title: A review of the human vs. porcine female genital tract and associated immune system in the perspective of using minipigs as a model of human genital Chlamydia infection.
abstract: Sexually transmitted diseases constitute major health issues and their prevention and treatment continue to challenge the health care systems worldwide. Animal models are essential for a deeper understanding of the diseases and the development of safe and protective vaccines. Currently a good predictive non-rodent model is needed for the study of genital chlamydia in women. The pig has become an increasingly popular model for human diseases due to its close similarities to humans. The aim of this review is to compare the porcine and human female genital tract and associated immune system in the perspective of genital Chlamydia infection. The comparison of women and sows has shown that despite some gross anatomical differences, the structures and proportion of layers undergoing cyclic alterations are very similar. Reproductive hormonal cycles are closely related, only showing a slight difference in cycle length and source of luteolysing hormone. The epithelium and functional layers of the endometrium show similar cyclic changes. The immune system in pigs is very similar to that of humans, even though pigs have a higher percentage of CD4(+)/CD8(+) double positive T cells. The genital immune system is also very similar in terms of the cyclic fluctuations in the mucosal antibody levels, but differs slightly regarding immune cell infiltration in the genital mucosa - predominantly due to the influx of neutrophils in the porcine endometrium during estrus. The vaginal flora in Göttingen Minipigs is not dominated by lactobacilli as in humans. The vaginal pH is around 7 in Göttingen Minipigs, compared to the more acidic vaginal pH around 3.5-5 in women. This review reveals important similarities between the human and porcine female reproductive tracts and proposes the pig as an advantageous supplementary model of human genital Chlamydia infection.
authors.affiliation: Section for Veterinary Reproduction and Obstetrics, Department of Large Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. emmalorenzen@sund.ku.dk.|Chlamydia Vaccine Research, Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark. emmalorenzen@sund.ku.dk.
authors.forename: Emma
authors.initials: E
authors.lastname: Lorenzen
authors.affiliation: Chlamydia Vaccine Research, Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark. frf@ssi.dk.
authors.forename: Frank
authors.initials: F
authors.lastname: Follmann
authors.affiliation: Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, Copenhagen, Denmark. grju@vet.dtu.dk.
authors.forename: Gregers
authors.initials: G
authors.lastname: Jungersen
authors.affiliation: Section for Veterinary Reproduction and Obstetrics, Department of Large Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. jager@sund.ku.dk.
authors.forename: Jørgen S
authors.initials: JS
authors.lastname: Agerholm
mesh_terms: D000818:Animals; D002689:Chlamydia / Q000502:physiology*; D002690:Chlamydia Infections / Q000276:immunology* / Q000382:microbiology; D004195:Disease Models, Animal; D005260:Female; D005836:Genitalia, Female / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D013552:Swine; D013556:Swine, Miniature