title: Arrested replication forks guide retrotransposon integration.
abstract: Long terminal repeat (LTR) retrotransposons are an abundant class of genomic parasites that replicate by insertion of new copies into the host genome. Fungal LTR retrotransposons prevent mutagenic insertions through diverse targeting mechanisms that avoid coding sequences, but conserved principles guiding their target site selection have not been established. Here, we show that insertion of the fission yeast LTR retrotransposon Tf1 is guided by the DNA binding protein Sap1 and that the efficiency and location of the targeting depend on the activity of Sap1 as a replication fork barrier. We propose that Sap1 and the fork arrest it causes guide insertion of Tf1 by tethering the integration complex to target sites.
authors.affiliation: Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Nelson A133, 604 Allison Road, Piscataway, NJ 08854, USA.
authors.forename: Jake Z
authors.initials: JZ
authors.lastname: Jacobs
authors.affiliation: Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Nelson A133, 604 Allison Road, Piscataway, NJ 08854, USA.
authors.forename: Jesus D
authors.initials: JD
authors.lastname: Rosado-Lugo
authors.affiliation: Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Nelson A133, 604 Allison Road, Piscataway, NJ 08854, USA.
authors.forename: Susanne
authors.initials: S
authors.lastname: Cranz-Mileva
authors.affiliation: Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Nelson A133, 604 Allison Road, Piscataway, NJ 08854, USA.
authors.forename: Keith M
authors.initials: KM
authors.lastname: Ciccaglione
authors.affiliation: Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Nelson A133, 604 Allison Road, Piscataway, NJ 08854, USA.
authors.forename: Vincent
authors.initials: V
authors.lastname: Tournier
authors.affiliation: Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Nelson A133, 604 Allison Road, Piscataway, NJ 08854, USA. zaratiegui@dls.rutgers.edu.
authors.forename: Mikel
authors.initials: M
authors.lastname: Zaratiegui
mesh_terms: D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D018626:Retroelements / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D020079:Terminal Repeat Sequences / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D018626:Retroelements; C085981:Sap1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins