title: Effect of Saliva on the Tensile Bond Strength of Different Generation Adhesive Systems: An In-Vitro Study.
abstract: BACKGROUND
  Newer development of bonding agents have gained a better understanding of factors affecting adhesion of interface between composite and dentin surface to improve longevity of restorations.
  
  
  OBJECTIVE
  The present study evaluated the influence of salivary contamination on the tensile bond strength of different generation adhesive systems (two-step etch-and-rinse, two-step self-etch and one-step self-etch) during different bonding stages to dentin where isolation is not maintained.
  
  
  METHODS
  Superficial dentin surfaces of 90 extracted human molars were randomly divided into three study Groups (Group A: Two-step etch-and-rinse adhesive system; Group B: Two-step self-etch adhesive system and Group C: One-step self-etch adhesive system) according to the different generation of adhesives used. According to treatment conditions in different bonding steps, each Group was further divided into three Subgroups containing ten teeth in each. After adhesive application, resin composite blocks were built on dentin and light cured subsequently. The teeth were then stored in water for 24 hours before sending for testing of tensile bond strength by Universal Testing Machine. The collected data were then statistically analysed using one-way ANOVA and Tukey HSD test.
  
  
  RESULTS
  One-step self-etch adhesive system revealed maximum mean tensile bond strength followed in descending order by Two-step self-etch adhesive system and Two-step etch-and-rinse adhesive system both in uncontaminated and saliva contaminated conditions respectively.
  
  
  CONCLUSIONS
  Unlike One-step self-etch adhesive system, saliva contamination could reduce tensile bond strength of the two-step self-etch and two-step etch-and-rinse adhesive system. Furthermore, the step of bonding procedures and the type of adhesive seems to be effective on the bond strength of adhesives contaminated with saliva.
authors.affiliation: P.G Student, Department of Pedodontics & Preventive Dentistry, Sardar Patel Post Graduate Institute of Dental & Medical Sciences , Lucknow, India .
authors.forename: Nimisha
authors.initials: N
authors.lastname: Gupta
authors.affiliation: Professor & Head, Department Of Pedodontics & Preventive Dentistry, Sardar Patel Post Graduate Institute of Dental & Medical Sciences , Lucknow, India .
authors.forename: Abhay Mani
authors.initials: AM
authors.lastname: Tripathi
authors.affiliation: Reader, Department of Pedodontics & Preventive Dentistry, Sardar Patel Post Graduate Institute of Dental & Medical Sciences , Lucknow, India .
authors.forename: Sonali
authors.initials: S
authors.lastname: Saha
authors.affiliation: Senior Lecturer, Department of Pedodontics & Preventive Dentistry, Sardar Patel Post Graduate Institute of Dental & Medical Sciences , Lucknow, India .
authors.forename: Kavita
authors.initials: K
authors.lastname: Dhinsa
authors.affiliation: Senior Lecturer, Department of Pedodontics & Preventive Dentistry, Jaipur Dental College , Jaipur, India .
authors.forename: Aarti
authors.initials: A
authors.lastname: Garg
keywords: Adhesion; Aesthetic dentistry; Composite resin; Saliva