title: Biodegradation of Decabromodiphenyl Ether (BDE-209) by Crude Enzyme Extract from Pseudomonas aeruginosa.
abstract: The biodegradation effect and mechanism of decabromodiphenyl ether (BDE-209) by crude enzyme extract from Pseudomonas aeruginosa were investigated. The results demonstrated that crude enzyme extract exhibited obviously higher degradation efficiency and shorter biodegradation time than Pseudomonas aeruginosa itself. Under the optimum conditions of pH 9.0, 35 °C and protein content of 2000 mg/L, 92.77% of the initial BDE-209 (20 mg/L) was degraded after 5 h. A BDE-209 biodegradation pathway was proposed on the basis of the biodegradation products identified by GC-MS analysis. The biodegradation mechanism showed that crude enzyme extract degraded BDE-209 into lower brominated PBDEs and OH-PBDEs through debromination and hydroxylation of the aromatic rings.
authors.affiliation: School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China. liuyu2007@yahoo.com.|Dapartment of Biotechnology, Daqing Branch of Heilongjiang Academy of Science, Daqing 163319, China. liuyu2007@yahoo.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China. gongaijun5661@ustb.edu.cn.
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Gong
authors.affiliation: School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China. qiulina@ustb.edu.cn.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Qiu
authors.affiliation: School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China. l460919452@163.com.
authors.forename: Jing-Rui
authors.initials: JR
authors.lastname: Li
authors.affiliation: School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China. FK_liHarrison@163.com.
authors.forename: Fu-Kai
authors.initials: FK
authors.lastname: Li
mesh_terms: D001673:Biodegradation, Environmental; D004785:Environmental Pollutants / Q000378:metabolism*; D052918:Environmental Restoration and Remediation / Q000379:methods*; D005411:Flame Retardants / Q000378:metabolism; D055768:Halogenated Diphenyl Ethers / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
chemical_list: D004785:Environmental Pollutants; D005411:Flame Retardants; D055768:Halogenated Diphenyl Ethers; C010902:decabromobiphenyl ether
keywords: BDE-209; Pseudomonas aeruginosa; biodegradation; biodegradation mechanism; crude enzyme extract