title: Quantitative SERS studies by combining LOC-SERS with the standard addition method.
abstract: Here, we report on a proof-of-concept study highlighting a new approach for quantitative surface enhanced Raman spectroscopy (SERS) measurements. This has been achieved by implementing the standard addition method (SAM) within a lab-on-a-chip (LOC) system. The approach has been successfully tested to quantify congo red as a model analyte even in the presence of the chemically related molecule methyl red. Thus, the developed concept demonstrates its potential to quantify analytes via SERS in the presence of other SERS active molecules. Graphical Abstract Congo red was quantified by means of the standard addition method implemented in the lab-on-a-chip device. Due to the developed approach, a direct detection out of the sample and in the presence of an interfering substance was possible.
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.|Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Str. 9, 07745, Jena, Germany.|InfectoGnostics Forschungscampus Jena e.V., Zentrum für Angewandte Forschung, Philosophenweg 7, 07743, Jena, Germany.
authors.forename: Evelyn
authors.initials: E
authors.lastname: Kämmer
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.
authors.forename: Konstanze
authors.initials: K
authors.lastname: Olschewski
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.|InfectoGnostics Forschungscampus Jena e.V., Zentrum für Angewandte Forschung, Philosophenweg 7, 07743, Jena, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Stöckel
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.|InfectoGnostics Forschungscampus Jena e.V., Zentrum für Angewandte Forschung, Philosophenweg 7, 07743, Jena, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Rösch
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.|Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Str. 9, 07745, Jena, Germany.|InfectoGnostics Forschungscampus Jena e.V., Zentrum für Angewandte Forschung, Philosophenweg 7, 07743, Jena, Germany.
authors.forename: Karina
authors.initials: K
authors.lastname: Weber
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.|Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Str. 9, 07745, Jena, Germany.|InfectoGnostics Forschungscampus Jena e.V., Zentrum für Angewandte Forschung, Philosophenweg 7, 07743, Jena, Germany.
authors.forename: Dana
authors.initials: D
authors.lastname: Cialla-May
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.|Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Str. 9, 07745, Jena, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bocklitz
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany. juergen.popp@uni-jena.de.|Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Str. 9, 07745, Jena, Germany. juergen.popp@uni-jena.de.|InfectoGnostics Forschungscampus Jena e.V., Zentrum für Angewandte Forschung, Philosophenweg 7, 07743, Jena, Germany. juergen.popp@uni-jena.de.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
mesh_terms: D001391:Azo Compounds / Q000032:analysis*; D004396:Coloring Agents / Q000032:analysis*; D003224:Congo Red / Q000032:analysis*; D004867:Equipment Design; D056656:Lab-On-A-Chip Devices; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D012834:Silver; D003224:Congo Red; C008492:methyl red
keywords: Congo red; Lab-on-a-chip; SERS; Standard addition method