title: GC-TOF-MS-based serum metabolomic investigations of naked oat bran supplementation in high-fat-diet-induced dyslipidemic rats.
abstract: The present study aimed to explore the metabolic response of oat bran consumption in dyslipidemic rats by a high-throughput metabolomics approach. Four groups of Sprague-Dawley rats were used: N group (normal chow diet), M group (dyslipidemia induced by 4-week high-fat feeding, then normal chow diet), OL group and OH group (dyslipidemia induced, then normal chow diet supplemented with 10.8% or 43.4% naked oat bran). Intervention lasted for 12weeks. Gas chromatography quadrupole time-of-flight mass spectrometry was used to identify serum metabolite profiles. Results confirmed the effects of oat bran on improving lipidemic variables and showed distinct metabolomic profiles associated with diet intervention. A number of endogenous molecules were changed by high-fat diet and normalized following supplementation of naked oat bran. Elevated levels of serum unsaturated fatty acids including arachidonic acid (Log2Fold of change=0.70, P=.02 OH vs. M group), palmitoleic acid (Log2Fold of change=1.24, P=.02 OH vs. M group) and oleic acid (Log2Fold of change=0.66, P=.04 OH vs. M group) were detected after oat bran consumption. Furthermore, consumption of oat bran was also characterized by higher levels of methionine and S-adenosylmethionine. Pathway exploration found that most of the discriminant metabolites were involved in fatty acid biosynthesis, biosynthesis and metabolism of amino acids, microbial metabolism in diverse environments and biosynthesis of plant secondary metabolites. These results point to potential biomarkers and underlying benefit of naked oat bran in the context of diet-induced dyslipidemia and offer some insights into the mechanism exploration.
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, 100191, PR China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, 100191, PR China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Jing
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, 100191, PR China; Department of Nutrition, China-Japan Friendship Hospital, Beijing, 100029, PR China.
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, 100191, PR China.
authors.forename: Zhaofeng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, 100191, PR China.
authors.forename: Qianying
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, 100191, PR China. Electronic address: liyongbmu@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000737:chemistry; D018554:Avena; D015415:Biomarkers / Q000097:blood; D001706:Biopsy; D004032:Diet; D059305:Diet, High-Fat; D004043:Dietary Fiber / Q000008:administration & dosage*; D050171:Dyslipidemias / Q000178:diet therapy / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000097:blood / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006949:Hyperlipidemias / Q000378:metabolism; D008099:Liver / Q000473:pathology; D008297:Male; D055432:Metabolomics; D008715:Methionine / Q000737:chemistry; D015999:Multivariate Analysis; D019301:Oleic Acid / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012436:S-Adenosylmethionine / Q000737:chemistry
chemical_list: D015415:Biomarkers; D004043:Dietary Fiber; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; C008757:palmitoleic acid; D016718:Arachidonic Acid; D019301:Oleic Acid; D012436:S-Adenosylmethionine; D008715:Methionine
keywords: Dyslipidemia; Gas chromatography quadrupole-time-of-flight mass spectrometry; High-fat diet; Metabolomics; Oat bran