title: Host antioxidant enzymes and TLR-2 neutralization modulate intracellular survival of Staphylococcus aureus: Evidence of the effect of redox balance on host pathogen relationship during acute staphylococcal infection.
abstract: Staphylococcus aureus is an important pathogen in bone disease and innate immune recognition receptor, TLR-2 is reported to be crucial for inflammatory bone loss. Role of TLR-2 in bacterial clearance and cytokine response to S. aureus infection in murine bone marrow macrophages has been reported but the role of host derived ROS in host-pathogen relationship still remains an obvious question. In the present study, blocking of SOD and catalase in TLR-2 neutralized fresh bone marrow cells (FBMC) with Diethyldithiocarbamic acid (DDC) and 3-Amino-1,2,4-triazole (ATZ), separately, during acute S. aureus infection, produces moderate level of ROS and limits inflammation as compared with only TLR-2 non-neutralized condition and leads to decreased bacterial count compared with only TLR-2 neutralized condition. In summary, host SOD and catalase modulates ROS generation, cytokine levels and TLR-2 expression in FBMCs during acute S. aureus infection which might be useful in the alleviation of S. aureus infection and bone loss.
authors.affiliation: Department of Physiology, Immunology Laboratory, University of Calcutta, University Colleges of Science and Technology, APC Road, Calcutta 700009, West Bengal, India.
authors.forename: Ajeya
authors.initials: A
authors.lastname: Nandi
authors.affiliation: Department of Physiology, Immunology Laboratory, University of Calcutta, University Colleges of Science and Technology, APC Road, Calcutta 700009, West Bengal, India. Electronic address: biswa_dev2@yahoo.com.
authors.forename: Biswadev
authors.initials: B
authors.lastname: Bishayi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D054884:Host-Pathogen Interactions; D051379:Mice; D050296:Microbial Viability; D010084:Oxidation-Reduction; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology* / Q000502:physiology; D013482:Superoxide Dismutase / Q000378:metabolism*; D051195:Toll-Like Receptor 2 / Q000378:metabolism*
chemical_list: D000975:Antioxidants; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Antioxidant inhibitors; Cytokine; Murine fresh bone marrow cells; S. aureus infection; Toll like receptor-2