title: SCARECROW-LIKE23 and SCARECROW jointly specify endodermal cell fate but distinctly control SHORT-ROOT movement.
abstract: Intercellular signaling through trafficking of regulatory proteins is a widespread phenomenon in plants and can deliver positional information for the determination of cell fate. In the Arabidopsis root meristem, the cell fate determinant SHORT-ROOT (SHR), a GRAS domain transcription factor, acts as a signaling molecule from the stele to the adjacent layer to specify endodermal cell fate. Upon exiting the stele, SHR activates another GRAS domain transcription factor, SCARCROW (SCR), which, together with several BIRD/INDETERMINATE DOMAIN proteins, restricts movement of SHR to define a single cell layer of endodermis. Here we report that endodermal cell fate also requires the joint activity of both SCR and its closest homologue SCARECROW-LIKE23 (SCL23). We show that SCL23 protein moves with zonation-dependent directionality. Within the meristem, SCL23 exhibits short-ranged movement from ground tissue to vasculature. Away from the meristem, SCL23 displays long-range rootward movement into meristematic vasculature and a bidirectional radial spread, respectively. As a known target of SHR and SCR, SCL23 also interacts with SCR and SHR and can restrict intercellular outspread of SHR without relying on nuclear retention as SCR does. Collectively, our data show that SCL23 is a mobile protein that controls movement of SHR and acts redundantly with SCR to specify endodermal fate in the root meristem.
authors.affiliation: Plant Developmental Biology, Plant Sciences, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen, 6708PB, the Netherlands.|Molecular Genetics, Department Biology, Padualaan 8, Utrecht, 3581CH, the Netherlands.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Long
authors.affiliation: Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, the Netherlands.
authors.forename: Joachim
authors.initials: J
authors.lastname: Goedhart
authors.affiliation: Molecular Genetics, Department Biology, Padualaan 8, Utrecht, 3581CH, the Netherlands.
authors.forename: Martinus
authors.initials: M
authors.lastname: Schneijderberg
authors.affiliation: Molecular Genetics, Department Biology, Padualaan 8, Utrecht, 3581CH, the Netherlands.
authors.forename: Inez
authors.initials: I
authors.lastname: Terpstra
authors.affiliation: Molecular Genetics, Department Biology, Padualaan 8, Utrecht, 3581CH, the Netherlands.
authors.forename: Akie
authors.initials: A
authors.lastname: Shimotohno
authors.affiliation: Cell Biology, Department Biology, Utrecht University, Padualaan 8, Utrecht, 3581CH, the Netherlands.
authors.forename: Benjamin P
authors.initials: BP
authors.lastname: Bouchet
authors.affiliation: Cell Biology, Department Biology, Utrecht University, Padualaan 8, Utrecht, 3581CH, the Netherlands.
authors.forename: Anna
authors.initials: A
authors.lastname: Akhmanova
authors.affiliation: Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, the Netherlands.
authors.forename: Theodorus W J
authors.initials: TW
authors.lastname: Gadella
authors.affiliation: Plant Developmental Biology, Plant Sciences, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen, 6708PB, the Netherlands.|Molecular Genetics, Department Biology, Padualaan 8, Utrecht, 3581CH, the Netherlands.
authors.forename: Renze
authors.initials: R
authors.lastname: Heidstra
authors.affiliation: Plant Developmental Biology, Plant Sciences, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen, 6708PB, the Netherlands.|Molecular Genetics, Department Biology, Padualaan 8, Utrecht, 3581CH, the Netherlands.
authors.forename: Ben
authors.initials: B
authors.lastname: Scheres
authors.affiliation: Plant Developmental Biology, Plant Sciences, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen, 6708PB, the Netherlands.|Molecular Genetics, Department Biology, Padualaan 8, Utrecht, 3581CH, the Netherlands.
authors.forename: Ikram
authors.initials: I
authors.lastname: Blilou
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement / Q000235:genetics; D031541:Fluorescence Resonance Energy Transfer; D018506:Gene Expression Regulation, Plant; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D018519:Meristem / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D018520:Plant Shoots / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D058526:Plant Vascular Bundle / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011485:Protein Binding; D021381:Protein Transport; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D008164:Luminescent Proteins; C000594055:SCL23 protein, Arabidopsis; C100978:SCR protein, Arabidopsis; C410510:SHORT ROOT protein, Arabidopsis; D014157:Transcription Factors
keywords: Arabidopsis thaliana; SCL23; SCR-SHR complex; endodermal fate; intercellular movement; mobile protein; protein interaction