title: CoenzymeQ10 localizations in model membranes. A Langmuir monolayer study.
abstract: The interaction of coenzyme Q10 (CoQ10) in a monolayer of 1,2-dipalmitoyl-sn-glysero-3-phospho-L-choline (DPPC), in a monolayer of 1,2-dierucoyl-sn-glysero-3-phospho-L-choline (DEPC), in a monolayer of 1-palmitoyl-2-oleoyl-sn-glysero-3-phospho-L-serine (POPS) and in a monolayer of total lipid extract from pig brain (PB) has been investigated by using the Langmuir monolayer technique. Surface pressure (π)-mean molecular area (mma) isotherms have been measured for pure lipid monolayers and lipid monolayers with 0.5, 1.0, 2.0, 5.0 and 10.0 mol% CoQ10 concentrations. At the biological concentration (1.0-3.0 mol%) of CoQ10, intercalation of CoQ10 occurs in the lipid acyl chains of DPPC, POPS and PB monolayers. Above the biological concentration of CoQ10, the CoQ10 molecule induces domain formation in the monolayers of DPPC, POPS and PB lipids. The DEPC monolayer behavior deviates from the other lipids in this study. At 2.0 mol% the CoQ10 promotes very dense lipid packing, and the CoQ10 molecule is located parallel to the DEPC acyl chains at all concentrations.
authors.affiliation: Department of Chemistry, University of Bergen, Allégaten 41, N-5007 Bergen, Norway.
authors.forename: Willy
authors.initials: W
authors.lastname: Nerdal
authors.affiliation: Department of Chemistry, University of Bergen, Allégaten 41, N-5007 Bergen, Norway.
authors.forename: Torill Regine Sandvik
authors.initials: TR
authors.lastname: Nilsen
authors.affiliation: Department of Biomedical Laboratory Science, Bergen University College, Inndalsveien 28, N-5020 Bergen, Norway. Electronic address: sst@hib.no.
authors.forename: Signe
authors.initials: S
authors.lastname: Steinkopf
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D010718:Phosphatidylserines / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D013552:Swine; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
chemical_list: D010718:Phosphatidylserines; D014451:Ubiquinone; D015060:1,2-Dipalmitoylphosphatidylcholine; C050294:1-palmitoyl-2-oleoylglycero-3-phosphoserine; C024989:coenzyme Q10
keywords: DEPC; DPPC; Monolayer; PB; POPS