title: Replication-Competent Influenza B Reporter Viruses as Tools for Screening Antivirals and Antibodies.
abstract: Influenza B virus is a human pathogen responsible for significant health and economic burden. Research into this pathogen has been limited by the lack of reporter viruses. Here we describe the development of both a replication-competent fluorescent influenza B reporter virus and bioluminescent influenza B reporter virus. Furthermore, we demonstrate these reporter viruses can be used to quickly monitor viral growth and permit the rapid screening of antiviral compounds and neutralizing antibodies.
authors.affiliation: Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: Benjamin O
authors.initials: BO
authors.lastname: Fulton
authors.affiliation: Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Palese
authors.affiliation: Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA nicholas.heaton@duke.edu.
authors.forename: Nicholas S
authors.initials: NS
authors.lastname: Heaton
mesh_terms: D000818:Animals; D004285:Dogs; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005434:Flow Cytometry; D017930:Genes, Reporter / Q000235:genetics*; D009981:Influenza B virus / Q000235:genetics* / Q000502:physiology; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D061985:Madin Darby Canine Kidney Cells; D008856:Microscopy, Fluorescence; D014779:Virus Replication / Q000502:physiology
chemical_list: D008164:Luminescent Proteins