title: Isolation and Fast Selective Determination of Nor-abietanoid Diterpenoids from Perovskia atriplicifolia Roots Using LC-ESI-MS/MS with Multiple Reaction Monitoring.
abstract: In the first part of this study we extracted, isolated, and identified the main diterpenoid constituents from the roots of a Central Asian medicinal and ornamental plant--Perovskia atriplicifolia Benth. Eight major nor-abietanoid pigments were obtained using NP silica gel column chromatography and preparative RP-HPLC: cryptotanshinone, 1-hydroxycryptotanhinone, miltirone, 1-oxomiltirone, tanshinone IIa, 1,2 didehydrotanshinone IIa, 1,2 didehydromiltirone, the non-quinone diterpenoid - arucadiol, as well as rosmarinic acid as a main phenolic compound. Secondly, we used the obtained compounds for fast and selective determination of the main diterpenes present in P. atriplicifolia root extract. After extraction with n-hexane, the quantitative analysis was carried out by LC-MS/MS with a triple quadrupole (qQq) mass detector without any prior clean-up step. Identification of the diterpenes was confirmed by multiple reaction monitoring (MRM) using the most representative transitions from the precursor ions, while the most sensitive transitions were used for quantification in a 19-minute run. Most of the isolated and analyzed compounds had not been previously reported from this species. This easily cultivated plant is a promising source of several pharmacologically valuable abietanoid diterpenoids.
authors.forename: Sylwester
authors.initials: S
authors.lastname: Slusarczyk
authors.forename: Jakub
authors.initials: J
authors.lastname: Topolski
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Domaradzki
authors.forename: Michael
authors.initials: M
authors.lastname: Adams
authors.forename: Matthias
authors.initials: M
authors.lastname: Hamburger
authors.forename: Adam
authors.initials: A
authors.lastname: Matkowski
mesh_terms: D045784:Abietanes / Q000302:isolation & purification; D002853:Chromatography, Liquid; D002934:Cinnamates / Q000302:isolation & purification; D053630:Depsides / Q000302:isolation & purification; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification*; D019686:Lamiaceae / Q000737:chemistry*; D010616:Phenanthrenes / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D000097123:Rosmarinic Acid
chemical_list: D045784:Abietanes; D002934:Cinnamates; D053630:Depsides; D004224:Diterpenes; D010616:Phenanthrenes; D000097123:Rosmarinic Acid; C021751:tanshinone; C068880:miltirone; C037886:cryptotanshinone