title: A Novel Ilarvirus Is Associated with Privet Necrotic Ringspot Disease in the Southern United States.
abstract: Necrotic ringspot disease (NRSD) is a graft-transmissible disorder of privet (synonym ligustrum), originally reported from Florida and Louisiana more than 50 years ago. In this communication we report an isometric virus isolated from Japanese privet (Ligustrum japonicum) collected in the southern United States displaying symptoms resembling those of NRSD. In mechanical transmission tests, the virus induced systemic infections in several herbaceous hosts. Double-stranded RNA analysis showed a pattern resembling replicative forms of members of the family Bromoviridae. The genome organization along with phylogenetic analyses and serological tests revealed that the virus belongs to subgroup 1 of the genus Ilarvirus. Pairwise comparisons with recognized ilarviruses indicated that the virus is a distinct, and as yet, undescribed member in the taxon, for which we propose the name Privet ringspot virus (PrRSV). Furthermore, the near-perfect association of PrRSV infections with symptoms, and apparent absence of any other virus(es) in studied samples, strongly suggest an important role of this virus in the etiology of NRSD of privet in the southeastern United States.
authors.affiliation: First author: Institute for Genomics, Biocomputing and Biotechnology, Mississippi State, MS 39762; second author: Department of Plant Pathology, Division of Agriculture, University of Arkansas, Fayetteville 72701; third author: Institute for Imaging and Analytical Technologies, Mississippi State University, Mississippi State 39762; fourth author: Coastal Research and Extension Center, Mississippi State University, Biloxi 39532; and fifth author: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State 39762.
authors.forename: Nina
authors.initials: N
authors.lastname: Aboughanem-Sabanadzovic
authors.affiliation: First author: Institute for Genomics, Biocomputing and Biotechnology, Mississippi State, MS 39762; second author: Department of Plant Pathology, Division of Agriculture, University of Arkansas, Fayetteville 72701; third author: Institute for Imaging and Analytical Technologies, Mississippi State University, Mississippi State 39762; fourth author: Coastal Research and Extension Center, Mississippi State University, Biloxi 39532; and fifth author: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State 39762.
authors.forename: Ioannis E
authors.initials: IE
authors.lastname: Tzanetakis
authors.affiliation: First author: Institute for Genomics, Biocomputing and Biotechnology, Mississippi State, MS 39762; second author: Department of Plant Pathology, Division of Agriculture, University of Arkansas, Fayetteville 72701; third author: Institute for Imaging and Analytical Technologies, Mississippi State University, Mississippi State 39762; fourth author: Coastal Research and Extension Center, Mississippi State University, Biloxi 39532; and fifth author: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State 39762.
authors.forename: Amanda
authors.initials: A
authors.lastname: Lawrence
authors.affiliation: First author: Institute for Genomics, Biocomputing and Biotechnology, Mississippi State, MS 39762; second author: Department of Plant Pathology, Division of Agriculture, University of Arkansas, Fayetteville 72701; third author: Institute for Imaging and Analytical Technologies, Mississippi State University, Mississippi State 39762; fourth author: Coastal Research and Extension Center, Mississippi State University, Biloxi 39532; and fifth author: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State 39762.
authors.forename: Ronald C
authors.initials: RC
authors.lastname: Stephenson
authors.affiliation: First author: Institute for Genomics, Biocomputing and Biotechnology, Mississippi State, MS 39762; second author: Department of Plant Pathology, Division of Agriculture, University of Arkansas, Fayetteville 72701; third author: Institute for Imaging and Analytical Technologies, Mississippi State University, Mississippi State 39762; fourth author: Coastal Research and Extension Center, Mississippi State University, Biloxi 39532; and fifth author: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State 39762.
authors.forename: Sead
authors.initials: S
authors.lastname: Sabanadzovic
mesh_terms: D003001:Cloning, Molecular; D016679:Genome, Viral; D019179:Ilarvirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D031659:Ligustrum / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014481:United States
chemical_list: D012367:RNA, Viral