title: Biocompatible ZnS:Mn(2+) quantum dots/SiO2 nanocomposites as fluorescent probe for imaging HeLa cell.
abstract: ZnS:Mn(2+) quantum dots (QDs) were successfully embedded in SiO2 spheres by a reverse microemulsion method. The results showed that the monodispersed core/shell nanocomposites were uniform in size, with the majority of the SiO2 nanoparticles containing one QD in the center of the sphere. The shell thickness of SiO2 increased from 7 to 18 nm as the hydrolysis time of TEOS increased from 20 to 40 h. The quantum yield (QY) of the yellow-orange emission (coming from the Mn(2+) ions (4)T1-(6)A1 transition) for the ZnS:Mn(2+)(3 %) QDs and ZnS:Mn(2+)(3 %) QDs@SiO2 (when t = 40 h) nanocomposites was measured to be 34.5 and 22.4 %, respectively. All samples showed no significant cytotoxicity against the HeLa cells even at a high concentration of 500 μg/ml after incubation for 24 h. The red fluorescence can be observed in the cytoplasm of the HeLa cell, further proving its biolabeling applications.
authors.affiliation: Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Siping, 136000, People's Republic of China. caojian_928@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
authors.affiliation: Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Siping, 136000, People's Republic of China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Niu
authors.affiliation: Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Siping, 136000, People's Republic of China.
authors.forename: Donglai
authors.initials: D
authors.lastname: Han
authors.affiliation: Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Siping, 136000, People's Republic of China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Yang
authors.affiliation: Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Siping, 136000, People's Republic of China.
authors.forename: Qianyu
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Siping, 136000, People's Republic of China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Siping, 136000, People's Republic of China. jhyang1@jlnu.edu.cn.
authors.forename: Jinghai
authors.initials: J
authors.lastname: Yang
mesh_terms: D001672:Biocompatible Materials; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008345:Manganese / Q000737:chemistry*; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures; D045663:Quantum Dots; D012822:Silicon Dioxide / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D014961:X-Ray Diffraction; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D013440:Sulfides; D017967:Zinc Compounds; D008345:Manganese; D012822:Silicon Dioxide; C031238:zinc sulfide