title: Absence of population genetic structure in Heterakis gallinarum of chicken from Sichuan, inferred from mitochondrial cytochrome c oxidase subunit I gene.
abstract: Population genetics information provides a foundation for understanding the transmission and epidemiology of parasite and, therefore, may be used to assist in the control of parasitosis. However, limited available sequence information in Heterakis gallinarum has greatly impeded the study in this area. In this study, we first investigated the genetic variability and genetic structure of H. gallinarum. The 1325 bp fragments of the mitochondrial COX1 gene were amplified in 56 isolates of H. gallinarum from seven different geographical regions in Sichuan province, China. The 56 sequences were classified into 22 haplotypes (H1-H22). The values of haplotype diversity (0.712) and nucleotide diversity (0.00158) in Sichuan population indicate a rapid expansion occurred from a relatively small, short-term effective population in the past. The haplotype network formed a distribution around H1 in a star-like topology, and the haplotypes did not cluster according to their geographical location. Similar conclusions could be made from MP phylogenetic tree. The Fst value (Fst<0.16965) and AMOVA analysis revealed that no significant genetic differentiation was observed among the seven different geographical populations. Neutrality tests (Tajima's D and Fu's Fs) and mismatch analysis indicated that H. gallinarum experienced a population expansion in the past. Our results indicated that H. gallinarum experienced a rapid population expansion in the past, and there was a low genetic diversity and an absence of population structure across the population.
authors.affiliation: a Department of Parasitology , College of Veterinary Medicine, Sichuan Agricultural University , Chengdu , China .
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Gu
authors.affiliation: a Department of Parasitology , College of Veterinary Medicine, Sichuan Agricultural University , Chengdu , China .
authors.forename: Jun-Yang
authors.initials: JY
authors.lastname: Zhu
authors.affiliation: a Department of Parasitology , College of Veterinary Medicine, Sichuan Agricultural University , Chengdu , China .
authors.forename: Ke-Ling
authors.initials: KL
authors.lastname: Jian
authors.affiliation: a Department of Parasitology , College of Veterinary Medicine, Sichuan Agricultural University , Chengdu , China .
authors.forename: Bao-Jian
authors.initials: BJ
authors.lastname: Wang
authors.affiliation: b Department of Chemistry , College of Life and Basic Science, Sichuan Agricultural University , Ya'an , China , and.
authors.forename: Xue-Rong
authors.initials: XR
authors.lastname: Peng
authors.affiliation: a Department of Parasitology , College of Veterinary Medicine, Sichuan Agricultural University , Chengdu , China .
authors.forename: Guang-You
authors.initials: GY
authors.lastname: Yang
authors.affiliation: a Department of Parasitology , College of Veterinary Medicine, Sichuan Agricultural University , Chengdu , China .
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: a Department of Parasitology , College of Veterinary Medicine, Sichuan Agricultural University , Chengdu , China .
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Zhong
authors.affiliation: c Department of Science and Technology , Ganzi Institute of Scientific and Technological Information , Ganzi , China.
authors.forename: Ke-Yun
authors.initials: KY
authors.lastname: Peng
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D002645:Chickens / Q000235:genetics*; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D014644:Genetic Variation; D054629:Genome, Mitochondrial; D006239:Haplotypes; D010802:Phylogeny; D058974:Phylogeography; D017422:Sequence Analysis, DNA
chemical_list: D030161:Avian Proteins; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV
keywords: COX1 gene; Heterakis gallinarum; Sichuan; genetic variability