title: Proposal to optimize ecotoxicological evaluation of wastewater treated by conventional biological and ozonation processes.
abstract: A mixture of urban and hospital effluents (50% v/v) was evaluated for ecotoxicity with an advanced bioassay battery. Mixed effluents were tested before any treatment, after biological treatment alone, and after biological treatment followed by a tertiary ozonation (15 mg O3/L). Laying a high value on the continuance of organisms' fitness, essential to preserve a healthy receiving ecosystem, the main objective of this study was to combine normalized bioassays with newly developed in vivo and in vitro tests in order to assess alteration of embryo development, growth and reproduction, as well as genotoxic effects in aquatic organisms exposed to complex wastewater effluents. Comparison of the bioassays sensitivity was considered. Contrary to the lack of toxicity observed with normalized ecotoxicity tests, endpoints measured on zebrafish embryos such as developmental abnormalities and genotoxicity demonstrated a residual toxicity in wastewater both after a biological treatment followed or not by a tertiary O3 treatment. However, the ozonation step allowed to alleviate the residual endocrine disrupting potential measure in the biologically treated effluent. This study shows that normalized bioassays are not sensitive enough for the ecotoxicological evaluation of wastewaters and that there is a great need for the development of suitable sensitive bioassays in order to characterize properly the possible residual toxicity of treated effluents.
authors.affiliation: UMR 5023 LEHNA, INRA, ENTPE, rue Maurice Audin, 69518, Vaulx-en-Velin Cedex, France.
authors.forename: Adriana
authors.initials: A
authors.lastname: Wigh
authors.affiliation: UMR 5023 LEHNA, INRA, ENTPE, rue Maurice Audin, 69518, Vaulx-en-Velin Cedex, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Devaux
authors.affiliation: UMR 5023 LEHNA, INRA, ENTPE, rue Maurice Audin, 69518, Vaulx-en-Velin Cedex, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Brosselin
authors.affiliation: SUEZ Traitement de l'eau, Direction Technique Innovation, Degrémont SAS 183, avenue du 18 juin 1940, 92508, Rueil-Malmaison, France.
authors.forename: Adriana
authors.initials: A
authors.lastname: Gonzalez-Ospina
authors.affiliation: SUEZ Traitement de l'eau, Direction Technique Innovation, Degrémont SAS 183, avenue du 18 juin 1940, 92508, Rueil-Malmaison, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Domenjoud
authors.affiliation: INERIS, Unité d'Ecotoxicologie in vitro et in vivo (ECOT), BP2, 60550, Verneuil-en-Halatte, France.
authors.forename: Selim
authors.initials: S
authors.lastname: Aït-Aïssa
authors.affiliation: INERIS, Unité d'Ecotoxicologie in vitro et in vivo (ECOT), BP2, 60550, Verneuil-en-Halatte, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Creusot
authors.affiliation: UMR 5023 LEHNA, INRA, ENTPE, rue Maurice Audin, 69518, Vaulx-en-Velin Cedex, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Gosset
authors.affiliation: PROVADEMSE, Boulevard Niels Bohr, CS 52132, 69603, Villeurbanne Cedex, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Bazin
authors.affiliation: UMR 5023 LEHNA, INRA, ENTPE, rue Maurice Audin, 69518, Vaulx-en-Velin Cedex, France. bony@entpe.fr.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bony
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D001681:Biological Assay / Q000379:methods; D002460:Cell Line; D003445:Crustacea / Q000187:drug effects / Q000254:growth & development; D054750:Ecotoxicology; D004625:Embryo, Nonmammalian / Q000187:drug effects; D052244:Endocrine Disruptors / Q000032:analysis* / Q000633:toxicity; D006801:Humans; D010126:Ozone / Q000737:chemistry*; D010865:Pilot Projects; D011960:Receptors, Estrogen / Q000378:metabolism; D012403:Rotifera / Q000187:drug effects / Q000254:growth & development; D018675:Toxicity Tests / Q000379:methods; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity; D018508:Water Purification / Q000379:methods*; D015027:Zebrafish / Q000254:growth & development
chemical_list: D052244:Endocrine Disruptors; D010126:Ozone; D011960:Receptors, Estrogen; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Bioassay; Ecotoxicity; Fish embryo test; Genotoxicity; Micropollutant; Ozonation; Wastewater