title: Differentiation of stem cells into insulin-producing cells under the influence of nanostructural polyoxometalates.
abstract: Two polyoxometalates (POMs) with W were synthesized by a two-step, self-assembling method. They were used for stimulation of mesenchymal stem cell differentiation into insulin-producing cells. The nanocompounds (tris(vanadyl)-substituted tungsto-antimonate(III) anions [POM1] and tris-butyltin-21-tungsto-9-antimonate(III) anions [POM2]) were characterized by analytical techniques, including ultraviolet-visible, Fourier transform infrared, nuclear magnetic resonance spectroscopy, and transmission electron microscopy. We found that these polyoxotungstates, with 2-4 nm diameters, did not present toxic effects at the tested concentrations. In vitro, POM1 stimulated differentiation of a greater number of dithizone-positive cells (also organized in clusters) than the second nanocompound (POM2). Based on our in vitro studies, we have concluded that both the POMs tested had significant biological activity acting as active stimuli for differentiation of stem cells into insulin-producing cells.
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Medicine, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, România.|Department of Inorganic Chemistry, Faculty of Chemistry and Chemical Engineering, "Babeş-Bolyai" University, Cluj-Napoca, România.
authors.forename: Ştefana
authors.initials: Ş
authors.lastname: Bâlici
authors.affiliation: Department of Morphological Sciences, Faculty of Medicine, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, România.|Imogen Research Centre - Department of Pathology, Cluj-Napoca, România.|Radiotherapy, Tumor and Radiobiology Laboratory, The Oncology Institute "Prof. Dr. Ion Chiricuţă", Cluj-Napoca, România.
authors.forename: Sergiu
authors.initials: S
authors.lastname: Şuşman
authors.affiliation: Department of Physical-Chemistry, Faculty of Pharmacy, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, România.
authors.forename: Dan
authors.initials: D
authors.lastname: Rusu
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Medicine, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, România.
authors.forename: Gheorghe Zsolt
authors.initials: GZ
authors.lastname: Nicula
authors.affiliation: Radiotherapy, Tumor and Radiobiology Laboratory, The Oncology Institute "Prof. Dr. Ion Chiricuţă", Cluj-Napoca, România.
authors.forename: Olga
authors.initials: O
authors.lastname: Soriţău
authors.affiliation: Department of Inorganic Chemistry, Faculty of Chemistry and Chemical Engineering, "Babeş-Bolyai" University, Cluj-Napoca, România.
authors.forename: Mariana
authors.initials: M
authors.lastname: Rusu
authors.affiliation: Center for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, Little Rock, AR, USA.
authors.forename: Alexandru S
authors.initials: AS
authors.lastname: Biris
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Medicine, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, România.
authors.forename: Horea
authors.initials: H
authors.lastname: Matei
mesh_terms: D053687:Adult Stem Cells / Q000187:drug effects* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity; D046529:Microscopy, Electron, Transmission; D010641:Phenotype; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D017973:Tungsten Compounds / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D000099294:Polyoxometalates
chemical_list: D007328:Insulin; D017973:Tungsten Compounds; C102296:polyoxometalate I; D000099294:Polyoxometalates
keywords: butyltin; diabetes; insulin; polyoxometalates; stem cells; tungsten; vanadium