title: Optimization of diacylglycerol production by glycerolysis of fish oil catalyzed by Lipozyme TL IM with Tween 65.
abstract: The diacylglycerols (DAG) are emulsifiers with high added value used as functional additives in food, medicine, and cosmetic industries. In glycerolysis of oils for the production of DAG, the immiscibility between the substrates (glycerol and oil phases) has to be overcome, for example, by the addition of a food grade surfactant like Tween 65. The main objective of this work was to optimize the process conditions for obtaining diacylglycerols rich in the omega-3 eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, through the enzymatic glycerolysis of fish oil, in systems with Tween 65 and without this surfactant, using Lipozyme TL(®) IM as biocatalyst. The experiments were performed according to predetermined conditions varying the temperature, enzyme load, the oil to glycerol molar ratio and, when added, the surfactant load. After 6 h of reaction, it was possible to produce up to 20.76 and 13.76% of diacylglycerols from fish oil in the reactions with and without Tween 65, respectively.
authors.affiliation: Food Engineering Department, Universidade do Estado de Santa Catarina (UDESC), Florianópolis, SC, 88035-001, Brazil. soelifrancisca@gmail.com.|Chemical Engineering and Food Engineering Department, Universidade Federal de Santa Catarina, Campus Universitário, CP 476, Florianópolis, SC, 88040-900, Brazil. soelifrancisca@gmail.com.
authors.forename: S F M
authors.initials: SF
authors.lastname: Monte Blanco
authors.affiliation: Chemical Engineering and Food Engineering Department, Universidade Federal de Santa Catarina, Campus Universitário, CP 476, Florianópolis, SC, 88040-900, Brazil.
authors.forename: J S
authors.initials: JS
authors.lastname: Santos
authors.affiliation: Instituto Federal de Educação, Ciência e Tecnologia Catarinense, IFC, Brusque, SC, 88350-301, Brazil. mmanuelafeltes@hotmail.com.
authors.forename: M M C
authors.initials: MM
authors.lastname: Feltes
authors.affiliation: Chemical Engineering and Food Engineering Department, Universidade Federal de Santa Catarina, Campus Universitário, CP 476, Florianópolis, SC, 88040-900, Brazil.
authors.forename: G
authors.initials: G
authors.lastname: Dors
authors.affiliation: Chemical Engineering and Food Engineering Department, Universidade Federal de Santa Catarina, Campus Universitário, CP 476, Florianópolis, SC, 88040-900, Brazil.
authors.forename: S
authors.initials: S
authors.lastname: Licodiedoff
authors.affiliation: Chemical Engineering and Food Engineering Department, Universidade Federal de Santa Catarina, Campus Universitário, CP 476, Florianópolis, SC, 88040-900, Brazil.
authors.forename: L A
authors.initials: LA
authors.lastname: Lerin
authors.affiliation: Chemical Engineering and Food Engineering Department, Universidade Federal de Santa Catarina, Campus Universitário, CP 476, Florianópolis, SC, 88040-900, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: de Oliveira
authors.affiliation: Chemical Engineering and Food Engineering Department, Universidade Federal de Santa Catarina, Campus Universitário, CP 476, Florianópolis, SC, 88040-900, Brazil.
authors.forename: J L
authors.initials: JL
authors.lastname: Ninow
authors.affiliation: Chemical Engineering and Food Engineering Department, Universidade Federal de Santa Catarina, Campus Universitário, CP 476, Florianópolis, SC, 88040-900, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Furigo
mesh_terms: D002384:Catalysis; D004075:Diglycerides / Q000096:biosynthesis*; D005395:Fish Oils / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D004075:Diglycerides; D005395:Fish Oils; D013501:Surface-Active Agents; C093666:Lipozyme; D008049:Lipase; D005990:Glycerol
keywords: Biocatalysis; Diacylglycerols; Lipase; Omega-3; Surfactant