title: An electrochemical nanobiosensor for plasma miRNA-155, based on graphene oxide and gold nanorod, for early detection of breast cancer.
abstract: Circulating miRNAs are emerging as novel reliable biomarkers for early detection of cancer diseases. Through combining the advantages of electrochemical methods and nanomaterials with the selectivity of the oligo-hybridization-based biosensors, a novel electrochemical nanobiosensor for plasma miR-155 detection have demonstrated here, based on thiolated probe-functionalized gold nanorods (GNRs) decorated on the graphene oxide (GO) sheet on the surface of the glassy carbon electrode (GCE). The reduction signals of a novel intercalating label Oracet Blue (OB), were measured by differential pulse voltammetry (DPV) method. The transmission electron microscope (TEM) imaging, UV-vis spectrophotometry, cyclic voltammetry (CV), field emission scanning electron microscope (FE-SEM) imaging and energy dispersive spectroscopy (EDS) were proved the right synthesis of the GNRs and correct assembly of the modified electrode. The electrochemical signal had a linear relationship with the concentration of the target miRNA ranging from 2.0 fM to 8.0 pM, and the detection limit was 0.6 fM. Furthermore, the nanobiosensor showed high Specificity, and was able to discriminate sharply between complementary target miRNA, single-, three-base mismatch, and non-complementary miRNA. Alongside the outstanding sensitivity and selectivity, this nanobiosensor had great storage ability, reproducibility, and showed a decent response in the real sample analysis with plasma. In conclusion, the proposed electrochemical nanobiosensor could clinically be used in the early detection of the breast cancer, by direct detection of the plasma miR-155 in real clinical samples, without a need for sample preparation, RNA extraction and/or amplification.
authors.affiliation: Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, PO Box: 14395-1561, Tehran, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Azimzadeh
authors.affiliation: Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, PO Box: 14395-1561, Tehran, Iran. Electronic address: mrahaie@ut.ac.ir.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Rahaie
authors.affiliation: Department of Textile and Polymer Engineering, Yazd Branch, Islamic Azad University, PO Box: 89195-155, Yazd, Iran. Electronic address: nasirizadeh@iauyazd.ac.ir.
authors.forename: Navid
authors.initials: N
authors.lastname: Nasirizadeh
authors.affiliation: Department of Nanobiotechnology/Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, PO Box: 14115-154, Tehran, Iran.
authors.forename: Khadijeh
authors.initials: K
authors.lastname: Ashtari
authors.affiliation: Department of Nanobiotechnology/Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, PO Box: 14115-154, Tehran, Iran. Electronic address: naderman@modares.ac.ir.
authors.forename: Hossein
authors.initials: H
authors.lastname: Naderi-Manesh
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation*; D001943:Breast Neoplasms / Q000097:blood / Q000175:diagnosis*; D003217:Conductometry / Q000295:instrumentation*; D055088:Early Detection of Cancer / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D035683:MicroRNAs / Q000097:blood*; D036103:Nanotechnology / Q000295:instrumentation; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D010087:Oxides; D006046:Gold; D006108:Graphite
keywords: Breast cancer; Circulating miRNA; Early detection; Electrochemical; MiR-155; Nanobiosensor