title: Comparison of biomass and lipid production under ambient carbon dioxide vigorous aeration and 3% carbon dioxide condition among the lead candidate Chlorella strains screened by various photobioreactor scales.
abstract: Chlorella species from the UTEX collection, classified by rDNA-based phylogenetic analysis, were screened based on biomass and lipid production in different scales and modes of culture. The lead candidate strains of C. sorokiniana UTEX 1230 and C. vulgaris UTEX 395 and 259 were compared between conditions of vigorous aeration with filtered atmospheric air and 3% CO2 shake-flask cultivation. The biomass of UTEX 1230 produced 2 times higher at 652 mg L(-1) dry weight under both ambient CO2 vigorous aeration and 3% CO2 conditions, while UTEX 395 and 259 under 3% CO2 increased to 3 times higher at 863 mg L(-1) dry weight than ambient CO2 vigorous aeration. The triacylglycerol contents of UTEX 395 and 259 increased more than 30 times to 30% dry weight with 3% CO2, indicating that additional CO2 is essential for both biomass and lipid accumulation in UTEX 395 and 259.
authors.affiliation: Department of Biochemistry, University of Nebraska-Lincoln, 1901 Vine Street, Lincoln, NE 68588, United States.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kobayashi
authors.affiliation: Department of Biochemistry, University of Nebraska-Lincoln, 1901 Vine Street, Lincoln, NE 68588, United States.
authors.forename: Austin
authors.initials: A
authors.lastname: Barnes
authors.affiliation: Department of Biochemistry, University of Nebraska-Lincoln, 1901 Vine Street, Lincoln, NE 68588, United States.
authors.forename: Travis
authors.initials: T
authors.lastname: Jensen
authors.affiliation: School of Biological Science, University of Nebraska-Lincoln, 1104 T Street, Lincoln, NE 68588, United States.
authors.forename: Eric
authors.initials: E
authors.lastname: Noel
authors.affiliation: Synaptic Research, 1448 South Rolling Road, Baltimore, MD 21227, United States.
authors.forename: Gunjan
authors.initials: G
authors.lastname: Andlay
authors.affiliation: Department of Chemical & Biomolecular Engineering, John Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, United States.
authors.forename: Julian N
authors.initials: JN
authors.lastname: Rosenberg
authors.affiliation: Department of Chemical & Biomolecular Engineering, John Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, United States.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Betenbaugh
authors.affiliation: National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401, United States.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Guarnieri
authors.affiliation: Department of Biochemistry, University of Nebraska-Lincoln, 1901 Vine Street, Lincoln, NE 68588, United States; Department of Chemical & Biomolecular Engineering, John Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, United States; Synaptic Research, 1448 South Rolling Road, Baltimore, MD 21227, United States.
authors.forename: George A
authors.initials: GA
authors.lastname: Oyler
mesh_terms: D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism*; D002708:Chlorella / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis*; D058433:Photobioreactors; D014280:Triglycerides / Q000378:metabolism
chemical_list: D008055:Lipids; D014280:Triglycerides; D002245:Carbon Dioxide
keywords: Biomass; Chlorella sorokiniana; Chlorella vulgaris; Lipid content; rDNA-based phylogenetic tree