title: Performance of peanut mutants and their offspring generated from mixed high-energy particle field radiation and tissue culture.
abstract: To develop new ways to breed peanut, we irradiated seeds of the Luhua 11 cultivar with a mixed high-energy particle field at different doses. The embryonic leaflets were extracted as explants and incubated on somatic embryo induction medium and then on somatic embryo germination and regeneration medium. After being grafted, the M1-generation plants were transplanted, and seeds from each M1-generation plant were harvested. In the following year, the M2-generation seeds were planted separately. Some M2-generation plants showed distinct character segregation relative to the mutagenic parent in terms of vigor, fertility, plant height, branch number, and pod size and shape. M2-generation plants that had a high pod weight per plant tended to produce M3-generation offspring that also had a high pod weight per plant, much higher than that of the mutagenic parent, Luhua 11. M4-generation seeds varied greatly in quality, and 35 individuals with an increased fat content (>55%) were obtained. Overall, the results indicate that the combination of mutagenesis via mixed high-energy particle field exposure and tissue culture is promising for peanut breeding.
authors.affiliation: Key Laboratory of Plant Biotechnology in Universities of Shandong, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, College of Life Science, Qingdao Agricultural University, Qingdao, China.
authors.forename: J S
authors.initials: JS
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Biotechnology in Universities of Shandong, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, College of Life Science, Qingdao Agricultural University, Qingdao, China.
authors.forename: L X
authors.initials: LX
authors.lastname: Qiao
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, China Academy of Agricultural Sciences, National Center of Space Mutagenesis for Crop Improvement, Beijing, China.
authors.forename: L S
authors.initials: LS
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Plant Biotechnology in Universities of Shandong, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, College of Life Science, Qingdao Agricultural University, Qingdao, China.
authors.forename: P
authors.initials: P
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Biotechnology in Universities of Shandong, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, College of Life Science, Qingdao Agricultural University, Qingdao, China.
authors.forename: B T
authors.initials: BT
authors.lastname: Guo
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, China Academy of Agricultural Sciences, National Center of Space Mutagenesis for Crop Improvement, Beijing, China.
authors.forename: L X
authors.initials: LX
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Biotechnology in Universities of Shandong, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, College of Life Science, Qingdao Agricultural University, Qingdao, China suijiongming@163.com.
authors.forename: J M
authors.initials: JM
authors.lastname: Sui
mesh_terms: D010367:Arachis / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects*; D018525:Germination / Q000235:genetics / Q000528:radiation effects; D009154:Mutation / Q000528:radiation effects; D010641:Phenotype; D000069600:Plant Breeding; D010937:Plant Growth Regulators / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects
chemical_list: D010937:Plant Growth Regulators