title: From Uniplex to Multiplex Molecular Profiling in Advanced Non-Small Cell Lung Carcinoma.
abstract: Non-small cell lung carcinoma is a leading cause of cancer death worldwide. Understanding the molecular biology of survival and proliferation of cancer cells led to a new molecular classification of lung cancer and the development of targeted therapies with promising results. With the advances of image-guided biopsy techniques, tumor samples are becoming smaller, and the molecular testing techniques have to overcome the challenge of integrating the characterization of a panel of abnormalities including gene mutations, copy-number changes, and fusions in a reduced number of assays using only a small amount of genetic material. This article reviews the current knowledge about the most frequent actionable molecular abnormalities in non-small cell lung carcinoma, the new approaches of molecular analysis, and the implications of these findings in the context of clinical practice.
authors.affiliation: From the Department of Translational Molecular Pathology, The University of Texas, MD Anderson Cancer Center, Houston, TX.
authors.forename: Ecaterina E
authors.initials: EE
authors.lastname: Ileana
authors.forename: Ignacio I
authors.initials: II
authors.lastname: Wistuba
authors.forename: Julie G
authors.initials: JG
authors.lastname: Izzo
mesh_terms: D014408:Biomarkers, Tumor; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism* / Q000628:therapy; D019468:Disease Management; D044127:Epigenesis, Genetic; D020869:Gene Expression Profiling / Q000379:methods; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D023281:Genomics / Q000379:methods; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism* / Q000628:therapy; D040901:Proteomics / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor