title: Production of a Bilayered Self-Assembled Skin Substitute Using a Tissue-Engineered Acellular Dermal Matrix.
abstract: Our bilayered self-assembled skin substitutes (SASS) are skin substitutes showing a structure and functionality very similar to native human skin. These constructs are used, in life-threatening burn wounds, as permanent autologous grafts for the treatment of such affected patients even though their production is exacting. We thus intended to shorten their current production time to improve their clinical applicability. A self-assembled decellularized dermal matrix (DM) was used. It allowed the production of an autologous skin substitute from patient's cells. The characterization of SASS reconstructed using a decellularized dermal matrix (SASS-DM) was performed by histology, immunofluorescence, transmission electron microscopy, and uniaxial tensile analysis. Using the SASS-DM, it was possible to reduce the standard production time from about 8 to 4 and a half weeks. The structure, cell differentiation, and mechanical properties of the new skin substitutes were shown to be similar to the SASS. The decellularization process had no influence on the final microstructure and mechanical properties of the DM. This model, by enabling the production of a skin substitute in a shorter time frame without compromising its intrinsic tissue properties, represents a promising addition to the currently available burn and wound treatments.
authors.affiliation: 1 Centre de recherche du CHU de Québec-Université Laval, axe médecine régénératrice and Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX , Quebec, Quebec, Canada .|2 Department of Surgery, Faculty of Medicine, Université Laval , Quebec, Quebec, Canada .|3 Plastic Surgery Department, Université de Montréal , Montreal, Quebec, Canada .
authors.forename: Chanel
authors.initials: C
authors.lastname: Beaudoin Cloutier
authors.affiliation: 1 Centre de recherche du CHU de Québec-Université Laval, axe médecine régénératrice and Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX , Quebec, Quebec, Canada .|2 Department of Surgery, Faculty of Medicine, Université Laval , Quebec, Quebec, Canada .
authors.forename: Rina
authors.initials: R
authors.lastname: Guignard
authors.affiliation: 1 Centre de recherche du CHU de Québec-Université Laval, axe médecine régénératrice and Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX , Quebec, Quebec, Canada .|2 Department of Surgery, Faculty of Medicine, Université Laval , Quebec, Quebec, Canada .
authors.forename: Geneviève
authors.initials: G
authors.lastname: Bernard
authors.affiliation: 1 Centre de recherche du CHU de Québec-Université Laval, axe médecine régénératrice and Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX , Quebec, Quebec, Canada .|2 Department of Surgery, Faculty of Medicine, Université Laval , Quebec, Quebec, Canada .|4 Centre Québécois sur les Matériaux Fonctionnels (CQMF) , Laval, Québec, Canada .
authors.forename: Robert
authors.initials: R
authors.lastname: Gauvin
authors.affiliation: 1 Centre de recherche du CHU de Québec-Université Laval, axe médecine régénératrice and Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX , Quebec, Quebec, Canada .|2 Department of Surgery, Faculty of Medicine, Université Laval , Quebec, Quebec, Canada .
authors.forename: Danielle
authors.initials: D
authors.lastname: Larouche
authors.affiliation: 1 Centre de recherche du CHU de Québec-Université Laval, axe médecine régénératrice and Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX , Quebec, Quebec, Canada .|2 Department of Surgery, Faculty of Medicine, Université Laval , Quebec, Quebec, Canada .
authors.forename: Amélie
authors.initials: A
authors.lastname: Lavoie
authors.affiliation: 1 Centre de recherche du CHU de Québec-Université Laval, axe médecine régénératrice and Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX , Quebec, Quebec, Canada .|2 Department of Surgery, Faculty of Medicine, Université Laval , Quebec, Quebec, Canada .
authors.forename: Dan
authors.initials: D
authors.lastname: Lacroix
authors.affiliation: 1 Centre de recherche du CHU de Québec-Université Laval, axe médecine régénératrice and Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX , Quebec, Quebec, Canada .|2 Department of Surgery, Faculty of Medicine, Université Laval , Quebec, Quebec, Canada .
authors.forename: Véronique J
authors.initials: VJ
authors.lastname: Moulin
authors.affiliation: 1 Centre de recherche du CHU de Québec-Université Laval, axe médecine régénératrice and Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX , Quebec, Quebec, Canada .|2 Department of Surgery, Faculty of Medicine, Université Laval , Quebec, Quebec, Canada .
authors.forename: Lucie
authors.initials: L
authors.lastname: Germain
authors.affiliation: 1 Centre de recherche du CHU de Québec-Université Laval, axe médecine régénératrice and Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX , Quebec, Quebec, Canada .|2 Department of Surgery, Faculty of Medicine, Université Laval , Quebec, Quebec, Canada .
authors.forename: François A
authors.initials: FA
authors.lastname: Auger
mesh_terms: D062088:Acellular Dermis; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D008297:Male; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*