title: MicroRNA-23a regulates 3T3-L1 adipocyte differentiation.
abstract: MicroRNAs (miRNAs) are small, non-coding RNAs, which are involved in regulation of a variety of biological processes. Since previous studies regarding the role of miRNAs in the regulation of adipogenic differentiation have shown that miRNA-27a, one member of miRNA-23a∼27a∼24 cluster, could suppress adipogenesis. We now investigated whether miRNA-23a regulates adipogenic differentiation. In the present study, we showed that the expression of miRNA-23a is decreased during the process of adipogenic differentiation. Over-expression of miRNA-23a decreased lipid accumulation and triglyceride content in 3T3-L1 adipocytes. Our results also demonstrated that miRNA-23a decreases mRNA levels of adipocyte-specific genes involved in lipogenic transcription, fatty acid synthesis and fatty acid transport. These findings suggested miRNA-23a to be a new type of adipogenic depressor and to play an important role in regulating adipocyte differentiation.
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Linyuan
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Animal Science, Xichang College, Xichang 615000, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Du
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Luo
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Mingzhou
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Tang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: zhangsh1919@163.com.
authors.forename: Shunhua
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: zhuli7508@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology* / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D035683:MicroRNAs; C534062:Mirn23b microRNA, mouse; D014280:Triglycerides
keywords: 3T3-L1 cell line; Adipocyte differentiation; Adipocyte lipid metabolism; MiRNA-23a