title: N-palmitoyl serotonin alleviates scopolamine-induced memory impairment via regulation of cholinergic and antioxidant systems, and expression of BDNF and p-CREB in mice.
abstract: N-Palmitoyl-5-hydroxytryptamines (Pal-5HT), a cannabinoid, has recently been reported to express anti-allergic and anti-inflammatory actions in RBL-2H3 cells, and ameliorate glutamate-induced cytotoxicity in HT-22 cells. In this study, we examined the effect of Pal-5HT on deficits of learning and memory induced by scopolamine in mice. Memory performance was evaluated using Morris water maze test and passive avoidance test. Activities of acetylcholinesterase (AChE) and choline acetyltransferase (ChAT), level of oxidative stress markers, and expression of brain-derived neurotrophic factor (BDNF), phosphorylation of cAMP response element-binding protein (p-CREB) were determined. Loss of neuronal cells in hippocampus was evaluated by histological examinations. Pal-5HT significantly improved the amnesia in the behavioral assessment. Pal-5HT regulated cholinergic function by inhibiting scopolamine-induced elevation of AChE activity and decline of ChAT activity. Pal-5HT suppressed oxidative stress by increasing activities of glutathione peroxidase (GPx), glutathione reductase (GR) or NAD(P)H quinine oxidoreductase-1 (NQO-1) and lowering MDA level. Additionally, it prevented against scopolamine-induced expression of iNOS and COX-2. Moreover, Pal-5HT suppressed the death of neuronal cells in CA1 and CA3 regions, while it restored expression of p-CREB and BDNF in hippocampus. Taken together, Pal-5HT is suggested to ameliorate deficits of memory and learning through regulation of cholinergic function, activation of antioxidant systems as well as restoration of BDNF and p-CREB expression. From these, Pal-5HT may be a potential candidate to prevent against neurodegeneration related to the memory deficit.
authors.affiliation: Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: A Young
authors.initials: AY
authors.lastname: Min
authors.affiliation: Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Choon Nan
authors.initials: CN
authors.lastname: Doo
authors.affiliation: Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Son
authors.affiliation: Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Nak Yun
authors.initials: NY
authors.lastname: Sung
authors.affiliation: Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Kun Jong
authors.initials: KJ
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Dai-Eun
authors.initials: DE
authors.lastname: Sok
authors.affiliation: Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, South Korea. Electronic address: mrkim@cnu.ac.kr.
authors.forename: Mee Ree
authors.initials: MR
authors.lastname: Kim
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001362:Avoidance Learning / Q000187:drug effects; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000139:chemically induced / Q000188:drug therapy*; D008813:Mice, Inbred ICR; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D012601:Scopolamine / Q000009:adverse effects*; D012701:Serotonin / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D019208:Brain-Derived Neurotrophic Factor; C492004:Creb1 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; D012701:Serotonin; D012601:Scopolamine; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D016660:NAD(P)H Dehydrogenase (Quinone); C491732:Nqo1 protein, mouse; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase
keywords: Antioxidant system; BDNF; Cholinergic function; Pal-5HT; Scopolamine; p-CREB