title: Gene-expression Profiling in Non-small Cell Lung Cancer with Invasion of Mediastinal Lymph Nodes for Prognosis Evaluation.
abstract: OBJECTIVE
  The aim of the study was to determine the pathways and expression profile of the genes that might predict response to neoadjuvant chemotherapy in patients with stage IIIA non-small cell lung cancer (NSCLC).
  
  
  METHODS
  We evaluated, by microarray, the gene-expression profile of tumoral mediastinal lymph node samples surgically removed from 27 patients with stage IIIA NSCLC before neoadjuvant chemotherapy treatment. Depending on the response to the induction treatment, the patients were divided in two groups: group A: patients whose disease evolved, stabilized or who had minor response to chemotherapy, and group B: patients whose disease stabilized or had major response to chemotherapy.
  
  
  RESULTS
  The microarray experiments identified 1,127 genes with a modified expression in the tumoral tissue compared to normal tissue with p≤0.05 and 44 genes with p≤0.01. The identified up-regulated genes between tumoral versus normal tissue included collagen, type I, alpha 1 (COL1A1), inhibin beta A (INHBA) and thioredoxin interacting protein (TXNIP). Pathways identified with a false-discovery rate of <0.005 included: cytokine pathways, focal adhesion or extracellular matrix receptor interaction.
  
  
  CONCLUSIONS
  Our approach identified important characteristics of NSCLC and pointed-out molecular differences between sub-groups of patients based on their response to therapy.
authors.affiliation: Center of Digestive Diseases and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania madalinalidia@yahoo.com.
authors.forename: Madalina
authors.initials: M
authors.lastname: Grigoroiu
authors.affiliation: Intelligent Systems and Bioinformatics Laboratory (ISBL), Department of Computer Science - Wayne State University, Detroit, MI, U.S.A.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Tagett
authors.affiliation: Center of Digestive Diseases and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania.
authors.forename: Sorin
authors.initials: S
authors.lastname: Draghici
authors.affiliation: Center of Digestive Diseases and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania.
authors.forename: Simona
authors.initials: S
authors.lastname: Dima
authors.affiliation: Center of Digestive Diseases and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania.
authors.forename: Anca
authors.initials: A
authors.lastname: Nastase
authors.affiliation: Center of Digestive Diseases and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania.
authors.forename: Raluca
authors.initials: R
authors.lastname: Florea
authors.affiliation: Center of Digestive Diseases and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania.
authors.forename: Andrei
authors.initials: A
authors.lastname: Sorop
authors.affiliation: Center of Digestive Diseases and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania.
authors.forename: Veronica
authors.initials: V
authors.lastname: Ilie
authors.affiliation: Center of Digestive Diseases and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania.
authors.forename: Nicolae
authors.initials: N
authors.lastname: Bacalbasa
authors.affiliation: Center of Digestive Diseases and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania.
authors.forename: Valeria
authors.initials: V
authors.lastname: Tica
authors.affiliation: Center of Digestive Diseases and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania.
authors.forename: Viktoria
authors.initials: V
authors.lastname: Laszlo
authors.affiliation: Pathology Department, Hôtel-Dieu Hospital, Paris, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Mansuet-Lupo
authors.affiliation: Pathology Department, Hôtel-Dieu Hospital, Paris, France.
authors.forename: Dianne
authors.initials: D
authors.lastname: Damotte
authors.affiliation: Department of Thoracic Surgery, Vienna General Hospital, Vienna, Austria.
authors.forename: Walter
authors.initials: W
authors.lastname: Klepetko
authors.affiliation: Center of Digestive Diseases and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania.
authors.forename: Irinel
authors.initials: I
authors.lastname: Popescu
authors.affiliation: Center of Digestive Diseases and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania.
authors.forename: Jean Francois
authors.initials: JF
authors.lastname: Regnard
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000401:mortality / Q000473:pathology* / Q000628:therapy; D016000:Cluster Analysis; D003131:Combined Modality Therapy; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology* / Q000628:therapy; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008482:Mediastinum / Q000473:pathology*; D008875:Middle Aged; D008954:Models, Biological; D009367:Neoplasm Staging; D011379:Prognosis; D015398:Signal Transduction; D059467:Transcriptome; D016896:Treatment Outcome
keywords: Microarray; gene signature; non-small cell lung cancer