title: A protein kinase binds the C-terminal domain of the readthrough protein of Turnip yellows virus and regulates virus accumulation.
abstract: Turnip yellows virus (TuYV), a phloem-limited virus, encodes a 74kDa protein known as the readthrough protein (RT) involved in virus movement. We show here that a TuYV mutant deleted of the C-terminal part of the RT protein (TuYV-∆RTCter) was affected in long-distance trafficking in a host-specific manner. By using the C-terminal domain of the RT protein as a bait in a yeast two-hybrid screen of a phloem cDNA library from Arabidopsis thaliana we identified the calcineurin B-like protein-interacting protein kinase-7 (AtCIPK7). Transient expression of a GFP:CIPK7 fusion protein in virus-inoculated Nicotiana benthamiana leaves led to local increase of wild-type TuYV accumulation, but not that of TuYV-∆RTCter. Surprisingly, elevated virus titer in inoculated leaves did not result in higher TuYV accumulation in systemic leaves, which indicates that virus long-distance movement was not affected. Since GFP:CIPK7 was localized in or near plasmodesmata, CIPK7 could negatively regulate TuYV export from infected cells.
authors.affiliation: UMR 1131 SVQV INRA-UDS, 28 rue de Herrlisheim, 68021 Colmar, France.
authors.forename: Caren
authors.initials: C
authors.lastname: Rodriguez-Medina
authors.affiliation: UMR 1131 SVQV INRA-UDS, 28 rue de Herrlisheim, 68021 Colmar, France.
authors.forename: Sylvaine
authors.initials: S
authors.lastname: Boissinot
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Laboratoire propre du CNRS conventionné avec l'Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Chapuis
authors.affiliation: UMR 1131 SVQV INRA-UDS, 28 rue de Herrlisheim, 68021 Colmar, France.
authors.forename: Dalya
authors.initials: D
authors.lastname: Gereige
authors.affiliation: UMR 1131 SVQV INRA-UDS, 28 rue de Herrlisheim, 68021 Colmar, France.
authors.forename: Maryam
authors.initials: M
authors.lastname: Rastegar
authors.affiliation: UMR 1131 SVQV INRA-UDS, 28 rue de Herrlisheim, 68021 Colmar, France.
authors.forename: Monique
authors.initials: M
authors.lastname: Erdinger
authors.affiliation: INRA, Université de Bordeaux, UMR 1332 de Biologie du Fruit et Pathologie, 33882 Villenave d'Ornon, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Revers
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Laboratoire propre du CNRS conventionné avec l'Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Ziegler-Graff
authors.affiliation: UMR 1131 SVQV INRA-UDS, 28 rue de Herrlisheim, 68021 Colmar, France. Electronic address: veronique.brault@colmar.inra.fr.
authors.forename: Véronique
authors.initials: V
authors.lastname: Brault
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D052679:Luteoviridae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D011485:Protein Binding; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D014764:Viral Proteins; D011494:Protein Kinases; C000608160:CIPK7 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases
keywords: CIPK; Kinase; Movement; Phloem; Plant; Polerovirus