title: Optimization of renal transfection using a renal suction-mediated transfection method in mice.
abstract: BACKGROUND
  We previously developed a suction-mediated transfection method in mice.
  
  
  OBJECTIVE
  The purpose of this study was to optimize the suction-mediated transfection conditions using a pressure-controlled computer system for efficient and safe kidney-targeted gene delivery in mice.
  
  
  METHODS
  Naked pCMV-Luc was injected into the tail vein in mice, and then the right kidney was suctioned by a device of the suction pressure-controlled system. The effects of renal transfection conditions, such as the suction pressure degree, suction pressure waveform and device area were evaluated by measuring luciferase expression. In addition, renal injury was examined.
  
  
  RESULTS
  The renal suction-mediated transfection method at -30 kPa showed high transgene expression. The renal suction waveform did not affect the transfection activity. Under the optimized conditions, the high transgene expression was mostly observed at the renal suctioned site. The transfection conditions used did not induce histological defects or increases in two renal injury biomarkers (Kidney injury molecule-1 mRNA and Clusterin mRNA).
  
  
  CONCLUSIONS
  We have clarified the transfection conditions for efficient and safe transfection in the kidney using the suction-mediated transfection method in mice.
authors.affiliation: a Department of Drug Delivery Research , Graduate School of Pharmaceutical Sciences, Kyoto University , Kyoto , Japan .
authors.forename: Yota
authors.initials: Y
authors.lastname: Taniguchi
authors.affiliation: b Department of Pharmaceutical Informatics , Graduate School of Biomedical Sciences, Nagasaki University , Nagasaki , Japan .
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kawakami
authors.affiliation: b Department of Pharmaceutical Informatics , Graduate School of Biomedical Sciences, Nagasaki University , Nagasaki , Japan .
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fuchigami
authors.affiliation: b Department of Pharmaceutical Informatics , Graduate School of Biomedical Sciences, Nagasaki University , Nagasaki , Japan .
authors.forename: Natsuko
authors.initials: N
authors.lastname: Oyama
authors.affiliation: a Department of Drug Delivery Research , Graduate School of Pharmaceutical Sciences, Kyoto University , Kyoto , Japan .
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Yamashita
authors.affiliation: c Department of Mechanical Engineering , Ritsumeikan University , Shiga , Japan .
authors.forename: Satoshi
authors.initials: S
authors.lastname: Konishi
authors.affiliation: d Department of Biotechnology , Graduate School of Engineering, Nagoya University , Aichi , Japan , and.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Shimizu
authors.affiliation: a Department of Drug Delivery Research , Graduate School of Pharmaceutical Sciences, Kyoto University , Kyoto , Japan .|e Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University , Kyoto , Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Hashida
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D007668:Kidney / Q000378:metabolism*; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010957:Plasmids / Q000378:metabolism; D011312:Pressure; D012333:RNA, Messenger / Q000378:metabolism; D013396:Suction / Q000379:methods*; D014162:Transfection / Q000379:methods*
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D008156:Luciferases
keywords: Gene therapy; kidney; naked pDNA; tissue suction; transfection