title: Comparison of ASCs and BMSCs combined with atelocollagen for vocal fold scar regeneration.
abstract: Vocal fold scar remains a therapeutic challenge. Mesenchymal stromal cells (MSCs) are promising tools for regenerative medicine. Nevertheless, few in vivo studies have directly compared various sources of MSCs. The aim of this study was to investigate the therapeutic potential of adipose-derived stromal cells (ASCs) in comparison with bone marrow-derived stromal cells (BMSCs) for vocal fold regeneration.
  
  
  
  Prospective animal experiments with controls.
  
  
  
  Two months after stripping of the lamina propria, 18 beagles were divided into four implantation groups: atelocollagen alone (collagen group), atelocollagen with BMSCs (BMSC-collagen), atelocollagen with ASCs (ASC-collagen), or a sham-treated group. One or 6 months after implantation, vibratory and histological examinations were performed.
  
  
  
  Mucosal vibration was significantly improved in both of the MSC-implanted groups compared with the sham-treated group, whereas only the ASC-collagen group showed a significantly smaller glottal gap than the collagen group. Moreover, in the ASC-collagen group, a significant reduction of collagen density was observed compared to the sham-treated group, and there was a trend for better restoration of hyaluronic acid (HA). Implanted MSCs were detected 1 month postimplantation; however, none survived 6 months postimplantation.
  
  
  
  Although implantation of an atelocollagen sponge and ASCs into vocal fold scars induced vibratory recovery comparable to that of BMSCs, ASCs might have more potential in terms of restoration of HA and suppression of excessive collagen deposition.
  
  
  
  NA Laryngoscope, 126:1143-1150, 2016.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.|Department of Otolaryngology, New York University School of Medicine, New York, New York.
authors.forename: Nao
authors.initials: N
authors.lastname: Hiwatashi
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hirano
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Suzuki
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Kawai
authors.affiliation: Department of Otolaryngology, Vanderbilt University, Nashville, Tennessee.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Mizuta
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Yo
authors.initials: Y
authors.lastname: Kishimoto
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Tateya
authors.affiliation: Translational Research Informatics Center, The Foundation for Biomedical Research and Innovation, Kobe, Japan.|Department of Otolaryngology-Head and Neck Surgery, Medical Research Institute, Kitano Hospital, Osaka, Japan.
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Kanemaru
authors.affiliation: Department of Bioartificial Organs, Institute for Frontier Medical Science, Kyoto University, Kyoto, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Stem Cell Biology and Histology, Tohoku University, Graduate School of Medicine, Sendai, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Dezawa
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Juichi
authors.initials: J
authors.lastname: Ito
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002921:Cicatrix / Q000628:therapy*; D003094:Collagen; D003131:Combined Modality Therapy; D004285:Dogs; D045164:Mesenchymal Stem Cell Transplantation; D011446:Prospective Studies; D012038:Regeneration; D017154:Stromal Cells / Q000637:transplantation*; D014827:Vocal Cords / Q000473:pathology / Q000502:physiology*
chemical_list: C059821:atelocollagen; D003094:Collagen
keywords: Vocal fold scar; adipose tissue; bone marrow; extracellular matrix; mesenchymal stromal cells; wound healing