title: Polyozellin, a key constituent of the edible mushroom Polyozellus multiplex, attenuates glutamate-induced mouse hippocampal neuronal HT22 cell death.
abstract: Polyozellus multiplex (PM), a Korean edible mushroom, has biological activities such as chemoprevention of stomach cancer, inhibition of lipid peroxidation, and reduction of prolyl endopeptidase activity. However, there are little reports on the protective effects of PM or its constituents against glutamate-induced mouse hippocampal neuronal cell (HT22) death. In this study, polyozellin (PZ), a key constituent of PM, was applied to glutamate-treated HT22 cells to evaluate its neuroprotective mechanisms. PZ (25 μM) dramatically increased the HT22 cell viability when the cell death was induced by 5 mM glutamate for 12 h, which was mediated by inhibition of Ca(2+) influx, intracellular reactive oxygen species (ROS) production, and lipid peroxidation. PZ also regulated expression of Bid, Bcl-2, and apoptosis-inducing factor (AIF), as well as phosphorylation of mitogen-activated protein kinases (MAPKs). These data suggest that PM and its constituent PZ might be useful for prevention and treatment of neurodegenerative disorders.
authors.affiliation: Research Institute of Pharmaceutical Sciences, College of Pharmacy, Kyungpook National University, 80 Daehak-ro, Sankyuk-dong, Daegu 702-701, Republic of Korea. kssong@knu.ac.kr.
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Yang
authors.forename: Kyung-Sik
authors.initials: KS
authors.lastname: Song
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051033:Apoptosis Inducing Factor / Q000235:genetics / Q000378:metabolism; D051023:BH3 Interacting Domain Death Agonist Protein / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005663:Furans / Q000494:pharmacology*; D018698:Glutamic Acid / Q000633:toxicity*; D006624:Hippocampus / Q000166:cytology*; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D010766:Phosphorylation; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D051033:Apoptosis Inducing Factor; D051023:BH3 Interacting Domain Death Agonist Protein; C494815:Bid protein, mouse; D005663:Furans; D018696:Neuroprotective Agents; C494830:AIFM1 protein, mouse; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; C109015:polyozellin; C052617:Bcl2 protein, mouse; D018698:Glutamic Acid; D020928:Mitogen-Activated Protein Kinases