title: Mature Erythrocyte Surface Antigen Protein Identified in the Serum of Plasmodium falciparum-Infected Patients.
abstract: This study was performed to identify circulating Plasmodium falciparum proteins in patient serum, which may be useful as diagnostic markers. Depletion of highly abundant proteins from each pooled serum sample obtained from P. falciparum-infected patients and healthy individuals was performed using the Proteoseek Antibody-Based Albumin/IgG Removal Kit (Thermo Scientific, Rockford, IL). In analysis 1, the depleted serum was analyzed directly by NanoLC-MS/MS. In analysis 2, the depleted serum was separated by two-dimensional electrophoresis followed by western blot analysis. Subsequently, the selected band was analyzed by NanoLC-MS/MS. The result of analysis 1 revealed the presence of two mature erythrocyte surface antigen (MESA) proteins and chloroquine resistance transporter protein (PfCRT). In addition, analysis 2 revealed an antigenic 75-kDa band when the membrane was probed with purified IgG from the pooled serum obtained from P. falciparum-infected patients. MS/MS analysis of this protein band revealed fragments of P. falciparum MESA proteins. Thus, in this study, two different analyses revealed the presence of Plasmodium MESA protein in pooled serum from malaria patients; thus, this protein should be further investigated to determine its usefulness as a diagnostic marker.
authors.affiliation: Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang, Malaysia; Kuching Health Office, Ministry of Health, Kuching, Sarawak, Malaysia; Malaysia Genome Institute, Jalan Bangi, 43000 Kajang, Selangor, Malaysia.
authors.forename: Nurul Shazalina
authors.initials: NS
authors.lastname: Zainudin
authors.affiliation: Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang, Malaysia; Kuching Health Office, Ministry of Health, Kuching, Sarawak, Malaysia; Malaysia Genome Institute, Jalan Bangi, 43000 Kajang, Selangor, Malaysia.
authors.forename: Nurulhasanah
authors.initials: N
authors.lastname: Othman
authors.affiliation: Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang, Malaysia; Kuching Health Office, Ministry of Health, Kuching, Sarawak, Malaysia; Malaysia Genome Institute, Jalan Bangi, 43000 Kajang, Selangor, Malaysia.
authors.forename: Jamail
authors.initials: J
authors.lastname: Muhi
authors.affiliation: Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang, Malaysia; Kuching Health Office, Ministry of Health, Kuching, Sarawak, Malaysia; Malaysia Genome Institute, Jalan Bangi, 43000 Kajang, Selangor, Malaysia.
authors.forename: Asmahani Azira
authors.initials: AA
authors.lastname: Abdu Sani
authors.affiliation: Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang, Malaysia; Kuching Health Office, Ministry of Health, Kuching, Sarawak, Malaysia; Malaysia Genome Institute, Jalan Bangi, 43000 Kajang, Selangor, Malaysia rahmah8485@gmail.com.
authors.forename: Rahmah
authors.initials: R
authors.lastname: Noordin
mesh_terms: D000293:Adolescent; D000328:Adult; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D015153:Blotting, Western; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D016778:Malaria, Falciparum / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D008296:Malaysia; D008875:Middle Aged; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification; D012709:Serum Albumin / Q000032:analysis; D055815:Young Adult
chemical_list: D000953:Antigens, Protozoan; D000954:Antigens, Surface; D007074:Immunoglobulin G; C079081:MESA protein, Plasmodium falciparum; D015800:Protozoan Proteins; D012709:Serum Albumin