title: Recombinant human thrombopoietin promotes hematopoietic reconstruction after severe whole body irradiation.
abstract: Recombinant human thrombopoietin (rHuTPO) is a drug that is used clinically to promote megakaryocyte and platelet generation. Here, we report the mitigative effect of rHuTPO (administered after exposure) against severe whole body irradiation in mice. Injection of rHuTPO for 14 consecutive days following exposure significantly improved the survival rate of lethally irradiated mice. RHuTPO treatment notably increased bone marrow cell density and LSK cell numbers in the mice after sub-lethal irradiation primarily by promoting residual HSC proliferation. In lethally irradiated mice with hematopoietic cell transplantation, rHuTPO treatment increased the survival rate and enhanced hematopoietic cell engraftment compared with the placebo treatment. Our observations indicate that recombinant human TPO might have a therapeutic role in promoting hematopoietic reconstitution and HSC engraftment.
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.|School of Life Science and Bio Pharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.|South China Research Center for Stem Cell &Regenerative Medicine, AMMS, Guangzhou 510005, China.
authors.forename: Bowen
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.|South China Research Center for Stem Cell &Regenerative Medicine, AMMS, Guangzhou 510005, China.
authors.forename: Sihan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.|South China Research Center for Stem Cell &Regenerative Medicine, AMMS, Guangzhou 510005, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.|South China Research Center for Stem Cell &Regenerative Medicine, AMMS, Guangzhou 510005, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.|South China Research Center for Stem Cell &Regenerative Medicine, AMMS, Guangzhou 510005, China.
authors.forename: Jingxue
authors.initials: J
authors.lastname: Wang
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.|South China Research Center for Stem Cell &Regenerative Medicine, AMMS, Guangzhou 510005, China.
authors.forename: Zeng
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.|South China Research Center for Stem Cell &Regenerative Medicine, AMMS, Guangzhou 510005, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.|South China Research Center for Stem Cell &Regenerative Medicine, AMMS, Guangzhou 510005, China.
authors.forename: Xiuyuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.|South China Research Center for Stem Cell &Regenerative Medicine, AMMS, Guangzhou 510005, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: He
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.|South China Research Center for Stem Cell &Regenerative Medicine, AMMS, Guangzhou 510005, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Life Science and Bio Pharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Huanzhang
authors.initials: H
authors.lastname: Xia
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.|South China Research Center for Stem Cell &Regenerative Medicine, AMMS, Guangzhou 510005, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.|South China Research Center for Stem Cell &Regenerative Medicine, AMMS, Guangzhou 510005, China.
authors.forename: Xuetao
authors.initials: X
authors.lastname: Pei
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D016026:Bone Marrow Transplantation; D004195:Disease Models, Animal; D006410:Hematopoiesis / Q000187:drug effects* / Q000528:radiation effects*; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D008297:Male; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D013926:Thrombopoietin / Q000494:pharmacology*; D013997:Time Factors; D014916:Whole-Body Irradiation / Q000009:adverse effects* / Q000401:mortality
chemical_list: D011994:Recombinant Proteins; D013926:Thrombopoietin