title: [Research on quality standards of Euonymus fortunei].
abstract: To establish quality standards of Euonymus fortunei, and supply scientific evidence for the quality control of Euonymus fortunei. Empirical and microscopic identification methods were adopted to observe morphological and histological characters. The contents of water, total ash, acid-insoluble ash and alcohol-soluble extractive were analysed according to the methods of Chinese Pharmaco- poeia (2010). Dulcitol and reference herbs were used to identify materia medica of Euonymus fortunei by TLC method. The total flavonol glycosides contents were analysed by HPLC method, using quercetin and kaempferol as reference substances. Quercetin and kaempferol were separated on a C18 column (4.6 mm x 250 mm, 5 μm) with methanol-0.1% formic acid(51:49) as the mobile phase and detected at 366 nm. The flavonoid aglycones content was then multiplied by a conversion coefficient, and the result was the total flavonol glycosides content. The macroscopical identification, microscopic features and TLC methods were proper. The average contents of water, total ash, acid-insoluble ash, alcohol-soluble extractive and total flavonol glycosides were 8.76%, 6.48%, 0.31%, 17.48% and 0.211% , respectively. The quality standards established on the basis of the research results were suitable for the quality evaluation of Euonymus fortunei.
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Yan
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Xue-feng
authors.initials: XF
authors.lastname: Feng
authors.forename: Qi-wei
authors.initials: QW
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Shen
authors.forename: Xiao-qian
authors.initials: XQ
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhu
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Zhang
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: Wang
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D029756:Euonymus / Q000033:anatomy & histology / Q000737:chemistry*; D013058:Mass Spectrometry; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal