title: Effect of Preexposure to Triazoles on Susceptibility and Virulence of Rhizopus oryzae.
abstract: Triazole prophylaxis has become the norm in patients with hematological malignancies. Breakthrough infections caused by Mucorales during triazole prophylaxis remain a challenging problem. We found that preexposure of Rhizopus oryzae to antifungal triazoles (fluconazole, voriconazole, posaconazole, and itraconazole) did not modify the in vitro susceptibility of Rhizopus oryzae to posaconazole. In contrast, preexposure of Rhizopus to triazoles was associated with the enhanced in vitro susceptibility of R. oryzae to amphotericin B. Preexposure to posaconazole did not alter the virulence of R. oryzae in the fly model of mucormycosis.
authors.affiliation: Department of Infectious Diseases, MD Anderson Cancer Center, Houston, Texas, USA Chrono-Environment UMR 6249 Research Team, Franche-Comté University and Parasitology-Mycology Department, Central University Hospital (CHU), Besançon, France.
authors.forename: Anne-Pauline
authors.initials: AP
authors.lastname: Bellanger
authors.affiliation: Department of Infectious Diseases, MD Anderson Cancer Center, Houston, Texas, USA.
authors.forename: Nathaniel D
authors.initials: ND
authors.lastname: Albert
authors.affiliation: Department of Medical Science and Surgery, University Orsola-Malpighi, Bologna, Italy.
authors.forename: Russell E
authors.initials: RE
authors.lastname: Lewis
authors.affiliation: Transplantation-Oncology Infectious Diseases Program and Departments of Medicine, Pediatrics, and Microbiology and Immunology, Weill Cornell Medical Center, New York, New York, USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Walsh
authors.affiliation: Department of Infectious Diseases, MD Anderson Cancer Center, Houston, Texas, USA dkontoyi@mdanderson.org.
authors.forename: Dimitrios P
authors.initials: DP
authors.lastname: Kontoyiannis
mesh_terms: D000362:Agar; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D004195:Disease Models, Animal; D004331:Drosophila melanogaster / Q000187:drug effects / Q000382:microbiology; D005260:Female; D015725:Fluconazole / Q000494:pharmacology*; D017964:Itraconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009091:Mucormycosis / Q000188:drug therapy / Q000382:microbiology / Q000401:mortality; D012233:Rhizopus / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D016019:Survival Analysis; D014230:Triazoles / Q000494:pharmacology*; D014774:Virulence; D065819:Voriconazole / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D014230:Triazoles; D017964:Itraconazole; C101425:posaconazole; D015725:Fluconazole; D000362:Agar; D065819:Voriconazole