title: Proteomic and bioinformatics profile of paired human alveolar macrophages and peripheral blood monocytes.
abstract: Little is known about proteomic differences between pluripotent human peripheral blood monocytes (MN) and their terminally-differentiated pulmonary counterparts, alveolar macrophages (AM). To better characterize these cell populations, we performed a label-free shotgun proteomics assessment of matched AM and MN preparations from eight healthy volunteers. With an FDR of less than 0.45%, we identified 1754 proteins within AM and 1445 from MN. Comparison of the two proteomes revealed that 1239 of the proteins found in AM were shared with MN, whereas 206 proteins were uniquely identified in MN and 515 were unique to AM. Molecular and cellular functions, protein classes, development associations, and membership in physiological systems and canonical pathways were identified among the detected proteins. Analysis of biologic processes represented by these proteomes indicated that MN were most prominently enriched for proteins involved in cellular movement and immune cell trafficking. In contrast, AM were enriched for proteins involved in protein trafficking, molecular transport, and cellular assembly and organization. These findings provide a baseline proteomic resource for further studies aimed at better understanding of the functional differences between MN and AM in both health and disease.
authors.affiliation: Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, OH, USA.|Division of Pulmonary, Critical Care, and Sleep Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, USA.
authors.forename: Sara E
authors.initials: SE
authors.lastname: Tomechko
authors.affiliation: Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, OH, USA.
authors.forename: Kathleen C
authors.initials: KC
authors.lastname: Lundberg
authors.affiliation: Division of Pulmonary, Critical Care, and Sleep Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, USA.|The Louis Stokes Cleveland Department of Veterans' Affairs Medical Center, Cleveland, OH, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Jarvela
authors.affiliation: Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, OH, USA.
authors.forename: Gurkan
authors.initials: G
authors.lastname: Bebek
authors.affiliation: Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, OH, USA.
authors.forename: Nicole G
authors.initials: NG
authors.lastname: Chesnokov
authors.affiliation: Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, OH, USA.
authors.forename: Daniela
authors.initials: D
authors.lastname: Schlatzer
authors.affiliation: Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, OH, USA.
authors.forename: Rob M
authors.initials: RM
authors.lastname: Ewing
authors.affiliation: University Hospitals Case Medical Center, Cleveland, OH, USA.|Division of Infectious Diseases and HIV Medicine, Case Western Reserve University, Cleveland, OH, USA.
authors.forename: W Henry
authors.initials: WH
authors.lastname: Boom
authors.affiliation: Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, OH, USA.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Chance
authors.affiliation: Division of Pulmonary, Critical Care, and Sleep Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, USA.|The Louis Stokes Cleveland Department of Veterans' Affairs Medical Center, Cleveland, OH, USA.|University Hospitals Case Medical Center, Cleveland, OH, USA.
authors.forename: Richard F
authors.initials: RF
authors.lastname: Silver
mesh_terms: D000328:Adult; D019295:Computational Biology; D006801:Humans; D016676:Macrophages, Alveolar / Q000737:chemistry*; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D015398:Signal Transduction; D055815:Young Adult
chemical_list: D020543:Proteome
keywords: Alveolar macrophage; Cell biology; Human immune response; Lung; Peripheral blood monocytes; Shotgun proteomics