title: Taurine Protected Against the Impairments of Neural Stem Cell Differentiated Neurons Induced by Oxygen-Glucose Deprivation.
abstract: Cell transplantation of neural stem cells (NSCs) is a promising approach for neurological recovery both structurally and functionally. However, one big obstacle is to promote differentiation of NSCs into neurons and the followed maturation. In the present study, we aimed to investigate the protective effect of taurine on the differentiation of NSCs and subsequent maturation of their neuronal lineage, when exposed to oxygen-glucose deprivation (OGD). The results suggested that taurine (5-20 mM) promoted the viability and proliferation of NSCs, and it protected against 8 h of OGD induced impairments. Furthermore, 20 mM taurine promoted NSCs to differentiate into neurons after 7 days of culture, and it also protected against the suppressive impairments of 8 h of OGD. Consistently, taurine (20 mM) promoted the neurite sprouting and outgrowth of the NSC differentiated neurons after 14 days of differentiation, which were significantly inhibited by OGD (8 h). At D21, the mushroom spines and spine density were promoted or restored by 20 mM taurine. Taken together, the enhanced viability and proliferation of NSCs, more differentiated neurons and the promoted maturation of neurons by 20 mM taurine support its therapeutic application during stem cell therapy to enhance neurological recovery. Moreover, it protected against the impairments induced by OGD, which may highlight its role for a more direct therapeutic application especially in an ischemic stroke environment.
authors.affiliation: School of Biology and Basic Medical Sciences, Medical College, Soochow University, 199 Ren'ai Road, Suzhou, 215123, Jiangsu, China.|Key Laboratory for Ecology and Pollution Control of Coastal Wetlands, School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, 224051, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xiao
authors.affiliation: School of Biology and Basic Medical Sciences, Medical College, Soochow University, 199 Ren'ai Road, Suzhou, 215123, Jiangsu, China.
authors.forename: Huazhen
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Biology and Basic Medical Sciences, Medical College, Soochow University, 199 Ren'ai Road, Suzhou, 215123, Jiangsu, China.
authors.forename: Zeyun
authors.initials: Z
authors.lastname: Gu
authors.affiliation: School of Biology and Basic Medical Sciences, Medical College, Soochow University, 199 Ren'ai Road, Suzhou, 215123, Jiangsu, China.
authors.forename: Sining
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Biology and Basic Medical Sciences, Medical College, Soochow University, 199 Ren'ai Road, Suzhou, 215123, Jiangsu, China. jichenggreen@163.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Ji
mesh_terms: D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D015687:Cell Hypoxia / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D049229:Dendritic Spines / Q000187:drug effects; D005947:Glucose / Q000172:deficiency*; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology; D051379:Mice; D058953:Neural Stem Cells / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D013654:Taurine / Q000494:pharmacology*
chemical_list: D000963:Antimetabolites; D018696:Neuroprotective Agents; D013654:Taurine; D001973:Bromodeoxyuridine; D005947:Glucose
keywords: Differentiation; Neural stem cell; Neurons; Taurine; oxygen–glucose deprivation