title: Sensitive detection of Porcine circovirus-2 by droplet digital polymerase chain reaction.
abstract: Sensitive detection of Porcine circovirus-2 (PCV-2) is very important for surveillance of postweaning multisystemic wasting syndrome. Droplet digital polymerase chain reaction (ddPCR) is novel PCR method that can achieve high precision. Our study aimed to develop a sensitive assay utilizing ddPCR to detect PCV-2. Specificity of the assay was confirmed by the failure of amplification of DNA of other relevant viruses. The detection limit for ddPCR was 25 copies/μL, a 4-fold greater sensitivity than TaqMan real-time PCR. Both methods showed a high degree of linearity (R(2) = ~1), although TaqMan real-time PCR showed less sensitivity than ddPCR for clinical detection. Our findings indicate that ddPCR might represent a promising platform for detecting PCV-2 viral loads.
authors.affiliation: College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu).
authors.forename: Shan
authors.initials: S
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu).
authors.forename: Hua
authors.initials: H
authors.lastname: Lin
authors.affiliation: College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu).
authors.forename: Shijie
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu).
authors.forename: Miao
authors.initials: M
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu) yanqigui@126.com.
authors.forename: Qigui
authors.initials: Q
authors.lastname: Yan
authors.affiliation: College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu).
authors.forename: Caifang
authors.initials: C
authors.lastname: Wen
authors.affiliation: College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu).
authors.forename: Zhongxiang
authors.initials: Z
authors.lastname: Hao
authors.affiliation: College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu).
authors.forename: Yubao
authors.initials: Y
authors.lastname: Yan
authors.affiliation: College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu).
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu).
authors.forename: Juan
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu).
authors.forename: Zhenrong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu).
authors.forename: Lixin
authors.initials: L
authors.lastname: Xi
mesh_terms: D000818:Animals; D002681:China; D018173:Circoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D057230:Limit of Detection; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology; D019562:Viral Load / Q000662:veterinary
chemical_list: D004279:DNA, Viral
keywords: Detection; Porcine circovirus-2; TaqMan real-time PCR; droplet digital PCR