title: In vitro migration and proliferation ("wound healing") potential of mesenchymal stromal cells generated from human CD271(+) bone marrow mononuclear cells.
abstract: BACKGROUND
  Emerging evidence indicates that mesenchymal stromal cells (MSCs) isolated from different tissue sources may be used in vivo as tissue restorative agents. To date, there is no evidence, however, on migration and proliferation ("wound healing") potential of different subsets of MSCs. The main goal of this study was therefore to compare the in vitro "wound healing" capacity of MSCs generated from positively selected CD271(+) bone marrow mononuclear cells (CD271-MSCs) and MSCs generated by plastic adherence (PA-MSCs).
  
  
  METHODS
  The in vitro model of wound healing (CytoSelect™ 24-Well Wound Healing Assay) was used in order to compare the migration and proliferation potential of CD271-MSCs and PA-MSCs of passage 2 and 4 cultured in presence or absence of growth factors or cytokines.
  
  
  RESULTS
  CD271-MSCs of both passages when compared to PA-MSCs demonstrated a significantly higher potential to close the wound 12 and 24 h after initiation of the wound healing assay (P < 0.003 and P < 0.002, respectively). Noteworthy, the migration capacity of PA-MSCs of second passage was significantly improved after stimulation with FGF-2 (P < 0.02), PDGF-BB (P < 0.006), MCP-1 (P < 0.002) and IL-6 (P < 0.03), whereas only TGF-β enhanced significantly migration process of PA-MSCs of P4 12 h after the treatment (P < 0.02). Interestingly, treatment of CD271-MSCs of both passages with growth factors or cytokines did not affect their migratory potential.
  
  
  CONCLUSIONS
  Our in vitro data provide the first evidence that CD271-MSCs are significantly more potent in "wound healing" than their counterparts PA-MSCs.
authors.affiliation: Department for Children and Adolescents, Division for Stem Cell Transplantation and Immunology, Goethe University Hospital, Frankfurt am Main, Germany. hatixhe.pupovci@uni-pr.edu.
authors.forename: Hatixhe
authors.initials: H
authors.lastname: Latifi-Pupovci
authors.affiliation: Department for Children and Adolescents, Division for Stem Cell Transplantation and Immunology, Goethe University Hospital, Frankfurt am Main, Germany. zyrafete.kuci@kgu.de.
authors.forename: Zyrafete
authors.initials: Z
authors.lastname: Kuçi
authors.affiliation: Department for Children and Adolescents, Division for Stem Cell Transplantation and Immunology, Goethe University Hospital, Frankfurt am Main, Germany. sibylle.wehner@kgu.de.
authors.forename: Sibylle
authors.initials: S
authors.lastname: Wehner
authors.affiliation: Institute of Transfusion Medicine and German Red Cross Blood Center Frankfurt, Frankfurt am Main, Germany. H.Boenig@blutspende.de.
authors.forename: Halvard
authors.initials: H
authors.lastname: Bönig
authors.affiliation: Institute of Pathology, Goethe University Hospital, Frankfurt am Main, Germany. Ralf.Lieberz@kgu.de.
authors.forename: Ralf
authors.initials: R
authors.lastname: Lieberz
authors.affiliation: Department for Children and Adolescents, Division for Stem Cell Transplantation and Immunology, Goethe University Hospital, Frankfurt am Main, Germany. thomas.klingebiel@kgu.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Klingebiel
authors.affiliation: Department for Children and Adolescents, Division for Stem Cell Transplantation and Immunology, Goethe University Hospital, Frankfurt am Main, Germany. peter.bader@kgu.de.
authors.forename: Peter
authors.initials: P
authors.lastname: Bader
authors.affiliation: Department for Children and Adolescents, Division for Stem Cell Transplantation and Immunology, Goethe University Hospital, Frankfurt am Main, Germany. selim.kuci@kgu.de.
authors.forename: Selim
authors.initials: S
authors.lastname: Kuçi
mesh_terms: D000293:Adolescent; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010641:Phenotype; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D055815:Young Adult
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; C484065:NGFR protein, human; D009419:Nerve Tissue Proteins; D017475:Receptors, Nerve Growth Factor