title: The proteome of mouse vestibular hair bundles over development.
abstract: Development of the vertebrate hair bundle is a precisely orchestrated event that culminates in production of a tightly ordered arrangement of actin-rich stereocilia and a single axonemal kinocilium. To understand how the protein composition of the bundle changes during development, we isolated bundles from young (postnatal days P4-P6) and mature (P21-P25) mouse utricles using the twist-off method, then characterized their constituent proteins using liquid-chromatography tandem mass spectrometry with data-dependent acquisition. Using MaxQuant and label-free quantitation, we measured relative abundances of proteins in both bundles and in the whole utricle; comparison of protein abundance between the two fractions allows calculation of enrichment in bundles. These data, which are available via ProteomeXchange with identifier PXD002167, will be useful for examining the proteins present in mammalian vestibular bundles and how their concentrations change over development.
authors.affiliation: Oregon Hearing Research Center & Vollum Institute, Oregon Health & Science University , Portland 97239, OR, USA.
authors.forename: Jocelyn F
authors.initials: JF
authors.lastname: Krey
authors.affiliation: W.M. Keck Biomedical Mass Spectrometry Lab, University of Virginia , Charlottesville 22908, VA, USA.
authors.forename: Nicholas E
authors.initials: NE
authors.lastname: Sherman
authors.affiliation: W.M. Keck Biomedical Mass Spectrometry Lab, University of Virginia , Charlottesville 22908, VA, USA.
authors.forename: Erin D
authors.initials: ED
authors.lastname: Jeffery
authors.affiliation: Department of Public Health & Preventive Medicine, Oregon Health & Science University , Portland 97239, OR, USA.
authors.forename: Dongseok
authors.initials: D
authors.lastname: Choi
authors.affiliation: Oregon Hearing Research Center & Vollum Institute, Oregon Health & Science University , Portland 97239, OR, USA.
authors.forename: Peter G
authors.identifier: 0000-0002-9787-5860
authors.initials: PG
authors.lastname: Barr-Gillespie
mesh_terms: D000818:Animals; D018069:Hair Cells, Vestibular / Q000378:metabolism; D051379:Mice; D020543:Proteome; D053719:Tandem Mass Spectrometry; D014722:Vestibule, Labyrinth / Q000254:growth & development / Q000378:metabolism
chemical_list: D020543:Proteome