title: Evaluation of the Role of Granzyme B in Exuberant Scar Pathogenesis: An Immunohistochemical Study.
abstract: OBJECTIVE
  To evaluate the possible role of Granzyme B (GzmB) in abnormal wound healing through its immunohistochemical expression in keloid and hypertrophic scars and to study the relationship of its expression with the clinicopathologic parameters of studied cases.
  
  
  METHODS
  Using immunohistochemical techniques, GzmB was analyzed in skin biopsies of 44 patients (30 cases with abnormal scars [21 with keloid and 9 with hypertrophic scars] and 14 age- and gender-matched cases with surgical scars as a control group).
  
  
  RESULTS
  GzmB was expressed in keratinocytes in 28.6%, 66.7%, and 23.8% of surgical scars, hypertrophic scars, and keloids, respectively. Dermal expression in inflammatory cells was detected in 64.3%, 44.4%, and 28.6% of surgical scars, hypertrophic scars, and keloids, respectively. No significant difference was noted in GzmB expression intensity or percent in comparison between studied groups.
  
  
  CONCLUSIONS
  GzmB plays no role in the pathogenesis of keloids and hypertrophic scars. Further large-scaled studies are needed to expand or refute the current observation.
authors.forename: Ola Ahmed
authors.initials: OA
authors.lastname: Bakry
authors.forename: Shereen Fouad
authors.initials: SF
authors.lastname: Younes
authors.forename: Moshira
authors.initials: M
authors.lastname: Abdel Wahed
authors.forename: Shawky Mahmoud
authors.initials: SM
authors.lastname: El Farargy
authors.forename: Shaimaa
authors.initials: S
authors.lastname: Sharawy
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002921:Cicatrix / Q000201:enzymology*; D005260:Female; D053804:Granzymes / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D014945:Wound Healing / Q000502:physiology; D055815:Young Adult
chemical_list: D053804:Granzymes