title: A new enzyme-free quadratic SERS signal amplification approach for circulating microRNA detection in human serum.
abstract: In this communication, we proposed a new enzyme-free quadratic SERS signal amplification approach for the ultrasensitive detection of circulating miRNA in human serum. Combined with miRNA-triggered hybridization chain reaction and Ag(+)-mediated cascade amplification, a limit of miRNA detection as low as 0.3 fM could be achieved. More importantly, our method is suitable for the direct detection of circulating miRNAs in human serum collected from patients with different stages of chronic lymphocytic leukemia (CLL).
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. Yangrh@pku.ed.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. Yangrh@pku.ed.cn.
authors.forename: Dandan
authors.initials: D
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. Yangrh@pku.ed.cn.
authors.forename: Muling
authors.initials: M
authors.lastname: Shi
authors.affiliation: School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha, 410004, China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. Yangrh@pku.ed.cn.
authors.forename: Yinhui
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. Yangrh@pku.ed.cn and School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha, 410004, China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Yang
mesh_terms: D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood*; D035683:MicroRNAs / Q000097:blood*; D009693:Nucleic Acid Hybridization; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
chemical_list: D035683:MicroRNAs