title: Identifying Patient-Specific Pathology in Osteoarthritis Development Based on MicroCT Analysis of Subchondral Trabecular Bone.
abstract: The goal of this study was to identify alternative mechanisms of osteoarthritis pathology by analyzing subchondral bone. Femoral condyle samples were collected from post-menopausal female patients with knee osteoarthritis undegoing total knee arthroplasty. In the majority of patients, subchondral trabecular bone volume doubled under a region of the medial femoral condyle with full-thickness cartilage deterioration. However, in a subset of patients the bone volume in this region remained constant. This subset also had larger areas of vascular penetration in the calcified cartilage of the lateral condyle concurrent with increased vascular endothelial growth factor expression. Subtyping by subchondral bone characteristics identified a unique population, which lacked the sclerotic bone characteristic of late-stage osteoarthritis. Identification of subtypes within the osteoarthritis population allows investigation of alternate disease pathologies.
authors.affiliation: School of Biomedical Engineering, Science & Health Systems, Drexel University, Philadelphia, Pennsylvania; Department of Orthopaedic Surgery, Drexel University College of Medicine, Philadelphia, Pennsylvania.
authors.forename: Marla J
authors.initials: MJ
authors.lastname: Steinbeck
authors.affiliation: Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania.
authors.forename: Peter T
authors.initials: PT
authors.lastname: Eisenhauer
authors.affiliation: Rothman Institute at Thomas Jefferson University, Philadelphia, Pennsylvania.
authors.forename: Mitchell G
authors.initials: MG
authors.lastname: Maltenfort
authors.affiliation: Rothman Institute at Thomas Jefferson University, Philadelphia, Pennsylvania.
authors.forename: Javad
authors.initials: J
authors.lastname: Parvizi
authors.affiliation: Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania.
authors.forename: Theresa A
authors.initials: TA
authors.lastname: Freeman
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D019645:Arthroplasty, Replacement, Knee; D002358:Cartilage, Articular / Q000473:pathology; D018450:Disease Progression; D005260:Female; D005269:Femur / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007719:Knee Joint / Q000473:pathology*; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000473:pathology* / Q000601:surgery*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D055114:X-Ray Microtomography
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: VEGF; microCT; osteoarthritis; osteoclasts; subchondral bone