title: GPo1 alkB gene expression for improvement of the degradation of diesel oil by a bacterial consortium.
abstract: To facilitate the biodegradation of diesel oil, an oil biodegradation bacterial consortium was constructed. The alkane hydroxylase (alkB) gene of Pseudomonas putida GPo1 was constructed in a pCom8 expression vector, and the pCom8-GPo1 alkB plasmid was transformed into Escherichia coli DH5α. The AlkB protein was expressed by diesel oil induction and detected through SDS-polyacrylamide gel electrophoresis. The culture of the recombinant (pCom8-GPo1 alkB/E. coli DH5α) with the oil biodegradation bacterial consortium increased the degradation ratio of diesel oil at 24 h from 31% to 50%, and the facilitation rates were increased as the proportion of pCom8-GPo1 alkB/E. coli DH5α to the consortium increased. The results suggested that the expression of the GPo1 gene in E. coli DH5α could enhance the function of diesel oil degradation by the bacterial consortium.
authors.affiliation: The Naval Medical Research Institute, Shanghai, China .
authors.forename: Qun
authors.initials: Q
authors.lastname: Luo
authors.affiliation: The Naval Medical Research Institute, Shanghai, China .
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.affiliation: The Naval Medical Research Institute, Shanghai, China .
authors.forename: Deng-Yong
authors.initials: DY
authors.lastname: Hou
authors.affiliation: The Naval Medical Research Institute, Shanghai, China .
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Zhang
authors.affiliation: The Naval Medical Research Institute, Shanghai, China .
authors.forename: Xian-Rong
authors.initials: XR
authors.lastname: Shen
mesh_terms: D000150:Acinetobacter / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D042926:Cytochrome P-450 CYP4A / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D005648:Fuel Oils; D005742:Gasoline; D005818:Genetic Engineering; D059013:Microbial Consortia / Q000235:genetics*; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D010957:Plasmids / Q000235:genetics; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D005648:Fuel Oils; D005742:Gasoline; D042926:Cytochrome P-450 CYP4A
keywords: Escherichia coli DH5α; alkane hydroxylase; biodegradation; diesel oil; expression