title: Regulation of alkane degradation pathway by a TetR family repressor via an autoregulation positive feedback mechanism in a Gram-positive Dietzia bacterium.
abstract: n-Alkanes are ubiquitous in nature and serve as important carbon sources for both Gram-positive and Gram-negative bacteria. Hydroxylation of n-alkanes by alkane monooxygenases is the first and most critical step in n-alkane metabolism. However, regulation of alkane degradation genes in Gram-positive bacteria remains poorly characterized. We therefore explored the transcriptional regulation of an alkB-type alkane hydroxylase-rubredoxin fusion gene, alkW1, from Dietzia sp. DQ12-45-1b. The alkW1 promoter was characterized and so was the putative TetR family regulator, AlkX, located downstream of alkW1 gene. We further identified an unusually long 48 bp inverted repeat upstream of alkW1 and demonstrated the binding of AlkX to this operator. Analytical ultracentrifugation and microcalorimetric results indicated that AlkX formed stable dimers in solution and two dimers bound to one operator in a positive cooperative fashion characterized by a Hill coefficient of 1.64 (± 0.03) [k(D)  = 1.06 (± 0.16) μM, k(D) ' = 0.05 (± 0.01) μM]. However, the DNA-binding affinity was disrupted in the presence of long-chain fatty acids (C10-C24), suggesting that AlkX can sense the concentrations of n-alkane degradation metabolites. A model was therefore proposed where AlkX controls alkW1 expression in a metabolite-dependent manner. Bioinformatic analysis revealed that the alkane hydroxylase gene regulation mechanism may be common among Actinobacteria.
authors.affiliation: Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing, 100871, China.
authors.forename: Jie-Liang
authors.initials: JL
authors.lastname: Liang
authors.affiliation: Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing, 100871, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Nie
authors.affiliation: Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing, 100871, China.
authors.forename: Miaoxiao
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Toxicology and Pharmacology for Natural Scientists, University Medical School, Schleswig-Holstein, Campus Kiel, Kiel, 24105, Germany.
authors.forename: Guangming
authors.initials: G
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Wang
authors.affiliation: Institute of Toxicology and Pharmacology for Natural Scientists, University Medical School, Schleswig-Holstein, Campus Kiel, Kiel, 24105, Germany.
authors.forename: Edmund
authors.initials: E
authors.lastname: Maser
authors.affiliation: Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing, 100871, China.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Wu
mesh_terms: D039903:Actinobacteria / Q000235:genetics* / Q000378:metabolism; D000473:Alkanes / Q000378:metabolism*; D001426:Bacterial Proteins; D001673:Biodegradation, Environmental; D025461:Feedback, Physiological; D015964:Gene Expression Regulation, Bacterial; D005810:Multigene Family; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000473:Alkanes; D001426:Bacterial Proteins; D012097:Repressor Proteins