title: Mitochondrial apoptosis and autophagy in the process of adipose-derived stromal cell differentiation into astrocytes.
abstract: This study aimed to investigate the phenomenon of mitochondrial apoptosis and autophagy in the process of adipose-derived stromal cell (ADSC) differentiation into astrocytes for 48 h, 7 days, 14 days, and 21 days. Immunocytochemistry and Western blotting indicated that the expression of GFAP reached a peak on the 7th day (P < 0.05). The expression of Bcl-2 was decreased (P < 0.05), but Bax, Cyt-c, and LC3 increased with time and reached a maximum on the 14th day (P < 0.05). TEM was used to observe the ultrastructure of mitochondria, apoptosis, and autophagy. An MTT assay indicated that the number of surviving cells decreased. During ADSC differentiation into astrocytes, the effect of Bcl-2 inhibition on apoptosis decreased, whereas mitochondrial apoptosis and autophagy were enhanced; however, this selective autophagy could not eliminate all the damaged mitochondria, leading to mitochondrial apoptosis.
authors.affiliation: Department of Neurology, Affiliated Kailuan General Hospital of North China University of Science and Technology, Tangshan, 063000, Hebei Province, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Neurology, Affiliated Kailuan General Hospital of North China University of Science and Technology, Tangshan, 063000, Hebei Province, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Affiliated Kailuan General Hospital of North China University of Science and Technology, Tangshan, 063000, Hebei Province, China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Neurology, Affiliated Kailuan General Hospital of North China University of Science and Technology, Tangshan, 063000, Hebei Province, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Ou
authors.affiliation: Department of Neurology, Affiliated Kailuan General Hospital of North China University of Science and Technology, Tangshan, 063000, Hebei Province, China.
authors.forename: Quanquan
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Neurology, Affiliated Kailuan General Hospital of North China University of Science and Technology, Tangshan, 063000, Hebei Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.affiliation: Department of Neurology, Affiliated Kailuan General Hospital of North China University of Science and Technology, Tangshan, 063000, Hebei Province, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Neurology, Affiliated Kailuan General Hospital of North China University of Science and Technology, Tangshan, 063000, Hebei Province, China.
authors.forename: Zanzan
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Department of Neurology, Affiliated Kailuan General Hospital of North China University of Science and Technology, Tangshan, 063000, Hebei Province, China.
authors.forename: Xuhong
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000328:Adult; D017209:Apoptosis / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D001343:Autophagy / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology*; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins
keywords: adipose tissue/adipocytes; apoptosis; cell death; cell differentiation; mitochondria