title: Selective inhibition of cancer cells' proliferation by compounds included in extracts from Baltic Sea cyanobacteria.
abstract: Cyanobacteria are a rich source of biologically active compounds used in pharmacology and biotechnology. Due to their high capacity of adaptation, which is reflected in the production of diverse metabolites, including toxins, these microorganisms are able to inhabit very different environments. In this work, water and ethanol extracts from 11 cyanobacterial strains derived from the Baltic Sea (Microcystis, Synechocystis, Leptolyngbya, Pseudanabaena, Lyngbya, Phormidium, Nodularia and Anabaena genera) were screened for anticancer activity. MCF-7 human breast cancer and HeLa cervical cancer cell lines, as well as HDFa normal human fibroblasts, were used. Three extracts derived from Pseudanabaena sp., Pseudanabaena cf. galeata and Microcystis aeruginosa revealed potent and selective antiproliferative activities against cancer cells. The mechanism of the anticancer activity was explored in MCF-7 cells, and was found to rely on the inhibition of the pro-survival Akt kinase and induction of cell death. The peptide profiles of selected cyanobacterial extracts were determined using LC-MS/MS, and classes of bioactive compounds that might be potentially responsible for the observed anticancer activities are presented.
authors.affiliation: Department of Molecular Biology, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Felczykowska
authors.affiliation: Department of Molecular Biology, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Pawlik
authors.affiliation: Department of Marine Biotechnology, Institute of Oceanography, University of Gdańsk, Al. Marszałka Piłsudskiego 46, 81-378 Gdynia, Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Mazur-Marzec
authors.affiliation: Department of Marine Biotechnology, Institute of Oceanography, University of Gdańsk, Al. Marszałka Piłsudskiego 46, 81-378 Gdynia, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Toruńska-Sitarz
authors.affiliation: Laboratory of Electron Microscopy, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Narajczyk
authors.affiliation: Laboratory of Electron Microscopy, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors.forename: Malwina
authors.initials: M
authors.lastname: Richert
authors.affiliation: Department of Molecular Biology, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Węgrzyn
authors.affiliation: Department of Molecular Biology, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland. Electronic address: anna.herman-antosiewicz@biol.ug.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Herman-Antosiewicz
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002853:Chromatography, Liquid; D000458:Cyanobacteria / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D008387:Marine Toxins / Q000737:chemistry / Q000494:pharmacology*; D009792:Oceans and Seas; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors; D053719:Tandem Mass Spectrometry
chemical_list: D000970:Antineoplastic Agents; D008387:Marine Toxins; D051057:Proto-Oncogene Proteins c-akt
keywords: Apoptosis; Bioactive compounds; Cancer cells; Marine cyanobacteria; Necrosis; Pseudanabaena