title: Distribution of Antimicrobial Resistance in Campylobacter Strains Isolated from Poultry at a Slaughterhouse and Supermarkets in Japan.
abstract: Fifty strains of Campylobacter jejuni/coli were detected in 108 specimens of chicken meat and organs sampled at six supermarkets and one poultry slaughterhouse (large scale) between April and October 2013 (isolation rates: 84.8% from the slaughterhouse, 29.3% from the supermarkets). 46/50 strains were successfully recovered and subjected to the E-test to examine their susceptibility to three fluoroquinolone antibacterial agents authorized for use in poultry in Japan: enrofloxacin (ERFX), ofloxacin (OFLX), and norfloxacin (NLFX). 29 isolates (63%) were resistant to all three agents and 2 isolates (4.3%) were resistant to two agents (ERFX and OFLX). The resistance rates of strains isolated fom the supermarkets and slaughterhouse were 61.9% and 72.0%, respectively. Because the chickens processed at the slaughterhouse were raised without the use of fluoroquinolone, the results did not suggest a positive relationship between the use of these agents and the distribution of antimicrobial-resistant bacteria. Susceptibility to macrolide antibiotics (erythromycin [EM]) was also tested in 42 strains, and one strain (2.4%), C. coli from a retailer sample, showed resistance. Previous studies have detected high rates of fluoroquinolone-resistant strains, suggesting an expanding distribution of resistant bacteria. The detection of EM-resistant bacteria downstream in the food distribution chain (i.e., closer to consumers) is a concern for human health.
authors.affiliation: Department of Microbiology, Gifu University School of Medicine.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kojima
authors.forename: Michiru
authors.initials: M
authors.lastname: Kishimoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ezaki
mesh_terms: D000003:Abattoirs; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017000:Campylobacter coli / Q000187:drug effects* / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000187:drug effects* / Q000302:isolation & purification; D002645:Chickens; D052978:Disk Diffusion Antimicrobial Tests; D024881:Drug Resistance, Bacterial; D004917:Erythromycin / Q000494:pharmacology*; D024841:Fluoroquinolones / Q000494:pharmacology*; D007564:Japan; D008460:Meat / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D004917:Erythromycin