title: An Arabidopsis PWI and RRM motif-containing protein is critical for pre-mRNA splicing and ABA responses.
abstract: The phytohormone abscisic acid (ABA) is important for growth, development and stress responses in plants. Recent research has identified ABA receptors and signalling components that regulate seed germination and stomatal closure. However, proteins that regulate ABA signalling remain poorly understood. Here we use a forward-genetic screen to identify rbm25-1 and rbm25-2, two Arabidopsis mutants with increased sensitivity to growth inhibition by ABA. Using RNA-seq, we found that RBM25 controls the splicing of many pre-mRNAs. The protein phosphatase 2C HAB1, a critical component in ABA signalling, shows a dramatic defect in pre-mRNA splicing in rbm25 mutants. Ectopic expression of a HAB1 complementary DNA derived from wild-type mRNAs partially suppresses the rbm25-2 mutant phenotype. We suggest that RNA splicing is of particular importance for plant response to ABA and that the splicing factor RBM25 has a critical role in this response.
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Xiangqiang
authors.initials: X
authors.lastname: Zhan
authors.affiliation: Department of Plant Science and Landscape Architecture, University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA.
authors.forename: Bilian
authors.initials: B
authors.lastname: Qian
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Fengqiu
authors.initials: F
authors.lastname: Cao
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Wenwu
authors.initials: W
authors.lastname: Wu
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Plant Science and Landscape Architecture, University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA.
authors.forename: Qingmei
authors.initials: Q
authors.lastname: Guan
authors.affiliation: Department of Plant Science and Landscape Architecture, University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA.
authors.forename: Xianbin
authors.initials: X
authors.lastname: Gu
authors.affiliation: Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Plant Science and Landscape Architecture, University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA.
authors.forename: Temiloluwa A
authors.initials: TA
authors.lastname: Okusolubo
authors.affiliation: Department of Plant Science and Landscape Architecture, University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA.
authors.forename: Stephanie L
authors.initials: SL
authors.lastname: Dunn
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.|Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Zhu
authors.affiliation: Department of Plant Science and Landscape Architecture, University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D020816:Amino Acid Motifs; D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D007532:Isoleucine; D009154:Mutation; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism; D011392:Proline; D000067776:RNA Recognition Motif Proteins / Q000235:genetics*; D012326:RNA Splicing / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D014364:Tryptophan
chemical_list: D029681:Arabidopsis Proteins; D000067776:RNA Recognition Motif Proteins; D012333:RNA, Messenger; D007532:Isoleucine; D000040:Abscisic Acid; D014364:Tryptophan; D011392:Proline; C514757:HAB1 protein, Arabidopsis; D010749:Phosphoprotein Phosphatases