title: Specific Adsorption via Peptide Tags: Oriented Grafting and Release of Growth Factors for Tissue Engineering.
abstract: Numerous strategies have been proposed to decorate biomaterials with growth factors (GFs) for tissue engineering applications; their practicability as clinical tools, however, remains uncertain. We previously presented two complementary amphipathic peptides, namely, E5 and K5, which could be utilized as tags to direct GF capture onto organic materials via E5/K5 coiled-coil interactions. We here investigated their potential as mediators of GF physical adsorption. Enzyme-linked immunosorbent assays highlighted that both electrostatic and hydrophobic interactions could contribute to the adsorption process, without interfering with the peptides propensity for coiled-coil interactions. E5-tagged vascular endothelial growth factor, in particular, was efficiently adsorbed to poly(allylamine)-functionalized polystyrene, was maintained in a bioactive state and was steadily liberated over several days with little initial burst. This simple immobilization procedure was successfully applied to poly(ethylene terephthalate) films. Altogether, our data demonstrated that coil-tag-directed adsorption is a tunable, versatile and straightforward strategy to decorate biomaterials with GFs.
authors.affiliation: Department of Chemical Engineering and ‡Institute of Biomedical Engineering, Groupe de Recherche en Sciences et Technologies Biomédicales (GRSTB), Bio-P2 Research Unit, École Polytechnique de Montréal , P.O. Box 6079, succ. Centre-Ville, Montréal, Quebec, Canada H3C 3A7.
authors.forename: Frederic
authors.initials: F
authors.lastname: Murschel
authors.affiliation: Department of Chemical Engineering and ‡Institute of Biomedical Engineering, Groupe de Recherche en Sciences et Technologies Biomédicales (GRSTB), Bio-P2 Research Unit, École Polytechnique de Montréal , P.O. Box 6079, succ. Centre-Ville, Montréal, Quebec, Canada H3C 3A7.
authors.forename: Aldo
authors.initials: A
authors.lastname: Zaimi
authors.affiliation: Department of Chemical Engineering and ‡Institute of Biomedical Engineering, Groupe de Recherche en Sciences et Technologies Biomédicales (GRSTB), Bio-P2 Research Unit, École Polytechnique de Montréal , P.O. Box 6079, succ. Centre-Ville, Montréal, Quebec, Canada H3C 3A7.
authors.forename: Samantha
authors.initials: S
authors.lastname: Noel
authors.affiliation: Department of Chemical Engineering and ‡Institute of Biomedical Engineering, Groupe de Recherche en Sciences et Technologies Biomédicales (GRSTB), Bio-P2 Research Unit, École Polytechnique de Montréal , P.O. Box 6079, succ. Centre-Ville, Montréal, Quebec, Canada H3C 3A7.
authors.forename: Mario
authors.initials: M
authors.lastname: Jolicoeur
authors.affiliation: Department of Chemical Engineering and ‡Institute of Biomedical Engineering, Groupe de Recherche en Sciences et Technologies Biomédicales (GRSTB), Bio-P2 Research Unit, École Polytechnique de Montréal , P.O. Box 6079, succ. Centre-Ville, Montréal, Quebec, Canada H3C 3A7.
authors.forename: Gregory
authors.initials: G
authors.lastname: De Crescenzo
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D055767:Immobilized Proteins / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry; D011093:Polyethylene Terephthalates / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D055767:Immobilized Proteins; D010455:Peptides; D011073:Polyamines; D011093:Polyethylene Terephthalates; D042461:Vascular Endothelial Growth Factor A; C063994:polyallylamine