title: [Pre-analytical stability before centrifugation of 7 biochemical analytes in whole blood].
abstract: The pre-analytical stability of 7 biochemical parameters (parathyroid hormone -PTH-, vitamins A, C E and D, 1,25-dihydroxyvitamin D and insulin) at +4 °C, was studied on whole blood samples before centrifugation. The impact of freezing at -20°C was also analyzed/performed for PTH and vitamin D. The differences in the results of assays for whole blood samples, being kept for different times between sampling time and analysis, from 9 healthy adults, were compaired by using a Student t test. The 7 analytes investigated remained stable up to 4 hours at +4°C in whole blood. This study showed that it is possible to accept uncentrifuged whole blood specimens kept at +4°C before analysis. PTH is affected by freezing whereas vitamin D is not.
authors.affiliation: Département de biochimie et pharmaco-toxicologie, Hôpital de la Cavale-Blanche, CHRU, Brest, France.
authors.forename: Andreas
authors.initials: A
authors.lastname: Perrier-Cornet
authors.affiliation: Département de biochimie et pharmaco-toxicologie, Hôpital de la Cavale-Blanche, CHRU, Brest, France.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Moineau
authors.affiliation: Pôle de biologie, Hôpital de la Cavale-Blanche, CHRU, Brest, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Narbonne
authors.affiliation: Département de biochimie et pharmaco-toxicologie, Hôpital de la Cavale-Blanche, CHRU, Brest, France, Laboratoire de neurosciences de Brest (LNB), Faculté de médecine, Brest, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Plee-Gautier
authors.affiliation: Département de biochimie et pharmaco-toxicologie, Hôpital de la Cavale-Blanche, CHRU, Brest, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Le Saos
authors.affiliation: Département de biochimie et pharmaco-toxicologie, Hôpital de la Cavale-Blanche, CHRU, Brest, France, Laboratoire de neurosciences de Brest (LNB), Faculté de médecine, Brest, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Carre
mesh_terms: D000328:Adult; D001774:Blood Chemical Analysis; D001800:Blood Specimen Collection; D002498:Centrifugation; D006403:Hematologic Tests; D006801:Humans; D013997:Time Factors
keywords: PTH; centrifugation; freezing; preservation; vitamin D