title: Preameloblast-Derived Factors Mediate Osteoblast Differentiation of Human Bone Marrow Mesenchymal Stem Cells by Runx2-Osterix-BSP Signaling.
abstract: Epithelial-mesenchymal interaction occurs during development of various tissues, including teeth and bone. Recently, a preameloblast-conditioned medium (PA-CM) from mouse apical bud cells (ABCs), a type of dental epithelial cell, was found to induce odontogenic differentiation of dental pulp stem cells and promote dentin formation. The aims of the present study were to investigate the effects of PA-CM on human bone marrow mesenchymal stem cells (hBMSCs) in vitro, and to investigate the bone regenerative capacity in vivo through epithelial-mesenchymal interactions of developmental osteogenesis. Coculturing with ABCs and PA-CM treatment upregulated osteoblast differentiation markers of hBMSCs compared to cells cultured alone. PA-CM accelerated mineralized nodule formation and also increased bone sialoprotein promoter activity in hBMSCs. PA-CM facilitated the migration of hBMSCs, but did not significantly influence proliferation. PA-CM promoted bone formation of hBMSCs in vivo. Radiographic and histologic findings showed that PA-CM induced the bony regeneration at calvarial defects in rat. Taken together, these data show that PA-CM enhances the migration and osteogenic differentiation of hBMSCs in vitro and induces bone formation in vivo.
authors.affiliation: 1 Department of Oral Histology and Developmental Biology, Seoul National University , Seoul, Korea.
authors.forename: Han-Wool
authors.initials: HW
authors.lastname: Choung
authors.affiliation: 1 Department of Oral Histology and Developmental Biology, Seoul National University , Seoul, Korea.
authors.forename: Dong-Seol
authors.initials: DS
authors.lastname: Lee
authors.affiliation: 1 Department of Oral Histology and Developmental Biology, Seoul National University , Seoul, Korea.
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Lee
authors.affiliation: 2 Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University , Seoul, Korea.
authors.forename: Won-Jun
authors.initials: WJ
authors.lastname: Shon
authors.affiliation: 1 Department of Oral Histology and Developmental Biology, Seoul National University , Seoul, Korea.
authors.forename: Joo-Cheol
authors.initials: JC
authors.lastname: Park
mesh_terms: D000565:Ameloblasts / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects*; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D017077:Culture Media, Conditioned; D058574:Integrin-Binding Sialoprotein; C493709:RUNX2 protein, human; D000076182:Sp7 Transcription Factor; C478163:SP7 protein, human; D014157:Transcription Factors