title: Melamine-DNA encoded periodicity of quantum dot arrays.
abstract: Formation of QD-array in solution phase guided by the self-assembly with DNA-melamine hybrid molecules is reported here. Melamine was conjugated with ssDNA using phosphoramidate chemistry. Aqueous soluble ZnSe/ZnS QDs conjugated to complementary ssDNA was self-assembled with the DNA-melamine hybrid molecules by DNA-hybridization. The self-assembly leads to the precise positioning of the QDs in QDs array where the inter QD distance is being maintained by the DNA sequence length. The QD array was characterized by gel electrophoresis, UV-visible and fluorescence spectrophotometry and circular dichroism. Direct visualization of the DNA-melamine hybrid molecule mediated QD array was made possible by atomic force microscopy (AFM) and transmission electron microscopy (TEM) analysis. Substantial increase in the fluorescence intensity and lifetime of the QDs was observed on array formation by DNA self-assembly.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Patna, Patna 800013, Bihar, India.
authors.forename: Seema
authors.initials: S
authors.lastname: Singh
authors.affiliation: Department of Chemistry, Indian Institute of Technology Patna, Patna 800013, Bihar, India.
authors.forename: Rina
authors.initials: R
authors.lastname: Kumari
authors.affiliation: Department of Chemistry, Indian Institute of Technology Patna, Patna 800013, Bihar, India.
authors.forename: Anirban
authors.initials: A
authors.lastname: Chakraborty
authors.affiliation: Department of Chemistry, Indian Institute of Technology Patna, Patna 800013, Bihar, India.
authors.forename: Sahid
authors.initials: S
authors.lastname: Hussain
authors.affiliation: Atomic and Molecular Physics Division, Bhabha Atomic Research Centre, Mumbai 400085, India. Electronic address: mksingh@barc.gov.in.
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Singh
authors.affiliation: Department of Chemistry, Indian Institute of Technology Patna, Patna 800013, Bihar, India. Electronic address: prolay@iitp.ac.in.
authors.forename: Prolay
authors.initials: P
authors.lastname: Das
mesh_terms: D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry*; D010316:Particle Size; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D013499:Surface Properties; D014227:Triazines / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D004277:DNA, Single-Stranded; D018036:Selenium Compounds; D013440:Sulfides; D014227:Triazines; D017967:Zinc Compounds; C031238:zinc sulfide; C011907:melamine; C044696:zinc selenide
keywords: DNA–organic hybrid; Melamine; Quantum dot; Quantum dot arrays; Self-assembly