title: The role of Kaposi's sarcoma-associated herpesvirus infection in the proliferation of human bladder cancer cells.
abstract: Existing evidence suggests a possible role of viruses in human bladder cancer development. Recently, Kaposi's sarcoma-associated herpesvirus (KSHV) was reported to be the most frequently detected virus in bladder cancer tissue from Croatian patients on screening with the Lawrence Livermore Microbial Detection Array. In the current study, to investigate the functional roles of KSHV in bladder cancer, five bladder cancer cell lines were infected with KSHV and their tumour progression-associated changes investigated. Four KSHV-infected bladder cancer cell lines were established; two invasive bladder cancer cell lines showed higher proliferation rates than uninfected cells. Additionally, these KSHV-infected invasive bladder cancer cells showed a greater number of colonies, which were also significantly larger than those of uninfected cells, in a soft agar colony formation assay. cDNA microarray analysis showed that various genes associated with cell proliferation and cancer development were upregulated in these KSHV-infected bladder cancer cells. Taken together, we suggest that KSHV infection affects the proliferation of a subset of invasive bladder cancer cells and may therefore play a role in their oncogenic progression. Further studies are required to elucidate the exact mechanism used by KSHV to promote bladder cancer progression.
authors.affiliation: Department of Microbiology and Immunology, Eulji University School of Medicine, 143-5 Yongdu-dong, Jung-gu, Daejeon, South Korea.
authors.forename: Jisu
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Microbiology and Immunology, Eulji University School of Medicine, 143-5 Yongdu-dong, Jung-gu, Daejeon, South Korea.
authors.forename: Hyungtaek
authors.initials: H
authors.lastname: Jeon
authors.affiliation: Department of Microbiology and Immunology, Eulji University School of Medicine, 143-5 Yongdu-dong, Jung-gu, Daejeon, South Korea.
authors.forename: Seung-Min
authors.initials: SM
authors.lastname: Yoo
authors.affiliation: Department of Urology, Eulji University Hospital, Eulji University School of Medicine, 1306 Dunsan-dong, Seo-gu, Daejeon, South Korea. jspark.uro@gmail.com.
authors.forename: Jinsung
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Microbiology and Immunology, Eulji University School of Medicine, 143-5 Yongdu-dong, Jung-gu, Daejeon, South Korea. imslee@gmail.com.
authors.forename: Myung-Shin
authors.initials: MS
authors.lastname: Lee
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology*; D006566:Herpesviridae Infections / Q000473:pathology* / Q000821:virology*; D019288:Herpesvirus 8, Human / Q000472:pathogenicity*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D012514:Sarcoma, Kaposi / Q000821:virology*; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000473:pathology* / Q000821:virology*
keywords: Bladder cancer; Human herpesvirus 8; Kaposi’s sarcoma-associated herpesvirus; Microarray; Proliferation