title: Effects of polyvalent immunoglobulins in patients with recurrent pregnancy loss and antibodies to the choriocarcinoma cell line JEG-3.
abstract: OBJECTIVE
  The benefit of polyvalent immunoglobulins (IVIG) for patients with recurrent pregnancy loss (RPL) is controversially discussed. Anti-trophoblast antibodies as an expression of immune pathology have been associated with RPL. We investigated whether the antibody activity against the choriocarcinoma cell line JEG-3 in RPL patients is influenced in vitro by IVIG.
  
  
  METHODS
  Sera of 110 unexplained RPL patients with positive anti-JEG-3 antibodies were coincubated with IVIG in different concentrations (10mg/ml, 20mg/ml, 40mg/ml). Coincubation with human albumin in identical concentrations served as control. Anti-JEG-3 reactivity was measured by using flow cytometry in comparisons with two in-house standards antibody probes of low and high reactivity as described before. Anti-JEG-3 reactivity above the 95% confidence interval of controls was defined as positive.
  
  
  RESULTS
  Incubating RPL sera with 10mg/ml IVIG significantly decreased anti-JEG-3 activity (p<0.001). Increasing IVIG concentration to 40mg/ml resulted in a slightly additionally reduction (p=0.42). In contrast, coincubation with albumin in identically concentrations did not affect anti-JEG-3 activity (p>0.40).
  
  
  CONCLUSIONS
  Coincubation with IVIG in vitro leads to a significant suppression of anti-JEG-3 activity in the sera of RPL patients.
authors.affiliation: Division of Gynecological Endocrinology and Reproductive Medicine, Department of Gynecology and Obstetrics, Ludwig-Maximilians-University, Marchioninistrasse 15, 81377 Munich, Germany. Electronic address: nina.rogenhofer@med.uni-muenchen.de.
authors.forename: Nina
authors.initials: N
authors.lastname: Rogenhofer
authors.affiliation: Division of Gynecological Endocrinology and Reproductive Medicine, Department of Gynecology and Obstetrics, Ludwig-Maximilians-University, Marchioninistrasse 15, 81377 Munich, Germany.
authors.forename: Viktoria
authors.initials: V
authors.lastname: von Schönfeldt
authors.affiliation: Fertility Center Augsburg, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Ochsenkühn
authors.affiliation: Division of Gynecological Endocrinology and Reproductive Medicine, Department of Gynecology and Obstetrics, Ludwig-Maximilians-University, Marchioninistrasse 15, 81377 Munich, Germany.
authors.forename: Felicia
authors.initials: F
authors.lastname: Sili
authors.affiliation: Division of Gynecological Endocrinology and Reproductive Medicine, Department of Gynecology and Obstetrics, Ludwig-Maximilians-University, Marchioninistrasse 15, 81377 Munich, Germany.
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Thaler
mesh_terms: D000026:Abortion, Habitual / Q000276:immunology*; D000328:Adult; D000418:Albumins / Q000494:pharmacology; D000912:Antibodies, Neoplasm / Q000097:blood / Q000187:drug effects*; D045744:Cell Line, Tumor; D002822:Choriocarcinoma / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D016756:Immunoglobulins, Intravenous / Q000494:pharmacology*; D007155:Immunologic Factors / Q000494:pharmacology*; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000276:immunology*; D055815:Young Adult
chemical_list: D000418:Albumins; D000912:Antibodies, Neoplasm; D007106:Immune Sera; D016756:Immunoglobulins, Intravenous; D007155:Immunologic Factors
keywords: Immunoglobulins; JEG-3; Pregnancy loss; Recurrent miscarriages; Trophoblast antibodies