title: Fangchinoline suppresses the growth and invasion of human glioblastoma cells by inhibiting the kinase activity of Akt and Akt-mediated signaling cascades.
abstract: Glioblastoma multiforme (GBM) is one of the most palindromic and malignant central nervous system neoplasms, and the current treatment is not effectual for GBM. Research of specific medicine for GBM is significant. Fangchinoline possesses a wide range of pharmacological activities and attracts more attentions due to its anti-tumor effects. In this study, two WHO grade IV human GBM cell lines (U87 MG and U118 MG) were exposed to fangchinoline, and we found that fangchinoline specifically inhibits the kinase activity of Akt and markedly suppresses the phosphorylation of Thr308 and Ser473 of Akt in human GBM cells. We also observed that fangchinoline inhibits tumor cell proliferation and invasiveness and induces apoptosis through suppressing the Akt-mediated signaling cascades, including Akt/p21, Akt/Bad, and Akt/matrix metalloproteinases (MMPs). These data demonstrated that fangchinoline exerts its anti-tumor effects in human glioblastoma cells, at least partly by inhibiting the kinase activity of Akt and suppressing Akt-mediated signaling cascades.
authors.affiliation: Institute of Neurological Medicine, General Hospital of Shenyang Military Command, 83#Wenhua Road, Shenhe District, Shenyang, Liaoning, 110016, China.
authors.forename: Bingyu
authors.initials: B
authors.lastname: Guo
authors.affiliation: Institute of Neurological Medicine, General Hospital of Shenyang Military Command, 83#Wenhua Road, Shenhe District, Shenyang, Liaoning, 110016, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
authors.affiliation: Institute of Neurological Medicine, General Hospital of Shenyang Military Command, 83#Wenhua Road, Shenhe District, Shenyang, Liaoning, 110016, China.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Su
authors.affiliation: Institute of Neurological Medicine, General Hospital of Shenyang Military Command, 83#Wenhua Road, Shenhe District, Shenyang, Liaoning, 110016, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Institute of Neurological Medicine, General Hospital of Shenyang Military Command, 83#Wenhua Road, Shenhe District, Shenyang, Liaoning, 110016, China. lxm5668sw@yahoo.com.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Neurological Medicine, General Hospital of Shenyang Military Command, 83#Wenhua Road, Shenhe District, Shenyang, Liaoning, 110016, China.
authors.forename: Guobiao
authors.initials: G
authors.lastname: Liang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D044182:Benzylisoquinolines / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000188:drug therapy / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D005909:Glioblastoma / Q000188:drug therapy* / Q000378:metabolism; D006801:Humans; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D044182:Benzylisoquinolines; C060802:fangchinoline; D051057:Proto-Oncogene Proteins c-akt
keywords: Akt; Fangchinoline; Glioblastoma multiforme; Phosphorylation; Signaling cascade