title: The cyclic AMP receptor protein (CRP) regulates mqsRA, coding for the bacterial toxin-antitoxin gene pair, in Escherichia coli.
abstract: Bacterial persisters represent a small number of slow-growing antibiotic-tolerant cells among populations of rapidly growing cells, and are the main cause of frequent recurrent infections. MqsR-MqsA, the toxin-antitoxin (TA) pair, is the most frequently induced TA system associated with antibiotic persistence in Escherichia coli. In this study, we show that the cyclic AMP receptor protein (CRP) indirectly upregulates mqsRA transcription. We also show that CRP plays an important role in antibiotic persistence, which seems to be partially mediated through MqsRA. Overall, this study highlights the role of CRP as an important regulator of antibiotic persistence in E. coli.
authors.affiliation: Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. Electronic address: sheetal@barc.gov.in.
authors.forename: Sheetal
authors.initials: S
authors.lastname: Uppal
authors.affiliation: Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.
authors.forename: Narendra
authors.initials: N
authors.lastname: Jawali
mesh_terms: D002373:Cyclic AMP Receptor Protein / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D002373:Cyclic AMP Receptor Protein; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C555039:MqsA protein, E coli; C508571:MqsR protein, E coli
keywords: Antibiotic; CRP; Coli; Persister; Toxin; mqsR