title: A simple LC-MS method for determination of cyasterone in rat plasma: application to a pilot pharmacokinetic study.
abstract: A simple, specific, and sensitive liquid chromatography-mass spectrometry (LC-MS) method for determination of cyasterone in rat plasma was developed in our laboratory. Cucurbitacin B was used as an internal standard (IS). After protein precipitation with twofold volume of acetonitrile, the analyte and IS were separated on a Luna C18 column (100 × 4.6 mm, i.d., 3.0 µm; Phenomenex) by isocratic elution with acetonitrile-water (80:20, v/v) as the mobile phase at a flow rate of 0.4 mL/min. An electrospray ionization source was applied and operated in the positive ion mode; selected ion monitoring scan mode was used for quantification, and the target ions m/z 543.3 for cyasterone and m/z 581.3 for IS were chosen. Good linearity was observed in the concentration range of 0.40-400 ng/mL for cyasterone in rat plasma. Intra-day and inter-day precision were both <7.4%. This method was proved to be suitable for pharmacokinetic studies after oral (5.0 mg/kg) or intravenous (0.5 mg/kg) administration of cyasterone in rats. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Eye Center, the Second Hospital of Jilin University, Changchun, 130041, People's Republic China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Li
authors.affiliation: Eye Center, the Second Hospital of Jilin University, Changchun, 130041, People's Republic China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Li
authors.affiliation: Eye Center, the Second Hospital of Jilin University, Changchun, 130041, People's Republic China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Eye Center, the Second Hospital of Jilin University, Changchun, 130041, People's Republic China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Eye Center, the Second Hospital of Jilin University, Changchun, 130041, People's Republic China.
authors.forename: Zaoxia
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Eye Center, the Second Hospital of Jilin University, Changchun, 130041, People's Republic China.
authors.forename: Guanfang
authors.initials: G
authors.lastname: Su
mesh_terms: D000818:Animals; D001682:Biological Availability; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D010865:Pilot Projects; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013265:Stigmasterol / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics
chemical_list: C002082:cyasterone; D013265:Stigmasterol
keywords: LC-MS; cyasterone; pharmacokinetic study; rat plasma