title: Role of IL-12 in overcoming the low responsiveness of NK cells to missing self after traumatic brain injury.
abstract: Blood samples from 32 patients with severe Traumatic brain injury (TBI) were studied and compared with 11 cardiac surgery patients, and 29 healthy controls. A dramatic decreased expression of HLA class I molecules on monocytes was associated with increased KIR+ NK cell frequency in TBI patients. Overall, the phenotype of TBI NK cells marked by KIR and CD57 expression and lower level of NKp46 and DNAM-1 reflected a differentiated state. The NK-cell response to missing self was marked by lower degranulation and lower IFN-γ production after stimulation with HLA class I deficient cell line. In contrast, the NK-cell ADCC was not altered. IL-12 was able to restore both IFN-γ production and the cytotoxicity capacities of NK cells. This study provides the first extensive description of the phenotype and functions of NK cells in TBI patients. Further evaluation of IL-12 treatment to overcome immunosuppression-induced nosocomial infections is warranted.
authors.affiliation: Intensive Care Unit, Anesthesia and Critical Care Department, Hôtel Dieu - HME, University Hospital of Nantes, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Roquilly
authors.affiliation: Intensive Care Unit, Anesthesia and Critical Care Department, Hôtel Dieu - HME, University Hospital of Nantes, France; Université de Nantes, Faculté de Médecine, Thérapeutiques Cliniques et Expérimentales des Infections, EA 3826 Nantes, France; Etablissement Français du Sang, Nantes, France; Equipe d'Accueil 4271, ImmunoVirologie et Polymorphisme Génétique, Université de Nantes, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: David
authors.affiliation: Intensive Care Unit, Anesthesia and Critical Care Department, Hôtel Dieu - HME, University Hospital of Nantes, France; Université de Nantes, Faculté de Médecine, Thérapeutiques Cliniques et Expérimentales des Infections, EA 3826 Nantes, France.
authors.forename: Raphael
authors.initials: R
authors.lastname: Cinotti
authors.affiliation: Intensive Care Unit, Anesthesia and Critical Care Department, Hôtel Dieu - HME, University Hospital of Nantes, France; Université de Nantes, Faculté de Médecine, Thérapeutiques Cliniques et Expérimentales des Infections, EA 3826 Nantes, France.
authors.forename: Mickaël
authors.initials: M
authors.lastname: Vourc'h
authors.affiliation: Intensive Care Unit, Anesthesia and Critical Care Department, Laennec, University Hospital of Nantes,Nantes, France.
authors.forename: Helene
authors.initials: H
authors.lastname: Morin
authors.affiliation: Intensive Care Unit, Anesthesia and Critical Care Department, Laennec, University Hospital of Nantes,Nantes, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Rozec
authors.affiliation: Etablissement Français du Sang, Nantes, France; Equipe d'Accueil 4271, ImmunoVirologie et Polymorphisme Génétique, Université de Nantes, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Retière
authors.affiliation: Intensive Care Unit, Anesthesia and Critical Care Department, Hôtel Dieu - HME, University Hospital of Nantes, France; Université de Nantes, Faculté de Médecine, Thérapeutiques Cliniques et Expérimentales des Infections, EA 3826 Nantes, France. Electronic address: karim.asehnoune@chu-nantes.fr.
authors.forename: Karim
authors.initials: K
authors.lastname: Asehnoune
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000070642:Brain Injuries, Traumatic / Q000276:immunology*; D015550:Cell Degranulation / Q000276:immunology; D005260:Female; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D018664:Interleukin-12; D007371:Interferon-gamma
keywords: HLA class I; IL-12; Immune suppression; KIR; Monocytes; NK cells; Traumatic brain injury