title: Optimization of conditions for probiotic curd formulation by Enterococcus faecium MTCC 5695 with probiotic properties using response surface methodology.
abstract: Enterococcus faecium MTCC 5695 possessing potential probiotic properties as well as enterocin producing ability was used as starter culture. Effect of time (12-24 h) and inoculum level (3-7 % v/v) on cell growth, bacteriocin production, antioxidant property, titrable acidity and pH of curd was studied by response surface methodology (RSM). The optimized conditions were 26.48 h and 2.17%v/v inoculum and the second order model validated. Co cultivation studies revealed that the formulated product had the ability to prevent growth of foodborne pathogens that affect keeping quality of the product during storage. The results indicated that application of E. faecium MTCC 5695 along with usage of optimized conditions attributed to the formation of highly consistent well set curd with bioactive and bioprotective properties. Formulated curd with potential probiotic attributes can be used as therapeutic agent for the treatment of foodborne diseases like Traveler's diarrhea and gastroenteritis which thereby help in improvement of bowel health.
authors.affiliation: Department of Food Microbiology, CSIR-Central Food Technological Research Institute, Mysore, 570 020 India.
authors.forename: Vrinda
authors.initials: V
authors.lastname: Ramakrishnan
authors.affiliation: Department of Food Microbiology, CSIR-Central Food Technological Research Institute, Mysore, 570 020 India.
authors.forename: Louella Concepta
authors.initials: LC
authors.lastname: Goveas
authors.affiliation: Sensory Science Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020 India.
authors.forename: Maya
authors.initials: M
authors.lastname: Prakash
authors.affiliation: Department of Food Microbiology, CSIR-Central Food Technological Research Institute, Mysore, 570 020 India.
authors.forename: Prakash M
authors.initials: PM
authors.lastname: Halami
authors.affiliation: Department of Meat, Fish and Poultry Technology, CSIR-Central Food Technological Research Institute, Mysore, 570 020 India.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Narayan
keywords: Co cultivation; Enterococcus faecium; Food borne diseases; Optimization; Probiotic curd