title: Fast 3-D temporal focusing microscopy using an electrically tunable lens.
abstract: In this paper, we present a 3-D temporal focusing microscope based on an electrically tunable lens (ETL) and a femtosecond regenerative laser amplifier. The focus-tunable lens provides a fast and compact way to perform non-mechanical z-scanning and resolves the blurry image issue compared with GVD-based z-scanning methods. The optical performance of the temporal focusing system, including z-scanning characteristics, the associated the magnification variation, and the lateral and axial resolution, has been studied and characterized using calibrated Rhodamine-6G thin film sample, fluorescent beads, and pollen samples. Lastly, we demonstrate the optical cross-sectioning and z-scanning capability with an in vivo experiment, where Ca(2+) imaging of neurons in GaCamp6 labeled zebrafish was performed.
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.forename: Dapeng
authors.initials: D
authors.lastname: Zhang
authors.forename: Steven
authors.initials: S
authors.lastname: Walker
authors.forename: Chenglin
authors.initials: C
authors.lastname: Gu
authors.forename: Ya
authors.initials: Y
authors.lastname: Ke
authors.forename: Wing Ho
authors.initials: WH
authors.lastname: Yung
authors.forename: Shih-chi
authors.initials: SC
authors.lastname: Chen