title: Dysregulated miR-645 affects the proliferation and invasion of head and neck cancer cell.
abstract: OBJECTIVE
  Dysregulated miRNAs play an important role in many malignant tumors. However, elucidating the roles of miRNAs in cancer biology, especially in epithelial cancers, remains an ongoing process. In this study, we identified the differentially expressed miR-645 in the progressing of head and neck squamous cell carcinoma (HNSCC) and investigated its biological function.
  
  
  METHODS
  The association between clinicopathological parameters and the expression levels of the candidated miRNAs were analyzed by using the Kaplan-Meier survival analysis. The cell growth, invasion and migration potential, and clone formation were observed to detect the functions of the miRNAs in HNSCC cells.
  
  
  RESULTS
  In the 34 HNSCC tissues with lymph node metastasis, the expression level of miR-645 was 0.54 ± 0.12, and the expression level was 0.22 ± 0.05 in the 28 tissues with non lymph node metastasis (p = 0.017). In patients with HNSCC, higher level of miR-645 expression significantly correlates with worse overall survival (p = 0.04). Ectopic expression of miR-645 promoted cell invasion and migration.
  
  
  CONCLUSIONS
  miR-645 play a key role in cell invasion and metastasis and their expression correlates with overall survival in the patients with HNSCC.
authors.affiliation: Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, No. 1, East Jian she Road, Zhengzhou, 450052 Henan China.|Institute of Clinical Medicine, The First Affiliated Hospital of Zhengzhou University, No. 1, East Jian she Road, Zhengzhou, 450052 Henan China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, No. 1, East Jian she Road, Zhengzhou, 450052 Henan China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Neuroimmunology Research, The Henan Academy of Medical and Pharmacologic Sciences, Zheng-Zhou University, Daxue Rd No. 40, Zhengzhou, 450052 Henan China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011 China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.affiliation: Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, No. 1, East Jian she Road, Zhengzhou, 450052 Henan China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, No. 1, East Jian she Road, Zhengzhou, 450052 Henan China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, No. 1, East Jian she Road, Zhengzhou, 450052 Henan China.
authors.forename: Junfang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, No. 1, East Jian she Road, Zhengzhou, 450052 Henan China.
authors.forename: Minglei
authors.initials: M
authors.lastname: Sun
authors.affiliation: Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, No. 1, East Jian she Road, Zhengzhou, 450052 Henan China.
authors.forename: Xinguang
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011 China.
authors.forename: Wantao
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, No. 1, East Jian she Road, Zhengzhou, 450052 Henan China.
authors.forename: Xinming
authors.initials: X
authors.lastname: Li
keywords: Growth; HNSCC; Invasion; Metastasis; miR-645