title: Characterization of estrogenic receptor agonists and evaluation of estrogenic activity in the sediments of Liaohe River protected areas.
abstract: Estrogenic activity of 12 sediment samples from Liaohe River protected areas was evaluated by the recombinant yeast bioassays. The bioassay-derived 17β-estradiol equivalents of crude extracts (Bio-EEQcrudes) were between 52.2 and 207.6pg/g dry weight. The most concerned estrogenic receptor (ER) agonists including estrone (E1), 17β-estradiol (E2), estriol (E3), 17α-ethynylestradiol (EE2), 4-nonylphenols (4-NP), bisphenol A (BPA), and organochlorine pesticides (OCPs) were determined. The concentrations of E1, E2, E3, EE2, BPA, andΣ10OCPs ranged up to 203.3pg/g, 185.8pg/g, 237.7pg/g, 188.5pg/g, 51.0ng/g, and 3.6ng/g, respectively. Taken together with polarity-based fractionation, in vitro bioassay and chemical analysis, it indicated that E1, E2, and EE2 were the predominant ER agonists and were mainly from the discharge of domestic wastewater and breeding wastewater. Meanwhile, this study showed that the establishment of protected areas had not obviously reduced the ecological risk caused by ER agonists in Liaohe River protected areas sediments.
authors.affiliation: College of Energy and Environment, Shenyang Aerospace University, Shenyang 110136, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ke
authors.affiliation: College of Energy and Environment, Shenyang Aerospace University, Shenyang 110136, China.
authors.forename: Chunyong
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Energy and Environment, Shenyang Aerospace University, Shenyang 110136, China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Land and Environment, Shenyang Agriculture University, Shenyang 110161, China. Electronic address: xingyun92757@163.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Energy and Environment, Shenyang Aerospace University, Shenyang 110136, China.
authors.forename: Shaofeng
authors.initials: S
authors.lastname: Gui
mesh_terms: D001559:Benzhydryl Compounds / Q000032:analysis; D001681:Biological Assay / Q000379:methods; D002681:China; D054750:Ecotoxicology / Q000379:methods*; D052244:Endocrine Disruptors / Q000032:analysis / Q000494:pharmacology; D004958:Estradiol / Q000032:analysis; D004964:Estriol / Q000032:analysis; D004967:Estrogens / Q000032:analysis* / Q000494:pharmacology*; D004970:Estrone / Q000032:analysis; D004997:Ethinyl Estradiol / Q000032:analysis; D019015:Geologic Sediments / Q000737:chemistry; D006843:Hydrocarbons, Chlorinated / Q000032:analysis; D010575:Pesticides / Q000032:analysis; D010636:Phenols / Q000032:analysis; D045483:Rivers / Q000737:chemistry; D062065:Wastewater; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000494:pharmacology; D015003:Yeasts / Q000235:genetics; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004958:Estradiol; D004964:Estriol; D004967:Estrogens; D004970:Estrone; D004997:Ethinyl Estradiol; D006843:Hydrocarbons, Chlorinated; D010575:Pesticides; D010636:Phenols; D062065:Wastewater; D014874:Water Pollutants, Chemical; C041594:4-nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Chemical analysis; Estrogen receptor (ER) agonists; In vitro bioassays; Polarity-based fractionation; Sediments