title: Detection of Short-Range DNA Interactions in Mammalian Cells Using High-Resolution Circular Chromosome Conformation Capture Coupled to Deep Sequencing.
abstract: DNA interactions shape the genome to physically and functionally connect regulatory elements to their target genes. Studying these interactions is crucial to understanding the molecular mechanisms that regulate gene expression. In this chapter, we present a protocol for high-resolution circular chromosome conformation capture coupled to deep sequencing. This methodology allows to investigate short-range DNA interactions (<100 kbp) and to obtain high-resolution DNA interaction maps of loci. It is a powerful tool to explore how regulatory elements and genes are connected together.
authors.affiliation: Département de Biologie, Faculté des Sciences, Université de Sherbrooke, 2500 Boulevard de L'Université, Sherbrooke, QC, Canada, J1K 2R1. Jean-Francois.Millau@usherbrooke.ca.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Millau
authors.affiliation: Département de Biologie, Faculté des Sciences, Université de Sherbrooke, 2500 Boulevard de L'Université, Sherbrooke, QC, Canada, J1K 2R1.
authors.forename: Luc
authors.initials: L
authors.lastname: Gaudreau
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D002875:Chromosomes / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D009690:Nucleic Acid Conformation; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*
chemical_list: D002843:Chromatin; D004247:DNA
keywords: 4C; Circular chromosome conformation capture; Short-range interactions