title: Prevalence and Virulence Characterization of Listeria monocytogenes in Chilled Pork in Zhejiang Province, China.
abstract: Listeria monocytogenes is an important foodborne pathogen that can grow in refrigeration temperature and causes severe human infections. The aims of this work were to estimate the prevalence of L. monocytogenes in chilled pork in Zhejiang, China and to examine the virulence features of the isolates. Of 331 meat samples, 196 were positive for Listeria spp., with L. innocua accounting for 54.4%, L. monocytogenes for 11.5%, and L. welshimeri for 4.2%. The most prevalent L. monocytogenes serotype was 1/2c (60.5%), followed by serotypes 1/2a (28.9%), 1/2b (7.9%), and 4b (2.6%). All L. monocytogenes isolates contained virulence-associated genes examined. Adhesion and invasion ability of serotype 1/2c isolates was much lower than those of other serotypes. Only one isolate was defective in cell-to-cell spread. These findings are important for risk assessment of chilled pork as a source of potential transmission of L. monocytogenes to other food products, particularly to ready-to-eat food products.
authors.affiliation: 1 Zhejiang University Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine , Hangzhou, Zhejiang, China .
authors.forename: Chun
authors.initials: C
authors.lastname: Fang
authors.affiliation: 1 Zhejiang University Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine , Hangzhou, Zhejiang, China .
authors.forename: Ying
authors.initials: Y
authors.lastname: Shan
authors.affiliation: 1 Zhejiang University Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine , Hangzhou, Zhejiang, China .
authors.forename: Tong
authors.initials: T
authors.lastname: Cao
authors.affiliation: 1 Zhejiang University Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine , Hangzhou, Zhejiang, China .
authors.forename: Ye
authors.initials: Y
authors.lastname: Xia
authors.affiliation: 1 Zhejiang University Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine , Hangzhou, Zhejiang, China .
authors.forename: Yongping
authors.initials: Y
authors.lastname: Xin
authors.affiliation: 2 College of Animal Science and Technology, Zhejiang A&F University , Lin'an, Zhejiang, China .
authors.forename: Changyong
authors.initials: C
authors.lastname: Cheng
authors.affiliation: 2 College of Animal Science and Technology, Zhejiang A&F University , Lin'an, Zhejiang, China .
authors.forename: Houhui
authors.initials: H
authors.lastname: Song
authors.affiliation: 1 Zhejiang University Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine , Hangzhou, Zhejiang, China .
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Li
authors.affiliation: 1 Zhejiang University Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine , Hangzhou, Zhejiang, China .|2 College of Animal Science and Technology, Zhejiang A&F University , Lin'an, Zhejiang, China .
authors.forename: Weihuan
authors.initials: W
authors.lastname: Fang
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D003080:Cold Temperature; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000276:immunology / Q000472:pathogenicity; D008088:Listeriosis / Q000453:epidemiology / Q000382:microbiology*; D058889:Molecular Typing; D010641:Phenotype; D015995:Prevalence; D000069466:Red Meat / Q000382:microbiology*; D012703:Serotyping; D013552:Swine; D014774:Virulence