title: Microbial abatement of toluene using Aspergillus niger in upflow bioreactor.
abstract: Microbial abatement of toluene using Aspergillus niger in coir packed upflow bioreactor was investigated in this study. Toluene degrading microbes were isolated from municipal sewage effluent and identified by 16s rRNA sequencing method. The microbes were cultured in 2% (v/v) toluene input per day, which exhibited 95% removal efficiency with the kinetic correction value (R2) of 0.9024 at the optimum flow rate of about 0.4m3h-1. Various parameters such as effect of flow rate, column height, elimination capacity and EBRT with removal efficiency for 50 day cycle were also optimized. The plug flow model for toluene degradation was properly expressed and the Monod kinetics constant Kmand rmaxvalues were determined as 2.25gm-3and 67.773gm-3h-1respectively for microbial growth rate.
authors.affiliation: Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Chennai 603308, India. Electronic address: mpgopinath1980@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Gopinath
authors.affiliation: Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Chennai 603308, India.
authors.forename: C
authors.initials: C
authors.lastname: Mohanapriya
authors.affiliation: Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Chennai 603308, India.
authors.forename: K
authors.initials: K
authors.lastname: Sivakumar
authors.affiliation: Department of Biotechnology, St Joseph's College of Engineering, Chennai 600119, India.
authors.forename: G
authors.initials: G
authors.lastname: Baskar
authors.affiliation: Department of Industrial Biotechnology, Government College of Technology, Coimbatore 641013, India.
authors.forename: C
authors.initials: C
authors.lastname: Muthukumaran
authors.affiliation: Department of Chemical Engineering, Annamalai University, Chidambaram 608002, India.
authors.forename: R
authors.initials: R
authors.lastname: Dhanasekar
mesh_terms: D000393:Air Pollutants / Q000378:metabolism*; D000397:Air Pollution; D001234:Aspergillus niger / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D007700:Kinetics; D008031:Lignin / Q000031:analogs & derivatives; D008962:Models, Theoretical; D012336:RNA, Ribosomal, 16S; D012722:Sewage / Q000382:microbiology; D014050:Toluene / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000378:metabolism
chemical_list: D000393:Air Pollutants; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D055549:Volatile Organic Compounds; C507903:coir; D014050:Toluene; D008031:Lignin
keywords: Aspergillus niger; Coir.; Microbial abatement; Toluene