title: H2-driven biotransformation of n-octane to 1-octanol by a recombinant Pseudomonas putida strain co-synthesizing an O2-tolerant hydrogenase and a P450 monooxygenase.
abstract: An in vivo biotransformation system is presented that affords the hydroxylation of n-octane to 1-octanol on the basis of NADH-dependent CYP153A monooxygenase and NAD(+)-reducing hydrogenase heterologously synthesized in a bacterial host. The hydrogenase sustains H2-driven NADH cofactor regeneration even in the presence of O2, the co-substrate of monooxygenase.
authors.affiliation: Department of Chemistry, Technische Universität Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany. lars.lauterbach@tu-berlin.de oliver.lenz@tu-berlin.de and Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, South Parks Rd, Oxford, OX1 3QR, UK.
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Lonsdale
authors.affiliation: Department of Chemistry, Technische Universität Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany. lars.lauterbach@tu-berlin.de oliver.lenz@tu-berlin.de.
authors.forename: Lars
authors.initials: L
authors.lastname: Lauterbach
authors.affiliation: Institute of Technical Biochemistry, Universität Stuttgart, Allmandring 31, 70569, Stuttgart, Germany.
authors.forename: Sumire
authors.initials: S
authors.lastname: Honda Malca
authors.affiliation: Institute of Technical Biochemistry, Universität Stuttgart, Allmandring 31, 70569, Stuttgart, Germany.
authors.forename: Bettina M
authors.initials: BM
authors.lastname: Nestl
authors.affiliation: Institute of Technical Biochemistry, Universität Stuttgart, Allmandring 31, 70569, Stuttgart, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Hauer
authors.affiliation: Department of Chemistry, Technische Universität Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany. lars.lauterbach@tu-berlin.de oliver.lenz@tu-berlin.de.
authors.forename: Oliver
authors.initials: O
authors.lastname: Lenz
mesh_terms: D020003:1-Octanol / Q000737:chemistry / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006859:Hydrogen / Q000737:chemistry / Q000378:metabolism*; D006864:Hydrogenase / Q000378:metabolism*; D009795:Octanes / Q000737:chemistry / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D016958:Pseudomonas putida / Q000737:chemistry / Q000378:metabolism
chemical_list: D009795:Octanes; D006859:Hydrogen; D003577:Cytochrome P-450 Enzyme System; D006864:Hydrogenase; D020003:1-Octanol; D010100:Oxygen; C026728:octane