title: [Bone Cell Biology Assessed by Microscopic Approach. Novel insights about bone tissue by new microscopy systems].
abstract: The osteocytic cytoplasmic processes show regularly-arranged three-dimensional structure, a cellular network called osteocytic lacunar-canalicular system (OLCS). We have demonstrated the ultrastructure of the cellular network of OLCS by means of a structured illumination microscope method (SIM) and a Focused Ion Beam-Scanning Electron Microscope (FIB-SEM). We also attempted to localize exogenously-administered minodronate, a new generation of bisphosphonate, as well as calcium deposition onto the bone forming surface, using an isotope microscope system. Recent devised microscopic technique may provide new insights in the research field of bone.
authors.affiliation: Department of Developmental Biology of Hard Tissue, Division of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Tsuboi
authors.forename: Tomoka
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hongo
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Yurimoto
authors.forename: Sachio
authors.initials: S
authors.lastname: Kobayashi
mesh_terms: D001842:Bone and Bones / Q000166:cytology*; D006801:Humans; D021621:Imaging, Three-Dimensional; D008853:Microscopy / Q000379:methods*; D010010:Osteoclasts / Q000166:cytology; D010011:Osteocytes / Q000166:cytology