title: Effect of prenatal and early life paracetamol exposure on the level of neurotransmitters in rats--Focus on the spinal cord.
abstract: The present study has examined the influence of the prenatal and early life administration of paracetamol on the level of neurotransmitters in the spinal cord of rat pups. The effect of the drug was evaluated in 2-month old Wistar male rats exposed to paracetamol in doses of 5 (P5, n=9) or 15 mg/kg (P15, n=9) p.o. during the prenatal period and after birth until the completion of the second month of life. A parallel control group received tap water (Con, n=9). In this study we have determined the level of monoamines, their metabolites and amino acids in the spinal cord of rats using high performance liquid chromatography (HPLC) in the second month of life. The present experiment demonstrates the action of paracetamol at the molecular level associated with significant modulation of neurotransmission in the spinal cord related to dopaminergic and noradrenergic systems. Simultaneously, paracetamol administration increases the content of an aspartic and glutamic acids in the spinal cord at a critical time during development.
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1B, 02-097 Warsaw, Poland.
authors.forename: Kamilla
authors.initials: K
authors.lastname: Blecharz-Klin
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1B, 02-097 Warsaw, Poland.
authors.forename: Ilona
authors.initials: I
authors.lastname: Joniec-Maciejak
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1B, 02-097 Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Jawna
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1B, 02-097 Warsaw, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Pyrzanowska
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1B, 02-097 Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Piechal
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1B, 02-097 Warsaw, Poland.
authors.forename: Adriana
authors.initials: A
authors.lastname: Wawer
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1B, 02-097 Warsaw, Poland. Electronic address: etyszkiewicz@wum.edu.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Widy-Tyszkiewicz
mesh_terms: D000082:Acetaminophen / Q000633:toxicity*; D000367:Age Factors; D000469:Alkaline Phosphatase / Q000097:blood; D000596:Amino Acids / Q000378:metabolism; D018712:Analgesics, Non-Narcotic / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D002851:Chromatography, High Pressure Liquid; D003402:Creatine Kinase / Q000097:blood; D055664:Electrochemical Techniques; D005260:Female; D008297:Male; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000097:blood / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D000637:Transaminases / Q000097:blood
chemical_list: D000596:Amino Acids; D018712:Analgesics, Non-Narcotic; D018377:Neurotransmitter Agents; D000082:Acetaminophen; D000637:Transaminases; D003402:Creatine Kinase; D000469:Alkaline Phosphatase
keywords: Acetaminophen; Amino acids; Neurotransmission; Paracetamol; Spinal cord