title: MicroRNAs in urine are not biomarkers of multiple myeloma.
abstract: BACKGROUND
  In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease.
  
  
  RESULTS
  Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays for qPCR. We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them. Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance.
  
  
  CONCLUSIONS
  Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma.
authors.affiliation: Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. lena.sedlarikova@gmail.com.|Department of Clinical Hematology, University Hospital Brno, Brno, Czech Republic. lena.sedlarikova@gmail.com.
authors.forename: Lenka
authors.initials: L
authors.lastname: Sedlaříková
authors.affiliation: Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. lenka.kubiczek@gmail.com.|Department of Hematology and Oncology, Kantonsspital St. Gallen, St. Gallen, Switzerland. lenka.kubiczek@gmail.com.
authors.forename: Lenka
authors.initials: L
authors.lastname: Bešše
authors.affiliation: Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. 357892@mail.muni.cz.
authors.forename: Soňa
authors.initials: S
authors.lastname: Novosadová
authors.affiliation: Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. veronika.kubaczkova@seznam.cz.|Department of Clinical Hematology, University Hospital Brno, Brno, Czech Republic. veronika.kubaczkova@seznam.cz.
authors.forename: Veronika
authors.initials: V
authors.lastname: Kubaczková
authors.affiliation: Central European Institute of Technology, Masaryk University, Brno, Czech Republic. avodar@gmail.com.
authors.forename: Lenka
authors.initials: L
authors.lastname: Radová
authors.affiliation: Department of urologic oncology, Masaryk Memorial Cancer Institute, Brno, Czech Republic. stanik@mou.cz.
authors.forename: Michal
authors.initials: M
authors.lastname: Staník
authors.affiliation: Department of Internal Medicine - Hematooncology, University Hospital Brno, Brno, Czech Republic. mkrejci@fnbrno.cz.
authors.forename: Marta
authors.initials: M
authors.lastname: Krejčí
authors.affiliation: Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. rom.hajek@seznam.cz.|Department of Clinical Hematology, University Hospital Brno, Brno, Czech Republic. rom.hajek@seznam.cz.|Department of Hematooncology, Faculty of Medicine University of Ostrava and University Hospital Ostrava, Ostrava, Czech Republic. rom.hajek@seznam.cz.
authors.forename: Roman
authors.initials: R
authors.lastname: Hájek
authors.affiliation: Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. sevcik@med.muni.cz.|Department of Clinical Hematology, University Hospital Brno, Brno, Czech Republic. sevcik@med.muni.cz.
authors.forename: Sabina
authors.initials: S
authors.lastname: Ševčíková
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000652:urine*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000652:urine*; D008875:Middle Aged; D009101:Multiple Myeloma / Q000175:diagnosis* / Q000235:genetics / Q000652:urine*
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs