title: Proteomic Alterations in B Lymphocytes of Sensitized Mice in a Model of Chemical-Induced Asthma.
abstract: OBJECTIVE
  The role of B-lymphocytes in chemical-induced asthma is largely unknown. Recent work demonstrated that transferring B lymphocytes from toluene diisocyanate (TDI)-sensitized mice into naïve mice, B cell KO mice and SCID mice, triggered an asthma-like response in these mice after a subsequent TDI-challenge. We applied two-dimensional difference gel electrophoresis (2D-DIGE) to describe the "sensitized signature" of B lymphocytes comparing TDI-sensitized mice with control mice.
  
  
  RESULTS
  Sixteen proteins were identified that were significantly up- or down-regulated in B lymphocytes of sensitized mice. Particularly differences in the expression of cyclophilin A, cofilin 1 and zinc finger containing CCHC domain protein 11 could be correlated to the function of B lymphocytes as initiators of T lymphocyte independent asthma-like responses.
  
  
  CONCLUSIONS
  This study revealed important alterations in the proteome of sensitized B cells in a mouse model of chemical-induced asthma, which will have an important impact on the B cell function.
authors.affiliation: Centre for Environment and Health, KU Leuven, Leuven, Belgium; Research Group Functional Genomics and Proteomics, KU Leuven, Leuven, Belgium.
authors.forename: Steven
authors.initials: S
authors.lastname: Haenen
authors.affiliation: Centre for Environment and Health, KU Leuven, Leuven, Belgium.
authors.forename: Jeroen A J
authors.initials: JA
authors.lastname: Vanoirbeek
authors.affiliation: Centre for Environment and Health, KU Leuven, Leuven, Belgium.
authors.forename: Vanessa
authors.initials: V
authors.lastname: De Vooght
authors.affiliation: Research Group Functional Genomics and Proteomics, KU Leuven, Leuven, Belgium.
authors.forename: Liliane
authors.initials: L
authors.lastname: Schoofs
authors.affiliation: Centre for Environment and Health, KU Leuven, Leuven, Belgium.
authors.forename: Benoit
authors.initials: B
authors.lastname: Nemery
authors.affiliation: Biomedical Research Institute (BIOMED), Hasselt University, Hasselt, Belgium.
authors.forename: Elke
authors.initials: E
authors.lastname: Clynen
authors.affiliation: Centre for Environment and Health, KU Leuven, Leuven, Belgium.
authors.forename: Peter H M
authors.initials: PH
authors.lastname: Hoet
mesh_terms: D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism*; D001402:B-Lymphocytes / Q000378:metabolism*; D008297:Male; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D016513:Mice, SCID; D020543:Proteome / Q000378:metabolism*; D014051:Toluene 2,4-Diisocyanate
chemical_list: D020543:Proteome; D014051:Toluene 2,4-Diisocyanate