title: Abnormal increased re-released Hb from RBCs of an intrahepatic bile duct carcinoma patient was detected by electrophoresis release test.
abstract: In this paper, the hemoglobin (Hb) re-released from red blood cells (RBCs) and whole blood of 7 carcinoma patients were studied by using electrophoresis release test (ERT), which was established by our lab. Among the 7 carcinoma patients, the re-released Hb was distinctively increased from an intrahepatic bile duct carcinoma patient during one-dimension isotonic ERT. Different from the others, the result of double-dimension Hb re-release of this intrahepatic bile duct carcinoma patient showed that not only HbA but also HbA2 could be re-released from both RBCs and whole blood. The result of isotonic & hypotonic ERT which was performed at room temperature showed that more Hb could be re-released from both RBCs and whole blood of the intrahepatic bile duct carcinoma patient than that of the normal control. After keeping the samples at 37°C for 1 hour, the re-released Hb from RBCs could still be found more than that of the normal control, but was disappeared completely from the whole blood sample. To our surprise, when the isotonic & hypotonic ERT was repeated 2 days later at 37°C, the re-released Hb from RBCs of the intrahepatic bile duct carcinoma patient was increased only in tube 4-6, and disappeared in the other tube. Further mechanism research work cannot be continued because of the patient's leave, but ERT is speculated to be a useful and effective technology to observe the physiological or pathological change of RBCs, blood or body in the future.
authors.affiliation: Laboratory of Hemoglobin, Baotou Medical College, Baotou, 014060, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.affiliation: Laboratory of Hemoglobin, Baotou Medical College, Baotou, 014060, China.
authors.forename: Lisha
authors.initials: L
authors.lastname: Han
authors.affiliation: Laboratory of Hemoglobin, Baotou Medical College, Baotou, 014060, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Invasive Technology, the Seventh Hospital of Baotou, Baotou, 014010, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
authors.affiliation: Laboratory of Hemoglobin, Baotou Medical College, Baotou, 014060, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Sun
authors.affiliation: Laboratory of Hemoglobin, Baotou Medical College, Baotou, 014060, China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Li
authors.affiliation: Laboratory of Hemoglobin, Baotou Medical College, Baotou, 014060, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Qin
mesh_terms: D000328:Adult; D001650:Bile Duct Neoplasms / Q000097:blood* / Q000473:pathology*; D001653:Bile Ducts, Intrahepatic / Q000473:pathology*; D004586:Electrophoresis; D004912:Erythrocytes / Q000473:pathology*; D006441:Hemoglobin A / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans
chemical_list: D006454:Hemoglobins; D006441:Hemoglobin A
keywords: Hemoglobin; electrophoresis release test; intrahepatic bile duct carcinoma; red blood cell