title: Nickel nanoparticle-modified electrode for ultra-sensitive electrochemical detection of insulin.
abstract: An ultra-sensitive electrochemical sensor for the detection of insulin was fabricated, using low-cost and environmentally friendly nickel nanoparticles (NiNPs) by ion implantation. The morphology and structure of the NiNPs are characterized by scanning electron microscopy (SEM), revealing diameters ranging from 4 to 8 nm. The insulin assay performances were evaluated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (I-t). The NiNPs-modified indium tin oxide electrode (NiNPs/ITO) showed excellent analytical features, including ultra-high sensitivity (2140 μAμM(-1)) for detecting low concentrations of insulin, an incredibly low detection limit (10 pM) and a wide dynamic range (100 pM to 2400 pM and 1 nM to 125 nM). In addition, the NiNPs/ITO electrode was also used to analyze the insulin concentration in bovine insulin injections. The NiNPs/ITO electrode is expected to be used as a potential biosensor for insulin.
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Meisong
authors.initials: M
authors.lastname: Guo
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Mengwei
authors.initials: M
authors.lastname: Yuan
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Weitong
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing 100875, PR China; Key Laboratory of Beam Technology and Material Modification of Ministry of Education, Beijing Normal University, Beijing 100875, PR China. Electronic address: 94021@bnu.edu.cn.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Hu
mesh_terms: D000327:Adsorption; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007328:Insulin / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008832:Microchemistry / Q000295:instrumentation*; D008839:Microelectrodes; D009532:Nickel / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D007328:Insulin; D017971:Tin Compounds; C109984:indium tin oxide; D009532:Nickel
keywords: Electrochemical detection; Insulin; Ion implantation; Nickel nanoparticles