title: Immunohistochemical Analysis of MMP-2 Expression in the Myocardium During the Postinfarction Period.
abstract: Immunohistochemical analysis revealed 2.5-fold increased of expression MMP-2 in myocardium samples during the early period (up to 3 days) of postinfarction reparative regeneration. During this period, MMP-2 was detected mainly in monocytes/macrophages circulating in the blood and migrating to the necrotic zone, while expression in the intermuscular and perivascular connective tissue was lower. At later terms, with development of large focal and diffuse cardiosclerosis, MMP-2 expression significantly decreased (to the initial level) and was detected mainly in the foci of intermuscular and perivascular fibrosis, its area in the sections increased by 1.8 times. Evaluation of MMP-2 expression in the blood vessels showed that the immunohistochemical reaction was the most pronounced in the walls of new sinusoidal vessels and the minimum in the intramural arteries of medium diameter. These results attest to an important role of MMP in connective tissue remodeling (proteolytic degradation) during the early period of postinfarction reparative regeneration. The decrease in MMP-2 expression observed at later terms correlated with myocardial fibrosis progression.
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia. pathol@inbox.ru.
authors.forename: L M
authors.initials: LM
authors.lastname: Nepomnyashchikh
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
authors.forename: E L
authors.initials: EL
authors.lastname: Lushnikova
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Bakarev
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
authors.forename: D B
authors.initials: DB
authors.lastname: Nikityuk
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
authors.forename: E I
authors.initials: EI
authors.lastname: Yuzhik
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Mzhelskaya
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
authors.forename: R D
authors.initials: RD
authors.lastname: Nepomnyashchikh
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
authors.forename: M G
authors.initials: MG
authors.lastname: Klinnikova
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Karpova
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001808:Blood Vessels / Q000201:enzymology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D009203:Myocardial Infarction / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D009928:Organ Specificity
chemical_list: C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2
keywords: cardiofi brosis; immunohistochemistry; matrix metalloproteinase-2; myocardial infarction