title: Gal-1 silenced trophoblast tumor cells (BeWo) show decreased syncytium formation and different miRNA production compared to non-target silenced BeWo cells.
abstract: Galectin-1 (gal-1), a member of the mammalian β-galactoside-binding proteins, exerts biological effects by recognition of glycan ligands, including those involved in cell adhesion and growth regulation. In previous studies, we demonstrated that gal-1 induces cell differentiation processes on the membrane of choriocarcinoma cells BeWo, including the receptor tyrosine kinases (RTKs) REarranged during Transfection (RET), Janus Kinase 2 (JAK2) and Vascular endothelial growth factor receptor 3 (VEGFR3). Furthermore, Mitogen-Activated Protein Kinases (MAPK) and serine/threonine kinases were phosphorylated by gal-1. In addition, gal-1 in trophoblast cells in vitro induced syncytium formation especially after concentration dependent stimulation of the cells with this galectin. This is in contrast to MAPK-inhibitor U0126 that reduced syncytium formation of BeWo cells. The aim of this study was to analyze the syncytium formation abilities of BeWo cells that were gal-1 silenced. We found a significantly reduced syncytium formation rate in gal-1 silenced BeWo cells. In addition, these cells show a different miRNA expression profile. In summary, we found that gal-1 is a major trigger for fusion processes in BeWo cells. This function is accompanied by different regulation of miRNA synthesis in the BeWo cell culture model.
authors.affiliation: a Ludwig Maximilians University of Munich , Department of Obstetrics and Gynecology , Munich , Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Hutter
authors.affiliation: b University Hospital Jena , Department of Obstetrics , Placenta Lab , Jena , Germany.
authors.forename: Diana M
authors.initials: DM
authors.lastname: Morales-Prieto
authors.affiliation: a Ludwig Maximilians University of Munich , Department of Obstetrics and Gynecology , Munich , Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Andergassen
authors.affiliation: a Ludwig Maximilians University of Munich , Department of Obstetrics and Gynecology , Munich , Germany.
authors.forename: Lisa
authors.initials: L
authors.lastname: Tschakert
authors.affiliation: a Ludwig Maximilians University of Munich , Department of Obstetrics and Gynecology , Munich , Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Kuhn
authors.affiliation: a Ludwig Maximilians University of Munich , Department of Obstetrics and Gynecology , Munich , Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Hofmann
authors.affiliation: b University Hospital Jena , Department of Obstetrics , Placenta Lab , Jena , Germany.
authors.forename: Udo R
authors.initials: UR
authors.lastname: Markert
authors.affiliation: a Ludwig Maximilians University of Munich , Department of Obstetrics and Gynecology , Munich , Germany.
authors.forename: Udo
authors.initials: U
authors.lastname: Jeschke
mesh_terms: D015820:Cadherins / Q000378:metabolism; D002459:Cell Fusion; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D037483:Galectin 1 / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D015726:Giant Cells / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012995:Solubility; D013194:Staining and Labeling; D014328:Trophoblastic Neoplasms / Q000235:genetics* / Q000473:pathology*; D014327:Trophoblasts; D051176:beta Catenin / Q000378:metabolism
chemical_list: D015820:Cadherins; D037483:Galectin 1; D035683:MicroRNAs; D012333:RNA, Messenger; D051176:beta Catenin
keywords: BeWo; galectin-1; miRNA; syncytium formation