title: Effects of Glyprolines on DNA Synthesis and Free Radical Oxidation in Mouse Gastric Mucosa Under Physiological Conditions and During Therapy with Oral Non-Steroid Anti-Inflammatory Drugs.
abstract: Studies by (3)H-thymidin autoradiography showed that injections of Pro-Gly-Pro and Arg-Gly-Pro peptides caused no changes in the DNA synthesis processes in the gastric mucosa. Both peptides induced a reduction of free radical oxidation activity, which was shown by chemiluminescence. Indomethacin induced lesions in the gastric mucosa, triggered oxidative stress, and reduced proliferative activity. Injection of Pro-Gly-Pro peptide before indomethacin corrected disorders in oxidative status and normalized DNA synthesis. Preinjection of Arg-Gly-Pro led to enlargement (by 4.6 times) of the focus of lesions in animals treated by indomethacin and augmented oxidative stress.
authors.affiliation: Central Research Laboratory, Far-Eastern State Medical University, Khabarovsk, Russia. marf@yandex.ru.
authors.forename: M Yu
authors.initials: MY
authors.lastname: Fleishman
authors.affiliation: Central Research Laboratory, Far-Eastern State Medical University, Khabarovsk, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Tolstenok
authors.affiliation: Central Research Laboratory, Far-Eastern State Medical University, Khabarovsk, Russia.|Khabarovsk Branch of Far-Eastern Research Centre of Physiology and Pathology of Breathing, Siberian Division of Russian Academy of Medical Sciences - Research Institute of Mother and Child Care, Khabarovsk, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Lebed'ko
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Andreeva
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: N F
authors.initials: NF
authors.lastname: Myasoedov
authors.affiliation: Central Research Laboratory, Far-Eastern State Medical University, Khabarovsk, Russia.
authors.forename: S S
authors.initials: SS
authors.lastname: Timoshin
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use*; D004261:DNA Replication / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D005756:Gastritis / Q000139:chemically induced / Q000188:drug therapy; D007213:Indomethacin; D008297:Male; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011392:Proline / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016166:Free Radical Scavengers; D005609:Free Radicals; D009842:Oligopeptides; C000608939:arginyl-glycyl-proline; C113164:prolyl-glycyl-proline; D011392:Proline; D007213:Indomethacin
keywords: DNA synthesis; NSAID-induced gastropathy; free radical oxidation; glyprolines