title: Cyclodextrin-modified MEKC method for quantification of selected acidic metabolites of catecholamines in the presence of various biogenic amines. Application to diagnosis of neuroblastoma.
abstract: The main aim of the presented study was to develop a reliable and non-time-consuming method for the simultaneous separation of biogenic amines (BAs) like noradrenalin, adrenalin, dopamine and their main metabolites - homovanillic acid (HVA), vanillylmandelic acid (VMA), 3,4-dihydroxyphenylacetic acid (DOPAC) - in urine samples. To achieve this, the validated α-cyclodextrin (α-CD)-modified micellar electrokinetic chromatography method with DAD was proposed. The optimized separation parameters were as follows: background electrolyte was composed of 10mM sodium tetraborate decahydrate, 30mM SDS, 15% (v/v) methanol and 25mM α-CD, adjusted to pH 9.36 with 1N NaOH; uncoated fused silica capillary (75μm i.d.×60.2cm length); λ=200nm; injection time 5s, applied voltage 25kV; temperature 25 (±0.1)°C. Next, the developed MEKC method was practically applied to evaluate the levels of selected acidic metabolites of catecholamines like HVA, VMA and DOPAC in urine samples collected from patients diagnosed with neuroblastoma (NB), melanotic neuroectodermal tumor of infancy (MNTI).
authors.affiliation: Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Miękus
authors.affiliation: Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Kowalski
authors.affiliation: Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Ilona
authors.initials: I
authors.lastname: Olędzka
authors.affiliation: Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Alina
authors.initials: A
authors.lastname: Plenis
authors.affiliation: Department of Pediatrics, Hematology and Oncology, Medical University of Gdańsk, Dębinki 7, 80-211 Gdańsk, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Bień
authors.affiliation: Department of Pediatrics, Hematology and Oncology, Medical University of Gdańsk, Dębinki 7, 80-211 Gdańsk, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Miękus
authors.affiliation: Department of Pediatrics, Hematology and Oncology, Medical University of Gdańsk, Dębinki 7, 80-211 Gdańsk, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Krawczyk
authors.affiliation: Department of Pediatrics, Hematology and Oncology, Medical University of Gdańsk, Dębinki 7, 80-211 Gdańsk, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Adamkiewicz-Drożyńska
authors.affiliation: Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland. Electronic address: tbaczek@gumed.edu.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Bączek
mesh_terms: D000293:Adolescent; D001679:Biogenic Amines / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009447:Neuroblastoma / Q000175:diagnosis* / Q000652:urine
chemical_list: D001679:Biogenic Amines; D002395:Catecholamines; D003505:Cyclodextrins
keywords: Biogenic amines; Biomarkers; Micellar electrokinetic chromatography technique; Neuroblastoma; Urine sample pre-treatment