title: Effects of S53P4 bioactive glass on osteoblastic cell and biomaterial surface interaction.
abstract: To study the effect of bioactive glass bone substitute granules (S53P4) and hypoxic atmospheric conditions on human osteoblastic cell adhesion on different biomaterials. Cellular adhesion and cytoskeletal organization were studied on titanium, polytetrafluoroethylene, polydimethylsiloxane and S53P4 plates in the presence or absence of S53P4 granules. Cells used were human osteoblast-like SaOS-2 cells. The experiments were done either in normal atmospheric conditions or in hypoxia which simulates conditions prevailing in chronically infected bone or bone cavities. Vinculin-containing focal adhesions, organization of actin cytoskeleton and nuclear staining of cells on biomaterial surfaces were studied at 4.5 h, 2 and 4 days. In normoxic conditions S53P4 granules alkalinized the cell culture medium but cellular adhesion and cytoskeletal organization were usually not affected by their presence. Hypoxic conditions associated with lower pH and impaired cellular adhesion, vinculin-containing focal adhesion formation and rearrangement of the actin filaments to actin cytoskeleton. On most materials studied in hypoxic conditions, however, S53P4 granules prevented this impairment of cellular adhesion and cytoskeletal reorganization. The S53P4 granules promote the adhesion of SaOS-2 cells to various biomaterial surfaces especially in hypoxic conditions, in which S53P4 granules increase pH. The presence of S53P4 granules may protect biomaterial surface from bacterial colonization and promote osteointegration of implants used together with S53P4 granules for fixation and weight bearing.
authors.affiliation: Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Haartmaninkatu 4 E, 00029 HUS, Helsinki, Finland. ramontanoira@hotmail.com.|Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain. ramontanoira@hotmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Pérez-Tanoira
authors.affiliation: Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Haartmaninkatu 4 E, 00029 HUS, Helsinki, Finland.
authors.forename: T J
authors.initials: TJ
authors.lastname: Kinnari
authors.affiliation: Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Haartmaninkatu 4 E, 00029 HUS, Helsinki, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Hyyrynen
authors.affiliation: ORTON Research Institute, Helsinki, Finland.|ORTON Orthopedic Hospital, Helsinki, Finland.
authors.forename: A
authors.initials: A
authors.lastname: Soininen
authors.affiliation: Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Haartmaninkatu 4 E, 00029 HUS, Helsinki, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Pietola
authors.affiliation: ORTON Research Institute, Helsinki, Finland.|ORTON Orthopedic Hospital, Helsinki, Finland.
authors.forename: V-M
authors.initials: VM
authors.lastname: Tiainen
authors.affiliation: ORTON Research Institute, Helsinki, Finland.|ORTON Orthopedic Hospital, Helsinki, Finland.|Department of Medicine, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.|COXA Hospital for Joint Replacement, Tampere, Finland.
authors.forename: Y T
authors.initials: YT
authors.lastname: Konttinen
authors.affiliation: Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Haartmaninkatu 4 E, 00029 HUS, Helsinki, Finland.
authors.forename: A A
authors.initials: AA
authors.lastname: Aarnisalo
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D018786:Bone Substitutes / Q000737:chemistry; D002448:Cell Adhesion; D015687:Cell Hypoxia / Q000502:physiology; D002460:Cell Line; D004129:Dimethylpolysiloxanes; D022001:Focal Adhesions / Q000378:metabolism; D005898:Glass; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D011138:Polytetrafluoroethylene; D013499:Surface Properties; D014025:Titanium; D016596:Vinculin / Q000378:metabolism
chemical_list: D018786:Bone Substitutes; D004129:Dimethylpolysiloxanes; C403922:bioactive glass S53P4; D016596:Vinculin; C013830:baysilon; D011138:Polytetrafluoroethylene; D014025:Titanium