title: Detection of Aleutian disease virus by loop-mediated isothermal amplification.
abstract: In this study, a loop-mediated isothermal amplification (LAMP) assay was developed and optimized for the detection of Aleutian disease virus (ADV) in minks. The amplification could be completed within 45 min under isothermal condition by employing a set of six ADV genome-specific primers. The amplification results could be visualized directly with the naked eye by using fluorescent dye. Comparative experiments showed that the LAMP assay is superior to conventional polymerase chain reaction for the detection of both experimental and field samples. Results of current study indicated that the LAMP assay is a rapid and reliable technique for routine diagnosis of ADV infection in minks.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030 Heilongjiang China ; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001 Heilongjiang China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001 Heilongjiang China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001 Heilongjiang China.
authors.forename: Shouping
authors.initials: S
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001 Heilongjiang China.
authors.forename: Jiaoer
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001 Heilongjiang China.
authors.forename: Xijun
authors.initials: X
authors.lastname: He
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030 Heilongjiang China.
authors.forename: Shimin
authors.initials: S
authors.lastname: Zheng
keywords: Aleutian disease virus; Diagnosis; Loop-mediated isothermal amplification