title: Experimental use of crosslinked gelatin glue for arterial hemostasis in cardiovascular surgery.
abstract: BACKGROUND
  Anastomotic needle hole bleeding is a frequently encountered problem in cardiovascular surgeries.
  
  
  OBJECTIVE
  To examine the feasibility of crosslinked gelatin glue as an anastomotic needle hole sealant in comparison with fibrin glue.
  
  
  METHODS
  The in vitro burst water pressures were measured for gelatin and fibrin glue sealed needle holes of expanded polytetrafluoroethylene (ePTFE) or collagen coated woven polyester grafts. For in vivo investigations, abdominal aorta-ePTFE graft anastomoses of heparinized beagle dogs were sealed by gelatin or fibrin glue and hemostatic efficacy was judged. The implanted sites were re-examined 4 weeks postoperatively.
  
  
  RESULTS
  The in vitro burst water pressures of gelatin glue sealed needle holes of both grafts were higher than those sealed by fibrin glue. For in vivo canine studies, hemostasis was successful for all gelatin glue applied suture lines, but not two out of three fibrin glue treated sites when 3-0 polypropylene suture was employed. Although adhesions of surrounding tissues were intense for all sites 4 weeks postoperatively, inflammation was more severe for the fibrin glue group compared to those of gelatin glue.
  
  
  CONCLUSIONS
  Gelatin glue was found to be an effective and safe sealant for accomplishing hemostasis of anastomotic needle holes of vascular grafts.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors.forename: Keigo
authors.initials: K
authors.lastname: Yamashita
authors.affiliation: Biomedical Engineering, School of Medicine, Nara Medical University, Kashihara, Japan.|Queensland Eye Institute, Brisbane, Australia.
authors.forename: Shuko
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors.forename: Nobuoki
authors.initials: N
authors.lastname: Tabayashi
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors.forename: Takehisa
authors.initials: T
authors.lastname: Abe
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hayata
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Hirose
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Hiraga
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Niwa
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Fukuba
authors.affiliation: Diagnostic Pathology, School of Medicine, Nara Medical University, Kashihara, Japan.
authors.forename: Maiko
authors.initials: M
authors.lastname: Takeda
authors.affiliation: Biomedical Engineering, School of Medicine, Nara Medical University, Kashihara, Japan.|Kyoto University, Kyoto, Japan.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Ikada
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Taniguchi
mesh_terms: D000269:Adhesives / Q000008:administration & dosage / Q000737:chemistry; D000818:Animals; D001012:Aorta, Abdominal / Q000293:injuries* / Q000473:pathology; D003432:Cross-Linking Reagents / Q000737:chemistry; D004285:Dogs; D005260:Female; D006489:Hemostatic Techniques; D006490:Hemostatics / Q000008:administration & dosage / Q000138:chemical synthesis; D014014:Tissue Adhesives / Q000008:administration & dosage* / Q000138:chemical synthesis*; D016896:Treatment Outcome; D014656:Vascular Surgical Procedures / Q000009:adverse effects*; D057772:Vascular System Injuries / Q000209:etiology* / Q000473:pathology / Q000628:therapy*
chemical_list: D000269:Adhesives; D003432:Cross-Linking Reagents; D006490:Hemostatics; D014014:Tissue Adhesives
keywords: Gelatin; fibrin glue; needle hole hemostasis; sealant