title: Quantitative analysis of platelets aggregates in 3D by digital holographic microscopy.
abstract: Platelet spreading and retraction play a pivotal role in the platelet plugging and the thrombus formation. In routine laboratory, platelet function tests include exhaustive information about the role of the different receptors present at the platelet surface without information on the 3D structure of platelet aggregates. In this work, we develop, a method in Digital Holographic Microscopy (DHM) to characterize the platelet and aggregate 3D shapes using the quantitative phase contrast imaging. This novel method is suited to the study of platelets physiology in clinical practice as well as the development of new drugs.
authors.affiliation: Laboratoire de Médecine Expérimentale (ULB 222 Unit), Medicine Faculty, Université libre de Bruxelles, CHU de Charleroi, Belgium.
authors.forename: Karim Zouaoui
authors.initials: KZ
authors.lastname: Boudejltia
authors.affiliation: Laboratoire de Médecine Expérimentale (ULB 222 Unit), Medicine Faculty, Université libre de Bruxelles, CHU de Charleroi, Belgium.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ribeiro de Sousa
authors.affiliation: Laboratoire de Médecine Expérimentale (ULB 222 Unit), Medicine Faculty, Université libre de Bruxelles, CHU de Charleroi, Belgium.
authors.forename: Pierrick
authors.initials: P
authors.lastname: Uzureau
authors.affiliation: Microgravity Research Centre, Université libre de Bruxelles, Brussels, Belgium.
authors.forename: Catherine
authors.initials: C
authors.lastname: Yourassowsky
authors.affiliation: Laboratory of Molecular Parasitology, IBMM, Université Libre de Bruxelles (ULB), Gosselies, Belgium.
authors.forename: David
authors.initials: D
authors.lastname: Perez-Morga
authors.affiliation: CREATIS, CNRS UMR 5220, INSERM U1044, UCB Lyon1, INSA Lyon, University of Lyon, Lyon, France.
authors.forename: Guy
authors.initials: G
authors.lastname: Courbebaisse
authors.affiliation: Informatic Department, University of Geneva, Geneva, Switzerland.
authors.forename: Bastien
authors.initials: B
authors.lastname: Chopard
authors.affiliation: Microgravity Research Centre, Université libre de Bruxelles, Brussels, Belgium.
authors.forename: Frank
authors.initials: F
authors.lastname: Dubois
keywords: (090.1995) Digital holography; (170.1530) Cell analysis; (180.0180) Microscopy