title: Fluorescence switchable probes based on a molecular rotor for selective detection of proteins and small molecules.
abstract: In this communication, we report a general strategy to create fluorescence switchable probes, where a small molecule ligand is conjugated to a fluorescent molecular rotor, for the selective detection of proteins through a non-enzymatic process. In the presence of target proteins, bond rotation of the molecular rotor is restricted, thereby triggering the emission of strong fluorescence.
authors.affiliation: Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang Fu Rd, Hsinchu 30013, Taiwan, Republic of China. kttan@mx.nthu.edu.tw.
authors.forename: Hsiu-Ping
authors.initials: HP
authors.lastname: Lai
authors.affiliation: Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang Fu Rd, Hsinchu 30013, Taiwan, Republic of China. kttan@mx.nthu.edu.tw.
authors.forename: Ruo-Cing
authors.initials: RC
authors.lastname: Gao
authors.affiliation: Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang Fu Rd, Hsinchu 30013, Taiwan, Republic of China. kttan@mx.nthu.edu.tw.
authors.forename: Chi-Ling
authors.initials: CL
authors.lastname: Huang
authors.affiliation: Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang Fu Rd, Hsinchu 30013, Taiwan, Republic of China. kttan@mx.nthu.edu.tw and Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, 101 Sec. 2, Kuang Fu Rd, Hsinchu 30013, Taiwan, Republic of China.
authors.forename: I-Chia
authors.initials: IC
authors.lastname: Chen
authors.affiliation: Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang Fu Rd, Hsinchu 30013, Taiwan, Republic of China. kttan@mx.nthu.edu.tw and Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, 101 Sec. 2, Kuang Fu Rd, Hsinchu 30013, Taiwan, Republic of China.
authors.forename: Kui-Thong
authors.initials: KT
authors.lastname: Tan
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D011506:Proteins / Q000032:analysis*; D011804:Quinolines / Q000737:chemistry*; D013449:Sulfonamides / Q000032:analysis*
chemical_list: D052160:Benzothiazoles; D005456:Fluorescent Dyes; D011506:Proteins; D011804:Quinolines; D013449:Sulfonamides; C051000:thiazole orange