title: Different Expression of Hypoxic and Angiogenic Factors in Human Endometriotic Lesions.
abstract: Endometriosis is associated with local angiogenic and hypoxic mechanisms. Indeed, peritoneal fluid of women with endometriosis generates a specific microenvironment to support the growth and development of ectopic endometrial tissues. The association between proangiogenic markers and hypoxic processes in different endometriosis phenotypes was investigated in the present study, analyzing the expression of several genes, related to hypoxic signaling pathway and involved in angiogenic processes, in nonpregnant women with different forms of endometriosis. Samples of ovarian endometrioma (OMA; n = 16) or deep infiltrating endometriosis (DIE; n = 11) were collected, and in addition, control endometrium was collected from healthy women by hysteroscopy. The gene expression of the hypoxia-inducible factors (HIF) 1/2α, protease-activated receptors (PARs) ¼, and vascular endothelial growth factor (VEGF) A was evaluated by quantitative reverse-transcription polymerase chain reaction. Ovarian endometrioma expresses high levels of HIF-1/2α, PAR-1/4, and VEGF-A, while DIE did not show significantly different gene expression compared to endometrium from unaffected women. A positive correlation between the expression of HIF-1/2α and VEGF-A mRNA was observed in OMA. The overall data point out that the heterogeneity of the disease reflects differences in expression levels of genes associated with hypoxia and angiogenesis, suggesting that such conditions may have an active role in the development of the disease.
authors.affiliation: Cellular and Molecular Physiology Unit, Department of Molecular and Developmental Medicine, University of Siena, Siena, Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Filippi
authors.affiliation: Obstetrics and Gynecology Unit, Department of Molecular and Developmental Medicine, "S. Maria alle Scotte" Hospital, University of Siena, Siena, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Carrarelli
authors.affiliation: Obstetrics and Gynecology Unit, Department of Molecular and Developmental Medicine, "S. Maria alle Scotte" Hospital, University of Siena, Siena, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Luisi
authors.affiliation: Department of Immunology, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, Paris, France Université Paris Descartes, Sorbonne Paris Cité, Inserm, Unité de Recherche, Institut Cochin, CNRS, Paris, France.
authors.forename: Frederic
authors.initials: F
authors.lastname: Batteux
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, Inserm, Unité de Recherche, Institut Cochin, CNRS, Paris, France Department of Gynecology Obstetrics II and Reproductive Medicine, Université Paris Descartes, Sorbone Paris Cité, Faculté de Médecine, Assistance Publique-Hôpitaux de Paris, Groupe Hospitalier Universitaire Ouest, Centre Hospitalier Universitaire Cochin, Paris, France.
authors.forename: Charles
authors.initials: C
authors.lastname: Chapron
authors.affiliation: Cellular and Molecular Physiology Unit, Department of Molecular and Developmental Medicine, University of Siena, Siena, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Naldini
authors.affiliation: Obstetrics and Gynecology Unit, Department of Molecular and Developmental Medicine, "S. Maria alle Scotte" Hospital, University of Siena, Siena, Italy felice.petraglia@unisi.it.
authors.forename: Felice
authors.initials: F
authors.lastname: Petraglia
mesh_terms: D000328:Adult; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis* / Q000235:genetics; D004715:Endometriosis / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis* / Q000235:genetics; D009389:Neovascularization, Pathologic / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D018179:Receptors, Thrombin / Q000096:biosynthesis / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics; D055815:Young Adult; D000099162:Endothelial PAS Domain-Containing Protein 1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D018179:Receptors, Thrombin; D042461:Vascular Endothelial Growth Factor A; D000099162:Endothelial PAS Domain-Containing Protein 1; C497442:HIF1A protein, human; C467484:VEGFA protein, human; C112908:protease-activated receptor 4
keywords: deep infiltrating endometriosis; hypoxia-inducible factors; ovarian endometrioma; protease-activated receptors; vascular endothelial growth factor