title: Short-chain fatty acids produced by synbiotic mixtures in skim milk differentially regulate proliferation and cytokine production in peripheral blood mononuclear cells.
abstract: Short chain fatty acids (SCFAs) are major products of prebiotic fermentation and confer human health benefits such as immune-regulation. In this study, reconstituted skim milk supplemented with prebiotics (RSMP) including inulin, hi-maize or β-glucan was fermented by probiotic strains of Lactobacillus spp. and Bifidobacteria spp. After 24 h of fermentation, probiotics growth and SCFAs production were investigated and the produced SCFAs were extracted. Inulin and Lactobacillus rhamnosus GG ATCC 53013 (LGG) combination released highest concentrations of SCFAs compared to LGG and hi-maize or β-glucan. Extracted SCFAs were then used for in vitro immune modulation study in human peripheral blood mononuclear cells (PBMCs). In lipopolysaccharide (LPS)-stimulated PBMCs, SCFAs particularly butyrate down-regulated tumor necrosis factor alpha, interleukin (IL)-12, interferon gamma (IFN-γ) and transforming growth factor beta-1 (TGF-β1), and up-regulated IL-4, IL-10, while no significant effect was noted in non-LPS-stimulated PBMCs. The results indicate that SCFAs regulated cytokine milieu in LPS-stimulated PBMCs to anti-inflammatory cytokines.
authors.affiliation: a Advanced Food Systems Research Unit and.
authors.forename: M
authors.initials: M
authors.lastname: Asarat
authors.affiliation: b Center of Chronic Disease Prevention and Management, College of Health and Biomedicine, Victoria University , Melbourne , VIC , Australia.
authors.forename: V
authors.initials: V
authors.lastname: Apostolopoulos
authors.affiliation: a Advanced Food Systems Research Unit and.
authors.forename: T
authors.initials: T
authors.lastname: Vasiljevic
authors.affiliation: a Advanced Food Systems Research Unit and.
authors.forename: O
authors.initials: O
authors.lastname: Donkor
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000378:metabolism; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000096:biosynthesis / Q000494:pharmacology* / Q000627:therapeutic use; D005285:Fermentation; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism*; D007444:Inulin / Q000378:metabolism; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008070:Lipopolysaccharides; D008892:Milk; D056692:Prebiotics; D019936:Probiotics; D058616:Synbiotics; D003313:Zea mays; D047071:beta-Glucans / Q000378:metabolism
chemical_list: D016207:Cytokines; D005232:Fatty Acids, Volatile; D008070:Lipopolysaccharides; D056692:Prebiotics; D047071:beta-Glucans; D007444:Inulin
keywords: Cytokines; fermentation; peripheral blood mononuclear cells; probiotics; short chain fatty acids