title: [Bone Cell Biology Assessed by Microscopic Approach. An application of two-photon microscopy for histopathological analysis of bone and cartilage].
abstract: Histopathological analysis has long been a gold standard in bio-medical research and clinical medicine. However, it is hard to quantitatively demonstrate histopathological difference. In this review, I will briefly describe principals of two-photon microscopy, and then introduce an application of second harmonic generation-based imaging for quantitative histopathological analysis of cartilage and bone.
authors.affiliation: Division of Analytical Bio-Medicine, Advanced Research Support Center (ADRES), Ehime University, Japan./Division of Bio-Imaging, Proteo-Science Center (PROS), Ehime University, Japan./Translational Research Center and Artificial Joint Integrated Center, Ehime University Hospital, Japan.
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Iimura
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000473:pathology; D001842:Bone and Bones / Q000166:cytology*; D002356:Cartilage / Q000166:cytology*; D006801:Humans; D007834:Lasers; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*