title: Gastric stability and oral bioavailability of colistin sulfate in pigs challenged or not with Escherichia coli O149: F4 (K88).
abstract: The aim of the present study was to investigate the in vitro gastric stability of colistin sulfate (CS) and its antimicrobial activity against Escherichia coli and to study the impact of ETEC O149: F4 (K88) infection in pigs on CS intestinal absorption. The stability profile of CS was evaluated in a simulated gastric fluid (SGF). Antimicrobial activity of CS and its degradation products were examined in a 96-well polystyrene microplate model. The effect of experimental infection with ETEC O149: F4 on CS intestinal absorption was determined by quantification of CS systemic concentration using a validated LC-MS/MS method. A rapid degradation of CS accompanied by an increase in CS antimicrobial activity by comparison with non-degraded CS (P<0.0001) was observed in SGF. Additionally, CS levels were not quantifiable in systemic circulation using a highly sensitive method and concurrent oral challenge did not affect CS absorption in an induction model of subclinical post-weaning diarrhea (PWD).
authors.affiliation: Chaire de recherche en salubrité des viandes (CRSV), Canada; Groupe de recherche et d'enseignement en salubrité alimentaire (GRESA), Canada; Centre de recherche en infectiologie porcine et avicole (CRIPA), Canada.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Rhouma
authors.affiliation: Groupe de recherche en pharmacologie animale du Québec, Canada.
authors.forename: Francis
authors.initials: F
authors.lastname: Beaudry
authors.affiliation: Chaire de recherche en salubrité des viandes (CRSV), Canada; Groupe de recherche et d'enseignement en salubrité alimentaire (GRESA), Canada; Centre de recherche en infectiologie porcine et avicole (CRIPA), Canada.
authors.forename: William
authors.initials: W
authors.lastname: Thériault
authors.affiliation: Chaire de recherche en salubrité des viandes (CRSV), Canada; Groupe de recherche et d'enseignement en salubrité alimentaire (GRESA), Canada; Centre de recherche en infectiologie porcine et avicole (CRIPA), Canada.
authors.forename: Nadia
authors.initials: N
authors.lastname: Bergeron
authors.affiliation: Chaire de recherche en salubrité des viandes (CRSV), Canada; Groupe de recherche et d'enseignement en salubrité alimentaire (GRESA), Canada; Centre de recherche en infectiologie porcine et avicole (CRIPA), Canada.
authors.forename: Sylvette
authors.initials: S
authors.lastname: Laurent-Lewandowski
authors.affiliation: Groupe de recherche et d'enseignement en salubrité alimentaire (GRESA), Canada; OIE Reference Laboratory for Escherichia coli (EcL), Faculté de médecine vétérinaire - Université de Montréal, 3200 rue Sicotte, Saint-Hyacinthe, QC J2S 7C6, Canada.
authors.forename: John Morris
authors.initials: JM
authors.lastname: Fairbrother
authors.affiliation: Chaire de recherche en salubrité des viandes (CRSV), Canada; Groupe de recherche et d'enseignement en salubrité alimentaire (GRESA), Canada; Centre de recherche en infectiologie porcine et avicole (CRIPA), Canada; Groupe de recherche en pharmacologie animale du Québec, Canada. Electronic address: ann.letellier@umontreal.ca.
authors.forename: Ann
authors.initials: A
authors.lastname: Letellier
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics / Q000627:therapeutic use*; D001682:Biological Availability; D003091:Colistin / Q000493:pharmacokinetics / Q000627:therapeutic use*; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D054307:Enterotoxigenic Escherichia coli / Q000187:drug effects*; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy / Q000382:microbiology*; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D003091:Colistin
keywords: Antimicrobial activity; Colistin sulfate; E. coli; Gastric stability; Intestinal absorption; Pigs