title: Methyl groups as NMR probes for biomolecular interactions.
abstract: Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that methodological advances and biological insights go hand in hand. We envision that future methyl TROSY applications will continue to provide unique information regarding intermolecular interactions, even for very large eukaryotic protein complexes that are often highly asymmetric.
authors.affiliation: Research group 'Mechanisms of Ubiquitin-dependent Cell Signaling', Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany.
authors.forename: Silke
authors.initials: S
authors.lastname: Wiesner
authors.affiliation: Research group 'NMR Spectroscopy of Large Complexes', Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany. Electronic address: remco.sprangers@tuebingen.mpg.de.
authors.forename: Remco
authors.initials: R
authors.lastname: Sprangers
mesh_terms: D001665:Binding Sites; D018360:Crystallography, X-Ray; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011506:Proteins