title: Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research.
abstract: Engineered DNA-binding molecules such as transcription activator-like effector (TAL or TALE) proteins and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system have been used extensively for genome editing in cells of various types and species. The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can also be utilized for other purposes, such as transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and isolation of chromatin in a locus-specific manner. In this review, we describe applications of these engineered DNA-binding molecules for biological purposes other than genome editing.
authors.affiliation: Chromatin Biochemistry Research Group, Combined Program on Microbiology and Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan. tf1023jp@biken.osaka-u.ac.jp.
authors.forename: Toshitsugu
authors.initials: T
authors.lastname: Fujita
authors.affiliation: Chromatin Biochemistry Research Group, Combined Program on Microbiology and Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan. hodaka@biken.osaka-u.ac.jp.
authors.forename: Hodaka
authors.initials: H
authors.lastname: Fujii
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D064130:CRISPR-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D064113:CRISPR-Cas Systems; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D015202:Protein Engineering; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D064130:CRISPR-Associated Proteins; D004268:DNA-Binding Proteins; D014157:Transcription Factors
keywords: CRISPR/Cas; Cas9; TAL; TALE; dCas9; enChIP; live imaging; locus-specific ChIP; sgRNA; transcription