title: Effect of temperature on the release of carvacrol and cinnamaldehyde incorporated into polymeric systems to control growth and biofilms of Escherichia coli and Staphylococcus aureus.
abstract: This study assessed the effect of temperature on the release of essential oil components incorporated by melt compounding into polymeric films. Specifically, polyethylene-co-vinylacetate (EVA) films containing carvacrol (CAR) and cinnamaldehyde (ALD), alone and in combination, were prepared and their surface and mechanical properties and antibacterial and anti-biofilm activity against Escherichia coli and Staphylococcus aureus were evaluated. The addition of ALD and CAR did not provoke variation in the surface morphology of EVA and allowed their delivery. At 37°C, films containing CAR, ALD or their combination (25+75%) were found to have the strongest bactericidal effect, whereas at lower temperatures a lower killing rate was observed. There was no clear evidence of the influence of temperature on the anti-biofilm activity of the essential oil component-based polymeric films. The biomass formed on EVA containing ALD, CAR or their combination (25+75) was significantly lower (60-80% reduction) than that formed on the EVA control at both 37° and 22°C.
authors.affiliation: a Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute , University of Messina , Messina , Sicily , Italy.
authors.forename: A
authors.initials: A
authors.lastname: Nostro
authors.affiliation: b Dipartimento di Ingegneria Civile, Ambientale, Aerospaziale e dei Materiali , University of Palermo, UdR INSTM di Palermo , Palermo , Sicily , Italy.
authors.forename: R
authors.initials: R
authors.lastname: Scaffaro
authors.affiliation: b Dipartimento di Ingegneria Civile, Ambientale, Aerospaziale e dei Materiali , University of Palermo, UdR INSTM di Palermo , Palermo , Sicily , Italy.
authors.forename: L
authors.initials: L
authors.lastname: Botta
authors.affiliation: a Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute , University of Messina , Messina , Sicily , Italy.
authors.forename: A
authors.initials: A
authors.lastname: Filocamo
authors.affiliation: a Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute , University of Messina , Messina , Sicily , Italy.
authors.forename: A
authors.initials: A
authors.lastname: Marino
authors.affiliation: a Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute , University of Messina , Messina , Sicily , Italy.
authors.forename: G
authors.initials: G
authors.lastname: Bisignano
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D000081007:Cymenes; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D039821:Monoterpenes / Q000737:chemistry / Q000494:pharmacology; D009822:Oils, Volatile / Q000737:chemistry*; D011095:Polyethylenes / Q000737:chemistry / Q000494:pharmacology; D011145:Polyvinyls / Q000737:chemistry / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D000081007:Cymenes; D039821:Monoterpenes; D009822:Oils, Volatile; D011095:Polyethylenes; D011145:Polyvinyls; C477722:polyethylene-polyvinylacetate copolymer; D000171:Acrolein; C073316:carvacrol; C012843:cinnamaldehyde
keywords: Escherichia coli; Staphylococcus aureus; antibacterial polymers; biofilm; essential oil components; temperature