title: Non-covalent Nanocomplexes of Folic Acid and Reducible Polyethylenimine for Survivin siRNA Delivery.
abstract: OBJECTIVE
  Efficient delivery of siRNA is critical for its therapeutic applications. This study was aimed at the design, synthesis and evaluation of a novel delivery system based on non-covalent complexes of folic acid (FA) and a reducible polyethylenimine (PEI) derivative, PEI-SS.
  
  
  METHODS
  PEI-SS was synthesized by crosslinking low-molecular weight PEI using a reducible crosslinking agent. PEI-SS-siRNA complexes were synthesized and further combined with FA to form FA/PEI-SS-siRNA nanocomplexes. These were then evaluated in two tumor cell lines for survivin siRNA delivery.
  
  
  RESULTS
  FA/PEI-SS-siRNA complexes were taken-up by both HeLa and A549 cells efficiently. This was not affected by the expression level of the folate receptor on the tumor cell surface. Furthermore, FA/PEI-SS-siRNA complexes reduced the level of survivin expression in both cell lines.
  
  
  CONCLUSIONS
  FA/PEI-SS is a potent siRNA carrier that warrants further evaluation.
authors.affiliation: College of Life Science, Jilin University, Changchun, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zheng
authors.affiliation: College of Life Science, Jilin University, Changchun, P.R. China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yang
authors.affiliation: College of Life Science, Jilin University, Changchun, P.R. China.
authors.forename: Mengqiao
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Life Science, Jilin University, Changchun, P.R. China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Life Science, Jilin University, Changchun, P.R. China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Teng
authors.affiliation: College of Life Science, Jilin University, Changchun, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
authors.affiliation: College of Life Science, Jilin University, Changchun, P.R. China lee.1339@osu.edu tenglesheng@jlu.edu.cn.
authors.forename: Lesheng
authors.initials: L
authors.lastname: Teng
authors.affiliation: College of Life Science, Jilin University, Changchun, P.R. China College of Pharmacy, The Ohio State University, Columbus, OH, U.S.A. lee.1339@osu.edu tenglesheng@jlu.edu.cn.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Lee
mesh_terms: D045744:Cell Line, Tumor; D058973:Folate Receptors, GPI-Anchored / Q000378:metabolism; D005492:Folic Acid / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D058726:Nanoconjugates / Q000737:chemistry*; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000494:pharmacology*; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D058973:Folate Receptors, GPI-Anchored; D051034:Inhibitor of Apoptosis Proteins; D058726:Nanoconjugates; D034741:RNA, Small Interfering; D000077022:Survivin; D011094:Polyethyleneimine; D005492:Folic Acid
keywords: Polyethylenimine; cancer; drug delivery; folate receptor; nanoparticle; siRNA