title: A Pimarane Diterpene and Cytotoxic Angucyclines from a Marine-Derived Micromonospora sp. in Vietnam's East Sea.
abstract: A screening of our actinomycete fraction library against the NCI-60 SKOV3 human tumor cell line led to the isolation of isopimara-2-one-3-ol-8,15-diene (1), lagumycin B (2), dehydrorabelomycin (3), phenanthroviridone (4), and WS-5995 A (5). These secondary metabolites were produced by a Micromonospora sp. isolated from sediment collected off the Cát Bà peninsula in the East Sea of Vietnam. Compound 1 is a novel Δ(8,9)-pimarane diterpene, representing one of approximately 20 actinomycete-produced diterpenes reported to date, while compound 2 is an angucycline antibiotic that has yet to receive formal characterization. The structures of 1 and 2 were elucidated by combined NMR and MS analysis and the absolute configuration of 1 was assigned by analysis of NOESY NMR and CD spectroscopic data. Compounds 2-5 exhibited varying degrees of cytotoxicity against a panel of cancerous and non-cancerous cell lines. Overall, this study highlights our collaborative efforts to discover novel biologically active molecules from the large, underexplored, and biodiversity-rich waters of Vietnam's East Sea.
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street (MC 781), Room 539, Chicago, IL 60612-7231, USA. mmullo2@uic.edu.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Mullowney
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street (MC 781), Room 539, Chicago, IL 60612-7231, USA. eohain2@uic.edu.|Center for Biomolecular Sciences, College of Pharmacy, University of Illinois at Chicago, Molecular Biology Research Building, 900 South Ashland Avenue (MC 870), Room 3150, Chicago, IL 60607-7173, USA. eohain2@uic.edu.
authors.forename: Eoghainín
authors.initials: E
authors.lastname: Ó hAinmhire
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street (MC 781), Room 539, Chicago, IL 60612-7231, USA. urszula.tanouye@gmail.com.
authors.forename: Urszula
authors.initials: U
authors.lastname: Tanouye
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street (MC 781), Room 539, Chicago, IL 60612-7231, USA. joannab@uic.edu.|Center for Biomolecular Sciences, College of Pharmacy, University of Illinois at Chicago, Molecular Biology Research Building, 900 South Ashland Avenue (MC 870), Room 3150, Chicago, IL 60607-7173, USA. joannab@uic.edu.
authors.forename: Joanna E
authors.initials: JE
authors.lastname: Burdette
authors.affiliation: Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoàng Quốc Việt, Cầu Giấy, Hà Nội 10000, Vietnam. phamvc@yahoo.com.
authors.forename: Van Cuong
authors.initials: VC
authors.lastname: Pham
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street (MC 781), Room 539, Chicago, IL 60612-7231, USA. btmurphy@uic.edu.|Center for Biomolecular Sciences, College of Pharmacy, University of Illinois at Chicago, Molecular Biology Research Building, 900 South Ashland Avenue (MC 870), Room 3150, Chicago, IL 60607-7173, USA. btmurphy@uic.edu.
authors.forename: Brian T
authors.initials: BT
authors.lastname: Murphy
mesh_terms: D045784:Abietanes / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008847:Micromonospora / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D009792:Oceans and Seas; D010051:Ovarian Neoplasms / Q000188:drug therapy; D014744:Vietnam
chemical_list: D045784:Abietanes; D000970:Antineoplastic Agents
keywords: Micromonospora; Vietnam; actinomycete; diterpene; marine; ovarian cancer