title: Pri-Mir-34b/C and Tp-53 Polymorphisms are Associated With The Susceptibility of Papillary Thyroid Carcinoma: A Case-Control Study.
abstract: Tumor suppressor p53 directly regulated the abundance of the miR-34b/c. The interaction might contribute to certain cancer. We hypothesized that rs4938723 in the promoter region of pri-miR-34b/c and TP-53 Arg72Pro may be related to the risk of papillary thyroid carcinoma (PTC). A total of 784 patients with PTC and 1006 healthy controls were recruited to participate in this study. The variants were discriminated using a polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP). Additionally, the relative expression levels of miR-34b/c and TP-53 in 44 paired samples were revealed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). A significantly increased risk of PTC was observed in the miR-34b/c rs4938723 CT, CC, and CT/CC genotypes compared with the TT genotype (CT vs TT: adjusted odds ratio [OR] = 1.51, 95%confidence interval [CI] = 1.23-1.85; CC vs TT: adjusted OR = 1.89, 95%CI = 1.39-2.63; CT/CC vs TT: adjusted OR = 1.59, 95%CI = 1.30-1.92, respectively). Significantly increased PTC susceptibility was also associated with the TP-53 Arg72Pro CC and CG/CC genotypes compared with the GG genotype (CC vs GG: adjusted OR = 2.04, 95%CI = 1.54-2.70; CG/CC vs GG: adjusted OR = 1.35, 95%CI = 1.11-1.67, respectively). Stratification analysis revealed that patients carrying the TP-53 Arg72Pro C allele and CC genotype had a significantly increased risk for developing N1 (C vs. G: OR = 1.27, 95%CI = 1.03-1.56; CC vs. GG: OR = 1.62, 95%CI = 1.07-2.46, respectively). Combined analysis showed that the genotypes of rs4938723 CT/CC + TP-53CG/CC increased the risk of PTC compared with rs4938723TT + TP-53GG (OR = 2.25, 95%CI = 1.67-3.03). Additionally, level of miR-34b was significantly upregulated in PTC patients.These findings indicate that the miR-34b/c rs4938723 and TP-53 Arg72Pro polymorphisms may contribute to the susceptibility of PTC.
authors.affiliation: From the Department of Forensic Biology, West China School of Preclinical and Forensic Medicine (PC, YP, PB, LZ); Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children's Health, Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan (PC, RS, FY, YL, BZ,YW,LZ,LG); Central Laboratory, Yunnan University of Chinese Traditional Medicine, Kunming, Yunnan (RS); Department of Thyroid and Breast Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, P.R. China (YS, JZ).
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.forename: Ruifen
authors.initials: R
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Pu
authors.forename: Peng
authors.initials: P
authors.lastname: Bai
authors.forename: Fang
authors.initials: F
authors.lastname: Yuan
authors.forename: Yundan
authors.initials: Y
authors.lastname: Liang
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Wang
authors.forename: Yinghe
authors.initials: Y
authors.lastname: Sun
authors.forename: Jingqiang
authors.initials: J
authors.lastname: Zhu
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Linbo
authors.initials: L
authors.lastname: Gao
mesh_terms: D000328:Adult; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002291:Carcinoma, Papillary; D016022:Case-Control Studies; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: C522425:MIRN34 microRNA, human; D035683:MicroRNAs; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53