title: Immunoproteomic technology offers an extraordinary diagnostic approach for Toxoplasma gondii infection.
abstract: Immunoproteomic technology offers an exceptional tool to fill the blanks that still exist in diagnosis of Toxoplasma gondii infection despite its annotated sequence. The pitfalls of serological assays and current immunoproteomic approaches are accentuated, and new approaches are presented to improve the signal and to eliminate the noise produced by blocking-specific background. This review also highlights examples where immunoproteomic studies have contributed to broaden our understanding of toxoplasmosis diagnosis. Further promising solutions, which immunoproteomic technology can grant for toxoplasmosis diagnosis are part of an intense discussion.
authors.affiliation: National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China; Faculty of Science, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt. Electronic address: saeed_elashram@yahoo.com.
authors.forename: Saeed
authors.initials: S
authors.lastname: El-Ashram
authors.affiliation: National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yin
authors.affiliation: University of Guelph, Guelph, Ontario, Canada.
authors.forename: John R
authors.initials: JR
authors.lastname: Barta
authors.affiliation: National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Jamal
authors.initials: J
authors.lastname: Khan
authors.affiliation: National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Liu
authors.affiliation: National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, Beijing 100193, China. Electronic address: suoxun@cau.edu.cn.
authors.forename: Xun
authors.initials: X
authors.lastname: Suo
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D040901:Proteomics / Q000379:methods*; D015800:Protozoan Proteins / Q000737:chemistry; D014122:Toxoplasma / Q000737:chemistry* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D014123:Toxoplasmosis / Q000175:diagnosis / Q000469:parasitology*
chemical_list: D015800:Protozoan Proteins
keywords: Diagnosis; Immunoproteomic technology; Toxoplasmosis