title: Adhering maternal platelets can contribute to the cytokine and chemokine cocktail released by human first trimester villous placenta.
abstract: Placental villous explant culture has been increasingly recognized as suitable model to study secretion of inflammatory and immune modulating factors by human placenta. Most of these factors likely derive from the syncytiotrophoblast, whereas extraplacental sources such as maternal peripheral blood cells are rarely considered. Due to their small size and absence of a nucleus, platelets adhering to perivillous fibrinoid of normal placenta are frequently ignored in routine immunohistochemistry. Here we demonstrate adhering maternal platelets on first trimester placental villi after explant culture and point out that platelet-derived factors must be considered when analyzing the inflammatory secretion profile of human placenta.
authors.affiliation: Institute of Cell Biology, Histology and Embryology, Medical University Graz, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Blaschitz
authors.affiliation: Institute of Cell Biology, Histology and Embryology, Medical University Graz, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Siwetz
authors.affiliation: Department of Blood Group Serology and Transfusion Medicine, Medical University Graz, Austria.
authors.forename: P
authors.initials: P
authors.lastname: Schlenke
authors.affiliation: Institute of Cell Biology, Histology and Embryology, Medical University Graz, Austria. Electronic address: martin.gauster@medunigraz.at.
authors.forename: M
authors.initials: M
authors.lastname: Gauster
mesh_terms: D001792:Blood Platelets / Q000737:chemistry*; D005260:Female; D006801:Humans; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D046509:Tissue Culture Techniques
keywords: Chemokines; Cytokines; Placental explant culture; Platelets