title: Effects of CuO nanoparticles on insecticidal activity and phytotoxicity in conventional and transgenic cotton.
abstract: Nanoparticles and transgenic plants are recent scientific developments that require systematic study to understand their potential risks to human health. The effects of CuO nanoparticles (NPs) on Bt-transgenic cotton and conventional cotton are reported here. CuO NPs inhibited the growth, development, nutrient content, and indole-3-acetic acid (IAA) and abscisic acid (ABA) concentrations of transgenic and conventional cotton. Transmission electron microscopy (TEM) images showed CuO NPs aggregated on the epidermis of conventional cotton leaves, whereas it had reached into the cells of transgenic cotton leaves by endocytosis. Most CuO NPs aggregates were found on the root outer epidermis and the rest were located in intercellular spaces of both conventional and Bt-transgenic cottons. CuO NPs enhanced the expression of the exogenous gene encoding of Bt toxin protein in leaves and roots, especially at low CuO NP concentrations, providing an important benefit for Bt cotton insect resistance.
authors.affiliation: College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China; Center for Training, Consultancy and Technology Transfer, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Street, Cau Giay District, Ha Noi City, Viet Nam.
authors.forename: Nhan Le
authors.initials: NL
authors.lastname: Van
authors.affiliation: Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003, USA.
authors.forename: Chuanxin
authors.initials: C
authors.lastname: Ma
authors.affiliation: College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Shang
authors.affiliation: College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China; Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003, USA. Electronic address: ruiyukui@163.com.
authors.forename: Yukui
authors.initials: Y
authors.lastname: Rui
authors.affiliation: College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: Shutong
authors.initials: S
authors.lastname: Liu
authors.affiliation: Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003, USA.
authors.forename: Baoshan
authors.initials: B
authors.lastname: Xing
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D003300:Copper / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity*; D004705:Endocytosis; D003368:Gossypium / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000378:metabolism; D007306:Insecticides / Q000378:metabolism*; D053758:Nanoparticles / Q000633:toxicity*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D030821:Plants, Genetically Modified
chemical_list: D007210:Indoleacetic Acids; D007306:Insecticides; C030737:indoleacetic acid; D000040:Abscisic Acid; D003300:Copper; C030973:cupric oxide
keywords: Bt-transgenic cotton; CuO nanoparticle; Expression of Bt toxin protein; Nutrient element; Phytotoxicity; Plant hormone