title: Live Edwardsiella tarda vaccine enhances innate immunity by metabolic modulation in zebrafish.
abstract: Control of bacterial infection resides in the core of human health and sustainable animal breeding. Vaccines as an economic and efficient immunoprophylaxis have been widely accepted, but mechanisms for vaccines do not fully understand. Information regarding to metabolome in response to vaccines is not available. Here we explore the metabolic features by using GC/MS based metabolic profile and trace metabolic mechanisms in zebrafish (Dario rerio) in response to live Edwardsiella tarda vaccine. Pathway enrichment analysis shows that live vaccine activates biosynthesis of unsaturated fatty acids and the TCA cycle and reduces aminoacyl-tRNA biosynthesis, suggesting a metabolic characteristic feature in response to the live vaccine. We further demonstrate that hydroxyl radical is limited during stimulation. Finally, we reveal oleate induces effective protection against E. tarda infection. These results have implications for immunity study that metabolic regulation contributes to immune protection. Our findings enable us to propose novel therapeutic strategies on metabolism against bacterial infections.
authors.affiliation: Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, PR China.
authors.forename: Chang
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Biological Sciences, The University of Texas, El Paso, TX 79968, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.affiliation: Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Song
authors.affiliation: Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, PR China.
authors.forename: Chang-wen
authors.initials: CW
authors.lastname: Wu
authors.affiliation: Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, PR China.
authors.forename: Man-jun
authors.initials: MJ
authors.lastname: Yang
authors.affiliation: Department of Biological Sciences, The University of Texas, El Paso, TX 79968, USA.
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, PR China. Electronic address: lihui32@sysu.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D020609:Edwardsiella tarda / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000662:veterinary*; D005393:Fish Diseases / Q000276:immunology*; D007113:Immunity, Innate; D055442:Metabolome; D014613:Vaccines, Attenuated / Q000276:immunology; D015027:Zebrafish
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated
keywords: Edwardsiella tarda; Fatty acids; Immunometabolism; Metabolomics; TCA cycle