title: Quercetin promotes neurite growth through enhancing intracellular cAMP level and GAP-43 expression.
abstract: The present study was designed to investigate the role of quercetin on neurite growth in N1E-115 cells and the underlying mechanisms. Quercetin was evaluated for its effects on cell numbers of neurites, neurite length, intracellular cAMP content, and Gap-43 expression in N1E-115 cells in vitro by use of microscopy, LANCE(tm) cAMP 384 kit, and Western blot analysis, respectively. Our results showed that quercetin could increase the neurite length in a concentration-dependent manner, but had no effect on the numbers of cells. Quercetin significantly increased the expression of cellular cAMP in a time- and concentration-dependent manner. The Gap-43 expression was up-regulated in a time-dependent manner. In conclusion, quercetin could promote neurite growth through increasing the intracellular cAMP level and Gap-43 expression.
authors.affiliation: National Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Chen
authors.affiliation: Department of Phytochemistry, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Yin
authors.affiliation: National Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. Electronic address: drugscreen@126.com.
authors.forename: Lu-Yong
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: National Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. Electronic address: hliao@cpu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Liao
mesh_terms: D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism*; D019922:GAP-43 Protein / Q000378:metabolism*; D009416:Nerve Regeneration; D016501:Neurites / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology*; D015398:Signal Transduction
chemical_list: D019922:GAP-43 Protein; D010936:Plant Extracts; D011794:Quercetin; D000242:Cyclic AMP
keywords: Gap-43; N1E-115; Quercetin; cAMP; neurite growth