title: Characterization of Tn3000, a Transposon Responsible for blaNDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India.
abstract: In Enterobacteriaceae, the blaNDM genes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. In August 2013, we identified NDM-1-producing Escherichia coli and Enterobacter hormaechei strains from a single rectal swab sample from a patient hospitalized in Rio de Janeiro, Brazil, who had no history of travel abroad. Complete DNA sequencing using the Illumina platform and annotation of the two plasmids harboring the blaNDM-1 gene, one from each strain, showed that they belonged to incompatibility groups IncFIIK and IncX3 and harbored a novel transposon named Tn3000. Similar genetic structures have been identified among other isolates in Brazil but also on plasmids from other continents. Our findings suggest that the blaNDM-1 gene may be transmitted by Tn3000 in different parts of the world.
authors.affiliation: School of Pharmacy, University of São Paulo, São Paulo, Brazil.
authors.forename: Juliana Coutinho
authors.initials: JC
authors.lastname: Campos
authors.affiliation: Fleury Group, Microbiology Section, Rio de Janeiro, Brazil.
authors.forename: Maria José Félix
authors.initials: MJ
authors.lastname: da Silva
authors.affiliation: Children's Hospital, Rio de Janeiro, Brazil.
authors.forename: Paulo Roberto Nascimento
authors.initials: PR
authors.lastname: dos Santos
authors.affiliation: School of Pharmacy, University of São Paulo, São Paulo, Brazil.
authors.forename: Elaine Menezes
authors.initials: EM
authors.lastname: Barros
authors.affiliation: School of Pharmacy, University of São Paulo, São Paulo, Brazil.
authors.forename: Mayne de Oliveira
authors.initials: Mde O
authors.lastname: Pereira
authors.affiliation: School of Pharmacy, University of São Paulo, São Paulo, Brazil.
authors.forename: Bruna Mara Silva
authors.initials: BM
authors.lastname: Seco
authors.affiliation: Research Laboratory of Bacterial Resistance, Experimental Research Center, Clinical Hospital of Porto Alegre, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Cibele Massotti
authors.initials: CM
authors.lastname: Magagnin
authors.affiliation: Fleury Group, Microbiology Section, Rio de Janeiro, Brazil.
authors.forename: Leonardo Kalab
authors.initials: LK
authors.lastname: Leiroz
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute, University of São Paulo, São Paulo, Brazil.
authors.forename: Théo Gremen Mimary
authors.initials: TG
authors.lastname: de Oliveira
authors.affiliation: Núcleo de Bacteriologia, GBM/Laboratório Central de Saúde Pública, Lacen-Brasilia, Brasília, Brazil.
authors.forename: Célio
authors.initials: C
authors.lastname: de Faria-Júnior
authors.affiliation: Core Facility for Scientific Research, University of São Paulo, São Paulo, Brazil.
authors.forename: Louise Teixeira
authors.initials: LT
authors.lastname: Cerdeira
authors.affiliation: Research Laboratory of Bacterial Resistance, Experimental Research Center, Clinical Hospital of Porto Alegre, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Afonso Luís
authors.initials: AL
authors.lastname: Barth
authors.affiliation: Microbiology Department, Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Suely Carlos Ferreira
authors.initials: SC
authors.lastname: Sampaio
authors.affiliation: Research Laboratory of Bacterial Resistance, Experimental Research Center, Clinical Hospital of Porto Alegre, Federal University of Rio Grande do Sul, Porto Alegre, Brazil Infectious Diseases Service, Hospital de Clínicas de Porto, Porto Alegre, Brazil.
authors.forename: Alexandre Prehn
authors.initials: AP
authors.lastname: Zavascki
authors.affiliation: Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.affiliation: School of Pharmacy, University of São Paulo, São Paulo, Brazil Fleury Diagnostic Medicine, Microbiology Section, São Paulo, Brazil sampaio@usp.br.
authors.forename: Jorge Luiz Mello
authors.initials: JL
authors.lastname: Sampaio
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001398:Aztreonam / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001938:Brazil; D003227:Conjugation, Genetic; D017124:Conserved Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004754:Enterobacter / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005578:Fosfomycin / Q000494:pharmacology; D006801:Humans; D007194:India; D008826:Microbial Sensitivity Tests; D009018:Morocco; D009390:Nepal; D010957:Plasmids; D012007:Rectum / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D005578:Fosfomycin; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1; C063912:carbapenemase; D001398:Aztreonam