title: A Validated, Rapid HPLC-ESI-MS/MS Method for the Determination of Lycopsamine.
abstract: The aim of the present work was to develop and validate an HPLC-MS/MS method for the determination of a major pyrrolizidine alkaloid of comfrey (lycopsamine) in aqueous samples as a basis for the development of a method for the determination of absorption of lycopsamine by human skin. A linear calibration curve was established in the range of 1.32-440 ng. The intraday precision during the 3-day validation period ranged between 0.57 and 2.48% while the interday precision was 1.70% and 1.95% for quality control samples. LOD was 0.014 ng and recovery was above 97%. The lycopsamine content of the samples stored for 9 and 25 days at 22 degrees C, 10 degrees C and -25 degrees C did not vary. These results underline the good repeatability and accuracy of our method and allow the analysis of samples with very low lycopsamine content.
authors.forename: Nikoletta
authors.initials: N
authors.lastname: Jedlinszki
authors.forename: Dezső
authors.initials: D
authors.lastname: Csupor
mesh_terms: D019342:Acetic Acid; D002851:Chromatography, High Pressure Liquid; D020844:Comfrey / Q000737:chemistry*; D011763:Pyrrolizidine Alkaloids / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D011763:Pyrrolizidine Alkaloids; D019342:Acetic Acid; C018336:indicine