title: Enrichment and identification of Δ(9)-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants.
abstract: This data article refers to the report Δ(9)-Tetrahydrocannabinolic acid synthase (THCAS) production in Pichia pastoris enables chemical synthesis of cannabinoids (Lange et. al. 2015) [2]. THCAS was produced on a 2 L lab scale using recombinant P. pastoris KM71 KE1. Enrichment of THCAS as a technically pure enzyme was realized using dialysis and cationic exchange chromatography. nLC-ESI-MS/MS analysis identified THCAS in different fractions obtained by cationic exchange chromatography.
authors.affiliation: Laboratory of Chemical Biotechnology, Department of Biochemical & Chemical Engineering, TU Dortmund University, 44227 Dortmund, Germany ; Department of Solar Materials, Helmholtz Centre for Environmental Research (UFZ), 04318 Leipzig, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Lange
authors.affiliation: Plant Biochemistry, Ruhr University Bochum, 44801 Bochum, Germany.
authors.forename: Ansgar
authors.initials: A
authors.lastname: Poetsch
authors.affiliation: Department of Solar Materials, Helmholtz Centre for Environmental Research (UFZ), 04318 Leipzig, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schmid
authors.affiliation: Laboratory of Chemical Biotechnology, Department of Biochemical & Chemical Engineering, TU Dortmund University, 44227 Dortmund, Germany.
authors.forename: Mattijs K
authors.initials: MK
authors.lastname: Julsing