title: Ultrastructural features of collagen in thyroid carcinoma tissue observed by polarization second harmonic generation microscopy.
abstract: Changes in collagen ultrastructure between malignant and normal human thyroid tissue were investigated ex vivo using polarization second harmonic generation (SHG) microscopy. The second-order nonlinear optical susceptibility tensor component ratio and the degree of linear polarization (DOLP) of the SHG signal were measured. The ratio values are related to the collagen ultrastructure, while DOLP indicates the relative amount of coherent signal and incoherent scattering of SHG. Increase in ratio values and decrease in DOLP were observed for tumor tissue compared to normal thyroid, indicating higher ultrastructural disorder in tumor collagen.
authors.affiliation: Princess Margaret Cancer Centre, University Health Network, 101 College Street, Toronto, ON M5G 1L7, Canada.
authors.forename: Danielle
authors.initials: D
authors.lastname: Tokarz
authors.affiliation: Department of Physics, Institute for Optical Sciences and Department of Physical and Chemical Sciences, University of Toronto, 3359 Mississauga Road North, Mississauga, ON L5L 1C6, Canada.
authors.forename: Richard
authors.initials: R
authors.lastname: Cisek
authors.affiliation: Department of Physics, Institute for Optical Sciences and Department of Physical and Chemical Sciences, University of Toronto, 3359 Mississauga Road North, Mississauga, ON L5L 1C6, Canada.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Golaraei
authors.affiliation: University Health Network/University of Toronto, 200 Elizabeth Street, Toronto, ON M5G 2C4, Canada.
authors.forename: Sylvia L
authors.initials: SL
authors.lastname: Asa
authors.affiliation: Department of Physics, Institute for Optical Sciences and Department of Physical and Chemical Sciences, University of Toronto, 3359 Mississauga Road North, Mississauga, ON L5L 1C6, Canada ; virgis.barzda@utoronto.ca.
authors.forename: Virginijus
authors.initials: V
authors.lastname: Barzda
authors.affiliation: Princess Margaret Cancer Centre, University Health Network, 101 College Street, Toronto, ON M5G 1L7, Canada.
authors.forename: Brian C
authors.initials: BC
authors.lastname: Wilson
keywords: (170.3880) Medical and biological imaging; (170.4730) Optical pathology; (170.6935) Tissue characterization; (180.4315) Nonlinear microscopy