title: LYAR promotes colorectal cancer cell mobility by activating galectin-1 expression.
abstract: Colorectal cancer (CRC) is one of the leading causes of cancer-related death worldwide. However, the molecular mechanisms of CRC pathogenesis are not fully understood. In this study, we report the characterization of LYAR (Ly-1 antibody reactive clone) as a key regulator of the migration and invasion of human CRC cells. Immunohistochemistry analysis demonstrated that LYAR is expressed at a higher level in metastatic CRC tissues. We found that LYAR promoted the migratory and invasive capabilities of CRC cells. Gene expression profile analysis of CRC cells showed that LGALS1, which encodes the galectin-1 protein, was a potential target of LYAR. The ChIP assay and gene reporter assays indicated that LYAR directly bound to the LGALS1 promoter. The ectopic expression of galectin-1 partially restored the mobile potential of LYAR knocked-down cells, which suggests that galectin-1 contributed to the LYAR-promoted cell migration and invasion of CRC cells. Thus, this study revealed a novel mechanism by which the transcription factor LYAR may promote tumor cell migration and invasion by upregulating galectin-1 gene expression in CRC.
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China.|Anhui Research Institute for Family Planning, Anhui Research Center for Population and Birth Control, Hefei, 230031, China.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Wu
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China.
authors.forename: Zhuchen
authors.initials: Z
authors.lastname: Li
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Wu
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China.
authors.forename: Min
authors.initials: M
authors.lastname: Nie
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Huang
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Ju
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Ma
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China.
authors.forename: Renxiang
authors.initials: R
authors.lastname: Tan
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zen
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China.
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: Anhui Research Institute for Family Planning, Anhui Research Center for Population and Birth Control, Hefei, 230031, China.
authors.forename: Keqin
authors.initials: K
authors.lastname: Fu
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.
authors.forename: Yu-Gen
authors.initials: YG
authors.lastname: Chen
authors.affiliation: The State Key Laboratory of Molecular Oncology, Cancer Hospital and Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100021, China.
authors.forename: Ming-Rong
authors.initials: MR
authors.lastname: Wang
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhao
mesh_terms: D001665:Binding Sites; D002465:Cell Movement; D047369:Chromatin Immunoprecipitation; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D037483:Galectin 1 / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D045325:HCT116 Cells; D006801:Humans; D007150:Immunohistochemistry; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D014162:Transfection; D015854:Up-Regulation
chemical_list: D004268:DNA-Binding Proteins; D037483:Galectin 1; C445499:LGALS1 protein, human; C000592018:LYAR protein, human; D009687:Nuclear Proteins
keywords: LYAR; cell migration and invasion; colorectal cancer; galectin-1