title: Effects of methanolic extracts of edible plants on RAGE in high-glucose-induced human endothelial cells.
abstract: Advanced glycation end products' (AGEs) engagement of a cell-surface receptor for AGEs (RAGE) has been causally implicated in the pathogenesis of vascular complications in diabetic patients. Methanolic extracts from edible plants (MEEP) are naturally occurring phenolic compounds. The phenolic compounds have been reported to possess potent radical-scavenging properties. We investigated whether MEEP could inhibit high glucose-induced RAGE production through interference with reactive oxygen species generation in endothelial cells (ECs). ECs were incubated with 4.5 g/l of glucose in culture medium treated with 21 MEEP. Determination of RAGE production in the culture supernatants was performed by colorimetric ELISA. DNA damage was determined by using the 8-hydroxydeoxyguanosine ELISA kit. Because peroxynitrite radicals with stronger toxicity were produced by nitric oxide radical (NO), the NO scavenging activity of MEEP was assessed as nitrite generation. Peroxynitrite radical-dependent oxidation inhibition by MEEP was estimated by the Crow method. The results showed that four extracts reduced RAGE production. The extract from onion peel showed the highest RAGE production inhibition activity, followed by that of onion rhizome, cow pea and burdock. The results showed that RAGE production is correlated with the above-mentioned indicators. This study supports the utilization of four extracts for improved treatment of diabetic complications.
authors.affiliation: Nutrition Section, Ageing and Nutrition Research, Yms Laboratory, Gifu, Japan.
authors.forename: Mizue
authors.initials: M
authors.lastname: Okada
authors.affiliation: Laboratory on Ageing and Health Management, Graduate School of Nursing and Health, Aichi Prefectural University, Nagoya, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Okada
mesh_terms: D002478:Cells, Cultured; D004249:DNA Damage / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D016166:Free Radical Scavengers / Q000008:administration & dosage; D005947:Glucose / Q000008:administration & dosage*; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006801:Humans; D000432:Methanol / Q000737:chemistry; D010636:Phenols / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D010945:Plants, Edible / Q000737:chemistry*; D017382:Reactive Oxygen Species; D000067759:Receptor for Advanced Glycation End Products / Q000378:metabolism*
chemical_list: C000593871:AGER protein, human; D016166:Free Radical Scavengers; D017127:Glycation End Products, Advanced; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D000067759:Receptor for Advanced Glycation End Products; D005947:Glucose; D000432:Methanol
keywords: RAGE; edible plants; free radical scavenging activity; human umbilical vein endothelial cells; methanolic extracts