title: Detection of metabolites discriminating subtypes of thyroid cancer: Molecular profiling of FFPE samples using the GC/MS approach.
abstract: One of the critical issues in thyroid cancer diagnostic is differentiation between follicular adenoma, follicular carcinoma and the follicular variant of papillary carcinoma, which in some cases is not possible based on histopathological features only. In this paper we performed molecular profiling of thyroid tissue aiming to identify metabolites characteristic for different types of thyroid cancer. FFPE tissue specimens were analysed from 5 different types of thyroid malignancies (follicular, papillary/classical variant, papillary/follicular variant, medullary and anaplastic cancers), benign follicular adenoma and normal thyroid. Extracted metabolites were identified and semi-quantified using the GC/MS approach. There were 28 metabolites identified, whose abundances were significantly different among different types of thyroid tumours, including lipids, carboxylic acids, and saccharides. We concluded, that multi-component metabolome signature could be used for classification of different subtypes of follicular thyroid lesions. Moreover, potential applicability of the GC/MS-based analysis of FFPE tissue samples in diagnostics of thyroid cancer has been proved.
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-101 Gliwice, Poland. Electronic address: awojakowska@io.gliwice.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Wojakowska
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-101 Gliwice, Poland. Electronic address: mchekan@io.gliwice.pl.
authors.forename: Mykola
authors.initials: M
authors.lastname: Chekan
authors.affiliation: Institute of Bioorganic Chemistry Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland. Electronic address: lukasmar@ibch.poznan.pl.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Marczak
authors.affiliation: Warwick Systems Biology Centre, University of Warwick, CV4 7AL, UK. Electronic address: k.t.polanski@warwick.ac.uk.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Polanski
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-101 Gliwice, Poland. Electronic address: dlange@io.gliwice.pl.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Lange
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-101 Gliwice, Poland. Electronic address: m_pietrowska@io.gliwice.pl.
authors.forename: Monika
authors.initials: M
authors.lastname: Pietrowska
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-101 Gliwice, Poland. Electronic address: piotr.widlak@io.gliwice.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Widlak
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000175:diagnosis* / Q000378:metabolism; D002264:Carboxylic Acids / Q000032:analysis; D003937:Diagnosis, Differential; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008055:Lipids / Q000032:analysis; D055442:Metabolome; D011134:Polysaccharides / Q000032:analysis; D012680:Sensitivity and Specificity; D013964:Thyroid Neoplasms / Q000175:diagnosis* / Q000378:metabolism
chemical_list: D002264:Carboxylic Acids; D008055:Lipids; D011134:Polysaccharides
keywords: Clinical metabolomics; Follicular lesions; Formalin-fixed paraffin-embedded tissue specimen; Gas chromatography/mass spectrometry; Thyroid cancer