title: Temporal-Spatial Resolution Fate Mapping Reveals Distinct Origins for Embryonic and Adult Microglia in Zebrafish.
abstract: Microglia are CNS resident macrophages, and they play important roles in neural development and function. Recent studies have suggested that murine microglia arise from a single source, the yolk sac (YS), yet these studies lack spatial resolution to define the bona fide source(s) for microglia. Here, using light-induced high temporal-spatial resolution fate mapping, we challenge this single-source view by showing that microglia in zebrafish arise from multiple sources. The embryonic/larval microglia originate from the rostral blood island (RBI) region, the equivalent of mouse YS for myelopoiesis, whereas the adult microglia arise from the ventral wall of dorsal aorta (VDA) region, a tissue also producing definitive hematopoiesis in mouse. We further show that the VDA-region-derived microglia are Runx1 dependent, but cMyb independent, and developmentally regulated differently from the RBI region-derived microglia. Our study establishes a new paradigm for investigating the development and function of distinct microglia populations.
authors.affiliation: Division of Life Science, State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Division of Life Science, State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Electronic and Computer Engineering, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC.
authors.forename: Sicong
authors.initials: S
authors.lastname: He
authors.affiliation: Division of Life Science, State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Division of Life Science, State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC.
authors.forename: Wan
authors.initials: W
authors.lastname: Jin
authors.affiliation: Division of Life Science, State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC.
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.affiliation: Department of Electronic and Computer Engineering, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC. Electronic address: eequ@ust.hk.
authors.forename: Jianan Y
authors.initials: JY
authors.lastname: Qu
authors.affiliation: Division of Life Science, State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PRC. Electronic address: zilong@ust.hk.
authors.forename: Zilong
authors.initials: Z
authors.lastname: Wen
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D018507:Gene Expression Regulation, Developmental; D006410:Hematopoiesis / Q000502:physiology*; D017403:In Situ Hybridization; D051379:Mice; D017628:Microglia / Q000166:cytology* / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology; D015017:Yolk Sac; D015027:Zebrafish / Q000254:growth & development* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029961:Zebrafish Proteins