title: Functional and regenerative effects of local administration of autologous mononuclear bone marrow cells combined with silicone conduit on transected femoral nerve of rabbits.
abstract: The inoculation of cells into injury sites can accelerate and improve the quality of nerve regeneration. This study aimed to evaluate the functional and regenerative effects of mononuclear autologous bone marrow cells (MABMC) combined with silicon conduit grafting in rabbit femoral nerves. Twenty-eight animals were allocated to one of two groups: treatment group (TG) or control group (CG), divided according to the time of evaluation, at either 50 or 75 days. After neurotmesis of the femoral nerve, surgical repair was performed with nerve autografts in silicon conduits, leaving a 5mm gap in both groups. The TG received MABMC in silicon conduits, and CG received a sham saline inoculum. Histological, clinical and electrophysiological analyses detected no differences between groups, but analysis of leg diameter showed that TG diameters were larger. This cell therapy did not improve regeneration of the femoral nerve, but there was a tendency for better functional recovery.
authors.affiliation: Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. Electronic address: anelisebt@yahoo.com.br.
authors.forename: Anelise Bonilla
authors.initials: AB
authors.lastname: Trindade
authors.affiliation: Neurology Department, Eletroneuromyography, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil.
authors.forename: Pedro
authors.initials: P
authors.lastname: Schestatsky
authors.affiliation: Neurology Department, Eletroneuromyography, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil.
authors.forename: Vítor Félix
authors.initials: VF
authors.lastname: Torres
authors.affiliation: Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Cristiano
authors.initials: C
authors.lastname: Gomes
authors.affiliation: Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Giordano Cabral
authors.initials: GC
authors.lastname: Gianotti
authors.affiliation: Laboratory of Embryology and Cell Differentiation, CPE, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Ana Helena da Rosa
authors.initials: AH
authors.lastname: Paz
authors.affiliation: Laboratory of Embryology and Cell Differentiation, CPE, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Paula Barros
authors.initials: PB
authors.lastname: Terraciano
authors.affiliation: Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Janete Maria Volpato
authors.initials: JM
authors.lastname: Marques
authors.affiliation: Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Karina Magano
authors.initials: KM
authors.lastname: Guimarães
authors.affiliation: Laboratory Animal Pathology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Dominguita Lühers
authors.initials: DL
authors.lastname: Graça
authors.affiliation: Laboratory of Embryology and Cell Differentiation, CPE, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Elizabeth Obino
authors.initials: EO
authors.lastname: Cirne-Lima
authors.affiliation: Department of Animal Medicine, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Emerson Antonio
authors.initials: EA
authors.lastname: Contesini
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D016026:Bone Marrow Transplantation / Q000662:veterinary*; D005267:Femoral Nerve / Q000293:injuries*; D009416:Nerve Regeneration / Q000502:physiology*; D019919:Prosthesis Implantation; D011817:Rabbits; D020127:Recovery of Function; D012828:Silicones; D014182:Transplantation, Autologous
chemical_list: D012828:Silicones
keywords: Autograft; Autologous mononuclear cell; Cell therapy; Femoral nerve regeneration; Microsurgery