title: Limonoids from Munronia henryi and their anti-tobacco mosaic virus activity.
abstract: Five new compounds (1-5), including three limonoids, one diterpenoid, and one phytosterol, along with four known limonoids (6-9), were isolated from the ethanolic extracts of whole plants of Munronia henryi. Their structures were elucidated by extensive spectroscopic analysis. In addition, the anti-tobacco mosaic virus (TMV) activities of all the isolated compounds were evaluated by the conventional half-leaf and leaf-disk methods along with Western blot analysis. Most of the tested compounds showed strong antiviral activities, with IC50 values in the range of 14.8-34.0μg/mL, compared with ningnanmycin as a positive control (IC50=44.6μg/mL), and their preliminary structure-activity relationships were also discussed.
authors.affiliation: Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang 550025, People's Republic of China; The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yan
authors.affiliation: The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
authors.forename: Chun-Mao
authors.initials: CM
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, People's Republic of China.
authors.forename: Ying-Tong
authors.initials: YT
authors.lastname: Di
authors.affiliation: The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.affiliation: The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Fan
authors.affiliation: The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China. Electronic address: haoxj@mail.kib.ac.cn.
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Hao
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry* / Q000302:isolation & purification; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification; D036701:Limonins / Q000737:chemistry* / Q000302:isolation & purification; D029582:Meliaceae / Q000737:chemistry*; D015394:Molecular Structure; D010840:Phytosterols / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry; D013329:Structure-Activity Relationship; D014027:Tobacco Mosaic Virus / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004224:Diterpenes; D036701:Limonins; D010840:Phytosterols; D010936:Plant Extracts
keywords: Anti-tobacco mosaic virus (TMV) activity; Limonoids; Munronia henryi; Structure-activity relationship; Western blot