title: Cellular senescence-like features of lung fibroblasts derived from idiopathic pulmonary fibrosis patients.
abstract: Idiopathic pulmonary fibrosis (IPF) is an age-related fatal disease with unknown etiology and no effective treatment. In this study, we show that primary cultures of fibroblasts derived from lung biopsies of IPF patients exhibited (i) accelerated replicative cellular senescence (CS); (ii) high resistance to oxidative-stress-induced cytotoxicity or CS; (iii) a CS-like morphology (even at the proliferative phase); and (iv) rapid accumulation of senescent cells expressing the myofibroblast marker α-SMA. Our findings suggest that CS could serve as a bridge connecting lung aging and its quite frequent outcome -- pulmonary fibrosis, and be an important player in the disease progression. Consequently, targeting senescent cells offers the potential of being a promising therapeutic approach.
authors.affiliation: The Shraga Segal Department of Microbiology, Immunology and Genetics, Center for Multidisciplinary Research on Aging, Ben-Gurion University of the Negev, POB 653, Beer Sheva 84105, Israel.
authors.forename: Hagai
authors.initials: H
authors.lastname: Yanai
authors.affiliation: The Shraga Segal Department of Microbiology, Immunology and Genetics, Center for Multidisciplinary Research on Aging, Ben-Gurion University of the Negev, POB 653, Beer Sheva 84105, Israel.
authors.forename: Albert
authors.initials: A
authors.lastname: Shteinberg
authors.affiliation: Flow Cytometry Unit, Department of Biological services, Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Ziv
authors.initials: Z
authors.lastname: Porat
authors.affiliation: The Shraga Segal Department of Microbiology, Immunology and Genetics, Center for Multidisciplinary Research on Aging, Ben-Gurion University of the Negev, POB 653, Beer Sheva 84105, Israel.|Judea Regional Research and Development Center, Carmel 90404, Israel.
authors.forename: Arie
authors.initials: A
authors.lastname: Budovsky
authors.affiliation: The Shraga Segal Department of Microbiology, Immunology and Genetics, Center for Multidisciplinary Research on Aging, Ben-Gurion University of the Negev, POB 653, Beer Sheva 84105, Israel.
authors.forename: Alex
authors.initials: A
authors.lastname: Braiman
authors.affiliation: Division of Pulmonary Medicine, Department of Internal Medicine II, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Rolf
authors.initials: R
authors.lastname: Zeische
authors.affiliation: The Shraga Segal Department of Microbiology, Immunology and Genetics, Center for Multidisciplinary Research on Aging, Ben-Gurion University of the Negev, POB 653, Beer Sheva 84105, Israel.
authors.forename: Vadim E
authors.initials: VE
authors.lastname: Fraifeld
mesh_terms: D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000368:Aged; D002455:Cell Division; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D016922:Cellular Senescence; D018450:Disease Progression; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans; D008168:Lung / Q000473:pathology; D008875:Middle Aged; D018384:Oxidative Stress; D011658:Pulmonary Fibrosis / Q000473:pathology*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D001616:beta-Galactosidase
keywords: aging; cellular senescence; fibroblasts; idiopathic pulmonary fibrosis; myofibroblasts