title: [Etablishment of cartilage degeneration model by IL-1 beta in vitro].
abstract: OBJECTIVE
  To establish a reliable model for drug screening and therapy by culturing rat femoral head and inducing cartilage degeneration quickly in vitro.
  
  
  METHODS
  The femoral heads from the same SD rats of two-month old were divided into control group and experimental group respectively. They were cultured with DMEM medium plus 10% fetal bovine serum or DMEM medium plus 10% fetal bovine serum plus 50 ng/ml IL-1β for three days. Femoral heads were fixed in 4% paraformaldehyde, decalcified, dehydrated, embedded in paraffin and cut into slices. Specimens were stained with Toluidine blue and Safranine O-Fast Green FCF. The protein expression levels of type II collagen, MMP13, Sox9 and ADAMTS5 were analyzed by immunofluorescence.
  
  
  RESULTS
  Both the Toluidine blue and Safranine O staining were pale in the margin of femoral heads which were stimulated with IL-1β for three days compared to that in control group. The Fast Green FCF staining was positive at the edge of the femoral head in experimental group, which indicated that cartilage became degenerated. The expression levels of both type H collagen and Sox9 were decreased significantly while the expression levels of MMP13 and ADAMTS5 were increased in experimental group.
  
  
  CONCLUSIONS
  The model of cartilage degeneration is established by culturing and inducing the degeneration of the femoral heads quickly in vitro.
authors.forename: Dao-fang
authors.initials: DF
authors.lastname: Ding
authors.forename: Jian
authors.initials: J
authors.lastname: Pang
authors.forename: Yi
authors.initials: Y
authors.lastname: Song
authors.forename: Guo-qing
authors.initials: GQ
authors.lastname: Du
authors.forename: Yue-long
authors.initials: YL
authors.lastname: Cao
authors.forename: Hong-sheng
authors.initials: HS
authors.lastname: Zhan
authors.forename: Yu-xin
authors.initials: YX
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002357:Cartilage Diseases / Q000235:genetics / Q000378:metabolism*; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005270:Femur Head / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D055758:SOX9 Transcription Factor / Q000235:genetics / Q000378:metabolism
chemical_list: D024043:Collagen Type II; D053583:Interleukin-1beta; D055758:SOX9 Transcription Factor; D053509:Matrix Metalloproteinase 13