title: Comparative analysis of rabbit hemorrhagic disease virus (RHDV) and new RHDV2 virus antigenicity, using specific virus-like particles.
abstract: In 2010 a new Lagovirus related to rabbit haemorrhagic disease virus (RHDV) emerged in France and has since rapidly spread throughout domestic and wild rabbit populations of several European countries. The new virus, termed RHDV2, exhibits distinctive genetic, antigenic and pathogenic features. Notably, RHDV2 kills rabbits previously vaccinated with RHDV vaccines. Here we report for the first time the generation and characterization of RHDV2-specific virus-like particles (VLPs). Our results further confirmed the differential antigenic properties exhibited by RHDV and RHDV2, highlighting the need of using RHDV2-specific diagnostic assays to monitor the spread of this new virus.
authors.affiliation: Centro de Investigación en Sanidad Animal (INIA-CISA), Valdeolmos, Madrid, Spain. barcena@inia.es.
authors.forename: Juan
authors.initials: J
authors.lastname: Bárcena
authors.affiliation: Centro de Investigación en Sanidad Animal (INIA-CISA), Valdeolmos, Madrid, Spain. beatriz.guerra@inia.es.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Guerra
authors.affiliation: Centro de Investigación en Sanidad Animal (INIA-CISA), Valdeolmos, Madrid, Spain. ivan_angulo@hotmail.com.
authors.forename: Iván
authors.initials: I
authors.lastname: Angulo
authors.affiliation: Villamagna SA, Finca "La Garganta", Villanueva de Córdoba, Córdoba, Spain. julsglezglez@gmail.com.
authors.forename: Julia
authors.initials: J
authors.lastname: González
authors.affiliation: Centro de Investigación en Sanidad Animal (INIA-CISA), Valdeolmos, Madrid, Spain. valcarcel.felix@inia.es.
authors.forename: Félix
authors.initials: F
authors.lastname: Valcárcel
authors.affiliation: Department of Structure of Macromolecules, Centro Nacional de Biotecnología/CSIC, Cantoblanco, Madrid, Spain. cperez@cnb.csic.es.
authors.forename: Carlos P
authors.initials: CP
authors.lastname: Mata
authors.affiliation: Department of Structure of Macromolecules, Centro Nacional de Biotecnología/CSIC, Cantoblanco, Madrid, Spain. jrcaston@cnb.csic.es.
authors.forename: José R
authors.initials: JR
authors.lastname: Castón
authors.affiliation: Centro de Investigación en Sanidad Animal (INIA-CISA), Valdeolmos, Madrid, Spain. blanco@inia.es.
authors.forename: Esther
authors.initials: E
authors.lastname: Blanco
authors.affiliation: Centro de Investigación en Sanidad Animal (INIA-CISA), Valdeolmos, Madrid, Spain. alejo@inia.es.
authors.forename: Alí
authors.initials: A
authors.lastname: Alejo
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D017250:Caliciviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017929:Hemorrhagic Disease Virus, Rabbit / Q000235:genetics / Q000276:immunology; D029321:Lagovirus / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D011817:Rabbits; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D000956:Antigens, Viral