title: MiR-140-3p suppressed cell growth and invasion by downregulating the expression of ATP8A1 in non-small cell lung cancer.
abstract: MicroRNAs (miRNAs) as a class of small noncoding RNA molecules regulate the expression of targeted gene. The dysregulation of microRNAs is reported to be involved in carcinogenesis and tumor progression. Here, we identified miR-140-3p as a downregulated microRNA in most cancer tissues including lung cancer tissues, compared with their normal counterparts. MiR-140-3p was observed to perform its tumor suppressor function via its inhibition on cell growth, migration and invasion but its induction of cell apoptosis. Furthermore, the growth of non-small-cell lung cancer (NSCLC) cells in nude mouse models were suppressed by overexpression of miR-140-3p. ATP8A1 was demonstrated as a novel direct target of miR-140-3p using a luciferase assay. The increased level of intracellular ATP8A1 protein attenuated the inhibitor role of miR-140-3p in the growth and mobility of NSCLC cell. A regulation mechanism of miR-140-3p for the development and progression of NSCLC through downregulating the ATP8A1 expression was first discovered in the present study.
authors.affiliation: Shandong University School of Medicine, 44# Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.affiliation: Department of Radiation Oncology, Shandong Cancer Hospital and Institute, 440# Jiyan Road, Jinan, 250117, Shandong, People's Republic of China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Yao
authors.affiliation: Department of Radiation Oncology, Shandong Cancer Hospital and Institute, 440# Jiyan Road, Jinan, 250117, Shandong, People's Republic of China.
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Teng
authors.affiliation: Department of Radiation Oncology, Shandong Cancer Hospital and Institute, 440# Jiyan Road, Jinan, 250117, Shandong, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chai
authors.affiliation: Department of Radiation Oncology, Shandong Cancer Hospital and Institute, 440# Jiyan Road, Jinan, 250117, Shandong, People's Republic of China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Radiation Oncology, Shandong Cancer Hospital and Institute, 440# Jiyan Road, Jinan, 250117, Shandong, People's Republic of China. libaosheng01028@163.com.
authors.forename: Baosheng
authors.initials: B
authors.lastname: Li
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000818:Animals; D017209:Apoptosis; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000502:physiology*; D009361:Neoplasm Invasiveness; D049294:Phospholipid Transfer Proteins / Q000235:genetics*
chemical_list: D035683:MicroRNAs; C544302:Mirn140 microRNA, human; D049294:Phospholipid Transfer Proteins; C580722:ATP8A1 protein, human; D000251:Adenosine Triphosphatases
keywords: ATP8A1; Cell proliferation; Lung cancer; MiR-140-3p