title: Core-shell fibrous stem cell carriers incorporating osteogenic nanoparticulate cues for bone tissue engineering.
abstract: Moldable hydrogels that incorporate stem cells hold great promise for tissue engineering. They secure the encapsulated cells for required periods while allowing a permeable exchange of nutrients and gas with the surroundings. Core-shell fibrous structured hydrogel system represents these properties relevant to stem cell delivery and defect-adjustable tissue engineering. A designed dual concentric nozzle is used to simultaneously deposit collagen and alginate with a core-shell structured continuous fiber form in the ionic calcium bath. We aimed to impart extrinsic osteogenic cues in the nanoparticulate form, i.e., bioactive glass nanoparticles (BGn), inside the alginate shell, while encapsulating rat mesenchymal stem cells in the collagen core. Ionic measurement in aqueous solution indicated a continuous release of calcium ions from the BGn-added and -free scaffolds, whereas silicon was only released from the BGn-containing scaffolds. The presence of BGn allowed higher number of cells to migrate into the scaffolds when implanted in subcutaneous tissues of rat. Cell viability was preserved in the presence of the BGn, with no significant differences noticed from the control. The presence of BGn enhanced the osteogenic differentiation of the encapsulated rat mesenchymal stem cells, presenting higher levels of alkaline phosphatase activity as well as bone related genes, including collagen type I, bone sialoprotein and osteocalcin. Taken together, the incorporated BGn potentiated the capacity of the core-shell fibrous hydrogel system to deliver stem cells targeting bone tissue engineering.
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, South Korea.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Olmos Buitrago
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, South Korea. Electronic address: romanp@dankook.ac.kr.
authors.forename: Roman A
authors.initials: RA
authors.lastname: Perez
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, South Korea.
authors.forename: Ahmed
authors.initials: A
authors.lastname: El-Fiqi
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, South Korea.
authors.forename: Rajendra K
authors.initials: RK
authors.lastname: Singh
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea.
authors.forename: Joong-Hyun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, South Korea; Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan 330-714, South Korea. Electronic address: kimhw@dku.edu.
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001846:Bone Development; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials
keywords: Cell delivery; Core–shell design; Hydrogels; Osteogenic differentiation; Tissue engineering