title: Decreased expression of ectonucleotidase E-NPP1 in leukocytes from subjects with severe asthma exacerbation.
abstract: Several studies suggest that ATP and related nucleotides play a role in the pathophysiology of asthma. However, the functionality of ectonucleotidases in this disease has been scantly investigated. We studied total ectonucleotidase activity in leukocytes from patients suffering from asthma exacerbation and explored the expression of E-NTPDase 1, 2, 3, and 8, and E-NPP1, 2, and 3, in their polymorphonuclear cells by immunofluorescence and qPCR. Leukocytes from patients with mild or moderate asthma exacerbation had similar ectonucleotidase activity than leukocytes from healthy subjects, while in patients with severe asthma exacerbation, this activity was lower. Of the ectonucleotidases studied, only E-NPP1 displayed diminished immunofluorescence and a significant decrease in its mRNA expression, both in patients with severe asthma exacerbation. This reduced E-NPP1 expression could be responsible for increased amounts of ATP or other nucleotides, capable of worsening asthma exacerbation, and warranting further investigation.
authors.affiliation: Departamento de Farmacología, Facultad de Medicina, Universidad Nacional Autónoma de México, México DF, México.
authors.forename: L M
authors.initials: LM
authors.lastname: Montaño
authors.affiliation: Departamento de Investigación en Hiperreactividad Bronquial, Instituto Nacional de Enfermedades Respiratorias, México DF, México.
authors.forename: M H
authors.initials: MH
authors.lastname: Vargas
authors.affiliation: Departamento de Embriología, Facultad de Medicina, Universidad Nacional Autónoma de México, México DF, México.
authors.forename: V
authors.initials: V
authors.lastname: Díaz-Hernández
authors.affiliation: Departamento de Farmacología, Facultad de Medicina, Universidad Nacional Autónoma de México, México DF, México.|Departamento de Embriología, Facultad de Medicina, Universidad Nacional Autónoma de México, México DF, México.
authors.forename: M
authors.initials: M
authors.lastname: De Ita
authors.affiliation: Departamento de Farmacología, Facultad de Medicina, Universidad Nacional Autónoma de México, México DF, México.
authors.forename: R
authors.initials: R
authors.lastname: Kazakova
authors.affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, San Luis Potosí (SLP), México.
authors.forename: C
authors.initials: C
authors.lastname: Barajas-López
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001249:Asthma / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D018450:Disease Progression; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000235:genetics* / Q000378:metabolism; D011755:Pyrophosphatases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D000255:Adenosine Triphosphate; D010727:Phosphoric Diester Hydrolases; C065962:ectonucleotide pyrophosphatase phosphodiesterase 1; D011755:Pyrophosphatases
keywords: E-NPP1; adenosine 5′-triphosphate; ectonucleotidase; severe asthma exacerbation