title: Prenatal exposure to fipronil disturbs maternal aggressive behavior in rats.
abstract: Fipronil is a second-generation phenilpirazol insecticide that is used in agriculture and veterinary medicine for protection against fleas, ticks, ants, cockroaches and other pests. The insecticide blocks the chloride channels associated with the gamma-amino butyric acid (GABA) receptors in mammals and the chloride channels associated with the GABA and glutamate (Glu) receptors in insects. In this study, a commercial product that contain fipronil was administered orally to pregnant Wistar rats at dosages of 0.1, 1.0, or 10.0 mg/kg/day from the 6th to the 20th day of gestation (n=10 pregnant rats/group) to assess the maternal aggressive behavior (on the 6th day of lactation) and the histopathology of the ovaries and the thyroid gland of the dams. The fipronil caused a disturbance of the maternal aggressive behavior; the aggression against a male intruder decreased at the lowest dose, but increased at the highest dose, without interfering with the general activity of the dams in the open field test at either dose. The histopathological analysis revealed no abnormalities. The differential effects of fipronil behavior appeared to be a consequence of actions on central nervous system areas that control these behaviors. We suggest that fipronil acts on maternal aggressive behavior through GABA(A) receptors.
authors.affiliation: Undergraduate in Biological Science, Universidade Presbiteriana Mackenzie, São Paulo, Brazil. Electronic address: juliazaccam@gmail.com.
authors.forename: Julia Z
authors.initials: JZ
authors.lastname: Magalhães
authors.affiliation: Graduate Program of Toxicology and Toxicological Analysis in School of Pharmaceutical Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Mariana S B
authors.initials: MS
authors.lastname: Udo
authors.affiliation: Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Angélica M
authors.initials: AM
authors.lastname: Sánchez-Sarmiento
authors.affiliation: Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Marcelo P N
authors.initials: MP
authors.lastname: Carvalho
authors.affiliation: Graduate Program of Environmental and Experimental Pathology and Graduate Program of Dentistry, Paulista University, São Paulo, Brazil.
authors.forename: Maria M
authors.initials: MM
authors.lastname: Bernardi
authors.affiliation: Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Helenice S
authors.initials: HS
authors.lastname: Spinosa
mesh_terms: D000284:Administration, Oral; D000374:Aggression / Q000187:drug effects*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D008425:Maternal Behavior / Q000187:drug effects*; D009043:Motor Activity / Q000187:drug effects; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000523:psychology*; D011720:Pyrazoles / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007306:Insecticides; D011720:Pyrazoles; C082360:fipronil
keywords: Fipronil; Maternal aggressive behavior; Toxicity