title: Deglycosylation Step to Improve the Identification of Egg Proteins in Art Samples.
abstract: A deglycosylation step using Peptide-N-Glycosidase F (PNGaseF) has been introduced in a standard proteomic protocol to more confidently identify egg based binders. The ingenuity of introducing a PNGaseF digestion was aimed at removing the molecular hindrance, made up by the heavily glycosylated egg proteins, before the protease(s) hydrolysis. This novelty in the protocol resulted in obtaining a significant increase of proteolytic egg peptides thus improving the quality and reliability of egg identification in artwork samples. The protocol has been set up on paint replicas and successfully tested on two historical samples of different origin.
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II , 80126 Napoli, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Vinciguerra
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II , 80126 Napoli, Italy.
authors.forename: Eugenio
authors.initials: E
authors.lastname: Galano
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II , 80126 Napoli, Italy.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Vallone
authors.affiliation: Dipartimento di Studi Umanistici, Università degli Studi di Napoli Federico II , 80133 Napoli, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Greco
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II , 80126 Napoli, Italy.|Distretto ad Alta Tecnologia dei Beni Culturali (DATABENC) Scarl , Napoli, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Vergara
authors.affiliation: Dipartimento di Chimica e Chimica Industriale, Università di Pisa , 56124 Pisa, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Bonaduce
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II , 80126 Napoli, Italy.
authors.forename: Gennaro
authors.initials: G
authors.lastname: Marino
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II , 80126 Napoli, Italy.|Distretto ad Alta Tecnologia dei Beni Culturali (DATABENC) Scarl , Napoli, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Pucci
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II , 80126 Napoli, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Amoresano
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II , 80126 Napoli, Italy.|Distretto ad Alta Tecnologia dei Beni Culturali (DATABENC) Scarl , Napoli, Italy.
authors.forename: Leila
authors.initials: L
authors.lastname: Birolo
mesh_terms: D002853:Chromatography, Liquid; D004527:Egg Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D010151:Paintings; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D004527:Egg Proteins; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase