title: Rutin Increases Muscle Mitochondrial Biogenesis with AMPK Activation in High-Fat Diet-Induced Obese Rats.
abstract: Decreased mitochondrial number and dysfunction in skeletal muscle are associated with obesity and the progression of obesity-associated metabolic disorders. The specific aim of the current study was to investigate the effects of rutin on mitochondrial biogenesis in skeletal muscle of high-fat diet-induced obese rats. Supplementation with rutin reduced body weight and adipose tissue mass, despite equivalent energy intake (p < 0.05). Rutin significantly increased mitochondrial size and mitochondrial DNA (mtDNA) content as well as gene expression related to mitochondrial biogenesis, such as peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), nuclear respiratory factor-1 (NRF-1), transcription factor A (Tfam), and nicotinamide adenine dinucleotide (NAD)-dependent deacetylase, sirtulin1 (SIRT1) in skeletal muscle (p < 0.05). Moreover, rutin consumption increased muscle adenosine monophosphate-activated protein kinase (AMPK) activity by 40% (p < 0.05). Taken together, these results suggested at least partial involvement of muscle mitochondria and AMPK activation in the rutin-mediated beneficial effect on obesity.
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea. sjseo27@naver.com.
authors.forename: Sangjin
authors.initials: S
authors.lastname: Seo
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea. troph@hanmail.net.
authors.forename: Mak-Soon
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea. eugenics77@hotmail.com.
authors.forename: Eugene
authors.initials: E
authors.lastname: Chang
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea. yjin19@hotmail.com.
authors.forename: Yoonjin
authors.initials: Y
authors.lastname: Shin
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea. ohsjmay@naver.com.
authors.forename: Soojung
authors.initials: S
authors.lastname: Oh
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul 136-703, Korea. k610in@korea.ac.kr.
authors.forename: In-Hwan
authors.initials: IH
authors.lastname: Kim
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea. yhmoon@ewha.ac.kr.
authors.forename: Yangha
authors.initials: Y
authors.lastname: Kim
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D050154:Adiposity / Q000187:drug effects; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D004272:DNA, Mitochondrial / Q000378:metabolism; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D004789:Enzyme Activation; D008297:Male; D008931:Mitochondria, Muscle / Q000187:drug effects* / Q000201:enzymology; D053142:Mitochondrial Size / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000201:enzymology; D051266:Nuclear Respiratory Factor 1 / Q000235:genetics / Q000378:metabolism; D009765:Obesity / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology; D001678:Organelle Biogenesis; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D017207:Rats, Sprague-Dawley; D012431:Rutin / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015431:Weight Loss / Q000187:drug effects
chemical_list: D019440:Anti-Obesity Agents; D004272:DNA, Mitochondrial; D051266:Nuclear Respiratory Factor 1; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C501429:Ppargc1a protein, rat; C489030:Tfam protein, rat; D014157:Transcription Factors; D012431:Rutin; D055372:AMP-Activated Protein Kinases; C488368:Sirt1 protein, rat; D056564:Sirtuin 1
keywords: AMPK activity; mitochondria; obesity; rutin; skeletal muscle