title: [Effect of a new low-cholesterol meat and vegetal product on correction of simulated lipid metabolism disorders in rats].
abstract: The paper presents the biomedical evaluation of meat and cereal spread from low-cholesterol raw material with vegetable ingredients, recommended as a functional food. The experimental model with myocardial infarction like changes in hearts of the animals, accompanied by vascular changes similar to atherosclerotic changes in humans, as well as the modeling of the metabolic imbalance of lipids have been carried out by intramuscular injection of epinephrine and unbalanced feeding the animals with food rich in cholesterol, with a high content of carbohydrates and fats. Wistar rats were divided into 4 groups of 12 animals each. The rats in groups 1-3 were induced the cardio distress with intramuscular injection of epinephrine; group IV consisted of intact (healthy) animals. Dramatic changes in biochemical blood status that indicated heart disease have been observed within 2 days after the injection of epinephrine (0.2 ml per 1 kg of animal body weight) to the tested animals. During the experiment a sharp increase in activity of indicator enzymes of alanine aminotransferase (ALT) and aspartate aminotransferases (AST), with a predominance of AST over ALT, along with an increase in LDH activity have been observed. The 1.4-1.6 fold increase in blood serum creatinine has also been found. Later the animals in groups 1, 2, 3 with simulated cardio pathology were fed a ration with intervention of food rich in cholesterol, with a high content of carbohydrates and fats (50% of the diet) for a month for induction of lipid metabolism disorders. An increase in the concentration of cholesterol and triglycerides by 3 fold or more has been observed. In addition, an accumulation of sulfhydryl groups has been noted, as evidenced by increased rates of thymol. For further normalization of lipid metabolism, the animals in tested group I were fed the diet with intervention of spread, developed in accordance with GOST 12318-91 "Canned meat "Meat spread"; the rats of group 2 were fed with spread with vegetable supplements (pumpkin by-products and alginates), and group 3 received a standard diet within the next month. The results of biochemical blood serum studies have shown that the level of cholesterol, triglycerides, creatinine and sulphydryl groups in the experimental groups 1 and 2 decreased, indicating a positive influence on lipid metabolism and prevention of peroxides formation in the organism. These changes were more pronounced when meat product with plant additives and alginate was used in the diet. Only under the application of the meat-vegetable pate cholesterol level ceased to differ from that of the control group of animals. Based on the significant reduction of blood malondialdehyde level, antioxidant properties of vegetable supplements have been proven. The experimental data showed that the spread, developed on the basis of low-cholesterol raw meat with vegetable ingredients (pumpkin by-products and alginates) can be used to correct metabolic disorders of lipids, reducing the risk of cardio pathology in the body.
authors.forename: I F
authors.initials: IF
authors.lastname: Gorlov
authors.forename: M I
authors.initials: MI
authors.lastname: Slozhenkina
authors.forename: E V
authors.initials: EV
authors.lastname: Karpenko
authors.forename: T M
authors.initials: TM
authors.lastname: Giro
authors.forename: S V
authors.initials: SV
authors.lastname: Andreeva
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000032:analysis; D004195:Disease Models, Animal; D055951:Functional Food / Q000032:analysis; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood; D008461:Meat Products / Q000032:analysis; D009203:Myocardial Infarction / Q000178:diet therapy* / Q000378:metabolism; D014674:Plant Proteins, Dietary / Q000032:analysis; D017208:Rats, Wistar
chemical_list: D014674:Plant Proteins, Dietary; D008315:Malondialdehyde; D002784:Cholesterol