title: Cytotoxic T cell responses are enhanced by antigen design involving the presentation of MUC1 peptide on cholera toxin B subunit.
abstract: Induction of cytotoxic T lymphocytes (CTL) is critical to cancer vaccine based immunotherapy. Efforts to elicit CTLs against tumor MUC1 with peptide based vaccine have not been successful in clinical application. We have design a MUC1 vaccine by replacing B cell epitope of CTB with MUC1 VNTR peptide. Immunization with hybrid CTB-MUC1 plus aluminum hydroxide and CpG adujuvant (CTB-MUC1-Alum-CpG) induce MUC1-specific CTLs in mice. Moreover, this vaccination can prevent tumor growth and reduce tumor burden in MUC1+B16 mice model. Meanwhile, CTB-MUC1-Alum-CpG vaccination can promote Th1 cells and CD8+ T cells inflate to tumor tissue. Our approach might be applicable to other cancer vaccine design.
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.|Laboratory of Cellular and Molecular Biology, Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China.|Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Wuguang
authors.initials: W
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Lingchong
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Laboratory of Cellular and Molecular Biology, Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China.|Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Zhanpeng
authors.initials: Z
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Laboratory of Cellular and Molecular Biology, Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China.|Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Cao
authors.affiliation: Laboratory of Cellular and Molecular Biology, Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China.|Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Hu
authors.affiliation: Laboratory of Cellular and Molecular Biology, Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China.|Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Medical Science and Department of Pharmacology and Physiology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Medical Science and Department of Pharmacology and Physiology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Institute of Medical Science and Department of Pharmacology and Physiology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Laboratory of Cellular and Molecular Biology, Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China.|Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.|Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Rongxiu
authors.initials: R
authors.lastname: Li
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D015153:Blotting, Western; D019496:Cancer Vaccines / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology*; D004195:Disease Models, Animal; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018598:Minisatellite Repeats / Q000276:immunology; D018396:Mucin-1 / Q000276:immunology*; D016133:Polymerase Chain Reaction; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D019496:Cancer Vaccines; D018985:Epitopes, B-Lymphocyte; D018396:Mucin-1; D022223:Vaccines, Subunit; D002772:Cholera Toxin
keywords: MUC1; antigen design; cytotoxic T cell response; immunotherapy; vaccine