title: Phenotypic analysis of T cells infiltrating colon cancers: Correlations with oncogenetic status.
abstract: Colorectal cancers (CRC) develop in the face of an important immune system associated with the intestinal mucosal tissue. The immune response against the tumor has been proposed to affect the prognosis of patients undergoing treatment for CRC. In this study T cells infiltrating the tumor were compared with T cells populating the unaffected neighboring mucosal tissue and cells from the peripheral blood. We observed that T cells from the tumor harbor an activated phenotype, with engagement of the NKG2D pathway in CD8 T cells. We show that mucosal and tumor-infiltrating T cells are enriched in NKG2D CD4 T cells, which exhibit cytotoxic functions. Finally, T cell populations in the tumor were modified according to its oncogenetic status, with higher percentages of CD8 T cells isolated from patients with microsatellite instable tumor status.
authors.affiliation: INSERM U1160; Institut Universitaire d'Hématologie; Hôpital Saint-Louis ; Paris, France ; Univ Paris Diderot; Sorbonne Paris Cité ; Paris, France ; Surgery Department; Hôopital Saint-Louis; AP-HP ; Paris, France.
authors.forename: Mircea
authors.initials: M
authors.lastname: Chirica
authors.affiliation: INSERM U1160; Institut Universitaire d'Hématologie; Hôpital Saint-Louis ; Paris, France ; Univ Paris Diderot; Sorbonne Paris Cité ; Paris, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Le Bourhis
authors.affiliation: Univ Paris Diderot; Sorbonne Paris Cité ; Paris, France ; Department of Biochemistry; Molecular Oncology Unit; Hôpital Saint-Louis; AP-HP ; Paris, France ; CNRS UMR7212/INSERMU944; Hôpital Saint-Louis ; Paris, France.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Lehmann-Che
authors.affiliation: INSERM U1160; Institut Universitaire d'Hématologie; Hôpital Saint-Louis ; Paris, France ; Univ Paris Diderot; Sorbonne Paris Cité ; Paris, France.
authors.forename: Victor
authors.initials: V
authors.lastname: Chardiny
authors.affiliation: Univ Paris Diderot; Sorbonne Paris Cité ; Paris, France ; Department of Pathology; Hôpital Saint-Louis; AP-HP ; Paris, France.
authors.forename: Fatiha
authors.initials: F
authors.lastname: Bouhidel
authors.affiliation: INSERM U1160; Institut Universitaire d'Hématologie; Hôpital Saint-Louis ; Paris, France ; Univ Paris Diderot; Sorbonne Paris Cité ; Paris, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Foulboeuf
authors.affiliation: Univ Paris Diderot; Sorbonne Paris Cité ; Paris, France ; Gastroenterology Department; Hôpital Saint-Louis, AP-HP Paris, France.
authors.forename: Jean Marc
authors.initials: JM
authors.lastname: Gornet
authors.affiliation: Univ Paris Diderot; Sorbonne Paris Cité ; Paris, France ; Gastroenterology Department; Hôpital Saint-Louis, AP-HP Paris, France.
authors.forename: Nelson
authors.initials: N
authors.lastname: Lourenco
authors.affiliation: INSERM U1160; Institut Universitaire d'Hématologie; Hôpital Saint-Louis ; Paris, France ; Univ Paris Diderot; Sorbonne Paris Cité ; Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Dulphy
authors.affiliation: INSERM U1160; Institut Universitaire d'Hématologie; Hôpital Saint-Louis ; Paris, France ; Univ Paris Diderot; Sorbonne Paris Cité ; Paris, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Toubert
authors.affiliation: INSERM U1160; Institut Universitaire d'Hématologie; Hôpital Saint-Louis ; Paris, France ; Univ Paris Diderot; Sorbonne Paris Cité ; Paris, France ; Gastroenterology Department; Hôpital Saint-Louis, AP-HP Paris, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Allez
keywords: Colon cancer; NKG2D; T cells; microsatellite instability