title: Structural and functional analysis of endosomal compartments in epithelial cells.
abstract: Epithelial cells display segregated early endosomal compartments, termed apical sorting endosomes and basolateral sorting endosomes, that converge into a common late endosomal-lysosomal degradative compartment and common recycling endosomes (CREs). Unlike recycling endosomes of nonpolarized cells, CREs have the ability to sort apical and basolateral plasma membrane proteins into distinct apical and basolateral recycling routes, utilizing mechanisms similar to those employed by the trans Golgi network in the biosynthetic pathway. The apical recycling route includes an additional compartment, the apical recycling endosomes, consisting of multiple vesicles bundled around the basal body. Recent evidence indicates that, in addition to their role in internalizing ligands and recycling their receptors back to the cell surface, endosomal compartments act as intermediate stations in the biosynthetic routes to the plasma membrane. Here we review methods employed by our laboratory to study the endosomal compartments of epithelial cells and their multiple trafficking roles.
authors.affiliation: Margaret Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, NY, USA.
authors.forename: Andres E Perez
authors.initials: AE
authors.lastname: Bay
authors.affiliation: Margaret Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, NY, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Schreiner
authors.affiliation: Margaret Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, NY, USA.
authors.forename: Enrique
authors.initials: E
authors.lastname: Rodriguez-Boulan
mesh_terms: D000818:Animals; D004285:Dogs; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism* / Q000648:ultrastructure; D004847:Epithelial Cells / Q000378:metabolism* / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D061985:Madin Darby Canine Kidney Cells; D008856:Microscopy, Fluorescence; D021381:Protein Transport
keywords: Endocytosis; Endosomes; Epithelial cells; Recycling; Transcytosis; Transferrin receptor