title: Chemical Composition Analysis, Antimicrobial Activity and Cytotoxicity Screening of Moss Extracts (Moss Phytochemistry).
abstract: Mosses have been neglected as a study subject for a long time. Recent research shows that mosses contain remarkable and unique substances with high biological activity. The aim of this study, accordingly, was to analyze the composition of mosses and to screen their antimicrobial and anticancer activity. The total concentration of polyphenols and carbohydrates, the amount of dry residue and the radical scavenging activity were determined for a preliminary evaluation of the chemical composition of moss extracts. In order to analyze and identify the substances present in mosses, two types of extrahents (chloroform, ethanol) and the GC/MS and LC-TOF-MS methods were used. The antimicrobial activity was tested on four bacteria strains, and the anticancer activity on six cancer cell lines. The obtained results show the presence of a high number of primary (fatty acids and amino acids), but mainly secondary metabolites in moss extracts-including, sterols, terpenoids, polyphenols and others-and a high activity with respect to the studied test organisms.
authors.affiliation: Department of Environmental Science, University of Latvia, 19 Raina Blvd., Riga LV-1586, Latvia. laura.klavina@lu.lv.
authors.forename: Laura
authors.initials: L
authors.lastname: Klavina
authors.affiliation: Institute of Biology, University of Latvia, 3 Miera Street, Salaspils LV-2169, Latvia. gunta.springe@lu.lv.
authors.forename: Gunta
authors.initials: G
authors.lastname: Springe
authors.affiliation: Department of Microbiology and Biotechnology, University of Latvia, 4 Kronvalda Blvd., Riga LV-1010, Latvia. vizma.nikolajeva@lu.lv.
authors.forename: Vizma
authors.initials: V
authors.lastname: Nikolajeva
authors.affiliation: Faculty of Chemistry, University of Latvia, 19 Raina Blvd., Riga LV-1586, Latvia. martsinkevich.illia@gmail.com.
authors.forename: Illia
authors.initials: I
authors.lastname: Martsinkevich
authors.affiliation: Faculty of Chemistry, University of Latvia, 19 Raina Blvd., Riga LV-1586, Latvia. ilva.nakurte@lu.lv.
authors.forename: Ilva
authors.initials: I
authors.lastname: Nakurte
authors.affiliation: Faculty of Chemistry, University of Latvia, 19 Raina Blvd., Riga LV-1586, Latvia. diana.dzabijeva@lu.lv.
authors.forename: Diana
authors.initials: D
authors.lastname: Dzabijeva
authors.affiliation: Department of Environmental Science, University of Latvia, 19 Raina Blvd., Riga LV-1586, Latvia. iveta.steinberga@lu.lv.
authors.forename: Iveta
authors.initials: I
authors.lastname: Steinberga
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D017209:Apoptosis; D044002:Bryophyta / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology*; D059808:Polyphenols / Q000032:analysis / Q000494:pharmacology; D051381:Rats
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D002241:Carbohydrates; D010936:Plant Extracts; D059808:Polyphenols
keywords: GC/MS; LC-TOF-MS; amino acids; antimicrobial activity; antiradical activity; cytotoxicity; extraction; polyphenols