title: Characterization of DDR2 Inhibitors for the Treatment of DDR2 Mutated Nonsmall Cell Lung Cancer.
abstract: Despite advances in precision medicine approaches over the past decade, the majority of nonsmall cell lung cancers (NSCLCs) are refractory to treatment with targeted small molecule inhibitors. Previous work has identified mutations in the Discoidin Domain Receptor 2 (DDR2) kinase as potential therapeutic targets in NSCLCs. While DDR2 is potently targeted by several multitargeted kinase inhibitors, most notably dasatinib, toxicity has limited the clinical application of anti-DDR2 therapy. Here, we have characterized compound 1 and other tool compounds demonstrating selectivity for DDR2 and show that while these compounds inhibit DDR2 in lung cancer model systems, they display limited antiproliferative activity in DDR2 mutated cell lines as compared to dual DDR2/SRC inhibitors. We show that DDR2 and SRC are binding partners, that SRC activity is tied to DDR2 activation, and that dual inhibition of both DDR2 and SRC leads to enhanced suppression of DDR2 mutated lung cancer cell lines. These results support the further evaluation of dual SRC/DDR2 targeting in NSCLC, and we report a tool compound, compound 5, which potently inhibits both SRC and DDR2 with a distinct selectivity profile as compared to dasatinib.
authors.affiliation: Department of Medical Oncology, Dana-Farber Cancer Institute , Boston, Massachusetts, United States.
authors.forename: Hideki
authors.initials: H
authors.lastname: Terai
authors.affiliation: Department of Cancer Biology, Dana-Farber Cancer Institute , Boston, Massachusetts, United States.|Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School , Boston, Massachusetts, United States.
authors.forename: Li
authors.initials: L
authors.lastname: Tan
authors.affiliation: Department of Medical Oncology, Dana-Farber Cancer Institute , Boston, Massachusetts, United States.
authors.forename: Ellen M
authors.initials: EM
authors.lastname: Beauchamp
authors.affiliation: Department of Cancer Biology, Dana-Farber Cancer Institute , Boston, Massachusetts, United States.|Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School , Boston, Massachusetts, United States.
authors.forename: John M
authors.initials: JM
authors.lastname: Hatcher
authors.affiliation: Department of Cancer Biology, Dana-Farber Cancer Institute , Boston, Massachusetts, United States.|Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School , Boston, Massachusetts, United States.
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Medical Oncology, Dana-Farber Cancer Institute , Boston, Massachusetts, United States.|Department of Pathology, Brigham and Women's Hospital , Boston Massachusetts, United States.|Cancer Program, Broad Institute of Harvard and MIT , Cambridge, Massachusetts, United States.
authors.forename: Matthew
authors.initials: M
authors.lastname: Meyerson
authors.affiliation: Department of Cancer Biology, Dana-Farber Cancer Institute , Boston, Massachusetts, United States.|Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School , Boston, Massachusetts, United States.
authors.forename: Nathanael S
authors.initials: NS
authors.lastname: Gray
authors.affiliation: Department of Medical Oncology, Dana-Farber Cancer Institute , Boston, Massachusetts, United States.|Cancer Program, Broad Institute of Harvard and MIT , Cambridge, Massachusetts, United States.
authors.forename: Peter S
authors.initials: PS
authors.lastname: Hammerman
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D000070818:Discoidin Domain Receptors; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D015151:Immunoblotting; D020128:Inhibitory Concentration 50; D008175:Lung Neoplasms / Q000188:drug therapy*; D015394:Molecular Structure; D009154:Mutation; D020794:Receptor Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors* / Q000235:genetics; D011975:Receptors, Mitogen / Q000037:antagonists & inhibitors* / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D004791:Enzyme Inhibitors; D011975:Receptors, Mitogen; D000070818:Discoidin Domain Receptors; D020794:Receptor Protein-Tyrosine Kinases