title: Ratiometric Fluorescent Polymeric Thermometer for Thermogenesis Investigation in Living Cells.
abstract: Intracellular temperature has a fundamental effect on cellular events. Herein, a novel fluorescent polymer ratiometric nanothermometer has been developed based on transferrin protein-stabilized gold nanoclusters as the targeting and fluorescent ratiometric unit and the thermosensitve polymer as the temperature sensing unit. The resultant nanothermometer could feature a high and spontaneous uptake into the HeLa cells and the ratiometric temperature sensing over the physiological temperature range. Moreover, the precise temperature sensing for intracellular heat generation in HeLa cells following calcium ions stress has been achieved. This practical intracellular thermometry could eliminate the interference of the intracellular surrounding environment in cancer cells without a microinjection procedure, which is user-friendly. The prepared new nanothermometer can provide tools for unveiling the intrinsic relationship between the intracellular temperature and ion channel function.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , No. 2 Zhongguancun Beiyijie, Beijing 100190, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Qiao
authors.affiliation: National Creative Research Center of Applied Microfluidic Chemistry, Department of Chemical Engineering, Pohang University of Science and Technology , Pohang, Gyeongbuk 790-784, Republic of Korea.
authors.forename: Yoon-Ho
authors.initials: YH
authors.lastname: Hwang
authors.affiliation: Beijing Key Laboratory of Bioelectromagnetism, Institute of Electrical Engineering , Beijing, 100190, People's Republic of China.
authors.forename: Chuan-Fang
authors.initials: CF
authors.lastname: Chen
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , No. 2 Zhongguancun Beiyijie, Beijing 100190, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Qi
authors.affiliation: Research Centre of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing, 100730, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , No. 2 Zhongguancun Beiyijie, Beijing 100190, People's Republic of China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Mu
authors.affiliation: National Creative Research Center of Applied Microfluidic Chemistry, Department of Chemical Engineering, Pohang University of Science and Technology , Pohang, Gyeongbuk 790-784, Republic of Korea.
authors.forename: Dong-Pyo
authors.initials: DP
authors.lastname: Kim
mesh_terms: D002468:Cell Physiological Phenomena; D002470:Cell Survival; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature; D022722:Thermogenesis; D013821:Thermometers
chemical_list: D011108:Polymers