title: Effects of octahedral molecular sieve on treatment performance, microbial metabolism, and microbial community in expanded granular sludge bed reactor.
abstract: This study evaluated the effects of synthesized octahedral molecular sieve (OMS-2) nanoparticles on the anaerobic microbial community in a model digester, expanded granular sludge bed (EGSB) reactor. The addition of OMS-2 (0.025 g/L) in the EGSB reactors resulted in an enhanced operational performance, i.e., COD removal and biogas production increased by 4% and 11% respectively, and effluent volatile fatty acid (VFA) decreased by 11% relative to the control group. The Biolog EcoPlate™ test was employed to investigate microbial metabolism in the EGSB reactors. Results showed that OMS-2 not only increased the microbial metabolic level but also significantly changed the community level physiological profiling of the microorganisms. The Illumina MiSeq high-throughput sequencing of 16S rRNA gene indicated OMS-2 enhanced the microbial diversity and altered the community structure. The largest bacterial genus Lactococcus, a lactic acid bacterium, reduced from 29.3% to 20.4% by abundance in the presence of 0.25 g/L OMS-2, which may be conducive to decreasing the VFA production and increasing the microbial diversity. OMS-2 also increased the quantities of acetogenic bacteria and Archaea, and promoted the acetogenesis and methanogenesis. The X-ray photoelectron spectroscopy illustrated that Mn(IV)/Mn(III) with high redox potential in OMS-2 were reduced to Mn(II) in the EGSB reactors; this in turn affected the microbial community.
authors.affiliation: School of Environmental Engineering, Wuhan Textile University, Wuhan 430073, China. Electronic address: leopan0724@189.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Pan
authors.affiliation: School of Environmental Engineering, Wuhan Textile University, Wuhan 430073, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Xu
authors.affiliation: School of Environmental Engineering, Wuhan Textile University, Wuhan 430073, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Xia
authors.affiliation: School of Environmental Engineering, Wuhan Textile University, Wuhan 430073, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Radiation Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Guo
authors.initials: G
authors.lastname: Chen
authors.affiliation: School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Meyer
authors.affiliation: Environmental Engineering Program, Department of Civil Engineering, Auburn University, Auburn, AL 36849, USA.
authors.forename: Dongye
authors.initials: D
authors.lastname: Zhao
authors.affiliation: School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
authors.forename: Ching-Hua
authors.initials: CH
authors.lastname: Huang
authors.affiliation: Collaborative Innovation Center of Water Quality Safety and Protection in Pearl River Delta, Guangzhou University, Guangzhou 510006, China.
authors.forename: Qihang
authors.initials: Q
authors.lastname: Wu
authors.affiliation: School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA. Electronic address: jie.fu@ce.gatech.edu.
authors.forename: Jie
authors.initials: J
authors.lastname: Fu
mesh_terms: D001105:Archaea / Q000235:genetics / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology; D005232:Fatty Acids, Volatile / Q000378:metabolism; D017895:Manganese Compounds; D053768:Metal Nanoparticles; D010087:Oxides; D056951:Photoelectron Spectroscopy; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D056804:Biofuels; D005232:Fatty Acids, Volatile; D017895:Manganese Compounds; D010087:Oxides; D012336:RNA, Ribosomal, 16S; D012722:Sewage; C016552:manganese dioxide
keywords: Anaerobic digestion; High-throughput sequencing; Lactic acid bacteria; Manganese; Microbial metabolism; Nanoparticles