title: Six- and seven-dimensional experiments by combination of sparse random sampling and projection spectroscopy dedicated for backbone resonance assignment of intrinsically disordered proteins.
abstract: Two novel six- and seven-dimensional NMR experiments are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in four indirectly detected dimensions and synchronous sampling in the additional dimensions using projection spectroscopy principle. The resulted data sets could be processed as five-dimensional data using existing software. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel experiments were successfully tested using 1 mM sample of α-synuclein on 600 and 800 MHz NMR spectrometers equipped with standard room temperature probes. The experiments allowed backbone assignment from a 1-day acquisition.
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02089, Warsaw, Poland.
authors.forename: Szymon
authors.initials: S
authors.lastname: Żerko
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02089, Warsaw, Poland. kozmin@chem.uw.edu.pl.
authors.forename: Wiktor
authors.initials: W
authors.lastname: Koźmiński
mesh_terms: D064267:Intrinsically Disordered Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods
chemical_list: D064267:Intrinsically Disordered Proteins
keywords: High-dimensionality NMR; Intrinsically disordered proteins; Non-uniform sampling; Projection spectroscopy; Resonance assignment