title: Deficient and excess dietary selenium levels affect growth performance, blood cells apoptosis and liver HSP70 expression in juvenile yellow catfish Pelteobagrus fulvidraco.
abstract: We investigated the effects of deficient and excess dietary selenium (Se) on growth, blood cells apoptosis and liver heat shock protein 70 (HSP70) expression in juvenile yellow catfish (Pelteobagrus fulvidraco). After 8 weeks, yellow catfish (initial weight: 2.12 ± 0.01 g) fed isonitrogenous and isolipid diets containing <0.05 (deficient dietary Se) or 6.5 (excess dietary Se) mg Se/kg displayed a significantly lower weight gain ratio (WGR) than those fed a diet containing 0.23 (normal dietary Se) mg Se/kg. As dietary Se levels increased, liver Se concentration, glutathione peroxidase activity and the hepatosomatic index increased significantly. Plasma glucose concentration was highest in the normal treatment compared with the excess dietary Se treatment. Both deficient and excess dietary Se lead to increased reactive oxygen species (ROS) production and apoptosis ratio in blood cells, whereas only excess dietary Se increased their cytoplasmic free-Ca(2+) (CF-Ca(2+)) concentration. Excess dietary Se also resulted in the highest level of HSP70 expression, thereby possibly providing a protective mechanism against oxidative stress. These results indicate that both deficient and excess dietary Se restrained the growth of juvenile yellow catfish and caused oxidative stress. The overproduction of ROS may act as a signal molecule mediate apoptosis when dietary Se deficiency. Both ROS and CF-Ca(2+) were recorded when dietary Se excess, suggesting that Ca(2+) may be activated by Se and play a major role during Se-induced oxidative stress and cell apoptosis.
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou, 510631, China.|Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors.forename: Jun-Ru
authors.identifier: http://orcid.org/0000-0002-4335-1746
authors.initials: JR
authors.lastname: Hu
authors.affiliation: Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Huang
authors.affiliation: Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors.forename: Guo-Xia
authors.initials: GX
authors.lastname: Wang
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou, 510631, China.
authors.forename: Ying-Xia
authors.initials: YX
authors.lastname: Wu
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou, 510631, China.
authors.forename: Jian-An
authors.initials: JA
authors.lastname: Xian
authors.affiliation: Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou, 510631, China. wanganl@scnu.edu.cn.
authors.forename: An-Li
authors.initials: AL
authors.lastname: Wang
authors.affiliation: Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China. junmcao@163.com.
authors.forename: Jun-Ming
authors.initials: JM
authors.lastname: Cao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001773:Blood Cells / Q000187:drug effects; D001786:Blood Glucose / Q000032:analysis; D002397:Catfishes / Q000254:growth & development / Q000378:metabolism; D004032:Diet; D029941:Fish Proteins / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012643:Selenium / Q000172:deficiency* / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; D029941:Fish Proteins; D018840:HSP70 Heat-Shock Proteins; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D005979:Glutathione Peroxidase; D012643:Selenium
keywords: Apoptosis; Growth; Heat shock protein 70; Selenium (Se); Yellow catfish