title: Integrative network analysis of rifampin-regulated miRNAs and their functions in human hepatocytes.
abstract: Rifampin is an important drug used in the treatment of tuberculosis, and it increases the drug metabolism in human hepatocytes. Previous studies have shown that rifampin can indirectly influence drug deposition through the regulation of molecular interactions of miRNA, PXR and other genes. The potential functions of miRNAs associated with rifampin- induced drug disposition are poorly understood. In this study, significantly differentially expressed miRNAs (SDEM) were extracted and used to predict the miRNA-regulated co-expression target genes (MCeTG). Additionally, a miRNA-regulated co-expressed protein interaction network (MCePIN) was constructed for SDEM by extending from the protein interaction network (PIN). The functioning of the miRNAs were analyzed using GO analysis and KEGG pathway enrichment analysis. A total of 20 miRNAs belonging to SDEM were identified, and 632 miRNA-regulated genes were predicted. The MCePIN was constructed by extending from PIN, and 10 miRNAs and 33 genes that are relevant to 7 functions, including response to wounding, wound healing, response to drug, defense response, inflammatory response, liver development and drug metabolism, were discerned. The results provided by this study offer valuable insights into the effect of rifampin on miRNAs, genes and protein levels.
authors.affiliation: College of Automation, Harbin Engineering University, 145 Nantong Street, Nangang District, Harbin, Heilongjiang, 150001, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Automation, Harbin Engineering University, 145 Nantong Street, Nangang District, Harbin, Heilongjiang, 150001, China.|Network Information Center, Qiqihar University, No. 42, Wenhua Street, Qiqihar, Heilongjiang, 161006, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Automation, Harbin Engineering University, 145 Nantong Street, Nangang District, Harbin, Heilongjiang, 150001, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Automation, Harbin Engineering University, 145 Nantong Street, Nangang District, Harbin, Heilongjiang, 150001, China.|Department of Medical and Molecular Genetics, Indiana University School of Medicine, 355 West 16th Street, Suite 4100, Indianapolis, IN 46202, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Liang
authors.affiliation: College of Automation, Harbin Engineering University, 145 Nantong Street, Nangang District, Harbin, Heilongjiang, 150001, China.
authors.forename: Weixing
authors.initials: W
authors.lastname: Feng
authors.affiliation: College of Automation, Harbin Engineering University, 145 Nantong Street, Nangang District, Harbin, Heilongjiang, 150001, China.
authors.forename: Xianglian
authors.initials: X
authors.lastname: Meng
authors.affiliation: College of Automation, Harbin Engineering University, 145 Nantong Street, Nangang District, Harbin, Heilongjiang, 150001, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Cong
authors.affiliation: College of Automation, Harbin Engineering University, 145 Nantong Street, Nangang District, Harbin, Heilongjiang, 150001, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000904:Antibiotics, Antitubercular / Q000008:administration & dosage; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D025941:Protein Interaction Mapping / Q000379:methods; D020543:Proteome / Q000378:metabolism*; D012293:Rifampin / Q000008:administration & dosage*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D018511:Systems Integration
chemical_list: D000904:Antibiotics, Antitubercular; D035683:MicroRNAs; D020543:Proteome; D012293:Rifampin
keywords: P450; PPI network; Rifampin; gene; hepatocyte; miRNA