title: Upregulation of microRNA-224 sensitizes human cervical cells SiHa to paclitaxel.
abstract: OBJECTIVE
  The purpose of this study was to identify the drug resistant role of miR-224 expression in cervical cancer. Materials and
  
  
  METHODS
  The expression of miR-224 pre- and post-paclitaxel treatment was determined by using stem-loop real-time reverse transcription polymerase chain reaction (RT-PCR). The authors exogenously upregulated miR-224 expression in SiHa cells using miRIDIAN miR-224 mimic transfection and observed its impact on paclitaxel sensitivity using Cytotoxicity assays.
  
  
  RESULTS
  MiR-224 was significantly downregulated with fold values at 2.130435 and 4.26087 under five and ten nM paclitaxel treatments, respectively. MiR-224 expression is markedly increased in SiHa cells after transfected with miRIDIAN miR-224 mimic. Exogenous miR-224 facilitates paclitaxel sensitivity in cervical cancer cells. The IC50 value was decreased in SiHa with overexpression of miR-224 compared with miRNA-negative control (p < 0.0001).
  
  
  CONCLUSIONS
  The results suggests that miR-224 might serve as a predictor for paclitaxel response or a therapeutic target in cervical cancer therapy.
authors.forename: F
authors.initials: F
authors.lastname: Lin
authors.forename: P
authors.initials: P
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Shen
authors.forename: X
authors.initials: X
authors.lastname: Xie
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000032:analysis / Q000502:physiology*; D017239:Paclitaxel / Q000494:pharmacology*; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000235:genetics
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C546140:MIRN224 microRNA, human; D035683:MicroRNAs; D017239:Paclitaxel