title: Production of 2,3-butanediol by Klebsiella oxytoca from various sugars in microalgal hydrolysate.
abstract: A new fermentation process using a mixed sugar medium is proposed in this study for 2,3-butanediol (2,3-BDO) production. The medium contained seven different monosugars known to be present in Nannochloropsis oceanica hydrolysate. The performance of each sugar when existing alone or together with glucose was evaluated. All the sugars except fucose were successfully metabolized for 2,3-BDO production. A 2,3-BDO yield of 0.31g/g was achieved with the mixed sugar medium, which was very close to that with the glucose-only medium. However, the 2,3-BDO productivity (0.28 g L(-1) h(-1) ) was found to be about 30% lower than that with glucose, implying, as expected, the existence of glucose repression on the uptake of other sugars. Strain development is in need to remove such negative effect of glucose for improved process efficiency. Fucose with the lowest uptake rate and no contribution to 2,3-BDO production can be a high value-added byproduct, once recovered and purified.
authors.affiliation: Dept. of Chemical and Biomolecular Engineering (BK Plus Program), Korea Advanced Institute of Science and Technology, 291, Daehak-Ro, Yuseong-Gu, Daejeon, 305-701, Republic of Korea.
authors.forename: Yong Jae
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Dept. of Chemical and Biomolecular Engineering (BK Plus Program), Korea Advanced Institute of Science and Technology, 291, Daehak-Ro, Yuseong-Gu, Daejeon, 305-701, Republic of Korea.
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Joo
authors.affiliation: Dept. of Chemical and Biomolecular Engineering (BK Plus Program), Korea Advanced Institute of Science and Technology, 291, Daehak-Ro, Yuseong-Gu, Daejeon, 305-701, Republic of Korea.
authors.forename: Juyi
authors.initials: J
authors.lastname: Park
authors.affiliation: Research and Development Center, GS Caltex Corporation, 104-4 Munji-Dong, Yuseong-Gu, Daejeon, 305-380, Republic of Korea.
authors.forename: Duk-Ki
authors.initials: DK
authors.lastname: Kim
authors.affiliation: Dept. of Chemical and Biomolecular Engineering (BK Plus Program), Korea Advanced Institute of Science and Technology, 291, Daehak-Ro, Yuseong-Gu, Daejeon, 305-701, Republic of Korea.|KI for the Biocentury, Korea Advanced Institute of Science and Technology, 291, Daehak-Ro, Yuseong-Gu, Daejeon, 305-701, Republic of Korea.
authors.forename: Ki Jun
authors.initials: KJ
authors.lastname: Jeong
authors.affiliation: Dept. of Chemical and Biomolecular Engineering (BK Plus Program), Korea Advanced Institute of Science and Technology, 291, Daehak-Ro, Yuseong-Gu, Daejeon, 305-701, Republic of Korea.|Research and Development Center, GS Caltex Corporation, 104-4 Munji-Dong, Yuseong-Gu, Daejeon, 305-380, Republic of Korea.|KI for the Biocentury, Korea Advanced Institute of Science and Technology, 291, Daehak-Ro, Yuseong-Gu, Daejeon, 305-701, Republic of Korea.
authors.forename: Yong Keun
authors.initials: YK
authors.lastname: Chang
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D002072:Butylene Glycols / Q000032:analysis / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D041121:Klebsiella oxytoca / Q000378:metabolism*; D058086:Microalgae
chemical_list: D002072:Butylene Glycols; C026978:2,3-butylene glycol; D005947:Glucose
keywords: 2,3-butanediol; Klebsiella oxytoca; microalgae hydrolysate; sugar mixture