title: Migration Speed of Cajal-Retzius Cells Modulated by Vesicular Trafficking Controls the Size of Higher-Order Cortical Areas.
abstract: In the neocortex, higher-order areas are essential to integrate sensory-motor information and have expanded in size during evolution. How higher-order areas are specified, however, remains largely unknown. Here, we show that the migration and distribution of early-born neurons, the Cajal-Retzius cells (CRs), controls the size of higher-order areas in the mouse somatosensory, auditory, and visual cortex. Using live imaging, genetics, and in silico modeling, we show that subtype-specific differences in the onset, speed, and directionality of CR migration determine their differential invasion of the developing cortical surface. CR migration speed is cell autonomously modulated by vesicle-associated membrane protein 3 (VAMP3), a classically non-neuronal mediator of endosomal recycling. Increasing CR migration speed alters their distribution in the developing cerebral cortex and leads to an expansion of postnatal higher-order areas and congruent rewiring of thalamo-cortical input. Our findings thus identify novel roles for neuronal migration and VAMP3-dependent vesicular trafficking in cortical wiring.
authors.affiliation: Institut Jacques Monod, CNRS UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris Cedex, France.
authors.forename: Melissa
authors.initials: M
authors.lastname: Barber
authors.affiliation: Institut Jacques Monod, CNRS UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris Cedex, France.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Arai
authors.affiliation: RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Morishita
authors.affiliation: Institut Jacques Monod, CNRS UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris Cedex, France.
authors.forename: Lisa
authors.initials: L
authors.lastname: Vigier
authors.affiliation: Institut Jacques Monod, CNRS UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris Cedex, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Causeret
authors.affiliation: Stem-cell and Brain Research Institute, INSERM U846, 69675 Bron and University of Lyon I, 69003 Lyon, France.
authors.forename: Ugo
authors.initials: U
authors.lastname: Borello
authors.affiliation: Institut Jacques Monod, CNRS UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris Cedex, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Ledonne
authors.affiliation: Institut Jacques Monod, CNRS UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris Cedex, France.
authors.forename: Eva
authors.initials: E
authors.lastname: Coppola
authors.affiliation: Institut Jacques Monod, CNRS UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris Cedex, France; ImagoSeine, Institut Jacques Monod, CNRS UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris Cedex, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Contremoulins
authors.affiliation: CNRS UPR 3212, University of Strasbourg, Institute of Cellular and Integrative Neurosciences (INCI), 67084 Strasbourg, France.
authors.forename: Frank W
authors.initials: FW
authors.lastname: Pfrieger
authors.affiliation: University of Louvain Medical School, Institute of Neuroscience, 1200 Brussels, Belgium.
authors.forename: Fadel
authors.initials: F
authors.lastname: Tissir
authors.affiliation: Department of Basic Neurosciences, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.
authors.forename: Subashika
authors.initials: S
authors.lastname: Govindan
authors.affiliation: Department of Basic Neurosciences, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.
authors.forename: Denis
authors.initials: D
authors.lastname: Jabaudon
authors.affiliation: Institut Jacques Monod, CNRS UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris Cedex, France; Université Paris Diderot, Sorbonne Paris Cité, INSERM ERL U950, "Membrane Traffic in Health and Disease," 75013 Paris, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Proux-Gillardeaux
authors.affiliation: Institut Jacques Monod, CNRS UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris Cedex, France; Université Paris Diderot, Sorbonne Paris Cité, INSERM ERL U950, "Membrane Traffic in Health and Disease," 75013 Paris, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Galli
authors.affiliation: Institut Jacques Monod, CNRS UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris Cedex, France. Electronic address: alessandra.pierani@ijm.fr.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pierani
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology*; D056885:Interstitial Cells of Cajal / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D019579:Neocortex / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D009474:Neurons / Q000378:metabolism*; D051040:Vesicle-Associated Membrane Protein 3 / Q000378:metabolism
chemical_list: D051040:Vesicle-Associated Membrane Protein 3; C494953:vesicle-associated membrane protein 3, mouse