title: In vitro evaluation of the wound healing activity of Drypetes klainei stem bark extracts.
abstract: BACKGROUND
  Drypetes klainei Pierre ex Pax is used in Cameroon by Baka Pygmies in the wound healing process and for the treatment of burns.
  
  
  OBJECTIVE
  To validate the traditional use of D. klainei Pierre ex Pax stem bark extracts through the evaluation of their antimicrobial properties and their ability to improve wound healing process in fibroblast cell cultures.
  
  
  METHODS
  The antimicrobial properties of D. klainei extracts were evaluated against Staphylococcus aureus ATCC 6538, Streptococcus pyogenes ATCC 19615, Escherichia coli ATCC 10536, Candida albicans ATCC 10231, on the basis of the minimum inhibitory concentration (MIC) and the minimum bactericidal-fungicidal concentration (MBC-MFC) by the macrodilution method. The extracts abilities to accelerate wound healing were studied on murine and human fibroblasts in terms of cell viability and migration (scratch wound-healing assay).
  
  
  RESULTS
  All the extracts were non-toxic against the selected microorganisms at the tested concentrations, and significantly improve wound healing process in vitro, compared to untreated controls. However, the defatted methanol extract was active at lower concentrations, compared to the water extract.
  
  
  CONCLUSIONS
  The ability of both water and defatted methanol extracts to accelerate scratch wound closure in fibroblast cultures may support the traditional use of D. klainei stem bark in the treatment of skin lesions (such as burns) even if no antimicrobial activity was evidenced.
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy. Electronic address: gloria.brusotti@unipv.it.
authors.forename: G
authors.initials: G
authors.lastname: Brusotti
authors.affiliation: Institute of Translational Pharmacology, National Research Council of Italy, Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Andreola
authors.affiliation: Institute of Translational Pharmacology, National Research Council of Italy, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Sferrazza
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Grisoli
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Merelli
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: F S
authors.initials: FS
authors.lastname: Robustelli della Cuna
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Calleri
authors.affiliation: Institute of Translational Pharmacology, National Research Council of Italy, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Nicotera
authors.affiliation: Institute of Translational Pharmacology, National Research Council of Italy, Rome, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Pierimarchi
authors.affiliation: Institute of Translational Pharmacology, National Research Council of Italy, Rome, Italy. Electronic address: annalucia.serafino@ift.cnr.it.
authors.forename: A
authors.initials: A
authors.lastname: Serafino
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D002460:Cell Line; D002478:Cells, Cultured; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D019684:Magnoliopsida; D051379:Mice; D008826:Microbial Sensitivity Tests; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013297:Streptococcus pyogenes / Q000187:drug effects / Q000254:growth & development; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D010936:Plant Extracts
keywords: Bark extract; Cell proliferation; Drypetes klainei Pierre ex Pax; Wound healing