title: Toxicity detection using lysosomal enzymes, glycoamylase and thioredoxin fused with fluorescent protein in Saccharomyces cerevisiae.
abstract: Saccharomyces cerevisiae is the simplest and a favorite eukaryotic system that contains lysosome and thus, is a suitable organism for monitoring some toxic effects in environmental pollution. In this study, S. cerevisiae was transformed with two recombinant plasmids. Sporulation-specific glycoamylase (SGA1), which was upregulated in response to arsenic, was fused with the blue fluorescent protein (BFP) for the construction of an oxidative stress-causing chemicals sensor. Additionally, thioredoxin (TRX2), a protein overexpressed exclusively under tetracycline's influence, fused with the cyan fluorescent protein (CFP) to create a detector for this kind of chemical. In summary, we developed two recombinant S. cerevisiae that facilitate the detection of both kinds of toxic chemicals, specifically visualized by different color indicators.
authors.affiliation: Department of Bioprocess Engineering, Chonbuk National University, 664-14 Duckjin-dong, Jeonju 561-756, South Korea.
authors.forename: Ngoc-Tu
authors.initials: NT
authors.lastname: Nguyen
authors.affiliation: Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University(,) 664-14 Duckjin-dong, Jeonju 561-756, South Korea.
authors.forename: Hwa-Yoon
authors.initials: HY
authors.lastname: Shin
authors.affiliation: Department of Microbiology, Chungbuk National University, 410 Sungbong-Ro, Heungduk-Gu, Cheongju 361-763, South Korea. Electronic address: kyh@chungbuk.ac.kr.
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Bioprocess Engineering, Chonbuk National University, 664-14 Duckjin-dong, Jeonju 561-756, South Korea; Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University(,) 664-14 Duckjin-dong, Jeonju 561-756, South Korea. Electronic address: jihomin@jbnu.ac.kr.
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
mesh_terms: D000818:Animals; D001241:Aspirin / Q000633:toxicity; D015374:Biosensing Techniques / Q000379:methods*; D003621:Daphnia / Q000187:drug effects; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology; D019216:Metals, Heavy / Q000633:toxicity; D010575:Pesticides / Q000633:toxicity; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013752:Tetracycline / Q000633:toxicity; D013879:Thioredoxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018675:Toxicity Tests / Q000379:methods*
chemical_list: C485182:Cyan Fluorescent Protein; D008164:Luminescent Proteins; D019216:Metals, Heavy; D010575:Pesticides; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C409504:blue fluorescent protein, Aequorea victoria; D049452:Green Fluorescent Proteins; D013879:Thioredoxins; D005087:Glucan 1,4-alpha-Glucosidase; C109934:SGA1 protein, S cerevisiae; D013752:Tetracycline; D001241:Aspirin
keywords: Fluorescent proteins; Glycoamylase; Heavy metals; Lysosomal proteins; Pharmaceuticals; Thioredoxin