title: Robust and convenient analysis of protein thermal and chemical stability.
abstract: We present the software CDpal that is used to analyze thermal and chemical denaturation data to obtain information on protein stability. The software uses standard assumptions and equations applied to two-state and various types of three-state denaturation models in order to determine thermodynamic parameters. It can analyze denaturation monitored by both circular dichroism and fluorescence spectroscopy and is extremely flexible in terms of input format. Furthermore, it is intuitive and easy to use because of the graphical user interface and extensive documentation. As illustrated by the examples herein, CDpal should be a valuable tool for analysis of protein stability.
authors.affiliation: Division of Chemistry, Department of Physics Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden.
authors.forename: Markus
authors.initials: M
authors.lastname: Niklasson
authors.affiliation: Division of Chemistry, Department of Physics Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Andresen
authors.affiliation: Division of Drug Research, Department of Medical and Health Sciences, Linköping University, 581 85 Linköping, Sweden.
authors.forename: Sara
authors.initials: S
authors.lastname: Helander
authors.affiliation: Division of Chemistry, Department of Physics Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden.
authors.forename: Marie G L
authors.initials: MG
authors.lastname: Roth
authors.affiliation: Division of Drug Research, Department of Medical and Health Sciences, Linköping University, 581 85 Linköping, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Zimdahl Kahlin
authors.affiliation: Division of Drug Research, Department of Medical and Health Sciences, Linköping University, 581 85 Linköping, Sweden.
authors.forename: Malin
authors.initials: M
authors.lastname: Lindqvist Appell
authors.affiliation: Division of Chemistry, Department of Physics Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden.
authors.forename: Lars-Göran
authors.initials: LG
authors.lastname: Mårtensson
authors.affiliation: Division of Chemistry, Department of Physics Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden.
authors.forename: Patrik
authors.initials: P
authors.lastname: Lundström
mesh_terms: D002942:Circular Dichroism; D019295:Computational Biology / Q000379:methods*; D008958:Models, Molecular; D011489:Protein Denaturation; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry*; D012984:Software; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D011506:Proteins
keywords: chemical denaturation; circular dichroism; curve fitting; fluorescence; protein denaturation software; protein stability; protein stability software; thermal denaturation