title: Phenylmethanesulfonyl fluoride pretreatment stabilizes plasma lipidome in lipidomic and metabolomic analysis.
abstract: Though it is standard practice to test the stability of analytes in the matrix for routine bioanalytical method, stability evaluation is always impractical and skipped in untargeted lipidomic and metabolomic analysis because analytes in these studies are enormous, diverse and sometimes unknown. Lipidome represents a major class of plasma metabolome and shows great potential to be diagnostic and prognostic biomarkers. However, lipidome also faces stability problems because plasma contains kinds of lipid degradation enzyme. Here, using liquid chromatography time of flight mass spectrometry based lipidomic methodology, plasma levels of various lipids including triglyceride (TG), diglyceride (DG), free fatty acid (FFA), phosphatidylethanolamine (PE) phosphatidylcholine (PC), lyso-phosphatidylcholine (LPC), lyso-phosphatidylethanolamine (LPE), and sphingomyelin (SM) were dynamically determined within 4 h at ambient temperature. In mouse and rat plasma, the levels of most TG, DG, PC and PE species significantly decreased with respect to time, whereas those of LPC, LPE and FFA significantly increased with respect to time. However, such changes did not occur in human plasma, thus indicating hepatic lipase and esterase might involve in the species-specified degradation of lipid classes in plasma. Phenylmethanesulfonyl fluoride (PMSF) pretreatment prevented such lipidome instability in mouse plasma. The results suggested the instability of plasma lipidome should be highly concerned, and the enhancement of ex vivo stability of plasma lipidome could enable more reliable clinical translation of lipidomic data for biomarker discovery.
authors.affiliation: Shanghai Jiao Tong University School of Medicine, Institute of Medical Sciences, Department of Pharmacology, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Shanghai Jiao Tong University School of Medicine, Institute of Medical Sciences, Department of Pharmacology, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Gu
authors.affiliation: Shanghai Jiao Tong University School of Medicine, Institute of Medical Sciences, Department of Pharmacology, China.
authors.forename: Huahua
authors.initials: H
authors.lastname: Song
authors.affiliation: Shanghai Jiao Tong University School of Medicine, Institute of Medical Sciences, Department of Pharmacology, China.
authors.forename: Qingxiang
authors.initials: Q
authors.lastname: Song
authors.affiliation: Shanghai Jiao Tong University School of Medicine, Institute of Medical Sciences, Department of Pharmacology, China. Electronic address: shellygao1@sjtu.edu.cn.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Gao
authors.affiliation: Shanghai Jiao Tong University School of Medicine, Institute of Medical Sciences, Department of Pharmacology, China. Electronic address: luyangssmu@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Shanghai Jiao Tong University School of Medicine, Institute of Medical Sciences, Department of Pharmacology, China. Electronic address: hongzhuan_chen@hotmail.com.
authors.forename: Hongzhuan
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D005230:Fatty Acids, Nonesterified / Q000097:blood; D006801:Humans; D008055:Lipids / Q000097:blood*; D013058:Mass Spectrometry; D055442:Metabolome; D055432:Metabolomics; D051379:Mice; D008813:Mice, Inbred ICR; D010664:Phenylmethylsulfonyl Fluoride / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000097:blood; D010714:Phosphatidylethanolamines / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D013109:Sphingomyelins / Q000097:blood; D013696:Temperature; D014280:Triglycerides / Q000097:blood
chemical_list: D015415:Biomarkers; D005230:Fatty Acids, Nonesterified; D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D013109:Sphingomyelins; D014280:Triglycerides; D010664:Phenylmethylsulfonyl Fluoride
keywords: Hepatic lipase and esterase; Lipidomics; Mass spectrometry; Metabolomics; Phenylmethanesulfonyl fluoride pretreatment; Plasma lipidome stability