title: K88 Fimbrial Adhesin Targeting of Microspheres Containing Gentamicin Made with Albumin Glycated with Lactose.
abstract: The formulation and characterization of gentamicin-loaded microspheres as a delivery system targeting enterotoxigenic Escherichia coli K88 (E. coli K88) was investigated. Glycated albumin with lactose (BSA-glucose-β (4-1) galactose) was used as the microsphere matrix (MS-Lac) and gentamicin included as the transported antibiotic. The proposed target strategy was that exposed galactoses of MS-Lac could be specifically recognized by E. coli K88 adhesins, and the delivery of gentamicin would inhibit bacterial growth. Lactosylated microspheres (MS-Lac1, MS-Lac2 and MS-Lac3) were obtained using a water-in-oil emulsion, containing gentamicin, followed by crosslinking with different concentrations of glutaraldehyde. Electron microscopy displayed spherical particles with a mean size of 10-17 µm. In vitro release of gentamicin from MS-Lac was best fitted to a first order model, and the antibacterial activity of encapsulated and free gentamicin was comparable. MS-Lac treatments were recognized by plant galactose-specific lectins from Ricinus communis and Sophora japonica and by E. coli K88 adhesins. Results indicate MS-Lac1, produced with 4.2 mg/mL of crosslinker, as the best treatment and that lactosylated microsphere are promising platforms to obtain an active, targeted system against E. coli K88 infections.
authors.affiliation: Departamento de Investigacion en Fisica, Universidad de Sonora, Hermosillo Sonora 83000, Mexico. andreisarabia@gmail.com.|Laboratorio de Bioquimica de Proteinas y Glicanos, Coordinacion de Ciencia de los Alimentos, Centro de Investigacion en Alimentacion y Desarrollo A.C., Hermosillo Sonora 83304, Mexico. andreisarabia@gmail.com.
authors.forename: Andre-I
authors.initials: AI
authors.lastname: Sarabia-Sainz
authors.affiliation: Laboratorio de Bioquimica de Proteinas y Glicanos, Coordinacion de Ciencia de los Alimentos, Centro de Investigacion en Alimentacion y Desarrollo A.C., Hermosillo Sonora 83304, Mexico. hsarabiasainz@gmail.com.|Departamento de Investigacion y Posgrado en Alimentos, Universidad de Sonora, Hermosillo Sonora 83000, Mexico. hsarabiasainz@gmail.com.
authors.forename: Hector Manuel
authors.initials: HM
authors.lastname: Sarabia-Sainz
authors.affiliation: Laboratorio de Bioquimica de Proteinas y Glicanos, Coordinacion de Ciencia de los Alimentos, Centro de Investigacion en Alimentacion y Desarrollo A.C., Hermosillo Sonora 83304, Mexico. gramos@ciad.mx.
authors.forename: Gabriela Ramos-Clamont
authors.initials: GR
authors.lastname: Montfort
authors.affiliation: Laboratorio de Bioquimica de Proteinas y Glicanos, Coordinacion de Ciencia de los Alimentos, Centro de Investigacion en Alimentacion y Desarrollo A.C., Hermosillo Sonora 83304, Mexico. vmata@ciad.mx.
authors.forename: Veronica
authors.initials: V
authors.lastname: Mata-Haro
authors.affiliation: Laboratorio de Bioquimica de Proteinas y Glicanos, Coordinacion de Ciencia de los Alimentos, Centro de Investigacion en Alimentacion y Desarrollo A.C., Hermosillo Sonora 83304, Mexico. gupa@ciad.mx.
authors.forename: Ana María
authors.initials: AM
authors.lastname: Guzman-Partida
authors.affiliation: Department of Chemical and Environmental Engineering, the University of Arizona, Tucson, AZ 85721, USA. guzmanr@email.arizona.edu.
authors.forename: Roberto
authors.initials: R
authors.lastname: Guzman
authors.affiliation: Department of Chemical and Environmental Engineering, the University of Arizona, Tucson, AZ 85721, USA. mars4ward@gmail.com.
authors.forename: Mariano
authors.initials: M
authors.lastname: Garcia-Soto
authors.affiliation: Laboratorio de Bioquimica de Proteinas y Glicanos, Coordinacion de Ciencia de los Alimentos, Centro de Investigacion en Alimentacion y Desarrollo A.C., Hermosillo Sonora 83304, Mexico. lvazquez@ciad.mx.
authors.forename: Luz
authors.initials: L
authors.lastname: Vazquez-Moreno
mesh_terms: D000418:Albumins / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D000942:Antigens, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism*; D036781:Fimbriae Proteins / Q000378:metabolism*; D005839:Gentamicins / Q000008:administration & dosage* / Q000494:pharmacology; D007785:Lactose / Q000737:chemistry; D008863:Microspheres; D037121:Plant Lectins / Q000378:metabolism; D011485:Protein Binding
chemical_list: D000418:Albumins; D000900:Anti-Bacterial Agents; D000942:Antigens, Bacterial; D029968:Escherichia coli Proteins; D005839:Gentamicins; C026888:K88 antigen, E coli; D037121:Plant Lectins; D036781:Fimbriae Proteins; D007785:Lactose
keywords: gentamicin; lactosylated microspheres; targeted active drug