title: Elevated microRNA-25 inhibits cell apoptosis in lung cancer by targeting RGS3.
abstract: The non-small-cell lung cancer (NSCLC) is the most common type of lung cancer that affects the human health. But, the underlying mechanisms and effective therapy are still absent. MicroRNAs (miRNAs) are small RNAs that specifically bind to the 3' untranslated region (3'UTR) of its target and regulate the protein level of the target at post-transcriptional level. A lot of miRNAs had been found abnormally regulated in the NSCLC patients, and understanding their specific roles in the pathogenesis of NSCLC will help us to develop novel therapeutic approaches. Here, we reported that miR-25 is dramatically upregulated in NSCLC tissues and negatively correlated with RGS3 protein. A conserved binding sequence in the 3'UTR of RGS3 gene to miR-25 was identified, and overexpression of miR-25 induces the RGS3 inhibition. Importantly, suppression of miR-25 facilitates the cell apoptosis and retards the cell proliferation in A549 and H520 cell lines. Our data provide a novel miR-25/RGS3 signal in the development of lung cancer.
authors.affiliation: Department of Anesthesiology, Renmin Hospital, Wuhan University, Wuhan, 430060, Hubei, People's Republic of China. chzz668899@163.com.
authors.forename: Zhize
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, Renmin Hospital, Wuhan University, Wuhan, 430060, Hubei, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Anesthesiology, Renmin Hospital, Wuhan University, Wuhan, 430060, Hubei, People's Republic of China.
authors.forename: Qingtao
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Anesthesiology, Renmin Hospital, Wuhan University, Wuhan, 430060, Hubei, People's Republic of China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Xia
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D020710:RGS Proteins / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction
chemical_list: D020413:3' Untranslated Regions; C543351:MIRN25 microRNA, human; D035683:MicroRNAs; D020710:RGS Proteins; C485898:RGS3 protein, human
keywords: Apoptosis; NSCLC; RGS3; miR-25