title: Wogonin, a plant flavone from Scutellariae radix, attenuated ovalbumin-induced airway inflammation in mouse model of asthma via the suppression of IL-4/STAT6 signaling.
abstract: Bronchial asthma is a chronic inflammatory disease of the airways characterized by a marked infiltration of eosinophils at the site of inflammation. Eotaxins are potent chemoattractants for eosinophils and play important roles in pathogenesis of asthma. In the course of screening for eotaxin-3 inhibitors, we found that wogonin showed potent inhibitory activity of interleukin-4 (IL-4)-induced eotaxin-3 expression in BEAS-2B cells. In this study, we examined the effects of wogonin on IL-4/STAT6 signaling pathway and biological implication in a mouse model of asthma. Wogonin inhibited IL-4-induced activation and nuclear translocation of STAT6 which plays a key role in either the transcription of STAT6-response genes or Th2 cytokine-mediated inflammation. Oral administration of wogonin significantly reduced activation of STAT6 in the lung and the expression of eotaxin and RANTES in bronchoalveolar lavage fluids. Histological examination of lung tissue demonstrated that wogonin significantly inhibited allergen-induced eosinophilic inflammation. Administration of wogonin reduced the total IgE and ovalbumin-specific IgE levels compared with the ovalbumin-challenged group. All of these data demonstrated that wogonin could alleviate airway inflammation through inhibition of STAT6 activation induced by Th2 cytokines. Our finding implicates a potential therapeutic value of wogonin in the treatment of asthma through regulation of IL-4/STAT6 signaling pathway.
authors.affiliation: Laboratory of Chemoprevention, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-840, Korea.
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Ryu
authors.affiliation: Laboratory of Chemoprevention, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-840, Korea.
authors.forename: Tae-Hyun
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Laboratory of Chemoprevention, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-840, Korea.
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Jang
authors.affiliation: Department of Otolaryngology, Gil Medical Center, School of Medicine, Gachon University, Incheon 405-760, Korea.
authors.forename: Yoon Suk
authors.initials: YS
authors.lastname: Choi
authors.affiliation: Department of Otolaryngology, Gil Medical Center, School of Medicine, Gachon University, Incheon 405-760, Korea.
authors.forename: Seon Tae
authors.initials: ST
authors.lastname: Kim
authors.affiliation: CHA University Cancer Prevention Research Center, CHA Bio Complex, 335 Pangyo-ro, Bundang-gu, Seongnam 463-400, Korea.
authors.forename: Ki Baik
authors.initials: KB
authors.lastname: Hahm
authors.affiliation: Laboratory of Chemoprevention, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-840, Korea.
authors.forename: Ho-Jae
authors.initials: HJ
authors.lastname: Lee
keywords: STAT6; airway inflammation; bronchial asthma; eotaxin-3; wogonin