title: Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain.
abstract: The infectious dose of a virus pool of original US PEDV strain PC22A was determined in 4-day-old, cesarean-derived, colostrum-deprived (CDCD) piglets. The median pig diarrhea dose (PDD50) of the virus pool was determined as 7.35 log10 PDD50/mL, similar to the cell culture infectious titer, 7.75 log10 plaque-forming units (PFU)/mL. 100 PDD50 caused watery diarrhea in all conventional suckling piglets (n = 12) derived from a PEDV-naive sow, whereas 1000 and 10 000 PDD50 did not cause diarrhea in piglets derived from two PEDV-field exposed-recovered sows. This information is important for future PEDV challenge studies and validation of PEDV vaccines.
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. liu.4503@osu.edu.|State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China. liu.4503@osu.edu.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. lin.1903@osu.edu.
authors.forename: Chun-Ming
authors.initials: CM
authors.lastname: Lin
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. annamalai.5@osu.edu.
authors.forename: Thavamathi
authors.initials: T
authors.lastname: Annamalai
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. gao.898@osu.edu.
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. lu.608@buckeyemail.osu.edu.
authors.forename: Zhongyan
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. esseili.1@osu.edu.
authors.forename: Malak A
authors.initials: MA
authors.lastname: Esseili
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. jung.221@osu.edu.
authors.forename: Kwonil
authors.initials: K
authors.lastname: Jung
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. tholothvirol@mans.edu.eg.|Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt. tholothvirol@mans.edu.eg.
authors.forename: Mohamed
authors.initials: M
authors.lastname: El-Tholoth
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. saif.2@osu.edu.
authors.forename: Linda J
authors.initials: LJ
authors.lastname: Saif
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. wang.655@osu.edu.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D005260:Female; D053485:Porcine epidemic diarrhea virus / Q000472:pathogenicity* / Q000502:physiology*; D011247:Pregnancy; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014481:United States; D014774:Virulence