title: Secreted frizzled-related protein promotors are hypermethylated in cutaneous squamous carcinoma compared with normal epidermis.
abstract: BACKGROUND
  The Wnt signaling pathway is abnormally activated in many human cancers. Secreted frizzled-related proteins (SFRPs) function as negative regulators of Wnt signaling and play an important role in carcinogenesis. SFRP promoter hypermethylation has often been identified in human cancers; however, the precise role of SFRPs in cutaneous squamous cell carcinoma (SCC) is unclear.
  
  
  METHODS
  The methylation status of the SFRP family was analyzed in an age-and sex-matched case-control study, including 40 cutaneous SCC cases and 40 normal controls, using the MassARRAY EpiTYPER system.
  
  
  RESULTS
  The methylation rate of SFRP1, SFRP2, SFRP4, and SFRP5 promoters was significantly higher in cutaneous SCC tissues than in adjacent tissue and normal skin samples.
  
  
  CONCLUSIONS
  Our manuscript mainly discussed the average methylation rate of SFRPs (SFRP1, SFRP2, SFRP4, and SFRP5) promoters are significantly high in tumor tissue samples and the average CpG island methylation rate among different pathological levels of cutaneous SCC between these genes are different.
  
  
  CONCLUSIONS
  Our findings suggest that promoter hypermethylation of SFRPs is associated with the development of carcinoma, and could be a useful tumor marker for cutaneous SCC and other types of cancers.
authors.affiliation: Department of Dermatology, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830000, China. junqinliang@163.com.
authors.forename: Junqin
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Dermatology, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830000, China. xiaojkang@163.com.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Kang
authors.affiliation: Department of Dermatology, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830000, China. yilinuerhalifu@163.com.
authors.forename: Yilinuer
authors.initials: Y
authors.lastname: Halifu
authors.affiliation: Department of Plastic surgery, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumchi, 830000, China. xuewen_zeng@163.com.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Zeng
authors.affiliation: School of Life Sciences, Northwest University, Xi'an, 710069, China. tianbojin1973@gmail.com.|National Engineering Research Center for Miniaturized Detection Systems, Xi'an, 710069, China. tianbojin1973@gmail.com.
authors.forename: Tianbo
authors.initials: T
authors.lastname: Jin
authors.affiliation: School of Life Sciences, Northwest University, Xi'an, 710069, China. zhmx9155@163.com.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Dermatology, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830000, China. lou_dong@163.com.
authors.forename: Dong
authors.initials: D
authors.lastname: Luo
authors.affiliation: Department of Dermatology, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830000, China. yuandingagent@163.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Dermatology, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830000, China. yunminzhou@163.com.
authors.forename: Yunmin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Dermatology, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830000, China. buwajierr@163.com.
authors.forename: Buwajier
authors.initials: B
authors.lastname: Yakeya
authors.affiliation: Department of Dermatology, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830000, China. dilinuerr@163.com.
authors.forename: Dilinuer
authors.initials: D
authors.lastname: Abudu
authors.affiliation: Department of Dermatology, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830000, China. xiongmipu@163.com.
authors.forename: Xiongming
authors.initials: X
authors.lastname: Pu
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics; D004817:Epidermis / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008875:Middle Aged; D005810:Multigene Family / Q000235:genetics; D011401:Promoter Regions, Genetic; D012878:Skin Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D060449:Wnt Signaling Pathway / Q000235:genetics; D000099274:Frizzled-Related Proteins
chemical_list: D006023:Glycoproteins; D047908:Intracellular Signaling Peptides and Proteins; D000099274:Frizzled-Related Proteins