title: Simultaneous quantification of salivary 3-hydroxybutyrate, 3-hydroxyisobutyrate, 3-hydroxy-3-methylbutyrate, and 2-hydroxybutyrate as possible markers of amino acid and fatty acid catabolic pathways by LC-ESI-MS/MS.
abstract: We have developed a highly sensitive and specific method for quantification of salivary 3-hydroxybutyrate (3HB), 3-hydroxyisobutyrate (3HIB), 3-hydroxy-3-methylbutyrate (3HMB) and 2-hydroxybutyrate (2HB), which could be new non-invasive biomarkers for catabolic pathways of fatty acids/ketogenic amino acids, valine, leucine, and methionine/threonine/α-ketobutyrate, respectively. The four hydroxybutyrates (3HB, 3HIB, 3HMB, and 2HB) were extracted from 5 µl of saliva, converted to 2-pyridylmethyl (2PM) ester derivatives, and measured by liquid chromatography-tandem mass spectrometry in positive electrospray ionization mode. [(13)C4]3HB was used as an internal standard. The detection limits for the 2PM esters were <1 pg (7.9-9.6 fmol) on-column (signal-to-noise ratio = 3). Reproducibilities and recoveries of the hydroxybutyrates were validated according to one-way layout and polynomial equation, respectively. The variances between sample preparations and between measurements were calculated to be 0.45-5.28 and 0.54-3.45 %, respectively. Experiments performed using 5 µl of saliva spiked with 3.8-154.4 pmol of the four hydroxybutyrates gave recoveries of 98.5 to 108.8 %, with a mean recovery of 104.1 %. In vitro experiments in hepatocytes or skeletal muscle cells showed that addition of palmitic acid, valine, leucine or α-ketobutyrate to culture medium markedly increased the targeted hydroxybutyrate concentrations. The salivary concentration of each targeted hydroxybutyrate was positively correlated with that in serum, and the salivary levels were elevated in patients with liver cirrhosis, which is characterized by upregulated catabolism of lipids and amino acids. The proposed method is useful for quantification of salivary 3HB, 3HIB, 3HMB, and 2HB for monitoring of catabolic activities of amino acids and fatty acids.
authors.affiliation: Joint Research Center, Tokyo Medical University Ibaraki Medical Center, Ami, Japan.
authors.forename: Teruo
authors.initials: T
authors.lastname: Miyazaki
authors.affiliation: Joint Research Center, Tokyo Medical University Ibaraki Medical Center, Ami, Japan ; Division of Gastroenterology and Hepatology, Department of Internal Medicine, Tokyo Medical University Ibaraki Medical Center, 3-20-1, Chuo, Ami, Inashiki, Ibaraki 300-0395 Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Honda
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Tokyo Medical University Ibaraki Medical Center, 3-20-1, Chuo, Ami, Inashiki, Ibaraki 300-0395 Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ikegami
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Tokyo Medical University Ibaraki Medical Center, 3-20-1, Chuo, Ami, Inashiki, Ibaraki 300-0395 Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Iwamoto
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Tokyo Medical University Ibaraki Medical Center, 3-20-1, Chuo, Ami, Inashiki, Ibaraki 300-0395 Japan.
authors.forename: Tadakuni
authors.initials: T
authors.lastname: Monma
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Tokyo Medical University Ibaraki Medical Center, 3-20-1, Chuo, Ami, Inashiki, Ibaraki 300-0395 Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hirayama
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Tokyo Medical University Ibaraki Medical Center, 3-20-1, Chuo, Ami, Inashiki, Ibaraki 300-0395 Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Saito
authors.affiliation: Laboratory of Analytical Chemistry, Department of Kampo Pharmacy, Yokohama University of Pharmacy, Yokohama, Kanagawa Japan.
authors.forename: Kouwa
authors.initials: K
authors.lastname: Yamashita
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Tokyo Medical University Ibaraki Medical Center, 3-20-1, Chuo, Ami, Inashiki, Ibaraki 300-0395 Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Matsuzaki