title: Fluid and Resistive Tethered Lipid Membranes on Nanoporous Substrates.
abstract: Cell membranes perform important biological roles including compartmentalization, signaling, and transport of nutrients. Supported lipid membranes mimic the behavior of cell membranes and are an important model tool for studying membrane properties in a controlled laboratory environment. Lipid membranes may be supported on solid substrates; however, protein and lipid interactions with the substrate typically result in their denaturation. In this report, we demonstrate the formation of intact lipid membranes tethered on nanoporous metal thin films obtained via a dealloying process. Uniform lipid membranes were formed when the surface defect density of the nanoporous metal film was significantly reduced through a two-step dealloying process reported here. We show that the tethered lipid membranes on nanoporous metal substrates maintain both fluidity and electrical resistivity, which are key attributes to naturally occurring lipid membranes. The lipid assemblies supported on nanoporous metals provide a new platform for investigating lipid membrane properties, and potentially membrane proteins, for numerous applications including next generation biosensor platforms, targeted drug-delivery, and energy harvesting devices.
authors.forename: Gautam
authors.initials: G
authors.lastname: Gupta
authors.forename: Kyle
authors.initials: K
authors.lastname: Staggs
authors.forename: Aditya D
authors.initials: AD
authors.lastname: Mohite
authors.forename: Jon K
authors.initials: JK
authors.lastname: Baldwin
authors.forename: Srinivas
authors.initials: S
authors.lastname: Iyer
authors.forename: Rangachary
authors.initials: R
authors.lastname: Mukundan
authors.forename: Amit
authors.initials: A
authors.lastname: Misra
authors.affiliation: School of Mechanical Engineering, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.
authors.forename: Antonia
authors.initials: A
authors.lastname: Antoniou
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Dattelbaum
mesh_terms: D008563:Membrane Lipids / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D058608:Nanopores
chemical_list: D008563:Membrane Lipids