title: Reductive Dehalogenases Come of Age in Biological Destruction of Organohalides.
abstract: Halogenated organic compounds (organohalides) are globally prevalent, recalcitrant toxic, and carcinogenic environmental pollutants. Select microorganisms encode enzymes known as reductive dehalogenases (EC 1.97.1.8) that catalyze reductive dehalogenation reactions resulting in the generation of lesser-halogenated compounds that may be less toxic and more biodegradable. Recent breakthroughs in enzyme structure determination, elucidation of the mechanisms of reductive dehalogenation, and in heterologous expression of functional reductive dehalogenase enzymes have substantially increased our understanding of this fascinating class of enzymes. This knowledge has created opportunities for more versatile (in situ and ex situ) biologically-mediated organohalide destruction strategies.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, 2052, Australia.
authors.forename: Bat-Erdene
authors.initials: BE
authors.lastname: Jugder
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, 2052, Australia; Department of Molecular Biology and Genetics, Istanbul University, Istanbul, Turkey.
authors.forename: Haluk
authors.initials: H
authors.lastname: Ertan
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, 2052, Australia.
authors.forename: Matthew
authors.initials: M
authors.lastname: Lee
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, 2052, Australia.
authors.forename: Michael
authors.initials: M
authors.lastname: Manefield
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, 2052, Australia. Electronic address: c.marquis@unsw.edu.au.
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Marquis
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D055162:Biocatalysis; D001673:Biodegradation, Environmental; D020134:Catalytic Domain; D041862:Chloroflexi / Q000201:enzymology / Q000235:genetics; D000068016:Eubacteriales / Q000201:enzymology / Q000235:genetics; D045858:Desulfitobacterium / Q000201:enzymology / Q000235:genetics; D004785:Environmental Pollutants / Q000737:chemistry / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006846:Hydrocarbons, Halogenated / Q000737:chemistry / Q000378:metabolism*; D006867:Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006868:Hydrolysis; D008958:Models, Molecular; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004785:Environmental Pollutants; D006846:Hydrocarbons, Halogenated; D011994:Recombinant Proteins; D006867:Hydrolases
keywords: Dehalobacter; Dehalococcoides; bioremediation; organohalide respiration; reductive dehalogenase