title: Sensitive and rapid detection of pathogenic bacteria in small volumes using impedance spectroscopy technique.
abstract: We illustrate a novel impedance immunosensor which rapidly and sensitively detects typhoid-causing infectious bacteria Salmonella enterica serovar (Salmonella typhi) in 10 μL of sample volume. The bacteria are tagged with gold nanoparticles (Au NPs) via high-affinity antigen-antibody interactions for enhanced signal amplification and selectivity. The cell-particle bioconjugates are then subjected to alternating current (AC) electric fields applied through interdigitated microelectrodes. The immunosensor performance is optimized with respect to electric field frequency, cell concentration, incubation times and the type of blocking agent to achieve a low limit of detection (LOD) of 100 CFU/mL. The approach is extendable to a wide spectrum of clinical diseases and offers an efficient and cost-effective solution for point-of-care diagnosis.
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
authors.forename: Namrata
authors.initials: N
authors.lastname: Pal
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
authors.forename: Shashank
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India. Electronic address: shalinig@chemical.iitd.ac.in.
authors.forename: Shalini
authors.initials: S
authors.lastname: Gupta
mesh_terms: D058491:Bacterial Load / Q000295:instrumentation*; D003199:Computer Systems; D003217:Conductometry / Q000295:instrumentation*; D058266:Dielectric Spectroscopy / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D015203:Reproducibility of Results; D012485:Salmonella typhi / Q000302:isolation & purification*; D018401:Sample Size; D012680:Sensitivity and Specificity
keywords: Biosensor; Detection; Gold nanoparticle; Impedance; Salmonella; Typhoid