title: Characterization of an Alkaline Family I.4 Lipase from Bacillus sp. W130-35 Isolated from a Tidal Mud Flat with Broad Substrate Specificity.
abstract: A gene encoding lipolytic enzyme, lip7-3, was isolated from Bacillus sp. W130-35 isolated from a tidal mud flat. The gene encoded a protein of 215 amino acids with a signal peptide composed of 34 amino acid residues. Lip7-3 belonged to the family I.4 lipase and showed its maximal activity at pH 9.0 and 60°C. Its activity increased in the presence of 30% methanol and, remarkably, increased as well to 154.6% in the presence of Ca(2+). Lip7-3 preferred pnitrophenyl octanoate (C8) as a substrate and exhibited broad specificity for short- to longchain fatty acid esters. Additionally, Lip7-3 showed a low degree of enantioselectivity for an S-enantiomer (e.g., (S)-methyl-3-hydroxy-2-methylpropionate). It efficiently hydrolyzed glyceryl tributyrate, but did not hydrolyze glyceryl trioleate, fish oil, or olive oil. Its substrate specificity and activation by the solvent might offer a merit to the biotechnological enzyme applications like transesterification in the production of biodiesel.
authors.affiliation: Department of Pharmacy, Sunchon National University, Suncheon 540-950, Republic of Korea.
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Research Institute of Life Pharmaceutical Sciences, Sunchon National University, Suncheon 540-950, Republic of Korea.
authors.forename: Won Kyeong
authors.initials: WK
authors.lastname: Jung
authors.affiliation: Department of Pharmacy, Sunchon National University, Suncheon 540-950, Republic of Korea.
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Sookmyung Women's University, Seoul 140-742, Republic of Korea.
authors.forename: Wanki
authors.initials: W
authors.lastname: Yoo
authors.affiliation: Department of Chemistry, Sookmyung Women's University, Seoul 140-742, Republic of Korea.
authors.forename: T Doohun
authors.initials: TD
authors.lastname: Kim
authors.affiliation: Department of Pharmacy, Sunchon National University, Suncheon 540-950, Republic of Korea.|Research Institute of Life Pharmaceutical Sciences, Sunchon National University, Suncheon 540-950, Republic of Korea.
authors.forename: Hoon
authors.initials: H
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D002118:Calcium / Q000378:metabolism; D004783:Environmental Microbiology; D020536:Enzyme Activators / Q000378:metabolism; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D020536:Enzyme Activators; D008049:Lipase; D002118:Calcium; D000432:Methanol
keywords: Bacillus sp. W130-35; Broad substrate specificity; Family I.4 lipase; Methanol activation; Tidal mud flat