title: Establishment and characterization of a rat model of hyperphosphatemia.
abstract: We established a rat model of hyperphosphatemia and investigated the systemic effects of high phosphorus (P). Sprague Dawley rats were randomly divided into high (HP), low (LP), and normal (NP) P groups (N = 12 each), which received injections of fructose diphosphate sodium, or were fed self-manufactured low phosphorus or normal diets, respectively. In each group, 4 rats were sacrificed at the first, third, and sixth week to detect the serum (Scr) and urinary creatinine and P, and calcium (Ca) levels. The HP group's serum P and intact parathyroid hormone (iPTH) were significantly higher than those in the other groups at the first, third, and sixth weeks, (P < 0.05); the LP group's serum P was lower than the NP group's at the third week (P < 0.05), while at the sixth week, the serum P and iPTH were lower (P < 0.05). No significant differences were detected for blood Ca+ (P > 0.05). The HP group's Scr increased (P < 0.01), whereas the fractional excretion decreased (P < 0.05) significantly. Thighbone and lumbar spine bone densities differed significantly between groups in the third week (P < 0.05); LP group densities were lower than NP group measures (P < 0.05). Crystallized stones were not observed microscopically following hematoxylin and eosin staining of the kidney. We successfully established a hyperphosphatemia rat model, and high blood P was found to significantly influence renal function and bone density. These results might provide a foundation to study the effects of hyperphosphatemia in rats.
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Q-G
authors.initials: QG
authors.lastname: Zhu
authors.affiliation: Quality Control Office, The Fifth Hospital of Cheng Du, Cheng Du, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: C-L
authors.initials: CL
authors.lastname: Jin
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: H-P
authors.initials: HP
authors.lastname: Wang
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: C
authors.initials: C
authors.lastname: Yu
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Y K
authors.initials: YK
authors.lastname: Zhao
authors.affiliation: Urology Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: G J
authors.initials: GJ
authors.lastname: Yuan
authors.affiliation: Urology Department, The Fifth Hospital of Cheng Du, Cheng Du, China.
authors.forename: X-P
authors.initials: XP
authors.lastname: Hu
authors.affiliation: Urology Department, The Fifth Hospital of Cheng Du, Cheng Du, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Urology Department, The Fifth Hospital of Cheng Du, Cheng Du, China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D015519:Bone Density; D002130:Calcium Phosphates / Q000097:blood; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D005269:Femur / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D054559:Hyperphosphatemia / Q000097:blood* / Q000000981:diagnostic imaging; D007668:Kidney / Q000473:pathology; D010281:Parathyroid Hormone / Q000097:blood; D011859:Radiography; D017207:Rats, Sprague-Dawley
chemical_list: D002130:Calcium Phosphates; D010281:Parathyroid Hormone; C020243:calcium phosphate; D003404:Creatinine