title: Jinggangmycin increases fecundity of the brown planthopper, Nilaparvata lugens (Stål) via fatty acid synthase gene expression.
abstract: The antibiotic jinggangmycin (JGM) is mainly used in controlling the rice sheath blight, Rhizoctonia solani, in China. JGM also enhances reproduction of the brown planthopper (BPH), Nilaparvata lugens (Stål). To date, however, molecular mechanisms of the enhancement are unclear. Our related report documented the influence of foliar JGM sprays on ovarian protein content. Here, we used isobaric tags for relative and absolute quantitation (iTRAQ) protocols to analyze ovarian proteins of BPH females following JGM spray (JGM-S) and topical application (JGM-T). We recorded changes in expression of 284 proteins (142↑ and 142↓) in JGM-S compared to the JGM-S control group (S-control); 267 proteins were differentially expressed (130↑ and 137↓) in JGM-T compared to the JGM-T control group (T-control), of which, 22 proteins were up-regulated in both groups. Comparing the JGM-S to the JGM-T group, 114 proteins were differentially expressed (62↑ and 52↓). Based on the biological significance of fatty acids, pathway annotation and enrichment analysis, we designed a dsRNA construct to silence a gene encoding fatty acid synthase (FAS). FAS was more highly expressed in JGM-S vs S-control and JGM-S vs JGM-T groups. The dsFAS treatment reduced fecundity by about 46% and reduced ovarian and fat body fatty acid concentrations in JGM-S-treated females relative to controls. We infer FAS provides critically needed fatty acids to support JGM-enhanced fecundity in BPH.
authors.affiliation: School of Plant Protection, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Plant Protection, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Plant Protection, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Zongyu
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Plant Protection, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Linlin
authors.initials: L
authors.lastname: You
authors.affiliation: School of Plant Protection, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: You
authors.initials: Y
authors.lastname: Wu
authors.affiliation: School of Plant Protection, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.affiliation: School of Plant Protection, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Linquan
authors.initials: L
authors.lastname: Ge
authors.affiliation: Biological Control of Insects Research Laboratory, USDA/Agricultural Research Service, 1503 S. Providence Road, Columbia MO 65203, USA.
authors.forename: David
authors.initials: D
authors.lastname: Stanley
authors.affiliation: Division of Plant Sciences, University of Missouri, 1-31 Agriculture Building, Columbia, MO 65211, USA.
authors.forename: Qisheng
authors.initials: Q
authors.lastname: Song
authors.affiliation: School of Plant Protection, Yangzhou University, Yangzhou 225009, PR China. Electronic address: jincaiwu1952@sina.com.
authors.forename: Jincai
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry*; D001835:Body Weight; D005216:Fat Body / Q000187:drug effects; D064429:Fatty Acid Synthases / Q000378:metabolism*; D005260:Female; D005298:Fertility / Q000187:drug effects*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020868:Gene Silencing; D006430:Hemiptera / Q000187:drug effects / Q000502:physiology*; D007294:Inositol / Q000031:analogs & derivatives* / Q000737:chemistry; D019476:Insect Proteins / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry; D012275:Oryza / Q000382:microbiology; D010053:Ovary / Q000187:drug effects; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000737:chemistry
chemical_list: D000935:Antifungal Agents; D019476:Insect Proteins; D008055:Lipids; D012330:RNA, Double-Stranded; C030790:validamycin A; D007294:Inositol; D064429:Fatty Acid Synthases
keywords: Antibiotic jinggangmycin; Brown planthopper; FAS silencing; Fatty acids; ITRAQ analysis; Reproduction