title: [Diagnostic tools for canine parvovirus infection].
abstract: Canine parvovirus (CPV) infection is one of the most important and common infectious diseases in dogs, in particular affecting young puppies when maternal antibodies have waned and vaccine-induced antibodies have not yet developed. The mortality rate remains high. Therefore, a rapid and safe diagnostic tool is essential to diagnose the disease to 1) provide intensive care treatment and 2) to identify virus-shedding animals and thus prevent virus spread. Whilst the detection of antibodies against CPV is considered unsuitable to diagnose the disease, there are several different methods to directly detect complete virus, virus antigen or DNA. Additionally, to test in commercial laboratories, rapid in-house tests based on ELISA are available worldwide. The specificity of the ELISA rapid in-house tests is reported to be excellent. However, results on sensitivity vary and high numbers of false-negative results are commonly reported, which potentially leads to misdiagnosis. Polymerase chain reaction (PCR) is a very sensitive and specific diagnostic tool. It also provides the opportunity to differentiate vaccine strains from natural infection when sequencing is performed after PCR.
authors.forename: A L
authors.initials: AL
authors.lastname: Proksch
authors.affiliation: Prof. Dr. Katrin Hartmann, Medizinische Kleintierklinik, Ludwig-Maximilians-Universität München, Veterinärstraße 13, 80539 München, E-Mail: hartmann@lmu.de.
authors.forename: K
authors.initials: K
authors.lastname: Hartmann
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D004283:Dog Diseases / Q000175:diagnosis* / Q000821:virology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017992:Parvovirus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
chemical_list: D000914:Antibodies, Viral
keywords: CPV; Dog; ELISA; PCR; antibodies; direct pathogen test; parvovirosis; rapid in-house test