title: Peroxidative metabolism of arachidonic acid in the course of Lyme arthritis.
abstract: OBJECTIVE
  The objective of the study was measurement of serum arachidonic acid level as well as the product of its peroxidation - 8-isoPGF2α, and the activity of phospholipase A2 and PAF-acetylhydrolase that participate in releasing 8-isoPGF2α from glycerol skeleton, and the potential designation of their role in the pathomechanism of Lyme disease (LD).
  
  
  METHODS
  Changes in the phospholipid arachidonic acid level and the level of 8-isoPGF2α were determined in the plasma and urine of patients with LA (n=57) and of healthy controls (n=41). The activity of phospholipase A2 and PAF acetylhydrolase were assayed. All examined parameters were also measured in the plasma of some LA patients (n=13) after antibiotics treatment.
  
  
  RESULTS
  An almost 3-fold higher level of the total plasma 8-isoPGF2α was observed in LA patients compared to the controls, while in the urine it increased over 5-fold. The plasma PLA2 activity was more than 3-fold higher in LA patients than in the healthy subjects, while PAF acetylhydrolase activity was observed to be modestly, but not significantly lower. The total 8-isoPGF2α level in the plasma and urine of LA patients was significantly lower after antibiotics treatment. The plasma activity of PAF-AH in the LA patients was increased, while the cPLA2 activity decreased after antibiotics treatment.
  
  
  CONCLUSIONS
  It may be suggested that in the course of LA, the level of binding 8-isoPGF2α is significantly enhanced, and it may also be suggested that uncontrolled changes in the lipid status of some patients may make their Lyme arthritis unresponsive to antibiotics.
authors.affiliation: Department of Analytical Chemistry, Medical University of Bialystok, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Łuczaj
authors.affiliation: Department of Infectious Diseases and Neuroinfection, Medical University of Bialystok, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Moniuszko
authors.affiliation: Department of Analytical Chemistry, Medical University of Bialystok, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Rusak
authors.affiliation: Department of Infectious Diseases and Neuroinfection, Medical University of Bialystok, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Zajkowska
authors.affiliation: Department of Infectious Diseases and Neuroinfection, Medical University of Bialystok, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Pancewicz
authors.affiliation: Department of Analytical Chemistry, Medical University of Bialystok, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Skrzydlewska
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000378:metabolism; D000328:Adult; D000368:Aged; D016718:Arachidonic Acid / Q000097:blood*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000652:urine; D005260:Female; D006801:Humans; D008193:Lyme Disease / Q000097:blood / Q000378:metabolism* / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D054467:Phospholipases A2 / Q000378:metabolism*; D011044:Poland; D055815:Young Adult
chemical_list: C075750:8-epi-prostaglandin F2alpha; D016718:Arachidonic Acid; D015237:Dinoprost; D054467:Phospholipases A2; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase