title: Rapid antimicrobial susceptibility testing of clinical isolates by digital time-lapse microscopy.
abstract: Rapid antimicrobial susceptibility testing (AST) is essential for early and appropriate therapy. Methods with short detection time enabling same-day treatment optimisation are highly favourable. In this study, we evaluated the potential of a digital time-lapse microscope system, the oCelloScope system, to perform rapid AST. The oCelloScope system demonstrated a very high accuracy (96% overall agreement) when determining the resistance profiles of four reference strains, nine clinical isolates, including multi-drug-resistant isolates, and three positive blood cultures. AST of clinical isolates (168 antimicrobial agent-organism combinations) demonstrated 3.6% minor, no major and 1.2% very major errors of the oCelloScope system compared to conventional susceptibility testing, as well as a rapid and correct phenotypic detection of strains with methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum β-lactamase (ESBL) profiles. The net average time-to-result was 108 min, with 95% of the results being available within 180 min. In conclusion, this study strongly indicates that the oCelloScope system holds considerable potential as an accurate and sensitive AST method with short time-to-result, enabling same-day targeted antimicrobial therapy, facilitating antibiotic stewardship and better patient management. A full-scale validation of the oCelloScope system including more isolates is necessary to assess the impact of using it for AST.
authors.affiliation: Department of Animal Science, Faculty of Science and Technology, Aarhus University, Blichers Allé 20, 8830, Tjele, Denmark. Marlene.Fredborg@anis.au.dk.|Department of Clinical Microbiology, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200, Aarhus, Denmark. Marlene.Fredborg@anis.au.dk.
authors.forename: M
authors.initials: M
authors.lastname: Fredborg
authors.affiliation: Department of Clinical Microbiology, Vejle Hospital, Kabbeltoft 25, 7100, Vejle, Denmark.
authors.forename: F S
authors.initials: FS
authors.lastname: Rosenvinge
authors.affiliation: Philips BioCell, Gydevang 42, 3450, Allerød, Denmark.
authors.forename: E
authors.initials: E
authors.lastname: Spillum
authors.affiliation: Philips BioCell, Gydevang 42, 3450, Allerød, Denmark.
authors.forename: S
authors.initials: S
authors.lastname: Kroghsbo
authors.affiliation: Department of Clinical Microbiology, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200, Aarhus, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, 9000, Aalborg, Denmark.
authors.forename: T E
authors.initials: TE
authors.lastname: Sondergaard
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D008853:Microscopy; D013997:Time Factors; D059008:Time-Lapse Imaging
chemical_list: D000900:Anti-Bacterial Agents