title: Comparative genome analysis of Lysinibacillus B1-CDA, a bacterium that accumulates arsenics.
abstract: Previously, we reported an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, isolated from an arsenic contaminated lands. Here, we have investigated its genetic composition and evolutionary history by using massively parallel sequencing and comparative analysis with other known Lysinibacillus genomes. Assembly of the sequencing reads revealed a genome of ~4.5 Mb in size encompassing ~80% of the chromosomal DNA. We found that the set of ordered contigs contains abundant regions of similarity with other Lysinibacillus genomes and clearly identifiable genome rearrangements. Furthermore, all genes of B1-CDA that were predicted be involved in its resistance to arsenic and/or other heavy metals were annotated. The presence of arsenic responsive genes was verified by PCR in vitro conditions. The findings of this study highlight the significance of this bacterium in removing arsenics and other toxic metals from the contaminated sources. The genetic mechanisms of the isolate could be used to cope with arsenic toxicity.
authors.affiliation: Systems Biology Research Center, School of Bioscience, University of Skövde, P.O. Box 408, SE-541 28 Skövde, Sweden; The Life Science Center, School of Science and Technology, Örebro University, SE-701 82 Örebro, Sweden.
authors.forename: Aminur
authors.initials: A
authors.lastname: Rahman
authors.affiliation: Systems Biology Research Center, School of Bioscience, University of Skövde, P.O. Box 408, SE-541 28 Skövde, Sweden.
authors.forename: Noor
authors.initials: N
authors.lastname: Nahar
authors.affiliation: Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Tathawade, Pune, 411033, India.
authors.forename: Neelu N
authors.initials: NN
authors.lastname: Nawani
authors.affiliation: The Life Science Center, School of Science and Technology, Örebro University, SE-701 82 Örebro, Sweden.
authors.forename: Jana
authors.initials: J
authors.lastname: Jass
authors.affiliation: School of Arts and Science, Iona College, New Rochelle, NY 10801, USA.
authors.forename: Sibdas
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Systems Biology Research Center, School of Bioscience, University of Skövde, P.O. Box 408, SE-541 28 Skövde, Sweden.
authors.forename: Björn
authors.initials: B
authors.lastname: Olsson
authors.affiliation: Systems Biology Research Center, School of Bioscience, University of Skövde, P.O. Box 408, SE-541 28 Skövde, Sweden. Electronic address: abul.mandal@his.se.
authors.forename: Abul
authors.initials: A
authors.lastname: Mandal
mesh_terms: D001151:Arsenic / Q000378:metabolism* / Q000494:pharmacology; D001406:Bacillaceae / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D014644:Genetic Variation; D016680:Genome, Bacterial / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D001151:Arsenic
keywords: Bioremediation; Gene prediction; Genome sequencing; Lysinibacillus sphaericus B1-CDA; Toxic metals; de novo assembly