title: Effect of Mini-Tyrosyl-tRNA Synthetase/Mini-Tryptophanyl-tRNA Synthetase on Angiogenesis in Rhesus Monkeys after Acute Myocardial Infarction.
abstract: OBJECTIVE
  The purpose of this study was to clarify the effect of mini-tyrosyl-tRNA synthetase/mini-tryptophanyl-tRNA synthetase (mini-TyrRS/mini-TrpRS) in ischemic angiogenesis in rhesus monkeys with acute myocardial infarction (AMI).
  
  
  METHODS
  A 27-gauge needle was incorporated percutaneously into the left ventricular myocardium of rhesus monkeys with AMI. All monkeys were randomized to receive adenoviral vector mini-TyrRS/mini-TrpRS, which was administered as five injections into the infarcted myocardium, or saline or ad-null (control groups). The injections were guided by EnSite NavX left ventricular electroanatomical mapping. Mini-TyrRS/mini-TrpRS proteins were detected by Western blot and immunoprecipitation analyses. Microvessel density (MVD) per section was measured using immunostaining with a CD34 monoclonal antibody. Proliferating cardiomyocytes were identified through histological and immunohistochemical analyses. Myocardial perfusion and cardiac function were estimated by G-SPECT. Infarction size was also measured.
  
  
  RESULTS
  Western blot analyses showed that compared to the normal zone, the expression level of mini-TyrRS/mini-TrpRS was significantly different in the infarction zone. G-SPECT analysis indicated that the mini-TyrRS group had better cardiac function and myocardial perfusion after the injection of ad-mini-TyrRS than before, while mini-TrpRS injection had a totally opposite effect. After mini-TyrRS was administered, there was less of an infarction zone and more proliferating cardiomyocytes and capillaries in the mini-TyrRS group compared to both of the control groups, and the ad-mini-TrpRS group had a totally opposite effect.
  
  
  CONCLUSIONS
  These results indicated that angiogenesis could be either stimulated by mini-TyrRS or inhibited by mini-TrpRS.
authors.affiliation: Department of Cardiology, West China Hospital, School of Clinic Medicine, Sichuan University, Chengdu, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zeng
authors.affiliation: Department of Cardiology, West China Hospital, School of Clinic Medicine, Sichuan University, Chengdu, China.
authors.forename: Mian
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Cardiology, West China Hospital, School of Clinic Medicine, Sichuan University, Chengdu, China.
authors.forename: Gui-ying
authors.initials: GY
authors.lastname: You
authors.affiliation: Department of Nephrology, West China Hospital, School of Clinic Medicine, Sichuan University, Chengdu, China.
authors.forename: Rong-zheng
authors.initials: RZ
authors.lastname: Yue
authors.affiliation: Department of Cardiology, West China Hospital, School of Clinic Medicine, Sichuan University, Chengdu, China.
authors.forename: Yu-cheng
authors.initials: YC
authors.lastname: Chen
authors.affiliation: Department of Cardiology, West China Hospital, School of Clinic Medicine, Sichuan University, Chengdu, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Laboratory of Peptides Related with Human Diseases, National Laboratory of Biomedicine, Sichuan University, Chengdu, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Laboratory of Peptides Related with Human Diseases, National Laboratory of Biomedicine, Sichuan University, Chengdu, China.
authors.forename: Ou
authors.initials: O
authors.lastname: Qiang
authors.affiliation: Department of Cardiology, West China Hospital, School of Clinic Medicine, Sichuan University, Chengdu, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D002196:Capillaries / Q000201:enzymology* / Q000503:physiopathology; D049109:Cell Proliferation; D003326:Coronary Circulation; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D008253:Macaca mulatta; D008297:Male; D009203:Myocardial Infarction / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D032383:Myocytes, Cardiac / Q000201:enzymology / Q000473:pathology; D018919:Neovascularization, Physiologic; D010446:Peptide Fragments / Q000096:biosynthesis* / Q000235:genetics; D020127:Recovery of Function; D014369:Tryptophan-tRNA Ligase / Q000096:biosynthesis* / Q000235:genetics; D014448:Tyrosine-tRNA Ligase / Q000096:biosynthesis* / Q000235:genetics; D016277:Ventricular Function, Left
chemical_list: D018952:Antigens, CD34; D010446:Peptide Fragments; D014448:Tyrosine-tRNA Ligase; D014369:Tryptophan-tRNA Ligase
keywords: Acute myocardial infarction; Angiogenesis; Mini-TrpRS; Mini-TyrRS; Rhesus monkeys