title: A novel electrochemical immunosensor using β-cyclodextrins functionalized silver supported adamantine-modified glucose oxidase as labels for ultrasensitive detection of alpha-fetoprotein.
abstract: In this work, a novel sandwich-type electrochemical immunosensor based on host-guest interaction was fabricated for the detection of alpha-fetoprotein (AFP). Due to the large specific surface area of multiwalled carbon nanotubes and the unique supramolecular recognition ability of β-cyclodextrins, ferrocenecarboxylic acid (Fc) was incorporated into this sensor platform by host-guest interaction to generate an electrochemical signal. And β-cyclodextrins functionalized silver supported adamantine-modified glucose oxidase (GOD-CD-Ag), was used as a label to improve the analytical performance of the immunosensor by the dual amplification strategy. The obtained GOD-CD-Ag conjugates could convert glucose into gluconic acid with the formation of hydrogen peroxide (H2O2). And then silver nanoparticles could in situ catalyze the reduction of the generated H2O2, dramatically improving the oxidation reaction of Fc. The developed immunosensor shows a wide linear calibration range from 0.001 to 5.0 ng/mL with a low detection limit (0.2 pg/mL) for the detection of AFP. The method, with ideal reproducibility and selectivity, has a wide application prospect in clinical research.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Lv
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yan
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address: sdjndxwq@163.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005296:Ferrous Compounds / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay; D053768:Metal Nanoparticles / Q000737:chemistry; D000075163:Metallocenes; D037742:Nanotubes, Carbon / Q000737:chemistry; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000906:Antibodies; D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D000075163:Metallocenes; D037742:Nanotubes, Carbon; D000509:alpha-Fetoproteins; D047392:beta-Cyclodextrins; C032949:ferrocenecarboxylic acid; D012834:Silver; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase
keywords: Alpha-fetoprotein; Dual amplification; Functionalized multiwalled carbon nanotubes; Host-guest interaction; β-cyclodextrins