title: MC3T3-E1 Cells Behavior on Surfaces Bombarded by Argon Ions in Planar Cathode Discharge.
abstract: To evaluate the effect of topography in nanoscale, titanium surfaces were bombarded by argon ions (a chemically inert gas), in an atmosphere of plasma. The effects of surface parameters on morphology, adhesion, proliferation, and MC3T3-E1 preosteoblasts differentiation were analyzed. Nontreated (smooth) surfaces were used as a control. The levels of average roughness (Ra) observed in bombarded and smooth titanium surfaces were of 95 and 14 nm, respectively. The wettability increased on treated surfaces. The number of attached cells (30 and 60 min) was significantly higher on the bombarded surface. The cell proliferation after 3 and 7 days was also significantly higher on the ion-bombarded surface. In addition, the ALP activity and expression of osteocalcin were higher in cells grown on the treated surface. The results showed that bombardment with argon ions increased the roughness and the wettability of the Ti surface, promoting a significant increase in the adhesion, proliferation, and differentiation of preosteoblasts.
authors.affiliation: Department of Animal Science, Federal University of Semiarid Region, Mossoró, RN, Brazil.
authors.forename: Carlos Eduardo Bezerra
authors.initials: CE
authors.lastname: Moura
authors.affiliation: Department of Animal Science, Federal University of Semiarid Region, Mossoró, RN, Brazil.
authors.forename: Naisandra Bezerra
authors.initials: NB
authors.lastname: Silva
authors.affiliation: Department of Mechanical Engineering, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Juliana Carvalho
authors.initials: JC
authors.lastname: Sa
authors.affiliation: Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Geraldo Barroso
authors.initials: GB
authors.lastname: Cavalcanti
authors.affiliation: Department of Cellular Biology and Genetics, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Silvia Regina Batistuzzo
authors.initials: SR
authors.lastname: de Medeiros
authors.affiliation: Department of Biochemistry, Federal University of Rio Grande do Norte, Natal, RN.
authors.forename: Hugo Alexandre Oliveira
authors.initials: HA
authors.lastname: Rocha
authors.affiliation: Department of Surgery, University of São Paulo, São Paulo, Brazil.
authors.forename: Paula Carvalho
authors.initials: PC
authors.lastname: Papa
authors.affiliation: Department of Mechanical Engineering, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Clodomiro
authors.initials: C
authors.lastname: Alves
mesh_terms: D000818:Animals; D001128:Argon / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D004566:Electrodes; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D058626:Plasma Gases / Q000737:chemistry; D019736:Prostheses and Implants; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D058626:Plasma Gases; D001128:Argon; D014025:Titanium
keywords: Cell differentiation; Cell morphology; Nanoscale surface; Surface modification; Surface roughness