title: Cloning and expression pattern analysis of BkGRAS2 from Betula kirghisorum.
abstract: GRAS proteins are plant-specific transcription factors that are involved in the regulation of root and shoot growth. Here, we cloned BkGRAS2 from Betula kirghisorum (abbreviated to Bk) and analyzed the physicochemical properties and expression pattern of the encoded protein. BkGRAS2 had an open reading frame of 1614 bp encoding 537 amino acid residues. The deduced BkGRAS2 protein was hydrophilic, and it contained highly conserved VHIID and SAW motifs. BkGRAS1 and BkGRAS2 showed considerable sequence similarities. An expression analysis indicated that BkGRAS2 was expressed in root, stem, and leaf, with the highest level in the leaf. Expression of BkGRAS2 was increased following stress treatment with 0.6% NaHCO3. Transient expression analysis of GFP-BkGRAS2 in onion epidermal cells revealed that the BkGRAS2 protein was localized in the cytoplasm, but could also be detected in the nucleus. Our study provides the basis for future research on the role of the GRAS gene family in B. kirghisorum.
authors.affiliation: College of Forestry, Northeast Forestry University, Harbin, China.
authors.forename: G Y
authors.initials: GY
authors.lastname: Li
authors.affiliation: College of Forestry, Northeast Forestry University, Harbin, China nxyycj@sina.cn.
authors.forename: C J
authors.initials: CJ
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
authors.forename: G J
authors.initials: GJ
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D029662:Betula / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003681:Dehydration; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots; D021381:Protein Transport; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant; D014157:Transcription Factors