title: Characterisation of insulin-producing cells differentiated from tonsil derived mesenchymal stem cells.
abstract: Tonsil-derived (T-) mesenchymal stem cells (MSCs) display mutilineage differentiation potential and self-renewal capacity and have potential as a banking source. Diabetes mellitus is a prevalent disease in modern society, and the transplantation of pancreatic progenitor cells or various stem cell-derived insulin-secreting cells has been suggested as a novel therapy for diabetes. The potential of T-MSCs to trans-differentiate into pancreatic progenitor cells or insulin-secreting cells has not yet been investigated. We examined the potential of human T-MSCs to trans-differentiate into pancreatic islet cells using two different methods based on β-mercaptoethanol and insulin-transferin-selenium, respectively. First, we compared the efficacy of the two methods for inducing differentiation into insulin-producing cells. We demonstrated that the insulin-transferin-selenium method is more efficient for inducing differentiation into insulin-secreting cells regardless of the source of the MSCs. Second, we compared the differentiation potential of two different MSC types: T-MSCs and adipose-derived MSCs (A-MSCs). T-MSCs had a differentiation capacity similar to that of A-MSCs and were capable of secreting insulin in response to glucose concentration. Islet-like clusters differentiated from T-MSCs had lower synaptotagmin-3, -5, -7, and -8 levels, and consequently lower secreted insulin levels than cells differentiated from A-MSCs. These results imply that T-MSCs can differentiate into functional pancreatic islet-like cells and could provide a novel, alternative cell therapy for diabetes mellitus.
authors.affiliation: Department of Biochemistry, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, Seoul 158-710, Republic of Korea.
authors.forename: So-Yeon
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, Seoul 158-710, Republic of Korea.
authors.forename: Ye-Ryung
authors.initials: YR
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, School of Medicine, Gachon University, Yeonsu-gu, Incheon 406-799, Republic of Korea.
authors.forename: Woo-Jae
authors.initials: WJ
authors.lastname: Park
authors.affiliation: Departments of Otorhinolaryngology - Head and Neck Surgery, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, Seoul 158-710, Republic of Korea; Departments of Ewha Global Top 5 Research Program, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, Seoul 158-710, Republic of Korea.
authors.forename: Han Su
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, Seoul 158-710, Republic of Korea.
authors.forename: Sung-Chul
authors.initials: SC
authors.lastname: Jung
authors.affiliation: Departments of Microbiology, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, Seoul 158-710, Republic of Korea.
authors.forename: So-Youn
authors.initials: SY
authors.lastname: Woo
authors.affiliation: Departments of Molecular Medicine, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, Seoul 158-710, Republic of Korea; Departments of Ewha Global Top 5 Research Program, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, Seoul 158-710, Republic of Korea.
authors.forename: Inho
authors.initials: I
authors.lastname: Jo
authors.affiliation: Departments of Pediatrics, and School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, Seoul 158-710, Republic of Korea; Departments of Ewha Global Top 5 Research Program, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, Seoul 158-710, Republic of Korea. Electronic address: ykh@ewha.ac.kr.
authors.forename: Kyung-Ha
authors.initials: KH
authors.lastname: Ryu
authors.affiliation: Department of Biochemistry, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, Seoul 158-710, Republic of Korea. Electronic address: joowon.park@ewha.ac.kr.
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Park
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D054338:Cell Transdifferentiation; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D000067470:Cellular Reprogramming Techniques; D003921:Diabetes Mellitus, Experimental / Q000601:surgery; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000637:transplantation; D008623:Mercaptoethanol / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D014066:Palatine Tonsil / Q000166:cytology* / Q000187:drug effects; D012643:Selenium / Q000494:pharmacology; D050857:Synaptotagmins / Q000172:deficiency; D014168:Transferrin / Q000494:pharmacology
chemical_list: D007328:Insulin; D014168:Transferrin; D050857:Synaptotagmins; D008623:Mercaptoethanol; D012643:Selenium
keywords: Adipose tissue; Diabetes; Insulin; Mesenchymal stem cell; Tonsil