title: Evolutionary dynamics of two satellite DNA families in rock lizards of the genus Iberolacerta (Squamata, Lacertidae): different histories but common traits.
abstract: Satellite DNAs compose a large portion of all higher eukaryotic genomes. The turnover of these highly repetitive sequences is an important element in genome organization and evolution. However, information about the structure and dynamics of reptilian satellite DNA is still scarce. Two satellite DNA families, HindIII and TaqI, have been previously characterized in four species of the genus Iberolacerta. These families showed different chromosomal locations, abundances, and evolutionary rates. Here, we extend the study of both satellite DNAs (satDNAs) to the remaining Iberolacerta species, with the aim to investigate the patterns of variability and factors influencing the evolution of these repetitive sequences. Our results revealed disparate patterns but also common traits in the evolutionary histories of these satellite families: (i) each satellite DNA is made up of a library of monomer variants or subfamilies shared by related species; (ii) species-specific profiles of satellite repeats are shaped by expansions and/or contractions of different variants from the library; (iii) different turnover rates, even among closely related species, result in great differences in overall sequence homogeneity and in concerted or non-concerted evolution patterns, which may not reflect the phylogenetic relationships among taxa. Contrasting turnover rates are possibly related to genomic constraints such as karyotype architecture and the interspersed organization of diverging repeat variants in satellite arrays. Moreover, rapid changes in copy number, especially in the centromeric HindIII satDNA, may have been associated with chromosomal rearrangements and even contributed to speciation within Iberolacerta.
authors.affiliation: Grupo de Investigación en Bioloxía Evolutiva, Departamento de Bioloxía Celular e Molecular, Universidade da Coruña, E-15071, A Coruña, Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: Rojo
authors.affiliation: Grupo de Investigación en Bioloxía Evolutiva, Departamento de Bioloxía Celular e Molecular, Universidade da Coruña, E-15071, A Coruña, Spain.
authors.forename: Andrés
authors.initials: A
authors.lastname: Martínez-Lage
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Giovannotti
authors.affiliation: Grupo de Investigación en Bioloxía Evolutiva, Departamento de Bioloxía Celular e Molecular, Universidade da Coruña, E-15071, A Coruña, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: González-Tizón
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Nisi Cerioni
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.|Consiglio Nazionale delle Ricerche, Istituto di Scienze Marine Sezione Pesca Marittima, Largo Fiera della Pesca, 60125, Ancona, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Caputo Barucchi
authors.affiliation: Grupo de Investigación en Bioloxía Evolutiva, Departamento de Bioloxía Animal, Bioloxía Vexetal e Ecoloxía, Universidade da Coruña, E-15071, A Coruña, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Galán
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.
authors.forename: Ettore
authors.initials: E
authors.lastname: Olmo
authors.affiliation: Grupo de Investigación en Bioloxía Evolutiva, Departamento de Bioloxía Celular e Molecular, Universidade da Coruña, E-15071, A Coruña, Spain. horacio.naveira.fachal@udc.es.
authors.forename: Horacio
authors.initials: H
authors.lastname: Naveira
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes; D016000:Cluster Analysis; D016384:Consensus Sequence; D004276:DNA, Satellite; D019143:Evolution, Molecular; D005260:Female; D050259:Genes, Mitochondrial; D056726:Genetic Association Studies; D014644:Genetic Variation; D017404:In Situ Hybridization, Fluorescence; D008116:Lizards / Q000145:classification / Q000235:genetics*; D008297:Male; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D019655:Quantitative Trait, Heritable; D017422:Sequence Analysis, DNA
chemical_list: D004276:DNA, Satellite
keywords: Concerted evolution; FISH; Iberolacerta; Library model; Satellite DNA; Squamate reptiles