title: High prevalence of the MYD88 mutation in testicular lymphoma: Immunohistochemical and genetic analyses.
abstract: The activating mutation of MYD88 has been identified in diffuse large B-cell lymphoma (DLBCL). We investigated the mutational status and both the gene amplification and protein expression of MYD88 in 23 cases of testicular DLBCL. To detect the MYD88 mutations, we employed the allele-specific PCR and Sanger sequencing. MYD88 gene amplification and protein expression were analyzed by quantitative PCR and by immunohistochemistry, respectively. There were 17 cases of primary testicular DLBCL: 94% (16/17) exhibited a non-Germinal center B-cell (non-GCB) subtype, 82% (14/17) showed the MYD88 L265P, and 65% (11/17) had intense expression of MYD88. When compared with normal lymph nodes, the MYD88 is significantly amplified in primary testicular DLBCL. However, the amplification status showed no correlation with its mutational status or protein expression. Moreover, neither the MYD88 mutational status nor the expression pattern affected overall survival. Six cases were secondary testicular DLBCL with an 83% (5/6) and an 80% (4/5) incidence of the non-GCB subtype and of the MYD88 L265P, respectively. In conclusion, we demonstrated a high prevalence of the non-GCB subtype and the common MYD88 L265P in both primary and secondary testicular DLBCL. Our data suggest that the MYD88 mutation is a fairly consistent genetic feature in testicular DLBCL.
authors.affiliation: Department of Pathology, University of Yamanashi, Chuo, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Oishi
authors.affiliation: Department of Pathology, University of Yamanashi, Chuo, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kondo
authors.affiliation: Department of Pathology, University of Yamanashi, Chuo, Japan.
authors.forename: Tadao
authors.initials: T
authors.lastname: Nakazawa
authors.affiliation: Department of Pathology, University of Yamanashi, Chuo, Japan.
authors.forename: Kunio
authors.initials: K
authors.lastname: Mochizuki
authors.affiliation: Division of Pathology, Iwata City Hospital, Iwata, Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Tanioka
authors.affiliation: Department of Diagnostic Pathology, Yamanashi Prefectural Central Hospital, Kofu, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Oyama
authors.affiliation: Department of Pathology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Department of Urology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Junpei
authors.initials: J
authors.lastname: Iizuka
authors.affiliation: Department of Urology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Tanabe
authors.affiliation: Department of Pathology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Shibata
authors.affiliation: Department of Hematology and Oncology, University of Yamanashi, Chuo, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Kirito
authors.affiliation: Department of Pathology, University of Yamanashi, Chuo, Japan.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Katoh
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000473:pathology; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D016403:Lymphoma, Large B-Cell, Diffuse / Q000453:epidemiology* / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics*; D015995:Prevalence; D013736:Testicular Neoplasms / Q000453:epidemiology* / Q000235:genetics* / Q000473:pathology
chemical_list: C507779:MYD88 protein, human; D053594:Myeloid Differentiation Factor 88
keywords: MYD88; diffuse large B-cell lymphoma; gene amplification; immunohistochemistry; mutation; testis