title: The development of a cholesterol biosensor using a liquid crystal/aqueous interface in a SDS-included β-cyclodextrin aqueous solution.
abstract: Sodium dodecyl sulphate (SDS) including β-cyclodextrin (β-CD) (β-CDSDS) was used to detect cholesterol at the 4-cyano-4'-pentylbiphenyl (5CB)/aqueous interface in transmission electron microscopy (TEM) grid cells. The β-CD acts as a host for SDS (guest). The guest SDS enclosed within the β-CD cavity was replaced with cholesterol by injecting cholesterol solution into the TEM cell at concentrations greater than 3 μM. The replacement of SDS with cholesterol was confirmed by pH measurement and high performance liquid chromatography (HPLC). The SDS excluded from the β-CD altered the planar orientation of the 5CB confined within the TEM grid cell to a homeotropic orientation. This planar-to-homeotropic transition was observed using a polarized optical microscope under crossed polarizers. This convenient TEM grid cell provides a new method for the selective detection of cholesterol without immobilization of the detecting receptors (enzyme, antibody, or aptamer) or the use of sophisticated instruments.
authors.affiliation: School of Applied Chemical Engineering, Department of Polymer Science & Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Sundas
authors.initials: S
authors.lastname: Munir
authors.affiliation: School of Applied Chemical Engineering, Department of Polymer Science & Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic address: psy@knu.ac.kr.
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Park
mesh_terms: D015374:Biosensing Techniques; D002784:Cholesterol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D050866:Liquid Crystals / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D047392:beta-Cyclodextrins; D014867:Water; D012967:Sodium Dodecyl Sulfate; D002784:Cholesterol; C031215:betadex
keywords: Biosensor; Cholesterol; Liquid crystal; Sodium dodecyl sulphate; β-cyclodextrin