title: Development of SPR biosensor for simultaneous detection of multiplex respiratory viruses.
abstract: A surface plasmon resonance (SPR)-based biosensor was developed for specific detection of nine common respiratory virus, including influenza A and influenza B, H1N1, respiratory syncytial virus (RSV), parainfluenza virus 1-3 (PIV1, 2, 3), adenovirus, and severe acute respiratory syndrome coronavirus (SARS). The SPR biosensor was developed by immobilizing nine respiratory virus-specific oligonucleotides in an SPR chip. To increase the biosensor sensitivity, biotin was used to label the PCR primer and further amplify the signal by introducing streptavidin after hybridization. Throat swab specimens representing nine common respiratory viruses were tested by the innovative SPR-based biosensor to evaluate the sensitivity, specificity and reproducibility of this method. Results suggest that this biosensor has the potential to simultaneously identify common respiratory viruses.
authors.affiliation: Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou, 510632, P.R. China.|Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518010, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: Feng Gang Hospital, Dongguan, 523695, P.R. China.
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China.
authors.forename: Jian'an
authors.initials: J
authors.lastname: He
authors.affiliation: Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.affiliation: Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China.
authors.forename: Chunzhong
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China.
authors.forename: Yunqing
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China.
authors.forename: Changlin
authors.initials: C
authors.lastname: Wu
authors.affiliation: Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou, 510632, P.R. China.
authors.forename: Junjian
authors.initials: J
authors.lastname: Xiang
authors.affiliation: Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Gu
authors.affiliation: Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Long
authors.affiliation: Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, P.R. China.
authors.forename: Hekui
authors.initials: H
authors.lastname: Lan
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000175:diagnosis / Q000821:virology; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology; D029524:Betainfluenzavirus / Q000235:genetics / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000821:virology; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000302:isolation & purification*; D018108:Respirovirus / Q000235:genetics / Q000302:isolation & purification*; D010253:Respirovirus Infections / Q000175:diagnosis / Q000821:virology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis / Q000821:virology; D020349:Surface Plasmon Resonance / Q000379:methods*
keywords: SPR; gene chip; high throughput; label-free; respiratory virus