title: Magnesium oxide nanoparticles coated with glucose can silence important genes of Leishmania major at sub-toxic concentrations.
abstract: The aim of this study was to investigate the effect of magnesium oxide nanoparticles (MgO NPs) and MgO NPs coated with glucose (MONPCG) on Leishmania (L) major. First, the promastigotes of L. major were separately incubated with serial concentrations of MgO NPs and MONPCG for 24, 48, and 72 h at 37 °C. Then, the cell viability of promastigotes was evaluated by MTT assay. On the other hand, the relative expression of Cpb and GP63 genes was detected by quantitative-real time PCR. Based on results, the increase of concentration, both MgO NPs and MONPCG, and incubation time led to decrease of cell viability. Moreover, the expression of Cpb and GP63 genes was decreased with increase of concentration of MgO NPs and MONPCG. Also, the increase of incubation time led to decrease of their expression in MgO NPs treated promastogotes. But, in case of MONPCG treated promastogotes, the increase of incubation time did not change the expression of Cpb and GP63. Interestingly, MONPCG could silence Cpb and GP63 genes better than MgO NPs. Note, the capability was also seen at sub-toxic concentrations of MONPCG.
authors.affiliation: Department of Medical Parasitology & Mycology, The School of Medicine, Yazd Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Ali Fatahi
authors.initials: AF
authors.lastname: Bafghi
authors.affiliation: Department of Medical Nanotechnology, Pajoohesh Lab, Yazd, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Daghighi
authors.affiliation: Department of Medical Nanotechnology, Pajoohesh Lab, Yazd, Iran.
authors.forename: Karim
authors.initials: K
authors.lastname: Daliri
authors.affiliation: Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd 8916733754, Iran. Electronic address: alijebal2011@gmail.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Jebali
mesh_terms: D020868:Gene Silencing; D017125:Genes, Protozoan; D005947:Glucose / Q000737:chemistry*; D018320:Leishmania major / Q000235:genetics*; D008277:Magnesium Oxide / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning
chemical_list: D008277:Magnesium Oxide; D005947:Glucose
keywords: Glucose; Leishmania major; Magnesium oxide nanoparticles; Sub-toxic