title: Protective Effects of MDG-1, a Polysaccharide from Ophiopogon japonicus on Diabetic Nephropathy in Diabetic KKAy Mice.
abstract: Ophiopogon japonicus is a traditional Chinese medicine that might be effective for treating type 2 diabetes. Recent research confirmed that MDG-1, a polysaccharide from O. japonicas, activates the PI3K/Akt signaling pathway and improves insulin sensitivity in a diabetic KKAy mouse model, but little is known about its effects on diabetic nephropathy. In this study, KKAy mice were orally administered distilled water (control group), MDG-1, or rosiglitazone for 12 weeks. Blood glucose levels were tested every two weeks for the fed mice. At 6 and 12 weeks, blood samples were collected for biochemical examination. At the end of the experiment, all kidney tissues were collected for histological examination and western blot analysis. Results show that MDG-1 (300 mg/kg) significantly decreased the levels of blood glucose, triglycerides, blood urine nitrogen and albumin, and significantly inhibited the expression of transforming growth factor-beta 1 and connective tissue growth factor. Moreover, MDG-1 could alleviate glomerular mesangial expansion and tubulointerstitial fibrosis in the diabetic mice, as confirmed by histopathological examination. These data indicated that MDG-1 ameliorates renal disease in diabetic mice by reducing hyperglycemia, hyperinsulinemia, and hyperlipidemia, and by inhibiting intracellular signaling pathways.
authors.affiliation: Engineering Research Center of Modern Preparation Technology of TCM, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. amoness@163.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Engineering Research Center of Modern Preparation Technology of TCM, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. newhelenhappy@163.com.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Shi
authors.affiliation: Engineering Research Center of Modern Preparation Technology of TCM, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. shilinmochen@126.com.
authors.forename: Ling-Yi
authors.initials: LY
authors.lastname: Wang
authors.affiliation: Murad Research Institute for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. jinwen.xu88@gmail.com.
authors.forename: Jin-Wen
authors.initials: JW
authors.lastname: Xu
authors.affiliation: Engineering Research Center of Modern Preparation Technology of TCM, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. fyi_shutcm@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D055513:Connective Tissue Growth Factor / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D003928:Diabetic Nephropathies / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004334:Drug Administration Schedule; D005786:Gene Expression Regulation / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D011134:Polysaccharides / Q000008:administration & dosage* / Q000494:pharmacology; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000008:administration & dosage / Q000627:therapeutic use; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D001786:Blood Glucose; C526318:CCN2 protein, mouse; D007004:Hypoglycemic Agents; D011134:Polysaccharides; C508961:Tgfb1 protein, mouse; D045162:Thiazolidinediones; D053773:Transforming Growth Factor beta1; D014280:Triglycerides; C517472:polysaccharide MDG-1; D000077154:Rosiglitazone; D055513:Connective Tissue Growth Factor
keywords: Ophiopogon japonicus; diabetic nephropathy; polysaccharide