title: Growth and motility of human skin fibroblasts on multilayer strong polyelectrolyte films.
abstract: Polyelectrolyte multilayers (PEMs) have found application in modifying material surfaces to make them adhesive or non-adhesive for animal cells. However, PEMs made of strong polyelectrolytes are not fully recognized in the literature. This study focuses on the interplay between the properties of PEM assembled from strong polyelectrolytes and cell adhesion and motility. Strong polycations (with quaternary ammonium groups) and a polyanion (with sulfonate groups) were obtained by modification of poly(allylamine hydrochloride) (PAH). Two types of multilayer films were assembled from these PAH derivatives and used to investigate the behavior of human skin fibroblasts (HSFs). The effect of surface charge, hydrophobicity, and film thickness on adhesion of HSFs in a serum-containing medium was studied with immunofluorescence microscopy. The results showed that adhesion of HSFs was strongly depended on the chemical functions of the terminal layer, whereas the wettability was not important. The surface of PEM can be strongly cytophobic (the quaternary ammonium terminal groups) or strongly cytophilic (the sulfonate terminal groups). Finally, the motile activity of HSFs seeded on glass coated with a varying number of polymer layers was investigated. It was demonstrated using an in vitro model that coating the substrate with only two polymer layers can considerably increase the average speed of HSFs movement and stimulate cell migration into the wound.
authors.affiliation: Academic Centre for Materials and Nanotechnology, AGH University of Science and Technology, al. A. Mickiewicza 30, 30-059 Krakow, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wytrwal
authors.affiliation: Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland; Department of Pharmaceutical Biochermistry, Jagiellonian University Medical College, Medyczna 9, 30-688 Krakow, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Koczurkiewicz
authors.affiliation: Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Zrubek
authors.affiliation: Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30 Mickiewicza Av., 30-059 Kraków, Poland.
authors.forename: Wiktor
authors.initials: W
authors.lastname: Niemiec
authors.affiliation: Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Michalik
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
authors.forename: Bartłomiej
authors.initials: B
authors.lastname: Kozik
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
authors.forename: Edward
authors.initials: E
authors.lastname: Szneler
authors.affiliation: Academic Centre for Materials and Nanotechnology, AGH University of Science and Technology, al. A. Mickiewicza 30, 30-059 Krakow, Poland; Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, 30 Mickiewicza Av., 30-059 Kraków, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bernasik
authors.affiliation: Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Madeja
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Nowakowska
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland. Electronic address: kepczyns@chemia.uj.edu.pl.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Kepczynski
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004573:Electrolytes / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D012867:Skin / Q000166:cytology* / Q000187:drug effects; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D004573:Electrolytes; D011108:Polymers
keywords: Atomic force microscopy (AFM); Cell motility; Contact angle; Human skin fibroblasts; Layer-by-Layer (LbL); Poly(allylamine hydrochloride); Polyelectrolyte multilayer (PEM); Strong polyelectrolyte