title: Decreased Osteogenic Activity of Mesenchymal Stem Cells in Patients With Corticosteroid-Induced Osteonecrosis of the Femoral Head.
abstract: BACKGROUND
  Osteonecrosis (ON) of the femoral head occurs when cells of trabecular bone spontaneously die. Mesenchymal stem cells (MSCs) have been introduced into the femoral head in an attempt to halt progression of the disease. The purpose of this study was to functionally compare MSCs in patients with ON of the femoral head with patients without.
  
  
  METHODS
  Mesenchymal stem cells were isolated from 20 patients with corticosteroid-induced ON and 10 controls without. Colony-forming unit and proliferation assays were used to assess MSC proliferation. Mesenchymal stem cells were differentiated into bone, fat, and cartilage. Functional assays were used to quantify the differentiation capacity.
  
  
  RESULTS
  Control MSCs demonstrated greater cellular growth potential and improved ability to differentiate into bone.
  
  
  CONCLUSIONS
  The decreased ability to differentiate into bone may be a reason why patients treated with autologous MSC infusion fail regenerative treatment strategies and progress to collapse.
authors.affiliation: Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota; Center for Regenerative Medicine, Mayo Clinic, Rochester, Minnesota.
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Houdek
authors.affiliation: Mayo Medical School, Mayo Clinic, Rochester, Minnesota; Center for Regenerative Medicine, Mayo Clinic, Rochester, Minnesota.
authors.forename: Cody C
authors.initials: CC
authors.lastname: Wyles
authors.affiliation: Center for Regenerative Medicine, Mayo Clinic, Rochester, Minnesota.
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Packard
authors.affiliation: Division of Cardiovascular Diseases, Mayo Clinic, Rochester, Minnesota; Center for Regenerative Medicine, Mayo Clinic, Rochester, Minnesota.
authors.forename: Andre
authors.initials: A
authors.lastname: Terzic
authors.affiliation: Division of Cardiovascular Diseases, Mayo Clinic, Rochester, Minnesota; Center for Regenerative Medicine, Mayo Clinic, Rochester, Minnesota.
authors.forename: Atta
authors.initials: A
authors.lastname: Behfar
authors.affiliation: Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota; Center for Regenerative Medicine, Mayo Clinic, Rochester, Minnesota.
authors.forename: Rafael J
authors.initials: RJ
authors.lastname: Sierra
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018450:Disease Progression; D005260:Female; D005271:Femur Head Necrosis / Q000139:chemically induced / Q000503:physiopathology / Q000628:therapy*; D005938:Glucocorticoids / Q000009:adverse effects*; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008875:Middle Aged; D055815:Young Adult
chemical_list: D005938:Glucocorticoids
keywords: avascular necrosis; femoral head; osteonecrosis; regenerative medicine; stem cells