title: Epitope Fingerprinting for Recognition of the Polyclonal Serum Autoantibodies of Alzheimer's Disease.
abstract: Autoantibodies (aAb) associated with Alzheimer's disease (AD) have not been sufficiently characterized and their exact involvement is undefined. The use of information technology and computerized analysis with phage display technology was used, in the present research, to map the epitope of putative self-antigens in AD patients. A 12-mer random peptide library, displayed on M13 phages, was screened using IgG from AD patients with two repetitions. Seventy-one peptides were isolated; however, only 10 were positive using the Elisa assay technique (Elisa Index > 1). The results showed that the epitope regions of the immunoreactive peptides, identified by phage display analysis, were on the exposed surfaces of the proteins. The putative antigens MAST1, Enah, MAO-A, X11/MINT1, HGF, SNX14, ARHGAP 11A, APC, and CENTG3, which have been associated with AD or have functions in neural tissue, may indicate possible therapeutic targets.
authors.affiliation: Laboratório de Genética, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Rua Acre s/n, Bloco 2E sala 230, Campus Umuarama, 38400-902 Uberlândia, MG, Brazil ; Faculdade de Medicina, Universidade Federal de Uberlândia, Avenida Para 1720, Bloco 2U sala 23, Campus Umuarama, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: de Oliveira-Júnior
authors.affiliation: Laboratório de Nanobiotecnologia, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Rua Acre s/n, Bloco 2E sala 230, Campus Umuarama, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Fabiana de Almeida
authors.initials: Fde A
authors.lastname: Araújo Santos
authors.affiliation: Laboratório de Nanobiotecnologia, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Rua Acre s/n, Bloco 2E sala 230, Campus Umuarama, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Luiz Ricardo
authors.initials: LR
authors.lastname: Goulart
authors.affiliation: Laboratório de Genética, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Rua Acre s/n, Bloco 2E sala 230, Campus Umuarama, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Ueira-Vieira
mesh_terms: D000544:Alzheimer Disease / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood / Q000737:chemistry* / Q000276:immunology*; D018604:Epitope Mapping / Q000379:methods*; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000737:chemistry / Q000276:immunology; D019151:Peptide Library
chemical_list: D001323:Autoantibodies; D000939:Epitopes; D007074:Immunoglobulin G; D019151:Peptide Library