title: Repairing calvarial defects with biodegradable polycaprolactone-chitosan scaffolds fabricated using the melt stretching and multilayer deposition technique.
abstract: The ability to repair bone defects of polycaprolactone-chitosan scaffolds containing 20% chitosan (PCL-20%CS) fabricated using the melt stretching and multilayer deposition (MSMD) technique was assessed and compared with commercial scaffolds. Two calvarium defects of 11 mm in diameter were created in each of the fifteen New Zealand white rabbits. The PCL-20%CS scaffolds were implanted in one site (group A) while another site was performed with PCL-tricalcium phosphate (TCP) scaffolds containing 20% TCP (PCL-20%TCP) fabricated by fused deposition modeling technique (FDM) (group B). At two, four and eight weeks thereafter, new bone regeneration within the defects was assessed using histomorphometric and micro-computed tomography (µ-CT) analysis. The result of histological sections demonstrated that chronic inflammatory reaction was generally detected along scaffolds of group A, but it was not found in group B. Over 8 weeks, the µ-CT analysis indicated that the average amount of new bone of group A was slightly less than that of group B (p>0.05). In conclusion, efficacy of the PCL-20%CS MSMD scaffolds for repairing bone defects was less than that of the PCL-20%TCP FDM scaffolds. However, MSMD scaffolding is still the technique of choice, but needed some modifications.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hatyai, Thailand.
authors.forename: Nuttawut
authors.initials: N
authors.lastname: Thuaksuban
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hatyai, Thailand.
authors.forename: Thongchai
authors.initials: T
authors.lastname: Nuntanaranont
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hatyai, Thailand.
authors.forename: Srisurang
authors.initials: S
authors.lastname: Suttapreyasri
authors.affiliation: Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hatyai, Thailand.
authors.forename: Pleumjit
authors.initials: P
authors.lastname: Boonyaphiphat
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology; D002130:Calcium Phosphates / Q000737:chemistry*; D055119:Elastic Modulus; D019544:Equipment Failure Analysis; D048091:Guided Tissue Regeneration / Q000295:instrumentation / Q000379:methods; D006361:Heating; D008297:Male; D011091:Polyesters / Q000737:chemistry*; D011474:Prosthesis Design; D011817:Rabbits; D011859:Radiography; D012887:Skull Fractures / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*; D054457:Tissue Scaffolds; D016896:Treatment Outcome
chemical_list: D002130:Calcium Phosphates; D011091:Polyesters; C016240:polycaprolactone; C018392:tricalcium phosphate
keywords: Polycaprolactone; calvarium; chitosan; scaffold