title: Description of Diutina gen. nov., Diutina siamensis, f.a. sp. nov., and reassignment of Candida catenulata, Candida mesorugosa, Candida neorugosa, Candida pseudorugosa, Candida ranongensis, Candida rugosa and Candida scorzettiae to the genus Diutina.
abstract: Three strains (DMKU-RE28, DMKU-RE43T and DMKU-RE123) of a novel anamorphic yeast species were isolated from rice leaf tissue collected in Thailand. DNA sequence analysis demonstrated that the species forms a sister pair with Candida ranongensis CBS 10861T but differs by 24-30 substitutions in the LSU rRNA gene D1/D2 domains and 30-35 substitutions in the ITS region. A phylogenetic analysis based on both the small and the large rRNA gene subunits confirmed this connection and demonstrated the presence of a clade that also includes Candida catenulata, Candida mesorugosa, Candida neorugosa, Candida pseudorugosa, Candida rugosa and Candida scorzettiae. The clade is not closely affiliated to any known teleomorphic genus, and forms a well-separated lineage from currently recognized genera of the Saccharomycetales. Hence, the genus Diutina gen. nov. is proposed to accommodate members of the clade, including Diutina siamensis f.a. sp. nov. and the preceding seven Candida species. The type strain is DMKU-RE43T ( = CBS 13388T = BCC 61183T = NBRC 109695T).
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Pannida
authors.initials: P
authors.lastname: Khunnamwong
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Noppon
authors.initials: N
authors.lastname: Lertwattanasakul
authors.affiliation: Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani, Thailand.
authors.forename: Sasitorn
authors.initials: S
authors.lastname: Jindamorakot
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.|Center for Advanced Studies in Tropical Natural Resources, National Research University Kasetsart University, Thailand.
authors.forename: Savitree
authors.initials: S
authors.lastname: Limtong
authors.affiliation: Department of Biology, University of Western Ontario, London, Ontario, Canada.
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Lachance
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology*; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D013785:Thailand
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal