title: Characterisation and Expression Analysis of MrLip1, a Class 3 Family Lipase of Malassezia restricta.
abstract: The genus Malassezia is associated with a wide range of skin diseases and is the predominant fungal genus isolated from human skin. Of the 14 Malassezia species identified, M. restricta is the most abundant fungal species found from both healthy and diseased skin. Emerging evidences have suggested that extracellular lipases of Malassezia play a critical role in its survival on the host skin surface. This study aimed to characterise the lipase 1 homologue (MrLip1) in M. restricta and to analyse its expression under different environmental conditions. The full sequence of the gene encoding MrLip1 was determined by rapid amplification of cDNA ends, and it was then heterologously expressed in Pichia pastoris. MrLip1 protein was successfully purified and used for lipase assay and specific antibody generation for use in expression analysis. The optimum pH and temperature for the activity of purified MrLip1 were pH 5.0 and 34 °C respectively. Furthermore, the expression of MrLip1 peaked at a similar pH and temperature, suggesting that the optimal conditions for MrLip1 protein activity and expression are similar to that found on the human skin surface. This study provides data to improve our understanding of the role and characteristics of lipase 1 in M. restricta.
authors.affiliation: Department of Systems Biotechnology, Chung-Ang University, Anseong, Korea.
authors.forename: Minji
authors.initials: M
authors.lastname: Park
authors.affiliation: Department of Systems Biotechnology, Chung-Ang University, Anseong, Korea.
authors.forename: Won Hee
authors.initials: WH
authors.lastname: Jung
authors.affiliation: Department of Dermatology, School of Medicine, Konkuk University, Seoul, Korea.
authors.forename: Song Hee
authors.initials: SH
authors.lastname: Han
authors.affiliation: Department of Chemistry, KAIST, Daejeon, Korea.
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of Dermatology, School of Medicine, Konkuk University, Seoul, Korea.|Research Institute of Medical Science, Konkuk University, Seoul, Korea.
authors.forename: Yang Won
authors.initials: YW
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D012628:Dermatitis, Seborrheic / Q000382:microbiology; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008290:Malassezia / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data
chemical_list: D018076:DNA, Complementary; D005656:Fungal Proteins; D008049:Lipase
keywords: Malassezia restricta; lipase; pH; skin; temperature