title: Molecular cloning and expression analysis of neuregulin 1 (Nrg1) in the hypothalamus of Huoyan goose during different stages of the egg-laying cycle.
abstract: Neuregulin 1 (Nrg1) is one of the most active members of the epidermal growth factor (EGF)-like family, which bind to the ErbB tyrosine kinase receptor and play many roles in modulation of synaptic activity, synaptogenesis, GABAergic neurotransmission, neurotransmitter receptor expression and the hormonal control of neuroendocrine reproductive development. In this study, we cloned and characterized the cDNA of goose Nrg1 originating from hypothalamus tissues of Huoyan goose using RACE method, investigated the mRNA expression profiles during different stages of the egg-laying cycle by real-time PCR. Multiple alignments and phylogenetic analyses of the deduced amino acid sequence were conducted using bioinformatics tools. We also determined the profiles of blood serum progesterone, estradiol, FSH and LH content during different egg-laying stages using radioimmunoassay. The cDNA of Nrg1 is consisted of 2061bp open reading frame encoding 686 amino acids. The deduced amino acid sequence of goose Nrg1 contains one EGF domain from amino acid residues 224 to 265 and shows a closer genetic relationship to the avian species than to other mammal species. The expression level of Nrg1 mRNA increased from the pre-laying period to the peak-laying period, reached its peak in the peak-laying period, and then decreased in the ceased period. The concentrations of FSH and estradiol in blood serum have the similar changing trend. These results might suggest a potential correlation between Nrg1/ErbB signaling network with the reproductive neuroendocrine of Huoyan goose.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: caozhongzan@163.com.
authors.forename: Zhongzan
authors.initials: Z
authors.lastname: Cao
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: 2689558571@qq.com.
authors.forename: Dawei
authors.initials: D
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: meiliu2014@163.com.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: minggao2014@163.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Gao
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: 779594756@qq.com.
authors.forename: Zimo
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: 731994204@qq.com.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Xing
authors.affiliation: Liaoning Province Livestock and Poultry Genetic Resources Conservation and Utilization Center, Liaoyang 111000, China. Electronic address: hyzhang2014@163.com.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Guizhou Minzu University, Guiyang 550025, China. Electronic address: yinyunhou@163.com.
authors.forename: Yunhou
authors.initials: Y
authors.lastname: Yin
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address: xhluan@163.com.
authors.forename: Xinhong
authors.initials: X
authors.lastname: Luan
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D019295:Computational Biology / Q000379:methods; D004958:Estradiol / Q000097:blood; D005640:Follicle Stimulating Hormone / Q000097:blood; D005777:Geese / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000378:metabolism; D007986:Luteinizing Hormone / Q000097:blood; D020890:Neuregulin-1 / Q000235:genetics* / Q000378:metabolism; D010058:Oviposition; D010802:Phylogeny; D011374:Progesterone / Q000097:blood
chemical_list: D020890:Neuregulin-1; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
keywords: Huoyan goose; Hypothalamus; Nrg1; Race; Real-time PCR