title: Protective effects of andrographolide derivative AL-1 on high glucose-induced oxidative stress in RIN-m cells.
abstract: AL-1 is a novel andrographolide derivative synthesized by conjugating andrographolide and alpha lipoic acid. AL-1 has been found to increase insulin secretion, decrease blood glucose level and protect β-cell mass and function in alloxan-induced diabetic mouse model. However, the protective mechanism of AL-1 on high glucose-induced pancreatic β-cell injury is still not clear. In the present study, we found that AL-1 reduced reactive oxygen species (ROS) and nitric oxide (NO) generation induced by high glucose in RIN-m cells, and which elevated the activities of superoxide dismutase (SOD) and catalase (CAT). In addition, AL-1 increased the expression of NF-E2-related factor 2 (Nrf2), thioredoxin-1 (Trx-1) and heme oxygenase-1 (HO- 1) proteins in RIN-m cells. These results suggest that AL-1 prevented RIN-m cells from high glucose-induced oxidative damage via upregulation of Nrf2 signaling pathway.
authors.forename: Hui
authors.initials: H
authors.lastname: Yan
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Li
authors.forename: Yali
authors.initials: Y
authors.lastname: Yang
authors.forename: Zaijun
authors.initials: Z
authors.lastname: Zhang
authors.forename: Gaoxiao
authors.initials: G
authors.lastname: Zhang
authors.forename: Yewei
authors.initials: Y
authors.lastname: Sun
authors.forename: Pei
authors.initials: P
authors.lastname: Yu
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of New Drug Research and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine, Jinan University College of Pharmacy, Guangzhou 510632, China. xulipeng2000@163.com.
authors.forename: Lipeng
authors.initials: L
authors.lastname: Xu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004224:Diterpenes / Q000737:chemistry* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000008:administration & dosage* / Q000633:toxicity; D007340:Insulinoma / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010190:Pancreatic Neoplasms / Q000378:metabolism; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology; D051381:Rats
chemical_list: D004224:Diterpenes; D020011:Protective Agents; C030419:andrographolide; D005947:Glucose