title: Preliminary Study: Prominent miRNAs of Breast Malignant Tissues Compared to Normal Tissues in Turkish Patients with Breast Cancer.
abstract: miRNA involvement has been observed in almost every type of cancer, including breast cancer. The etiology of abnormal expression of miRNAs in cancer is still not clearly understood. In order to obtain insight into miRNA de-regulation in breast cancer, we analyzed expression levels of five breast cancer-related miRNAs, miRNA21, miRNA155, miRNA19a, miRNA17-5p and let7a miRNA, in both malignant and neighboring non-tumoral paraffin-embedded tissues of 47 patients with invasive ductal breast cancer. The targeted miRNAs, and a reference snRNA, U6, were analyzed by real-time polymerase chain reaction. let7a Levels were significantly lower in patients with lymphatic invasion than in those without (p=0.047). miR21 was down-regulated in 93.3% of patients with necrosis [p=0.017 (Fisher's exact test (FE))], while at least one oncogenic miRNA was up-regulated in 87.3% of the patients with invasive ductal carcinoma [p=0.009 (FE)]. In addition, tumor-suppressor miRNA was down-regulated or unaltered in 65.8% of the patients with tumor grade 2 or 3 and in all with grade 1 [p=0.047 (FE)]. Based on this preliminary study, we suggest that these miRNAs, especially let7a and miRNA21, might be useful markers in follow-up of breast cancer and in prognosis.
authors.affiliation: Department of Pathology, Cerrahpasa Medical School, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Tulin
authors.initials: T
authors.lastname: Ozturk
authors.affiliation: Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Kucukhuseyin
authors.affiliation: Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Allison Pinar
authors.initials: AP
authors.lastname: Eronat
authors.affiliation: Department of Molecular Biology and Genetics, Istanbul Technical University, Istanbul, Turkey.
authors.forename: Mete Bora
authors.initials: MB
authors.lastname: Tuzuner
authors.affiliation: Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Aynur
authors.initials: A
authors.lastname: Daglar-Aday
authors.affiliation: Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Neslihan
authors.initials: N
authors.lastname: Saygili
authors.affiliation: Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey Department of Molecular Biology and Genetics, Istanbul Technical University, Istanbul, Turkey.
authors.forename: Halil Ibrahim
authors.initials: HI
authors.lastname: Kisakesen
authors.affiliation: Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Seyhan
authors.affiliation: Department of General Surgery, Cerrahpasa Medical School, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Velidedeoğlu
authors.affiliation: Department of Pathology, Cerrahpasa Medical School, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Zerrin
authors.initials: Z
authors.lastname: Calay
authors.affiliation: Department of Pathology, Cerrahpasa Medical School, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Şennur
authors.initials: Ş
authors.lastname: Ilvan
authors.affiliation: Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Hülya
authors.initials: H
authors.lastname: Yilmaz-Aydoğan
authors.affiliation: Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey droguzozturk@gmail.com.
authors.forename: Oguz
authors.initials: O
authors.lastname: Ozturk
authors.affiliation: Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Turgar
authors.initials: T
authors.lastname: Isbir
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D014421:Turkey
chemical_list: D035683:MicroRNAs
keywords: Breast cancer; let7a; miRNA155; miRNA17-5p; miRNA19a; miRNA21; paraffin-embedded tissue