title: The Amoebicidal Effect of Ergosterol Peroxide Isolated from Pleurotus ostreatus.
abstract: Dysentery is an inflammation of the intestine caused by the protozoan parasite Entamoeba histolytica and is a recurrent health problem affecting millions of people worldwide. Because of the magnitude of this disease, finding novel strategies for treatment that does not affect human cells is necessary. Ergosterol peroxide is a sterol particularly known as a major cytotoxic agent with a wide spectrum of biological activities produced by edible and medicinal mushrooms. The aim of this report is to evaluate the amoebicidal activity of ergosterol peroxide (5α, 8α-epidioxy-22E-ergosta-6,22-dien-3β-ol isolated from 5α, 8α-epidioxy-22E-ergosta-6,22-dien-3β-ol) (Jacq.) P. Kumm. f. sp. Florida. Our results show that ergosterol peroxide produced a strong cytotoxic effect against amoebic growth. The inhibitory concentration IC50 of ergosterol peroxide was evaluated. The interaction between E. histolytica and ergosterol peroxide in vitro resulted in strong amoebicidal activity (IC50  = 4.23 nM) that may be due to the oxidatory effect on the parasitic membrane. We also tested selective toxicity of ergosterol peroxide using a cell line CCL-241, a human epithelial cell line isolated from normal human fetal intestinal tissue. To the best of our knowledge, this is the first report on the cytotoxicity of ergosterol peroxide against E. histolytica, which uncovers a new biological property of the lipidic compound isolated from Pleurotus ostreatus (Jacq.) P. Kumm. f. sp. Florida.
authors.affiliation: Laboratorio de Genómica Humana, Facultad de Medicina, Universidad Veracruzana, Calle Médicos y Odontólogos S/N., Col. Unidad del Bosque, Código Postal 91010, Xalapa, Veracruz, Mexico.|Laboratorio de Alta Tecnología de Xalapa, Universidad Veracruzana, Calle Médicos 5, Col. Unidad del Bosque, 91010, Xalapa, Veracruz, Mexico.
authors.forename: Thuluz
authors.initials: T
authors.lastname: Meza-Menchaca
authors.affiliation: Laboratorio de Alta Tecnología de Xalapa, Universidad Veracruzana, Calle Médicos 5, Col. Unidad del Bosque, 91010, Xalapa, Veracruz, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Suárez-Medellín
authors.affiliation: Laboratorio de Alta Tecnología de Xalapa, Universidad Veracruzana, Calle Médicos 5, Col. Unidad del Bosque, 91010, Xalapa, Veracruz, Mexico.
authors.forename: Christian
authors.initials: C
authors.lastname: Del Ángel-Piña
authors.affiliation: Laboratorio de Alta Tecnología de Xalapa, Universidad Veracruzana, Calle Médicos 5, Col. Unidad del Bosque, 91010, Xalapa, Veracruz, Mexico.|Instituto de Ciencias Básicas, Universidad Veracruzana, Luis Castelazo Ayala s/n, Col. Industrial Ánimas, 91190, Xalapa, Veracruz, Mexico.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Trigos
mesh_terms: D000363:Agaricales / Q000737:chemistry; D000563:Amebicides / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D004748:Entamoeba histolytica / Q000187:drug effects*; D004847:Epithelial Cells / Q000187:drug effects; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D020076:Pleurotus / Q000737:chemistry*
chemical_list: D000563:Amebicides; C036071:ergosterol-5,8-peroxide; D004875:Ergosterol
keywords: Pleurotus ostreatus; amoebicidal activity; ergosterol peroxide