title: Differential alterations of tissue T-cell subsets after sepsis.
abstract: Among immune cells in responding to sepsis, macrophages and neutrophils have been extensively studied, while the contribution of T lymphocytes and natural killer T (NKT) cells is less well characterized. Here we monitored tissue specific changes of T cell subsets in male C57BL/6 mice subjected to sham operation or cecal ligation and puncture (CLP) to induce polymicrobial sepsis. Thymus, spleen, liver, lungs and blood were processed and analyzed 20h later. Total lymphocyte count showed a significant reduction in septic thymus, spleen and blood but not in lungs and liver. The septic thymi were hypocellular with severe reduction in cell numbers of immature CD4(+)CD8(+) subset. CD4(+) T and CD8(+) T lymphocyte numbers in septic spleens were also significantly reduced, but the frequency of CD4(+)CD25(+) Tregs was significantly increased. In addition, naïve and Tcm CD4(+) T cell numbers were significantly reduced in the septic spleens. By contrast, in septic liver the CD8(+) T cell numbers were significantly increased, whereas NKT cell numbers were reduced, but more activated with increased CD69 and CD25 expression. In the septic lungs, the CD4(+) T and CD8(+) T cell numbers showed no significant change, whereas they were severely reduced in the septic blood. Overall, this study provides important information on the alterations of different T-cell subsets in various tissues after sepsis.
authors.affiliation: Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, NY 11030, USA. Electronic address: asharma11@nshs.edu.
authors.forename: Archna
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, NY 11030, USA; Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, NY 11030, USA. Electronic address: wlyang@nshs.edu.
authors.forename: Weng-Lang
authors.initials: WL
authors.lastname: Yang
authors.affiliation: Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, NY 11030, USA. Electronic address: Shingo.matsuo123@gmail.com.
authors.forename: Shingo
authors.initials: S
authors.lastname: Matsuo
authors.affiliation: Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, NY 11030, USA; Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, NY 11030, USA. Electronic address: pwang@nshs.edu.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002432:Cecum / Q000293:injuries / Q000601:surgery; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology / Q000378:metabolism; D037181:Lectins, C-Type / Q000276:immunology / Q000378:metabolism; D008026:Ligation / Q000009:adverse effects; D008099:Liver / Q000276:immunology*; D008168:Lung / Q000276:immunology*; D018655:Lymphocyte Count; D008810:Mice, Inbred C57BL; D055611:Natural Killer T-Cells / Q000276:immunology / Q000378:metabolism; D009928:Organ Specificity / Q000276:immunology; D011677:Punctures / Q000009:adverse effects; D018805:Sepsis / Q000097:blood / Q000209:etiology / Q000276:immunology*; D013154:Spleen / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism; D013950:Thymus Gland / Q000276:immunology*; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D016207:Cytokines; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D000099192:CD69 Antigens
keywords: Natural killer T cells; Profiling; Sepsis; T-lymphocytes; Tissue-specificity