title: Combined effect of Cd and Pb spiked field soils on bioaccumulation, DNA damage, and peroxidase activities in Trifolium repens.
abstract: The present study was designed to investigate the combined effects of Cd and Pb on accumulation and genotoxic potential in white clover (Trifolium repens). For this purpose, T. repens was exposed to contaminated soils (2.5-20 mg kg(-1) cadmium (Cd), 250-2000 mg kg(-1) lead (Pb) and a mixture of these two heavy metals) for 3, 10 and 56 days. The resulting bioaccumulation of Cd and Pb, DNA damage (comet assay) and peroxidase activities (APOX and GPOX) were determined. The exposure time is a determinant factor in experiments designed to measure the influence of heavy metal contamination. The accumulation of Cd or Pb resulting from exposure to the two-metal mixture does not appear to depend significantly on whether the white clover is exposed to soil containing one heavy metal or both. However, when T. repens is exposed to a Cd/Pb mixture, the percentage of DNA damage is lower than when the plant is exposed to monometallic Cd. DNA damage is close to that observed in the case of monometallic Pb exposure. Peroxidase activity cannot be associated with DNA damage under these experimental conditions.
authors.affiliation: Laboratoire des Sciences Végétales et Fongiques (EA 4483), Faculté des Sciences Pharmaceutiques et Biologiques, Université Lille Droit et Santé, F-59006, Lille Cedex, France. caroline.lanier@univ-lille2.fr.|Faculté Ingénierie et Management de la Santé (EA 4483), Université Lille Droit et Santé, 42 rue Ambroise Paré, F-59120, Loos, France. caroline.lanier@univ-lille2.fr.
authors.forename: C
authors.initials: C
authors.lastname: Lanier
authors.affiliation: Laboratoire des Sciences Végétales et Fongiques (EA 4483), Faculté des Sciences Pharmaceutiques et Biologiques, Université Lille Droit et Santé, F-59006, Lille Cedex, France.|LGCgE (EA 4515), Université Lille Sciences et Technolohgies, Cité scientifique, bâtiment SN3-Rdc, F-59655, Villeneuve d'Ascq, France.
authors.forename: F
authors.initials: F
authors.lastname: Bernard
authors.affiliation: Laboratoire des Sciences Végétales et Fongiques (EA 4483), Faculté des Sciences Pharmaceutiques et Biologiques, Université Lille Droit et Santé, F-59006, Lille Cedex, France.
authors.forename: S
authors.initials: S
authors.lastname: Dumez
authors.affiliation: Laboratoire des Sciences Végétales et Fongiques (EA 4483), Faculté des Sciences Pharmaceutiques et Biologiques, Université Lille Droit et Santé, F-59006, Lille Cedex, France.
authors.forename: J
authors.initials: J
authors.lastname: Leclercq
authors.affiliation: LGCgE (EA 4515), Université Lille Sciences et Technolohgies, Cité scientifique, bâtiment SN3-Rdc, F-59655, Villeneuve d'Ascq, France.
authors.forename: S
authors.initials: S
authors.lastname: Lemière
authors.affiliation: LGCgE (EA 4515), Université Lille Sciences et Technolohgies, Cité scientifique, bâtiment SN3-Rdc, F-59655, Villeneuve d'Ascq, France.
authors.forename: F
authors.initials: F
authors.lastname: Vandenbulcke
authors.affiliation: Laboratoire de Toxicologie Génétique (EA 4483), Institut Pasteur de Lille, 1 rue du Pr Calmette BP245, F-59019, Lille Cedex, France.
authors.forename: F
authors.initials: F
authors.lastname: Nesslany
authors.affiliation: Laboratoire de Toxicologie Génétique (EA 4483), Institut Pasteur de Lille, 1 rue du Pr Calmette BP245, F-59019, Lille Cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Platel
authors.affiliation: Laboratoire des Sciences Végétales et Fongiques (EA 4483), Faculté des Sciences Pharmaceutiques et Biologiques, Université Lille Droit et Santé, F-59006, Lille Cedex, France.
authors.forename: I
authors.initials: I
authors.lastname: Devred
authors.affiliation: Laboratoire des Sciences Végétales et Fongiques (EA 4483), Faculté des Sciences Pharmaceutiques et Biologiques, Université Lille Droit et Santé, F-59006, Lille Cedex, France.
authors.forename: D
authors.initials: D
authors.lastname: Cuny
authors.affiliation: Laboratoire des Sciences Végétales et Fongiques (EA 4483), Faculté des Sciences Pharmaceutiques et Biologiques, Université Lille Droit et Santé, F-59006, Lille Cedex, France.|Faculté Ingénierie et Management de la Santé (EA 4483), Université Lille Droit et Santé, 42 rue Ambroise Paré, F-59120, Loos, France.
authors.forename: A
authors.initials: A
authors.lastname: Deram
mesh_terms: D002104:Cadmium / Q000032:analysis / Q000494:pharmacology*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004787:Environmental Pollution; D007854:Lead / Q000032:analysis / Q000494:pharmacology*; D010544:Peroxidases / Q000032:analysis / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D012989:Soil Pollutants / Q000032:analysis / Q000494:pharmacology*; D029921:Trifolium / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics
chemical_list: D010940:Plant Proteins; D012989:Soil Pollutants; D002104:Cadmium; D007854:Lead; D010544:Peroxidases
keywords: Comet assay; Heavy metals; Higher plants; Metal interactions; Oxidative stress; White clover