title: Pattern of CsICE1 expression under cold or drought treatment and functional verification through analysis of transgenic Arabidopsis.
abstract: CsICE1 is thought to be involved in hardiness resistance of tea plants. Using seedling cuttings of biennial Wuniuzao in this study, the pattern of CsICE1 expression under cold temperature (4°, -5°C), drought [20% polyethylene glycol 6000 (PEG-6000)], and plant hormone [200 mg/L abscisic acid (ABA), 1 mg/L brassinolide (BR)] treatment was studied by real-time quantitative PCR. Additionally, stress resistance, such as the freezing resistance of CsICE1, was studied using Arabidopsis lines transformed with sense or anti-sense CsICE1 via Agrobacterium tumefaciens infection. Our results showed that CsICE1 mRNA could be induced under -5°C, PEG, ABA, or BR treatment, although the pattern of expression differed for all treatments. Compared to wild type (WT) and anti-sense ICE1 transgenic lines, sense lines displayed higher relative germination rates under salt and drought stress. After freezing treatment, the sense transgenic lines over-expressing CsICE1 showed a higher survival rate, increased levels of proline, and decreased levels of malonaldehyde. Conversely, compared with WT, anti-sense ICE1 transgenic lines had lower proline levels and higher malonaldehyde levels under freezing conditions. Our study indicates that CsICE1 is an important anti-freezing gene and that over-expression of CsICE1 can improve cold resistance and enhance salt and drought tolerance of transgenic lines.
authors.affiliation: Tea Research Institute, Qingdao Agricultural University, Qingdao, Shandong, China.
authors.forename: Z T
authors.initials: ZT
authors.lastname: Ding
authors.affiliation: Tea Research Institute, Qingdao Agricultural University, Qingdao, Shandong, China.
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.affiliation: Tea Research Institute, Qingdao Agricultural University, Qingdao, Shandong, China.
authors.forename: H
authors.initials: H
authors.lastname: Shi
authors.affiliation: Tea Research Institute, Qingdao Agricultural University, Qingdao, Shandong, China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: Tea Research Institute, Qingdao Agricultural University, Qingdao, Shandong, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D028241:Camellia sinensis / Q000235:genetics*; D003080:Cold Temperature; D003681:Dehydration; D055864:Droughts; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D024861:Genetic Enhancement; D018525:Germination; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation
chemical_list: D014157:Transcription Factors; D000040:Abscisic Acid