title: Comparative quantitative trait loci for silique length and seed weight in Brassica napus.
abstract: Silique length (SL) and seed weight (SW) are important yield-associated traits in rapeseed (Brassica napus). Although many quantitative trait loci (QTL) for SL and SW have been identified in B. napus, comparative analysis for those QTL is seldom performed. In the present study, 20 and 21 QTL for SL and SW were identified in doubled haploid (DH) and DH-derived reconstructed F2 populations in rapeseed, explaining 55.1-74.3% and 24.4-62.9% of the phenotypic variation across three years, respectively. Of which, 17 QTL with partially or completely overlapped confidence interval on chromosome A09, were homologous with two overlapped QTL on chromosome C08 by aligning QTL confidence intervals with the reference genomes of Brassica crops. By high density selective genotyping of DH lines with extreme phenotypes, using a Brassica single-nucleotide polymorphism (SNP) array, the QTL on chromosome A09 was narrowed, and aligned into 1.14-Mb region from 30.84 to 31.98 Mb on chromosome R09 of B. rapa and 1.05-Mb region from 27.21 to 28.26 Mb on chromosome A09 of B. napus. The alignment of QTL with Brassica reference genomes revealed homologous QTL on A09 and C08 for SL. The narrowed QTL region provides clues for gene cloning and breeding cultivars by marker-assisted selection.
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.|Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
authors.forename: Ying
authors.initials: Y
authors.lastname: Fu
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Wei
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Dong
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: He
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Cui
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Jiaqin
authors.initials: J
authors.lastname: Mei
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Huafang
authors.initials: H
authors.lastname: Wan
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Jiana
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
authors.forename: Rod
authors.initials: R
authors.lastname: Snowdon
authors.affiliation: Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Friedt
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qian
mesh_terms: D029688:Brassica napus / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping; D005838:Genotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D012639:Seeds / Q000235:genetics* / Q000502:physiology*