title: A two-stage fermentation process of erythritol production by yeast Y. lipolytica from molasses and glycerol.
abstract: In this study, a two-stage fermentation process of erythritol production based on molasses and glycerol was investigated. During the first stage, the biomass of Yarrowia lipolytica was grown on medium containing sucrose as the sole carbon source. In the second stage, production of erythritol was initiated by glycerol addition. To use molasses as a substrate for erythritol synthesis, sucrose utilization was established by expressing the Saccharomyces cerevisiae SUC2 gene. In this study, cultivation of yeast Y. lipolytica could produce 52-114 g/L of erythritol. The productivity was 0.58-1.04 g/L/h, and yield was 0.26-0.57 g/g; the final biomasses yield ranged 17-41 g/L. This is the first report describing erythritol production via industrial raw molasses and glycerol by Y. lipolytica. This work uses genetically modified strains of Y. lipolytica as tool for the direct conversion of affordable raw industrial molasses and glycerol into the value-added erythritol product.
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, Wrocław 51-630, Poland.
authors.forename: Aleksandra M
authors.initials: AM
authors.lastname: Mirończuk
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, Wrocław 51-630, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Rakicka
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, Wrocław 51-630, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Biegalska
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, Wrocław 51-630, Poland.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Rymowicz
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, Wrocław 51-630, Poland. Electronic address: adam.dobrowolski@up.wroc.pl.
authors.forename: Adam
authors.initials: A
authors.lastname: Dobrowolski
mesh_terms: D018533:Biomass; D004896:Erythritol / Q000096:biosynthesis*; D005285:Fermentation / Q000502:physiology*; D005990:Glycerol; D008965:Molasses; D012441:Saccharomyces cerevisiae; D025062:Yarrowia / Q000378:metabolism*
chemical_list: D005990:Glycerol; D004896:Erythritol
keywords: Erythritol; Glycerol; Molasses; Yarrowia lipolytica