title: Preventive effect of Nile tilapia hydrolysate against oxidative damage of HepG2 cells and DNA mediated by H2O2 and AAPH.
abstract: Antioxidant activities of protein hydrolysate prepared from Nile tilapia protein isolate using Alcalase (HA), Alcalase followed by papain (HAPa) and their Sephadex G-25 fractions (FHA and FHAPa) were investigated in both chemical and cellular based models. Amongst all samples, FHAPa showed the highest chemical antioxidant activities, however it had no metal chelation activity. Cellular antioxidant ability of HA, HAPa and their fractions against H2O2 and AAPH induced oxidative damage of HepG2 cell and DNA were tested. When cells were pretreated with all hydrolysates or fractions at different concentrations (0.5-2 mg/mL) in the absence and presence of 50 μM Trolox, cell viability was in the range of 91.10-111.40 %. However, no difference in cell viability was observed among samples having various concentrations (P > 0.05). Cell reactive oxygen species (ROS) generation as mediated by H2O2 and AAPH decreased with treatment of hydrolysates or their fractions, especially in combination with 50 μM Trolox. FHAPa effectively inhibited H2O2 and peroxyl radical induced DNA scission in a dose dependent manner. Therefore, Nile tilapia protein hydrolysates could serve as a functional food ingredient.
authors.affiliation: Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112 Thailand.
authors.forename: Suthasinee
authors.initials: S
authors.lastname: Yarnpakdee
authors.affiliation: Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112 Thailand.
authors.forename: Soottawat
authors.initials: S
authors.lastname: Benjakul
authors.affiliation: Division of Biotechnology and Biomolecules, Matis - Icelandic Food and Biotechnology R & D, Vinlandsleid 12, 113 Reykjavik, Iceland ; Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL 32611 USA.
authors.forename: Hordur G
authors.initials: HG
authors.lastname: Kristinsson
authors.affiliation: Division of Biotechnology and Biomolecules, Matis-Biotechnology Center, Haeyri 1, 550 Saudarkrokur, Iceland.
authors.forename: Hilma Eiðsdóttir
authors.initials: HE
authors.lastname: Bakken
keywords: Antioxidant activity; DNA damage; H2O2; HepG2 cell; Nile tilapia protein hydrolysate