title: The Creation of a Model for Ex Vivo Development of Postoperative Adhesions.
abstract: BACKGROUND
  Postoperative adhesions occur in the overwhelming majority of patients after laparotomy and laparoscopy. The clinical consequences of adhesions can be devastating. Adhesions are believed to contribute to infertility and are the most common cause of small bowel obstruction and abdominal pelvic pain. Our objective is to develop a model for ex vivo development of postoperative adhesions.
  
  
  METHODS
  Peritoneal tissue strips were obtained from Sprague Dawley rats and were abraded by scraping the tissue surface with a scalpel. Tissues were incubated in a special medium, and visible adhesion bands were assessed. Experiments were performed in a university setting.
  
  
  RESULTS
  Adhesion bands were observed as early as 24 hours after abrasion. No adhesion bands were observed in the control tissue strips.
  
  
  CONCLUSIONS
  This is a model system for the creation of actual adhesive bands utilizing an ex vivo culture of peritoneal strips.
authors.affiliation: Department of Obstetrics and Gynecology, The C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, MI, USA gsaed@med.wayne.edu.
authors.forename: Ghassan M
authors.initials: GM
authors.lastname: Saed
authors.affiliation: Department of Obstetrics and Gynecology, The C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, MI, USA.
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Fletcher
authors.affiliation: Department of Obstetrics and Gynecology, Clinical and Translational Sciences, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Diamond
mesh_terms: D000818:Animals; D005260:Female; D023421:Models, Animal; D009924:Organ Culture Techniques / Q000379:methods; D010537:Peritoneum / Q000473:pathology* / Q000601:surgery; D011183:Postoperative Complications / Q000209:etiology / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000267:Tissue Adhesions / Q000209:etiology / Q000473:pathology*
keywords: abrasion; adhesions; ex vivo model; peritoneum