title: Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction.
abstract: BACKGROUND
  Invasion of host cells by Trypanosoma cruzi extracellular amastigotes is host actin polymerization-dependent. However, the role of proteins related to actin dynamics during invasion by amastigotes remains to be investigated. Here we describe the role of Annexin A2 and ARF-6 during extracellular amastigote-mammalian cell interactions.
  
  
  RESULTS
  Our results showed ARF-6 accumulation in the amastigote-containing parasitophorous vacuole containing amastigote forms; demonstrated ARF-6 and Annexin A2 critical impact over parasite cell invasion and revealed the effect of Annexin A2 expression on intracellular parasite multiplication.
  
  
  CONCLUSIONS
  ARF-6 and Annexin A2 are involved in invasion of mammalian cells by T. cruzi amastigotes.
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Brasil. thaiselara@yahoo.com.br.
authors.forename: Thaise Lara
authors.initials: TL
authors.lastname: Teixeira
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Brasil. lilianlc@gmail.com.
authors.forename: Lilian
authors.initials: L
authors.lastname: Cruz
authors.affiliation: Universidade Federal de São Paulo, São Paulo, Brazil. ramortara@unifesp.br.
authors.forename: Renato Arruda
authors.initials: RA
authors.lastname: Mortara
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Brasil. silva_cv@yahoo.com.br.|Laboratório de Tripanosomatídeos, Disciplina de Imunologia - Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Rua Piauí, Bloco 2B sala 200, Campus Umuarama, Uberlândia, MG, 38400-902, Brasil. silva_cv@yahoo.com.br.
authors.forename: Claudio Vieira
authors.initials: CV
authors.lastname: Da Silva
mesh_terms: D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017306:Annexin A2 / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D017353:Gene Deletion; D005786:Gene Expression Regulation / Q000502:physiology; D051379:Mice; D014349:Trypanosoma cruzi / Q000502:physiology*
chemical_list: D000089982:ADP-Ribosylation Factor 6; D017306:Annexin A2; D020727:ADP-Ribosylation Factors; C000715727:Arf6 protein, mouse