title: The non-competitive blockade of GABAA receptors by an aqueous extract of water hemlock (Cicuta douglasii) tubers.
abstract: Water hemlocks (Cicuta spp.) are acutely toxic members of the Umbellierae family; the toxicity is due to the presence of C17-polyacetylenes such as cicutoxin. There is only limited evidence of noncompetitive antagonism by C17-polyacetylenes at GABAA receptors. In this work with WSS-1 cells, we documented the noncompetitive blockade of GABAA receptors by an aqueous extract of water hemlock (Cicuta douglasii) and modulated the actions of the extract with a pretreatment of 10 μM midazolam.
authors.affiliation: Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Logan, UT, USA. Electronic address: Ben.Green@ars.usda.gov.
authors.forename: Benedict T
authors.initials: BT
authors.lastname: Green
authors.affiliation: Graduate Program in Animal Science, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Goulart
authors.affiliation: Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Logan, UT, USA.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Welch
authors.affiliation: Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Logan, UT, USA.
authors.forename: James A
authors.initials: JA
authors.lastname: Pfister
authors.affiliation: Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Logan, UT, USA.
authors.forename: Isabelle
authors.initials: I
authors.lastname: McCollum
authors.affiliation: Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Logan, UT, USA.
authors.forename: Dale R
authors.initials: DR
authors.lastname: Gardner
mesh_terms: D029981:Cicuta / Q000737:chemistry*; D058787:GABA-A Receptor Antagonists / Q000737:chemistry / Q000633:toxicity*; D057809:HEK293 Cells; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity*; D011963:Receptors, GABA-A / Q000737:chemistry
chemical_list: D058787:GABA-A Receptor Antagonists; D010936:Plant Extracts; D011963:Receptors, GABA-A
keywords: Barbiturates; Benzodiazepines; C(17)-polyacetylenes; Cicutoxin; Midazolam; Water hemlock