title: Eukaryotic Ribosome Assembly and Nuclear Export.
abstract: Accurate translation of the genetic code into functional polypeptides is key to cellular growth and proliferation. This essential process is carried out by the ribosome, a ribonucleoprotein complex of remarkable size and intricacy. Although the structure of the mature ribosome has provided insight into the mechanism of translation, our knowledge regarding the assembly, quality control, and intracellular targeting of this molecular machine is still emerging. Assembly of the eukaryotic ribosome begins in the nucleolus and requires more than 350 conserved assembly factors, which transiently associate with the preribosome at specific maturation stages. After accomplishing their tasks, early-acting assembly factors are released, preparing preribosomes for nuclear export. Export competent preribosomal subunits are transported through nuclear pore complexes into the cytoplasm, where they undergo final maturation steps, which are closely connected to quality control, before engaging in translation. In this chapter, we focus on the final events that commit correctly assembled ribosomal subunits for translation.
authors.affiliation: Institute of Biochemistry (IBC), Department of Biology (D-BIOL), ETH Zurich, Zurich, Switzerland; Molecular Life Science (MLS) Graduate School, Zurich, Switzerland.
authors.forename: Purnima
authors.initials: P
authors.lastname: Nerurkar
authors.affiliation: Institute of Biochemistry (IBC), Department of Biology (D-BIOL), ETH Zurich, Zurich, Switzerland; Molecular Life Science (MLS) Graduate School, Zurich, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Altvater
authors.affiliation: Institute of Biochemistry (IBC), Department of Biology (D-BIOL), ETH Zurich, Zurich, Switzerland; Biomolecular Structure and Mechanism (BSM) Graduate School, Zurich, Switzerland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Gerhardy
authors.affiliation: Institute of Biochemistry (IBC), Department of Biology (D-BIOL), ETH Zurich, Zurich, Switzerland; Molecular Life Science (MLS) Graduate School, Zurich, Switzerland.
authors.forename: Sabina
authors.initials: S
authors.lastname: Schütz
authors.affiliation: Institute of Biochemistry (IBC), Department of Biology (D-BIOL), ETH Zurich, Zurich, Switzerland.
authors.forename: Ute
authors.initials: U
authors.lastname: Fischer
authors.affiliation: Institute of Biochemistry (IBC), Department of Biology (D-BIOL), ETH Zurich, Zurich, Switzerland.
authors.forename: Christine
authors.initials: C
authors.lastname: Weirich
authors.affiliation: Institute of Biochemistry (IBC), Department of Biology (D-BIOL), ETH Zurich, Zurich, Switzerland.
authors.forename: Vikram Govind
authors.initials: VG
authors.lastname: Panse
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000378:metabolism*; D006801:Humans; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D012269:Ribosomal Proteins
keywords: 40S subunits; 60S subunits; Biogenesis; Eukaryotic cells; Nuclear transport; Ribosome