title: Multimode Optical Imaging for Translational Chemotherapy: In Vivo Tumor Detection and Delineation by Targeted Gallium Corroles.
abstract: We report the feasibility of tumor detection and delineationin vivousing multimode optical imaging of targeted gallium corrole (HerGa). HerGa is highly effective for targeted HER2+ tumor eliminationin vivo, and it emits intense fluorescence. These unique characteristics of HerGa prompted us to investigate the potential of HerGa for tumor detection and delineation, by performing multimode optical imagingex vivoandin vivo; the imaging modes included fluorescence intensity, spectral (including ratiometric), lifetime, and two-photon excited fluorescence, using our custom-built imaging system. While fluorescence intensity imaging provided information about tumor targeting capacity and tumor retention of HerGa, ratiometric spectral imaging offered more quantitative and specific information about HerGa location and accumulation. Most importantly, the fluorescence lifetime imaging of HerGa allowed us to discriminate between tumor and non-tumor regions by fluorescence lifetime differences. Finally, two-photon excited fluorescence images provided highly resolved and thus topologically detailed information around the tumor regions where HerGa accumulates. Taken together, the results shown in this report suggest the feasibility of tumor detection and delineation by multimode optical imaging of HerGa, and fluorescent chemotherapy agents in general. Specifically, the multimode optical imaging can offer complementary and even synergetic information simultaneously in the tumor detection and delineation by HerGa, thus enhancing contrast.
authors.affiliation: Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA ; Department of Biomedical Engineering, University of Southern California, Los Angeles, CA.
authors.forename: Jae Youn
authors.initials: JY
authors.lastname: Hwang
authors.affiliation: Beckman Research Institute, California Institute of Technology, Pasadena, CA ; Department of Chemistry, Technion-Israel Institute of Technology, Haifa.
authors.forename: Zeev
authors.initials: Z
authors.lastname: Gross
authors.affiliation: Beckman Research Institute, California Institute of Technology, Pasadena, CA.
authors.forename: Harry B
authors.initials: HB
authors.lastname: Gray
authors.affiliation: Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA ; Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA.
authors.forename: Lali K
authors.initials: LK
authors.lastname: Medina-Kauwe
authors.affiliation: Department of Biomedical Engineering, University of Southern California, Los Angeles, CA ; Spectral Molecular Imaging, Inc., Beverly Hills CA.
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Farkas
keywords: Multimode optical imaging; fluorescence lifetime imaging microscopy (FLIM); spectral imaging; tumor detection; tumor targeted corrole; two-photon excitation