title: Genome sequencing reveals strain dynamics of methicillin-resistant Staphylococcus aureus in the same household in the context of clinical disease in a person and a dog.
abstract: The strain dynamics of methicillin-resistant Staphylococcus aureus (MRSA) isolates from people and the household dog were investigated. The isolates were identified in the context of a randomized controlled trial that tested household-wide decolonization of people. Genotypic comparison of MRSA isolates obtained from two household members, the dog, and home surfaces over a three-month period failed to implicate the pet or the home environment in recurrent colonization of the household members. However, it did implicate the pet's bed in exposure of the dog prior to the dog's infection. Whole genome sequencing was performed to differentiate the isolates. This report also describes introduction of diverse strains of MRSA into the household within six weeks of cessation of harmonized decolonization treatment of people and treatment for infection in the dog. These findings suggest that community sources outside the home may be important for recurrent MRSA colonization or infection.
authors.affiliation: Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA. Electronic address: mdavis65@jhu.edu.
authors.forename: Meghan F
authors.initials: MF
authors.lastname: Davis
authors.affiliation: Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, 3900 Delancey St., Philadelphia, Pennsylvania 19104, USA.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Misic
authors.affiliation: Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, 3900 Delancey St., Philadelphia, Pennsylvania 19104, USA.
authors.forename: Daniel O
authors.initials: DO
authors.lastname: Morris
authors.affiliation: Brandywine Valley Veterinary Hospital, 2580 Strasburg Rd., Coatesville, PA 19320, USA.
authors.forename: John T
authors.initials: JT
authors.lastname: Moss
authors.affiliation: Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, 3400 Civic Center Blvd, Bldging 421, Philadelphia, 19104, USA.
authors.forename: Pam
authors.initials: P
authors.lastname: Tolomeo
authors.affiliation: Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, 3900 Delancey St., Philadelphia, Pennsylvania 19104, USA.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Beiting
authors.affiliation: Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, 3400 Civic Center Blvd, Bldging 421, Philadelphia, 19104, USA.
authors.forename: Irving
authors.initials: I
authors.lastname: Nachamkin
authors.affiliation: Center for Clinical Epidemiology and Biostatistics, Perelman School of Medicine, University of Pennsylvania, 3400 Civic Center Blvd, Bldging 421, Philadelphia, 19104, USA.
authors.forename: Ebbing
authors.initials: E
authors.lastname: Lautenbach
authors.affiliation: Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, 3900 Delancey St., Philadelphia, Pennsylvania 19104, USA.
authors.forename: Shelley C
authors.initials: SC
authors.lastname: Rankin
mesh_terms: D000328:Adult; D000818:Animals; D015373:Bacterial Typing Techniques; D001512:Bedding and Linens; D004285:Dogs / Q000382:microbiology*; D005260:Female; D016680:Genome, Bacterial; D005838:Genotype; D006801:Humans; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016032:Randomized Controlled Trials as Topic; D013203:Staphylococcal Infections / Q000662:veterinary*
keywords: Antimicrobial resistance; Canine; MRSA; SSI; SSTI