title: Treatment with platelet lysate induces endothelial differentation of bone marrow mesenchymal stem cells under fluid shear stress.
abstract: By considering stem cell-based therapies as a new hope for the treatment of some tragic diseases, marrow stromal cells or marrow mesenchymal stem cells (MSCs) were considered as a suitable and safe multipotential cell source for this new therapeutic approach. For this purpose, many investigations have been performed on differentiation of MSCs toward specific cell lines to overcome the demand for providing the organ specific cells for cell therapy or preparation of engineered tissues. In the present study, differentiation of MSCs to endothelial cells (ECs) by mechanical and chemical stimulation was evaluated. Fluid shear stress (FSS) was used as mechanical inducer, while platelet lysate (PL) and estradiol (E) were used as chemical induction factors. MSCs were placed under FSS with different forces (2, 5 and 10dyn/cm(2)) for different periods (6, 12 and 24 hours). In some groups, PL and E were added to the culture media to evaluate their effect on expression of EC specific markers. This investigation revealed that FSS with low tension (2.5-5 dyn/cm(2)) for a long time (24 hours) or high tension (10 dyn/cm(2)) in short time (6 hours) in the presence of PL could differentiate MSCs toward ECs. The presence of PL was necessary for initiation of endothelial differentiation, and in the absence of PL, there was not any expression of CD34 and Cadherin5 (Cdh5) among cells. Adding E to the culture medium did not change the rate of endothelial differentiation under FSS. Generated endothelial progenitors could produce von Willebrand factor (vWF) after two weeks culture and also they formed tubular structures after culture on matrigel.
authors.affiliation: Shahid Sadoughi University of Medical Sciences, Department of Physiology, School of Medicine, Yazd, Iran ; Shahid Sadoughi University of Medical Sciences, Neurobiomedical Research Center, School of Medicine, Yazd, Iran ; Yazd Cardiovascular Research Center.
authors.forename: Farshad
authors.initials: F
authors.lastname: Homayouni Moghadam
authors.affiliation: Shahid Sadoughi University of Medical Sciences, Department of Physiology, School of Medicine, Yazd, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Tayebi
authors.affiliation: Shahid Sadoughi University of Medical Sciences, Department of Medicinal Chemistry, School of Pharmacy, Yazd, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Moradi
authors.affiliation: Shahid Sadoughi University of Medical Sciences, Department of Medicinal Chemistry, School of Pharmacy, Yazd, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Nadri
authors.affiliation: Shahid Sadoughi University of Medical Sciences, English Language Department, School of Medicine, Yazd, Iran.
authors.forename: Kazem
authors.initials: K
authors.lastname: Barzegar
authors.affiliation: Shahid Sadoughi University of Medical Sciences, Department of Medical Parasitology and Mycology, School of Medicine, Yazd, Iran.
authors.forename: Gilda
authors.initials: G
authors.lastname: Eslami
keywords: differentiation; endothelial cell; fluid shear stress; mesenchymal stem cell; platelet lysate