title: Molecular characterization of novel mucosotropic  papillomaviruses from a Florida manatee  (Trichechus manatus latirostris).
abstract: We isolated two new manatee papillomavirus (PV) types, TmPV3 and TmPV4, from a Florida manatee (Trichechus manatus latirostris). Two PV types were previously isolated from this species. TmPV1 is widely dispersed amongst manatees and a close-to-root PV; not much is known about TmPV2. The genomes of TmPV3 and TmPV4 were 7622 and 7771 bp in size, respectively. Both PVs had a genomic organization characteristic of all PVs, with one non-coding region and seven ORFs, including the E7 ORF that is absent in other cetacean PVs. Although these PVs were isolated from separate genital lesions of the same manatee, an enlarged E2/E4 ORF was found only in the TmPV4 genome. The full genome and L1 sequence similarities between TmPV3 and TmPV4 were 63.2 and 70.3 %, respectively. These genomes shared only 49.1 and 50.2 % similarity with TmPV1. The pairwise alignment of L1 nucleotide sequences indicated that the two new PVs nested in a monophyletic group of the genus Rhopapillomavirus, together with the cutaneotropic TmPV1 and TmPV2.
authors.affiliation: James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Maryam
authors.initials: M
authors.lastname: Zahin
authors.affiliation: James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Shin-Je
authors.initials: SJ
authors.lastname: Ghim
authors.affiliation: James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA.|Department of Biochemistry and Molecular Genetics, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Sujita
authors.initials: S
authors.lastname: Khanal
authors.affiliation: Georgia Aquarium, 225 Baker Street Northwest, Atlanta, GA 30313, USA.|Division of Comparative Pathology, Department of Pathology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
authors.forename: Gregory D
authors.initials: GD
authors.lastname: Bossart
authors.affiliation: James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Alfred B
authors.initials: AB
authors.lastname: Jenson
authors.affiliation: James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA.|Department of Medicine, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Joongho
authors.initials: J
authors.lastname: Joh
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D020457:Trichechus manatus / Q000821:virology*
chemical_list: D004279:DNA, Viral