title: The Murray collection of pre-antibiotic era Enterobacteriacae: a unique research resource.
abstract: Studies of historical isolates inform on the evolution and emergence of important pathogens and phenotypes, including antimicrobial resistance. Crucial to studying antimicrobial resistance are isolates that predate the widespread clinical use of antimicrobials. The Murray collection of several hundred bacterial strains of pre-antibiotic era Enterobacteriaceae is an invaluable resource of historical strains from important pathogen groups. Studies performed on the Collection to date merely exemplify its potential, which will only be realised through the continued effort of many scientific groups. To enable that aim, we announce the public availability of the Murray collection through the National Collection of Type Cultures, and present associated metadata with whole genome sequence data for over half of the strains. Using this information we verify the metadata for the collection with regard to subgroup designations, equivalence groupings and plasmid content. We also present genomic analyses of population structure and determinants of mobilisable antimicrobial resistance to aid strain selection in future studies. This represents an invaluable public resource for the study of these important pathogen groups and the emergence and evolution of antimicrobial resistance.
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK.
authors.forename: Kate S
authors.initials: KS
authors.lastname: Baker
authors.affiliation: National Collection of Type Cultures, Public Health England, Porton Down, Salisbury, SP4 0JG, UK.
authors.forename: Edward
authors.initials: E
authors.lastname: Burnett
authors.affiliation: National Collection of Type Cultures, Public Health England, Porton Down, Salisbury, SP4 0JG, UK.
authors.forename: Hannah
authors.initials: H
authors.lastname: McGregor
authors.affiliation: National Collection of Type Cultures, Public Health England, Porton Down, Salisbury, SP4 0JG, UK.
authors.forename: Ana
authors.initials: A
authors.lastname: Deheer-Graham
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK.
authors.forename: Christine
authors.initials: C
authors.lastname: Boinett
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK.
authors.forename: Gemma C
authors.initials: GC
authors.lastname: Langridge
authors.affiliation: University of Queensland, St Lucia, QLD, 4072, Australia.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Wailan
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK.
authors.forename: Amy K
authors.initials: AK
authors.lastname: Cain
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK.|Department of Pathogen Molecular Biology, the London School of Hygiene and Tropical Medicine, London, UK.
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Thomson
authors.affiliation: National Collection of Type Cultures, Public Health England, Porton Down, Salisbury, SP4 0JG, UK.
authors.forename: Julie E
authors.initials: JE
authors.lastname: Russell
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK. parkhill@sanger.ac.uk.
authors.forename: Julian
authors.initials: J
authors.lastname: Parkhill
mesh_terms: D000900:Anti-Bacterial Agents; D018070:Biological Specimen Banks; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000235:genetics; D056186:Metagenomics
chemical_list: D000900:Anti-Bacterial Agents