title: Variations in chemical fingerprints and major flavonoid contents from the leaves of thirty-one accessions of Hibiscus sabdariffa L.
abstract: The leaves of Hibiscus sabdariffa L. have been used as traditional folk medicines for treating high blood pressure and fever. There are many accessions of H. sabdariffa L. throughout the world. To assess the chemical variations of 31 different accessions of H. sabdariffa L., fingerprinting analysis and quantitation of major flavonoids were performed by high-performance liquid chromatography (HPLC). The HPLC method was validated for linearity, sensitivity, precision, repeatability and accuracy. A quadrupole-time-of-flight mass spectrometry (Q-TOF-MS) was applied for the characterization of major compounds. A total of 9 compounds were identified, including 6 flavonoids and 3 phenolic acids. In the fingerprint analysis, similarity analysis (SA) and principal component analysis (PCA) were used to differentiate the 31 accessions of H. sabdariffa L. Based on the results of PCA and SA, the samples No. 15 and 19 appeared much different from the main group. The total content of five flavonoids varied greatly among different accessions, ranging from 3.35 to 23.30 mg/g. Rutin was found to be the dominant compound and the content of rutin could contribute to chemical variations among different accessions. This study was helpful to understand the chemical variations between different accessions of H. sabdariffa L., which could be used for quality control. © 2015 The Authors Biomedical Chromatography Published by John Wiley & Sons Ltd.
authors.affiliation: SFA Key Laboratory of Bamboo and Rattan Science and Technology, International Centre for Bamboo and Rattan, No. 8 Futong Dongdajie, Wangjing, Chaoyang District, Beijing, 100102, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: SFA Key Laboratory of Bamboo and Rattan Science and Technology, International Centre for Bamboo and Rattan, No. 8 Futong Dongdajie, Wangjing, Chaoyang District, Beijing, 100102, China.
authors.forename: Xianshuang
authors.initials: X
authors.lastname: Cao
authors.affiliation: Southern University Agricultural Research and Extension Center, Baton Rouge, LA, 70813, USA.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Ferchaud
authors.affiliation: Southern University Agricultural Research and Extension Center, Baton Rouge, LA, 70813, USA.|Urban Forestry Program, College of Science and Agriculture, Southern University, Baton Rouge, LA, 70813, USA.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Qi
authors.affiliation: SFA Key Laboratory of Bamboo and Rattan Science and Technology, International Centre for Bamboo and Rattan, No. 8 Futong Dongdajie, Wangjing, Chaoyang District, Beijing, 100102, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.affiliation: SFA Key Laboratory of Bamboo and Rattan Science and Technology, International Centre for Bamboo and Rattan, No. 8 Futong Dongdajie, Wangjing, Chaoyang District, Beijing, 100102, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Tang
authors.affiliation: SFA Key Laboratory of Bamboo and Rattan Science and Technology, International Centre for Bamboo and Rattan, No. 8 Futong Dongdajie, Wangjing, Chaoyang District, Beijing, 100102, China.
authors.forename: Yongde
authors.initials: Y
authors.lastname: Yue
authors.affiliation: Southern University Agricultural Research and Extension Center, Baton Rouge, LA, 70813, USA.
authors.forename: Kit L
authors.initials: KL
authors.lastname: Chin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000032:analysis*; D031584:Hibiscus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D025341:Principal Component Analysis; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D005419:Flavonoids
keywords: Hibiscus sabdariffa L; LC-Q-TOF-MS; fingerprints; flavonoids; principal components analysis (PCA)