title: Spent coffee ground extract suppresses ultraviolet B-induced photoaging in hairless mice.
abstract: The aim of this study is to evaluate the effect of spent coffee ground (SCG) ethanol extract on UVB-induced skin aging in hairless mice. An ethanol extract of SCG (ESCG) was prepared using the residue remaining after extraction of oil from roasted SCG. High performance liquid chromatography (HPLC) analysis showed that the content of caffeine (41.58 ± 0.54 μg/mg) was higher than that of chlorogenic acid isomers (~9.17 μg/mg) in ESCG. ESCG significantly decreased the UVB-induced intracellular reactive oxygen species in HaCaT cells. UVB-induced wrinkle formation in mice dorsal skin was effectively reduced by ESCG administration; high dose of ESCG (5 g/L) caused the reduction of wrinkle area by 30% compared with UVB-treated control (UVBC). This result correlated with the ESCG-mediated decrease in epidermis thickness (25%). In addition, ESCG administration significantly reduced transdermal water loss (20%) and erythema formation (35%) derived from UVB exposure. Collagen type I (COL-1) level in dorsal skin was effectively recovered by ESCG administration. These results were supported by down-regulation of collagen-degrading matrix metalloproteinase 2 (MMP2) and 9 (MMP9) expressions. Our results indicate that ESCG protects mouse skin from UVB-induced photoaging by suppressing the expression of matrix metalloproteinases. Our study suggests that ESCG may be anti-photoaging agent.
authors.affiliation: Department of Food Science and Technology, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Hyeon-Son
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Kwang Dong Pharmaceutical Co., LTD., Gyeonggi-do 459-040, Republic of Korea.
authors.forename: Eu Ddeum
authors.initials: ED
authors.lastname: Park
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Yooheon
authors.initials: Y
authors.lastname: Park
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul 136-713, Republic of Korea. Electronic address: suh1960@korea.ac.kr.
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Suh
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003069:Coffee / Q000737:chemistry* / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D003069:Coffee; D024042:Collagen Type I; D010936:Plant Extracts; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: HaCaT cell; Hairless mouse; ROS scavenging activity; Spent coffee ground; UVB-induced photoaging