title: Antibacterial activity of Citrus limonum fruit juice extract.
abstract: The fruit juice extract of Citrus limonum was investigated for antibacterial activity. The antibacterial activity of the extract on ten strains of bacteria was determined by both agar well diffusion and macro-broth dilution methods. The extract was variously bacteriostatic and bactericidal against Bacillussubtilis ATCC 6051, Staphylococcus aureus ATCC 12600, Escherichia coli ATCC 11775, Pseudomonas aeruginosa ATCC 10145 as well as locally isolated clinical strains of the above bacteria and Salmonella kintambo (Human: 13, 23: mt:-), Salmonella typhi and Proteus sp. The MICs ranged from 0.78 mg/ml to 50mg/ml; MBCs, 25.0mg/ml to >100mg/ml and MBC/MIC ratios 2.0 to >16.0. These results provide scientific justification for the medicinal use of Citrus limonum fruit juice by Nigerian herbalists in the treatment of diseases in which strains of the test organisms have been implicated as etiologic agents.
authors.affiliation: Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria / GenØk-Centre for Biosafety, Tromsø Science Park, Tromsø, Norway / Institute of Medical Biology, University of Tromsø, Tromsø, Norway.
authors.forename: Malachy Ifeanyi
authors.initials: MI
authors.lastname: Okeke
authors.affiliation: Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria / GenØk-Centre for Biosafety, Tromsø Science Park, Tromsø, Norway / Institute of Medical Biology, University of Tromsø, Tromsø, Norway.
authors.forename: Arinze Stanley
authors.initials: AS
authors.lastname: Okoli
authors.affiliation: Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria.
authors.forename: Edith Nneka
authors.initials: EN
authors.lastname: Eze
authors.affiliation: Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria.
authors.forename: Grace Chinwe
authors.initials: GC
authors.lastname: Ekwume
authors.affiliation: Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria.
authors.forename: Evangelin Uchena
authors.initials: EU
authors.lastname: Okosa
authors.affiliation: Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria.
authors.forename: Christian Ukwuoma
authors.initials: CU
authors.lastname: Iroegbu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002957:Citrus; D000067030:Fruit and Vegetable Juices; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts