title: Evaluation of antioxidative and antitumor activities of extracted flavonoids from Pink Lady apples in human colon and breast cancer cell lines.
abstract: The antioxidative and anticancer effects of extracted flavonoids from Pink Lady apples on human colon cancer LoVo cells and breast cancer MCF-7 cells were evaluated. It was found that the antioxidative property of the peel-flavonoids (Peel-F) was more effective than that of the flesh-flavonoids (Flesh-F). Meanwhile, both the Peel-F and Flesh-F can inhibit cancer cell growth in a dose-dependent manner, with the IC50 values of 110.33 ± 2.52 mg mL(-1) and 378.14 ± 1.64 mg mL(-1) for LoVo cells and 58.42 ± 1.39 mg mL(-1) and 296.06 ± 3.71 mg mL(-1) for MCF-7 cells. This led to the conclusion that the Peel-F were more effective against cancer cells than the Flesh-F, and that the scavenging ROS effects were significantly higher in the Peel-F in vitro. Moreover, we also found that the generation of ROS is a critical mediator in apple flavonoid-induced cell apoptosis and the induction effect of the Peel-F was significantly higher than that of the Flesh-F.
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China. hshzh1965@snnu.edu.cn.
authors.forename: Shufang
authors.initials: S
authors.lastname: Yang
authors.forename: Haisheng
authors.initials: H
authors.lastname: Zhang
authors.forename: Xingbin
authors.initials: X
authors.lastname: Yang
authors.forename: Yilin
authors.initials: Y
authors.lastname: Zhu
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003106:Colon / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells; D027845:Malus / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D059808:Polyphenols; D017382:Reactive Oxygen Species