title: Mapping the subcellular localization of Fe3O4@TiO2nanoparticles by X-ray Fluorescence Microscopy.
abstract: The targeted delivery of Fe3O4@TiO2 nanoparticles to cancer cells is an important step in their development as nanomedicines. We have synthesized nanoparticles that can bind the Epidermal Growth Factor Receptor, a cell surface protein that is overexpressed in many epithelial type cancers. In order to study the subcellular distribution of these nanoparticles, we have utilized the sub-micron resolution of X-ray Fluorescence Microscopy to map the locationof Fe3O4@TiO2NPs and other trace metal elements within HeLa cervical cancer cells. Here we demonstrate how the higher resolution of the newly installed Bionanoprobe at the Advanced Photon Source at Argonne National Laboratory can greatly improve our ability to distinguish intracellular nanoparticles and their spatial relationship with subcellular compartments.
authors.affiliation: Department of Radiation Oncology, Northwestern University, Chicago, IL 60611, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: X-ray Sciences Division, Argonne National Laboratory, Argonne, IL 60439, USA.
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.affiliation: X-ray Sciences Division, Argonne National Laboratory, Argonne, IL 60439, USA.
authors.forename: S C
authors.initials: SC
authors.lastname: Gleber
authors.affiliation: X-ray Sciences Division, Argonne National Laboratory, Argonne, IL 60439, USA.
authors.forename: B
authors.initials: B
authors.lastname: Lai
authors.affiliation: Life Sciences Collaborative Access Team, Argonne National Laboratory, Argonne, IL 60439, USA.
authors.forename: K
authors.initials: K
authors.lastname: Brister
authors.affiliation: Xradia, Pleasanton, CA 94588, USA.
authors.forename: C
authors.initials: C
authors.lastname: Flachenecker
authors.affiliation: Department of Radiation Oncology, Northwestern University, Chicago, IL 60611, USA.
authors.forename: B
authors.initials: B
authors.lastname: Wanzer
authors.affiliation: Department of Radiation Oncology, Northwestern University, Chicago, IL 60611, USA.
authors.forename: T
authors.initials: T
authors.lastname: Paunesku
authors.affiliation: X-ray Sciences Division, Argonne National Laboratory, Argonne, IL 60439, USA.
authors.forename: S
authors.initials: S
authors.lastname: Vogt
authors.affiliation: Department of Radiation Oncology, Northwestern University, Chicago, IL 60611, USA.
authors.forename: G E
authors.initials: GE
authors.lastname: Woloschak