title: Plant-based porcine reproductive and respiratory syndrome virus VLPs induce an immune response in mice.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) significantly affects the swine industry worldwide. An efficient, protective vaccine is still lacking. Here, we report for the first time the generation and purification of PRRSV virus like particles (VLPs) by expressing GP5, M and N genes in Nicotiana silvestris plants. The particles were clearly visible by transmission electron microscopy (TEM) with a size of 60-70 nm. Hydrodynamic diameter of the particles was obtained and it was confirmed that the VLPs had the appropriate size for PRRS virions and that the VLPs were highly pure. By measuring the Z potential we described the electrophoretic mobility behavior of VLPs and the best conditions for stability of the VLPs were determined. The particles were immunogenic in mice. A western blot of purified particles allowed detection of three coexpressed genes. These VLPs may serve as a platform to develop efficient PRRSV vaccines.
authors.affiliation: Departamento de Ingeniería Genética, CINVESTAV-IPN, Km 9.6 Carretera Irapuato-León, C.P. 36821 Irapuato, Guanajuato, México. Electronic address: uribecampero@yahoo.com.mx.
authors.forename: Laura
authors.initials: L
authors.lastname: Uribe-Campero
authors.affiliation: Unidad de Investigación Médica en Enfermedades Oncológicas, IMSS, CMN SXXI, México, D.F., México; Laboratorio de Inmunobiología, Lab, 3PB, Unidad de Investigación en Diferenciación Celular y Cáncer, Facultad de Estudios Superiores Zaragoza, UMIEZ, Campus II, UNAM, Batalla 5 de mayo s/n, Col. E. Oriente, Esquina Fuerte Loreto, Iztapalapa, CP 09230 México, D.F., México. Electronic address: albertomon@yahoo.com.
authors.forename: Alberto
authors.initials: A
authors.lastname: Monroy-García
authors.affiliation: Laboratorio de Física Biológica, Instituto de Física, Universidad Autónoma de San Luis Potosí, Álvaro Obregón 64, San Luis Potosí, SLP 78000, México. Electronic address: analuisa.duranmeza@gmail.com.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Durán-Meza
authors.affiliation: Laboratorio de Física Biológica, Instituto de Física, Universidad Autónoma de San Luis Potosí, Álvaro Obregón 64, San Luis Potosí, SLP 78000, México. Electronic address: veronica.villagrana@gmail.com.
authors.forename: María V
authors.initials: MV
authors.lastname: Villagrana-Escareño
authors.affiliation: Laboratorio de Física Biológica, Instituto de Física, Universidad Autónoma de San Luis Potosí, Álvaro Obregón 64, San Luis Potosí, SLP 78000, México. Electronic address: jaime@ifisica.uaslp.mx.
authors.forename: Jaime
authors.initials: J
authors.lastname: Ruíz-García
authors.affiliation: Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo A.C., Carretera a La Victoria km 0.6, Hermosillo, Sonora C.P. 83304, México. Electronic address: jhdez@ciad.mx.
authors.forename: Jesús
authors.initials: J
authors.lastname: Hernández
authors.affiliation: División de Ciencias de la Vida, Campus Irapuato-Salamanca, Universidad de Guanajuato, Exhacienda El Copal s/n, A.P. 311, Irapuato, Gto. C.P. 36500, México. Electronic address: palenius@ugto.mx.
authors.forename: Héctor G
authors.initials: HG
authors.lastname: Núñez-Palenius
authors.affiliation: Departamento de Ingeniería Genética, CINVESTAV-IPN, Km 9.6 Carretera Irapuato-León, C.P. 36821 Irapuato, Guanajuato, México. Electronic address: mgomez@ira.cinvestav.m.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Gómez-Lim
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D015153:Blotting, Western; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D051379:Mice; D019318:Porcine Reproductive and Respiratory Syndrome / Q000517:prevention & control / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D013552:Swine; D014026:Nicotiana / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D014765:Viral Vaccines
keywords: Plants; Porcine reproductive respiratory syndrome virus; Vaccine; Virus-like particles