title: A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease.
abstract: We present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select for target proteins of interest and analyze them using molecular microscopy. Implementing our new streamlined microchip approach, we could directly visualize BRCA1 gene regulatory complexes from patient-derived cancer cells for the first time.
authors.affiliation: Virginia Tech Carilion Research Institute, 2 Riverside Circle, Roanoke, Virginia 24016, United States.
authors.forename: Brian L
authors.initials: BL
authors.lastname: Gilmore
authors.affiliation: Virginia Tech Carilion Research Institute, 2 Riverside Circle, Roanoke, Virginia 24016, United States.|School of Biomedical Engineering and Science, Virginia Tech, Blacksburg, VA 24061, United States.
authors.forename: Carly E
authors.initials: CE
authors.lastname: Winton
authors.affiliation: Virginia Tech Carilion Research Institute, 2 Riverside Circle, Roanoke, Virginia 24016, United States.|Virginia Tech Carilion School of Medicine, 2 Riverside Circle, Roanoke, Virginia 24016, United States.
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Demmert
authors.affiliation: Virginia Tech Carilion Research Institute, 2 Riverside Circle, Roanoke, Virginia 24016, United States.
authors.forename: Justin R
authors.initials: JR
authors.lastname: Tanner
authors.affiliation: Virginia Tech Carilion Research Institute, 2 Riverside Circle, Roanoke, Virginia 24016, United States.
authors.forename: Sam
authors.initials: S
authors.lastname: Bowman
authors.affiliation: Virginia Tech Carilion Research Institute, 2 Riverside Circle, Roanoke, Virginia 24016, United States.
authors.forename: Vasilea
authors.initials: V
authors.lastname: Karageorge
authors.affiliation: Virginia Tech Carilion Research Institute, 2 Riverside Circle, Roanoke, Virginia 24016, United States.
authors.forename: Kaya
authors.initials: K
authors.lastname: Patel
authors.affiliation: Virginia Tech Carilion Research Institute, 2 Riverside Circle, Roanoke, Virginia 24016, United States.|School of Biomedical Engineering and Science, Virginia Tech, Blacksburg, VA 24061, United States.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Sheng
authors.affiliation: Virginia Tech Carilion Research Institute, 2 Riverside Circle, Roanoke, Virginia 24016, United States.|School of Biomedical Engineering and Science, Virginia Tech, Blacksburg, VA 24061, United States.|Virginia Tech Carilion School of Medicine, 2 Riverside Circle, Roanoke, Virginia 24016, United States.|Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061, United States.
authors.forename: Deborah F
authors.initials: DF
authors.lastname: Kelly
mesh_terms: D019313:BRCA1 Protein / Q000235:genetics / Q000378:metabolism*; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D011487:Protein Conformation; D012319:RNA Polymerase II / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D019313:BRCA1 Protein; C492913:BRCA1 protein, human; D025521:Tumor Suppressor Proteins; D025801:Ubiquitin; C102449:BARD1 protein, human; D044767:Ubiquitin-Protein Ligases; D012319:RNA Polymerase II