title: Label-free surface-enhanced Raman spectroscopy for detection of colorectal cancer and precursor lesions using blood plasma.
abstract: Fecal based tests have limited diagnostic values in detecting adenomatous polyps, the precursor lesions to colorectal cancer (CRC). Surface enhanced Raman spectroscopy (SERS) using silver nanoparticles as substrate is a multiplexed analytical technique capable of detecting biomolecules with high sensitivity. This study utilizes SERS to analyze blood plasma for detecting both CRC and adenomatous polyps for the first time. Blood plasma samples are collected from healthy control subjects and patients diagnosed with adenomas and CRC. Using a real-time Raman system, SERS spectra for blood plasma samples are measured in 1 s. The collected SERS spectra are analyzed with partial least squares-discriminant analysis. Classification of normal versus CRC plus adenomatous polyps achieved diagnostic sensitivity of 86.4% and specificity of 80%. The results suggest that blood plasma SERS analysis could be a potential screening test to detect both CRC and adenomas.
authors.affiliation: Imaging Unit - Integrative Oncology Department, BC Cancer Agency Research Centre, 675 West 10th Avenue, Vancouver, BC, V5Z 1L3, Canada ; Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China ; Equal contribution.
authors.forename: Shangyuan
authors.initials: S
authors.lastname: Feng
authors.affiliation: Imaging Unit - Integrative Oncology Department, BC Cancer Agency Research Centre, 675 West 10th Avenue, Vancouver, BC, V5Z 1L3, Canada ; Biomedical Engineering, University of British Columbia, Vancouver BC, V6T 1Z4, Canada ; Equal contribution.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Wang
authors.affiliation: Michael Smith Genome Sciences Centre, 675 West 10th Avenue, Vancouver, BC, V5Z 1L3, Canada ; Division of Gastroenterology, Department of Medicine, University of British Columbia, 2775 Laurel Street, Vancouver, BC, V5Z 1M9, Canada.
authors.forename: Isabella T
authors.initials: IT
authors.lastname: Tai
authors.affiliation: Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China.
authors.forename: Guannan
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.affiliation: Imaging Unit - Integrative Oncology Department, BC Cancer Agency Research Centre, 675 West 10th Avenue, Vancouver, BC, V5Z 1L3, Canada ; Photomedicine Institute - Department of Dermatology and Skin Science, University of British Columbia, Vancouver, BC, V5Z 4E8, Canada.
authors.forename: Haishan
authors.initials: H
authors.lastname: Zeng
keywords: (170.1470) Blood or tissue constituent monitoring; (170.4580) Optical diagnostics for medicine; (170.5660) Raman spectroscopy; (240.6695) Surface-enhanced Raman scattering