title: Induction of spinal long-term synaptic potentiation is sensitive to inhibition of neuronal NOS in L5 spinal nerve-transected rats.
abstract: The role of neuronal nitric oxide synthase (nNOS) in the central mechanism of neuropathic pain and long-term potentiation (LTP) of peripheral afferents remains obscure. The current study investigated the effect of intrathecal application of 7-nitroindazole (7-NI), a selective nNOS inhibitor (8.15 µg/5µl), on mechanical allodynia on day 14 after L5 spinal nerve transection. Furthermore, using in vivo single unit extracellular recording, we examined the effect of 7-NI on the induction of LTP of Aδ- and C-fiber-evoked responses. We have demonstrated that 7-NI attenuates nerve-injury-evoked mechanical allodynia. Additionally, our electrophysiological study has shown that the spinal administration of 7-NI significantly inhibits the induction of the LTP of Aδ- and C-fiber-evoked responses on day 14 after neuropathy. These data suggest that activation of nNOS may be crucial for the induction of the spinal LTP of Aδ- and C-fiber-evoked responses following peripheral nerve damage.
authors.affiliation: Department of Neurophysiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Bahari
authors.affiliation: Department of Physiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran ; Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Homa
authors.initials: H
authors.lastname: Manaheji
authors.affiliation: Department of Neurophysiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran ; Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Hosseinmardi
authors.affiliation: Neuroscience Research Center, Baqiyatallah (a.s.) University of Medical Sciences, Tehran, Iran.
authors.forename: Gholam Hossein
authors.initials: GH
authors.lastname: Meftahi
authors.affiliation: Department of Physiology, Bushehr University of Medical Sciences, Bushehr, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Sadeghi
authors.affiliation: Department of Neurophysiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Samira
authors.initials: S
authors.lastname: Danialy
authors.affiliation: Science Beam CO., Tehran, Iran.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Noorbakhsh
keywords: LTP; dorsal horn; nNOS; neuropathic pain