title: PGBL inhibits the RAW 264.7 cells to express inflammatory factor.
abstract: Polysaccharides derived from Ginkgo biloba leaf (PGBL) is a kind of active ingredient came out from ginkgo biloba leaf extractions. Previous studies have shown that PGBL has a good anti-inflammatory effect. However, the mechanism is not clear. This study is to investigate the modulated immunity effect of PGBL on RAW264.7 cells. Here we showed that lipopolysaccharide (LPS) induces the expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and this induction can be repressed by PGBL treatment both in protein level and mRNA level, and PGBL strongly reduced the translocation of nuclear factor (NF)-κB to the cell nucleus. These findings demonstrate that PGBL can decrease the sensitivity of monocytes to LPS, and PGBL has applications in systemic inflammation and immune diseases.
authors.affiliation: Department of Cell Biology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Linghui
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department Of Ophthalmology, Second Hospital, Jilin University, Changchun 130041, P.R. China.
authors.forename: Guirong
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Cell Biology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Jianan
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Cell Biology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Cell Biology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Fei
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D020441:Ginkgo biloba / Q000737:chemistry*; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D016328:NF-kappa B / Q000276:immunology; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D000067996:RAW 264.7 Cells; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha
keywords: IL-6; LPS; NF–κB; PGBL; TNF-α