title: Antidepressant-like and anti-oxidative efficacy of Campsis grandiflora flower.
abstract: OBJECTIVE
  Our study aimed to investigate the antidepressant-like effect of ethyl acetate extract of the flowers of Campsis grandiflora (EFCG) in a mice model of chronic unpredictable mild stress (CUMS).
  
  
  METHODS
  HPLC-Q-TOF-MS was used to identify the chemical constituents of EFCG. The DPPH assay and ABTS radical-scavenging assay were performed to measure the antioxidant properties. The protective properties of EFCG against H2 O2 -induced oxidative damage were analysed in PC12 cells. The changes of behaviour profiles were investigated by using open-field test, sucrose preference test, forced swimming test (FST) and tail suspension test (TST). Brain tissue samples of mice were collected, and antioxidative measure levels were measured.
  
  
  RESULTS
  The result showed that EFCG had the most active anti-oxidative effect and the protective effect against H2 O2 oxidative injury in PC12 cells. Treatment with the EFCG significantly reduced the depressant-like severity and immobility period as compared with untreated CUMS mice in FST and TST. Moreover, EFCG significantly elevated the contents of superoxide dismutase, Glutathione Peroxidase and decreased the contents of Malonaldehyde (MDA) in mice brain.
  
  
  CONCLUSIONS
  Our study found first the antidepressant activity of the EFCG. The results suggested the therapeutic potential of EFCG for depressive disorder.
authors.affiliation: School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, China.|Department of Psychiatry, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.|Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang, China.|Department of Pharmacology, School of Medicine, Zhengzhou University, Zhengzhou, China.
authors.forename: Hai-Chuan
authors.initials: HC
authors.lastname: Yu
authors.affiliation: School of Pharmacy, Xinxiang Medical University, Xinxiang, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Psychology, Xinxiang Medical University, Xinxiang, China.|Department of Psychiatry, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.|Department of Pharmacology, School of Medicine, Zhengzhou University, Zhengzhou, China.
authors.forename: Hong-Xing
authors.initials: HX
authors.lastname: Zhang
authors.affiliation: Department of Psychiatry, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
authors.forename: Hai-San
authors.initials: HS
authors.lastname: Zhang
authors.affiliation: School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Qiao
authors.affiliation: School of Pharmacy, Xinxiang Medical University, Xinxiang, China.
authors.forename: Ji-Xia
authors.initials: JX
authors.lastname: Zhang
authors.affiliation: School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, China.
authors.forename: Gao-Li
authors.initials: GL
authors.lastname: Zhang
authors.affiliation: School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Sui
authors.affiliation: School of Pharmacy, Xinxiang Medical University, Xinxiang, China.
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Li
authors.affiliation: Department of Pharmacology, School of Medicine, Zhengzhou University, Zhengzhou, China.
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Zhang
authors.affiliation: Department of Psychiatry, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
authors.forename: Lu-Xian
authors.initials: LX
authors.lastname: Lv
mesh_terms: D000085:Acetates / Q000737:chemistry; D000818:Animals; D000928:Antidepressive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects*; D052160:Benzothiazoles / Q000737:chemistry; D029402:Bignoniaceae / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D035264:Flowers; D005518:Food Preferences / Q000187:drug effects; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013315:Stress, Psychological / Q000188:drug therapy* / Q000523:psychology; D013451:Sulfonic Acids / Q000737:chemistry; D013550:Swimming
chemical_list: D000085:Acetates; D000928:Antidepressive Agents; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C007650:ethyl acetate; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: anti-oxidation; campsis grandiflora; antidepression; crude extracts; medicinal plants