title: Helicobacter pylori cagA Promoter Region Sequences Influence CagA Expression and Interleukin 8 Secretion.
abstract: Heterogeneity at the Helicobacter pylori cagA gene promoter region has been linked to variation in CagA expression and gastric histopathology. Here, we characterized the cagA promoter and expression in 46 H. pylori strains from Portugal. Our results confirm the relationship between cagA promoter region variation and protein expression originally observed in strains from Colombia. We observed that individuals with intestinal metaplasia were all infected with H. pylori strains containing a specific cagA motif. Additionally, we provided novel functional evidence that strain-specific sequences in the cagA promoter region and CagA expression levels influence interleukin 8 secretion by the host gastric epithelial cells.
authors.affiliation: i3S - Instituto de Investigação e Inovação em Saúde Ipatimup - Institute of Molecular Pathology and Immunology.
authors.forename: Rui M
authors.initials: RM
authors.lastname: Ferreira
authors.affiliation: i3S - Instituto de Investigação e Inovação em Saúde Ipatimup - Institute of Molecular Pathology and Immunology.
authors.forename: Ines
authors.initials: I
authors.lastname: Pinto-Ribeiro
authors.affiliation: Ipatimup - Institute of Molecular Pathology and Immunology Centro Hospitalar Vila Nova de Gaia/Espinho, Portugal.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Wen
authors.affiliation: Institute of Biomedical Sciences Abel Salazar CIDES/CINTESIS Department of Gastroenterology, Centro Hospitalar do Porto.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Marcos-Pinto
authors.affiliation: CIDES/CINTESIS Department of Gastroenterology, IPO.
authors.forename: Mário
authors.initials: M
authors.lastname: Dinis-Ribeiro
authors.affiliation: i3S - Instituto de Investigação e Inovação em Saúde Ipatimup - Institute of Molecular Pathology and Immunology Faculty of Medicine, University of Porto Department of Pathology, Centro Hospitalar São João, Porto.
authors.forename: Fátima
authors.initials: F
authors.lastname: Carneiro
authors.affiliation: i3S - Instituto de Investigação e Inovação em Saúde Ipatimup - Institute of Molecular Pathology and Immunology Faculty of Medicine, University of Porto.
authors.forename: Ceu
authors.initials: C
authors.lastname: Figueiredo
mesh_terms: D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology*; D016022:Case-Control Studies; D004847:Epithelial Cells / Q000276:immunology / Q000382:microbiology; D005260:Female; D015870:Gene Expression; D014644:Genetic Variation; D016481:Helicobacter Infections / Q000276:immunology / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011174:Portugal; D011401:Promoter Regions, Genetic; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016209:Interleukin-8; C080866:cagA protein, Helicobacter pylori
keywords: CagA expression; Helicobacter pylori; IL-8; cagA promoter region