title: Anti-plasmodial activity of ethanolic extract of root and stem back of Cassia sieberiana DC on mice.
abstract: OBJECTIVE
  This study assessed within 4 days of suppressive test in vivo antimalarial activity of Ethanolic extract of root and stem bark of Cassia sieberiana DC against chloroquine sensitive strain of Plasmodium berghei NK65 in mice.
  
  
  METHODS
  Two sets, each of five groups of four mice per each group were used. The groups of animals were administered with 100, 200, and 300 mg extract/kg body weight respectively, while positive control group were administered with 5 mg chloroquine/kg body weight and the negative control, were administered with 5 m1 distilled water/kg body weight. Oral acute toxicity was evaluated using up and down procedure.
  
  
  RESULTS
  Both the root and stem bark extract of C. sieberiana showed antimalarial property for suppressive tests. Chemo suppression of the root extract exerted significant (P < 0.05) dose-dependent reduction in the level of parasiteamia of 30.7%, 52.7%, and 55.8%. And from stem extract 17.6%, 38.0%, and 63.9% were recorded on mice when compared with 96.0% suppressive rate obtained from weight of chloroquine. The phytochemical screening of the plants root and stem bark extract revealed the presence of alkaloids, anthraquinones, flavonoids, triterpenoids, tannins, cardiac glycosides, saponins, reducing sugars and carbohydrates. The oral median lethal dose was determined to be >3000 mg/kg body weight.
  
  
  CONCLUSIONS
  The acute toxicity results of this study showed that the plant parts used are assumed to be safe and has anti-plasmodial activity that can be explored for the management of malaria.
authors.affiliation: Department of Medical Microbiology, Usmanu Danfodiyo University Sokoto, Nigeria.
authors.forename: Nuhu
authors.initials: N
authors.lastname: Abdulrazak
authors.affiliation: Department of Medical Microbiology, Usmanu Danfodiyo University Sokoto, Nigeria.
authors.forename: Umar Imam
authors.initials: UI
authors.lastname: Asiya
authors.affiliation: Department of Medical Microbiology, Usmanu Danfodiyo University Sokoto, Nigeria.
authors.forename: NataaLa Shehu
authors.initials: NS
authors.lastname: Usman
authors.affiliation: Department of chemical pathology, Usmanu Danfodiyo University Sokoto, Nigeria.
authors.forename: Iduh Micheal
authors.initials: IM
authors.lastname: Unata
authors.affiliation: Department of Microbiology and Parasitology, Usmanu Danfodiyo University Teaching Hospital Sokoto, Nigeria.
authors.forename: Aminu
authors.initials: A
authors.lastname: Farida
keywords: Anti-malaria; phytochemicals; suppressive test