title: A Novel Technique for Amniotic Membrane Transplantation in Patients with Acute Stevens-Johnson Syndrome.
abstract: Cryopreserved amniotic membrane (AM) transplantation is an emerging technique that is becoming the gold standard for the management of acute Stevens-Johnson syndrome (SJS) and its more severe variant, toxic epidermal necrolysis (TEN). We describe a novel surgical technique utilizing a single, large sheet of AM (5 x 10 cm) and a custom-made forniceal ring, which facilitates AM placement. Our technique is easy to use and minimizes suturing and manipulation of ocular tissues, resulting in decreased operative time. This technique may be applied in the management of multiple ocular surface disease processes, including chemical or thermal burns, severe ocular graft versus host disease (GVHD), and other autoimmune diseases.
authors.affiliation: Department of Ophthalmology, Boston University Medical Center, Boston University School of Medicine, Boston, MA, USA.
authors.forename: Kelly N
authors.initials: KN
authors.lastname: Ma
authors.affiliation: Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA, USA.
authors.forename: Aristomenis
authors.initials: A
authors.lastname: Thanos
authors.affiliation: Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA, USA.
authors.forename: James
authors.initials: J
authors.lastname: Chodosh
authors.affiliation: Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA, USA; Department of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.
authors.forename: Ankoor S
authors.initials: AS
authors.lastname: Shah
authors.affiliation: Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA, USA; Department of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA. Electronic address: jason.mantagos@childrens.harvard.edu.
authors.forename: Iason S
authors.initials: IS
authors.lastname: Mantagos
mesh_terms: D000208:Acute Disease; D000650:Amnion / Q000637:transplantation*; D006801:Humans; D013508:Ophthalmologic Surgical Procedures / Q000379:methods*; D013262:Stevens-Johnson Syndrome / Q000601:surgery*
keywords: Stevens-Johnson syndrome; amniotic membrane; cryopreserved amniotic membrane; ocular surface; transplantation