title: Automatic microfluidic fluorescence-array measurement system for detecting organic phosphate.
abstract: In this study, an automatic microfluidic fluorescence-array measurement system is developed to detect the concentration of organic phosphate based on the luminol-hydrogen peroxide catalytic fluorescent mechanism. Not only sample quantity and cost can be reduced, but also detection time, accuracy and precision can be improved in the system. The system is composed of a CCD image module, a stepper motor with driver, a microfluidic fluorescence array, a background light elimination module, and a dynamic image-analyzed interface. The pesticides of chlorpyrifos and fenitrothion of organic phosphate are chosen as experimental samples. Only a 2.5 μ l quantity of sample is required to have a fast response time of 1.4 second. Experimental results show that the sensitivities of chlorpyrifos and fenitrothion are 1.88 V/ppm in the range of 0.166 ∼ 10 ppm with averaged error of 1.66% and 0.32 V/ppm in the range of 0.03 ∼ 10 ppm with averaged error of 1.68% respectively. The organophosphorus effective detection range of the developed system covers the legal prescription for pesticide residues.
authors.affiliation: Department of Automatic Control Engineering, Feng-Chia University, Taichung, Taiwan.
authors.forename: Hsing-Cheng
authors.initials: HC
authors.lastname: Chang
authors.affiliation: Ph.D. Program of Electrical and Communications Engineering, Feng-Chia University, Taichung, Taiwan.
authors.forename: Jung-Chin
authors.initials: JC
authors.lastname: Lin
authors.affiliation: Department of Automatic Control Engineering, Feng-Chia University, Taichung, Taiwan.
authors.forename: Shyan-Lung
authors.initials: SL
authors.lastname: Lin
authors.affiliation: Department of Automatic Control Engineering, Feng-Chia University, Taichung, Taiwan.
authors.forename: I-Nan
authors.initials: IN
authors.lastname: Chang
authors.affiliation: Department of Automatic Control Engineering, Feng-Chia University, Taichung, Taiwan.
authors.forename: Chern-Sheng
authors.initials: CS
authors.lastname: Lin
authors.affiliation: Department of Automatic Control Engineering, Feng-Chia University, Taichung, Taiwan.
authors.forename: Shi-Yao
authors.initials: SY
authors.lastname: Chen
mesh_terms: D004390:Chlorpyrifos / Q000032:analysis*; D005278:Fenitrothion / Q000032:analysis*; D005453:Fluorescence; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D010755:Organophosphates / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*
chemical_list: D010755:Organophosphates; D010573:Pesticide Residues; D004390:Chlorpyrifos; D005278:Fenitrothion
keywords: Organophosphorus measurement; centrifugal microfluidic; chlorpyrifos and fenitrothion; fluorescence-array