title: Tritrichomonas foetus infection in cat - first detection in Poland.
abstract: Tritrichomonas foetus, a parasite of cattle reproductive system, has been recently discovered as a cause of disease in cats in many countries. T. foetus infects and colonizes cat's ileum, caecum, colon and can lead to enteritis. This paper presents the first clinical case of cat intestinal trichomonosis caused by T. foetus in Poland. The material for this study was a smear collected from a 6-month-old male British Shorthair cat. The presence of parasitic protozoan was determined via microscopic examination and confirmed by amplification of T. foetus rDNA using polymerase chain reaction (PCR) technique. In the first PCR reaction, a DNA of Trichomonadidae was identified and in the second PCR, T. foetus was detected. The T. foetus positive products from the second PCR reaction were sequenced. Interpretation of the sequencing results of obtained amplicons by comparing them with the GenBank database proved that the causative agent, in this case, was T. foetus.
authors.forename: Joanna
authors.initials: J
authors.lastname: Dąbrowska
authors.forename: Jacek
authors.initials: J
authors.lastname: Karamon
authors.forename: Maciej
authors.initials: M
authors.lastname: Kochanowski
authors.forename: Roman
authors.initials: R
authors.lastname: Jędryczko
authors.forename: Tomasz
authors.initials: T
authors.lastname: Cencek
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000469:parasitology* / Q000473:pathology; D002415:Cats; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003967:Diarrhea / Q000469:parasitology / Q000662:veterinary; D005243:Feces / Q000469:parasitology; D008853:Microscopy; D008969:Molecular Sequence Data; D011044:Poland; D016133:Polymerase Chain Reaction; D011529:Protozoan Infections, Animal / Q000469:parasitology* / Q000473:pathology; D017422:Sequence Analysis, DNA; D016835:Tritrichomonas foetus / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal