title: No difference in CCND1 gene expression between breast cancer patients with and without lymph node metastasis in a Southern Brazilian sample.
abstract: The Cyclin D1 protein has been extensively studied over the last decades, for its various roles in physiological processes, both in normal and cancer cells. Gene amplifications and overexpression of CCND1 are frequently reported in several types of cancers, including breast carcinomas, showing the increasing relevance of Cyclin D1 in tumorigenesis. Little is known about the role of this protein in the metastatic process, and the main objective of this study was to evaluate the importance of the CCND1 as a potential marker of tumor progression in breast carcinomas, in a sample collected in Southern Brazil. We studied 41 samples of formalin-fixed paraffin-embedded tissue sections from invasive ductal breast carcinomas subdivided into metastatic (n = 19) and non-metastatic (n = 22) tumors. Gene expression analysis was performed through Quantitative Real-Time PCR and immunohistochemistry. In spite of the higher expression levels of CCND1 mRNA and protein in tumors when compared with the control samples, no differences were observed between the metastatic and non-metastatic groups, suggesting that, in these samples, the expression of CCND1 has no significant influence on the metastatic process. Further studies must be performed in an attempt to clarify the diagnostic and prognostic value of Cyclin D1 in breast cancers, as well as the mechanisms that trigger its overexpression in tumors.
authors.affiliation: Departamento de Genética, Universidade Federal do Paraná, Centro Politécnico, Jardim das Américas, Curitiba, Paraná, Brazil.
authors.forename: L B L
authors.initials: LB
authors.lastname: Maia
authors.affiliation: Citolab- Laboratório de Citopatologia e Histopatologia, Batel, Curitiba, Paraná, Brazil.
authors.forename: F S C
authors.initials: FS
authors.lastname: Breginski
authors.affiliation: Citolab- Laboratório de Citopatologia e Histopatologia, Batel, Curitiba, Paraná, Brazil.
authors.forename: T C S
authors.initials: TC
authors.lastname: Cavalcanti
authors.affiliation: Departamento de Genética, Universidade Federal do Paraná, Centro Politécnico, Jardim das Américas, Curitiba, Paraná, Brazil.
authors.forename: R L R
authors.initials: RL
authors.lastname: de Souza
authors.affiliation: Departamento de Genética, Universidade Federal do Paraná, Centro Politécnico, Jardim das Américas, Curitiba, Paraná, Brazil.
authors.forename: V M S
authors.initials: VM
authors.lastname: Roxo
authors.affiliation: Departamento de Genética, Universidade Federal do Paraná, Centro Politécnico, Jardim das Américas, Curitiba, Paraná, Brazil. eribeiro@ufpr.br.
authors.forename: E M S F
authors.initials: EM
authors.lastname: Ribeiro
mesh_terms: D000328:Adult; D000368:Aged; D001938:Brazil; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D019938:Cyclin D1 / Q000235:genetics* / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D011379:Prognosis
chemical_list: C530104:CCND1 protein, human; D019938:Cyclin D1
keywords: Breast cancer; CCND1; Gene expression; Metastasis