title: Metabolites of arachidonic acid and linoleic acid in early stages of non-alcoholic fatty liver disease--A pilot study.
abstract: BACKGROUND
  Nonalcoholic fatty liver disease (NAFLD) is a spectrum of liver conditions related to fat infiltration. The role of liver triacylglycerol accumulation in NAFLD is not fully understood.
  
  
  METHODS
  Twenty-four patients, 12 in the first and 12 in the second stage of NAFLD, were prospectively enrolled in this study. Biochemical parameters and eicosanoids (HETE and HODE) were compared between the first and the second stage of hepatic steatosis and the effect of a 6-month dietary intervention on these parameters was evaluated. Eicosanoid profiles were extracted from 0.5 ml of plasma using solid-phase extraction RP-18 SPE columns. The HPLC separations were performed on a 1260 liquid chromatograph.
  
  
  RESULTS
  Patients with stage I NAFLD had a significantly higher level of HDL cholesterol and a lower level of 5-HETE. Patients with grade II steatosis had higher concentrations of 9-HODE. Following the six-month dietary intervention, hepatic steatosis resolved completely in all patients. This resulted in a significant decrease in the concentrations of all eicosanoids (LX4, 16-HETE, 13-HODE, 9-HODE, 15-HETE, 12-HETE, 5-oxoETE, 5-HETE) and key biochemical parameters (BMI, insulin, HOMA-IR, liver enzymes).
  
  
  CONCLUSIONS
  A significant reduction in the analyzed eicosanoids and a parallel reduction in fatty liver confirmed the usefulness of HETE and HODE in the assessment of NAFLD.
authors.affiliation: Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Maciejewska
authors.affiliation: Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Ossowski
authors.affiliation: Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Arleta
authors.initials: A
authors.lastname: Drozd
authors.affiliation: Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Karina
authors.initials: K
authors.lastname: Ryterska
authors.affiliation: Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Jamioł-Milc
authors.affiliation: Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Banaszczak
authors.affiliation: Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kaczorowska
authors.affiliation: Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Sabinicz
authors.affiliation: Department of General, Transplant and Surgery of the Obser, Warszawa, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Raszeja-Wyszomirska
authors.affiliation: Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland. Electronic address: Polandewast@sci.pum.szczecin.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Stachowska
mesh_terms: D000328:Adult; D001095:Arachidonic Acids / Q000097:blood / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D038441:Diet, Mediterranean; D004038:Diet, Reducing; D015536:Down-Regulation; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D008041:Linoleic Acids / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000000981:diagnostic imaging / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease / Q000000981:diagnostic imaging / Q000178:diet therapy / Q000378:metabolism* / Q000503:physiopathology; D050177:Overweight / Q000150:complications / Q000178:diet therapy; D018384:Oxidative Stress; D010865:Pilot Projects; D011446:Prospective Studies; D012074:Remission Induction; D012720:Severity of Illness Index; D014463:Ultrasonography; D015854:Up-Regulation
chemical_list: D001095:Arachidonic Acids; D015415:Biomarkers; D008041:Linoleic Acids
keywords: Biochemical parameters; Eicosanoids; Inflammation; Inflammatory mediators; NAFLD