title: Glossogyne tenuifolia Extract Inhibits TNF-α-Induced Expression of Adhesion Molecules in Human Umbilical Vein Endothelial Cells via Blocking the NF-kB Signaling Pathway.
abstract: Chronic inflammation plays a pivotal role in the development of atherosclerosis, where the pro-inflammatory cytokine-induced expression of endothelial adhesion molecules and the recruitment of monocytes are the crucial events leading to its pathogenesis. Glossogyne tenuifolia ethanol extract (GTE) is shown to have potent anti-inflammatory and antioxidant activities. We evaluated the effects of GTE and its major components, luteolin (lut), luteolin-7-glucoside (lut-7-g), and oleanolic acid (OA) on TNF-α-induced expression of adhesion molecules in human umbilical vein endothelial cells (HUVECs). The results demonstrated that GTE, lut, and lut-7-g attenuated the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in TNF-α-activated HUVECs, and inhibited the adhesion of monocytes to TNF-α-activated HUVECs. The TNF-α-induced mRNA expression of ICAM-1 and VCAM-1 was also suppressed, revealing their inhibitory effects at the transcriptional level. Furthermore, GTE, lut, and lut-7-g blocked the TNF-α-induced degradation of nuclear factor-kB inhibitor (IkB), an indicator of the activation of nuclear factor-kB (NF-kB). In summary, GTE and its bioactive components were effective in preventing the adhesion of monocytes to cytokine-activated endothelium by the inhibition of expression of adhesion molecules, which in turn is mediated through blocking the activation and nuclear translocation of NF-kB. The current results reveal the therapeutic potential of GTE in atherosclerosis.
authors.affiliation: Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan. calvin.hsuan@msa.hinet.net.|Division of Cardiology, Department of Internal Medicine, E-Da Hospital/I-Shou University, Kaohsiung 82445, Taiwan. calvin.hsuan@msa.hinet.net.
authors.forename: Chin-Feng
authors.initials: CF
authors.lastname: Hsuan
authors.affiliation: Department of Nutrition, I-Shou University, Kaohsiung 82445, Taiwan. fen153848@yahoo.com.tw.
authors.forename: Hsia-Fen
authors.initials: HF
authors.lastname: Hsu
authors.affiliation: Division of Cardiology, Department of Internal Medicine, E-Da Hospital/I-Shou University, Kaohsiung 82445, Taiwan. arthurtseng@seed.net.tw.
authors.forename: Wei-Kung
authors.initials: WK
authors.lastname: Tseng
authors.affiliation: Division of Cardiology, Department of Internal Medicine, E-Da Hospital/I-Shou University, Kaohsiung 82445, Taiwan. lip1969@hotmail.com.
authors.forename: Thung-Lip
authors.initials: TL
authors.lastname: Lee
authors.affiliation: Division of Pulmonary Medicine, Department of Internal Medicine, E-Da Hospital/I-Shou University, Kaohsiung 82445, Taiwan. yufeng528@gmail.com.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Wei
authors.affiliation: Division of Cardiology, Department of Internal Medicine, E-Da Hospital/I-Shou University, Kaohsiung 82445, Taiwan. ed103914@edah.org.tw.
authors.forename: Kwan-Lih
authors.initials: KL
authors.lastname: Hsu
authors.affiliation: Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital, Taipei 10002, Taiwan. chauchungwu@ntu.edu.tw.
authors.forename: Chau-Chung
authors.initials: CC
authors.lastname: Wu
authors.affiliation: Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan. jyhoung@isu.edu.tw.|Department of Nutrition, I-Shou University, Kaohsiung 82445, Taiwan. jyhoung@isu.edu.tw.
authors.forename: Jer-Yiing
authors.initials: JY
authors.lastname: Houng
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D018925:Chemokines / Q000235:genetics*; D019743:Chemokines, CXC; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D047311:Luteolin / Q000494:pharmacology; D016328:NF-kappa B; D009828:Oleanolic Acid / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: C508524:CXCL17 protein, human; D018925:Chemokines; D019743:Chemokines, CXC; D005960:Glucosides; D016328:NF-kappa B; D010936:Plant Extracts; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D009828:Oleanolic Acid; C066408:luteolin-7-glucoside; D047311:Luteolin
keywords: Glossogyne tenuifolia; adhesion molecule; luteolin; luteolin-7-glucoside; nuclear factor-kB