title: Determination of 11 quinolones in bovine milk using immunoaffinity stir bar sorptive microextraction and liquid chromatography with fluorescence detection.
abstract: A sensitive, selective and reproducible immunoaffinity stir bar sorptive microextraction (SBSME) coupled with liquid chromatography-fluorescence method for determination of 11 quinolones (QNs) in bovine milk was developed and validated. It is first report of a broad-specificity monoclonal antibody to QNs that has been immobilized to glass bar for preparation of a re-usable immunoaffinity stir bar. Analytes were extracted by placing stir bar in milk and shaking on a rotary shaker for 30min at 30rpm, followed by liquid chromatography and fluorescence detection. The newly developed method has limits of detection for each QN from 0.05 to 0.1ng/g with intra-day and inter-day precision ranging from 3.2 to 11.9% and from 5.2 to 12.5%, respectively. This allowed us to quantitatively analyze drugs in bovine milk with the advantage of significantly simplified sample preparation. The proposed method was successfully applied to the bovine milk samples analyses with QNs, demonstrating its rare application in animal food safety analysis.
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384 Tianjin, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yao
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384 Tianjin, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384 Tianjin, People's Republic of China.
authors.forename: Linyan
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384 Tianjin, People's Republic of China.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Gong
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384 Tianjin, People's Republic of China.
authors.forename: Liuan
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384 Tianjin, People's Republic of China.
authors.forename: Tianming
authors.initials: T
authors.lastname: Jin
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384 Tianjin, People's Republic of China. Electronic address: hhlicun@163.com.
authors.forename: Cun
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D007163:Immunosorbent Techniques; D008892:Milk / Q000737:chemistry*; D015363:Quinolones / Q000032:analysis / Q000302:isolation & purification*; D052617:Solid Phase Microextraction / Q000379:methods*
chemical_list: D015363:Quinolones
keywords: Bovine milk; Immunoaffinity stir bar; Liquid chromatography; Monoclonal antibody; Quinolone