title: CD10, BCL6, and MUM1 expression in diffuse large B-cell lymphoma on FNA samples.
abstract: BACKGROUND
  Gene expression profiling has divided diffuse large B-cell lymphoma (DLBCL) into 2 main subgroups: germinal center B (GCB) and non-GCB type. This classification is reproducible by immunohistochemistry using specific antibodies such as CD10, B-cell lymphoma 6 (BCL6), and multiple myeloma oncogene 1 (MUM1). Fine-needle aspiration (FNA) plays an important role in the diagnosis of non-Hodgkin lymphoma, and in some cases FNA may be the only available pathological specimen. The objectives of the current study were to evaluate CD10, BCL6, and MUM1 immunostaining on FNA samples by testing the CD10, BCL6, and MUM1 algorithm on both FNA cell blocks (CB) and conventional smears (CS), evaluating differences in CB and CS immunocytochemical (ICC) performance, and comparing results with histological data.
  
  
  METHODS
  Thirty-eight consecutive DLBCL cases diagnosed by FNA were studied. Additional passes were used to prepare CB in 22 cases and CS in 16 cases; the corresponding sections and smears were immunostained using CD10, BCL6, and MUM1 in all cases. The data obtained were compared with histological immunostaining in 24 cases.
  
  
  RESULTS
  ICC was successful in 33 cases (18 CB and 15 CS) and not evaluable in 5 cases (4 CB and 1 CS). The CD10-BCL6-MUM1 algorithm subclassified DLBCL as GCB (9 cases) and non-GCB (24 cases). ICC data were confirmed on histologic staining in 24 cases.
  
  
  CONCLUSIONS
  CD10, BCL6, and MUM1 ICC staining can be performed on FNA samples. The results herein prove it is reliable both on CB and CS, and is equally effective and comparable to immunohistochemistry data.
authors.affiliation: Department of Public Health, University of Naples "Federico II, " Naples, Italy.
authors.forename: Immacolata
authors.initials: I
authors.lastname: Cozzolino
authors.affiliation: Department of Advanced Biomedical Sciences, University of Naples "Federico II, " Naples, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Varone
authors.affiliation: Department of Medicine and Surgery, University of Naples "Federico II, " Naples, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Picardi
authors.affiliation: Department of Medicine and Surgery, University of Salerno, Salerno, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Baldi
authors.affiliation: Department of Medicine and Surgery, University of Salerno, Salerno, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Memoli
authors.affiliation: Department of Advanced Biomedical Sciences, University of Naples "Federico II, " Naples, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ciancia
authors.affiliation: Department of Medicine and Surgery, University of Salerno, Salerno, Italy.
authors.forename: Carmine
authors.initials: C
authors.lastname: Selleri
authors.affiliation: Department of Advanced Biomedical Sciences, University of Naples "Federico II, " Naples, Italy.
authors.forename: Gaetano
authors.initials: G
authors.lastname: De Rosa
authors.affiliation: Department of Public Health, University of Naples "Federico II, " Naples, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Vetrani
authors.affiliation: Department of Medicine and Surgery, University of Salerno, Salerno, Italy.
authors.forename: Pio
authors.initials: P
authors.lastname: Zeppa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044963:Biopsy, Fine-Needle; D015331:Cohort Studies; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D050835:Interferon Regulatory Factors / Q000235:genetics*; D016014:Linear Models; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D015260:Neprilysin / Q000235:genetics*; D011379:Prognosis; D011446:Prospective Studies; D051560:Proto-Oncogene Proteins c-bcl-6; D000099218:Interferon Regulatory Factor-4
chemical_list: D004268:DNA-Binding Proteins; D050835:Interferon Regulatory Factors; D015260:Neprilysin; D051560:Proto-Oncogene Proteins c-bcl-6; D000099218:Interferon Regulatory Factor-4; C084103:BCL6 protein, human
keywords: B-cell lymphoma 6 (BCL6); CD10; cell block; diffuse large B-cell lymphoma (DLBCL); fine-needle aspiration (FNA); multiple myeloma oncogene 1 (MUM1); non-Hodgkin lymphoma