title: Production of a bacteriocin-like inhibitory substance by Leuconostoc mesenteroides subsp. dextranicum 213M0 isolated from Mongolian fermented mare milk, airag.
abstract: Strain 213M0 was selected with productivity of a bacteriocin-like inhibitory substance (BLIS) among 235 strains of lactic acid bacteria (LAB) isolated from Mongolian fermented milk 'airag'. Strain 213M0 was species-identified as Leuconostoc mesenteroides subsp. dextranicum by morphological observation, carbohydrate fermentation profiling and sequencing the 16S rRNA gene. Incubation temperature proper to produce the BLIS was 25°C rather than 30 and 37°C, and the production actively proceeded during the exponential growth phase of the producer cells. Antibacterial effect of BLIS 213M0 was limited to all nine strains of Listeria sp. bacteria and seven strains of LAB cocci among 53 tested strains, which corresponds to a typical feature of the class IIa pediocin-like bacteriocins. BLIS 213M0 was not inactivated in every broad pH range solution (pH 2.0-11.0), and was stable against storage at 25°C for 1 week and heating at 121°C for 15 min under pH 4.5. Peptide frame of BLIS 213M0 was confirmed by inactivation with some peptidases, and then its molecular weight was estimated to be 2.6-3.0 kDa using an in situ activity assay following sodium dodecyl sulfate polyacrylamide gel electrophoresis. The estimated size was different from the other Leuconostoc bacteriocins already reported. These results suggest that BLIS 213M0 would be a novel listericidal bacteriocin.
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Arakawa
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: Saki
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Aikawa
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Hano
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: Tsognemekh
authors.initials: T
authors.lastname: Bolormaa
authors.affiliation: School of Veterinary Science and Biotechnology, Mongolian State University of Agriculture, Ulaanbaatar, Mongolia.
authors.forename: Sedkhuu
authors.initials: S
authors.lastname: Burenjargal
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.|Faculty of Food Culture, Kurashiki Sakuyo University, Kurashiki, Okayama, Japan.
authors.forename: Taku
authors.initials: T
authors.lastname: Miyamoto
mesh_terms: D000818:Animals; D001430:Bacteriocins / Q000096:biosynthesis* / Q000145:classification / Q000494:pharmacology*; D043302:Cultured Milk Products / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D006736:Horses; D006863:Hydrogen-Ion Concentration; D000070007:Leuconostoc mesenteroides / Q000302:isolation & purification* / Q000378:metabolism*; D008087:Listeria / Q000187:drug effects; D008970:Molecular Weight
chemical_list: D001430:Bacteriocins
keywords: airag; bacteriocin; lactic acid bacteria; leuconostoc mesenteroides; listericidal activity