title: Resonance light scattering detection of fructose bisphosphates using uranyl-salophen complex-modified gold nanoparticles as optical probe.
abstract: In this paper, we report a resonance light scattering (RLS) method for the determination of fructose bisphosphates (FBPs) in water solution using fructose 1,6-bisphosphate (F-1,6-BP) as a model analyte without the procedure of extracting target analyte. The method used a type of modified gold nanoparticles (GNPs) as optical probe. The modified GNPs are uranyl-salophen-cysteamine-GNPs (U-Sal-Cy-GNPs) which are obtained through the acylation reaction of carboxylated salophen with cysteamine-capped GNPs (Cy-GNPs) to form Sal-Cy-GNPs and then the chelation reaction of uranyl with tetradentate ligand salophen in the Sal-Cy-GNPs. A FBP molecule is used easily to connect two U-Sal-Cy-GNPs to cause the aggregation of the GNPs by utilizing the specific affinity of uranyl-salophen complex to phosphate group, resulting in the production of strong RLS signal from the system. The amount of FBPs can be determined through detecting the RLS intensity change of the system. A linear range was found to be 2.5 to 75 nmol/L with a detection limit of 0.91 nmol/L under optimal conditions. The method has been successfully used to determine FBPs in real samples with the recoveries of 96.5-103.5 %.
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan, 421001, China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan, 421001, China. llfcxllc@163.com.
authors.forename: Lifu
authors.initials: L
authors.lastname: Liao
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan, 421001, China.
authors.forename: Rurong
authors.initials: R
authors.lastname: Wu
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan, 421001, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan, 421001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan, 421001, China.
authors.forename: Xilin
authors.initials: X
authors.lastname: Xiao
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan, 421001, China.
authors.forename: Changming
authors.initials: C
authors.lastname: Nie
mesh_terms: D000067493:Dynamic Light Scattering; D005635:Fructosediphosphates / Q000032:analysis / Q000652:urine*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009942:Organometallic Compounds / Q000737:chemistry*
chemical_list: D005635:Fructosediphosphates; D009942:Organometallic Compounds; C518147:uranyl salophen; D006046:Gold; C029063:fructose-1,6-diphosphate
keywords: Fructose bisphosphates; Gold nanoparticles; Resonance light scattering; Salophen; Uranyl