title: Detection of Trypanosoma vivax using PCR and LAMP during aparasitemic periods.
abstract: Trypanosoma vivax affects cattle herds in Africa and Americas and has been spreading rapidly in Brazil, through introduction of animals with subclinical infections and without apparent parasitemia, which makes its diagnosis challenging. PCR and LAMP are effective in detecting the presence of T. vivax DNA in situations of low parasitemia. LAMP is simpler and faster technique than PCR, and can be performed in the field, with limited resources. In this study, the capacities of conventional PCR and LAMP for detecting T. vivax in bovine blood samples classified as aparasitemic were evaluated. The capacity of conventional PCR (56.25%) for detecting positive samples was lower than that of LAMP (93.73%). This may influence the choice of screening tests for cattle herds infected with T. vivax.
authors.affiliation: FMVA, UNESP/Araçatuba, Rua Clóvis Pestana 793, 16050-470 Araçatuba, SP, Brazil. Electronic address: fabianocadioli@fmva.unesp.br.
authors.forename: Fabiano Antonio
authors.initials: FA
authors.lastname: Cadioli
authors.affiliation: FCAV, UNESP/Jaboticabal, Via de Acesso Paulo Donato Castellane s/n, 14884-900 Jaboticabal, SP, Brazil.
authors.forename: Otavio Luiz
authors.initials: OL
authors.lastname: Fidelis Junior
authors.affiliation: FCAV, UNESP/Jaboticabal, Via de Acesso Paulo Donato Castellane s/n, 14884-900 Jaboticabal, SP, Brazil.
authors.forename: Paulo Henrique
authors.initials: PH
authors.lastname: Sampaio
authors.affiliation: FMVA, UNESP/Araçatuba, Rua Clóvis Pestana 793, 16050-470 Araçatuba, SP, Brazil.
authors.forename: Giuliana Nascimento
authors.initials: GN
authors.lastname: dos Santos
authors.affiliation: FCAV, UNESP/Jaboticabal, Via de Acesso Paulo Donato Castellane s/n, 14884-900 Jaboticabal, SP, Brazil.
authors.forename: Marcos Rogério
authors.initials: MR
authors.lastname: André
authors.affiliation: FMVA, UNESP/Araçatuba, Rua Clóvis Pestana 793, 16050-470 Araçatuba, SP, Brazil.
authors.forename: Kayo José Garcia de Almeida
authors.initials: KJ
authors.lastname: Castilho
authors.affiliation: FCAV, UNESP/Jaboticabal, Via de Acesso Paulo Donato Castellane s/n, 14884-900 Jaboticabal, SP, Brazil.
authors.forename: Rosangela Zacarias
authors.initials: RZ
authors.lastname: Machado
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis / Q000469:parasitology*; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods; D018512:Parasitemia / Q000662:veterinary*; D016689:Trypanosoma vivax / Q000302:isolation & purification*; D014353:Trypanosomiasis, African / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*
keywords: ELISA; Loop-mediated isothermal amplification; Polymerase chain reaction; Trypanosomiasis