title: Beneficial effects of protease preparations derived fromAspergilluson the colonic luminal environment in rats consuming a high-fat diet.
abstract: The present study investigated the effects of the dietary addition of the protease preparations derived fromAspergilluson the colonic luminal environment. Rats were fed a 30% beef tallow diet with or without the protease preparations, including Amano protease (protease A 'Amano SD', neutral proteases fromAspergillusspp.) or orientase (orientase AY, acid proteases fromAspergillus niger) at the dose of 0.2% for 3 weeks. CecalBifidobacteriumwas significantly elevated in the dietary Amano protease group (194-fold, P<0.05), but not in the orientase group.Lactobacilluswas elevated in the two groups (P<0.05). Cecaln-butyrate, propionate and lactate were higher in the Amano protease and orientase groups compared with the controls (P<0.05). Fecal immunoglobulin A and mucins were elevated in the Amano protease group (P<0.05). These results suggest the potential effect of the consumption ofAspergillus-derived protease preparations that are favorable for the colonic luminal environment in rats fed a high-fat diet.
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
authors.forename: Yongshou
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
authors.forename: Novita Vivi
authors.initials: NV
authors.lastname: Sitanggang
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
authors.forename: Norihisa
authors.initials: N
authors.lastname: Kato
authors.affiliation: Ahjikan Co., Ltd., Hiroshima 733-8677, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Inoue
authors.affiliation: Ahjikan Co., Ltd., Hiroshima 733-8677, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Murakami
authors.affiliation: Technology Development Laboratory, Yaegaki Bio-Industry Incorporated, Himeji 679-4298, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Watanabe
authors.affiliation: Technology Development Laboratory, Yaegaki Bio-Industry Incorporated, Himeji 679-4298, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Iguchi
authors.affiliation: Faculty of Human Life Sciences, Fuji Women's University, Ishikari, Hokkaido 061-3204, Japan.
authors.forename: Yukako
authors.initials: Y
authors.lastname: Okazaki
keywords: Aspergillus-derived proteases; immunoglobulin A; intestinal microflora; mucins; organic acids