title: MicroRNA-related single-nucleotide polymorphism of XPO5 is strongly correlated with the prognosis and chemotherapy response in advanced non-small-cell lung cancer patients.
abstract: This study was performed to investigate if the microRNA-related single-nucleotide polymorphisms (miR-SNPs) of XPO5 gene predicted the prognosis and pathological features of advanced non-small-cell lung cancer patients receiving chemotherapy. A total of 131 advanced non-small-cell lung cancer (NSCLC) patients were recruited. MicroRNA (miRNA) binding site prediction software was adopted for the prediction and screening of SNPs in XPO5 and miRNA binding regions. Polymerase chain reaction (PCR) amplification was further performed. Time-dependent survival-free curves were constructed using the Kaplan-Meier technique. Univariate and the multivariate survival analyses were conducted for confirmation of prognostic factor for advanced NSCLC patients receiving chemotherapy. There were no significant differences of SNP distribution frequencies between groups, without statistical significance (P > 0.05). Included clinical pathological features and chemotherapy regimens showed no apparent statistical significance in influencing the curative effect of chemotherapy in advanced NSCLC patients (all P > 0.05). While the objective response rate (ORR) in patients who carried AA and AC genotype was 35.48 and 51.22 %, respectively, with statistically significant difference (P < 0.05). Univariate survival analysis indicated that patients who carried AA genotype showed a significantly lower 5-year survival rate to those who carried AC genotype (P < 0.05). And, considering pathological features, statistical significance was found in patients with different pathological types, lymph node metastasis, differentiation degree, T staging, and pathological staging (all P < 0.05). Multivariate analysis results indicated that the SNP sites of rs11077 might be an independent prognostic factor of advanced NSCLC patients receiving chemotherapy (risk ratio [RR] = 0.346; 95 % confidence interval [95 % CI] = 0.174-0.685, P = 0.002). Other clinical features were all considered to have no apparent effect in influencing the prognostic outcomes of advanced NSCLC patients receiving chemotherapy except lymph node metastasis (P < 0.05). miR-SNP rs11077 of XPO5 may be independently connected with the prognosis and chemotherapy response of advanced NSCLC patients, and patients with AC genotype have relatively improved prognostic outcomes and better curative effect of chemotherapy than those with AA allele of XPO5. Further, lymph node metastasis may be also involved in influencing the prognosis of advanced NSCLC patients.
authors.affiliation: Department of Thoracic and Cardiac Surgery, The Fourth People's Hospital of Wuxi City, Jiangnan University, No. 200, Huihe Road, Wuxi, 214062, People's Republic of China.
authors.forename: Ji-Qun
authors.initials: JQ
authors.lastname: Geng
authors.affiliation: Department of Thoracic and Cardiac Surgery, The Affiliated Jiangyin Hospital of Southwest University Medical College, Jiangyin, 214400, People's Republic of China.|Department of Thoracic and Cardiac Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, People's Republic of China.
authors.forename: Xiao-Chen
authors.initials: XC
authors.lastname: Wang
authors.affiliation: Department of Thoracic and Cardiac Surgery, The Affiliated Xuzhou Hospital of Jiangsu University, Xuzhou, 221000, People's Republic of China.
authors.forename: Long-Fei
authors.initials: LF
authors.lastname: Li
authors.affiliation: Department of Thoracic and Cardiac Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Thoracic and Cardiac Surgery, The Affiliated Jiangyin Hospital of Southwest University Medical College, Jiangyin, 214400, People's Republic of China.
authors.forename: Song
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Thoracic and Cardiac Surgery, The Affiliated Jiangyin Hospital of Southwest University Medical College, Jiangyin, 214400, People's Republic of China.
authors.forename: Gui-Ping
authors.initials: GP
authors.lastname: Yu
authors.affiliation: Department of Thoracic and Cardiac Surgery, The Fourth People's Hospital of Wuxi City, Jiangnan University, No. 200, Huihe Road, Wuxi, 214062, People's Republic of China. zhu714253696@126.com.
authors.forename: Kou-Jun
authors.initials: KJ
authors.lastname: Zhu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D028884:Karyopherins / Q000235:genetics*; D008175:Lung Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D028884:Karyopherins; C446511:XPO5 protein, human
keywords: Advanced non-small-cell lung cancer; MicroRNA-related single-nucleotide polymorphism; Prognosis; XPO5