title: Evaluation of the novel three-dimensional porous poly (L-lactic acid)/nano-hydroxyapatite composite scaffold.
abstract: To determine the optimal ratio of nano-hydroxyapatite (n-HA) to polylactic acid (PLLA) in the novel three-dimensional porous PLLA/n-HA composite scaffolds, low-temperature rapid prototyping technology was employed to fabricate the composite materials with different n-HA contents. Mechanical properties and degradation behaviors of the composites were examined, and the scaffold microstructure and n-HA dispersion were observed by scanning electron microscope (SEM). Mechanical tests demonstrated that the tensile strength of the composite material gradually decreased with an increase in n-HA content. When the n-HA content reached 20 wt%, the bending strength of the composite material peaked at 138.5 MPa. SEM images demonstrated that the optimal content of n-HA was 20 wt% as the largest interconnected pore size that can be seen, with a porosity as high as 80%. In vitro degradation experiments demonstrated that the pH value of the material containing solution gradually decreased in a time-dependent manner, with a simultaneous weakening of the mechanical properties. In vitro study using rat osteoblast cells showed that the composite scaffolds were biocompatible; the 20 wt% n-HA scaffold offered particular improvement to rat osteoblast cell adhesion and proliferation compared to other compositions. It was therefore concluded that 20 wt% n-HA is the optimal nano-hydroxyapatite (n-HA) to polylactic acid (PLLA) ratio, with promise for bone tissue engineering.
authors.affiliation: National Key Orthopedic Department, Shenzhen Second People's Hospital, Guangdong Province, China.|Shenzhen Tissue Engineering Key Laboratory, GuangDong Province, China.
authors.forename: Jianghong
authors.initials: J
authors.lastname: Huang
authors.affiliation: National Key Orthopedic Department, Shenzhen Second People's Hospital, Guangdong Province, China.|Shenzhen Tissue Engineering Key Laboratory, GuangDong Province, China.
authors.forename: Jianyi
authors.initials: J
authors.lastname: Xiong
authors.affiliation: National Key Orthopedic Department, Shenzhen Second People's Hospital, Guangdong Province, China.|Shenzhen Tissue Engineering Key Laboratory, GuangDong Province, China.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Liu
authors.affiliation: National Key Orthopedic Department, Shenzhen Second People's Hospital, Guangdong Province, China.|Shenzhen Tissue Engineering Key Laboratory, GuangDong Province, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhu
authors.affiliation: National Key Orthopedic Department, Shenzhen Second People's Hospital, Guangdong Province, China.|Shenzhen Tissue Engineering Key Laboratory, GuangDong Province, China.
authors.forename: Jielin
authors.initials: J
authors.lastname: Chen
authors.affiliation: National Key Orthopedic Department, Shenzhen Second People's Hospital, Guangdong Province, China.|Shenzhen Tissue Engineering Key Laboratory, GuangDong Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Duan
authors.affiliation: National Key Orthopedic Department, Shenzhen Second People's Hospital, Guangdong Province, China.|Shenzhen Tissue Engineering Key Laboratory, GuangDong Province, China.
authors.forename: Jufeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: National Key Orthopedic Department, Shenzhen Second People's Hospital, Guangdong Province, China.|Shenzhen Tissue Engineering Key Laboratory, GuangDong Province, China.
authors.forename: Daping
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000138:chemical synthesis; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D017886:Durapatite / Q000737:chemistry*; D055119:Elastic Modulus; D004867:Equipment Design; D019544:Equipment Failure Analysis; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D058608:Nanopores / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D013718:Tensile Strength; D054457:Tissue Scaffolds
chemical_list: D018786:Bone Substitutes; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D017886:Durapatite
keywords: Three-dimensional porous; nano-hydroxyapatite; polylactic acid; rapid prototyping