title: Structure of lpg0406, a carboxymuconolactone decarboxylase family protein possibly involved in antioxidative response from Legionella pneumophila.
abstract: Lpg0406, a hypothetical protein from Legionella pneumophila, belongs to carboxymuconolactone decarboxylase (CMD) family. We determined the crystal structure of lpg0406 both in its apo and reduced form. The structures reveal that lpg0406 forms a hexamer and have disulfide exchange properties. The protein has an all-helical fold with a conserved thioredoxin-like active site CXXC motif and a proton relay system similar to that of alkylhydroperoxidase from Mycobacterium tuberculosis (MtAhpD), suggesting that lpg0406 might function as an enzyme with peroxidase activity and involved in antioxidant defense. A comparison of the size and the surface topology of the putative substrate-binding region between lpg0406 and MtAhpD indicates that the two enzymes accommodate the different substrate preferences. The structural findings will enhance understanding of the CMD family protein structure and its various functions.
authors.affiliation: Institute of Health Sciences and School of Life Sciences, Anhui University, Hefei, Anhui, 230601, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu, Anhui, 241000, China.|School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230026, China.
authors.forename: Yanjin
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Institute of Health Sciences and School of Life Sciences, Anhui University, Hefei, Anhui, 230601, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Institute of Health Sciences and School of Life Sciences, Anhui University, Hefei, Anhui, 230601, China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Gong
authors.affiliation: Institute of Health Sciences and School of Life Sciences, Anhui University, Hefei, Anhui, 230601, China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230026, China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Niu
authors.affiliation: Modern Experiment Technology Center, Anhui University, Hefei, Anhui, 230601, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Health Sciences and School of Life Sciences, Anhui University, Hefei, Anhui, 230601, China.|Modern Experiment Technology Center, Anhui University, Hefei, Anhui, 230601, China.
authors.forename: Honghua
authors.initials: H
authors.lastname: Ge
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002262:Carboxy-Lyases / Q000737:chemistry* / Q000378:metabolism*; D020134:Catalytic Domain; D017124:Conserved Sequence; D004220:Disulfides / Q000378:metabolism*; D016952:Legionella pneumophila / Q000737:chemistry / Q000201:enzymology*; D008958:Models, Molecular; D018384:Oxidative Stress; D011485:Protein Binding; D055503:Protein Multimerization; D017433:Protein Structure, Secondary
chemical_list: D001426:Bacterial Proteins; D004220:Disulfides; D002262:Carboxy-Lyases
keywords: Legionella pneumophila; alkylhydroperoxidase; carboxymuconolactone decarboxylase family; hexameric ring structure; lpg0406