title: Depiction of carbohydrate-active enzyme diversity in Caldicellulosiruptor sp. F32 at the genome level reveals insights into distinct polysaccharide degradation features.
abstract: Thermophilic bacterium Caldicellulosiruptor sp. F32 can utilize cellulose-, hemicellulose-containing biomass, including unpretreated wheat straw. We have conducted a bioinformatics analysis of the carbohydrate-active enzyme (CAZyme) in the genome of Caldicellulosiruptor sp. F32, which reveals a broad substrate range of the strain. Among 2285 predicted open reading frames (ORFs), 73 (3.2%) CAZyme encoding genes, including 44 glycoside hydrolases (GHs) distributing in 22 GH families, 6 carbohydrate esterases (CEs), 3 polysaccharide lyases (PLs), 21 glycosyl transferases (GTs), and 25 carbohydrate-binding modules (CBMs) were found. An in-depth bioinformatics analysis of CAZyme families that target cellulose, hemicellulose, chitin, pectin, starch, and β-1,3-1,4-glucan degradation were performed to highlight specialized polysaccharide degrading abilities of strain F32. A great number of orthologous multimodular CAZymes of Caldicellulosiruptor sp. F32 were found in other strains of genus Caldicellulosiruptor. While, a portion of the CAZymes of Caldicellulosiruptor sp. F32 showed sequence identity with proteins from strains of genus Clostridium. A thermostable β-glucosidase BlgA synergistically facilitated the enzymatic degradation of Avicel by endo-1,4-β-glucanase CelB, which indicated that the synchronous action of synergism between CAZymes enhanced the lignocellulose degradation by Caldicellulosiruptor sp. F32.
authors.affiliation: Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China. lifl@qibebt.ac.cn.
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Meng
authors.forename: Yu
authors.initials: Y
authors.lastname: Ying
authors.forename: Kun-Di
authors.initials: KD
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Lu
authors.forename: Fu-Li
authors.initials: FL
authors.lastname: Li
mesh_terms: D001419:Bacteria / Q000201:enzymology* / Q000235:genetics*; D002482:Cellulose / Q000378:metabolism; D002686:Chitin / Q000378:metabolism; D004795:Enzyme Stability; D016680:Genome, Bacterial; D006026:Glycoside Hydrolases / Q000378:metabolism*; D008031:Lignin; D016366:Open Reading Frames; D010368:Pectins / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D013213:Starch / Q000378:metabolism; D013696:Temperature
chemical_list: D011134:Polysaccharides; C036909:lignocellulose; D002686:Chitin; C007916:hemicellulose; D010368:Pectins; D002482:Cellulose; D013213:Starch; D008031:Lignin; D006026:Glycoside Hydrolases