title: Epithelial membrane protein 3 functions as an oncogene and is regulated by microRNA-765 in primary breast carcinoma.
abstract: Epithelial membrane protein 3 (EMP3) is a transmembrane signaling molecule, which is important in the regulation of apoptosis, differentiation and invasion of cancer cells. However, the specific function and regulatory mechanism of EMP3 in primary breast carcinoma remain to be elucidated. In the present study, the mRNA and protein levels of EMP3 were observed to be upregulated in primary breast carcinoma tissues, compared with normal tissues. It was hypothesized that the overexpression of EMP3 was correlated with the downregulation of microRNA‑765 (miR‑765), an underexpressed miRNA in primary breast carcinoma tissues. Functional analysis demonstrated that EMP3 was regulated by miR‑765 through binding to its 3'untranslated region. In addition, the knockdown of EMP3 and miR‑765 had similar effects on the inhibition of proliferation and invasion in SK‑BR‑3 cells. These results provided novel insight into the regulatory mechanism of EMP3 in primary breast carcinoma.
authors.affiliation: Department of Clinical Laboratory, Nantong Cancer Hospital, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Xiao Chun
authors.initials: XC
authors.lastname: Hong
authors.affiliation: Department of Clinical Laboratory, Traditional Chinese Medicine Hospital, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Yuan Jian
authors.initials: YJ
authors.lastname: Fen
authors.affiliation: Department of Clinical Laboratory, Traditional Chinese Medicine Hospital, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Guo Chun
authors.initials: GC
authors.lastname: Yan
authors.affiliation: Department of Clinical Laboratory, Traditional Chinese Medicine Hospital, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Hong
authors.affiliation: Department of Clinical Laboratory, Traditional Chinese Medicine Hospital, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Cao Hong
authors.initials: CH
authors.lastname: Yan
authors.affiliation: Department of Clinical Laboratory, Traditional Chinese Medicine Hospital, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Li Wei
authors.initials: LW
authors.lastname: Bing
authors.affiliation: Department of Clinical Laboratory, Traditional Chinese Medicine Hospital, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Yu Hai
authors.initials: YH
authors.lastname: Zhong
mesh_terms: D020413:3' Untranslated Regions; D000368:Aged; D017209:Apoptosis; D001483:Base Sequence; D001665:Binding Sites; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D008969:Molecular Sequence Data; D009367:Neoplasm Staging; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C485157:EMP3 protein, human; C000600151:MIRN765 microRNA, human; D008562:Membrane Glycoproteins; D035683:MicroRNAs; D012333:RNA, Messenger; D034741:RNA, Small Interfering