title: Fitting the Pieces of the β-Barrel Assembly Machinery Complex.
abstract: β-Barrel membrane proteins are found in the outer membranes of mitochondria, chloroplasts, and Gram-negative bacteria; however, exactly how they are folded and inserted remains unknown. Over the past decade, both functional and structural studies have greatly contributed to addressing this elusive mechanism. It is known that a conserved core machinery is required for each organelle, though the overall composition varies significantly. The vast majority of studies that aimed to understand the biogenesis of β-barrel membrane proteins has been conducted in Gram-negative bacteria. Here, it is the task of a multicomponent complex known as the β-barrel assembly machinery (BAM) complex to fold and insert new β-barrel membrane proteins into the outer membrane. In this review, we will discuss recent discoveries with the goal of utilizing all reported structural and functional studies to piece together a current structural model for the fully assembled BAM complex.
authors.affiliation: Markey Center for Structural Biology, Department of Biological Sciences, Purdue University , West Lafayette, Indiana 47907, United States.
authors.forename: Patrick K
authors.initials: PK
authors.lastname: O'Neil
authors.affiliation: National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health , Bethesda, Maryland 20892, United States.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Rollauer
authors.affiliation: Markey Center for Structural Biology, Department of Biological Sciences, Purdue University , West Lafayette, Indiana 47907, United States.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Noinaj
authors.affiliation: National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health , Bethesda, Maryland 20892, United States.
authors.forename: Susan K
authors.initials: SK
authors.lastname: Buchanan
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D006090:Gram-Negative Bacteria / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D008958:Models, Molecular; D060066:Protein Interaction Maps; D017433:Protein Structure, Secondary
chemical_list: D001425:Bacterial Outer Membrane Proteins