title: Concurrent visual diagnosis and susceptibility profiling of the first line drug against visceral leishmaniasis by plasmonic detection of PCR amplified genetic biomarker.
abstract: Visceral form of leishmaniasis (also known as Kala-azar) is a fatal neglected tropical disease affecting 95 countries worldwide. Recently, substantial proportion of resistance related treatment failure cases have been reported against its first line drug, sodium-antimony gluconate (SAG). We report an easy, fast, sensitive and cheap visual diagnosis and SAG susceptibility profiling for this disease based on recently recognized genetic biomarker and gold nanoparticle based plasmonic detection phenomenon. This is a non-gel, non-culture based detection technique, which can be used as simultaneous high throughput detection and SAG-susceptibility profiling in Leishmania endemic resource stringent countries.
authors.affiliation: Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Hajipur, E.P.I.P Campus, Bihar, India. Electronic address: ppb.mm@jcbose.ac.in.
authors.forename: Partha Pratim
authors.initials: PP
authors.lastname: Bose
authors.affiliation: Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Hajipur, E.P.I.P Campus, Bihar, India.
authors.forename: Prakash
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Hajipur, E.P.I.P Campus, Bihar, India.
authors.forename: Narendar
authors.initials: N
authors.lastname: Munagala
mesh_terms: D000967:Antimony Sodium Gluconate / Q000627:therapeutic use; D000981:Antiprotozoal Agents / Q000627:therapeutic use; D004198:Disease Susceptibility; D004351:Drug Resistance; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007893:Leishmania donovani / Q000235:genetics; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000188:drug therapy* / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000981:Antiprotozoal Agents; D005819:Genetic Markers; D000967:Antimony Sodium Gluconate
keywords: Drug susceptibility profiling; Genetic biomarker; Gold nanoparticle; Polymeric chain reaction; Visual detection