title: Cd34 and Mast Cell Analysis in Normal Oral Mucosa and Different Grades of Oral Squamous Cell Carcinoma: A Comparative Study.
abstract: BACKGROUND
  Oral Squamous Cell Carcinoma (OSCC) remains a serious health problem worldwide. Prognosis of OSCC is poor and long term survival rate still remains below 50%. Angiogenesis or neovascularisation plays an important role in tumour progression and metastasis. Mast cells have been implicated in promoting tumour angiogenesis, especially of digestive tract, little is known in OSCC.
  
  
  OBJECTIVE
  To study the correlation between blood vessel density (BVD) and mast cell density (MCD) in different grades of OSCC.
  
  
  METHODS
  
  
  
  METHODS
  Thirty eight paraffin blocks of different grades of OSCC were retrieved from the department and sections were stained with CD34 followed by counterstaining with toluidine blue. The slides were then analysed using Leica Software (Version 4.5).
  
  
  RESULTS
  Mean BVD and MCD were found to be increased in OSCC as compared to normal mucosa. Increase in BVD with co-current increase in MCD was also observed in different grades of OSCC.
  
  
  CONCLUSIONS
  From our study, it was concluded that, mast cells play a major role in promoting tumour angiogenesis. But, as the grade of the tumour increases, other angiogenic factors may play a more significant role than mast cells in tumour progression.
authors.affiliation: Post Graduate Student, Department of Oral & Maxillofacial Pathology, Sinhgad Dental College & Hospital , Pune, India .
authors.forename: Pargatsingh T
authors.initials: PT
authors.lastname: Kathuriya
authors.affiliation: Assistant Professor, Department of Oral & Maxillofacial Pathology, Sinhgad Dental College & Hospital , Pune, India .
authors.forename: Anirudha R
authors.initials: AR
authors.lastname: Bartake
authors.affiliation: Professor and Head, Department of Oral & Maxillofacial Pathology, Sinhgad Dental College & Hospital , Pune, India .
authors.forename: Sangeeta J
authors.initials: SJ
authors.lastname: Palaskar
authors.affiliation: Assistant Professor, Department of Oral & Maxillofacial Pathology, Sinhgad Dental College & Hospital , Pune, India .
authors.forename: Bindiya R
authors.initials: BR
authors.lastname: Narang
authors.affiliation: Post Graduate Student, Department of Oral & Maxillofacial Pathology, Sinhgad Dental College & Hospital , Pune, India .
authors.forename: Swati S
authors.initials: SS
authors.lastname: Patil
authors.affiliation: Post Graduate Student, Department of Oral & Maxillofacial Pathology, Sinhgad Dental College & Hospital , Pune, India .
authors.forename: Rasika B
authors.initials: RB
authors.lastname: Pawar
keywords: Angiogenesis; Malignant neoplasm Progression