title: Electrical sensing of DNA-hybridization using two-port network based on suspended carbon nanotube membrane.
abstract: An approach was presented for electrical sensing of deoxyribonucleic acid (DNA)-hybridization in solution using a two-port network based on single-walled suspended carbon nanotube (SWCNT) membrane. A single stranded probe DNA (5'-NH2-(CH2)6-CGC CGA TTG GAC AAA ACT TAA A-3') was immobilized on the SWCNT membrane. A solution with the complementary single stranded DNA (D'FITC: 5'-FITC-T TTA AGT TTT GTC CAA TCG GCG-3') in various concentrations was then dropped on the membrane. The two-port network composed of the suspended SWCNT membrane and its underneath gate were characterized by coupling with a vector network analyzer. The resonance frequency of transmission coefficient S21 was observed to be around 10 MHz. The resonance frequency shifts with DNA-hybridization, and the sensing limit was approximately 50 nM. The advantages of this approach include low-noise frequency output, solution based real time detection and capable of on-chip integration, etc.
authors.affiliation: Pen-Tung Sah Institute of Micro-Nano Science & Technology, Xiamen University, Xiamen, 361005, People's Republic of China.
authors.forename: Tun
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Physics, Xiamen University, Xiamen, 361005, China.
authors.forename: Ze-Zhi
authors.initials: ZZ
authors.lastname: Zheng
authors.affiliation: Pen-Tung Sah Institute of Micro-Nano Science & Technology, Xiamen University, Xiamen, 361005, People's Republic of China.
authors.forename: Xing-Xing
authors.initials: XX
authors.lastname: Feng
authors.affiliation: Pen-Tung Sah Institute of Micro-Nano Science & Technology, Xiamen University, Xiamen, 361005, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Pen-Tung Sah Institute of Micro-Nano Science & Technology, Xiamen University, Xiamen, 361005, People's Republic of China. lm@xmu.edu.cn.
authors.forename: Miao
authors.initials: M
authors.lastname: Lu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*
chemical_list: D037742:Nanotubes, Carbon
keywords: DNA detection; Suspended carbon nanotube membrane; Two-port network