title: Effects of As2O3 on DNA methylation, genomic instability, and LTR retrotransposon polymorphism in Zea mays.
abstract: Arsenic is a well-known toxic substance on the living organisms. However, limited efforts have been made to study its DNA methylation, genomic instability, and long terminal repeat (LTR) retrotransposon polymorphism causing properties in different crops. In the present study, effects of As2O3 (arsenic trioxide) on LTR retrotransposon polymorphism and DNA methylation as well as DNA damage in Zea mays seedlings were investigated. The results showed that all of arsenic doses caused a decreasing genomic template stability (GTS) and an increasing Random Amplified Polymorphic DNAs (RAPDs) profile changes (DNA damage). In addition, increasing DNA methylation and LTR retrotransposon polymorphism characterized a model to explain the epigenetically changes in the gene expression were also found. The results of this experiment have clearly shown that arsenic has epigenetic effect as well as its genotoxic effect. Especially, the increasing of polymorphism of some LTR retrotransposon under arsenic stress may be a part of the defense system against the stress.
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, 25240, Erzurum, Turkey.
authors.forename: Filiz Aygun
authors.initials: FA
authors.lastname: Erturk
authors.affiliation: Department of Field Crops, Faculty of Agriculture, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Aydin
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, 25240, Erzurum, Turkey. burcu.sigmaz25@gmail.com.
authors.forename: Burcu
authors.initials: B
authors.lastname: Sigmaz
authors.affiliation: Department of Agricultural Biotechnology, Faculty of Agriculture, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: M Sinan
authors.initials: MS
authors.lastname: Taspinar
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, 25240, Erzurum, Turkey.
authors.forename: Esra
authors.initials: E
authors.lastname: Arslan
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, 25240, Erzurum, Turkey.
authors.forename: Guleray
authors.initials: G
authors.lastname: Agar
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, 25240, Erzurum, Turkey.
authors.forename: Semra
authors.initials: S
authors.lastname: Yagci
mesh_terms: D000077237:Arsenic Trioxide; D001152:Arsenicals; D019175:DNA Methylation / Q000187:drug effects; D042822:Genomic Instability / Q000187:drug effects; D010087:Oxides / Q000633:toxicity*; D011110:Polymorphism, Genetic / Q000187:drug effects; D018626:Retroelements / Q000187:drug effects*; D036226:Seedlings / Q000235:genetics; D020079:Terminal Repeat Sequences / Q000187:drug effects*; D003313:Zea mays / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism
chemical_list: D001152:Arsenicals; D010087:Oxides; D018626:Retroelements; D000077237:Arsenic Trioxide
keywords: As2O3; DNA methylation; Genomic instability; LTR retrotransposons polymorphism; Zea mays