title: Vitamin A deficiency: An oxidative stress marker in sodium fluoride (NaF) induced oxidative damage in developing rat brain.
abstract: Fluoride induced oxidative stress through depletion in levels of various anti-oxidants such as glutathione, superoxide dismutase (SOD), fat soluble vitamins (D and E) with increased levels of lipid peroxidation (LPO) and fluoride aggravate the damage in rodents as well as in humans. Vitamins A, a fat soluble vitamin possess antioxidant property which plays a significant role in scavenging the free radicals species similar to vitamin D and E. Vitamin A is involved in neural tissue development and plasticity. The growing evidence about vitamin A being antioxidant in different biological reactions formed the basis to determine the effect of fluoride on its levels. The present study was conducted in Wistar rat pups. The pregnant wistar rats were dosed with 20 ppm sodium fluoride (NaF) from day one of pregnancy till the pups were aged day 30. The serum was collected from developing rat pups on regular intervals (14th, 21st, 30th day) and vitamin A levels were analyzed by High performance liquid chromatography (HPLC). Body weights, Behavioural studies and spectrophotometric estimation of SOD, LPO in brain lysates were also performed. The results showed significant decrease (p<0.001) in vitamin A in fluoride induced samples in comparison to the control samples suggesting that decreased levels of vitamin A can be used as another marker in fluoride induced toxicity studies.
authors.affiliation: Neuroscience Lab, Department of Zoology, Osmania University, Hyderabad, 500020 Telangana, India. Electronic address: rajkiran.banala@osmania.ac.in.
authors.forename: Rajkiran Reddy
authors.initials: RR
authors.lastname: Banala
authors.affiliation: Neuroscience Lab, Department of Zoology, Osmania University, Hyderabad, 500020 Telangana, India. Electronic address: pratapkreddyou@gmail.com.
authors.forename: Pratap Reddy
authors.initials: PR
authors.lastname: Karnati
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002327:Cariostatic Agents / Q000378:metabolism / Q000633:toxicity*; D004195:Disease Models, Animal; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D009043:Motor Activity / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D045442:Rotarod Performance Test; D012969:Sodium Fluoride / Q000097:blood / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D014801:Vitamin A / Q000378:metabolism; D014802:Vitamin A Deficiency / Q000139:chemically induced* / Q000175:diagnosis / Q000503:physiopathology
chemical_list: D002327:Cariostatic Agents; D014801:Vitamin A; D012969:Sodium Fluoride; D013482:Superoxide Dismutase
keywords: Antioxidants; HPLC; Oxidative damage; Sodium fluoride; Vitamin A