title: Identification of characteristic TRB V usage in HBV-associated HCC by using differential expression profiling analysis.
abstract: Liver cancer is one of the most common cancers worldwide. CDR3 sequencing-based immune repertoire can be closely associated with cancer prognosis and development. Identifying the specific interaction between the TCR and cellular antigens is important for developing novel immunotherapeutic approaches for the treatment of cancer. The rearranged TCRβ loci amplified using Vβ- and Jβ-specific primers by multi-PCR and sequenced using high-throughput sequencing (HTS) in liver cancers were compared with those of T cells from healthy adult peripheral blood and from adjacent liver tissue. The T-cell repertoires within each tumor show strong similarity to one another but are distinct from those of the circulating T-cell repertoire. In addition, our results demonstrate that there are significant differences in the T-cell repertoires of HCC (hepatocellular carcinoma), ICC (intrahepatic cholangiocarcinoma), and MHC (mixed hepatocellular and cholangiocellular carcinoma). Furthermore, we found that the highly expanded clone (HEC) ratio in blood samples from liver cancer patients differed significantly from those in the blood of healthy adults and hepatitis patients (p< 0.001). The above results suggest that comparison of the T-cell repertoires of tissue and blood could be used to distinguish liver cancer patients from healthy adults and from hepatitis patients. In the future, the diversity of CDR3 sequences in liver cancer may prove to be a useful and novel biomarker for detecting aggressive tumors with high invasive or metastatic capacity.
authors.affiliation: Key Laboratory of Systems Biomedicine (Ministry of Education) and Collaborative Innovation Center of Systems Biomedicine; Shanghai Center for Systems Biomedicine; Shanghai Jiao Tong University; Shanghai, China ; Binhai Genomics Institute; BGI-Tianjin ; Tianjin, China ; Tianjin Translational Genomics Center; BGI-Tianjin ; Tianjin, China.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Han
authors.affiliation: Key Laboratory of Systems Biomedicine (Ministry of Education) and Collaborative Innovation Center of Systems Biomedicine; Shanghai Center for Systems Biomedicine; Shanghai Jiao Tong University; Shanghai, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Binhai Genomics Institute; BGI-Tianjin ; Tianjin, China ; Tianjin Translational Genomics Center; BGI-Tianjin ; Tianjin, China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Binhai Genomics Institute; BGI-Tianjin ; Tianjin, China ; Tianjin Translational Genomics Center; BGI-Tianjin ; Tianjin, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Wu
authors.affiliation: Binhai Genomics Institute; BGI-Tianjin ; Tianjin, China ; Tianjin Translational Genomics Center; BGI-Tianjin ; Tianjin, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Binhai Genomics Institute; BGI-Tianjin ; Tianjin, China ; Tianjin Translational Genomics Center; BGI-Tianjin ; Tianjin, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Li
authors.affiliation: Shenzhen Key Lab. of Infection and Immunity; Shenzhen Third People's Hospital; Guangdong Medical college ; Shenzhen, China.
authors.forename: Xinchun
authors.initials: X
authors.lastname: Chen
authors.affiliation: Shenzhen Key Lab. of Infection and Immunity; Shenzhen Third People's Hospital; Guangdong Medical college ; Shenzhen, China.
authors.forename: Boping
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Systems Biomedicine (Ministry of Education) and Collaborative Innovation Center of Systems Biomedicine; Shanghai Center for Systems Biomedicine; Shanghai Jiao Tong University; Shanghai, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Systems Biomedicine (Ministry of Education) and Collaborative Innovation Center of Systems Biomedicine; Shanghai Center for Systems Biomedicine; Shanghai Jiao Tong University; Shanghai, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ou
authors.affiliation: Binhai Genomics Institute; BGI-Tianjin ; Tianjin, China ; Tianjin Translational Genomics Center; BGI-Tianjin ; Tianjin, China.
authors.forename: Renhua
authors.initials: R
authors.lastname: Wu
authors.affiliation: Tongji Medical College; Huazhong University of Science and Technology ; Wuhan, China.
authors.forename: Wanqiu
authors.initials: W
authors.lastname: Huang
authors.affiliation: Key Laboratory of Systems Biomedicine (Ministry of Education) and Collaborative Innovation Center of Systems Biomedicine; Shanghai Center for Systems Biomedicine; Shanghai Jiao Tong University; Shanghai, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Binhai Genomics Institute; BGI-Tianjin ; Tianjin, China ; Tianjin Translational Genomics Center; BGI-Tianjin ; Tianjin, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Binhai Genomics Institute; BGI-Tianjin ; Tianjin, China ; Tianjin Translational Genomics Center; BGI-Tianjin ; Tianjin, China.
authors.forename: Yinxin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Binhai Genomics Institute; BGI-Tianjin ; Tianjin, China ; Tianjin Translational Genomics Center; BGI-Tianjin ; Tianjin, China.
authors.forename: Dongxing
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Binhai Genomics Institute; BGI-Tianjin ; Tianjin, China ; Tianjin Translational Genomics Center; BGI-Tianjin ; Tianjin, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Binhai Genomics Institute; BGI-Tianjin ; Tianjin, China ; Tianjin Translational Genomics Center; BGI-Tianjin ; Tianjin, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.affiliation: Binhai Genomics Institute; BGI-Tianjin ; Tianjin, China ; Tianjin Translational Genomics Center; BGI-Tianjin ; Tianjin, China ; BGI-Shenzhen ; Shenzhen, China ; Guangzhou Key Laboratory of Cancer Trans-Omics Research (GZ2012, NO348); BGI-Guangzhou , Guangzhou, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yi
authors.affiliation: Department of Nephrology; Xijing Hospital; Fourth Military Medical University ; Xi An, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
authors.affiliation: Key Laboratory of Systems Biomedicine (Ministry of Education) and Collaborative Innovation Center of Systems Biomedicine; Shanghai Center for Systems Biomedicine; Shanghai Jiao Tong University; Shanghai, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
keywords: T cell; TCRβ; hepatocellular carcinoma; high-throughput sequencing; highly expanded clone