title: An UHPLC-MS/MS method for the simultaneous determination of ampicillin and sulbactam in human plasma and urine.
abstract: BACKGROUND
  With few new antibiotics available and an increased prevalence of multidrug-resistant bacteria, optimizing the effectiveness of currently available antibiotics and minimizing the potential for emerging resistance, through PK characterization, is of paramount importance.
  
  
  RESULTS
  Inter- and intra-assay results for ampicillin were within 5% for accuracy and 7% for precision, and for sulbactam within 7% for accuracy and 7% for precision, for both plasma and urine. The plasma Cmax was 38.4 µg/ml for ampicillin and 19.0 µg/ml for sulbactam. The urine concentrations were 668 µg/ml for ampicillin and 438 µg/ml for sulbactam.
  
  
  CONCLUSIONS
  An UHPLC-MS/MS method to simultaneously measure co-formulated ampicillin and sulbactam in plasma and urine, for use in a patient PK study, has been developed and validated.
authors.affiliation: Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.
authors.forename: Suzanne L
authors.initials: SL
authors.lastname: Parker
authors.affiliation: University of Queensland Centre for Clinical Research, The University of Queensland, Brisbane, Australia.
authors.forename: Syamhanin
authors.initials: S
authors.lastname: Adnan
authors.affiliation: Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.
authors.forename: Jenny L
authors.initials: JL
authors.lastname: Ordóñez Meija
authors.affiliation: University of Queensland Centre for Clinical Research, The University of Queensland, Brisbane, Australia.
authors.forename: David L
authors.initials: DL
authors.lastname: Paterson
authors.affiliation: Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.|Department of Intensive Care Medicine, Royal Brisbane & Women's Hospital, Brisbane, Australia.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Lipman
authors.affiliation: Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.|Department of Pharmacy, Royal Brisbane & Women's Hospital, Brisbane, Australia.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Roberts
authors.affiliation: Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.
authors.forename: Steven C
authors.initials: SC
authors.lastname: Wallis