title: An UPLC-MS detection method for the quantification of five antibiotics in human plasma.
abstract: BACKGROUND
  An UPLC-MS detection method for the quantification of amikacin, flucloxacillin, meropenem, penicillin G and vancomycin was developed and validated.
  
  
  RESULTS
  The calibration curves were found to be linear from 0.47 to 50 mg/l for amikacin, 1.28 to 135 mg/l for flucloxacillin, 0.75 to 80 mg/l for meropenem, 0.38 to 80 mg/l for penicillin G and 0.73 to 80 mg/l for vancomycin. Between- and within-run accuracy was ranged between 85 and 115%. Between and within imprecision expressed as CV was within 15%.
  
  
  CONCLUSIONS
  The validated method was successfully applied to a PK study in very preterm and small for gestational age infants treated for nosocomial sepsis and/or meningitis.
authors.affiliation: Department of Clinical Pharmacology & Pharmacy, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors.forename: Abdel
authors.initials: A
authors.lastname: Chahbouni
authors.affiliation: Department of Pediatrics, Division of Neonatology, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors.forename: Frank Am van
authors.initials: FAV
authors.lastname: den Dungen
authors.affiliation: Department of Clinical Pharmacology & Pharmacy, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors.forename: René M
authors.initials: RM
authors.lastname: Vos
authors.affiliation: Department of Clinical Pharmacology & Pharmacy, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors.forename: Jeroen Cg
authors.initials: JC
authors.lastname: den Burger
authors.affiliation: Department of Clinical Pharmacology & Pharmacy, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors.forename: Amo
authors.initials: A
authors.lastname: Sinjewel
authors.affiliation: Department of Clinical Pharmacology & Pharmacy, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors.forename: Abraham J
authors.initials: AJ
authors.lastname: Wilhelm
authors.affiliation: Department of Clinical Pharmacology & Pharmacy, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors.forename: Agnes I
authors.initials: AI
authors.lastname: Veldkamp
authors.affiliation: Department of Clinical Pharmacology & Pharmacy, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors.forename: Eleanor L
authors.initials: EL
authors.lastname: Swart
authors.affiliation: Department of Pediatrics, Division of Neonatology, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors.forename: Mirjam M
authors.initials: MM
authors.lastname: van Weissenbruch