title: Synthesis of water suitable as the MEPC.174(58) G8 influent water for testing ballast water management systems.
abstract: Here, we describe the methodologies adopted to ensure that natural seawater, used as "influent water" for the land test, complies with the requirement that should be fulfilled to show the efficacy of the new ballast water treatment system (BWTS). The new BWTS was located on the coast of SW Sicily (Italy), and the sampled seawater showed that bacteria and plankton were two orders of magnitude lower than requested. Integrated approaches for preparation of massive cultures of bacteria (Alcanivorax borkumensis and Marinobacter hydrocarbonoclasticus), algae (Tetraselmis suecica), rotifers (Brachionus plicatilis), and crustaceans (Artemia salina) suitable to ensure that 200 m(3) of water fulfilled the international guidelines of MEPC.174(58)G8 are here described. These methodologies allowed us to prepare the "influent water" in good agreement with guidelines and without specific problems arising from natural conditions (seasons, weather, etc.) which significantly affect the concentrations of organisms at sea. This approach also offered the chance to reliably run land tests once every two weeks.
authors.affiliation: Institute for Coastal Marine Environment (IAMC) - CNR of Capo Granitola, Via del Mare 3, Torretta Granitola (TP), 91021, Italy. fabio.dagostino@iamc.cnr.it.
authors.forename: Fabio
authors.initials: F
authors.lastname: D'Agostino
authors.affiliation: Institute for Coastal Marine Environment (IAMC) - CNR of Capo Granitola, Via del Mare 3, Torretta Granitola (TP), 91021, Italy.
authors.forename: Marianna
authors.initials: M
authors.lastname: Del Core
authors.affiliation: Institute for Coastal Marine Environment (IAMC) - CNR of Messina, Via Spianata San Raineri 86, Messina, 98122, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Cappello
authors.affiliation: Institute for Coastal Marine Environment (IAMC) - CNR of Capo Granitola, Via del Mare 3, Torretta Granitola (TP), 91021, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Mazzola
authors.affiliation: Institute for Coastal Marine Environment (IAMC) - CNR of Capo Granitola, Via del Mare 3, Torretta Granitola (TP), 91021, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Sprovieri
mesh_terms: D053521:Alcanivoraceae / Q000254:growth & development; D000818:Animals; D001156:Artemia / Q000254:growth & development; D000460:Chlorophyta / Q000254:growth & development; D002934:Cinnamates / Q000737:chemistry*; D004784:Environmental Monitoring / Q000379:methods; D004867:Equipment Design; D017408:Guidelines as Topic; D012403:Rotifera / Q000254:growth & development; D012623:Seawater / Q000032:analysis / Q000382:microbiology; D012767:Ships / Q000592:standards*; D012802:Sicily; D018508:Water Purification / Q000295:instrumentation / Q000379:methods*
chemical_list: C570869:2-methylphenyl cinnamate; D002934:Cinnamates
keywords: Ballast water treatment system; Culture organisms; Influent waters; Land test; MEPC.174(58)G8