title: Neuro at the Nanoscale: Diffraction-Unlimited Imaging with STED Nanoscopy.
abstract: Recent breakthroughs in fluorescence microscopy have pushed spatial resolution well beyond the classical limit imposed by diffraction. As a result, the field of nanoscopy has emerged, and diffraction-unlimited resolution is becoming increasingly common in biomedical imaging applications. In this review, we recap the principles behind STED nanoscopy that allow imaging beyond the diffraction limit, and highlight both historical and recent advances made in the field of neuroscience as a result of this technology.
authors.affiliation: Department of Psychology and Program in Neuroscience , Bates College, Lewiston, Maine.(JBC)
authors.forename: Jason B
authors.initials: JB
authors.lastname: Castro
authors.affiliation: Department of Physics & Astronomy, Bates College, Lewiston, Maine. (TJG)
authors.forename: Travis J
authors.initials: TJ
authors.lastname: Gould
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000737:chemistry / Q000502:physiology; D004331:Drosophila melanogaster / Q000502:physiology / Q000648:ultrastructure; D015870:Gene Expression; D006624:Hippocampus / Q000502:physiology / Q000648:ultrastructure; D008027:Light; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D059906:Neuroimaging / Q000295:instrumentation / Q000379:methods*; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure*; D018377:Neurotransmitter Agents / Q000737:chemistry / Q000502:physiology; D013131:Spine / Q000502:physiology / Q000648:ultrastructure; D013569:Synapses / Q000502:physiology / Q000648:ultrastructure; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: C510146:BRP protein, Drosophila; D029721:Drosophila Proteins; D018377:Neurotransmitter Agents
keywords: Dendrite; Fluorescence; Nanoscopy; Neuroimaging; Neuron; Neurotransmitter Release; STED Microscopy; Spine; Super-resolution; Synapse