title: Cytotoxicity of chitosans with different acetylation degrees and molecular weights on bladder carcinoma cells.
abstract: The purpose of this research was to evaluate the cytotoxicity of chitosans with different degrees of acetylation (DA) and molecular weights (MW), as well as the effect of their positive ionic charges controlled by pH on bladder carcinoma cells (RT112 and RT112cp) using the tetrazolium salt colorimetric (MTT) assay. Our data showed that all chitosan samples were cytotoxic on RT112 and RT112cp cells with a higher cytotoxicity obtained at lower pH. Further, it was found that the toxicity increased with increasing DA. However, no significant difference in cytotoxicity between chitosans with different molecular weights was observed. Annexin V-FITC staining test was then used to study and quantify the induction of apoptosis. Data shows that chitosans induce apoptosis of RT112 and RT112cp cells with the same dependence with DA.
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, University of Sfax, National School of Engineering, P.O. Box 1173-3038, Sfax, Tunisia. Electronic address: islem.younes@gmail.com.
authors.forename: Islem
authors.initials: I
authors.lastname: Younes
authors.affiliation: AGing Imaging Modeling, CNRS FRE 3405, Grenoble Alpes University, EPHE, Grenoble, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Frachet
authors.affiliation: Biomaterials Applications, 6 rue Lesdiguières, 38000 Grenoble, France.
authors.forename: Marguerite
authors.initials: M
authors.lastname: Rinaudo
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, University of Sfax, National School of Engineering, P.O. Box 1173-3038, Sfax, Tunisia.
authors.forename: Kemel
authors.initials: K
authors.lastname: Jellouli
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, University of Sfax, National School of Engineering, P.O. Box 1173-3038, Sfax, Tunisia.
authors.forename: Moncef
authors.initials: M
authors.lastname: Nasri
mesh_terms: D000107:Acetylation; D017302:Annexins / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D002945:Cisplatin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D001749:Urinary Bladder Neoplasms
chemical_list: D017302:Annexins; D000970:Antineoplastic Agents; D048271:Chitosan; D002945:Cisplatin
keywords: Acetylation degree; Bladder cancer; Chitosan; Cytotoxicity; Molecular weight