title: Exogenous Nitric Oxide (NO) Interferes with Lead (Pb)-Induced Toxicity by Detoxifying Reactive Oxygen Species in Hydroponically Grown Wheat (Triticum aestivum) Roots.
abstract: Nitric Oxide (NO) is a bioactive signaling molecule that mediates a variety of biotic and abiotic stresses. The present study investigated the role of NO (as SNP [sodium nitroprusside]) in ameliorating lead (Pb)-toxicity in Triticum aestivum (wheat) roots. Pb (50 and 250 μM) alone and in combination with SNP (100 μM) was given to hydroponically grown wheat roots for a period of 0-8 h. NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant. NO ameliorated Pb-induced membrane damage in wheat roots as evidenced by decreased ion-leakage and in situ histochemical localization. Pb-exposure significantly decreased in vivo NO level. The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.
authors.affiliation: Department of Environment Studies, Panjab University, Chandigarh, 160 014, India.
authors.forename: Gurpreet
authors.initials: G
authors.lastname: Kaur
authors.affiliation: Department of Environment Studies, Panjab University, Chandigarh, 160 014, India.
authors.forename: Harminder Pal
authors.initials: HP
authors.lastname: Singh
authors.affiliation: Department of Botany, Panjab University, Chandigarh, 160 014, India.
authors.forename: Daizy R
authors.initials: DR
authors.lastname: Batish
authors.affiliation: Department of Botany, Panjab University, Chandigarh, 160 014, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Mahajan
authors.affiliation: Department of Botany, Panjab University, Chandigarh, 160 014, India.
authors.forename: Ravinder Kumar
authors.initials: RK
authors.lastname: Kohli
authors.affiliation: Chandigarh College of Engineering and Technology, Chandigarh-160019, India.
authors.forename: Valbha
authors.initials: V
authors.lastname: Rishi
mesh_terms: D002463:Cell Membrane Permeability / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D006861:Hydrogen Peroxide / Q000378:metabolism; D018527:Hydroponics; D007854:Lead / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D009569:Nitric Oxide / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development; D014908:Triticum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D009599:Nitroprusside; D007854:Lead; D009569:Nitric Oxide; D006861:Hydrogen Peroxide