title: Toll-Like Receptors 2 and 4 Polymorphisms in Age-Related Macular Degeneration.
abstract: Age-related macular degeneration (AMD) is a complex disorder with multifactorial etiology, caused by a combination of genetic and environmental factors. Innate immunity appears to play a key role in the pathogenesis of AMD. The purpose of this study was to determine whether common variation in the human toll-like receptors (TLRs) 2 and 4 alters the risk of AMD.
  
  
  
  A total of 183 patients with AMD and 200 disease-free control subjects were enrolled. The genotyping of polymorphisms TLR2 (TLR2-Arg753Gln: rs5743708) and TLR4 (TLR4-Asp299Gly: rs4986790; TLR4-Thr399Ile: rs4986791) were done using real-time PCR.
  
  
  
  TLR2 Arg753Gln genotype had approximately four times greater risk of AMD compared with TLR2 Arg753Arg genotype (OR = 3.88; 95% CI: 1.76-8.75, p = 0.001). TLR2 Arg753Gln genotype was significantly higher in the patients with dry-type AMD (16%) and wet-type AMD (18%) than in the control (5%) subjects (p = 0.005 and p = 0.0008, respectively). There were no significant differences in the distribution of TLR4-Asp299Gly and TLR4-Thr399Ile genotypes between AMD patients and controls (p > 0.05).
  
  
  
  Our results suggest that TLR2 polymorphism may contribute to the pathogenesis of AMD.
authors.affiliation: a Department of Medical Biology, Cerrahpasa Faculty of Medicine , University of Istanbul , Istanbul , Turkey .
authors.forename: Mehmet
authors.initials: M
authors.lastname: Güven
authors.affiliation: a Department of Medical Biology, Cerrahpasa Faculty of Medicine , University of Istanbul , Istanbul , Turkey .
authors.forename: Bahadır
authors.initials: B
authors.lastname: Batar
authors.affiliation: a Department of Medical Biology, Cerrahpasa Faculty of Medicine , University of Istanbul , Istanbul , Turkey .
authors.forename: Tuba
authors.initials: T
authors.lastname: Mutlu
authors.affiliation: a Department of Medical Biology, Cerrahpasa Faculty of Medicine , University of Istanbul , Istanbul , Turkey .
authors.forename: Merve
authors.initials: M
authors.lastname: Bostancı
authors.affiliation: a Department of Medical Biology, Cerrahpasa Faculty of Medicine , University of Istanbul , Istanbul , Turkey .
authors.forename: Meltem
authors.initials: M
authors.lastname: Mete
authors.affiliation: b Department of Ophthalmology, Cerrahpasa Faculty of Medicine , University of Istanbul , Istanbul , Turkey and.
authors.forename: Cengiz
authors.initials: C
authors.lastname: Aras
authors.affiliation: c Department of Ophthalmology , Akdeniz University Medical Faculty , Antalya , Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ünal
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D004247:DNA / Q000235:genetics*; D005260:Female; D005451:Fluorescein Angiography; D005654:Fundus Oculi; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008268:Macular Degeneration / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D051195:Toll-Like Receptor 2 / Q000235:genetics* / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics* / Q000378:metabolism
chemical_list: D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D004247:DNA
keywords: Age-related macular degeneration; TLR polymorphism