title: An Apta-Biosensor for Colon Cancer Diagnostics.
abstract: This paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are used in this study to demonstrate the functionality of aptasensor for colon cancer detection purposes. Flow cytometry, fluorescence microscopy and electrochemical cyclic voltammetry are used to verify the binding between the target cells and aptamer. The limit of detection (LOD) of this aptasensor is equal to seven cancer cells. Based on the experimental results, the proposed sensor can be employed for point-of-care cancer disease diagnostics.
authors.affiliation: Department of Nanobiotechnology, School of New Sciences and Technologies, University of Tehran, Tehran 14395-1561, Iran. m_ahmadzadeh@ut.ac.ir.|Research Center of New Technologies in Life Science Engineering, University of Tehran, Tehran 1417963891, Iran. m_ahmadzadeh@ut.ac.ir.
authors.forename: Mojgan Ahmadzadeh
authors.initials: MA
authors.lastname: Raji
authors.affiliation: Department of Biotechnology and Pharmacy Engineering, Faculty of Chemical Engineering, University of Tehran, Tehran 4563-11155, Iran. amoabediny@ut.ac.ir.|Research Center of New Technologies in Life Science Engineering, University of Tehran, Tehran 1417963891, Iran. amoabediny@ut.ac.ir.
authors.forename: Ghasem
authors.initials: G
authors.lastname: Amoabediny
authors.affiliation: Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran 1419963111, Iran. ptajik@ut.ac.ir.
authors.forename: Parviz
authors.initials: P
authors.lastname: Tajik
authors.affiliation: Department of Nanobiotechnology, School of New Sciences and Technologies, University of Tehran, Tehran 14395-1561, Iran. hosseini_m@ut.ac.ir.
authors.forename: Morteza
authors.initials: M
authors.lastname: Hosseini
authors.affiliation: Department of Electrical Engineering and Computer Science, York University, Toronto, ON M3J1P3, Canada. egz@cse.yorku.ca.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Ghafar-Zadeh
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000175:diagnosis*; D003198:Computer Simulation; D055664:Electrochemical Techniques; D004566:Electrodes; D005434:Flow Cytometry; D006801:Humans; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data
chemical_list: D052157:Aptamers, Nucleotide
keywords: aptamer; aptasensor; biosensor; colon cancer; cyclic voltammetry (CV); fluorescence labeling