title: Characterisation of seven Inocybe ectomycorrhizal morphotypes from a semiarid woody steppe.
abstract: Ectomycorrhizas (ECM) of Inocybe species (Inocybaceae, Basidiomycota) formed by three host plant species (Populus alba, Salix rosmarinifolia and Pinus nigra) in a semiarid woody steppe of Hungary were studied. To identify the fungal partners, we performed phylogenetic analyses of nucleotide sequences for the internal transcribed spacer region of nuclear DNA (nrDNA ITS) together with sequences gained from public databases. Seven Inocybe ectomycorrhiza morphotypes were morpho-anatomically characterised. Five morphotypes were identified (I. phaeoleuca, I. psammophila, I. semifulva, I. splendens and I. subporospora), whereas two morphotypes represented unidentified Inocybe species. Differences were discernible among the morphotypes, and they showed general anatomical characteristics of Inocybe ECM, such as the slightly organised plectenchymatic mantle (types A, B and E and the gelatinous C). The ECM of I. subporospora and I. phaeoleuca were detected from the introduced Pinus nigra. These two fungi are probably native to the area but capable of forming a novel ectomycorrhizal association with the invasive host.
authors.affiliation: Institute of Biology, Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter sétány 1/c, H-1117, Budapest, Hungary.|Centre for Agricultural Research, Hungarian Academy of Sciences, Plant Protection Institute, Herman Ottó street 15, H-1022, Budapest, Hungary.
authors.forename: Diána
authors.initials: D
authors.lastname: Seress
authors.affiliation: Institute of Biology, Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter sétány 1/c, H-1117, Budapest, Hungary.|Department of Biosciences, Plant Biology, University of Helsinki, P.O. Box 65, FI-00014, Helsinki, Finland.
authors.forename: Bálint
authors.initials: B
authors.lastname: Dima
authors.affiliation: Institute of Biology, Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter sétány 1/c, H-1117, Budapest, Hungary. gmkovacs@ttk.elte.hu.
authors.forename: Gábor M
authors.initials: GM
authors.lastname: Kovács
mesh_terms: D000363:Agaricales / Q000145:classification / Q000166:cytology / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D044822:Biodiversity; D002965:Classification; D004271:DNA, Fungal / Q000235:genetics; D006814:Hungary; D038821:Mycorrhizae / Q000145:classification / Q000166:cytology / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D028223:Pinus / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D032107:Populus / Q000382:microbiology; D032108:Salix / Q000382:microbiology; D014934:Wood / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal
keywords: Diversity; ITS; Indel; Invasive; Molecular taxonomy; Pinus; Species hypotheses