title: Mechanism study for hypoxia induced differentiation of insulin-producing cells from umbilical cord blood-derived mesenchymal stem cells.
abstract: Recently, we have successfully obtained functional IPCs efficiently from umbilical cord blood-derived mesenchymal stem cells by using hypoxia treatment. In this study, we further elaborated that the improved function and viability of IPCs are the result of the interaction β cell development pathway and c-Met/HGF axis induced by hypoxia. We found that hypoxia induced c-MET elevation is efficiently initiated the early stage differentiation IPCs from MSCs, and HGF improved the fully differentiation of IPCs by inducing the expression of NGN3. This finding may contribute to understanding β cell development and the development of stem cell therapy for diabetes.
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China; Institute of Microbiology, Seoul National University, Seoul 151-742, South Korea. Electronic address: sunbo@seu.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
authors.forename: Xian-Hui
authors.initials: XH
authors.lastname: Meng
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: School of Geography and Biological Information, Nanjing University of Posts and Telecommunications, Nanjing 210046, PR China.
authors.forename: Shancheng
authors.initials: S
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
authors.forename: Zhong-Dang
authors.initials: ZD
authors.lastname: Xiao
mesh_terms: D002454:Cell Differentiation; D015687:Cell Hypoxia; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D066298:In Vitro Techniques; D007231:Infant, Newborn; D007328:Insulin / Q000096:biosynthesis; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D019859:Proto-Oncogene Proteins c-met / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007328:Insulin; D019859:Proto-Oncogene Proteins c-met
keywords: Differentiation; HGF; Hypoxic; Mesenchymal stem cells; c-Met