title: Release kinetics of an amphiphilic photosensitizer by block-polymer nanoparticles.
abstract: Block-polymer nanoparticles are now well-known candidates for the delivery of various non-soluble drugs to cells. The release of drugs from these nanoparticles is a major concern related to their efficiency as nanovectors and is still not completely deciphered. Various processes have been identified, depending of both the nature of the block-polymer and those of the drugs used. We focused our interest on an amphiphilic photosensitizer studied for photodynamic treatments of cancer, Pheophorbide-a (Pheo). We studied the transfer of Pheo from poly(ethyleneglycol-b-ϵ-caprolactone) nanoparticles (I) to MCF-7 cancer cells and (II) to models of membranes. Altogether, our results suggest that the delivery of the major part of the Pheo by the nanoparticles occurs via a direct transfer of Pheo from the nanoparticles to the membrane, by collision. A minor process may involve the internalization of a small amount of the nanoplatforms by the cells. So, this research illustrates the great care necessary to address the question of the choice of such nanocarriers, in relation with the properties - in particular the relative hydrophobicity - of the drugs encapsulated, and gives elements to predict the mechanism and the efficiency of the delivery.
authors.affiliation: Université Pierre et Marie Curie-Paris 6, Laboratoire Jean Perrin - UMR8237, 4 place Jussieu, Paris F-75005, France; CNRS, Laboratoire Jean Perrin, UMR8237, 4 place Jussieu, Paris F-75005, France.
authors.forename: Rachid
authors.initials: R
authors.lastname: Kerdous
authors.affiliation: Université Pierre et Marie Curie-Paris 6, Laboratoire Jean Perrin - UMR8237, 4 place Jussieu, Paris F-75005, France; CNRS, Laboratoire Jean Perrin, UMR8237, 4 place Jussieu, Paris F-75005, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Sureau
authors.affiliation: Université Pierre et Marie Curie-Paris 6, Laboratoire Jean Perrin - UMR8237, 4 place Jussieu, Paris F-75005, France; CNRS, Laboratoire Jean Perrin, UMR8237, 4 place Jussieu, Paris F-75005, France.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Bour
authors.affiliation: Université Pierre et Marie Curie-Paris 6, Laboratoire Jean Perrin - UMR8237, 4 place Jussieu, Paris F-75005, France; CNRS, Laboratoire Jean Perrin, UMR8237, 4 place Jussieu, Paris F-75005, France. Electronic address: stephanie.bonneau@upmc.fr.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Bonneau
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods; D002734:Chlorophyll / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D065546:Drug Liberation; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007783:Lactones / Q000737:chemistry; D061986:MCF-7 Cells; D053758:Nanoparticles; D011092:Polyethylene Glycols / Q000737:chemistry; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D012995:Solubility
chemical_list: D004337:Drug Carriers; D007783:Lactones; D011838:Radiation-Sensitizing Agents; C521904:poly(ethylene glycol)-block-poly(epsilon-caprolactone); D002734:Chlorophyll; D011092:Polyethylene Glycols; C032623:pheophorbide a
keywords: Block-polymer nanoparticles; Collisions; Drug release; Photosensitizer; Transfer