title: Oxymatrine inhibited cell proliferation by inducing apoptosis in human lung cancer A549 cells.
abstract: To investigate the inhibition effect of oxymatrine induces human lung cancer A549 cells apoptosis. The A549 cells were cultured for 24 h, than the various concentration of oxymatrine (2 mmol/L, 4 mmol/L, 8 mmol/L, 15 mmol/L) were added into different experimental group cells, and 5-fluorouracil were added into the positive control group cells for 12 h, 24 h, 36 h, 48 h respectively. The A549 cells inhibition rate, apoptosis, and the expression of Bcl-2 and Bax were examined by MTT method, Annexin V/PI double staining method, real-time quantitative PCR and western blot, respectively. At same time, the morphological changes of A549 cells were observed with an inverted microscope. In the range of 2 mmol/L~15 mmol/L, oxymatrine had obvious inhibition effects on the proliferation of A549 cells. Compared with the negative control group, it has significantly different (P<0.01). There was remarkably the time- and dose-dependent correlation. After A549 cells were treated with 8 mmol/L oxymatrine for 24 h, the morphological change of cell apoptosis was observed and the extent of apoptosis was quantified by flow cytometry. Furthermore, the expression of Bcl-2 was reduced and the expression of Bax was increased remarkably (P<0.05). Oxymatrine has significant inhibition effects on the cells proliferation and the effects showed time-dependent and dose-dependent. Oxymatrine can induce apoptosis of the A549 cells by regulating the expression of Bcl-2 and Bax.
authors.affiliation: School of Basic Medical, Henan University of Traditional Chinese Medicine, Zhengzhou, China.
authors.forename: Baiyan
authors.initials: B
authors.lastname: Wang
authors.affiliation: School of Basic Medical, Henan University of Traditional Chinese Medicine, Zhengzhou, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Han
authors.affiliation: School of Basic Medical, Henan University of Traditional Chinese Medicine, Zhengzhou, China.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage*; D000970:Antineoplastic Agents / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D011807:Quinolizines / Q000008:administration & dosage*; D016896:Treatment Outcome; D051028:bcl-2-Associated X Protein / Q000378:metabolism*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D011807:Quinolizines; D051028:bcl-2-Associated X Protein; C494826:BAX protein, human; C037573:oxymatrine; D000093842:Matrines
keywords: A549 cells; Bax; Bcl-2; Oxymatrine; apoptosis; proliferation inhibition