title: Tissue-Specific Metabolic Profile Study ofMoringa oleifera L.Using Nuclear Magnetic Resonance Spectroscopy.
abstract: Moringa oleifera, an important multipurpose crop, is rich in various phytochemicals: flavonoids, antioxidants, vitamins, minerals and carotenes. The purpose of this study was to profile the groups of metabolites in leaf and stem tissues ofM. oleifera. Various sugars, amino acids, and organic acid derivatives were found in all of theM. oleiferatissues with different profiles/peak intensities depending on the tissue. 1D proton nuclear magnetic resonance (NMR) was applied for collecting metabolite spectra. Approximately 30 metabolites with 2 unknown peaks were identified with Chenomx and verified with MMCD databases using carbon data. Among these metabolites, 22 metabolites were identified as common in both leaf and stem tissues. Of the remaining 8 metabolites, 4-aminobutyrate, adenosine, guanosine, tyrosine, and p-cresol were found only in leaf tissues; however, glutamate, glutamine, and tryptophan were found only in stem tissues. Biochemical pathway analysis revealed that 28 identified metabolites were interconnected with 36 different pathways as well as related to different fatty acids and secondary metabolites synthesis biochemical pathways. It is well known that different tissues ofM. oleiferahave nutritional, medicinal, and therapeutic values; therefore, our main objective is to provide a publicly availableM. olieferatissue specific metabolite database.
authors.affiliation: Department of Biology, Claflin University, Orangeburg SC 29115.
authors.forename: Iqbal
authors.initials: I
authors.lastname: Mahmud
authors.affiliation: Department of Biology, Claflin University, Orangeburg SC 29115.
authors.forename: Kamal
authors.initials: K
authors.lastname: Chowdhury
authors.affiliation: Department of Chemistry, Claflin University, Orangeburg SC 29115.
authors.forename: Arezue
authors.initials: A
authors.lastname: Boroujerdi
keywords: Biochemical pathway analysis; Metabolomics profile; Moringa oleifera; Nuclear Magnetic Resonance; Spectrum