title: LGN Directs Interphase Endothelial Cell Behavior via the Microtubule Network.
abstract: Angiogenic sprouts require coordination of endothelial cell (EC) behaviors as they extend and branch. Microtubules influence behaviors such as cell migration and cell-cell interactions via regulated growth and shrinkage. Here we investigated the role of the mitotic polarity protein LGN in EC behaviors and sprouting angiogenesis. Surprisingly, reduced levels of LGN did not affect oriented division of EC within a sprout, but knockdown perturbed overall sprouting. At the cell level, LGN knockdown compromised cell-cell adhesion and migration. EC with reduced LGN levels also showed enhanced growth and stabilization of microtubules that correlated with perturbed migration. These results fit a model whereby LGN influences interphase microtubule dynamics in endothelial cells to regulate migration, cell adhesion, and sprout extension, and reveal a novel non-mitotic role for LGN in sprouting angiogenesis.
authors.affiliation: Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina, United States of America.
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Wright
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, North Carolina, United States of America.
authors.forename: Erich J
authors.initials: EJ
authors.lastname: Kushner
authors.affiliation: Department of Neurology, Institute of Molecular Medicine and Genetics, Georgia Regents University, Augusta, Georgia, United States of America.
authors.forename: Quansheng
authors.initials: Q
authors.lastname: Du
authors.affiliation: Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina, United States of America; Department of Biology, University of North Carolina, Chapel Hill, North Carolina, United States of America; McAllister Heart Institute, University of North Carolina, Chapel Hill, North Carolina, United States of America; Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, United States of America.
authors.forename: Victoria L
authors.initials: VL
authors.lastname: Bautch
mesh_terms: D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007399:Interphase; D047908:Intracellular Signaling Peptides and Proteins / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism*; D018919:Neovascularization, Physiologic
chemical_list: C102754:GPSM2 protein, human; D047908:Intracellular Signaling Peptides and Proteins