title: 3D Conducting Polymer Platforms for Electrical Control of Protein Conformation and Cellular Functions.
abstract: We report the fabrication of three dimensional (3D) macroporous scaffolds made from poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) via an ice-templating method. The scaffolds offer tunable pore size and morphology, and are electrochemically active. When a potential is applied to the scaffolds, reversible changes take place in their electrical doping state, which in turn enables precise control over the conformation of adsorbed proteins (e.g.,fibronectin). Additionally, the scaffolds support the growth of mouse fibroblasts (3T3-L1) for 7 days, and are able to electrically control cell adhesion and pro-angiogenic capability. These 3D matrix-mimicking platforms offer precise control of protein conformation and major cell functions, over large volumes and long cell culture times. As such, they represent a new tool for biological research with many potential applications in bioelectronics, tissue engineering, and regenerative medicine.
authors.affiliation: Department of Materials Science and Engineering, Cornell University, Ithaca, NY 14853 USA ; Department of Biomedical Engineering, Cornell University, Ithaca, NY 14853 USA.
authors.forename: Alwin Ming-Doug
authors.initials: AM
authors.lastname: Wan
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, Gardanne 13541 France.
authors.forename: Sahika
authors.initials: S
authors.lastname: Inal
authors.affiliation: Department of Materials Science and Engineering, Cornell University, Ithaca, NY 14853 USA.
authors.forename: Tiffany
authors.initials: T
authors.lastname: Williams
authors.affiliation: Department of Materials Science and Engineering, Cornell University, Ithaca, NY 14853 USA ; Department of Biomedical Engineering, Cornell University, Ithaca, NY 14853 USA.
authors.forename: Karin
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, Gardanne 13541 France ; MicroVitae Technologies, Pôle d'Activité Y. Morandat, 1480 rue d'Arménie, Gardanne 13120 France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Leleux
authors.affiliation: Department of Materials Science and Engineering, Cornell University, Ithaca, NY 14853 USA.
authors.forename: Luis
authors.initials: L
authors.lastname: Estevez
authors.affiliation: Department of Materials Science and Engineering, Cornell University, Ithaca, NY 14853 USA.
authors.forename: Emmanuel P
authors.initials: EP
authors.lastname: Giannelis
authors.affiliation: Department of Biomedical Engineering, Cornell University, Ithaca, NY 14853 USA.
authors.forename: Claudia
authors.initials: C
authors.lastname: Fischbach
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, Gardanne 13541 France.
authors.forename: George G
authors.initials: GG
authors.lastname: Malliaras
authors.affiliation: Department of Materials Science and Engineering, Cornell University, Ithaca, NY 14853 USA ; Department of Biomedical Engineering, Cornell University, Ithaca, NY 14853 USA.
authors.forename: Delphine
authors.initials: D
authors.lastname: Gourdon