title: Facile synthesis of nano-sized agarose based amino acid-Its pH-dependent protein-like behavior and interactions with bovine serum albumin.
abstract: In a facile synthesis agarose was amphoterically functionalized to afford nano-sized agarose amino acids, aminoagarose succinate half-esters (AAE) containing one pendant carboxyl group. Nano-sized AAEs (<10 nm; DLS) were characterized and they had three various degrees of substitution [overall DSs 0.88, 0.89 and 0.96], both the amino and half-ester groups were placed on C-6 positions of the 1,3 beta-d-galactopyranose moieties of agarose backbone ((13)C NMR). AAEs performed like large protein molecules exhibiting pH-responsive structural variations (optical rotatory dispersion), presenting a mixed solubility pattern like random coil (soluble) and aggregate (precipitation) formations. Circular dichroism studies showed their pH-dependent associative interactions with bovine serum albumin, which indicated complexation at acidic and basic pHs, and decomplexation at pH 6.8 with AAE (DS 0.96). Thus, these nano-sized AAE based systems may be of potential utility in the domains demanding the merits of preferential protein bindings e.g. pH-responsive cationic/anionic drug carrier, separations or chiral sensing applications.
authors.affiliation: Marine Biotechnology and Ecology Division, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar 364002, Gujarat, India.
authors.forename: Nishith A
authors.initials: NA
authors.lastname: Chudasama
authors.affiliation: Marine Biotechnology and Ecology Division, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar 364002, Gujarat, India; Academy of Scientific and Innovative Research, Anusandhan Bhavan, 2 Rafi Marg, New Delhi 110001, India. Electronic address: aks@csmcri.org.
authors.forename: A K
authors.initials: AK
authors.lastname: Siddhanta
mesh_terms: D000596:Amino Acids / Q000138:chemical synthesis*; D000606:Amino Sugars / Q000138:chemical synthesis*; D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D002942:Circular Dichroism; D004952:Esters; D005427:Flocculation; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D049329:Nanostructures / Q000737:chemistry*; D057786:Peptidomimetics / Q000138:chemical synthesis*; D011485:Protein Binding; D012685:Sepharose / Q000138:chemical synthesis*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D013386:Succinates / Q000737:chemistry
chemical_list: D000596:Amino Acids; D000606:Amino Sugars; D004952:Esters; D057786:Peptidomimetics; D013386:Succinates; D012710:Serum Albumin, Bovine; D012685:Sepharose
keywords: Amino acids; Amphoteric agarose; BSA binding; Nano-sized proteins; Polysaccharides