title: Intestinal stem cell growth and differentiation on a tubular scaffold with evaluation in small and large animals.
abstract: OBJECTIVE
  To investigate the growth and differentiation of intestinal stem cells on a novel tubular scaffold in vitro and in vivo.
  
  
  METHODS
  Intestinal progenitor cells from mice or humans were cultured with myofibroblasts, macrophages and/or bacteria, and evaluated in mice via omental implantation. Mucosal regeneration was evaluated in dogs after rectal mucosectomy followed by scaffold implantation.
  
  
  RESULTS
  Intestinal progenitor cells differentiated into crypt-villi structures on the scaffold. Differentiation and scaffold coverage was enhanced by coculture with myofibroblasts, macrophages and probiotic bacteria, while the implanted scaffolds enhanced mucosal regeneration in the dog rectum.
  
  
  CONCLUSIONS
  Intestinal stem cell growth and differentiation on a novel tubular scaffold is enhanced through addition of cellular and microbial components, as validated in mice and dogs.
authors.affiliation: Division of Pediatric Surgery, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA 15217, USA.
authors.forename: Shahab A
authors.initials: SA
authors.lastname: Shaffiey
authors.affiliation: Division of Pediatric Surgery, Department of Surgery, Johns Hopkins University, 600 N Wolfe Street, Baltimore, MD 21287, USA.
authors.forename: Hongpeng
authors.initials: H
authors.lastname: Jia
authors.affiliation: McGowan Institute for Regenerative Medicine, 450 Technology Drive Suite 300, Pittsburgh, PA 15219, USA.
authors.forename: Timothy
authors.initials: T
authors.lastname: Keane
authors.affiliation: Department of Biological & Environmental Engineering, Cornell University, Ithaca, NY 14850, USA.
authors.forename: Cait
authors.initials: C
authors.lastname: Costello
authors.affiliation: Division of Pediatric Surgery, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA 15217, USA.
authors.forename: Deena
authors.initials: D
authors.lastname: Wasserman
authors.affiliation: Division of Pediatric Surgery, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA 15217, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Quidgley
authors.affiliation: McGowan Institute for Regenerative Medicine, 450 Technology Drive Suite 300, Pittsburgh, PA 15219, USA.
authors.forename: Jenna
authors.initials: J
authors.lastname: Dziki
authors.affiliation: McGowan Institute for Regenerative Medicine, 450 Technology Drive Suite 300, Pittsburgh, PA 15219, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Badylak
authors.affiliation: Division of Pediatric Surgery, Department of Surgery, Johns Hopkins University, 600 N Wolfe Street, Baltimore, MD 21287, USA.
authors.forename: Chhinder P
authors.initials: CP
authors.lastname: Sodhi
authors.affiliation: Department of Biological & Environmental Engineering, Cornell University, Ithaca, NY 14850, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: March
authors.affiliation: Division of Pediatric Surgery, Department of Surgery, Johns Hopkins University, 600 N Wolfe Street, Baltimore, MD 21287, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Hackam
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000473:pathology; D002454:Cell Differentiation; D049109:Cell Proliferation; D018920:Coculture Techniques; D004195:Disease Models, Animal; D004285:Dogs; D004926:Escherichia coli / Q000502:physiology; D007249:Inflammation / Q000473:pathology; D007421:Intestine, Small / Q000166:cytology*; D019344:Lactic Acid / Q000737:chemistry; D007778:Lactobacillus / Q000502:physiology; D008810:Mice, Inbred C57BL; D008871:Microvilli / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D055153:Stem Cell Niche; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: artificial intestine; intestinal stem cells; necrotizing enterocolitis; scaffold; short bowel syndrome; tissue-engineered small intestine