title: Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording.
abstract: Superior cervical ganglion neurons (SCGN) are often used to investigate neurotransmitter release mechanisms. In this study, we optimized the dissociation and culture conditions of rat SCGN cultures for dual patch clamp recordings. Two weeks in vitro are sufficient to achieve a significant CNTF-induced cholinergic switch and to develop mature and healthy neuronal profiles suited for detailed patch clamp analysis. One single pup provides sufficient material to prepare what was formerly obtained from 12 to 15 animals. The suitability of these cultures to study neurotransmitter release mechanisms was validated by presynaptically perturbing the interaction of the v-SNARE VAMP2 with the vesicular V-ATPase V0c subunit.
authors.affiliation: INSERM, UMR_S 1072, Marseille, France.|Aix-Marseille Université, Marseille, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Amendola
authors.affiliation: INSERM, UMR_S 1072, Marseille, France.|Aix-Marseille Université, Marseille, France.
authors.forename: Norah
authors.initials: N
authors.lastname: Boumedine
authors.affiliation: INSERM, UMR_S 1072, Marseille, France.|Aix-Marseille Université, Marseille, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Sangiardi
authors.affiliation: INSERM, UMR_S 1072, Marseille, France.|Aix-Marseille Université, Marseille, France.
authors.forename: Oussama
authors.initials: O
authors.lastname: El Far
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D059329:Cholinergic Neurons / Q000187:drug effects / Q000502:physiology; D055724:Electrophysiological Phenomena; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000378:metabolism; D051381:Rats; D017783:Superior Cervical Ganglion / Q000166:cytology*; D009435:Synaptic Transmission
chemical_list: D009414:Nerve Growth Factors; D018377:Neurotransmitter Agents