title: Anti-inflammation and anti-apoptosis effects of pearl extract gel on UVB irradiation HaCaT cells.
abstract: Caused by acute radiation skin reaction and injury, receiving radiotherapy treatment process is often performed side-effects on cancer patients. The clinical manifestations of skin irritation, itching, peeling, pigmentation, ulcer bleeding and other symptoms, in addition to causing patient discomfort and affecting quality of life, may increase the risk of local or systemic infection, and lead to interruption of radiation therapy. At present, for acute radiation dermatitis, there is no uniform treatment, and the various methods are evaluated variously. In this study, the authors focus on broken pearls using room temperature super extraction system, the water extraction process of wet-grinding method, nano-scale pearl, along with a large number of high purity natural amino acid extracts in the water. The room-temperature super-extraction system (RTSES) can be extracted from a relatively high-volume of pearl extract. We use pearl extract as the main component of experimental material, and the blending of pearl extract and poly (γ-glutamic acid) is used to form biodegradable composite hydrogels. This study aims to evaluate the use of RTSES to extract the major active components of pearl and enhance their anti-inflammation and anti-apoptosis effects. The possible effect of pearl extract on inducing apoptosis in human keratinocyte cells (HaCaT) under the exposure of low dose UVB has been investigated. Various concentrations of pearl extracts have been used to study the effect of low dosage UVB on HaCaT cells. The results show that pearl extract has no toxic effect on HaCaT cells. Combining the pearl extract and poly (γ-glutamic acid) hydrogels with UVB irradiation would decrease the inflammation and apoptosis of HaCaT cells. The commercial pearl extract has the potential to inhibit radiation dermatitis occurring within keratinocyte cells.
authors.affiliation: Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Keelung, Taiwan.
authors.forename: Yueh-Lung
authors.initials: YL
authors.lastname: Yang
authors.affiliation: Department of Chinese Medicine, En Chu Kong Hospital, New Taipei City, Taiwan.|Department of Cosmetic Science, Chang Gung University of Science and Technology, Taoyuan, Taiwan.
authors.forename: Chin-Hsien
authors.initials: CH
authors.lastname: Chang
authors.affiliation: Department of Biomedical Engineering, Ming-Chuan University, Taoyuan, Taiwan.
authors.forename: Ching-Cheng
authors.initials: CC
authors.lastname: Huang
authors.affiliation: Department of Life Science, Fu Jen Catholic University, New Taipei City, Taiwan.
authors.forename: Hsia-Wei
authors.initials: HW
authors.lastname: Liu
mesh_terms: D060105:Animal Shells / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D005782:Gels / Q000737:chemistry; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects*; D008974:Mollusca / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014020:Tissue Extracts / Q000008:administration & dosage*; D016896:Treatment Outcome; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D000893:Anti-Inflammatory Agents; D005782:Gels; D017382:Reactive Oxygen Species; D014020:Tissue Extracts
keywords: UVB irradiation; anti-apoptosis; anti-inflammation; pearl extract; poly (γ-glutamic acid)