title: From gene to biorefinery: microbial β-etherases as promising biocatalysts for lignin valorization.
abstract: The set-up of biorefineries for the valorization of lignocellulosic biomass will be core in the future to reach sustainability targets. In this area, biomass-degrading enzymes are attracting significant research interest for their potential in the production of chemicals and biofuels from renewable feedstock. Glutathione-dependent β-etherases are emerging enzymes for the biocatalytic depolymerization of lignin, a heterogeneous aromatic polymer abundant in nature. They selectively catalyze the reductive cleavage of β-O-4 aryl-ether bonds which account for 45-60% of linkages present in lignin. Hence, application of β-etherases in lignin depolymerization would enable a specific lignin breakdown, selectively yielding (valuable) low-molecular-mass aromatics. Albeit β-etherases have been biochemically known for decades, only very recently novel β-etherases have been identified and thoroughly characterized for lignin valorization, expanding the enzyme toolbox for efficient β-O-4 aryl-ether bond cleavage. Given their emerging importance and potential, this mini-review discusses recent developments in the field of β-etherase biocatalysis covering all aspects from enzyme identification to biocatalytic applications with real lignin samples.
authors.affiliation: Institute of Biotechnology, RWTH Aachen University , Aachen, Germany.
authors.forename: Pere
authors.initials: P
authors.lastname: Picart
authors.affiliation: Sustainable Momentum, SL., Las Palmas de Gran Canaria , Spain.
authors.forename: Pablo Domínguez
authors.initials: PD
authors.lastname: de María
authors.affiliation: Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig , Braunschweig, Germany.
authors.forename: Anett
authors.initials: A
authors.lastname: Schallmey
keywords: biocatalysis; biomass utilization; biorefineries; lignin; β-arylether cleavage; β-etherases