title: Parameters influencing the photo-induced electron transfer from tryptophan-containing peptides to a Ru(II) complex: a systematic study.
abstract: Ruthenium(II) polyazaaromatic complexes have gained interest in recent decades as biomolecular tools, especially in the development of new phototherapeutic agents. These light emissive Ru complexes based on π-deficient ligands were first designed to allow a photo-induced electron transfer (PET) with the guanine base in DNA since their (3)MLCT state is highly photo-oxidizing. Later the field of research was extended to proteins with the highlighting of a PET process with the tryptophan residue. This paper reports the kinetics of the luminescence quenching of [Ru(TAP)2phen](2+) by several selected peptide sequences containing at least one tryptophan residue. By using a peptide library we highlight the important parameters influencing the kinetics of the photo-electron transfer process, such as the net electrostatic charge and the number of tryptophan residues. The best peptide candidates were selected to study the formation of photo-products by MALDI-ToF mass spectrometry. A high photoreactivity of the [Ru(TAP)2phen](2+) complex was observed and multiple photoadducts were characterized, among them inter-peptidic adducts as well as intra-peptidic adducts.
authors.affiliation: Laboratoire de Chimie Organique et Photochimie CP160/08, Université libre de Bruxelles, 50 Av. Franklin D. Roosevelt, 1050 Brussels, Belgium. cmouche@ulb.ac.be.
authors.forename: Kevin
authors.initials: K
authors.lastname: Garnir
authors.affiliation: Laboratoire de Chimie Organique et Photochimie CP160/08, Université libre de Bruxelles, 50 Av. Franklin D. Roosevelt, 1050 Brussels, Belgium. cmouche@ulb.ac.be.
authors.forename: Sandra
authors.initials: S
authors.lastname: Estalayo-Adrián
authors.affiliation: Université Grenoble Alpes, Département de Chimie Moléculaire UMR CNRS 5250, 38000 Grenoble, France. eric.defrancq@ujf-grenoble.fr.
authors.forename: Rémy
authors.initials: R
authors.lastname: Lartia
authors.affiliation: Service de Synthèse et de Spectrométire de Masse Organiques, Université de Mons, 23 Place du Parc, 7000 Mons, Belgium. pascal.gerbaux@umons.ac.be.
authors.forename: Julien
authors.initials: J
authors.lastname: De Winter
authors.affiliation: Université Grenoble Alpes, Département de Chimie Moléculaire UMR CNRS 5250, 38000 Grenoble, France. eric.defrancq@ujf-grenoble.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Defrancq
authors.affiliation: Laboratory for Chemistry of Novel Materials, Centre of Innovation and Research in Materials and Polymers, Université de Mons-UMONS, 20 Place du Parc, 7000 Mons, Belgium. mathieu.surin@umons.ac.be.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Surin
authors.affiliation: Laboratory for Chemistry of Novel Materials, Centre of Innovation and Research in Materials and Polymers, Université de Mons-UMONS, 20 Place du Parc, 7000 Mons, Belgium. mathieu.surin@umons.ac.be.
authors.forename: Vincent
authors.initials: V
authors.lastname: Lemaur
authors.affiliation: Service de Synthèse et de Spectrométire de Masse Organiques, Université de Mons, 23 Place du Parc, 7000 Mons, Belgium. pascal.gerbaux@umons.ac.be.
authors.forename: Pascal
authors.initials: P
authors.lastname: Gerbaux
authors.affiliation: Laboratoire de Chimie Organique et Photochimie CP160/08, Université libre de Bruxelles, 50 Av. Franklin D. Roosevelt, 1050 Brussels, Belgium. cmouche@ulb.ac.be.
authors.forename: Cécile
authors.initials: C
authors.lastname: Moucheron
mesh_terms: D056831:Coordination Complexes / Q000737:chemistry*; D004583:Electrons; D015394:Molecular Structure; D055668:Photochemical Processes; D012428:Ruthenium / Q000737:chemistry*; D014364:Tryptophan / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D012428:Ruthenium; D014364:Tryptophan