title: [Osthole ameliorates glutamate-induced toxicity in HT22 cells via activating PI3K/Akt signaling pathway].
abstract: OBJECTIVE
  To investigate the neuroprotective effects of osthole (OST) on glutamate-induced toxicity in hippocampal HT22 cells and to explore the correlation between the protection and phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) signaling pathway.
  
  
  METHODS
  The cell injury model of HT22 was induced by glutamate and the cell viability was detected by MTS assay. The lactate dehydrogenase (LDH) release and the caspase-3 activity were determined by commercial kits. Western blot analysis was utilized to detect the protein levels of PI3K, Akt, p-PI3K and p-Akt.
  
  
  RESULTS
  OST markedly improved the cell survival and decreased the LDH release in glutamate-treated HT22 cells in a dose-dependent manner. Furthermore, the levels of p-PI3K and p-Akt proteins were significantly increased in glutamate and OST-co-treated HT22 cells. The effect of OST on p-Akt phosphorylation in HT22 cells was attenuated in the presence of PI3K specific inhibitor (LY294002).
  
  
  CONCLUSIONS
  OST protects HT22 cells from glutamate excitotoxicity through a mechanism involving the activation of PI3K/Akt signaling pathway.
authors.affiliation: Institute of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008; Institute of Clinical Pharmacology; Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Mao
authors.affiliation: Institute of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008; Institute of Clinical Pharmacology; Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008; Institute of Clinical Pharmacology; Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, China.
authors.forename: Honghao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Institute of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008; Institute of Clinical Pharmacology; Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, China.
authors.forename: Zhaoqian
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Orthopeadics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival; D002867:Chromones / Q000494:pharmacology; D003374:Coumarins / Q000494:pharmacology*; D018698:Glutamic Acid / Q000009:adverse effects*; D006624:Hippocampus / Q000166:cytology; D051379:Mice; D009025:Morpholines / Q000494:pharmacology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D002867:Chromones; D003374:Coumarins; D009025:Morpholines; D018696:Neuroprotective Agents; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D018698:Glutamic Acid; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; C505409:Casp3 protein, mouse; D053148:Caspase 3; C046627:osthol