title: Laccase oxidation and removal of toxicants released during combustion processes.
abstract: This study reports for the first time the ability of laccases adsorbed on cellulose acetate to eliminate toxicants released during combustion processes. Laccases directly oxidized and eliminated more than 40% w/v of 14 mM of 1,4-dihydroxybenzene (hydroquinone); 2-methyl-1,4-benzenediol (methylhydroquinone); 1,4-dihydroxy-2,3,5-trimethylbenzene (trimethylhydroquinone); 3-methylphenol (m-cresol); 4-methylphenol (p-cresol); 2-methylphenol (o-cresol); 1,3-benzenediol (resorcinol); 1,2-dihydroxybenzene (catechol); 3,4-dihydroxytoluene (4-methylcatechol) and 2-naphthylamine. Further, laccase oxidized 2-naphthylamine, hydroquinone, catechol, methylhydroquinone and methylcatechol were also able to in turn mediate the elimination of >90% w/v of toxicants which are per-se non-laccase substrates such as 3-aminobiphenyl; 4-aminobiphenyl; benz[a]anthracene; 3-(1-nitrosopyrrolidin-2-yl) pyridine (NNN); formaldehyde; 4-(methyl-nitrosamino-1-(3-pyridyl)-1-butanone (NNK); 2-butenal (crotonaldehyde); nitric oxide and vinyl cyanide (acrylonitrile). These studies demonstrate the potential of laccase immobilized on solid supports to remove many structurally different toxicants released during combustion processes. This system has great potential application for in situ removal of toxicants in the manufacturing, food processing and food service industries.
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences, Vienna, Konrad Lorenz Strasse 20, 3430 Tulln, Austria.
authors.forename: Endry Nugroho
authors.initials: EN
authors.lastname: Prasetyo
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences, Vienna, Konrad Lorenz Strasse 20, 3430 Tulln, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Semlitsch
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences, Vienna, Konrad Lorenz Strasse 20, 3430 Tulln, Austria; Botswana International University of Science and Technology, Private Bag 16, Palapye, Botswana. Electronic address: g.nyanhongo@boku.ac.at.
authors.forename: Gibson S
authors.initials: GS
authors.lastname: Nyanhongo
authors.affiliation: British American Tobacco, Group Research & Development, Regents Park Road, Millbrook, Southampton SO15 8TL, UK.
authors.forename: Yahia
authors.initials: Y
authors.lastname: Lemmouchi
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences, Vienna, Konrad Lorenz Strasse 20, 3430 Tulln, Austria.
authors.forename: Georg M
authors.initials: GM
authors.lastname: Guebitz
mesh_terms: D000393:Air Pollutants / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D042845:Laccase / Q000378:metabolism*; D009930:Organic Chemicals / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D000393:Air Pollutants; D009930:Organic Chemicals; D042845:Laccase
keywords: Enzyme filters; Laccase; Phenolics; Polyaromatic hydrocarbons; Toxic combustion products