title: DAZL Expression Explains Origin and Central Formation of Primordial Germ Cells in Chickens.
abstract: The timing and biological events associated with germ cell specification in chickens have not been determined yet. In this study, we report the origin of primordial germ cells (PGCs) and germ plasm dynamics through investigation of the expression of the chicken homolog of deleted in azoospermia-like (cDAZL) gene during germ cell specification. Asymmetric localization of germ plasm in the center of oocytes from preovulatory follicle stages leads to PGCs being formed in the center. During cleavage stages, DAZL expression pattern changes from a subcellular localization to a diffuse form before and after zygotic genome activation. Meanwhile, PGCs exhibit transcriptional active status during their specification. In addition, knockdown studies of cDAZL, which result in reduced proliferation, aberrant gene expression profiles, and PGC apoptosis in vitro, suggest its possible roles for PGC formation in chicken. In conclusion, DAZL expression reveals formation and initial positioning of PGCs in chickens.
authors.affiliation: 1 Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University , Seoul, Korea.|2 Department of Cell and Developmental Biology, University College London , London, United Kingdom .
authors.forename: Hyung Chul
authors.initials: HC
authors.lastname: Lee
authors.affiliation: 1 Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University , Seoul, Korea.
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: 1 Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University , Seoul, Korea.
authors.forename: Hyo Gun
authors.initials: HG
authors.lastname: Lee
authors.affiliation: 1 Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University , Seoul, Korea.
authors.forename: Jeong Mook
authors.initials: JM
authors.lastname: Lim
authors.affiliation: 3 Division of Animal Science, Faculty of Agriculture, Shinshu University , Nagano, Japan .
authors.forename: Tamao
authors.initials: T
authors.lastname: Ono
authors.affiliation: 1 Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University , Seoul, Korea.|4 Institute for Biomedical Sciences, Shinshu University , Nagano, Japan .
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Han
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D019521:Body Patterning / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics*; D002642:Chick Embryo; D002645:Chickens; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000166:cytology* / Q000502:physiology; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D016601:RNA-Binding Proteins / Q000502:physiology*; D015053:Zygote / Q000166:cytology / Q000502:physiology
chemical_list: D016601:RNA-Binding Proteins