title: A novel functional glucose transporter in the white shrimp Litopenaeus vannamei -LvGLUT2- is up-regulated during hypoxia in hepatopancreas.
abstract: In hypoxia conditions, the white shrimp Litopenaeus vannamei shifts its energetic metabolism from aerobic to anaerobic, requiring more glucose uptake into the cells by GLUT proteins. We here report a novel glucose transporter in shrimp. The Lvglut2 cDNA is 2473 bp-long containing an ORF of 1458 bp encoding 486 amino acid residues. The deduced protein has the features of a facilitative sugar transporter. The Lvglut2 gene product tagged with GFP was expressed in the cell membrane of Xenopus oocytes. In the same expression system, untagged LvGLUT2 resulted to be a bidirectional glucose transporter that functions moving glucose down its concentration gradient in and out of the cell. Lvglut2 mRNA is expressed in hepatopancreas while in muscle and gills it was not detected. Hypoxia up-regulates the expression of Lvglut2 transcripts in hepatopancreas. These results provide a better understanding of facilitative glucose transporters and gene regulation during hypoxia in crustaceans.
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo. A.C., Hermosillo, Sonora, Mexico.
authors.forename: José A
authors.initials: JA
authors.lastname: Martínez-Quintana
authors.affiliation: Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Kikuta
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo. A.C., Hermosillo, Sonora, Mexico.
authors.forename: Monserrath
authors.initials: M
authors.lastname: Felix-Portillo
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo. A.C., Hermosillo, Sonora, Mexico.
authors.forename: Alma B
authors.initials: AB
authors.lastname: Peregrino-Uriarte
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo. A.C., Hermosillo, Sonora, Mexico. Electronic address: gyepiz@ciad.mx.
authors.forename: Gloria
authors.initials: G
authors.lastname: Yepiz-Plascencia
mesh_terms: D000595:Amino Acid Sequence; D000693:Anaerobiosis; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics* / Q000378:metabolism; D043143:Hepatopancreas / Q000378:metabolism; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics / Q000502:physiology*; D016415:Sequence Alignment; D015854:Up-Regulation
chemical_list: D060829:Arthropod Proteins; D018076:DNA, Complementary; D051246:Glucose Transport Proteins, Facilitative
keywords: Crustaceans; GLUT; Glucose transporter; Hypoxia; Shrimp; Xenopus oocytes