title: Terrein reduces age-related inflammation induced by oxidative stress through Nrf2/ERK1/2/HO-1 signalling in aged HDF cells.
abstract: This study investigated whether multiple bioactivity of terrein such as anti-inflammatory and anti-oxidant inhibits age-related inflammation by promoting an antioxidant response in aged human diploid fibroblast (HDF) cells. HDF cells were cultured serially for in vitro replicative senescence. To create the ageing cell phenotype, intermediate stage (PD31) HDF cells were brought to stress-induced premature senescence (SIPS) using hydrogen peroxide (H2 O2). Terrein increased cell viability even with H2O2 stress and reduced inflammatory molecules such as intracellular adhesion molecule-1 (ICAM-1), cyclooxygenase-2 (COX-2), interleukin-1beta (IL-1β) and tumour necrosis factor-alpha (TNF-α). Terrein reduced also phospho-extracellular kinase receptor1/2 (p-EKR1/2) signalling in aged HDF cells. SIPS cells were attenuated for age-related biological markers including reactive oxygen species (ROS), senescence associated beta-galactosidase (SA β-gal.) and the aforementioned inflammatory molecules. Terrein induced the induction of anti-oxidant molecules, copper/zinc-superoxide defence (Cu/ZnSOD), manganese superoxide dismutase (MnSOD) and heme oxygenase-1 (HO-1) in SIPS cells. Terrein also alleviated reactive oxygen species formation through the Nrf2/HO-1/p-ERK1/2 pathway in aged cells. The results indicate that terrein has an alleviative function of age-related inflammation characterized as an anti-oxidant. Terrein might be a useful nutraceutical compound for anti-ageing.
authors.affiliation: Departments of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Korea.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of New Drug Discovery and Development, Chungnam National University, Daejeon, Korea.
authors.forename: Sook-Jeong
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Departments of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Jung
authors.affiliation: Departments of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Korea.
authors.forename: Jeong-Seok
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Departments of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Korea.
authors.forename: Nan-Hee
authors.initials: NH
authors.lastname: Lee
authors.affiliation: Departments of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Korea.
authors.forename: Ho-Keun
authors.initials: HK
authors.lastname: Yi
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000276:immunology; D000975:Antioxidants / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D020935:MAP Kinase Signaling System / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D000975:Antioxidants; D003517:Cyclopentanes; C439087:terrein
keywords: Nrf2; SIPS; ageing; antioxidant; oxidative stress; terrein