title: Synthesis of deuterium-labelled substrates for the study of oleuropein biosynthesis in Olea europaea callus cultures.
abstract: We propose the cell culture approach to investigate oleuropein (1) biogenesis in Olea europaea L. We suggest employing olive callus cultures to identify the iridoidic precursor of oleuropein. In fact, we confirmed that callus cells from olive shoot explants are able to produce key secoiridoid as 1. To enable this approach, we synthesised and characterised deuterium-labelled iridoidic precursors belonging both to the loganin and the 8-epiloganin series. These iridoids are [7,8-(2)H2]-7-deoxy-8-epi-loganin (2(D)), [8,10-(2)H2]-8-epi-loganin (4(D)) and [7,8-(2)H2]-7-deoxy-loganin (3(D)).
authors.affiliation: a Dipartimento di Chimica , Università di Roma "La Sapienza" , Roma , Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Serrilli
authors.affiliation: a Dipartimento di Chimica , Università di Roma "La Sapienza" , Roma , Italy.
authors.forename: Agnese
authors.initials: A
authors.lastname: Maggi
authors.affiliation: a Dipartimento di Chimica , Università di Roma "La Sapienza" , Roma , Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Casagrande
authors.affiliation: a Dipartimento di Chimica , Università di Roma "La Sapienza" , Roma , Italy.
authors.forename: Armandodoriano
authors.initials: A
authors.lastname: Bianco
mesh_terms: D003903:Deuterium / Q000737:chemistry*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000737:chemistry*; D015394:Molecular Structure; D031658:Olea / Q000737:chemistry*; D046509:Tissue Culture Techniques
chemical_list: D057888:Iridoid Glucosides; D039823:Iridoids; C002769:oleuropein; D003903:Deuterium; C059516:loganin
keywords: ESI-MS; Iridoid; biogenesis; deuterium label; oleuropein