title: Novel DiR and SPIO nanoparticles embedded PEG-PLGA nanobubbles as a multimodalimaging contrast agent.
abstract: Fluorescence dye DiR and superparamagnetic iron oxide nanoparticles (SPIONs) embedded in PEG-PLGA nanobubbles (DiR-SPIO-NBs) were produced using double emulsion method on a membrane of Shirasu porous glass (SPG). The nanobubbles encapsulated with DiR and SPIONs had a liquid core (perfluoropentane) and a PEG-PLGA shell. DiR-SPIO-NBs showed biocompatibility based on MTT cytotoxicity and hemolysis studies. The PFP encapsulated in the nanobubbles experienced phase transition under ultrasonic irradation. Nanobubbles dispersed well in saline over 3 months, and the relaxivity was 127.9 mM(-1)s(-1), suggesting that it could be used as a contrast agent in MRI. The MR and fluorescence images in vivo demonstrated that the signal intensity in the spleen and liver was significantly enhanced with the treatment of nanobubbles. In addition, results of ultrasound images suggested that the nanobubbles had persistent contrast ability. In conclusion, nanobubbles could be utilized as an US/MRI/fluorescence contrast agent.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.|College of Pharmacy, Hubei University of Science and Technology, Xianning, China.
authors.forename: Binhua
authors.initials: B
authors.lastname: Luo
authors.affiliation: Wuhan Institute of Physical Education, Wuhan, 430079, China.
authors.forename: Huajie
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Xuhan
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Rao
authors.affiliation: Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D005466:Fluorocarbons / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity; D006461:Hemolysis / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008807:Mice, Inbred BALB C; D045423:Microbubbles; D009369:Neoplasms / Q000175:diagnosis; D061848:Optical Imaging / Q000379:methods*; D044367:Phase Transition; D011091:Polyesters / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D011817:Rabbits; D000069453:Ultrasonic Waves; D014463:Ultrasonography / Q000379:methods*; D010420:Pentanes
chemical_list: D003287:Contrast Media; D005456:Fluorescent Dyes; D005466:Fluorocarbons; D058185:Magnetite Nanoparticles; D011091:Polyesters; D011092:Polyethylene Glycols; C000589473:polyethylene glycol-poly(lactide-co-glycolide); C008806:perfluoropentane; D010420:Pentanes
keywords: Nanobubbles; fluorescence imaging; magnetic resonance imaging; multimodal contrast agent; ultrasound imaging; ultrasound-triggered phase transition