title: Application of a Cu-chitosan/multiwalled carbon nanotube film-modified electrode for the sensitive determination of rutin.
abstract: A new sensitive electrochemical sensor, a glassy carbon electrode modified with chemically cross-linked copper-complexed chitosan/multiwalled carbon nanotubes (Cu-CS/MWCNT/GCE), for rutin analysis was constructed. Experimental investigations of the influence of several parameters showed that the rutin can effectively accumulate on the surface of the Cu-CS/MWCNT/GCE, which accumulation caused a pair of well-defined redox peaks in the electrochemical signal when measurements were carried out in Britton-Robinson buffer solution (pH 3, 0.04 M). The surface of the Cu-CS/MWCNT/GCE was characterized by field-emission scanning electron microscopy, transmission electron microscopy, and X-ray diffractometry analysis. In a rutin concentration range of 0.05-100 μM and under optimized conditions, a linear relationship between the oxidation peak current of rutin and its concentration was obtained with a detection limit of 0.01 μM. The Cu-CS/MWCNT/GCE showed good selectivity, stability, and reproducibility. Moreover, the sensor was used to determine the presence of rutin in fruits with satisfactory results.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Razi University, 67155 Kermanshah, Iran. Electronic address: mbgholivand2013@gmail.com.
authors.forename: Mohammad Bagher
authors.initials: MB
authors.lastname: Gholivand
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Razi University, 67155 Kermanshah, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Mohammadi-Behzad
authors.affiliation: Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan.
authors.forename: Hossein
authors.initials: H
authors.lastname: Hosseinkhani
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D002957:Citrus / Q000737:chemistry; D032082:Citrus aurantiifolia / Q000737:chemistry; D032084:Citrus sinensis / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D005638:Fruit / Q000737:chemistry; D057230:Limit of Detection; D027845:Malus / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012431:Rutin / Q000032:analysis*
chemical_list: D037742:Nanotubes, Carbon; D012431:Rutin; D048271:Chitosan
keywords: Chitosan; Modified glassy carbon electrode; Multiwalled carbon nanotubes; Rutin