title: Pseudomonas aeruginosa flagellin as an adjuvant: superiority of a conjugated form of flagellin versus a mixture with a human immunodeficiency virus type 1 vaccine candidate in the induction of immune responses.
abstract: In the present study, the adjuvant activity of flagellin was compared, in the conjugated and mixed forms, against a peptide vaccine from human immunodeficiency virus type 1 (HIV-1) p24-Nef vaccine candidate. Mice were immunized with the HIV-1 p24-Nef peptide with flagellin in both conjugated and mixed forms. Lymphocyte proliferation, CTL activity, and IL-4 and IFN-γ cytokines were evaluated by bromodeoxyuridine, carboxyfluorescein succinimidyl ester and ELISA methods, respectively. At the same time, the frequency of IFN-γ-producing T-lymphocytes, as well as total and isotype-specific antibodies, were assessed by ELISPOT and indirect ELISA, respectively. Our experimental results showed that the immunized mice with the HIV-1 p24-Nef conjugated or mixed forms of flagellin led to increases in the proliferative responses and Th1 cytokine pattern. The conjugated form of vaccine led to increased CTL activity and a Th1 cytokine pattern of immune responses, as well as an IgM isotype of humoral responses in comparison with the mixed form. The flagellin-conjugated vaccine seems to be more potent in increasing vaccine immunogenicity.
authors.affiliation: Department of Biology, Faculty of Sciences, Qom branch of Islamic Azad University, Qom, Iran.
authors.forename: Safura
authors.initials: S
authors.lastname: Delavari
authors.affiliation: Department of Biology, Faculty of Sciences, Qom branch of Islamic Azad University, Qom, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Sohrabi
authors.affiliation: Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mehdi Shafiee
authors.initials: MS
authors.lastname: Ardestani
authors.affiliation: Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Sobhan
authors.initials: S
authors.lastname: Faezi
authors.affiliation: Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Tebianian
authors.affiliation: Department of Medical Virology, Razi Vaccine and Serum Research Institute, Karaj, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Taghizadeh
authors.affiliation: Molecular Pathology Laboratory, Cancer Molecular Pathology Research Center, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Arezoo
authors.initials: A
authors.lastname: Shajiei
authors.affiliation: Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Seyed Younes
authors.initials: SY
authors.lastname: Hosseini
authors.affiliation: Department of Medical Virology, Razi Vaccine and Serum Research Institute, Karaj, Iran.|Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Moghaddampour
authors.affiliation: Department of Immunology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Mahdavi
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000235:genetics; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D005260:Female; D005408:Flagellin / Q000235:genetics / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D005408:Flagellin; D015847:Interleukin-4; D007371:Interferon-gamma