title: Full-color structured illumination optical sectioning microscopy.
abstract: In merits of super-resolved resolution and fast speed of three-dimensional (3D) optical sectioning capability, structured illumination microscopy (SIM) has found variety of applications in biomedical imaging. So far, most SIM systems use monochrome CCD or CMOS cameras to acquire images and discard the natural color information of the specimens. Although multicolor integration scheme are employed, multiple excitation sources and detectors are required and the spectral information is limited to a few of wavelengths. Here, we report a new method for full-color SIM with a color digital camera. A data processing algorithm based on HSV (Hue, Saturation, and Value) color space is proposed, in which the recorded color raw images are processed in the Hue, Saturation, Value color channels, and then reconstructed to a 3D image with full color. We demonstrated some 3D optical sectioning results on samples such as mixed pollen grains, insects, micro-chips and the surface of coins. The presented technique is applicable to some circumstance where color information plays crucial roles, such as in materials science and surface morphology.
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Dan
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
authors.forename: Baoli
authors.initials: B
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Min
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
authors.forename: Xianghua
authors.initials: X
authors.lastname: Yu
mesh_terms: D000465:Algorithms; D000818:Animals; D001517:Coleoptera / Q000033:anatomy & histology; D003116:Color; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D008027:Light; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D008925:Mites / Q000033:anatomy & histology; D006109:Poaceae / Q000648:ultrastructure; D011058:Pollen / Q000648:ultrastructure; D012815:Signal Processing, Computer-Assisted; D013499:Surface Properties