title: Comparison of Whole-Genome Sequencing and Molecular-Epidemiological Techniques for Clostridium difficile Strain Typing.
abstract: We analyzed in parallel 27 pediatric Clostridium difficile isolates by repetitive sequence-based polymerase chain reaction (RepPCR), pulsed-field gel electrophoresis (PFGE), and whole-genome next-generation sequencing. Next-generation sequencing distinguished 3 groups of isolates that were indistinguishable by RepPCR and 1 isolate that clustered in the same PFGE group as other isolates.
authors.affiliation: Department of Pediatric Infectious Diseases, University of Colorado School of Medicine, Aurora Department of Epidemiology, Children's Hospital Colorado, Aurora.
authors.forename: Samuel R
authors.initials: SR
authors.lastname: Dominguez
authors.affiliation: Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Lydia J
authors.initials: LJ
authors.lastname: Anderson
authors.affiliation: Department of Adult Infectious Diseases, University of Colorado School of Medicine, Aurora.
authors.forename: Cassandra V
authors.initials: CV
authors.lastname: Kotter
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Hospital Colorado, Aurora.
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Littlehorn
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Hospital Colorado, Aurora.
authors.forename: Lesley E
authors.initials: LE
authors.lastname: Arms
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Hospital Colorado, Aurora.
authors.forename: Elaine
authors.initials: E
authors.lastname: Dowell
authors.affiliation: Department of Pediatric Infectious Diseases, University of Colorado School of Medicine, Aurora Department of Epidemiology, Children's Hospital Colorado, Aurora.
authors.forename: James K
authors.initials: JK
authors.lastname: Todd
authors.affiliation: Department of Adult Infectious Diseases, University of Colorado School of Medicine, Aurora.
authors.forename: Daniel N
authors.initials: DN
authors.lastname: Frank
mesh_terms: D015373:Bacterial Typing Techniques; D016360:Clostridioides difficile / Q000145:classification*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D000073336:Whole Genome Sequencing
keywords: Clostridium difficile; next generation sequencing; outbreak investigation; pediatrics