title: Involvement of SARA in Axon and Dendrite Growth.
abstract: SARA (Smad Anchor for Receptor Activation) plays a crucial role in Rab5-mediated endocytosis in cell lines localizing to early endosomes where it regulates morphology and function. Here, we analyzed the role of SARA during neuronal development and tested whether it functions as a regulator of endocytic trafficking of selected axonal and membrane proteins. Suppression of SARA perturbs the appearance of juxtanuclear endocytic recycling compartments and the neurons show long axons with large growth cones. Furthermore, surface distribution of the cell adhesion molecule L1 in axons and the fusion of vesicles containing transferring receptor (TfR) in dendrites were increased in neurons where SARA was silenced. Conversely, SARA overexpression generated large early endosomes and reduced neurite outgrowth. Taken together, our findings suggest a significant contribution of SARA to key aspects of neuronal development, including neurite formation.
authors.affiliation: Laboratorio Neurobiología, INIMEC-CONICET, Córdoba, Argentina.
authors.forename: Cristina Isabel
authors.initials: CI
authors.lastname: Arias
authors.affiliation: Laboratorio Neurobiología, INIMEC-CONICET, Córdoba, Argentina; Universidad Nacional de Córdoba, Córdoba, Argentina; Instituto Universitario de Ciencias Biomédicas de Córdoba, Córdoba, Argentina.
authors.forename: Sebastián Omar
authors.initials: SO
authors.lastname: Siri
authors.affiliation: Laboratorio Neurobiología, INIMEC-CONICET, Córdoba, Argentina; Universidad Nacional de Córdoba, Córdoba, Argentina; Instituto Universitario de Ciencias Biomédicas de Córdoba, Córdoba, Argentina.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Conde
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D001369:Axons / Q000378:metabolism*; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism*; D004705:Endocytosis; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism; D055495:Neurogenesis; D051381:Rats; D011990:Receptors, Transferrin / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D039842:Neural Cell Adhesion Molecule L1; D011990:Receptors, Transferrin; C000602391:Zfyve9 protein, rat