title: New functionally-enhanced soy proteins as food ingredients with anti-viral activity.
abstract: Respiratory viruses are a major public health problem because of their prevalence and high morbidity rate leading to considerable social and economic implications. Cranberry has therapeutic potential attributed to a comprehensive list of phytochemicals including anthocyanins, flavonols, and unique A-type proanthocyanidins. Soy flavonoids, including isoflavones, have demonstrated anti-viral effects in vitro and in vivo. Recently, it was demonstrated that edible proteins can efficiently sorb and concentrate cranberry polyphenols, including anthocyanins and proanthocyanins, providing greatly stabilized matrices suitable for food products. The combination of cranberry and soy phytoactives may be an effective dietary anti-viral resource. Anti-viral properties of both cranberry juice-enriched and cranberry pomace polyphenol-enriched soy protein isolate (CB-SPI and CBP-SPI) were tested against influenza viruses (H7N1, H5N3, H3N2), Newcastle disease virus and Sendai virus in vitro and in ovo. In our experiments, preincubation with CB-SPI or CBP-SPI resulted in inhibition of virus adsorption to chicken red blood cells and reduction in virus nucleic acid content up to 16-fold, however, CB-SPI and CBP-SPI did not affect hemagglutination. Additionally, CB-SPI and CBP-SPI inhibited viral replication and infectivity more effectively than the commercially available anti-viral drug Amizon. Results suggest CB-SPI and CBP-SPI may have preventative and therapeutic potential against viral infections that cause diseases of the respiratory and gastro-intestinal tract.
authors.affiliation: Institute of Microbiology and Virology, 103, Bogenbai Batyr Street, Almaty, 050010 Kazakhstan.
authors.forename: Aizhan Sabirzhanovna
authors.initials: AS
authors.lastname: Turmagambetova
authors.affiliation: Institute of Microbiology and Virology, 103, Bogenbai Batyr Street, Almaty, 050010 Kazakhstan.
authors.forename: Nadezhda Sergeevna
authors.initials: NS
authors.lastname: Sokolova
authors.affiliation: Institute of Microbiology and Virology, 103, Bogenbai Batyr Street, Almaty, 050010 Kazakhstan.
authors.forename: Andrey Pavlinovich
authors.initials: AP
authors.lastname: Bogoyavlenskiy
authors.affiliation: Institute of Microbiology and Virology, 103, Bogenbai Batyr Street, Almaty, 050010 Kazakhstan.
authors.forename: Vladimir Eleazarovich
authors.initials: VE
authors.lastname: Berezin
authors.affiliation: Plants for Human Health Institute, North Carolina State University, 600 Laureate Way, Kannapolis, NC 28081 USA.
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Lila
authors.affiliation: Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, 59 Dudley Road, New Brunswick, NJ 08901 USA.
authors.forename: Diana M
authors.initials: DM
authors.lastname: Cheng
authors.affiliation: Hostos Community College, City University of New York, 500 Grand Concourse, Bronx, NY 10451 USA.
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Dushenkov
keywords: Anti-viral; Cranberry; Flavonoids; Polyphenols