title: The extracellular matrix modulates H2O2 degradation and redox signaling in endothelial cells.
abstract: The molecular processes that are crucial for cell function, such as proliferation, migration and survival, are regulated by hydrogen peroxide (H2O2). Although environmental cues, such as growth factors, regulate redox signaling, it was still unknown whether the ECM, a component of the cell microenvironment, had a function in this process. Here, we showed that the extracellular matrix (ECM) differently regulated H2O2 consumption by endothelial cells and that this effect was not general for all types of cells. The analysis of biophysical properties of the endothelial cell membrane suggested that this modification in H2O2 consumption rates was not due to altered membrane permeability. Instead, we found that the ECM regulated GPx activity, a known H2O2 scavenger. Finally, we showed that the extent of PTEN oxidation was dependent on the ECM, indicating that the ECM was able to modulate H2O2-dependent protein oxidation. Thus, our results unraveled a new mechanism by which the ECM regulates endothelial cell function by altering redox balance. These results pinpoint the ECM as an important component of redox-signaling.
authors.affiliation: Centro de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal. Electronic address: anabagulho@gmail.com.
authors.forename: Ana
authors.initials: A
authors.lastname: Bagulho
authors.affiliation: Centro de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal. Electronic address: favilas-boas@fc.ul.pt.
authors.forename: Filipe
authors.initials: F
authors.lastname: Vilas-Boas
authors.affiliation: Centro de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal. Electronic address: andreia.areosapena@gmail.com.
authors.forename: Andreia
authors.initials: A
authors.lastname: Pena
authors.affiliation: Centro de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal. Electronic address: catarinasapeneda@gmail.com.
authors.forename: Catarina
authors.initials: C
authors.lastname: Peneda
authors.affiliation: Centro de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal. Electronic address: fpsantos@fc.ul.pt.
authors.forename: Filipa C
authors.initials: FC
authors.lastname: Santos
authors.affiliation: Centro de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal. Electronic address: ajeronimosousa@gmail.com.
authors.forename: Ana
authors.initials: A
authors.lastname: Jerónimo
authors.affiliation: Centro de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal. Electronic address: rfalmeida@fc.ul.pt.
authors.forename: Rodrigo F M
authors.initials: RFM
authors.lastname: de Almeida
authors.affiliation: Centro de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal. Electronic address: csafonso@fc.ul.pt.
authors.forename: Carla
authors.initials: C
authors.lastname: Real
mesh_terms: D002374:Catalase / Q000378:metabolism; D002448:Cell Adhesion; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D051059:PTEN Phosphohydrolase / Q000378:metabolism
chemical_list: D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: Endothelial cell; Extracellular matrix; Glutathione peroxidase; Hydrogen peroxide; PTEN; Redox signaling