title: Silk-based stabilization of biomacromolecules.
abstract: Silk fibroin is a high molecular weight amphiphilic protein that self-assembles into robust biomaterials with remarkable properties including stabilization of biologicals and tunable release kinetics correlated to processing conditions. Cells, antibiotics,monoclonal antibodies and peptides, among other biologics, have been encapsulated in silk using various processing approaches and material formats. The mechanistic basis for the entrapment and stabilization features, along with insights into the modulation of release of the entrained compounds from silks will be reviewed with a focus on stabilization of bioactive molecules.
authors.affiliation: Department of Chemical and Biological Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA.
authors.forename: Adrian B
authors.initials: AB
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA.
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Kluge
authors.affiliation: Drug Product Technologies, Amgen, 1 Amgen Center Drive, Thousand Oaks, CA 91320, USA.
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Guziewicz
authors.affiliation: Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA.
authors.forename: Fiorenzo G
authors.initials: FG
authors.lastname: Omenetto
authors.affiliation: Department of Chemical and Biological Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA; Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA. Electronic address: david.kaplan@tufts.edu.
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
mesh_terms: D004355:Drug Stability; D046911:Macromolecular Substances / Q000737:chemistry; D047011:Silk / Q000737:chemistry*
chemical_list: D046911:Macromolecular Substances; D047011:Silk
keywords: Biologics; Interactions; Mechanisms; Processing; Recovery; Silk; Stabilization