title: Kinetic analysis of decreased sperm fertilizing ability by fluorides and fluoroaluminates: a tool for analyzing the effect of environmental substances on biological events.
abstract: Fluorides and fluoroaluminates decrease mouse sperm fertilizing potential by modifying the process of sperm preparation for fertilization, so-called capacitation, followed by acrosome reaction (AR). Capacitation was monitored by protein tyrosine phosphorylation (pTyr), and AR was induced consequently. The aim of this study was to apply kinetic analysis to the previously obtained dependences of pTyr and AR at capacitation times, and propose a mathematical theory for a mechanism when sperm maturation ability is amended by external stimuli. The experimental input data, previously obtained, are consistent with the proposed theory and the results of kinetic analysis show that sperm capacitation runs as two subsequent first-order steps. Firstly, an unstable intermediate is formed and then gradually decomposes. The time corresponding to the maximal production of the unstable intermediate is probably most suitable for sperm obtaining the ability to fertilize the egg. The presented calculations indicate that the application of kinetic analysis can serve as a tool to predict or confirm a course of biological events that are modified by external factors, and therefore the proposed theory shall be of interest to a broad scientific audience.
authors.affiliation: Department of Analytical Chemistry, Faculty of Science, Charles University, Albertov 2030, 128 43, Prague 2, Czech Republic. bosakova@natur.cuni.cz.
authors.forename: Zuzana
authors.identifier: http://orcid.org/0000-0002-8483-5739
authors.initials: Z
authors.lastname: Bosakova
authors.affiliation: Department of Analytical Chemistry, Faculty of Science, Charles University, Albertov 2030, 128 43, Prague 2, Czech Republic.
authors.forename: Antonin
authors.initials: A
authors.lastname: Tockstein
authors.affiliation: Department of Analytical Chemistry, Faculty of Science, Charles University, Albertov 2030, 128 43, Prague 2, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Adamusova
authors.affiliation: Department of Analytical Chemistry, Faculty of Science, Charles University, Albertov 2030, 128 43, Prague 2, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Coufal
authors.affiliation: Department of Cell Biology, Faculty of Science, Charles University, Vinicna 7, 128 44, Prague 2, Czech Republic.|Laboratory of Reproductive Biology, Institute of Biotechnology AS CR, v.v.i., Videnska 1083, 14220, Prague 4, Czech Republic.
authors.forename: Natasa
authors.initials: N
authors.lastname: Sebkova
authors.affiliation: Biocev Group, Department of Zoology, Faculty of Science, Charles University, Vinicna 7, 128 44, Prague 2, Czech Republic. kathortova@gmail.com.|Laboratory of Reproductive Biology, Institute of Biotechnology AS CR, v.v.i., Videnska 1083, 14220, Prague 4, Czech Republic. kathortova@gmail.com.
authors.forename: Katerina
authors.initials: K
authors.lastname: Dvorakova-Hortova
mesh_terms: D020101:Acrosome Reaction; D000535:Aluminum / Q000009:adverse effects / Q000494:pharmacology*; D000818:Animals; D005459:Fluorides / Q000009:adverse effects / Q000494:pharmacology*; D005461:Fluorine / Q000009:adverse effects / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013079:Sperm Maturation; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
chemical_list: C053629:fluoroaluminum; D005461:Fluorine; D000535:Aluminum; D005459:Fluorides
keywords: Acrosome reaction; Capacitation; Fluoride complexes; Kinetics; Sperm fertilizing ability; Tyrosine phosphorylation