title: Isolation of Dickeya dadantii strains from potato disease and biocontrol by their bacteriophages.
abstract: One of the most economically important bacterial pathogens of plants and plant products is Dickeya dadantii. This bacterium causes soft rot disease in tubers and other parts of the potato and other plants of the Solanaceae family. The application of restricted host range bacteriophages as biocontrol agents has recently gained widespread interest. This study purposed to isolate the infectious agent of the potato and evaluate its biocontrol by bacteriophages. Two phytopathogenic strains were isolated from infected potatoes, identified based on biochemical and 16S rRNA gene sequencing, and submitted to GenBank as D. dadantii strain pis3 (accession no. HQ423668) and D. dadantii strain sip4 (accession no. HQ423669). Their bacteriophages were isolated from Caspian Sea water by enriching the water filtrate with D. dadantii strains as hosts using spot or overlay methods. On the basis of morphotypes, the isolated bacteriophages were identified as members of the Myoviridae and Siphoviridae families and could inhibit the growth of antibiotic resistant D. dadantii strains in culture medium. Moreover, in Dickeya infected plants treated with bacteriophage, no disease progression was detected. No significant difference was seen between phage-treated and control plants. Thus, isolated bacteriophages can be suggested for the biocontrol of plant disease caused by Dickeya strains.
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Islamic Republic of Iran, Iran .
authors.forename: Abbas
authors.initials: A
authors.lastname: Soleimani-Delfan
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Islamic Republic of Iran, Iran .
authors.forename: Zahra
authors.initials: Z
authors.lastname: Etemadifar
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Islamic Republic of Iran, Iran .
authors.forename: Giti
authors.initials: G
authors.lastname: Emtiazi
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Islamic Republic of Iran, Iran .
authors.forename: Majid
authors.initials: M
authors.lastname: Bouzari
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000302:isolation & purification*; D001483:Base Sequence; D061046:Biological Control Agents / Q000145:classification / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D016974:Dickeya chrysanthemi / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification / Q000821:virology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017900:Myoviridae / Q000145:classification / Q000302:isolation & purification; D010935:Plant Diseases / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017906:Siphoviridae / Q000145:classification / Q000302:isolation & purification; D011198:Solanum tuberosum / Q000382:microbiology*
chemical_list: D061046:Biological Control Agents; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Dickeya dadantii; antibiotic resistance; bacteriophage; biocontrol; soft rot