title: Genome Sequences of Three Phytopathogenic Species of the Magnaporthaceae Family of Fungi.
abstract: Magnaporthaceae is a family of ascomycetes that includes three fungi of great economic importance: Magnaporthe oryzae, Gaeumannomyces graminis var. tritici, and Magnaporthe poae. These three fungi cause widespread disease and loss in cereal and grass crops, including rice blast disease (M. oryzae), take-all disease in wheat and other grasses (G. graminis), and summer patch disease in turf grasses (M. poae). Here, we present the finished genome sequence for M. oryzae and draft sequences for M. poae and G. graminis var. tritici. We used multiple technologies to sequence and annotate the genomes of M. oryzae, M. poae, and G. graminis var. tritici. The M. oryzae genome is now finished to seven chromosomes whereas M. poae and G. graminis var. tritici are sequenced to 40.0× and 25.0× coverage respectively. Gene models were developed by the use of multiple computational techniques and further supported by RNAseq data. In addition, we performed preliminary analysis of genome architecture and repetitive element DNA.
authors.affiliation: Center for Integrated Fungal Research, North Carolina State University, Raleigh, North Carolina 27606 Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27606.
authors.forename: Laura H
authors.initials: LH
authors.lastname: Okagaki
authors.affiliation: Center for Integrated Fungal Research, North Carolina State University, Raleigh, North Carolina 27606 Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27606.
authors.forename: Cristiano C
authors.initials: CC
authors.lastname: Nunes
authors.affiliation: Center for Integrated Fungal Research, North Carolina State University, Raleigh, North Carolina 27606 Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27606.
authors.forename: Joshua
authors.initials: J
authors.lastname: Sailsbery
authors.affiliation: Center for Integrated Fungal Research, North Carolina State University, Raleigh, North Carolina 27606.
authors.forename: Brent
authors.initials: B
authors.lastname: Clay
authors.affiliation: Center for Integrated Fungal Research, North Carolina State University, Raleigh, North Carolina 27606.
authors.forename: Doug
authors.initials: D
authors.lastname: Brown
authors.affiliation: Center for Integrated Fungal Research, North Carolina State University, Raleigh, North Carolina 27606.
authors.forename: Titus
authors.initials: T
authors.lastname: John
authors.affiliation: Center for Integrated Fungal Research, North Carolina State University, Raleigh, North Carolina 27606 Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27606.
authors.forename: Yeonyee
authors.initials: Y
authors.lastname: Oh
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01003.
authors.forename: Nelson
authors.initials: N
authors.lastname: Young
authors.affiliation: The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142.
authors.forename: Michael
authors.initials: M
authors.lastname: Fitzgerald
authors.affiliation: The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Haas
authors.affiliation: The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142.
authors.forename: Qiandong
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142.
authors.forename: Sarah
authors.initials: S
authors.lastname: Young
authors.affiliation: The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142.
authors.forename: Xian
authors.initials: X
authors.lastname: Adiconis
authors.affiliation: The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142.
authors.forename: Lin
authors.initials: L
authors.lastname: Fan
authors.affiliation: The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142.
authors.forename: Joshua Z
authors.initials: JZ
authors.lastname: Levin
authors.affiliation: Department of Plant Pathology, Ohio State University, Columbus, Ohio 43210.
authors.forename: Thomas K
authors.initials: TK
authors.lastname: Mitchell
authors.affiliation: USDA-ARS, Root Disease and Biological Control, Pullman, Washington 99164.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Okubara
authors.affiliation: Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546.
authors.forename: Mark L
authors.initials: ML
authors.lastname: Farman
authors.affiliation: Department of Biology, University of Toronto, Mississauga, Toronto, Ontario, L5L 1C6, Canada.
authors.forename: Linda M
authors.initials: LM
authors.lastname: Kohn
authors.affiliation: The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142.
authors.forename: Bruce
authors.initials: B
authors.lastname: Birren
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01003 radean2@ncsu.edu lijun@biochem.umass.edu.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Ma
authors.affiliation: Center for Integrated Fungal Research, North Carolina State University, Raleigh, North Carolina 27606 Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27606 radean2@ncsu.edu lijun@biochem.umass.edu.
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Dean
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000235:genetics*; D019295:Computational Biology / Q000379:methods; D016681:Genome, Fungal; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D010935:Plant Diseases / Q000382:microbiology; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000382:microbiology
keywords: Gaeumannomyces; Magnaporthe; repetitive DNA; sequence; synteny