title: Development of M2BPGi: a novel fibrosis serum glyco-biomarker for chronic hepatitis/cirrhosis diagnostics.
abstract: Many proteins in the living body are glycoproteins, which present glycans linked on their surface. Glycan structures reflect the degree of cell differentiation or canceration and are cell specific. These characteristics are advantageous in the development of various disease biomarkers. Glycoprotein-based biomarkers (glyco-biomarkers) are developed by utilizing the specific changes in the glycan structure on a glycoprotein secreted from the diseased cells of interest. Therefore, quantification of the altered glycan structures is the key to developing a new glyco-biomarker. Glycoscience is a relatively new area of molecular science, and recent advancement of glycotechnologies is remarkable. In the author's institute, new glycoscience technologies have been designed to be efficiently utilized for the development of new diagnostic agents. This paper introduces a strategy for glyco-biomarker development, which was successfully applied in the development of Wisteria floribunda agglutinin-positive Mac-2 binding protein M2BPGi, a liver fibrosis marker now commercially available for clinical use.
authors.affiliation: a Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST), Central 2, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8568, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000097:blood; D054794:Glycomics / Q000379:methods; D006023:Glycoproteins / Q000097:blood* / Q000737:chemistry; D006505:Hepatitis / Q000097:blood*; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood*; D008969:Molecular Sequence Data
chemical_list: D015415:Biomarkers; D006023:Glycoproteins
keywords: IGOT-LC/MS; M2BPGi; WFA-positive Mac-2 binding protein; cirrhosis; glyco-biomarker; glycoscience; hepatitis; lectin microarray; liver fibrosis; translational research