title: Bovine Osteochondral Tissues: A Questionable Model to Evaluate Mechanical Loading In Vitro.
abstract: Articular cartilage exists within synovial joints to adsorb and distribute mechanical loads to the subchondral bone. Mechanical loading is one aspect of a wide range of microenvironmental stressors that contribute to the maintenance of articular cartilage. The aim of the current study was to characterize bovine osteochondral tissues and to assess their suitability to serve as a model for investigating the effects of mechanical loading on cartilage tissue in vitro using a custom-made reactor system. Osteochondral tissues were harvested from bovine knee joints and cultured up to 24 days in loaded and unloaded conditions. Notably, we found a considerable zone-specific heterogeneity between cartilage explants harvested from the same joint as evidenced by histology and gene expression levels. Results using the reactor system revealed that differences observed after mechanical loading varied within the range of the heterogeneity observed amongst the different cartilage explants. Thus, it may be difficult to obtain reliable and reproducible data in mechanical loading experiments from these tissues in vitro, especially in cases where small variations between the experimental groups are expected. This will likely lead to the reporting of false positives or negatives in studies investigating the effect of mechanical load on the function of cartilage tissue.
authors.forename: Adel
authors.initials: A
authors.lastname: Tekari
authors.forename: Reto
authors.initials: R
authors.lastname: Luginbuehl
authors.forename: Willy
authors.initials: W
authors.lastname: Hofstetter
authors.forename: Rainer J
authors.initials: RJ
authors.lastname: Egli
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000502:physiology*; D002417:Cattle; D019245:Compressive Strength / Q000502:physiology*; D066298:In Vitro Techniques; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D023421:Models, Animal; D009924:Organ Culture Techniques / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D033081:Shear Strength / Q000502:physiology; D013314:Stress, Mechanical; D013718:Tensile Strength / Q000502:physiology; D016474:Weight-Bearing / Q000502:physiology*