title: Increased levels of MMP-3, MMP-9 and MPO represent predictors of in-stent restenosis, while increased levels of ADMA, LCAT, ApoE and ApoD predict bare metal stent patency.
abstract: OBJECTIVE
  We sought to identify biochemical predictors that indicate susceptibility to in-stent restenosis (ISR) after coronary artery bare-metal stenting.
  
  
  METHODS
  A total of 111 consecutive patients with post-percutaneous coronary intervention (PCI) in-stent restenosis of a target lesion within 12 months were matched for age, sex, vessel diameter, and diabetes with 111 controls without post-PCI ISR. Plasma or serum levels of biochemical markers were measured: matrix metalloproteinases (MMP) 2, 3, 9; myeloperoxidase (MPO); asymmetric dimethylarginine (ADMA); lipoprotein (a) (Lp[a]); apolipoproteins E and D (ApoE and D); and lecitin-cholesterol acyltransferase (LCAT). Multivariable logistic regression association tests were performed.
  
  
  RESULTS
  Increased plasma MMP-3 (OR: 1.013; 95% CI: 1.004-1.023; P = 0.005), MMP-9 (OR: 1.014; 95% CI: 1.008-1.020; P < 0.0001) or MPO (OR: 1,003; 95% CI: 1.001-1.005; P = 0.002) was significantly associated with increased risk of ISR. Increased levels of ADMA (OR: 0.212; 95% CI: 0.054-0.827; P = 0.026), ApoE (OR: 0.924; 95% CI: 0.899-0.951; P < 0.0001), ApoD (OR: 0.919; 95% CI: 0.880-0.959; P = 0.0001), or LCAT (OR: 0.927; 95% CI: 0.902-0.952; P < 0.0001) was associated with risk reduction. No correlation was found between plasma MMP-2 or Lp (a) and ISR risk.
  
  
  CONCLUSIONS
  Increased levels of MMP-3, MMP-9, and MPO represent predictors of ISR after bare-metal stent implantation. In contrast, increased ADMA, LCAT, and Apo E and D indicate a decreased in-stent restenosis occurrence.
authors.affiliation: Department of Clinical Studies, Faculty of Medicine, University of Ostrava, Czech Republic.|Department of Cardiovascular Diseases, University Hospital Ostrava.
authors.forename: Leos
authors.initials: L
authors.lastname: Pleva
authors.affiliation: Department of Biomedical Sciencies, Faculty of Medicine, University of Ostrava.|Department of Laboratory Medicine, University Hospital Ostrava.
authors.forename: Pavlina
authors.initials: P
authors.lastname: Kusnierova
authors.affiliation: Department of Biomedical Sciencies, Faculty of Medicine, University of Ostrava.|Department of Medical Genetics, University Hospital Ostrava.
authors.forename: Pavlina
authors.initials: P
authors.lastname: Plevova
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University Olomouc.
authors.forename: Jana
authors.initials: J
authors.lastname: Zapletalova
authors.affiliation: R&D Department, Biovendor Inc., Brno.
authors.forename: Michal
authors.initials: M
authors.lastname: Karpisek
authors.affiliation: Department of Biomedical Sciencies, Faculty of Medicine, University of Ostrava.|Department of Medical Genetics, University Hospital Ostrava.
authors.forename: Lucie
authors.initials: L
authors.lastname: Faldynova
authors.affiliation: Department of Biomedical Sciencies, Faculty of Medicine, University of Ostrava.|Blood Center, University Hospital of Ostrava.
authors.forename: Petra
authors.initials: P
authors.lastname: Kovarova
authors.affiliation: Department of Cardiovascular Diseases, University Hospital Ostrava.
authors.forename: Pavel
authors.initials: P
authors.lastname: Kukla
mesh_terms: D000368:Aged; D053399:Apolipoproteins D / Q000378:metabolism; D001057:Apolipoproteins E / Q000378:metabolism; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D016022:Case-Control Studies; D023903:Coronary Restenosis / Q000175:diagnosis* / Q000503:physiopathology; D005260:Female; D006083:Graft Occlusion, Vascular / Q000175:diagnosis* / Q000503:physiopathology; D006801:Humans; D017270:Lipoprotein(a) / Q000378:metabolism; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008875:Middle Aged; D062645:Percutaneous Coronary Intervention; D009195:Peroxidase / Q000378:metabolism; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000378:metabolism; D015607:Stents; D014654:Vascular Patency / Q000502:physiology
chemical_list: D053399:Apolipoproteins D; D001057:Apolipoproteins E; D015415:Biomarkers; D017270:Lipoprotein(a); C018524:N,N-dimethylarginine; D001120:Arginine; D009195:Peroxidase; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; D019278:Matrix Metalloproteinase 3; D020780:Matrix Metalloproteinase 9
keywords: apolipoproteins D; apolipoproteins E; asymmetric dimethylarginine; in-stent restenosis; lecitin-cholesterol acyltransferase; matrix metalloproteinases; myeloperoxidase