title: Sphingomonas hengshuiensis sp. nov., isolated from lake wetland.
abstract: A polyphasic taxonomic study was undertaken to establish the status of a novel bacterium, designated strain WHSC-8T, which was isolated from soil of Hengshui Lake Wetland Reserve in Hebei province, northern China. Colonies of this strain were yellow and cells were rod-shaped, polar-flagellated and obligately aerobic, exhibiting negative Gram reaction. The strain was able to grow at 0-1 % (w/v) NaCl, pH 5-10 and 20-35 °C, with optimal growth occurring at pH 7.0 and 28 °C without NaCl. Chemotaxonomic data revealed that strain WHSC-8T possesses ubiquinone Q-10 as the predominant respiratory quinone, C18 : 1ω7c, C16 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) as the major fatty acids, and sym-homospermidine as the major polyamine. Sphingomonadaceae-specific sphingoglycolipid was detected in the polar lipid patterns. The G+C content of the genomic DNA was 68.7 mol%. All of the above characters corroborated the assignment of the novel strain to the genus Sphingomonas. Strain WHSC-8T shared less than 97.0 % 16S rRNA gene sequence similarity with the type strains of other species of the genus Sphingomonas, except for Sphingomonas asaccharolytica DSM 10564T (97.5 %). The low DNA-DNA relatedness value and distinct phenotypic and chemotaxonomic characteristics distinguished strain WHSC-8T from closely related species of the genus Sphingomonas. Therefore, strain WHSC-8T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas hengshuiensis sp. nov. is proposed. The type strain is WHSC-8T ( = KCTC 42455T = CCTCC AB 2015265T).
authors.affiliation: College of Life Sciences, Hengshui University, Heping West Road 1088, Taocheng District, Hengshui 053000, Hebei, PR China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Wei
authors.affiliation: College of Life Sciences, Hengshui University, Heping West Road 1088, Taocheng District, Hengshui 053000, Hebei, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Shandong Provincial Research Center for Bioinformatic Engineering and Technique, School of Life Sciences, Shandong University of Technology, Zibo 255049, Shandong, PR China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Hengshui University, Heping West Road 1088, Taocheng District, Hengshui 053000, Hebei, PR China.
authors.forename: Caifeng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Hengshui University, Heping West Road 1088, Taocheng District, Hengshui 053000, Hebei, PR China.
authors.forename: Jiping
authors.initials: J
authors.lastname: Guo
authors.affiliation: College of Life Sciences, Hengshui University, Heping West Road 1088, Taocheng District, Hengshui 053000, Hebei, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Hengshui University, Heping West Road 1088, Taocheng District, Hengshui 053000, Hebei, PR China.
authors.forename: Kuijing
authors.initials: K
authors.lastname: Liang
authors.affiliation: College of Life Sciences, Hengshui University, Heping West Road 1088, Taocheng District, Hengshui 053000, Hebei, PR China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006028:Glycosphingolipids / Q000737:chemistry; D060106:Lakes; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013095:Spermidine / Q000031:analogs & derivatives / Q000737:chemistry; D020578:Sphingomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000737:chemistry; D053833:Wetlands
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006028:Glycosphingolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; C007961:sym-homospermidine; D014451:Ubiquinone; C025204:Ubiquinone Q2; D013095:Spermidine