title: Chaxapeptin, a Lasso Peptide from Extremotolerant Streptomyces leeuwenhoekii Strain C58 from the Hyperarid Atacama Desert.
abstract: Lasso peptides are ribosomally synthesized and post-translationally modified peptides (RiPPs) that possess a unique "lariat knot" structural motif. Genome mining-targeted discovery of new natural products from microbes obtained from extreme environments has led to the identification of a gene cluster directing the biosynthesis of a new lasso peptide, designated as chaxapeptin 1, in the genome of Streptomyces leeuwenhoekii strain C58 isolated from the Atacama Desert. Subsequently, 1 was isolated and characterized using high-resolution electrospray ionization mass spectrometry and nuclear magnetic resonance methods. The lasso nature of 1 was confirmed by calculating its nuclear Overhauser effect restraint-based solution structure. Chaxapeptin 1 displayed a significant inhibitory activity in a cell invasion assay with human lung cancer cell line A549.
authors.affiliation: Marine Biodiscovery Centre, University of Aberdeen , Meston Walk, Aberdeen AB24 3UE, Scotland, U.K.
authors.forename: Somayah S
authors.initials: SS
authors.lastname: Elsayed
authors.affiliation: Aberdeen Oomycetes Laboratory, Institute of Medical Sciences, University of Aberdeen , Aberdeen AB25 2ZD, Scotland, U.K.
authors.forename: Franziska
authors.initials: F
authors.lastname: Trusch
authors.affiliation: Marine Biodiscovery Centre, University of Aberdeen , Meston Walk, Aberdeen AB24 3UE, Scotland, U.K.
authors.forename: Hai
authors.initials: H
authors.lastname: Deng
authors.affiliation: Marine Biodiscovery Centre, University of Aberdeen , Meston Walk, Aberdeen AB24 3UE, Scotland, U.K.
authors.forename: Andrea
authors.initials: A
authors.lastname: Raab
authors.affiliation: Department of Chemistry, University of Warwick , Coventry CV4 7AL, U.K.
authors.forename: Ivan
authors.initials: I
authors.lastname: Prokes
authors.affiliation: School of Biology, Newcastle University , Newcastle upon Tyne NE1 7RU, U.K.
authors.forename: Kanungnid
authors.initials: K
authors.lastname: Busarakam
authors.affiliation: Centre for Biotechnology and Bioengineering, CeBiB, University of Chile , Beauchef 850, Santiago, Chile.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Asenjo
authors.affiliation: Centre for Biotechnology and Bioengineering, CeBiB, University of Chile , Beauchef 850, Santiago, Chile.
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Andrews
authors.affiliation: Aberdeen Oomycetes Laboratory, Institute of Medical Sciences, University of Aberdeen , Aberdeen AB25 2ZD, Scotland, U.K.
authors.forename: Pieter
authors.initials: P
authors.lastname: van West
authors.affiliation: School of Biosciences, University of Kent , Canterbury, Kent CT2 7NJ, U.K.
authors.forename: Alan T
authors.initials: AT
authors.lastname: Bull
authors.affiliation: School of Biology, Newcastle University , Newcastle upon Tyne NE1 7RU, U.K.
authors.forename: Michael
authors.initials: M
authors.lastname: Goodfellow
authors.affiliation: Key Laboratory of Combinatory Biosynthesis and Drug Discovery, School of Pharmaceutical Sciences, Wuhan University , 185 East Lake Road, Wuhan 430071, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yi
authors.affiliation: Marine Biodiscovery Centre, University of Aberdeen , Meston Walk, Aberdeen AB24 3UE, Scotland, U.K.
authors.forename: Rainer
authors.initials: R
authors.lastname: Ebel
authors.affiliation: Marine Biodiscovery Centre, University of Aberdeen , Meston Walk, Aberdeen AB24 3UE, Scotland, U.K.
authors.forename: Marcel
authors.initials: M
authors.lastname: Jaspars
authors.affiliation: Marine Biodiscovery Centre, University of Aberdeen , Meston Walk, Aberdeen AB24 3UE, Scotland, U.K.|Pharmacognosy Department, Faculty of Pharmacy, Beni-Suef University , Beni-Suef 32514, Egypt.
authors.forename: Mostafa E
authors.initials: ME
authors.lastname: Rateb
mesh_terms: D000595:Amino Acid Sequence; D001688:Biological Products / Q000737:chemistry*; D002460:Cell Line / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism; D018942:Macrolides / Q000737:chemistry* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D012270:Ribosomes / Q000737:chemistry*; D013302:Streptomyces / Q000737:chemistry*
chemical_list: D001688:Biological Products; D018942:Macrolides; D010455:Peptides; D010456:Peptides, Cyclic; C567950:chaxalactin A; C000606229:chaxapeptin