title: Electrocatalytic interconversion of NADH and NAD(+) by Escherichia coli flavohemoglobin.
abstract: E. coli flavohemoglobin, oriented at electrodes via amphiphilic polymyxin B, electrocatalytically interconverts NADH and NAD(+) at its heme potentials operating as an electron transfer relay between the electrode and the protein FAD, where NADH/NAD(+) is transformed. The results are crucial for the development of NAD(+)-dependent bioelectrodes for biosynthesis, biosensors and biofuel cells.
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark. elena.ferapontova@inano.au.dk.
authors.forename: S
authors.initials: S
authors.lastname: Shipovskov
authors.affiliation: Department of Biochemical Sciences and CNR Institute of Molecular Biology and Pathology, University "La Sapienza", 00185 Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Bonamore
authors.affiliation: Department of Biochemical Sciences and CNR Institute of Molecular Biology and Pathology, University "La Sapienza", 00185 Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Boffi
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark. elena.ferapontova@inano.au.dk.
authors.forename: E E
authors.initials: EE
authors.lastname: Ferapontova
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D004563:Electrochemistry; D004926:Escherichia coli / Q000378:metabolism*; D006420:Hemeproteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D009243:NAD / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D001426:Bacterial Proteins; D006420:Hemeproteins; C086193:flavohemoprotein, Bacteria; D009243:NAD