title: In situ carcinoma developed over oral lichen planus: a case report with analysis of BUB3, p16, p53, Ki67 and SOX4 expression.
abstract: Oral lichen planus (OLP) represents a common mucocutaneous disease. Various authors have suggested that OLP has malignant potential; however, the mechanisms involved in malignant transformation have not yet been elucidated. A 79-year-old man presented a white lesion for five months in the buccal mucosa diagnosed as OLP. After two months using 0.05% clobetasol ointment for treatment, the lesion became ulcerated. A new biopsy of the same lesion was performed, and histological analysis showed an in situ oral carcinoma (ISOC). An immunohistochemistry panel was performed, and p16 expression was negative in OLP, however, it showed weak cytoplasmic staining in ISOC. There was strong nuclear BUB3 staining in both OLP and ISOC areas. p53 showed less intense nuclear staining in both regions. Ki67 was negative in OLP area, but showed nuclear staining in the ISOC. SOX4 was negative in both studied areas. BUB3 expression, first reported in this case, and the p16 expression may suggest some influence of these genes on pathogenesis or malignant potential of OLP.
authors.affiliation: Faculty of Medicine, University of Brasília, Brasília, DF, BR.
authors.forename: Eduardo Augusto
authors.initials: EA
authors.lastname: Rosa
authors.affiliation: Department of Dentistry, Faculty of Health Sciences, University of Brasília, Brasília, DF, BR.
authors.forename: Erica Negrini
authors.initials: EN
authors.lastname: Lia
authors.affiliation: Department of Dentistry, Faculty of Health Sciences, University of Brasília, Brasília, DF, BR.
authors.forename: Sergio Bruzadelli
authors.initials: SB
authors.lastname: Macedo
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Brasília, Brasília, DF, BR.
authors.forename: Rivadavio Fernandes Batista de
authors.initials: RF
authors.lastname: Amorim
mesh_terms: D000368:Aged; D002278:Carcinoma in Situ / Q000209:etiology / Q000473:pathology*; D018797:Cell Cycle Proteins / Q000032:analysis; D002471:Cell Transformation, Neoplastic; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis; D019942:Genes, p16; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D017676:Lichen Planus, Oral / Q000150:complications / Q000473:pathology*; D008297:Male; D009062:Mouth Neoplasms / Q000209:etiology / Q000473:pathology*; D000075223:Poly-ADP-Ribose Binding Proteins; D055751:SOXC Transcription Factors / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: C489444:BUB3 protein, human; D018797:Cell Cycle Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D019394:Ki-67 Antigen; D000075223:Poly-ADP-Ribose Binding Proteins; C083431:SOX4 protein, human; D055751:SOXC Transcription Factors; D016159:Tumor Suppressor Protein p53