title: LC-MS based metabolomics identification of novel biomarkers of tobacco smoke-induced chronic bronchitis.
abstract: Tobacco smoke (TS) is a major causative agent to lead to chronic bronchitis (CB). However the mechanisms of CB induced by TS are unclear. In this report, rats were exposed to different concentrations of TS and the metabolic features of CB were characterized by using a nontargeted metabolic profiling method based on liquid chromatography-mass spectrometry (LC-MS) to detect the altered metabolic patterns in serum from CB rats and investigate the mechanisms of CB. 11 potential biomarkers were identified in serum of rats. Among them, the levels of lysophosphatidylethanolamine (18:1), lysophosphatidic acid (18:1), lysophosphatidylethanolamine (18:0), lysophosphatidylethanolamine (16:0), lysophosphatidylethanolamine (20:4), docosahexaenoic acid, 5-hydroxyindoleacetic acid and 5'-carboxy-γ-tocopherol were higher in TS group compared to control group. Conversely, the levels of 4-imidazolone-5-propionic acid, 12-hydroxyeicosatetraenoic acid and uridine were lower in TS group. The results indicated that the mechanism of CB was related to amino acid metabolism and lipid metabolism, particularly lipid metabolism. In addition, lysophosphatidylethanolamines were proved to be important mediators, which could be used as biomarkers to diagnose CB. These results also suggested that metabolomics was suitable for diagnosing CB and elucidating the possible metabolic pathways of TS-induced CB.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6, WangJing ZhongHuan South Street, Chao-Yang District, Beijing, 100102, People's Republic of China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Ren
authors.affiliation: Center of Scientific Experiment, Beijing University of Chinese Medicine, No. 11 North 3rd Ring East Road, Chao-Yang District, Beijing, 100029, People's Republic of China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6, WangJing ZhongHuan South Street, Chao-Yang District, Beijing, 100102, People's Republic of China.
authors.forename: Xiaorui
authors.initials: X
authors.lastname: Fu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6, WangJing ZhongHuan South Street, Chao-Yang District, Beijing, 100102, People's Republic of China.
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Ma
authors.affiliation: Center of Scientific Experiment, Beijing University of Chinese Medicine, No. 11 North 3rd Ring East Road, Chao-Yang District, Beijing, 100029, People's Republic of China.
authors.forename: Chunguo
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6, WangJing ZhongHuan South Street, Chao-Yang District, Beijing, 100102, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6, WangJing ZhongHuan South Street, Chao-Yang District, Beijing, 100102, People's Republic of China.
authors.forename: Simin
authors.initials: S
authors.lastname: Tian
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6, WangJing ZhongHuan South Street, Chao-Yang District, Beijing, 100102, People's Republic of China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Liu
authors.affiliation: Center of Scientific Experiment, Beijing University of Chinese Medicine, No. 11 North 3rd Ring East Road, Chao-Yang District, Beijing, 100029, People's Republic of China.
authors.forename: Baosheng
authors.initials: B
authors.lastname: Zhao
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6, WangJing ZhongHuan South Street, Chao-Yang District, Beijing, 100102, People's Republic of China.
authors.forename: Xueyong
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D029481:Bronchitis, Chronic / Q000097:blood* / Q000139:chemically induced; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008246:Lysophospholipids / Q000097:blood / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012906:Smoke / Q000009:adverse effects*
chemical_list: D015415:Biomarkers; D008246:Lysophospholipids; D012906:Smoke; C008301:lysophosphatidylethanolamine; C032881:lysophosphatidic acid
keywords: LC-MS; chronic bronchitis; lysophosphatidylethanolamine; metabolomics; tobacco smoke