title: Stability indicating RP-HPLC method for simultaneous determination of piroxicam and ofloxacin in binary combination.
abstract: A simple and precise RP-HPLC method was developed for simultaneous determination of piroxicam and ofloxacin in pharmaceutical formulations and human serum. Optimum separations of piroxicam, ofloxacin and stress-induced degradation products were achieved by use of Hypersil BDS C8 column (250 x 4.6mm, 5 μm). The mobile phase was a mixture of acetonitrile: 0.012M K2HPO4: 0.008M sodium citrate (both buffers mixed and pH adjusted to 2.8) (50:25:25 v/v/v) delivered at flow rate of 1.5 mL min⁻¹ using DAD at 254 nm. Response was linear function of concentration over the ranges of 70-130 mg mL⁻¹ for piroxicam and ofloxacin (r² ≥ 0.999). The method efficiently separated the analytical peaks from degradation products with acceptable tailing and resolution. The developed method was successfully used for concurrent analysis of piroxicam and ofloxacin in pharmaceutical formulations, human serum and in vitro drug interaction studies.
authors.affiliation: Department of Chemistry, Government College University, Lahore, Pakistan.
authors.forename: Peter
authors.initials: P
authors.lastname: John
authors.affiliation: Department of Chemistry, Government College University, Lahore, Pakistan.
authors.forename: Waqar
authors.initials: W
authors.lastname: Azeem
authors.affiliation: Department of Chemistry, H. H. Campus, University of Gujrat, Gujrat, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ashfaq
authors.affiliation: Department of Chemistry, Government College University, Lahore, Pakistan.
authors.forename: Islam Ullah
authors.initials: IU
authors.lastname: Khan
authors.affiliation: Department of Chemistry, Government College University, Lahore, Pakistan.
authors.forename: Syed Naeem
authors.initials: SN
authors.lastname: Razzaq
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004338:Drug Combinations; D004355:Drug Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015242:Ofloxacin / Q000737:chemistry*; D010894:Piroxicam / Q000737:chemistry*
chemical_list: D004338:Drug Combinations; D010894:Piroxicam; D015242:Ofloxacin