title: Laser ablation ICP-MS: Application in biomedical research.
abstract: In the last decade, the development of diverse bioanalytical methodologies based on mass spectrometry imaging has increased, as has their application in biomedical questions. The distribution analysis of elements (metals, semimetals, and non-metals) in biological samples is a point of interest in life sciences, especially within the context of metallomics, which is the scientific field that encompasses the global analysis of the entirety of elemental species inside a cell or tissue. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been efficiently employed to generate qualitative and quantitative maps of elemental distribution in thin tissue sections of a variety of biological samples, for example, brain, cartilage, spinal cord, etc. The combination of elemental with molecular mass spectrometry allows obtaining information about the elements bound to proteins, when they are previously separated by gel electrophoresis (metalloproteomics), and also adding a new dimension to molecular mass spectrometry imaging by the correlation of molecular and elemental distribution maps in definite regions in a biological tissue. In the present review, recent biomedical applications in LA-ICP-MS imaging as a stand-alone technique and in combination with molecular mass spectrometry imaging techniques are discussed. Applications of LA-ICP-MS in the study of neurodegenerative diseases, distribution of contrast agents and metallodrugs, and metalloproteomics will be focused in this review. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 36:47-57, 2017.
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, Universidade Estadual de Campinas, P.O. Box 6154, 13083-970, Campinas, SP, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Sussulini
authors.affiliation: Maschweg 86, D-33790, Halle/Westfalia, Germany.
authors.forename: Julia Susanne
authors.initials: JS
authors.lastname: Becker
authors.affiliation: Zentralinstitut für Engineering, Elektronik und Analytik, Analytik (ZEA-3), Forschungszentrum Jülich, D-52425, Jülich, Germany.
authors.forename: Johanna Sabine
authors.initials: JS
authors.lastname: Becker
mesh_terms: D000818:Animals; D035843:Biomedical Research / Q000379:methods; D001923:Brain Chemistry; D001927:Brain Diseases / Q000000981:diagnostic imaging*; D003287:Contrast Media / Q000032:analysis*; D006801:Humans; D053685:Laser Therapy; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008670:Metals / Q000032:analysis; D057054:Molecular Imaging / Q000379:methods; D040901:Proteomics / Q000379:methods
chemical_list: D003287:Contrast Media; D008670:Metals
keywords: brain diseases; imaging mass spectrometry; laser ablation inductively coupled plasma mass spectrometry; metallomics; molecular mass spectrometry