title: Biomimetic Self-Templated Hierarchical Structures of Collagen-Like Peptide Amphiphiles.
abstract: Developing hierarchically structured biomaterials with tunable chemical and physical properties like those found in nature is critically important to regenerative medicine and studies on tissue morphogenesis. Despite advances in materials synthesis and assembly processes, our ability to control hierarchical assembly using fibrillar biomolecules remains limited. Here, we developed a bioinspired approach to create collagen-like materials through directed evolutionary screening and directed self-assembly. We first synthesized peptide amphiphiles by coupling phage display-identified collagen-like peptides to long-chain fatty acids. We then assembled the amphiphiles into diverse, hierarchically organized, nanofibrous structures using directed self-assembly based on liquid crystal flow and its controlled deposition. The resulting structures sustained and directed the growth of bone cells and hydroxyapatite biominerals. We believe these self-assembling collagen-like amphiphiles could prove useful in the structural design of tissue regenerating materials.
authors.affiliation: Department of Bioengineering, University of California , Berkeley, California 94720, United States.|Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Hyo-Eon
authors.initials: HE
authors.lastname: Jin
authors.affiliation: Department of Genetic Engineering, College of Biotechnology & Bioengineering, Sungkyunkwan University , Suwon, Gyeonggi-do 440-746, Korea.
authors.forename: Jaein
authors.initials: J
authors.lastname: Jang
authors.affiliation: Department of Genetic Engineering, College of Biotechnology & Bioengineering, Sungkyunkwan University , Suwon, Gyeonggi-do 440-746, Korea.
authors.forename: Jinhyo
authors.initials: J
authors.lastname: Chung
authors.affiliation: Department of Bioengineering, University of California , Berkeley, California 94720, United States.
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Bioengineering, University of California , Berkeley, California 94720, United States.
authors.forename: Eddie
authors.initials: E
authors.lastname: Wang
authors.affiliation: Department of Bioengineering, University of California , Berkeley, California 94720, United States.|Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Seung-Wuk
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Department of Bioengineering, University of California , Berkeley, California 94720, United States.|Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.|Department of Genetic Engineering, College of Biotechnology & Bioengineering, Sungkyunkwan University , Suwon, Gyeonggi-do 440-746, Korea.
authors.forename: Woo-Jae
authors.initials: WJ
authors.lastname: Chung
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D032701:Biomimetics; D003094:Collagen / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D010455:Peptides; D003094:Collagen
keywords: biomimetic hierarchical structure; collagen-like peptide amphiphile; liquid crystalline assembly; nanofiber; self-templating; tissue regeneration