title: [Separation and authentication of tilianin and quality standards of semen of Dracocephalum moldavia].
abstract: Tilianin was separated and authenticated from the seeds of Dracocephalum moldavia, a Uygur medicine, by chromatographic technique and spectroscopic method. The purity of tilianin is more than 98% determined by HPLC area normalization method. Thin layer chromatography (TLC) method was used to separate tilianin from D. moldavia by mixture of chloroform-methanol (5: 1) as a developing solvent on high performance silicagel precoated plate (SGF254) and using aluminium trichloride as a chromogenic agent for qualitative identification of D. moldavia. To establish a HPLC method for quantitative analysis of D. moldavia, tilianin was used as a Quantitative marker and separated on a C18 (4.6 mm x 250 mm, 5 μm) column with acetonitrile-01% formic acid (25: 75) as the mobile phase and detected at 330 nm. The calibration curve of tilianin displayed ideal linearity over the range of 0.617 2-123.44 μg x mL(-1) with a regression equation of Y = 33.773X - 0.824 8 (r = 1). The average recovery of tilianin was 101.0% with RSD of 3.7%. The RSD values of intra-day and inter-day precision were less than 2%. The content of tilianin in 4 batches of the authenticated semen of D. Moldavia was between 0.016 and 0.187 mg x g(-1). The qualitative and quantitative method established is suitable for the quality evaluation and assessment of semen of D. Moldavia.
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Cheng
authors.forename: Ting-yun
authors.initials: TY
authors.lastname: Ma
authors.lastname: Su Ley-man
authors.lastname: Ha-Lik
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Mu
authors.lastname: Tiann Fang
authors.forename: Gui-Xin
authors.initials: GX
authors.lastname: Chou
authors.forename: Zheng-tao
authors.initials: ZT
authors.lastname: Wang
authors.forename: Chang-hong
authors.initials: CH
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification* / Q000592:standards; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification* / Q000592:standards; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification* / Q000592:standards; D019686:Lamiaceae / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006027:Glycosides; C426884:tilianin