title: Haploid Strategies for Functional Validation of Plant Genes.
abstract: Increasing knowledge of plant genome sequences requires the development of more reliable and efficient genetic approaches for genotype-phenotype validation. Functional identification of plant genes is generally achieved by a combination of creating genetic modifications and observing the according phenotype, which begins with forward-genetic methods represented by random physical and chemical mutagenesis and move towards reverse-genetic tools as targeted genome editing. A major bottleneck is time need to produce modified homozygous genotypes that can actually be used for phenotypic validation. Herein, we comprehensively address and compare available experimental approaches for functional validation of plant genes, and propose haploid strategies to reduce the time needed and cost consumed for establishing gene function.
authors.affiliation: Maize Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, China; Department of Agronomy, Iowa State University, 100 Osborn Drive, Ames, IA 50011, USA. Electronic address: shenyaou@gmail.com.
authors.forename: Yaou
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Maize Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, China.
authors.forename: Guangtang
authors.initials: G
authors.lastname: Pan
authors.affiliation: Department of Agronomy, Iowa State University, 100 Osborn Drive, Ames, IA 50011, USA. Electronic address: thomasl@iastate.edu.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lübberstedt
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics*; D004251:DNA Transposable Elements; D063990:Gene Ontology; D017343:Genes, Plant; D005818:Genetic Engineering; D018745:Genome, Plant; D005838:Genotype; D006238:Haploidy; D058977:Molecular Sequence Annotation; D016296:Mutagenesis; D010641:Phenotype; D030821:Plants, Genetically Modified; D059386:Reverse Genetics; D014170:Transformation, Genetic
chemical_list: D004251:DNA Transposable Elements
keywords: functional validation; genetic approaches; haploid mutagenesis; haploid transformation; plant genes