title: Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates, and Lignins.
abstract: Although like proteins, polysaccharides are synthesized by enzymes, unlike proteins there is no template. This means that they are polydisperse, do not generally have compact folded structures, and are often very large with greater nonideality behavior in solution. This chapter considers the relevant analytical ultracentrifuge methodology available for characterizing these and related carbohydrate-based systems and information this methodology supplies, in terms of sizes, shapes, and interactions using a comprehensive range of examples, including glycoconjugates and lignins. The relevance and potential of recent software developments such as SEDFIT-MSTAR, the Extended Fujita algorithm, and HYDFIT are considered.
authors.affiliation: National Centre for Macromolecular Hydrodynamics, University of Nottingham, Nottingham, United Kingdom. Electronic address: steve.harding@nottingham.ac.uk.
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Harding
authors.affiliation: National Centre for Macromolecular Hydrodynamics, University of Nottingham, Nottingham, United Kingdom; School of Health Sciences, University of Nottingham, Queen's Medical Centre, Nottingham, United Kingdom.
authors.forename: Gary G
authors.initials: GG
authors.lastname: Adams
authors.affiliation: National Centre for Macromolecular Hydrodynamics, University of Nottingham, Nottingham, United Kingdom.
authors.forename: Fahad
authors.initials: F
authors.lastname: Almutairi
authors.affiliation: National Centre for Macromolecular Hydrodynamics, University of Nottingham, Nottingham, United Kingdom.
authors.forename: Qushmua
authors.initials: Q
authors.lastname: Alzahrani
authors.affiliation: National Centre for Macromolecular Hydrodynamics, University of Nottingham, Nottingham, United Kingdom.
authors.forename: Tayyibe
authors.initials: T
authors.lastname: Erten
authors.affiliation: National Centre for Macromolecular Hydrodynamics, University of Nottingham, Nottingham, United Kingdom; Department of Food Engineering, Abant Izzet Baysal University, Bolu, Turkey.
authors.forename: M Samil
authors.initials: MS
authors.lastname: Kök
authors.affiliation: National Centre for Macromolecular Hydrodynamics, University of Nottingham, Nottingham, United Kingdom; School of Health Sciences, University of Nottingham, Queen's Medical Centre, Nottingham, United Kingdom.
authors.forename: Richard B
authors.initials: RB
authors.lastname: Gillis
mesh_terms: D002236:Carbohydrate Conformation; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D006001:Glycoconjugates / Q000737:chemistry / Q000302:isolation & purification*; D008031:Lignin / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D012984:Software; D014461:Ultracentrifugation / Q000379:methods
chemical_list: D006001:Glycoconjugates; D011134:Polysaccharides; D011506:Proteins; D008031:Lignin; D004247:DNA
keywords: Extended Fujita; Flexibility; Interactions; SEDFIT-MSTAR; Shape; Size