title: Monitoring of TGF-β 1-Induced Human Lung Adenocarcinoma A549 Cells Epithelial-Mesenchymal Transformation Process by Measuring Cell Adhesion Force with a Microfluidic Device.
abstract: The epithelial-mesenchymal transition (EMT) is a process in which epithelial cells lose their cell polarity and cell-cell adhesion, and gain migratory and invasive properties. It is believed that EMT is associated with initiation and completion of the invasion-metastasis cascade. In this study, an economic approach was developed to fabricate a microfluidic device with less instrumentation requirement for the investigation of EMT by quantifying cell adhesion force. Fluid shear force was precisely controlled by a homemade microfluidic perfusion apparatus and interface. The adhesion capability of the human lung adenocarcinoma cell line A549 on different types of extracellular matrix protein was studied. In addition, effects of transforming growth factor-β (TGF-β) on EMT in A549 cells were investigated by characterizing the adhesion force changes and on-chip fluorescent staining. The results demonstrate that the microfluidic device is a potential tool to characterize the epithelial-mesenchymal transition process by measuring cell adhesion force.
authors.affiliation: Central Laboratory of Yongchuan Hospital, Chongqing Medical University, Chongqing, 402160, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing, 400715, China.|Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease, Southwest University, Chongqing, 400715, China.
authors.forename: AnXiu
authors.initials: A
authors.lastname: Gao
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing, 400715, China. lingyu12@swu.edu.cn.|Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease, Southwest University, Chongqing, 400715, China. lingyu12@swu.edu.cn.
authors.forename: Ling
authors.initials: L
authors.lastname: Yu
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000502:physiology*
chemical_list: D053773:Transforming Growth Factor beta1
keywords: Cell adhesion force; Epithelial-mesenchymal transition; Microfluidic; Transforming growth factor-β