title: Large-scale live imaging of adult neural stem cells in their endogenous niche.
abstract: Live imaging of adult neural stem cells (aNSCs) in vivo is a technical challenge in the vertebrate brain. Here, we achieve long-term imaging of the adult zebrafish telencephalic neurogenic niche and track a population of >1000 aNSCs over weeks, by taking advantage of fish transparency at near-infrared wavelengths and of intrinsic multiphoton landmarks. This methodology enables us to describe the frequency, distribution and modes of aNSCs divisions across the entire germinal zone of the adult pallium, and to highlight regional differences in these parameters.
authors.affiliation: Paris-Saclay Institute for Neuroscience, CNRS UMR9197 - Université Paris-Sud, Team Zebrafish Neurogenetics, Avenue de la Terrasse, Building 5, Gif-sur-Yvette F-91198, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Dray
authors.affiliation: Paris-Saclay Institute for Neuroscience, CNRS UMR9197 - Université Paris-Sud, Team Zebrafish Neurogenetics, Avenue de la Terrasse, Building 5, Gif-sur-Yvette F-91198, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Bedu
authors.affiliation: Laboratory for Optics and Biosciences, École Polytechnique, Centre National de la Recherche Scientifique (UMR 7645) and Institut National de la Santé et de la Recherche Médicale (U1182), Palaiseau 91128, France.
authors.forename: Nelly
authors.initials: N
authors.lastname: Vuillemin
authors.affiliation: Paris-Saclay Institute for Neuroscience, CNRS UMR9197 - Université Paris-Sud, Team Zebrafish Neurogenetics, Avenue de la Terrasse, Building 5, Gif-sur-Yvette F-91198, France.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Alunni
authors.affiliation: Paris-Saclay Institute for Neuroscience, CNRS UMR9197 - Université Paris-Sud, Team Zebrafish Neurogenetics, Avenue de la Terrasse, Building 5, Gif-sur-Yvette F-91198, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Coolen
authors.affiliation: Paris-Saclay Institute for Neuroscience, CNRS UMR9197 - Université Paris-Sud, Team Zebrafish Neurogenetics, Avenue de la Terrasse, Building 5, Gif-sur-Yvette F-91198, France.
authors.forename: Monika
authors.initials: M
authors.lastname: Krecsmarik
authors.affiliation: Laboratory for Optics and Biosciences, École Polytechnique, Centre National de la Recherche Scientifique (UMR 7645) and Institut National de la Santé et de la Recherche Médicale (U1182), Palaiseau 91128, France.
authors.forename: Willy
authors.initials: W
authors.lastname: Supatto
authors.affiliation: Laboratory for Optics and Biosciences, École Polytechnique, Centre National de la Recherche Scientifique (UMR 7645) and Institut National de la Santé et de la Recherche Médicale (U1182), Palaiseau 91128, France emmanuel.beaurepaire@polytechnique.edu bally-cuif@inaf.cnrs-gif.fr.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Beaurepaire
authors.affiliation: Paris-Saclay Institute for Neuroscience, CNRS UMR9197 - Université Paris-Sud, Team Zebrafish Neurogenetics, Avenue de la Terrasse, Building 5, Gif-sur-Yvette F-91198, France emmanuel.beaurepaire@polytechnique.edu bally-cuif@inaf.cnrs-gif.fr.
authors.forename: Laure
authors.initials: L
authors.lastname: Bally-Cuif
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000378:metabolism*; D019070:Cell Lineage; D049109:Cell Proliferation; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008164:Luminescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D058953:Neural Stem Cells / Q000166:cytology*; D059906:Neuroimaging / Q000379:methods*; D055153:Stem Cell Niche; D013687:Telencephalon; D013696:Temperature; D019076:Transgenes; D015027:Zebrafish; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein
keywords: Live imaging; Multiphoton microscopy; Neural stem cell; Pallium; SHG/THG; Zebrafish