title: The ARF2-ANT-COR15A gene cascade regulates ABA-signaling-mediated resistance of large seeds to drought in Arabidopsis.
abstract: Seedlings of large-seeded plants are considered to be able to withstand abiotic stresses efficiently. The molecular mechanisms that underlie the involved signaling crosstalk between the large-seeded trait and abiotic tolerance are, however, largely unknown. Here, we demonstrate the molecular link that integrates plant abscisic acid (ABA) responses to drought stress into the regulation of seed mass. Both loss-of-function mutants of the Auxin Response Factor 2 (ARF2 encoding a transcription factor) and lines overexpressing AINTEGUMENTA (ANT; a transcription factor) under the 35S promoter exhibited large seed and drought-tolerant phenotypes as a result of abnormal ABA-auxin crosstalk signaling pathways in Arabidopsis. The target gene COLD-REGULATED15A (COR15a) was identified as participating in the regulation of seed development with ABA signaling through a negative regulation mechanism that is mediated by ANT. The molecular and genetic evidence presented indicate that ARF2, ANT and COR15A form an ABA-mediated signaling pathway to link modulation of seed mass with drought tolerance. These observations indicate that the ARF2 transcription factor serves as a molecular link that integrates plant ABA responses to drought stress into the regulation of seed mass.
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming 650201, People's Republic of China menglaisheng@mail.kib.ac.cn liuaizhong@mail.kib.ac.cn.
authors.forename: Lai-Sheng
authors.initials: LS
authors.lastname: Meng
authors.affiliation: School of Bioengineering and Biotechnology, Tianshui Normal University, TianShui City 741001, People's Republic of China.
authors.forename: Zhi-Bo
authors.initials: ZB
authors.lastname: Wang
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming 650201, People's Republic of China.
authors.forename: Shun-Qiao
authors.initials: SQ
authors.lastname: Yao
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming 650201, People's Republic of China menglaisheng@mail.kib.ac.cn liuaizhong@mail.kib.ac.cn.
authors.forename: Aizhong
authors.initials: A
authors.lastname: Liu
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012639:Seeds / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C099055:AINTEGUMENTA protein, Arabidopsis; C503421:ARF2 protein, Arabidopsis; D029681:Arabidopsis Proteins; C116166:COR15 protein, Arabidopsis; D012097:Repressor Proteins; D014157:Transcription Factors; D000040:Abscisic Acid
keywords: ABA signal; AINTEGUMENTA; ANT; ARF2; Auxin response factor 2; COLD-REGULATED15A; COR15A; Drought tolerance; Seed mass