title: Effect of Camphylobacter rectus on Serum Iron and Transferrin- In-Vivo Findings.
abstract: OBJECTIVE
  Periodontopathogens require iron constituents for their growth and metabolism in subgingival crevice. In this study, C.rectus was detected and quantified by using 16s rDNA based PCR in chronic periodontitis and compared with the levels of serum iron, total iron binding capacity and transferrin in chronic periodontitis and healthy sites.
  
  
  METHODS
  One hundred twenty subjects divided into chronic periodontitis and healthy controls. Deep subgingival plaque was collected and genomic DNA was extracted from each sample analysed for C.rectus using 16s rRNA based PCR analysis. Blood samples were collected from both groups for estimation of serum iron, serum total iron binding capacity and serum transferrin levels. The quantified bacterial count was compared with blood samples. C. rectus was detected in both groups.
  
  
  RESULTS
  There was significant increase in bacterial count in chronic periodontitis (p<0.05). Serum iron level was significantly raised in healthy group. TIBC and transferrin levels were elevated in periodontitis. Although these differences were non-significant. Regression analysis showed significant linear relationship between C.rectus counts and decreasing iron levels and consequently increasing serum transferrin and TIBC (p<0.05).
  
  
  CONCLUSIONS
  The preliminary in vivo findings suggests C.rectus requires iron as a significant source of nutrition for its survival and growth form its hosts in deeper subgingival sites.
authors.affiliation: Reader, Department of Periodontology, Sinhgad Dental College and Hospital , Off sinhgad Road, Pune, Maharashtra, India .
authors.forename: Raghavendra Shrishail
authors.initials: RS
authors.lastname: Medikeri
authors.affiliation: Professor and Head, Department of Periodontology, Sinhgad Dental College and Hospital , Off sinhgad Road, Pune, Maharashtra, India .
authors.forename: Suresh Vasant
authors.initials: SV
authors.lastname: Lele
authors.affiliation: Research Student, Department of Periodontology, Sinhgad Dental College and Hospital , Off sinhgad Road, Pune, Maharashtra, India .
authors.forename: Pradnya Prabhakar
authors.initials: PP
authors.lastname: Mali
authors.affiliation: Research Student, Department of Periodontology, Sinhgad Dental College and Hospital , Off sinhgad Road, Pune, Maharashtra, India .
authors.forename: Pinal Mahendra
authors.initials: PM
authors.lastname: Jain
authors.affiliation: Professor, Department of Oral and Maxillofacial Surgery, Rangta College of Dental Sciences and Research , Bhilai, Chattisgarh, India .
authors.forename: Dattatray Anant
authors.initials: DA
authors.lastname: Darawade
authors.affiliation: Reader, Department of Oral Medicine and Radiology, Sinhgad Dental College and Hospital , Off sinhgad Road, Pune, Maharashtra, India .
authors.forename: Manjushri Raghavendra
authors.initials: MR
authors.lastname: Medikeri
keywords: C.rectus; Chronic periodontitis; Microbiology/polymerase chain reaction