title: Zinc Induces Apoptosis of Human Melanoma Cells, Increasing Reactive Oxygen Species, p53 and FAS Ligand.
abstract: The aim of this study was to examine the in vitro effect of zinc on the apoptosis of human melanoma cells, by studying the zinc-dependent modulation of intracellular levels of reactive oxygen species (ROS) and of p53 and FAS ligand proteins. We showed that zinc concentrations ranging from 33.7 μM to 75 μM Zn(2+) induced apoptosis in the human melanoma cell line WM 266-4. This apoptosis was associated with an increased production of intracellular ROS, and of p53 and FAS ligand protein. Treatment of tumor cells with the antioxidant N-acetylcysteine was able to prevent Zn(2+)-induced apoptosis, as well as the increase of p53 and FAS ligand protein induced by zinc. Zinc induces apoptosis in melanoma cells by increasing ROS and this effect may be mediated by the ROS-dependent induction of p53 and FAS/FAS ligand.
authors.affiliation: Advanced Technology Center for Aging Research, Scientific Technological Area, Italian National Research Centers on Aging (I.N.R.C.A.), Ancona, Italy m.provinciali@inrca.it.
authors.forename: Mauro
authors.initials: M
authors.lastname: Provinciali
authors.affiliation: Advanced Technology Center for Aging Research, Scientific Technological Area, Italian National Research Centers on Aging (I.N.R.C.A.), Ancona, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Pierpaoli
authors.affiliation: Advanced Technology Center for Aging Research, Scientific Technological Area, Italian National Research Centers on Aging (I.N.R.C.A.), Ancona, Italy.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Bartozzi
authors.affiliation: Advanced Technology Center for Aging Research, Scientific Technological Area, Italian National Research Centers on Aging (I.N.R.C.A.), Ancona, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Bernardini
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D053222:Fas Ligand Protein / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008545:Melanoma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012878:Skin Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D017382:Reactive Oxygen Species; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D015032:Zinc; D000111:Acetylcysteine
keywords: FAS ligand; Melanoma; apoptosis; p53; reactive oxygen species; zinc