title: Preparation and in vitro evaluation of Nystatin micro emulsion based gel.
abstract: Nystatin is a polyene antimycotic obtained from Streptomyces noursei used in the treatment of topical and transdermal fungal infection. Nystatin is nearly insoluble in water (<0.1) and it is amphoteric in nature. The aim of the present study was to design and develop Nystatin micro emulsion based gel for efficient delivery of drug to the skin by water titration method. The Pseudoternary phase diagrams 1:2, 1:1 and 2:1 were constructed by water titration method. Micro emulsion based gel was prepared by using oleic acid, Tween 20, propylene glycol as an oil phase, surfactant and cosurfactant respectively. Cabopol 940 was used as a gelling agent. In vitro evaluation of micro emulsion based gel was done for pH, Viscosity, spreadability and droplet size. Micro emulsion based gel showed greater antifungal activity against Candida albicansas compared to control formulations. In vitro drug release studies were conducted for micro emulsion based gel and control formulation using Franz diffusion cell. Drug penetration through synthetic skin followed Zero order model as the values for R2 higher in case of zero order equation. The optimized micro emulsion based gel was found to be stable and showed no physical changes when exposed to different temperatures for a period of 4 week. The results indicated that the micro emulsion based gel system studied would be a promising tool for enhancing the percutaneous delivery of Nystatin.
authors.affiliation: Riphah Institute of Pharmaceutical Sciences, Riphah International University, Lahore, Pakistan.
authors.forename: Iram
authors.initials: I
authors.lastname: Maqsood
authors.affiliation: University of Veterinary & Animal Sciences, Abdul Qadir Jillani Road, Lahore, Pakistan.
authors.forename: Muhammad Irfan
authors.initials: MI
authors.lastname: Masood
authors.affiliation: Department of Pharmacy, University of Sargodha, Sargodha, Pakistan.
authors.forename: Sajid
authors.initials: S
authors.lastname: Bashir
authors.affiliation: University of Veterinary & Animal Sciences, Abdul Qadir Jillani Road, Lahore, Pakistan.
authors.forename: Hafiz Muhammad Awais
authors.initials: HM
authors.lastname: Nawaz
authors.affiliation: University of Veterinary & Animal Sciences, Abdul Qadir Jillani Road, Lahore, Pakistan.
authors.forename: Aftab Ahmad
authors.initials: AA
authors.lastname: Anjum
authors.affiliation: University of Veterinary & Animal Sciences, Abdul Qadir Jillani Road, Lahore, Pakistan.
authors.forename: Iram
authors.initials: I
authors.lastname: Shahzadi
authors.affiliation: Department of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Ahmad
authors.affiliation: Department of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Imran Masood
authors.initials: IM
authors.lastname: Imran Masood
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry*; D004355:Drug Stability; D004655:Emulsions; D005782:Gels; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009761:Nystatin / Q000737:chemistry* / Q000494:pharmacology; D012995:Solubility; D014783:Viscosity
chemical_list: D000935:Antifungal Agents; D004655:Emulsions; D005782:Gels; D009761:Nystatin