title: Laser Photocoagulation Induces Transduction of Retinal Pigment Epithelial Cells by Intravitreally Administered Adeno-Associated Viral Vectors.
abstract: Retinal transduction by intravitreally administered adeno-associated viral (AAV) vector is previously known to be extremely limited to the neural retina except AAV2 capsid type. Recently, we showed that prior laser photocoagulation enhances retinal transduction of intravitreally administered AAV vectors, including the outer retina and retinal pigment epithelium (RPE). Here, by performing short-pulse laser pretreatment on the mouse retina, we demonstrate RPE cells transduced by three different capsid types of AAV vectors, AAV2, AAV5, and AAV8, using RPE wholemounts. For all capsid types, laser pretreatment effectively induced the transduction of RPE cells in and around the laser site.
authors.affiliation: 1 Department of Ophthalmology, Severance Hospital, Institute of Vision Research, Yonsei University College of Medicine , Seoul, South Korea.
authors.forename: Si Hyung
authors.initials: SH
authors.lastname: Lee
authors.affiliation: 2 Department of Ophthalmology, Soonchunhyang University College of Medicine , Bucheon Hospital, Bucheon, South Korea.
authors.forename: Yoon Jin
authors.initials: YJ
authors.lastname: Kong
authors.affiliation: 3 Myung-Gok Eye Research Institute, Department of Medical Science, Konyang University , Daejeon, Korea.
authors.forename: Jungmook
authors.initials: J
authors.lastname: Lyu
authors.affiliation: 4 Department of Microbiology, Bio-Medical Institute of Technology, Cellular Dysfunction Research Center, University of Ulsan College of Medicine , Seoul, South Korea.
authors.forename: Heuiran
authors.initials: H
authors.lastname: Lee
authors.affiliation: 5 Department of Biopharmacy, Chungbuk Health and Science University , Chungbuk, South Korea.
authors.forename: Keerang
authors.initials: K
authors.lastname: Park
authors.affiliation: 2 Department of Ophthalmology, Soonchunhyang University College of Medicine , Bucheon Hospital, Bucheon, South Korea.|6 Laboratory for Translational Research on Retinal and Macular Degeneration, Soonchunhyang University College of Medicine , Bucheon Hospital, Bucheon, South Korea .
authors.forename: Tae Kwann
authors.initials: TK
authors.lastname: Park
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics*; D000229:Dependovirus / Q000235:genetics*; D005822:Genetic Vectors / Q000008:administration & dosage*; D017075:Laser Coagulation; D051379:Mice; D020419:Photoreceptor Cells, Vertebrate / Q000502:physiology; D012164:Retinal Diseases / Q000235:genetics* / Q000628:therapy*; D055351:Retinal Neurons / Q000502:physiology; D055213:Retinal Pigment Epithelium / Q000502:physiology*; D014161:Transduction, Genetic; D014785:Vision, Ocular
chemical_list: D036022:Capsid Proteins