title: Structural characterization, antioxidant and in vitro cytotoxic properties of seagrass, Cymodocea serrulata (R.Br.) Asch. & Magnus mediated silver nanoparticles.
abstract: The present study pertains to the synthesis, structural elucidation, antioxidant and in vitro cytotoxic properties of silver nanoparticles (AgNPs) from marine angiosperm, Cymodocea serrulata aqueous extract (CSAE). The characterization was made through UV-Visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), zeta potential and dynamic light scanning (DLS) analyses. The UV-Vis spectrum resulted in a strong surface plasmon resonance (SPR) at 430 nm. The average crystalline size of the AgNPs was predicted through XRD peaks that indicated the 2 theta values of 37.84°, 44.06°, 64.42° and 77.74° for Bragg's refraction index. The functional groups responsible for the bio-reduction of Ag(+) into Ag(0) were focused through FTIR spectrum. The FESEM images showed that the C. serrulata mediated AgNPs (CS-AgNPs) were spherical in shape. DPPH assay revealed the higher free radical scavenging activity in CS-AgNPs, when compared to CSAE. The cytotoxicity assay on the cervical cancer (HeLa) and African green monkey kidney (Vero) cells upon treatment with CSAE: 107.7 & 124.3 μgml(-1) and CS-AgNPs: 34.5 & 61.24 μgml(-1), respectively showed good inhibition rate. These findings highlight the fact that C. serrulata could be a potential source for developing potent drugs and further studies are needed.
authors.affiliation: Department of Biotechnology, School of Biosciences, Periyar University, Periyar Palkalai Nagar, Salem 636 011, India.
authors.forename: Abdhul Basheer
authors.initials: AB
authors.lastname: Chanthini
authors.affiliation: Department of Biotechnology, School of Biosciences, Periyar University, Periyar Palkalai Nagar, Salem 636 011, India.
authors.forename: Govindasamy
authors.initials: G
authors.lastname: Balasubramani
authors.affiliation: Department of Biotechnology, Padmavani Arts and Science College for Women, Salem 636 011, India.
authors.forename: Rajendiran
authors.initials: R
authors.lastname: Ramkumar
authors.affiliation: Department of Biotechnology, School of Biosciences, Periyar University, Periyar Palkalai Nagar, Salem 636 011, India.
authors.forename: Rajamani
authors.initials: R
authors.lastname: Sowmiya
authors.affiliation: Centre for Advanced Studies in Botany, School of Life Sciences, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: Manickam Dakshinamoorthi
authors.initials: MD
authors.lastname: Balakumaran
authors.affiliation: Centre for Advanced Studies in Botany, School of Life Sciences, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: Pudhupalayam Thangavelu
authors.initials: PT
authors.lastname: Kalaichelvan
authors.affiliation: Department of Biotechnology, School of Biosciences, Periyar University, Periyar Palkalai Nagar, Salem 636 011, India. Electronic address: perumalarticles@gmail.com.
authors.forename: Pachiappan
authors.initials: P
authors.lastname: Perumal
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002522:Chlorocebus aethiops; D000067493:Dynamic Light Scattering; D006367:HeLa Cells; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010936:Plant Extracts / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D020349:Surface Plasmon Resonance; D014709:Vero Cells; D014961:X-Ray Diffraction
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D012834:Silver
keywords: AgNPs; Cymodocea serrulata; Cytotoxicity; DPPH; FESEM