title: Isolation of Guttiferones from Renewable Parts of Symphonia globulifera by Centrifugal Partition Chromatography.
abstract: The aim of this study was to investigate the species Symphonia globulifera, a source of polycyclic polyprenylated acyl phloroglucinols such as guttiferone A, which is known to exhibit a variety of biological activities including noticeable antileishmanial properties. Our goal was the identification and the quantification of guttiferone A in different renewable parts of S. globulifera and its preparative isolation. To the best of our knowledge, there is no data concerning its mechanism of action. Consequently, it is particularly interesting to isolate it in gram quantities in order to establish structure activity relationship studies. After performing high-performance liquid chromatography profiles detecting the presence of guttiferone A and proceeding to its quantification, a centrifugal partition chromatography methodology using a two-phase solvent system of cyclohexane/ethyl acetate/methanol/water (20 :  1 :  20 : 1, v/v/v/v) was applied to each extract. In conclusion, a centrifugal partition chromatography system has been developed to ensure a fast, reliable, and scalable way to isolate, with a high level of purity, guttiferone A from five renewable parts of S. globulifera. Moreover, this methodology can be extended to the isolation of other polycyclic polyprenylated acyl phloroglucinols such as guttiferones B, C, and D.
authors.affiliation: Laboratoire de Pharmacognosie, UMR CNRS 8638 COMETE (Chimie Organique, Médicinale, Extractive, Toxicologie et Environnement), Université Paris Descartes Sorbonne Paris Cité, Paris, France.
authors.forename: Kevin
authors.initials: K
authors.lastname: Cottet
authors.affiliation: Laboratoire de Pharmacognosie, UMR CNRS 8638 COMETE (Chimie Organique, Médicinale, Extractive, Toxicologie et Environnement), Université Paris Descartes Sorbonne Paris Cité, Paris, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Fromentin
authors.affiliation: Laboratoire de Pharmacognosie, UMR CNRS 8638 COMETE (Chimie Organique, Médicinale, Extractive, Toxicologie et Environnement), Université Paris Descartes Sorbonne Paris Cité, Paris, France.
authors.forename: Marina
authors.initials: M
authors.lastname: Kritsanida
authors.affiliation: Laboratoire de Pharmacognosie, UMR CNRS 8638 COMETE (Chimie Organique, Médicinale, Extractive, Toxicologie et Environnement), Université Paris Descartes Sorbonne Paris Cité, Paris, France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Grougnet
authors.affiliation: CNRS Guyane, USR 3456, Cayenne, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Odonne
authors.affiliation: Laboratoire des Substances Naturelles Amazoniennes, Institut Pasteur de la Guyane, UMR EcoFoG (Ecologie des forêts de Guyane), Cayenne, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Duplais
authors.affiliation: Laboratoire de Pharmacognosie, UMR CNRS 8638 COMETE (Chimie Organique, Médicinale, Extractive, Toxicologie et Environnement), Université Paris Descartes Sorbonne Paris Cité, Paris, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Michel
authors.affiliation: Laboratoire de Pharmacognosie, UMR CNRS 8638 COMETE (Chimie Organique, Médicinale, Extractive, Toxicologie et Environnement), Université Paris Descartes Sorbonne Paris Cité, Paris, France.
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Lallemand
mesh_terms: D001577:Benzophenones / Q000737:chemistry / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D028084:Clusiaceae / Q000737:chemistry*; D015394:Molecular Structure
chemical_list: D001577:Benzophenones