title: Reliable diagnosis of murine type 1 diabetes using a panel of autoantigens and "antigen surrogates" mounted onto a liquid array.
abstract: Autoantibodies raised against β cell antigens are the most reliable preclinical biomarkers for predicting the imminent onset of type 1 diabetes mellitus (T1DM). The most current detection platforms are technically challenging or are run on clinically esoteric equipment. Here, we present a straightforward approach to detect autoantibody biomarkers that employs highly PEGylated microspheres onto which are mounted various capture agents that include affinity-tagged antigens or small molecule "antigen surrogates." After incubation with small quantities of serum, the bound autoantibodies can be measured using a standard flow cytometer. By multiplexing this assay, we show that a panel of antigen and antigen surrogates reliably predicts hyperglycemia in a mouse model of diabetes without false positives.
authors.affiliation: The Scripps Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA. kodadek@scripps.edu.
authors.forename: Todd M
authors.initials: TM
authors.lastname: Doran
authors.forename: Jumpei
authors.initials: J
authors.lastname: Morimoto
authors.forename: Scott
authors.initials: S
authors.lastname: Simanski
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: McEnaney
authors.forename: Thomas
authors.initials: T
authors.lastname: Kodadek
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000276:immunology; D001324:Autoantigens / Q000737:chemistry / Q000276:immunology*; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods*; D003922:Diabetes Mellitus, Type 1 / Q000175:diagnosis* / Q000276:immunology*; D016688:Mice, Inbred NOD; D054852:Small Molecule Libraries
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D054852:Small Molecule Libraries