title: Formaldehyde-induced paxillin-tyrosine phosphorylation and paxillin and P53 downexpression in Hela cells.
abstract: Formaldehyde (FA) is an environmental pollutant and an endogenous product believed to be involved in tumorigenesis. However, the underlying mechanism of observed FA effects has not been clearly defined. Paxillin is a focal adhesion protein that may play an important role in several signaling pathways. Many paxillin-interacting proteins are involved in the regulation of actin cytoskeleton organization, which is necessary for cell motility events associated with diverse biological responses, such as embryonic development, wound repair and tumor metastasis. P53 is important in multicellular organisms, where it regulates the cell cycle and thus functions as a tumor suppressor that is involved in preventing cancer. In this study, we investigated the effects of FA on paxillin-tyrosine phosphorylation and P53 expression in Hela cells by Western blot and immunofluorescence. Western blot analysis revealed that nonlethal concentrations of FA (0.5, 1.0 and 2.0 mM, with the exposure time for 0.5, 1.0 and 2.0 h, respectively) had downregulated paxillin and wild-type p53 genes expression while upregulated paxillin-tyrosine phosphorylation significantly. At the same time, phosphotyrosine at the focal adhesion sites detected by immunofluorescence assay obviously increased in Hela cells incubated with 2.0 mM FA for 2 h. The results suggested that paxillin and p53 genes expression may be involved in FA-related adverse effects and the mechanism may be involved in paxillin-tyrosine phosphorylation.
authors.affiliation: a Section of Environmental Biomedicine, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University , Wuhan , China .
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: b Key Laboratory of Ecological Safety Monitoring and Evaluation, College of Life Sciences, Hunan Normal University , Changsha , China , and.
authors.forename: Chenxi
authors.initials: C
authors.lastname: Wei
authors.affiliation: c College of Basic Medical Sciences, Hubei University of Science and Technology , Xianning , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: c College of Basic Medical Sciences, Hubei University of Science and Technology , Xianning , China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ma
authors.affiliation: a Section of Environmental Biomedicine, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University , Wuhan , China .
authors.forename: Shumao
authors.initials: S
authors.lastname: Ding
authors.affiliation: a Section of Environmental Biomedicine, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University , Wuhan , China .
authors.forename: Junlin
authors.initials: J
authors.lastname: Yuan
authors.affiliation: c College of Basic Medical Sciences, Hubei University of Science and Technology , Xianning , China.
authors.forename: Dingwen
authors.initials: D
authors.lastname: Shen
authors.affiliation: a Section of Environmental Biomedicine, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University , Wuhan , China .
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
mesh_terms: D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004785:Environmental Pollutants / Q000633:toxicity*; D005557:Formaldehyde / Q000633:toxicity*; D006367:HeLa Cells; D006801:Humans; D051419:Paxillin / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D004785:Environmental Pollutants; C496061:PXN protein, human; D051419:Paxillin; D016159:Tumor Suppressor Protein p53; D005557:Formaldehyde; D014443:Tyrosine
keywords: Formaldehyde; Hela cell; P53; Western blot; immunofluorescence; paxillin; phosphotyrosine