title: Paenibacillus faecis sp. nov., isolated from human faeces.
abstract: A spore-forming, rod-shaped Gram-strain-positive bacterium, strain 656.84T, was isolated from human faeces in 1984. It contained anteiso-C15 : 0 as the major cellular fatty acid, meso-diaminopimelic acid was found in the cell wall peptidoglycan, the polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and aminophospholipids as the major components, and the predominant menaquinone was MK-7. The DNA G+C content was 52.9 mol%. The results of comparative 16S rRNA gene sequence studies placed strain 656.84T within the genus Paenibacillus. Its closest phylogenetic relatives were Paenibacillus barengoltzii and Paenibacillus timonensis. Levels of DNA-DNA relatedness between strain 656.84T and Paenibacillus timonensis CIP 108005T and Paenibacillus barengoltzii CIP 109354T were 17.3 % and 36.8 %, respectively, indicating that strain 656.84T represents a distinct species. On the basis of phenotypic and genotypic results, strain 656.84T is considered to represent a novel species within the genus Paenibacillus, for which the name Paenibacillus faecis sp. nov. is proposed; the type strain is 656.84T ( = DSM 23593T = CIP 101062T).
authors.affiliation: Département de Microbiologie F-75015, Institut Pasteur, Collection de l'Institut Pasteur, Paris, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Clermont
authors.affiliation: Département de Microbiologie F-75015, Institut Pasteur, Collection de l'Institut Pasteur, Paris, France.
authors.forename: Maïté
authors.initials: M
authors.lastname: Gomard
authors.affiliation: Département de Microbiologie F-75015, Institut Pasteur, Collection de l'Institut Pasteur, Paris, France.
authors.forename: Sylviane
authors.initials: S
authors.lastname: Hamon
authors.affiliation: Institut Pasteur, Plate-Forme de Microscopie Ultrastructurale - Imagopole, F-75015, Paris, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bonne
authors.affiliation: Département de Microbiologie F-75015, Institut Pasteur, Collection de l'Institut Pasteur, Paris, France.
authors.forename: José-Carlos
authors.initials: JC
authors.lastname: Fernandez
authors.affiliation: Département de Microbiologie, F-75015 Paris, France; INSERM, équipe Avenir, Institut Pasteur, Unité de Biologie et Génétique de la Paroi Bactérienne, Paris, France.
authors.forename: Richard
authors.initials: R
authors.lastname: Wheeler
authors.affiliation: Institut Pasteur, Unité de Spectrométrie de Masse Structurale et Protéomique - Protéopole, F-75015, Paris, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Malosse
authors.affiliation: Institut Pasteur, Unité de Spectrométrie de Masse Structurale et Protéomique - Protéopole, F-75015, Paris, France.
authors.forename: Julia
authors.initials: J
authors.lastname: Chamot-Rooke
authors.affiliation: Département de Microbiologie, F-75015, Institut Pasteur, Unité de Recherche Biologie Moléculaire du Gène chez les Extrêmophiles, Paris, France.
authors.forename: Simonetta
authors.initials: S
authors.lastname: Gribaldo
authors.affiliation: Département de Microbiologie, F-75015 Paris, France; INSERM, équipe Avenir, Institut Pasteur, Unité de Biologie et Génétique de la Paroi Bactérienne, Paris, France.
authors.forename: Ivo Gomperts
authors.initials: IG
authors.lastname: Boneca
authors.affiliation: Département de Microbiologie F-75015, Institut Pasteur, Collection de l'Institut Pasteur, Paris, France.|Département de Microbiologie F-75015, Institut Pasteur, CRBIP - Centre de Ressources Biologiques de l'Institut Pasteur, Paris, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Bizet
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005243:Feces / Q000382:microbiology*; D005602:France; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7