical Biology Research Center, KRIBB, 30 Yeongudanji-ro, Ochang-eup, Chungbuk, Korea. hongsoo@kribb.re.kr.
authors.forename: Young-Soo
authors.initials: YS
authors.lastname: Hong
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, 2600 Donghai Road, Bengbu, 233030, Anhui, China. wuchengzhu0611@foxmail.com.
authors.forename: Cheng-Zhu
authors.initials: CZ
authors.lastname: Wu
mesh_terms: D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D047188:Chalcones / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D005960:Glucosides / Q000378:metabolism*; D016695:Glycosyltransferases / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D010936:Plant Extracts / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D029907:Psoralea
chemical_list: D047188:Chalcones; D005960:Glucosides; D010936:Plant Extracts; C468754:isobavachalcone; D016695:Glycosyltransferases
keywords: Cytotoxicity; Diversification; Glycosylation; Isobavachalcone; YjiC