title: In silico identification of Bell pepper endornavirus from pepper transcriptomes and their phylogenetic and recombination analyses.
abstract: Here, we identified eight Bell pepper endornavirus (BPEV) isolates from nine different pepper transcriptomes. BPEV was present with low copy numbers ranging from 0.01% to 0.18% in the host transcriptome. Phylogenetic identified two different groups of BPEV isolates. Sequence alignment of the five BPEV genomes revealed conservation of the 5' and 3' untranslated regions. Recombination analysis identified two possible recombinant events in the isolate Yolo Wonder. Single nucleotide variation profiles revealed the presence of BPEV variants within a single pepper cultivar. Taken together, this study provides phylogenetic and recombination analyses of the genus Endornavirus using pepper transcriptome data.
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Yeonhwa
authors.initials: Y
authors.lastname: Jo
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Hoseong
authors.initials: H
authors.lastname: Choi
authors.affiliation: Virology Unit, Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, RDA, Wan-Ju 565-852, Republic of Korea.
authors.forename: Ju-Yeon
authors.initials: JY
authors.lastname: Yoon
authors.affiliation: Virology Unit, Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, RDA, Wan-Ju 565-852, Republic of Korea.
authors.forename: Seung-Kook
authors.initials: SK
authors.lastname: Choi
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea. Electronic address: wonkyong@gmail.com.
authors.forename: Won Kyong
authors.initials: WK
authors.lastname: Cho
mesh_terms: D002212:Capsicum / Q000235:genetics / Q000821:virology*; D003198:Computer Simulation; D020869:Gene Expression Profiling / Q000379:methods; D014644:Genetic Variation; D010802:Phylogeny; D010942:Plant Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D018749:RNA, Plant / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D011995:Recombination, Genetic
chemical_list: D018749:RNA, Plant; D012367:RNA, Viral