title: Marker-assisted selection in breeding silkworm strains with high tolerance to fluoride, scaleless wings, and high silk production.
abstract: In the silkworm (Bombyx mori), tolerance to fluoride and scaleless wings are controlled by the dominant gene Dtf (dominant tolerance to fluoride) and recessive gene nlw (no Lepidoptera wings), respectively, and these genes have been mapped by using simple sequence repeat and sequence tag site markers. Marker-assisted evaluation and selection of silkworms with fluoride tolerance and scaleless wings were used for predicting fluoride resistance and scaleless wings in backcrossed animals. A silkworm strain was bred using this method, and its economic characteristics were found to be similar to those of commercial silkworms. These methods will therefore be useful for silkworm breeding programs and in screening for two or more characteristics of interest for segregating populations.
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, China.
authors.forename: M W
authors.initials: MW
authors.lastname: Li
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, China.
authors.forename: H J
authors.initials: HJ
authors.lastname: Yu
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Yi
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: F Y
authors.initials: FY
authors.lastname: Dai
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, China cxhou587@163.com.
authors.forename: C X
authors.initials: CX
authors.lastname: Hou
mesh_terms: D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000033:anatomy & histology / Q000187:drug effects / Q000235:genetics*; D001947:Breeding; D004361:Drug Tolerance / Q000235:genetics; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D017344:Genes, Insect; D008040:Genetic Linkage; D005819:Genetic Markers; D006720:Homozygote; D007178:Inbreeding; D008297:Male; D018895:Microsatellite Repeats; D047011:Silk; D012969:Sodium Fluoride / Q000633:toxicity*; D014921:Wings, Animal / Q000033:anatomy & histology
chemical_list: D004785:Environmental Pollutants; D005819:Genetic Markers; D047011:Silk; D012969:Sodium Fluoride