title: Simultaneous Detection and Evaluation of Four Subsets of CD4+ T Lymphocyte in Lesions and Peripheral Blood in Endometriosis.
abstract: OBJECTIVE
  The proportion of CD4+ T lymphocytes, Th1, Th2, Th17, and regulatory T cells in endometriosis lesions and peripheral blood are not known.
  
  
  METHODS
  Lymphocytes were isolated from endometriosis lesions (n = 10) and endometrium (n = 10). Lymphocytes in peripheral blood were isolated from patients with and without endometriosis (n = 10, 10). The CD4+ T-lymphocyte profile was analyzed by flow cytometry.
  
  
  RESULTS
  The proportion of Th1 lymphocytes was significantly lower in endometriosis tissue (59.64 ± 9.2%) in comparison with endometrial tissue (79.07 ± 8.97%), whereas the Th17 lymphocyte fraction was significantly higher in endometriosis tissue (6.66 ± 2.53%) in comparison with endometrial tissue (2.27 ± 1.51%). Analysis of peripheral blood indicated that the Th1 proportion was significantly higher in women with endometriosis (10.25 ± 2.82%) in comparison with controls (6.96 ± 4.13%).
  
  
  CONCLUSIONS
  The CD4+ T-lymphocyte profile in lesions and peripheral blood is altered in women with endometriosis. These findings may help better understanding of T-lymphocyte involvement in the pathophysiology of endometriosis.
authors.affiliation: Obstetrics and Gynecology, The University of Tokyo, Tokyo, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Takamura
authors.affiliation: Obstetrics and Gynecology, The University of Tokyo, Tokyo, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Koga
authors.affiliation: Obstetrics and Gynecology, The University of Tokyo, Tokyo, Japan.
authors.forename: Gentaro
authors.initials: G
authors.lastname: Izumi
authors.affiliation: Obstetrics and Gynecology, The University of Tokyo, Tokyo, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hirata
authors.affiliation: Obstetrics and Gynecology, The University of Tokyo, Tokyo, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Harada
authors.affiliation: Obstetrics and Gynecology, The University of Tokyo, Tokyo, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hirota
authors.affiliation: Obstetrics and Gynecology, The University of Tokyo, Tokyo, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Hiraike
authors.affiliation: Obstetrics and Gynecology, The University of Tokyo, Tokyo, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Fujii
authors.affiliation: Obstetrics and Gynecology, The University of Tokyo, Tokyo, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Osuga
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D016207:Cytokines / Q000378:metabolism; D004715:Endometriosis / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D016207:Cytokines
keywords: Endometrium; Th1; Th17; Th2; proportion; regulatory T cells