title: Effect of Radix Trichosanthis and Trichosanthin on Hepatitis B Virus in HepG2.2.15 Cells.
abstract: Radix Trichosanthis is a Chinese herbal medicine that has great medical value and pharmacological actions. There is already a long history of using the plant Radix Trichosanthis as treatment for hepatitis B virus in China. This research mainly focused on investigating the therapeutic effect of different extracts from Radix Trichosanthis on hepatitis B virus, on a cellular level (ex vivo). Cell survival rate of HepG2.2.15 cells was detected by MTT assay. HBsAg and HBeAg in HepG 2.2.15 cell supernatant were evaluated by enzyme linked immunosorbent assay (ELISA). Results showed that water extract from Radix Trichosanthis had a stronger inhibitive effect on expression of HBsAg and HBeAg in HepG2.2.15 cells than the alcohol extract from the same plant. Considering that the most active component of Radix Trichosanthis was in its aqueous extract and this might be related to the active component Trichosanthin. Trichosanthin was further used for related experiments to confirm this hypothesis. The results showed that Trichosanthin, in the aqueous extract from Radix Trichosanthis, is likely the main component responsible for the anti-hepatitis B viral effect.
authors.forename: Doudou
authors.initials: D
authors.lastname: Wen
authors.forename: Jianing
authors.initials: J
authors.lastname: Wang
authors.forename: Huiwen
authors.initials: H
authors.lastname: Yan
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.forename: Kun
authors.initials: K
authors.lastname: Xia
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Ailian
authors.initials: A
authors.lastname: Zhang
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D056945:Hep G2 Cells; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D015978:Trichosanthin / Q000494:pharmacology*
chemical_list: D000998:Antiviral Agents; D017077:Culture Media, Conditioned; D004365:Drugs, Chinese Herbal; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; C034634:radix Trichosanthis; D015978:Trichosanthin