title: Physapubescin B Exhibits Potent Activity against Human Prostate Cancer In Vitro and In Vivo.
abstract: The present data showed that a natural compound isolated from the plant Physalis pubescens L. (Solanaceae), physapubescin B, exhibited antitumor activity against prostate cancer in vitro and in vivo. Treating prostate cancer cells with physapubescin B resulted in the accumulation of cells in the G2/M phase, which was associated with reduced Cdc25C levels and increased levels of CyclinB1, P21 as well as p-Cdk1 (Tyr15). Additionally, reactive oxygen species (ROS) generation was increased in physapubescin B-treated PC-3 cells. Furthermore, the physapubescin B-induced decrease of Cdc25C protein expression together with the G2/M phase cell cycle arrest were significantly abrogated by antioxidant NAC and GSH. Our data also demonstrated that physapubescin B exhibited strong in vivo antitumor efficacy in human prostate cancer PC3 xenograft. In conclusion, our results provide clear evidence that physapubescin B exhibits antitumor activity both in vitro and in vivo and deserves further development as an anticancer agent.
authors.affiliation: Institute of Marine Biology, Ocean College of Zhejiang University , Hangzhou, Zhejiang 310058, China.
authors.forename: Wanjing
authors.initials: W
authors.lastname: Ding
authors.affiliation: Institute of Marine Biology, Ocean College of Zhejiang University , Hangzhou, Zhejiang 310058, China.
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Pharmacology, School of Basic Medical Sciences, Zhejiang University , Hangzhou, Zhejiang 310058, China.
authors.forename: Zhewen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Marine Biology, Ocean College of Zhejiang University , Hangzhou, Zhejiang 310058, China.
authors.forename: Qiaoqiao
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Department of Pharmacology, School of Basic Medical Sciences, Zhejiang University , Hangzhou, Zhejiang 310058, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Tang
authors.affiliation: Institute of Marine Biology, Ocean College of Zhejiang University , Hangzhou, Zhejiang 310058, China.
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Ma
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008297:Male; D008819:Mice, Nude; D032321:Physalis / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D054358:Withanolides / Q000494:pharmacology*; D020687:cdc25 Phosphatases / Q000235:genetics / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D017382:Reactive Oxygen Species; D054358:Withanolides; C586240:physapubescin B; D020687:cdc25 Phosphatases
keywords: Cdc25C; G2/M phase arrest; ROS; antitumor activity; physapubescin B; prostate cancer