title: "Parallel factor analysis of multi-excitation ultraviolet resonance Raman spectra for protein secondary structure determination".
abstract: Protein secondary structural analysis is important for understanding the relationship between protein structure and function, or more importantly how changes in structure relate to loss of function. The structurally sensitive protein vibrational modes (amide I, II, III and S) in deep-ultraviolet resonance Raman (DUVRR) spectra resulting from the backbone C-O and N-H vibrations make DUVRR a potentially powerful tool for studying secondary structure changes. Experimental studies reveal that the position and intensity of the four amide modes in DUVRR spectra of proteins are largely correlated with the varying fractions of α-helix, β-sheet and disordered structural content of proteins. Employing multivariate calibration methods and DUVRR spectra of globular proteins with varying structural compositions, the secondary structure of a protein with unknown structure can be predicted. A disadvantage of multivariate calibration methods is the requirement of known concentration or spectral profiles. Second-order curve resolution methods, such as parallel factor analysis (PARAFAC), do not have such a requirement due to the "second-order advantage." An exceptional feature of DUVRR spectroscopy is that DUVRR spectra are linearly dependent on both excitation wavelength and secondary structure composition. Thus, higher order data can be created by combining protein DUVRR spectra of several proteins collected at multiple excitation wavelengths to give multi-excitation ultraviolet resonance Raman data (ME-UVRR). PARAFAC has been used to analyze ME-UVRR data of nine proteins to resolve the pure spectral, excitation and compositional profiles. A three factor model with non-negativity constraints produced three unique factors that were correlated with the relative abundance of helical, β-sheet and poly-proline II dihedral angles. This is the first empirical evidence that the typically resolved "disordered" spectrum represents the better defined poly-proline II type structure.
authors.affiliation: University of Missouri, Department of Chemistry, 601 S. College Avenue, Columbia, MO 65211, USA.
authors.forename: Olayinka O
authors.initials: OO
authors.lastname: Oshokoya
authors.affiliation: University of Missouri, Department of Chemistry, 601 S. College Avenue, Columbia, MO 65211, USA. Electronic address: jijir@missouri.edu.
authors.forename: Renee D
authors.initials: RD
authors.lastname: JiJi
mesh_terms: D000465:Algorithms; D000596:Amino Acids / Q000737:chemistry; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman
chemical_list: D000596:Amino Acids; D011506:Proteins
keywords: Multiway; Parallel factor analysis; Protein secondary structure; Trilinear; Ultraviolet resonance Raman