title: Biochanin A protects dopaminergic neurons against lipopolysaccharide-induced damage and oxidative stress in a rat model of Parkinson's disease.
abstract: Parkinson's disease (PD) is the second most common neurodegenerative disease, which is characterized by loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc). Accumulated evidences have suggested that oxidative stress is closely associated with the dopaminergic neurodegeneration of PD that can be protected by antioxidants. Biochanin A that is an O-methylated isoflavone in chickpea is investigated to explore its protective mechanism on dopaminergic neurons of the unilateral lipopolysaccharide (LPS)-injected rat. The results showed that biochanin A significantly improved the animal model's behavioral symptoms, prevented the loss of dopaminergic neurons and inhibited the deleterious microglia activation in the LPS-induced rats. Moreover, biochanin A inhibited nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase) activation and malondialdehyde (MDA) production, increased superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in the rat brain. These results suggested that biochanin A might be a natural candidate with protective properties on dopaminergic neurons against the PD.
authors.affiliation: Department of Pharmacology, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, PR China; School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, PR China; School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
authors.forename: Can
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Pharmacology, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, PR China.
authors.forename: Wang-Yang
authors.initials: WY
authors.lastname: Wu
authors.affiliation: Department of Food, Nutrition, and Packaging Sciences, Clemson University, Clemson, SC 29634, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, PR China.
authors.forename: Yang-Yang
authors.initials: YY
authors.lastname: Wu
authors.affiliation: Department of Pharmacology, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, PR China.
authors.forename: Wei-Zu
authors.initials: WZ
authors.lastname: Li
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China. Electronic address: hanqchen@hfut.edu.cn.
authors.forename: Han-Qing
authors.initials: HQ
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, PR China. Electronic address: yinyanyan5678@126.com.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Yin
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D059290:Dopaminergic Neurons / Q000187:drug effects*; D004789:Enzyme Activation / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D008262:Macrophage Activation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D017628:Microglia / Q000187:drug effects; D019255:NADPH Oxidases / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D010302:Parkinson Disease, Secondary / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000096:biosynthesis
chemical_list: D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D008315:Malondialdehyde; D019833:Genistein; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D019255:NADPH Oxidases; C004541:biochanin A
keywords: Biochanin A; Dopaminergic neuron; Microglia; NADPH oxidase; Oxidative stress; Parkinson's disease