title: [Study on Optimum Extraction Process of Water Soluble Protein from Coicis Semen].
abstract: OBJECTIVE
  To optimize the extraction process of water soluble protein from Coicis Semen.
  
  
  METHODS
  Coicis Semen was used to extract protein with cysteine hydrochloride aqueous solution in alkaline environment. The single factor test was conducted with the factors of extraction temperature, extraction pH, solid-liquid ratio and extraction time. In combination with single factor test results, a 4 factors 3 levels orthogonal experiment was designed with the factors of extraction temperature, extraction pH, solid-liquid ratio and extraction time. The effect of various factors on the results and the interactions were analyzed by range analysis, variance analysis and partial least squares regression analysis.
  
  
  RESULTS
  The optimum extraction process of water soluble protein from Coicis Semen was as follows: the extraction temperature was 40 °C , the extraction pH was 10, the solid-liquid ratio was 1:20 and the extraction time was 4 h. The interaction of temperature and solid-liquid ratio, as well as temperature and extraction time would lower protein extraction rate.
  
  
  CONCLUSIONS
  The optimized extraction process is economical, simple, reasonable and practicable.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Li-li
authors.initials: LL
authors.lastname: Gong
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.forename: Rong
authors.initials: R
authors.lastname: Rong
authors.forename: Jian-qing
authors.initials: JQ
authors.lastname: Ju
mesh_terms: D000704:Analysis of Variance; D031742:Coix / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry*; D013678:Technology, Pharmaceutical; D013696:Temperature; D013997:Time Factors; D014867:Water
chemical_list: D010936:Plant Extracts; D010940:Plant Proteins; D014867:Water