title: Systematic screening and characterization of glycosides in tobacco leaves by liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry using neutral loss scan and product ion scan.
abstract: Glycosides in tobacco leaves are highly important aromatic precursors. It is necessary to reveal glycosides in tobacco leaves to improve tobacco planting and processing. This study describes a method for the systematic screening of glycosides in tobacco leaves by liquid chromatography with tandem mass spectrometry. Although glycosides contain numerous aglycones, the number of glycans is limited. Based on a screening table of glycans designed for neutral loss scan, glycosides with different aglycones were systematically screened out. Then, the MS(2) fragment spectra of scanned glycosides were further obtained using product ion scan. By comparison with the spectra in online tandem mass spectral databases, reported references, and verification by commercial standards, 64 glycosides were detected, including 39 glycosides linked with monosaccharides, 18 glycosides linked with disaccharides and 7 glycosides linked with trisaccharides. It is noteworthy that glycosides linked with trisaccharides have previously been rarely reported in tobacco. This method appears to be a useful tool for the systematic screening and characterization of glycosides in tobacco and can potentially be applied to other plants.
authors.affiliation: Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.affiliation: Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
authors.forename: Fuwei
authors.initials: F
authors.lastname: Xie
mesh_terms: D001274:Atmospheric Pressure; D002623:Chemistry Techniques, Analytical / Q000379:methods* / Q000592:standards; D002853:Chromatography, Liquid; D006027:Glycosides / Q000737:chemistry*; D009005:Monosaccharides / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D014026:Nicotiana / Q000737:chemistry*; D019460:Tobacco Industry / Q000379:methods*
chemical_list: D006027:Glycosides; D009005:Monosaccharides
keywords: Atmospheric pressure chemical ionization; Glycosides; Liquid chromatography; Tandem mass spectrometry; Tobacco