title: Asymmetric Bioconversion of Acetophenone in Nano-Sized Emulsion Using Rhizopus oryzae.
abstract: The fungal morphologies and pellet sizes were controlled in acetophenone reduction by Rhizopus oryzae. The acetophenone conversion and (S)-phenylethanol enantiomeric excesses (e.e.) reached the peak after 72 h of incubation when using pellets with 0.54 mm diameter, which showed an excellent performance compared with suspended mycelia, clumps, and pellets with 0.65 or 0.75 mm diameter. Furthermore, nano-sized acetophenone was used as a substrate to improve the performances of biotransformation work. The results showed that the conversion of nanometric acetophenone and (S)-phenylethanol e.e. reached the maximum (both >99%) after 32 h of incubation when using 0.54 mm diameter pellets, at least 24 h in advance of the control group. On the other hand, Tween 80 and 1, 2-propylene glycol showed low or no toxicity to cells. In conclusion, pellets and acetophenone nanoemulsions synergistically result in superior performances of acetophenone reduction.
authors.affiliation: College of Life Science and Agriculture, Zhoukou Normal University, Zhoukou, Henan 466001, P.R. China.
authors.forename: Qingzhi
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Life Science and Agriculture, Zhoukou Normal University, Zhoukou, Henan 466001, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shi
authors.affiliation: College of Life Science and Agriculture, Zhoukou Normal University, Zhoukou, Henan 466001, P.R. China.
authors.forename: Le
authors.initials: L
authors.lastname: He
authors.affiliation: College of Chemistry and Chemical Engineering, Zhoukou Normal University, Zhoukou, Henan 466001, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000098:Acetophenones / Q000737:chemistry / Q000378:metabolism*; D001711:Biotransformation; D005285:Fermentation; D012233:Rhizopus / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism
chemical_list: D000098:Acetophenones; C038699:acetophenone
keywords: Acetophenone; Bioreduction; Conversion; Enantiomeric excesses (e.e.); Nanoemulsion; Rhizopus oryzae