title: 17β-Estradiol Stimulates Generation of Reactive Species Oxygen and Nitric Oxide in Ovarian Adenocarcinoma Cells (OVCAR 3).
abstract: BACKGROUND
  Experimental and epidemiological evidence supports a role for steroid hormones in the pathogenesis of ovarian cancer. Among steroid hormones, 17β-estradiol (E2) has the most potent effect on proliferation, apoptosis and metastasis.
  
  
  OBJECTIVE
  In the present study, we investigated the effect of E2 on production of ROS and NO in ovarian cancer cells.
  
  
  METHODS
  Ovarian adenocarcinoma cell line (OVCAR-3) was cultured and treated with various concentrations of E2, antioxidants (N-acetyle cysteine and Ebselen) and ICI182780 as an estrogen receptor antagonist. MTT test was performed to evaluate cell viability. NO and ROS levels were measured by Griess and DCFH-DA methods, respectively.
  
  
  RESULTS
  ROS levels as well as NO levels were increased in OVCAR-3 cells treated with E2. The increase in ROS production was in parallel with increased cell viability which indicates that estrogen-induced ROS can participate in cancer progression. ICI182780 abolished E2-induced ROS production. Progesterone was also effective in reducing ROS and NO generation.
  
  
  CONCLUSIONS
  NO and ROS are important molecules in signaling networks in cell. These molecules can be used as therapeutic targets for prevention and treatment of ovary cancer and other estrogen-induced malignancies.
authors.affiliation: Department of Biochemistry, Iran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Maleki
authors.affiliation: Department of Biochemistry, Iran University of Medical Sciences, Tehran, IR Iran ; Metabolic Disorders Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Mitra
authors.initials: M
authors.lastname: Nourbakhsh
authors.affiliation: Department of Biochemistry, Iran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shabani
authors.affiliation: Department of Biochemistry, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Korani
authors.affiliation: Department of Laboratory Sciences, Tabriz University of Medical Sciences, Tabriz, IR Iran.
authors.forename: Seyed Manuchehr
authors.initials: SM
authors.lastname: Nourazarian
authors.affiliation: Hematology-Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Ostadali Dahaghi
authors.affiliation: Departement of Laboratory, Labafi Nedjad Hospital, Social Security Organization, Tehran, IR Iran.
authors.forename: Mohamad Hossein
authors.initials: MH
authors.lastname: Moghadasi
keywords: 17β-estradiol; DCFH-DA; Ovarian Adenocarcinoma Cells; Reactive Oxygen Species