title: Crystal structure of human nuclear pore complex component NUP43.
abstract: Nuclear pore complexes (NPC) form nuclear pores that cross the nuclear envelope and allow molecules to transport between the nucleus and the cytoplasm. We solved the crystal structure of human Nup43 (hNUP43), an important component in the Nup107 subcomplex of NPC. hNup43 adopts a seven-bladed β-propeller fold. We confirmed by ITC that neither human Nup37 (hNup37) nor human Nup133 (hNup133) interacts with hNup43. We demonstrated by analytical gel filtration that the human Nup85-Seh1L binary complex recruits hNup43 to form a ternary complex. Based on amino acid sequence analysis, we predicted the hNup85-hSeh1L binding surface of hNup43.
authors.affiliation: Structural Genomics Consortium, University of Toronto, 101 College St., Toronto, Ontario M5G 1L7, Canada; Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xu
authors.affiliation: Structural Genomics Consortium, University of Toronto, 101 College St., Toronto, Ontario M5G 1L7, Canada.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Structural Genomics Consortium, University of Toronto, 101 College St., Toronto, Ontario M5G 1L7, Canada.
authors.forename: Hao
authors.initials: H
authors.lastname: He
authors.affiliation: Structural Genomics Consortium, University of Toronto, 101 College St., Toronto, Ontario M5G 1L7, Canada.
authors.forename: Amy
authors.initials: A
authors.lastname: Wernimont
authors.affiliation: Structural Genomics Consortium, University of Toronto, 101 College St., Toronto, Ontario M5G 1L7, Canada.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Structural Genomics Consortium, University of Toronto, 101 College St., Toronto, Ontario M5G 1L7, Canada.
authors.forename: Peter
authors.initials: P
authors.lastname: Loppnau
authors.affiliation: Structural Genomics Consortium, University of Toronto, 101 College St., Toronto, Ontario M5G 1L7, Canada; Department of Physiology, University of Toronto, Toronto, Ontario M5S 1A8, Canada. Electronic address: jr.min@utoronto.ca.
authors.forename: Jinrong
authors.initials: J
authors.lastname: Min
mesh_terms: D001665:Binding Sites; D018360:Crystallography, X-Ray / Q000379:methods*; D006801:Humans; D015778:Minor Histocompatibility Antigens; D008958:Models, Molecular; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry* / Q000378:metabolism*; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary
chemical_list: D015778:Minor Histocompatibility Antigens; C440250:NUP133 protein, human; C000605772:NUP43 protein, human; C503214:NUP85 protein, human; D028861:Nuclear Pore Complex Proteins
keywords: Nup107 subcomplex; Nup43; Nup85; Seh1L; WD40 repeat