title: Early-shared Mycobacterium bovis bacillus Calmette-Guérin sub-strains induce Th1 cytokine production in vivo.
abstract: Interleukin-12 is one of the cytokines that induce acquired immunity by progressing the differentiation of T cells. When antigens are presented by APCs, including macrophages and DCs, T cells are activated and produce the Th1 cytokines IL-2 and IFN-γ. We have previously reported greater IL-12 production from macrophages infected with early-shared BCG sub-strains (ex. BCG-Japan, -Sweden) than from those infected with late-shared BCG (ex. BCG-Pasteur and -Connaught) . In this study, we investigated the Th1 cytokine-inducing activity of splenocytes co-cultured with BCG-infected DCs. Early-shared BCG-infected DCs produced IL-12 and TNF-α⋅ Furthermore, when they were co-cultured with purified protein derivative-stimulated DCs, the splenocytes of mice immunized with BCG-Tokyo/Japan produced more Th1 cytokine than did those of mice immunized with BCG-Connaught. In conclusion, early-shared BCG sub-strains more strongly induce Th1 cytokine production in vivo. This study provides basic information to inform the selection of candidates for primary vaccination.
authors.affiliation: Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe, Mizuho-ku, Nagoya 467-8603.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Taniguchi
authors.affiliation: Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe, Mizuho-ku, Nagoya 467-8603.
authors.forename: Yuuji
authors.initials: Y
authors.lastname: Miyatake
authors.affiliation: Japan BCG Laboratory, 3-1-5 Matsuyama, Kiyose, Tokyo 204-0022, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Hayashi
authors.affiliation: Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe, Mizuho-ku, Nagoya 467-8603.
authors.forename: Atsuro
authors.initials: A
authors.lastname: Takami
authors.affiliation: Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe, Mizuho-ku, Nagoya 467-8603.
authors.forename: Saotomo
authors.initials: S
authors.lastname: Itoh
authors.affiliation: Japan BCG Laboratory, 3-1-5 Matsuyama, Kiyose, Tokyo 204-0022, Japan.
authors.forename: Saburo
authors.initials: S
authors.lastname: Yamamoto
authors.affiliation: Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe, Mizuho-ku, Nagoya 467-8603.
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Hida
authors.affiliation: Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe, Mizuho-ku, Nagoya 467-8603.
authors.forename: Kikuo
authors.initials: K
authors.lastname: Onozaki
authors.affiliation: Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe, Mizuho-ku, Nagoya 467-8603.
authors.forename: Takemasa
authors.initials: T
authors.lastname: Takii
mesh_terms: D000818:Animals; D018920:Coculture Techniques; D016207:Cytokines / Q000096:biosynthesis*; D003713:Dendritic Cells / Q000502:physiology*; D005260:Female; D018664:Interleukin-12 / Q000096:biosynthesis*; D051379:Mice; D009165:Mycobacterium Infections, Nontuberculous / Q000503:physiopathology*; D009163:Mycobacterium bovis / Q000472:pathogenicity*; D018417:Th1 Cells / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12
keywords: BCG vaccines; IL-12; Th1