title: Titanium Dioxide Nanoparticles (TiO₂) Quenching Based Aptasensing Platform: Application to Ochratoxin A Detection.
abstract: We demonstrate for the first time, the development of titanium dioxide nanoparticles (TiO₂) quenching based aptasensing platform for detection of target molecules. TiO₂ quench the fluorescence of FAM-labeled aptamer (fluorescein labeled aptamer) upon the non-covalent adsorption of fluorescent labeled aptamer on TiO₂ surface. When OTA interacts with the aptamer, it induced aptamer G-quadruplex complex formation, weakens the interaction between FAM-labeled aptamer and TiO₂, resulting in fluorescence recovery. As a proof of concept, an assay was employed for detection of Ochratoxin A (OTA). At optimized experimental condition, the obtained limit of detection (LOD) was 1.5 nM with a good linearity in the range 1.5 nM to 1.0 µM for OTA. The obtained results showed the high selectivity of assay towards OTA without interference to structurally similar analogue Ochratoxin B (OTB). The developed aptamer assay was evaluated for detection of OTA in beer sample and recoveries were recorded in the range from 94.30%-99.20%. Analytical figures of the merits of the developed aptasensing platform confirmed its applicability to real samples analysis. However, this is a generic aptasensing platform and can be extended for detection of other toxins or target analyte.
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France. sgbhand@gmail.com.|Biosensor Lab, Department of Chemistry, BITS, Pilani- K. K. Birla Goa Campus, Zuarinagar, Goa 403726, India. sgbhand@gmail.com.
authors.forename: Atul
authors.initials: A
authors.lastname: Sharma
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France. akhtarloona@gmail.com.|Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology (CIIT), Lahore 54000, Pakistan. akhtarloona@gmail.com.
authors.forename: Akhtar
authors.initials: A
authors.lastname: Hayat
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France. rupeshmishra02@gmail.com.|Department of Biosciences and Biotechnology, Banasthali University, Rajasthan 304022, India. rupeshmishra02@gmail.com.
authors.forename: Rupesh K
authors.initials: RK
authors.lastname: Mishra
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France. gaelle.catanante@univ-perp.fr.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Catanante
authors.affiliation: Biosensor Lab, Department of Chemistry, BITS, Pilani- K. K. Birla Goa Campus, Zuarinagar, Goa 403726, India. sgbhand@gmail.com.
authors.forename: Sunil
authors.initials: S
authors.lastname: Bhand
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France. jlmarty@univ-perp.fr.
authors.forename: Jean Louis
authors.initials: JL
authors.lastname: Marty
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D001515:Beer / Q000032:analysis / Q000382:microbiology; D015374:Biosensing Techniques / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D009793:Ochratoxins / Q000032:analysis*; D052156:SELEX Aptamer Technique / Q000379:methods*; D012680:Sensitivity and Specificity; D014025:Titanium / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D009793:Ochratoxins; C009495:titanium dioxide; C025589:ochratoxin A; D014025:Titanium; C045354:ochratoxin B
keywords: aptamer assay; beer; fluorescently labeled aptamer; ochratoxin A; quenching; titanium dioxide nanoparticles