title: Biomimetic hybrid porous scaffolds immobilized with platelet derived growth factor-BB promote cellularization and vascularization in tissue engineering.
abstract: Development of hybrid scaffolds with synergistic combination of growth factor is a promising approach to promote early in vivo wound repair and tissue regeneration. Here, we show the rapid wound healing in Wistar albino rats using biomimetic collagen-poly(dialdehyde) guar gum based hybrid porous scaffolds covalently immobilized with platelet derived growth factor-BB. The immobilized platelet derived growth factor in the hybrid scaffolds not only enhance the total protein, collagen, hexosamine, and uronic acid contents in the granulation tissue but also provide stronger tissues. The wound closure analysis reveal that the complete epithelialization period is 15.4 ± 0.9 days for collagen-poly(dialdehyde) guar gum-platelet derived growth factor hybrid scaffolds, whereas it is significantly higher for control, collagen, collagen- poly(dialdehyde) guar gum and povidine-iodine treated groups. Further, the histological evaluation shows that the immobilized platelet derived growth factor in the hybrid scaffolds induced a more robust cellular and vascular response in the implanted site. Hence, we demonstrate that the collagen-poly(dialdehyde) guar gum hybrid scaffolds loaded with platelet derived growth factor stimulates chemotactic effects in the implanted site to promote rapid tissue regeneration and wound repair without the assistance of antibacterial agents.
authors.affiliation: Advanced Materials Laboratory, Centre for Leather Apparel & Accessories Development, Central Leather Research Institute (Council of Scientific and Industrial Research), Adyar, Chennai, 600020, India.
authors.forename: Ragothaman
authors.initials: R
authors.lastname: Murali
authors.affiliation: Department of Biochemistry, Central Leather Research Institute (Council of Scientific and Industrial Research), Adyar, Chennai, 600020, India.
authors.forename: Thangavel
authors.initials: T
authors.lastname: Ponrasu
authors.affiliation: Advanced Materials Laboratory, Centre for Leather Apparel & Accessories Development, Central Leather Research Institute (Council of Scientific and Industrial Research), Adyar, Chennai, 600020, India.
authors.forename: Kalirajan
authors.initials: K
authors.lastname: Cheirmadurai
authors.affiliation: Advanced Materials Laboratory, Centre for Leather Apparel & Accessories Development, Central Leather Research Institute (Council of Scientific and Industrial Research), Adyar, Chennai, 600020, India.
authors.forename: Palanisamy
authors.initials: P
authors.lastname: Thanikaivelan
mesh_terms: D000818:Animals; D000077214:Becaplermin; D040761:Biomimetic Materials / Q000737:chemistry*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D016062:Porosity; D020574:Proto-Oncogene Proteins c-sis / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D055767:Immobilized Proteins; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin
keywords: collagen; platelet-derived growth factor; poly(dialdehyde) guar gum; tissue regeneration; wound healing