title: [Effect of DNase I on biofilm formation of Staphylococcus aureus].
abstract: OBJECTIVE
  To study the effect of DNase I on biofilm formation of Staphylococcus aureus.
  
  
  METHODS
  The growth curve of S. aureus was detected using a spectrophotometer. The adhesion of S. aureus was analyzed using flat colony counting method, and the biofilm formation was assayed using the 96-well crystal violet staining method.
  
  
  RESULTS
  Exposure to different concentrations of DNase I did not obviously affect the growth of S. aureus but significantly inhibit the formation of bacterial biofilms in a dose-dependent manner. DNase I inhibited the adhesion of S. aureus at different growth stages. When combined with antibiotics, DNase I resulted in a signi?cant decrease in the established bio?lm biomass compared to antibiotics or DNase I used alone.
  
  
  CONCLUSIONS
  DNase I can effectively inhibit biofilm formation of S. aureus and enhance the inhibitory effect of antibiotics against S. aureus biofilms.
authors.affiliation: Health Division of Guard Bureau, General Staff Department of PLA, Beijing 100017, China.E-mail: caixiaobing5757@hotmail.com.
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Xu
authors.forename: Fengjun
authors.initials: F
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Feng
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Yawei
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000900:Anti-Bacterial Agents; D018441:Biofilms / Q000187:drug effects*; D003850:Deoxyribonuclease I / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000254:growth & development*
chemical_list: D000900:Anti-Bacterial Agents; D003850:Deoxyribonuclease I