title: Expression of pluripotency markers in the bovine uterus with adenomyosis.
abstract: BACKGROUND
  Adenomyosis is a proliferative uterine dysfunction with unknown aetiology. One possible mechanism of its development involves disturbances in stem cell differentiation in uterine tissue. Previously, we identified pluripotent/multipotent cells in the bovine uterus, therefore our present study focused on determining expression of pluripotency markers, NANOG, OCT4 and SOX2, in bovine adenomyotic tissues and cells.
  
  
  RESULTS
  Immunolocalisation revealed protein expression of NANOG, OCT4 and SOX2 in both normal and adenomyotic uteri. mRNA expression for NANOG and OCT4 was increased in tissues obtained from uteri with adenomyosis compared to controls, but at the protein level there were no significant differences. mRNA expression for all three pluripotency markers was higher in myometrial cells isolated from uteri with adenomyotic lesions than in those isolated from normal uteri. The protein level of NANOG and SOX2 was decreased in stromal cells from adenomyotic tissues, whereas the level of OCT4 and SOX2 was increased in myometrial cells obtained from dysfunctional uteri.
  
  
  CONCLUSIONS
  The results indicate significant changes in expression of pluripotency markers in adenomyotic compared to normal uteri, which suggest the involvement of uterine stem cells in adenomyosis.
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland. m.lupicka@pan.olsztyn.pl.
authors.forename: Martyna
authors.initials: M
authors.lastname: Łupicka
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland. b.socha@pan.olsztyn.pl.
authors.forename: Barbara
authors.initials: B
authors.lastname: Socha
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland. a.szczepanska@pan.olsztyn.pl.
authors.forename: Agata
authors.initials: A
authors.lastname: Szczepańska
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland. a.korzekwa@pan.olsztyn.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Korzekwa
mesh_terms: D062788:Adenomyosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D005260:Female; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis* / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000378:metabolism* / Q000473:pathology; D055748:SOXB1 Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014599:Uterus / Q000378:metabolism* / Q000473:pathology
chemical_list: D015415:Biomarkers; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors