title: Heatstroke Effect on Brain Heme Oxygenase-1 in Rats.
abstract: Exposure to high environmental temperature leading to increased core body temperature above 40°C and central nervous system abnormalities such as convulsions, delirium, or coma is defined as heat stroke. Studies in humans and animals indicate that the heat shock responses of the host contribute to multiple organ injury and death during heat stroke. Heme oxygenase-1 (HO-1)-a stress-responsive enzyme that catabolizes heme into iron, carbon monoxide, and biliverdin-has an important role in the neuroprotective mechanism against ischemic stroke. Here, we investigated the role of endogenous HO-1 in heat-induced brain damage in rats. RT-PCR results revealed that levels of HO-1 mRNA peaked at 0 h after heat exposure and immunoblot analysis revealed that the maximal protein expression occurred at 1 h post-heat exposure. Subsequently, we detected the HO-1 expression in the cortical brain cells and revealed the neuronal cell morphology. In conclusion, HO-1 is a potent protective molecule against heat-induced brain damage. Manipulation of HO-1 may provide a potential therapeutic approach for heat-related diseases.
authors.affiliation: 1. Department of Neurosurgery, Taipei Medical University-Wan Fang Hospital, Taipei 11696, Taiwan.
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Wen
authors.affiliation: 2. Department of Biology and Anatomy, National Defense Medical Center, Taipei 114, Taiwan.
authors.forename: Tsung-Ta
authors.initials: TT
authors.lastname: Liu
authors.affiliation: 3. Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.
authors.forename: Yuh-Feng
authors.initials: YF
authors.lastname: Lin
authors.affiliation: 4. Division of Nephrology, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan.
authors.forename: Chun-Chi
authors.initials: CC
authors.lastname: Chen
authors.affiliation: 5. Department of Neurosurgery, Lo-Hsu Foundation, Lotung Poh-Ai Hospital, Luodong, Yilan 265, Taiwan ; 6. Department of Exercise and Health Promotion, College of Education, Chinese Culture University, Taipei 11114, Taiwan.
authors.forename: Woon-Man
authors.initials: WM
authors.lastname: Kung
authors.affiliation: 7. Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan.
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Huang
authors.affiliation: 1. Department of Neurosurgery, Taipei Medical University-Wan Fang Hospital, Taipei 11696, Taiwan ; 8. Graduate Institute of Injury Prevention and Control, College of Public Health and Nutrition, Taipei Medical University, Taipei 11031, Taiwan.
authors.forename: Tien-Jen
authors.initials: TJ
authors.lastname: Lin
authors.affiliation: 3. Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan ; 9. Division of General Surgery, Department of Urology, Shuang Ho Hospital, Taipei Medical University, New Taipei City 23561, Taiwan.
authors.forename: Yuan-Hung
authors.initials: YH
authors.lastname: Wang
authors.affiliation: 1. Department of Neurosurgery, Taipei Medical University-Wan Fang Hospital, Taipei 11696, Taiwan ; 10. The PhD Program of Translational Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.
authors.forename: Li
authors.initials: L
authors.lastname: Wei
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000503:physiopathology; D002540:Cerebral Cortex / Q000201:enzymology / Q000503:physiopathology; D015971:Gene Expression Regulation, Enzymologic; D018883:Heat Stroke / Q000201:enzymology* / Q000503:physiopathology; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism*; D008297:Male; D017207:Rats, Sprague-Dawley
chemical_list: D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat
keywords: cortical brain; heat stroke; heme oxygenase-1