title: A lipid transfer protein, OsLTPL36, is essential for seed development and seed quality in rice.
abstract: Storage lipid is a vital component for maintaining structure of seed storage substances and valuable for rice quality and food texture. However, the knowledge of lipid transporting related genes and their function in seed development have not been well elucidated yet. In this study, we identified OsLTPL36, a homolog of putative lipid transport protein, and showed specific expression in rice developing seed. Transcriptional profiling and in situ hybridization analysis confirmed that OsLTPL36 was exclusively expressed in developing seed coat and endosperm aleurone cells. Down-regulated expression of OsLTPL36 led to decreased seed setting rate and 1000-grain weight in transgenic plants. Further studies showed that suppressed expression of OsLTPL36 caused chalky endosperm and resulted in reduced fat acid content in RNAi lines as compared with wild type (WT). Histological analysis showed that the embryo development was delayed after down regulation of OsLTPL36. Moreover, impeded seed germination and puny seedling were also observed in the OsLTPL36 RNAi lines. The data demonstrated that OsLTPL36, a lipid transporter, was critical important not only for seed quality but also for seed development and germination in rice.
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: wx3177@webmail.hzau.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: wszw523@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: loovy@webmail.hzau.edu.cn.
authors.forename: Zhanhua
authors.initials: Z
authors.lastname: Lu
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: diana1983941@mail.hzau.edu.cn.
authors.forename: Yidan
authors.initials: Y
authors.lastname: Ouyang
authors.affiliation: Life science Faculty, Kim Il Sung University, Pyongyang 999093, Democratic People's Republic of Korea. Electronic address: ocs75212@163.com.
authors.forename: Chol Su
authors.initials: CS
authors.lastname: O
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: yaojlmy@mail.hzau.edu.cn.
authors.forename: Jialing
authors.initials: J
authors.lastname: Yao
mesh_terms: D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018525:Germination; D012275:Oryza / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D010940:Plant Proteins; C030363:lipid transfer protein
keywords: Fat acid content; Floury endosperm; Lipid transfer protein; Seed development; Seed germination