title: Interferon gamma response to Mycobacterium avium subsp. paratuberculosis specific lipopentapeptide antigen L5P in cattle.
abstract: After Mycobacterium avium subsp. paratuberculosis (Map) infection the cell-mediated immune (CMI) response indicative of early Th1 activation may be detected using interferon-gamma release assay (IGRA). Currently, the purified protein derivatives (PPDs), i.e., the total extract of mycobacteria antigens are used to recall CMI responses against Map. This study aimed to assess the ability of the chemically synthesized Map specific cell wall lipopentapeptide L5P to induce CMI response in cows infected by Map compared to PPD. L5P and PPD elicited an IFN-γ response in 12 and 35 animals from two Map infected herds respectively, but IFN-γ was not detected in the 13 cows recruited from a non-infected herd. Levels of IFN-γ detected were higher with PPD than with L5P. There was no correlation between the IFN-γ response and the humoral response to Map or faecal culture.
authors.affiliation: UMR1282, Infectiologie et Santé Publique (ISP-311), INRA Centre Val de Loire, F-37380 Nouzilly, France. Electronic address: sebastien.holbert@tours.inra.fr.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Holbert
authors.affiliation: UMR1282, Infectiologie et Santé Publique (ISP-311), INRA Centre Val de Loire, F-37380 Nouzilly, France. Electronic address: maxime.branger@tours.inra.fr.
authors.forename: Maxime
authors.initials: M
authors.lastname: Branger
authors.affiliation: UMR1282, Infectiologie et Santé Publique (ISP-311), INRA Centre Val de Loire, F-37380 Nouzilly, France. Electronic address: a-souriau@orange.fr.
authors.forename: Armel
authors.initials: A
authors.lastname: Souriau
authors.affiliation: Groupement de Défense Sanitaire de la Région Centre (GDS Centre), 4 rue Robert Mallet Stevens BP 501, F-36018 Châteauroux, France. Electronic address: blamoureux@gdma36.fr.
authors.forename: Bérénice
authors.initials: B
authors.lastname: Lamoureux
authors.affiliation: Institut Pasteur, Unité de Chimie des Biomolécules, Département de Biologie Structurale et Chimie, 75015 Paris, France; CNRS UMR 3523, 75015 Paris, France. Electronic address: christelle.ganneau@pasteur.fr.
authors.forename: Christelle
authors.initials: C
authors.lastname: Ganneau
authors.affiliation: UMR1282, Infectiologie et Santé Publique (ISP-311), INRA Centre Val de Loire, F-37380 Nouzilly, France. Electronic address: gaelle703@gmail.com.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Richard
authors.affiliation: UMR1282, Infectiologie et Santé Publique (ISP-311), INRA Centre Val de Loire, F-37380 Nouzilly, France. Electronic address: thierry.cochard@tours.inra.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Cochard
authors.affiliation: Groupement de Défense Sanitaire de la Région Centre (GDS Centre), 4 rue Robert Mallet Stevens BP 501, F-36018 Châteauroux, France. Electronic address: ctholoniat@gdma36.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Tholoniat
authors.affiliation: Institut Pasteur, Unité de Chimie des Biomolécules, Département de Biologie Structurale et Chimie, 75015 Paris, France; CNRS UMR 3523, 75015 Paris, France. Electronic address: sylvie.bay@pasteur.fr.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bay
authors.affiliation: UMR1282, Infectiologie et Santé Publique (ISP-311), INRA Centre Val de Loire, F-37380 Nouzilly, France. Electronic address: nathalie.winter@tours.inra.fr.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Winter
authors.affiliation: Laboratoire Départemental d'Analyse et de Recherche de Dordogne, 161 av Winston Churchill, 24660 Coulounieix Chamiers, France. Electronic address: jl.moyen@dordogne.fr.
authors.forename: Jean Louis
authors.initials: JL
authors.lastname: Moyen
authors.affiliation: UMR1282, Infectiologie et Santé Publique (ISP-311), INRA Centre Val de Loire, F-37380 Nouzilly, France. Electronic address: franck.biet@tours.inra.fr.
authors.forename: Franck
authors.initials: F
authors.lastname: Biet
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D005260:Female; D007111:Immunity, Cellular / Q000276:immunology; D007371:Interferon-gamma / Q000378:metabolism*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology / Q000378:metabolism*; D010283:Paratuberculosis / Q000276:immunology* / Q000382:microbiology; D014373:Tuberculin
chemical_list: D000942:Antigens, Bacterial; D014373:Tuberculin; D007371:Interferon-gamma
keywords: Antigen; Cell mediated immune response; IFN-γ release assay: IGRA; Johne's disease; Lipopentapeptide: L5P; Lipopeptide; Mycobacterium avium subsp. paratuberculosis; Paratuberculosis