title: [In vivo imaging of blood flow using two-photon laser scanning fluorescent microscopy].
abstract: OBJECTIVE
  To observe the three-dimensional distribution of vessels, and to establish a new method for measurement of blood flow velocity in mice cerebral cortex using two-photon laser scanning microscopy and fluorescence probe labeling technique.
  
  
  METHODS
  The mouse was made cranial window surgery and injected Texas-Red through tail vein after anesthetized. The three-dimensional imaging of vessel was obtained through z-stack scanning, and blood flow velocity was quantified through line scanning.
  
  
  RESULTS
  We could detect vascular distribution for more than 500 µm depth using two-photon microscopy. The velocity of blood flow was (0.59 ± 0.12) mm/s in capillary.
  
  
  CONCLUSIONS
  The method for observing the brain blood flow by two-photon microscopy was established, which could achieve quantification of single vascular blood flow velocity and provide experimental evidence for basic research and medical applications.
authors.forename: Shuang-shuang
authors.initials: SS
authors.lastname: Liu
authors.forename: Ji-yun
authors.initials: JY
authors.lastname: Huang
authors.forename: Gui-feng
authors.initials: GF
authors.lastname: Xiao
authors.forename: Wei
authors.initials: W
authors.lastname: Yin
authors.forename: Zhao-Xiao-Nan
authors.initials: ZX
authors.lastname: Lin
authors.forename: Ying-mei
authors.initials: YM
authors.lastname: Lu
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D001921:Brain / Q000098:blood supply*; D002196:Capillaries; D002560:Cerebrovascular Circulation; D005456:Fluorescent Dyes; D006439:Hemodynamics; D051379:Mice; D008856:Microscopy, Fluorescence
chemical_list: D005456:Fluorescent Dyes