title: Stromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic rats.
abstract: BACKGROUND
  Adipose-derived stem cells (ADSCs) were effective in treating wound. Stromal cell-derived factor-1 (SDF-1), a chemokine usually called CXCL12, is well known for its chemotaxis in induction of cell migration. However, little is known about the SDF-1responsible for the complex migration of ADSCs from residence to injured sites.
  
  
  OBJECTIVE
  Herein, we firstly showed SDF-1 is a major regulator involved in migration of ADSCs during wound repair in vivo.
  
  
  METHODS
  Trauma in rats was induced by surgical operation. The levels of SDF-1 in wounded tissue were assayed by ELISA. ADSCs were labeled with Green Fluorescent Protein (GFP), and then were transferred to injured rats by intracarotid injection. The plasma levels of ADSCs during wound healing were detected by flow cytometry, and ADSCs in injured tissue were evaluated by bioluminescence imaging in vivo and laser confocal microscopy (LCM), respectively.
  
  
  RESULTS
  ADSCs were successfully labeled with GFP. SDF-1 level reached to the peak value on 24 h after injury and then decreased continuously. Additionally, levels of plasma ADSCs in SDF-1 treated rats reached to the peak value (12%) at d21 after medicine delivery, while those of normal and injured rats showed the peak values of 6.28% and 9.84% at d7 and d21, respectively. Finally, the results of LCM indicated treatment of ectogenic SDF-1 obviously enhanced GFP-ADSCs distribution in wounded tissues.
  
  
  CONCLUSIONS
  Our results indicated that SDF-1 treatment obviously promoted the migration and directed distribution of ADSCs in traumatic tissue.
authors.affiliation: Department of Plastic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Plastic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China.
authors.forename: Fu-Kang
authors.initials: FK
authors.lastname: Ji
authors.affiliation: Department of Plastic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China.
authors.forename: Jin-Huang
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Department of Plastic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Nan
authors.affiliation: Department of Plastic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China. Electronic address: liudalieedu@sina.com.
authors.forename: Da-Lie
authors.initials: DL
authors.lastname: Liu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D054377:Chemokine CXCL12 / Q000494:pharmacology / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
chemical_list: C575935:CXCL12 protein, rat; D054377:Chemokine CXCL12; D049452:Green Fluorescent Proteins
keywords: Adipose-derived stem cells; Intracarotid injection; Stromal cell-derived factor 1; Wound