title: Extracts of Cordia gilletii de wild (Boraginaceae) quench the quorum sensing of Pseudomonas aeruginosa PAO1.
abstract: OBJECTIVE
  The fight against infectious diseases and antimicrobial resistances needs the exploration of new active compounds with new proprieties like disrupting quorum sensing (QS) mechanisms, which is a cell-to-cell communication that regulates bacterial virulence factors. In this work, leaves and root barks extracts of a Congolese medicinal plant, Cordia gilletii, were investigated for their effect on the production of Pseudomonas aeruginosa major virulence factors regulated by QS.
  
  
  METHODS
  The effect of C. gilletii extracts on virulence factors of P. aeruginosa PAO1 was studied by the evaluation of the production of pyocyanine, elastase and biofilm; and by the measurement of the expression of QS-related genes.
  
  
  RESULTS
  The dichloromethane extract from root barks was found to quench the production of pyocyanin, a QS-dependent virulence factor in P. aeruginosa PAO1. Moreover, this extract specifically inhibits the expression of several QS-regulated genes (i.e. lasB, rhlA, lasI, lasR, rhlI, and rhlR) and reduces biofilm formation by PAO1.
  
  
  CONCLUSIONS
  This study contributes to explain the efficacy of C. gilletii in the traditional treatment of infectious diseases caused by P. aeruginosa.
authors.affiliation: Free University of Brussels, Laboratory of Pharmacognosy, Bromatology and Human Nutrition, Bld du Triomphe, CP 205/09, 1050 Brussels, Belgium ; University of Mons, Laboratoty of Therapeutic Chemistry ad Pharmacognosy, 20 Place du Parc, 7000 Mons, Belgium.
authors.forename: Philippe N
authors.initials: PN
authors.lastname: Okusa
authors.affiliation: Free University of Brussels, Laboratory of Vegetal Biotechnology, 12 Rue des Professeurs Jenner et Brachet, 6041 Gosselies, Belgium.
authors.forename: Tsiry
authors.initials: T
authors.lastname: Rasamiravaka
authors.affiliation: Free University of Brussels, Laboratory of Vegetal Biotechnology, 12 Rue des Professeurs Jenner et Brachet, 6041 Gosselies, Belgium.
authors.forename: Olivier
authors.initials: O
authors.lastname: Vandeputte
authors.affiliation: Free University of Brussels, Laboratory of Pharmacognosy, Bromatology and Human Nutrition, Bld du Triomphe, CP 205/09, 1050 Brussels, Belgium.
authors.forename: Caroline
authors.initials: C
authors.lastname: Stévigny
authors.affiliation: Free University of Brussels, Laboratory of Vegetal Biotechnology, 12 Rue des Professeurs Jenner et Brachet, 6041 Gosselies, Belgium.
authors.forename: Mondher El
authors.initials: ME
authors.lastname: Jaziri
authors.affiliation: Free University of Brussels, Laboratory of Pharmacognosy, Bromatology and Human Nutrition, Bld du Triomphe, CP 205/09, 1050 Brussels, Belgium ; University of Mons, Laboratoty of Therapeutic Chemistry ad Pharmacognosy, 20 Place du Parc, 7000 Mons, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Duez
keywords: Antimicrobial; Cordia gilletii; quorum-sensing