title: Phytochemical, antioxidant and antidiabetic evaluation of eight Bauhinia L. species from Egypt using UHPLC-PDA-qTOF-MS and chemometrics.
abstract: Bauhinia L. (Fabaceae) comprises ca. 300-350 plant species, many of which are traditionally used in folk medicine for their antidiabetic, antioxidant and anti-inflammatory effects. Bauhinia s.l. recently has been subdivided into 9 genera based on phylogenetic data: Bauhinia s.str., Barklya, Brenierea, Gigasiphon, Lysiphyllum, Phanera, Piliostigma, Schnella (American Phanera) and Tylosema. The aerial parts of 8 species corresponding to 5 genera were analyzed: Bauhinia forficata, Bauhinia variegata, B. variegata var. candida, Bauhinia galpinii, Schnella glabra, Piliostigma racemosa, Phanera vahlii and Lysiphyllum hookeri. Leaves and shoots were subjected to metabolite profiling via UHPLC-PDA-qTOF-MS coupled to multivariate data analyzes to identify compound compositional differences. A total of 90 metabolites were identified including polyphenols and fatty acids; flavonoid conjugates accounted for most of the metabolite variation observed. This study provides a comprehensive map of polyphenol composition in Bauhinia and phytochemical species aggregations are consistent with recent Bauhinia genus taxonomic relationship derived from phylogenetic studies. DPPH radical scavenging and α-glucosidase inhibitory assays were also performed to assess selected aspects of the antioxidant and antidiabetic potential for the examined species with respect to metabolite profiles.
authors.affiliation: Pharmacognosy Department, College of Pharmacy, Cairo University, 11562 Cairo, Egypt. Electronic address: mfarag73@yahoo.com.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Farag
authors.affiliation: Pharmacognosy Department, College of Pharmacy, Cairo University, 11562 Cairo, Egypt.
authors.forename: Sarah T
authors.initials: ST
authors.lastname: Sakna
authors.affiliation: Pharmacognosy Department, College of Pharmacy, Cairo University, 11562 Cairo, Egypt.
authors.forename: Nabaweya M
authors.initials: NM
authors.lastname: El-Fiky
authors.affiliation: Pharmacognosy Department, College of Pharmacy, Cairo University, 11562 Cairo, Egypt.
authors.forename: Marawan M
authors.initials: MM
authors.lastname: Shabana
authors.affiliation: Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany.
authors.forename: Ludger A
authors.initials: LA
authors.lastname: Wessjohann
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D036481:Bauhinia / Q000737:chemistry* / Q000235:genetics*; D004534:Egypt; D005227:Fatty Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008519:Medicine, Traditional; D010802:Phylogeny; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis; D044945:Proanthocyanidins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000520:alpha-Glucosidases / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D005227:Fatty Acids; D005419:Flavonoids; D007004:Hypoglycemic Agents; D059808:Polyphenols; D044945:Proanthocyanidins; D000520:alpha-Glucosidases
keywords: Bauhinia; Chemotaxonomy; Flavonoids; Multivariate data analysis; UHPLC–MS; α-Glucosidase inhibition