title: Combination of Estrogen and Immunosuppressive Agents to Establish a Mouse Model of Candidiasis with Concurrent Oral and Vaginal Mucosal Infection.
abstract: Mouse model is an appropriate tool for pathogenic determination and study of host defenses during the fungal infection. Here, we established a mouse model of candidiasis with concurrent oral and vaginal mucosal infection. Two C. albicans strains sourced from clinical candidemia (SC5314) and mucosal infection (ATCC62342) were tested in ICR mice. The different combinational panels covering estrogen and immunosuppressive agents, cortisone, prednisolone and cyclophosphamide were used for concurrent oral and vaginal candidiasis establishment. Prednisolone in combination with estrogen proved an optimal mode for concurrent mucosal infection establishment. The model maintained for 1 week with fungal burden reached at least 10(5) cfu/g of tissue. This mouse model was evaluated by in vivo pharmacodynamics of fluconazole and host mucosal immunity of IL-17 and IL-23. Mice infected by SC5314 were cured by fluconazole. An increase in IL-23 in both oral and vaginal homogenates was observed after infection, while IL-17 only had a prominent elevation in oral tissue. This model could properly mimic complicated clinical conditions and provides a valuable means for antifungal assay in vivo and may also provide a useful method for the evaluation of host-fungal interactions.
authors.affiliation: Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Science (CAMS), Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, No. 12. Jiang Wangmiao St., Nanjing, 210042, People's Republic of China.
authors.forename: Le
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Science (CAMS), Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, No. 12. Jiang Wangmiao St., Nanjing, 210042, People's Republic of China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Science (CAMS), Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, No. 12. Jiang Wangmiao St., Nanjing, 210042, People's Republic of China.
authors.forename: Huan
authors.initials: H
authors.lastname: Mei
authors.affiliation: Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Science (CAMS), Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, No. 12. Jiang Wangmiao St., Nanjing, 210042, People's Republic of China.
authors.forename: Yongnian
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Science (CAMS), Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, No. 12. Jiang Wangmiao St., Nanjing, 210042, People's Republic of China.
authors.forename: Guixia
authors.initials: G
authors.lastname: Lv
authors.affiliation: Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Science (CAMS), Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, No. 12. Jiang Wangmiao St., Nanjing, 210042, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zeng
authors.affiliation: Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Science (CAMS), Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, No. 12. Jiang Wangmiao St., Nanjing, 210042, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhan
authors.affiliation: Department of Microbiology and Immunology, Georgetown University Medical Center, SE 305 Med-Dent Building, 3900 Reservoir Rd., NW., Washington, DC, 20057, USA. dl33@georgetown.edu.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Science (CAMS), Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, No. 12. Jiang Wangmiao St., Nanjing, 210042, People's Republic of China. liuweida@medmail.com.cn.
authors.forename: Weida
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage / Q000493:pharmacokinetics; D002175:Candida / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000382:microbiology / Q000473:pathology*; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology / Q000473:pathology*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004967:Estrogens / Q000008:administration & dosage*; D005260:Female; D015725:Fluconazole / Q000008:administration & dosage / Q000493:pharmacokinetics; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D020381:Interleukin-17 / Q000032:analysis; D053759:Interleukin-23 / Q000032:analysis; D008813:Mice, Inbred ICR; D011239:Prednisolone / Q000008:administration & dosage*
chemical_list: D000935:Antifungal Agents; D004967:Estrogens; D007166:Immunosuppressive Agents; D020381:Interleukin-17; D053759:Interleukin-23; D015725:Fluconazole; D011239:Prednisolone
keywords: Candida albicans; Mouse model; Mucosal candidiasis; Oral; Vaginal