title: Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering.
abstract: We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers. The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes). In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix. However, the use of chitosan as an additive stimulated proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds. The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment. Polymer scaffolds (without cells) accelerated complete healing of skin wounds in vivo irrespective of their composition healing, pure chitosan sponge being most effective. We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds.
authors.affiliation: National Research Centre Kurchatov Institute, Moscow, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Romanova
authors.affiliation: National Research Centre Kurchatov Institute, Moscow, Russia.
authors.forename: T E
authors.initials: TE
authors.lastname: Grigor'ev
authors.affiliation: National Research Centre Kurchatov Institute, Moscow, Russia.
authors.forename: M E
authors.initials: ME
authors.lastname: Goncharov
authors.affiliation: MiraksBioFarma, Khimki, Moscow Region, Russia.
authors.forename: S G
authors.initials: SG
authors.lastname: Rudyak
authors.affiliation: National Research Centre Kurchatov Institute, Moscow, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Solov'yova
authors.affiliation: National Research Centre Kurchatov Institute, Moscow, Russia.
authors.forename: S T
authors.initials: ST
authors.lastname: Krasheninnikov
authors.affiliation: National Research Centre Kurchatov Institute, Moscow, Russia.
authors.forename: V P
authors.initials: VP
authors.lastname: Saprykin
authors.affiliation: National Research Centre Kurchatov Institute, Moscow, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Sytina
authors.affiliation: National Research Centre Kurchatov Institute, Moscow, Russia.
authors.forename: S N
authors.initials: SN
authors.lastname: Chvalun
authors.affiliation: National Research Centre Kurchatov Institute, Moscow, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Pal'tsev
authors.affiliation: National Research Centre Kurchatov Institute, Moscow, Russia. a.a.pantel@gmail.com.
authors.forename: A A
authors.initials: AA
authors.lastname: Panteleev
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry; D055119:Elastic Modulus; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D015603:Keratinocytes / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing
chemical_list: D003470:Culture Media; D048271:Chitosan
keywords: biocompatibility; fibroblasts; keratinocytes; skin equivalent