title: Classical swine fever virus marker vaccine strain CP7_E2alf: genetic stability in vitro and in vivo.
abstract: Recently, CP7_E2alf (SuvaxynCSF Marker), a live marker vaccine against classical swine fever virus, was licensed through the European Medicines Agency. For application of such a genetically engineered virus under field conditions, knowledge about its genetic stability is essential. Here, we report on stability studies that were conducted to assess and compare the mutation rate of CP7_E2alf in vitro and in vivo. Sequence analyses upon passaging confirmed the high stability of CP7_E2alf, and no recombination events were observed in the experimental setup. The data obtained in this study confirm the genetic stability of CP7_E2alf as an important safety component.
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, 17493, Greifswald, Insel Riems, Germany.
authors.forename: Katja V
authors.initials: KV
authors.lastname: Goller
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, 17493, Greifswald, Insel Riems, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Dräger
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, 17493, Greifswald, Insel Riems, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Höper
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, 17493, Greifswald, Insel Riems, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Beer
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, 17493, Greifswald, Insel Riems, Germany. sandra.blome@fli.bund.de.
authors.forename: Sandra
authors.initials: S
authors.lastname: Blome
mesh_terms: D000818:Animals; D006691:Classical Swine Fever / Q000517:prevention & control / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000276:immunology; D042822:Genomic Instability; D013552:Swine; D022581:Vaccines, Marker / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics* / Q000276:immunology
chemical_list: D022581:Vaccines, Marker; D014765:Viral Vaccines
keywords: CP7_E2alf; Classical swine fever; Genetic stability; Marker vaccine