title: Apolipoprotein A5 and apolipoprotein C3 single nucleotide polymorphisms are correlated with an increased risk of coronary heart disease: a case-control and meta-analysis study.
abstract: BACKGROUND
  Triglycerides (TGs) are proatherogenic lipoproteins involving the risk of coronary heart disease (CHD), while apolipoprotein A5 (APOA5) and apolipoprotein C3 (APOC3) are main lipoproteins composing TG-rich lipoproteins. In this study, we aim to explore the correlation of CHD with APOA5 -1131 T > C and APOC3 -455 T > C single nucleotide polymorphisms (SNPs).
  
  
  METHODS
  A sum of 210 CHD patients, hospitalized between Jan. 2013 and Mar. 2015 at China-Japan Union Hospital, Jilin University, were selected as our case group and 223 healthy individuals who had physical examination at same hospital at the same period were selected as control group. The frequency distribution of genotypes of APOA5 -1131 T > C and APOC3 -455 T > C SNPs were measured by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The Stata 12.0 software was utilized for statistical analyses.
  
  
  RESULTS
  There was no significant difference on age and sex between case and control group (P > 0.05). History of smoking, drinking, hypertension and diabetes mellitus, body mass index and levels of TG and fasting blood sugar in case group were shown to be higher than control group (P < 0.05), while levels of total cholesterol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol in case group were lower than control group (P < 0.05). Both CC and TC' + CC frequencies of APOA5 -1131 T > C and APOC3 -455 T > C in case group were higher compared to control group (both P < 0.05). Additionally, T allele frequencies of the two SNPs in case group were lower than control group, while C allele in case group has higher frequencies compared to control group (both P < 0.05). The results of meta-analysis under allele and dominant models showed that APOA5 -1131 T > C and APOC3 -455 T > C SNPs are likely to increase the risk of CHD (both P < 0.05).
  
  
  CONCLUSIONS
  APOA5 -1131 T > C and APOC3 -455 T > C SNPs may play potent roles in the development and progression of CHD.
authors.affiliation: Department of Molecular Biology, College of Basic Medical Science, Jilin University, Changchun, 130000, P.R. China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Internal Medicine-Cardiovascular, the Fourth Affiliated Hospital of China Medical University, Changchun, 130000, P.R. China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Lei
authors.affiliation: Department of Neurosurgery, FAW General Hospital, Jilin University, Changchun, 130000, P.R. China.
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Yu
authors.affiliation: Department of Cardiology, China-Japan Union Hospital, Jilin University, No. 126 Xiantai Stree, Changchun, 130000, P.R. China.
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Liu
authors.affiliation: Department of Cardiology, China-Japan Union Hospital, Jilin University, No. 126 Xiantai Stree, Changchun, 130000, P.R. China. anzhe@jlu.edu.cn.
authors.forename: Zhe
authors.initials: Z
authors.lastname: An
authors.affiliation: Department of Internal Medicine-Cardiovascular, the Fourth Affiliated Hospital of China Medical University, Changchun, 130000, P.R. China.
authors.forename: Chun-Li
authors.initials: CL
authors.lastname: Song
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D000072040:Apolipoprotein A-V; D053305:Apolipoprotein C-III / Q000097:blood / Q000235:genetics*; D001054:Apolipoproteins A / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D003327:Coronary Disease / Q000097:blood / Q000235:genetics* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D014280:Triglycerides / Q000097:blood
chemical_list: C437704:APOA5 protein, human; D000072040:Apolipoprotein A-V; D053305:Apolipoprotein C-III; D001054:Apolipoproteins A; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D014280:Triglycerides