title: Lung volume recruitment in a preterm pig model of lung immaturity.
abstract: A translational preterm pig model analogous to infants born at 28 wk of gestation revealed that continuous positive airway pressure results in limited lung recruitment but does not prevent respiratory distress syndrome, whereas assist-control + volume guarantee (AC+VG) ventilation improves recruitment but can cause injury, highlighting the need for improved ventilation strategies. We determined whether airway pressure release ventilation (APRV) can be used to recruit the immature lungs of preterm pigs without injury. Spontaneously breathing pigs delivered at 89% of term (model for 28-wk infants) were randomized to 24 h of APRV (n = 9) vs. AC+VG with a tidal volume of 5 ml/kg (n = 10). Control pigs (n = 36) were provided with supplemental oxygen by an open mask. Nutrition and fluid support was provided throughout the 24-h period. All pigs supported with APRV and AC+VG survived 24 h, compared with 62% of control pigs. APRV resulted in improved lung volume recruitment compared with AC+VG based on radiographs, lower Pco2 levels (44 ± 2.9 vs. 53 ± 2.7 mmHg, P = 0.009) and lower inspired oxygen fraction requirements (36 ± 6 vs. 44 ± 11%, P < 0.001), and higher oxygenation index (5.1 ± 1.5 vs. 2.9 ± 1.1, P = 0.001). There were no differences between APRV and AC+VG pigs for heart rate, ratio of wet to dry lung mass, proinflammatory cytokines, or histopathological markers of lung injury. Lung protective ventilation with APRV improved recruitment of alveoli of preterm lungs, enhanced development and maintenance of functional residual capacity without injury, and improved clinical outcomes relative to AC+VG. Long-term consequences of lung volume recruitment by using APRV should be evaluated.
authors.affiliation: Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee;
authors.forename: Esmond L
authors.initials: EL
authors.lastname: Arrindell
authors.affiliation: Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee;
authors.forename: Ramesh
authors.initials: R
authors.lastname: Krishnan
authors.affiliation: School of Health Studies, University of Memphis, Memphis, Tennessee;
authors.forename: Marie
authors.initials: M
authors.lastname: van der Merwe
authors.affiliation: Dräger Medical Inc., Telford, Pennsylvania; and.
authors.forename: Frank
authors.initials: F
authors.lastname: Caminita
authors.affiliation: School of Health Studies, University of Memphis, Memphis, Tennessee;
authors.forename: Scott C
authors.initials: SC
authors.lastname: Howard
authors.affiliation: Pathology, University of Tennessee Health Science Center, Memphis, Tennessee.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Health Studies, University of Memphis, Memphis, Tennessee; rbddngtn@memphis.edu.
authors.forename: Randal K
authors.initials: RK
authors.lastname: Buddington
mesh_terms: D000818:Animals; D045422:Continuous Positive Airway Pressure; D005260:Female; D008168:Lung / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology; D011247:Pregnancy; D047928:Premature Birth; D011859:Radiography; D012127:Respiratory Distress Syndrome, Newborn / Q000000981:diagnostic imaging / Q000517:prevention & control*; D034421:Sus scrofa; D013990:Tidal Volume
keywords: animal model; development; prematurity; pulmonary