title: Engineering large animal models of human disease.
abstract: The recent development of gene editing tools and methodology for use in livestock enables the production of new animal disease models. These tools facilitate site-specific mutation of the genome, allowing animals carrying known human disease mutations to be produced. In this review, we describe the various gene editing tools and how they can be used for a range of large animal models of diseases. This genomic technology is in its infancy but the expectation is that through the use of gene editing tools we will see a dramatic increase in animal model resources available for both the study of human disease and the translation of this knowledge into the clinic. Comparative pathology will be central to the productive use of these animal models and the successful translation of new therapeutic strategies.
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Science, Easter Bush Campus, University of Edinburgh, Edinburgh, EH25 9RG, UK.
authors.forename: C Bruce A
authors.initials: CB
authors.lastname: Whitelaw
authors.affiliation: Animal Bioscience and Biotechnology Laboratory, ARS, Beltsville, MD, 20705, USA.|Department of Animal and Avian Sciences, Beltsville, MD, 20742, USA.
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Sheets
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Science, Easter Bush Campus, University of Edinburgh, Edinburgh, EH25 9RG, UK.
authors.forename: Simon G
authors.initials: SG
authors.lastname: Lillico
authors.affiliation: Animal Bioscience and Biotechnology Laboratory, ARS, Beltsville, MD, 20705, USA.|Department of Animal and Avian Sciences, Beltsville, MD, 20742, USA.
authors.forename: Bhanu P
authors.initials: BP
authors.lastname: Telugu
mesh_terms: D000483:Alleles; D000818:Animals; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics; D004195:Disease Models, Animal; D033041:Embryo Research; D005544:Forecasting; D016368:Frameshift Mutation / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods* / Q000639:trends; D006801:Humans; D009154:Mutation / Q000235:genetics*; D053652:Nuclear Transfer Techniques; D011323:Primates; D013552:Swine; D015533:Transcriptional Activation / Q000235:genetics; D016335:Zinc Fingers / Q000235:genetics
keywords: CRISPR; SCNT; TALEN; ZFN; gene editing; livestock; pathology; pigs; zygote