title: Magnetic two-dimensional molecularly imprinted materials for the recognition and separation of proteins.
abstract: Surface molecular imprinting for proteins is an emerging cross-field of molecular imprinting engineering and functional materials. In this contribution, we report a novel design of magnetic two-dimensional molecularly imprinted polymers (2D-MIPs) for the high recognition and separation of proteins. Bovine serum albumin-surface-imprinted polydopamines were prepared on the surfaces of the magnetic Fe3O4-graphene oxide hybrid to form magnetic 2D-MIPs for proteins. The 2D Fe3O4-graphene oxide substrate possesses a dominant surface-to-volume ratio in comparison to 3D spherical substrates with the same volume. These materials are sensitive to a magnetic field and can be easily separated using an external magnet. The binding experimental results of bovine serum albumin on magnetic 2D-MIPs and real sample analysis demonstrated the high recognition specificity, selectivity, accessibility and convenient separation of 2D-MIPs for template protein. The design and synthesis of magnetic 2D-MIPs provide a new perspective for the surface molecularly imprinted materials with potential in the recognition and separation of proteins.
authors.affiliation: MOE Key Laboratory of Space Applied Physics and Chemistry, Shaanxi Key Laboratory of Macromolecular Science and Technology, School of Science, Northwestern Polytechnical University, Xi'an, 710072, P. R. China. kongjie@nwpu.edu.cn.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Space Applied Physics and Chemistry, Shaanxi Key Laboratory of Macromolecular Science and Technology, School of Science, Northwestern Polytechnical University, Xi'an, 710072, P. R. China. kongjie@nwpu.edu.cn.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Zhao
authors.affiliation: MOE Key Laboratory of Space Applied Physics and Chemistry, Shaanxi Key Laboratory of Macromolecular Science and Technology, School of Science, Northwestern Polytechnical University, Xi'an, 710072, P. R. China. kongjie@nwpu.edu.cn.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: MOE Key Laboratory of Space Applied Physics and Chemistry, Shaanxi Key Laboratory of Macromolecular Science and Technology, School of Science, Northwestern Polytechnical University, Xi'an, 710072, P. R. China. kongjie@nwpu.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Kong
mesh_terms: D000818:Animals; D002417:Cattle; D006108:Graphite / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting; D010087:Oxides / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification*; D013499:Surface Properties
chemical_list: D007211:Indoles; D058185:Magnetite Nanoparticles; D010087:Oxides; D011108:Polymers; C568283:polydopamine; D012710:Serum Albumin, Bovine; D006108:Graphite