title: Repression of hTERT transcription by the introduction of chromosome 3 into human oral squamous cell carcinoma.
abstract: Telomerase is a ribonucleoprotein enzyme that maintains telomere length. Telomerase activity is primarily attributed to the expression of telomerase reverse transcriptase (TERT). It has been reported that introduction of an intact human chromosome 3 into the human oral squamous cell carcinoma cell line HSC3 suppresses the tumorigenicity of these cells. However, the mechanisms that regulate tumorigenicity have not been elucidated. To determine whether this reduction in tumorigenicity was accompanied by a reduction in telomerase activity, we investigated the transcriptional activation of TERT in HSC3 microcell hybrid clones with an introduced human chromosome 3 (HSC3#3). HSC#3 cells showed inhibition of hTERT transcription compared to that of the parental HSC3 cells. Furthermore, cell fusion experiments showed that hybrids of HSC3 cells and cells of the RCC23 renal carcinoma cell line, which also exhibits suppression of TERT transcription by the introduction of human chromosome 3, also displayed suppressed TERT transcription. These results suggested that human chromosome 3 may carry functionally distinct, additional TERT repressor genes.
authors.affiliation: Division of Oral and Maxillofacial Biopathological Surgery, Faculty of Medicine, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan; Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Tottori, 683-8503, Japan.
authors.forename: Sachiyo
authors.initials: S
authors.lastname: Nishio
authors.affiliation: Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Tottori, 683-8503, Japan.
authors.forename: Takahito
authors.initials: T
authors.lastname: Ohira
authors.affiliation: Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Tottori, 683-8503, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Sunamura
authors.affiliation: Chromosome Engineering Research Center, Tottori University, Yonago, Tottori, 683-8503, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Oshimura
authors.affiliation: Division of Oral and Maxillofacial Biopathological Surgery, Faculty of Medicine, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ryoke
authors.affiliation: Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Tottori, 683-8503, Japan; Chromosome Engineering Research Center, Tottori University, Yonago, Tottori, 683-8503, Japan. Electronic address: kugoh@med.tottori-u.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kugoh
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000235:genetics*; D045744:Cell Line, Tumor; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D005809:Genes, Regulator; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D009062:Mouth Neoplasms / Q000201:enzymology* / Q000235:genetics*; D019098:Telomerase / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D019098:Telomerase
keywords: Chromosome 3; Oral squamous cell carcinoma; Telomerase; Tumor suppressor gene; hTERT