title: The Effects of Curcumin on Aflatoxin B1- Induced Toxicity in Rats.
abstract: To evaluate the potential of curcumin on toxic and carcinogenic effects of Aflatoxin B1 (AFB1) in relation to AFB1 metabolism, we studied the effects of curcumin on hepatic AFB1-DNA adduct formation and glutathione S-transferase (GST) activity, and the toxic effects of AFB1 in male Fischer 344 rats. Oral administration of curcumin to 5-week-old male rats at a dose of 8 or 80 mg/kg for five consecutive days for three weeks resulted in reduction of AFB1-DNA adduct formation mediated by both liver microsomal and postmitochondrial fractions. The activity of liver GST toward a universal substrate, CDNB, was increased in curcumin-administered rats. As for the acute toxicity of AFB1, curcumin was orally administered to rats for 3 weeks and then AFB1 was given by intragastric intubation. The result showed a decrease of plasma AST and ALT activities in curcumin-treated rats compared with those which received AFB1 alone. Moreover, we have observed that curcumin also reduced glutathione S-transferase placental form positive single cells and foci caused by AFB1 treatment. These results demonstrate the potential of curcumin to reduce the toxic and carcinogenic effects of AFB1 by modulating hepatic drug metabolizing enzymes responsible for AFB1 metabolism.
authors.affiliation: Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University.
authors.forename: Saranya
authors.initials: S
authors.lastname: Poapolathep
authors.forename: Kanjana
authors.initials: K
authors.lastname: Imsilp
authors.forename: Kenji
authors.initials: K
authors.lastname: Machii
authors.forename: Susumu
authors.initials: S
authors.lastname: Kumagai
authors.forename: Amnart
authors.initials: A
authors.lastname: Poapolathep
mesh_terms: D000284:Administration, Oral; D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D003474:Curcumin / Q000008:administration & dosage*; D018736:DNA Adducts / Q000032:analysis; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000008:administration & dosage*; D005982:Glutathione Transferase / Q000032:analysis; D008099:Liver / Q000201:enzymology; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D011041:Poisoning / Q000188:drug therapy*; D011916:Rats, Inbred F344
chemical_list: D002273:Carcinogens; D018736:DNA Adducts; D004791:Enzyme Inhibitors; D009153:Mutagens; D016604:Aflatoxin B1; D005982:Glutathione Transferase; D003474:Curcumin