title: DNA damaging and biochemical effects of potassium tetraborate.
abstract: Potassium tetraborate (PTB) is a product resulting from the controlled reaction of potassium hydroxide, water and boric acid (BA). It is used in many areas of industry such as disinfectant, detergent and treatment of contact lenses. PTB is one of the boron compounds which is most commonly used in many areas of industry although very limited information is available concerning its toxicity. Therefore, in this study, it is aimed to determine genetic and biochemical effects of PTB in human blood cell cultures (n=4). PTB was added into culture tubes at various concentrations (0-1280 µg/ml). Micronucleus (MN) and chromosomal aberration (CA) tests were performed for genotoxic damage influences estimation. In addition, biochemical parameters (total antioxidant capacity (TAC) and total oxidative status (TOS) were examined to determine oxidative effects. The results indicated that all tested concentrations of PTB were found to be non-genotoxic. In addition, low concentrations (1.25, 2.5 and 5 µg/ml) of PTB caused increases of TAC levels. Furthermore, all concentrations of PTB were not changed the TOS levels in cultured human blood cells. Based on these results, in this study it has been reported for the first time that PTB is not genotoxic and it increases the antioxidant capacity in human peripheral blood lymphocytes.
authors.affiliation: Department of Chemistry, Science and Letter Faculty, Bitlis Eren University, Turkey.
authors.forename: Fatih Çaglar
authors.initials: FÇ
authors.lastname: Çelikezen
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Turkez
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, Turkey.
authors.forename: Basak
authors.initials: B
authors.lastname: Togar
authors.affiliation: Department of Chemistry, Science and Letter Faculty, Bitlis Eren University, Turkey.
authors.forename: Mehmet Sait
authors.initials: MS
authors.lastname: Izgi
keywords: antioxidant; boron; genotoxicity; oxidative stress; potassium tetraborate