title: Biological effects of decabromodiphenyl ether (BDE209) and Pb on earthworm (Eisenia fetida) in a soil system.
abstract: BDE209 and Pb are ubiquitous contaminants at e-waste recycling sites (EWRSs). This study aimed to determine acute and sub-acute toxicity to earthworm Eisenia fetida induced by BDE209 and Pb in natural soil. Results demonstrated that the inhibition of Pb on growth and reproduction of earthworms followed a dose-dependent pattern. Earthworms exposed to 100 mg kg(-1) of BDE209 displayed avoidance responses, while the soil indicated a more obvious decline of habitat function with the increase of Pb level. Comet assay suggested that increasing concentrations of Pb exposure resulted in a gradual increase in the tail length and olive tail moment, which meant that the degree of DNA damage was promoted. BDE209 addition could reduce the damage; therefore the joint effects of both chemicals showed antagonistic. These results revealed that joint exposure (BDE209-Pb) could elicit pronounced biochemical and physiological responses in earthworms, and the DNA damage might be potential molecular biomarker of the two pollutants.
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai 200237, China; School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai 200237, China; School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: wzhang@ecust.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai 200237, China; School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai 200237, China; School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liang
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai 200237, China; School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Kuangfei
authors.initials: K
authors.lastname: Lin
mesh_terms: D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D015415:Biomarkers; D020552:Comet Assay; D004249:DNA Damage; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D007854:Lead / Q000633:toxicity*; D009835:Oligochaeta / Q000187:drug effects* / Q000254:growth & development; D059027:Recycling; D012098:Reproduction / Q000187:drug effects; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D055768:Halogenated Diphenyl Ethers; D012987:Soil; D012989:Soil Pollutants; D007854:Lead; C010902:decabromobiphenyl ether
keywords: BDE209-Pb; Biological effect; Combined pollution; DNA damage; Eisenia fetida