title: MCPIP1 Regulates Fibroblast Migration in 3-D Collagen Matrices Downstream of MAP Kinases and NF-κB.
abstract: The fibroblast-populated three-dimensional (3-D) collagen matrix has been used to model matrix contraction, cell motility, and general fibroblast biology. MCPIP1 (monocyte chemotactic protein-induced protein 1) has been shown to regulate inflammation, angiogenesis, and cellular motility. In the present study, we demonstrated induction of MCPIP1 in human fibroblasts embedded in the stress-released 3-D collagen matrix, which occurred through activation of mitogen-activated protein kinases, phosphoinositide 3-kinase, and NF-κB. Furthermore, MCPIP1 induction was associated with inhibition of fibroblast migration out of the nested collagen matrix. MCPIP1 induction or ectopic expression also upregulated p53. RNA interference of p53 prevented the inhibition of migration produced by induction or ectopic expression of MCPIP1. Our findings suggest a new role for MCPIP1 as a molecular switch that regulates fibroblast migration in the nested collagen matrix model.
authors.affiliation: Department of Surgery, University of Nebraska Medical Center, Omaha, Nebraska, USA; Department of Physiology, School of Medicine, Southeast University, Nanjing, Jiangsu, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chao
authors.affiliation: Department of Physiology, School of Medicine, Southeast University, Nanjing, Jiangsu, China.
authors.forename: Xiaoniu
authors.initials: X
authors.lastname: Dai
authors.affiliation: Department of Surgery, University of Nebraska Medical Center, Omaha, Nebraska, USA.
authors.forename: Tiffany
authors.initials: T
authors.lastname: Peña
authors.affiliation: Department of Surgery, University of Nebraska Medical Center, Omaha, Nebraska, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Doyle
authors.affiliation: Department of Surgery, University of Nebraska Medical Center, Omaha, Nebraska, USA.
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Guenther
authors.affiliation: Department of Surgery, University of Nebraska Medical Center, Omaha, Nebraska, USA; Department of Surgery, VA Nebraska-Western Iowa Health Care System, Omaha, Nebraska, USA; Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, Nebraska, USA. Electronic address: macarlso@unmc.edu.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Carlson
mesh_terms: D002465:Cell Movement; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000502:physiology*; D016328:NF-kappa B / Q000502:physiology*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D012260:Ribonucleases / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D016159:Tumor Suppressor Protein p53 / Q000502:physiology
chemical_list: D016328:NF-kappa B; C495901:TP53 protein, human; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D003094:Collagen; D051057:Proto-Oncogene Proteins c-akt; D020928:Mitogen-Activated Protein Kinases; D012260:Ribonucleases; C510646:ZC3H12A protein, human