title: Comparison of the Chemical Composition of Subchondral Trabecular Bone of Medial Femoral Condyle between with Advanced Osteoarthritis and without Osteoarthritis.
abstract: BACKGROUND
  The purpose of this study is to investigate differences of chemical composition between subchondral bone in advanced osteoarthritic (OA) and non-OA distal femur.
  
  
  METHODS
  Twenty femurs were harvested, respectively. The subchondral trabeculae were obtained from the middle of medial articular surface of distal femurs. A 10 mm diameter cylindrical saw was used to harvest. Raman spectroscopy, a non-destructive technique, was employed to determine the chemical information of the trabecular bones in the human distal femurs.
  
  
  RESULTS
  The maximum intensity of the phosphate peak was 2,376.51±954.6 for the non-OA group and 1,936.3±831.75 for the OA group. The maximum intensity of the phosphate peak observed between the two groups was significantly different (P=0.017). The maximum intensity of the amide I peak were 474.17±253.42 for the nonOA group and 261.91±205.61 for the OA group. The maximum intensity of the amide I peak were significantly different between the two groups (P=0.042). Also, among other chemical and matrix components (Hydroxyproline,Carbonate, Amide IIIdisordered;ordered, and CH2), the spectrums showed similar significant differences in the intensity (P=0.027, P=0.014, P=0.012; P=0.038, P=0.029). Area integration were performed to determine disorder in collagen's secondary structure via amide III (alpha helix/random coil). The value of the alpha helix to random coil band area are significantly different (P=0.021) and result showing that there was a trend toward higher collagen maturity for the nonosteoarthritic bone specimens.
  
  
  CONCLUSIONS
  The result suggested that OA may affect the chemical compositions of trabecular bone, and such distinctive chemical information may be.
authors.affiliation: Department of Orthopedic Surgery, Konyang University College of Medicine, Daejeon, Korea.
authors.forename: Kwang Kyoun
authors.initials: KK
authors.lastname: Kim
authors.affiliation: Department of Orthopedic Surgery, Konyang University College of Medicine, Daejeon, Korea. ; Department of Orthopaedics, Graduate School of Medicine, Yonsei University, Seoul, Korea.
authors.forename: Yougun
authors.initials: Y
authors.lastname: Won
authors.affiliation: Department of Orthopedic Surgery, Konyang University College of Medicine, Daejeon, Korea.
authors.forename: Tae-Gyun
authors.initials: TG
authors.lastname: Kim
authors.affiliation: Medical Device Development Center, Medical Innovation Foundation, Osong, Korea.
authors.forename: Myong-Hyun
authors.initials: MH
authors.lastname: Baek
authors.affiliation: Medicine Major, Konyang University College of Medicine, Daejeon, Korea.
authors.forename: Jaewon
authors.initials: J
authors.lastname: Choi
keywords: Cartilage; Femur; Osteoarthritis; Spectrum analysis raman