title: Electrochemical DNA biosensor based on a glassy carbon electrode modified with gold nanoparticles and graphene for sensitive determination of Klebsiella pneumoniae carbapenemase.
abstract: We describe the fabrication of a sensitive electrochemical DNA biosensor for determination of Klebsiella pneumoniae carbapenemase (KPC). The highly sensitive and selective electrochemical biosensor for DNA detection was constructed based on a glassy carbon electrode (GCE) modified with gold nanoparticles (Au-NPs) and graphene (Gr). Then Au-NPs/Gr/GCE was characterized by scanning electro microscope (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The hybridization detection was measured by diffierential pulse voltammetry (DPV) using methylene blue (MB) as the hybridization indicator. The dynamic range of detection of the sensor for the target DNA sequences was from 1 × 10(-12) to 1 × 10(-7)mol/L, with a detection limit of 2 × 10(-13)mol/L. The DNA biosensor had excellent specificity for distinguishing complementary DNA sequence in the presence of non-complementary and mismatched DNA sequence. The results demonstrated that the Au-NPs/Gr nanocomposite was a promising substrate for the development of high-performance electrocatalysts for determination of KPC.
authors.affiliation: Public Health School, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin, Heilongjiang 150081, China.
authors.forename: Hong-zhi
authors.initials: HZ
authors.lastname: Pan
authors.affiliation: The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng Street, Harbin, Heilongjiang Province 150001, China.
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Yu
authors.affiliation: The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng Street, Harbin, Heilongjiang Province 150001, China.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng Street, Harbin, Heilongjiang Province 150001, China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng Street, Harbin, Heilongjiang Province 150001, China.
authors.forename: Guang-cai
authors.initials: GC
authors.lastname: Wan
authors.affiliation: The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng Street, Harbin, Heilongjiang Province 150001, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng Street, Harbin, Heilongjiang Province 150001, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Guan
authors.affiliation: The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng Street, Harbin, Heilongjiang Province 150001, China. Electronic address: dongchang1969@163.com.
authors.forename: Dong
authors.initials: D
authors.lastname: Chang
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics*; D053768:Metal Nanoparticles / Q000737:chemistry*; D001618:beta-Lactamases / Q000032:analysis / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D002244:Carbon; D006046:Gold; D006108:Graphite; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Determination; Electrochemical DNA biosensor; Gold nanoparticles; Graphene; Klebsiella pneumoniae carbapenemase