title: Screening the Drug Resistance Property Among Aerobic Pathogenic Microorganisms of Dental Caries in North-Western Indian Population: A Preliminary Study.
abstract: OBJECTIVE
  To evaluate the emerging drug resistance among the caries pathogens isolated from carious dentine microbiologically.
  
  
  METHODS
  Specimens from dental caries were collected from 75 patients referred to Department of Conservative Dentistry and Endodontics, Vyas Dental College. Microbiological processing of all the samples was done within three hours to isolate the caries pathogens. The samples were inoculated on agar medium (Nutrient agar, Mac-conkey's agar) at 370C for 48 hours aerobically. The identification of strains was done by observing colony morphology and gram's staining. The predominant isolates were subjected to antimicrobial sensitivity test (Kirby Bauer's method). Statistical analysis of the isolates was done using paired t-test.
  
  
  RESULTS
  Out of 75 patients more common isolates were Staphylococcus aureus, Klebsiella, Pseudomonas aeruginosa, Yeast. The predominant were Staphylococcus and Pseudomonas species. Newer antibiotics were proved to be effective against these predominant strains after evaluating antibiotic sensitivity tests.
  
  
  CONCLUSIONS
  Although Streptococcus mutans (S.mutans) is the most prevalent microorganism seen in dental caries, the role of other microorganisms like Staphylococcus, Pseudomonas in initiation and progression of caries is evident from this study. Further extensive and large scale studies need to be conducted for better understanding the role of these microorganisms in dental caries.
authors.affiliation: PG student, Department of Oral & Maxillofacial Pathology, Vyas Dental College and Hospital , Pali road, near Kudi Haud, Jodhpur, India .
authors.forename: Navneet
authors.initials: N
authors.lastname: Kaur
authors.affiliation: Professor & HOD, Department of Oral & Maxillofacial Pathology, Narsinbhai Patel Dental College and Hospital , Viz Nagar, Mehsana, Gujarat, India .
authors.forename: Priya
authors.initials: P
authors.lastname: Sahni
authors.affiliation: Reader, Department of Oral & Maxillofacial Pathology, Vyas Dental College and Hospital , Pali road, near Kudi Haud, Jodhpur, India .
authors.forename: Abhishek
authors.initials: A
authors.lastname: Singhvi
authors.affiliation: Reader, Department of Conservative Dentistry & Endodontics, Vyas Dental College and Hospital , Pali road, near Kudi Haud, Jodhpur, India .
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Hans
authors.affiliation: Assistant Professor, Department of Conservative Dentistry & Endodontics, Vyas Dental College and Hospital , Pali road, near Kudi Haud, Jodhpur, India .
authors.forename: Amrit Singh
authors.initials: AS
authors.lastname: Ahluwalia
keywords: Antibiotic resistance; Caries pathogens; Gram stain; Kirby bauer method; Pseudomonas aeruginosa; Staphylococcusspecies; Streptococcus species