title: FAS ligand expression in inflammatory infiltrate lymphoid cells as a prognostic marker in oral squamous cell carcinoma.
abstract: Currently, the most important prognostic factor in oral squamous cell carcinoma (OSCC) is the presence of regional lymph node metastases, which correlates with a 50% reduction in life expectancy. We have previously observed that expression of hypoxia genes in the tumor inflammatory infiltrate is statistically related to prognosis in OSCC. FAS and FASL expression levels in OSCC have previously been related to patient survival. The present study analyzed the relationship between FASL expression in the inflammatory infiltrate lymphoid cells and clinical variables, tumor histology, and prognosis of OSCC. Strong FASL expression was significantly associated with lymph node metastases (P = 0.035) and disease-specific death (P = 0.014), but multivariate analysis did not confirm FASL expression as an independent death risk factor (OR = 2.78, 95%CI = 0.81-9.55). Disease-free and disease-specific survival were significantly correlated with FASL expression (P = 0.016 and P = 0.005, respectively). Multivariate analysis revealed that strong FASL expression is an independent marker for earlier disease relapse and disease-specific death, with approximately 2.5-fold increased risk compared with weak expression (HR = 2.24, 95%CI = 1.08-4.65 and HR = 2.49, 95%CI = 1.04-5.99, respectively). Our results suggest a potential role for this expression profile as a tumor prognostic marker in OSCC patients.
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: G T
authors.initials: GT
authors.lastname: Peterle
authors.affiliation: Departamento de Medicina, Universidade Federal do Rio Grande do Norte, Campus Caicó, Caicó, RN, Brasil.
authors.forename: M
authors.initials: M
authors.lastname: Santos
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: S O
authors.initials: SO
authors.lastname: Mendes
authors.affiliation: Laboratório de Biologia Molecular, Hospital Heliópolis, São Paulo, SP, Brasil.
authors.forename: P B
authors.initials: PB
authors.lastname: Carvalho-Neto
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: L L
authors.initials: LL
authors.lastname: Maia
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: E
authors.initials: E
authors.lastname: Stur
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: L P
authors.initials: LP
authors.lastname: Agostini
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: C V M
authors.initials: CV
authors.lastname: Silva
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: L O
authors.initials: LO
authors.lastname: Trivilin
authors.affiliation: Departamento de Patologia Bucal, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: F D
authors.initials: FD
authors.lastname: Nunes
authors.affiliation: Laboratório de Biologia Molecular, Hospital Heliópolis, São Paulo, SP, Brasil.
authors.forename: M B
authors.initials: MB
authors.lastname: Carvalho
authors.affiliation: Departamento de Biologia Molecular, Faculdade de Medicina, São José do Rio Preto, SP, Brasil.
authors.forename: E H
authors.initials: EH
authors.lastname: Tajara
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: I D
authors.initials: ID
authors.lastname: Louro
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: A M A
authors.initials: AM
authors.lastname: Silva-Conforti
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000556:secondary; D053222:Fas Ligand Protein / Q000378:metabolism*; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D016246:Lymphocytes, Tumor-Infiltrating / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D015999:Multivariate Analysis; D011379:Prognosis; D016016:Proportional Hazards Models
chemical_list: D014408:Biomarkers, Tumor; C505661:FASLG protein, human; D053222:Fas Ligand Protein