title: [The alterations of apoptosis factor Bcl-2/Bax in the early Parkinson's disease rats and the protective effect of scorpion venom derived activity peptide].
abstract: OBJECTIVE
  To explore the alterations of apoptosis factor Bcl-2/Bax in the early Parkinson's disease (PD) rats and the protective effect of scorpion venom derived bioactive peptide.
  
  
  METHODS
  Healthy male SD rats (180-220 g) were randomly divided into 4 groups (n = 10): early PD model group, sham operation group, scorpion venom derived bioactive peptide control group, scorpion venom derived bioactive peptide therapy group. 6-hydroxydopamine (6-OHDA) was used to prepare the early PD rat model. The immunohistochemistry was used to detect the expression of Bax and Bcl-2 and further explore the mechanism of anti-apoptosis regarding the neuroprotective effect of scorpion venom derived bioactive peptide.
  
  
  RESULTS
  The results indicated that compared with the control rats, the immunostaining of Bax in the brain increased significantly while that of Bcl-2 decreased significantly in the lesion side of 6-OHDA treated rats. Interestingly, scorpion venom derived bioactive peptide could attenuate the above abnormal changes.
  
  
  CONCLUSIONS
  Up-regulation of Bax and down-regulation of Bcl-2 could participate in the early stage of PD and the anti-apoptotic mechanism could be involved in the neuroprotective effect exerted by scorpion venom derived activity peptide regarding the dopaminergic neuron in the early stage.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Dong
authors.initials: D
authors.lastname: An
authors.forename: Sheng-ming
authors.initials: SM
authors.lastname: Yin
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: Xu
authors.initials: X
authors.lastname: Meng
authors.forename: De-qin
authors.initials: DQ
authors.lastname: Yu
authors.forename: Yi-ping
authors.initials: YP
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Wan-qin
authors.initials: WQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D004195:Disease Models, Animal; D015536:Down-Regulation; D008297:Male; D018696:Neuroprotective Agents / Q000737:chemistry*; D016627:Oxidopamine; D010300:Parkinson Disease / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012604:Scorpion Venoms / Q000737:chemistry*; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
chemical_list: C494828:Bax protein, rat; D018696:Neuroprotective Agents; D010455:Peptides; D019253:Proto-Oncogene Proteins c-bcl-2; D012604:Scorpion Venoms; D051028:bcl-2-Associated X Protein; D016627:Oxidopamine