title: Harvesting (67)Cu from the Collection of a Secondary Beam Cocktail at the National Superconducting Cyclotron Laboratory.
abstract: Isotope harvesting is a promising new method to obtain isotopes for which there is no reliable continuous supply at present. To determine the possibility of obtaining radiochemically pure radioisotopes from an aqueous beam dump at a heavy-ion fragmentation facility, preliminary experiments were performed to chemically extract a copper isotope from a large mixture of projectile fragmentation products in an aqueous medium. In this work a 93 MeV/u secondary beam cocktail was collected in an aqueous beam stop at the National Superconducting Cyclotron Laboratory (NSCL) located on the Michigan State University (MSU) campus. The beam cocktail consisted of ∼2.9% (67)Cu in a large mixture of co-produced isotopes ranging in atomic number from ∼19 to 34. The chemical extraction of (67)Cu was achieved via a two-step process: primary extraction using a divalent metal chelation disk followed by anion-exchange chromatography. A significant fraction (74 ± 4%) of the (67)Cu collected in the aqueous beam stop was recovered with >99% radiochemical purity. To illustrate the utility of this product, the purified (67)Cu material was then used to radiolabel an anti-EGFR antibody, Panitumumab, and injected into mice bearing colon cancer xenografts. The tumor uptake at 5 days postinjection was found to be 12.5 ± 0.7% which was in very good agreement with previously reported studies with this radiolabeled antibody. The present results demonstrate that harvesting isotopes from a heavy-ion fragmentation facility could be a promising new method for obtaining high-quality isotopes that are not currently available by traditional methods.
authors.affiliation: Department of Chemistry, Washington University in St. Louis , One Brookings Drive, St. Louis, Missouri 63139, United States.|Department of Radiology, Washington University School of Medicine , 510 S. Kingshighway Boulevard, St. Louis, Missouri 63110, United States.
authors.forename: Tara
authors.initials: T
authors.lastname: Mastren
authors.affiliation: Department of Chemistry, Hope College , 35 East 12th Street, Holland, Michigan 49422, United States.
authors.forename: Aranh
authors.initials: A
authors.lastname: Pen
authors.affiliation: Department of Radiology, Washington University School of Medicine , 510 S. Kingshighway Boulevard, St. Louis, Missouri 63110, United States.
authors.forename: Shaun
authors.initials: S
authors.lastname: Loveless
authors.affiliation: Department of Radiology, Washington University School of Medicine , 510 S. Kingshighway Boulevard, St. Louis, Missouri 63110, United States.
authors.forename: Bernadette V
authors.initials: BV
authors.lastname: Marquez
authors.affiliation: Department of Radiology, Washington University School of Medicine , 510 S. Kingshighway Boulevard, St. Louis, Missouri 63110, United States.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Bollinger
authors.affiliation: Department of Chemistry, Hope College , 35 East 12th Street, Holland, Michigan 49422, United States.
authors.forename: Boone
authors.initials: B
authors.lastname: Marois
authors.affiliation: Department of Chemistry, Hope College , 35 East 12th Street, Holland, Michigan 49422, United States.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Hubley
authors.affiliation: Department of Chemistry, Washington University in St. Louis , One Brookings Drive, St. Louis, Missouri 63139, United States.
authors.forename: Kyle
authors.initials: K
authors.lastname: Brown
authors.affiliation: Department of Chemistry, Michigan State University , 578 South Shaw Lane, East Lansing, Michigan 48824, United States.
authors.forename: David J
authors.initials: DJ
authors.lastname: Morrissey
authors.affiliation: Department of Chemistry, Hope College , 35 East 12th Street, Holland, Michigan 49422, United States.
authors.forename: Graham F
authors.initials: GF
authors.lastname: Peaslee
authors.affiliation: Department of Chemistry, Washington University in St. Louis , One Brookings Drive, St. Louis, Missouri 63139, United States.|Department of Radiology, Washington University School of Medicine , 510 S. Kingshighway Boulevard, St. Louis, Missouri 63110, United States.
authors.forename: Suzanne E
authors.initials: SE
authors.lastname: Lapi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism / Q000532:radiotherapy; D003301:Copper Radioisotopes / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification* / Q000493:pharmacokinetics; D017357:Cyclotrons; D005260:Female; D006801:Humans; D007753:Laboratories; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000532:radiotherapy; D000077544:Panitumumab; D019275:Radiopharmaceuticals / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D003301:Copper Radioisotopes; D019275:Radiopharmaceuticals; D000077544:Panitumumab