title: Anti-Thrombosis Activity of Sinapic Acid Isolated from the Lees of Bokbunja Wine.
abstract: From the lees of bokbunja wine (LBW) made from Rubus coreanus Miquel, we have identified six compounds (1: trans-4-hydroxycinnamic acid; 2: trans-4-hydroxy-3-methoxycinnamic acid; 3: 3,4-dihydroxycinnamic acid; 4: 4-hydroxy-3-methoxybenzoic acid; 5: 3,5-dimethoxy-4- hydroxybenzoic acid; and 6: 3,5-dimethoxy-4-hydroxycinnamic acid (sinapic acid)) through silica gel chromatography and UHPLC-MS. The compounds 1-6 showed strong anticoagulation and platelet aggregation inhibitory activities without hemolytic effect against human red blood cells. To date, this is the first report of the in vitro anti-thrombosis activity of sinapic acid. Our results suggest that different cinnamic and benzoic acid derivatives are closely linked to the anti-thrombosis activity of LBW, and sinapic acid could be developed as a promising anti-thrombosis agent.
authors.affiliation: Department of Food and Nutrition, Andong National University, Andong 760-749, Republic of Korea.
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Research Institute, Kooksoondang Brewery Co, Ltd., Seongnam 460-120, Republic of Korea.
authors.forename: Woo-Chang
authors.initials: WC
authors.lastname: Shin
authors.affiliation: Gyeongsangbukdo Agricultural Research & Extension Services, Daegu 702-708, Republic of Korea.
authors.forename: Dong-Kyoon
authors.initials: DK
authors.lastname: Kang
authors.affiliation: Department of Food and Nutrition, Andong National University, Andong 760-749, Republic of Korea.
authors.forename: Ho-Yong
authors.initials: HY
authors.lastname: Sohn
mesh_terms: D003373:Coumaric Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D012426:Russia; D014920:Wine / Q000032:analysis*
chemical_list: D003373:Coumaric Acids; D005343:Fibrinolytic Agents; C073734:sinapinic acid
keywords: Anti-thrombosis Activity; Rubus coreanus MIQ; Sinapic Acid; lees of bokbunja wine