title: Superoxide Dismutase Activity, Hydrogen Peroxide Steady-State Concentration, and Bactericidal and Phagocytic Activities Against Moraxella bovis, in Neutrophils Isolated from Copper-Deficient Bovines.
abstract: Copper (Cu) deficiency increases occurrence of certain infectious diseases in animals, including infectious keratoconjunctivitis in bovines, a bacterial ocular inflammation caused by Moraxella bovis. Neutrophil leukocytes constitute the first phagocytic cells to arrive at infection sites for bacterial neutralization. The objective of this work was to evaluate whether the functionality of neutrophils against M. bovis is impaired in experimentally induced Cu deficiency in bovines using high molybdenum and sulfur levels in the diet. The Cu tissue values and the periocular achromotrichia observed in +Mo animals showed that the clinic phase of Cu deficiency was reached in this group. Instead, +Cu animals have not evidenced clinical signs or biochemical parameters of hypocuprosis. On the basis of our observations, we concluded that Cu deficiency has no effect on phagocytic and bactericidal activities of neutrophils against M. bovis. However, superoxide dismutase activity and peroxide hydrogen generation were significantly different between groups. Therefore, additional research to explain these results is merited to fully characterize the consequences of Cu status on the risk for infections under field conditions.
authors.affiliation: Cátedra de Patología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires (UBA), Chorroarín 280, C1427CWO, Buenos Aires, Argentina. gabrielapostma@yahoo.com.ar.
authors.forename: Postma Gabriela
authors.initials: PG
authors.lastname: Cintia
authors.affiliation: Cátedra de Patología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires (UBA), Chorroarín 280, C1427CWO, Buenos Aires, Argentina.
authors.forename: Minatel
authors.initials: M
authors.lastname: Leonardo
authors.affiliation: Cátedra de Patología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires (UBA), Chorroarín 280, C1427CWO, Buenos Aires, Argentina.
authors.forename: Olivares Roberto Walter
authors.initials: OR
authors.lastname: Israel
authors.affiliation: Cátedra de Patología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires (UBA), Chorroarín 280, C1427CWO, Buenos Aires, Argentina.
authors.forename: Schapira
authors.initials: S
authors.lastname: Andrea
authors.affiliation: Cátedra de Fisicoquímica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Valdez Laura
authors.initials: VL
authors.lastname: Beatriz
authors.affiliation: Cátedra de Patología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires (UBA), Chorroarín 280, C1427CWO, Buenos Aires, Argentina.
authors.forename: Dallorso Maria
authors.initials: DM
authors.lastname: Elena
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000172:deficiency* / Q000378:metabolism*; D004789:Enzyme Activation; D006861:Hydrogen Peroxide / Q000032:analysis / Q000494:pharmacology*; D016955:Moraxella bovis / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects* / Q000382:microbiology; D010587:Phagocytosis / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D003300:Copper; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase
keywords: Calves; Copper; Innate immunity; Phagocytic cells; Respiratory burst; Trace elements