title: Kinetics on Demand Is a Simple Mathematical Solution that Fits Recorded Caffeine-Induced Luminal SR Ca2+ Changes in Smooth Muscle Cells.
abstract: The process of Ca2+ release from sarcoplasmic reticulum (SR) comprises 4 phases in smooth muscle cells. Phase 1 is characterized by a large increase of the intracellular Ca2+ concentration ([Ca2+]i) with a minimal reduction of the free luminal SR [Ca2+] ([Ca2+]FSR). Importantly, active SR Ca2+ ATPases (SERCA pumps) are necessary for phase 1 to occur. This situation cannot be explained by the standard kinetics that involves a fixed amount of luminal Ca2+ binding sites. A new mathematical model was developed that assumes an increasing SR Ca2+ buffering capacity in response to an increase of the luminal SR [Ca2+] that is called Kinetics-on-Demand (KonD) model. This approach can explain both phase 1 and the refractory period associated with a recovered [Ca2+]FSR. Additionally, our data suggest that active SERCA pumps are a requisite for KonD to be functional; otherwise luminal SR Ca2+ binding proteins switch to standard kinetics. The importance of KonD Ca2+ binding properties is twofold: a more efficient Ca2+ release process and that [Ca2+]FSR and Ca2+-bound to SR proteins ([Ca2+]BSR) can be regulated separately allowing for Ca2+ release to occur (provided by Ca2+-bound to luminal Ca2+ binding proteins) without an initial reduction of the [Ca2+]FSR.
authors.affiliation: Unidad Monterrey, Cinvestav, Apodaca, Nuevo Leon, Mexico.
authors.forename: Norma C
authors.initials: NC
authors.lastname: Perez-Rosas
authors.affiliation: Departamento de Farmacobiología, Unidad Sur, Cinvestav, Mexico City, Mexico.
authors.forename: Norma L
authors.initials: NL
authors.lastname: Gomez-Viquez
authors.affiliation: Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima, Colima, Mexico.
authors.forename: Adan
authors.initials: A
authors.lastname: Dagnino-Acosta
authors.affiliation: Unidad Monterrey, Cinvestav, Apodaca, Nuevo Leon, Mexico.
authors.forename: Moises
authors.initials: M
authors.lastname: Santillan
authors.affiliation: Departamento de Bioquímica, Cinvestav, Mexico City, Mexico.
authors.forename: Agustín
authors.initials: A
authors.lastname: Guerrero-Hernandez
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D006168:Guinea Pigs; D007700:Kinetics; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000187:drug effects* / Q000378:metabolism
chemical_list: D002110:Caffeine; D002118:Calcium