title: Functionalized Graphene Oxide with Hepatocyte Targeting as Anti-Tumor Drug and Gene Intracellular Transporters.
abstract: In order to realize the hepatocyte-specific targeted delivery of anti-tumor drug and gene, lactosylated chitosan oligosaccharide (LCO) functionalized graphene oxides (GO-LCO) containing quaternary ammonium groups (GO-LCO+) were prepared. The formation and composition of GO-LCO+ were confirmed by FTIR, AFM, TGA and zeta-potential. The in vitro cells uptakes of this functionalized GO were investigated and the results showed that GO-LCO+ can deliver fluorescein FAM-labeled DNA sequence (FAM-DNA) into human hepatic carcinoma cells (QGY-7703) with higher efficiency than positively charged chitosan oligosaccharide (CO) functionalized graphene oxides (GO-CO+) without Lactose acid modification. The loading efficiency of doxorubicin chloride (Dox) on GO-LCO+ with 477 µg/mg was obtained at the initial Dox concentration of 0.45 mg/ml and release of Dox on GO-LCO+ showed strong pH dependence. The toxicity of GO-LCO+ before and after loading with Dox toward QGY-7703 cells was further investigated. Our results suggest the functionalized GO to be used as a nanocarrier for hepatocyte targeted co-delivery of anti-tumor drugs and genes with low cytotoxicity, promising for future applications in anticancer drug and gene combined therapy.
authors.forename: Xiufen
authors.initials: X
authors.lastname: Cao
authors.forename: Shuang
authors.initials: S
authors.lastname: Zheng
authors.forename: Sipei
authors.initials: S
authors.lastname: Zhang
authors.forename: Yinsong
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Yang
authors.forename: Hongquan
authors.initials: H
authors.lastname: Duan
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.forename: Yongshen
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004317:Doxorubicin / Q000737:chemistry* / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D065546:Drug Liberation; D019793:Fluorescein / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D009844:Oligosaccharides / Q000737:chemistry; D010087:Oxides / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D009844:Oligosaccharides; D010087:Oxides; D006108:Graphite; D004317:Doxorubicin; D004247:DNA; D048271:Chitosan; D019793:Fluorescein