title: Lectin Histochemistry Shows WGA, PHA-L and HPA Binding Increases During Progression of Human Colorectal Cancer.
abstract: OBJECTIVE
  Most colorectal carcinomas develop from an adenoma-carcinoma sequence to metastatic disease. The aim of the present study was to use lectins, carbohydrate-binding proteins, to detect changes in glycosylation during this malignant progression.
  
  
  METHODS
  Sections from normal colorectal mucosa, high-grade intraepithelial neoplasia, submucosal colorectal carcinoma and metastases from patients who underwent colorectal surgery were stained by lectins with different sugar specificities namely agglutins from Wheatgerm (WGA), Helix pomatia (HPA), Phaseolus vulgaris (PHA-L), Ulex europaeus (UEA-I), Sambucus nigra (SNA-I), Canavalia ensiformis (Con A), Galanthus nivalis (GNA) and Dolichos biflorus (DBA).
  
  
  RESULTS
  Binding patterns of all lectins except SNA-I, Con A and DBA changed during the adenoma-carcinoma sequence.
  
  
  CONCLUSIONS
  The results indicate that lectins specific for mannose, N-acetylgalactosamine, N-acetylglucosamine, sialic acid, β-1,6-branched oligosaccharides and α-1-fucose may be associated with malignant progression.
authors.affiliation: Institute of Anatomy and Experimental Morphology, University Hospital Hamburg-Eppendorf, Hamburg, Germany p.haegerbaeumer@gmx.de.
authors.forename: Pia
authors.initials: P
authors.lastname: Hägerbäumer
authors.affiliation: Institute of Pathology, Bayreuth Hospital, Bayreuth, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Vieth
authors.affiliation: Department of Interdisciplinary Endoscopy, University Hospital Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Mario
authors.initials: M
authors.lastname: Anders
authors.affiliation: Institute of Anatomy and Experimental Morphology, University Hospital Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Udo
authors.initials: U
authors.lastname: Schumacher
mesh_terms: D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D018450:Disease Progression; D006031:Glycosylation; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D037102:Lectins
keywords: Adenoma–carcinoma sequence; colon cancer; glycosylation; lectins; malignant progression; metastasis