title: Characteristics of Cyclin B and its potential role in regulating oogenesis in the red claw crayfish (Cherax quadricarinatus).
abstract: Cyclin B is a regulatory subunit of maturation-promoting factor (MPF), which has a key role in the induction of meiotic maturation of oocytes. MPF has been studied in a wide variety of animal species; however, its expression in crustaceans is poorly characterized. In this study, the complete cDNA sequence of Cyclin B was cloned from the red claw crayfish, Cherax quadricarinatus, and its spatiotemporal expression profiles were analyzed. Cyclin B cDNA (1779 bp) encoded a 401 amino acid protein with a calculated molecular weight of 45.1 kDa. Quantitative real-time PCR demonstrated that Cyclin B mRNA was expressed mainly in the ovarian tissue and that the expression decreased as the ovaries developed. Immunofluorescence analysis revealed that the Cyclin B protein relocated from the cytoplasm to the nucleus during oogenesis. These findings suggest that Cyclin B plays an important role in gametogenesis and gonad development in C. quadricarinatus.
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Wuxi, China.
authors.forename: L M
authors.initials: LM
authors.lastname: Wang
authors.affiliation: Life Science College, East China Normal University, Shanghai, China.
authors.forename: W W
authors.initials: WW
authors.lastname: Lv
authors.affiliation: Life Science College, East China Normal University, Shanghai, China.
authors.forename: D
authors.initials: D
authors.lastname: Zuo
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Wuxi, China.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Dong
authors.affiliation: Life Science College, East China Normal University, Shanghai, China ylzhao@bio.ecnu.edu.cn.
authors.forename: Y L
authors.initials: YL
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D019926:Cyclin B / Q000235:genetics* / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D016200:Maturation-Promoting Factor / Q000235:genetics* / Q000378:metabolism; D008540:Meiosis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009865:Oocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D009866:Oogenesis / Q000235:genetics*; D016366:Open Reading Frames; D010053:Ovary / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D021381:Protein Transport; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D019926:Cyclin B; D018076:DNA, Complementary; D011994:Recombinant Proteins; D016200:Maturation-Promoting Factor