title: Precise Identification of Genome-Wide Transcription Start Sites in Bacteria by 5'-Rapid Amplification of cDNA Ends (5'-RACE).
abstract: Transcription start sites are commonly used to locate promoter elements in bacterial genomes. TSS were previously studied one gene at a time, often through 5'-rapid amplification of cDNA ends (5'-RACE). This technique has now been adapted for high-throughput sequencing and can be used to precisely identify TSS in a genome-wide fashion for practically any bacterium, which greatly contributes to our understanding of gene regulatory networks in microorganisms.
authors.affiliation: Département de biologie, Faculté des sciences, Université de Sherbrooke, 2500 boulevard de l'Université, Sherbrooke, QC, Canada, J1K 2R1.
authors.forename: Dominick
authors.initials: D
authors.lastname: Matteau
authors.affiliation: Département de biologie, Faculté des sciences, Université de Sherbrooke, 2500 boulevard de l'Université, Sherbrooke, QC, Canada, J1K 2R1. sebastien.rodrigue@usherbrooke.ca.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Rodrigue
mesh_terms: D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D053263:Gene Regulatory Networks / Q000235:genetics; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D011401:Promoter Regions, Genetic; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary
keywords: 5′-RACE; Genome wide; Next-generation sequencing; Promoter; RNA polymerase holoenzyme; RNA-seq; Rapid amplification of cDNA ends; Transcription; Transcription start site; Transcriptomics