title: Tectonics of a K⁺ channel: The importance of the N-terminus for channel gating.
abstract: The small K⁺ channel Kcv represents the pore module of complex potassium channels. It was found that its gating can be modified by sensor domains, which are N-terminally coupled to the pore. This implies that the short N-terminus of the channel can transmit conformational changes from upstream sensors to the channel gates. To understand the functional role of the N-terminus in the context of the entire channel protein, we apply combinatorial screening of the mechanical coupling and long-range interactions in the Kcv potassium channel by reduced molecular models. The dynamics and mechanical connections in the channel complex show that the N-terminus is indeed mechanically connected to the pore domain. This includes a long rang coupling to the pore and the inner and outer transmembrane domains. Since the latter domains host the two gates of the channel, the data support the hypothesis that mechanical perturbation of the N-terminus can be transmitted to the channel gates. This effect is solely determined by the topology of the channel; sequence details only have an implicit effect on the coarse-grained dynamics via the fold and not through biochemical details at a smaller scale. This observation has important implications for engineering of synthetic channels on the basis of a K⁺ channel pore.
authors.affiliation: Computational Biology & Simulation Group, Dept. of Biology, TU Darmstadt, Germany; Physikalische Chemie III, TU Dortmund, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Hoffgaard
authors.affiliation: Physikalische Chemie III, TU Dortmund, Germany.
authors.forename: S M
authors.initials: SM
authors.lastname: Kast
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Milano e Istituto di Biofisica, CNR, Milano, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Moroni
authors.affiliation: Membrane Biophysics Group, Dept. of Biology, TU Darmstadt, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Thiel
authors.affiliation: Computational Biology & Simulation Group, Dept. of Biology, TU Darmstadt, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Hamacher
mesh_terms: D015640:Ion Channel Gating; D009154:Mutation; D015221:Potassium Channels / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D011487:Protein Conformation
chemical_list: D015221:Potassium Channels
keywords: Computational biophysics; Ion channels; Kcv; Reduced molecular models; Structure-function correlates