title: Genetic polymorphism of estrogen receptor alpha gene in Egyptian women with type II diabetes mellitus.
abstract: Estrogen might play an important role in type 2 diabetes mellitus pathogenesis. A number of polymorphisms have been reported in the estrogen receptor alpha gene including the XbaI and PvuII restriction enzyme polymorphisms. The aim of this study was to determine if ESRα gene polymorphisms are associated with type 2 diabetes mellitus and correlated with lipid profile. Ninety diabetic Egyptian patients were compared with forty healthy controls. ESRα genotyping of PvuII and XbaI was performed using restriction fragment length polymorphism analysis. Our study showed that there is more significant difference in the frequency of C and G polymorphic allele between patients and control groups in PvuII and XbaI respectively. Also carriers of minor C and G alleles of PvuII and XbaI gene polymorphisms were associated with increased fasting blood glucose and disturbance in lipid profile as there is an increase in total cholesterol, triglycerides and Low density lipoprotein. So findings of present study suggest the possibility that PvuII and XbaI polymorphisms in ERα are related to T2DM and with increased serum lipids among Egyptian population.
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Cairo University, Egypt.
authors.forename: Tarek M K
authors.initials: TM
authors.lastname: Motawi
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Draya University, Egypt.
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: El-Rehany
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Cairo University, Egypt.
authors.forename: Sherine M
authors.initials: SM
authors.lastname: Rizk
authors.affiliation: Biochemistry Department, Faculty of Medicine, Minia University, Egypt.
authors.forename: Maggie M
authors.initials: MM
authors.lastname: Ramzy
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Cairo University, Egypt.
authors.forename: Doaa M
authors.initials: DM
authors.lastname: El-Roby
keywords: ERα, estrogen receptor alpha; Estrogen receptor alpha; FBG, Fasting blood glucose; Gene polymorphism; PvuII; RFLP, restriction fragment length polymorphism; SNP, single nucleotide polymorphism; Serum lipid profile; Type 2 diabetes; XbaI