title: Molecular variability in the cysteine rich protein of potato virus M.
abstract: The potato virus M (PVM), belonging to the genus Carlavirus, is a worldwide endemic pathogen in potato fields. p11 is an 11-16 kDa protein encoded by the last open reading frame of PVM which contains cysteine rich proteins (CRPs) motif. CRPs have been identified as suppressors of gene silencing. In this study the p11 gene from 28 PVM isolates, including 16 new isolates from Iran, were used to determine the global genetic structure of PVM populations. Pairwise nucleotide sequence identity scores showed that global PVM CRP sequence similarity was between 69.3 and 100 %. This genetic diversity divided the 28 isolates into two main divergent phylogenetic clades. The rate of genetic diversity and non-synonymous to synonymous mutations (dN/dS) were significantly different between these two clades. Analysis showed that PVM CP is under significant negative selection pressure with the global ω value of 0.260.
authors.affiliation: Department of Plant Pathology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Tabasinejad
authors.affiliation: Department of Plant Pathology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Behrooz
authors.initials: B
authors.lastname: Jafarpour
authors.affiliation: Department of Plant Pathology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Zakiaghl
authors.affiliation: Department of Plant Protection, College of Agriculture, Shahrekord University, Shahrekord, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Siampour
authors.affiliation: Department of Plant Pathology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Rowhani
authors.affiliation: Department of Plant Pathology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Mehrvar
keywords: Cysteine rich proteins; Genetic diversity; Iran; Negative selection; Potato virus M