title: Quantification of complanatoside A in rat plasma using LC-MS/MS and its application to a pharmacokinetic study.
abstract: Complanatoside A is a flavonol glycoside isolated from Astragalus complanatus, and currently it is used as a quality control index for A. complanatus in the 2010 edition of the Chinese Pharmacopoeia. For the first time, a simple and sensitive LC-MS/MS method was developed for the determination of complanatoside A in rat plasma over the range of 2.3-575 ng/mL. Complanatoside A was extracted from plasma by a protein precipitation procedure, separated by LC and detected by MS/MS in positive electrospray ionization mode. The method was validated for selectivity, carryover, sensitivity, linearity, extraction recovery, matrix effect, accuracy, precision and stability studies. The lower limit of quantification was established at 2.3 ng/mL. Intra- and inter-day precisions (LLOQ, low-QC, med-QC and high-QC) were <7.9%, and accuracies were between 94.0 and 105.1%. Matrix effect was acceptable (97.9-103.0%) and extraction recovery was reproducible (88.5-94.4%). Complanatoside A was stable in the investigated conditions. The method was applied to the pharmacokinetics of complanatoside A in rats. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Department of Neonatology, the First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Echocardiography, the First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Clinical Medicine, Norman Bethune Medical College of Jilin University, Changchun, 130021, China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Geriatrics, the First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Zhouxia
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Department of Emergency, the First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Li
authors.initials: L
authors.lastname: Pang
authors.affiliation: Department of Geriatrics, the First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Jianmeng
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D044948:Flavonols / Q000097:blood* / Q000493:pharmacokinetics; D005960:Glucosides / Q000097:blood* / Q000493:pharmacokinetics; D057230:Limit of Detection; D051381:Rats; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D044948:Flavonols; D005960:Glucosides; C057070:complanatuside
keywords: LC-MS/MS; complanatoside A; pharmacokinetics; rat plasma