title: The tumor-suppressive function of miR-1 by targeting LASP1 and TAGLN2 in esophageal squamous cell carcinoma.
abstract: OBJECTIVE
  This study determined the expression of microRNA-1 in esophageal squamous cell carcinoma (ESCC) tissue and cell lines to evaluate its effects on clinicopathological parameters and its target genes LASP1 and TAGLN2.
  
  
  METHODS
  The expression of miR-1, lasp1, and tagln2 was detected in 55 ESCC tissues and adjacent normal tissues by reverse transcription-polymerase chain reaction (RT-PCR). The association between miR-1, lasp1, and tagln2 expression and clinicopathological characteristics was observed. MicroRNA-1 (mimics-miR-1) and its inhibitor (Inhibitor-miR-1) were transfected into esophageal cancer cells KYSE 510 and Eca 109; cell proliferation, migration, and invasion assays were carried out. Plasmid construction and dual-luciferase reporter assay were also carried out to indicate whether LASP1 and TAGLN2 were miR-1 target genes. The expression of LASP1 and TAGLN2 was detected with Western blot methods in cell lines, by immunohistochemistry in ESCC tissue.
  
  
  RESULTS
  The gene expression level of microRNA-1 in cancer tissues was significantly lower than that in adjacent normal tissues (P < 0.01). The expression of miR-1 in ESCC was correlated with involvement of lymph nodes (P = 0.002), histologic classification (P = 0.000), and vessel invasion (P = 0.022). The expression of lasp1 and tagln2 increased in cancer tissues compared with in adjacent normal tissues (P < 0.05). MiR-1 suppresses the cell growth, migration, and invasion in vitro. The expression of LASP1 and TAGLN2 decreased in mimics-miR-1 transfected cells, and increased in inhibitor-miR-1 transfected cells. Luciferase reporter assay confirmed that LASP1 and TAGLN2 mRNA actually had the target sites of miR-1.
  
  
  CONCLUSIONS
  miR-1 suppresses cell proliferation, invasiveness, metastasis, and progression of ESCC by binding its targeted genes LASP1 and TAGLN2.
authors.affiliation: Department of Clinical Laboratory, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Du
authors.affiliation: Scientific Research Center, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Lian-Mei
authors.initials: LM
authors.lastname: Zhao
authors.affiliation: Scientific Research Center, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Scientific Research Center, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Mei-Xiang
authors.initials: MX
authors.lastname: Sang
authors.affiliation: Scientific Research Center, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Scientific Research Center, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Ma
authors.affiliation: Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Liu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism; D018450:Disease Progression; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D018390:Gene Targeting; D006801:Humans; D060588:LIM Domain Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis / Q000235:genetics; D011485:Protein Binding; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; C096353:LASP1 protein, human; D060588:LIM Domain Proteins; C544547:MIRN1 microRNA, human; D035683:MicroRNAs; D008840:Microfilament Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; C585387:Tagln2 protein, human
keywords: LASP1; TAGLN2; esophageal squamous cell carcinoma; microRNA-1