title: Vitamin D metabolite profiling using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
abstract: Liquid chromatography tandem mass spectrometry (LC-MS/MS) has emerged as the latest technology to be used to assay the metabolites of vitamin D. The method uses molecular mass as a detection technique after straightforward extraction and chromatography steps. LC-MS/MS assay provides a level of accuracy and reproducibility not seen before with other methods and is beginning to rival antibody-based methods in terms of sensitivity and convenience. Methods for detection of underivatized and DMEQ-TAD derivatized vitamin D metabolites are evaluated. Sensitivity is improved by 10-100 fold with derivatization and allows for the simultaneous assay of multiple vitamin D metabolites, a process termed vitamin D metabolite profiling. Clinical and research applications of vitamin D metabolite profiling are discussed.
authors.affiliation: Department of Biomedical & Molecular Sciences, Queen's University, Kingston, Ontario K7L3N6, Canada.
authors.forename: Glenville
authors.initials: G
authors.lastname: Jones
authors.affiliation: Department of Biomedical & Molecular Sciences, Queen's University, Kingston, Ontario K7L3N6, Canada. Electronic address: gj1@queensu.ca.
authors.forename: Martin
authors.initials: M
authors.lastname: Kaufmann
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002112:Calcifediol / Q000378:metabolism; D002853:Chromatography, Liquid; D005260:Female; D005838:Genotype; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D011485:Protein Binding; D011810:Quinoxalines / Q000737:chemistry; D011863:Radioimmunoassay; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D014230:Triazoles / Q000737:chemistry; D014807:Vitamin D / Q000378:metabolism*
chemical_list: D011810:Quinoxalines; D014230:Triazoles; C070204:4-(2-(6,7-dimethoxy-4-methyl-3-oxo-3,4-dihydroquinoxalinyl)ethyl)-1,2,4-triazoline-3,5-dione; D014807:Vitamin D; D002112:Calcifediol
keywords: 25-OH-D(3); DMEQ-TAD; LC–MS/MS; Metabolomics; Vitamin D assay