title: Estimating the Amount of Hemoglobin in the Neuroretinal Rim Using Color Images and OCT.
abstract: To calculate the amount of hemoglobin (Hb) in the optic nerve head (ONH), using superimposed color fundus images with disc, rim and cup boundaries obtained by OCT-Cirrus.
  
  
  
  We examined 100 healthy and 121 glaucomatous eyes using Oculus-Spark perimetry, Cirrus-OCT and Visucam (Zeiss) ONH color images. The Laguna ONhE program was then used to calculate the amount of Hb in the cup and six sectors of the rim. Receiver operating characteristic (ROC) analysis was performed and correlations between parameters were calculated.
  
  
  
  In suspected and confirmed glaucoma, Hb was significantly lower than controls in all rim sectors, especially the inferior and superonasal (p < 0.0001). Mean deviation (MD) of visual field regions showed greater correlation with the amount of Hb in the superior and inferior sectors of the rim than with rim area (p = 0.02) or nerve fiber layer thickness (p < 0.0001). On ROC analysis, the best diagnostic indicators were OCT rim area, vertical cup/disc ratio (C/D) and Glaucoma Discriminant Function (GDF) of Laguna ONhE, without significant differences.
  
  
  
  The amount of Hb in the ONH seems to have an important relationship with glaucomatous visual field sensitivity. The remaining rim has insufficient perfusion in many cases of glaucoma.
authors.affiliation: a Hospital Universitario de Canarias, University of La Laguna , Santa Cruz de Tenerife , Spain and.
authors.forename: Erica
authors.initials: E
authors.lastname: Medina-Mesa
authors.affiliation: a Hospital Universitario de Canarias, University of La Laguna , Santa Cruz de Tenerife , Spain and.
authors.forename: Marta
authors.initials: M
authors.lastname: Gonzalez-Hernandez
authors.affiliation: b Department of Computer Science , University of La Laguna , Santa Cruz de Tenerife , Spain.
authors.forename: Jose
authors.initials: J
authors.lastname: Sigut
authors.affiliation: b Department of Computer Science , University of La Laguna , Santa Cruz de Tenerife , Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Fumero-Batista
authors.affiliation: a Hospital Universitario de Canarias, University of La Laguna , Santa Cruz de Tenerife , Spain and.
authors.forename: Cristina
authors.initials: C
authors.lastname: Pena-Betancor
authors.affiliation: b Department of Computer Science , University of La Laguna , Santa Cruz de Tenerife , Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Alayon
authors.affiliation: a Hospital Universitario de Canarias, University of La Laguna , Santa Cruz de Tenerife , Spain and.
authors.forename: Manuel
authors.initials: M
authors.lastname: Gonzalez de la Rosa
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D005901:Glaucoma / Q000175:diagnosis / Q000378:metabolism* / Q000503:physiopathology; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009898:Optic Disk / Q000378:metabolism* / Q000473:pathology; D011446:Prospective Studies; D012165:Retinal Ganglion Cells / Q000378:metabolism* / Q000473:pathology; D041623:Tomography, Optical Coherence / Q000379:methods*; D014794:Visual Fields / Q000502:physiology*
chemical_list: D015415:Biomarkers; D006454:Hemoglobins
keywords: Glaucoma; hemoglobin; optic nerve head; perfusion; perimetry; visual field