title: [MiR-211 promotes invasion of hepatoma cells by targeting estrogen receptor alpha].
abstract: OBJECTIVE
  To investigate the regulatory functions and molecular mechanisms of miR211 in hepatocellular carcinoma (HCC).
  
  
  METHODS
  Real-time reverse transcription-PCR was used to analyze the expression of miR-211 in 20 paired clinical specimens of HCC and adjacent noncancerous tissues.QGY7703 and HepG2 cells with stimulation or inhibition of miR-211 expression were used to evaluate the effects on malignant phenotypes with the transwell invasion assay. Candidate target genes of miR-211 were identified by bioinformatic screening and verified by the EGFP report assay, real-time PCR and western blotting. Moreover, the regulatory functions of miR-211 on the target genes were investigated by RNA interference and cell phenotype assays.
  
  
  RESULTS
  miR-211 was up-regulated in HCC tissue specimens (t =6.26, P < 0.01).HCC cells overexpressing miR-211 showed greater invasive capacity than cells with inhibited expression (QGY-7703:t =12.59, P < 0.01; HepG2: t =17.82, P < 0.01). Estrogen receptor a (ESR1) was identified and validated as a target gene of miR-211; knockdown of ESR 1 promoted HCC invasive capacity (QGY-7703:t =8.97, P < 0.01; HepG2:t =29.31, P < 0.01).
  
  
  CONCLUSIONS
  miR-211 promotes invasion of carcinoma cells by directly targeting ESR1.
authors.affiliation: Department of clinical laboratory, Huanhu Hospital of Tianjin, Tianjin 300060, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Kan
authors.forename: Mengyuan
authors.initials: M
authors.lastname: Liu
mesh_terms: D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D047628:Estrogen Receptor alpha; D006801:Humans; D008113:Liver Neoplasms; D035683:MicroRNAs; D034622:RNA Interference; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D047628:Estrogen Receptor alpha; C549405:MIRN211 microRNA, human; D035683:MicroRNAs