title: Role of Homothorax in region specific regulation of Deformed in embryonic neuroblasts.
abstract: The expression and regulation of Hox genes in developing central nervous system (CNS) lack important details like specific cell types where Hox genes are expressed and the transcriptional regulatory players involved in these cells. In this study we have investigated the expression and regulation of Drosophila Hox gene Deformed (Dfd) in specific cell types of embryonic CNS. Using Dfd neural autoregulatory enhancer we find that Dfd autoregulates itself in cells of mandibular neuromere. We have also investigated the role of a Hox cofactor Homothorax (Hth) for its role in regulating Dfd expression in CNS. We find that Hth exhibits a region specific role in controlling the expression of Dfd, but has no direct role in mandibular Dfd neural autoregulatory circuit. Our results also suggest that homeodomain of Hth is not required for regulating Dfd expression in embryonic CNS.
authors.affiliation: Laboratory of Drosophila Neural Development, Centre for DNA Fingerprinting and Diagnostics (CDFD), 4-1-714, Tuljaguda Complex, Nampally, Hyderabad-500001, India; Graduate Studies, Manipal University, Manipal 576104, India.
authors.forename: Raviranjan
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Laboratory of Drosophila Neural Development, Centre for DNA Fingerprinting and Diagnostics (CDFD), 4-1-714, Tuljaguda Complex, Nampally, Hyderabad-500001, India.
authors.forename: Maheshvari
authors.initials: M
authors.lastname: Chotaliya
authors.affiliation: Laboratory of Drosophila Neural Development, Centre for DNA Fingerprinting and Diagnostics (CDFD), 4-1-714, Tuljaguda Complex, Nampally, Hyderabad-500001, India.
authors.forename: Sruthakeerthi
authors.initials: S
authors.lastname: Vuppala
authors.affiliation: Laboratory of Drosophila Neural Development, Centre for DNA Fingerprinting and Diagnostics (CDFD), 4-1-714, Tuljaguda Complex, Nampally, Hyderabad-500001, India.
authors.forename: Ankush
authors.initials: A
authors.lastname: Auradkar
authors.affiliation: Laboratory of Drosophila Neural Development, Centre for DNA Fingerprinting and Diagnostics (CDFD), 4-1-714, Tuljaguda Complex, Nampally, Hyderabad-500001, India.
authors.forename: Kalyani
authors.initials: K
authors.lastname: Palasamudrum
authors.affiliation: Laboratory of Drosophila Neural Development, Centre for DNA Fingerprinting and Diagnostics (CDFD), 4-1-714, Tuljaguda Complex, Nampally, Hyderabad-500001, India. Electronic address: rohit@cdfd.org.in.
authors.forename: Rohit
authors.initials: R
authors.lastname: Joshi
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000196:embryology*; D004330:Drosophila / Q000196:embryology / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics; D004742:Enhancer Elements, Genetic / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D005801:Genes, Homeobox / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics*; D058953:Neural Stem Cells / Q000502:physiology*; D038081:Organogenesis / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D018398:Homeodomain Proteins
keywords: Deformed; Glia; Homeodomain; Homothorax; Hox; Mandibular; Maxillary; Neural autoregulation; Neuroblast; Neuromere; Neuron