title: A Novel Reduplicate Strategy for Tracing Hemostatic Compounds from Heating Products of the Flavonoid Extract in Platycladi cacumen by Spectrum-Effect Relationships and Column Chromatography.
abstract: Platycladi cacumen and its processed product have been utilized as a Chinese medicine to treat hemorrhages. In this study, the base peak chromatogram fingerprints of heating products of total flavonoids in Platycladi cacumen were established by high performance liquid chromatography coupled with mass spectroscopy/mass spectroscopy (HPLC-MS/MS), and the hemostatic activities were studied by hemostatic screening tests in vivo. The spectrum-effect relationships between fingerprints and hemostatic activities were analyzed by using canonical correlation analysis to trace the peaks responsible for the significant hemostatic effects. Peak 10 and peak 12 were correlated most closely, thus probably being the main hemostatic compounds. To confirm the reliability of this strategy, the targeted unknown peak was obtained by bioactivity-guided isolation, characterized by MS, ¹H-NMR, (13)C-NMR, and 2D-NMR spectroscopies, and referred to as cecarbon as a new compound. In addition, the isolated compound exhibited hemostatic effect in a dose-dependent manner with different potencies in vitro and existed in Platycladi cacumen Carbonisatus. A novel dereplication strategy was employed to trace and identify the active compounds of other herbs that have bioactivity enhancement after processing using spectrum-effect relationships and column chromatography.
authors.affiliation: School of pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. cyqzs88@163.com.
authors.forename: Yeqing
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. yuhongli76@126.com.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China. yuhongli76@126.com.|Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China. yuhongli76@126.com.
authors.forename: Hongli
authors.initials: H
authors.lastname: Yu
authors.affiliation: School of pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. whao5795@163.com.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China. whao5795@163.com.|Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China. whao5795@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: School of pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. pan_yaozong@126.com.
authors.forename: Yaozong
authors.initials: Y
authors.lastname: Pan
authors.affiliation: School of pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. wjwkl@126.com.
authors.forename: Kuilong
authors.initials: K
authors.lastname: Wang
authors.affiliation: School of pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. liuliping1487@163.com.
authors.forename: Liping
authors.initials: L
authors.lastname: Liu
authors.affiliation: School of pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. yangpingctt@126.com.
authors.forename: Yangping
authors.initials: Y
authors.lastname: Jin
authors.affiliation: School of pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. didiaodexiaojuren@163.com.
authors.forename: Chengchao
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D029779:Cupressaceae / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D005260:Female; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006490:Hemostatics / Q000737:chemistry* / Q000494:pharmacology; D006358:Hot Temperature; D008297:Male; D015394:Molecular Structure; D051381:Rats; D053719:Tandem Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006490:Hemostatics
keywords: Platycladi cacumen; flavonoids; heating products; hemostatic activity; spectrum-effect relationships