title: Evaluation of the impact of polyethylene microbeads ingestion in European sea bass (Dicentrarchus labrax) larvae.
abstract: Microplastics are present in marine habitats worldwide and may be ingested by low trophic organisms such as fish larvae, with uncertain physiological consequences. The present study aims at assessing the impact of polyethylene (PE 10-45 μM) microbeads ingestion in European sea bass (Dicentrarchus labrax) larvae. Fish were fed an inert diet including 0, 10(4) and 10(5) fluorescent microbeads per gram from 7 until 43 days post-hatching (dph). Microbeads were detected in the gastrointestinal tract in all fish fed diet incorporating PE. Our data revealed an efficient elimination of PE beads from the gut since no fluorescent was observed in the larvae after 48 h depuration. While the mortality rate increased significantly with the amount of microbeads scored per larvae at 14 and 20 dph, only ingestion of the highest concentration slightly impacted mortality rates. Larval growth and inflammatory response through Interleukine-1-beta (IL-1β) gene expression were not found to be affected while cytochrome-P450-1A1 (cyp1a1) expression level was significantly positively correlated with the number of microbeads scored per larva at 20 dph. Overall, these results suggest that ingestion of PE microbeads had limited impact on sea bass larvae possibly due to their high potential of egestion.
authors.affiliation: Ifremer, Centre de Bretagne, LEMAR UMR 6539, Plouzané, France. Electronic address: dmazurai@ifremer.fr.
authors.forename: D
authors.initials: D
authors.lastname: Mazurais
authors.affiliation: Ifremer, Channel and North Sea Fisheries Unit, Fisheries Laboratory, BP 699, Boulogne-sur-mer, 62321, France.
authors.forename: B
authors.initials: B
authors.lastname: Ernande
authors.affiliation: Ifremer, Centre de Bretagne, LEMAR UMR 6539, Plouzané, France.
authors.forename: P
authors.initials: P
authors.lastname: Quazuguel
authors.affiliation: Ifremer, Centre de Bretagne, LEMAR UMR 6539, Plouzané, France.
authors.forename: A
authors.initials: A
authors.lastname: Severe
authors.affiliation: Ifremer, Centre de Bretagne, LEMAR UMR 6539, Plouzané, France.
authors.forename: C
authors.initials: C
authors.lastname: Huelvan
authors.affiliation: Ifremer, Centre de Bretagne, LEMAR UMR 6539, Plouzané, France.
authors.forename: L
authors.initials: L
authors.lastname: Madec
authors.affiliation: Ifremer, Centre de Bretagne, LEMAR UMR 6539, Plouzané, France.
authors.forename: O
authors.initials: O
authors.lastname: Mouchel
authors.affiliation: CNRS, IUEM, LEMAR UMR 6539, Plouzané, France.
authors.forename: P
authors.initials: P
authors.lastname: Soudant
authors.affiliation: ILVO, Oostende, Belgium(3) ILVO, Oostende, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Robbens
authors.affiliation: Ifremer, Centre de Bretagne, LEMAR UMR 6539, Plouzané, France.
authors.forename: A
authors.initials: A
authors.lastname: Huvet
authors.affiliation: Ifremer, Centre de Bretagne, LEMAR UMR 6539, Plouzané, France.
authors.forename: J
authors.initials: J
authors.lastname: Zambonino-Infante
mesh_terms: D000824:Animal Nutritional Physiological Phenomena / Q000187:drug effects; D000818:Animals; D001492:Bass / Q000254:growth & development / Q000502:physiology*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D004063:Digestion; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D008136:Longevity / Q000187:drug effects; D008863:Microspheres; D020959:Polyethylene / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D029941:Fish Proteins; D053583:Interleukin-1beta; D014874:Water Pollutants, Chemical; D020959:Polyethylene; D019363:Cytochrome P-450 CYP1A1
keywords: Fish; Ingestion; Larvae; Microplastic; Survival