title: Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich.
abstract: Vigna vexillata (L.) A. Rich. (tuber cowpea) is an underutilized crop for consuming its tuber and mature seeds. Wild form of V. vexillata is a pan-tropical perennial herbaceous plant which has been used by local people as a food. Wild V. vexillata has also been considered as useful gene(s) source for V. unguiculata (cowpea), since it was reported to have various resistance gene(s) for insects and diseases of cowpea. To exploit the potential of V. vexillata, an SSR-based linkage map of V. vexillata was developed. A total of 874 SSR markers successfully amplified single DNA fragment in V. vexillata among 1,336 SSR markers developed from Vigna angularis (azuki bean), V. unguiculata and Phaseolus vulgaris (common bean). An F2 population of 300 plants derived from a cross between salt resistant (V1) and susceptible (V5) accessions was used for mapping. A genetic linkage map was constructed using 82 polymorphic SSR markers loci, which could be assigned to 11 linkage groups spanning 511.5 cM in length with a mean distance of 7.2 cM between adjacent markers. To develop higher density molecular linkage map and to confirm SSR markers position in a linkage map, RAD markers were developed and a combined SSR and RAD markers linkage map of V. vexillata was constructed. A total of 559 (84 SSR and 475 RAD) markers loci could be assigned to 11 linkage groups spanning 973.9 cM in length with a mean distance of 1.8 cM between adjacent markers. Linkage and genetic position of all SSR markers in an SSR linkage map were confirmed. When an SSR genetic linkage map of V. vexillata was compared with those of V. radiata and V. unguiculata, it was suggested that the structure of V. vexillata chromosome was considerably differentiated. This map is the first SSR and RAD marker-based V. vexillata linkage map which can be used for the mapping of useful traits.
authors.affiliation: Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie, 514-0102, Japan.
authors.forename: Rusama
authors.initials: R
authors.lastname: Marubodee
authors.affiliation: Genetic Resources Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Ogiso-Tanaka
authors.affiliation: Genetic Resources Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Takehisa
authors.initials: T
authors.lastname: Isemura
authors.affiliation: Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand.
authors.forename: Sompong
authors.initials: S
authors.lastname: Chankaew
authors.affiliation: Genetic Resources Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Akito
authors.initials: A
authors.lastname: Kaga
authors.affiliation: Genetic Resources Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Naito
authors.affiliation: Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie, 514-0102, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ehara
authors.affiliation: Genetic Resources Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Tomooka
mesh_terms: D007887:Fabaceae / Q000235:genetics*; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D014178:Translocation, Genetic
chemical_list: D005819:Genetic Markers