title: CORRELATION BETWEEN CLINICAL PATHOLOGY OF LUMINAL B BREAST CANCER AND DETERMINATION OF ESTROGEN RECEPTOR, PROGESTERONE RECEPTOR AND HER2 EXPRESSION COMBINED WITH NUCLEAR MORPHOLOGY.
abstract: Breast cancer, one of the most common malignant tumors in females, draws little attention because of its untypical symptoms and signs, so the disease is usually confirmed too late, in an advanced stage. Based on the detection of nuclear morphology parameters of luminal B breast cancer, this study explored how pathological features relate to estrogen receptor (ER) and progesterone receptor (PR) expression of human epidermal growth factor receptor-2 (HER2). A quantity of 354 breast cancer specimens with follow-up records from the department of pathology in the First Peoples Hospital of Nantong and the Tumor Hospital of Nantong were selected as research subjects. Nuclear parameters of specimens stained by hematoxylin and eosin were measured by imaging analysis software. It was found that breast cancer can be divided into four types, luminal B, luminal A, HER2 over-expression and basal-like type based on immunohistochemical results of three antibodies, i.e, ER, PR and HER2. A total of 113 patients (31.8%) were confirmed with luminal B breast cancer, mostly in histological stage II; the difference of nuclear morphology was of statistical significance between ER+/PR+ and ER-/PR- (P<0.05), and most ER-/PR- was histologically confirmed as stage III, with lower survival rate than ER+/PR+ (P<0.05). Among these four subtypes of breast cancer, luminal B had the lowest brain metastasis rate, while HER2 over-expression subtype was found with the highest rate of lung and pleura metastasis. Besides, luminal B possessed longer disease-free survival (DFS) than basal-like (P<0.05) and longer total survival (OS) than HER2 over-expression (P<0.05) and basal-like subtypes (P<0.05). It can be concluded that detection of ER, PR and HER2 in combination with nuclear morphology is beneficial to evaluate treatment and prognosis of breast cancer.
authors.affiliation: Oncology Department, The First Peoples Hospital of Nantong, Nantong, Jiangsu, China.
authors.forename: D
authors.initials: D
authors.lastname: Yin
authors.affiliation: Oncology Department, The First Peoples Hospital of Nantong, Nantong, Jiangsu, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Oncology Department, The First Peoples Hospital of Nantong, Nantong, Jiangsu, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Oncology Department, The First Peoples Hospital of Nantong, Nantong, Jiangsu, China.
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.affiliation: Oncology Department, The First Peoples Hospital of Nantong, Nantong, Jiangsu, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: General Surgery Department, The First Peoples Hospital of Nantong, Nantong, Jiangsu, China.
authors.forename: C
authors.initials: C
authors.lastname: Tang
authors.affiliation: Test Section, The First Peoples Hospital of Nantong, Nantong, Jiangsu, China.
authors.forename: W
authors.initials: W
authors.lastname: Xie
authors.affiliation: Key Laboratory of Experimental Animal and Safety Evaluation, Zhejiang Academy of Medical Sciences, Hangzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018572:Disease-Free Survival; D047628:Estrogen Receptor alpha / Q000096:biosynthesis*; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis*; D011980:Receptors, Progesterone / Q000096:biosynthesis*; D015996:Survival Rate
chemical_list: D047628:Estrogen Receptor alpha; D018719:Erb-b2 Receptor Tyrosine Kinases; D011980:Receptors, Progesterone; C508053:ERBB2 protein, human