title: Optical mesoscopy without the scatter: broadband multispectral optoacoustic mesoscopy.
abstract: Optical mesoscopy extends the capabilities of biological visualization beyond the limited penetration depth achieved by microscopy. However, imaging of opaque organisms or tissues larger than a few hundred micrometers requires invasive tissue sectioning or chemical treatment of the specimen for clearing photon scattering, an invasive process that is regardless limited with depth. We developed previously unreported broadband optoacoustic mesoscopy as a tomographic modality to enable imaging of optical contrast through several millimeters of tissue, without the need for chemical treatment of tissues. We show that the unique combination of three-dimensional projections over a broad 500 kHz-40 MHz frequency range combined with multi-wavelength illumination is necessary to render broadband multispectral optoacoustic mesoscopy (2B-MSOM) superior to previous optical or optoacoustic mesoscopy implementations.
authors.affiliation: Chair for Biologial Imaging, Technische Universität München (TUM), Ismaningerstr. 22, 81675, Munich, Germany ; Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany.
authors.forename: Andrei
authors.initials: A
authors.lastname: Chekkoury
authors.affiliation: Chair for Biologial Imaging, Technische Universität München (TUM), Ismaningerstr. 22, 81675, Munich, Germany ; Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Gateau
authors.affiliation: Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany ; iThera Medical, GmbH, Zielstattstraße 13, 81379, Munich, Germany.
authors.forename: Wouter
authors.initials: W
authors.lastname: Driessen
authors.affiliation: Chair for Biologial Imaging, Technische Universität München (TUM), Ismaningerstr. 22, 81675, Munich, Germany ; Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Symvoulidis
authors.affiliation: Chair for Biologial Imaging, Technische Universität München (TUM), Ismaningerstr. 22, 81675, Munich, Germany ; Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bézière
authors.affiliation: Research Unit of Analytical Pathology, Institute of Pathology, Helmholtz ZentrumMünchen, IngolstädterLandstraße 1, 85764 Neuherberg, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Feuchtinger
authors.affiliation: Research Unit of Analytical Pathology, Institute of Pathology, Helmholtz ZentrumMünchen, IngolstädterLandstraße 1, 85764 Neuherberg, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Walch
authors.affiliation: Chair for Biologial Imaging, Technische Universität München (TUM), Ismaningerstr. 22, 81675, Munich, Germany ; Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany.
authors.forename: Vasilis
authors.initials: V
authors.lastname: Ntziachristos
keywords: (110.4234) Multispectral and hyperspectral imaging; (110.5120) Photoacoustic imaging; (110.5125) Photoacoustics; (110.6955) Tomographic imaging; (330.6180) Spectral discrimination