title: Rosiglitazone protects rat liver against acute liver injury associated with the NF-κB signaling pathway.
abstract: Rosiglitazone, which is mainly used in the treatment of diabetes mellitus, is also involved in the regulation of inflammation. The peroxisome proliferator-activated receptor (PPAR)-γ receptor subtype appears to play a pivotal role in the regulation of inflammation. However, the exact mechanism for the protective effects of rosiglitazone against inflammation such as liver injury remains unclear. The aim of this study was to investigate the effects of rosiglitazone on inflammation in the liver of rats treated with D-GaIN/LPS. Male Sprague-Dawley rats were injected with D-GaIN/LPS with or without pre-administration of rosiglitazone (3, 10, or 30 mg/kg, intraperitoneal injection). Our data showed that rosiglitazone significantly inhibited D-GaIN/LPS-induced hepatotoxicity in a dose-dependent manner, as indicated by both diagnostic indicators of liver damage (serum aminotransferase activities) and histopathological analysis. Western blot analysis showed that rosiglitazone significantly decreased protein expression levels of COX-2 and production of pro-inflammatory markers, including TNF-α and IL-6, in D-GaIN/LPS-treated rat liver. The results indicated that the inhibition of D-GaIN/LPS-induced inflammation by rosiglitazone can be attributed, at least partially, to its capacity to regulate the the immunoregulatory transcription factor nuclear factor kappa B (NF-κB) signaling pathway.
authors.affiliation: a School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei, Anhui, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: a School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei, Anhui, People's Republic of China.
authors.forename: Yuan-Jie
authors.initials: YJ
authors.lastname: Lin
authors.affiliation: a School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei, Anhui, People's Republic of China.
authors.forename: Xu-Ya
authors.initials: XY
authors.lastname: Zhou
authors.affiliation: b Institute of Clinical Pharmacology,Anhui Medical University, 81 Meishan Road, Hefei, Anhui, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: a School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei, Anhui, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004195:Disease Models, Animal; D018836:Inflammation Mediators / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077154:Rosiglitazone; D015398:Signal Transduction / Q000187:drug effects; D045162:Thiazolidinediones / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D018836:Inflammation Mediators; D015850:Interleukin-6; D016328:NF-kappa B; D020011:Protective Agents; D045162:Thiazolidinediones; D014409:Tumor Necrosis Factor-alpha; D000077154:Rosiglitazone; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: COX-2; D-GaIN/LPS; D-GalN/LPS; IL-6; NF-κB; TNF-α; dommage hépatique; inflammation; liver injury; rosiglitazone