title: Geosmin induces genomic instability in the mammalian cell microplate-based comet assay.
abstract: Geosmin (GEO) (trans-1,10-dimethyl-trans-9-decalol) is a metabolite that renders earthy and musty taste and odor to water. Data of GEO genotoxicity on mammalian cells are scarce in the literature. Thus, the present study assessed the genotoxicity of GEO on Chinese hamster ovary (CHO) cells in the microplate-based comet assay. The percent of tail DNA (tail intensity (TI)), tail moment (TM), and tail length (TL) were used as parameters for DNA damage assessment. The results demonstrated that concentrations of GEO of 30 and 60 μg/mL were genotoxic to CHO cells after 4- and 24-h exposure periods, in all parameters evaluated, such as TI, TM, and TL. Additionally, GEO 15 μg/mL was genotoxic in the three parameters only in the 24-h exposure time. The same was observed for GEO 7.5 μg/mL, which induced significant DNA damage observed as TI in the 24-h treatment. The results present evidence that exposure to GEO may be associated with genomic instability in mammalian cells.
authors.affiliation: Genetic Toxicity Laboratory (TOXIGEN), Post-Graduation Program in Molecular and Cellular Biology Applied to Health, Lutheran University of Brazil (ULBRA), Prédio 22, 4° andar, sala 20, Avenida Farroupilha, 8001, 92425-900, Canoas, RS, Brazil.
authors.forename: Aline Flor
authors.initials: AF
authors.lastname: Silva
authors.affiliation: Genetic Toxicity Laboratory (TOXIGEN), Post-Graduation Program in Molecular and Cellular Biology Applied to Health, Lutheran University of Brazil (ULBRA), Prédio 22, 4° andar, sala 20, Avenida Farroupilha, 8001, 92425-900, Canoas, RS, Brazil.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Lehmann
authors.affiliation: Genetic Toxicity Laboratory (TOXIGEN), Post-Graduation Program in Molecular and Cellular Biology Applied to Health, Lutheran University of Brazil (ULBRA), Prédio 22, 4° andar, sala 20, Avenida Farroupilha, 8001, 92425-900, Canoas, RS, Brazil. radihl@gmail.com.
authors.forename: Rafael Rodrigues
authors.initials: RR
authors.lastname: Dihl
mesh_terms: D000818:Animals; D016466:CHO Cells; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D004249:DNA Damage; D042822:Genomic Instability / Q000187:drug effects*; D009284:Naphthols / Q000737:chemistry / Q000633:toxicity*; D009812:Odorants; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
chemical_list: D009284:Naphthols; D014874:Water Pollutants, Chemical; D004247:DNA; C001278:geosmin
keywords: CHO cells; Comet assay; Geosmin; Tail intensity; Tail length; Tail moment