title: Preparation and characterization of silver nanoparticles on silk fibroin/carboxymethylchitosan composite sponge as anti-bacterial wound dressing.
abstract: The infection in burn wounds covered by biologic dressings leads to wound deepening and chronic wounds. The introduction of silver nanoparticles (AgNPs) into biologic dressings is a beneficial method to prevent wound infection and simultaneously promote wound healing. In this study, an AgNP-loaded silk fibroin (SF)/carboxymethylchitosan (CMC) composite sponge was fabricated. AgNPs with a mean diameter of 4.9 nm was synthesized in SF solution in situ. While CMC was incorporated and chemically crosslinked, SF was insolubilized by ethanol annealing. SEM imaging determined that the AgNP-loaded SF/CMC sponge was more porous than the pure SF sponge. Anti-bacterial results, measured by disk-diffusion and bacterial suspension assay, showed that the AgNP-loaded SF/CMC sponge demonstrated effective anti-bacterial activity against S. aureus and P. aeruginosa, and that its anti-P. aeruginosa activity was higher than that of AQUACEL®; Ag. The introduction of CMC improved the water absorption capacity, retention ability, and water vapor transmission rate of the sponge, which are all important properties of wound dressings.
authors.affiliation: Department of Pharmacy, Wuxi No. 2 People's Hospital, Wuxi 214002, P.R. China.
authors.forename: Zejun
authors.initials: Z
authors.lastname: Pei
authors.affiliation: Department of Pharmacy, Wuxi No. 2 People's Hospital, Wuxi 214002, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Pharmacy, Wuxi No. 2 People's Hospital, Wuxi 214002, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Pharmacy, Wuxi No. 2 People's Hospital, Wuxi 214002, P.R. China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Central Laboratory, Wuxi Third People's Hospital, Wuxi 214122, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
mesh_terms: D065966:Absorption, Physicochemical; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D001458:Bandages; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000138:chemical synthesis; D004058:Diffusion; D005349:Fibroins / Q000737:chemistry*; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D012834:Silver / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; C514968:carboxymethyl-chitosan; D012834:Silver; D005349:Fibroins; D048271:Chitosan
keywords: Silver nanoparticles; carboxymethylchitosan; silk fibroin; wound healing; wound infection