title: Cryptosporidium identification in HIV-Infected Humans. Experience from Kinshasa, the Democratic Republic of Congo.
abstract: Cryptosporidium is an important protozoan parasite affecting HIV/AIDS patients. To determine the prevalence and the species of Cryptosporidium spp by developing a rapid and efficient real-time PCR-RFLP test. A cross-sectional study was conducted among HIV-infected adults from Kinshasa, the Democratic Republic of Congo. Stool specimens were examined by microscopic evaluation and real-time PCR-RFLP. Out of 242 HIV-infected adults, 10 (4.1%) cases of Cryptosporidium were identified by microscopic examination. Using PCR-RFLP, the prevalence of Cryptosporidium spp was 5.4% (n = 13). All the 13 cases of Cryptosporidium spp had the stage of AIDS of HIV infection. Extracted DNA was amplified by nested PCR targeting a 1030-bp fragment of the 18s RNA gene. RFLP analysis identified one C. parvum, four C. hominis and one non determined Cryptosporidium. The capacity to detect C. parvum, C. hominis and non-determined Cryptosporidium was present among our HIV-infected patients.
authors.forename: Roger D
authors.initials: RD
authors.lastname: Wumba
authors.forename: Josué
authors.initials: J
authors.lastname: Zanga
authors.forename: Kennedy M
authors.initials: KM
authors.lastname: Mbanzulu
authors.forename: Madone N
authors.initials: MN
authors.lastname: Mandina
authors.forename: Aimé K
authors.initials: AK
authors.lastname: Kahindo
authors.forename: Michel N
authors.initials: MN
authors.lastname: Aloni
authors.forename: Mathilde B
authors.initials: MB
authors.lastname: Ekila
mesh_terms: D003430:Cross-Sectional Studies; D003457:Cryptosporidiosis / Q000175:diagnosis* / Q000453:epidemiology* / Q000469:parasitology; D003458:Cryptosporidium / Q000145:classification* / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D015023:Democratic Republic of the Congo / Q000453:epidemiology; D005243:Feces / Q000469:parasitology; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D008853:Microscopy; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D012337:RNA, Ribosomal, 18S / Q000235:genetics
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S