title: Effect of hrWnt7a on Human Retinal Pigment Epithelial Cells In Vitro.
abstract: Human recombinant protein Wnt7a (hrWnt7a) inhibits cell proliferative activity and triggers cell polarization. Although cell polarization process was maintained only over a short time, probably via microenvironmental stimuli, hrWnt7a is involved in the transformation of the retinal pigment epithelium. Analysis of Wnt signaling pathway and its regulation will help to understand the processes in retinal pigment epithelial cells under pathological conditions, which can be useful in developing new generation drugs.
authors.affiliation: N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. avkuzn@list.ru.
authors.forename: A V
authors.initials: AV
authors.lastname: Kuznetsova
authors.affiliation: N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: A M
authors.initials: AM
authors.lastname: Kurinov
authors.affiliation: Helmholtz Moscow Research Institute of Eye Diseases, Moscow, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Chentsova
authors.affiliation: Helmholtz Moscow Research Institute of Eye Diseases, Moscow, Russia.
authors.forename: P V
authors.initials: PV
authors.lastname: Makarov
authors.affiliation: N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.|V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Aleksandrova
mesh_terms: D002465:Cell Movement; D016764:Cell Polarity; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000502:physiology; D005260:Female; D006801:Humans; D008875:Middle Aged; D055213:Retinal Pigment Epithelium / Q000502:physiology*; D051153:Wnt Proteins / Q000502:physiology*
chemical_list: C092778:WNT7A protein, human; D051153:Wnt Proteins
keywords: MTT test; cell culture; human retinal pigment epithelium; immunohistochemistry; recombinant human protein Wnt7a