title: Structural Reorganization and Fibrinogen Adsorption Behaviors on the Polyrotaxane Surfaces Investigated by Sum Frequency Generation Spectroscopy.
abstract: Polyrotaxanes, such as supramolecular assemblies with methylated α-cyclodextrins (α-CDs) as host molecules noncovalently threaded on the linear polymer backbone, are promising materials for biomedical applications because they allow adsorbed proteins possessing a high surface flexibility as well as control of the cellular morphology and adhesion. To provide a general design principle for biomedical materials, we examined the surface reorganization behaviors and adsorption conformations of fibrinogen on the polyrotaxane surfaces with comparison to several random copolymers by sum frequency generation (SFG) vibrational spectroscopy. We showed that the polyrotaxane (OMe-PRX-PMB) with methylated α-CDs as the host molecule exhibited unique surface structures in an aqueous environment. The hydrophobic interaction between the methoxy groups of the methylated α-CD molecules and methyl groups of the n-butyl methacrylate (BMA) side chains may dominate the surface restructuring behavior of the OMe-PRX-PMB. The orientation analysis revealed that the orientation of the fibrinogen adsorbed on the OMe-PRX-PMB surface is close to a single distribution, which is different from the adsorption behaviors of fibrinogen on other polyrotaxane or random copolymer surfaces.
authors.affiliation: Catalysis Research Center, Hokkaido University , Sapporo 001-0021, Japan.
authors.forename: Aimin
authors.initials: A
authors.lastname: Ge
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University , Tokyo 101-0062, Japan.
authors.forename: Ji-Hun
authors.initials: JH
authors.lastname: Seo
authors.affiliation: Catalysis Research Center, Hokkaido University , Sapporo 001-0021, Japan.
authors.forename: Lin
authors.initials: L
authors.lastname: Qiao
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University , Tokyo 101-0062, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Yui
authors.affiliation: Catalysis Research Center, Hokkaido University , Sapporo 001-0021, Japan.
authors.forename: Shen
authors.initials: S
authors.lastname: Ye
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry; D003505:Cyclodextrins / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry* / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D020442:Poloxamer / Q000737:chemistry*; D043862:Rotaxanes / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D003505:Cyclodextrins; D043862:Rotaxanes; C475673:polyrotaxane; D014867:Water; D020442:Poloxamer; D005340:Fibrinogen
keywords: SFG vibrational spectroscopy; biomedical materials; fibrinogen adsorption; polyrotaxane; surface reorganization; α-cyclodextrin (α-CD)