title: Glucose Transporter Type 4 Redistribution on the Membrane Induced by Insulin through Akt in Hydrocortisone Treatment in Rat Skeletal Muscles.
abstract: Hydrocortisone is a growth hormone frequently used in the treatment of low back pain. Hydrocortisone treatment has an anti-inflammation effect, which also inactivates glucose transporter type 4 (GLUT4) by p38 mitogen-activated protein kinase (MAPK) inhibition. Translocation of GLUT4 regulates body glucose homeostasis and muscle repair and is induced by insulin. In this study, 56 SD rats were divided into seven groups, and were treated with insulin or hydrocortisone in sedentary or exercise training groups. The muscle proteins and biochemical blood parameters were analyzed after 7 days of treatments. The results showed that the serum glucose increased in hydrocortisone treatment accompanied by GLUT4 inactivation in both the sedentary and exercise training rats. In the exercise training groups, GLUT4 was redistributed on the plasma membrane on co-treatment with insulin and hydrocortisone through Akt phosphorylation. Insulin treatment exerted a compensatory feedback effect on the GLUT4 translocation on hydrocortisone co-treatment, which was the cause of GLUT4 inactivation.
authors.affiliation: Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan, Republic of China.|School of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, Republic of China.|Division of Neurosurgery, Department of Surgery, Changhua Christian Hospital, Changhua 50006, Taiwan, Republic of China.
authors.forename: Chien-Min
authors.initials: CM
authors.lastname: Chen
authors.affiliation: Department of Nursing, College of Medicine and Nursing, Hungkuang University, Taichung 43302, Taiwan, Republic of China.
authors.forename: Lian
authors.initials: L
authors.lastname: Chiu
authors.affiliation: Department of Plastic and Reconstructive Surgery, China Medical University and China Medical University Hospital, Taichung 40447, Taiwan, Republic of China.
authors.forename: Hung-Chi
authors.initials: HC
authors.lastname: Chen
authors.affiliation: Division of Neurosurgery, Department of Surgery, Changhua Christian Hospital, Changhua 50006, Taiwan, Republic of China.
authors.forename: Chun-Yuan
authors.initials: CY
authors.lastname: Cheng
authors.affiliation: Graduate Institute of Immunology, China Medical University, Taichung 40402, Taiwan, Republic of China.
authors.forename: Woei-Cherng
authors.initials: WC
authors.lastname: Shyu
authors.affiliation: Division of Neurosurgery, Tungs' Taichung Metroharbor Hospital, Taichung 43503, Taiwan, Republic of China.
authors.forename: Chii-Wen
authors.initials: CW
authors.lastname: Chou
authors.affiliation: Graduate Institute of Immunology, China Medical University, Taichung 40402, Taiwan, Republic of China.|Center for Neuropsychiatry, China Medical University Hospital, Taichung 40402, Taiwan, Republic of China.
authors.forename: Cheng-You
authors.initials: CY
authors.lastname: Lu
authors.affiliation: Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan, Republic of China.|Institute of Veterinary Clinical Science, School of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan, Republic of China.
authors.forename: Chung-Tien
authors.initials: CT
authors.lastname: Lin
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D004347:Drug Interactions; D051275:Glucose Transporter Type 4 / Q000378:metabolism*; D006854:Hydrocortisone / Q000494:pharmacology* / Q000627:therapeutic use; D007004:Hypoglycemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D007328:Insulin / Q000494:pharmacology* / Q000627:therapeutic use; D018482:Muscle, Skeletal / Q000187:drug effects*; D010805:Physical Conditioning, Animal; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D051275:Glucose Transporter Type 4; D007004:Hypoglycemic Agents; D007328:Insulin; C495694:Slc2a4 protein, rat; D051057:Proto-Oncogene Proteins c-akt; D048051:p38 Mitogen-Activated Protein Kinases; D006854:Hydrocortisone