title: The use of date waste for lactic acid production by a fed-batch culture using Lactobacillus casei subsp. rhamnosus.
abstract: The production of lactic acid from date juice by Lactobacillus caseisubsp. rhamnosus in batch and fed-batch cultures has been investigated. The fed-batch culture system gave better results for lactic acid production and volumetric productivity. The aim of this work is to determine the effects of the feeding rate and the concentration of the feeding medium containing date juice glucose on the cell growth, the consumption of glucose and the lactic acid production by Lactobacillus casei subsp. rhamnosus in fed-batch cultures. For this study, two concentrations of the feeding medium (62 and 100 g/L of date juice glucose) were tested at different feeding rates (18, 22, 33, 75 and 150 mL/h). The highest volumetric productivity (1.3 g/L.h) and lactic acid yield (1.7 g/g) were obtained at a feeding rate of 33 mL/h and a date juice glucose concentration of 62 g/L in the feeding medium. As a result, most of the date juice glucose was completely utilised (residual glucose 1 g/L), and a maximum lactic acid production level (89.2 g/L) was obtained.
authors.affiliation: Laboratory of Applied Microbiology, Ferhat Abbas University, Setif, Algeria .
authors.forename: Aicha
authors.initials: A
authors.lastname: Nancib
authors.affiliation: Laboratory of Applied Microbiology, Ferhat Abbas University, Setif, Algeria .
authors.forename: Nabil
authors.initials: N
authors.lastname: Nancib
authors.affiliation: Laboratory of Applied Microbiology, Ferhat Abbas University, Setif, Algeria .
authors.forename: Abdelhafid
authors.initials: A
authors.lastname: Boubendir
authors.affiliation: Laboratory Reactions and Chemical Engineering, University of Lorraine, Vandoeuvre Cedex, France .
authors.forename: Joseph
authors.initials: J
authors.lastname: Boudrant
mesh_terms: D061249:Batch Cell Culture Techniques; D005285:Fermentation; D019344:Lactic Acid / Q000378:metabolism*; D007780:Lacticaseibacillus casei / Q000378:metabolism*; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism*; D065749:Phoeniceae / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D019344:Lactic Acid
keywords: Lactobacillus casei subsp. rhamnosus; date juice; fed-batch culture; lactic acid