title: Radiations and biodegradation techniques for detoxifying Carica papaya seed oil for effective dietary and industrial use.
abstract: Benzyl isothiocyanate (BITC) is toxic in high concentration. The capacity of Aspergillus niger, microwave and ultraviolet radiations to reduce the BITC levels in Carica papaya Linn seed oil were assessed in vitro. BITC at different concentrations were periodically exposed to microwave and ultraviolet radiations for 30 min and 10 h, respectively; and to identify Aspergillus niger for 4 days. Microwave radiation significantly reduced (p < 0.05) BITC levels (0.0272, 0.0544, and 0.0816 μmol) to 12.19, 8.99 and 27.5 % respectively within 15 min. Ultraviolet radiation significantly reduced (p < 0.05) BITC levels at all the concentrations. A. niger significantly increased (p < 0.05) BITC degradation on days 2 and 4 at 0.816, 1.36 and 2.72 nmol. Glutathione activity was significantly increased (p < 0.05) while glutathione S-transferase activity significantly reduced (p < 0.05) at all concentrations on days 3 and 4 respectively. The three techniques are possible models for improving the dietary consumption of the oil.
authors.affiliation: College of Science and Technology, Biological Sciences Department, Biochemistry Unit, Covenant University, Canaan Land, Km. 10, Idiroko Road, P.M.B. 1023, Ota, Ogun State Nigeria.
authors.forename: Israel Sunmola
authors.initials: IS
authors.lastname: Afolabi
authors.affiliation: College of Science and Technology, Biological Sciences Department, Biochemistry Unit, Covenant University, Canaan Land, Km. 10, Idiroko Road, P.M.B. 1023, Ota, Ogun State Nigeria.
authors.forename: Tolulope Dorcas
authors.initials: TD
authors.lastname: Bisi-Adeniyi
authors.affiliation: College of Science and Technology, Biological Sciences Department, Biochemistry Unit, Covenant University, Canaan Land, Km. 10, Idiroko Road, P.M.B. 1023, Ota, Ogun State Nigeria.
authors.forename: Toluwalase Ronke
authors.initials: TR
authors.lastname: Adedoyin
authors.affiliation: College of Science and Technology, Biological Sciences Department, Biochemistry Unit, Covenant University, Canaan Land, Km. 10, Idiroko Road, P.M.B. 1023, Ota, Ogun State Nigeria.
authors.forename: Solomon Oladapo
authors.initials: SO
authors.lastname: Rotimi
keywords: Aspergillus niger; Benzyl isothiocyanate; Carica papaya seed; Microwave; Ultraviolet light