title: Magnetic Resonance Detection of CD34+ Cells from Umbilical Cord Blood Using a 19F Label.
abstract: Impaired homing and delayed recovery upon hematopoietic stem cell transplantation (HSCT) with hematopoietic stem cells (HSC) derived from umbilical cord blood (UCB) is a major problem. Tracking transplanted cells in vivo will be helpful to detect impaired homing at an early stage and allows early interventions to improve engraftment and outcome after transplantation. In this study, we show sufficient intracellular labeling of UCB-derived CD34+ cells, with 19F-containing PLGA nanoparticles which were detectable with both flow cytometry and magnetic resonance spectroscopy (MRS). In addition, labeled CD34+ cells maintain their capacity to proliferate and differentiate, which is pivotal for successful engraftment after transplantation in vivo. These results set the stage for in vivo tracking experiments, through which the homing efficiency of transplanted cells can be studied.
authors.affiliation: Department of Hematology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Lucia E
authors.initials: LE
authors.lastname: Duinhouwer
authors.affiliation: Department of Radiology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Bernard J M
authors.initials: BJ
authors.lastname: van Rossum
authors.affiliation: Department of Radiology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Sandra T
authors.initials: ST
authors.lastname: van Tiel
authors.affiliation: Department of Radiology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Ramon M
authors.initials: RM
authors.lastname: van der Werf
authors.affiliation: Department of Radiology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Gabriela N
authors.initials: GN
authors.lastname: Doeswijk
authors.affiliation: Department of Radiology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Joost C
authors.initials: JC
authors.lastname: Haeck
authors.affiliation: Department of Hematology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Elwin W J C
authors.initials: EW
authors.lastname: Rombouts
authors.affiliation: Department of Hematology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Mariëtte N D
authors.initials: MN
authors.lastname: Ter Borg
authors.affiliation: Department of Radiology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Gyula
authors.initials: G
authors.lastname: Kotek
authors.affiliation: Department of Hematology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Eric
authors.initials: E
authors.lastname: Braakman
authors.affiliation: Department of Hematology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Jan J
authors.initials: JJ
authors.lastname: Cornelissen
authors.affiliation: Department of Radiology, Erasmus University Medical Centre, Rotterdam, The Netherlands; Department of Nuclear Medicine, Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Monique R
authors.initials: MR
authors.lastname: Bernsen
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism*; D058948:Cell Tracking / Q000379:methods*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D005462:Fluorine Radioisotopes; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D018952:Antigens, CD34; D005462:Fluorine Radioisotopes; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid