title: Hepatic artery bridging lessens temporary ischemic injury to bile canaliculi.
abstract: OBJECTIVE
  To study whether transfer of blood between the right gastroepiploic artery and gastroduodenal artery could lessens the damage to bile canaliculi.
  
  
  METHODS
  Forty male Bama miniature pigs were divided into four groups as follows: a control group, two hepatic artery ischemia groups (1 h and 2 h), and a hepatic artery bridging group. The hemodynamics of the hepatic artery in the hepatic artery bridging group was measured using color Doppler ultrasound. Morphological changes in the bile canaliculus were observed by transmission electron microscopy. Cofilin, heat shock protein 27 and F-actin expression was detected by immunohistochemistry, Western blot, and real-time polymerase chain reaction. Terminal deoxynucleotidyl transferase-mediated nick end-labeling method was used to evaluate liver injury.
  
  
  RESULTS
  The hemodynamics was not changed in the hepatic artery bridging group. The microvilli in the bile canaliculus were impaired in the two hepatic artery ischemia groups. The down-regulation of cofilin and F-actin and up-regulation of heat shock protein 27 were observed in the two hepatic artery ischemia groups, while there were no significant differences between the control group and hepatic artery bridging group.
  
  
  CONCLUSIONS
  Hepatic artery ischemia aggravates damage to bile canaliculi, and this damage can be diminished by a hepatic artery bridging duct.
authors.affiliation: Jia-Zhong Wang, Yang Liu, Jin-Long Wang, Le Lu, Ya-Fei Zhang, Hong-Wei Lu, Yi-Ming Li, Department of General Surgery, the Second Afﬁliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an 710004, Shaanxi Province, China.
authors.forename: Jia-Zhong
authors.initials: JZ
authors.lastname: Wang
authors.affiliation: Jia-Zhong Wang, Yang Liu, Jin-Long Wang, Le Lu, Ya-Fei Zhang, Hong-Wei Lu, Yi-Ming Li, Department of General Surgery, the Second Afﬁliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an 710004, Shaanxi Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Jia-Zhong Wang, Yang Liu, Jin-Long Wang, Le Lu, Ya-Fei Zhang, Hong-Wei Lu, Yi-Ming Li, Department of General Surgery, the Second Afﬁliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an 710004, Shaanxi Province, China.
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Wang
authors.affiliation: Jia-Zhong Wang, Yang Liu, Jin-Long Wang, Le Lu, Ya-Fei Zhang, Hong-Wei Lu, Yi-Ming Li, Department of General Surgery, the Second Afﬁliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an 710004, Shaanxi Province, China.
authors.forename: Le
authors.initials: L
authors.lastname: Lu
authors.affiliation: Jia-Zhong Wang, Yang Liu, Jin-Long Wang, Le Lu, Ya-Fei Zhang, Hong-Wei Lu, Yi-Ming Li, Department of General Surgery, the Second Afﬁliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an 710004, Shaanxi Province, China.
authors.forename: Ya-Fei
authors.initials: YF
authors.lastname: Zhang
authors.affiliation: Jia-Zhong Wang, Yang Liu, Jin-Long Wang, Le Lu, Ya-Fei Zhang, Hong-Wei Lu, Yi-Ming Li, Department of General Surgery, the Second Afﬁliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an 710004, Shaanxi Province, China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Lu
authors.affiliation: Jia-Zhong Wang, Yang Liu, Jin-Long Wang, Le Lu, Ya-Fei Zhang, Hong-Wei Lu, Yi-Ming Li, Department of General Surgery, the Second Afﬁliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an 710004, Shaanxi Province, China.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Li
mesh_terms: D051339:Actin Depolymerizing Factors / Q000235:genetics / Q000378:metabolism; D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001648:Bile Canaliculi / Q000098:blood supply / Q000378:metabolism / Q000648:ultrastructure*; D015415:Biomarkers / Q000097:blood; D001783:Blood Flow Velocity; D004195:Disease Models, Animal; D024405:Gastroepiploic Artery / Q000503:physiopathology / Q000601:surgery*; D005786:Gene Expression Regulation; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006499:Hepatic Artery / Q000000981:diagnostic imaging / Q000503:physiopathology*; D007511:Ischemia / Q000097:blood / Q000000981:diagnostic imaging / Q000235:genetics / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D008102:Liver Circulation; D008297:Male; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors; D018615:Ultrasonography, Doppler, Color
chemical_list: D051339:Actin Depolymerizing Factors; D000199:Actins; D015415:Biomarkers; D055551:HSP27 Heat-Shock Proteins
keywords: Cofilin; F-actin; Heat shock protein 27; Hepatic artery bridging; Hepatic artery ischemia; Nonanastomotic biliary strictures