title: Overexpression and biological function of TMEM48 in non-small cell lung carcinoma.
abstract: Transmembrane protein 48 (TMEM48), localized to nuclear pore complexes (NPCs), has been reported crucial for NPC assembly. Alterations in NPC members have been reported in several malignancies. The present study was aimed to elucidate the expression and biological function of TMEM48 in non-small cell lung carcinoma (NSCLC). Here, TMEM48 expression level was higher in NSCLC tissues than that in the adjacent normal tissues. Moreover, higher TMEM48 expression was correlated with a more advanced tumor stage, lymph node metastasis, bigger tumor size tumor stage, and shorter survival time. Knockdown of TMEM48 in NSCLC cell lines, A549 and H1299, inhibited cell proliferation and significantly increased cells population in G1 phase. Gene set enrichment analysis (GSEA) showed that cell cycle pathway was correlative with the TMEM48 expression. Additionally, real-time PCR and western blot analysis revealed that several cell cycle and DNA replication genes, including Cyclin B1, CDK1, CDC6, PCNA, and RCF4, were reduced after TMEM48 knockdown. Additionally, inhibition of TMEM48 in NSCLC cells significantly stimulated cell apoptosis, while notably repressed cell adhesion, migration, invasion, and tumorigenicity in nude mice. Our data provide insight into the biological relevance of TMEM48 in NSCLC progression and highlight its usefulness as a prognostic factor and potential therapeutic target in NSCLC.
authors.affiliation: Department of Thoracic Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 100 Haining Rd, Shanghai, 200080, China.
authors.forename: Wenliang
authors.initials: W
authors.lastname: Qiao
authors.affiliation: Department of Thoracic Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 100 Haining Rd, Shanghai, 200080, China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Han
authors.affiliation: Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Tian
authors.affiliation: Department of Thoracic Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 100 Haining Rd, Shanghai, 200080, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Cardiothoracic Surgery, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Min
authors.affiliation: Department of Thoracic Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 100 Haining Rd, Shanghai, 200080, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Thoracic Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 100 Haining Rd, Shanghai, 200080, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Thoracic Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 100 Haining Rd, Shanghai, 200080, China.
authors.forename: Wenzhuo
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Pulmonary Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, 241 West Huaihai Rd, Shanghai, 200030, China. xiong_li_Wen@126.com.
authors.forename: Liwen
authors.initials: L
authors.lastname: Xiong
authors.affiliation: Department of Thoracic Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 100 Haining Rd, Shanghai, 200080, China. xklinqiang@hotmail.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lin
mesh_terms: D000072283:A549 Cells; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D002448:Cell Adhesion / Q000235:genetics; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D016193:G1 Phase / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D028861:Nuclear Pore Complex Proteins / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: C510249:NDC1 protein, human; D028861:Nuclear Pore Complex Proteins
keywords: Cell cycle; Metastasis; NSCLC; Proliferation; TMEM48