title: The Single-Cell Lab or How to Perform Single-Cell Molecular Analysis.
abstract: The increasing interest towards cellular heterogeneity within cell populations has pushed the development of new protocols to isolate and analyze single cells. PCR-based amplification techniques are widely used in this field. However, setting up an experiment and analyzing the results can sometimes be challenging. The aim of this chapter is to provide a general overview on single-cell PCR analysis focusing on the potential pitfalls and on the possible solutions to successfully perform the analysis.
authors.affiliation: Leukocare AG, Martinsried, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Kirchner
authors.affiliation: Tumor Biology Laboratory, Department of Gynecology and Obstetrics, Ludwig-Maximilians-Univesity, Campus Innenstadt, Maistrasse 11, 80377, Munich, Germany. Marianna.Alunni@med.uni-muenchen.de.
authors.forename: Marianna
authors.initials: M
authors.lastname: Alunni-Fabbroni
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods / Q000592:standards; D016678:Genome; D023281:Genomics / Q000379:methods* / Q000592:standards*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000592:standards; D059010:Single-Cell Analysis / Q000379:methods* / Q000592:standards*
keywords: Cellular heterogeneity; Gene expression profiling; PCR; Single-cell analysis; Whole-genome amplification