title: Effectiveness of non-biodegradable poly(2-hydroxyethyl methacrylate)-based hydrogel particles as a fibroblast growth factor-2 releasing carrier.
abstract: OBJECTIVE
  Dental resin-based restorative materials are used in a variety of dental treatment modalities such as root-end filling, perforation sealing, and adhesion of fractured roots. However, the prognosis after such treatments is not necessarily favorable because they fail to promote healing of the surrounding alveolar tissue. In the present study, non-biodegradable poly-2-hydroxyethyl methacrylate (polyHEMA)-based hydrogel particles were fabricated as a carrier vehicle for drug delivery that is applied to dental resins.
  
  
  METHODS
  The loading and release characteristics of bovine serum albumin (BSA) and fibroblast growth factor-2 (FGF-2) from the polyHEMA-based hydrogel particles were evaluated over time in culture. The hydrogel particles were immersed into an aqueous FITC-labeled BSA solution and were observed using confocal laser scanning microscopy (CLSM). To determine the activity of the FGF-2 released from the particles, the proliferation of osteoblast-like cells cultured with eluates collected from the particles for up to 14 days was determined.
  
  
  RESULTS
  CLSM revealed that BSA was adsorbed to the surface of the hydrogel particles. A sustained release of BSA and FGF-2 from the particles was detected for up to 14 days. The eluates from the FGF-2-loaded particles increased the proliferation of the osteoblast-like cells, suggesting that the activity of FGF-2 was maintained for at least 2 weeks within the particles.
  
  
  CONCLUSIONS
  These polyHEMA-based non-degradable hydrogel particles may be useful tools that can be applied to dental restorative materials to achieve sustained delivery of drugs that promote tissue regeneration.
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Kahoru
authors.initials: K
authors.lastname: Takeda
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Haruaki
authors.initials: H
authors.lastname: Kitagawa
authors.affiliation: Department of Biomaterials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Ririko
authors.initials: R
authors.lastname: Tsuboi
authors.affiliation: Department of Biomaterials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Wakako
authors.initials: W
authors.lastname: Kiba
authors.affiliation: Department of Biomaterials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Sasaki
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Mikako
authors.initials: M
authors.lastname: Hayashi
authors.affiliation: Department of Biomaterials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: imazato@dent.osaka-u.ac.jp.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Imazato
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D004337:Drug Carriers; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D020100:Hydrogels / Q000494:pharmacology*; D008689:Methacrylates; D051379:Mice; D053761:Nanocomposites; D010006:Osteoblasts / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D020100:Hydrogels; D008689:Methacrylates; D016222:Fibroblast Growth Factor 2; C005044:hydroxyethyl methacrylate
keywords: FGF-2; Hydrogel; Osteoblast-like cell; Resin-based material; Tissue regeneration