title: The complete mitochondrial genome of the hybrid of Megalobrama amblycephala (♀) × Megalobrama skolkovii (♂).
abstract: The complete mitochondrial genome of the hybrid of Megalobrama amblycephala (♀) × Megalobrama skolkovii (♂) was characterized first in this study. The total length of the genome was identical to the female parent as 16 623 bp, and the overall base composition was 31.23% A, 24.69% T, 27.89% C, and 16.19% G, with a slight A + T bias. It contained 13 protein-coding genes (PCGs), 22 transfer RNA genes, 2 ribosomal RNA genes, and 2 main non-coding regions (the control region and the origin of the light-strand replication). This study discovered the 99.88% sequence identity between the hybrid and its female parent, which confirmed the maternal inheritance pattern followed by the mitochondrial genome of the hybrid. However, the sequence alignment of mitochondrial genomes between the hybrid and its female parent revealed a total of 20 variable sites in 10 genes or regions, especially 4 sense mutations in 2 PCGs (COX1 and ATPase6). The complete mitochondrial genome sequence of this hybrid bream may provide an important dataset for further study in mitochondrial inheritance mechanism.
authors.affiliation: a College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , Hubei , People's Republic of China and.|b Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan , People's Republic of China.
authors.forename: Ningnan
authors.initials: N
authors.lastname: Guan
authors.affiliation: a College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , Hubei , People's Republic of China and.|b Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan , People's Republic of China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Nie
authors.affiliation: a College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , Hubei , People's Republic of China and.|b Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan , People's Republic of China.
authors.forename: Ruijing
authors.initials: R
authors.lastname: Geng
authors.affiliation: a College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , Hubei , People's Republic of China and.|b Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan , People's Republic of China.
authors.forename: Zexia
authors.initials: Z
authors.lastname: Gao
authors.affiliation: a College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , Hubei , People's Republic of China and.|b Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan , People's Republic of China.
authors.forename: Xiujie
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001482:Base Composition / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D002678:Chimera / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D003530:Cyprinidae / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D023061:Gene Order / Q000235:genetics; D050259:Genes, Mitochondrial / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D008928:Mitochondria / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D004272:DNA, Mitochondrial
keywords: Hybrid of Megalobrama amblycephala (♀) × Megalobrama skolkovii (♂); maternal inheritance; mitochondrial genome; sense mutation