title: Antitumor and immunomodulatory activity of genkwanin on colorectal cancer in the APC(Min/+) mice.
abstract: Colorectal cancer is the third most common malignant tumor with high morbidity and mortality. To evaluate the antitumor effect of genkwanin on colorectal cancer enhanced by western high-fat diet, we investigated the activity of genkwanin on HT-29 and SW-480 human colorectal cancer lines in vitro and on the APC(Min/+) mice in vivo. In a cell culture system, six different inflammatory cytokines obviously stimulated two cancer cells growth in a concentration-dependent manner, while genkwanin significantly inhibited HT-29 and SW-480 human colorectal cancer cells proliferation and inflammatory cytokine IL-8 secretion. In the APC(Min/+) mice, the body weights, spleen and thymus indexes and immunity cytokine secretions were significantly improved after oral administration 12.5 and 25mg/kg/day of genkwanin. Besides, the tumor multiplicity changes and inflammatory cytokine levels were markedly reduced in two genkwanin-treated groups. The dysplastic adenomatous changes were also obviously ameliorated in gut histopathology. Taken together, our results indicated that genkwanin had a better antitumor activity partly via enhancing host immunity and decreasing the inflammatory cytokine levels. Genkwanin may be an effective chemotherapeutic agent for the treatment of colorectal cancer.
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Zi-Jing
authors.initials: ZJ
authors.lastname: Song
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210000, China.
authors.forename: Xin
authors.initials: X
authors.lastname: He
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Run-Qi
authors.initials: RQ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; Tang Center of Herbal Medicine Research, Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL 60637, USA. Electronic address: zhangchunfeng67@163.com.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: lifeicpu@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: Tang Center of Herbal Medicine Research, Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Chong-Zhi
authors.initials: CZ
authors.lastname: Wang
authors.affiliation: Tang Center of Herbal Medicine Research, Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Chun-Su
authors.initials: CS
authors.lastname: Yuan
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000188:drug therapy* / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000627:therapeutic use*; D001835:Body Weight; D049109:Cell Proliferation; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects; D005260:Female; D047309:Flavones / Q000737:chemistry / Q000627:therapeutic use*; D019073:HT29 Cells; D006801:Humans; D008297:Male; D051379:Mice; D015394:Molecular Structure
chemical_list: D000970:Antineoplastic Agents; D016207:Cytokines; D004041:Dietary Fats; D047309:Flavones; C014568:genkwanin
keywords: APC(Min/+) mice; Colorectal cancer; Genkwanin; Immunity organ index; Inflammatory cytokines