title: [Protection of Shenlian extracts to PM2.5 infected RAW 264.7 cell damage].
abstract: The aim of this research is to investigate the protection of PM2.5 infected RAW264.7 cell by traditional Chinese medicine (TCM)--Shenlian(SL) extracts and to establish the damage model. We use cell growth, cell damage and oxidative stress related markers, and inflammatory cytokines as observation index to evaluate the protection of PM2.5 infected RAW264.7 by SL extract. The results showed that 50 mg x L(-1) PM2.5 could cause cell particle deposition, inhibit the growth of cells, and significantly increase the cell supernatant of LDH, NO release quantity and intracellular reactive oxygen species (ROS) level during 4 h and 24 h. In the intervention of SL extract 50, 25, 10 mg x L(-1), the particle deposition of RAW264.7 cells, cell supernatant of LDH, NO, IL(-1) beta release, MCP-1 was significantly decreased, the SOD activity increased significantly. It shows that SL extracts of PM2.5 infected RAW264.7 cell damage has obvious protective effect, the effect may be related to the direct protection of cells, reduce oxidative stress and inflammatory injury.
authors.forename: Xu-cen
authors.initials: XC
authors.lastname: Liu
authors.forename: Yu-jie
authors.initials: YJ
authors.lastname: Li
authors.forename: Ya-jie
authors.initials: YJ
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Xiao-gang
authors.initials: XG
authors.lastname: Weng
authors.lastname: Chen Ying
authors.forename: Wei-yan
authors.initials: WY
authors.lastname: Cai
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.forename: Xiao-xi
authors.initials: XX
authors.lastname: Kan
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: He-fei
authors.initials: HF
authors.lastname: Huang
authors.forename: Xiao-xin
authors.initials: XX
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D052638:Particulate Matter / Q000633:toxicity*; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D052638:Particulate Matter; D020011:Protective Agents; D017382:Reactive Oxygen Species