title: Complete genome sequence, metabolic model construction and phenotypic characterization of Geobacillus LC300, an extremely thermophilic, fast growing, xylose-utilizing bacterium.
abstract: We have isolated a new extremely thermophilic fast-growing Geobacillus strain that can efficiently utilize xylose, glucose, mannose and galactose for cell growth. When grown aerobically at 72 °C, Geobacillus LC300 has a growth rate of 2.15 h(-1) on glucose and 1.52 h(-1) on xylose (doubling time less than 30 min). The corresponding specific glucose and xylose utilization rates are 5.55 g/g/h and 5.24 g/g/h, respectively. As such, Geobacillus LC300 grows 3-times faster than E. coli on glucose and xylose, and has a specific xylose utilization rate that is 3-times higher than the best metabolically engineered organism to date. To gain more insight into the metabolism of Geobacillus LC300 its genome was sequenced using PacBio's RS II single-molecule real-time (SMRT) sequencing platform and annotated using the RAST server. Based on the genome annotation and the measured biomass composition a core metabolic network model was constructed. To further demonstrate the biotechnological potential of this organism, Geobacillus LC300 was grown to high cell-densities in a fed-batch culture, where cells maintained a high xylose utilization rate under low dissolved oxygen concentrations. All of these characteristics make Geobacillus LC300 an attractive host for future metabolic engineering and biotechnology applications.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware, 150 Academy St, Newark, DE 19716, USA.
authors.forename: Lauren T
authors.initials: LT
authors.lastname: Cordova
authors.affiliation: Department of Chemical and Biomolecular Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware, 150 Academy St, Newark, DE 19716, USA.
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Long
authors.affiliation: Department of Chemical and Biomolecular Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware, 150 Academy St, Newark, DE 19716, USA.
authors.forename: Keerthi P
authors.initials: KP
authors.lastname: Venkataramanan
authors.affiliation: Department of Chemical and Biomolecular Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware, 150 Academy St, Newark, DE 19716, USA. Electronic address: mranton@udel.edu.
authors.forename: Maciek R
authors.initials: MR
authors.lastname: Antoniewicz
mesh_terms: D061249:Batch Cell Culture Techniques; D018533:Biomass; D003470:Culture Media; D004734:Energy Metabolism / Q000235:genetics; D005285:Fermentation; D016680:Genome, Bacterial / Q000235:genetics*; D056506:Geobacillus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D060847:Metabolic Engineering / Q000379:methods; D053858:Metabolic Networks and Pathways / Q000235:genetics; D010085:Oxidative Phosphorylation; D014994:Xylose / Q000378:metabolism*
chemical_list: D003470:Culture Media; D014994:Xylose; D005947:Glucose
keywords: Genome analysis; High-cell-density fermentation; Metabolic network model; Thermophile; Xylose metabolism