title: Peptide Recognition of Cholesterol in Fluid Phospholipid Bilayers.
abstract: N-Acetyl-LWYIKC-amide, a cholesterol recognition/interaction amino acid consensus (CRAC) peptide, has been found capable of recognizing an exchangeable form of cholesterol in liquid-disordered (l(d)) bilayers derived from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). In sharp contrast, such recognition is barely detectable in analogous cholesterol-rich, liquid-ordered (l0) bilayers. These findings represent the first evidence for a peptide favoring a sterol as a nearest-neighbor in fluid bilayers. They also reveal that such recognition can be strongly dependent on the degree of compactness of the membrane.
authors.affiliation: Department of Chemistry, Lehigh University , Bethlehem, Pennsylvania 18015, United States.
authors.forename: Masaru
authors.initials: M
authors.lastname: Mukai
authors.affiliation: Department of Chemistry, Lehigh University , Bethlehem, Pennsylvania 18015, United States.
authors.forename: Martin R
authors.initials: MR
authors.lastname: Krause
authors.affiliation: Department of Chemistry, Lehigh University , Bethlehem, Pennsylvania 18015, United States.
authors.forename: Steven L
authors.initials: SL
authors.lastname: Regen
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D010455:Peptides; D010743:Phospholipids; D015060:1,2-Dipalmitoylphosphatidylcholine; C081581:colfosceril palmitate; D002784:Cholesterol