title: Cell adhesion molecule contactin-associated protein 3 is expressed in the mouse basal ganglia during early postnatal stages.
abstract: Cell adhesion molecules play important roles in the development of the nervous system. Among the contactin-associated protein (Caspr; also known as Cntnap) family, which belongs to the neurexin superfamily of proteins, Caspr and Caspr2 are indispensable for the formation and maintenance of myelinated nerves. In contrast, a physiological role for Caspr3 remains to be elucidated. This study examines the expression and localization of Caspr3 in the mouse brain using newly generated Caspr3 antibodies. Caspr3 was expressed abundantly between the first and the second postnatal weeks. During this period, Caspr3 was localized especially to the basal ganglia, including the striatum, external segment of the globus pallidus, and substantia nigra, and no gross abnormalities were apparent in the basal ganglia of Caspr3 knockout mice. In the striatum, Caspr3 was expressed by a subpopulation of medium spiny neurons that constitute the direct and indirect pathways. Caspr3 immunostaining was observed as punctate around the cell bodies as well as in the soma. These Caspr3 signals did not, however, overlap with those of synaptic markers. Our findings suggest that Caspr3 may play an important role in basal ganglia development during early postnatal stages.
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan.
authors.forename: Haruna
authors.initials: H
authors.lastname: Hirata
authors.affiliation: Mouse Genomics Resource Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
authors.forename: Juzoh
authors.initials: J
authors.lastname: Umemori
authors.affiliation: Mouse Genomics Resource Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yoshioka
authors.affiliation: Mouse Genomics Resource Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Koide
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan.|Nagaoka National College of Technology, Nagaoka, Niigata, Japan.
authors.forename: Kazutada
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan.
authors.forename: Yasushi
authors.identifier: http://orcid.org/0000-0001-9230-6784
authors.initials: Y
authors.lastname: Shimoda
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001479:Basal Ganglia / Q000254:growth & development* / Q000378:metabolism*; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32 / Q000378:metabolism; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014404:Tubulin / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: C000609354:Caspr3 protein, mouse; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D014404:Tubulin; D014446:Tyrosine 3-Monooxygenase
keywords: external segment of the globus pallidus; medium spiny neuron; striatum; strionigral fiber; substantia nigra