title: Host-Guest Interaction-Based Self-Engineering of Nano-Sized Vesicles for Co-Delivery of Genes and Anticancer Drugs.
abstract: On the basis of host-guest interactions, this study reported a kind of linear-hyperbranched supramolecular amphiphile and its assembled vesicles for the combined achievement of drug encapsulation and DNA delivery. Amine-attached β-cyclodextrin-centered hyperbranched polyglycerol and linear adamantane-terminated octadecane were arranged to spontaneously interlink together and then self-assemble into nanoscale vesicles. As the model of a hydrophilic agent, DOX·HCl was demonstrated to be readily loaded into the hollow cavity of the vesicles. The drug release pattern could be controlled by adjusting the environmental acidity, favoring the intracellularly fast drug liberation in response to the cellular lysosomal microenvironment. The nanovesicles displayed superior serum-tolerant transgene ability and significantly lower cytotoxicity compared to those of PEI25K, the gold standard of gene delivery vectors. The drug-loaded nanovesicle can co-deliver DNA payloads into cells and allow the preferable accumulation of two payloads in nuclei. The drug encapsulation was found to have little influence on the transfection. This co-delivery vehicle presents a good example of rational design of cationic supramolecular vesicles for stimulus-responsive drug/DNA transport.
authors.affiliation: Key Laboratory of Biomedical Polymers (Ministry of Education), Department of Chemistry, Wuhan University , Wuhan 430072, P. R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Key Laboratory of Biomedical Polymers (Ministry of Education), Department of Chemistry, Wuhan University , Wuhan 430072, P. R. China.
authors.forename: Xing
authors.initials: X
authors.lastname: Dong
authors.affiliation: Key Laboratory of Biomedical Polymers (Ministry of Education), Department of Chemistry, Wuhan University , Wuhan 430072, P. R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lei
authors.affiliation: Key Laboratory of Biomedical Polymers (Ministry of Education), Department of Chemistry, Wuhan University , Wuhan 430072, P. R. China.
authors.forename: Renxi
authors.initials: R
authors.lastname: Zhuo
authors.affiliation: Key Laboratory of Biomedical Polymers (Ministry of Education), Department of Chemistry, Wuhan University , Wuhan 430072, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Feng
authors.affiliation: Key Laboratory of Biomedical Polymers (Ministry of Education), Department of Chemistry, Wuhan University , Wuhan 430072, P. R. China.
authors.forename: Xianzheng
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000493:pharmacokinetics; D019556:COS Cells; D002522:Chlorocebus aethiops; D004247:DNA / Q000737:chemistry* / Q000493:pharmacokinetics; D018014:Gene Transfer Techniques; D006801:Humans; D008081:Liposomes; D061986:MCF-7 Cells; D018613:Microscopy, Confocal; D050997:Nanomedicine / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D004247:DNA
keywords: drug encapsulation; gene delivery; host−guest interaction; nanovesicles; supramolecular assembly