title: Rescheduling Behavioral Subunits of a Fixed Action Pattern by Genetic Manipulation of Peptidergic Signaling.
abstract: The ecdysis behavioral sequence in insects is a classic fixed action pattern (FAP) initiated by hormonal signaling. Ecdysis triggering hormones (ETHs) release the FAP through direct actions on the CNS. Here we present evidence implicating two groups of central ETH receptor (ETHR) neurons in scheduling the first two steps of the FAP: kinin (aka drosokinin, leucokinin) neurons regulate pre-ecdysis behavior and CAMB neurons (CCAP, AstCC, MIP, and Bursicon) initiate the switch to ecdysis behavior. Ablation of kinin neurons or altering levels of ETH receptor (ETHR) expression in these neurons modifies timing and intensity of pre-ecdysis behavior. Cell ablation or ETHR knockdown in CAMB neurons delays the switch to ecdysis, whereas overexpression of ETHR or expression of pertussis toxin in these neurons accelerates timing of the switch. Calcium dynamics in kinin neurons are temporally aligned with pre-ecdysis behavior, whereas activity of CAMB neurons coincides with the switch from pre-ecdysis to ecdysis behavior. Activation of CCAP or CAMB neurons through temperature-sensitive TRPM8 gating is sufficient to trigger ecdysis behavior. Our findings demonstrate that kinin and CAMB neurons are direct targets of ETH and play critical roles in scheduling successive behavioral steps in the ecdysis FAP. Moreover, temporal organization of the FAP is likely a function of ETH receptor density in target neurons.
authors.affiliation: Department of Entomology, University of California, Riverside, Riverside, California, United States of America.
authors.forename: Do-Hyoung
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, South Korea.
authors.forename: Mi-Ran
authors.initials: MR
authors.lastname: Han
authors.affiliation: Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee, United States of America.
authors.forename: Gyunghee
authors.initials: G
authors.lastname: Lee
authors.affiliation: Department of Cell Biology & Neuroscience, University of California, Riverside, Riverside, California, United States of America; Graduate Program in Neuroscience, University of California, Riverside, Riverside, California, United States of America.
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Lee
authors.affiliation: Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, South Korea.
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Entomology, University of California, Riverside, Riverside, California, United States of America; Department of Cell Biology & Neuroscience, University of California, Riverside, Riverside, California, United States of America; Graduate Program in Neuroscience, University of California, Riverside, Riverside, California, United States of America.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Adams
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D007301:Insect Hormones / Q000378:metabolism; D007705:Kinins / Q000378:metabolism; D018978:Molting; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D010455:Peptides / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D007301:Insect Hormones; D007705:Kinins; D010455:Peptides; D002118:Calcium