title: Vicia faba bioassay for environmental toxicity monitoring: A review.
abstract: Higher plants are recognized as excellent genetic models to detect cytogenetic and mutagenic agents and are frequently used in environmental monitoring studies. Vicia faba (V. faba) bioassay have been used to study DNA damages i.e., chromosomal and nuclear aberrations induced by metallic compounds, pesticides, complex mixtures, petroleum derivates, toxins, nanoparticles and industrial effluents. The main advantages of using V. faba is its availability round the year, economical to use, easy to grow and handle; its use does not require sterile conditions, rate of cell division is fast, chromosomes are easy to score, less expensive and more sensitive as compared to other short-term tests that require pre-preparations. The V. faba test offers evaluation of different endpoints and tested agents can be classified as cytotoxic/genotoxic/mutagenic. This test also provides understanding about mechanism of action, whether the tested agent is clastogenic or aneugenic in nature. In view of advantages offered by V. faba test system, it is used extensively to assess toxic agents and has been emerged as an important bioassay for ecotoxicological studies. Based on the applications of V. faba test to assess the environmental quality, this article offers an overview of this test system and its efficiency in assessing the cytogenetic and mutagenic agents in different classes of the environmental concerns.
authors.affiliation: National Centre of Excellence in Physical Chemistry, University of Peshawar, Peshawar 25120, Pakistan. Electronic address: bosalvee@yahoo.com.
authors.forename: Munawar
authors.initials: M
authors.lastname: Iqbal
mesh_terms: D001681:Biological Assay / Q000379:methods*; D054750:Ecotoxicology; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000633:toxicity*; D006801:Humans; D031307:Vicia faba / Q000166:cytology / Q000187:drug effects* / Q000235:genetics
chemical_list: D004785:Environmental Pollutants
keywords: Bioassay; Broad bean; Contamination; Cytotoxicity; Ecotoxicology; Genotoxicity; Mutagenicity; Vicia faba; Wastewater