title: Biodiversity, Anti-Trypanosomal Activity Screening, and Metabolomic Profiling of Actinomycetes Isolated from Mediterranean Sponges.
abstract: Marine sponge-associated actinomycetes are considered as promising sources for the discovery of novel biologically active compounds. In the present study, a total of 64 actinomycetes were isolated from 12 different marine sponge species that had been collected offshore the islands of Milos and Crete, Greece, eastern Mediterranean. The isolates were affiliated to 23 genera representing 8 different suborders based on nearly full length 16S rRNA gene sequencing. Four putatively novel species belonging to genera Geodermatophilus, Microlunatus, Rhodococcus and Actinomycetospora were identified based on a 16S rRNA gene sequence similarity of < 98.5% to currently described strains. Eight actinomycete isolates showed bioactivities against Trypanosma brucei brucei TC221 with half maximal inhibitory concentration (IC50) values <20 μg/mL. Thirty four isolates from the Milos collection and 12 isolates from the Crete collection were subjected to metabolomic analysis using high resolution LC-MS and NMR for dereplication purposes. Two isolates belonging to the genera Streptomyces (SBT348) and Micromonospora (SBT687) were prioritized based on their distinct chemistry profiles as well as their anti-trypanosomal activities. These findings demonstrated the feasibility and efficacy of utilizing metabolomics tools to prioritize chemically unique strains from microorganism collections and further highlight sponges as rich source for novel and bioactive actinomycetes.
authors.affiliation: Dept. of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg, Würzburg, Germany.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.
authors.forename: Lynsey
authors.initials: L
authors.lastname: MacIntyre
authors.affiliation: Dept. of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg, Würzburg, Germany.
authors.forename: Usama Ramadan
authors.initials: UR
authors.lastname: Abdelmohsen
authors.affiliation: Dept. of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg, Würzburg, Germany.
authors.forename: Hannes
authors.initials: H
authors.lastname: Horn
authors.affiliation: Hellenic Centre for Marine Research, Institute of Marine Biology, Biotechnology and Aquaculture, Gournes Pediados, Heraklion, Crete, Greece.
authors.forename: Paraskevi N
authors.initials: PN
authors.lastname: Polymenakou
authors.affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.
authors.forename: RuAngelie
authors.initials: R
authors.lastname: Edrada-Ebel
authors.affiliation: Dept. of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg, Würzburg, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Hentschel
mesh_terms: D039903:Actinobacteria / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D000818:Animals; D000981:Antiprotozoal Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D044822:Biodiversity; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology; D055808:Drug Discovery; D008522:Mediterranean Sea; D055442:Metabolome; D055432:Metabolomics; D008969:Molecular Sequence Data; D021261:Parasitic Sensitivity Tests; D010802:Phylogeny; D011161:Porifera / Q000382:microbiology*; D014346:Trypanosoma brucei brucei / Q000187:drug effects / Q000254:growth & development
chemical_list: D000981:Antiprotozoal Agents; D001688:Biological Products