title: Extracellular vesicle-mediated transfer of processed and functional RNY5 RNA.
abstract: Extracellular vesicles (EVs) have been proposed as a means to promote intercellular communication. We show that when human primary cells are exposed to cancer cell EVs, rapid cell death of the primary cells is observed, while cancer cells treated with primary or cancer cell EVs do not display this response. The active agents that trigger cell death are 29- to 31-nucleotide (nt) or 22- to 23-nt processed fragments of an 83-nt primary transcript of the human RNY5 gene that are highly likely to be formed within the EVs. Primary cells treated with either cancer cell EVs, deproteinized total RNA from either primary or cancer cell EVs, or synthetic versions of 31- and 23-nt fragments trigger rapid cell death in a dose-dependent manner. The transfer of processed RNY5 fragments through EVs may reflect a novel strategy used by cancer cells toward the establishment of a favorable microenvironment for their proliferation and invasion.
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
authors.forename: Sudipto K
authors.initials: SK
authors.lastname: Chakrabortty
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
authors.forename: Ashwin
authors.initials: A
authors.lastname: Prakash
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
authors.forename: Gal
authors.initials: G
authors.lastname: Nechooshtan
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Hearn
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Gingeras
mesh_terms: D002450:Cell Communication / Q000502:physiology; D016923:Cell Death / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D000067128:Extracellular Vesicles / Q000378:metabolism*; D006801:Humans; D020014:K562 Cells; D009369:Neoplasms / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*
chemical_list: D012313:RNA
keywords: RNY5; cancer microenvironment; exosomes; extracellular vesicles