title: Purification, structure features and anti-atherosclerosis activity of a Laminaria japonica polysaccharide.
abstract: A homogeneous polysaccharide (LJP12) was isolated from Laminaria japonica by diethylaminoethyl-cellulose and Sephacryl S-500 chromatography, with a molecular weight of 2.31×10(6)Da. Monosaccharide analysis showed that LJP12 was mainly composed of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 1:0.17:1.54:2.64:0.18. For these monosaccharides, mannose was suggested to be 1,4-linked and 1,3,6-linked while glucose was linked by 1,6-glycosidic bond. The xylose, arabinose and galactose were suggested to be the terminal residues. To study the effects of LJP12 on protecting against atherosclerosis, LJP12 was administered to LDL receptor-deficient (LDLr(-/-)) mice (50, 100 and 200mg/kg/day, n=30 for each experimental group). Results showed that LJP12 exhibited the ability to inhibit high-fat-cholesterol diet (HFD)-induced formation of atherosclerotic plaques and plasma lipid levels in a dose-dependent manner. Meanwhile, both the HFD-induced systemic inflammation and local inflammation at the site of atherosclerotic lesion were significantly attenuated by LJP12, which were accompanied by the suppression of the activation of nuclear factor kappa-B (NF-κB) and mitogen-activated protein kinases (MAPKs) signaling pathways. Taken together, we concluded that long-term oral administration of LJP12 protects against atherosclerosis in LDLr(-/-) mice via inhibiting NF-κB/MAPKs-mediated inflammatory responses.
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, PR China.
authors.forename: Fu-Hua
authors.initials: FH
authors.lastname: Peng
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, PR China. Electronic address: zhaxueqiang@hfut.edu.cn.
authors.forename: Xue-Qiang
authors.initials: XQ
authors.lastname: Zha
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, PR China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Cui
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, PR China.
authors.forename: Muhammad-Naeem
authors.initials: MN
authors.lastname: Asghar
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, PR China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Pan
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, PR China.
authors.forename: Jun-Hui
authors.initials: JH
authors.lastname: Wang
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, PR China. Electronic address: jianpingluo@hfut.edu.cn.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Luo
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000097:blood / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D059168:Carotid Intima-Media Thickness; D015815:Cell Adhesion Molecules / Q000097:blood; D002465:Cell Movement / Q000187:drug effects; D018925:Chemokines / Q000097:blood; D059305:Diet, High-Fat; D005786:Gene Expression Regulation / Q000187:drug effects; D007795:Laminaria / Q000737:chemistry*; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D008055:Lipids / Q000097:blood; D008264:Macrophages / Q000187:drug effects / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008745:Methylation; D008810:Mice, Inbred C57BL; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009005:Monosaccharides / Q000032:analysis; D016328:NF-kappa B; D058226:Plaque, Atherosclerotic / Q000097:blood / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D015815:Cell Adhesion Molecules; D018925:Chemokines; D008055:Lipids; D009005:Monosaccharides; D016328:NF-kappa B; D011134:Polysaccharides; D012333:RNA, Messenger; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D020928:Mitogen-Activated Protein Kinases
keywords: Anti-atherosclerosis; Laminaria japonica; Polysaccharide