title: Gaining mass: the structure of respiratory complex I-from bacterial towards mitochondrial versions.
abstract: The 1MDa, 45-subunit proton-pumping NADH-ubiquinone oxidoreductase (complex I) is the largest complex of the mitochondrial electron transport chain. The molecular mechanism of complex I is central to the metabolism of cells, but has yet to be fully characterized. The last two years have seen steady progress towards this goal with the first atomic-resolution structure of the entire bacterial complex I, a 5Å cryo-electron microscopy map of bovine mitochondrial complex I and a ∼3.8Å resolution X-ray crystallographic study of mitochondrial complex I from yeast Yarrowia lipotytica. In this review we will discuss what we have learned from these studies and what remains to be elucidated.
authors.affiliation: Institute of Science and Technology Austria (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria.
authors.forename: James A
authors.initials: JA
authors.lastname: Letts
authors.affiliation: Institute of Science and Technology Austria (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria. Electronic address: sazanov@ist.ac.at.
authors.forename: Leonid A
authors.initials: LA
authors.lastname: Sazanov
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D002417:Cattle; D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray; D042967:Electron Transport Complex I / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism; D011487:Protein Conformation; D016963:Thermus thermophilus / Q000378:metabolism; D025062:Yarrowia / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D005656:Fungal Proteins; D042967:Electron Transport Complex I