title: Statistical modeling of pullulan production and its application in pullulan acetate nanoparticles synthesis.
abstract: This work focused on the optimization of exo-polysaccharide, pullulan production by exploiting cassava bagasse, an agricultural solid waste residue by solid state fermentation and its application in the preparation of pullulan acetate nanoparticles. Statistical approach was investigated to maximize the pullulan production using C/N ratio, initial pH, NaCl and ZnSO4·5H2O. The optimum conditions for maximum yield of pullulan (39.42±0.62mg/gds) were found to be: C/N=25.94, initial pH=5.5 and NaCl=0.55g/L. Using the optimized medium variables, the production of pullulan was investigated in lab scale solid state fermentation. The pullulan produced was characterized by thermo gravimetric and XRD analysis. Also pullulan acetate nanoparticles were synthesized from chemical modification of pullulan and the average particle size of nanoparticles was examined by zeta particle sizer.
authors.affiliation: Bioprocess Engineering Laboratory, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamilnadu, India. Electronic address: sugumaransastra@outlook.com.
authors.forename: K R
authors.initials: KR
authors.lastname: Sugumaran
authors.affiliation: Bioprocess Engineering Laboratory, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamilnadu, India.
authors.forename: V
authors.initials: V
authors.lastname: Ponnusami
mesh_terms: D000085:Acetates / Q000138:chemical synthesis*; D000704:Analysis of Variance; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D005936:Glucans / Q000096:biosynthesis / Q000138:chemical synthesis*; D015233:Models, Statistical; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D066244:Proton Magnetic Resonance Spectroscopy; D013818:Thermogravimetry; D013997:Time Factors; D014961:X-Ray Diffraction
chemical_list: D000085:Acetates; D005936:Glucans; D002244:Carbon; C009109:pullulan
keywords: Cassava bagasse; Pullulan; Pullulan acetate nanoparticles