title: Multiplex biomarker analysis biosensor for detection of hepatitis B virus.
abstract: In this paper, we report the development of a protein microarray-based biosensor for the detection of the hepatitis B virus (HBV) serological markers using surface plasmon resonance (SPR Printing buffer, protein immobilization time and concentration of the capture protein were optimized systematically to determine the best performance of the biosensor. Under optimal conditions, five hepatitis B markers in 20 μL human serum can be simultaneously detected within 30 minutes, whereas other methods such as ELISA and PCR can detect only one marker within four hours. This platform has been validated by analysis of 35 patients known to have hepatitis B, with 85% agreement between the test platform and analysis by commercial enzyme-linked immunosorbent assay (ELISA) kits. The results demonstrate that the protein microarray with SPR displayed a sensitivity of 0.1 ng mL(-1) for HBsAg. In addition to high sensitivity, it also shows excellent specificity, reproducibility and stability. This integrated protein microarray technique combined with SPR is a promising candidate for hepatitis B diagnosis with high-throughput.
authors.affiliation: Institute of International Travel Health Care, Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518033, P.R. China.|Shenzhen Children's Hospital, Shenzhen, 518038, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.affiliation: Institute of International Travel Health Care, Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518033, P.R. China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Gu
authors.affiliation: Institute of International Travel Health Care, Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518033, P.R. China.
authors.forename: Jian'an
authors.initials: J
authors.lastname: He
authors.affiliation: Institute of International Travel Health Care, Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518033, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: Guangdong Medical college, Zhanjiang, 524023, P.R. China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Yao
authors.affiliation: Institute of International Travel Health Care, Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518033, P.R. China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of International Travel Health Care, Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518033, P.R. China.
authors.forename: Chunzhong
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Institute of International Travel Health Care, Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518033, P.R. China.
authors.forename: Yunqing
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of public health, Nantong University, Nantong, 226019, P.R. China.
authors.forename: Shengyang
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Medical Inspection center, Zhujiang Hospital, Nan Fang Medical University, Guangzhou, 510280, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Long
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006509:Hepatitis B / Q000097:blood* / Q000175:diagnosis*; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D040081:Protein Array Analysis; D015203:Reproducibility of Results; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D055910:Antibodies, Immobilized; D015415:Biomarkers; D006514:Hepatitis B Surface Antigens
keywords: Surface plasmon resonance; biosensor; hepatitis B virus; label-free; protein microarray