title: A Ca-alginate particle co-immobilized with Phanerochaete chrysosporium cells and the combined cross-linked enzyme aggregates from Trametes versicolor.
abstract: For improving stability of immobilized white-rot fungus to treat various effluents, Phanerochaete chrysosporium cells and the combined cross-link enzyme aggregates (combi-CLEAs) prepared from Trametes versicolor were co-immobilized into the Ca-alginate gel particles in this paper. The activity yields of obtained combi-CLEAs were 42.7% for lignin peroxidases (LiPs), 31.4% for manganese peroxidases (MnPs) and 40.4% for laccase (Lac), respectively. And their specific activities were 30.2U/g as combi-CLEAs-LiPs, 9.5 U/g as combi-CLEAs-MnPs and 28.4 U/g as combi-CLEAs-Lac. Further, the present of the combi-CLEAs in the particles extremely improved their ability to degrade the dyes. Compared to the immobilized Ph. chrysosporium without the combi-CLEAs, the co-immobilized particles enhanced the decolorized rate of Acid Violet 7 (from 45.2% to 93.4%) and Basic Fuchsin (from 12.1% to 67.9%). In addition, the addition of the combi-CLEAs improved the adaptability of the white-rot fungal particles to adverse environmental conditions.
authors.affiliation: Qilu University of Technology, No. 3501, Daxue Rd, Changqing District, Jinan, Shandong Province 250353, China. Electronic address: lyc@qlu.edu.cn.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Qilu University of Technology, No. 3501, Daxue Rd, Changqing District, Jinan, Shandong Province 250353, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Qilu University of Technology, No. 3501, Daxue Rd, Changqing District, Jinan, Shandong Province 250353, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Xu
authors.affiliation: Qilu University of Technology, No. 3501, Daxue Rd, Changqing District, Jinan, Shandong Province 250353, China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Jin
mesh_terms: D000464:Alginates / Q000378:metabolism*; D001391:Azo Compounds / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D042845:Laccase / Q000378:metabolism; D009282:Naphthalenesulfonates / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D020075:Phanerochaete / Q000378:metabolism*; D055454:Trametes / Q000201:enzymology*
chemical_list: D000464:Alginates; D001391:Azo Compounds; D004396:Coloring Agents; D006603:Hexuronic Acids; D009282:Naphthalenesulfonates; D020723:Glucuronic Acid; D042845:Laccase; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; C551247:acid violet 7
keywords: Co-immobilization; Combi-CLEAs; Dye; Heavy metal ionic; White-rot fungi