title: Improving Bone Microarchitecture in Aging with Diosgenin Treatment: A Study in Senescence-Accelerated OXYS Rats.
abstract: Osteoporosis is a major disease associated with aging. We have previously demonstrated that diosgenin prevents osteoporosis in both menopause and D-galactose-induced aging rats. OXYS rats reveal an accelerated senescence and are used as a suitable model of osteoporosis. The aim of the present study was to analyze microarchitecture and morphological changes in femur of OXYS rats using morphological tests and microcomputed tomography scanning, and to evaluate the effects of oral administration of diosgenin at 10 and 50 mg/kg/day on femur in OXYS rats. The result showed that, compared with age-matched Wistar rats, the femur of OXYS rats revealed lower bone length, bone weight, bone volume, frame volume, frame density, void volume, porosity, external and internal diameters, cortical bone area, BV/TV, Tb.N, and Tb.Th, but higher Tb.Sp. Eight weeks of diosgenin treatment decreased porosity and Tb.Sp, but increased BV/TV, cortical bone area, Tb.N and bone mineral density, compared with OXYS rats treated with vehicle. These data reveal that microarchitecture and morphological changes in femur of OXYS rats showed osteoporotic aging features and suggest that diosgenin may have beneficial effects on aging-induced osteoporosis.
authors.affiliation: Laboratory of Evolutionary Genetics, Institute of Cytology and Genetics SB RAS, Novosibirsk 630090, Russia, Taiwan, Republic of China.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Tikhonova
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung 40201, Taiwan, Republic of China.
authors.forename: Che-Hao
authors.initials: CH
authors.lastname: Ting
authors.affiliation: Department of Molecular Mechanisms of Ageing, Institute of Cytology and Genetics SB RAS, Novosibirsk 630090, Russia.
authors.forename: Nataliya G
authors.initials: NG
authors.lastname: Kolosova
authors.affiliation: Division of Urology, Department of Surgery, Tungs' Taichung Metrohabor Hospital, Taichung 43503, Taiwan, Republic of China.
authors.forename: Chao-Yu
authors.initials: CY
authors.lastname: Hsu
authors.affiliation: School of Physical Therapy, Chung Shan Medical University, Taichung 40201, Taiwan, Republic of China.
authors.forename: Jian-Horng
authors.initials: JH
authors.lastname: Chen
authors.affiliation: Molecular Imaging Laboratory, Department of Nuclear Medicine, Chung Shan Medical University Hospital, Taichung 40201, Taiwan, Republic of China.
authors.forename: Chi-Wen
authors.initials: CW
authors.lastname: Huang
authors.affiliation: School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung 40201, Taiwan, Republic of China.
authors.forename: Ging-Ting
authors.initials: GT
authors.lastname: Tseng
authors.affiliation: Occupational Safety and Health Office, Department of Education and Research Taipei City Hospital, Taipei 10341, Taiwan, Republic of China.
authors.forename: Ching-Sui
authors.initials: CS
authors.lastname: Hung
authors.affiliation: Molecular Imaging Laboratory, Department of Nuclear Medicine, Chung Shan Medical University Hospital, Taichung 40201, Taiwan, Republic of China.|School of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan, Republic of China.
authors.forename: Pan-Fu
authors.initials: PF
authors.lastname: Kao
authors.affiliation: Laboratory of Evolutionary Genetics, Institute of Cytology and Genetics SB RAS, Novosibirsk 630090, Russia, Taiwan, Republic of China.
authors.forename: Tamara G
authors.initials: TG
authors.lastname: Amstislavskaya
authors.affiliation: School of Psychology, Chung Shan Medical University Hospital, Chung Shan Medical University Taichung 40201, Taiwan, Republic of China.
authors.forename: Ying-Jui
authors.initials: YJ
authors.lastname: Ho
mesh_terms: D000375:Aging / Q000473:pathology; D000818:Animals; D027742:Dioscorea; D004144:Diosgenin / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005269:Femur / Q000187:drug effects* / Q000473:pathology; D008297:Male; D010024:Osteoporosis / Q000188:drug therapy* / Q000473:pathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts; D004144:Diosgenin