title: Proposition of a Silica Nanoparticle-Enhanced Hybrid Spin-Microcantilever Sensor Using Nonlinear Optics for Detection of DNA in Liquid.
abstract: We theoretically propose a method based on the combination of a nonlinear optical mass sensor using a hybrid spin-microcantilever and the nanoparticle-enhanced technique, to detect and monitor DNA mutations. The technique theoretically allows the mass of external particles (ssDNA) landing on the surface of a hybrid spin-microcantilever to be detected directly and accurately at 300 K with a mass responsivity 0.137 Hz/ag in situ in liquid. Moreover, combined with the nanoparticle-enhanced technique, even only one base pair mutation in the target DNA sequence can be identified in real time accurately, and the DNA hybridization reactions can be monitored quantitatively. Furthermore, in situ detection in liquid and measurement of the proposed nonlinear optical spin resonance spectra will minimize the experimental errors.
authors.affiliation: Key Laboratory of Artificial Structures and Quantum Control (Ministry of Education), Department of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai 200240, China. hao0792@sina.cn.
authors.forename: Wen-Hao
authors.initials: WH
authors.lastname: Wu
authors.affiliation: Key Laboratory of Artificial Structures and Quantum Control (Ministry of Education), Department of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai 200240, China. zhukadi@sjtu.edu.cn.
authors.forename: Ka-Di
authors.initials: KD
authors.lastname: Zhu
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D004867:Equipment Design; D008962:Models, Theoretical; D053758:Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods; D012822:Silicon Dioxide / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry
chemical_list: D012996:Solutions; D012822:Silicon Dioxide; D004247:DNA
keywords: detection of DNA; hybrid spin sensor; nonlinear optics