title: CHR729 Is a CHD3 Protein That Controls Seedling Development in Rice.
abstract: CHD3 is one of the chromatin-remodeling factors that contribute to controlling the expression of genes associated with plant development. Loss-of-function mutants display morphological and growth defects. However, the molecular mechanisms underlying CHD3 regulation of plant development remain unclear. In this study, a rice CHD3 protein, CHR729, was identified. The corresponding mutant line (t483) exhibited late seed germination, low germination rate, dwarfism, low tiller number, root growth inhibition, adaxial albino leaves, and short and narrow leaves. CHR729 encoded a nuclear protein and was expressed in almost all organs. RNA-sequencing analysis showed that several plant hormone-related genes were up- or down-regulated in t483 compared to wild type. In particular, expression of the gibberellin synthetase gibberellin 20 oxidase 4 gene was elevated in the mutant. Endogenous gibberellin assays demonstrated that the content of bioactive GA3 was reduced in t483 compared to wild type. Moreover, the seedling dwarfism, late seed germination, and short root length phenotypes of t483 were partially rescued by treatment with exogenous GA3. These results suggest that the rice CHD3 protein CHR729 plays an important role in many aspects of seedling development and controls this development via the gibberellin pathway.
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Xiaoding
authors.initials: X
authors.lastname: Ma
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Zhai
authors.affiliation: National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Peiyong
authors.initials: P
authors.lastname: Xin
authors.affiliation: National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jinfang
authors.initials: J
authors.lastname: Chu
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Longzhi
authors.initials: L
authors.lastname: Han
mesh_terms: D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology*; D032461:Chromosomes, Plant / Q000502:physiology; D015870:Gene Expression; D018525:Germination; D005875:Gibberellins / Q000378:metabolism; D012275:Oryza / Q000254:growth & development / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D005875:Gibberellins; D010937:Plant Growth Regulators