title: Enterovirus 71 2C Protein Inhibits NF-κB Activation by Binding to RelA(p65).
abstract: Viruses evolve multiple ways to interfere with NF-κB signaling, a key regulator of innate and adaptive immunity. Enterovirus 71 (EV71) is one of primary pathogens that cause hand-foot-mouth disease. Here, we identify RelA(p65) as a novel binding partner for EV71 2C protein from yeast two-hybrid screen. By interaction with IPT domain of p65, 2C reduces the formation of heterodimer p65/p50, the predominant form of NF-κB. We also show that picornavirus 2C family proteins inhibit NF-κB activation and associate with p65 and IKKβ. Our findings provide a novel mechanism how EV71 antagonizes innate immunity.
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100176, PR China.
authors.forename: Haiwei
authors.initials: H
authors.lastname: Du
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100176, PR China.
authors.forename: Peiqi
authors.initials: P
authors.lastname: Yin
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100176, PR China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Yang
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100176, PR China.
authors.forename: Leiliang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100176, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
authors.affiliation: Shanghai Municipal Center for Disease Control &Prevention, Shanghai 200336, China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Zhu
mesh_terms: D001665:Binding Sites / Q000235:genetics; D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D029821:Enterovirus A, Human / Q000378:metabolism*; D004789:Enzyme Activation; D057809:HEK293 Cells; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism; D057131:Immune Evasion / Q000276:immunology* / Q000502:physiology; D052002:NF-kappa B p50 Subunit / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics; D051996:Transcription Factor RelA / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D052002:NF-kappa B p50 Subunit; D051996:Transcription Factor RelA; D017361:Viral Nonstructural Proteins; D051550:I-kappa B Kinase; C076023:2C protein, viral