title: Quantitative high-throughput gene expression profiling of human striatal development to screen stem cell-derived medium spiny neurons.
abstract: A systematic characterization of the spatio-temporal gene expression during human neurodevelopment is essential to understand brain function in both physiological and pathological conditions. In recent years, stem cell technology has provided an in vitro tool to recapitulate human development, permitting also the generation of human models for many diseases. The correct differentiation of human pluripotent stem cell (hPSC) into specific cell types should be evaluated by comparison with specific cells/tissue profiles from the equivalent adult in vivo organ. Here, we define by a quantitative high-throughput gene expression analysis the subset of specific genes of the whole ganglionic eminence (WGE) and adult human striatum. Our results demonstrate that not only the number of specific genes is crucial but also their relative expression levels between brain areas. We next used these gene profiles to characterize the differentiation of hPSCs. Our findings demonstrate a temporal progression of gene expression during striatal differentiation of hPSCs from a WGE toward an adult striatum identity. Present results establish a gene expression profile to qualitatively and quantitatively evaluate the telencephalic hPSC-derived progenitors eventually used for transplantation and mature striatal neurons for disease modeling and drug-screening.
authors.affiliation: Department of Cell Biology, Immunology and Neuroscience, Faculty of Medicine, August Pi i Sunyer Biomedical Research Institute (IDIBAPS), and Networked Biomedical Research Centre for NeuroDegenerative Disorders (CIBERNED), University of Barcelona , Barcelona, Spain.
authors.forename: Marco
authors.initials: M
authors.lastname: Straccia
authors.affiliation: Department of Cell Biology, Immunology and Neuroscience, Faculty of Medicine, August Pi i Sunyer Biomedical Research Institute (IDIBAPS), and Networked Biomedical Research Centre for NeuroDegenerative Disorders (CIBERNED), University of Barcelona , Barcelona, Spain.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Garcia-Diaz Barriga
authors.affiliation: Department of Cell Biology, Immunology and Neuroscience, Faculty of Medicine, August Pi i Sunyer Biomedical Research Institute (IDIBAPS), and Networked Biomedical Research Centre for NeuroDegenerative Disorders (CIBERNED), University of Barcelona , Barcelona, Spain.
authors.forename: Phil
authors.initials: P
authors.lastname: Sanders
authors.affiliation: Department of Cell Biology, Immunology and Neuroscience, Faculty of Medicine, August Pi i Sunyer Biomedical Research Institute (IDIBAPS), and Networked Biomedical Research Centre for NeuroDegenerative Disorders (CIBERNED), University of Barcelona , Barcelona, Spain.
authors.forename: Georgina
authors.initials: G
authors.lastname: Bombau
authors.affiliation: Department of Cell Biology, Immunology and Neuroscience, Faculty of Medicine, August Pi i Sunyer Biomedical Research Institute (IDIBAPS), and Networked Biomedical Research Centre for NeuroDegenerative Disorders (CIBERNED), University of Barcelona , Barcelona, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Carrere
authors.affiliation: Department of Cell Biology, Immunology and Neuroscience, Faculty of Medicine, August Pi i Sunyer Biomedical Research Institute (IDIBAPS), and Networked Biomedical Research Centre for NeuroDegenerative Disorders (CIBERNED), University of Barcelona , Barcelona, Spain.
authors.forename: Pedro Belio
authors.initials: PB
authors.lastname: Mairal
authors.affiliation: Cardiff Repair Group, School of Biosciences and Medicine, Cardiff University , Cardiff, UK.
authors.forename: Ngoc-Nga
authors.initials: NN
authors.lastname: Vinh
authors.affiliation: Cardiff Repair Group, School of Biosciences and Medicine, Cardiff University , Cardiff, UK.
authors.forename: Sun
authors.initials: S
authors.lastname: Yung
authors.affiliation: Cardiff Repair Group, School of Biosciences and Medicine, Cardiff University , Cardiff, UK.
authors.forename: Claire M
authors.initials: CM
authors.lastname: Kelly
authors.affiliation: Regenerative Medicine Institute, Cedars-Sinai Medical Center , Los Angeles, California, USA.
authors.forename: Clive N
authors.initials: CN
authors.lastname: Svendsen
authors.affiliation: Cardiff Repair Group, School of Biosciences and Medicine, Cardiff University , Cardiff, UK.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Kemp
authors.affiliation: CHDI Foundation , Princeton, New Jersey, USA.
authors.forename: Jamshid
authors.initials: J
authors.lastname: Arjomand
authors.affiliation: CHDI Foundation , Princeton, New Jersey, USA.
authors.forename: Ryan C
authors.initials: RC
authors.lastname: Schoenfeld
authors.affiliation: Department of Cell Biology, Immunology and Neuroscience, Faculty of Medicine, August Pi i Sunyer Biomedical Research Institute (IDIBAPS), and Networked Biomedical Research Centre for NeuroDegenerative Disorders (CIBERNED), University of Barcelona , Barcelona, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Alberch
authors.affiliation: Cardiff Repair Group, School of Biosciences and Medicine, Cardiff University , Cardiff, UK.
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Allen
authors.affiliation: Cardiff Repair Group, School of Biosciences and Medicine, Cardiff University , Cardiff, UK.
authors.forename: Anne E
authors.initials: AE
authors.lastname: Rosser
authors.affiliation: Department of Cell Biology, Immunology and Neuroscience, Faculty of Medicine, August Pi i Sunyer Biomedical Research Institute (IDIBAPS), and Networked Biomedical Research Centre for NeuroDegenerative Disorders (CIBERNED), University of Barcelona , Barcelona, Spain.
authors.forename: Josep M
authors.initials: JM
authors.lastname: Canals