title: Proteomics analysis of tissue samples from patients with squamous cell carcinoma of the penis and positive to human papillomavirus.
abstract: OBJECTIVE
  The aim of this study was to identify possible protein biomarkers and/or candidates for therapeutic targets in tissues of patients with SCCP, infected by HPV, applying one dimensional electrophoresis (1DE), followed by direct mass spectrometry (MS) analysis.
  
  
  METHODS
  Tissues from 10 HPV positive patients with SCCP and from 10 patients with HPV negative non-tumorous penile foreskins were analyzed applying 1D electrophoresis, followed by analysis with direct mass spectrometry (MS).
  
  
  RESULTS
  Sixty-three different proteins were identified in the first group and 50 in the second group. Recognition was possible for 28 proteins exclusively detected in Group 1 and 21 proteins presented only in Group 2.
  
  
  CONCLUSIONS
  Some proteins in the first group are directly involved in the development of other types of cancer, and therefore, suitable for analysis. Complement C3 protein is a strong candidate for evaluating SCCP patients.
authors.affiliation: Serviço de Urologia, Hospital Municipal Souza Aguiar, Rio de Janeiro, Rio de Janeiro,Brasil.|Serviço de Urologia, Hospital Mário Kröeff, Rio de Janeiro, Rio de Janeiro, Brasil.|Serviço de Hematologia, Instituto Nacional de Câncer - Laboratório de Genética Aplicada, Rio de Janeiro, Rio de Janeiro, Brasil.
authors.forename: Leandro
authors.initials: L
authors.lastname: Koifman
authors.affiliation: Serviço de Hematologia, Instituto Nacional de Câncer - Laboratório de Genética Aplicada, Rio de Janeiro, Rio de Janeiro, Brasil.|Programa de Pós-Graduação em Ciências Médicas (PGCM), Universidade Estadual do Rio de Janeiro, Rio de Janeiro, Brasil.
authors.forename: Paulo
authors.initials: P
authors.lastname: Ornellas
authors.affiliation: Serviço de Urologia, Hospital Mário Kröeff, Rio de Janeiro, Rio de Janeiro, Brasil.|Departmento de Urologia, Instituto Nacional de Câncer, Rio de Janeiro, Brasil.
authors.forename: Antonio Augusto
authors.initials: AA
authors.lastname: Ornellas
authors.affiliation: Serviço de Hematologia, Instituto Nacional de Câncer - Laboratório de Genética Aplicada, Rio de Janeiro, Rio de Janeiro, Brasil.|Instituto Nacional de Câncer - Programa de Carcinogênese Molecular, Coordenação Geral de Ensino e Pesquisa, Rio de Janeiro, Brasil.|Universidade Federal do Rio de Janeiro - Instituto de Bioquímica Médica, Unidade de Espectrometria de Massas e Proteômica, Instituto Nacional de Biologia Estrutural e Bioimagem (INBEB), Rio de Janeiro, Brasil.
authors.forename: Denise de Abreu
authors.initials: Dde A
authors.lastname: Pereira
authors.affiliation: Universidade Federal do Rio de Janeiro - Instituto de Bioquímica Médica, Unidade de Espectrometria de Massas e Proteômica, Instituto Nacional de Biologia Estrutural e Bioimagem (INBEB), Rio de Janeiro, Brasil.
authors.forename: Benedeta Russolina
authors.initials: BR
authors.lastname: Zingali
authors.affiliation: Universidade Federal Fluminense - Laboratório de Diagnóstico Virológico, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Rio de Janeiro, Brasil.
authors.forename: Silvia Maria Baeta
authors.initials: SM
authors.lastname: Cavalcanti
authors.affiliation: Universidade Federal Fluminense - Laboratório de Diagnóstico Virológico, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Rio de Janeiro, Brasil.
authors.forename: Larissa Alves
authors.initials: LA
authors.lastname: Afonso
authors.affiliation: Serviço de Hematologia, Instituto Nacional de Câncer - Laboratório de Genética Aplicada, Rio de Janeiro, Rio de Janeiro, Brasil.|Universidade Federal do Rio de Janeiro - Instituto de Bioquímica Médica, Unidade de Espectrometria de Massas e Proteômica, Instituto Nacional de Biologia Estrutural e Bioimagem (INBEB), Rio de Janeiro, Brasil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Sandim
authors.affiliation: Serviço de Hematologia, Instituto Nacional de Câncer - Laboratório de Genética Aplicada, Rio de Janeiro, Rio de Janeiro, Brasil.
authors.forename: Gilda
authors.initials: G
authors.lastname: Alves
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000473:pathology / Q000821:virology; D003176:Complement C3 / Q000032:analysis; D030562:Databases, Protein; D004586:Electrophoresis; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000302:isolation & purification; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis*; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications*; D010412:Penile Neoplasms / Q000737:chemistry* / Q000473:pathology / Q000821:virology; D040901:Proteomics
chemical_list: D014408:Biomarkers, Tumor; D003176:Complement C3; D009363:Neoplasm Proteins