title: Development of recombinant antibody-based enzyme-linked immunosorbent assay (ELISA) for the detection of skatole.
abstract: The occurrence of boar taint and the European Commission recommendation to discontinue the surgical castration of pigs by the year 2018 creates an urgent need for new analytical methods that are simple, affordable, and suitable for field testing. We describe the generation and engineering of a skatole-specific antibody derived from a synthetic antibody library and the development of ELISA for its detection. The immunoassay is capable of detecting skatole with IC50 of 222 μg L(-1), which is within the analytical threshold level suggested for skatole, and with low cross-reactivity interference from other indolic compounds.
authors.affiliation: University of Turku, Department of Biochemistry, Tykistökatu 6 A, FIN-20520, Turku, Finland. Electronic address: jpleiv@utu.fi.
authors.forename: Janne
authors.initials: J
authors.lastname: Leivo
authors.affiliation: University of Turku, Department of Biochemistry, Tykistökatu 6 A, FIN-20520, Turku, Finland.
authors.forename: Joonas
authors.initials: J
authors.lastname: Mäkelä
authors.affiliation: University of Turku, Department of Biochemistry, Tykistökatu 6 A, FIN-20520, Turku, Finland.
authors.forename: Jaana
authors.initials: J
authors.lastname: Rosenberg
authors.affiliation: University of Turku, Department of Biochemistry, Tykistökatu 6 A, FIN-20520, Turku, Finland.
authors.forename: Urpo
authors.initials: U
authors.lastname: Lamminmäki
mesh_terms: D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D007211:Indoles / Q000737:chemistry / Q000276:immunology; D008297:Male; D009812:Odorants / Q000032:analysis; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D012862:Skatole / Q000032:analysis* / Q000276:immunology; D013552:Swine
chemical_list: D007211:Indoles; D057127:Single-Chain Antibodies; D012862:Skatole
keywords: Antibody engineering; Boar taint; ELISA; Hapten; Immunoassay; Phage display