title: Preventive Activity against Influenza (H1N1) Virus by Intranasally Delivered RNA-Hydrolyzing Antibody in Respiratory Epithelial Cells of Mice.
abstract: The antiviral effect of a catalytic RNA-hydrolyzing antibody, 3D8 scFv, for intranasal administration against avian influenza virus (H1N1) was described. The recombinant 3D8 scFv protein prevented BALB/c mice against H1N1 influenza virus infection by degradation of the viral RNA genome through its intrinsic RNA-hydrolyzing activity. Intranasal administration of 3D8 scFv (50 μg/day) for five days prior to infection demonstrated an antiviral activity (70% survival) against H1N1 infection. The antiviral ability of 3D8 scFv to penetrate into epithelial cells from bronchial cavity via the respiratory mucosal layer was confirmed by immunohistochemistry, qRT-PCR, and histopathological examination. The antiviral activity of 3D8 scFv against H1N1 virus infection was not due to host immune cytokines or chemokines, but rather to direct antiviral RNA-hydrolyzing activity of 3D8 scFv against the viral RNA genome. Taken together, our results suggest that the RNase activity of 3D8 scFv, coupled with its ability to penetrate epithelial cells through the respiratory mucosal layer, directly prevents H1N1 virus infection in a mouse model system.
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon 16419, Korea. seungchan1007@gmail.com.
authors.forename: Seungchan
authors.initials: S
authors.lastname: Cho
authors.affiliation: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 120, Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. yellow0891@daum.net.
authors.forename: Ha-Na
authors.initials: HN
authors.lastname: Youn
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon 16419, Korea. hmphuong2802@gmail.com.
authors.forename: Phuong Mai
authors.initials: PM
authors.lastname: Hoang
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon 16419, Korea. sungle89@gmail.com.
authors.forename: Sungrae
authors.initials: S
authors.lastname: Cho
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon 16419, Korea. happykke6@naver.com.
authors.forename: Kee-Eun
authors.initials: KE
authors.lastname: Kim
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon 16419, Korea. meitantei007@naver.com.
authors.forename: Eui-Joon
authors.initials: EJ
authors.lastname: Kil
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon 16419, Korea. asteroid1975@gmail.com.
authors.forename: Gunsup
authors.initials: G
authors.lastname: Lee
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon 16419, Korea. munju2004@naver.com.
authors.forename: Mun-Ju
authors.initials: MJ
authors.lastname: Cho
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon 16419, Korea. rsef741@gmail.com.
authors.forename: Juhyun
authors.initials: J
authors.lastname: Hong
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500, Kongjwipatjwi-ro, Iseomyeon, Wanju 55365, Korea. pcs1778@korea.kr.
authors.forename: Sung-June
authors.initials: SJ
authors.lastname: Byun
authors.affiliation: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 120, Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. yellow0891@daum.net.
authors.forename: Chang-Seon
authors.initials: CS
authors.lastname: Song
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon 16419, Korea. cell4u@skku.edu.
authors.forename: Sukchan
authors.initials: S
authors.lastname: Lee
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D017151:Antibodies, Catalytic / Q000008:administration & dosage*; D000998:Antiviral Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004847:Epithelial Cells / Q000276:immunology*; D006868:Hydrolysis; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D012367:RNA, Viral / Q000378:metabolism; D012260:Ribonucleases / Q000008:administration & dosage*; D057127:Single-Chain Antibodies / Q000008:administration & dosage* / Q000493:pharmacokinetics; D016896:Treatment Outcome
chemical_list: D017151:Antibodies, Catalytic; D000998:Antiviral Agents; D012367:RNA, Viral; D057127:Single-Chain Antibodies; D012260:Ribonucleases
keywords: 3D8 scFv; antiviral effect; influenza virus; intranasal administration; nuclease activity; respiratory mucosal layer