title: Generation and analysis of expressed sequence tags (ESTs) of Camelina sativa to mine drought stress-responsive genes.
abstract: Camelina sativa is an oil-producing crop belonging to the family of Brassicaceae. Due to exceptionally high content of omega fatty acid, it is commercially grown around the world as edible oil, biofuel, and animal feed. A commonly referred 'false flax' or gold-of-pleasure Camelina sativa has been interested as one of biofuel feedstocks. The species can grow on marginal land due to its superior drought tolerance with low requirement of agricultural inputs. This crop has been unexploited due to very limited transcriptomic and genomic data. Use of gene-specific molecular markers is an important strategy for new cultivar development in breeding program. In this study, Illumina paired-end sequencing technology and bioinformatics tools were used to obtain expression profiling of genes responding to drought stress in Camelina sativa BN14. A total of more than 60,000 loci were assembled, corresponding to approximately 275 K transcripts. When the species was exposed to 10 kPa drought stress, 100 kPa drought stress, and rehydrated conditions, a total of 107, 2,989, and 982 genes, respectively, were up-regulated, while 146, 3,659, and 1189 genes, respectively, were down-regulated compared to control condition. Some unknown genes were found to be highly expressed under drought conditions, together with some already reported gene families such as senescence-associated genes, CAP160, and LEA under 100 kPa soil water condition, cysteine protease, 2OG, Fe(II)-dependent oxygenase, and RAD-like 1 under rehydrated condition. These genes will be further validated and mapped to determine their function and loci. This EST library will be favorably applied to develop gene-specific molecular markers and discover genes responsible for drought tolerance in Camelina species.
authors.affiliation: Chungnam National University, Yuseong-gu, Daejeon, 305-764, Republic of Korea.
authors.forename: Bashistha Kumar
authors.initials: BK
authors.lastname: Kanth
authors.affiliation: Chungnam National University, Yuseong-gu, Daejeon, 305-764, Republic of Korea.
authors.forename: Shipra
authors.initials: S
authors.lastname: Kumari
authors.affiliation: Chungnam National University, Yuseong-gu, Daejeon, 305-764, Republic of Korea.
authors.forename: Seo Hee
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Chungnam National University, Yuseong-gu, Daejeon, 305-764, Republic of Korea.
authors.forename: Hye-Jeong
authors.initials: HJ
authors.lastname: Ha
authors.affiliation: Chungnam National University, Yuseong-gu, Daejeon, 305-764, Republic of Korea. Electronic address: gjlee@cnu.ac.kr.
authors.forename: Geung-Joo
authors.initials: GJ
authors.lastname: Lee
mesh_terms: D019607:Brassicaceae / Q000235:genetics* / Q000502:physiology; D057225:Data Mining; D055864:Droughts; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D063990:Gene Ontology; D017343:Genes, Plant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D018749:RNA, Plant / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics; D059467:Transcriptome
chemical_list: D005819:Genetic Markers; D018749:RNA, Plant
keywords: Biofuel; Camelina sativa; De novo assembly; Drought tolerance; EST; False flax; Marker; SSR