title: Determination of Nine Fungicides in Grape and Wine Using QuEChERS Extraction and LC/MS/MS Analysis.
abstract: An analytic procedure was developed for the determination of the fungicides ametoctradin, boscalid, cyazofamid, dimethomorph, fenhexamid, kresoxim-methyl, mepanipyrim, metrafenone, and pyraclostrobin in grape and wine. A modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) procedure was used for the extraction. Analysis of the extract was performed by LC/triple quadrupole-MS/MS. A Poroshell 120 EC-C18 column was used with a programmed gradient mobile phase consisting of (A) acetonitrile containing 0.1% formic acid and (B) water containing 0.1% formic acid and 2 mM ammonium formate. The acceptance criteria for the method were those proposed in the SANCO guide. The method was linear for the range of concentration studied (5-100 μg/L), and R2 values were higher than 0.998 and RSD values below 18%. Recovery was over 73.2% in grape and 76.7% in wines, and there was no case of more than 100% recovery. The recovery RSDs in reproducibility conditions were below 17.13% in grape and 15.6% in wines.
authors.forename: Gracia
authors.initials: G
authors.lastname: Martínez
authors.forename: Ascensión
authors.initials: A
authors.lastname: Morales
authors.forename: Alba
authors.initials: A
authors.lastname: Maestro
authors.forename: Sandra
authors.initials: S
authors.lastname: Cermeño
authors.forename: José
authors.initials: J
authors.lastname: Oliva
authors.forename: Alberto
authors.initials: A
authors.lastname: Barba
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D005659:Fungicides, Industrial / Q000032:analysis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D005659:Fungicides, Industrial