title: Engineering Biosynthesis Mechanisms for Diversifying Polyhydroxyalkanoates.
abstract: Polyhydroxyalkanoates (PHA) are a family of diverse biopolyesters synthesized by bacteria. PHA diversity, as reflected by its monomers, homopolymers, random and block copolymers, as well as functional polymers, can now be generated by engineering the three basic synthesis pathways including the acetoacetyl-CoA pathway, in situ fatty acid synthesis, and/or β-oxidation cycles, as well as PHA synthase specificity. It is now possible to tailor the PHA structures via genome editing or process engineering. The increasing PHA diversity and maturing PHA production technology should lead to more focused research into their low-cost and/or high-value applications.
authors.affiliation: Ministry of Education Key Lab of Bioinformatics, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, China; Center for Nano and Micro Mechanics, Tsinghua University, Beijing 100084, China; Beijing Key Laboratory of Protein Therapeutics, Tsinghua University, Beijing 100084, China. Electronic address: chengq@mail.tsinghua.edu.cn.
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Lab of Bioinformatics, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, China.
authors.forename: Ivan
authors.initials: I
authors.lastname: Hajnal
authors.affiliation: Ministry of Education Key Lab of Bioinformatics, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
authors.affiliation: Ministry of Education Key Lab of Bioinformatics, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lv
authors.affiliation: Ministry of Education Key Lab of Bioinformatics, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Ye
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics; D049921:Cupriavidus / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D020632:Halomonas / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D060847:Metabolic Engineering; D010084:Oxidation-Reduction; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000235:genetics; D000091402:Prohibitins; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D043367:Ralstonia / Q000235:genetics / Q000378:metabolism
chemical_list: D000214:Acyl Coenzyme A; D005227:Fatty Acids; C000717328:PHB protein, human; D054813:Polyhydroxyalkanoates; D000091402:Prohibitins; C010667:acetoacetyl CoA; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase
keywords: PHA; PHB; biopolymers; synthetic biology; β-oxidation