title: Shemamruthaa, a Herbal Formulation Induces Apoptosis in Breast Cancer Cells and Inhibits Tumor Progression in Rats.
abstract: Phytochemicals present in plants are more effective than their individual constituents in preventing cancer through synergetic effects. From this perspective, Shemamruthaa, a herbal formulation was evaluated with a view to potentiate more intense anticancer property. This study investigates the anticancer activity of Shemamruthaa in breast cancer (MDA-MB 231) cell lines and its cancer therapeutic potential in 7,12-dimethylbenz[a]anthracene induced breast cancer rats. Results of MTT, trypan blue, and apoptotic marker assays suggested that Shemamruthaa can induce cytotoxicity in cancer cells, in a concentration- and time-dependent manner. Oral administration of Shemamruthaa effectively suppressed the tumor progression as evidenced by decrease in tumor volume and modulation of oxidant-antioxidant status and resulted in extended life span. Gas chromatography-mass spectrometry and high-performance liquid chromatography analysis of Shemamruthaa revealed the presence of pyrogallol, 5-hydrxoymethylfurfural, trilinolein, and flavonoids. Finally, we show that Shemamruthaa contains potential anticancer agents acting either singly or in combination against breast cancer cell proliferation.
authors.affiliation: Dr ALM Postgraduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, India Department of Medical Biochemistry, Dr. ALM P-G Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, India.
authors.forename: Ayyakkannu
authors.initials: A
authors.lastname: Purushothaman
authors.affiliation: Dr ALM Postgraduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, India Department of Medical Biochemistry, Dr. ALM P-G Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, India.
authors.forename: Elumalai
authors.initials: E
authors.lastname: Nandhakumar
authors.affiliation: Dr ALM Postgraduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, India.
authors.forename: Palanivelu
authors.initials: P
authors.lastname: Shanthi
authors.affiliation: Dr ALM Postgraduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, India Department of Medical Biochemistry, Dr. ALM P-G Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, India psachdanandam2000@yahoo.co.in.
authors.forename: Thiruvaiyaru Panchanatham
authors.initials: TP
authors.lastname: Sachidanandam
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D005260:Female; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; C000588470:shemamruthaa
keywords: Shemamruthaa; apoptosis; flavonoids; hydroxymethylfurfural; mammary carcinoma; trilinolein