title: [Grape seed proanthocyanidin extracts inhibit lipopolysaccharide of Porphyromonas gingivalis].
abstract: OBJECTIVE
  To study the inhibitory effect of grape seed proanthocyanidin extracts on 1ipopolysacharides of Porphyromonas gingivalis.
  
  
  METHODS
  Grape seed proanthocyanidin extracts were double-diluted to different concentrations by two-fold dilution. The effect on Porphyromonas gingivalis was measured by minimal inhibitory concentration (MIC). The inhibition effect of grape seed proanthocyanidin extracts on lipopolysaccharide was measured by limulus assay with 6 concentrations below the minimal inhibitory concentration. SPSS 17.0 software package was used for statistical analysis.
  
  
  RESULTS
  The value of MIC of grape seed proanthocyanidin extracts was 0.8 mg/mL to the strains of the experimental bacteria. The inhibitory effect on lipopolysaccharide enhanced with the increasing of the concentration of grape seed proanthocyanidin extracts within the scope of 0.05 to 0.4 mg/mL (P<0.05,0.01).
  
  
  CONCLUSIONS
  Grape seed proanthocyanidin extracts have inhibitory effect on lipopolysaccharide of Porphyromonas gingivalis.
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital of Nanchang University. Nanchang 330006, Jiangxi Province, China.E-mail:cixiangke@163.com.
authors.forename: Xiang-ke
authors.initials: XK
authors.lastname: Ci
authors.forename: Li-pei
authors.initials: LP
authors.lastname: Chen
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Ou
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D056604:Grape Seed Extract / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D016966:Porphyromonas gingivalis / Q000187:drug effects*; D044945:Proanthocyanidins / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D008070:Lipopolysaccharides; D044945:Proanthocyanidins; C013221:proanthocyanidin