title: Elucidating the function and tolerance mechanism of gamma delta (γ δ) T cells in a Helicobacter pylori infection model.
abstract: In this study, the functions and mechanisms of γ δ T cells were analyzed in patients infected with Helicobacter pylori. Peripheral blood was collected from gastritis patients in the Gastroenterology Department of Ningbo No. 2 Hospital. Preliminary analyses revealed 24 H. pylori-positive and 17 H. pylori-negative patients. The wild-type and γ δ T knockout mice were infected with cultured H. pylori cells (obtained from the H. pylori-positive patients). H. pylori in mice was quantified by polymerase chain reaction; gastritis was confirmed by hematoxylin and eosin staining. The TCR-δ(-/-) mice were treated with vein adoptive immunotherapy 24 h prior to H. pylori inoculation; the same method was used to detect the extent of gastritis and bacterial colonization. The γ δ T knockout mice showed high levels of H. pylori infection than the wild-type mice; in addition, the knockout mice showed severe disease pathology. γ δ T knockout mice also displayed increased matrix metalloproteinase-9 (MMP-9) and decreased MMP-7 expression in the gastric mucosa. γ δ T cells play a protective role in patients infected with H. pylori. γ δ T cell [responsible for the production of interleukin-17 (IL-17) and IL-22] expression was increased in H. pylori-positive patients, indicating statistical significance. However, there was no significant difference in interferon-gamma + γ δ T expression between the positive and negative patients. This study demonstrated the probable involvement of γ δ T cells in the immune response of an organism, via the secretion of IL-17 and IL-22.
authors.affiliation: Critical Care Medical Center, Ningbo No. 2 Hospital, Ningbo, China.
authors.forename: T E
authors.initials: TE
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Ningbo No. 2 Hospital, Ningbo, China XiaominMedicine@163.com.
authors.forename: X M
authors.initials: XM
authors.lastname: Xu
authors.affiliation: Critical Care Medical Center, Ningbo No. 2 Hospital, Ningbo, China.
authors.forename: P
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Microbiology, Ningbo No. 2 Hospital, Ningbo, China.
authors.forename: S Y
authors.initials: SY
authors.lastname: Liang
authors.affiliation: Critical Care Medical Center, Ningbo No. 2 Hospital, Ningbo, China.
authors.forename: W X
authors.initials: WX
authors.lastname: Lv
mesh_terms: D000818:Animals; D005753:Gastric Mucosa / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000628:therapy; D005786:Gene Expression Regulation; D016481:Helicobacter Infections / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000628:therapy; D016480:Helicobacter pylori / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D007108:Immune Tolerance; D016219:Immunotherapy, Adoptive; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology; D007378:Interleukins / Q000235:genetics / Q000276:immunology; D020783:Matrix Metalloproteinase 7 / Q000235:genetics / Q000276:immunology; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D012720:Severity of Illness Index; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology / Q000473:pathology / Q000637:transplantation; D000097043:Interleukin-22
chemical_list: D007371:Interferon-gamma; D020381:Interleukin-17; D007378:Interleukins; D020783:Matrix Metalloproteinase 7; D020780:Matrix Metalloproteinase 9; D016692:Receptors, Antigen, T-Cell, gamma-delta; D000097043:Interleukin-22