title: Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide.
abstract: We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive monitoring of hydrogen sulfide based on a dicyanoisophorone platform. An excellent linear relationship of fluorescence intensity ratio (I637/I558) (R(2) = 0.9867) versus hydrogen sulfide concentration in the range of 1-12 μM was obtained. This probe exhibited a remarkable fluorescence response to hydrogen sulfide over other physiological thiols or biological species, which fluoresces in the red region with a large Stokes shift (172 nm). This probe was successfully utilized to monitor H2S under in vitro physiological conditions and for imaging H2S in living cells and living zebrafish in vivo.
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing 210023, China. yongqian@nju.edu.cn zhuhl@nju.edu.cn.
authors.forename: Tianbao
authors.initials: T
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Lin
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Yuning
authors.initials: Y
authors.lastname: Shen
authors.forename: Hailiang
authors.initials: H
authors.lastname: Zhu
authors.forename: Yong
authors.initials: Y
authors.lastname: Qian
mesh_terms: D000818:Animals; D003512:Cyclohexanones / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000737:chemistry*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D009570:Nitriles / Q000737:chemistry*; D015027:Zebrafish
chemical_list: D003512:Cyclohexanones; D005456:Fluorescent Dyes; D009570:Nitriles; C005940:isophorone; D006862:Hydrogen Sulfide