title: Multiplexed microsatellite markers for seven Metarhizium species.
abstract: Cross-species transferability of 41 previously published simple sequence repeat (SSR) markers was assessed for 11 species of the entomopathogenic fungus Metarhizium. A collection of 65 Metarhizium strains including all 54 used in a recent phylogenetic revision of the genus were characterized. Between 15 and 34 polymorphic SSR markers produced scorable PCR amplicons in seven species, including M. anisopliae, M. brunneum, M. guizhouense, M. lepidiotae, M. majus, M. pingshaense, and M. robertsii. To provide genotyping tools for concurrent analysis of these seven species fifteen markers grouped in five multiplex pools were selected based on high allelic diversity and easy scorability of SSR chromatograms.
authors.affiliation: Molecular Ecology, Institute for Sustainability Sciences, Agroscope, Reckenholzstrasse 191, 8046 Zurich, Switzerland.
authors.forename: Johanna
authors.initials: J
authors.lastname: Mayerhofer
authors.affiliation: Molecular Ecology, Institute for Sustainability Sciences, Agroscope, Reckenholzstrasse 191, 8046 Zurich, Switzerland.
authors.forename: Andy
authors.initials: A
authors.lastname: Lutz
authors.affiliation: Molecular Ecology, Institute for Sustainability Sciences, Agroscope, Reckenholzstrasse 191, 8046 Zurich, Switzerland.
authors.forename: Franco
authors.initials: F
authors.lastname: Widmer
authors.affiliation: Systematic Mycology and Microbiology Laboratory, USDA-ARS, Beltsville, MD 20705-2350, USA.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Rehner
authors.affiliation: Plant Ecological Genetics, Institute of Integrative Biology, ETH Zurich, CH-8092 Zurich, Switzerland.
authors.forename: Adrian
authors.initials: A
authors.lastname: Leuchtmann
authors.affiliation: Molecular Ecology, Institute for Sustainability Sciences, Agroscope, Reckenholzstrasse 191, 8046 Zurich, Switzerland. Electronic address: juerg.enkerli@agroscope.admin.ch.
authors.forename: Jürg
authors.initials: J
authors.lastname: Enkerli
mesh_terms: D014644:Genetic Variation; D060005:Genotyping Techniques; D052981:Metarhizium / Q000235:genetics*; D018895:Microsatellite Repeats; D010802:Phylogeny; D017422:Sequence Analysis, DNA
keywords: Biocontrol; Cross-species amplification; Molecular genetic identification