title: LED fluorescence microscopy increases the detection of smear-positive pulmonary tuberculosis in medical colleges of India.
abstract: In July 2012, light-emitting diode fluorescence microscopy (LED-FM) replaced conventional light microscopy using Ziehl-Neelsen stain in the detection of sputum-positive pulmonary tuberculosis in 190 microscopy centres of medical colleges operating under India's Revised National Tuberculosis Control Programme. We compared the performance of LED-FM (July-December 2012) to that of conventional microscopy (July-December 2011) across 190 sites. Of 222 658 patients examined using conventional microscopy, 28 042 (12.6%) were smear-positive, while of 224 714 examined using LED-FM, 33 552 (14.9%) were smear-positive, an additional yield of 5251 cases after adjusting for the increase in patients examined. We recommend replacing conventional microscopy with LED-FM in high workload microscopy centres in India.
authors.affiliation: International Union Against Tuberculosis and Lung Disease, South-East Asia Office, New Delhi, India.
authors.forename: L W
authors.initials: LW
authors.lastname: Reza
authors.affiliation: International Union Against Tuberculosis and Lung Disease, South-East Asia Office, New Delhi, India.
authors.forename: S
authors.initials: S
authors.lastname: Satyanarayana
authors.affiliation: International Union Against Tuberculosis and Lung Disease, South-East Asia Office, New Delhi, India.
authors.forename: A
authors.initials: A
authors.lastname: Pandey
authors.affiliation: International Union Against Tuberculosis and Lung Disease, South-East Asia Office, New Delhi, India.
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.affiliation: International Union Against Tuberculosis and Lung Disease, South-East Asia Office, New Delhi, India.
authors.forename: N M
authors.initials: NM
authors.lastname: Devendrappa
authors.affiliation: International Union Against Tuberculosis and Lung Disease, South-East Asia Office, New Delhi, India.
authors.forename: L
authors.initials: L
authors.lastname: Anand
authors.affiliation: International Union Against Tuberculosis and Lung Disease, South-East Asia Office, New Delhi, India.
authors.forename: G
authors.initials: G
authors.lastname: Singh
authors.affiliation: International Union Against Tuberculosis and Lung Disease, South-East Asia Office, New Delhi, India.
authors.forename: A M V
authors.initials: AM
authors.lastname: Kumar
authors.affiliation: International Union Against Tuberculosis and Lung Disease, South-East Asia Office, New Delhi, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Chadha
authors.affiliation: International Union Against Tuberculosis and Lung Disease, South-East Asia Office, New Delhi, India.
authors.forename: N
authors.initials: N
authors.lastname: Wilson
authors.affiliation: Central TB Division, Directorate General of Health Services, Ministry of Health and Family Welfare, Government of India, New Delhi, India.
authors.forename: K S
authors.initials: KS
authors.lastname: Sachdeva
authors.affiliation: World Health Organization Country Office for India, New Delhi, India.
authors.forename: S A
authors.initials: SA
authors.lastname: Nair
keywords: India; LED-FM; fluorescence microscopy; medical colleges; tuberculosis