title: Intranasal vaccination with killed Leishmania amazonensis promastigotes antigen (LaAg) associated with CAF01 adjuvant induces partial protection in BALB/c mice challenged with Leishmania (infantum) chagasi.
abstract: The CAF01 adjuvant has previously been shown to be safe for human use and to be a potent adjuvant for several vaccine antigens. In the present work, we sought to optimize the Leishmania amazonensis antigens (LaAg) intranasal vaccine in an attempt to enhance the protective immune responses against Leishmania (infantum) chagasi by using the CAF01 association. LaAg/CAF01 vaccinated mice that were challenged 15 days after booster dose with L. (infantum) chagasi showed a significant reduction in their parasite burden in both the spleen and liver, which is associated with an increase in specific production of IFN-γ and nitrite, and a decrease in IL-4 production. In addition, LaAg/CAF01 intranasal delivery was able to increase lymphoproliferative immune responses after parasite antigen recall. These results suggest the feasibility of using the intranasal route for the delivery of crude antigens and of a human-compatible adjuvant against visceral leishmaniasis.
authors.affiliation: Núcleo de Doenças Infecciosas/Núcleo de Biotecnologia,Universidade Federal do Espírito Santo - UFES,Vitória,Brazil.
authors.forename: Janine Miranda
authors.initials: JM
authors.lastname: Leal
authors.affiliation: Núcleo de Doenças Infecciosas/Núcleo de Biotecnologia,Universidade Federal do Espírito Santo - UFES,Vitória,Brazil.
authors.forename: Marcelle
authors.initials: M
authors.lastname: Mosquini
authors.affiliation: Núcleo de Doenças Infecciosas/Núcleo de Biotecnologia,Universidade Federal do Espírito Santo - UFES,Vitória,Brazil.
authors.forename: Luciana Polaco
authors.initials: LP
authors.lastname: Covre
authors.affiliation: Núcleo de Núcleo de Biotecnologia,Universidade Federal do Espírito Santo - UFES,Vitória,Brazil.
authors.forename: Nataly Pescinalli
authors.initials: NP
authors.lastname: Stagmiller
authors.affiliation: Núcleo de Doenças Infecciosas/Núcleo de Biotecnologia,Universidade Federal do Espírito Santo - UFES,Vitória,Brazil.
authors.forename: Rodrigo Ribeiro
authors.initials: RR
authors.lastname: Rodrigues
authors.affiliation: Department of Infectious Disease and Immunology,Statens Serum Institut,Copenhagen,Denmark.
authors.forename: Dennis
authors.initials: D
authors.lastname: Christensen
authors.forename: Herbert Leonel
authors.initials: HL
authors.lastname: de Matos Guedes
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro,Rio de Janeiro,Brazil.
authors.forename: Herbert Leonel De Matos
authors.initials: HL
authors.lastname: Guedes
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro,Rio de Janeiro,Brazil.
authors.forename: Bartira
authors.initials: B
authors.lastname: Rossi-Bergmann
authors.affiliation: Núcleo de Doenças Infecciosas/Núcleo de Biotecnologia,Universidade Federal do Espírito Santo - UFES,Vitória,Brazil.
authors.forename: Daniel Cláudio De Oliviera
authors.initials: DC
authors.lastname: Gomes
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000281:Administration, Intranasal; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D000939:Epitopes; D005260:Female; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000097:blood; D018314:Leishmania infantum / Q000276:immunology*; D007894:Leishmania mexicana / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000517:prevention & control*; D008099:Liver / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D009573:Nitrites / Q000378:metabolism; D016052:Protozoan Vaccines / Q000008:administration & dosage*; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000469:parasitology; D015164:Vaccines, Inactivated / Q000008:administration & dosage
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016207:Cytokines; D000939:Epitopes; D007074:Immunoglobulin G; D009573:Nitrites; D016052:Protozoan Vaccines; D015164:Vaccines, Inactivated
keywords: CAF01 adjuvant; Leishmania infantum chagasi; intranasal vaccination; LaAg