title: TIA-1 and TIAR interact with 5'-UTR of enterovirus 71 genome and facilitate viral replication.
abstract: Enterovirus 71 is one of the major causative pathogens of HFMD in children. Upon infection, the viral RNA is translated in an IRES-dependent manner and requires several host factors for effective replication. Here, we found that T-cell-restricted intracellular antigen 1 (TIA-1), and TIA-1 related protein (TIAR) were translocated from nucleus to cytoplasm after EV71 infection and localized to the sites of viral replication. We found that TIA-1 and TIAR can facilitate EV71 replication by enhancing the viral genome synthesis in host cells. We demonstrated that both proteins bound to the stem-loop I of 5'-UTR of viral genome and improved the stability of viral genomic RNA. Our results suggest that TIA-1 and TIAR are two new host factors that interact with 5-UTR of EV71 genome and positively regulate viral replication.
authors.affiliation: Center for Public Health Research, Nanjing University, Nanjing, PR China; State Key Lab of Analytical Chemistry for Life Science, Nanjing University, Nanjing, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Center for Public Health Research, Nanjing University, Nanjing, PR China.
authors.forename: Huanru
authors.initials: H
authors.lastname: Wang
authors.affiliation: Center for Public Health Research, Nanjing University, Nanjing, PR China.
authors.forename: Yixuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Nanjing Children's Hospital, Nanjing Medical University, Nanjing, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Center for Public Health Research, Nanjing University, Nanjing, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chu
authors.affiliation: Center for Public Health Research, Nanjing University, Nanjing, PR China.
authors.forename: Airong
authors.initials: A
authors.lastname: Su
authors.affiliation: Center for Public Health Research, Nanjing University, Nanjing, PR China; State Key Lab of Analytical Chemistry for Life Science, Nanjing University, Nanjing, PR China; Jiangsu Key Laboratory of Molecular Medicine, Medical School, Nanjing University, Nanjing, PR China. Electronic address: wzhw@nju.edu.cn.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wu
mesh_terms: D020121:5' Untranslated Regions; D029821:Enterovirus A, Human / Q000235:genetics* / Q000502:physiology*; D016679:Genome, Viral; D006801:Humans; D039102:Poly(A)-Binding Proteins / Q000502:physiology*; D012367:RNA, Viral / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000502:physiology*; D000074019:T-Cell Intracellular Antigen-1; D014779:Virus Replication
chemical_list: D020121:5' Untranslated Regions; D039102:Poly(A)-Binding Proteins; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D000074019:T-Cell Intracellular Antigen-1; C071558:TIA1 protein, human; C076751:TIAL1 protein, human
keywords: 5′-UTR; Enterovirus 71; RNA binding proteins; TIA-1; TIAR