title: Immune gene expression profiling of PBMC isolated from horses vaccinated with attenuated African horsesickness virus serotype 4.
abstract: Development of African horsesickness (AHS) subunit vaccines will have to include a rational approach that uses knowledge of how the virus interacts with the host immune system. The global in vivo immune response induced by attenuated AHSV serotype 4 in horses was characterised using transcriptome sequencing. PBMC were collected with 24h intervals for four days after inoculation and four days after a second boost, 21 days later. Transcriptome data were normalised to the day 0 naïve transcriptome and up- or down-regulated immune genes identified using the CLC workbench. Peak expression was observed 24h after each inoculation. Innate immunity was up-regulated after both inoculations and was characterised by type-1 interferon activation via the RIG-1/MDA5 pathway and the up-regulation of complement cascade components. After the second boost an adaptive immune response could be identified that included the production of cytokines indicative of T helper (Th)1, Th2 and Th17 responses.
authors.affiliation: ARC-Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort 0110, South Africa. Electronic address: PretoriusAL@arc.agric.za.
authors.forename: A
authors.initials: A
authors.lastname: Pretorius
authors.affiliation: ARC-Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort 0110, South Africa.
authors.forename: F E
authors.initials: FE
authors.lastname: Faber
authors.affiliation: ARC-Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort 0110, South Africa.
authors.forename: M
authors.initials: M
authors.lastname: van Kleef
mesh_terms: D000355:African Horse Sickness / Q000235:genetics / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D000356:African Horse Sickness Virus / Q000187:drug effects / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D003165:Complement System Proteins / Q000235:genetics / Q000276:immunology; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006736:Horses; D007110:Immunity, Active; D007113:Immunity, Innate / Q000187:drug effects; D007370:Interferon Type I / Q000235:genetics / Q000276:immunology*; D046228:Microarray Analysis; D065288:Serogroup; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000276:immunology* / Q000821:virology; D059467:Transcriptome / Q000276:immunology; D014611:Vaccination; D014613:Vaccines, Attenuated; D014765:Viral Vaccines / Q000008:administration & dosage*
chemical_list: D000914:Antibodies, Viral; D007370:Interferon Type I; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D003165:Complement System Proteins; D053487:DEAD-box RNA Helicases
keywords: AHSV4; Gene expression; PBMC; Transcriptome sequencing