title: Inhibition of Viability of the Respiratory Epithelial Cells Using Functionalized Graphene Oxide.
abstract: The viability of A549 cells, a human lung carcinoma epithelial cell line, was evaluated after exposure to graphene oxide (GO) and its derivatives (dodecylamine GO (DA-GO), reduced GO (rGO), and sodium dodecyl sulfate rGO (SDS-rGO)). A decrease in the relative amounts of C-OH bonds and an increase in the number of C-C and C-N bonds in the C 1s spectra indicated that the reduction of GO to rGO and the surface functionalization of GO has taken place. The appearance of amine stretching bands, out-of-plane C-H stretching vibrations, and S = O stretching bands in the infrared spectra indicated the formation of DA-GO, rGO, and SDS-rGO, respectively. Low concentrations (3-25 µg/mL) of GO, rGO, and SDS-rGO were found to be mildly toxic, whereas DA-GO exhibited severe dose-dependent toxicity over the same concentration range. High concen- trations (50-400 µg/mL) of GO and all its derivatives resulted in severe toxicity to the A549 cells. It is believed that surface functionality strongly affects the viability of A549 cells.
authors.forename: Chang Seok
authors.initials: CS
authors.lastname: Park
authors.forename: Kyoung Soon
authors.initials: KS
authors.lastname: Choi
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Shin
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Kim
mesh_terms: D000588:Amines / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects*; D006108:Graphite / Q000009:adverse effects* / Q000737:chemistry*; D006801:Humans; D008168:Lung / Q000473:pathology*; D008958:Models, Molecular; D008968:Molecular Conformation; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000588:Amines; D010087:Oxides; D012967:Sodium Dodecyl Sulfate; D006108:Graphite; C003935:dodecylamine