title: Micromonospora nickelidurans sp. nov., isolated from soil from a nickel-mining site.
abstract: An actinomycete, strain K55T, was isolated from a composite soil sample from a nickel mine,collected from Yueyang, Shaanxi Province, PR China. Strain K55T showed 16S rRNA gene sequence similarities of 98.73 %–98.51 % to species of the genus Micromonospora, including Micromonospora haikouensis 232617T, Micromonospora coxensis 2-30-b(28)T, Micromonospora wenchangensis 2602GPT1-05T, Micromonospora matsumotoense IMSNU22003T, Micromonospora maoerensis NEAU-MES19T, and Micromonospora humi P0402T. This strain harboured meso-diaminopimelic acid, alanine and glycine as the major cell-wall amino acids, xylose and glucose as the characteristic whole-cell sugars, and iso-C15 : 0(20.53 %),iso-C17 : 0 (12.74 %), iso-C16 : 0 (12.15 %), anteiso-C17 : 0 (7.97 %), C17 : 1ω8c(7.49 %) and C17 : 0 (6.63 %) as the dominant fatty acids. The major menaquinones were MK-10(H4) and MK-10(H6). The phospholipid profile comprised phosphatidylethanolamine,diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol and unknown phosphoglycolipids. The DNA G+C content was 71.4 mol%. A comprehensive analysis ofseveral physiological and biochemical traits and DNA–DNA relatedness indicated that strainK55T was different from closely related species. These phenotypic, genotypic and chemotaxonomic data suggest that strain K55T represents a novel species of the genus Micromonospora, for which the name Micromonospora nickelidurans sp. nov., is proposed. The type strain is K55T (5JCM 30559T5ACCC19713T).
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling Shaanxi 712100, PR China.|Technology and Engineering Center of Biopesticide, Shaanxi Province/Research and Development Center of Biorational Pesticide, Northwest A & F University, Yangling 712100, China.
authors.forename: Yan Bing
authors.initials: YB
authors.lastname: Lin
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling Shaanxi 712100, PR China.
authors.forename: Miao Chun
authors.initials: MC
authors.lastname: Fan
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling Shaanxi 712100, PR China.
authors.forename: Yan Qing
authors.initials: YQ
authors.lastname: Guo
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling Shaanxi 712100, PR China.
authors.forename: Xiao Hui
authors.initials: XH
authors.lastname: Di
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling Shaanxi 712100, PR China.
authors.forename: Dan Hong
authors.initials: DH
authors.lastname: Dong
authors.affiliation: Technology and Engineering Center of Biopesticide, Shaanxi Province/Research and Development Center of Biorational Pesticide, Northwest A & F University, Yangling 712100, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling Shaanxi 712100, PR China.
authors.forename: Ge Hong
authors.initials: GH
authors.lastname: Wei
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008847:Micromonospora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008906:Mining; D008969:Molecular Sequence Data; D009532:Nickel; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; D009532:Nickel