title: Involvement of miR528 in the Regulation of Arsenite Tolerance in Rice (Oryza sativa L.).
abstract: Tens of miRNAs were previously established as being arsenic (As) stress responsive in rice. However, their functional role in As tolerance remains unclear. This study demonstrates that transgenic plants overexpressing miR528 (Ubi::MIR528) were more sensitive to arsenite [As(III)] compared with wild-type (WT) rice. Under normal and stress conditions, miR528-5p and -3p were highly up-regulated in both the roots and leaves of transgenic plants, which exhibited a negative correlation with the expression of seven target genes. Compared with WT plants, Ubi::MIR528 plants showed excessive oxidative stress generation and remarkable amino acid content changes in the roots and leaves upon As(III) exposure. Notably, the expression profiles of diverse functional genes were clearly different between WT and transgenic plants. Thus, the observed As(III) sensitivity of Ubi::MIR528 plants was likely due to the strong alteration of antioxidant enzyme activity and amino acid profiles and the impairment of the As(III) uptake, translocation, and tolerance systems of rice.
authors.forename: Qingpo
authors.initials: Q
authors.lastname: Liu
authors.forename: Haichao
authors.initials: H
authors.lastname: Hu
authors.forename: Leyi
authors.initials: L
authors.lastname: Zhu
authors.forename: Ruochen
authors.initials: R
authors.lastname: Li
authors.affiliation: College of Environmental and Resources Science, Zhejiang University , Hangzhou 310058, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.forename: Liqing
authors.initials: L
authors.lastname: Zhang
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Yang
authors.forename: Xingquan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences , Hangzhou 310021, People's Republic of China.
authors.forename: Hengmu
authors.initials: H
authors.lastname: Zhang
mesh_terms: D018053:Arsenites / Q000378:metabolism*; D001692:Biological Transport; D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*
chemical_list: D018053:Arsenites; D035683:MicroRNAs; D010940:Plant Proteins; D018749:RNA, Plant; C015001:arsenite
keywords: arsenite tolerance; gene expression pattern; miR528; overexpression; rice