title: Proteomics of the organohalide-respiring Epsilonproteobacterium Sulfurospirillum multivorans adapted to tetrachloroethene and other energy substrates.
abstract: Organohalide respiration is an environmentally important but poorly characterized type of anaerobic respiration. We compared the global proteome of the versatile organohalide-respiring Epsilonproteobacterium Sulfurospirillum multivorans grown with different electron acceptors (fumarate, nitrate, or tetrachloroethene [PCE]). The most significant differences in protein abundance were found for gene products of the organohalide respiration region. This genomic region encodes the corrinoid and FeS cluster containing PCE reductive dehalogenase PceA and other proteins putatively involved in PCE metabolism such as those involved in corrinoid biosynthesis. The latter gene products as well as PceA and a putative quinol dehydrogenase were almost exclusively detected in cells grown with PCE. This finding suggests an electron flow from the electron donor such as formate or pyruvate via the quinone pool and a quinol dehydrogenase to PceA and the terminal electron acceptor PCE. Two putative accessory proteins, an IscU-like protein and a peroxidase-like protein, were detected with PCE only and might be involved in PceA maturation. The proteome of cells grown with pyruvate instead of formate as electron donor indicates a route of electrons from reduced ferredoxin via an Epsilonproteobacterial complex I and the quinone pool to PCE.
authors.affiliation: Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, 07743 Jena, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Goris
authors.affiliation: Department of Proteomics, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, Germany.
authors.forename: Christian L
authors.initials: CL
authors.lastname: Schiffmann
authors.affiliation: Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, 07743 Jena, Germany.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Gadkari
authors.affiliation: Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, 07743 Jena, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Schubert
authors.affiliation: Institute of Animal Science, Hohenheim University, 70593 Stuttgart, Germany.
authors.forename: Jana
authors.initials: J
authors.lastname: Seifert
authors.affiliation: Department of Proteomics, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, Germany.
authors.forename: Nico
authors.initials: N
authors.lastname: Jehmlich
authors.affiliation: Department of Proteomics, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, Germany.|Aalborg University, Department of Biotechnology, Chemistry and Environmental Engineering, Sohngårdsholmsvej 49, 9000 Aalborg, Denmark.|Department of Metabolomics, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: von Bergen
authors.affiliation: Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, 07743 Jena, Germany.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Diekert
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004734:Energy Metabolism / Q000235:genetics / Q000502:physiology*; D020565:Epsilonproteobacteria / Q000235:genetics / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D020869:Gene Expression Profiling; D009566:Nitrates / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010101:Oxygen Consumption; D025341:Principal Component Analysis; D040901:Proteomics; D019289:Pyruvic Acid / Q000378:metabolism; D011809:Quinones / Q000378:metabolism; D013750:Tetrachloroethylene / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D005288:Ferredoxins; D005561:Formates; D009566:Nitrates; D011809:Quinones; C030544:formic acid; D019289:Pyruvic Acid; D010088:Oxidoreductases; C112990:tetrachloroethene dehalogenase; C098849:tetrachloroethene reductase; D013750:Tetrachloroethylene