title: Molecular and Biochemical Characterization of a Novel Xylanase from Massilia sp. RBM26 Isolated from the Feces of Rhinopithecus bieti.
abstract: Xylanases sourced from different bacteria have significantly different enzymatic properties. Therefore, studying xylanases from different bacteria is important to their applications in different fields. A potential xylanase degradation gene in Massilia was recently discovered through genomic sequencing. However, its xylanase activity remains unexplored. This paper is the first to report a xylanase (XynRBM26) belonging to the glycosyl hydrolase family (GH10) from the genus Massilia. The gene encodes a 383-residue polypeptide (XynRBM26) with the highest identity of 62% with the endoxylanase from uncultured bacterium BLR13. The XynRBM26 expressed in Escherichia coli BL21 is a monomer with a molecular mass of 45.0 kDa. According to enzymatic characteristic analysis, pH 5.5 is the most appropriate for XynRBM26, which could maintain more than 90% activity between pH 5.0 and 8.0. Moreover, XynRBM26 is stable at 37°C and could maintain at least 96% activity after being placed at 37°C for 1 h. This paper is the first to report that GH10 xylanase in an animal gastrointestinal tract (GIT) has salt tolerance, which could maintain 86% activity in 5 M NaCl. Under the optimum conditions, Km, Vmax, and kcat of XynRBM26 to beechwood xylan are 9.49 mg/ml, 65.79 μmol/min/mg, and 47.34 /sec, respectively. Considering that XynRBM26 comes from an animal GIT, this xylanase has potential application in feedstuff. Moreover, XynRBM26 is applicable to high-salt food and seafood processing, as well as other high-salt environmental biotechnological fields, because of its high catalytic activity in high-concentration NaCl.
authors.affiliation: School of Life Science, Yunnan Normal University, Kunming 650500, P.R. China.|Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650500, P.R. China.|Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Kunming 650500, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.affiliation: School of Life Science, Yunnan Normal University, Kunming 650500, P.R. China.
authors.forename: Liming
authors.initials: L
authors.lastname: Dai
authors.affiliation: School of Life Science, Yunnan Normal University, Kunming 650500, P.R. China.|Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650500, P.R. China.|Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Kunming 650500, P.R. China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Life Science, Yunnan Normal University, Kunming 650500, P.R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Deng
authors.affiliation: School of Life Science, Yunnan Normal University, Kunming 650500, P.R. China.
authors.forename: Huabiao
authors.initials: H
authors.lastname: Miao
authors.affiliation: School of Life Science, Yunnan Normal University, Kunming 650500, P.R. China.|Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650500, P.R. China.|Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Kunming 650500, P.R. China.
authors.forename: Junpei
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Life Science, Yunnan Normal University, Kunming 650500, P.R. China.|Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650500, P.R. China.|Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Kunming 650500, P.R. China.
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Mu
authors.affiliation: School of Life Science, Yunnan Normal University, Kunming 650500, P.R. China.|Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650500, P.R. China.|Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Kunming 650500, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.affiliation: School of Life Science, Yunnan Normal University, Kunming 650500, P.R. China.|Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650500, P.R. China.|Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Kunming 650500, P.R. China.
authors.forename: Xianghua
authors.initials: X
authors.lastname: Tang
authors.affiliation: School of Life Science, Yunnan Normal University, Kunming 650500, P.R. China.|Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650500, P.R. China.|Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Kunming 650500, P.R. China.
authors.forename: Yunjuan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: School of Life Science, Yunnan Normal University, Kunming 650500, P.R. China.|Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650500, P.R. China.|Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Kunming 650500, P.R. China.
authors.forename: Junmei
authors.initials: J
authors.lastname: Ding
authors.affiliation: School of Life Science, Yunnan Normal University, Kunming 650500, P.R. China.|Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650500, P.R. China.|Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Kunming 650500, P.R. China.
authors.forename: Nanyu
authors.initials: N
authors.lastname: Han
authors.affiliation: School of Life Science, Yunnan Normal University, Kunming 650500, P.R. China.|Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650500, P.R. China.|Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Kunming 650500, P.R. China.
authors.forename: Zunxi
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D016673:Colobinae / Q000382:microbiology*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004795:Enzyme Stability; D005243:Feces / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D042802:Oxalobacteraceae / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012965:Sodium Chloride; D043364:Endo-1,4-beta Xylanases
keywords: Gastrointestinal tract; Massilia; Rhinopithecus bieti; Xylanase; salt tolerant