title: Dose Response of MARV/Angola Infection in Cynomolgus Macaques following IM or Aerosol Exposure.
abstract: Marburg virus infection in humans causes a hemorrhagic disease with a high case fatality rate. Countermeasure development requires the use of well-characterized animal models that mimic human disease. To further characterize the cynomolgus macaque model of MARV/Angola, two independent dose response studies were performed using the intramuscular or aerosol routes of exposure. All animals succumbed at the lowest target dose; therefore, a dose effect could not be determined. For intramuscular-exposed animals, 100 PFU was the first target dose that was not significantly different than higher target doses in terms of time to disposition, clinical pathology, and histopathology. Although a significant difference was not observed between aerosol-exposed animals in the 10 PFU and 100 PFU target dose groups, 100 PFU was determined to be the lowest target dose that could be consistently obtained and accurately titrated in aerosol studies.
authors.affiliation: Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, 21702, United States of America.
authors.forename: Sara C
authors.initials: SC
authors.lastname: Johnston
authors.affiliation: Nonclinical Development Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, 21702, United States of America.
authors.forename: Kenny L
authors.initials: KL
authors.lastname: Lin
authors.affiliation: Pathology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, 21702, United States of America.
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Twenhafel
authors.affiliation: Pathology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, 21702, United States of America.
authors.forename: Jo Lynne W
authors.initials: JL
authors.lastname: Raymond
authors.affiliation: Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, 21702, United States of America.
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Shamblin
authors.affiliation: Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, 21702, United States of America.
authors.forename: Suzanne E
authors.initials: SE
authors.lastname: Wollen
authors.affiliation: Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, 21702, United States of America.
authors.forename: Carly B
authors.initials: CB
authors.lastname: Wlazlowski
authors.affiliation: Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, 21702, United States of America.
authors.forename: Eric R
authors.initials: ER
authors.lastname: Wilkinson
authors.affiliation: Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, 21702, United States of America.
authors.forename: Miriam A
authors.initials: MA
authors.lastname: Botto
authors.affiliation: Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, 21702, United States of America.
authors.forename: Arthur J
authors.initials: AJ
authors.lastname: Goff
mesh_terms: D000336:Aerosols / Q000008:administration & dosage*; D000818:Animals; D007273:Injections, Intramuscular; D053208:Kaplan-Meier Estimate; D008252:Macaca fascicularis; D008379:Marburg Virus Disease / Q000097:blood / Q000821:virology*; D029024:Marburgvirus / Q000502:physiology*; D012367:RNA, Viral / Q000097:blood; D013696:Temperature
chemical_list: D000336:Aerosols; D012367:RNA, Viral