title: Tumor Infiltrating T Lymphocytes and Apoptosis in Colorectal Cancer.
abstract: Dysfunction of the immune system in colorectal cancer (CRC) can be due to a number of reasons including apoptosis of tumour infiltrating lymphocytes (TILs). The aims of this study was to investigate TILs in colorectal cancer and characterize apoptosis of TILs using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay for detecting DNA fragments. We used monoclonal antibodies (mAbs) to T lymphocytes to detect TILs and double immunohistochemistry to assess apoptosis. T lymphocytes were detected in the immune infiltrate in CRC. TUNEL staining disclosed a high level of cell death among TILs. Apoptosis of T lymphocytes showed significant correlation with Dukes' stage (P = 0.02), lymphatic metastasis (P = 0.03), vascular metastasis (P = 0.01), lymph node metastasis (P = 0.02) and age of patient (P = 0.01). In conclusion, CRC may elude immunological surveillance by inducing apoptosis of TILs.
authors.forename: Manal M S
authors.initials: MM
authors.lastname: Youssef
authors.forename: Emma C
authors.initials: EC
authors.lastname: Paish
authors.forename: J Clifford
authors.initials: JC
authors.lastname: Murray
authors.forename: Naglaa M
authors.initials: NM
authors.lastname: Farag
authors.forename: Khaled
authors.initials: K
authors.lastname: Saleh
authors.forename: I O
authors.initials: IO
authors.lastname: Ellis
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000401:mortality / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D053208:Kaplan-Meier Estimate; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology