title: Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine.
abstract: Quantitative cysteine-independent ligation of a Gd(3+) tag to genetically encoded p-azido-L-phenylalanine via Cu(I)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd(3+)-Gd(3+) distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd(3+) ion relative to the protein can be predicted with high accuracy.
authors.affiliation: Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia. gottfried.otting@anu.edu.au.
authors.forename: E H
authors.initials: EH
authors.lastname: Abdelkader
authors.forename: A
authors.initials: A
authors.lastname: Feintuch
authors.forename: X
authors.initials: X
authors.lastname: Yao
authors.forename: L A
authors.initials: LA
authors.lastname: Adams
authors.forename: L
authors.initials: L
authors.lastname: Aurelio
authors.forename: B
authors.initials: B
authors.lastname: Graham
authors.forename: D
authors.initials: D
authors.lastname: Goldfarb
authors.forename: G
authors.initials: G
authors.lastname: Otting
mesh_terms: D000595:Amino Acid Sequence; D001386:Azides / Q000737:chemistry; D002352:Carrier Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D057930:Click Chemistry; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000737:chemistry*; D005682:Gadolinium; D005971:Glutamates / Q000737:chemistry*; D016297:Mutagenesis, Site-Directed; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics; D017434:Protein Structure, Tertiary; D013113:Spin Labels
chemical_list: D001386:Azides; D002352:Carrier Proteins; D005971:Glutamates; D013113:Spin Labels; C027162:4-azidophenylalanine; D010649:Phenylalanine; D005682:Gadolinium