title: Reproductive physiology of the female Magellanic penguin (Spheniscus magellanicus): Insights from the study of a zoological colony.
abstract: Eight captive female Magellanic penguins (Spheniscus magellanicus) were monitored over a 10week period, commencing at 5weeks prior to egg lay (EL), to increase our understanding of the species' reproductive biology. Females in cordoned nest sites underwent cloacal artificial insemination (AI) every 4-7days with different semen donors for each insemination. The EL interval was 97.9±3.6h (range: 84-108h) and paternity analyses revealed that conceptive inseminations occurred from 11.5 to 4.5days before oviposition. A biphasic pattern of estradiol, testosterone, progesterone and the biochemical analytes triglyceride, iron, calcium and phosphorus occurred in relation to EL, with values increasing (P<0.05) to maximal concentrations during the three weeks preceding oviposition, then decreasing (P<0.05) rapidly after oviposition completion. In comparison with post-lay (baseline) values, concentrations of estradiol and testosterone relative to the first oviposition were elevated at Week-5, and those of triglyceride, a yolk formation index, as well as iron, calcium and phosphorus, became elevated at Week-4 (P<0.05). Collective data indicate an estimated total egg formation interval of 29days, with oviducal transit of the ovulated ovum occurring over the majority of the ∼4day EL interval. These findings indicate that egg formation is prolonged with folliculogenesis initiated at 5weeks or more prior to oviposition. Consequently, the period of folliculogenesis and egg formation is estimated to overlap with the final ∼3weeks that wild females spend at sea prior to returning to land for breeding.
authors.affiliation: SeaWorld and Busch Gardens Reproductive Research Center, SeaWorld Parks and Entertainment, 2595 Ingraham St, San Diego, CA 92109, USA. Electronic address: justine.obrien@seaworld.com.
authors.forename: J K
authors.initials: JK
authors.lastname: O'Brien
authors.affiliation: SeaWorld San Diego, SeaWorld Parks and Entertainment, San Diego, CA 92109, USA.
authors.forename: T L
authors.initials: TL
authors.lastname: Schmitt
authors.affiliation: SeaWorld San Diego, SeaWorld Parks and Entertainment, San Diego, CA 92109, USA.
authors.forename: H H
authors.initials: HH
authors.lastname: Nollens
authors.affiliation: Loyola University Medical Center, Maywood, IL 60153, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Dubach
authors.affiliation: SeaWorld and Busch Gardens Reproductive Research Center, SeaWorld Parks and Entertainment, 2595 Ingraham St, San Diego, CA 92109, USA.
authors.forename: T R
authors.initials: TR
authors.lastname: Robeck
mesh_terms: D000818:Animals; D001947:Breeding; D004958:Estradiol / Q000097:blood; D005260:Female; D010057:Oviducts / Q000502:physiology; D010058:Oviposition / Q000502:physiology*; D010060:Ovulation / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D011374:Progesterone / Q000097:blood; D012098:Reproduction / Q000502:physiology*; D046429:Spheniscidae / Q000502:physiology*; D013739:Testosterone / Q000097:blood
chemical_list: D013739:Testosterone; D011374:Progesterone; D004958:Estradiol
keywords: Artificial insemination; Conservation; Egg production; Penguin; Plasma chemistry; Reproductive biology