title: Effects of acute handling stress on cerebral monoaminergic neurotransmitters in juvenile Senegalese sole Solea senegalensis.
abstract: Juvenile Senegalese sole Solea senegalensis were subjected for short periods to two different types of handling-related stress: air exposure stress and net handling stress. The S. senegalensis were sacrificed 2 and 24 h after the stress events and the levels of serotonin (5-HT), noradrenaline (NA), dopamine (DA) and their respective major metabolites, 5-hydroxyindoleacetic acid (5-HIAA), 3-methoxy-4-hydroxyphenylglycol (MHPG) and 3,4-dihydroxyphenylacetic acid (DOPAC), were measured in three brain regions (telencephalon, hypothalamus and optic tectum) and compared with those in control, non-stressed S. senegalensis. Neither type of stress caused any significant alteration of serotoninergic activity (5-HIAA:5-HT ratio) or NA levels. Dopaminergic activity (DOPAC:DA ratio) was lower in stressed fish in all of the brain regions studied. For both air exposure stress and net handling stress, DA levels were significantly higher (P < 0.05) than in the control S. senegalensis. In addition, the higher DA levels after net handling stress were always significantly higher (P < 0.05) than those observed after acute air exposure stress, except in the telencephalon after 24 h. The significantly lower DOPAC:DA ratio (P < 0.05) in all of the brain regions studied was only observed in response to net handling stress.
authors.affiliation: Departamento de Fisiología (Laboratorio de Fisiología Animal), Facultad de Biología, Campus Vida s/n, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.|Instituto de Acuicultura, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.|Instituto Federal Catarinense, Campus Araquari, 89245-000, Araquari, Brazil.
authors.forename: R A
authors.initials: RA
authors.lastname: Weber
authors.affiliation: Departamento de Fisiología (Laboratorio de Fisiología Animal), Facultad de Biología, Campus Vida s/n, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.|Instituto de Acuicultura, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: Pérez Maceira
authors.affiliation: Departamento de Fisiología (Laboratorio de Fisiología Animal), Facultad de Biología, Campus Vida s/n, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Aldegunde
authors.affiliation: Instituto Español de Oceanografía, Centro Oceanográfico de Vigo, Cabo Estai, Canido, 36200, Vigo, Spain.
authors.forename: J B
authors.initials: JB
authors.lastname: Peleteiro
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Campus Vida s/n, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
authors.forename: L O
authors.initials: LO
authors.lastname: García Martín
authors.affiliation: Departamento de Fisiología (Laboratorio de Fisiología Animal), Facultad de Biología, Campus Vida s/n, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.|Instituto de Acuicultura, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Aldegunde
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D005412:Flatfishes / Q000378:metabolism*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013315:Stress, Psychological / Q000378:metabolism*
chemical_list: D015306:Biogenic Monoamines; D018377:Neurotransmitter Agents; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D004298:Dopamine; D009638:Norepinephrine
keywords: dopamine; hypothalamus; noradrenaline; optic tectum; serotonin; telencephalon