title: Identification, characterization and expression profiling of the Tollip gene in Yesso scallop (Patinopecten yessoensis).
abstract: Toll-interacting protein (Tollip) is a critical regulator of Toll-like receptor (TLR)-mediated innate immune responses. However, the Tollip gene has not been systematically characterized in shellfish. In this study, we identified and characterized a Tollip gene, PyTollip, in Yesso scallop (Patinopecten yessoensis). Phylogenetic and protein structural analyses were conducted to determine its sequence identities and evolutionary relationships. Compared with Tollip genes from other invertebrate and vertebrate species, the PyTollip gene is highly conserved in its sequence and structural features, except that a unique asparagine residue was found at a conserved site in the C2 domain of PyTollip. Quantitative real-time PCR was used to investigate the expression profiles of PyTollip in different developmental stages, healthy adult tissues, and in hemolymph after Micrococcus luteus and Vibrio anguillarum bacterial infection. Real-time PCR analysis demonstrated differential expression of PyTollip at the acute phase (3 h) after infection with Gram-negative (V. anguillarum) and Gram-positive (M. luteus) bacteria. A second strong response of PyTollip expression was observed 24 h after challenge with V. anguillarum. Collectively, these results provide novel insights into the specific role and response of Tollip and TLR signaling pathways in host immune responses against different bacterial pathogens in bivalves.
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China.
authors.forename: Ru
authors.initials: R
authors.lastname: Zhang
authors.forename: Ruojiao
authors.initials: R
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Shuyue
authors.initials: S
authors.lastname: Wang
authors.forename: Mengran
authors.initials: M
authors.lastname: Zhang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Hu
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhang
authors.forename: Shi
authors.initials: S
authors.lastname: Wang
authors.forename: Ruijia
authors.initials: R
authors.lastname: Wang
authors.forename: Zhenmin
authors.initials: Z
authors.lastname: Bao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D020869:Gene Expression Profiling; D006458:Hemolymph / Q000378:metabolism / Q000382:microbiology; D054884:Host-Pathogen Interactions; D007113:Immunity, Innate; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016982:Micrococcus luteus / Q000276:immunology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D049897:Pectinidae / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D010802:Phylogeny; D015398:Signal Transduction; D051193:Toll-Like Receptors / Q000378:metabolism; D059467:Transcriptome; D014733:Vibrio / Q000276:immunology
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D051193:Toll-Like Receptors