title: Ultraviolet light-emitting-diode irradiation inhibits TNF-α and IFN-γ-induced expression of ICAM-1 and STAT1 phosphorylation in human keratinocytes.
abstract: OBJECTIVE
  Ultraviolet light-emitting diodes (UV-LEDs) are a novel light source for phototherapy. This research investigated the in vitro safety and efficacy of UV-LEDs as a phototherapeutic device for atopic dermatitis (AD).
  
  
  METHODS
  Human keratinocytes and fibroblasts were irradiated by UV-LEDs with a center wavelength of 310 and 340 nm. We examined the effects of UV-LED irradiation on the suppression of TNF-α/IFN-γ-induced activation of STAT1 and ICAM-1 and on NF-κB expression; we used the following methods: cell viability assay, reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, Western blotting, and immunocytochemistry.
  
  
  RESULTS
  We observed anti-inflammatory responses through the suppression of TNF-α/IFN-γ-induced expression of TARC and MCP-1/CCL2, IL-1beta, IL-6, and sICAM-1 via blockage of ICAM-1 activation and subsequent activation of STAT1 and NF-κB. The results suggested that UV-LED irradiation inhibited ICAM expression by suppressing TNF-α/IFN-γ-induced NF-κB activation in vitro.
  
  
  CONCLUSIONS
  We concluded that novel UV-LED (310 and 340 nm) modalities were effective for the treatment of AD and may be promising for the treatment of inflammatory skin diseases.
authors.affiliation: Department of Medicine, Graduate, School, Chung-Ang, University, Seoul, Korea.|Department of Dermatology, Chung-Ang, University, College of Medicine, Seoul, Korea.
authors.forename: Tae-Rin
authors.initials: TR
authors.lastname: Kwon
authors.affiliation: Department of Medicine, Graduate, School, Chung-Ang, University, Seoul, Korea.|Department of Dermatology, Chung-Ang, University, College of Medicine, Seoul, Korea.
authors.forename: Chang Taek
authors.initials: CT
authors.lastname: Oh
authors.affiliation: Department of Dermatology, Chung-Ang, University, College of Medicine, Seoul, Korea.
authors.forename: Eun Ja
authors.initials: EJ
authors.lastname: Choi
authors.affiliation: Department of Dermatology, Chung-Ang, University, College of Medicine, Seoul, Korea.
authors.forename: Soon Re
authors.initials: SR
authors.lastname: Kim
authors.affiliation: Department of Dermatology, Chung-Ang, University, College of Medicine, Seoul, Korea.
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Jang
authors.affiliation: Department of Dermatology, Chung-Ang, University, College of Medicine, Seoul, Korea.
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Ko
authors.affiliation: Department of Materials Science and Engineering, Seoul National University, Seoul, Korea.
authors.forename: Daewoong
authors.initials: D
authors.lastname: Suh
authors.affiliation: Department of Dermatology, College of Medicine, Catholic Kwandong University, International St.Mary's Hospital, Incheon, Korea.
authors.forename: Kwang Ho
authors.initials: KH
authors.lastname: Yoo
authors.affiliation: Department of Medicine, Graduate, School, Chung-Ang, University, Seoul, Korea.|Department of Dermatology, Chung-Ang, University, College of Medicine, Seoul, Korea.
authors.forename: Beom Joon
authors.initials: BJ
authors.lastname: Kim
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007371:Interferon-gamma / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism / Q000528:radiation effects*; D010766:Phosphorylation / Q000528:radiation effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050794:STAT1 Transcription Factor / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014466:Ultraviolet Rays; D014467:Ultraviolet Therapy / Q000295:instrumentation
chemical_list: D015415:Biomarkers; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma
keywords: HaCaT; UV-LEDs; atopic dermatitis