title: [Designation and evaluation of antisense oligodeoxynucleotides targeted to glial glutamate transporter-1a].
abstract: OBJECTIVE
  The present study was undertaken to design antisense oligodeoxynucleotides (AS-ODNs) of glial glutamate transporter-la (GLT-1a) and to evaluate the effectiveness of the designed AS-ODNs on the expression of GLT-1a.
  
  
  METHODS
  Five sequences of GLT-1a AS-ODNs were designed according to the C terminus specific sequences of GLT-1a mRNA using antisense design software of IDT Com- pany. Western blot analysis was used to evaluate the inhibition effects of the five GLT-1a AS-ODNs on the expression of GLT-la.
  
  
  RESULTS
  The sequence of GLT-1a AS-ODNs with sequence of 5'-GGTTCTTCCTCAACACTGCA-3' could specifically inhibit the expression of GLT-1a in the hippocampal CA1 subfield of rats, while it had no effect on the expression of GLT-1b. This sequence showed similar inhibition on the expression of GLT-la in sham and ceftriaxone (Cef)-treated rats. It could also significantly inhibit the cerebral ischemic preconditioning (CIP)-induced up-regulation in the expression of GLT-1a. The magnitude of the inhibition in sham, Cef- or CIP-treated rats was similar by more than 60%.
  
  
  CONCLUSIONS
  From the designed five sequences of GLT-1a AS-ODNs, we obtained an effective sequence which can specifically inhibit the expression of GLT-1a.
authors.forename: Li-zhe
authors.initials: LZ
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Yi-xian
authors.initials: YX
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Cui
authors.forename: Yu-yan
authors.initials: YY
authors.lastname: Hu
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Li
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000378:metabolism*; D027342:Excitatory Amino Acid Transporter 2 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D019194:Ischemic Preconditioning; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D012333:RNA, Messenger; D051381:Rats; D015854:Up-Regulation
chemical_list: D027342:Excitatory Amino Acid Transporter 2; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger