title: Phosphoproteomics Analysis of Endometrium in Women with or without Endometriosis.
abstract: BACKGROUND
  The molecular mechanisms underlying the endometriosis are still not completely understood. In order to test the hypothesis that the approaches in phosphoproteomics might contribute to the identification of key biomarkers to assess disease pathogenesis and drug targets, we carried out a phosphoproteomics analysis of human endometrium.
  
  
  METHODS
  A large-scale differential phosphoproteome analysis, using peptide enrichment of titanium dioxide purify and sequential elution from immobilized metal affinity chromatography with linear trap quadrupole-tandem mass spectrometry, was performed in endometrium tissues from 8 women with or without endometriosis.
  
  
  RESULTS
  The phosphorylation profiling of endometrium from endometriosis patients had been obtained, and found that identified 516 proteins were modified at phosphorylation level during endometriosis. Gene ontology annotation analysis showed that these proteins were enriched in cellular processes of binding and catalytic activity. Further pathway analysis showed that ribosome pathway and focal adhesion pathway were the top two pathways, which might be deregulated during the development of endometriosis.
  
  
  CONCLUSIONS
  That large-scale phosphoproteome quantification has been successfully identified in endometrium tissues of women with or without endometriosis will provide new insights to understand the molecular mechanisms of the development of endometriosis.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Xu
authors.forename: Hai-Teng
authors.initials: HT
authors.lastname: Deng
authors.forename: Chong-Dong
authors.initials: CD
authors.lastname: Liu
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D002846:Chromatography, Affinity; D004715:Endometriosis / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D010750:Phosphoproteins / Q000032:analysis; D010766:Phosphorylation; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D010750:Phosphoproteins