title: Differential Inactivation of Fungal Spores in Water and on Seeds by Ozone and Arc Discharge Plasma.
abstract: Seed sterilization is essential for preventing seed borne fungal diseases. Sterilization tools based on physical technologies have recently received much attention. However, available information is very limited in terms of efficiency, safety, and mode of action. In this study, we have examined antifungal activity of ozone and arc discharge plasma, potential tools for seed sterilization. In our results, ozone and arc discharge plasma have shown differential antifungal effects, depending on the environment associated with fungal spores (freely submerged in water or infected seeds). Ozone inactivates Fusarium fujikuroi (fungus causing rice bakanae disease) spores submerged in water more efficiently than arc discharge plasma. However, fungal spores associated with or infecting rice seeds are more effectively deactivated by arc discharge plasma. ROS generated in water by ozone may function as a powerful fungicidal factor. On the other hand, shockwave generated from arc discharge plasma may have greatly contributed to antifungal effects on fungus associated with rice seeds. In support of this notion, addition of ultrasonic wave in ozone generating water has greatly increased the efficiency of seed disinfection.
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, Seoul, 139-701, Republic of Korea.
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Kang
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, Seoul, 139-701, Republic of Korea.
authors.forename: Anchalee
authors.initials: A
authors.lastname: Pengkit
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, Seoul, 139-701, Republic of Korea.
authors.forename: Kihong
authors.initials: K
authors.lastname: Choi
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, Seoul, 139-701, Republic of Korea.
authors.forename: Seong Sil
authors.initials: SS
authors.lastname: Jeon
authors.affiliation: Department of Crop Life Safety, National Academy of Agricultural Science, Suwon, 441-707, Republic of Korea.
authors.forename: Hyo Won
authors.initials: HW
authors.lastname: Choi
authors.affiliation: Department of Crop Environment, National Institute of Crop Science, Suwon, 441-857, Republic of Korea.
authors.forename: Dong Bum
authors.initials: DB
authors.lastname: Shin
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, Seoul, 139-701, Republic of Korea.
authors.forename: Eun Ha
authors.initials: EH
authors.lastname: Choi
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, Seoul, 139-701, Republic of Korea.
authors.forename: Han Sup
authors.initials: HS
authors.lastname: Uhm
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, Seoul, 139-701, Republic of Korea.
authors.forename: Gyungsoon
authors.initials: G
authors.lastname: Park
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002523:Edible Grain / Q000382:microbiology; D005516:Food Microbiology / Q000379:methods; D005670:Fusarium / Q000187:drug effects*; D012275:Oryza / Q000382:microbiology; D010126:Ozone / Q000494:pharmacology*; D058626:Plasma Gases / Q000494:pharmacology*; D012639:Seeds / Q000382:microbiology*; D013172:Spores, Fungal / Q000187:drug effects*; D013242:Sterilization / Q000379:methods; D014871:Water Microbiology
chemical_list: D000935:Antifungal Agents; D058626:Plasma Gases; D010126:Ozone