title: Cell culture on hydrophilicity-controlled silicon nitride surfaces.
abstract: Cell culture on silicon nitride membranes is required for atmospheric scanning electron microscopy, electron beam excitation assisted optical microscopy, and various biological sensors. Cell adhesion to silicon nitride membranes is typically weak, and cell proliferation is limited. We increased the adhesion force and proliferation of cultured HeLa cells by controlling the surface hydrophilicity of silicon nitride membranes. We covalently coupled carboxyl groups on silicon nitride membranes, and measured the contact angles of water droplets on the surfaces to evaluate the hydrophilicity. We cultured HeLa cells on the coated membranes and evaluated stretch of the cell. Cell migration and confluence were observed on the coated silicon nitride films. We also demonstrated preliminary observation result with direct electron beam excitation-assisted optical microscope.
authors.affiliation: Shizuoka University, Johoku 3-5-1, Naka, Hamamatsu, 432-8561, Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Masuda
authors.affiliation: Shizuoka University, Johoku 3-5-1, Naka, Hamamatsu, 432-8561, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Inami
authors.affiliation: Shizuoka University, Johoku 3-5-1, Naka, Hamamatsu, 432-8561, Japan.
authors.forename: Atsuo
authors.initials: A
authors.lastname: Miyakawa
authors.affiliation: Shizuoka University, Johoku 3-5-1, Naka, Hamamatsu, 432-8561, Japan. kawata@eng.shizuoka.ac.jp.
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Kawata
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D022001:Focal Adhesions; D006367:HeLa Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008566:Membranes; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods; D017655:Silicon Compounds / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D017655:Silicon Compounds; C032734:silicon nitride
keywords: Carboxyl group; Cell adhesion; HeLa cell; Silicon nitride; Surface modification