title: Anti-hypernociceptive and anti-oxidative effects of locally treated dobutamine in diabetic rats.
abstract: BACKGROUND
  Oxidative stress as a significant factor in the development of diabetes induced neuropathic pain as well as the potential for prevention of this complication. Therefore, we hypothesized that locally administrated dobutamine, a beta-adrenoreceptor agonist, or esmolol, a beta-adrenoreceptor antagonist, can modulate the oxidative stress and ameliorate the diabetes induced neuropathic pain.
  
  
  METHODS
  Effects of locally (intraplantar) treated two pharmaceutical preparations used in clinical applications, dobutamine or esmolol, were investigated by measuring thermal latencies, mechanical thresholds and several oxidative stress parameters in streptozotocin (STZ) induced diabetic rats.
  
  
  RESULTS
  Diabetes induced hyperalgesia and allodynia more effectively relieved by dobutamine than esmolol. Anti-hypersensitive action of dobutamine continued through the experiment. Diabetes induced oxidative damage in the paw tissues since STZ rats showed significant increased malondialdehyde (MDA), nitric oxide (NO) and decreased superoxide dismutase (SOD), catalase (CAT), myeloperoxidase (MPO) in the paw. Dobutamine, but not esmolol, restored the tissue oxidative and nitrossive stress parameters to those observed in the non-diabetic rats.
  
  
  CONCLUSIONS
  Findings suggest that diabetes-induced oxidative stress may be partially responsible for the development of diabetic neural complications. Amelioration of oxidative stress by locally treated dobutamine can be beneficial in diabetes induced neuropathic pain.
authors.affiliation: Department of Biophysics, School of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey. Electronic address: tufanmert@yahoo.com.
authors.forename: Tufan
authors.initials: T
authors.lastname: Mert
authors.affiliation: Anaesthesiology and Reanimation, School of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey.
authors.forename: Hafize
authors.initials: H
authors.lastname: Oksuz
authors.affiliation: Departments of Anatomy, School of Medicine, Fatih University, Istanbul, Turkey.
authors.forename: Berin
authors.initials: B
authors.lastname: Tugtag
authors.affiliation: Biochemistry, School of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey.
authors.forename: Metin
authors.initials: M
authors.lastname: Kilinc
authors.affiliation: Biochemistry, School of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Sahin
authors.affiliation: Neurosurgery, School of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey.
authors.forename: Idiris
authors.initials: I
authors.lastname: Altun
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D004280:Dobutamine / Q000494:pharmacology*; D005260:Female; D006358:Hot Temperature; D006930:Hyperalgesia / Q000188:drug therapy*; D018384:Oxidative Stress / Q000187:drug effects; D010147:Pain Measurement / Q000187:drug effects; D011412:Propanolamines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D000700:Analgesics; D000975:Antioxidants; D011412:Propanolamines; D004280:Dobutamine; C036604:esmolol
keywords: Diabetic Neuropathy; Dobutamine; Esmolol; Oxidative stress; Rat