title: Comparison among different cryoprotectants for cryopreservation of epididymal sperm from agouti (Dasyprocta leporina).
abstract: We verify the effects of different cryoprotectants on the cryopreservation of agouti (Dasyprocta leporina) epididymal sperm. We used 16 pairs of testes-epididymis complexes of sexually mature animals. We immediately evaluated epididymal sperm obtained by retrograde flushing for concentration, motility, vigor, viability, osmotic response, and morphology. Samples were extended in a coconut water extender plus 20% egg yolk, containing glycerol, ethylene glycol, dimethylsulfoxide - DMSO, or dimethylformamide. Finally, samples were stored in 0.25 mL straws, frozen in liquid nitrogen, and thawed after one week, being reevaluated and assessed for membrane integrity using fluorescent probes. The higher values for postthawing sperm motility, vigor, and membrane integrity were achieved by the usage of glycerol, when compared to ethylene glycol and dimethylformamide (P < 0.05); however, no differences were found between glycerol and DMSO (P > 0.05). All cryoprotectants provided a similar effect on the preservation of sperm morphology, osmotic response, and viability (P > 0.05). Therefore, here onwards, there was testing of glycerol and DMSO at 3 and 6% concentrations using the same freezing-thawing protocol reported previously. As the main result, DMSO at 6% concentration provided a decrease in sperm parameters, as well as in the chromatin integrity and in the binding capability of sperm. In conclusion, glycerol 3 or 6% and DMSO 3% can be used as alternative cryoprotectants for agouti epididymal sperm cryopreservation.
authors.affiliation: Laboratório de Conservação de Germoplasma Animal, Universidade Federal Rural do Semi-Árido, BR 110, Km 47, Costa e Silva, 59625-900 Mossoró, RN, Brazil.
authors.forename: T S
authors.initials: TS
authors.lastname: Castelo
authors.affiliation: Laboratório de Conservação de Germoplasma Animal, Universidade Federal Rural do Semi-Árido, BR 110, Km 47, Costa e Silva, 59625-900 Mossoró, RN, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: Silva
authors.affiliation: Laboratório de Conservação de Germoplasma Animal, Universidade Federal Rural do Semi-Árido, BR 110, Km 47, Costa e Silva, 59625-900 Mossoró, RN, Brazil.
authors.forename: L G P
authors.initials: LG
authors.lastname: Bezerra
authors.affiliation: Laboratório de Conservação de Germoplasma Animal, Universidade Federal Rural do Semi-Árido, BR 110, Km 47, Costa e Silva, 59625-900 Mossoró, RN, Brazil.
authors.forename: C Y M
authors.initials: CY
authors.lastname: Costa
authors.affiliation: Laboratório de Conservação de Germoplasma Animal, Universidade Federal Rural do Semi-Árido, BR 110, Km 47, Costa e Silva, 59625-900 Mossoró, RN, Brazil.
authors.forename: A E A
authors.initials: AE
authors.lastname: Lago
authors.affiliation: Laboratório de Conservação de Germoplasma Animal, Universidade Federal Rural do Semi-Árido, BR 110, Km 47, Costa e Silva, 59625-900 Mossoró, RN, Brazil.
authors.forename: J A B
authors.initials: JA
authors.lastname: Bezerra
authors.affiliation: Laboratório de Conservação de Germoplasma Animal, Universidade Federal Rural do Semi-Árido, BR 110, Km 47, Costa e Silva, 59625-900 Mossoró, RN, Brazil.
authors.forename: L B
authors.initials: LB
authors.lastname: Campos
authors.affiliation: Laboratório de Conservação de Germoplasma Animal, Universidade Federal Rural do Semi-Árido, BR 110, Km 47, Costa e Silva, 59625-900 Mossoró, RN, Brazil.
authors.forename: E C G
authors.initials: EC
authors.lastname: Praxedes
authors.affiliation: Laboratório de Conservação de Germoplasma Animal, Universidade Federal Rural do Semi-Árido, BR 110, Km 47, Costa e Silva, 59625-900 Mossoró, RN, Brazil. Electronic address: legio2000@yahoo.com.
authors.forename: A R
authors.initials: AR
authors.lastname: Silva
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D065468:Dasyproctidae; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004126:Dimethylformamide / Q000494:pharmacology; D004822:Epididymis / Q000187:drug effects; D019855:Ethylene Glycol / Q000494:pharmacology; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology; D008297:Male; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D004126:Dimethylformamide; D019855:Ethylene Glycol; D005990:Glycerol; D004121:Dimethyl Sulfoxide
keywords: Agouti epididymal sperm; Cryopreservation; Cryoprotectant; Dasyprocta aguti; Germplasm bank