title: Antitumor effect of COOH-terminal polypeptide of human TERT is associated with the declined expression of hTERT and NF-κB p65 in HeLa cells.
abstract: Human telomerase reverse transcriptase (hTERT) plays an important role in the development of tumors and has been investigated as a potent target for anticancer therapy. In the present study, we constructed a recombinant adenovirus, Ad-EGFP-C197 which was capable of expressing COOH‑terminal polypeptide of hTERT (amino acid 936-1,132, termed as C197 for the reason that it contains 197 amino acids). Infection of HeLa cells with Ad-EGFP-C197 suppressed the activity of telomerase, decreased the expression of hTERT and NF-κB p65, and induced rapid growth delay and apoptosis of HeLa cells in vitro. In nude mice xenografted with HeLa tumors, injection of Ad-EGFP-C197 into the tumor nodule significantly slowed tumor growth and promoted tumor cell apoptosis, as well as reduced the expression of NF-κB p65 in tumor tissues. In the present study, we suggest that the antitumor effect of C197 is associated with the declined expression of hTERT and NF-κB p65. Our results highlight the potential of C197 in tumor therapy.
authors.affiliation: Center for Drug Evaluation and Research, School of Pharmaceutical Sciences, Southern Medical University, Baiyun, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Xian
authors.initials: X
authors.lastname: Wu
authors.affiliation: Center for Drug Evaluation and Research, School of Pharmaceutical Sciences, Southern Medical University, Baiyun, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Chen
authors.affiliation: Center for Drug Evaluation and Research, School of Pharmaceutical Sciences, Southern Medical University, Baiyun, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Center for Drug Evaluation and Research, School of Pharmaceutical Sciences, Southern Medical University, Baiyun, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Baoping
authors.initials: B
authors.lastname: Xie
authors.affiliation: School of Biotechnology, Southern Medical University, Baiyun, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Li
authors.affiliation: Center for Drug Evaluation and Research, School of Pharmaceutical Sciences, Southern Medical University, Baiyun, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Pingzheng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Center for Drug Evaluation and Research, School of Pharmaceutical Sciences, Southern Medical University, Baiyun, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Yuchang
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Center for Drug Evaluation and Research, School of Pharmaceutical Sciences, Southern Medical University, Baiyun, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Pang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015316:Genetic Therapy; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D010455:Peptides / Q000235:genetics*; D011401:Promoter Regions, Genetic; D019098:Telomerase / Q000096:biosynthesis* / Q000235:genetics; D051996:Transcription Factor RelA / Q000096:biosynthesis* / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D023041:Xenograft Model Antitumor Assays
chemical_list: D004268:DNA-Binding Proteins; D010455:Peptides; D051996:Transcription Factor RelA; C509186:TERT protein, human; D019098:Telomerase