title: Aerobic cyanide degradation by bacterial isolates from cassava factory wastewater.
abstract: Ten bacterial strains that utilize cyanide (CN) as a nitrogen source were isolated from cassava factory wastewater after enrichment in a liquid media containing sodium cyanide (1 mM) and glucose (0.2% w/v). The strains could tolerate and grow in cyanide concentrations of up to 5 mM. Increased cyanide levels in the media caused an extension of lag phase in the bacterial growth indicating that they need some period of acclimatisation. The rate of cyanide removal by the strains depends on the initial cyanide and glucose concentrations. When initial cyanide and glucose concentrations were increased up to 5 mM, cyanide removal rate increased up to 63 and 61 per cent by Bacillus pumilus and Pseudomonas putida. Metabolic products such as ammonia and formate were detected in culture supernatants, suggesting a direct hydrolytic pathway without an intermediate formamide. The study clearly demonstrates the potential of aerobic treatment with cyanide degrading bacteria for cyanide removal in cassava factory wastewaters.
authors.affiliation: Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, India .
authors.forename: Sujatha
authors.initials: S
authors.lastname: Kandasamy
authors.affiliation: Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, India .
authors.forename: Balachandar
authors.initials: B
authors.lastname: Dananjeyan
authors.affiliation: Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, India .
authors.forename: Kumar
authors.initials: K
authors.lastname: Krishnamurthy
authors.affiliation: Institute of Applied Microbiology, Justus Liebig University, Giessen, Germany .
authors.forename: Gero
authors.initials: G
authors.lastname: Benckiser
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001407:Bacillus / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D003486:Cyanides / Q000378:metabolism*; D005561:Formates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007194:India; D002365:Manihot; D016958:Pseudomonas putida / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D062065:Wastewater / Q000737:chemistry*
chemical_list: D000641:Ammonia; D003486:Cyanides; D005561:Formates; D005947:Glucose; D012336:RNA, Ribosomal, 16S; D062065:Wastewater
keywords: aerobic degradation; cassava factory wastewater; cyanide degrading bacteria