title: Specific Pathways of Dietary Methylmercury and Inorganic Mercury Determined by Mercury Speciation and Isotopic Composition in Zebrafish (Danio rerio).
abstract: An original approach is proposed to investigate inorganic (iHg) and methylmercury (MeHg) trophic transfer and fate in a model fish, Danio rerio, by combining natural isotopic fractionation and speciation. Animals were exposed to three different dietary conditions: (1) 50 ng Hg g(-1), 80% as MeHg; (2) diet enriched in MeHg 10,000 ng Hg g(-1), 95% as MeHg, and (3) diet enriched in iHg 10,000 ng Hg g(-1), 99% as iHg. Harvesting was carried out after 0, 7, 25, and 62 days. Time-dependent Hg species distribution and isotopic fractionation in fish organs (muscle, brain, liver) and feces, exhibited different patterns, as a consequence of their dissimilar metabolization. The rapid isotopic re-equilibration to the new MeHg-food source reflects its high bioaccumulation rate. Relevant aspects related to Hg excretion are also described. This study confirms Hg isotopic fractionation as a powerful tool to investigate biological processes, although its deconvolution and fully understanding is still a challenge.
authors.affiliation: Laboratoire de Chimie Analytique, Bio-Inorganique et Environnement, Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux (IPREM), CNRS-UPPA-UMR-5254, Hélioparc , 2 Avenue du Président Pierre Angot, F-64053 Pau, France.
authors.forename: Caiyan
authors.initials: C
authors.lastname: Feng
authors.affiliation: Laboratoire de Chimie Analytique, Bio-Inorganique et Environnement, Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux (IPREM), CNRS-UPPA-UMR-5254, Hélioparc , 2 Avenue du Président Pierre Angot, F-64053 Pau, France.
authors.forename: Zoyne
authors.initials: Z
authors.lastname: Pedrero
authors.affiliation: Université de Bordeaux , EPOC, UMR CNRS 5805, F-33120 Arcachon, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Gentès
authors.affiliation: Laboratoire de Chimie Analytique, Bio-Inorganique et Environnement, Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux (IPREM), CNRS-UPPA-UMR-5254, Hélioparc , 2 Avenue du Président Pierre Angot, F-64053 Pau, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Barre
authors.affiliation: Laboratoire de Chimie Analytique, Bio-Inorganique et Environnement, Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux (IPREM), CNRS-UPPA-UMR-5254, Hélioparc , 2 Avenue du Président Pierre Angot, F-64053 Pau, France.
authors.forename: Marina
authors.initials: M
authors.lastname: Renedo
authors.affiliation: Laboratoire de Chimie Analytique, Bio-Inorganique et Environnement, Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux (IPREM), CNRS-UPPA-UMR-5254, Hélioparc , 2 Avenue du Président Pierre Angot, F-64053 Pau, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Tessier
authors.affiliation: Laboratoire de Chimie Analytique, Bio-Inorganique et Environnement, Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux (IPREM), CNRS-UPPA-UMR-5254, Hélioparc , 2 Avenue du Président Pierre Angot, F-64053 Pau, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Berail
authors.affiliation: Université de Bordeaux , EPOC, UMR CNRS 5805, F-33120 Arcachon, France.
authors.forename: Régine
authors.initials: R
authors.lastname: Maury-Brachet
authors.affiliation: Université de Bordeaux , EPOC, UMR CNRS 5805, F-33120 Arcachon, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Mesmer-Dudons
authors.affiliation: Université de Bordeaux , EPOC, UMR CNRS 5805, F-33120 Arcachon, France.
authors.forename: Magalie
authors.initials: M
authors.lastname: Baudrimont
authors.affiliation: Université de Bordeaux , EPOC, UMR CNRS 5805, F-33120 Arcachon, France.
authors.forename: Alexia
authors.initials: A
authors.lastname: Legeay
authors.affiliation: Observatoire Midi-Pyrénées, Laboratoire de Géosciences Environnement Toulouse, Université Paul Sabatier Toulouse III , 14 avenue Edouard Belin, 31400 Toulouse, France.|GET, IRD , F-31400 Toulouse, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Maurice
authors.affiliation: Université de Bordeaux , EPOC, UMR CNRS 5805, F-33120 Arcachon, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Gonzalez
authors.affiliation: Laboratoire de Chimie Analytique, Bio-Inorganique et Environnement, Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux (IPREM), CNRS-UPPA-UMR-5254, Hélioparc , 2 Avenue du Président Pierre Angot, F-64053 Pau, France.
authors.forename: David
authors.initials: D
authors.lastname: Amouroux
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D004032:Diet; D004784:Environmental Monitoring; D005243:Feces / Q000737:chemistry; D008099:Liver / Q000378:metabolism; D008628:Mercury / Q000032:analysis*; D008629:Mercury Isotopes; D008767:Methylmercury Compounds / Q000032:analysis*; D009132:Muscles / Q000378:metabolism; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D008629:Mercury Isotopes; D008767:Methylmercury Compounds; D008628:Mercury