title: The amphiphilic hydrophobin Vmh2 plays a key role in one step synthesis of hybrid protein-gold nanoparticles.
abstract: We report a simple and original method to synthesize gold nanoparticles in which a fungal protein, the hydrophobin Vmh2 from Pleurotus ostreatus and dicarboxylic acid-terminated polyethylene-glycol (PEG) has been used as additional components in a one step process, leading to hybrid protein-metal nanoparticles (NPs). The nanoparticles have been characterized by ultra-violet/visible, infrared and X-ray photoelectron spectroscopies, dynamic light scattering and also by electron microscopy imaging. The results of these analytical techniques highlight nanometric sized, stable, hybrid complexes of about 12 nm, with outer surface rich in functional chemical groups. Interaction with protein and antibodies has also been exploited.
authors.affiliation: Sorbonne Universités, UPMC Paris 06, Laboratoire de Réactivité de Surface, 4 place Jussieu, Paris F-75005, France; Institute for Microelectronics and Microsystems, Unit of Naples-National Research Council, Via P. Castellino 111, 80127 Italy; Department of Chemical Sciences, University of Naples "Federico II", Via Cinthia, Naples 80126 Italy.
authors.forename: Jane
authors.initials: J
authors.lastname: Politi
authors.affiliation: Institute for Microelectronics and Microsystems, Unit of Naples-National Research Council, Via P. Castellino 111, 80127 Italy. Electronic address: luca.destefano@cnr.it.
authors.forename: Luca
authors.initials: L
authors.lastname: De Stefano
authors.affiliation: Department of Chemical Sciences, University of Naples "Federico II", Via Cinthia, Naples 80126 Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Longobardi
authors.affiliation: Department of Chemical Sciences, University of Naples "Federico II", Via Cinthia, Naples 80126 Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Giardina
authors.affiliation: Institute for Microelectronics and Microsystems, Unit of Naples-National Research Council, Via P. Castellino 111, 80127 Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Rea
authors.affiliation: Sorbonne Universités, UPMC Paris 06, Laboratoire de Réactivité de Surface, 4 place Jussieu, Paris F-75005, France; CNRS, UMR 7197, Laboratoire de Réactivité de Surface, Paris F-75005, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Methivier
authors.affiliation: Sorbonne Universités, UPMC Paris 06, Laboratoire de Réactivité de Surface, 4 place Jussieu, Paris F-75005, France; CNRS, UMR 7197, Laboratoire de Réactivité de Surface, Paris F-75005, France.
authors.forename: Claire-Marie
authors.initials: CM
authors.lastname: Pradier
authors.affiliation: Sorbonne Universités, UPMC Paris 06, Laboratoire de Réactivité de Surface, 4 place Jussieu, Paris F-75005, France; CNRS, UMR 7197, Laboratoire de Réactivité de Surface, Paris F-75005, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Casale
authors.affiliation: Sorbonne Universités, UPMC Paris 06, Laboratoire de Réactivité de Surface, 4 place Jussieu, Paris F-75005, France; CNRS, UMR 7197, Laboratoire de Réactivité de Surface, Paris F-75005, France.
authors.forename: Jolanda
authors.initials: J
authors.lastname: Spadavecchia
mesh_terms: D005656:Fungal Proteins / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D011092:Polyethylene Glycols / Q000737:chemistry; D013057:Spectrum Analysis
chemical_list: D005656:Fungal Proteins; D011092:Polyethylene Glycols; D006046:Gold
keywords: Gold nanoparticles; Hydrophobins; Surface functionalization; Synthesis