title: A novel self-assembled nano micelle as a highly efficient artificial peroxidase based on hexadecyl trimethyl ammonium bromide and cytochrome c.
abstract: A novel artificial peroxidase (AP) with highly catalytic efficiency was designed using hexadecyl trimethyl ammonium bromide (CTAB, 3 mM) nano-micelles and bovine heart cytochrome c (Cyt c, 0.5 µM) in 100 mM, pH 8.0 phosphate buffer at 25°C. The catalytic rate (kcat) and Michaelis-Menten (Km) of the AP were determined to be 0.311 ± 0.013 s(-1) and 8.64 ± 0.6 µM. The catalytic efficiency was 0.0360 ± 0.0020 µM(-1)s(-1) (about 50% the efficiency of native horseradish peroxidase). The Ultraviolet-visible spectrophotometer and Circular Dichroism techniques were applied to study the properties of the CTAB-Cyt c nano-micelle. Designed AP can be applied instead of native horseradish peroxidase.
authors.affiliation: School of Life Sciences, Henan University, Kaifeng 475000, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Henan University, Kaifeng 475000, China.
authors.forename: Yu-Shuai
authors.initials: YS
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Henan University, Kaifeng 475000, China.
authors.forename: Xiao-Lu
authors.initials: XL
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Henan University, Kaifeng 475000, China.
authors.forename: Fang-Yong
authors.initials: FY
authors.lastname: Geng
authors.affiliation: School of Life Sciences, Henan University, Kaifeng 475000, China.
authors.forename: Bao-Lin
authors.initials: BL
authors.lastname: Xiao
authors.affiliation: School of Life Sciences, Henan University, Kaifeng 475000, China.
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Xu
authors.affiliation: School of Life Sciences, Henan University, Kaifeng 475000, China.
authors.forename: Di
authors.initials: D
authors.lastname: Li
authors.affiliation: School of Life Sciences, Henan University, Kaifeng 475000, China.|Institute of Biotechnology, Henan University, Kaifeng 475000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hong
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Moosavi-Movahedi
mesh_terms: D040761:Biomimetic Materials / Q000138:chemical synthesis*; D002384:Catalysis; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D045304:Cytochromes c / Q000138:chemical synthesis*; D004789:Enzyme Activation; D004795:Enzyme Stability; D008422:Materials Testing; D008823:Micelles; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D009195:Peroxidase / Q000138:chemical synthesis*; D013379:Substrate Specificity
chemical_list: D002593:Cetrimonium Compounds; D008823:Micelles; D045304:Cytochromes c; D009195:Peroxidase; D000077286:Cetrimonium
keywords: CTAB; Catalytic efficiency; artificial peroxidase; cytochrome c; nano-micelle