title: Imaging Macrophage and Hematopoietic Progenitor Proliferation in Atherosclerosis.
abstract: BACKGROUND
  Local plaque macrophage proliferation and monocyte production in hematopoietic organs promote progression of atherosclerosis. Therefore, noninvasive imaging of proliferation could serve as a biomarker and monitor therapeutic intervention.
  
  
  OBJECTIVE
  To explore (18)F-FLT positron emission tomography-computed tomography imaging of cell proliferation in atherosclerosis.
  
  
  RESULTS
  (18)F-FLT positron emission tomography-computed tomography was performed in mice, rabbits, and humans with atherosclerosis. In apolipoprotein E knock out mice, increased (18)F-FLT signal was observed in atherosclerotic lesions, spleen, and bone marrow (standardized uptake values wild-type versus apolipoprotein E knock out mice, 0.05 ± 0.01 versus 0.17 ± 0.01, P<0.05 in aorta; 0.13 ± 0.01 versus 0.28 ± 0.02, P<0.05 in bone marrow; 0.06 ± 0.01 versus 0.22 ± 0.01, P<0.05 in spleen), corroborated by ex vivo scintillation counting and autoradiography. Flow cytometry confirmed significantly higher proliferation of macrophages in aortic lesions and hematopoietic stem and progenitor cells in the spleen and bone marrow in these mice. In addition, (18)F-FLT plaque signal correlated with the duration of high cholesterol diet (r(2)=0.33, P<0.05). Aortic (18)F-FLT uptake was reduced when cell proliferation was suppressed with fluorouracil in apolipoprotein E knock out mice (P<0.05). In rabbits, inflamed atherosclerotic vasculature with the highest (18)F-fluorodeoxyglucose uptake enriched (18)F-FLT. In patients with atherosclerosis, (18)F-FLT signal significantly increased in the inflamed carotid artery and in the aorta.
  
  
  CONCLUSIONS
  (18)F-FLT positron emission tomography imaging may serve as an imaging biomarker for cell proliferation in plaque and hematopoietic activity in individuals with atherosclerosis.
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Ye
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Claudia
authors.initials: C
authors.lastname: Calcagno
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Tina
authors.initials: T
authors.lastname: Binderup
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Gabriel
authors.initials: G
authors.lastname: Courties
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Edmund J
authors.initials: EJ
authors.lastname: Keliher
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Gregory R
authors.initials: GR
authors.lastname: Wojtkiewicz
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Iwamoto
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Carlos
authors.initials: C
authors.lastname: Pérez-Medina
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Mani
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Seigo
authors.initials: S
authors.lastname: Ishino
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Camilla Bardram
authors.initials: CB
authors.lastname: Johnbeck
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Ulrich
authors.initials: U
authors.lastname: Knigge
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Zahi A
authors.initials: ZA
authors.lastname: Fayad
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Peter
authors.initials: P
authors.lastname: Libby
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Ahmed
authors.initials: A
authors.lastname: Tawakol
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Shipra
authors.initials: S
authors.lastname: Dubey
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Belanger
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Marcelo F
authors.initials: MF
authors.lastname: Di Carli
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Filip K
authors.initials: FK
authors.lastname: Swirski
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Andreas
authors.initials: A
authors.lastname: Kjaer
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.).
authors.forename: Willem J M
authors.initials: WJ
authors.lastname: Mulder
authors.affiliation: From the Center for Systems Biology, Department of Radiology (Y.-X.Y., G.C., E.J.K., G.R.W., Y.I., R.W., F.K.S., M.N.) and Division of Cardiology (A.T.), Massachusetts General Hospital and Harvard Medical School, Boston; Translational and Molecular Imaging Institute, Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY (C.C., J.T., C.P.-M., V.M., S.I., Z.A.F., W.J.M.M.); Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging (T.B., C.B.J., A.K.) and Departments of Clinical Endocrinology PE and Surgery C (U.K.), Rigshospitalet, National University Hospital & University of Copenhagen, Copenhagen, Denmark; Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA (P.L., M.F.D.C.); Department of Systems Biology, Harvard Medical School, Boston, MA (R.W.); Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (S.D., A.P.B., M.F.D.C.); and Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands (W.J.M.M.). mnahrendorf@mgh.harvard.edu.
authors.forename: Matthias
authors.initials: M
authors.lastname: Nahrendorf
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000000981:diagnostic imaging; D001018:Aortic Diseases / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000235:genetics / Q000378:metabolism; D001057:Apolipoproteins E / Q000172:deficiency / Q000235:genetics; D050197:Atherosclerosis / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000235:genetics / Q000378:metabolism; D001853:Bone Marrow / Q000000981:diagnostic imaging; D002340:Carotid Artery Diseases / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D002791:Cholesterol, Dietary; D015224:Dideoxynucleosides; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D005260:Female; D019788:Fluorodeoxyglucose F18; D006412:Hematopoietic Stem Cells / Q000000981:diagnostic imaging; D006801:Humans; D008264:Macrophages / Q000000981:diagnostic imaging; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D064847:Multimodal Imaging; D058226:Plaque, Atherosclerotic; D049268:Positron-Emission Tomography; D011237:Predictive Value of Tests; D011817:Rabbits; D019275:Radiopharmaceuticals; D012189:Retrospective Studies; D013154:Spleen / Q000000981:diagnostic imaging; D013997:Time Factors; D014057:Tomography, X-Ray Computed
chemical_list: D001057:Apolipoproteins E; D002791:Cholesterol, Dietary; D015224:Dideoxynucleosides; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; C002854:alovudine
keywords: 18F-fluorothymidine; atherosclerosis; imaging; inflammation; macrophage; positron emission tomography; proliferation