title: Contribution of dietary advanced glycation end products (AGE) to circulating AGE: role of dietary fat.
abstract: The purpose of this pilot study was to determine whether macronutrient content (low-fat v. high-fat diet) influences an indicator of advanced glycation end products (AGE), N(ε) carboxymethyl-lysine (CML), in the context of a 1-d, high-AGE diet. The effect of the diets on inflammatory markers was also assessed. A total of nineteen overweight and obese adults (nine men and ten women) without known disease were recruited to participate in a crossover challenge of a high-fat, high-AGE (HFHA) and low-fat, high-AGE (LFHA) diet. In each phase patients had fasting blood drawn, followed by consumption of a high-fat or low-fat breakfast test meal, then three postprandial blood draws at 1, 2 and 3 h after consuming the test meal. After consuming high-AGE meals for the remainder of the day, participants returned the next day for a follow-up analysis. A different pattern in the 3-h post-meal CML and soluble receptor for AGE response to the two diets was observed (P=0·01 and 0·05, respectively). No change in serum CML was observed following consumption of a LFHA breakfast (535 (25th-75th percentile 451-790) to 495 (25th-75th percentile 391-682) ng/ml; P=0·36), whereas a rise in CML occurred after the HFHA breakfast (463 (25th-75th percentile 428-664) to 578 (25th-75th percentile 474-865) ng/ml; P=0·05). High sensitivity C-reactive protein and high molecular weight adiponectin were not affected by either diet. These findings suggest that dietary CML may not be as important in influencing serum CML as other dietary factors. In addition, acute exposure to dietary CML may not influence inflammation in adults without diabetes or kidney disease. This is contrary to previous findings.
authors.affiliation: 1Department of Clinical Nutrition,University of Texas Southwestern Medical Center,Dallas,TX 75390,USA.
authors.forename: Kathleen E
authors.initials: KE
authors.lastname: Davis
authors.affiliation: 2Department of Nutrition and Food Sciences,Texas Woman's University,Denton,TX 76204,USA.
authors.forename: Chandan
authors.initials: C
authors.lastname: Prasad
authors.affiliation: 2Department of Nutrition and Food Sciences,Texas Woman's University,Denton,TX 76204,USA.
authors.forename: Parakat
authors.initials: P
authors.lastname: Vijayagopal
authors.affiliation: 2Department of Nutrition and Food Sciences,Texas Woman's University,Denton,TX 76204,USA.
authors.forename: Shanil
authors.initials: S
authors.lastname: Juma
authors.affiliation: 3Department of Clinical Sciences,University of Texas Southwestern Medical Center,Dallas,TX 75390,USA.
authors.forename: Beverley
authors.initials: B
authors.lastname: Adams-Huet
authors.affiliation: 2Department of Nutrition and Food Sciences,Texas Woman's University,Denton,TX 76204,USA.
authors.forename: Victorine
authors.initials: V
authors.lastname: Imrhan
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D015992:Body Mass Index; D062408:Breakfast; D018592:Cross-Over Studies; D066273:Diet, Western / Q000009:adverse effects*; D004041:Dietary Fats / Q000009:adverse effects* / Q000378:metabolism; D005260:Female; D017127:Glycation End Products, Advanced / Q000009:adverse effects* / Q000097:blood / Q000378:metabolism; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood; D015416:Maillard Reaction; D008297:Male; D008875:Middle Aged; D050177:Overweight / Q000097:blood* / Q000378:metabolism; D010865:Pilot Projects; D019518:Postprandial Period; D000067759:Receptor for Advanced Glycation End Products / Q000097:blood* / Q000378:metabolism; D055815:Young Adult
chemical_list: D015415:Biomarkers; D004041:Dietary Fats; D017127:Glycation End Products, Advanced; D008239:Lysine; D000067759:Receptor for Advanced Glycation End Products; C048496:N(6)-carboxymethyllysine; C000593871:AGER protein, human
keywords: AGE advanced glycation end products; Advanced glycation end products; CML Nε-carboxymethyl-lysine; CRP C-reactive protein; Glycotoxins; HFHA high-fat; High-fat diet; Inflammation; LFHA low-fat; Low-fat diet; Maillard reaction products; Obesity; high-AGE; sRAGE soluble receptor for AGE