title: The effects of low-intensity He-Ne laser irradiation on erythrocyte metabolism.
abstract: Low-intensity laser irradiation (LILI) can improve the deformability of red blood cells (RBCs). It might be due to the LILI effects on adenosine triphosphate (ATP) level. However, ATP content may not be a valid surrogate marker for RBC deformability. The LILI effects on RBC glycolysis were studied in this paper. Hypertonic RBCs were used in this study. After 5 min irradiation with low-intensity He-Ne laser irradiation (LHNL) at 632.8 nm and 4.4 mW/cm(2), the concentration of intracellular glucose and the activities of phosphofructokinase (PFK) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were measured, respectively. There was no significant change in intracellular glucose concentration. The activity of PFK decreased significantly, but the activity of GAPDH increased significantly. In hypertonic RBCs, LHNL irradiation may decrease the activity of energy-consuming enzymes, but increases the activity of energy-generating enzymes in glycolysis, to improve the RBC deformability.
authors.affiliation: School of Life Sciences, South China Normal University, Guangzhou, 510631, China.
authors.forename: Gang-Yue
authors.initials: GY
authors.lastname: Luo
authors.affiliation: Medical College, Jinan University, Guangzhou, 510632, China.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: Laboratory of Laser Sports Medicine, South China Normal University, University Town, Guangzhou, 510006, China.
authors.forename: En-Xiu
authors.initials: EX
authors.lastname: Wei
authors.affiliation: Department of Otolaryngology, Northwestern University, Chicago, IL, 60611, USA.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Tan
authors.affiliation: Laboratory of Laser Sports Medicine, South China Normal University, University Town, Guangzhou, 510006, China. liutcy@foxmail.com.
authors.forename: Timon Cheng-Yi
authors.initials: TC
authors.lastname: Liu
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D004907:Erythrocyte Deformability / Q000528:radiation effects; D004912:Erythrocytes / Q000378:metabolism* / Q000528:radiation effects*; D005947:Glucose / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006019:Glycolysis / Q000528:radiation effects; D006801:Humans; D054020:Lasers, Gas; D008297:Male; D025221:Phosphofructokinases / Q000378:metabolism
chemical_list: D000255:Adenosine Triphosphate; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D025221:Phosphofructokinases; D005947:Glucose
keywords: Deformability; Glycolysis; Photobiomodulation; Red blood cells