title: Signal transducer and activator of transcription 5B (STAT5B) modulates adipocyte differentiation via MOF.
abstract: The role and mechanism of signal transducer and activator of transcription 5B (STAT5B) in adipogenesis remain unclear. In this study, our data showed that Males absent on the first (MOF) protein expression was increased during 3 T3-L1 preadipocytes differentiation accompanied with STAT5B expression increasing. Over-expression STAT5B enhanced MOF promoter trans-activation in HeLa cells. Mutagenesis assay and ChIP analysis exhibited that STAT5B was able to bind MOF promoter. Knocking-down STAT5B in 3 T3-L1 preadipocytes led to decreased expression of MOF, but resulted in increased expression of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα) and fatty acid-binding protein 4 (Fabp4), which were important factors or enzymes for adipogenesis. We also found that knocking-down MOF in 3 T3-L1 preadipocytes resulted in increased expression of PPARγ, C/EBPα and Fabp4, which was in the same trend as STAT5B knocking-down. Over-expression MOF resulted in reduced promoter trans-activation activity of C/EBPα. These results suggest that STAT5B and MOF work as negative regulators in adipogenesis, and STAT5B modulates preadipocytes differentiation partially by regulating MOF expression.
authors.affiliation: The Department of Cell Biology, Shandong University School of Medicine, Jinan 250012, China. Electronic address: gaopengts@163.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.affiliation: The Department of Cell Biology, Shandong University School of Medicine, Jinan 250012, China; Center for Reproductive Medicine, National Research Center for Assisted reproductive Technology and Reproductive Genetics, The Key Laboratory for Reproductive Endocrinology of Ministry of Education, Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan 250021, China. Electronic address: yc.zhang@hotmail.com.
authors.forename: Yuchao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Qingdao Municipal Hospital, Qingdao 266071, China. Electronic address: sduliuyuantao@163.com.
authors.forename: Yuantao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: The Department of Cell Biology, Shandong University School of Medicine, Jinan 250012, China. Electronic address: chenjc1988@126.com.
authors.forename: Jicui
authors.initials: J
authors.lastname: Chen
authors.affiliation: The Department of Cell Biology, Shandong University School of Medicine, Jinan 250012, China. Electronic address: zongchen1203@163.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Zong
authors.affiliation: The Department of Cell Biology, Shandong University School of Medicine, Jinan 250012, China. Electronic address: yc-1127@163.com.
authors.forename: Cong
authors.initials: C
authors.lastname: Yu
authors.affiliation: The Department of Cell Biology, Shandong University School of Medicine, Jinan 250012, China. Electronic address: csup123@yeah.net.
authors.forename: Shang
authors.initials: S
authors.lastname: Cui
authors.affiliation: The Department of Cell Biology, Shandong University School of Medicine, Jinan 250012, China. Electronic address: weinana0398@163.com.
authors.forename: Weina
authors.initials: W
authors.lastname: Gao
authors.affiliation: The Department of Cell Biology, Shandong University School of Medicine, Jinan 250012, China. Electronic address: 18364135491@163.com.
authors.forename: Dandan
authors.initials: D
authors.lastname: Qin
authors.affiliation: Department of Nephrology, the Second Hospital of Shandong University, Jinan 250033, China. Electronic address: sunwenchuan111@163.com.
authors.forename: Wenchuan
authors.initials: W
authors.lastname: Sun
authors.affiliation: The Department of Cell Biology, Shandong University School of Medicine, Jinan 250012, China. Electronic address: lixia1666@sdu.edu.cn.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: The Department of Cell Biology, Shandong University School of Medicine, Jinan 250012, China; Key Laboratory of Protein Sciences for Chronic Degenerative Diseases in Universities of Shandong (Shandong University), Jinan 250012, China. Electronic address: xdongw@sdu.edu.cn.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Wang
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000502:physiology*; D050156:Adipogenesis; D000818:Animals; D001483:Base Sequence; D059305:Diet, High-Fat / Q000009:adverse effects; D006367:HeLa Cells; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D050152:Intra-Abdominal Fat / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D050799:STAT5 Transcription Factor / Q000502:physiology*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D050799:STAT5 Transcription Factor; C494096:Stat5b protein, mouse; D051548:Histone Acetyltransferases; C000622683:Kat8 protein, mouse
keywords: Adipogenesis; C/EBPα; MOF; Obesity; STAT5B