title: [Protective effects of histone deacetylase inhibitor on stress-induced myocardial injury in rats].
abstract: OBJECTIVE
  To observe the protective effects of histone deacetylase inhibitor on stress-induced myocardial injury.
  
  
  METHODS
  Healthy male Wistar rats were randomly divided into 3 groups( n = 6), and the stress-induced myocardial injury model was established with chronic restraint stress method. The protective effects of histone deacetylase inhibitor on stress-induced myocardial injury were observed with Trichostatin A (TSA) intervention. Histone acetylation levels in myocardium of rats were detected by Western blot method, spectrophotometry method was used to dynamically determine the activity of rat serum lactate dehydrogenase (LDH), serum creatine kinase isoenzyme-MB (CK-MB) and Caspase 3, and nagar Olsen staining were used to observe the early myocardial damage.
  
  
  RESULTS
  Restraint stress could significantly reduce the level of histone acetylation of myocardium in rats, and TSA intervention could inhibit the stress-induced reduction of myocardial levels of histone acetylation. Restraint stress could cause the significant increase of serum LDH activity ( P < 0.05), serum CK-MB activity ( P < 0.05), and the Caspase 3 activity of myocardial tissue (P < 0.05), and early myocardial damage also occurred during restraint stress. ISA intervention could significantly reduce the serum LDH activity (P < 0.05), the serum CK-MB activity (P < 0.05), the activity of myocardial tissue caspase 3 induced by restraint stress (P < 0.05), and the stress-induced myocardial injury was also attenuated by TSA intervention.
  
  
  CONCLUSIONS
  The histone deacetylase inhibitor TSA can protect stress-induced myocardial injury.
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Liu
authors.forename: Tian-hui
authors.initials: TH
authors.lastname: Wang
authors.forename: Rui-feng
authors.initials: RF
authors.lastname: Duan
authors.forename: Xue-si
authors.initials: XS
authors.lastname: Zhou
authors.forename: Hong-tao
authors.initials: HT
authors.lastname: Liu
authors.forename: Zhi-qing
authors.initials: ZQ
authors.lastname: Zhang
mesh_terms: D000107:Acetylation; D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D053148:Caspase 3 / Q000097:blood; D052279:Creatine Kinase, MB Form / Q000097:blood; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D009206:Myocardium / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013312:Stress, Physiological
chemical_list: D002316:Cardiotonic Agents; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C012589:trichostatin A; D007770:L-Lactate Dehydrogenase; D052279:Creatine Kinase, MB Form; C505410:Casp3 protein, rat; D053148:Caspase 3