title: [Determination of perfluoroalkyl acids in lamb liver by high performance liquid chromatography- tandem mass spectrometry combined with dispersive solid phase extraction].
abstract: A method was developed for the determination of 19 perfluoroalkyl acids (PFAs) in lamb liver by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/ MS) combined with dispersive solid phase extraction. The sample was extracted with acidified acetonitrile, and then cleaned-up by a mixture of N-propylethylenediamine (PSA), C18 and graphitized carbon black (GCB) sorbents. The 19 PFAs were analyzed by HPLC-MS/MS with a C18 chromatographic column, adopting the multiple reaction monitoring (MRM) mode with negative electrospray ionization. The effects of the dosages of hydrochloric acid and the sorbents on the recoveries of the 19 PFAs were studied. For accurate quantitative analysis, the isotope internal standard method was used. The calibration curves were linear with the correlation coefficients over 0.998 in the range of 0.05-20 µg/kg for the 19 PFAs. The limits of detection were 0.004-0.111 µg/kg. The limits of quantification were 0.012-0.370 µg/kg. The mean recoveries of the 19 PFAs at spiked levels of 0.5, 1.0, 2.0 µg/kg were in the range from 80% to 128% with the relative standard deviations of 0.31%-11.1%. The developed method is rapid, simple, accurate. It is suitable for the determination of the 19 PFAs in large quantities of lamb liver samples.
authors.forename: Pingping
authors.initials: P
authors.lastname: Zhu
authors.forename: Zhenfeng
authors.initials: Z
authors.lastname: Yue
authors.forename: Zongkun
authors.initials: Z
authors.lastname: Zheng
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wenyin
authors.initials: W
authors.lastname: Li
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Zhao
authors.lastname: Xiao Chengui
authors.forename: Runye
authors.initials: R
authors.lastname: Bai
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
mesh_terms: D000818:Animals; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005466:Fluorocarbons / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D013058:Mass Spectrometry; D008460:Meat / Q000032:analysis; D012756:Sheep; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D002264:Carboxylic Acids; D005466:Fluorocarbons