title: Circular Dichroism for the Analysis of Protein-DNA Interactions.
abstract: The aim of this chapter is to provide information on the practical aspects of circular dichroism (CD) and synchrotron radiation circular dichroism (SRCD) in protein-nucleic acids interaction solution studies. The chapter will describe the guidelines appropriate to designing experiments and conducting correct data interpretation, the use of both benchtop and synchrotron CD approaches is discussed and the advantages of SRCD outlined. Further information and a good general review of the field a can be found in Gray (Circular Dichroism of protein-nucleic acid interactions. In: Fasman GD (ed) Circular dichroism and the conformational analysis of biomolecules. Plenum Press, New York. pp 469-500, 1996).
authors.affiliation: Institute of Biomedical and Biomolecular Sciences, School of Biological Sciences, University of Portsmouth, King Henry 1st Building, Portsmouth, PO1 2DY, UK. garry.scarlett@port.ac.uk.
authors.forename: Garry
authors.initials: G
authors.lastname: Scarlett
authors.affiliation: Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, UK.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Siligardi
authors.affiliation: Institute of Biomedical and Biomolecular Sciences, School of Biological Sciences, University of Portsmouth, King Henry 1st Building, Portsmouth, PO1 2DY, UK.
authors.forename: Geoffrey G
authors.initials: GG
authors.lastname: Kneale
mesh_terms: D002942:Circular Dichroism / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D009690:Nucleic Acid Conformation; D017356:Synchrotrons
chemical_list: D004268:DNA-Binding Proteins; D004247:DNA
keywords: Circular dichroism; DNA–protein interactions; Synchrotron CD