title: Characteristics and antioxidant of Ulva intestinalis sulphated polysaccharides extracted with different solvents.
abstract: Ulva intestinalis, a tubular green seaweed, is a rich source of nutrient, especially sulphated polysaccharides. Sulphated polysaccharides from U. intestinalis were extracted with distilled water, 0.1N HCl, and 0.1N NaOH at 80°C for 1, 3, 6, 12, and 24h to study the effect of the extraction solvent and time on their chemical composition and antioxidant activity. Different types of solvents and extraction time had a significant influence on the chemical characteristics and antioxidant activity (p<0.05). Monosaccharide composition and FT-IR spectra analyses revealed that sulphated polysaccharides from all solvent extractions have a typical sugar backbone (glucose, rhamnose, and sulphate attached at C-2 or C-3 of rhamnose). Sulphated polysaccharides extracted with acid exhibited greater antioxidant activity than did those extracted with distilled water and alkali. The results indicated that solvent extraction could be an efficacious method for enhancing antioxidant activity by distinct molecular weight and chemical characteristic of sulphated polysaccharides.
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tientalay 25 Rd., Thakham, Bangkhuntien, Bangkok 10150, Thailand.
authors.forename: Napassorn
authors.initials: N
authors.lastname: Peasura
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tientalay 25 Rd., Thakham, Bangkhuntien, Bangkok 10150, Thailand. Electronic address: nutta.lao@kmutt.ac.th.
authors.forename: Natta
authors.initials: N
authors.lastname: Laohakunjit
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tientalay 25 Rd., Thakham, Bangkhuntien, Bangkok 10150, Thailand.
authors.forename: Orapin
authors.initials: O
authors.lastname: Kerdchoechuen
authors.affiliation: Thailand Institute of Scientific and Technological Research, 35 Mu 3 Tambon Khlong Ha, Amphoe Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Sorada
authors.initials: S
authors.lastname: Wanlapa
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002850:Chromatography, Gel; D016166:Free Radical Scavengers / Q000737:chemistry; D006851:Hydrochloric Acid / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D011506:Proteins / Q000032:analysis; D012972:Sodium Hydroxide / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013431:Sulfates / Q000032:analysis / Q000494:pharmacology*; D013451:Sulfonic Acids / Q000737:chemistry; D044445:Ulva / Q000737:chemistry*; D014867:Water / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D009005:Monosaccharides; D010851:Picrates; D011134:Polysaccharides; D011506:Proteins; D012997:Solvents; D013431:Sulfates; D013451:Sulfonic Acids; D014867:Water; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D017665:Hydroxyl Radical; D012972:Sodium Hydroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D006851:Hydrochloric Acid
keywords: Antioxidant activity; Characterization; Solvent extraction; Sulfated polysaccharide; Ulva intestinalis