title: Generation of Th17 cells in response to intranasal infection requires TGF-β1 from dendritic cells and IL-6 from CD301b+ dendritic cells.
abstract: Intranasal (i.n.) infections preferentially generate Th17 cells. We explored the basis for this anatomic preference by tracking polyclonal CD4(+) T cells specific for an MHC class II-bound peptide from the mucosal pathogen Streptococcus pyogenes. S. pyogenes MHC class II-bound peptide-specific CD4(+) T cells were first activated in the cervical lymph nodes following i.n. inoculation and then differentiated into Th17 cells. S. pyogenes-induced Th17 formation depended on TGF-β1 from dendritic cells and IL-6 from a CD301b(+) dendritic cell subset located in the cervical lymph nodes but not the spleen. Thus, the tendency of i.n. infection to induce Th17 cells is related to cytokine production by specialized dendritic cells that drain this site.
authors.affiliation: Department of Microbiology and Immunology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455;
authors.forename: Jonathan L
authors.initials: JL
authors.lastname: Linehan
authors.affiliation: Department of Microbiology and Immunology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455;
authors.forename: Thamotharampillai
authors.initials: T
authors.lastname: Dileepan
authors.affiliation: Department of Dermatology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455.
authors.forename: Sakeen W
authors.initials: SW
authors.lastname: Kashem
authors.affiliation: Department of Dermatology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455.
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Kaplan
authors.affiliation: Department of Microbiology and Immunology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455;
authors.forename: Patrick
authors.initials: P
authors.lastname: Cleary
authors.affiliation: Department of Microbiology and Immunology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455; jenki002@umn.edu.
authors.forename: Marc K
authors.identifier: http://orcid.org/0000-0001-8009-7655
authors.initials: MK
authors.lastname: Jenkins
mesh_terms: D000818:Animals; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D007111:Immunity, Cellular; D015850:Interleukin-6 / Q000276:immunology*; D037181:Lectins, C-Type / Q000276:immunology*; D051379:Mice; D009668:Nose Diseases / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D013290:Streptococcal Infections / Q000276:immunology* / Q000473:pathology; D013297:Streptococcus pyogenes / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology* / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000276:immunology*
chemical_list: D015850:Interleukin-6; D037181:Lectins, C-Type; C464940:MGL2 protein, mouse; C508961:Tgfb1 protein, mouse; D053773:Transforming Growth Factor beta1; C577686:interleukin-6, mouse
keywords: CD301b+; Streptococcus pyogenes; Th17; dendritic cells