title: Characterization of Achromobacter Species in Cystic Fibrosis Patients: Comparison of bla(OXA-114) PCR Amplification, Multilocus Sequence Typing, and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.
abstract: Molecular methodologies were used to identify 28 Achromobacter spp. from patients with cystic fibrosis (CF). Multilocus sequence typing (MLST) identified 17 Achromobacter xylosoxidans isolates (all bla(OXA-114) positive), nine Achromobacter ruhlandii isolates (all bla(OXA-114) positive), one Achromobacter dolens isolate, and one Achromobacter insuavis isolate. All less common species were misidentified as A. xylosoxidans by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Chronic colonization by clonally related A. ruhlandii isolates was demonstrated.
authors.forename: Elenice R A
authors.initials: ER
authors.lastname: Rodrigues
authors.forename: Alex G
authors.initials: AG
authors.lastname: Ferreira
authors.forename: Robson S
authors.initials: RS
authors.lastname: Leão
authors.forename: Cassiana C F
authors.initials: CC
authors.lastname: Leite
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Carvalho-Assef
authors.forename: Rodolpho M
authors.initials: RM
authors.lastname: Albano
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Marques
mesh_terms: D042402:Achromobacter / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003550:Cystic Fibrosis / Q000150:complications*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D058885:Multilocus Sequence Typing / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001618:beta-Lactamases