title: Changes in the levels, expression, and possible roles of serotonin and dopamine during embryonic development in the giant freshwater prawn, Macrobrachium rosenbergii.
abstract: We investigated the changes in the levels of serotonin (5-HT) and dopamine (DA), and their possible roles during embryonic development of the freshwater prawn, Macrobrachium rosenbergii. The 5-HT and DA concentrations were quantified using high performance liquid chromatography with electrochemical detection (HPLC-ECD). The levels of 5-HT and DA gradually increased from early developing embryos to late developing embryos. The 5-HT concentrations gradually increased from the pale yellow egg to orange egg stages, and reaching a maximum at the black egg stage. DA concentrations were much lower in the early embryos than those of 5-HT (P<0.05), and gradually increased to reach the highest level at the black egg stage. Immunohistochemically, 5-HT was firstly detected in the early embryonic stages, whereas DA developed later than 5-HT. Functionally, 5-HT-treated female prawns at doses of 2.5×10(-5), 2.5×10(-6) and 2.5×10(-7)mol/prawn, produced embryos with significantly shortened lengths of early embryonic stages, whereas DA-treated prawns at all three doses, exerted its effects by significantly lengthening the period of mid-embryonic stage onwards. These results suggest significant involvement of 5-HT and DA in embryonic developmental processes of this species.
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Mahidol University, Nakhonsawan Campus, Nakhonsawan 60130, Thailand. Electronic address: anatch2002@yahoo.com.
authors.forename: Yotsawan
authors.initials: Y
authors.lastname: Tinikul
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Mahidol University, Nakhonsawan Campus, Nakhonsawan 60130, Thailand.
authors.forename: Jaruwan
authors.initials: J
authors.lastname: Poljaroen
authors.affiliation: Mahidol University, Nakhonsawan Campus, Nakhonsawan 60130, Thailand.
authors.forename: Ruchanok
authors.initials: R
authors.lastname: Tinikul
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Prasert
authors.initials: P
authors.lastname: Sobhon
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D033541:Palaemonidae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012701:Serotonin / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D012701:Serotonin; D004298:Dopamine
keywords: Dopamine; Embryonic development; Freshwater prawn; Macrobrachium rosenbergii; Neurotransmitter; Serotonin