title: Thrombophilia Associated with Anti-DFS70 Autoantibodies.
abstract: BACKGROUND
  Anti-DFS70 antibodies are the most frequent antinuclear antibodies (ANA) found in healthy individuals. We assessed the clinical significance of the presence of anti-DFS70 antibodies.
  
  
  METHODS
  We defined a group of patients (n = 421) with anti-DFS70 antibodies and a group of patients (n = 63) with a history of idiopathic arterial and/or venous thrombotic disease and/or obstetric complication (i.e. ≥ 3 miscarriages, fetal death or premature birth with eclampsia). Anti-DFS70 antibodies prevalence was also assessed in a cohort of 300 healthy blood donors.
  
  
  RESULTS
  The prevalence of thrombotic disease and/or obstetric complication in the 421 patients with anti-DFS70 antibodies was 13.1% (n = 55) and the prevalence of connective tissue disease was 19% (n = 80). Among the 63 patients with a history of thrombosis and/or obstetric complications, 7 (11.1%) had anti-DFS70 antibodies and among the latter, 5 had no common thrombophilic factor. In contrast, the prevalence of anti-DFS70 antibodies was of 3.0% (9 out of 300) in healthy donors. Finally, the Activated Partial Thromboplastin Time (aPTT) ratio of patients with a history of thrombosis and anti-DFS70 antibodies was lower than the aPTT ratio of other patients, suggesting that thrombotic patients with anti-DFS70 antibodies may have a hypercoagulable state.
  
  
  CONCLUSIONS
  We described here for the first time an immune procoagulant state involving anti-DFS70 antibodies.
authors.affiliation: Département d'Immunologie, Hôpital Pitié-Salpêtrière (AP-HP), Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Marlet
authors.affiliation: Laboratoire d'Hématologie, Hôpital Pitié-Salpêtrière (AP-HP), Paris, France.
authors.forename: Annick
authors.initials: A
authors.lastname: Ankri
authors.affiliation: Département d'Immunologie, Hôpital Pitié-Salpêtrière (AP-HP), Paris, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Charuel
authors.affiliation: Département d'Immunologie, Hôpital Pitié-Salpêtrière (AP-HP), Paris, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Ghillani-Dalbin
authors.affiliation: Département d'Immunologie, Hôpital Pitié-Salpêtrière (AP-HP), Paris, France.
authors.forename: Amélie
authors.initials: A
authors.lastname: Perret
authors.affiliation: Laboratoire d'Hématologie, Hôpital Pitié-Salpêtrière (AP-HP), Paris, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Martin-Toutain
authors.affiliation: INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses (CIMI), Paris, France; Service de Médecine Interne 2, institut E3M, Centre de référence national du lupus et du syndrome des antiphospholipides, Hôpital Pitié-Salpêtrière (AP-HP), Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Haroche
authors.affiliation: INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses (CIMI), Paris, France; Service de Médecine Interne 2, institut E3M, Centre de référence national du lupus et du syndrome des antiphospholipides, Hôpital Pitié-Salpêtrière (AP-HP), Paris, France.
authors.forename: Zahir
authors.initials: Z
authors.lastname: Amoura
authors.affiliation: Département d'Immunologie, Hôpital Pitié-Salpêtrière (AP-HP), Paris, France.
authors.forename: Lucile
authors.initials: L
authors.lastname: Musset
authors.affiliation: Département d'Immunologie, Hôpital Pitié-Salpêtrière (AP-HP), Paris, France; INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses (CIMI), Paris, France.
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyara
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D015331:Cohort Studies; D003240:Connective Tissue Diseases / Q000453:epidemiology / Q000473:pathology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008297:Male; D008875:Middle Aged; D010314:Partial Thromboplastin Time; D015995:Prevalence; D019851:Thrombophilia / Q000453:epidemiology / Q000473:pathology*; D013927:Thrombosis / Q000473:pathology; D014157:Transcription Factors / Q000276:immunology*; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D001323:Autoantibodies; C504649:PSIP1 protein, human; D014157:Transcription Factors