title: [TISSUE-ENGINEERED SUBSTITUTION URETHROPLASTY BASED ON DECELLULARIZED VASCULAR MATRIX AND AUTOLOGOUS CELLS OF THE BUCCAL MUCOSA: THE FIRST EXPERIENCE].
abstract: Urethral strictures and anomalies remain a challenging urological problem. Reconstructive plastic surgery has been shown to be the most effective way to treat them. There are two main types of urethroplasty: anastomosis (anastomotic urethroplasty) and expansion of the urethral lumen using of flaps and grafts (substitution urethroplasty). Currently the ideal material for substitution urethroplasty does not exist. Tissue engineering of the urethra seems to be one of the most promising approaches to address this problem. Various tissues-engineering techniques were proposed for substitution urethroplasty. In this study, tissue-engineering design was based on the decellularized cadaveric arterial wall. The study results demonstrated the feasibility of creating stable tissue-engineered structures with autologous cultured epithelial cells of the buccal mucosa and decellularized matrix from human cadaveric arterial wall (DMCAW). There was a complete engraftment of tissue-engineering design based on DMCAW and buccal mucosa cells, used for substitution urethroplasty in a patient with the bulbar urethral stricture. Postoperatively (within 4 months after surgery) no complications and/or adverse events were observed. However, in the late postoperative period (12 months) there was recurrence of urethral stricture in the middle of the tissue-engineering design and the native urethra that warranted another surgery. Tissue-engineering design based on DMCAW and autologous buccal mucosa is safe as a material for substitution urethroplasty. Further research is required to ascertain the effectiveness of the method.
authors.forename: P V
authors.initials: PV
authors.lastname: Glybochko
authors.forename: Ju G
authors.initials: JG
authors.lastname: Aljaev
authors.forename: V N
authors.initials: VN
authors.lastname: Nikolenko
authors.forename: A B
authors.initials: AB
authors.lastname: Shehter
authors.forename: A Z
authors.initials: AZ
authors.lastname: Vinarov
authors.forename: L P
authors.initials: LP
authors.lastname: Istranov
authors.forename: E V
authors.initials: EV
authors.lastname: Istranova
authors.forename: R K
authors.initials: RK
authors.lastname: Abojanc
authors.forename: A V
authors.initials: AV
authors.lastname: Ljundup
authors.forename: M I
authors.initials: MI
authors.lastname: Danilevskij
authors.forename: A E
authors.initials: AE
authors.lastname: Guller
authors.forename: P A
authors.initials: PA
authors.lastname: Elistratov
authors.forename: D V
authors.initials: DV
authors.lastname: Butnaru
authors.forename: D F
authors.initials: DF
authors.lastname: Kantimerov
authors.forename: G A
authors.initials: GA
authors.lastname: Mashin
authors.forename: A S
authors.initials: AS
authors.lastname: Titov
authors.forename: A V
authors.initials: AV
authors.lastname: Proskura
authors.forename: K V
authors.initials: KV
authors.lastname: Kudrichevskaja
mesh_terms: D001158:Arteries; D001705:Bioprosthesis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology / Q000637:transplantation*; D013524:Surgical Flaps; D023822:Tissue Engineering / Q000379:methods*; D016896:Treatment Outcome; D014525:Urethral Stricture / Q000209:etiology / Q000473:pathology / Q000601:surgery*; D013521:Urologic Surgical Procedures, Male / Q000379:methods*