title: Development of a molecular recognition based approach for multi-residue extraction of estrogenic endocrine disruptors from biological fluids coupled to liquid chromatography-tandem mass spectrometry measurement.
abstract: Multi-residue methods permitting the high-throughput and affordable simultaneous determination of an extended range of endocrine disrupting chemicals (EDCs) with reduced time and cost of analysis is of prime interest in order to characterize a whole set of bioactive compounds. Such a method based on UHPLC-MS/MS measurement and dedicated to 13 estrogenic EDCs was developed and applied to biological matrices. Two molecular recognition-based strategies, either molecular imprinted polymer (MIP) with phenolic template or estrogen receptors (ERα) immobilized on a sorbent, were assessed in terms of recovery and purification efficiency. Both approaches demonstrated their suitability to measure ultra-trace levels of estrogenic EDCs in aqueous samples. Applicability of the MIP procedure to urine and serum samples has also been demonstrated.
authors.affiliation: LUNAM Université, Oniris, USC 1329 Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), 44307, Nantes, France.|AgroParisTech, UMR1145 Ingénierie Procédés Aliments, 75005, Paris, France.
authors.forename: Radia
authors.initials: R
authors.lastname: Bousoumah
authors.affiliation: LUNAM Université, Oniris, USC 1329 Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), 44307, Nantes, France. laberca@oniris-nantes.fr.|INRA, 44307, Nantes, France. laberca@oniris-nantes.fr.|INRA, 75005, Paris, France. laberca@oniris-nantes.fr.
authors.forename: Jean Philippe
authors.initials: JP
authors.lastname: Antignac
authors.affiliation: AgroParisTech, UMR1145 Ingénierie Procédés Aliments, 75005, Paris, France.|INRA, 44307, Nantes, France.|INRA, 75005, Paris, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Camel
authors.affiliation: INSERM Institut National de la santé et de la Recherche Médicale, U1194 IRCM, Institut régional du Cancer Montpellier, Université Montpellier, 34298, Montpellier, France.
authors.forename: Marina
authors.initials: M
authors.lastname: Grimaldi
authors.affiliation: INSERM Institut National de la santé et de la Recherche Médicale, U1194 IRCM, Institut régional du Cancer Montpellier, Université Montpellier, 34298, Montpellier, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Balaguer
authors.affiliation: Département Sciences de l'Environnement et Santé Publique, Faculté de Pharmacie, UMR Hydrosciences, Université Montpellier 1, 34298, Montpellier, France.
authors.forename: Frederique
authors.initials: F
authors.lastname: Courant
authors.affiliation: LUNAM Université, Oniris, USC 1329 Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), 44307, Nantes, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Bichon
authors.affiliation: LUNAM Université, Oniris, USC 1329 Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), 44307, Nantes, France.
authors.forename: Marie-Line
authors.initials: ML
authors.lastname: Morvan
authors.affiliation: LUNAM Université, Oniris, USC 1329 Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), 44307, Nantes, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Le Bizec
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D052244:Endocrine Disruptors / Q000097:blood* / Q000302:isolation & purification / Q000652:urine*; D047628:Estrogen Receptor alpha / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000097:blood* / Q000302:isolation & purification / Q000652:urine*
chemical_list: D052244:Endocrine Disruptors; D047628:Estrogen Receptor alpha; D055767:Immobilized Proteins; D014874:Water Pollutants, Chemical; D014867:Water
keywords: Biological matrices; Estrogen receptors; Estrogenic endocrine disrupting chemicals; Molecular imprinted polymer; UHPLC-MS/MS