title: DENTAL PULP STEM CELLS AND HUMAN PERIAPICAL CYST MESENCHYMAL STEM CELLS IN BONE TISSUE REGENERATION: COMPARISON OF BASAL AND OSTEOGENIC DIFFERENTIATED GENE EXPRESSION OF A NEWLY DISCOVERED MESENCHYMAL STEM CELL LINEAGE.
abstract: Bone regeneration is an interesting field of biomedicine. The most recent studies are aimed to achieve a bone regeneration using mesenchymal stem cells (MSCs) taken from more accessible sites: oral and dental tissues have been widely investigated as a rich accessible source of MSCs. Dental Pulp Stem Cells (DPSCs) and human Periapical Cysts Mesenchymal Stem Cells (hPCy-MSCs) represent the new generation MSCs. The aim of this study is to compare the gene expression of these two innovative cell types to highlight the advantages of their use in bone regeneration. The harvesting, culturing and differentiating of cells isolated from dental pulp as well as from periapical cystic tissue were carried out as described in previously published reports. qRT-PCR analyses were performed on osteogenic genes in undifferentiated and osteogenic differentiated cells of DPSC and hPCy-MSC lineage. Real-time RT-PCR data suggested that both DPSCs and hPCy-MSCs cultured in osteogenic media are able to differentiate into osteoblast/odontoblast-like cells: however, some differences indicated that DPSCs seem to be directed more towards dentinogenesis, while hPCy-MSCs seem to be directed more towards osteogenesis.
authors.affiliation: Tecnologica Research Institute, Biomedical Section, Stem Cells Unit, Crotone, Italy; Calabrodental Clinic, Biomedicine Unit, Crotone, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Tatullo
authors.affiliation: Department of Life, Health and Environmental Sciences, School of Dentistry, University of LAquila, LAquila, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Falisi
authors.affiliation: Tecnologica Research Institute, Biomedical Section, Stem Cells Unit, Crotone, Italy; Calabrodental Clinic, Biomedicine Unit, Crotone, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Amantea
authors.affiliation: Department of Life, Health and Environmental Sciences, School of Dentistry, University of LAquila, LAquila, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Rastelli
authors.affiliation: Tecnologica Research Institute, Biomedical Section, Stem Cells Unit, Crotone, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Paduano
authors.affiliation: Tecnologica Research Institute, Biomedical Section, Stem Cells Unit, Crotone, Italy; Calabrodental Clinic, Biomedicine Unit, Crotone, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Marrelli
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D003782:Dental Pulp / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010012:Osteogenesis; D011842:Radicular Cyst / Q000378:metabolism*