title: Spatial concentration distribution analysis of cells in electrode-multilayered microchannel by dielectric property measurement.
abstract: The spatial concentration distribution of cells in a microchannel is measured by combining the dielectric properties of cells with the specific structure of the electrode-multilayered microchannel. The dielectric properties of cells obtained with the impedance spectroscopy method includes the cell permittivity and dielectric relaxation, which corresponds to the cell concentration and structure. The electrode-multilayered microchannel is constructed by 5 cross-sections, and each cross-section contains 5 electrode-layers embedded with 16 micro electrodes. In the experiment, the dielectric properties of cell suspensions with different volume concentrations are measured with different electrode-combinations corresponding to different electric field distributions. The dielectric relaxations of different cell concentrations are compared and discussed with the Maxwell-Wagner dispersion theory, and the relaxation frequencies are analysed by a cell polarization model established based on the Hanai cell model. Moreover, a significant linear relationship with AC frequency dependency between relative permittivity and cell concentration was found, which provides a promising way to on-line estimate cell concentration in microchannel. Finally, cell distribution in 1 cross-section of the microchannel (X and Y directions) was measured with different electrode-combinations using the dielectric properties of cell suspensions, and cell concentration distribution along the microchannel (Z direction) was visualized at flowing state. The present cell spatial sensing study provides a new approach for 3 dimensional non-invasive online cell sensing for biological industry.
authors.affiliation: Department of Mechanical Engineering, Chiba University , Chiba 263-0022, Japan.
authors.forename: Jiafeng
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Mechanical Engineering, Chiba University , Chiba 263-0022, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kodera
authors.affiliation: Department of Mechanical Engineering, Tokyo Metropolitan University , Tokyo 192-0397, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Obara
authors.affiliation: Department of Mechanical Engineering, Chiba University , Chiba 263-0022, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Sugawara
authors.affiliation: Department of Mechanical Engineering, Chiba University , Chiba 263-0022, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takei