title: Development of a bioreactor based on magnetically stabilized fluidized bed for bioartificial liver.
abstract: Bioartificial liver (BAL) based on microcapsules has been proposed as a potential treatment for acute liver failure. The bioreactors used in such BAL are usually expected to achieve sufficient flow rate and minimized void volume for effective application. Due to the superiorities in bed pressure drop and operation velocity, magnetically stabilized fluidized beds (MSFBs) show the potential to serve as ideal microcapsule-based bioreactors. In the present study, we attempted to develop a microcapsule-based MSFB bioreactor for bioartificial liver device. Compared to conventional-fluidized bed bioreactors, the bioreactor presented here increased perfusion velocity and decreased void volume significantly. Meanwhile, the mechanical stability as well as the immunoisolation property of magnetite microcapsules were well maintained during the fluidization. Besides, the magnetite microcapsules were found no toxicity to cell survival. Therefore, our study might provide a novel approach for the design of microcapsule-based bioartificial liver bioreactors.
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Deng
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China. zhangying@dicp.ac.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Shen
authors.initials: S
authors.lastname: Li
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China. maxj@dicp.ac.cn.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ma
mesh_terms: D019149:Bioreactors; D052203:Ferrosoferric Oxide / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D019164:Liver, Artificial
chemical_list: D052203:Ferrosoferric Oxide
keywords: Bioartificial liver bioreactor; Cytotoxicity; Magnetically stabilized fluidized bed; Mechanical stability; Microcapsules