title: Downregulation of Plasma MiR-142-3p and MiR-26a-5p in Patients With Colorectal Carcinoma.
abstract: BACKGROUND
  Colorectal cancer is one of the most commonly diagnosed cancers and cancer- related death worldwide. Identification of new specific biomarkers could be helpful to detection of this malignancy. Altered plasma microRNA expression has been identified in many cancers, including colorectal cancer.
  
  
  OBJECTIVE
  The main objective of this study was to identify the circulating microRNAs with the most expression changes in colorectal cancer patients compared with neoplasm free healthy individuals.
  
  
  METHODS
  MicroRNA expression profiling was performed on plasma samples of 37 colorectal cancer patients and 8 normal subjects using microRNA microarray. Quantitative real-time reverse transcription polymerase chain reaction was used to validate the two selected altered microR NAs. Plasma samples from 61 colorectal cancer patients and 24 normal subjects were used in our validation study.
  
  
  RESULTS
  In profiling study we found a panel of six plasma microRNAs with significant downregulation. MicroRNA-142-3p and microRNA-26a-5p were selected and validated by polymerase chain reaction. Our results demonstrated that expression levels of plasma microRNA-142-3p and microRNA-26a-5p were significantly downregulated in patients with colorectal cancer when compared to control group.
  
  
  CONCLUSIONS
  Our findings suggest that downregulation of plasma microRNA-142-3p and microRNA-26a-5p might serve as novel noninvasive biomarkers in the diagnosis of colorectal cancer, although more studies are needed to highlight the theoretical strengths.
authors.affiliation: Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, IR Iran ; Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ghanbari
authors.affiliation: Department of Pathology, Haartman Institute, Helsinki University, Helsinki, Finland.
authors.forename: Neda
authors.initials: N
authors.lastname: Mosakhani
authors.affiliation: Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran.
authors.forename: Jahanbakhsh
authors.initials: J
authors.lastname: Asadi
authors.affiliation: Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Moddares University, Tehran, IR Iran.
authors.forename: Nazila
authors.initials: N
authors.lastname: Nouraee
authors.affiliation: Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Moddares University, Tehran, IR Iran.
authors.forename: Seyed Javad
authors.initials: SJ
authors.lastname: Mowla
authors.affiliation: Infectious Disease Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran.
authors.forename: Yaghoub
authors.initials: Y
authors.lastname: Yazdani
authors.affiliation: Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Ashraf
authors.initials: A
authors.lastname: Mohamadkhani
authors.affiliation: Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Poustchi
authors.affiliation: Department of Pathology, Haartman Institute, Helsinki University, Helsinki, Finland.
authors.forename: Sakari
authors.initials: S
authors.lastname: Knuutila
authors.affiliation: Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Malekzadeh
keywords: Biomarkers; Circulating microRNA; Early Detection; Microarray