title: Gelsolin inhibits the proliferation and invasion of the 786-0 clear cell renal cell carcinoma cell line in vitro.
abstract: The aim of the present study was to investigate the effect of gelsolin (GSN) on the proliferation and invasion of the 786-0 clear cell renal cell carcinoma (ccRCC) cell line in vitro. A GSN overexpression lentiviral vector was constructed and transfected into 786‑0 ccRCC cells in vitro. A 3-(4,5-dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay was conducted to detect the effect of GSN on the proliferation and adhesion ability of the 786‑0 ccRCC cells, and a Transwell invasion assay was used to determine the effect of GSN on the invasion of 786‑0 ccRCC cells. In addition, the expression levels of invasion‑associated proteins, matrix metalloproteinase (MMP)2, MMP9 and E‑cadherin were analyzed by ELISA and western blotting. The MTT assay demonstrated a significantly lower optical density value for the 786‑0/GSN cells compared with that of the 786‑0/green fluorescent protein (GFP) and 786‑0 cells following 24‑ and 48‑h culture (P<0.05). The mean penetration rate of the 786‑0/GSN cells was significantly lower than that of the 786‑0/GFP and 786‑0 cells (P<0.05) according to the Transwell invasion assay. The expression levels of MMP2 and MMP9 were significantly decreased in the 786‑0/GSN cells, when compared with the 786‑0/GFP and 786‑0 cells following a 48‑h transfection, according to ELISA (P<0.001). Furthermore, in the 786‑0/GSN cells, the expression levels of MMP2 and MMP9 were markedly decreased, while the expression of E‑cadherin was markedly increased. Thus, the overexpression of GSN may inhibit the proliferation, adhesion ability and invasion of 786‑0 ccRCC cells. Additionally, GSN downregulated the expression of MMP2 and MMP9, and upregulated the expression of E‑cadherin in the 786‑0 ccRCC cells, which may have suppressed the invasion ability of the 786-0 ccRCC cells.
authors.affiliation: Department of Dermatology, The First Hospital of Harbin, Harbin, Heilongjiang 150010, P.R. China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150089, P.R. China.
authors.forename: Limin
authors.initials: L
authors.lastname: Cai
authors.affiliation: Department of Orthopaedic Surgery, The First Hospital of Harbin, Harbin, Heilongjiang 150010, P.R. China.
authors.forename: Qinggang
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Pathology, Basic Medical Science College, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Jin
mesh_terms: D015820:Cadherins / Q000032:analysis; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018260:Gelsolin / Q000235:genetics*; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D006801:Humans; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D014162:Transfection; D015854:Up-Regulation
chemical_list: D015820:Cadherins; D018260:Gelsolin; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9