title: Human Mesenchymal Stem Cells Protect Dorsal Root Ganglia from the Neurotoxic Effect of Cisplatin.
abstract: OBJECTIVE
  Peripheral neurotoxicity is a dose-limiting factor of many chemotherapeutic agents, including cisplatin. Mesenchymal stem cells are promising for the treatment of several neurological disorders, and our aim was to verify the neuroprotective potential of human mesenchymal stem cells (hMSCs) on dorsal root ganglia (DRG) exposed to cisplatin.
  
  
  METHODS
  DRG were exposed to different cisplatin concentrations and then co-cultured with hMSCs or with hMSC-conditioned medium.
  
  
  RESULTS
  hMSCs showed a neuroprotective effect on cisplatin-induced death of DRG, mediated by direct contact. Moreover, DRG exhibited an MSC-dependent promotion of neurite outgrowth, in particular at early time points. For this effect, the expression of Neurite Outgrowth Inhibitor (NOGO) and Myelin Associated Glycoprotein (MAG) by hMSCs was pivotal.
  
  
  CONCLUSIONS
  hMSCs are a promising tool for reducing the neurotoxic effect of cisplatin.
authors.affiliation: Experimental Neurology Unit, Department of Surgery and Translational Medicine, Milano-Bicocca University, Monza, Italy Milan Center for Neuroscience, Milan, Italy arianna.scuteri@unimib.it.
authors.forename: Arianna
authors.initials: A
authors.lastname: Scuteri
authors.affiliation: Experimental Neurology Unit, Department of Surgery and Translational Medicine, Milano-Bicocca University, Monza, Italy.
authors.forename: Maddalena
authors.initials: M
authors.lastname: Ravasi
authors.affiliation: Experimental Neurology Unit, Department of Surgery and Translational Medicine, Milano-Bicocca University, Monza, Italy PhD Neuroscience Program, Milano-Bicocca University, Monza, Italy Milan Center for Neuroscience, Milan, Italy.
authors.forename: Marianna
authors.initials: M
authors.lastname: Monfrini
authors.affiliation: Experimental Neurology Unit, Department of Surgery and Translational Medicine, Milano-Bicocca University, Monza, Italy.
authors.forename: Azzurra
authors.initials: A
authors.lastname: Milano
authors.affiliation: M.Tettamanti" Research Center, Pediatric Clinic, Milano-Bicocca University, Monza, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: D'Amico
authors.affiliation: Experimental Neurology Unit, Department of Surgery and Translational Medicine, Milano-Bicocca University, Monza, Italy Milan Center for Neuroscience, Milan, Italy.
authors.forename: Mariarosaria
authors.initials: M
authors.lastname: Miloso
authors.affiliation: Experimental Neurology Unit, Department of Surgery and Translational Medicine, Milano-Bicocca University, Monza, Italy Milan Center for Neuroscience, Milan, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Tredici
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002945:Cisplatin / Q000633:toxicity*; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D009185:Myelin Proteins / Q000378:metabolism; D018992:Myelin-Associated Glycoprotein / Q000378:metabolism; D016501:Neurites / Q000378:metabolism; D000070798:Nogo Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D017077:Culture Media, Conditioned; C115082:MAG protein, human; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D000070798:Nogo Proteins; C000603199:RTN4 protein, human; C000603197:Rtn4 protein, rat; D002945:Cisplatin
keywords: DRG; MAG; MSC; NOGO; cisplatin; neurite elongation; peripheral neuropathy