title: The influence of lignin on steam pretreatment and mechanical pulping of poplar to achieve high sugar recovery and ease of enzymatic hydrolysis.
abstract: With the goal of enhancing overall carbohydrate recovery and reducing enzyme loading refiner mechanical pulping and steam pretreatment (210°C, 5 min) were used to pretreat poplar wood chips. Neutral sulphonation post-treatment indicated that, although the lignin present in the steam pretreated substrate was less reactive, the cellulose-rich, water insoluble component was more accessible to cellulases and Simons stain. This was likely due to lignin relocation as the relative surface lignin measured by X-ray photoelectron spectroscopy increased from 0.4 to 0.8. The integration of sulphite directly into steam pretreatment resulted in the solubilisation of 60% of the lignin while more than 80% of the carbohydrate present in the original substrate was recovered in the water insoluble fraction after Na2CO3 addition. More than 80% of the sugars present in the original cellulose and xylan could be recovered after 48 h using an enzyme loading of 20 mg protein/g cellulose at a 10% substrate concentration.
authors.affiliation: Forest Products Biotechnology/Bioenergy Group, University of British Columbia, Faculty of Forestry, British Columbia, Canada.
authors.forename: Richard P
authors.initials: RP
authors.lastname: Chandra
authors.affiliation: Forest Products Biotechnology/Bioenergy Group, University of British Columbia, Faculty of Forestry, British Columbia, Canada.
authors.forename: QiuLu
authors.initials: Q
authors.lastname: Chu
authors.affiliation: Forest Products Biotechnology/Bioenergy Group, University of British Columbia, Faculty of Forestry, British Columbia, Canada.
authors.forename: Jinguang
authors.initials: J
authors.lastname: Hu
authors.affiliation: Forest Products Biotechnology/Bioenergy Group, University of British Columbia, Faculty of Forestry, British Columbia, Canada.
authors.forename: Na
authors.initials: N
authors.lastname: Zhong
authors.affiliation: Forest Products Biotechnology/Bioenergy Group, University of British Columbia, Faculty of Forestry, British Columbia, Canada.
authors.forename: Mandy
authors.initials: M
authors.lastname: Lin
authors.affiliation: Clean Fuel Department, Korea Institute of Energy Research, Jeongeup, Jeonbuk 580-185, South Korea.
authors.forename: Jin-Suk
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Forest Products Biotechnology/Bioenergy Group, University of British Columbia, Faculty of Forestry, British Columbia, Canada.
authors.forename: Jack
authors.initials: J
authors.lastname: Saddler
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D002480:Cellulase / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D010450:Endopeptidases; D004950:Esterases / Q000032:analysis; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D032107:Populus / Q000737:chemistry*; D013227:Steam; D013447:Sulfites / Q000737:chemistry; D014867:Water / Q000032:analysis; D014934:Wood / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D013227:Steam; D013447:Sulfites; D014867:Water; D002482:Cellulose; D008031:Lignin; D004950:Esterases; D002480:Cellulase; D010450:Endopeptidases
keywords: Lignin; Mechanical pulping; Simons stain; Steam pretreatment; Sulphonation