title: Sperm chromatin dispersion by formaldehyde in Wistar rats.
abstract: Formaldehyde (FA) is an environmental xenobiotic, which is genotoxic and carcinogenic to humans and animals; it induces DNA damage, mutations, and clastogenicity during critical cytogenetic events. FA-mediated oxidative stress is an important mechanism that has been associated with the induction of cytotoxic and genotoxic damage. Therefore, the objective of this study was to evaluate the dispersion of sperm chromatin and reproductive parameters induced by exposure to different concentrations of FA in Wistar rats. Compared to the percentage of sperm with fragmented DNA in the control group (18.10 ± 8.62%), the percentage of sperm with fragmented DNA increased following exposure to 5, 10, and 30 mg FA/kg body weight (29.60 ± 8.44, 85.20 ± 20.94 and 96.0 ± 7.87, respectively; P = 0.0001). Histopathological alterations were evident, especially in the seminiferous tubules. In conclusion, this study provides experimental evidence concerning the genotoxicity of FA, with particular reference to the decreased sperm concentration and motility and increased dispersion of DNA chromatin in rats.
authors.affiliation: Departamento de Biología Celular y Ultraestructura, Centro de Investigación Biomédica, Facultad de Medicina de Torreón, Universidad Autónoma de Coahuila, Torreón, Coahuila, México.
authors.forename: N D
authors.initials: ND
authors.lastname: Betancourt-Martínez
authors.affiliation: Departamento de Biología Celular y Ultraestructura, Centro de Investigación Biomédica, Facultad de Medicina de Torreón, Universidad Autónoma de Coahuila, Torreón, Coahuila, México.
authors.forename: J
authors.initials: J
authors.lastname: Jiménez-Villarreal
authors.affiliation: Departamento de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, Nuevo León, México.
authors.forename: P
authors.initials: P
authors.lastname: Carranza-Rosales
authors.affiliation: División de Investigación, Unidad Médica de Alta Especialidad #34, Instituto Mexicano del Seguro Social, Monterrey, Nuevo León, México.
authors.forename: N E
authors.initials: NE
authors.lastname: Guzmán-Delgado
authors.affiliation: Departamento de Ciencias Veterinarias, Universidad Autónoma Agraria Antonio Narro, Unidad Torreón, Coahuila, México.
authors.forename: C
authors.initials: C
authors.lastname: Leyva Orasma
authors.affiliation: Departamento de Biología Celular y Ultraestructura, Centro de Investigación Biomédica, Facultad de Medicina de Torreón, Universidad Autónoma de Coahuila, Torreón, Coahuila, México.
authors.forename: E
authors.initials: E
authors.lastname: Viveros Valdez
authors.forename: J
authors.initials: J
authors.lastname: Morán-Martínez
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002843:Chromatin / Q000187:drug effects* / Q000648:ultrastructure; D053938:DNA Fragmentation / Q000187:drug effects; D005557:Formaldehyde / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000187:drug effects* / Q000648:ultrastructure; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000648:ultrastructure; D013737:Testis / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D002843:Chromatin; D009153:Mutagens; D005557:Formaldehyde