title: Romanowsky staining using liquid-based cytology: A pilot study using Cytolyt(®)/HESPANDER(®) processing solution for ThinPrep(®) preparations.
abstract: BACKGROUND
  Liquid-based cytology (LBC) has been used to prepare and examine many types of samples. However, the use of Romanowsky stains for LBC has not yet been evaluated. Herein, we report a technique for the use of the Romanowsky May-Grünwald-Giemsa (MGG) stain using a ThinPrep(®) preparation technique (MGG-LBC).
  
  
  METHODS
  KPL-1 breast cancer cells at a density of 1.25 × 10(5)/20 ml were compared in conventional smear and MGG-LBC preparations. Cell size, nucleus/cytoplasm (N/C) ratio, and morphological findings were investigated. Clinical samples including voided urine and pleural effusion were also examined in MGG-LBC preparations.
  
  
  RESULTS
  Cellularity appeared lower with MGG-LBC compared with Papanicolaou-stained smears using the ThinPrep(®) method, though the cell size and N/C ratio showed similar tendencies. Reactive mesothelial cells, normal urothelial cells, urothelial carcinoma cells, crystals, and bacteria were all clear enough for diagnostic purposes after MGG-LBC.
  
  
  CONCLUSIONS
  Romanowsky staining is necessary for the cytodiagnosis of some conditions. MGG-LBC may contribute to the cytodiagnostic results using LBC preparations.
authors.affiliation: Department of Pathology II, Kansai Medical University, Hirakata, Osaka, Japan.|Division of Diagnostic Cytopathology and Histopathology, Kansai Medical University Takii Hospital, Moriguchi, Osaka, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kinoshita
authors.affiliation: Department of Pathology II, Kansai Medical University, Hirakata, Osaka, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yuri
authors.affiliation: Department of Pathology II, Kansai Medical University, Hirakata, Osaka, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Yoshizawa
authors.affiliation: Division of Surgical Pathology, Hyogo Prefectural Amagasaki Hospital, Amagasaki, Hyogo, Japan.
authors.forename: Kosho
authors.initials: K
authors.lastname: Takasu
authors.affiliation: Department of Pathology II, Kansai Medical University, Hirakata, Osaka, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Emoto
authors.affiliation: Department of Pathology II, Kansai Medical University, Hirakata, Osaka, Japan.
authors.forename: Airo
authors.initials: A
authors.lastname: Tsubura
authors.affiliation: Division of Diagnostic Cytopathology and Histopathology, Kansai Medical University Takii Hospital, Moriguchi, Osaka, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Shikata
mesh_terms: D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000473:pathology*; D003581:Cytodiagnosis / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000473:pathology*; D013194:Staining and Labeling / Q000379:methods*
keywords: CytoLyt/HESPANDER solution; Romanowsky stain; liquid-based cytology; pleural effusion; urine