title: Cotherapy of Tiron and selenium against vanadium induced toxic effects in lactating rats.
abstract: BACKGROUND
  Vanadium is an important environmental and industrial pollutant. It has a status of reproductive toxicant and is reported to cross placental barrier.
  
  
  OBJECTIVE
  The current study was performed to assess the therapeutic efficacy of Tiron and its combination with selenium against vanadium induced toxicity in lactating and suckling rats.
  
  
  METHODS
  Rats were exposed to vanadium at a dose of 7.5 mg/kg/day (p.o.) for 20 days from 0 day of post partom (p.p.). Tiron (606 mg/kg/day, i.p.) and selenium (0.5 mg/kg/day, p.o.) were administered for 5 days on 21-25 day PP.
  
  
  RESULTS
  Vanadium exposure decreased blood sugar level while serum transaminases and serum alkaline phosphatase showed increased values significantly (p<0.01). Elevation in glycogen content of liver and kidney of suckling and kidney of lactating rats was found after toxicant administration. Toxicant intoxication increased the enzymatic activity of acid phosphatase in liver of suckling and lactating and kidney of suckling rats. On the contrary alkaline phosphatase and adenosine triphosphatase activities were inhibited significantly (p<0.01) in all the organs. Lipid peroxidation was enhanced whereas glutathione was reduced significantly in liver of suckling and lactating rats (p<0.01). Vanadium also caused histopathological lesions. Therapies of Tiron per se and Tiron along with selenium maintained almost all blood and tissue biochemical parameters towards normal. Tiron along with selenium reduced vanadium induced lesions in lactating and sucklings rats.
  
  
  CONCLUSIONS
  Tiron along with selenium is more effective than Tiron alone against vanadium induced toxic effect on lactating and suckling rats.
authors.affiliation: UNESCO Satellite Center of Trace Element Research and Reproductive Biology and Toxicology Laboratory, School of Studies in Zoology, Jiwaji University, Gwalior (MP), 474 011, India.
authors.forename: Sadhana
authors.initials: S
authors.lastname: Shrivastava
authors.affiliation: UNESCO Satellite Center of Trace Element Research and Reproductive Biology and Toxicology Laboratory, School of Studies in Zoology, Jiwaji University, Gwalior (MP), 474 011, India.
authors.forename: Deepmala
authors.initials: D
authors.lastname: Joshi
authors.affiliation: UNESCO Satellite Center of Trace Element Research and Reproductive Biology and Toxicology Laboratory, School of Studies in Zoology, Jiwaji University, Gwalior (MP), 474 011, India.
authors.forename: Monika
authors.initials: M
authors.lastname: Bhadauria
authors.affiliation: UNESCO Satellite Center of Trace Element Research and Reproductive Biology and Toxicology Laboratory, School of Studies in Zoology, Jiwaji University, Gwalior (MP), 474 011, India.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Shukla
authors.affiliation: UNESCO Satellite Center of Trace Element Research and Reproductive Biology and Toxicology Laboratory, School of Studies in Zoology, Jiwaji University, Gwalior (MP), 474 011, India.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Mathur
keywords: Suckling; Teratogenecity; Tiron; Vanadium; lactating; selenium