title: [Quality standard study on Entadae Semen].
abstract: OBJECTIVE
  To establish the quality standard of Entadae Semen, and provide scientific basis for its quality control.
  
  
  METHODS
  TLC and HPLC were used for qualitative identification and quantitative analysis of phaseoloidin and entadamide A-O-β-D-glucopyranoside in Entadae Semen. The test of water content, ash and ethanol-soluble extractives of Entadae Semen was carried out according to the methods recorded in appendix of Chinese Pharmacopeia (2010 edition).
  
  
  RESULTS
  The TLC was well separated with clear spots. The linear range of phaseoloidin was between 0.014 to 2.747 g x L(-1) (r = 0.999 6, n = 9) with an average recovery rate of 101.06% (RSD 0.90%, n = 6); the linear range of entadamide A-O-β-D-glucopyranoside was between 0.002 to 0.452 g x L(-1) (r = 0.999 7, n = 9) withan average recovery rate of 101.52% (RSD 1.09%, n = 6). The content of phaseoloidin in sample is between 5.12% to 9.24%, entadamide A-O-β-D-glucopyranoside is between 0.55% to 2.17%, alcohol-soluble extracts is between 30.9% to 45.2%, water is between 6.6% to 8.6%, and total ash is between 2.4% to 2.9%.
  
  
  CONCLUSIONS
  The established standard is acceptable for quality control of Entadae Semen.
authors.forename: Hui-ping
authors.initials: HP
authors.lastname: Shen
authors.forename: Meng-yue
authors.initials: MY
authors.lastname: Wang
authors.forename: Xiao-bo
authors.initials: XB
authors.lastname: Li
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D007887:Fabaceae / Q000737:chemistry*; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal