title: Citreicella manganoxidans sp. nov., a novel manganese oxidizing bacterium isolated from a shallow water hydrothermal vent in Espalamaca (Azores).
abstract: A Gram-stain negative, non-motile, non-spore forming, aerobic and rod or narrow lemon-shaped bacterial strain, VSW210(T), was isolated from surface seawater in a shallow water hydrothermal vent region in Espalamaca (Azores). Strain VSW210(T) was found to grow optimally at 30 °C, at pH 7 and in the presence of 2-6 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain VSW210(T) clusters with the type strain Citreicella marina CK-I3-6(T) (sequence similarity value of 99.6 %), but DNA-DNA hybridization showed DNA-DNA relatedness between the strain VSW210(T) and C. marina CK-I3-6(T) to be 55.8 ± 3.2 %. The DNA G+C content of strain VSW210(T) was determined to be 67.4 mol%. The cellular fatty acid profiles of strain VSW210(T) was found to contain C18:1 ω7c (80.1 %) and C16:0 (9.2 %). The major polar lipids in strain VSW210(T) were identified as phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified phospholipid. Strain VSW210(T) was found to be able to oxidize soluble Mn(II) to insoluble MnO2, which was confirmed with LBB staining. Differential phenotypic properties and genetic uniqueness revealed that this strain VSW210(T) is distinguishable from other species of the genus Citreicella. On the basis of the data presented, strain VSW210(T) is considered to represent a novel species of the genus Citreicella, for which the name Citreicella manganoxidans sp. nov. is proposed. The type strain is VSW210(T) (=KCTC 32497(T) = MCC 2286(T)).
authors.affiliation: Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, Goa, 403 004, India.
authors.forename: Raju
authors.initials: R
authors.lastname: Rajasabapathy
authors.affiliation: Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, Goa, 403 004, India. cmohan@nio.org.
authors.forename: Chellandi
authors.initials: C
authors.lastname: Mohandass
authors.affiliation: NCIM Resource Center, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, 411 008, India.
authors.forename: Syed Gulam
authors.initials: SG
authors.lastname: Dastager
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People's Republic of China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.affiliation: IMAR-Department of Oceanography and Fisheries, University of Azores, Rua Prof Frederico Machado, 9901-862, Horta, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Colaço
mesh_terms: D000332:Aerobiosis; D001394:Azores; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D060086:Hydrothermal Vents / Q000382:microbiology*; D008345:Manganese / Q000378:metabolism*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D008345:Manganese; D012965:Sodium Chloride
keywords: Azores; Citreicella manganoxidans sp. nov; Hydrothermal vent; Polyphasic taxonomy; Seawater