title: Leverage principle of retardation signal in titration of double protein via chip moving reaction boundary electrophoresis.
abstract: In the present work we address a simple, rapid and quantitative analytical method for detection of different proteins present in biological samples. For this, we proposed the model of titration of double protein (TDP) and its relevant leverage theory relied on the retardation signal of chip moving reaction boundary electrophoresis (MRBE). The leverage principle showed that the product of the first protein content and its absolute retardation signal is equal to that of the second protein content and its absolute one. To manifest the model, we achieved theoretical self-evidence for the demonstration of the leverage principle at first. Then relevant experiments were conducted on the TDP-MRBE chip. The results revealed that (i) there was a leverage principle of retardation signal within the TDP of two pure proteins, and (ii) a lever also existed within these two complex protein samples, evidently demonstrating the validity of TDP model and leverage theory in MRBE chip. It was also showed that the proposed technique could provide a rapid and simple quantitative analysis of two protein samples in a mixture. Finally, we successfully applied the developed technique for the quantification of soymilk in adulterated infant formula. The TDP-MRBE opens up a new window for the detection of adulteration ratio of the poor food (milk) in blended high quality one.
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Liu-Xia
authors.initials: LX
authors.lastname: Zhang
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Yi-Ren
authors.initials: YR
authors.lastname: Cao
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Biochemistry, Oklahoma University, Norman, OK 73019, USA.
authors.forename: Shao-Rong
authors.initials: SR
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Meng
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; Department of Chemistry and Biochemistry, Oklahoma University, Norman, OK 73019, USA. Electronic address: lyfan@sjtu.edu.cn.
authors.forename: Liu-Yin
authors.initials: LY
authors.lastname: Fan
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: cxcao@sjtu.edu.cn.
authors.forename: Cheng-Xi
authors.initials: CX
authors.lastname: Cao
mesh_terms: D000465:Algorithms; D000818:Animals; D045424:Complex Mixtures / Q000032:analysis; D003198:Computer Simulation; D004586:Electrophoresis / Q000295:instrumentation*; D005504:Food Analysis / Q000295:instrumentation*; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis*; D008956:Models, Chemical
chemical_list: D045424:Complex Mixtures; D008894:Milk Proteins
keywords: Chip electrophoresis; Leverage principle; Moving reaction boundary; Protein titration; Retardation signal