title: Fitness Studies of Azole-Resistant Strains of Aspergillus fumigatus.
abstract: Isogenic bar-coded strains of Aspergillus fumigatus carrying the G54W or M220K mutation in Cyp51A were constructed. In vitro, the growth and conidiation capacities of the mutants were similar to those of the parental strain. Competition studies in the absence of azoles showed that there was no adverse fitness cost for the azole-resistant A. fumigatus strains in vitro or in vivo compared to the parental strain.
authors.affiliation: Unité des Aspergillus, Institut Pasteur, Paris, France.
authors.forename: Isabel
authors.initials: I
authors.lastname: Valsecchi
authors.affiliation: Mycology Reference Laboratory, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Emilia
authors.initials: E
authors.lastname: Mellado
authors.affiliation: Unité des Aspergillus, Institut Pasteur, Paris, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Beau
authors.affiliation: Unité des Aspergillus, Institut Pasteur, Paris, France.
authors.forename: Shriya
authors.initials: S
authors.lastname: Raj
authors.affiliation: Unité des Aspergillus, Institut Pasteur, Paris, France jean-paul.latge@pasteur.fr.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Latgé
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D001228:Aspergillosis / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D001393:Azoles / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D025141:Drug Resistance, Fungal / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D056084:Genetic Fitness; D008168:Lung / Q000187:drug effects / Q000382:microbiology; D008297:Male; D051379:Mice; D009154:Mutation; D013172:Spores, Fungal / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000472:pathogenicity; D014774:Virulence
chemical_list: D000935:Antifungal Agents; D001393:Azoles; D005656:Fungal Proteins; D003577:Cytochrome P-450 Enzyme System; C465526:cytochrome P-450 CYP51A, Aspergillus