title: Identification of a bioactive core sequence from human laminin and its applicability to tissue engineering.
abstract: Finding bioactive short peptides derived from proteins is a critical step to the advancement of tissue engineering and regenerative medicine, because the former maintains the functions of the latter without immunogenicity in biological systems. Here, we discovered a bioactive core nonapeptide sequence, PPFEGCIWN (residues 2678-2686; Ln2-LG3-P2-DN3), from the human laminin α2 chain, and investigated the role of this peptide in binding to transmembrane proteins to promote intracellular events leading to cell functions. This minimum bioactive sequence had neither secondary nor tertiary structures in a computational structure prediction. Nonetheless, Ln2-LG3-P2-DN3 bound to various cell types as actively as laminin in cell adhesion assays. The in vivo healing tests using rats revealed that Ln2-LG3-P2-DN3 promoted bone formation without any recognizable antigenic activity. Ln2-LG3-P2-DN3-treated titanium (Ti) discs and Ti implant surfaces caused the enhancement of bone cell functions in vitro and induced faster osseointegration in vivo, respectively. These findings established a minimum bioactive sequence within human laminin, and its potential application value for regenerative medicine, especially for bone tissue engineering.
authors.affiliation: Department of Prosthodontics, Seoul National University School of Dentistry, 101 Daehak-Ro, Jongno-Gu, Seoul 110-744, Republic of Korea. Electronic address: pros53@snu.ac.kr.
authors.forename: In-Sung
authors.initials: IS
authors.lastname: Yeo
authors.affiliation: Department of Oral and Maxillofacial Surgery, Seoul National University School of Dentistry, 101 Daehak-Ro, Jongno-Gu, Seoul 110-744, Republic of Korea. Electronic address: snowmanonmars@gmail.com.
authors.forename: Seung-Ki
authors.initials: SK
authors.lastname: Min
authors.affiliation: Department of Oral Biochemistry and Program in Cancer and Developmental Biology and Dental Research Institute, Seoul National University School of Dentistry, 101 Daehak-Ro, Jongno-Gu, Seoul 110-744, Republic of Korea. Electronic address: kang1978@snu.ac.kr.
authors.forename: Hyun Ki
authors.initials: HK
authors.lastname: Kang
authors.affiliation: Department of Dentistry, St. Vincent Hospital, Catholic University of Korea, Ji-Dong, Paldal-Ku, Suwon 442-723, Republic of Korea. Electronic address: tega95@naver.com.
authors.forename: Taek-Ka
authors.initials: TK
authors.lastname: Kwon
authors.affiliation: Department of Oral Biochemistry and Program in Cancer and Developmental Biology and Dental Research Institute, Seoul National University School of Dentistry, 101 Daehak-Ro, Jongno-Gu, Seoul 110-744, Republic of Korea. Electronic address: jsy1618@snu.ac.kr.
authors.forename: Sung Youn
authors.initials: SY
authors.lastname: Jung
authors.affiliation: Department of Oral Biochemistry and Program in Cancer and Developmental Biology and Dental Research Institute, Seoul National University School of Dentistry, 101 Daehak-Ro, Jongno-Gu, Seoul 110-744, Republic of Korea. Electronic address: bmmin@snu.ac.kr.
authors.forename: Byung-Moo
authors.initials: BM
authors.lastname: Min
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001842:Bone and Bones / Q000473:pathology; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D002470:Cell Survival; D002478:Cells, Cultured; D006801:Humans; D007797:Laminin / Q000737:chemistry*; D008297:Male; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry; D016348:Osseointegration; D010006:Osteoblasts / Q000378:metabolism; D010012:Osteogenesis; D016716:PC12 Cells; D010455:Peptides / Q000737:chemistry; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods*; D014025:Titanium / Q000737:chemistry; D055114:X-Ray Microtomography
chemical_list: D007797:Laminin; D010455:Peptides; C102645:laminin alpha 2; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: Bone formation; Laminin; Osseointegration; Osteoblast differentiation; PPFEGCIWN motif