title: Notch signaling regulates M2 type macrophage polarization during the development of proliferative vitreoretinopathy.
abstract: Macrophages play an important role in the pathogenesis of proliferative vitreoretinopathy (PVR). M2 macrophages can promote tissue remodeling and repair. In this study, CD206 positive M2 type macrophages were found in preretinal fibrous membranes of the mouse model of PVR induced by the intravitreal injection of retinal pigment epithelial (RPE) cells. Notch signaling determines M2 macrophage polarization. The specific inhibition of Notch signaling pathway by the intravitreal injection of γ-secretase inhibitor DAPT attenuated RPE cells-induced PVR formation as demonstrated by the decreased expression of α-SMA, and inhibited M2 type macrophage infiltation as demonstrated by the decreased expression of Arg-1. Notch signaling may modulate PVR formation by regulating M2 type macrophage polarization.
authors.affiliation: Shandong Eye Hospital, Shandong Eye Institute, Shandong Academy of Medical Sciences, Jinan, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Shandong Eye Hospital, Shandong Eye Institute, Shandong Academy of Medical Sciences, Jinan, China.
authors.forename: Gongqiang
authors.initials: G
authors.lastname: Yuan
authors.affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China.
authors.forename: Muchen
authors.initials: M
authors.lastname: Dong
authors.affiliation: Shandong Eye Hospital, Shandong Eye Institute, Shandong Academy of Medical Sciences, Jinan, China; State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China. Electronic address: weiyunshi@163.com.
authors.forename: Weiyun
authors.initials: W
authors.lastname: Shi
mesh_terms: D000199:Actins / Q000096:biosynthesis; D053829:Amyloid Precursor Protein Secretases / Q000037:antagonists & inhibitors*; D000818:Animals; D001119:Arginase / Q000096:biosynthesis; D016764:Cell Polarity / Q000276:immunology; D004151:Dipeptides / Q000494:pharmacology*; D058449:Intravitreal Injections; D037181:Lectins, C-Type / Q000378:metabolism; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D011956:Receptors, Cell Surface / Q000378:metabolism; D051880:Receptors, Notch / Q000276:immunology*; D055213:Retinal Pigment Epithelium / Q000166:cytology; D015398:Signal Transduction; D018630:Vitreoretinopathy, Proliferative / Q000276:immunology*
chemical_list: C558396:Acta2 protein, mouse; D000199:Actins; D004151:Dipeptides; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; C419410:N-(N-(3,5-difluorophenacetyl)alanyl)phenylglycine tert-butyl ester; D011956:Receptors, Cell Surface; D051880:Receptors, Notch; D053829:Amyloid Precursor Protein Secretases; C544448:Arg1 protein, mouse; D001119:Arginase
keywords: Macrophages; Notch signaling; Proliferative vitreoretinopathy; Retinal pigment epithelial cells; Wound healing