title: [Determination of phenols and triterpenoid saponins in stems of Sargentodoxa cuneata].
abstract: The methods to determine the total phenols, total saponins, and marker constituents salidroside, chlorogenic acid and 3, 4-dihydroxy-phenylethyl-β-D-glucopyranoside in the samples of Sargentodoxae Caulis were established to provide the evidence for the improvement and revision of the quality standard of the crude material recorded in the Chinese Pharmacopoeia (2015 Edition). The content of total phenols was determined by ultraviolet spectrophotometry, using gallic acid as a reference substance. The content of total saponins was determined by ultraviolet spectrophotometry, using 3-O-[β-D-xylopyranosyl-(1-2)-O-β-D-glucuronopyranosyl]-28-O-[β-D-glucopyranosyl] asiatic acid as a reference substance. The contents of salidroside, chlorogenic acid and 3,4-dihydroxy-phenylethyl-β-D-glucopyranoside were detected by HPLC. The linear ranges were 1.01-7.04 mg x L(-1) for total phenols, 37.7-201 μg for total saponins, 0.025 8-1.55 μg for salidroside, 0.076 2-5.44 μg for chlorogenic acid, and 0.064 9-3.47 μg for 3,4-dihydroxy-phenylethyl-βP-D-glucopyranoside, respectively. Their average recoveries were 99.12%, 99.11% 105.5%, 99.08%, and 101.6%, respectively. The contents of total phenols and total saponins were 3. 04% -11. 9% and 0. 87% -3. 63%. The contents of salidroside, chlorogenic acid and 3,4-dihydroxy-phenylethyl-β-D-glucopyranoside fluctuated from 0.018% to 0. 572%, from 0.041% to 1.75% and from 0.035% to 1.32%. The established methods were reproducible, and they could be used for the quality control of Sargentodoxae Caulis. The present investigation suggested that total phenols, salidroside, and chlorogenic acid should be recorded in the quality standard of Sargentodoxae Caulis and their contents should not be less than 6.8% for total phenols, 0.040% for salidroside, and 0.21% for chlorogenic acid.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Feng-chun
authors.initials: FC
authors.lastname: Zhao
authors.forename: Xian-da
authors.initials: XD
authors.lastname: Yuan
authors.forename: Xiao-qian
authors.initials: XQ
authors.lastname: Liu
authors.forename: Liang-mian
authors.initials: LM
authors.lastname: Chen
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Yan
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Zhu
authors.forename: Hui-min
authors.initials: HM
authors.lastname: Gao
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: Wang
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D019684:Magnoliopsida / Q000737:chemistry*; D019800:Phenol / Q000032:analysis*; D018547:Plant Stems / Q000737:chemistry; D012503:Saponins / Q000032:analysis*; D014315:Triterpenes / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes; D019800:Phenol