title: STAT3 contributes to NK cell recognition by modulating expression of NKG2D ligands in adriamycin-resistant K562/AO2 cells.
abstract: Leukemic cells can survive after chemotherapy by acquisition of multidrug resistance genes, but other phenotypes related to escape from immune recognition remain elusive. Adriamycin-resistant K562/AO2 cells are less susceptible to elimination by NK cells compared with wild type K562 cells due to lower expression of NKG2D ligands. Treatment of K562/AO2 cells with STAT3 inhibitor VII resulted in reduced expression of multidrug resistance gene P-glycoprotein, and up-regulation of NKG2D ligands on K562/AO2 cells. Meanwhile, K562/AO2 cells treated with STAT3 inhibitor proliferated less and were more susceptible to killing by NK cells than untreated K562/AO2 cells. The enhanced cytotoxicity of NK cells against K562/AO2 cells was partly blocked by treatment of NK cells with anti-NKG2D antibodies. These data suggest that STAT3 contributes to NK cell recognition by modulating NKG2D ligands in K562/AO2 cells, which may a mechanism by which cells survive and cause relapse of leukemia.
authors.affiliation: Department of Hematology, The Key Medical Subject of Jiangsu, The Affiliated Hospital of Southeast University, Zhongda Hospital, Hunan Road Dingjia Qiao 87, Nanjing, 210009, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Cai
authors.affiliation: Department of Hematology, The Key Medical Subject of Jiangsu, The Affiliated Hospital of Southeast University, Zhongda Hospital, Hunan Road Dingjia Qiao 87, Nanjing, 210009, China. luxuzhang2008@163.com.|Department of Hematology, The Affiliated Hospital of Nanjing Medical University, Changzhou No.2 People's Hospital, Changzhou, China. luxuzhang2008@163.com.
authors.forename: Xuzhang
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Hematology, The Affiliated Hospital of Nanjing Medical University, Changzhou No.2 People's Hospital, Changzhou, China.
authors.forename: Zhuxia
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Department of Hematology, The Affiliated Hospital of Nanjing Medical University, Changzhou No.2 People's Hospital, Changzhou, China.
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Hematology, The Affiliated Hospital of Nanjing Medical University, Changzhou No.2 People's Hospital, Changzhou, China.
authors.forename: Wenmin
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Hematology, The Affiliated Hospital of Nanjing Medical University, Changzhou No.2 People's Hospital, Changzhou, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Rong
authors.affiliation: Department of Hematology, The Affiliated Hospital of Nanjing Medical University, Changzhou No.2 People's Hospital, Changzhou, China.
authors.forename: Lingdi
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Hematology, The Affiliated Hospital of Nanjing Medical University, Changzhou No.2 People's Hospital, Changzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Hematology, The Key Medical Subject of Jiangsu, The Affiliated Hospital of Southeast University, Zhongda Hospital, Hunan Road Dingjia Qiao 87, Nanjing, 210009, China.
authors.forename: Baoan
authors.initials: B
authors.lastname: Chen
mesh_terms: D004317:Doxorubicin; D019008:Drug Resistance, Neoplasm / Q000276:immunology*; D005260:Female; D015973:Gene Expression Regulation, Leukemic / Q000276:immunology*; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000276:immunology* / Q000473:pathology; D007938:Leukemia / Q000276:immunology* / Q000473:pathology; D008297:Male; D055655:NK Cell Lectin-Like Receptor Subfamily K / Q000276:immunology*; D009363:Neoplasm Proteins / Q000276:immunology*; D050796:STAT3 Transcription Factor / Q000276:immunology*
chemical_list: C527085:KLRK1 protein, human; D055655:NK Cell Lectin-Like Receptor Subfamily K; D009363:Neoplasm Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D004317:Doxorubicin
keywords: K562/AO2; NK cells; NKG2D ligands; STAT3