title: Epigenetic silencing of a long non-coding RNA KIAA0495 in multiple myeloma.
abstract: In multiple myeloma, a long non-coding RNA, KIAA0495 (alias PDAM/TP73-AS1), had been found progressively downregulated from normal plasma cell to benign monoclonal gammopathy of undetermined significance to symptomatic myeloma. Herein, by methylation-specific PCR, the putative KIAA0495 promoter was found unmethylated in all healthy controls (N = 14) but methylated in 50 % of myeloma cell lines (N = 10). KIAA0495 methylation was shown inversely correlated with KIAA0495 expression. However, KIAA0495 methylation was detected in none of both primary myeloma samples at diagnosis (N = 61) and at relapse/progression (N = 16). Collectively, despite frequently methylated in cell lines, KIAA0495 methylation appeared unimportant in the pathogenesis or progression of myeloma.
authors.affiliation: Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong. kwanyeung@graduate.hku.hk.
authors.forename: Kwan Yeung
authors.initials: KY
authors.lastname: Wong
authors.affiliation: Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong. zhhli@connect.hku.hk.
authors.forename: Zhenhai
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Surgery, Queen Mary Hospital, The University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong. zhxq@connect.hku.hk.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Surgery, Queen Mary Hospital, The University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong. gilberto@hku.hk.
authors.forename: Gilberto Ka Kit
authors.initials: GK
authors.lastname: Leung
authors.affiliation: Department of Paediatrics and Adolescent Medicine, Queen Mary Hospital, The University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong. gcfchan@hku.hk.
authors.forename: Godfrey Chi-Fung
authors.initials: GC
authors.lastname: Chan
authors.affiliation: Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong. jcschim@hku.hk.
authors.forename: Chor Sang
authors.initials: CS
authors.lastname: Chim
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019175:DNA Methylation / Q000235:genetics*; D018450:Disease Progression; D044127:Epigenesis, Genetic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology; D062085:RNA, Long Noncoding / Q000037:antagonists & inhibitors / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding; C000607535:long non-coding RNA KIAA0495, human