title: Study of the radiation effect of 99Mo/99mTc generator on Bacillus subtilis and Bacillus pumilus species.
abstract: In this work, molybdenum-99 loaded columns were challenged with Bacillus subtilis vegetative cells and Bacillus pumilus spores inside and outside the alumina column, and microbial recovery and radiation effect were assessed. Alumina was a barrier for the passage of microorganisms regardless the species, whilst spores were more retained than vegetative cells with a lower microbial recovery, without significant differences between 9.25 and 74 GBq generators. Bacillus pumilus biological indicator showed lower recoveries, suggesting a radiation inactivating effect on microorganisms.
authors.affiliation: Radiopharmacy Center-Nuclear Energy and Research Institute (IPEN-CNEN/SP), Avenue Professor Lineu Prestes 2242, Butantã, CEP 05508-000 São Paulo, SP, Brazil. Electronic address: ntfukumo@ipen.br.
authors.forename: Neuza T O
authors.initials: NTO
authors.lastname: Fukumori
authors.affiliation: Radiopharmacy Center-Nuclear Energy and Research Institute (IPEN-CNEN/SP), Avenue Professor Lineu Prestes 2242, Butantã, CEP 05508-000 São Paulo, SP, Brazil.
authors.forename: Erica M M
authors.initials: EMM
authors.lastname: Endo
authors.affiliation: Radiopharmacy Center-Nuclear Energy and Research Institute (IPEN-CNEN/SP), Avenue Professor Lineu Prestes 2242, Butantã, CEP 05508-000 São Paulo, SP, Brazil.
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Felgueiras
authors.affiliation: Radiopharmacy Center-Nuclear Energy and Research Institute (IPEN-CNEN/SP), Avenue Professor Lineu Prestes 2242, Butantã, CEP 05508-000 São Paulo, SP, Brazil.
authors.forename: Margareth M N
authors.initials: MMN
authors.lastname: Matsuda
authors.affiliation: Radiopharmacy Center-Nuclear Energy and Research Institute (IPEN-CNEN/SP), Avenue Professor Lineu Prestes 2242, Butantã, CEP 05508-000 São Paulo, SP, Brazil.
authors.forename: João A
authors.initials: JA
authors.lastname: Osso Junior
mesh_terms: D000537:Aluminum Oxide; D001407:Bacillus / Q000528:radiation effects*; D001412:Bacillus subtilis / Q000528:radiation effects*; D058491:Bacterial Load / Q000379:methods; D006801:Humans; D008982:Molybdenum / Q000008:administration & dosage*; D011829:Radiation Dosage; D011868:Radioisotopes / Q000008:administration & dosage*; D019275:Radiopharmaceuticals / Q000008:administration & dosage*; D013053:Spectrophotometry; D013171:Spores, Bacterial / Q000528:radiation effects; D013667:Technetium / Q000008:administration & dosage*
chemical_list: D011868:Radioisotopes; D019275:Radiopharmaceuticals; D013667:Technetium; D008982:Molybdenum; D000537:Aluminum Oxide
keywords: (99)Mo/(99m)Tc generator; Ionizing radiation effect; Microbial recovery; Microorganisms; Sterilization