title: Targeted PRINT Hydrogels: The Role of Nanoparticle Size and Ligand Density on Cell Association, Biodistribution, and Tumor Accumulation.
abstract: In this Letter, we varied targeting ligand density of an EGFR binding affibody on the surface of two different hydrogel PRINT nanoparticles (80 nm × 320 and 55 nm × 60 nm) and monitored effects on target-cell association, off-target phagocytic uptake, biodistribution, and tumor accumulation. Interestingly, variations in ligand density only significantly altered in vitro internalization rates for the 80 nm × 320 nm particle. However, in vivo, both particle sizes experienced significant changes in biodistribution and pharmacokinetics as a function of ligand density. Overall, nanoparticle size and passive accumulation were the dominant factors eliciting tumor sequestration.
authors.forename: Kevin G
authors.initials: KG
authors.lastname: Reuter
authors.forename: Jillian L
authors.initials: JL
authors.lastname: Perry
authors.forename: Dongwook
authors.initials: D
authors.lastname: Kim
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Luft
authors.forename: Rihe
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Chemical and Biomolecular Engineering, North Carolina State University , Raleigh, North Carolina 27695, United States.|Sloan-Kettering Institute for Cancer Research, Memorial Sloan-Kettering Cancer Center , New York, New York 10021, United States.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: DeSimone
mesh_terms: D004705:Endocytosis; D020100:Hydrogels; D008024:Ligands; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D014018:Tissue Distribution
chemical_list: D020100:Hydrogels; D008024:Ligands
keywords: PRINT; active targeting; ligand density; nanoparticle; pharmacokinetics; tumor accumulation