title: Antibodies to P-selectin glycoprotein ligand-1 block dendritic cell-mediated enterovirus 71 transmission and prevent virus-induced cells death.
abstract: P-selectin glycoprotein ligand-1 (PSGL-1) has been proved to serve as the functional receptor for enterovirus 71 (EV71). We found the abundant expression of PSGL-1 on monocyte-derived dendritic cells (MDDCs). However, we have previously demonstrated that MDDCs did not support efficient replication of EV71. Dendritic cells (DCs) have been described to be subverted by various viruses including EV71 for viral dissemination, we thus explore the potential contribution of PSGL-1 on DC-mediated EV71 transmission. We found that the cell-surface-expressing PSGL-1 on MDDCs mediated EV71 binding, and intriguingly, these loaded-viruses on MDDCs could be transferred to encountered target cells; Prior-treatment with PSGL-1 antibodies or interference with PSGL-1 expression diminished MDDC-mediated EV71 transfer and rescued virus-induced cell death. Our data uncover a novel role of PSGL-1 in DC-mediated EV71 spread, and provide an insight into blocking primary EV71 infection.
authors.affiliation: a Jiangsu Key Laboratory of Infection and Immunity; Institutes of Biology and Medical Sciences; Soochow University ; Suzhou , China.|b Key Laboratory of Molecular Virology and Immunology; Institute Pasteur of Shanghai; Chinese Academy of Sciences ; Shanghai , China.
authors.forename: Xiao-Xin
authors.initials: XX
authors.lastname: Ren
authors.affiliation: b Key Laboratory of Molecular Virology and Immunology; Institute Pasteur of Shanghai; Chinese Academy of Sciences ; Shanghai , China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Li
authors.affiliation: a Jiangsu Key Laboratory of Infection and Immunity; Institutes of Biology and Medical Sciences; Soochow University ; Suzhou , China.
authors.forename: Si-Dong
authors.initials: SD
authors.lastname: Xiong
authors.affiliation: b Key Laboratory of Molecular Virology and Immunology; Institute Pasteur of Shanghai; Chinese Academy of Sciences ; Shanghai , China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Huang
authors.affiliation: b Key Laboratory of Molecular Virology and Immunology; Institute Pasteur of Shanghai; Chinese Academy of Sciences ; Shanghai , China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
authors.affiliation: b Key Laboratory of Molecular Virology and Immunology; Institute Pasteur of Shanghai; Chinese Academy of Sciences ; Shanghai , China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Wang
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D016923:Cell Death / Q000276:immunology; D002460:Cell Line; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000821:virology*; D029821:Enterovirus A, Human / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D054884:Host-Pathogen Interactions; D006801:Humans; D019169:Jurkat Cells; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000378:metabolism; D034741:RNA, Small Interfering; D011991:Receptors, Virus / Q000378:metabolism; D053585:Virus Attachment / Q000187:drug effects
chemical_list: D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein; D034741:RNA, Small Interfering; D011991:Receptors, Virus
keywords: Dendritic cell; P-selectin glycoprotein ligand-1; enterovirus 71; viral transmission