title: Gene expression profile of necrotizing enterocolitis model in neonatal mice.
abstract: BACKGROUND
  Necrotizing enterocolitis (NEC) characterized by intestinal necrosis is one of the most common gastrointestinal emergencies in newborns. The main purpose of this study was to evaluate the whole genome expression levels in a NEC mouse model controlled with breast milk.
  
  
  METHODS
  This study induced a NEC model in mice of gestational ages of 18-21 days by intensive hypoxic insult and permitted breast-feeding instead of formula feeding. After evaluating the NEC status in the small intestines of neonatal mice by histological examination, a genome-wide gene expression profile study was completed using microarray analysis.
  
  
  RESULTS
  A total of 72 genes (38 down-regulated and 34 up-regulated) were observed to have significantly different expression profiles in the NEC mouse model compared with the normal control animals, based on a significance at fold change ≥ 2 and P < 0.05. In particular, down-regulated Hist1h2aa and up-regulated Ube2i showed the most significant signals (P = 0.0008 for both genes). In an additional gene ontology analysis, the endopeptidase related categories (specifically, serine-type endopeptidase inhibitor activity, P = 8.95 × 10(-5); Pcorr = 0.008) appeared to affect NEC development in the mouse model.
  
  
  CONCLUSIONS
  Although replications and functional evaluations are needed, our results suggest that several genes may have different expression profiles in the NEC mouse model. In particular, endopeptidase related genes (which are also known to be relevant to NEC), as identified through gene ontology analysis, may represent attractive targets for future research.
authors.affiliation: Department of Surgery, Seoul National University Bundang Hospital, Seongnam, Gyeonggi, 463-707, Republic of Korea.
authors.forename: Kyuwhan
authors.initials: K
authors.lastname: Jung
authors.affiliation: Research Institute for Basic Science, Sogang University, Seoul, 121-742, Republic of Korea.
authors.forename: Jeong-Hyun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Genetic Epidemiology, SNP Genetics, Inc., Seoul 153-803, Republic of Korea.
authors.forename: Hyun Sub
authors.initials: HS
authors.lastname: Cheong
authors.affiliation: Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi, 463-707, Republic of Korea.
authors.forename: Eun
authors.initials: E
authors.lastname: Shin
authors.affiliation: Department of Surgery, Seoul National University Bundang Hospital, Seongnam, Gyeonggi, 463-707, Republic of Korea.
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Preclinical Experimental Center, Seoul National University Bundang Hospital, Seongnam, Gyeonggi, 463-707, Republic of Korea.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Hwang
authors.affiliation: Department of Surgery, Seoul National University Bundang Hospital, Seongnam, Gyeonggi, 463-707, Republic of Korea.
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Lee
authors.affiliation: Department of Surgery, Seoul National University Bundang Hospital, Seongnam, Gyeonggi, 463-707, Republic of Korea.
authors.forename: Mi-Ok
authors.initials: MO
authors.lastname: Yoon
authors.affiliation: Department of Surgery, Seoul National University Bundang Hospital, Seongnam, Gyeonggi, 463-707, Republic of Korea.
authors.forename: Seong-Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Surgery, Seoul National University Bundang Hospital, Seongnam, Gyeonggi, 463-707, Republic of Korea.
authors.forename: Cherry Ann
authors.initials: CA
authors.lastname: Sio
authors.affiliation: Research Institute for Basic Science, Sogang University, Seoul, 121-742, Republic of Korea; Department of Life Science, Sogang University, Seoul 121-742, Republic of Korea. Electronic address: hdshin@sogang.ac.kr.
authors.forename: Hyoung Doo
authors.initials: HD
authors.lastname: Shin
authors.affiliation: Department of Surgery, Seoul National University Children's Hospital, Seoul 110-773, Republic of Korea. Electronic address: sejung@snu.ac.kr.
authors.forename: Sung-Eun
authors.initials: SE
authors.lastname: Jung
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D010450:Endopeptidases / Q000235:genetics; D020345:Enterocolitis, Necrotizing / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D000860:Hypoxia; D051379:Mice; D059467:Transcriptome
chemical_list: D010450:Endopeptidases
keywords: Gene expression; Mouse model; Necrotizing enterocolitis (NEC)