title: Increased expression of microRNA-191 as a potential serum biomarker for diagnosis and prognosis in human osteosarcoma.
abstract: BACKGROUND
  MicroRNA (miR)-191 has been observed to be overexpressed in osteosarcoma cell lines in comparison with osteoblasts.
  
  
  OBJECTIVE
  To investigate the clinical significance of miR-191 in human osteosarcomas.
  
  
  METHODS
  Quantitative PCR was performed to detect miR-191 expression in osteosarcoma tissues and patients' sera.
  
  
  RESULTS
  miR-191 expression levels, both in osteosarcoma tissues and patients' sera, were significantly higher than those in matched adjacent normal bone tissues and healthy controls (both P< 0.001). Importantly, miR-191 could efficiently screen osteosarcoma patients from healthy controls (Area under receiver operating characteristic curve, AUC = 0.808). Then, high serum miR-191 expression was significantly associated with advanced clinical stage (P = 0.001), large tumor size (P = 0.01) and positive distant metastasis (P = 0.001). Moreover, overall and disease-free survival durations in patients with high miR-191 expression were both shorter than those with low miR-191 expression. Multivariate analysis further identified serum miR-191 level as an independent and significant prognostic factor for both overall survival (P = 0.01) and disease-free survival (P = 0.02).
  
  
  CONCLUSIONS
  Our data provide new insights for the involvement of miR-191 in osteosarcoma and suggest that the increased expression of miR-191 may be associated with aggressive tumor progression and adverse outcome. Of note, serum miR-191 quantification may be a promising biomarker for the diagnosis and prognosis in osteosarcoma.
authors.affiliation: Department of Orthopedics, the Affiliated Hospital of Qinghai University, Xining, Qinghai, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Ji
authors.affiliation: Department of Orthopedics, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, China.
authors.forename: Zhitang
authors.initials: Z
authors.lastname: Dai
authors.affiliation: Department of Orthopedics, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Orthopedics, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, China.
authors.forename: Dongtang
authors.initials: D
authors.lastname: Yuan
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D012516:Osteosarcoma / Q000097:blood* / Q000473:pathology; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; C546177:MIRN191 microRNA, human; D035683:MicroRNAs
keywords: Osteosarcoma; diagnosis; microRNA-191; prognosis; real-time quantitative RT-PCR; serum