title: Decitabine Improves the Clinical Manifestations of Rats With l-NAME-Induced Pre-eclampsia: A Potential Approach to Studying Pre-eclampsia.
abstract: OBJECTIVE
  Pre-eclampsia is a major cause of maternal mortality and morbidity. Conditions with low oxygen tension are regarded as a key factor. Decitabine can partly attenuate the effects of hypoxia. This research was designed to investigate the effects of decitabine in rats with NG-Nitro-L-arginine Methyl Eater (L-NAME) induced pre-eclampsia and to explore the molecular mechanisms.
  
  
  METHODS
  A Wistar rat model of pre-eclampsia was established by intraperitoneal injection of L-NAME, and the intervention reagent was decitabine. Blood pressure (BP) and 24-h urinary protein were monitored. The expression of Mammary Serine Protease Inhibitor (SERPINB5, maspin) in the placenta was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting.
  
  
  RESULTS
  Systolic BP in the tail artery of pregnant rats was increased by more than 30 mm Hg, and 24-h urinary protein was significantly increased after L-NAME was added. After decitabine treatment, blood pressure and 24-h urinary protein were significantly decreased. The expression of SERPINB5 in the placenta significantly increased after L-NAME was added. Decitabine significantly elevated the expression of SERPINB5 in the placenta of rats with L-NAME-induced preeclampsia.
  
  
  CONCLUSIONS
  Decitabine reduced 24-h urinary protein and partly decreased blood pressure of pre-eclampsia in late pregnancy in rats with L-NAME-induced pre-eclampsia and increased the expression of SERPINB5, but the molecular mechanism of decitabine's effect remains unknown. This research provided a potential approach to studying the pathogenesis, treatment and prevention of pre-eclampsia.
authors.affiliation: a Department of Obstetrics and Gynecology , Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China .
authors.forename: Xinwei
authors.initials: X
authors.lastname: Shi
authors.affiliation: b Department of Obstetrics and Gynecology , Shenzhen Baoan Maternal and Child Health Hospital , Shenzhen , China .
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: c Department of Urology , Wuhan Third Hospital , Guanggu Campus , Wuhan , China .
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: a Department of Obstetrics and Gynecology , Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China .
authors.forename: Fuyuan
authors.initials: F
authors.lastname: Qiao
authors.affiliation: a Department of Obstetrics and Gynecology , Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China .
authors.forename: Haiyi
authors.initials: H
authors.lastname: Liu
authors.affiliation: a Department of Obstetrics and Gynecology , Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China .
authors.forename: Xun
authors.initials: X
authors.lastname: Gong
authors.affiliation: a Department of Obstetrics and Gynecology , Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China .
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Li
authors.affiliation: d Department of Obstetrics , Hubei Women and Children Hospital , Wuhan , China , and.
authors.forename: Hui
authors.initials: H
authors.lastname: Du
authors.affiliation: a Department of Obstetrics and Gynecology , Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China .
authors.forename: Wanlu
authors.initials: W
authors.lastname: Liu
authors.affiliation: e Department of Obstetrics and Gynecology , Wuhan Women and Children Medical Care Center , Wuhan , China.
authors.forename: Guiju
authors.initials: G
authors.lastname: Tang
authors.affiliation: a Department of Obstetrics and Gynecology , Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China .
authors.forename: Jing
authors.initials: J
authors.lastname: Cao
authors.affiliation: a Department of Obstetrics and Gynecology , Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China .
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wu
keywords: SERPINB5; decitabine; placenta; pre-eclampsia