title: A PER3 polymorphism is associated with better overall survival in diffuse large B-cell lymphoma in Mexican population.
abstract: BACKGROUND
  Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of malignant lymphoma. Presently, one of the most important clinical predictors of survival in DLBCL patients is the International Prognostic Index (IPI). Circadian rhythms are the approximate 24 hour biological rhythms with more than 10 genes making up the molecular clock.
  
  
  OBJECTIVE
  Determine if functional single nucleotide polymorphism in circadian genes may contribute to survival status in patients diagnosed with diffuse large B-cell lymphoma.
  
  
  METHODS
  Sixteen high-risk non-synonymous polymorphisms in circadian genes (CLOCK, CRY2, CSNK1E, CSNK2A1, NPAS2, PER1, PER2, PER3, PPP2CA, and TIM) were genotyped by screening PCR. Results were visualized by agarose gel electrophoresis and confirmed by two-direction sequencing. Clinical variables were compared between mutated and non-mutated groups. LogRank survival analysis and Kaplan-Meier method were used to calculate the overall survival.
  
  
  RESULTS
  PER3 rs10462020 variant showed significant difference in overall survival between patients containing mutated genotypes and those with non-mutated genotypes (p = 0.047). LDH levels (p = 0.021) and IPI score (p < 0.001) also showed differences in overall survival. No clinical differences were observed in mutated vs. non-mutated patients.
  
  
  CONCLUSIONS
  This work suggests a role of PER3 rs10462020 in predicting a prognosis in DLBCL overall survival of patients.
authors.affiliation: Tecnológico de Monterrey, Escuela Nacional de Medicina, Monterrey, Nuevo León, México.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Gutiérrez-Monreal
authors.affiliation: Tecnológico de Monterrey, Escuela Nacional de Medicina, Monterrey, Nuevo León, México.
authors.forename: Luis
authors.initials: L
authors.lastname: Villela
authors.affiliation: Servicio de Hematología, Unidad Médica de Alta Especialidad IMSS No.25, Monterrey, Nuevo León, México.
authors.forename: Severiano
authors.initials: S
authors.lastname: Baltazar
authors.affiliation: Tecnológico de Monterrey, Escuela Nacional de Medicina, Monterrey, Nuevo León, México.
authors.forename: Yocanxochitl
authors.initials: Y
authors.lastname: Perfecto-Avalos
authors.affiliation: Tecnológico de Monterrey, Centro de Agrobiotecnología, Monterrey, Nuevo León, México.
authors.forename: Guy A
authors.initials: GA
authors.lastname: Cardineau
authors.affiliation: Tecnológico de Monterrey, Escuela Nacional de Medicina, Monterrey, Nuevo León, México.
authors.forename: Sean-Patrick
authors.initials: SP
authors.lastname: Scott
mesh_terms: D000368:Aged; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D053208:Kaplan-Meier Estimate; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology; D008297:Male; D008800:Mexico; D008875:Middle Aged; D056950:Period Circadian Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis
chemical_list: C512644:PER3 protein, human; D056950:Period Circadian Proteins
keywords: Circadian rhythm; PER3; biomarker; diffuse large B-cell lymphoma; single nucleotide polymorphism; survival