title: The ABCG transporter PEC1/ABCG32 is required for the formation of the developing leaf cuticle in Arabidopsis.
abstract: The cuticle is an essential diffusion barrier on aerial surfaces of land plants whose structural component is the polyester cutin. The PERMEABLE CUTICLE1/ABCG32 (PEC1) transporter is involved in plant cuticle formation in Arabidopsis. The gpat6 pec1 and gpat4 gapt8 pec1 double and triple mutants are characterized. Their PEC1-specific contributions to aliphatic cutin composition and cuticle formation during plant development are revealed by gas chromatography/mass spectrometry and Fourier-transform infrared spectroscopy. The composition of cutin changes during rosette leaf expansion in Arabidopsis. C16:0 monomers are in higher abundance in expanding than in fully expanded leaves. The atypical cutin monomer C18:2 dicarboxylic acid is more prominent in fully expanded leaves. Findings point to differences in the regulation of several pathways of cutin precursor synthesis. PEC1 plays an essential role during expansion of the rosette leaf cuticle. The reduction of C16 monomers in the pec1 mutant during leaf expansion is unlikely to cause permeability of the leaf cuticle because the gpat6 mutant with even fewer C16:0 monomers forms a functional rosette leaf cuticle at all stages of development. PEC1/ABCG32 transport activity affects cutin composition and cuticle structure in a specific and non-redundant fashion.
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Biophore Building, CH-1015, Lausanne, Switzerland.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Fabre
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Biophore Building, CH-1015, Lausanne, Switzerland.
authors.forename: Imène
authors.initials: I
authors.lastname: Garroum
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Biophore Building, CH-1015, Lausanne, Switzerland.|Department of Chemistry, University of Wroclaw, 14 F. Joliot-Curie, 50-383, Wroclaw, Poland.
authors.forename: Sylwester
authors.initials: S
authors.lastname: Mazurek
authors.affiliation: Electron Microscopy Facility, University of Lausanne, Biophore Building, CH-1015, Lausanne, Switzerland.
authors.forename: Jean
authors.initials: J
authors.lastname: Daraspe
authors.affiliation: Electron Microscopy Facility, University of Lausanne, Biophore Building, CH-1015, Lausanne, Switzerland.
authors.forename: Antonio
authors.initials: A
authors.lastname: Mucciolo
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Biophore Building, CH-1015, Lausanne, Switzerland.
authors.forename: Martial
authors.initials: M
authors.lastname: Sankar
authors.affiliation: Electron Microscopy Facility, University of Lausanne, Biophore Building, CH-1015, Lausanne, Switzerland.
authors.forename: Bruno M
authors.initials: BM
authors.lastname: Humbel
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Biophore Building, CH-1015, Lausanne, Switzerland.
authors.forename: Christiane
authors.initials: C
authors.lastname: Nawrath
mesh_terms: D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D029681:Arabidopsis Proteins; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D008563:Membrane Lipids / Q000378:metabolism*; D009154:Mutation; D010539:Permeability; D019441:Plant Epidermis / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D030821:Plants, Genetically Modified
chemical_list: C577839:ABCG32 protein, Arabidopsis; D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; D008563:Membrane Lipids; C000521:cutin
keywords: At1g01610; At2g26910; At2g38110; At4g00400; cuticle; cutin; epidermis; organ fusion