title: Aortic wall proteomic analysis in spontaneously hypertensive rats with a blood pressure decrease induced by 6-week load-free swimming.
abstract: Decreased arterial compliance is one of the earliest detectable manifestations of adverse structural and functional changes within the vessel wall in hypertension. The proteomic approach is a powerful technique to analyze a complex mixture of proteins in various settings. Physical activity level was negatively associated with blood pressure. Sixteen 4-week-old male spontaneously hypertensive rats (SHR) and 16 Wistar-Kyoto (WKY) rats were randomly divided into four groups: i) SHR exercise group, ii) SHR rest group, iii) WKY exercise group and iv) WKY rest group. In the SHR and WKY exercise groups, rats were treated with a 6-week load-free swimming protocol (1 h/day, 5 days/week). The blood pressure of the rats was tested by the CODATM2single non-invasive blood pressure measurement appliance. After the 6-week swimming protocol, the total aorta excluding abdominal aorta was extracted. The proteins were separated by two-dimensional gel electrophoresis and identified via LC-mass spectrometry (MS)/MS. After 6-week load-free swimming, blood pressure decreased in the SHRs. Compared with sedentary SHRs, 11 spots on the 2D-gel showed a significant difference in exercised SHRs. Nine of these were chosen for further identification. There were 5 upregulated proteins (long-chain specific acyl-CoA dehydrogenase, heat shock protein β-1, isocitrate dehydrogenase subunit α, actin, α cardiac muscle 1 preprotein and calmodulin isoform 2) and 4 downregulated proteins (adipocyte-type fatty acid-binding protein, tubulin β-2C chain, 78 kDa glucose-regulated protein precursor and mimecan). Proteomics is an effective method to identify the target proteins of exercise intervention for hypertension.
authors.affiliation: Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Tianjin University of Sport, Tianjin 300381, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Feng
authors.affiliation: Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Health and Exercise Science, Tianjin University of Sport, Tianjin 300381, P.R. China.
authors.forename: Derong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Tianjin University of Sport, Tianjin 300381, P.R. China.
authors.forename: Yungang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Tianjin University of Sport, Tianjin 300381, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Family Planning, The Second Affiliated Hospital of Tianjin Medical University, Tianjin 300211, P.R. China.
authors.forename: Y U
authors.initials: YU
authors.lastname: Zhang
authors.affiliation: School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P.R. China.
authors.forename: Junzhen
authors.initials: J
authors.lastname: Tan
authors.affiliation: Department of Graduate, Tianjin University of Sport, Tianjin 300381, P.R. China.
authors.forename: Wen
authors.initials: W
authors.lastname: Fang
authors.affiliation: Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Tianjin University of Sport, Tianjin 300381, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
keywords: 6-week load-free swimming; aortic wall; hypertension; proteomic analysis