title: Measurement of brachial artery endothelial function using a standard blood pressure cuff.
abstract: The integrity of endothelial function in major arteries (EFMA) is a powerful independent predictor of heart attack and stroke. Existing ultrasound-based non-invasive assessment methods are technically challenging and suitable only for laboratory settings. EFMA, like blood pressure (BP), is both acutely and chronically affected by factors such as lifestyle and medication. Consequently, laboratory-based measurements cannot fully gauge the effects of medical interventions on EFMA. EFMA and BP have, arguably, comparable (but complementary) value in the assessment of cardiovascular health. Widespread deployment of EFMA assessment is thus a desirable clinical goal. To this end, we propose a device based on modifying the measurement protocol of a standard electronic sphygmomanometer. The protocol involves inflating the cuff to sub-diastolic levels to enable recording of the pulse waveform before and after vasodilatory stimulus. The mechanical unloading of the arterial wall provided by the cuff amplifies the distension that occurs with each pulse, which is measured as a pressure variation in the cuff. We show that the height of the rising edge of each pulse is proportional to the change in lumen area between diastole and systole. This allows the effect of vasodilatory stimuli on the artery to be measured with high sensitivity. We compare the proposed cuff flow-mediated dilation (cFMD) method to ultrasound flow-mediated dilation (uFMD). We find significant correlation (r = 0.55, p = 0.003, N = 27) between cFMD- and uFMD-based metrics obtained when the release of a 5 min cuff occlusion is employed to induce endothelial stimulus via reactive hyperemia. cFMD is approximately proportional to the square of uFMD, representing a typical increase in sensitivity to vasodilation of 300-600%. This study illustrates the potential for an individual to conveniently measure his/her EFMA by using a low-cost reprogrammed home sphygmomanometer.
authors.affiliation: Department of Structural Biology and Imaging, Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Maltz
authors.forename: Geoffrey H
authors.initials: GH
authors.lastname: Tison
authors.forename: Hugh F
authors.initials: HF
authors.lastname: Alley
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Budinger
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Owens
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Olgin
mesh_terms: D000328:Adult; D001795:Blood Pressure Determination / Q000592:standards; D001916:Brachial Artery / Q000502:physiology*; D004730:Endothelium, Vascular / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012015:Reference Standards; D012815:Signal Processing, Computer-Assisted; D013997:Time Factors; D000069453:Ultrasonic Waves; D014664:Vasodilation