title: Circulating microRNA expression profile in B-cell acute lymphoblastic leukemia.
abstract: BACKGROUND
  Acute lymphoblastic leukemia (ALL) is a highly diverse disease characterized by cytogenetic and molecular abnormalities, including altered microRNA (miRNA) expression signatures.
  
  
  OBJECTIVE
  We perform and validate a plasma miRNA expression profiling to identify potential miRNA involved in leukemogenesis
  
  
  METHODS
  MiRNA expression profiling assay was realized in 39 B-ALL and 7 normal control plasma samples using TaqMan Low Density Array (TLDA) plates on Applied Biosystems 7900 HT Fast Real-Time PCR System. MiRNA validation was done for six miRNA differentially expressed by quantitative real-time PCR.
  
  
  RESULTS
  Seventy-seven circulating miRNA differentially expressed: hsa-miR-511, -222, and -34a were overexpressed, whereas hsa-miR-199a-3p, -223, -221, and -26a were underexpressed (p values < 0.005 for both sets). According to operating characteristic curve analysis, hsa-miR-511 was the most valuable biomarker for distinguishing B-ALL from normal controls, with an area under curve value of 1 and 100% for sensitivity, and specificity respectively.
  
  
  CONCLUSIONS
  Measuring circulating levels of specific miRNA implicated in regulation of cell differentiation and/or cell proliferation such as hsa-miRNA-511, offers high sensitivity and specificity in B-ALL detection and may be potentially useful for detection of disease progression, as indicator of therapeutic response, and in the assessment of biological and/or therapeutic targets for patients with B-ALL.
authors.affiliation: Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey NL, México.
authors.forename: Claudia Maribel
authors.initials: CM
authors.lastname: Luna-Aguirre
authors.affiliation: Laboratorio de Medicina Molecular, Unidad Académica de Medicina Humana y Ciencias de la Salud, Universidad Autónoma de Zacatecas, Zacatecas, México.
authors.forename: Margarita
authors.initials: M
authors.lastname: de la Luz Martinez-Fierro
authors.affiliation: Departamento de Biología Celular y Genética, Facultad de Ciencias Biológicas, Monterrey NL, México.
authors.forename: Fermín
authors.initials: F
authors.lastname: Mar-Aguilar
authors.affiliation: Laboratorio de Medicina Molecular, Unidad Académica de Medicina Humana y Ciencias de la Salud, Universidad Autónoma de Zacatecas, Zacatecas, México.
authors.forename: Idalia
authors.initials: I
authors.lastname: Garza-Veloz
authors.affiliation: Departamento de Ciencias Computacionales, ITESM, Campus Monterrey, Monterrey NL, México.
authors.forename: Víctor
authors.initials: V
authors.lastname: Treviño-Alvarado
authors.affiliation: Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey NL, México.|Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo León, Monterrey, México.
authors.forename: Augusto
authors.initials: A
authors.lastname: Rojas-Martinez
authors.affiliation: Servicio de Hematología, Hospital Universitario Universidad Autónoma de Nuevo León, Monterrey NL, México.
authors.forename: Jose Carlos
authors.initials: JC
authors.lastname: Jaime-Perez
authors.affiliation: Departamento de Biología Celular y Genética, Facultad de Ciencias Biológicas, Monterrey NL, México.
authors.forename: Guadalupe Ismael
authors.initials: GI
authors.lastname: Malagon-Santiago
authors.affiliation: Servicio de Hematología, Hospital Universitario Universidad Autónoma de Nuevo León, Monterrey NL, México.
authors.forename: Cesar Homero
authors.initials: CH
authors.lastname: Gutierrez-Aguirre
authors.affiliation: Servicio de Hematología, Hospital Universitario Universidad Autónoma de Nuevo León, Monterrey NL, México.
authors.forename: Oscar
authors.initials: O
authors.lastname: Gonzalez-Llano
authors.affiliation: Servicio de Hematología, Hospital Universitario Universidad Autónoma de Nuevo León, Monterrey NL, México.
authors.forename: Rosario
authors.initials: R
authors.lastname: Salazar-Riojas
authors.affiliation: Instituto Nacional de Medicina Genómica, México D.F. Periférico Sur, México.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Hidalgo-Miranda
authors.affiliation: Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey NL, México.
authors.forename: Herminia Guadalupe
authors.initials: HG
authors.lastname: Martinez-Rodriguez
authors.affiliation: Servicio de Hematología, Hospital Universitario Universidad Autónoma de Nuevo León, Monterrey NL, México.
authors.forename: David
authors.initials: D
authors.lastname: Gomez-Almaguer
authors.affiliation: Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey NL, México.|Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo León, Monterrey, México.
authors.forename: Rocio
authors.initials: R
authors.lastname: Ortiz-Lopez
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002648:Child; D002675:Child, Preschool; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007223:Infant; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood*; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: Biomarkers; hematological malignancies; qRT-PCR; signaling pathway