title: Molecular characterization of cystic echinococcosis: First record of G7 in Egypt and G1 in Yemen.
abstract: Few molecular studies have identified the current status of cystic echinococcosis in Egypt. The present study aimed to ascertain the genotype(s) of Echinococcus granulosus responsible for human hydatidosis in different Egyptian governorates (regions). Animal isolates were collected from 40 camels, 5 pigs and 44 sheep. 27 human isolates were included in the present study. Specific PCR was performed and followed by DNA sequencing for mitochondrial 12S ribosomal RNA gene and BLAST analysis.The sheep cysts were not hydatid cysts. G6 genotype (camel starin) predominates in human, camel and pig isolates. G7 genotype (pig strain) was detected in two human isolates and one pig isolate. G1 genotype (sheep strain) was detected in one human isolate from Yemen and in no animal isolates. This is the first record of G7 in Egypt and G1 in Yemen.
authors.forename: Yosra H
authors.initials: YH
authors.lastname: Alam-Eldin
authors.forename: Heba E
authors.initials: HE
authors.lastname: Abdel Aaty
authors.forename: Mona A
authors.initials: MA
authors.lastname: Ahmed
mesh_terms: D000818:Animals; D002162:Camelus; D016000:Cluster Analysis; D018509:DNA, Helminth / Q000235:genetics; D004443:Echinococcosis / Q000469:parasitology* / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004534:Egypt; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012756:Sheep; D013552:Swine; D015006:Yemen
chemical_list: D018509:DNA, Helminth; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S