title: Culturing of Mouse Mesenchymal Stem Cells on Poly-3-Hydroxybutyrate Scaffolds.
abstract: We studied the possibility of long-term culturing of mouse mesenchymal stem cells on a porous scaffold made of biocompatible polymer poly-3-hydroxybutyrate. The cells remained viable for at least 2 months and passed more than 65 population doublings in culture. Culturing on the scaffold did not change surface phenotype of cells. 3D poly-3-hydroxybutyrate scaffolds are appropriate substrate for long-term culturing of mesenchymal stem cells.
authors.affiliation: V. A. Engelhard Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.|Department of Bioengineering, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Andreeva
authors.affiliation: Department of Bioengineering, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia. ant_bonar@mail.ru.|Federal Research Centre Fundamentals of Biotechnology, Russian Academy of Sciences, Moscow, Russia. ant_bonar@mail.ru.
authors.forename: A P
authors.initials: AP
authors.lastname: Bonartsev
authors.affiliation: Department of Bioengineering, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: I I
authors.initials: II
authors.lastname: Zharkova
authors.affiliation: Federal Research Centre Fundamentals of Biotechnology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: T K
authors.initials: TK
authors.lastname: Makhina
authors.affiliation: Federal Research Centre Fundamentals of Biotechnology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: V L
authors.initials: VL
authors.lastname: Myshkina
authors.affiliation: Physical Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: E P
authors.initials: EP
authors.lastname: Kharitonova
authors.affiliation: Department of Bioengineering, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia.|Federal Research Centre Fundamentals of Biotechnology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Voinova
authors.affiliation: Physical Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: G A
authors.initials: GA
authors.lastname: Bonartseva
authors.affiliation: Department of Bioengineering, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: K V
authors.initials: KV
authors.lastname: Shaitan
authors.affiliation: V. A. Engelhard Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Belyavskii
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D006885:Hydroxybutyrates / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008810:Mice, Inbred C57BL; D011091:Polyesters / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: mesenchymal stem cells; poly-3-hydroxybutyrate; polymer scaffold