title: Subangstrom single-molecule measurements of motor proteins using a nanopore.
abstract: Techniques for measuring the motion of single motor proteins, such as FRET and optical tweezers, are limited to a resolution of ∼300 pm. We use ion current modulation through the protein nanopore MspA to observe translocation of helicase Hel308 on DNA with up to ∼40 pm sensitivity. This approach should be applicable to any protein that translocates on DNA or RNA, including helicases, polymerases, recombinases and DNA repair enzymes.
authors.affiliation: Department of Physics, University of Washington, Seattle, Washington, USA.
authors.forename: Ian M
authors.initials: IM
authors.lastname: Derrington
authors.affiliation: Department of Physics, University of Washington, Seattle, Washington, USA.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Craig
authors.affiliation: Illumina Inc., San Diego, California, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Stava
authors.affiliation: Department of Physics, University of Washington, Seattle, Washington, USA.
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Laszlo
authors.affiliation: Department of Physics, University of Washington, Seattle, Washington, USA.
authors.forename: Brian C
authors.initials: BC
authors.lastname: Ross
authors.affiliation: Department of Physics, University of Washington, Seattle, Washington, USA.
authors.forename: Henry
authors.identifier: http://orcid.org/0000-0003-2735-8048
authors.initials: H
authors.lastname: Brinkerhoff
authors.affiliation: Department of Physics, University of Washington, Seattle, Washington, USA.
authors.forename: Ian C
authors.initials: IC
authors.lastname: Nova
authors.affiliation: Department of Physics, University of Washington, Seattle, Washington, USA.
authors.forename: Kenji
authors.initials: K
authors.lastname: Doering
authors.affiliation: Department of Physics, University of Washington, Seattle, Washington, USA.
authors.forename: Benjamin I
authors.initials: BI
authors.lastname: Tickman
authors.affiliation: Illumina Inc., San Diego, California, USA.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Ronaghi
authors.affiliation: Illumina Inc., San Diego, California, USA.
authors.forename: Jeffrey G
authors.initials: JG
authors.lastname: Mandell
authors.affiliation: Illumina Inc., San Diego, California, USA.
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Gunderson
authors.affiliation: Department of Physics, University of Washington, Seattle, Washington, USA.
authors.forename: Jens H
authors.initials: JH
authors.lastname: Gundlach
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D004265:DNA Helicases / Q000737:chemistry* / Q000648:ultrastructure; D055119:Elastic Modulus; D008422:Materials Testing / Q000379:methods; D008846:Micromanipulation / Q000379:methods*; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000648:ultrastructure; D009038:Motion; D058608:Nanopores / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods; D011485:Protein Binding; D013314:Stress, Mechanical
chemical_list: D020409:Molecular Motor Proteins; D004247:DNA; D004265:DNA Helicases