title: Transcriptome Analysis Revealed the Embryo-Induced Gene Expression Patterns in the Endometrium from Meishan and Yorkshire Pigs.
abstract: The expression patterns in Meishan- and Yorkshire-derived endometrium during early (gestational day 15) and mid-gestation (gestational days 26 and 50) were investigated, respectively. Totally, 689 and 1649 annotated genes were identified to be differentially expressed in Meishan and Yorkshire endometrium during the three gestational stages, respectively. Hierarchical clustering analysis identified that, of the annotated differentially expressed genes (DEGs), 73 DEGs were unique to Meishan endometrium, 536 DEGs were unique to Yorkshire endometrium, and 228 DEGs were common in Meishan and Yorkshire endometriums. Subsequently, DEGs in each of the three types of expression patterns were grouped into four distinct categories according to the similarities in their temporal expression patterns. The expression patterns identified from the microarray analysis were validated by quantitative RT-PCR. The functional enrichment analysis revealed that the common DEGs were enriched in pathways of steroid metabolic process and regulation of retinoic acid receptor signaling. These unique DEGs in Meishan endometrium were involved in cell cycle and adherens junction. The DEGs unique to Yorkshire endometrium were associated with regulation of Rho protein signal transduction, maternal placenta development and cell proliferation. This study revealed the different gene expression patterns or pathways related to the endometrium remodeling in Meishan and Yorkshire pigs, respectively. These unique DEGs in either Meishan or Yorkshire endometriums may contribute to the divergence of the endometrium environment in the two pig breeds.
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry and the Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China. huangjiangnan@webmail.hzau.edu.cn.|Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China. huangjiangnan@webmail.hzau.edu.cn.
authors.forename: Jiangnan
authors.initials: J
authors.lastname: Huang
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry and the Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China. lrz_2003@163.com.
authors.forename: Ruize
authors.initials: R
authors.lastname: Liu
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry and the Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China. sulijie@webmail.hzau.edu.cn.
authors.forename: Lijie
authors.initials: L
authors.lastname: Su
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry and the Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China. xiaoqian2013@sjtu.edu.cn.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry and the Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China. yumei@mail.hzau.edu.cn.
authors.forename: Mei
authors.initials: M
authors.lastname: Yu
mesh_terms: D000818:Animals; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D011247:Pregnancy; D013552:Swine / Q000196:embryology* / Q000235:genetics*; D059467:Transcriptome
keywords: Meishan and Yorkshire pigs; endometrium; gene expression pattern; microarray; pig