title: Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats.
abstract: The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, People's Republic of China, China .
authors.forename: Xufeng
authors.initials: X
authors.lastname: Han
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, People's Republic of China, China .
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, People's Republic of China, China .
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, People's Republic of China, China .
authors.forename: Bibo
authors.initials: B
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, People's Republic of China, China .
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Life Science, Yulin University, People's Republic of China, China .
authors.forename: Hailong
authors.initials: H
authors.lastname: Yan
authors.affiliation: College of Life Science, Yulin University, People's Republic of China, China .
authors.forename: Lei
authors.initials: L
authors.lastname: Qu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, People's Republic of China, China .
authors.forename: Yulin
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D004386:Duodenum / Q000382:microbiology*; D018274:Electroporation; D005453:Fluorescence; D006041:Goats / Q000382:microbiology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000502:physiology*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012417:Rumen / Q000382:microbiology*
chemical_list: D049452:Green Fluorescent Proteins
keywords: Lactobacillus plantarum; gastro-intestinal tract; goat; green fluorescent protein