title: Suppression of ITGB4 Gene Expression in PC-3 Cells with Short Interfering RNA Induces Changes in the Expression of β-Integrins Associated with RGD-Receptors.
abstract: We studied the effect of transfection of PC-3 prostate cancer cells with a plasmid encoding shRNA complimentary to a fragment of integrin β4 (ITGB4). The results attest to considerable changes in the transcriptome of transfected cells. For instance, compensatory changes in the expression of integrin family genes were found.
authors.affiliation: BioClinicum Research and Technical Centre, Moscow, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Knyazev
authors.affiliation: Federal Medical Research Center, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: K M
authors.initials: KM
authors.lastname: Nyushko
authors.affiliation: Federal Medical Research Center, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: B Ya
authors.initials: BY
authors.lastname: Alekseev
authors.affiliation: BioClinicum Research and Technical Centre, Moscow, Russia.
authors.forename: T R
authors.initials: TR
authors.lastname: Samatov
authors.affiliation: BioClinicum Research and Technical Centre, Moscow, Russia. mshkurnikov@gmail.com.|Federal Medical Research Center, Ministry of Health of the Russian Federation, Moscow, Russia. mshkurnikov@gmail.com.
authors.forename: M Yu
authors.initials: MY
authors.lastname: Shkurnikov
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D039641:Integrin beta Chains / Q000235:genetics / Q000378:metabolism; D039663:Integrin beta4 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D011471:Prostatic Neoplasms; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D011971:Receptors, Immunologic / Q000378:metabolism*; D018000:Receptors, Peptide / Q000378:metabolism*
chemical_list: C059834:ITGB4 protein, human; D039641:Integrin beta Chains; D039663:Integrin beta4; D034741:RNA, Small Interfering; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; C051382:arginyl-glycyl-aspartic acid directed cell adhesion receptor
keywords: ITGB4; RNA interference; integrin; metastasizing; prostate cancer