title: Effects of low-level laser irradiation on proliferation and functional protein expression in human RPE cells.
abstract: Low-level laser irradiation (LLLI) modulates a set of biological effects in many cell types such as fibroblasts, keratinocytes, and stem cells. However, no study to date has reported the effects of LLLI on retinal pigment epithelia (RPE) cells. The aim of this study was to investigate whether LLLI could enhance the proliferation of RPE cells and increase the expression of RPE functional genes/proteins. Human ARPE-19 cells were seeded overnight and treated with 8 J/cm(2) of LLLI. Cell proliferation was measured by CCK8 assay and cell cycle distribution was evaluated by FACS. The transcription of cell cycle-specific genes and RPE functional genes was quantified by RT-PCR. Moreover, the expression of ZO-1 and CRALBP were evaluated by immunostaining. A dose of 8 J/cm(2) of LLLI significantly increased proliferation and promoted cell cycle progression while upregulating the transcription of CDK4 and CCND1 and decreasing the transcription of CDKN2A, CDKN2C, and CDKN1B in human ARPE-19 cells. Additionally, LLLI enhanced the expression of ZO-1 and CRALBP in human ARPE-19 cells. In conclusion, LLLI could enhance the proliferative ability of human ARPE-19 cells by modulating cyclin D1, CDK4, and a group of cyclin-dependent kinase inhibitors. It also could increase the expression of RPE-specific proteins. Thus, LLLI may be a potential approach for the treatment of RPE degenerative diseases.
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, 1 East Jianshe Road, Erqi District, Zhengzhou, China.|Clinical Stem Cell Research Center, Peking University Third Hospital, 49 Huayuan North Road, Haidian District, Beijing, China.
authors.forename: Yalong
authors.initials: Y
authors.lastname: Dang
authors.affiliation: Clinical Stem Cell Research Center, Peking University Third Hospital, 49 Huayuan North Road, Haidian District, Beijing, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Wu
authors.affiliation: Clinical Stem Cell Research Center, Peking University Third Hospital, 49 Huayuan North Road, Haidian District, Beijing, China.|Clinical Lab of Tissue & Cell Research Center, Department of Biotech Treatment, Logistics College of Chinese People's Armed Police Force, Tianjin, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Ophthalmology, Yellow-River Hospital, Henan University of Science and Technology, Sanmenxia, China.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Mu
authors.affiliation: Department of Ophthalmology, Yellow-River Hospital, Henan University of Science and Technology, Sanmenxia, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.affiliation: Clinical Stem Cell Research Center, Peking University Third Hospital, 49 Huayuan North Road, Haidian District, Beijing, China.|Beijing No.4 High School, Beijing, China.
authors.forename: Haotian
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, 1 East Jianshe Road, Erqi District, Zhengzhou, China. asiadragon@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Clinical Stem Cell Research Center, Peking University Third Hospital, 49 Huayuan North Road, Haidian District, Beijing, China. zhangc1@yahoo.com.
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006801:Humans; D007834:Lasers; D011506:Proteins / Q000235:genetics*; D055213:Retinal Pigment Epithelium / Q000166:cytology* / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D011506:Proteins
keywords: Age-related macular degeneration; Cell proliferation; Low-level laser irradiation; Retinal pigment epithelium cells