title: Identification of Dp71 Isoforms Expressed in PC12 Cells: Subcellular Localization and Colocalization with β-Dystroglycan and α1-Syntrophin.
abstract: Several dystrophin Dp71 messenger RNA (mRNA) alternative splice variants have been described. According to the splicing of exon 78 or intron 77, Dp71 proteins are grouped as Dp71d, Dp71f, and Dp71e, and each group has a specific C-terminal end. In this study, we explored the expression of Dp71 isoforms at the complementary DNA (cDNA) level and the subcellular localization of recombinant Myc-Dp71 proteins in PC12 cells. We determined that PC12 cells express Dp71a, Dp71c, Dp71ab, Dp71e, and Dp71ec mRNA splice variants. In undifferentiated and nerve growth factor-differentiated PC12 Tet-ON cells, Dp71a, Dp71ab, and Dp71e were found to localize and colocalize with β-dystroglycan and α1-syntrophin in the periphery/cytoplasm, while Dp71c and Dp71ec were mainly localized in the cell periphery and showed less colocalization with β-dystroglycan and α1-syntrophin. The levels of Dp71a, Dp71e, and Dp71ec were increased in the nucleus of differentiated PC12 Tet-ON cells compared to undifferentiated cells. Dp71 isoforms were also localized in neurite extensions and growth cones.
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN No. 2508, San Pedro Zacatenco, C.P. 07360, México, D. F., Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Aragón
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN No. 2508, San Pedro Zacatenco, C.P. 07360, México, D. F., Mexico.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Martínez-Herrera
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN No. 2508, San Pedro Zacatenco, C.P. 07360, México, D. F., Mexico.|Departamento de Genómica Humana, Instituto Nacional de Perinatología, C.P. 11000, México, D.F., Mexico.
authors.forename: José
authors.initials: J
authors.lastname: Romo-Yáñez
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN No. 2508, San Pedro Zacatenco, C.P. 07360, México, D. F., Mexico.
authors.forename: Víctor
authors.initials: V
authors.lastname: Ceja
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN No. 2508, San Pedro Zacatenco, C.P. 07360, México, D. F., Mexico.
authors.forename: Coztli
authors.initials: C
authors.lastname: Azotla-Vilchis
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN No. 2508, San Pedro Zacatenco, C.P. 07360, México, D. F., Mexico.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Siqueiros-Márquez
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN No. 2508, San Pedro Zacatenco, C.P. 07360, México, D. F., Mexico.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Soid-Raggi
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN No. 2508, San Pedro Zacatenco, C.P. 07360, México, D. F., Mexico.|Departamento de Infectología e Inmunología, Instituto Nacional de Perinatología, C.P. 11000, México, D.F., Mexico.
authors.forename: Alma
authors.initials: A
authors.lastname: Herrera-Salazar
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN No. 2508, San Pedro Zacatenco, C.P. 07360, México, D. F., Mexico. cecim@cinvestav.mx.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Montañez
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D049030:Dystroglycans / Q000235:genetics / Q000378:metabolism*; D016189:Dystrophin / Q000235:genetics / Q000378:metabolism; D020439:Growth Cones / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D016716:PC12 Cells; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D051381:Rats
chemical_list: D002135:Calcium-Binding Proteins; D016189:Dystrophin; D008565:Membrane Proteins; D009124:Muscle Proteins; D020033:Protein Isoforms; C085339:apo-dystrophin 1; C091825:syntrophin alpha1; D049030:Dystroglycans
keywords: Dp71 isoforms; PC12 Cells; Subcellular localization; α1-Syntrophin; β-Dystroglycan