title: Three New Cytotoxic ent-Kaurane Diterpenes from Isodon excisoides.
abstract: Three types of ent-kaurane diterpenoids were isolated from the aerial parts of Isodon excisoides, including three new diterpenoids, 1α,7α,14β-trihydroxy-20-acetoxy-ent-kaur-15-one (1); 1α,7α,14β,18-tetrahydroxy-20-acetoxy-ent-kaur-15-one (2); and 1α-acetoxy-14β-hydroxy-7α,20-epoxy-ent-kaur-16-en-15-one (3); together with six known diterpenes henryin (4); kamebanin (5); reniformin C (6); kamebacetal A (7); kamebacetal B (8); and oridonin (9). The structures of the isolated compounds were elucidated by means of nuclear magnetic resonance spectroscopy and high-resolution mass spectrometry in conjunction with published data for their analogs, as well as their fragmentation patterns. Compounds 5 and 9 were isolated from Isodon excisoides for the first time. To explore the structure-activity relationships of the isolated compounds, they were tested for their cytotoxic effects against five human cancer cell lines: HCT-116, HepG2, A2780, NCI-H1650, and BGC-823. Most of the isolated compounds showed certain cytotoxic activity against the five cancer cell lines with IC50 values ranging from 1.09-8.53 µM. Among the tested compounds, compound 4 exhibited the strongest cytotoxic activity in the tested cell lines, with IC50 values ranging from 1.31-2.07 µM. Compounds 1, 6, and 7 exhibited selective cytotoxic activity.
authors.affiliation: School of pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China. zzdai@163.com.|Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. zzdai@163.com.|Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Zhengzhou 450046, China. zzdai@163.com.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Dai
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. yujiaolong1977@aliyun.com.|National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine, Beijing 100700, China. yujiaolong1977@aliyun.com.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100500, China. yanghanze@imm.ac.cn.
authors.forename: Han-Ze
authors.initials: HZ
authors.lastname: Yang
authors.affiliation: School of pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China. yqlu123@163.com.|Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. yqlu123@163.com.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Lu
authors.affiliation: Henan Province Hospital of TCM, Zhengzhou 450002, China. yuhongyan009@126.com.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Yu
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. huimin_gao@126.com.|Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100500, China. huimin_gao@126.com.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Gao
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. zhmw123@163.com.|Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100500, China. zhmw123@163.com.
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Wang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D045325:HCT116 Cells; D056945:Hep G2 Cells; D006801:Humans; D037602:Isodon / Q000737:chemistry*; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes
keywords: Isodon excisoides; cytotoxic activity; ent-kaurane diterpene; structure-activity relationship