title: Quinolone resistance and ornithine decarboxylation activity in lactose-negative Escherichia coli.
abstract: Quinolones and fluoroquinolones are widely used to treat uropathogenic Escherichia coli infections. Bacterial resistance to these antimicrobials primarily involves mutations in gyrA and parC genes. To date, no studies have examined the potential relationship between biochemical characteristics and quinolone resistance in uropathogenic E. coli strains. The present work analyzed the quinolone sensitivity and biochemical activities of fifty-eight lactose-negative uropathogenic E. coli strains. A high percentage of the isolates (48.3%) was found to be resistant to at least one of the tested quinolones, and DNA sequencing revealed quinolone resistant determining region gyrA and parC mutations in the multi-resistant isolates. Statistical analyses suggested that the lack of ornithine decarboxylase (ODC) activity is correlated with quinolone resistance. Despite the low number of isolates examined, this is the first study correlating these characteristics in lactose-negative E. coli isolates.
authors.affiliation: Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil .
authors.forename: Franciane
authors.initials: F
authors.lastname: Gomig
authors.affiliation: Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil .
authors.forename: Carolina Weigert
authors.initials: CW
authors.lastname: Galvão
authors.affiliation: Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil .
authors.forename: Denis Leandro de
authors.initials: DL
authors.lastname: Freitas
authors.affiliation: Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil .
authors.forename: Larissa
authors.initials: L
authors.lastname: Labas
authors.affiliation: Departamento de Química, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil .
authors.forename: Rafael Mazer
authors.initials: RM
authors.lastname: Etto
authors.affiliation: Departamento de Análises Clínicas, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil .
authors.forename: Luiz Antonio
authors.initials: LA
authors.lastname: Esmerino
authors.affiliation: Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brazil .
authors.forename: Marcelo Andrade de
authors.initials: MA
authors.lastname: Lima
authors.affiliation: Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil .
authors.forename: Marcia Helena
authors.initials: MH
authors.lastname: Appel
authors.affiliation: Departamento de Patologia Básica, Universidade Federal do Paraná, Curitiba, PR, Brazil .
authors.forename: Silvio Marques
authors.initials: SM
authors.lastname: Zanata
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba, PR, Brazil .
authors.forename: Maria Berenice Reynaud
authors.initials: MB
authors.lastname: Steffens
authors.affiliation: Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brazil .
authors.forename: Helena Bonciani
authors.initials: HB
authors.lastname: Nader
authors.affiliation: Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil .
authors.forename: Rafael Bertoni da
authors.initials: RB
authors.lastname: Silveira
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001938:Brazil; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics; D003653:Decarboxylation / Q000235:genetics / Q000502:physiology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000382:microbiology; D024841:Fluoroquinolones / Q000627:therapeutic use*; D006801:Humans; D007785:Lactose / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D009268:Nalidixic Acid / Q000627:therapeutic use*; D009952:Ornithine / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000235:genetics*; D014552:Urinary Tract Infections / Q000188:drug therapy* / Q000382:microbiology; D056689:Uropathogenic Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D009268:Nalidixic Acid; D009952:Ornithine; D009955:Ornithine Decarboxylase; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase; D007785:Lactose
keywords: ODC; gyrA; parC; uropathogenic