title: Effect of spent turmeric on kidney glycoconjugates in streptozotocin-induced diabetic rats.
abstract: BACKGROUND
  Curcumin known to have number of medicinal use and masked the fiber containing ukonan like active polysaccharide in turmeric and its pharmacological effect will be addressed on diabetic nephropathy particularly the glycoconjugates of extracellular components viz., glycoproteins and glycosaminoglycans - heparan sulfate (HS).
  
  
  METHODS
  Male Wistar rats were maintained on AIN-76 diet containing 10% spent turmeric and were grouped into control and STZ induced diabetes SFC/TFC and SFD/TFD, respectively. Diabetic status was monitored using blood and urine, and at the end, harvested kidneys were used to study the amelioration of glycoprotiens (collagen) and HS by enzymatic digestion, spectrophotometric, hydroxyproline and agarose electrophoretic methods.
  
  
  RESULTS
  In the present study spent turmeric (10%) fed diabetic rats showed improved glomerular filtration rate (50%), kidney enlargement (60%) and other glycoconjugate metabolism in kidney. Increased collagen content in diabetic group was observed by hydroxyproline estimation (24%) and periodic acid-Schiff's (PAS) staining. Furthermore, elevated activities of enzymes involved in the synthesis and degradation of glycosaminoglycans (GAGs) were significantly lowered in spent turmeric fed diabetic group. Improvement in total GAGs (43%) and sulfate content (18%) followed by fractionation of GAGs using specific enzymes led to HS (28%) in the spent turmeric fed diabetic group, when compared to starch fed diabetic group and was further confirmed by electrophoresis of GAG.
  
  
  CONCLUSIONS
  These results clearly indicate beneficial role of spent turmeric in controlling glycoconjugates such as glycoproteins and heparan sulfate related kidney complications during diabetes.
authors.affiliation: Department of Lipid Science and Traditional Foods, Central Food Technological Research Institute, Mysore 570 020, India.
authors.forename: Gurusiddaiah Suresh
authors.initials: GS
authors.lastname: Kumar
authors.affiliation: Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore 570 020, India.
authors.forename: Paramahans Veerayya
authors.initials: PV
authors.lastname: Salimath
keywords: Diabetic nephropathy; Glycoproteins; Heparan sulfate