title: Urinary Leukotriene E4 Level in Non-Allergic Thai Young Children.
abstract: BACKGROUND
  Urinary leukotriene E4 (uLTE4) concentration represents body leukotriene synthesis. The level increases especially in respiratory allergic diseases. Researches of leukotriene production role in children with respiratory allergic diseases required the normal levels in non-allergic children as references.
  
  
  OBJECTIVE
  The study was to assess the reference values of uLTE4 in non-allergic Thai children measured by competitive enzyme-linked immunosorbent assay (ELISA) technique.
  
  
  METHODS
  Children who were admitted for elective surgery, aged 6 months to 5 years, were included in the study. Subjects who had acute illness, respiratory allergy (allergic rhinitis and asthma), and chronic diseases were excluded. Morning urine (5 ml) was collectedfor uLTE4 analysis by competitive ELISA technique.
  
  
  RESULTS
  There were 36 urinary samples from 36 subjects aged 6-60 months with 26 boys and 10 girls. The mean of uLTE4 concentration was 619.73 ± 701.32 pg/ml and the mean of uLTE4 concentration adjustedfor urinary creatinine concentrations was 1,328 ± 788.54 pg/mg creatinine. The mean of uLTE4 concentration in boys and girls were 1,349 ± 817.10 pg/mg creatinine and 1,275 ± 747.79 pg/mg creatinine respectively. The mean of uLTE4 concentration adjusted for urinary creatinine concentrations were 1,363 ± 886.65, 1,384 ± 771.81 and 1,223 ± 773.16 pg/mg creatinine for children with the age of 6-18, 19-36 and 37-60 months respectively.
  
  
  CONCLUSIONS
  The concentrations of uLTE4 and uLTE4 per creatinine were presented by age group in Thai very young children with non-respiratory disease. The concentrations were elevated in younger age group.
authors.forename: Phinidda
authors.initials: P
authors.lastname: Cha-umphol
authors.forename: Paskorn
authors.initials: P
authors.lastname: Sritipsukho
authors.forename: Sirikul
authors.initials: S
authors.lastname: Manochantr
authors.forename: Siripat
authors.initials: S
authors.lastname: Kiatpunsodsai
mesh_terms: D000367:Age Factors; D044466:Asian People; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D017999:Leukotriene E4 / Q000652:urine*; D008297:Male; D012016:Reference Values
chemical_list: D017999:Leukotriene E4