title: Both acute delivery of and storage with magnesium sulfate promote cold-stored platelet aggregation and coagulation function.
abstract: BACKGROUND
  The platelet storage lesion causes loss of function and viability over time. A new paradigm for platelet storage is desired to enable safer, more effective transfusions while reducing waste. We hypothesized that repletion of Mg, which is chelated by citrate anticoagulant, could reduce platelet storage lesion severity when given in conjunction with storage at a refrigerated temperature.
  
  
  METHODS
  Apheresis platelet units were collected from healthy donors and stored at 22°C or 4°C. On Days 0, 2, 4, and 8, samples were collected for analyses of receptor-mediated aggregation, coagulation, adhesion to collagen under flow, and viability. In the first series, samples were given an acute dose of MgSO4 before testing; in the second series, storage bags were supplemented with 0-, 3-, or 6-mM MgSO4.
  
  
  RESULTS
  Acutely delivered MgSO4 induced a more rapid coagulation time in apheresis platelets, further enhanced by storage at 4°C. Platelet adhesion to a collagen surface while exposed to arterial shear rates (920 s) was enhanced by MgSO4 supplementation-acute MgSO4 had a large effect on adhesion of fresh platelets, which diminished more rapidly in 22°C samples, while storage with MgSO4 showed significant benefits even out to Day 4 at both temperatures. Although 4°C storage improves the longevity of platelet aggregation responses to agonists, MgSO4 supplementation did not change those responses.
  
  
  CONCLUSIONS
  Acute MgSO4 reduces clot time likely through the transient increase of free Ca. Limited differences between platelet function in acute delivery of and storage with MgSO4 diminish the possibility that Mg-induced metabolic inhibition of platelets synergizes with 4°C storage. Regardless, magnesium supplementation to platelets is an exciting possibility in transfusion because the adhesion response of 22°C-stored platelets on Day 4 is significantly enhanced when stored with 6-mM MgSO4.
authors.affiliation: From the Coagulation and Blood Research Group (M.A.M., A.C.R., M.V.V., A.P.C.), US Army Institute of Surgical Research; and Joint Inflammation Modulation of Trauma Program (J.E.C.), Tri-Service Research Laboratory.
authors.forename: M Adam
authors.initials: MA
authors.lastname: Meledeo
authors.forename: James E
authors.initials: JE
authors.lastname: Campbell
authors.forename: Armando C
authors.initials: AC
authors.lastname: Rodriguez
authors.forename: Melanie V
authors.initials: MV
authors.lastname: Valenciana
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Cap
mesh_terms: D001777:Blood Coagulation / Q000187:drug effects*; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D006801:Humans; D008278:Magnesium Sulfate / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010979:Platelet Function Tests
chemical_list: D008278:Magnesium Sulfate