title: Bacterial nucleators: actin' on actin.
abstract: The actin cytoskeleton is a key target of numerous microbial pathogens, including protozoa, fungi, bacteria and viruses. In particular, bacterial pathogens produce and deliver virulence effector proteins that hijack actin dynamics to enable bacterial invasion of host cells, allow movement within the host cytosol, facilitate intercellular spread or block phagocytosis. Many of these effector proteins directly or indirectly target the major eukaryotic actin nucleator, the Arp2/3 complex, by either mimicking nucleation promoting factors or activating upstream small GTPases. In contrast, this review is focused on a recently identified class of effector proteins from Gram-negative bacteria that function as direct actin nucleators. These effector proteins mimic functional activities of formins, WH2-nucleators and Ena/VASP assembly promoting factors demonstrating that bacteria have coopted the complete set of eukaryotic actin assembly pathways. Structural and functional analyses of these nucleators have revealed several motifs and/or mechanistic activities that are shared with eukaryotic actin nucleators. However, functional effects of these proteins during infection extend beyond plain actin polymerization leading to interference with other host cell functions such as vesicle trafficking, cell cycle progression and cell death. Therefore, their use as model systems could not only help in the understanding of the mechanistic details of actin polymerization but also provide novel insights into the connection between actin dynamics and other cellular pathways.
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Joana N
authors.initials: JN
authors.lastname: Bugalhão
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Luís Jaime
authors.initials: LJ
authors.lastname: Mota
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal irinafranco@fct.unl.pt.
authors.forename: Irina S
authors.initials: IS
authors.lastname: Franco
mesh_terms: D000199:Actins / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000378:metabolism* / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D055503:Protein Multimerization; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D000199:Actins; D037521:Virulence Factors
keywords: actin; bacterial virulence; effector protein; nucleation; secretion system