title: Clinicopathological significance and prognostic value of Wilms' tumor gene expression in colorectal cancer.
abstract: BACKGROUND
  Colorectal cancer (CRC) is one of the most commonly diagnosed cancers, and novel effective treatments and diagnostic tools are urgently required.
  
  
  OBJECTIVE
  The selection of appropriate targeting tumor-associated antigens (TAAs) is critical for immunotherapy. Therefore, we analyzed TAA expression levels and investigated their relationship with clinical factors in adjacent normal mucosa (ANM) and CRC tissue.
  
  
  METHODS
  We obtained specimens of CRC primary tumors and matched ANM from 137 patients with CRC who underwent surgical resection. The mRNA levels of seven TAAs, Wilms' tumor gene (WT1), kinetochore associated-2 (KNTC2), cell division cycle associated-1 (CDCA1), M phase phosphoprotein-1 (MPHOSPH1), DEP domain-containing 1 (DEPDC1), 34-kDa translocase of the outer mitochondrial membrane (TOMM34) and ring finger protein-43 (RNF43), were analyzed using quantitative real-time reverse transcription-polymerase chain reaction, and their relationships with clinicopathological factors and the cell cycle were analyzed.
  
  
  RESULTS
  The expression levels of all seven TAAs were significantly higher in CRC tissues than in ANM. Expression levels of WT1 in CRC tissues did not correlate with the cell cycle. Furthermore, WT1 expression in CRC tissues was significantly related to tumor progression, lymph node metastasis, distant metastasis and clinical stage.
  
  
  CONCLUSIONS
  WT1 is a potential marker for prognosis and tumor progression in CRC.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Tsurumi University, Yokohama, Japan.|Department of Rheumatology and Clinical Immunology, Clinical Research Center for Rheumatology and Allergy, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Japan.
authors.forename: Yukinaga
authors.initials: Y
authors.lastname: Miyata
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Tsurumi University, Yokohama, Japan.|Department of Rheumatology and Clinical Immunology, Clinical Research Center for Rheumatology and Allergy, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Kumagai
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Tsurumi University, Yokohama, Japan.|Department of Rheumatology and Clinical Immunology, Clinical Research Center for Rheumatology and Allergy, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nagaoka
authors.affiliation: Department of Rheumatology and Clinical Immunology, Clinical Research Center for Rheumatology and Allergy, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Kitaura
authors.affiliation: Department of Surgery Sagamihara National Hospital, National Hospital Organization, Sagamihara, Japan.
authors.forename: Goro
authors.initials: G
authors.lastname: Kaneda
authors.affiliation: Department of Surgery Sagamihara National Hospital, National Hospital Organization, Sagamihara, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Kanazawa
authors.affiliation: Section of Biological Science Research Center for Odontology, Nippon Dental University School of Dentistry, Tokyo, Japan.
authors.forename: Satsuki
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Tsurumi University, Yokohama, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Hamada
authors.affiliation: Department of Rheumatology and Clinical Immunology, Clinical Research Center for Rheumatology and Allergy, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Suzuki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025721:WT1 Proteins / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D012333:RNA, Messenger; D025721:WT1 Proteins; C583751:WT1 protein, human
keywords: Colorectal cancer; Wilms' tumor gene; tumor-associated antigens