title: Protective Effect of Diphlorethohydroxycarmalol against Ultraviolet B Radiation-Induced DNA Damage by Inducing the Nucleotide Excision Repair System in HaCaT Human Keratinocytes.
abstract: We investigated the protective properties of diphlorethohydroxycarmalol (DPHC), a phlorotannin, against ultraviolet B (UVB) radiation-induced cyclobutane pyrimidine dimers (CPDs) in HaCaT human keratinocytes. The nucleotide excision repair (NER) system is the pathway by which cells identify and repair bulky, helix-distorting DNA lesions such as ultraviolet (UV) radiation-induced CPDs and 6-4 photoproducts. CPDs levels were elevated in UVB-exposed cells; however, this increase was reduced by DPHC. Expression levels of xeroderma pigmentosum complementation group C (XPC) and excision repair cross-complementing 1 (ERCC1), which are essential components of the NER pathway, were induced in DPHC-treated cells. Expression of XPC and ERCC1 were reduced following UVB exposure, whereas DPHC treatment partially restored the levels of both proteins. DPHC also increased expression of transcription factor specificity protein 1 (SP1) and sirtuin 1, an up-regulator of XPC, in UVB-exposed cells. DPHC restored binding of the SP1 to the XPC promoter, which is reduced in UVB-exposed cells. These results indicate that DPHC can protect cells against UVB-induced DNA damage by inducing the NER system.
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Korea. meijing0219@hotmail.com.
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Korea. susaramh@gmail.com.
authors.forename: Susara Ruwan Kumara Madduma
authors.initials: SR
authors.lastname: Hewage
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Korea. hanx55@hotmail.com.
authors.forename: Xia
authors.initials: X
authors.lastname: Han
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Korea. legna07@naver.com.
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Korea. pharmkhk@jejunu.ac.kr.
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Kang
authors.affiliation: Department of Chemistry, College of Natural Sciences, Jeju National University, Jeju 63243, Korea. namho@jejunu.ac.kr.
authors.forename: Nam Ho
authors.initials: NH
authors.lastname: Lee
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Korea. jinwonh@jejunu.ac.kr.
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D002460:Cell Line; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D006575:Heterocyclic Compounds, 3-Ring / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D006575:Heterocyclic Compounds, 3-Ring; C513811:diphlorethohydroxycarmalol
keywords: cyclobutane pyrimidine dimmers; diphlorethohydroxycarmalol; excision repair cross-complementing 1; ultraviolet B; xeroderma pigmentosum complementation group C