title: Prophylactic and Therapeutic Effects of Oleuropein on Reperfusion-Induced Arrhythmia in Anesthetized Rat.
abstract: BACKGROUND
  This study was conducted to reveal that whether i.v. injection of oleuropein, the most potent polyphenolic antioxidant in olive leaf, has any effect on the magnitude of reperfusion arrhythmia in anesthetized rats or not.
  
  
  METHODS
  Eighty male Wistar rats were divided into 8 groups of 10 each: groups 1 and 5 were assigned as the prophylactic and treatment control groups, groups 2 and 6 as the prophylactic and treatment groups with lidocaine (10 mg/kg), groups 3 and 4 as the prophylactic groups with 10 and 50 mg/kg oleuropein (i.v.), and groups 7 and 8 as the treatment groups with 10 and 50 mg/kg oleuropein (i.v.), respectively. Reperfusion injury was induced by 5-min regional ischemia and 15-min reperfusion of left anterior descending coronary artery. Heart rate, blood pressure, and electrocardiogram were monitored throughout the procedure.
  
  
  RESULTS
  blood pressure was significantly decreased by infusion of 50 mg/kg oleuropein in groups 4 and 8, but unlike the lidocaine as a standard anti-arrhythmic drug in groups 2 and 5 had not significant effect on heart rate. The onset of arrhythmia in groups received oleuropein (groups 3, 4, 7, and 8) was significantly delayed. The mortality rate due to irreversible ventricular fibrillation was also significantly reduced in groups 3, 4, 7, and 8. The effect of lidocaine in groups 2 and 5 was more potent than that in oleuropein group.
  
  
  CONCLUSIONS
  These findings indicate that i.v. injection of oleuropein possibly through its antioxidant activity reduces the magnitude of reperfusion-induced arrhythmia.
authors.affiliation: Heart Center, Lorestan University of Medical Sciences, Khoramabad, Iran.
authors.forename: Babak
authors.initials: B
authors.lastname: Baharvand
authors.affiliation: Dept. of Physiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Mansour
authors.initials: M
authors.lastname: Esmailidehaj
authors.affiliation: Dept. of Physiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Jamileh
authors.initials: J
authors.lastname: Alihosaini
authors.affiliation: Dept. of Physiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Shirin
authors.initials: S
authors.lastname: Bajoovand
authors.affiliation: Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Saeedeh
authors.initials: S
authors.lastname: Esmailidehaj
authors.affiliation: Dept. of Physiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Zeynab
authors.initials: Z
authors.lastname: Hafizie
mesh_terms: D000771:Anesthesia, Intravenous / Q000379:methods; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000008:administration & dosage; D001145:Arrhythmias, Cardiac / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D057888:Iridoid Glucosides; D039823:Iridoids / Q000008:administration & dosage*; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology; D029592:Oleaceae; D065129:Pre-Exposure Prophylaxis / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D000889:Anti-Arrhythmia Agents; D057888:Iridoid Glucosides; D039823:Iridoids; C002769:oleuropein
keywords: Arrhythmia; Oleuropein; Rats; Reperfusion