title: Constructing comprehensive venom proteome reference maps for integrative venomics.
abstract: OBJECTIVE
  Understanding the molecular basis of complex adaptive traits, such as snake venom, demands qualitative and quantitative comparisons of the temporal and spatial patterns of venom variation. Here, we assessed the proof-of-concept that locus-resolved reference venom proteome maps can be achieved through efficient pre-MS venom proteome decomplexation, peptide-centric MS/MS analysis and species-specific database searching.
  
  
  METHODS
  Venom proteome components were fractionated and quantified by RP-HPLC, SDS-PAGE and 2DE prior to LC-MS/MS matching against a species-specific transcriptomic dataset.
  
  
  RESULTS
  Combination of RP-HPLC/SDS-PAGE and 2DE followed by LC-MS/MS showed the existence of ∼178-180 venom protein species generated from ∼48 unique transcripts.
  
  
  CONCLUSIONS
  Our results underscore that if sufficient pre-MS and MS efforts are applied, comprehensive venom maps can be achieved. And - equally important - dissociating the venom decomplexing steps from the protein identification process represents the key to achieving a quantitative and locus-resolved insight of the venom proteome.
authors.affiliation: a 1 Beuth Hochschule für Technik Berlin, Luxemburger Straße 10, 13353 Berlin, Germany.
authors.forename: Susann
authors.initials: S
authors.lastname: Eichberg
authors.affiliation: b 2 Laboratorio de Venómica Estructural y Funcional, Instituto de Biomedicina de Valencia, CSIC, Valencia, Spain.
authors.forename: Libia
authors.initials: L
authors.lastname: Sanz
authors.affiliation: b 2 Laboratorio de Venómica Estructural y Funcional, Instituto de Biomedicina de Valencia, CSIC, Valencia, Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Calvete
authors.affiliation: b 2 Laboratorio de Venómica Estructural y Funcional, Instituto de Biomedicina de Valencia, CSIC, Valencia, Spain.
authors.forename: Davinia
authors.initials: D
authors.lastname: Pla
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000737:chemistry*; D020543:Proteome / Q000737:chemistry*
chemical_list: D003435:Crotalid Venoms; D020543:Proteome
keywords: Crotalus adamanteus; mass spectrometry; reverse phase-HPLC; snake venom proteomics; two-dimensional electrophoresis; venomics