title: Quantification of polyphenols and flavonoid content and evaluation of anti-inflammatory and antimicrobial activities of Stenocereus stellatus extracts.
abstract: The hexanic, ethyl acetate and methanolic extracts from branches of Stenocereus stellatus were tested in both the 12-O-tetradecanoylphorbol-13-acetate (TPA) - induced ear oedema model and antimicrobial activity assay. The % of oedema inhibition, the Minimum Inhibitory Concentration (MIC), as well as the polyphenolic and flavonoid content were determined. Also, extracts were analysed by gas chromatography-mass spectrometry (GC-MS). In TPA model, the three extracts showed moderate oedema inhibition. In the antimicrobial activity assay, methanolic extract shows better MIC against all strains. The lowest MICs were for Candida albicans (31 μg/mL) and Rhizopus sp. (15 μg/mL). Also, 50.78 mg eq. of gallic acid/g extract of polyphenol and 115.12 mg eq. of catequine/g extract of flavonoids content were founded in ethyl acetate extract. In the chromatographic analysis, β-sitosterol, β-amyrine, betulin and some other molecules were identified. The results show that S. stellatus possess antimicrobial activities against some fungus species.
authors.affiliation: a Facultad de Ciencias Químicas , Universidad La Salle , México City , Mexico.
authors.forename: Diego
authors.initials: D
authors.lastname: Soto-Cabrera
authors.affiliation: a Facultad de Ciencias Químicas , Universidad La Salle , México City , Mexico.
authors.forename: Juan R
authors.initials: JR
authors.lastname: Salazar
authors.affiliation: a Facultad de Ciencias Químicas , Universidad La Salle , México City , Mexico.
authors.forename: Inés
authors.initials: I
authors.lastname: Nogueda-Gutiérrez
authors.affiliation: a Facultad de Ciencias Químicas , Universidad La Salle , México City , Mexico.
authors.forename: Mariana
authors.initials: M
authors.lastname: Torres-Olvera
authors.affiliation: a Facultad de Ciencias Químicas , Universidad La Salle , México City , Mexico.
authors.forename: Anabelle
authors.initials: A
authors.lastname: Cerón-Nava
authors.affiliation: a Facultad de Ciencias Químicas , Universidad La Salle , México City , Mexico.
authors.forename: Juan
authors.initials: J
authors.lastname: Rosales-Guevara
authors.affiliation: b Departamento de Botánica, Instituto de Biología , Universidad Nacional Autónoma de México, Ciudad Universitaria , México City , Mexico.
authors.forename: Teresa
authors.initials: T
authors.lastname: Terrazas
authors.affiliation: c Facultad de Estudios Superiores Zaragoza , Universidad Nacional Autónoma de México , México City , Mexico.
authors.forename: Hortensia
authors.initials: H
authors.lastname: Rosas-Acevedo
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry; D029421:Cactaceae / Q000737:chemistry* / Q000378:metabolism; D002176:Candida albicans / Q000187:drug effects; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005419:Flavonoids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D008826:Microbial Sensitivity Tests; D010703:Phorbol Esters / Q000633:toxicity; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D059808:Polyphenols / Q000032:analysis*; D012233:Rhizopus / Q000187:drug effects; D064210:Secondary Metabolism; D012855:Sitosterols / Q000032:analysis
chemical_list: D000890:Anti-Infective Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D005419:Flavonoids; D010703:Phorbol Esters; D010936:Plant Extracts; D059808:Polyphenols; D012855:Sitosterols; C025473:gamma-sitosterol; C000600213:12-O-undecadienoylphorbol-13-acetate
keywords: Betulin; Candidda albicans; Rhizopus sp.; Stenocereus stellatus; anti-inflammatory; antimicrobial