title: A Novel Sucrose-Regulatory MADS-Box Transcription Factor GmNMHC5 Promotes Root Development and Nodulation in Soybean (Glycine max [L.] Merr.).
abstract: The MADS-box protein family includes many transcription factors that have a conserved DNA-binding MADS-box domain. The proteins in this family were originally recognized to play prominent roles in floral development. Recent findings, especially with regard to the regulatory roles of the AGL17 subfamily in root development, have greatly broadened their known functions. In this study, a gene from soybean (Glycine max [L.] Merr.), GmNMHC5, was cloned from the Zigongdongdou cultivar and identified as a member of the AGL17 subfamily. Real-time fluorescence quantitative PCR analysis showed that GmNMHC5 was expressed at much higher levels in roots and nodules than in other organs. The activation of expression was first examined in leaves and roots, followed by shoot apexes. GmNMHC5 expression levels rose sharply when the plants were treated under short-day conditions (SD) and started to pod, whereas low levels were maintained in non-podding plants under long-day conditions (LD). Furthermore, overexpression of GmNMHC5 in transgenic soybean significantly promoted lateral root development and nodule building. Moreover, GmNMHC5 is upregulated by exogenous sucrose. These results indicate that GmNMHC5 can sense the sucrose signal and plays significant roles in lateral root development and nodule building.
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement and MOA Key Lab of Soybean Biology (Beijing), Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Haidian District, Beijing 100081, China. hnaulw@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement and MOA Key Lab of Soybean Biology (Beijing), Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Haidian District, Beijing 100081, China. hxdtjnu@126.com.|School of Life Science, Beijing Institute of Technology, 5 Zhongguancun South Street, Haidian District, Beijing 100081, China. hxdtjnu@126.com.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Han
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement and MOA Key Lab of Soybean Biology (Beijing), Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Haidian District, Beijing 100081, China. zhan_ge_edu@sina.com.|School of Life Science, Beijing Institute of Technology, 5 Zhongguancun South Street, Haidian District, Beijing 100081, China. zhan_ge_edu@sina.com.
authors.forename: Ge
authors.initials: G
authors.lastname: Zhan
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement and MOA Key Lab of Soybean Biology (Beijing), Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Haidian District, Beijing 100081, China. zhaozf2009@126.com.|School of Life Science, Beijing Institute of Technology, 5 Zhongguancun South Street, Haidian District, Beijing 100081, China. zhaozf2009@126.com.
authors.forename: Zhenfang
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: School of Life Science, Beijing Institute of Technology, 5 Zhongguancun South Street, Haidian District, Beijing 100081, China. fengyj@bit.edu.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Feng
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement and MOA Key Lab of Soybean Biology (Beijing), Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Haidian District, Beijing 100081, China. wucunxiang@caas.cn.
authors.forename: Cunxiang
authors.initials: C
authors.lastname: Wu
mesh_terms: D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D053204:Root Nodules, Plant / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D013025:Glycine max / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D026161:MADS Domain Proteins; D010940:Plant Proteins; D014157:Transcription Factors; D013395:Sucrose
keywords: Glycine max; GmNMHC5; MADS-box protein; lateral roots development; nodule building; sucrose