title: Aligned Electroactive TMV Nanofibers as Enabling Scaffold for Neural Tissue Engineering.
abstract: Electroactive nanofibers were fabricated by in situ polymerization of aniline on the surface of tobacco mosaic virus (TMV) using sodium poly(styrenesulfonate) (PSS) as dopant. These electroactive TMV/PANi/PSS nanofibers were employed to support growth of neuronal cells, resulting in augmentation of the length of neurites. In addition, the percentage of cells with neurites was increased in comparison to cells cultured on TMV-derived nonconductive nanofibers. The TMV-based electroactive nanofibers could be aligned in capillaries that could guide the outgrowth direction of neurites, increase the percentage of cells with neurites, and lead to a bipolar cellular morphology. Our results demonstrate that the electroactivity and topographical cues provided by TMV/PANi/PSS nanofibers can synergistically stimulate neural cells differentiation and neurites outgrowth, which make it a promising scaffolding material for neural tissue engineering.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, P. R. China.|University of Chinese Academy of Sciences , Beijing 100049, P. R. China.
authors.forename: Yehong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Chemistry and Biochemistry, University of South Carolina , 631 Sumter Street, Columbia, South Carolina 29208, United States.
authors.forename: Sheng
authors.initials: S
authors.lastname: Feng
authors.affiliation: Department of Chemistry and Biochemistry, University of South Carolina , 631 Sumter Street, Columbia, South Carolina 29208, United States.
authors.forename: Xingjie
authors.initials: X
authors.lastname: Zan
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, P. R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, P. R. China.|Department of Chemistry and Biochemistry, University of South Carolina , 631 Sumter Street, Columbia, South Carolina 29208, United States.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry*; D009417:Nerve Tissue / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D016501:Neurites / Q000187:drug effects* / Q000378:metabolism; D016716:PC12 Cells; D011137:Polystyrenes / Q000737:chemistry; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014027:Tobacco Mosaic Virus
chemical_list: D000814:Aniline Compounds; D020099:Coated Materials, Biocompatible; D011137:Polystyrenes; C003321:polystyrene sulfonic acid; C023650:aniline