title: High-throughput hyperpolarized (13)C metabolic investigations using a multi-channel acquisition system.
abstract: Magnetic resonance imaging and spectroscopy of hyperpolarized (HP) compounds such as [1-(13)C]-pyruvate have shown tremendous potential for offering new insight into disease and response to therapy. New applications of this technology in clinical research and care will require extensive validation in cells and animal models, a process that may be limited by the high cost and modest throughput associated with dynamic nuclear polarization. Relatively wide spectral separation between [1-(13)C]-pyruvate and its chemical endpoints in vivo are conducive to simultaneous multi-sample measurements, even in the presence of a suboptimal global shim. Multi-channel acquisitions could conserve costs and accelerate experiments by allowing acquisition from multiple independent samples following a single dissolution. Unfortunately, many existing preclinical MRI systems are equipped with only a single channel for broadband acquisitions. In this work, we examine the feasibility of this concept using a broadband multi-channel digital receiver extension and detector arrays that allow concurrent measurement of dynamic spectroscopic data from ex vivo enzyme phantoms, in vitro anaplastic thyroid carcinoma cells, and in vivo in tumor-bearing mice. Throughput and the cost of consumables were improved by up to a factor of four. These preliminary results demonstrate the potential for efficient multi-sample studies employing hyperpolarized agents.
authors.affiliation: Department of Imaging Physics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Jaehyuk
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Imaging Physics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Marc S
authors.initials: MS
authors.lastname: Ramirez
authors.affiliation: Department of Imaging Physics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Walker
authors.affiliation: Department of Head & Neck Surgery, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Imaging Physics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Stacey
authors.initials: S
authors.lastname: Yi
authors.affiliation: Department of Otolaryngology, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Vlad C
authors.initials: VC
authors.lastname: Sandulache
authors.affiliation: Department of Head & Neck Surgery, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Stephen Y
authors.initials: SY
authors.lastname: Lai
authors.affiliation: Department of Imaging Physics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA. Electronic address: jbankson@mdanderson.org.
authors.forename: James A
authors.initials: JA
authors.lastname: Bankson
mesh_terms: D000703:Analog-Digital Conversion; D000818:Animals; D002247:Carbon Isotopes; D002277:Carcinoma / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D003365:Costs and Cost Analysis; D004798:Enzymes / Q000737:chemistry; D004867:Equipment Design; D005240:Feasibility Studies; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D008660:Metabolism; D051379:Mice; D019047:Phantoms, Imaging; D019289:Pyruvic Acid / Q000737:chemistry; D059629:Signal-To-Noise Ratio; D013964:Thyroid Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000473:pathology
chemical_list: D002247:Carbon Isotopes; D004798:Enzymes; D019289:Pyruvic Acid; D007770:L-Lactate Dehydrogenase
keywords: (13)C spectroscopy; Cancer; Coil array; Dynamic nuclear polarization; Dynamic spectroscopy; Hyperpolarization; Hyperpolarized pyruvate; Multichannel spectroscopy