title: Shell matters: Magnetic targeting of SPIONs and in vitro effects on endothelial and monocytic cell function.
abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) are versatile and easily functionalized agents with high potential for diagnostic and therapeutic intravascular applications. In this study, we analyzed the responses of endothelial (ECs) and monocytic cells to three different types of SPIONs, in order to assess the influence of physico-chemical properties on the biological reactions to SPIONs. The following formulations were used: (1) Lauric acid-coated and BSA-stabilized SPION-1,(2) Lauric acid/BSA-coated SPION-2 and (3) dextran-coated SPION-3. SPION-1 were strongly internalized by ECs and reduced their viability in static conditions. Additionally, they had a dose-dependent inhibitory effect on monocytic cell chemotaxis to MCP-1, but did not affect monocytic cell recruitment by ECs. SPION-2 uptake was less pronounced, both in ECs and monocytic cells, and these particles were better tolerated by the vascular cells. Not being internalized by endothelial or monocytic cells, SPION-3 did not induce relevant effects on cell viability, motility or endothelial-monocytic cell interactions.Taken together, localized accumulation of circulating SPION under physiologic-like flow conditions and their cellular uptake depends on the physicochemical characteristics. Our findings suggest that SPION-2 are suitable for magnetic targeting of atherosclerotic plaques. Due to their excellent biocompatibility and low internalization, SPION-3 may represent a suitable imaging agent for intravascular applications.
authors.affiliation: Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, Erlangen, Germany.
authors.forename: Jasmin
authors.initials: J
authors.lastname: Matuszak
authors.affiliation: Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, Erlangen, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Dörfler
authors.affiliation: Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, Erlangen, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Zaloga
authors.affiliation: Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, Erlangen, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Unterweger
authors.affiliation: Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, Erlangen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Lyer
authors.affiliation: Laboratory of Molecular Cardiology, Department of Cardiology and Angiology, University Hospital Erlangen, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Dietel
authors.affiliation: Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, Erlangen, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Alexiou
authors.affiliation: Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, Erlangen, Germany.
authors.forename: Iwona
authors.initials: I
authors.lastname: Cicha
mesh_terms: D002470:Cell Survival; D002478:Cells, Cultured; D005290:Ferric Compounds; D052203:Ferrosoferric Oxide; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology*; D006801:Humans; D008280:Magnetics; D053768:Metal Nanoparticles; D009000:Monocytes / Q000166:cytology*
chemical_list: D005290:Ferric Compounds; C427127:superparamagnetic blood pool agent; C000499:ferric oxide; D052203:Ferrosoferric Oxide
keywords: Atherosclerosis; SPION uptake; endothelial migration; endothelial-monocytic cell interactions; live-cell analysis; magnetic nanoparticles; monocytic cell chemotaxis