title: High Sensitivity and High Detection Specificity of Gold-Nanoparticle-Grafted Nanostructured Silicon Mass Spectrometry for Glucose Analysis.
abstract: Desorption/ionization on silicon (DIOS) is a high-performance matrix-free mass spectrometry (MS) analysis method that involves using silicon nanostructures as a matrix for MS desorption/ionization. In this study, gold nanoparticles grafted onto a nanostructured silicon (AuNPs-nSi) surface were demonstrated as a DIOS-MS analysis approach with high sensitivity and high detection specificity for glucose detection. A glucose sample deposited on the AuNPs-nSi surface was directly catalyzed to negatively charged gluconic acid molecules on a single AuNPs-nSi chip for MS analysis. The AuNPs-nSi surface was fabricated using two electroless deposition steps and one electroless etching step. The effects of the electroless fabrication parameters on the glucose detection efficiency were evaluated. Practical application of AuNPs-nSi MS glucose analysis in urine samples was also demonstrated in this study.
authors.affiliation: Department of Mechanical Engineering, National Central University , Taoyuan City 32001, Taiwan.
authors.forename: Chia-Wen
authors.initials: CW
authors.lastname: Tsao
authors.affiliation: Department of Mechanical Engineering, National Central University , Taoyuan City 32001, Taiwan.
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Yang
mesh_terms: D005947:Glucose / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D006046:Gold; D005947:Glucose; D012825:Silicon
keywords: desorption/ionization on silicon; glucose analysis; mass spectrometry; matrix-free laser desorption/ionization; nanostructured silicon