title: Sublethal imidacloprid effects on honey bee flower choices when foraging.
abstract: Neonicotinoids, systemic neuro-active pesticides similar to nicotine, are widely used in agriculture and are being investigated for a role in honey bee colony losses. We examined one neonicotinoid pesticide, imidacloprid, for its effects on the foraging behavior of free-flying honey bees (Apis mellifera anatoliaca) visiting artificial blue and white flowers. Imidacloprid doses, ranging from 1/5 to 1/50 of the reported LD50, were fed to bees orally. The study consisted of three experimental parts performed sequentially without interruption. In Part 1, both flower colors contained a 4 μL 1 M sucrose solution reward. Part 2 offered bees 4 μL of 1.5 M sucrose solution in blue flowers and a 4 μL 0.5 M sucrose solution reward in white flowers. In Part 3 we reversed the sugar solution rewards, while keeping the flower color consistent. Each experiment began 30 min after administration of the pesticide. We recorded the percentage of experimental bees that returned to forage after treatment. We also recorded the visitation rate, number of flowers visited, and floral reward choices of the bees that foraged after treatment. The forager return rate declined linearly with increasing imidacloprid dose. The number of foraging trips by returning bees was also affected adversely. However, flower fidelity was not affected by imidacloprid dose. Foragers visited both blue and white flowers extensively in Part 1, and showed greater fidelity for the flower color offering the higher sugar solution reward in Parts 2 and 3. Although larger samples sizes are needed, our study suggests that imidacloprid may not affect the ability to select the higher nectar reward when rewards were reversed. We observed acute, mild effects on foraging by honey bees, so mild that storage of imidacloprid tainted-honey is very plausible and likely to be found in honey bee colonies.
authors.affiliation: Department of Plant Protection, Suleyman Demirel University, Isparta, Republic of Turkey.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Karahan
authors.affiliation: Beekeeping Development Application and Research Center, MKP MYO, Uludag University, Bursa, Republic of Turkey. icakmak@uludag.edu.tr.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Çakmak
authors.affiliation: Department of Biological and Allied Health Sciences, Bloomsburg University, Bloomsburg, PA, 17815-1301, USA.
authors.forename: John M
authors.initials: JM
authors.lastname: Hranitz
authors.affiliation: Department of Plant Protection, Suleyman Demirel University, Isparta, Republic of Turkey.
authors.forename: Ismail
authors.initials: I
authors.lastname: Karaca
authors.affiliation: Department of Biology, University of Tulsa, Tulsa, OK, 74104, USA.
authors.forename: Harrington
authors.initials: H
authors.lastname: Wells
mesh_terms: D000818:Animals; D001516:Bees / Q000187:drug effects* / Q000502:physiology*; D002755:Choice Behavior / Q000187:drug effects; D005247:Feeding Behavior / Q000187:drug effects; D035264:Flowers; D007093:Imidazoles / Q000009:adverse effects*; D007306:Insecticides / Q000009:adverse effects*; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000009:adverse effects*
chemical_list: D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; C082359:imidacloprid
keywords: Apis mellifera; Foraging behavior; Neonicotinoids