title: Porous poly (D,L-lactide-co-glycolide) acid/biosilicate®composite scaffolds for bone tissue engineering.
abstract: This study evaluated the effects of the Biosilicate®and poly (D,L-lactic-co-glycolic) acid composites on bone repair in a tibial bone defect model in rats by means of using histological evaluation (histopathological and morphometric analysis) and gene expression analysis. Eighty male Wistar rats (12 weeks old, weighing ±300 g) were randomly divided into two groups: Biosilicate®group (BG) and Biosilicate®/PLGA group (BG/PLGA). Each group was euthanized at 3, 7, 14, and 21 days after surgery (n = 10 animals per time point). The main findings showed that the incorporation of PLGA into BG had a significant effect on the morphological structure of the material, accelerating mass loss, decreasing the pH and increasing the calcium release. Furthermore, histologic analysis revealed that the BG/PLGA showed increased material degradation, accompanied by higher bone formation compared to BG, after 21 days of implantation. In addition, qRT-PCR analysis showed that BG/PLGA induced an upregulation of the osteogenic genes related to BMP4, Runx2, ALP, and OC. These results show that the present BG/PLGA composite may be used as a bone graft for inducing bone repair. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 63-71, 2017.
authors.affiliation: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, Sao Paulo, Brazil.
authors.forename: Hueliton W
authors.initials: HW
authors.lastname: Kido
authors.affiliation: Department of Physiotherapy, Post-Graduate Program of Biotechnology, Federal University of São Carlos (UFSCar), São Carlos, Sao Paulo, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Brassolatti
authors.affiliation: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, Sao Paulo, Brazil.
authors.forename: Carla R
authors.initials: CR
authors.lastname: Tim
authors.affiliation: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, Sao Paulo, Brazil.
authors.forename: Paulo R
authors.initials: PR
authors.lastname: Gabbai-Armelin
authors.affiliation: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, Sao Paulo, Brazil.
authors.forename: Angela M P
authors.initials: AM
authors.lastname: Magri
authors.affiliation: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, Sao Paulo, Brazil.
authors.forename: Kelly R
authors.initials: KR
authors.lastname: Fernandes
authors.affiliation: Department of Physiotherapy, Post-Graduate Program of Biotechnology, Federal University of São Carlos (UFSCar), São Carlos, Sao Paulo, Brazil.
authors.forename: Paulo S
authors.initials: PS
authors.lastname: Bossini
authors.affiliation: Department of Physiotherapy, Post-Graduate Program of Biotechnology, Federal University of São Carlos (UFSCar), São Carlos, Sao Paulo, Brazil.
authors.forename: Nivaldo A
authors.initials: NA
authors.lastname: Parizotto
authors.affiliation: Department of Materials Engineering, Vitreous Materials Laboratory (LaMaV), Federal University of São Carlos (UFSCar), São Carlos, Sao Paulo, Brazil.
authors.forename: Murilo C
authors.initials: MC
authors.lastname: Crovace
authors.affiliation: Department of Genetics and Evolution, Federal University of São Carlos (UFSCar), São Carlos, Sao Paulo, Brazil.
authors.forename: Iran
authors.initials: I
authors.lastname: Malavazi
authors.affiliation: Department of Genetics and Evolution, Federal University of São Carlos (UFSCar), São Carlos, Sao Paulo, Brazil.
authors.forename: Anderson F
authors.initials: AF
authors.lastname: da Cunha
authors.affiliation: Institute of Chemistry of Sao Carlos, University of São Paulo (USP), São Carlos, Sao Paulo, Brazil.
authors.forename: Ana M G
authors.initials: AM
authors.lastname: Plepis
authors.affiliation: Department of Morphology and Pathology, Federal University of São Carlos (UFSCar), São Carlos, Sao Paulo, Brazil.
authors.forename: Fernanda F
authors.initials: FF
authors.lastname: Anibal
authors.affiliation: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, Sao Paulo, Brazil.
authors.forename: Ana C M
authors.initials: AC
authors.lastname: Rennó
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D005898:Glass / Q000737:chemistry*; D008297:Male; D011098:Polyglactin 910 / Q000737:chemistry / Q000494:pharmacology; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes; C120461:sodium oxide-calcium oxide-silicon dioxide-diphosphorus pentoxide; D011098:Polyglactin 910
keywords: Biosilicate®; PLGA; bone repair; composite; osteogenic genes