title: Diterpenoids from the Endophytic Fungus Botryosphaeria sp. P483 of the Chinese Herbal Medicine Huperzia serrata.
abstract: Two new tetranorlabdane diterpenoids, named botryosphaerins G (1) and H (2), were isolated from the solid fermentation products of Botryosphaeria sp. P483 along with seven known tetranorlabdane diterpenes (3-9). Their structures were elucidated by extensive analysis, including 1D and 2D nuclear magnetic resonance (NMR) spectroscopy, and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS). Their absolute configuration was confirmed by single-crystal X-ray diffraction analyses using the anomalous scattering of Cu Kα radiation. All of the isolated compounds were tested for activity against phytopathogenic fungi and nematodes. Compounds 2 and 3 showed antifungal activity and compound 2 showed weak nematicidal activity.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. zouchengkm@126.com.|School of Pharmaceutical Sciences, Kunming Medical University, Kunming 650500, China. zouchengkm@126.com.
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. yyh8612@126.com.
authors.forename: Yin-He
authors.initials: YH
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. lixiaonian@mail.kib.ac.cn.
authors.forename: Xiao-Nian
authors.initials: XN
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Sciences, Kunming Medical University, Kunming 650500, China. zouchengkm@126.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. pjzhao@outlook.com.
authors.forename: Pei-Ji
authors.initials: PJ
authors.lastname: Zhao
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D000969:Antinematodal Agents / Q000737:chemistry / Q000494:pharmacology*; D018360:Crystallography, X-Ray; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D060026:Endophytes / Q000737:chemistry / Q000502:physiology; D032501:Huperzia / Q000382:microbiology; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008968:Molecular Conformation; D004718:Saccharomycetales / Q000737:chemistry* / Q000502:physiology; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000935:Antifungal Agents; D000969:Antinematodal Agents; D004224:Diterpenes
keywords: Botryosphaeria sp. P483; X-ray crystallography; antifungal activity; nematicidal activity; tetranorlabdane diterpenoid