title: Uteroplacental insufficiency leads to hypertension, but not glucose intolerance or impaired skeletal muscle mitochondrial biogenesis, in 12-month-old rats.
abstract: Growth restriction impacts on offspring development and increases their risk of disease in adulthood which is exacerbated with "second hits." The aim of this study was to investigate if blood pressure, glucose tolerance, and skeletal muscle mitochondrial biogenesis were altered in 12-month-old male and female offspring with prenatal or postnatal growth restriction. Bilateral uterine vessel ligation induced uteroplacental insufficiency and growth restriction in offspring (Restricted). A sham surgery was also performed during pregnancy (Control) and some litters from sham mothers had their litter size reduced (Reduced litter), which restricted postnatal growth. Growth-restricted females only developed hypertension at 12 months, which was not observed in males. In Restricted females only homeostasis model assessment for insulin resistance was decreased, indicating enhanced hepatic insulin sensitivity, which was not observed in males. Plasma leptin was increased only in the Reduced males at 12 months compared to Control and Restricted males, which was not observed in females. Compared to Controls, leptin, ghrelin, and adiponectin were unaltered in the Restricted males and females, suggesting that at 12 months of age the reduction in body weight in the Restricted offspring is not a consequence of circulating adipokines. Skeletal muscle PGC-1α levels were unaltered in 12-month-old male and female rats, which indicate improvements in lean muscle mass by 12 months of age. In summary, sex strongly impacts the cardiometabolic effects of growth restriction in 12-month-old rats and it is females who are at particular risk of developing long-term hypertension following growth restriction.
authors.affiliation: Departments of Physiology, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Melanie
authors.initials: M
authors.lastname: Tran
authors.affiliation: Departments of Physiology, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Young
authors.affiliation: Departments of Physiology, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Jefferies
authors.affiliation: Departments of Physiology, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Deanne H
authors.initials: DH
authors.lastname: Hryciw
authors.affiliation: Departments of Anatomy and Neuroscience, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Ward
authors.affiliation: Departments of Anatomy and Neuroscience, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Erica L
authors.initials: EL
authors.lastname: Fletcher
authors.affiliation: Departments of Physiology, The University of Melbourne, Parkville, Victoria, Australia m.wlodek@unimelb.edu.au.
authors.forename: Mary E
authors.initials: ME
authors.lastname: Wlodek
authors.affiliation: Departments of Physiology, The University of Melbourne, Parkville, Victoria, Australia School of Exercise and Nutrition Sciences, Centre for Physical Activity and Nutrition Research, Deakin University, Burwood, Victoria, Australia.
authors.forename: Glenn D
authors.initials: GD
authors.lastname: Wadley
keywords: Aging; development; fetal programming