title: Expression, purification, and breast cancer cell inhibiting effect of recombinant human lactoferrin C-lobe.
abstract: Lactoferrin (LTF), a multifunctional glycoprotein of the transferrin family mainly found in exotic secretions in mammals, is an important defense molecule against not only microbial invasion but also tumors. It folds into two globular domains (N- and C-lobes) each containing an iron-binding site. The cationic antimicrobial peptide in N-lobe is known to exert anti-tumor effect via a non-receptor-mediated pathway. However, whether LTF C-lobe also contributes to its anti-tumor activity remains to be investigated. In this study, a human LTF fragment (amino acid residues 343-682) covering the C-lobe was expressed with a histidine tag in E. coli and the purified polypeptide refolded through a series of buffer changing procedure. The resultant recombinant protein caused significant growth arrest of breast carcinoma cells MDA-MB-231 in a dose- and time-dependent manner, evidently via induction of apoptosis of the cell. Our data suggest a positive role for the C-lobe of human LTF in controlling tumors in vitro.
authors.affiliation: a Institute of Biology and Medical Sciences, School of Biology and Basic Medical Science, Soochow University , Suzhou , China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Hu
authors.affiliation: a Institute of Biology and Medical Sciences, School of Biology and Basic Medical Science, Soochow University , Suzhou , China.
authors.forename: Chen-Hui
authors.initials: CH
authors.lastname: Gao
authors.affiliation: a Institute of Biology and Medical Sciences, School of Biology and Basic Medical Science, Soochow University , Suzhou , China.
authors.forename: Chao
authors.initials: C
authors.lastname: Hong
authors.affiliation: a Institute of Biology and Medical Sciences, School of Biology and Basic Medical Science, Soochow University , Suzhou , China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zhong
authors.affiliation: a Institute of Biology and Medical Sciences, School of Biology and Basic Medical Science, Soochow University , Suzhou , China.
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Dong
authors.affiliation: a Institute of Biology and Medical Sciences, School of Biology and Basic Medical Science, Soochow University , Suzhou , China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Gao
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006639:Histidine / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000378:metabolism; D007781:Lactoferrin / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D009842:Oligopeptides / Q000378:metabolism; D010455:Peptides / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology
chemical_list: D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents; C471213:His-His-His-His-His-His; C490098:LTF protein, human; D009842:Oligopeptides; D010455:Peptides; D011993:Recombinant Fusion Proteins; D006639:Histidine; D007501:Iron; D007781:Lactoferrin
keywords: cancer; lactoferrin; recombinant protein