title: A ddRAD Based Linkage Map of the Cultivated Strawberry, Fragaria xananassa.
abstract: The cultivated strawberry (Fragaria ×ananassa Duch.) is an allo-octoploid considered difficult to disentangle genetically due to its four relatively similar sub-genomic chromosome sets. This has been alleviated by the recent release of the strawberry IStraw90 whole genome genotyping array. However, array resolution relies on the genotypes used in the array construction and may be of limited general use. SNP detection based on reduced genomic sequencing approaches has the potential of providing better coverage in cases where the studied genotypes are only distantly related from the SNP array's construction foundation. Here we have used double digest restriction-associated DNA sequencing (ddRAD) to identify SNPs in a 145 seedling F1 hybrid population raised from the cross between the cultivars Sonata (♀) and Babette (♂). A linkage map containing 907 markers which spanned 1,581.5 cM across 31 linkage groups representing the 28 chromosomes of the species. Comparing the physical span of the SNP markers with the F. vesca genome sequence, the linkage groups resolved covered 79% of the estimated 830 Mb of the F. × ananassa genome. Here, we have developed the first linkage map for F. × ananassa using ddRAD and show that this technique and other related techniques are useful tools for linkage map development and downstream genetic studies in the octoploid strawberry.
authors.affiliation: Norwegian Institute for Bioeconomy Research, Ås, Norway.
authors.forename: Jahn
authors.initials: J
authors.lastname: Davik
authors.affiliation: Fondazione Edmund Mach, Centre for Research and Innovation, San Michele all'Adige, Italy.
authors.forename: Daniel James
authors.initials: DJ
authors.lastname: Sargent
authors.affiliation: Norwegian Institute for Bioeconomy Research, Ås, Norway.
authors.forename: May Bente
authors.initials: MB
authors.lastname: Brurberg
authors.affiliation: Center for Integrative Genetics, Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, Ås, Norway.
authors.forename: Sigbjørn
authors.initials: S
authors.lastname: Lien
authors.affiliation: Center for Integrative Genetics, Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, Ås, Norway.
authors.forename: Matthew
authors.initials: M
authors.lastname: Kent
authors.affiliation: Graminor Breeding Ltd., Ridabu, Norway; Department of Plant Sciences, Norwegian University of Life Sciences, Ås, Norway.
authors.forename: Muath
authors.initials: M
authors.lastname: Alsheikh
mesh_terms: D002874:Chromosome Mapping; D020090:Chromosome Segregation; D003433:Crosses, Genetic; D031985:Fragaria / Q000235:genetics / Q000254:growth & development; D008040:Genetic Linkage; D005819:Genetic Markers; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers