title: Allelic diversity of KIR3DL1/3DS1 in a southern Chinese population.
abstract: The inhibitory KIR3DL1 and the activating KIR3DS1 segregate as alleles of the same locus. KIR3DL1 is highly diversified at the allele level and KIR3DL1 alleles exhibit varied levels of expression and ligand binding affinity resulting in varied degrees of NK cell inhibition. Previous studies have shown that the KIR3DL1/3DS1 polymorphism associated with viral infection, cancer and transplantation. However, little is known about the population distribution of KIR3DL1/3DS1 alleles in Chinese. The present study examined allelic diversity of KIR3DL1/3DS1 in a southern Chinese population (N=306) using PCR-SSP and sequencing based typing. The presence of KIR3DL1 and KIR3DS1 were detected in 97.1% and 34.0% of the tested individuals respectively. A total of 10 KIR3DL1 alleles (including 2 novel ones) and 6 KIR3DS1 alleles (including 5 novel ones) were identified. Common KIR3DL1 alleles (>10%) were KIR3DL1*01502 (74.8%), KIR3DL1*00501 (23.9%) and KIR3DL1*00701 (15.7%). KIR3DS1*01301 was the predominant KIR3DS1 allele with other KIR3DS1 alleles only sporadically observed. The knowledge of the allelic polymorphism of KIR3DL1/3DS1 may help to better understand the role played by KIR3DL1/3DS1 in associated diseases and clinical transplantation in southern Chinese.
authors.affiliation: Immunogenetics Laboratory, Shenzhen Blood Center, Shenzhen, Guangdong 518035, China. Electronic address: zhihui_deng@aliyun.com.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Immunogenetics Laboratory, Shenzhen Blood Center, Shenzhen, Guangdong 518035, China. Electronic address: jianxinzhen1988@qq.com.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhen
authors.affiliation: College of Medicine, Xi'An Jiaotong University, Xi'an, Shaanxi 710049, China. Electronic address: zhubofeng7372@126.com.
authors.forename: Bofeng
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Immunogenetics Laboratory, Shenzhen Blood Center, Shenzhen, Guangdong 518035, China. Electronic address: hypuris@163.com.
authors.forename: Guobing
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Immunogenetics Laboratory, Shenzhen Blood Center, Shenzhen, Guangdong 518035, China. Electronic address: Yuqiong7867@aliyun.com.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Immunogenetics Laboratory, Shenzhen Blood Center, Shenzhen, Guangdong 518035, China. Electronic address: riverch888@aliyun.com.
authors.forename: Daming
authors.initials: D
authors.lastname: Wang
authors.affiliation: Immunogenetics Laboratory, Shenzhen Blood Center, Shenzhen, Guangdong 518035, China. Electronic address: yunpingxu1982@aliyun.com.
authors.forename: Yunping
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Immunogenetics Laboratory, Shenzhen Blood Center, Shenzhen, Guangdong 518035, China. Electronic address: littleduckmei@163.com.
authors.forename: Liumei
authors.initials: L
authors.lastname: He
authors.affiliation: Immunogenetics Laboratory, Shenzhen Blood Center, Shenzhen, Guangdong 518035, China. Electronic address: luliang@126.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Lu
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002681:China; D005260:Female; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D008297:Male; D054341:Receptors, KIR3DL1 / Q000235:genetics*; D054349:Receptors, KIR3DS1 / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D054341:Receptors, KIR3DL1; D054349:Receptors, KIR3DS1
keywords: Diversity; KIR3DL1/3DS1 locus; Killer cell immunoglobulin-like receptor (KIR); Sequencing-based typing