title: Colorimetric and Phosphorimetric Dual-Signaling Strategy Mediated by Inner Filter Effect for Highly Sensitive Assay of Organophosphorus Pesticides.
abstract: We describe here a colorimetric and phosphorimetric dual-signaling strategy for sensitive assay of organophosphorus pesticides (OPPs). The principle for assay depends on the phenomenon that the phosphorescence of Mn-ZnS quantum dots (QDs) can be dramatically quenched by Au nanoparticles (AuNPs) through the inner filter effect (IFE) and the activity of acetylcholinesterase (AChE), an enzyme that catalytically hydrolyzes acetylthiocholine to thiocholine that can be inhibited by OPPs. By virtue of the variations of absorbance and phosphorescence of the analytical system, a dual-readout assay for OPPs has been proposed. The limits of detection for different OPPs including paraoxon, parathion, omethoate, and dimethyl dichlorovinyl phosphate (DDVP) are found to be 0.29, 0.59, 0.67, and 0.44 ng/L, respectively. The proposed assay was allowed to detect pesticides in real spiked samples and authentic contaminated apples with satisfactory results, suggesting its potential applications to detect pesticides in complicated samples.
authors.affiliation: Laboratory of Functionalized Molecular Solids, Ministry of Education, ‡Anhui Key Laboratory of Chemo/Biosensing, and §Laboratory of Optical Probes and Bioelectrocatalysis (LOPAB), College of Chemistry and Materials Science, Anhui Normal University , Wuhu 241000, P. R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Laboratory of Functionalized Molecular Solids, Ministry of Education, ‡Anhui Key Laboratory of Chemo/Biosensing, and §Laboratory of Optical Probes and Bioelectrocatalysis (LOPAB), College of Chemistry and Materials Science, Anhui Normal University , Wuhu 241000, P. R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Laboratory of Functionalized Molecular Solids, Ministry of Education, ‡Anhui Key Laboratory of Chemo/Biosensing, and §Laboratory of Optical Probes and Bioelectrocatalysis (LOPAB), College of Chemistry and Materials Science, Anhui Normal University , Wuhu 241000, P. R. China.
authors.forename: Junyong
authors.initials: J
authors.lastname: Sun
authors.affiliation: Laboratory of Functionalized Molecular Solids, Ministry of Education, ‡Anhui Key Laboratory of Chemo/Biosensing, and §Laboratory of Optical Probes and Bioelectrocatalysis (LOPAB), College of Chemistry and Materials Science, Anhui Normal University , Wuhu 241000, P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
mesh_terms: D003124:Colorimetry / Q000295:instrumentation / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D045663:Quantum Dots / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D009943:Organophosphorus Compounds; D010575:Pesticides
keywords: Au nanoparticles; Mn-ZnS quantum dots; acetylcholinesterase; colorimetric and phosphorimetric dual signaling; inner filter effect; organophosphorus pesticides