title: Benfotiamine upregulates antioxidative system in activated BV-2 microglia cells.
abstract: Chronic microglial activation and resulting sustained neuroinflammatory reaction are generally associated with neurodegeneration. Activated microglia acquires proinflammatory cellular profile that generates oxidative burst. Their persistent activation exacerbates inflammation, which damages healthy neurons via cytotoxic mediators, such as superoxide radical anion and nitric oxide. In our recent study, we have shown that benfotiamine (S-benzoylthiamine O-monophosphate) possesses anti-inflammatory effects. Here, the effects of benfotiamine on the pro-oxidative component of activity of LPS-stimulated BV-2 cells were investigated. The activation of microglia was accompanied by upregulation of intracellular antioxidative defense, which was further promoted in the presence of benfotiamine. Namely, activated microglia exposed to non-cytotoxic doses of benfotiamine showed increased levels and activities of hydrogen peroxide- and superoxide-removing enzymes-catalase and glutathione system, and superoxide dismutase. In addition, benfotiamine showed the capacity to directly scavenge superoxide radical anion. As a consequence, benfotiamine suppressed the activation of microglia and provoked a decrease in NO and (·)O(-) 2 production and lipid peroxidation. In conclusion, benfotiamine might silence pro-oxidative activity of microglia to alleviate/prevent oxidative damage of neighboring CNS cells.
authors.affiliation: Institute for Biological Research "Siniša Stanković," University of Belgrade Belgrade, Serbia.
authors.forename: Iva
authors.initials: I
authors.lastname: Bozic
authors.affiliation: Institute for Biological Research "Siniša Stanković," University of Belgrade Belgrade, Serbia.
authors.forename: Danijela
authors.initials: D
authors.lastname: Savic
authors.affiliation: Institute for Medical Research, Military Medical Academy Belgrade, Serbia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Stevanovic
authors.affiliation: Institute for Biological Research "Siniša Stanković," University of Belgrade Belgrade, Serbia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Pekovic
authors.affiliation: Faculty of Biology, Institute for Physiology and Biochemistry, University of Belgrade Belgrade, Serbia.
authors.forename: Nadezda
authors.initials: N
authors.lastname: Nedeljkovic
authors.affiliation: Institute for Biological Research "Siniša Stanković," University of Belgrade Belgrade, Serbia.
authors.forename: Irena
authors.initials: I
authors.lastname: Lavrnja
keywords: LPS; benfotiamine; catalase; glutathione; microglia; oxidative stress