title: Sensitive and simultaneous quantification of zinc pyrithione and climbazole deposition from anti-dandruff shampoos onto human scalp.
abstract: A sensitive ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method has been developed and validated for simultaneous quantification of zinc pyrithione (ZPT) and climbazole (CBZ) deposited onto human scalp from anti-dandruff (AD) shampoos. Scrubbing with a buffer solution was used as the sampling method for the extraction of ZPT and CBZ from scalp. Derivatization of ZPT was carried out prior to UHPLC-MS/MS analysis. The identification of ZPT and CBZ was performed by examining ratios of selected multiple reaction monitoring (MRM) transitions in combination with UHPLC retention times. The limit of detection for ZPT and CBZ was established to be 1 and 2ng/mL, respectively. This sensitivity enables the quantification of ZPT and CBZ at deposition levels in the low ng/cm(2) range. The method was successfully applied for the analysis of scalp buffer scrub samples from an in vivo study. The levels of ZPT and CBZ deposited on the scalp at different time points after application of the AD shampoo were measured. The results revealed that dual-active AD shampoo delivered more ZPT onto the scalp in a single wash than single active shampoo did. The amount of ZPT and CBZ retained on the scalp after AD shampoo application declined over 72h.
authors.affiliation: Unilever Research & Development Shanghai, 66 Lin Xin Road, Linkong Economic Development Zone, Shanghai 200335, People's Republic of China; Analytical-Chemistry Group, Van't Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands. Electronic address: leon.chen@unilever.com.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Unilever Research & Development Shanghai, 66 Lin Xin Road, Linkong Economic Development Zone, Shanghai 200335, People's Republic of China.
authors.forename: Miao
authors.initials: M
authors.lastname: Miao
authors.affiliation: Unilever Research & Development Shanghai, 66 Lin Xin Road, Linkong Economic Development Zone, Shanghai 200335, People's Republic of China.
authors.forename: Michael
authors.initials: M
authors.lastname: Hoptroff
authors.affiliation: Unilever Research & Development Shanghai, 66 Lin Xin Road, Linkong Economic Development Zone, Shanghai 200335, People's Republic of China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Fei
authors.affiliation: Unilever Research & Development Port Sunlight, Quarry Road East, Bebington Merseyside CH63 3JW, UK.
authors.forename: Luisa Z
authors.initials: LZ
authors.lastname: Collins
authors.affiliation: Unilever Research & Development Port Sunlight, Quarry Road East, Bebington Merseyside CH63 3JW, UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Jones
authors.affiliation: Analytical-Chemistry Group, Van't Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands; Unilever Research and Development Vlaardingen, P.O. Box 114, 3130 AC Vlaardingen, The Netherlands.
authors.forename: Hans-Gerd
authors.initials: HG
authors.lastname: Janssen
mesh_terms: D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D063807:Dandruff / Q000188:drug therapy*; D005260:Female; D006203:Hair Preparations / Q000737:chemistry*; D006801:Humans; D007093:Imidazoles / Q000032:analysis* / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D009942:Organometallic Compounds / Q000032:analysis* / Q000627:therapeutic use; D011725:Pyridines / Q000032:analysis* / Q000627:therapeutic use; D012535:Scalp / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D055815:Young Adult
chemical_list: D006203:Hair Preparations; D007093:Imidazoles; D009942:Organometallic Compounds; D011725:Pyridines; C108286:climbazole; C010423:pyrithione zinc
keywords: Anti-dandruff shampoo; Climbazole; Deposition; Scalp; UHPLC–MS/MS; Zinc pyrithione