title: Ultrasound-assisted green solvent extraction of high-added value compounds from microalgae Nannochloropsis spp.
abstract: The aim of this work was to investigate ultrasound (US)-assisted green solvent extraction of valuable compounds from the microalgae Nannochloropsis spp. Individual green solvents (water, ethanol (EtOH), dimethyl sulfoxide (DMSO)) and binary mixture of solvents (water-DMSO and water-EtOH) were used for the extraction procedures. Maximum total phenolic compounds yield (Yp ≈ 0.33) was obtained after US pre-treatment (W=400 W, 15 min), being almost 5-folds higher compared to that found for the untreated samples and aqueous extraction (Yp ≈ 0.06). The highest yield of total chlorophylls (Yc ≈ 0.043) was obtained after US (W=400 W, 7.5 min), being more than 9-folds higher than those obtained for the untreated samples and aqueous extraction (Yc ≈ 0.004). The recovery efficiency decreased as DMSO>EtOH>H2O. The optimal conditions to recover phenolic compounds and chlorophylls from microalgae were obtained after US pre-treatment (400 W, 5 min), binary mixtures of solvents (water-DMSO and water-EtOH) at 25-30%, and microalgae concentration of 10%.
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, Laboratoire Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR), Centre de Recherche de Royallieu, B.P. 20529, 60205 Compiègne Cedex, France; Institute of Biocolloidal Chemistry named after F. D. Ovcharenko, NAS of Ukraine, 42, Blvr. Vernadskogo, Kyiv 03142, Ukraine.
authors.forename: O
authors.initials: O
authors.lastname: Parniakov
authors.affiliation: Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132 Fisciano (SA), Italy.
authors.forename: E
authors.initials: E
authors.lastname: Apicella
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, Laboratoire Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR), Centre de Recherche de Royallieu, B.P. 20529, 60205 Compiègne Cedex, France.
authors.forename: M
authors.initials: M
authors.lastname: Koubaa
authors.affiliation: Nutrition and Food Science Area, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, s/n 46100 Burjassot, València, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Barba
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, Laboratoire Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR), Centre de Recherche de Royallieu, B.P. 20529, 60205 Compiègne Cedex, France. Electronic address: nabil.grimi@utc.fr.
authors.forename: N
authors.initials: N
authors.lastname: Grimi
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, Laboratoire Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR), Centre de Recherche de Royallieu, B.P. 20529, 60205 Compiègne Cedex, France; Institute of Biocolloidal Chemistry named after F. D. Ovcharenko, NAS of Ukraine, 42, Blvr. Vernadskogo, Kyiv 03142, Ukraine.
authors.forename: N
authors.initials: N
authors.lastname: Lebovka
authors.affiliation: Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132 Fisciano (SA), Italy.
authors.forename: G
authors.initials: G
authors.lastname: Pataro
authors.affiliation: Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132 Fisciano (SA), Italy; ProdAl Scarl, Via Ponte don Melillo, Fisciano (SA), Italy.
authors.forename: G
authors.initials: G
authors.lastname: Ferrari
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, Laboratoire Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR), Centre de Recherche de Royallieu, B.P. 20529, 60205 Compiègne Cedex, France.
authors.forename: E
authors.initials: E
authors.lastname: Vorobiev
mesh_terms: D002734:Chlorophyll / Q000032:analysis*; D004121:Dimethyl Sulfoxide; D000431:Ethanol; D058086:Microalgae / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D012997:Solvents; D014465:Ultrasonics
chemical_list: D010636:Phenols; D012997:Solvents; D002734:Chlorophyll; D000431:Ethanol; D004121:Dimethyl Sulfoxide
keywords: Chlorophylls; Microalgae; Nannochloropsis; Phenolic compounds; Ultrasound-assisted extraction