title: Peroxynitrite induced fibrinogen site identification.
abstract: An increasing number of peroxynitrite-mediated fibrinogen nitrifications have been associated with thrombotic diseases. However, few reports related to priority nitrified fibrinogen injury sites exist. In this paper, an improved method, which simulated the environment in vivo, was used to inspect the structural changes of fibrinogen treated with peroxynitrite and LC-MS/MS in order to investigate the fibrinogen injury sites. The SDS-PAGE results indicated that γ subunits of Fg were vulnerable to oxidative/nitrative modifications induced by peroxynitrite. An in-depth analysis of fibrinogen γ chain (Fg I) nitration site identification and susceptibility to peroxynitrite-utilizing LC-MS/MS strategy was performed. Based on a large dataset, the results indicated the priority injury sites during database searching were Y96, Y262, Y274, Y348, and Y363; these results could be applied to biomedical studies.
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology, 100124 P.R. China.
authors.forename: Yunjing
authors.initials: Y
authors.lastname: Luo
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology, 100124 P.R. China.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Shi
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology, 100124 P.R. China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D030421:Peroxynitrous Acid / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D030421:Peroxynitrous Acid; D005340:Fibrinogen
keywords: Fibrinogen; LC-MS/MS; SDS-PAGE; peroxynitrite; tyrosine nitration