title: Seasonal effect on biomarkers of exposure to petroleum hydrocarbons in fish from Kuwait's marine area.
abstract: The aquatic biota of the Arabian Gulf deals with exposure to chronic oil pollution, several constituents of which cause induction of Cytochrome P450 1A that serves as a biomarker of AhR ligand exposure. In this study, fluorescent aromatic compounds (FACs) in bile and 7-ethoxyresorufin-o-deethylase (EROD) catalytic activity were determined as a measure of exposure biomarkers in two fish species, yellow fin seabream (Acanthopagrus latus) and tonguesole (Cynoglossus arel) captured from Kuwait Bay and outside the Bay area. FACs in fish bile determined by using fixed-wavelength fluorescence (FF) showed high fluorescence ratios between FF290/335 and FF380/430 indicating predominant exposure to low molecular weight, naphthalene-rich petroleum products (375±91.0 pg ml(-1)). Exposures to benzo(a)pyrene-type high-molecular weight polycyclic aromatic hydrocarbons (PAHs) originating from burnt fuel were also present in appreciable concentration in the bile. The ratio of petrogenic to pyrogenic hydrocarbon was twofold higher in winter compared to summer months in both species. Seasonal effect on EROD was significant in tonguesole in Auha site (P<0.05); whereas seabream resisted seasonal change. Tonguesole is considered to be a suitable bioindicator of oil pollution in Kuwait Bay area.
authors.affiliation: Environmental Pollution and Climate Program, Environment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, P.O. Box 24885, Safat, 13109, Kuwait. Electronic address: mbeg@kisr.edu.kw.
authors.forename: M U
authors.initials: MU
authors.lastname: Beg
authors.affiliation: Environmental Pollution and Climate Program, Environment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, P.O. Box 24885, Safat, 13109, Kuwait.
authors.forename: S N
authors.initials: SN
authors.lastname: Al-Subiai
authors.affiliation: Environmental Pollution and Climate Program, Environment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, P.O. Box 24885, Safat, 13109, Kuwait.
authors.forename: N
authors.initials: N
authors.lastname: Al-Jandal
authors.affiliation: Environmental Pollution and Climate Program, Environment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, P.O. Box 24885, Safat, 13109, Kuwait.
authors.forename: S A
authors.initials: SA
authors.lastname: Butt
authors.affiliation: Environmental Pollution and Climate Program, Environment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, P.O. Box 24885, Safat, 13109, Kuwait.
authors.forename: K R
authors.initials: KR
authors.lastname: Beg
authors.affiliation: Environmental Pollution and Climate Program, Environment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, P.O. Box 24885, Safat, 13109, Kuwait.
authors.forename: M
authors.initials: M
authors.lastname: Al-Husaini
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D054750:Ecotoxicology / Q000379:methods*; D004781:Environmental Exposure / Q000009:adverse effects / Q000032:analysis; D005412:Flatfishes / Q000378:metabolism*; D005453:Fluorescence; D007730:Kuwait; D010578:Petroleum / Q000009:adverse effects*; D059392:Petroleum Pollution / Q000009:adverse effects; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects / Q000737:chemistry; D021541:Sea Bream / Q000378:metabolism*; D012621:Seasons; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
chemical_list: D015415:Biomarkers; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System; D019363:Cytochrome P-450 CYP1A1
keywords: Cytochrome P450 1A; EROD; FACs; Kuwait Bay; Seabream; Tonguesole