title: PGE2 Elevates IL-23 Production in Human Dendritic Cells via a cAMP Dependent Pathway.
abstract: PGE2 elevates IL-23 production in mouse dendritic cells while inhibits IL-23 production in isolated human monocytes. Whether this differential effect of PGE2 on IL-23 production is cell-type- or species-specific has not been investigated in detail. The present study was designed to investigate the effect of PGE2 on IL-23 production in human DCs and the possible underlying mechanisms. Human monocytes derived dendritic cells (Mo-DCs) were pretreated with or without PGE2. Then the cells were incubated with zymosan. Our results demonstrated that PGE2 promoted zymosan-induced IL-23 production in a concentration dependent manner. In addition, it was found that PGE2 is also able to elevate MyD88-mediated IL-23 p19 promoter activity. More importantly, ELISA data demonstrated that db-cAMP, a cAMP analog, and forskolin, an adenylate cyclase activator, can mimic the effect of PGE2 on zymosan-induced IL-23 production, and rp-cAMP, a protein kinase A (PKA) inhibitor, can block the effect of PGE2. Moreover, PGE2 can increase zymosan-induced expression of the mRNA levels of both p19 and p40 subunits, which was mimicked by db-cAMP and forskolin. Our data suggest that PGE2 elevates the production of IL-23 in human Mo-DCs via a cAMP dependent pathway.
authors.affiliation: Department of Cardiology, The 306th Hospital, The Chinese People's Liberation Army, Beijing 100101, China.
authors.forename: Quanxing
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Department of Cardiology, The 306th Hospital, The Chinese People's Liberation Army, Beijing 100101, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Department of Cardiology, The 306th Hospital, The Chinese People's Liberation Army, Beijing 100101, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, The 306th Hospital, The Chinese People's Liberation Army, Beijing 100101, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
authors.affiliation: CAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Haisheng
authors.initials: H
authors.lastname: Yu
authors.affiliation: CAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiangyun
authors.initials: X
authors.lastname: Yin
authors.affiliation: CAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Liguo
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The 306th Hospital, The Chinese People's Liberation Army, Beijing 100101, China.
authors.forename: Shouli
authors.initials: S
authors.lastname: Wang
mesh_terms: D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism*; D015232:Dinoprostone / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D053759:Interleukin-23 / Q000235:genetics / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015054:Zymosan / Q000494:pharmacology
chemical_list: D053759:Interleukin-23; D015054:Zymosan; D000242:Cyclic AMP; D015232:Dinoprostone