title: The Prevalence of IS Aba 1 and IS Aba 4 in Acinetobacter baumannii Species of Different International Clone Lineages Among Patients With Burning in Tehran, Iran.
abstract: BACKGROUND
  Multidrug resistant strains of Acinetobacter baumannii (MDR-AB) have emerged as alarming nosocomial pathogens among patients with burning.
  
  
  OBJECTIVE
  The current study aimed to determine the susceptibility of A. baumannii species, carbapenems resistance patterns, and their association with IS Aba 1 and IS Aba 4 elements upstream of the bla OXA-like genes, and the distribution of international clone (IC) of A. baumannii isolates among patients with burning in Tehran, Iran.
  
  
  METHODS
  In the current study, 62 A. baumannii species isolates from patients with burning in Tehran, Iran, in 2012 were evaluated for the antimicrobial susceptibility, genetic relationships, ICs, carbapenemase encoding genes, and insertion elements IS Aba upstream of bla OXA-like genes.
  
  
  RESULTS
  The highest rates of susceptibility were observed with colistin (88.7%) and tigecycline (82.2%). The extensively drug-resistance and pan drug-resistance were observed in 37.1% and 8.1% of the isolates, respectively. About 98.3% of 17 genotypes categorized into three distinct clusters. Thirty-six of the 62 isolates (58%) belonged to the IC II lineage. The most prevalent acquired OXA-type carbapenemase was bla OXA-23-like (62.9%). IS Aba 1 and IS Aba 4 were detected upstream of bla OXA-23-like genes in 45.1% and 12.9% of isolates, respectively. In 32.2% of all isolates, IS Aba 1 laid upstream of bla OXA-51-like genes. The PCR results were negative for carbapenemase genes of Ambler class A and B, except bla VIM-2 . (1.6%).
  
  
  CONCLUSIONS
  It was the first study that attempted to detect the insertion elements IS Aba and IC lineages in MDR-AB species isolated from patients with burning in Iran.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Bahador
authors.affiliation: Department of Genetic, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Raoo An
authors.affiliation: Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Farshadzadeh
authors.affiliation: Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Leyli
authors.initials: L
authors.lastname: Beitollahi
authors.affiliation: Department of Microbiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Azad
authors.initials: A
authors.lastname: Khaledi
authors.affiliation: Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Rahimi
authors.affiliation: Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Mokhtaran
authors.affiliation: Health Management Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mehrabi Tavana
authors.affiliation: Applied Microbiology Research Center, Department of Microbiology, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Esmaeili
keywords: Acinetobacter baumannii; Carbapenemase; Drug Resistance; IS elements; Molecular Epidemiology; beta-Lactamases