title: Early serodiagnosis of trichinellosis by ELISA using excretory-secretory antigens of Trichinella spiralis adult worms.
abstract: BACKGROUND
  The excretory-secretory (ES) antigens of Trichinella spiralis muscle larvae (ML) are the most commonly used diagnostic antigens for trichinellosis. Their main disadvantage for the detection of anti-Trichinella IgG is false-negative results during the early stage of infection. Additionally, there is an obvious window between clinical symptoms and positive serology.
  
  
  METHODS
  ELISA with adult worm (AW) ES antigens was used to detect anti-Trichinella IgG in the sera of experimentally infected mice and patients with trichinellosis. The sensitivity and specificity were compared with ELISAs with AW crude antigens and ML ES antigens.
  
  
  RESULTS
  In mice infected with 100 ML, anti-Trichinella IgG were first detected by ELISA with the AW ES antigens, crude antigens and ML ES antigens 8, 12 and 12 days post-infection (dpi), respectively. In mice infected with 500 ML, specific antibodies were first detected by ELISA with the three antigen preparations at 10, 8 and 10 dpi, respectively. The sensitivity of the ELISA with the three antigen preparations for the detection of sera from patients with trichinellosis at 35 dpi was 100%. However, when the patients' sera were collected at 19 dpi, the sensitivities of the ELISAs with the three antigen preparations were 100% (20/20), 100% (20/20) and 75% (15/20), respectively (P < 0.05). The specificities of the ELISAs with the three antigen preparations were 98.11, 95.60 and 89.31%, respectively (P < 0.05).
  
  
  CONCLUSIONS
  The sensitivity and specificity of the T. spiralis AW ES antigens were superior to those of the AW crude antigens and ML ES antigens. Thus, the AW ES antigens might serve as potential antigens for the early and specific serodiagnosis of trichinellosis.
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou, China. sungege312216@163.com.
authors.forename: Ge-Ge
authors.initials: GG
authors.lastname: Sun
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou, China. wangzq@zzu.edu.cn.
authors.forename: Zhong-Quan
authors.initials: ZQ
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou, China. liuchunying0302@126.com.
authors.forename: Chun-Ying
authors.initials: CY
authors.lastname: Liu
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou, China. jpdaisy@126.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou, China. liuruodan2006@126.com.
authors.forename: Ruo-Dan
authors.initials: RD
authors.lastname: Liu
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou, China. wenhui124w@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Wen
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou, China. qixin0000@foxmail.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou, China. x103001wangli@126.com.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou, China. cuij@zzu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Cui
mesh_terms: D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D042241:Early Diagnosis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015801:Helminth Proteins / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D007074:Immunoglobulin G; C097419:excretory-secretory antigen, Trichinella