title: Chlorella pyrenoidosa cultivation in outdoors using the diluted anaerobically digested activated sludge.
abstract: A freshwater green algae Chlorella pyrenoidosa (C. pyrenoidosa) was cultured in outdoors using the diluted anaerobically digested activated sludge (ADAS). The outdoors batch culture in every season showed that C. pyrenoidosa can grow normally under natural conditions in the diluted ADAS (STE/ADAS=1.5/1, 3/1 and 5/1, v/v). Seasonal changes of environmental conditions significantly affected biomass growth and nutrient removal. Optimal biomass growth and nutrient removal was achieved at STE/ADAS=1.5/1 during summer culture, harvesting a maximum biomass concentration of 1.97 ± 0.21 g/L, average biomass productivity of 291.52 ± 33.74 g/m(3)/day (maximum value of 573.10 ± 41.82) and average lipids productivity of 37.49 ± 5.26 g/m(3)/day (maximum value of 73.70 ± 9.75); simultaneously, the microalgae growth effectively removed nutrients from the wastewater, including 105.6 ± 17.1 mg CODCr/L/day, 36.8 ± 6.1mg N/L/day and 6.1 ± 1.1 mg P/L/day.
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze Water Environment for Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze Water Environment for Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
authors.forename: Li-Bin
authors.initials: LB
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze Water Environment for Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
authors.forename: Ya-Lei
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze Water Environment for Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
authors.forename: Fang-Chao
authors.initials: FC
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze Water Environment for Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China. Electronic address: chq123wd@163.com.
authors.forename: Hua-Qiang
authors.initials: HQ
authors.lastname: Chu
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze Water Environment for Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
mesh_terms: D000693:Anaerobiosis / Q000502:physiology*; D061249:Batch Cell Culture Techniques; D018533:Biomass; D002708:Chlorella / Q000254:growth & development*; D008055:Lipids; D012722:Sewage / Q000382:microbiology*; D062065:Wastewater / Q000032:analysis
chemical_list: D008055:Lipids; D012722:Sewage; D062065:Wastewater
keywords: Anaerobically digested activated sludge; Chlorella pyrenoidosa; Lipids production; Nutrients removal; Secondary-treated effluent