title: Determination of C-Terminal δ-Catenin Responsible for Inducing Dendritic Morphogenesis.
abstract: δ-Catenin induces dendritic morphogenesis in several cells and it was reported that deletion of C-terminal 207 amino acid of δ-catenin completely abolished the dendritic morphogenesis. However, exact domain responsible for inducing dendritic morphogenesis in C-terminus of δ-catenin was not mapped. Here, we report that expression of ΔC47 (lacking 47 amino acid of C-terminus: 1-1200), ΔC77 (lacking 77 amino acid of C-terminus: 1-1170) deletion mutants of δ-catenin induced the dendritic morphogenesis of HEK293T and NIH3T3 cells as full-length δ-catenin did. In agreement with previous report, ΔC207 deletion mutant did not show the dendritic morphogenesis of the cells. Interestingly, introducing 107 amino acid deletion of C-terminus (ΔC107 mutant: 1-1140) and 177 amino acid deletion of C-terminus (ΔC177 mutant: 1-1070) showed limited primary and secondary dendritic process and notable spine-like process formation. These results suggest that 1140-1170 amino acid of C-terminal δ-catenin is required for primary and secondary dendrite-like process formation.
authors.forename: Ho-Bin
authors.initials: HB
authors.lastname: Lee
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: He
authors.forename: Gyeong-Su
authors.initials: GS
authors.lastname: Yang
authors.forename: Jin-A
authors.initials: JA
authors.lastname: Oh
authors.forename: Ji-Seon
authors.initials: JS
authors.lastname: Ha
authors.forename: Oh-Hyuen
authors.initials: OH
authors.lastname: Song
authors.forename: Do-Jin
authors.initials: DJ
authors.lastname: Lee
authors.forename: Sang-Chul
authors.initials: SC
authors.lastname: Jung
authors.forename: Kyung Keun
authors.initials: KK
authors.lastname: Kim
authors.forename: Kwonseop
authors.initials: K
authors.lastname: Kim
authors.forename: Hangun
authors.initials: H
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003712:Dendrites / Q000502:physiology* / Q000648:ultrastructure*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D041681:NIH 3T3 Cells; D013329:Structure-Activity Relationship; D051178:alpha Catenin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D051178:alpha Catenin