title: Clinicomycological Study of Dermatophytosis in South India.
abstract: BACKGROUND
  Dermatophytic infections are commonly encountered a problem and constitute more than 50% of cases in dermatology outpatient departments. Diagnosis of these infections requires the proper use of laboratory methods.
  
  
  OBJECTIVE
  This study was conducted to know the etiology of dermatophytosis in patients attending Tertiary Care Level Hospital in South India and to compare the efficacy of Sabouraud's dextrose agar (SDA) with actidione and dermatophyte test medium (DTM) in isolating and identifying dermatophytes.
  
  
  METHODS
  A total of 110 samples which included 101 skin samples and 9 hair samples from clinically suspected dermatophytosis were collected. Direct microscopy by KOH and culture on SDA with actidione and DTM were done.
  
  
  RESULTS
  Of 110 samples collected, 58.18% were KOH positive for fungal filaments and 56.36% were culture positive for dermatophytes. More number of cases were observed between age groups of 21-40 years. Males were more affected compared to females. Tinea corporis was the common clinical presentation observed (40%). Trichophyton rubrum (58.06%) was the predominant isolate recovered in all clinical presentations but Trichophyton violaceum was the most common isolate in tinea capitis. All culture positives were grown on both SDA with actidione and DTM. Appearance of growth was earlier on DTM that is, within 10 days compared to SDA with actidione where growth started appearing only after 10 days. This is statistically significant P < 0.0001 (χ(2) = 71.6). Species level identification on primary isolation was possible when grown on SDA with actidione and it was not possible with the growth on DTM on primary isolation.
  
  
  CONCLUSIONS
  DTM is a good screening medium in laboratory diagnosis of dermatophytosis when compared to SDA with actidione. But DTM is inferior to SDA with actidione in identification of dermatophyte species.
authors.affiliation: Department of Microbiology, Kamineni Institute of Medical Sciences, Nalgonda, Telangana, India.
authors.forename: Lakshmi Vasantha
authors.initials: LV
authors.lastname: Poluri
authors.affiliation: Department of Microbiology, Andhra Medical College, Visakhapatnam, Andhra Pradesh, India.
authors.forename: Jyothi P
authors.initials: JP
authors.lastname: Indugula
authors.affiliation: Department of Microbiology, Kamineni Institute of Medical Sciences, Nalgonda, Telangana, India.
authors.forename: Sai L
authors.initials: SL
authors.lastname: Kondapaneni
keywords: Dermatophte test medium; Sabouraud's dextrose agar with actidione; dermatophytosis