title: Inhibition of squamous cancer growth in a mouse model by Staphylococcal enterotoxin B-triggered Th9 cell expansion.
abstract: Currently, therapy for squamous cancer (SqC) is unsatisfactory. Staphylococcal enterotoxin B (SEB) has strong immune regulatory activity. This study tests the hypothesis that SEB enforces the effect of immunotherapy on SqC growth in a mouse model. C3H/HeN mice and the SqC cell line squamous cell carcinoma VII were used to create an SqC mouse model. Immune cell assessment was performed by flow cytometry. Real-time RT-PCR and western blotting were used to evaluate target molecule expression. An apoptosis assay was used to assess the suppressive effect of T helper-9 (Th9) cells on the SqC cells. The results showed that immunotherapy consisting of SEB plus SqC antigen significantly inhibited SqC growth in the mice. The frequency of Th9 cells was markedly increased in the SqC tissue and mouse spleens after treatment. SEB markedly increased the levels of signal transducer and activator of transcription 5 phosphorylation and the expression of histone deacetylase-1 (HDAC1) and PU.1 (the transcription factor of the interleukin 9 (IL-9) gene) in CD4+T cells. Exposure to SqC-specific Th9 cells markedly induced SqC cell apoptosis both in vitro and in vivo. In conclusion, the administration of SEB induces Th9 cells in SqC-bearing mice, and theseTh9 cells inhibit SqC growth.
authors.affiliation: Department of Otolaryngology, the First Affiliated Hospital of Shenzhen University and the Second People's Hospital of Shenzhen, Shenzhen, China.|Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors.forename: Bei-Ping
authors.initials: BP
authors.lastname: Miao
authors.affiliation: Department of Otolaryngology, the First Affiliated Hospital of Shenzhen University and the Second People's Hospital of Shenzhen, Shenzhen, China.
authors.forename: Rui-Shi
authors.initials: RS
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, the First Affiliated Hospital of Shenzhen University and the Second People's Hospital of Shenzhen, Shenzhen, China.
authors.forename: Huan-Ji
authors.initials: HJ
authors.lastname: Sun
authors.affiliation: Department of Otolaryngology, the First Affiliated Hospital of Shenzhen University and the Second People's Hospital of Shenzhen, Shenzhen, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Yu
authors.affiliation: Department of Otolaryngology, the First Affiliated Hospital of Shenzhen University and the Second People's Hospital of Shenzhen, Shenzhen, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Brain Body Institute, McMaster University, Hamilton, ON L8N 4A6, Canada.
authors.forename: Lin-Jing
authors.initials: LJ
authors.lastname: Li
authors.affiliation: Brain Body Institute, McMaster University, Hamilton, ON L8N 4A6, Canada.
authors.forename: Jiang-Qi
authors.initials: JQ
authors.lastname: Liu
authors.affiliation: Shenzhen Women & Children Healthcare, Shenzhen, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors.forename: Hai-Qiong
authors.initials: HQ
authors.lastname: Yu
authors.affiliation: Department of Respirology, the First Affiliated Hospital of Shenzhen University and the Second People's Hospital of Shenzhen, Shenzhen, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Liu
authors.affiliation: Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors.forename: Ping-Chang
authors.initials: PC
authors.lastname: Yang
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D004768:Enterotoxins / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D016906:Interleukin-9 / Q000097:blood; D051379:Mice; D018307:Neoplasms, Squamous Cell / Q000097:blood / Q000276:immunology* / Q000473:pathology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D000951:Antigens, Neoplasm; D004768:Enterotoxins; D016906:Interleukin-9; C031818:enterotoxin B, staphylococcal