title: [Simultaneous Determination of Three Components in Ficus microcarpa Leaves by HPLC].
abstract: OBJECTIVE
  To develop an HPLC method for the content determination of benzoic acid, 4-hydroxyphenylacetic acid and acetosyringonecas in Ficus microcarpa Leaves.
  
  
  METHODS
  The determination was performed on Purospher® STAR C18 (250 mm x 4.6 mm,5 µm). The mobile phase was consisted of acetonitrile-0.2% phosphoric acid aqueous solution with linear gradient elution and the flow rate of 1.0 mL/min. The column temperature was set at 35 °C and the detection wavelength was 270 nm.
  
  
  RESULTS
  The linear range of benzoic acid, 4-hydroxyphenylacetic acid and acetosyringonecas was 0.0121-1.21 µg (r = 0.9995), 0.423-42.32 [Lg ( r = 0.9999) and 0.047-4.70 pg( r = 0. 9996) , respectively. The average recovery was 100.7, 101.2 and 96.5 respectively.
  
  
  CONCLUSIONS
  This method is simple,reproducible,and can be used for determination of three components of benzoic acid, 4-hydroxyphenylacetic acid and acetosyringonecas in Ficus microcarpa Leaves.
authors.forename: Tao-yang
authors.initials: TY
authors.lastname: Huang
authors.forename: Long-hao
authors.initials: LH
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Yan-jun
authors.initials: YJ
authors.lastname: Weng
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Xin-min
authors.initials: XM
authors.lastname: Xie
mesh_terms: D002851:Chromatography, High Pressure Liquid; D030681:Ficus / Q000737:chemistry*; D010648:Phenylacetates; D064209:Phytochemicals / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D010648:Phenylacetates; D064209:Phytochemicals; C008070:4-hydroxyphenylacetic acid