title: Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha.
abstract: We previously reported Agrobacterium-mediated transformation methods for the liverwort Marchantia polymorpha using the hygromycin phosphotransferase gene as a marker for selection with hygromycin. In this study, we developed three additional markers for M. polymorpha transformation: the gentamicin 3'-acetyltransferase gene for selection with gentamicin; a mutated acetolactate synthase gene for selection with chlorsulfuron; and the neomycin phosphotransferase II gene for selection with G418. Based on these four marker genes, we have constructed a series of Gateway binary vectors designed for transgenic experiments on M. polymorpha. The 35S promoter from cauliflower mosaic virus and endogenous promoters for constitutive and heat-inducible expression were used to create these vectors. The reporters and tags used were Citrine, 3×Citrine, Citrine-NLS, TagRFP, tdTomato, tdTomato-NLS, GR, SRDX, SRDX-GR, GUS, ELuc(PEST), and 3×FLAG. These vectors, designated as the pMpGWB series, will facilitate molecular genetic analyses of the emerging model plant M. polymorpha.
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kyoto, Japan; Graduate School of Science, Kobe University, Kobe, Japan.
authors.forename: Kimitsune
authors.initials: K
authors.lastname: Ishizaki
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Nishihama
authors.affiliation: Department of Botany, Graduate School of Science, Kyoto University, Kyoto, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Ueda
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Inoue
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
authors.forename: Sakiko
authors.initials: S
authors.lastname: Ishida
authors.affiliation: Department of Botany, Graduate School of Science, Kyoto University, Kyoto, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Nishimura
authors.affiliation: Department of Botany, Graduate School of Science, Kyoto University, Kyoto, Japan.
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Shikanai
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kohchi
mesh_terms: D000095:Acetolactate Synthase / Q000235:genetics / Q000378:metabolism*; D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D016960:Agrobacterium tumefaciens / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005819:Genetic Markers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D005839:Gentamicins / Q000494:pharmacology; D019868:Kanamycin Kinase / Q000235:genetics / Q000378:metabolism*; D047008:Marchantia / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000254:growth & development; D013449:Sulfonamides / Q000494:pharmacology; D014162:Transfection; D014227:Triazines / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D005819:Genetic Markers; D005839:Gentamicins; D013449:Sulfonamides; D014227:Triazines; C010680:antibiotic G 418; D000095:Acetolactate Synthase; D000123:Acetyltransferases; C020564:gentamicin 3-acetyltransferase; D019868:Kanamycin Kinase; C037137:chlorsulfuron