title: Search for Allergens from the Pollen Proteome of Sunflower (Helianthus annuus L.): A Major Sensitizer for Respiratory Allergy Patients.
abstract: BACKGROUND
  Respiratory allergy triggered by pollen allergens is increasing at an alarming rate worldwide. Sunflower pollen is thought to be an important source of inhalant allergens. Present study aims to identify the prevalence of sunflower pollinosis among the Indian allergic population and characterizes the pollen allergens using immuno-proteomic tools.
  
  
  METHODS
  Clinico-immunological tests were performed to understand the prevalence of sensitivity towards sunflower pollen among the atopic population. Sera from selected sunflower positive patients were used as probe to detect the IgE-reactive proteins from the one and two dimensional electrophoretic separated proteome of sunflower pollen. The antigenic nature of the sugar moiety of the glycoallergens was studied by meta-periodate modification of IgE-immunoblot. Finally, these allergens were identified by mass-spectrometry.
  
  
  RESULTS
  Prevalence of sunflower pollen sensitization was observed among 21% of the pollen allergic population and associated with elevated level of specific IgE and histamine in the sera of these patients. Immunoscreening of sunflower pollen proteome with patient sera detected seven IgE-reactive proteins with varying molecular weight and pI. Hierarchical clustering of 2D-immunoblot data highlighted three allergens characterized by a more frequent immuno-reactivity and increased levels of IgE antibodies in the sera of susceptible patients. These allergens were considered as the major allergens of sunflower pollen and were found to have their glycan moiety critical for inducing IgE response. Homology driven search of MS/MS data of these IgE-reactive proteins identified seven previously unreported allergens from sunflower pollen. Three major allergenic proteins were identified as two pectate lyases and a cysteine protease.
  
  
  CONCLUSIONS
  Novelty of the present report is the identification of a panel of seven sunflower pollen allergens for the first time at immuno-biochemical and proteomic level, which substantiated the clinical evidence of sunflower allergy. Further purification and recombinant expression of these allergens will improve component-resolved diagnosis and therapy of pollen allergy.
authors.affiliation: Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India.
authors.forename: Nandini
authors.initials: N
authors.lastname: Ghosh
authors.affiliation: Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India.
authors.forename: Gaurab
authors.initials: G
authors.lastname: Sircar
authors.affiliation: Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India.
authors.forename: Bodhisattwa
authors.initials: B
authors.lastname: Saha
authors.affiliation: Department of Allergy and Asthma, Belle Vue Clinic, Kolkata, India.
authors.forename: Naren
authors.initials: N
authors.lastname: Pandey
authors.affiliation: Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India.
authors.forename: Swati
authors.initials: S
authors.lastname: Gupta Bhattacharya
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology* / Q000378:metabolism; D052179:Antigens, Plant / Q000276:immunology* / Q000378:metabolism; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006368:Helianthus / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010940:Plant Proteins / Q000276:immunology* / Q000378:metabolism; D011058:Pollen / Q000276:immunology* / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012137:Respiratory System / Q000276:immunology / Q000378:metabolism; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis / Q000276:immunology; D012867:Skin / Q000276:immunology / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; D020543:Proteome; D007073:Immunoglobulin E