title: Bacterial Diversity Analysis during the Fermentation Processing of Traditional Chinese Yellow Rice Wine Revealed by 16S rDNA 454 Pyrosequencing.
abstract: Rice wine is a traditional Chinese fermented alcohol drink. Spontaneous fermentation with the use of the Chinese starter and wheat Qu lead to the growth of various microorganisms during the complete brewing process. It's of great importance to fully understand the composition of bacteria diversity in rice wine in order to improve the quality and solve safety problems. In this study, a more comprehensive bacterial description was shown with the use of bacteria diversity analysis, which enabled us to have a better understanding. Rarefaction, rank abundance, alpha Diversity, beta diversity and principal coordinates analysis simplified their complex bacteria components and provide us theoretical foundation for further investigation. It has been found bacteria diversity is more abundant at mid-term and later stage of brewing process. Bacteria community analysis reveals there is a potential safety hazard existing in the fermentation, since most of the sequence reads are assigned to Enterobacter (7900 at most) and Pantoea (7336 at most), followed by Staphylococcus (2796 at most) and Pseudomonas (1681 at most). Lactic acid bacteria are rare throughout the fermentation process which is not in accordance with other reports. This work may offer us an opportunity to investigate micro ecological fermentation system in food industry.
authors.affiliation: Dept. of Food Science and Nutrition, Zhejiang Univ, Hangzhou, 310058, China.
authors.forename: Ruo-si
authors.initials: RS
authors.lastname: Fang
authors.affiliation: Dept. of Food Science and Nutrition, Zhejiang Univ, Hangzhou, 310058, China.
authors.forename: Ya-chen
authors.initials: YC
authors.lastname: Dong
authors.affiliation: Dept. of Food Science and Nutrition, Zhejiang Univ, Hangzhou, 310058, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Food Science and Human Nutrition, Clemson Univ, S.C, 29634, U.S.A.
authors.forename: Qi-he
authors.initials: QH
authors.lastname: Chen
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000254:growth & development*; D044822:Biodiversity; D019149:Bioreactors; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal; D002523:Edible Grain / Q000382:microbiology; D004754:Enterobacter / Q000235:genetics / Q000254:growth & development; D005285:Fermentation; D006801:Humans; D007777:Lactobacillaceae / Q000235:genetics / Q000254:growth & development; D012275:Oryza / Q000382:microbiology*; D020636:Pantoea / Q000235:genetics / Q000254:growth & development; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D014920:Wine / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Chinese yellow rice wine 454 pyrosequencing; bacterial diversity analysis