title: Hydrazine selective dual signaling chemodosimetric probe in physiological conditions and its application in live cells.
abstract: A rhodamine-cyanobenzene conjugate, (E)-4-((2-(3',6'-bis(diethylamino)-3-oxospiro[isoindoline-1,9'-xanthene]-2-yl)ethylimino)methyl)benzonitrile (1), which structure has been elucidated by single crystal X-ray diffraction, was synthesized for selective fluorescent "turn-on" and colorimetric recognition of hydrazine at physiological pH 7.4. It was established that 1 detects hydrazine up to 58 nM. The probe is useful for the detection of intracellular hydrazine in the human breast cancer cells MCF-7 using a fluorescence microscope. Spirolactam ring opening of 1, followed by its hydrolysis, was established as a probable mechanism for the selective sensing of hydrazine.
authors.affiliation: Department of Chemistry, The University of Burdwan, Burdwan, 713104 West Bengal, India.
authors.forename: Sandip
authors.initials: S
authors.lastname: Nandi
authors.affiliation: Department of Chemistry, The University of Burdwan, Burdwan, 713104 West Bengal, India.
authors.forename: Animesh
authors.initials: A
authors.lastname: Sahana
authors.affiliation: Department of Chemistry, The University of Burdwan, Burdwan, 713104 West Bengal, India.
authors.forename: Sandip
authors.initials: S
authors.lastname: Mandal
authors.affiliation: Department of Zoology, Visva Bharati University, Santiniketan, West Bengal, India.
authors.forename: Archya
authors.initials: A
authors.lastname: Sengupta
authors.affiliation: Department of Zoology, Visva Bharati University, Santiniketan, West Bengal, India.
authors.forename: Ansuman
authors.initials: A
authors.lastname: Chatterjee
authors.affiliation: Institute of Condensed Matter and Nanosciences, Molecules, Solids and Reactivity (IMCN/MOST), Université catholique de Louvain, Place L. Pasteur 1, 1348 Louvain-la-Neuve, Belgium. Electronic address: damir.a.safin@gmail.com.
authors.forename: Damir A
authors.initials: DA
authors.lastname: Safin
authors.affiliation: Institute of Condensed Matter and Nanosciences, Molecules, Solids and Reactivity (IMCN/MOST), Université catholique de Louvain, Place L. Pasteur 1, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Babashkina
authors.affiliation: Institute of Condensed Matter and Nanosciences, Molecules, Solids and Reactivity (IMCN/MOST), Université catholique de Louvain, Place L. Pasteur 1, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Nikolay A
authors.initials: NA
authors.lastname: Tumanov
authors.affiliation: Institute of Condensed Matter and Nanosciences, Molecules, Solids and Reactivity (IMCN/MOST), Université catholique de Louvain, Place L. Pasteur 1, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Yaroslav
authors.initials: Y
authors.lastname: Filinchuk
authors.affiliation: Department of Chemistry, The University of Burdwan, Burdwan, 713104 West Bengal, India. Electronic address: ddas100in@yahoo.com.
authors.forename: Debasis
authors.initials: D
authors.lastname: Das
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D006834:Hydrazines / Q000737:chemistry*; D061986:MCF-7 Cells; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D009570:Nitriles / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D011789:Quantum Theory; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014966:Xanthenes / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity
chemical_list: C000608971:4-(2-((3',6'-bis(diethylamino)-3-oxospiro(isoindoline-1,9'-xanthene)-2-yl)ethylimino)methyl)benzonitrile; D005456:Fluorescent Dyes; D006834:Hydrazines; D009570:Nitriles; D012235:Rhodamines; D014966:Xanthenes; C029424:hydrazine
keywords: Cell imaging; Chemodosimetric probe; Hydrazine; Rhodamine; “Turn-on” fluorescence