title: Structure and Sialyllactose Binding of the Carboxy-Terminal Head Domain of the Fibre from a Siadenovirus, Turkey Adenovirus 3.
abstract: The virulent form of turkey adenovirus 3 (TAdV-3), also known as turkey hemorrhagic enteritis virus (THEV), is an economically important poultry pathogen, while the avirulent form is used as a vaccine. TAdV-3 belongs to the genus Siadenovirus. The carboxy-terminal region of its fibre does not have significant sequence similarity to any other adenovirus fibre heads of known structure. Two amino acid sequence differences between virulent and avirulent TAdV-3 map on the fibre head: where virulent TAdV-3 contains Ile354 and Thr376, avirulent TAdV-3 contains Met354 and Met376. We determined the crystal structures of the trimeric virulent and avirulent TAdV-3 fibre head domains at 2.2 Å resolution. Each monomer contains a beta-sandwich, which, surprisingly, resembles reovirus fibre head more than other adenovirus fibres, although the ABCJ-GHID topology is conserved in all. A beta-hairpin insertion in the C-strand of each trimer subunit embraces its neighbouring monomer. The avirulent and virulent TAdV-3 fibre heads are identical apart from the exact orientation of the beta-hairpin insertion. In vitro, sialyllactose was identified as a ligand by glycan microarray analysis, nuclear magnetic resonance spectroscopy, and crystallography. Its dissociation constant was measured to be in the mM range by isothermal titration calorimetry. The ligand binds to the side of the fibre head, involving amino acids Glu392, Thr419, Val420, Lys421, Asn422, and Gly423 binding to the sialic acid group. It binds slightly more strongly to the avirulent form. We propose that, in vivo, the TAdV-3 fibre may bind a sialic acid-containing cell surface component.
authors.affiliation: Departamento de Estructura de Macromoléculas, Centro Nacional de Biotecnología (CNB-CSIC), Madrid, Spain.
authors.forename: Abhimanyu K
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Departamento de Biología Física-Química, Centro de Investigaciones Biológicas (CIB-CSIC), Madrid, Spain.
authors.forename: M Álvaro
authors.initials: MÁ
authors.lastname: Berbís
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Mónika Z
authors.initials: MZ
authors.lastname: Ballmann
authors.affiliation: Glycoscience Group, National Centre for Biomedical Engineering Science, National University of Ireland Galway, Galway, Ireland; Microbiology, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
authors.forename: Michelle
authors.initials: M
authors.lastname: Kilcoyne
authors.affiliation: Departamento de Química Física-Biológica, Instituto de Química Física Rocasolano (IQFR-CSIC) and CIBER de Enfermedades Respiratorias (CIBERES), calle Serrano 119, E-28006 Madrid, Spain.
authors.forename: Margarita
authors.initials: M
authors.lastname: Menéndez
authors.affiliation: Departamento de Estructura de Macromoléculas, Centro Nacional de Biotecnología (CNB-CSIC), Madrid, Spain.
authors.forename: Thanh H
authors.initials: TH
authors.lastname: Nguyen
authors.affiliation: Glycoscience Group, National Centre for Biomedical Engineering Science, National University of Ireland Galway, Galway, Ireland.
authors.forename: Lokesh
authors.initials: L
authors.lastname: Joshi
authors.affiliation: Departamento de Biología Física-Química, Centro de Investigaciones Biológicas (CIB-CSIC), Madrid, Spain.
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Cañada
authors.affiliation: Departamento de Biología Física-Química, Centro de Investigaciones Biológicas (CIB-CSIC), Madrid, Spain; Centro de Investigación Cooperativa en Biociencias (CIC bioGUNE), Parque Tecnológico de Bizkaia, Derio, Spain; Ikerbasque, Basque Foundation for Science, Bilbao, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Jiménez-Barbero
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Mária
authors.initials: M
authors.lastname: Benkő
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Harrach
authors.affiliation: Departamento de Estructura de Macromoléculas, Centro Nacional de Biotecnología (CNB-CSIC), Madrid, Spain.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: van Raaij
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D002151:Calorimetry / Q000379:methods; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D018360:Crystallography, X-Ray; D007785:Lactose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D046809:Siadenovirus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D012794:Sialic Acids / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014774:Virulence / Q000235:genetics
chemical_list: D011134:Polysaccharides; D012794:Sialic Acids; D014764:Viral Proteins; C000020:N-acetylneuraminoyllactose; D007785:Lactose