title: Comprehensive analysis of microRNA expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor.
abstract: MicroRNAs (miRNAs) have been implicated in regulating multiple processes during brain development in various species. However, the function of miRNAs in human brain development remains largely unexplored. Here, we provide a comprehensive analysis of miRNA expression of regionalized neural progenitor cells derived from human embryonic stem cells and human foetal brain. We found miR-92b-3p and miR-130b-5p to be specifically associated with neural progenitors and several miRNAs that display both age-specific and region-specific expression patterns. Among these miRNAs, we identified miR-10 to be specifically expressed in the human hindbrain and spinal cord, while being absent from rostral regions. We found that miR-10 regulates a large number of genes enriched for functions including transcription, actin cytoskeleton and ephrin receptor signalling. When overexpressed, miR-10 influences caudalization of human neural progenitor cells. Together, these data confirm a role for miRNAs in establishing different human neural progenitor populations. This dataset also provides a comprehensive resource for future studies investigating the functional role of different miRNAs in human brain development.
authors.affiliation: Lab of Molecular Neurogenetics, Wallenberg Neuroscience Center and Lund Stem Cell Center, Lund University, Lund 221 84, Sweden Developmental and Regenerative Neurobiology, Department of Experimental Medical Science, Wallenberg Neuroscience Center and Lund Stem Cell Center, Lund University, Lund 221 84, Sweden.
authors.forename: Marie E
authors.initials: ME
authors.lastname: Jönsson
authors.affiliation: Developmental and Regenerative Neurobiology, Department of Experimental Medical Science, Wallenberg Neuroscience Center and Lund Stem Cell Center, Lund University, Lund 221 84, Sweden.
authors.forename: Jenny
authors.initials: J
authors.lastname: Nelander Wahlestedt
authors.affiliation: Lab of Molecular Neurogenetics, Wallenberg Neuroscience Center and Lund Stem Cell Center, Lund University, Lund 221 84, Sweden.
authors.forename: Malin
authors.initials: M
authors.lastname: Åkerblom
authors.affiliation: Developmental and Regenerative Neurobiology, Department of Experimental Medical Science, Wallenberg Neuroscience Center and Lund Stem Cell Center, Lund University, Lund 221 84, Sweden.
authors.forename: Agnete
authors.initials: A
authors.lastname: Kirkeby
authors.affiliation: Lab of Molecular Neurogenetics, Wallenberg Neuroscience Center and Lund Stem Cell Center, Lund University, Lund 221 84, Sweden.
authors.forename: Josephine
authors.initials: J
authors.lastname: Malmevik
authors.affiliation: Lab of Molecular Neurogenetics, Wallenberg Neuroscience Center and Lund Stem Cell Center, Lund University, Lund 221 84, Sweden.
authors.forename: Per Ludvik
authors.initials: PL
authors.lastname: Brattaas
authors.affiliation: Lab of Molecular Neurogenetics, Wallenberg Neuroscience Center and Lund Stem Cell Center, Lund University, Lund 221 84, Sweden johan.jakobsson@med.lu.se malin.parmar@med.lu.se.
authors.forename: Johan
authors.initials: J
authors.lastname: Jakobsson
authors.affiliation: Developmental and Regenerative Neurobiology, Department of Experimental Medical Science, Wallenberg Neuroscience Center and Lund Stem Cell Center, Lund University, Lund 221 84, Sweden johan.jakobsson@med.lu.se malin.parmar@med.lu.se.
authors.forename: Malin
authors.initials: M
authors.lastname: Parmar
mesh_terms: D000704:Analysis of Variance; D001921:Brain / Q000196:embryology* / Q000254:growth & development*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D022202:Chromosomes, Artificial, Bacterial; D017931:DNA Primers / Q000235:genetics; D005434:Flow Cytometry; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins; D006801:Humans; D016086:Lentivirus; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D058953:Neural Stem Cells / Q000378:metabolism* / Q000502:physiology; D016133:Polymerase Chain Reaction; D060888:Real-Time Polymerase Chain Reaction; D015183:Restriction Mapping; D055748:SOXB1 Transcription Factors / Q000235:genetics
chemical_list: D017931:DNA Primers; C547183:MIRN10 microRNA, human; D035683:MicroRNAs; C490906:SOX1 protein, human; D055748:SOXB1 Transcription Factors; D049452:Green Fluorescent Proteins
keywords: Cell specification; MicroRNA; Neural development; Neural stem cells