title: Rosa canina L.--new possibilities for an old medicinal herb.
abstract: Health beneficial properties of Rosa canina species are mainly attributed to rose hips, while the leaves are usually discarded as waste. In the present study we investigated chemical constituents as well as antimicrobial and antibiofilm potential of R. canina methanolic leaf extract. Chemical analysis showed that dominant phenolic compounds are quercetin and isorhamnetin derivatives (isoquercetin and isorhamnetin-3-O-rutinoside). Among the tested bacteria, Pseudomonas aeruginosa and Salmonella typhimurium were the most susceptible to the activity of R. canina leaf extract with MIC and MBC values both 0.009 mg mL(-1). For most of the bacterial strains investigated the extract showed significantly higher activity compared to the used standard compounds streptomycin and ampicillin. Also the tested extract powerfully inhibited in vitro biofilm growth in Pseudomonas aeruginosa, Staphylococcus aureus, Leishmania monocytogenes and Escherichia coli at sub MIC-levels. With concentrations equal to 1/4 and 1/8 of MIC values biofilm growth of P. aeruginosa and E. coli was inhibited by 90%. The obtained results are significant for a wider and efficient use of R. canina leaves.
authors.affiliation: Institute for Medicinal Plant Research "Dr. Josif Pančić", Tadeuša Košćuška 1, 11000 Belgrade, Serbia. jzivkovic@mocbilja.rs.
authors.forename: Jelena
authors.initials: J
authors.lastname: Živković
authors.affiliation: Department of Plant Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia.
authors.forename: Dejan
authors.initials: D
authors.lastname: Stojković
authors.affiliation: Department of Plant Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia.
authors.forename: Jovana
authors.initials: J
authors.lastname: Petrović
authors.affiliation: Institute for Medicinal Plant Research "Dr. Josif Pančić", Tadeuša Košćuška 1, 11000 Belgrade, Serbia. jzivkovic@mocbilja.rs.
authors.forename: Gordana
authors.initials: G
authors.lastname: Zdunić
authors.forename: Jasmina
authors.initials: J
authors.lastname: Glamočlija
authors.affiliation: Department of Plant Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia.
authors.forename: Jasna
authors.initials: J
authors.lastname: Glamočlija
authors.affiliation: Department of Plant Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia.
authors.forename: Marina
authors.initials: M
authors.lastname: Soković
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D004187:Disaccharides / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology; D007891:Leishmania / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D027826:Rosa / Q000737:chemistry*; D012486:Salmonella typhimurium / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D004187:Disaccharides; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; C515815:isorhamnetin 3-O-rutinoside