title: Novel Food Supplement "CP1" Improves Motor Deficit, Cognitive Function, and Neurodegeneration in Animal Model of Parkinson's Disease.
abstract: Based on pivotal roles of oxidative stress, dopaminergic and cholinergic systems on the pathophysiology of Parkinson's disease (PD), the searching for functional food for patients attacked with PD from Cyperus rotundus and Zingiber officinale, the substances possessing antioxidant activity, and the suppression effects on monoamine oxidase B (MAO-B) and acetylcholinesterase (AChE) have been considered. In this study, we aimed to determine the effect of the combined extract of C. rotundus and Z. officinale (CP1) to improve motor and memory deficits, neurodegeneration, oxidative stress, and functions of both cholinergic and dopaminergic systems in the animal model of PD induced by 6-hydroxydopamine hydrochloride (6-OHDA). Male Wistar rats, weighing 180-220 g, were induced unilateral lesion at right substantia nigra by 6-OHDA and were orally given CP1 at doses of 100, 200, and 300 mg/kg body weight for 14 days after 6-OHDA injection. The results showed that the 6-OHDA rats treated with CP1 increased spatial memory, but decreased neurodegeneration, malondialdehyde level, and AChE activity in hippocampus. The decreased motor disorder and neurodegeneration in substantia nigra together with the enhanced catalase activity, but decreased MAO-B activity in striatum, were also observed. The memory enhancing effect of CP1 might occur through the improved oxidative stress and the enhanced cholinergic function, whereas the effect to improve motor disorder of CP1 might occur through the enhanced dopaminergic function in striatum by decreasing the degeneration of dopaminergic neurons and the suppression of MAO-B. Therefore, CP1 is the potential functional food against PD. However, further researches in clinical trial and drug interactions are essential.
authors.affiliation: 1 Department of Physiology, Faculty of Medicine, Khon Kaen University , Khon Kaen, Thailand .|2 Integrative Complementary Alternative Medicine Research and Development Center, Khon Kaen University , Khon Kaen, Thailand .
authors.forename: Jintanaporn
authors.initials: J
authors.lastname: Wattanathorn
authors.affiliation: 2 Integrative Complementary Alternative Medicine Research and Development Center, Khon Kaen University , Khon Kaen, Thailand .|3 Neuroscience Program, Department of Physiology, Faculty of Medicine, Khon Kaen University , Khon Kaen, Thailand .
authors.forename: Chatchada
authors.initials: C
authors.lastname: Sutalangka
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001522:Behavior, Animal; D001921:Brain / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D059329:Cholinergic Neurons / Q000378:metabolism / Q000473:pathology; D003071:Cognition; D029786:Cyperus; D019587:Dietary Supplements; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000378:metabolism / Q000473:pathology; D055951:Functional Food; D058851:GPI-Linked Proteins / Q000378:metabolism; D020939:Zingiber officinale; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008995:Monoamine Oxidase / Q000378:metabolism; D009043:Motor Activity; D009410:Nerve Degeneration; D018384:Oxidative Stress; D016627:Oxidopamine; D010302:Parkinson Disease, Secondary / Q000178:diet therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D010936:Plant Extracts; D017208:Rats, Wistar; D065852:Spatial Memory; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: C000615776:CP1 plant extract; D058851:GPI-Linked Proteins; D010936:Plant Extracts; D008315:Malondialdehyde; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase; D008995:Monoamine Oxidase; D000110:Acetylcholinesterase; C548246:Ache protein, rat