title: Inhibition of migration and induction of apoptosis in LoVo human colon cancer cells by polysaccharides from Ganoderma lucidum.
abstract: Ganoderma lucidum polysaccharides (GLPs), which were purified from the medicinal herb G. lucidum followed by ethanol precipitation, protein depletion using the Sevage assay, purification using DEAE‑cellulose (DE-52), dialysis and the use of ultrafiltration membranes, are used as an ingredient in traditional anticancer treatments in China. The aim of the current study was to evaluate the anticancer effects and investigate the underlying molecular mechanisms of GLPs on LoVo human colon cancer cells. The results demonstrated that the GLP‑mediated anticancer effect in LoVo cells was characterized by cytotoxicity, migration inhibition, enhanced DNA fragmentation, morphological alterations and increased lactate dehydrogenase release. Furthermore, the activation of caspases‑3, ‑8 and ‑9 was involved in GLP‑stimulated apoptosis. Additionally, treatment with GLPs promoted the expression of Fas and caspase‑3 proteins, whilst reducing the expression of cleaved poly(ADP‑ribose) polymerase. These data indicate that GLPs demonstrate potential antitumor activity in human colon cancer cells, predominantly through the inhibition of migration and induction of apoptosis. Furthermore, activation of the Fas/caspase-dependent apoptosis pathway is involved in the cytotoxicity of GLPs.
authors.affiliation: Hunan Agricultural Product Processing Institute, Changsha, Hunan 410128, P.R. China.
authors.forename: Zeng-Enni
authors.initials: ZE
authors.lastname: Liang
authors.affiliation: College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, P.R. China.
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Yi
authors.affiliation: State Key Laboratory of Sub‑Health Intervention Technology, State Administration of Traditional Chinese Medicine, Changsha, Hunan 410128, P.R. China.
authors.forename: Yu-Tong
authors.initials: YT
authors.lastname: Guo
authors.affiliation: College of Horticulture and Landscape, Hunan Agricultural University, Changsha, Hunan 410128, P.R. China.
authors.forename: Ren-Cai
authors.initials: RC
authors.lastname: Wang
authors.affiliation: College of Horticulture and Landscape, Hunan Agricultural University, Changsha, Hunan 410128, P.R. China.
authors.forename: Qiu-Long
authors.initials: QL
authors.lastname: Hu
authors.affiliation: College of Horticulture and Landscape, Hunan Agricultural University, Changsha, Hunan 410128, P.R. China.
authors.forename: Xing-Yao
authors.initials: XY
authors.lastname: Xiong
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D020940:Reishi / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D011065:Poly(ADP-ribose) Polymerases; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9