title: Spiribacter curvatus sp. nov., a moderately halophilic bacterium isolated from a saltern.
abstract: A novel pink-pigmented bacterial strain, UAH-SP71T, was isolated from a saltern located in Santa Pola, Alicante (Spain) and the complete genome sequence was analysed and compared with that of Spiribacter salinus M19-40T, suggesting that the two strains constituted two separate species, with a 77.3% ANI value. In this paper, strain UAH-SP71T was investigated in a taxonomic study using a polyphasic approach. Strain UAH-SP71T was a Gram-stain-negative, strictly aerobic, non-motile curved rod that grew in media containing 5-20% (w/v) NaCl (optimum 10% NaCl), at 5-40 °C (optimum 37 °C) and at pH 5-10 (optimum pH 8). Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed thatstrain UAH-SP71T is a member of the genus Spiribacter, showing a sequence similarity of 96.5% with Spiribacter salinus M19-40T. Other related species are also members of the family Ectothiorhodospiraceae, including Arhodomonas recens RS91T (95.5% 16S rRNA gene sequence similarity), Arhodomonas aquaeolei ATCC 49307T (95.4 %) and Alkalilimnicola ehrlichii MLHE-1T (94.9 %). DNA-DNA hybridization between strain UAH-SP71T and Spiribacter salinus M19-40T was 39 %. The major cellular fatty acids of strain UAH-SP71T were C18 : 1ω6c and/or C18 : 1ω7c, C16 : 0, C16 : 1ω6c and/or C16 : 1ω7c, C10 : 0 3-OH and C12 : 0, a pattern similar to that of Spiribacter salinus M19-40T. Phylogenetic, phenotypic and genotypic differences between strain UAH-SP71T and Spiribacter salinus M19-40T indicate that strainUAH-SP71T represents a novel species of the genus Spiribacter, for which the name Spiribacter curvatus sp. nov. is proposed. The type strain is UAH-SP71T (5CECT8396T5DSM 28542T).
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors.forename: María José
authors.initials: MJ
authors.lastname: León
authors.affiliation: Department of Biomedicine and Biotechnology, Faculty of Pharmacy, University of Alcalá, Alcalá de Henares, 28871 Madrid, Spain.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Rodríguez-Olmos
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Sánchez-Porro
authors.affiliation: Evolutionary Genomics Group, Microbiology Division, University Miguel Hernández, San Juan, 03550 Alicante, Spain.
authors.forename: Mario
authors.initials: M
authors.lastname: López-Pérez
authors.affiliation: Evolutionary Genomics Group, Microbiology Division, University Miguel Hernández, San Juan, 03550 Alicante, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Rodríguez-Valera
authors.affiliation: Department of Biomedicine and Biotechnology, Faculty of Pharmacy, University of Alcalá, Alcalá de Henares, 28871 Madrid, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Soliveri
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Ventosa
authors.affiliation: Department of Biomedicine and Biotechnology, Faculty of Pharmacy, University of Alcalá, Alcalá de Henares, 28871 Madrid, Spain.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Copa-Patiño
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D042001:Ectothiorhodospiraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D013030:Spain; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S