title: Detection of N-(1-deoxy-D-fructos-1-yl) Fumonisins B₂ and B₃ in Corn by High-Resolution LC-Orbitrap MS.
abstract: The existence of glucose conjugates of fumonisin B₂ (FB₂) and fumonisin B₃ (FB₃) in corn powder was confirmed for the first time. These "bound-fumonisins" (FB₂ and FB₃ bound to glucose) were identified as N-(1-deoxy-D-fructos-1-yl) fumonisin B₂ (NDfrc-FB₂) and N-(1-deoxy-D-fructos-1-yl) fumonisin B₃ (NDfrc-FB₃) respectively, based on the accurate mass measurements of characteristic ions and fragmentation patterns using high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) analysis. Treatment on NDfrc-FB₂ and NDfrc-FB₃ with the o-phthalaldehyde (OPA) reagent also supported that D-glucose binding to FB₂ and FB₃ molecules occurred to their primary amine residues.
authors.affiliation: National Agriculture and Food Research Organization (NARO), National Food Research Institute, 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan. ymatuo@affrc.go.jp.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Matsuo
authors.affiliation: Thermo Fisher Scientific, C-2F, 3-9 Moriya-cho, Yokohama-shi, Kanagawa 221-0022, Japan. kentaro.takahara@thermofisher.com.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Takahara
authors.affiliation: National Agriculture and Food Research Organization (NARO), National Food Research Institute, 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan. sydney1219@nifty.com.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sago
authors.affiliation: National Agriculture and Food Research Organization (NARO), National Food Research Institute, 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan. kushirom@affrc.go.jp.
authors.forename: Masayo
authors.initials: M
authors.lastname: Kushiro
authors.affiliation: National Agriculture and Food Research Organization (NARO), National Food Research Institute, 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan. nagasima@affrc.go.jp.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nagashima
authors.affiliation: National Agriculture and Food Research Organization (NARO), National Food Research Institute, 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan. hironkgw@affrc.go.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakagawa
mesh_terms: D002853:Chromatography, Liquid; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D037341:Fumonisins / Q000302:isolation & purification*; D013058:Mass Spectrometry; D003313:Zea mays / Q000382:microbiology*
chemical_list: D037341:Fumonisins; C056934:fumonisin B2; C073063:fumonisin B3
keywords: Fusarium; LC-Orbitrap MS; N-(1-deoxy-d-fructos-1-yl) fumonisin; corn; fumonisin