title: Two ATP Binding Cassette G Transporters, Rice ATP Binding Cassette G26 and ATP Binding Cassette G15, Collaboratively Regulate Rice Male Reproduction.
abstract: Male reproduction in higher plants requires the support of various metabolites, including lipid molecules produced in the innermost anther wall layer (the tapetum), but how the molecules are allocated among different anther tissues remains largely unknown. Previously, rice (Oryza sativa) ATP binding cassette G15 (ABCG15) and its Arabidopsis (Arabidopsis thaliana) ortholog were shown to be required for pollen exine formation. Here, we report the significant role of OsABCG26 in regulating the development of anther cuticle and pollen exine together with OsABCG15 in rice. Cytological and chemical analyses indicate that osabcg26 shows reduced transport of lipidic molecules from tapetal cells for anther cuticle development. Supportively, the localization of OsABCG26 is on the plasma membrane of the anther wall layers. By contrast, OsABCG15 is polarly localized in tapetal plasma membrane facing anther locules. osabcg26 osabcg15 double mutant displays an almost complete absence of anther cuticle and pollen exine, similar to that of osabcg15 single mutant. Taken together, we propose that OsABCG26 and OsABCG15 collaboratively regulate rice male reproduction: OsABCG26 is mainly responsible for the transport of lipidic molecules from tapetal cells to anther wall layers, whereas OsABCG15 mainly is responsible for the export of lipidic molecules from the tapetal cells to anther locules for pollen exine development.
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (G.Z., J.S., W.L., F.X., Q.L., L.Z., G.Q., M.C., D.Z.);Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (L.S.); andSchool of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Guochao
authors.identifier: http://orcid.org/0000-0002-2825-9554
authors.initials: G
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (G.Z., J.S., W.L., F.X., Q.L., L.Z., G.Q., M.C., D.Z.);Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (L.S.); andSchool of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Jianxin
authors.identifier: http://orcid.org/0000-0002-7717-0863
authors.initials: J
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (G.Z., J.S., W.L., F.X., Q.L., L.Z., G.Q., M.C., D.Z.);Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (L.S.); andSchool of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Wanqi
authors.identifier: http://orcid.org/0000-0002-9938-5793
authors.initials: W
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (G.Z., J.S., W.L., F.X., Q.L., L.Z., G.Q., M.C., D.Z.);Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (L.S.); andSchool of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Feiyang
authors.identifier: http://orcid.org/0000-0002-8427-1195
authors.initials: F
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (G.Z., J.S., W.L., F.X., Q.L., L.Z., G.Q., M.C., D.Z.);Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (L.S.); andSchool of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Qian
authors.identifier: http://orcid.org/0000-0002-4173-5762
authors.initials: Q
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (G.Z., J.S., W.L., F.X., Q.L., L.Z., G.Q., M.C., D.Z.);Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (L.S.); andSchool of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Lu
authors.initials: L
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (G.Z., J.S., W.L., F.X., Q.L., L.Z., G.Q., M.C., D.Z.);Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (L.S.); andSchool of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Guorun
authors.identifier: http://orcid.org/0000-0001-5977-4172
authors.initials: G
authors.lastname: Qu
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (G.Z., J.S., W.L., F.X., Q.L., L.Z., G.Q., M.C., D.Z.);Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (L.S.); andSchool of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Mingjiao
authors.initials: M
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (G.Z., J.S., W.L., F.X., Q.L., L.Z., G.Q., M.C., D.Z.);Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (L.S.); andSchool of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Lukas
authors.identifier: http://orcid.org/0000-0001-7003-9929
authors.initials: L
authors.lastname: Schreiber
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (G.Z., J.S., W.L., F.X., Q.L., L.Z., G.Q., M.C., D.Z.);Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (L.S.); andSchool of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.) zhangdb@sjtu.edu.cn.
authors.forename: Dabing
authors.identifier: http://orcid.org/0000-0002-1764-2929
authors.initials: D
authors.lastname: Zhang
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000378:metabolism; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000502:physiology / Q000648:ultrastructure; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000502:physiology / Q000648:ultrastructure; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011058:Pollen / Q000235:genetics / Q000254:growth & development / Q000502:physiology / Q000648:ultrastructure; D012098:Reproduction
chemical_list: D018528:ATP-Binding Cassette Transporters; D010940:Plant Proteins