title: Nutraceutical intervention reverses the negative effects of blood from aged rats on stem cells.
abstract: Aging is associated with a decline in function in many of the stem cell niches of the body. An emerging body of literature suggests that one of the reasons for this decline in function is due to cell non-autonomous influences on the niche from the body. For example, studies using the technique of parabiosis have demonstrated a negative influence of blood from aged mice on muscle satellite cells and neurogenesis in young mice. We examined if we could reverse this effect of aged serum on stem cell proliferation by treating aged rats with NT-020, a dietary supplement containing blueberry, green tea, vitamin D3, and carnosine that has been shown to increase neurogenesis in aged rats. Young and aged rats were administered either control NIH-31 diet or one supplemented with NT-020 for 28 days, and serum was collected upon euthanasia. The serum was used in cultures of both rat hippocampal neural progenitor cells (NPCs) and rat bone marrow-derived mesenchymal stem cells (MSCs). Serum from aged rats significantly reduced cell proliferation as measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 5-bromo-2'-deoxyuridine (BrdU) assays in both NPCs and MSCs. Serum from aged rats treated with NT-020 was not different from serum from young rats. Therefore, NT-020 rescued the effect of serum from aged rats to reduce stem cell proliferation.
authors.affiliation: Research Service, James A Haley Veterans Affairs Hospital|, Tampa, FL, 33620, USA. pbickfor@health.usf.edu.|Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, MDC-78, USF Morsani College of Medicine, 12901 Bruce B Downs Blvd, Tampa, FL, 33620, USA. pbickfor@health.usf.edu.
authors.forename: Paula C
authors.initials: PC
authors.lastname: Bickford
authors.affiliation: Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, MDC-78, USF Morsani College of Medicine, 12901 Bruce B Downs Blvd, Tampa, FL, 33620, USA.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kaneko
authors.affiliation: Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, MDC-78, USF Morsani College of Medicine, 12901 Bruce B Downs Blvd, Tampa, FL, 33620, USA.|Department of Molecular Pharmacology and Physiology, USF Morsani College of Medicine, Tampa, FL, 33620, USA.
authors.forename: Bethany
authors.initials: B
authors.lastname: Grimmig
authors.affiliation: School of Aging Studies, USF, Tampa, FL, 33620, USA.
authors.forename: Colleen
authors.initials: C
authors.lastname: Pappas
authors.affiliation: School of Aging Studies, USF, Tampa, FL, 33620, USA.
authors.forename: Brent
authors.initials: B
authors.lastname: Small
authors.affiliation: Natura Therapeutics, Inc., Tampa, FL, USA.
authors.forename: Cyndy D
authors.initials: CD
authors.lastname: Sanberg
authors.affiliation: Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, MDC-78, USF Morsani College of Medicine, 12901 Bruce B Downs Blvd, Tampa, FL, 33620, USA.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Sanberg
authors.affiliation: Department of Psychiatry, USF Morsani College of Medicine, Tampa, FL, 33620, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Tan
authors.affiliation: Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, MDC-78, USF Morsani College of Medicine, 12901 Bruce B Downs Blvd, Tampa, FL, 33620, USA.
authors.forename: R
authors.initials: R
authors.lastname: Douglas Shytle
mesh_terms: D000375:Aging / Q000097:blood* / Q000187:drug effects*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D019587:Dietary Supplements; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects; D055495:Neurogenesis / Q000187:drug effects*; D051381:Rats; D011916:Rats, Inbred F344
keywords: Aging; NT-020; Proliferation; Stem cells