title: Sensitive and selective determination of GSH based on the ECL quenching of Ru(II) 1,10-phenanthroline-5,6-dione complex.
abstract: Electrochemiluminescence (ECL) material Ru-dpq (Ru(bpy)2dpq(2+), dpq=1,10-phenanthroline-5,6-dione; bpy=2,2'-bipyridine) is found to be produced strong and stable anodic ECL signal, which could be quenched by reduced glutathione (GSH) and exhibits high sensitivity and selectivity simultaneously. According to the mass spectra of Ru-SG (product of Ru-dpq reacted with GSH), and single crystal structure of the final oxidized product Ru-dcbpy ((Ru(bpy)2dcbpy(2+), dcbpy=3,3-dicarboxy-2,2-bipyridine), we propose a new interacted mechanism between Ru-dpq and GSH. A good linear relation is estimated to be from 0.1 pM to 50 μM in the presence of calcium ion and the detection limit is as low as 0.087 pM (with the signal-to-noise ratio of 3). The relative standard deviation is 2.3% (for three repeated measurements). Furthermore, the ECL signal of Ru-dpq under a constant potential (1.2V) is extremely stable and the intensity could be maintained over 600 s, which promotes us to determine the concentration of GSH via chronoamperometry.
authors.affiliation: College of Chemistry, Sichuan University, No. 29 Wangjiang Road, Chengdu, PR China.
authors.forename: Yanxue
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Chemistry, Sichuan University, No. 29 Wangjiang Road, Chengdu, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: The State Key Laboratory for Biotherapy/Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Chemistry, Sichuan University, No. 29 Wangjiang Road, Chengdu, PR China.
authors.forename: Zhaoyu
authors.initials: Z
authors.lastname: Jin
authors.affiliation: College of Chemical Engineering, Sichuan University, No. 29 Wangjiang Road, Chengdu, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: College of Chemistry, Sichuan University, No. 29 Wangjiang Road, Chengdu, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.affiliation: College of Chemical Engineering, Sichuan University, No. 29 Wangjiang Road, Chengdu, PR China. Electronic address: xiaodan@scu.edu.cn.
authors.forename: Dan
authors.initials: D
authors.lastname: Xiao
mesh_terms: D003217:Conductometry / Q000295:instrumentation*; D056831:Coordination Complexes / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005978:Glutathione / Q000032:analysis* / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation*; D015203:Reproducibility of Results; D012428:Ruthenium / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D056831:Coordination Complexes; C000594168:phenanthroline-5,6-dione trithiacyclononane ruthenium(II); D012428:Ruthenium; D005978:Glutathione
keywords: Electrochemiluminescence; GSH; Ruthenium (II) complex