title: Proteomic pilot study of tuberculosis pleural effusion.
abstract: To discuss the differences in protein expression among tuberculosis pleural effusion (TBPE), malignant pleural effusion (MPE) and transudative pleural effusion (TSPE). We recruited 50 patients with pleural effusion, including 20 TBPEs, 17 MPEs and 13 TSPEs. Using the two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS), we acquired the peptide mass finger printings (PMFs) of featured proteins. Then, we identified characteristic proteins by searching in the protein databank of the National Center of Biotechnology Information (NCBI) and assessed their diagnostic significance. We found five characteristic proteins: C1-inhibitor (C1-INH), transthyretin (TTR), human complement fragment 3b (C3b), human ceruloplasmin (CP), and Z34c protein fragment Fc (Z34c-Fc). C1-INH shows a high expression in TBPE and a low expression in MPE while TTR and C3 show low expression in TBPE and high expressions in MPE. Z34c-Fc and CP have a higher expression in TBPE than in TSPE. No common characteristic protein was found between MPE and TSPE. Statistic analysis consisted of paired t-tests and the difference between them is significance (P<0.05). C1-inhibitor (C1-INH), transthyretin (TTR), human Complement fragment 3b (C3b), human Ceruloplasmin (CP) and Z34c protein fragment Fc (Z34c-Fc) may provide an additional perspective in the differential diagnosis of PE.
authors.affiliation: The Second Department of Respiratory Medicine, The First Affiliated Hospital of Kunming Medical University, 295 #, Xichang Road, Xishan District, Kunming City, Yunnan Province, China.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The Second Department of Respiratory Medicine, The First Affiliated Hospital of Kunming Medical University, 295 #, Xichang Road, Xishan District, Kunming City, Yunnan Province, China.
authors.forename: Li-Zhou
authors.initials: LZ
authors.lastname: Fang
authors.affiliation: The Second Department of Respiratory Medicine, The First Affiliated Hospital of Kunming Medical University, 295 #, Xichang Road, Xishan District, Kunming City, Yunnan Province, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.affiliation: The Second Department of Respiratory Medicine, The First Affiliated Hospital of Kunming Medical University, 295 #, Xichang Road, Xishan District, Kunming City, Yunnan Province, China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The Second Department of Respiratory Medicine, The First Affiliated Hospital of Kunming Medical University, 295 #, Xichang Road, Xishan District, Kunming City, Yunnan Province, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Fu
authors.affiliation: The Second Department of Respiratory Medicine, The First Affiliated Hospital of Kunming Medical University, 295 #, Xichang Road, Xishan District, Kunming City, Yunnan Province, China.
authors.forename: Luming
authors.initials: L
authors.lastname: Dai
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D010865:Pilot Projects; D010996:Pleural Effusion / Q000150:complications* / Q000473:pathology; D016066:Pleural Effusion, Malignant / Q000150:complications* / Q000473:pathology; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014376:Tuberculosis / Q000150:complications* / Q000473:pathology
chemical_list: D011506:Proteins
keywords: 2-DE; MALDI-TOF-MS; Tuberculosis pleural effusion; proteomic