title: Oral microbiome in chinese patients with aggressive periodontitis and their family members.
abstract: OBJECTIVE
  To investigate the microbiome composition in Chinese patients with aggressive periodontitis (AgP), and to compare the similarity of bacterial profiles between AgP patients and their family members.
  
  
  METHODS
  Pooled subgingival plaque and saliva samples were collected from 10 AgP patients and 10 of their first-degree blood relatives with chronic periodontitis. DNA amplicons of the V1-V3 hypervariable region of the bacterial 16S rRNA gene were generated, and were subjected to 454-pyrosequencing.
  
  
  RESULTS
  In subgingival plaque, the unweighted UniFrac distances between family members were significantly lower than those in unrelated participants (p = 0.039). Compared with the relatives, the microbiota of subgingival plaque and saliva from AgP patients revealed significantly lower taxonomic diversity. High relative abundance of Porphyromonas gingivalis (about 35.88%) was detected in subgingival plaque from AgP patients. The relative abundance of P. gingivalis and Red complex pathogens (P. gingivalis, Treponema denticola and Tannerella forsythia) in the subgingival plaque and saliva samples from the same individual were significantly correlated in AgP patients (ρ= 0.687 and 0.678, respectively).
  
  
  CONCLUSIONS
  There is a kinship in the phylogenetic architecture of microbiota among Chinese AgP patients and their family members. P. gingivalis might be a predominant pathogen in these Chinese AgP patients.
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Xianghui
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Lu
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Shi
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Xiane
authors.initials: X
authors.lastname: Wang
authors.affiliation: Central laboratory, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: BGI, Beishan Industrial Zone, Guangdong, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Huanxin
authors.initials: H
authors.lastname: Meng
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans; D010520:Aggressive Periodontitis; D001439:Bacteroides; D003773:Dental Plaque; D006801:Humans; D064307:Microbiota; D010518:Periodontitis; D010802:Phylogeny; D016966:Porphyromonas gingivalis; D012336:RNA, Ribosomal, 16S; D048373:Treponema denticola
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: aggressive periodontitis; family; microbiome; pyrosequencing