title: Rhamnolipid and surfactin production from olive oil mill waste as sole carbon source.
abstract: Olive mill waste (OMW) creates a major environmental problem due to the difficulty of further waste processing. In this work we present an approach to give OMW added value by using it for the production of biosurfactants. Two bacterial species, Pseudomonas aeruginosa and Bacillus subtilis, were grown with OMW as the sole carbon source. Glycerol and waste frying oil were used as comparative carbon sources. B. subtilis produced surfactin (a lipopeptide) at a maximum concentration of 3.12 mg/L with 2% w/v of OMW in the medium, dropping to 0.57 mg/L with 10% w/v of OMW. In contrast, P. aeruginosa produced 8.78 mg/L of rhamnolipid with 2% w/v OMW increasing to 191.46 mg/L with 10% w/v OMW. The use of solvent-extracted OMW reduced the biosurfactant production by 70.8% and 88.3% for B. subtilis and P. aeruginosa respectively. These results confirm that OMW is a potential substrate for biosurfactant production.
authors.affiliation: Chemical Engineering Department, Faculty of Sciences, University of Granada, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Moya Ramírez
authors.affiliation: School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, N. Ireland, UK.
authors.forename: Konstantina
authors.initials: K
authors.lastname: Tsaousi
authors.affiliation: School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, N. Ireland, UK.
authors.forename: Michelle
authors.initials: M
authors.lastname: Rudden
authors.affiliation: School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, N. Ireland, UK.
authors.forename: Roger
authors.initials: R
authors.lastname: Marchant
authors.affiliation: Chemical Engineering Department, Faculty of Sciences, University of Granada, Spain.
authors.forename: Encarnación
authors.initials: E
authors.lastname: Jurado Alameda
authors.affiliation: Chemical Engineering Department, Faculty of Sciences, University of Granada, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: García Román
authors.affiliation: School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, N. Ireland, UK. Electronic address: im.banat@ulster.ac.uk.
authors.forename: Ibrahim M
authors.initials: IM
authors.lastname: Banat
mesh_terms: D001412:Bacillus subtilis; D002244:Carbon / Q000378:metabolism*; D006017:Glycolipids / Q000096:biosynthesis*; D055666:Lipopeptides / Q000096:biosynthesis*; D000069463:Olive Oil / Q000378:metabolism*; D010456:Peptides, Cyclic / Q000096:biosynthesis*; D010938:Plant Oils; D011550:Pseudomonas aeruginosa
chemical_list: D006017:Glycolipids; D055666:Lipopeptides; D000069463:Olive Oil; D010456:Peptides, Cyclic; D010938:Plant Oils; C418382:rhamnolipid; C009365:surfactin peptide; D002244:Carbon
keywords: Alperujo; Biosurfactants; Olive mill waste; Rhamnolipids; Surfactin