title: Reversible Regulation of Catalytic Activity of Gold Nanoparticles with DNA Nanomachines.
abstract: Reversible catalysis regulation has gained much attention and traditional strategies utilized reversible ligand coordination for switching catalyst's conformations. However, it remains challenging to regulate the catalytic activity of metal nanoparticle-based catalysts. Herein, we report a new DNA nanomachine-driven reversible nano-shield strategy for circumventing this problem. The basic idea is based on the fact that the conformational change of surface-attached DNA nanomachines will cause the variation of the exposed surface active area on metal nanoparticles. As a proof-of-concept study, we immobilized G-rich DNA strands on gold nanoparticles (AuNPs) which have glucose oxidase (GOx) like activity. Through the reversible conformational change of the G-rich DNA between a flexible single-stranded form and a compact G-quadruplex form, the catalytic activity of AuNPs has been regulated reversibly for several cycles. This strategy is reliable and robust, which demonstrated the possibility of reversibly adjusting catalytic activity with external surface coverage switching, rather than coordination interactions.
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical College, Wenzhou 325035, Zhejiang, China.|Division of Physical Biology &Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Division of Physical Biology &Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Jia
authors.affiliation: Division of Physical Biology &Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Dun
authors.initials: D
authors.lastname: Pan
authors.affiliation: Division of Physical Biology &Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical College, Wenzhou 325035, Zhejiang, China.
authors.forename: Jimin
authors.initials: J
authors.lastname: Gao
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical College, Wenzhou 325035, Zhejiang, China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Lu
authors.affiliation: Division of Physical Biology &Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.|Department of Endodontics, 9th People's Hospital, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University, Shanghai 200011, China.
authors.forename: Jiye
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Endodontics, 9th People's Hospital, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University, Shanghai 200011, China.
authors.forename: Zisheng
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Division of Physical Biology &Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Huajie
authors.initials: H
authors.lastname: Liu
mesh_terms: D002384:Catalysis; D004247:DNA / Q000378:metabolism*; D054856:G-Quadruplexes; D005949:Glucose Oxidase / Q000378:metabolism*; D006046:Gold / Q000737:chemistry / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D013499:Surface Properties
chemical_list: D006046:Gold; D004247:DNA; D005949:Glucose Oxidase