title: Phylogenetic relationship of non-typeable Haemophilus influenzae isolated in Malaysia.
abstract: The epidemiology of non-typeable Haemophilus influenzae (NTHi) remains poorly understood. We therefore sought to determine the genetic relationship of 25 NTHi isolated from various states in Malaysia using multilocus sequence typing (MLST). The majority of isolates were obtained from sputum. There were 24 novel sequence types (STs). Eight isolates were single-locus variants, the remainder being singletons. Clustering was not based on clinical site of isolation or geographical origin. Despite the limited number of isolates examined in this study, we demonstrate that NTHi isolates in Malaysia are diverse and warrant further investigation.
authors.affiliation: Core of Health and Wellbeing, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia; Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh, Selangor, Malaysia. Electronic address: zainimz@salam.uitm.edu.my.
authors.forename: Zaini
authors.initials: Z
authors.lastname: Mohd-Zain
authors.affiliation: Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh, Selangor, Malaysia. Electronic address: mirakamsani@yahoo.com.
authors.forename: Nurul H
authors.initials: NH
authors.lastname: Kamsani
authors.affiliation: Institute for Medical Research, Kuala Lumpur, Malaysia. Electronic address: norazah@imr.gov.my.
authors.forename: Norazah
authors.initials: N
authors.lastname: Ahmad
authors.affiliation: Faculty of Medicine and Institute of Life Sciences, University of Southampton, UK; Southampton NIHR Respiratory Biomedical Research Unit, University Hospital Southampton Foundation NHS Trust, Southampton, UK; University of Southampton Malaysia Campus, Nusajaya, Johor, Malaysia. Electronic address: S.C.Clarke@soton.ac.uk.
authors.forename: Stuart C
authors.initials: SC
authors.lastname: Clarke
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006192:Haemophilus Infections / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008296:Malaysia; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial
keywords: MLST; Malaysia; Non-typeable Haemophilus influenzae; Phylogenetic