title: Development and evaluation of a Salmonella typhimurium flagellin based chimeric DNA vaccine against infectious bursal disease of poultry.
abstract: Infectious bursal disease (IBD) is an acute immunosuppressive disease of young chicks, caused by a double-stranded RNA virus. VP2 being the major capsid protein of the virus is an ideal vaccine candidate possessing the neutralizing epitopes. The present study involves the use of flagellin (fliC) as a genetic adjuvant to improve the immune response of VP2 based DNA vaccine against IBD. Our findings revealed that birds immunized with plasmid pCIVP2fliC showed robust immune response than pCIVP2 immunized groups. Further, challenge study proved that genetic fusion of fliC and VP2 can provide a comparatively higher level of protection against vvIBDV challenge in chickens than VP2 alone. These results thus indicate that Salmonella flagellin could enhance the immune responses and protection efficacy of a DNA vaccine candidate against IBDV infection in chickens, highlighting the potential of flagellin as a genetic adjuvant in the prevention of vvIBDV infection.
authors.affiliation: Recombinant DNA Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India. Electronic address: drrajibdeb@gmail.com.
authors.forename: Rajib
authors.initials: R
authors.lastname: Deb
authors.affiliation: Recombinant DNA Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Sohini
authors.initials: S
authors.lastname: Dey
authors.affiliation: Recombinant DNA Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: C
authors.initials: C
authors.lastname: Madhan Mohan
authors.affiliation: Recombinant DNA Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Satish
authors.initials: S
authors.lastname: Gaikwad
authors.affiliation: Recombinant DNA Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Nitin
authors.initials: N
authors.lastname: Kamble
authors.affiliation: Recombinant DNA Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Sagar A
authors.initials: SA
authors.lastname: Khulape
authors.affiliation: Recombinant DNA Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Shishir Kumar
authors.initials: SK
authors.lastname: Gupta
authors.affiliation: Recombinant DNA Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Hemanta Kumar
authors.initials: HK
authors.lastname: Maity
authors.affiliation: Recombinant DNA Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Dinesh Chandra
authors.initials: DC
authors.lastname: Pathak
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D018175:Birnaviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D036022:Capsid Proteins / Q000276:immunology; D002645:Chickens; D005408:Flagellin / Q000276:immunology; D007243:Infectious bursal disease virus / Q000276:immunology*; D010957:Plasmids; D011201:Poultry Diseases / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000378:metabolism*; D019444:Vaccines, DNA / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D036022:Capsid Proteins; D019444:Vaccines, DNA; D014765:Viral Vaccines; D005408:Flagellin
keywords: DNA vaccine; IBDV; Poultry; Salmonella; VP2; fliC