title: Phenotypic and Transcriptomic Analyses of Autotetraploid and Diploid Mulberry (Morus alba L.).
abstract: Autopolyploid plants and their organs are often larger than their diploid counterparts, which makes them attractive to plant breeders. Mulberry (Morus alba L.) is an important commercial woody plant in many tropical and subtropical areas. In this study, we obtained a series of autotetraploid mulberry plants resulting from a colchicine treatment. To evaluate the effects of genome duplications in mulberry, we compared the phenotypes and transcriptomes of autotetraploid and diploid mulberry trees. In the autotetraploids, the height, breast-height diameter, leaf size, and fruit size were larger than those of diploids. Transcriptome data revealed that of 21,229 expressed genes only 609 (2.87%) were differentially expressed between diploids and autotetraploids. Among them, 30 genes were associated with the biosynthesis and signal transduction of plant hormones, including cytokinin, gibberellins, ethylene, and auxin. In addition, 41 differentially expressed genes were involved in photosynthesis. These results enhance our understanding of the variations that occur in mulberry autotetraploids and will benefit future breeding work.
authors.affiliation: Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, 133 Yiheng Road, Dongguan Village, Tianhe District, Guangzhou 510610, Guangdong, China. daifanwei2011@163.com.
authors.forename: Fanwei
authors.initials: F
authors.lastname: Dai
authors.affiliation: Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, 133 Yiheng Road, Dongguan Village, Tianhe District, Guangzhou 510610, Guangdong, China. jiangwzh1982@126.com.
authors.forename: Zhenjiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, 133 Yiheng Road, Dongguan Village, Tianhe District, Guangzhou 510610, Guangdong, China. guoqingluogd@163.com.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Luo
authors.affiliation: Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, 133 Yiheng Road, Dongguan Village, Tianhe District, Guangzhou 510610, Guangdong, China. tangcuiming@126.com.
authors.forename: Cuiming
authors.initials: C
authors.lastname: Tang
mesh_terms: D004171:Diploidy; D018506:Gene Expression Regulation, Plant; D031625:Morus / Q000033:anatomy & histology* / Q000235:genetics* / Q000502:physiology; D010641:Phenotype; D010788:Photosynthesis; D000069600:Plant Breeding; D010937:Plant Growth Regulators / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D057891:Tetraploidy; D059467:Transcriptome
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins
keywords: autotetraploid; mulberry; phenotype; plant hormone; transcriptome