title: In Vivo Long-Term Monitoring of Circulating Tumor Cells Fluctuation during Medical Interventions.
abstract: The goal of this research was to study the long-term impact of medical interventions on circulating tumor cell (CTC) dynamics. We have explored whether tumor compression, punch biopsy or tumor resection cause dissemination of CTCs into peripheral blood circulation using in vivo fluorescent flow cytometry and breast cancer-bearing mouse model inoculated with MDA-MB-231-Luc2-GFP cells in the mammary gland. Two weeks after tumor inoculation, three groups of mice were the subject of the following interventions: (1) tumor compression for 15 minutes using 400 g weight to approximate the pressure during mammography; (2) punch biopsy; or (3) surgery. The CTC dynamics were determined before, during and six weeks after these interventions. An additional group of tumor-bearing mice was used as control and did not receive an intervention. The CTC dynamics in all mice were monitored weekly for eight weeks after tumor inoculation. We determined that tumor compression did not significantly affect CTC dynamics, either during the procedure itself (P = 0.28), or during the 6-week follow-up. In the punch biopsy group, we observed a significant increase in CTC immediately after the biopsy (P = 0.02), and the rate stayed elevated up to six weeks after the procedure in comparison to the tumor control group. The CTCs in the group of mice that received a tumor resection disappeared immediately after the surgery (P = 0.03). However, CTC recurrence in small numbers was detected during six weeks after the surgery. In the future, to prevent these side effects of medical interventions, the defined dynamics of intervention-induced CTCs may be used as a basis for initiation of aggressive anti-CTC therapy at time-points of increasing CTC number.
authors.affiliation: Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas, United States of America; Department of General and Visceral Surgery, University hospital of Frankfurt, Frankfurt am Main, Germany.
authors.forename: Mazen A
authors.initials: MA
authors.lastname: Juratli
authors.affiliation: Department of Biostatistics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, United States of America.
authors.forename: Eric R
authors.initials: ER
authors.lastname: Siegel
authors.affiliation: Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas, United States of America.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Nedosekin
authors.affiliation: Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas, United States of America.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Sarimollaoglu
authors.affiliation: Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas, United States of America.
authors.forename: Azemat
authors.initials: A
authors.lastname: Jamshidi-Parsian
authors.affiliation: Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas, United States of America.
authors.forename: Chengzhong
authors.initials: C
authors.lastname: Cai
authors.affiliation: Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas, United States of America.
authors.forename: Yulian A
authors.initials: YA
authors.lastname: Menyaev
authors.affiliation: Department of Otolaryngology - Head and Neck Surgery, University of Arkansas for Medical Sciences, Little Rock, Arkansas, United States of America.
authors.forename: James Y
authors.initials: JY
authors.lastname: Suen
authors.affiliation: Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas, United States of America.
authors.forename: Ekaterina I
authors.initials: EI
authors.lastname: Galanzha
authors.affiliation: Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas, United States of America; Department of Otolaryngology - Head and Neck Surgery, University of Arkansas for Medical Sciences, Little Rock, Arkansas, United States of America.
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Zharov
mesh_terms: D000818:Animals; D001706:Biopsy / Q000009:adverse effects / Q000379:methods; D001943:Breast Neoplasms / Q000473:pathology* / Q000601:surgery / Q000628:therapy; D005434:Flow Cytometry; D064593:Heterografts; D008327:Mammography / Q000009:adverse effects; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis / Q000473:pathology; D009360:Neoplastic Cells, Circulating