title: Application of a Collagenated Biphasic Calcium Phosphate Loaded with Fibroblast Growth Factor-2 in the Rabbit Sinus: A Pilot Study.
abstract: OBJECTIVE
  The aim of this study was to determine the effect of collagenated biphasic calcium phosphate (CBCP) loaded with fibroblast growth factor-2 (FGF-2) on bone regeneration in a rabbit sinus model.
  
  
  METHODS
  Bilateral sinus augmentations were performed using either FGF-2-loaded CBCP (FGF-2 group) or CBCP only (control group) in 10 rabbits. The experimental animals were euthanized at 2 weeks (n = 5) or 4 weeks (n = 5) postoperatively. Volumetric analyses were done by means of microcomputed tomography and histomorphometry.
  
  
  RESULTS
  The volumes of total augmentation, new bone, residual material, and fibrovascular tissue were significantly greater in the FGF-2 group than in the control group at 2 weeks, but the differences between the two groups had disappeared by 4 weeks. Histologically, the healing patterns, such as new bone formation and the resorption of residual material, did not differ notably between the two groups. The results of the histometric analysis did not correspond to those of the volumetric analysis. The areas of total augmentation, new bone, residual material, and fibrovascular tissue, and the number of blood vessels did not differ significantly between the FGF-2 and the control groups at either time point.
  
  
  CONCLUSIONS
  CBCP loaded with FGF-2 resulted in the early promotion of new bone; however, it failed to produce superior bone regeneration in a later stage of healing.
authors.forename: Hyun-Chang
authors.initials: HC
authors.lastname: Lim
authors.forename: Won-Sun
authors.initials: WS
authors.lastname: Baek
authors.forename: Jung-Seok
authors.initials: JS
authors.lastname: Lee
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Choi
authors.forename: Ui-Won
authors.initials: UW
authors.lastname: Jung
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D003094:Collagen / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000627:therapeutic use*; D006882:Hydroxyapatites / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D008297:Male; D008443:Maxillary Sinus / Q000473:pathology / Q000601:surgery; D009297:Nasal Mucosa / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D010865:Pilot Projects; D011817:Rabbits; D059546:Sinus Floor Augmentation / Q000379:methods*; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D006882:Hydroxyapatites; C074950:hydroxyapatite-beta tricalcium phosphate; D016222:Fibroblast Growth Factor 2; D003094:Collagen