title: miR-584 Suppresses Invasion and Cell Migration of Thyroid Carcinoma by Regulating the Target Oncogene ROCK1.
abstract: BACKGROUND
  Uncovering the target gene of miR-584 to control thyroid carcinoma (TC) invasion and migration is of central importance in the diagnosis, treatment, and prognosis of TC. To validate whether miR-584 has a tumor-suppressive role in thyroid cancer cells by targeting ROCK1, a series of experiments were conducted to figure out the mechanism of action of miR-584.
  
  
  METHODS
  Migration analyses and cell proliferation assays were performed using miR-584-transfected cells. The expression levels of miR-584 in TC were detected by using real-time polymerase chain reaction (PCR). Western blot analyses were conducted to find out the relationship between the tumor suppressor miR-584 and the target oncogene ROCK1 protein expression levels. Wound healing experiments were used to examine the relationships between miR-584 and the migration of thyroid cancer K1 cells and the effects of ROCK1 knockdown on K1 cell motility.
  
  
  RESULTS
  Our results demonstrate that altering the miR-584 levels affects human thyroid cancer cell migration, but has no effect on cell proliferation. The relative ROCK-1 expression levels were 1 and 0.54 in the scrambled-sequence control group and the miR-584 group, respectively. K1 cells transfected with siRNA-ROCK-1 showed weaker cell migration than cells transfected with siRNA-NC (negative control); the cell motility ratios were 18% and 27%, respectively.
  
  
  CONCLUSIONS
  These results indicate that miR-584 could inhibit the expression of ROCK1, and ROCK1 knockdown would further affect the migration ability of K1 cells.
authors.affiliation: Department of Head and Neck Surgery, Fudan University Shanghai Cancer Center, Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.forename: Duan-Shu
authors.initials: DS
authors.lastname: Li
authors.forename: Zhuo-Ying
authors.initials: ZY
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Shen
authors.forename: Tuan-Qi
authors.initials: TQ
authors.lastname: Sun
authors.forename: Qing
authors.initials: Q
authors.lastname: Guan
authors.forename: Yun-Jun
authors.initials: YJ
authors.lastname: Wang
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology*; D054460:rho-Associated Kinases / Q000378:metabolism*
chemical_list: C552781:MIRN584 microRNA, human; D035683:MicroRNAs; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases