title: Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β1 (TGF-β1) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED).
  
  
  METHODS
  SHED were co-cultured with ERM with/without TGF-β1. Then, SHED proliferation, morphological appearance, alkaline phosphatase (ALP) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated.
  
  
  RESULTS
  TGF-β1 enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β1. This activity was demonstrated by high ALP activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e. ALP, collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein).
  
  
  CONCLUSIONS
  ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β1.
  
  
  CONCLUSIONS
  The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.
authors.affiliation: Conservative Department, School of Dental Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia. ghorafi44@yahoo.com.
authors.forename: Manal
authors.initials: M
authors.lastname: Farea
authors.affiliation: Conservative Department, School of Dental Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
authors.forename: Adam
authors.initials: A
authors.lastname: Husein
authors.affiliation: Reconstructive Sciences Unit, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
authors.forename: Ahmad Sukari
authors.initials: AS
authors.lastname: Halim
authors.affiliation: Conservative Department, School of Dental Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
authors.forename: Zurairah
authors.initials: Z
authors.lastname: Berahim
authors.affiliation: School of Health Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
authors.forename: Asma Abdullah
authors.initials: AA
authors.lastname: Nurul
authors.affiliation: Department of Oral Biology, Kulliyah of Dentistry, IIUM Kuantan Campus, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200, Kuantan Pahang DM, Malaysia.
authors.forename: Khairani Idah
authors.initials: KI
authors.lastname: Mokhtar
authors.affiliation: Conservative Department, School of Dental Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
authors.forename: Kasmawati
authors.initials: K
authors.lastname: Mokhtar
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D018920:Coculture Techniques; D003739:Dental Cementum / Q000166:cytology*; D003746:Dental Enamel Proteins / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology*; D015870:Gene Expression; D006801:Humans; D010012:Osteogenesis / Q000502:physiology*; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology*; D014094:Tooth, Deciduous / Q000166:cytology*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
chemical_list: D003746:Dental Enamel Proteins; D053773:Transforming Growth Factor beta1
keywords: Cementogenic differentiation; Epithelial rests of Malassez cells; Osteogenic differentiation; Stem cells of human exfoliated deciduous teeth; Transforming growth factor-β1