title: The Effects of TiO2 Nanodot Films with RGD Immobilization on Light-Induced Cell Sheet Technology.
abstract: Cell sheet technology is a new strategy in tissue engineering which could be possible to implant into the body without a scaffold. In order to get an integrated cell sheet, a light-induced method via UV365 is used for cell sheet detachment from culture dishes. In this study, we investigated the possibility of cell detachment and growth efficiency on TiO2 nanodot films with RGD immobilization on light-induced cell sheet technology. Mouse calvaria-derived, preosteoblastic (MC3T3-E1) cells were cultured on TiO2 nanodot films with (TR) or without (TN) RGD immobilization. After cells were cultured with or without 5.5 mW/cm(2) UV365 illumination, cell morphology, cell viability, osteogenesis related RNA and protein expression, and cell detachment ability were compared, respectively. Light-induced cell detachment was possible when cells were cultured on TR samples. Also, cells cultured on TR samples showed better cell viability, alongside higher protein and RNA expression than on TN samples. This study provides a new biomaterial for light-induced cell/cell sheet harvesting.
authors.affiliation: The Affiliated Hospital of Stomatology, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, China.
authors.forename: Meng-Liu
authors.initials: ML
authors.lastname: Yu
authors.affiliation: The Affiliated Hospital of Stomatology, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, China.
authors.forename: Meng-Fei
authors.initials: MF
authors.lastname: Yu
authors.affiliation: The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, China.
authors.forename: Li-Qin
authors.initials: LQ
authors.lastname: Zhu
authors.affiliation: Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou, Zhejiang 310027, China.
authors.forename: Tian-Tian
authors.initials: TT
authors.lastname: Wang
authors.affiliation: The Affiliated Hospital of Stomatology, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: The Affiliated Hospital of Stomatology, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, China ; The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, China.
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Wang
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D055767:Immobilized Proteins / Q000737:chemistry*; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology; D010012:Osteogenesis; D023822:Tissue Engineering / Q000379:methods*; D014025:Titanium / Q000737:chemistry*
chemical_list: D055767:Immobilized Proteins; D009842:Oligopeptides; C009495:titanium dioxide; C047981:arginyl-glycyl-aspartic acid; D014025:Titanium