title: [Effect of Genotype and Medium Culture Content on Microspore-Derived Embryo Formation in Chinese Cabbage (Brassica rapa ssp. chinensis) Cv. Lastochka].
abstract: The influence of different factors on microspore embryogenesis in Chinese cabbage (B. rapa ssp. chinensis) was studied. A genotype dependence for embryo formation was observed. The majority of embryos and plants were obtained from microspores isolated from flower buds (2-2.9 mm in length) and cultured in the NLN liquid medium with 13% sucrose (w/v) supplemented with 24-epibrassinolide and 1% activated charcoal. Embryos cultured on the 1/2 Murashige-Skoog culture medium with 2% sucrose (w/v), 0.1 mg/L benzylaminopurine, and 3g/L Phytagel stimulated the formation of secondary embryos that resulted in development of large number of doubled haploid plants.
authors.forename: D V
authors.initials: DV
authors.lastname: Shumilina
authors.forename: N A
authors.initials: NA
authors.lastname: Shmykova
authors.forename: L L
authors.initials: LL
authors.lastname: Bondareva
authors.forename: T P
authors.initials: TP
authors.lastname: Suprunova
mesh_terms: D029741:Brassica rapa / Q000166:cytology / Q000235:genetics*; D018929:Cell Culture Techniques; D003470:Culture Media; D005838:Genotype; D018525:Germination / Q000235:genetics* / Q000502:physiology; D012639:Seeds / Q000235:genetics / Q000254:growth & development
chemical_list: D003470:Culture Media