title: Sequence-specific solid-state NMR assignments of the mouse ASC PYRIN domain in its filament form.
abstract: The apoptosis-associated speck-like protein (ASC protein) plays a central role in eukaryotic innate immune response. Upon infection, multiple ASC molecules assemble into long filaments, which are fundamental for triggering the cellular defense mechanism by starting an inflammatory cascade with the activation of caspase-1. ASC is composed of two domains, the C-terminal caspase-recruitment domain, which is involved in the recruitment of the caspase, and the N-terminal PYRIN domain (PYD), which is responsible for the formation of the filament. Here we present the (13)C and (15)N chemical shift assignment for filaments formed by the PYD of mouse ASC, a 91-residue protein. The backbone between residues 4 and 84 is assigned without interruption. Also, 86 % of the sidechain resonances for this stretch are assigned. Residues 1-3 and 85-91 show unfavorable dynamics and are not observed. Secondary chemical-shift analysis shows the presence of six α-helices.
authors.affiliation: Physical Chemistry, ETH Zurich, Vladimir-Prelog-Weg 2, 8093, Zurich, Switzerland.
authors.forename: Francesco
authors.initials: F
authors.lastname: Ravotti
authors.affiliation: Biozentrum, University of Basel, Klingelbergstrasse 70, 4056, Basel, Switzerland.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Sborgi
authors.affiliation: Physical Chemistry, ETH Zurich, Vladimir-Prelog-Weg 2, 8093, Zurich, Switzerland.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Cadalbert
authors.affiliation: Physical Chemistry, ETH Zurich, Vladimir-Prelog-Weg 2, 8093, Zurich, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Huber
authors.affiliation: Biozentrum, University of Basel, Klingelbergstrasse 70, 4056, Basel, Switzerland.|Research IT, Biozentrum, University of Basel, Klingelbergstrasse 70, 4056, Basel, Switzerland.
authors.forename: Adam
authors.initials: A
authors.lastname: Mazur
authors.affiliation: Biozentrum, University of Basel, Klingelbergstrasse 70, 4056, Basel, Switzerland.
authors.forename: Petr
authors.initials: P
authors.lastname: Broz
authors.affiliation: Biozentrum, University of Basel, Klingelbergstrasse 70, 4056, Basel, Switzerland. sebastian.hiller@unibas.ch.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Hiller
authors.affiliation: Physical Chemistry, ETH Zurich, Vladimir-Prelog-Weg 2, 8093, Zurich, Switzerland. beme@ethz.ch.
authors.forename: Beat H
authors.initials: BH
authors.lastname: Meier
authors.affiliation: Institut de Biologie et Chimie des Protéines, Bases Moléculaires et Structurales des Systèmes Infectieux, Labex Ecofect, UMR 5086 CNRS, Université de Lyon, Lyon, France. a.bockmann@ibcp.fr.
authors.forename: Anja
authors.initials: A
authors.lastname: Böckmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry*; D053468:CARD Signaling Adaptor Proteins; D051379:Mice; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D000071196:Pyrin Domain
chemical_list: D051017:Apoptosis Regulatory Proteins; D053468:CARD Signaling Adaptor Proteins; C498534:Pycard protein, mouse
keywords: Filament; Mouse ASC PYRIN domain; Sequential assignments; Solid-state NMR