title: A novel technique for staining bulk grids bearing ultrathin sections.
abstract: This article proposes a technique for staining bulk grids bearing ultrathin sections with a silicone device consisting of a base plate and a depression bar. Specifically, bulk grids were loaded into the holes on the base plate in order, and then the depression bar was inserted into the slot of the plate to fix the inner edges of the grids and thereby form several separated cells to facilitate the subsequent staining and washing procedures. The results showed that the proposed technique can improve handling efficiency, safety and identification of grids during staining courses.
authors.affiliation: Department of Ophthalmology, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430070, China Department of Electron Microscope, Tianjin Medical University Basic Medical College, Tianjin 300070, China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Wu
authors.affiliation: Department of Ophthalmology, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430070, China.
authors.forename: He-Zheng
authors.initials: HZ
authors.lastname: Zhou
authors.affiliation: Department of Electron Microscope, Tianjin Medical University Basic Medical College, Tianjin 300070, China 13299994213@163.com.
authors.forename: Xiao-Xu
authors.initials: XX
authors.lastname: Zhou
keywords: grid; silicone; stain technique; ultrathin sections