title: HDAC3 Is Required for Posterior Lateral Line Development in Zebrafish.
abstract: Histone deacetylases (HDACs) are involved in multiple developmental processes, but their functions in the development of mechanosensory organs are largely unknown. In the present study, we report the presence of HDAC3 in the zebrafish posterior lateral line primordium and newly deposited neuromasts. We used morpholinos to show that HDAC3 knockdown severely disrupts the development of the posterior lateral line and reduces the numbers of neuromasts and sensory hair cells within these organs. In HDAC3 morphants, we also observed decreased cell proliferation and increased apoptosis, which might lead to these defects. Finally, we show that HDAC3 deficiency results in attenuated Fgf signaling in the migrating primordium. In situ hybridizations indicate aberrant expression patterns of Notch signaling pathway genes in HDAC3 morphants. In addition, inhibition of HDAC3 function diminishes cxcr7b and alters cxcl12a expression in the migrating primordium. Our results indicate that HDAC3 plays a crucial role in regulating posterior lateral line (PLL) formation and provide evidence for epigenetic regulation in auditory organ development.
authors.affiliation: Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, People's Republic of China.
authors.forename: Yingzi
authors.initials: Y
authors.lastname: He
authors.affiliation: Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, People's Republic of China. fjswzm2015@126.com.|Key Laboratory of Hearing Medicine of National Health and Family Planning Commission, Shanghai, People's Republic of China. fjswzm2015@126.com.
authors.forename: Zhengmin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Metabolism and Molecular Medicine, the Ministry of Education, Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai, People's Republic of China.
authors.forename: Shaoyang
authors.initials: S
authors.lastname: Sun
authors.affiliation: Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, People's Republic of China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Tang
authors.affiliation: Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, People's Republic of China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Li
authors.affiliation: Co-innovation Center of Neuroregeneration, Key Laboratory for Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, Nanjing, Jiangsu, 210096, People's Republic of China.
authors.forename: Renjie
authors.initials: R
authors.lastname: Chai
authors.affiliation: Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, People's Republic of China. hwli@shmu.edu.cn.|Key Laboratory of Hearing Medicine of National Health and Family Planning Commission, Shanghai, People's Republic of China. hwli@shmu.edu.cn.|State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, 200031, People's Republic of China. hwli@shmu.edu.cn.|Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, People's Republic of China. hwli@shmu.edu.cn.
authors.forename: Huawei
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D055785:Gene Knockdown Techniques; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000378:metabolism; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D053403:Lateral Line System / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism*; D008465:Mechanoreceptors / Q000378:metabolism; D060172:Morpholinos / Q000494:pharmacology; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D060172:Morpholinos; D012333:RNA, Messenger; D029961:Zebrafish Proteins; D016222:Fibroblast Growth Factor 2; C000625964:HDAC3 protein, zebrafish; D006655:Histone Deacetylases
keywords: Fgf signaling; HDAC3; Posterior lateral line; Zebrafish