title: Comparative proteomics of Shigella flexneri 2a strain 301 using a rabbit ileal loop model reveals key proteins for bacterial adaptation in host niches.
abstract: OBJECTIVE
  Many studies focusing on changes in the host following Shigella spp invasion have been reported in recent years. However, the key factors required for the adaptation of these pathogens to host niches have usually been neglected.
  
  
  METHODS
  In this study, a comparative proteomic analysis was performed to examine changes in the protein expression profile of Shigella flexneri within the host using a rabbit ileal loop model to reveal proteins that are associated with pathogenic adaptation.
  
  
  RESULTS
  The protein expression profiles of bacteria isolated from the ileum and colon were very similar, although they differed slightly from that of bacteria isolated from the cecum. When compared with the sample in vitro, the expressions of seven proteins were found to be upshifted in vivo (OmpA, YgiW, MglB, YfiD, MetK, TktA, and AhpF), while two proteins were down-regulated (ElaB and GlnH).
  
  
  CONCLUSIONS
  The abundance of nine proteins changed in vivo, suggesting that these proteins may contribute to adaptation to the intestinal lumen.
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Xingming
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Lilan
authors.initials: L
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Xiankai
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Erling
authors.initials: E
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Dongshu
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China. Electronic address: jewly54@126.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China. Electronic address: wanghl@nic.bmi.ac.cn.
authors.forename: Hengliang
authors.initials: H
authors.lastname: Wang
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002432:Cecum / Q000382:microbiology; D003106:Colon / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D007082:Ileum / Q000382:microbiology*; D040901:Proteomics; D011817:Rabbits; D012763:Shigella flexneri / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins
keywords: Comparative proteomics; Mass spectrometry; Rabbit ileal loop model; Shigella flexneri; Two-dimensional gel electrophoresis