title: Biodegradation of cypermethrin by immobilized cells of Micrococcus sp. strain CPN 1.
abstract: Pyrethroid pesticide cypermethrin is a environmental pollutant because of its widespread use, toxicity and persistence. Biodegradation of such chemicals by microorganisms may provide an cost-effective method for their detoxification. We have investigated the degradation of cypermethrin by immobilized cells of Micrococcus sp. strain CPN 1 in various matrices such as, polyurethane foam (PUF), polyacrylamide, sodium alginate and agar. The optimum temperature and pH for the degradation of cypermethrin by immobilized cells of Micrococcus sp. were found to be 30 °C and 7.0, respectively. The rate of degradation of 10 and 20 mM of cypermethrin by freely suspended cells were compared with that of immobilized cells in batches and semi-continuous with shaken cultures. PUF-immobilized cells showed higher degradation of cypermethrin (10 mM and 20 mM) than freely suspended cells and cells immobilized in other matrices. The PUF-immobilized cells of Micrococcus sp. strain CPN 1 were retain their degradation capacity. Thus, they can be reused for more than 32 cycles, without losing their degradation capacity. Hence, the PUF-immobilized cells of Micrococcus sp. could potentially be used in the bioremediation of cypermethrin contaminated water.
authors.affiliation: Department of Biochemistry, Karnatak University, Karnataka, India .
authors.forename: Preeti N
authors.initials: PN
authors.lastname: Tallur
authors.affiliation: Department of Biochemistry, Karnatak University, Karnataka, India .
authors.forename: Sikandar I
authors.initials: SI
authors.lastname: Mulla
authors.affiliation: Department of Biochemistry, Karnatak University, Karnataka, India .
authors.forename: Veena B
authors.initials: VB
authors.lastname: Megadi
authors.affiliation: Department of Biochemistry, Karnatak University, Karnataka, India .
authors.forename: Manjunatha P
authors.initials: MP
authors.lastname: Talwar
authors.affiliation: Department of Biochemistry, Karnatak University, Karnataka, India .
authors.forename: Harichandra Z
authors.initials: HZ
authors.lastname: Ninnekar
mesh_terms: D000464:Alginates; D001673:Biodegradation, Environmental; D018914:Cells, Immobilized / Q000378:metabolism*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007306:Insecticides / Q000378:metabolism*; D008837:Micrococcus / Q000145:classification / Q000378:metabolism*; D011140:Polyurethanes; D011722:Pyrethrins / Q000378:metabolism*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D007306:Insecticides; D011140:Polyurethanes; D011722:Pyrethrins; C017160:cypermethrin; D020723:Glucuronic Acid; C028279:polyurethane foam
keywords: Micrococcus sp. strain CPN 1; cypermethrin; degradation; immobilization; polyurethane foam