title: Melanin is required for the formation of the multi-cellular conidia in the endophytic fungus Pestalotiopsis microspora.
abstract: Melanin plays an important role in regulating various biological processes in many fungi. However, its biological role in conidiation remains largely elusive. We report here that conidia production, morphogenesis, integrity, germination and their viability in Pestalotiopsis microspora require the polyketide-derived melanin. A polyketide synthase gene, pks1, was identified and demonstrated responsible for melanin biosynthesis in this fungus. A targeted deletion mutant strain Δpks1 displayed a defect in pigmentation of conidia and had an albino colonial phenotype. Interestingly, Δpks1 produced approximately 6-fold as many conidia as the wild type did, suggesting a negative modulation of melanin on conidia production in this fungus. Moreover, the conidia failed to develop into the normal five-cell morphology, rather the three main-body cells separated via constriction at the original septum position to generate three independent mutant conidia. This result suggests a novel role of melanin in the formation of the multi-cellular conidia. Germ tubes could develop from the three different types of mutant conidia and kept elongating, despite a significantly lower germination rate was observed for them. Still more, the unpigmented conidia became permeable to Calcofluor White and DAPI, suggesting the integrity of the conidia was impaired. Deliberate inhibition of melanin biosynthesis by a specific inhibitor, tricyclazole, led to a similar phenotypes. This work demonstrates a new function of fungal melanin in conidial development.
authors.affiliation: State Key Program of Microbiology and Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 3 00071, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Yu
authors.affiliation: Institute of Biochemistry and Molecular Biology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Huo
authors.affiliation: State Key Program of Microbiology and Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 3 00071, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Program of Microbiology and Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 3 00071, China.
authors.forename: Longfei
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Program of Microbiology and Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 3 00071, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Program of Microbiology and Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 3 00071, China; Institute of Biochemistry and Molecular Biology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China. Electronic address: xudong82@nankai.edu.cn.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhu
mesh_terms: D060054:Agrobacterium / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D018525:Germination; D008543:Melanins / Q000096:biosynthesis* / Q000378:metabolism; D050296:Microbial Viability; D009024:Morphogenesis; D010802:Phylogeny; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism; D013172:Spores, Fungal / Q000254:growth & development / Q000502:physiology*; D013098:Xylariales / Q000502:physiology
chemical_list: D005656:Fungal Proteins; D008543:Melanins; D048630:Polyketide Synthases
keywords: Conidial development; Germination tube; Melanin; Pestalotiopsis microspora; Polyketide synthase