title: Topography-Guided Proliferation: Distinct Surface Microtopography Increases Proliferation of Chondrocytes In Vitro.
abstract: Chondrocyte-based cartilage repair techniques require control of articular chondrocyte expansion ex vivo. Articular chondrocytes have limited availability, and prolonged culturing to obtain a cell number sufficient for clinical use often results in phenotypic alterations and increased costs. In this study, we applied a screening library consisting of micrometer-sized topographical features, termed biosurface structure array (BSSA), to identify specific topographical microstructures affecting the proliferation of human chondrocytes in passage 1 (P1) or 2 (P2). The BSSA library comprised 10 patterns and 16 combinations of pillar size (X) and interpillar gap size (Y). Specific microstructures significantly increased the chondrocytes' proliferative responsiveness in term of patterns, X and Y for P2 compared with P1. The P1 and P2 chondrocytes responded independently to similar patterns after 4 days of culturing, whereas only chondrocytes at P2 responded to specific microstructures with Y = 1 μm and X = 2, 4 μm by a 2.3- and 4.4-fold increased proliferation, respectively. In conclusion, these findings indicate that specific surface topographies promote chondrocyte proliferation and may, indeed, be a tool to control the behavior of chondrocytes in vitro.
authors.affiliation: 1 Orthopaedic Research Laboratory, Aarhus University Hospital , Aarhus, Denmark .
authors.forename: Natasja Leth
authors.initials: NL
authors.lastname: Joergensen
authors.affiliation: 1 Orthopaedic Research Laboratory, Aarhus University Hospital , Aarhus, Denmark .
authors.forename: Dang Quang Svend
authors.initials: DQ
authors.lastname: Le
authors.affiliation: 2 Interdisciplinary Nanoscience Centre, iNANO, Aarhus University , Aarhus, Denmark .
authors.forename: Ole Zoffmann
authors.initials: OZ
authors.lastname: Andersen
authors.affiliation: 2 Interdisciplinary Nanoscience Centre, iNANO, Aarhus University , Aarhus, Denmark .
authors.forename: Morten
authors.initials: M
authors.lastname: Foss
authors.affiliation: 3 Department of Anatomy, Institute of Biomedicine, Aarhus University , Aarhus, Denmark .
authors.forename: Carl Christian
authors.initials: CC
authors.lastname: Danielsen
authors.affiliation: 1 Orthopaedic Research Laboratory, Aarhus University Hospital , Aarhus, Denmark .
authors.forename: Casper Bindzus
authors.initials: CB
authors.lastname: Foldager
authors.affiliation: 4 Sports Trauma Clinic, Aarhus University Hospital , Aarhus, Denmark .
authors.forename: Martin
authors.initials: M
authors.lastname: Lind
authors.affiliation: 1 Orthopaedic Research Laboratory, Aarhus University Hospital , Aarhus, Denmark .
authors.forename: Helle
authors.initials: H
authors.lastname: Lysdahl
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D049109:Cell Proliferation / Q000502:physiology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D048091:Guided Tissue Regeneration / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008422:Materials Testing; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials