title: Global Identification of Genes Specific for Rice Meiosis.
abstract: The leptotene-zygotene transition is a major step in meiotic progression during which pairing between homologous chromosomes is initiated and double strand breaks occur. OsAM1, a homologue of maize AM1 and Arabidopsis SWI1, encodes a protein with a coiled-coil domain in its central region that is required for the leptotene-zygotene transition during rice meiosis. To gain more insight into the role of OsAM1 in rice meiosis and identify additional meiosis-specific genes, we characterized the transcriptomes of young panicles of Osam1 mutant and wild-type rice plants using RNA-Seq combined with bioinformatic and statistical analyses. As a result, a total of 25,750 and 28,455 genes were expressed in young panicles of wild-type and Osam1 mutant plants, respectively, and 4,400 differentially expressed genes (DEGs; log2 Ratio ≥ 1, FDR ≤ 0.05) were identified. Of these DEGs, four known rice meiosis-specific genes were detected, and 22 new putative meiosis-related genes were found by mapping these DEGs to reference biological pathways in the KEGG database. We identified eight additional well-conserved OsAM1-responsive rice meiotic genes by comparing our RNA-Seq data with known meiotic genes in Arabidopsis and fission yeast.
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education/ Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modem Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Bingwei
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education/ Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modem Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Xu
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education/ Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modem Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Shiquan
authors.initials: S
authors.lastname: Bian
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education/ Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modem Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education/ Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modem Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Minghong
authors.initials: M
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Zhukuan
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education/ Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modem Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Hengxiu
authors.initials: H
authors.lastname: Yu
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D019295:Computational Biology; D015723:Gene Library; D017343:Genes, Plant; D008540:Meiosis / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D010940:Plant Proteins