title: Complete genome sequence of Clostridium pasteurianum NRRL B-598, a non-type strain producing butanol.
abstract: The strain Clostridium pasteurianum NRRL B-598 is non-type, oxygen tolerant, spore-forming, mesophilic and heterofermentative strain with high hydrogen production and ability of acetone-butanol fermentation (ethanol production being negligible). Here, we present the annotated complete genome sequence of this bacterium, replacing the previous draft genome assembly. The genome consisting of a single circular 6,186,879 bp chromosome with no plasmid was determined using PacBio RSII and Roche 454 sequencing.
authors.affiliation: Department of Biomedical Engineering, Brno University of Technology, Technicka 12, 616 00 Brno, Czech Republic. Electronic address: sedlar@feec.vutbr.cz.
authors.forename: Karel
authors.initials: K
authors.lastname: Sedlar
authors.affiliation: Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czech Republic. Electronic address: jan.kolek@vscht.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Kolek
authors.affiliation: Department of Biomedical Engineering, Brno University of Technology, Technicka 12, 616 00 Brno, Czech Republic. Electronic address: skutkova@feec.vutbr.cz.
authors.forename: Helena
authors.initials: H
authors.lastname: Skutkova
authors.affiliation: Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czech Republic. Electronic address: barbora.branska@vscht.cz.
authors.forename: Barbora
authors.initials: B
authors.lastname: Branska
authors.affiliation: Department of Biomedical Engineering, Brno University of Technology, Technicka 12, 616 00 Brno, Czech Republic. Electronic address: provaznik@feec.vutbr.cz.
authors.forename: Ivo
authors.initials: I
authors.lastname: Provaznik
authors.affiliation: Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czech Republic. Electronic address: petra.patakova@vscht.cz.
authors.forename: Petra
authors.initials: P
authors.lastname: Patakova
mesh_terms: D000440:Butanols / Q000378:metabolism*; D003013:Clostridium / Q000235:genetics* / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D000440:Butanols; D004269:DNA, Bacterial
keywords: Butanol; Clostridium pasteurianum; Genome; Oxygen tolerance; Pyrosequencing; Single molecule real-time sequencing