title: Resveratrol inhibits the hydrogen dioxide-induced apoptosis via Sirt 1 activation in osteoblast cells.
abstract: Sirt 1 plays a critical role in stress responses. We determined the deregulation of Sirt 1 activity, p53 acetylation, Bcl-2 expression, and mitochondria-dependent apoptosis in mouse osteoblast MC3T3-E1 cells which were exposed to H2O2. And then we investigated the protective role of Sirt 1 activator, Resveratrol (RSV), against the H2O2-induced apoptosis. Results demonstrated that Sirt 1 and Bcl-2 were inhibited, whereas p53 acetylation, Bax, and caspase 9 were promoted by H2O2, as was aggravated by the Sirt 1 inhibitor, EX-527. Instead, RSV inhibited the H2O2-induced both p53 acetylation and the caspase 9 activation, whereas ameliorated the H2O2-induced Bcl-2 inhibition and apoptosis. In conclusion, Sirt 1 was downregulated during the H2O2-induced apoptosis in MC3T3-E1 cells. And the chemical activation of Sirt 1 inhibited the H2O2-induced apoptosis via the downregulation of p53 acetylation. Our results suggest that Sirt 1 upregulation appears to be an important strategy to inhibit the oxidative stress-induced apoptosis.
authors.affiliation: a Department of Anatomy College of Basic Medicine , Beihua University , Jilin City , China.
authors.forename: Na
authors.initials: N
authors.lastname: He
authors.affiliation: b The Otolaryngological Department , China-Japan Union Hospital of Jilin University , Changchun , China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Zhu
authors.affiliation: c Department of Neurology , Xuanwu Hospital of Capital Medical University , Beijing , China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.affiliation: d Department of Hand Surgery , Affiliated Hospital of Beihua University , Jilin City , China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Zhao
authors.affiliation: d Department of Hand Surgery , Affiliated Hospital of Beihua University , Jilin City , China.
authors.forename: Weiyan
authors.initials: W
authors.lastname: Zhao
authors.affiliation: d Department of Hand Surgery , Affiliated Hospital of Beihua University , Jilin City , China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000107:Acetylation; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000633:toxicity; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects; D056564:Sirtuin 1 / Q000096:biosynthesis* / Q000235:genetics; D013267:Stilbenes / Q000008:administration & dosage*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D013267:Stilbenes; D016159:Tumor Suppressor Protein p53; D006861:Hydrogen Peroxide; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1; D000077185:Resveratrol
keywords: MC3T3-E1 osteoblast cells; apoptosis; oxidative stress; sirt 1