title: A Newly Isolated Penicillium oxalicum 16 Cellulase with High Efficient Synergism and High Tolerance of Monosaccharide.
abstract: Compared to Trichoderma reesei RUT-C30 cellulase (Trcel), Penicillium oxalicum 16 cellulase (P16cel) from the fermentation supernatant produced a 2-fold higher glucose yield when degrading microcrystalline cellulose (MCC), possessed a 10-fold higher β-glucosidase (BGL) activity, but obtained somewhat lower other cellulase component activities. The optimal temperature and pH of β-1,4-endoglucanase, cellobiohydrolase, and filter paperase from P16cel were 50-60 °C and 4-5, respectively, but those of BGL reached 70 °C and 5. The cellulase cocktail of P16cel and Trcel had a high synergism when solubilizing MCC and generated 1.7-fold and 6.2-fold higher glucose yields than P16cel and Trcel at the same filter paperase loading, respectively. Additional low concentration of fructose enhanced the glucose yield during enzymatic hydrolysis of MCC; however, additional high concentration of monosaccharide (especially glucose) reduced cellulase activities and gave a stronger monosaccharide inhibition on Trcel. These results indicate that P16cel is a more excellent cellulase than Trcel.
authors.affiliation: College of Life Science, Jiangxi Normal University, Nanchang, 330022, China. xhzhao@jxnu.edu.cn.
authors.forename: Xi-Hua
authors.initials: XH
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Life Science, Jiangxi Normal University, Nanchang, 330022, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Tong
authors.affiliation: Research Center for Biomass Energy, East China University of Science and Technology, Shanghai, 200237, China.
authors.forename: Su-Ping
authors.initials: SP
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China. dzhwei@ecust.edu.cn.
authors.forename: Dong-Zhi
authors.initials: DZ
authors.lastname: Wei
mesh_terms: D002480:Cellulase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009005:Monosaccharides / Q000378:metabolism*; D010407:Penicillium / Q000201:enzymology*; D013696:Temperature
chemical_list: D009005:Monosaccharides; D002480:Cellulase
keywords: Cellulase; Glucose yield; Monosaccharide inhibition; Penicillium oxalicum 16; Synergism