title: Salt stress-induced modulations in the shoot proteome of Brassica juncea genotypes.
abstract: Indian mustard [Brassica juncea (L.) Czern and Coss] is cultivated mainly in the northwestern agroclimatic region of India and suffers huge losses in productivity due to salinization. In an effort to figure out adaptation strategies of Indian mustard to salt stress, we conducted a comparative proteome analysis of shoots of its two genotypes, with contrasting sensitivity to salt stress. Differential expression of 21 proteins was observed during the two-dimensional electrophoresis (2DE). The identified salt-stress-responsive proteins were associated with different functional processes including osmoregulation, photosynthesis, carbohydrate metabolism, ion homeostasis, protein synthesis and stabilization, energy metabolism, and antioxidant defense system. Salt-tolerant genotype (CS-52) showed a relatively higher expression of proteins involved in turgor regulation, stabilization of photosystems and proteins, and salt compartmentalization, as compared to salt-sensitive genotype (Pusa Varuna). Our results suggest that modulating the expression of salt-responsive proteins can pave the way for developing salt tolerance in the Indian mustard plants.
authors.affiliation: Molecular Ecology Laboratory, Department of Botany, Jamia Hamdard, New Delhi, 110062, India.
authors.forename: Peerzada Yasir
authors.initials: PY
authors.lastname: Yousuf
authors.affiliation: Molecular Ecology Laboratory, Department of Botany, Jamia Hamdard, New Delhi, 110062, India.
authors.forename: Altaf
authors.initials: A
authors.lastname: Ahmad
authors.affiliation: Molecular Ecology Laboratory, Department of Botany, Jamia Hamdard, New Delhi, 110062, India.
authors.forename: Arshid Hussain
authors.initials: AH
authors.lastname: Ganie
authors.affiliation: Molecular Ecology Laboratory, Department of Botany, Jamia Hamdard, New Delhi, 110062, India. iqbalg5@yahoo.co.in.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Iqbal
mesh_terms: D018506:Gene Expression Regulation, Plant; D005838:Genotype; D007194:India; D009149:Mustard Plant / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010788:Photosynthesis; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020543:Proteome / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D055049:Salt Tolerance; D012965:Sodium Chloride / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D020543:Proteome; D012965:Sodium Chloride
keywords: Gel electrophoresis; Ion homeostasis; Protein expression; Salinity stress