title: MMP 9 Gene Promoter Polymorphism in Gastric Cancer.
abstract: Matrix metalloproteinase-9 (gelatinase B) plays a key role in cancer invasion and metastasis by degrading the extracellular matrix and basement membrane barriers. A cytosine (C) > thymidine (T) single nucleotide polymorphism (SNP) at position -1562 in the MMP-9 promoter is reported to influence the expression of the gene. Genotyping of MMP-9 -1562 C→T promoter polymorphism in 140 gastric cancer patients and 132 healthy control subjects was carried out in order to evaluate its association with progression and development of gastric cancer. The SNP was genotyped by tetra-primer amplification refractory mutation system-polymerase chain reaction followed by agarose gel electrophoresis. Statistical methods were adopted to test for the significance of the results. Risk factor profile of the patients revealed age above 50 years, smoking, alcoholism as the factors associated with the disease. The distribution of genotype frequencies in gastric cancer patients were 28.7 % of CC, 45.5 % of CT and 25.7 % of TT, whereas in control subjects 31.8 % of CC, 53.03 % of CT and 15.15 % of TT, respectively. The allelic frequencies were 51.51 % of C and 48.48 % of T in patient group and 58.33 % of C and 41.66 % of T in controls respectively. The present study shows the possible association of epidemiological risk factors with gastric cancer. There is an increased frequency of T allele in the disease compared to control subjects. However, there is no association of the MMP-9 -1562 C→T promoter polymorphism in the development of gastric cancer.
authors.affiliation: Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500 016 India.
authors.forename: D
authors.initials: D
authors.lastname: Krishnaveni
authors.affiliation: Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500 016 India.
authors.forename: Amar Chand
authors.initials: AC
authors.lastname: Bhayal
authors.affiliation: Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500 016 India.
authors.forename: K
authors.initials: K
authors.lastname: Sri Manjari
authors.affiliation: Department of Gastroenterology, Gandhi Hospital, Hyderabad, 500 003 India.
authors.forename: A
authors.initials: A
authors.lastname: Vidyasagar
authors.affiliation: Department of Gastroenterology, Gandhi Hospital, Hyderabad, 500 003 India.
authors.forename: M
authors.initials: M
authors.lastname: Uma Devi
authors.affiliation: Department of Gastroenterology, Gandhi Hospital, Hyderabad, 500 003 India.
authors.forename: M
authors.initials: M
authors.lastname: Ramanna
authors.affiliation: Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500 016 India.
authors.forename: A
authors.initials: A
authors.lastname: Jyothy
authors.affiliation: Department of Genetics, Osmania University, Hyderabad, 500 007 India.
authors.forename: Pratibha
authors.initials: P
authors.lastname: Nallari
authors.affiliation: Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500 016 India.
authors.forename: A
authors.initials: A
authors.lastname: Venkateshwari
keywords: Gastric cancer; Matrix metalloproteinase-9; Metastasis; Polymorphism