title: Application of gold nanoparticles produced by laser ablation for immunochromatographic assay labeling.
abstract: Nanodispersed gold is widely used as a marker in different analytical systems. For such purposes, it is usually obtained by the reduction of salts. This work studied the potential analytical applications of nanodispersed gold obtained by laser ablation because gold produced with this method has no chemical coating. The nanoparticles produced were characterized by transmission electron microscopy and spectrophotometry. The average size of the particles was 24.5 nm. Concentration dependences of antibody immobilization on ablative gold were obtained. With the use of antibody-conjugated nanoparticles, an immunochromatographic system was constructed for the detection of zearalenone mycotoxin. This immunoassay was characterized by a detection limit of 0.1 ng/ml antigen with an assay duration of only 15 min, which is on par with current test systems comprising nanodispersed gold obtained by chemical reduction. The simplicity of ablative dispersing makes this a prospective method for the labeling of various antibodies for analytical use.
authors.affiliation: A. N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
authors.forename: A E
authors.initials: AE
authors.lastname: Urusov
authors.affiliation: A. N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Petrakova
authors.affiliation: Wave Research Center of A. M. Prokhorov General Physics Institute of the Russian Academy of Sciences, 119991 Moscow, Russia.
authors.forename: P G
authors.initials: PG
authors.lastname: Kuzmin
authors.affiliation: A. N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Zherdev
authors.affiliation: Russian National Scientific Center of Molecular Diagnostics and Therapy, 117630 Moscow, Russia.
authors.forename: P G
authors.initials: PG
authors.lastname: Sveshnikov
authors.affiliation: Wave Research Center of A. M. Prokhorov General Physics Institute of the Russian Academy of Sciences, 119991 Moscow, Russia; National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 115409 Moscow, Russia.
authors.forename: G A
authors.initials: GA
authors.lastname: Shafeev
authors.affiliation: A. N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia. Electronic address: dzantiev@inbi.ras.ru.
authors.forename: B B
authors.initials: BB
authors.lastname: Dzantiev
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D002846:Chromatography, Affinity; D006046:Gold / Q000737:chemistry*; D007834:Lasers; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D015025:Zearalenone / Q000032:analysis* / Q000276:immunology
chemical_list: D055910:Antibodies, Immobilized; D015025:Zearalenone; D006046:Gold
keywords: Gold nanoparticles; Immunochromatography; Laser ablation; Mycotoxin; Zearalenone