title: Analysis of the Effect of β-Endorphin-Like Peptide Pentarphin on Intracellular Ca²⁺ Concentration in Early Mouse Embryos Using Fluorescent Probes.
abstract: At the early stages of mammalian embryo development, Ca(2+) influences the processes of cleavage and differentiation. An important role in these processes is played by peptide growth factors. One of them, pentarphin, is a peptide growth factor similar to the central part of β-endorphin molecule and a fragment 369-373 of IgG heavy chain. We used fluorescent Ca(2+) indicators Fluo-3 AM and Fura Red AM. Pentarphin added to embryos in a concentration of 0.1 μM 1.5-2 fold increased intercellular Ca(2+) level in comparison with the initial level.
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Moscow, Russia. c.h.e.r.n.o.v@rambler.ru.
authors.forename: A S
authors.initials: AS
authors.lastname: Chernov
authors.affiliation: Institute of Cell Biophysics, Pushchino, Moscow Region, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Sergievich
authors.affiliation: Institute of Cell Biophysics, Pushchino, Moscow Region, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Karnaukhova
mesh_terms: D000701:Analgesics, Opioid / Q000494:pharmacology*; D000814:Aniline Compounds / Q000737:chemistry; D000818:Animals; D020013:Calcium Signaling; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology*; D014966:Xanthenes / Q000737:chemistry
chemical_list: D000701:Analgesics, Opioid; D000814:Aniline Compounds; D005456:Fluorescent Dyes; D009842:Oligopeptides; D014966:Xanthenes; C474114:valyl-lysyl-glycyl-phenylalanyl-tyrosine; C059715:Fluo-3
keywords: fluorescent probes; intercellular Ca 2+; pentarphin; preimplantation embryos