title: Programmable Laser-Assisted Surface Microfabrication on a Poly(Vinyl Alcohol)-Coated Glass Chip with Self-Changing Cell Adhesivity for Heterotypic Cell Patterning.
abstract: Organs are composed of heterotypic cells with patterned architecture that enables intercellular interaction to perform specific functions. In tissue engineering, the ability to pattern heterotypic cells into desired arrangement will allow us to model complex tissues in vitro and to create tissue equivalents for regeneration. This study was aimed at developing a method for fast heterotypic cell patterning with controllable topological manipulation on a glass chip. We found that poly(vinyl alcohol)-coated glass showed a biphasic change in adhesivity to cells in vitro: low adhesivity in the first 24 h and higher adhesivity at later hours due to increased serum protein adsorption. Combining programmable CO2 laser ablation to remove poly(vinyl alcohol) and glass, we were able to create arrays of adhesive microwells of adjustable patterns. We tested whether controllable patterns of epithelial-mesenchymal interaction could be created. When skin dermal papilla cells and fibroblasts were seeded respectively 24 h apart, we were able to pattern these two cells into aggregates of dermal papilla cells in arrays of microwells in a background of fibroblasts sheet. Seeded later, keratinocytes attached to these mesenchymal cells. Keratinocytes contacting dermal papilla cells started to differentiate toward a hair follicle fate, demonstrating patternable epithelial-mesenchymal interaction. This method allows fast adjustable heterotypic cell patterning and surface topology control and can be applied to the investigation of heterotypic cellular interaction and creation of tissue equivalent in vitro.
authors.affiliation: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University , No. 1, Section 1, Jen-Ai Road, Taipei 100, Taiwan.
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Li
authors.affiliation: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University , No. 1, Section 1, Jen-Ai Road, Taipei 100, Taiwan.
authors.forename: Meng-Wei
authors.initials: MW
authors.lastname: Lin
authors.affiliation: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University , No. 1, Section 1, Jen-Ai Road, Taipei 100, Taiwan.|Research Center for Applied Sciences, Academia Sinica , Taipei 115-29, Taiwan.
authors.forename: Meng-Hua
authors.initials: MH
authors.lastname: Yen
authors.affiliation: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University , No. 1, Section 1, Jen-Ai Road, Taipei 100, Taiwan.
authors.forename: Sabrina Mai-Yi
authors.initials: SM
authors.lastname: Fan
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University , Taipei 100, Taiwan.
authors.forename: June-Tai
authors.initials: JT
authors.lastname: Wu
authors.affiliation: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University , No. 1, Section 1, Jen-Ai Road, Taipei 100, Taiwan.
authors.forename: Tai-Horng
authors.initials: TH
authors.lastname: Young
authors.affiliation: Research Center for Applied Sciences, Academia Sinica , Taipei 115-29, Taiwan.
authors.forename: Ji-Yen
authors.initials: JY
authors.lastname: Cheng
authors.affiliation: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University , No. 1, Section 1, Jen-Ai Road, Taipei 100, Taiwan.|Department of Dermatology, National Taiwan University Hospital and College of Medicine , Taipei 100, Taiwan.|Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University , Taipei 100, Taiwan.|Molecular Imaging Center, National Taiwan University , Taipei 100, Taiwan.
authors.forename: Sung-Jan
authors.initials: SJ
authors.lastname: Lin
mesh_terms: D000327:Adsorption; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005898:Glass / Q000737:chemistry*; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D054020:Lasers, Gas; D018613:Microscopy, Confocal; D011143:Polyvinyl Chloride / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties / Q000528:radiation effects
chemical_list: D001798:Blood Proteins; D020099:Coated Materials, Biocompatible; D011143:Polyvinyl Chloride
keywords: dermal papilla; fibroblast; hair follicle; keratinocyte; surface topology; tissue equivalent