title: Effect of age on the immune system and pathology of mice with chronic graft-versus-host disease lupus nephritis.
abstract: We investigated the effect of age on the expression of immune molecules [ANA, C4, double stranded DNA (dsDNA), CD16/32, CD19, CD3, and CD64], urine protein, and pathology in mice with chronic graft-versus-host disease (cGVHD) lupus nephritis (LN), and their relationship with reactivity index score. Mouse models of cGVHD LN were established, and mice were randomly divided into four aged-based groups of nine mice each. Serum levels of ANA, C4, and dsDNA were determined, the urine protein levels were assessed, and expression levels of CD16/32, CD19, CD3, and CD64 were measured. Expression levels of CD16/32+CD19(T1), CD16/CD32+CD3(T2), and CD64+CD3 or CD19(T3) were defined in the thymus, in bone marrow they were defined as CD16/32+CD19(B1), CD16/32+CD3(B2), CD64+CD3 or CD19(B3), and in spleen they were defined as CD16/32+CD19(P1), CD16/32+CD3(P2), CD64+CD3 or CD19(P3), respectively. There were significant differences in the levels of dsDNA and urine protein among the four groups (P < 0.05), which were negatively correlated with age. B1, B2, S1, and S2 were significantly different among the four groups (P < 0.05), with a positive correlation with age for B1 and B2. There was no correlation of expression of ANA, C4, dsDNA, T1-T3, B1-B3, S2-S3 with reactivity index score; S1 was the exception (r = -0.440, P = 0.011). Age influenced levels of dsDNA and urine protein in the mouse cGVHD model of LN. S1 was associated with reactivity index score and might also affect pathological changes.
authors.affiliation: The Immunology and Rheumatology Department, Affiliated Hospital of Weifang Medical University, Weifang, Shandong Province, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Ma
authors.affiliation: The Immunology and Rheumatology Department, Affiliated Hospital of Weifang Medical University, Weifang, Shandong Province, China mxfwjp188@163.com.
authors.forename: F L
authors.initials: FL
authors.lastname: Xu
authors.affiliation: The Immunology and Rheumatology Department, Affiliated Hospital of Weifang Medical University, Weifang, Shandong Province, China.
authors.forename: L F
authors.initials: LF
authors.lastname: Gao
authors.affiliation: The Immunology and Rheumatology Department, Affiliated Hospital of Weifang Medical University, Weifang, Shandong Province, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Wang
authors.affiliation: The Immunology and Rheumatology Department, Affiliated Hospital of Weifang Medical University, Weifang, Shandong Province, China.
authors.forename: Z B
authors.initials: ZB
authors.lastname: Pan
mesh_terms: D000367:Age Factors; D000818:Animals; D001323:Autoantibodies / Q000097:blood; D002908:Chronic Disease; D006086:Graft vs Host Disease / Q000097:blood / Q000276:immunology* / Q000473:pathology*; D007107:Immune System; D008181:Lupus Nephritis / Q000097:blood / Q000276:immunology* / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017452:Receptors, IgG / Q000276:immunology
chemical_list: D001323:Autoantibodies; D017452:Receptors, IgG