title: Heterologous expression and purification of aldehyde dehydrogenase gene from Bacillus halodurans XJU-1.
abstract: BACKGROUND
  For its function of catalysing the reaction of aldehyde to acetic acid, ALDH could be applied to antialcoholismic drug development. However, both of the production and activity of natural ALDH are too low to meet the demand.
  
  
  OBJECTIVE
  To empirically explore whether aldh gene in Bacillus halodurans XJU-1 could be highly expressed in E.coli BL21(DE3) and to investigate the purification of recombinant protein ALDH.
  
  
  METHODS
  The aldh gene in Bacillus halodurans XJU-1 was cloned into prokaryotic expression vector pET30b(+), then transformed into E.coli BL21(DE3) to induce expression of ALDH. Immobilized metal ion affinity chromatography (IMAC) was applied in the separation and purification for ALDH proteins.
  
  
  RESULTS
  The recombinant vector for aldh gene was constructed and expressed in BL21(DE3) successfully. The ALDH recombinant protein was purified by ammonium sulfate precipitation and IMAC, and obtained 30.1% of the recovery with a purification factor 8.81.
  
  
  CONCLUSIONS
  The value of these findings, as well as wider implications for increasing the yield and the activity of ALDH, is meaningful.
authors.affiliation: West China School of Preclinical and Forensic Medicine, Sichuan University, Sichuan, China.|Institute of Laboratory Medicine, Beihua University, Jilin, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Wang
authors.affiliation: West China School of Preclinical and Forensic Medicine, Sichuan University, Sichuan, China.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Gao
authors.affiliation: Institute of Laboratory Medicine, Beihua University, Jilin, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Gao
authors.affiliation: West China School of Preclinical and Forensic Medicine, Sichuan University, Sichuan, China.
authors.forename: Haisheng
authors.initials: H
authors.lastname: Bao
authors.affiliation: Institute of Laboratory Medicine, Beihua University, Jilin, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chemical Engineering, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Li
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D001407:Bacillus / Q000235:genetics*; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D011994:Recombinant Proteins
chemical_list: D017931:DNA Primers; D011994:Recombinant Proteins; D000444:Aldehyde Dehydrogenase
keywords: Aldehyde  dehydrogenase (ALDH); heterologous expression; pET30b(+)-aldh vector