title: Effects of heat and drought stress on post-illumination bursts of volatile organic compounds in isoprene-emitting and non-emitting poplar.
abstract: Over the last decades, post-illumination bursts (PIBs) of isoprene, acetaldehyde and green leaf volatiles (GLVs) following rapid light-to-dark transitions have been reported for a variety of different plant species. However, the mechanisms triggering their release still remain unclear. Here we measured PIBs of isoprene-emitting (IE) and isoprene non-emitting (NE) grey poplar plants grown under different climate scenarios (ambient control and three scenarios with elevated CO2 concentrations: elevated control, periodic heat and temperature stress, chronic heat and temperature stress, followed by recovery periods). PIBs of isoprene were unaffected by elevated CO2 and heat and drought stress in IE, while they were absent in NE plants. On the other hand, PIBs of acetaldehyde and also GLVs were strongly reduced in stress-affected plants of all genotypes. After recovery from stress, distinct differences in PIB emissions in both genotypes confirmed different precursor pools for acetaldehyde and GLV emissions. Changes in PIBs of GLVs, almost absent in stressed plants and enhanced after recovery, could be mainly attributed to changes in lipoxygenase activity. Our results indicate that acetaldehyde PIBs, which recovered only partly, derive from a new mechanism in which acetaldehyde is produced from methylerythritol phosphate pathway intermediates, driven by deoxyxylulose phosphate synthase activity.
authors.affiliation: Institute of Ion and Applied Physics, University of Innsbruck, 6020, Innsbruck, Austria.
authors.forename: Werner
authors.identifier: 0000-0003-4267-0435
authors.initials: W
authors.lastname: Jud
authors.affiliation: Research Unit Environmental Simulation (EUS), Institute of Biochemical Plant Pathology (BIOP), Helmholtz Zentrum München GmbH, 85764, Neuherberg, Germany.
authors.forename: Elisa
authors.initials: E
authors.lastname: Vanzo
authors.affiliation: Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing Michigan, 48823, USA.
authors.forename: Ziru
authors.initials: Z
authors.lastname: Li
authors.affiliation: Research Unit Environmental Simulation (EUS), Institute of Biochemical Plant Pathology (BIOP), Helmholtz Zentrum München GmbH, 85764, Neuherberg, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ghirardo
authors.affiliation: Research Unit Environmental Simulation (EUS), Institute of Biochemical Plant Pathology (BIOP), Helmholtz Zentrum München GmbH, 85764, Neuherberg, Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Zimmer
authors.affiliation: Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing Michigan, 48823, USA.
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Sharkey
authors.affiliation: Institute of Ion and Applied Physics, University of Innsbruck, 6020, Innsbruck, Austria.
authors.forename: Armin
authors.initials: A
authors.lastname: Hansel
authors.affiliation: Research Unit Environmental Simulation (EUS), Institute of Biochemical Plant Pathology (BIOP), Helmholtz Zentrum München GmbH, 85764, Neuherberg, Germany.
authors.forename: Jörg-Peter
authors.identifier: 0000-0002-9825-867X
authors.initials: JP
authors.lastname: Schnitzler
mesh_terms: D002070:Butadienes / Q000378:metabolism*; D003681:Dehydration; D045782:Hemiterpenes / Q000378:metabolism*; D006358:Hot Temperature; D008084:Lipoxygenase / Q000378:metabolism; D010420:Pentanes / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects; D018526:Plant Transpiration / Q000502:physiology / Q000528:radiation effects; D032107:Populus / Q000378:metabolism* / Q000502:physiology / Q000528:radiation effects; D055549:Volatile Organic Compounds / Q000378:metabolism*
chemical_list: D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; D055549:Volatile Organic Compounds; C005059:isoprene; D008084:Lipoxygenase
keywords: LOX; Populus x canescens; VOC; acetaldehyde; climate change scenarios; green leaf volatiles; grey poplar; isoprene; post-illumination bursts