title: Self-Monitoring and Self-Delivery of Photosensitizer-Doped Nanoparticles for Highly Effective Combination Cancer Therapy in Vitro and in Vivo.
abstract: Theranostic nanomedicine is capable of diagnosis, therapy, and monitoring the delivery and distribution of drug molecules and has received growing interest. Herein, a self-monitored and self-delivered photosensitizer-doped FRET nanoparticle (NP) drug delivery system (DDS) is designed for this purpose. During preparation, a donor/acceptor pair of perylene and 5,10,15,20-tetro (4-pyridyl) porphyrin (H2TPyP) is co-doped into a chemotherapeutic anticancer drug curcumin (Cur) matrix. In the system, Cur works as a chemotherapeutic agent. In the meantime, the green fluorescence of Cur molecules is quenched (OFF) in the form of NPs and can be subsequently recovered (ON) upon release in tumor cells, which enables additional imaging and real-time self-monitoring capabilities. H2TPyP is employed as a photodynamic therapeutic drug, but it also emits efficient NIR fluorescence for diagnosis via FRET from perylene. By exploiting the emission characteristics of these two emitters, the combinatorial drugs provide a real-time dual-fluorescent imaging/tracking system in vitro and in vivo, and this has not been reported before in self-delivered DDS which simultaneously shows a high drug loading capacity (77.6%Cur). Overall, our carrier-free DDS is able to achieve chemotherapy (Cur), photodynamic therapy (H2TPyP), and real-time self-monitoring of the release and distribution of the nanomedicine (Cur and H2TPyP). More importantly, the as-prepared NPs show high cancer therapeutic efficiency both in vitro and in vivo. We expect that the present real-time self-monitored and self-delivered DDS with multiple-therapeutic and multiple-fluorescent ability will have broad applications in future cancer therapy.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica , Taipei, 115, Taiwan.
authors.forename: Yu-Chuan
authors.initials: YC
authors.lastname: Liang
authors.forename: Xudong
authors.initials: X
authors.lastname: Lin
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.affiliation: Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors.forename: Shengliang
authors.initials: S
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.forename: Guangyu
authors.initials: G
authors.lastname: Zhu
authors.forename: Andrey L
authors.initials: AL
authors.lastname: Rogach
authors.forename: Peter K N
authors.initials: PK
authors.lastname: Yu
authors.forename: Peng
authors.initials: P
authors.lastname: Shi
authors.affiliation: Department of Plastic Surgery, Mackay Memorial Hospital , Taipei, 10449, Taiwan.|Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan and Institute of Physics, Academia Sinica , Taipei, Taiwan.
authors.forename: Lung-Chen
authors.initials: LC
authors.lastname: Tu
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan and Institute of Physics, Academia Sinica , Taipei, Taiwan.
authors.forename: Chia-Ching
authors.initials: CC
authors.lastname: Chang
authors.affiliation: Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University , Suzhou, Jiangsu 215123, P. R. China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Forensic Sciences, Faculty of Life Sciences, University of Bradford , Bradford, BD7 1DP, U.K.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Chen
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
authors.forename: Chun-Sing
authors.initials: CS
authors.lastname: Lee
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use; D045744:Cell Line, Tumor; D003474:Curcumin / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D008297:Male; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000175:diagnosis* / Q000628:therapy*; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use; D000068936:Theranostic Nanomedicine; D015027:Zebrafish
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D017319:Photosensitizing Agents; D003474:Curcumin
keywords: FRET; combination therapy; in vitro; in vivo; self-delivery; self-monitoring