title: Elevation of miR-27b by HPV16 E7 inhibits PPARγ expression and promotes proliferation and invasion in cervical carcinoma cells.
abstract: MicroRNAs (miRNAs) have been reported to be involved in multiple biological pathways that can influence tumor progression and metastasis. High-risk human papillomavirus (HR-HPVs) is aetiologically correlated to cervical cancer. Recently, miRNAs were reported to be regulated by virus and play pivotal roles in HPV-related tumor progression. However, the underlying mechanism remains poorly understood. In the present study, we report that HPV16 E7 upregulated miR-27b to promote proliferation and invasion in cervical cancer. The results showed that PPARγ, as a target of miR-27b, played a significant role in suppressing cervical cancer progression by downregulating the sodium-hydrogen exchanger isoform 1 (NHE1). It was also shown that the inhibition of miR-27b diminished the ability of HPV16 E7 to suppress PPARγ or activate NHE1 expression. In addition, we observed high expression of miR-27b and NHE1, but low expression of PPARγ in HPV16-positive cervical cancer tissues. In summary, the present study revealed that miR-27b is upregulated by HPV16 E7 to inhibit PPARγ expression and promotes proliferation and invasion in cervical carcinoma cells.
authors.affiliation: Department of Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China.
authors.forename: Shimeng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China.
authors.forename: Xinru
authors.initials: X
authors.lastname: Mao
authors.affiliation: Department of Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China.
authors.forename: Jinlan
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China.
authors.forename: Junyao
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China.
authors.forename: Xiaomao
authors.initials: X
authors.lastname: Yin
authors.affiliation: Department of Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
mesh_terms: D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D027682:Cation Transport Proteins / Q000096:biosynthesis / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000235:genetics; D047495:PPAR gamma / Q000096:biosynthesis / Q000235:genetics*; D050725:Papillomavirus E7 Proteins / Q000378:metabolism; D030361:Papillomavirus Infections / Q000235:genetics / Q000473:pathology / Q000821:virology; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers / Q000096:biosynthesis / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology
chemical_list: D027682:Cation Transport Proteins; C531388:MIRN27 microRNA, human; D035683:MicroRNAs; D047495:PPAR gamma; D050725:Papillomavirus E7 Proteins; C490998:SLC9A1 protein, human; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers; C059731:oncogene protein E7, Human papillomavirus type 16