title: Bio-coordination of bismuth in Helicobacter pylori revealed by immobilized metal affinity chromatography.
abstract: Over 300 Bi-binding peptides from 166 proteins in H. pylori were identified by Bi-IMAC. Bi(3+) exhibits high selectivity towards peptide enriched by cysteines and histidines with dominated motif patterns of CXnC, CXnH and HXnH. Structural rationalization and functional categorization on the identified Bi-binding peptides and proteins provide an insight into the inhibitory action of bismuth drugs.
authors.affiliation: Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China. hsun@hku.hk.
authors.forename: Yuchuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China. hsun@hku.hk.
authors.forename: Cheuk-Nam
authors.initials: CN
authors.lastname: Tsang
authors.affiliation: Centre for Genomic Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China. hsun@hku.hk.
authors.forename: Pak-Wing
authors.initials: PW
authors.lastname: Kong
authors.affiliation: Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China. hsun@hku.hk.
authors.forename: Ligang
authors.initials: L
authors.lastname: Hu
authors.affiliation: Centre for Genomic Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China.
authors.forename: Junwen
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China. hsun@hku.hk.
authors.forename: Ivan Keung
authors.initials: IK
authors.lastname: Chu
authors.affiliation: Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China. hsun@hku.hk.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China. hsun@hku.hk.
authors.forename: Hongzhe
authors.initials: H
authors.lastname: Sun
mesh_terms: D001729:Bismuth / Q000737:chemistry / Q000378:metabolism*; D002846:Chromatography, Affinity; D056831:Coordination Complexes / Q000737:chemistry / Q000378:metabolism; D016503:Drug Delivery Systems; D016480:Helicobacter pylori / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary
chemical_list: D056831:Coordination Complexes; D010455:Peptides; D001729:Bismuth