title: Sensitivity of Human Malignant Melanoma Cell Lines to Newcastle Disease Virus.
abstract: OBJECTIVE
  Virotherapy may be a promising alternative to chemotherapy of malignant melanoma. In clinical trials using strains of Newcastle disease virus (NDV), only a fraction of patients with cancer responded to virotherapy. In the present study, we tried to find a correlation between the susceptibility of human melanoma cell lines to NDV and growth factor signaling pathways.
  
  
  METHODS
  Using an ATP assay, cytotoxicity of an NDV strain (MTH-68/H) was tested in 13 human melanoma cell lines. The activation state of growth factor signaling pathways was studied by the analysis of key signaling proteins.
  
  
  RESULTS
  MTH-68/H was found to be cytotoxic in all melanoma cells tested, but the IC50 values varied significantly. No correlation between the IC50 values and the rate of extracellular signal-regulated kinase (ERK) and AKT phosphorylation and phosphatase and tensin homologue (PTEN) expression was found.
  
  
  CONCLUSIONS
  Susceptibility of tumor cells to NDV may be affected by alterations other than those of RAS/ERK and phosphatidylinositol 3-kinase (PI3K)/AKT signaling in uninfected cells.
authors.affiliation: Department of Medical Biology, University of Pécs Medical School, Pécs, Hungary Signal Transduction Research Group, János Szentágothai Research Centre, Pécs, Hungary.
authors.forename: Marianna
authors.initials: M
authors.lastname: Pap
authors.affiliation: Department of Medical Biology, University of Pécs Medical School, Pécs, Hungary Signal Transduction Research Group, János Szentágothai Research Centre, Pécs, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Bátor
authors.affiliation: Department of Medical Biology, University of Pécs Medical School, Pécs, Hungary Signal Transduction Research Group, János Szentágothai Research Centre, Pécs, Hungary jozsef.szeberenyi@aok.pte.hu.
authors.forename: József
authors.initials: J
authors.lastname: Szeberényi
mesh_terms: D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020128:Inhibitory Concentration 50; D008545:Melanoma / Q000473:pathology / Q000628:therapy*; D009522:Newcastle disease virus / Q000145:classification; D050130:Oncolytic Virotherapy; D050504:Oncolytic Viruses / Q000145:classification; D015398:Signal Transduction
keywords: Melanoma; Newcastle disease virus; oncolytic viruses; virotherapy