title: Responsivity to PGE2 labor induction involves concomitant differential prostaglandin E receptor gene expression in cervix and myometrium.
abstract: Prostaglandin E2 (dinoprostone) is largely used for labor induction. However, one-third of patients do not respond to treatment. One cause of this poor response may be associated with changes in regulation of prostaglandin E receptors (EP1-4). In this study, we investigated EP mRNA expression in the uterine cervix and lower uterine segment myometrium for term births. Biopsies were obtained from women with successful (responders) and failed (non-responders) dinoprostone labor induction, while women that underwent spontaneous labor were included as controls. EP1 mRNA was upregulated in the cervical tissue of women who did not respond to dinoprostone induction. In addition, in the myometrium, significantly higher levels of EP3 mRNA were observed in women treated with dinoprostone, independent of their responsiveness. Dinoprostone-responders presented 3.6-fold higher levels of EP3 mRNA expression than the spontaneous labor group. Significantly higher levels of EP3 mRNA in the myometrium of the dinoprostone-treated group indicated that dinoprostone may regulate the EP3 gene on the transcriptional level. These results highlight the relationship between EP gene expression and delivery and indicate that understanding the regulation of prostaglandin E receptors may lead to improved labor induction.
authors.affiliation: Departamento de Ginecologia e Obstetrícia, Faculdade de Medicina, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil cristine.ufsm@gmail.com.
authors.forename: C K
authors.initials: CK
authors.lastname: Konopka
authors.affiliation: Laboratório de Biotecnologia e Reprodução Animal, Departamento de Clínica de Grandes Animais.
authors.forename: W G
authors.initials: WG
authors.lastname: Glanzner
authors.affiliation: Laboratório de Biotecnologia e Reprodução Animal, Departamento de Clínica de Grandes Animais.
authors.forename: M L
authors.initials: ML
authors.lastname: Rigo
authors.affiliation: Laboratório de Biotecnologia e Reprodução Animal, Departamento de Clínica de Grandes Animais.
authors.forename: M T
authors.initials: MT
authors.lastname: Rovani
authors.affiliation: Laboratório de Biotecnologia e Reprodução Animal, Departamento de Clínica de Grandes Animais.
authors.forename: F V
authors.initials: FV
authors.lastname: Comim
authors.affiliation: Laboratório de Biotecnologia e Reprodução Animal, Departamento de Clínica de Grandes Animais.
authors.forename: P B D
authors.initials: PB
authors.lastname: Gonçalves
authors.affiliation: Departamento de Ginecologia e Obstetrícia, Faculdade de Medicina, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: E N
authors.initials: EN
authors.lastname: Morais
authors.affiliation: Laboratório de Biotecnologia e Reprodução Animal, Departamento de Clínica de Grandes Animais.
authors.forename: A Q
authors.initials: AQ
authors.lastname: Antoniazzi
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: C F
authors.initials: CF
authors.lastname: Mello
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: I B M
authors.initials: IB
authors.lastname: Cruz
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002584:Cervix Uteri / Q000187:drug effects / Q000378:metabolism; D015232:Dinoprostone / Q000627:therapeutic use*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007751:Labor, Induced / Q000379:methods*; D009215:Myometrium / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D058306:Receptors, Prostaglandin E, EP1 Subtype / Q000096:biosynthesis / Q000235:genetics*; D058307:Receptors, Prostaglandin E, EP2 Subtype / Q000096:biosynthesis / Q000235:genetics; D058308:Receptors, Prostaglandin E, EP3 Subtype / Q000096:biosynthesis / Q000235:genetics; D017211:Treatment Failure; D014590:Uterine Contraction / Q000187:drug effects*
chemical_list: C545635:PTGER1 protein, human; C545644:PTGER2 protein, human; C545647:PTGER3 protein, human; D012333:RNA, Messenger; D058306:Receptors, Prostaglandin E, EP1 Subtype; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058308:Receptors, Prostaglandin E, EP3 Subtype; D015232:Dinoprostone