title: Mutations Found in embCAB, embR, and ubiA Genes of Ethambutol-Sensitive and -Resistant Mycobacterium tuberculosis Clinical Isolates from China.
abstract: To better understand the molecular mechanisms of Ethambutol (EMB) resistance, the mutant hot spot region of five genes (embB, embA, embC, embR, and ubiA) was amplified and sequenced in 109 EMB-resistant and 153 EMB-susceptible clinical isolates from China. Twenty-seven EMB-susceptible isolates were found to have nonsynonym mutations, 23 of which were in embB. The mutations occurred most frequently in embB (85.3%, 93) and were seldom in embC (2.8%, 3), embA (3.7%, 4), embR (3.7%, 4), and ubiA (8.3%, 9) in EMB-resistant isolates. For the embB gene, 63 isolates showed mutations at embB306, 20 at embB406, nine at embB497, and five at embB354 in EMB-resistant isolates. In addition, the particular mutants at embB406 and embB497 indicated both high levels of EMB resistance (MICs > 5 μg/mL) and broad anti-TB drug resistance spectrums. Our data supported the facts that embB306 could be used as a marker for EMB resistance with a sensitivity of 57.8% and a specificity of 78.8%.
authors.affiliation: Molecular Laboratory, Beijing Chest Hospital, Capital Medical University, 97 Machang Road, Tongzhou District, Beijing 101149, China ; Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, 97 Machang Road, Tongzhou District, Beijing 101149, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Molecular Laboratory, Beijing Chest Hospital, Capital Medical University, 97 Machang Road, Tongzhou District, Beijing 101149, China ; Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, 97 Machang Road, Tongzhou District, Beijing 101149, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Jia
authors.affiliation: Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, 97 Machang Road, Tongzhou District, Beijing 101149, China ; National Tuberculosis Clinical Laboratory, Beijing Chest Hospital, Capital Medical University, 97 Machang Road, Tongzhou District, Beijing 101149, China.
authors.forename: Hairong
authors.initials: H
authors.lastname: Huang
authors.affiliation: Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, 97 Machang Road, Tongzhou District, Beijing 101149, China ; National Tuberculosis Clinical Laboratory, Beijing Chest Hospital, Capital Medical University, 97 Machang Road, Tongzhou District, Beijing 101149, China.
authors.forename: Zhaogang
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Molecular Laboratory, Beijing Chest Hospital, Capital Medical University, 97 Machang Road, Tongzhou District, Beijing 101149, China ; Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, 97 Machang Road, Tongzhou District, Beijing 101149, China.
authors.forename: Zongde
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D002681:China; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004977:Ethambutol / Q000494:pharmacology*; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D004977:Ethambutol