title: Genotoxicity assessment of cobalt chloride in Eisenia hortensis earthworms coelomocytes by comet assay and micronucleus test.
abstract: Cobalt and its different compounds are extensively used worldwide and considered as possible environmental pollutant. Earthworms are useful model organism and its different species are used to monitor soil pollution. No study has been found to detect cobalt chloride (CoCl2) genotoxicity in earthworms. So, current study aimed to evaluate CoCl2 induced genotoxicity in Eisenia hortensis earthworms coelomocytes by alkaline comet assay (CA) and micronucleus (MN) test. The earthworms (n = 10 for each group) were exposed to different series of CoCl2 concentrations (100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm, 600 ppm) to find LD50. The LD50 for CoCl2 was found at 226 ppm. Then, doses of LD50/2, LD50 and 2XLD50 for 48 h were used. CA and MN demonstrated the significant increase (P < 0.05) in DNA damage and chromosomal aberrations. Dose dependent relationship was found. Highest DNA damage and chromosomal aberrations were noticed at 2XLD50. The results concluded that CoCl2 induced DNA damage, cytokinesis failure and chromosomal aberrations in E. hortensis earthworms.
authors.affiliation: Afyon Kocatepe University, Faculty of Science and Literatures, Molecular Biology and Genetic Department, 03200, Afyonkarahisar, Turkey. Electronic address: cigerci@aku.edu.tr.
authors.forename: İbrahim Hakkı
authors.initials: İH
authors.lastname: Ciğerci
authors.affiliation: Afyon Kocatepe University, Faculty of Science and Literatures, Molecular Biology and Genetic Department, 03200, Afyonkarahisar, Turkey.
authors.forename: Muhammad Muddassir
authors.initials: MM
authors.lastname: Ali
authors.affiliation: Afyon Kocatepe University, Faculty of Science and Literatures, Molecular Biology and Genetic Department, 03200, Afyonkarahisar, Turkey.
authors.forename: Şöhret Yüksek
authors.initials: ŞY
authors.lastname: Kaygısız
authors.affiliation: Uşak University, 1 September Campus Faculty of Arts and Sciences Molecular Biology and Genetic Department, 64200 Uşak, Turkey.
authors.forename: Recep
authors.initials: R
authors.lastname: Liman
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations / Q000187:drug effects; D003035:Cobalt / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage; D054750:Ecotoxicology; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D009835:Oligochaeta / Q000187:drug effects / Q000235:genetics*
chemical_list: D009153:Mutagens; D003035:Cobalt; C018021:cobaltous chloride
keywords: Cobalt chloride; Comet assay; Cytotoxicity; Eisenia hortensis; Genotoxicity; Micronucleus test