title: WEREWOLF and ENHANCER of GLABRA3 are interdependent regulators of the spatial expression pattern of GLABRA2 in Arabidopsis.
abstract: In multicellular organisms, cell fates are specified through differential regulation of transcription. Epidermal cell fates in the Arabidopsis thaliana root are precisely specified by several transcription factors, with the GLABRA2 (GL2) homeodomain protein acting at the farthest downstream in this process. To better understand the regulation of GL2 expression, we ectopically expressed WEREWOLF (WER) and ENHANCER OF GLABRA3 (EGL3) in various tissues and examined GL2 expression. Here we show that WER expressed ubiquitously in the root induced GL2 expression only in the root epidermis, whereas co-expression of WER and EGL3 induced GL2 expression in the corresponding tissues. We also found that GL3 accumulated in the nucleus at the early meristematic region and EGL3 accumulated later in the nucleus of epidermal cells. We further found that ectopic expression of WER and EGL3 in ground tissues inhibited GL2 expression in the epidermis. Our results suggest that the co-expression of WER and EGL3 is sufficient for driving GL2 and CPC expression.
authors.affiliation: Department of Biology, Chosun University, Gwangju 501-759, Republic of Korea.
authors.forename: Sang-Kee
authors.initials: SK
authors.lastname: Song
authors.affiliation: Department of Biology, Long Island University, 1 University Plaza, Brooklyn, NY, USA.
authors.forename: Su-Hwan
authors.initials: SH
authors.lastname: Kwak
authors.affiliation: University College, Yonsei University, 85 Songdogwahak-ro, Yeonsu-gu, Incheon 21983, Republic of Korea.
authors.forename: Soo Chul
authors.initials: SC
authors.lastname: Chang
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University Avenue, Ann Arbor, MI, USA.
authors.forename: John
authors.initials: J
authors.lastname: Schiefelbein
authors.affiliation: Department of Systems Biology, Yonsei University, 50 Yonsei-ro, Seoul 03722, Republic of Korea. Electronic address: mmlee@yonsei.ac.kr.
authors.forename: Myeong Min
authors.initials: MM
authors.lastname: Lee
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018398:Homeodomain Proteins / Q000235:genetics*; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; C511989:CPC protein, Arabidopsis; D004268:DNA-Binding Proteins; C528950:EGL3 protein, Arabidopsis; C089991:GL2 protein, Arabidopsis; D018398:Homeodomain Proteins; D020598:Proto-Oncogene Proteins c-myb; C402111:WER protein, Arabidopsis
keywords: Arabidopsis thaliana; CAPRICE; EHANCER OF GLABRA3; GLABRA2; Root hair; WEREWOLF