title: Stabilizing proteins for affinity capillary electrophoresis using ionic liquid aqueous two phase systems: Pharmaceuticals and human serum albumin.
abstract: Recently, ionic liquids (ILs) are finding ever broader scope within pharmaceutical and bioanalytical applications. In the current work, ACE binding measurements of tryptophan (Try)-HSA, chlorambucil (CHL)-HSA, and dacarbazine (DTIC)-HSA complexes were estimated in the absence or presence of several short chain imidazolium ILs within the range of concentrations of 10.0-1000.0 μmol/L that are far below the critical micelle concentrations of ILs. Results indicated that the value of binding constant of Trp-HSA was dramatically deviated in the presence of 1000.0 μmol/L 1-decyl-3-methylimidazolium bromide (DMIMBr) IL. However, interestingly, there is no any deviation for the Trp-HSA binding constant with 100.0 μmol/L 1-butyl-3-methylimidazolium bromide (BMIMBr) IL as an adjuvant additive in 67.0 mmol/L phosphate buffer at pH 7.4. This finding was further used to estimate the binding constants of important but weakly binding substances of CHL and DTIC antitumors with HSA; their binding constants were also estimated by HPAC giving data in good agreement with that revealed by ACE. These achievements were attributed to the significant improvement of HSA stability by combination with BMIMBr IL through hydrogen bond, electrostatic, and π-π forces. In addition, the use of 100.0 μmol/L BMIMBr extended the stability of native HSA solution stored under the ambient lab conditions up to 25 days with significant improvements in the precision of ACE binding data.
authors.affiliation: Chemistry Department, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia.|Chemistry Department, Faculty of Science, Assiut University, Assiut, Egypt.
authors.forename: Deia Abd
authors.initials: DA
authors.lastname: El-Hady
authors.affiliation: Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Hassan M
authors.initials: HM
authors.lastname: Albishri
authors.affiliation: Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Rengarajan
authors.affiliation: Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Braunschweig, Germany.
authors.forename: Sami
authors.initials: S
authors.lastname: El Deeb
authors.affiliation: Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Braunschweig, Germany.
authors.forename: Hermann
authors.initials: H
authors.lastname: Wätzig
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D055550:Protein Stability; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D007093:Imidazoles; D052578:Ionic Liquids; D004364:Pharmaceutical Preparations; D012709:Serum Albumin
keywords: Affinity capillary electrophoresis; Binding constants; Ionic liquids; Pharmaceuticals; Protein stability