title: Identification of novel proteins differentially expressed in pluripotent embryonic stem cells and differentiated cells.
abstract: Mammalian pluripotent stem cells possess properties of self-renewal and pluripotency. These abilities are maintained by the strict regulation of pluripotent stem cell-specific transcription factor network and unique properties of chromatin in the stem cells. Although these major signaling pathways robustly control the characteristics of stem cells, other regulatory factors, such as metabolic pathways, are also known to modulate stem cell proliferation and differentiation. In this study, we fractionated protein samples from mouse embryonic stem (ES) cells cultured with or without the leukemia inhibitory factor (LIF). Protein expression was quantified by 2-dimensional differential gel electrophoresis (2D-DIGE). In total, 44 proteins were identified as being differentially expressed in the pluripotent stem cells and the differentiated cells. Surprisingly, half of the identified proteins were the proteins localized in mitochondria, which supply cellular energy and regulate cell cycle, development, and cell death. Some of these identified proteins are involved in the metabolic function and the regulation of pluripotency. Further analysis of the identified proteins could provide new information for the manipulation of pluripotency in ES cells.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo.
authors.forename: Kei
authors.initials: K
authors.lastname: Enomoto
authors.forename: Kanako
authors.initials: K
authors.lastname: Watanabe-Susaki
authors.forename: Megumi
authors.initials: M
authors.lastname: Kowno
authors.forename: Hitomi
authors.initials: H
authors.lastname: Takada
authors.forename: Atsushi
authors.initials: A
authors.lastname: Intoh
authors.forename: Yuko
authors.initials: Y
authors.lastname: Yamanaka
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hirano
authors.forename: Hiromu
authors.initials: H
authors.lastname: Sugino
authors.forename: Makoto
authors.initials: M
authors.lastname: Asashima
authors.forename: Akira
authors.initials: A
authors.lastname: Kurisaki
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D053595:Embryonic Stem Cells / Q000737:chemistry*; D053605:Leukemia Inhibitory Factor / Q000032:analysis; D051379:Mice; D039904:Pluripotent Stem Cells / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*
chemical_list: D053605:Leukemia Inhibitory Factor