title: Relationship between bacterial diversity and environmental parameters during composting of different raw materials.
abstract: The aim of this study was to compare the bacterial structure of seven different composts. The primary environmental factors affecting bacterial species were identified, and a strategy to enhance the abundance of uncultured bacteria through controlling relevant environmental parameters was proposed. The results showed that the physical-chemical parameters of each different pile changed in its own manner during composting, which affected the structure and succession of bacteria in different ways. DGGE profiles showed that there were 10 prominent species during composting. Among them, four species existed in all compost types, two species existed in several piles and four species were detected in a single material. Redundancy analysis results showed that bacterial species compositions were significantly influenced by C/N and moisture (p<0.05). The optimal range of C/N was 14-27. Based on these results, the primary environmental factors affecting a certain species were further identified as a potential control of bacterial diversity.
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Cui
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Shi
authors.affiliation: Laboratory of Water Environmental System Engineering, Chinese Research Academy of Environmental Science, Beijing 100012, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China. Electronic address: weizm691120@163.com.
authors.forename: Zimin
authors.initials: Z
authors.lastname: Wei
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D012987:Soil; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012987:Soil
keywords: Bacterial diversity; Composting; Denaturing gradient gel electrophoresis (DGGE); Redundancy analysis (RDA)