title: Transcriptome screening and verification of genes related to metabolism affected by histone deacetylase inhibitors.
abstract: Histone deacetylases (HDACs) are responsible for catalyzing the deacetylation of histones, which closely related to many biological processes such as cell proliferation, differentiation and apoptosis. In recent years, HDAC inhibitors (HADCIs), with the anti-tumor potential, have been hot-spots of drug screening. Although the latest studies suggested that HDAC2 might influence the metabolism, the mechanism of HDACIs in metabolic regulation is still unclear. Here, we integrated the gene expression profiling of HDACIs (TSA and SAHA) in hepatocellular carcinoma cell (HepG2). The results showed 380 differentially expressed genes (DEGs) and 35 KEGG pathways enriched by DEGs in TSA-treatment group. Most of DEGs (177/380) and KEGG pathways (23/35) from TSA-treatment groups were confirmed by SAHA-treatment. About half of KEGG pathways (9/23) were related to metabolism ,and nearly one third of common DEGs (66/177) were involved in metabolic process. Moreover, HDAC2 siRNA experiment verified the effect of HDACIs on metabolic genes, suggesting that HDACIs potentially present a practical value to prevent tumor and other metabolism-related diseases.
authors.affiliation: Jiujiang Third People's Hospital, Jiujiang 332000, China.
authors.forename: Ji-hong
authors.initials: JH
authors.lastname: Cao
authors.affiliation: Institute for Translational Medicine, Nanchang University, Nanchang 330000, China.
authors.forename: Wei-ting
authors.initials: WT
authors.lastname: Liao
authors.affiliation: Jiujiang Third People's Hospital, Jiujiang 332000, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wo
authors.affiliation: Jiujiang Third People's Hospital, Jiujiang 332000, China.
authors.forename: Guo-rong
authors.initials: GR
authors.lastname: Xu
authors.affiliation: Jiujiang Third People's Hospital, Jiujiang 332000, China.
authors.forename: Huan-xin
authors.initials: HX
authors.lastname: Xu
authors.affiliation: Jiujiang Third People's Hospital, Jiujiang 332000, China.
authors.forename: Ping-long
authors.initials: P
authors.lastname: Li
authors.affiliation: Jiujiang Third People's Hospital, Jiujiang 332000, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Jiujiang Third People's Hospital, Jiujiang 332000, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Institute for Translational Medicine, Nanchang University, Nanchang 330000, China.
authors.forename: Jia-ri
authors.initials: JR
authors.lastname: Lin
authors.affiliation: The Second Affiliated Hospital, Nanchang University, Nanchang 330000, China.
authors.forename: Lian-rui
authors.initials: LR
authors.lastname: Deng
mesh_terms: D015870:Gene Expression / Q000187:drug effects; D056945:Hep G2 Cells; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D059467:Transcriptome; D000077337:Vorinostat
chemical_list: D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C012589:trichostatin A; D000077337:Vorinostat