title: A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS.
abstract: Commercial extraction methods for identification of mycobacteria using MALDI-ToF MS are laborious and time consuming. We have developed a novel extraction method which utilizes a bead beater and zirconia/silica beads to significantly shorten the existing protocol. This novel method provides a more rapid extraction of mycobacteria versus the commercial standard.
authors.affiliation: The Johns Hopkins Medical Institutions. Electronic address: latonziaadams@gmail.com.
authors.forename: La'Tonzia L
authors.initials: LL
authors.lastname: Adams
authors.affiliation: The Johns Hopkins Medical Institutions; Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Parichat
authors.initials: P
authors.lastname: Salee
authors.affiliation: The Johns Hopkins Medical Institutions.
authors.forename: Kim
authors.initials: K
authors.lastname: Dionne
authors.affiliation: The Johns Hopkins Medical Institutions.
authors.forename: Karen
authors.initials: K
authors.lastname: Carroll
authors.affiliation: The Johns Hopkins Medical Institutions.
authors.forename: Nicole
authors.initials: N
authors.lastname: Parrish
mesh_terms: D006801:Humans; D009161:Mycobacterium / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000382:microbiology*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
keywords: MALDI-ToF MS; Mycobacterium; Protein extraction