title: Immunomodulatory potential of a bioactive fraction from the leaves of Phyllostachys bambusoides (bamboo) in BALB/c mice.
abstract: In order to evaluate the role of ethyl acetate fraction (PB-EtAC) obtained from the Phyllostachys bambusoides leaves in the modulation of immune responses, detailed studies were carried out using a panel of in vivo assays. Oral administration of PB-EtAC (50-200 mg/Kg) stimulated the IgM and IgG titre expressed in the form of haemagglutination antibody (HA) titre. Further, it elicited a dose related increase in the delayed type hypersensitivity reaction (DTH) after 24 and 48 h in BALB/c mice. Besides augmenting the humoral and cell mediated immune response, the concentration of cytokines (IFN-γ, IL-2, and IL-4) in serum with respect to T cell interactions also increased significantly. It also induced macrophage phagocytosis, and nitric oxide (NO) production which resulted in a high degree of protection against Candida albicans and carbon clearance. Moreover, the enhancement in CD4 and CD8 cell populations as revealed by flow cytometry. Taken together this in vivo and ex vivo preclinical data, our results suggested that PB-EtAC acts as an effective immunostimulator eliciting both Th1 and Th2 immune responses. We are reporting first time the immunostimulatory potential of P. bambusoides and it might be regarded as a biological response modifier.
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences Shoolini University Solan (H.P.) 173229, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences Shoolini University Solan (H.P.) 173229, India.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Sharma
authors.affiliation: Division of Pharmacology, Indian Institute of Integrative Medicine (CSIR), Jammu 180001, India.
authors.forename: Tabasum
authors.initials: T
authors.lastname: Sidiq
authors.affiliation: Division of Pharmacology, Indian Institute of Integrative Medicine (CSIR), Jammu 180001, India.
authors.forename: Anamika
authors.initials: A
authors.lastname: Khajuria
authors.affiliation: Department of Pharmacognosy, School of Pharmaceutical Sciences Shoolini University Solan (H.P.) 173229, India.
authors.forename: Mahendra
authors.initials: M
authors.lastname: Jain
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences Shoolini University Solan (H.P.) 173229, India.
authors.forename: Deepak
authors.initials: D
authors.lastname: Bhagwat
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences Shoolini University Solan (H.P.) 173229, India.
authors.forename: K L
authors.initials: KL
authors.lastname: Dhar
keywords: Candida albicans; delayed type hypersensitivity reaction; flow cytometry; haemagglutination antibody titre; nitric oxide; phagocytosis