title: A paper-based detection method of cancer cells using the photo-thermal effect of nanocomposite.
abstract: A novel paper-based dot immune-graphene-gold filtration assay (DIGGFA) for the detection of breast cancer cells was developed based on the photo-thermal effect of graphene oxide (GO)-Au nanocomposite. Anti-EpCAM antibody which specific to the MCF-7 cell surface antigen, was immobilized on the nitrocellulose paper. The GO-Au-anti-EpCAM composite would interact with the MCF-7 cells captured on the nitrocellulose paper. After the test zone was irradiated by a laser, GO-Au nanocomposite could generate heat, temperature contrast was recorded and positive correlated with the cell number. Standard curve was prepared according to the temperature contrast and the cell number. Under optimal conditions, this method could detect a minimum of 600 MCF-7 cells with a near infrared laser and an infrared temperature gun within 15 min. This simple and rapid method could be applied to the clinical diagnosis in hospitals.
authors.affiliation: College of Life Science, Shandong Normal University, Jinan 250014, PR China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Life Science, Shandong Normal University, Jinan 250014, PR China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Life Science, Shandong Normal University, Jinan 250014, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Shandong Product Quality Inspection Research Institute, Jinan 31000, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Bing
authors.affiliation: Shandong Product Quality Inspection Research Institute, Jinan 31000, PR China.
authors.forename: Jingjun
authors.initials: J
authors.lastname: Hua
authors.affiliation: College of Life Science, Shandong Normal University, Jinan 250014, PR China. Electronic address: shwzhhy@163.com.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D005260:Female; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells; D053761:Nanocomposites / Q000737:chemistry*; D010209:Paper; D010777:Photochemistry / Q000379:methods*; D013816:Thermodynamics
chemical_list: D006046:Gold; D006108:Graphite
keywords: Cancer cell; Graphene nanocomposite; Paper-based analytical device; Photo-thermal effect