title: A Microflow Cytometer Based on a Disposable Microfluidic Chip With Side Scatter and Fluorescence Detection Capability.
abstract: In this paper, we present a microflow cytometer based on a polydimethylsiloxane (PDMS) microfluidic chip. The PDMS chip has a smooth sidewall because of the proposed double molding process. Thus, the side scatter (SSC) detection can be used for the cytometer. The coincidence error using the sheathless focus mechanism is less than 0.069%. Using Flow-Check alignment beads for the counting experiment, the coefficient of variation (CV) of the SSC and the fluorescein isothiocyanate (FITC) channel can reach 8.37% and 2.46%, respectively. The gating of the lymphocytes according to CD45/SSC was also demonstrated successfully. It proves that the chip is a promising solution for point of care (POC) microflow cytometers.
authors.forename: Wenpeng
authors.initials: W
authors.lastname: Xun
authors.forename: Jianguo
authors.initials: J
authors.lastname: Feng
authors.forename: Honglong
authors.initials: H
authors.lastname: Chang
mesh_terms: D004129:Dimethylpolysiloxanes; D004867:Equipment Design; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009757:Nylons
chemical_list: D004129:Dimethylpolysiloxanes; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer