title: Carbon and Nitrogen Sources Influence Tricalcium Phosphate Solubilization and Extracellular Phosphatase Activity by Talaromyces flavus.
abstract: The aim of this work was to study phosphate (P) solubilization (and the processes involved in this event) by Talaromyces flavus (BAFC 3125) as a function of carbon and/or nitrogen sources. P solubilization was evaluated in NBRIP media supplemented with different carbon (glucose, sorbitol, sucrose, and fructose) and nitrogen (L-asparagine, urea, ammonium sulfate (AS), and ammonium nitrate (AN) combinations. The highest P solubilization was related to the highest organic acid production (especially gluconic acid) and pH drop for those treatments where glucose was present. Also P solubilization was higher when an inorganic nitrogen source was supplemented to the media when compared to an organic one. Although not being present an organic P source, phosphatase activity was observed. This shows that P mineralization and P solubilization can occur simultaneously, and that P mineralization is not induced by the enzyme substrate. The combination that showed highest P solubilization was for AN-glucose. The highest acid phosphatase activity was for AS-fructose, while for alkaline phosphatase were for AS-fructose and AN-fructose. Acid phosphatase activity was higher than alkaline. P solubilization and phosphatase activity (acid and alkaline) were influenced by the different carbon-nitrogen combinations. A better understanding of phosphate-solubilizing fungi could bring a better use of soil P.
authors.affiliation: Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, 4th floor Ciudad Universitaria, 1428, Buenos Aires, Argentina.
authors.forename: P J
authors.initials: PJ
authors.lastname: Stefanoni Rubio
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, 4th floor Ciudad Universitaria, 1428, Buenos Aires, Argentina.
authors.forename: M S
authors.initials: MS
authors.lastname: Godoy
authors.affiliation: Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, 4th floor Ciudad Universitaria, 1428, Buenos Aires, Argentina.
authors.forename: I F
authors.initials: IF
authors.lastname: Della Mónica
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, 4th floor Ciudad Universitaria, 1428, Buenos Aires, Argentina.
authors.forename: M J
authors.initials: MJ
authors.lastname: Pettinari
authors.affiliation: Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, 4th floor Ciudad Universitaria, 1428, Buenos Aires, Argentina.
authors.forename: A M
authors.initials: AM
authors.lastname: Godeas
authors.affiliation: Departamento de Botánica, INIBIOMA (CONICET-UNComahue), Quintral 1250, 8400, San Carlos de Bariloche, Río Negro, Argentina. scervino@bg.fcen.uba.ar.
authors.forename: J M
authors.initials: JM
authors.lastname: Scervino
mesh_terms: D002130:Calcium Phosphates / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D009584:Nitrogen / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D032901:Talaromyces / Q000201:enzymology* / Q000378:metabolism*
chemical_list: D002130:Calcium Phosphates; D003470:Culture Media; D002244:Carbon; D010744:Phosphoric Monoester Hydrolases; C018392:tricalcium phosphate; D009584:Nitrogen