title: Generation of Two Biological Wound Dressings as a Potential Delivery System of Human Adipose-Derived Mesenchymal Stem Cells.
abstract: Human adipose-derived mesenchymal stem cells (hADMSCs) are believed to be potential key factors for starting the regenerative process after tissue injury. However, an efficient method of delivering these regenerative cells to an external wound site is still lacking. Human amnion and pig skin have long been used as skin wound dressings for the treatment of burns and other skin lesions. Herein, we present the generation of two constructs using these two biomaterials as effective scaffolds for the culture of hADMSCs. It was found that hADMSCs seeded onto radiosterilized human amnion and pig skin are viable and proliferate. These cells are able to migrate over these scaffolds as demonstrated by using time-lapse microscopy. In addition, the scaffolds induce hADMSCs to secrete interleukin-10, an important negative regulator of inflammation, and interleukin-1β, a proinflammatory protein. The interplay between these two proteins has been proven to be vital for a balanced restoration of all necessary tissues. Thus, radiosterilized human amnion and pig skin are likely suitable scaffolds for delivery of hADMSCs transplants that could promote tissue regeneration in skin injuries like patients with burn injuries.
authors.affiliation: From the *Instituto Nacional de Rehabilitación, Laboratorio de Biotecnología, Centro Nacional de Investigación y Atención de Quemados, Calz, México Xochimilco No. 289, Col. Arenal de Guadalupe, Mexico, D.F.; †Instituto Nacional de Investigaciones Nucleares, Banco de Tejidos Radioesterilizados, Carretera México-Toluca s/n, La Marquesa, 52750 Ocoyoacac, Mexico; and ‡Facultad de Medicina, Universidad Nacional Autónoma de México. Av. Universidad 3000, Coyoacán, Mexico, D.F.
authors.forename: Roberto
authors.initials: R
authors.lastname: Sánchez-Sánchez
authors.forename: Ana
authors.initials: A
authors.lastname: Brena-Molina
authors.forename: Valentín
authors.initials: V
authors.lastname: Martínez-López
authors.forename: Yaaziel
authors.initials: Y
authors.lastname: Melgarejo-Ramírez
authors.forename: Lenin
authors.initials: L
authors.lastname: Tamay de Dios
authors.forename: Ricardo
authors.initials: R
authors.lastname: Gómez-García
authors.forename: Ma de Lourdes
authors.initials: Mde L
authors.lastname: Reyes-Frías
authors.forename: Lourdes
authors.initials: L
authors.lastname: Rodríguez-Rodríguez
authors.forename: David
authors.initials: D
authors.lastname: Garciadiego-Cázares
authors.forename: Haydée
authors.initials: H
authors.lastname: Lugo-Martínez
authors.forename: Clemente
authors.initials: C
authors.lastname: Ibarra
authors.forename: María Esther
authors.initials: ME
authors.lastname: Martínez-Pardo
authors.forename: Cristina
authors.initials: C
authors.lastname: Velasquillo-Martínez
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000650:Amnion / Q000502:physiology*; D000818:Animals; D001458:Bandages; D001672:Biocompatible Materials; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis; D053583:Interleukin-1beta / Q000096:biosynthesis; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D012867:Skin / Q000378:metabolism*; D013552:Swine; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000502:physiology*; D014947:Wounds and Injuries / Q000628:therapy
chemical_list: D001672:Biocompatible Materials; D053583:Interleukin-1beta; D016753:Interleukin-10