title: Short communication: Distribution of Porphyromonas gulae fimA genotypes in oral specimens from dogs with mitral regurgitation.
abstract: Porphyromonas gulae, a suspected pathogen for periodontal disease in dogs, possesses approximately 41-kDa fimbriae (FimA) that are encoded by the fimA gene. In the present study, the association of fimA genotypes with mitral regurgitation (MR) was investigated. Twenty-five dogs diagnosed with MR (age range 6-13 years old, average 10.8 years) and 32 healthy dogs (8-15 years old, average 10.8 years) were selected at the participating clinics in a consecutive manner during the same time period. Oral swab specimens were collected from the dogs and bacterial DNA was extracted, then polymerase chain reaction analysis was performed using primers specific for each fimA genotype, with the dominant genotype determined. The rate for genotype C dominant specimens was 48.0% in the MR group, which was significantly higher than that in the control group (18.8%) (P <0.05). These results suggest that P. gulae fimA genotype C is associated with MR.
authors.affiliation: Department of Pharmacology, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252-5201, Japan. Electronic address: shirai@azabu-u.ac.jp.
authors.forename: Mitsuyuki
authors.initials: M
authors.lastname: Shirai
authors.affiliation: Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan. Electronic address: rnomura@dent.osaka-u.ac.jp.
authors.forename: Ryota
authors.initials: R
authors.lastname: Nomura
authors.affiliation: Department of Veterinary Public Health II, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252-5201, Japan. Electronic address: katohy@azabu-u.ac.jp.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kato
authors.affiliation: Department of Molecular Biology, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252-5201, Japan. Electronic address: murakami@azabu-u.ac.jp.
authors.forename: Masaru
authors.initials: M
authors.lastname: Murakami
authors.affiliation: Department of Pharmacology, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252-5201, Japan. Electronic address: v09058@azabu-u.ac.jp.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Kondo
authors.affiliation: Department of Pharmacology, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252-5201, Japan. Electronic address: v09083@azabu-u.ac.jp.
authors.forename: Soraaki
authors.initials: S
authors.lastname: Takahashi
authors.affiliation: Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8556, Japan. Electronic address: yoca777@live.jp.
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Yamasaki
authors.affiliation: Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8556, Japan. Electronic address: mnakano@cc.okayama-u.ac.jp.
authors.forename: Michiyo
authors.initials: M
authors.lastname: Matsumoto-Nakano
authors.affiliation: Yasuda Veterinary Clinic, Meguro, Tokyo 152-0034, Japan. Electronic address: narai@slj.co.jp.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Arai
authors.affiliation: Yasuda Veterinary Clinic, Meguro, Tokyo 152-0034, Japan. Electronic address: hidemi@yasuda-vet.org.
authors.forename: Hidemi
authors.initials: H
authors.lastname: Yasuda
authors.affiliation: Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan. Electronic address: nakano@dent.osaka-u.ac.jp.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakano
authors.affiliation: Department of Pharmacology, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252-5201, Japan. Electronic address: asai@azabu-u.ac.jp.
authors.forename: Fumitoshi
authors.initials: F
authors.lastname: Asai
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D004285:Dogs; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005838:Genotype; D008944:Mitral Valve Insufficiency / Q000382:microbiology / Q000662:veterinary*; D017861:Porphyromonas / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D036781:Fimbriae Proteins
keywords: Porphyromonas gulae; fimbriae; mitral regurgitation; periodontal disease