title: Reusable and dual-potential responses electrogenerated chemiluminescence biosensor for synchronously cytosensing and dynamic cell surface N-glycan evaluation.
abstract: A novel reusable and dual-potential responsive electrogenerated chemiluminescence (ECL) biosensor was fabricated for synchronous detection of cancer cells and their surface N-glycan. In this strategy, a cancer cell recognized aptamer hybridized with a capture DNA was immobilized on electrochemically reduced MoS2 nanosheets, and Ru(phen)3(2+) as ECL probes was intercalated into the grooves of the double-strand DNA. In the presence of target cells, the capture DNA and Ru(phen)3(2+) were released from the electrode interface owing to the specific interaction between cancer cells and the aptamer. Meanwhile, concanavalin A (Con A), a mannose binding protein, and a conjugated gold nanoparticle modified graphite-C3N4 (Con A@Au-C3N4) was used as a negative ECL nanoprobe and applied for the cell surface N-glycan evaluation owing to the excellent ECL properties of g-C3N4 at negative potential. The cytosensing and cell surface N-glycan evaluation could be simultaneously realized with high sensitivity and excellent selectivity based on the ratio of ECL intensity between the negative potential and positive potential (ΔECLn/ΔECLp), avoiding the traditional routing cell counting procedures. Moreover, the aptamer modified electrode can be regenerated in the presence of capture DNA solutions for cyclic utilization. As a proof-of-concept, the ECL cytosensor showed excellent performances for the analysis of the MCF-7 cancer cell and its surface N-glycan evaluation in human serum samples. The reusable and dual potential response ECL biosensor endows a feasibility tool for clinical diagnosis and drug screening especially in complex biological systems.
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Analytical Methods and Instrumentation, Tsinghua University , Beijing 100084, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Analytical Methods and Instrumentation, Tsinghua University , Beijing 100084, China.
authors.forename: Jinghong
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Analytical Methods and Instrumentation, Tsinghua University , Beijing 100084, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry* / Q000473:pathology; D004220:Disulfides / Q000737:chemistry; D004867:Equipment Design; D006801:Humans; D049449:Luminescence; D049408:Luminescent Agents / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation*; D008982:Molybdenum / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D009369:Neoplasms / Q000737:chemistry* / Q000473:pathology; D009942:Organometallic Compounds / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D004220:Disulfides; D049408:Luminescent Agents; D009942:Organometallic Compounds; D010618:Phenanthrolines; D011134:Polysaccharides; C034282:tris-(1,10-phenanthroline)ruthenium; D008982:Molybdenum; C082964:molybdenum disulfide