title: β-elemene inhibits monocyte-endothelial cells interactions via reactive oxygen species/MAPK/NF-κB signaling pathway in vitro.
abstract: The recruitment of monocytes to the active endothelial cells is an early step in the formation of atherosclerotic lesions; therefore, the inhibition of monocyte-endothelial cells interactions may serve as a potential therapeutic strategy for atherosclerosis. Recent studies suggest that β-elemene can protect against atherosclerosis in vivo and vitro; however, the mechanism underlying the anti-atherosclerotic effect by β-elemene is not clear yet. In this study, we aimed to investigate the effects of β-elemene on the monocyte-endothelial cells interactions in the initiation of atherosclerosis in vitro. Our results showed that β-elemene protects human umbilical vein endothelial cells (HUVECs) from hydrogen peroxide-induced endothelial cells injury in vitro. Besides, this molecule inhibits monocyte adhesion and transendothelial migration across inflamed endothelium through the suppression of the nuclear factor-kappa B-dependent expression of cell adhesion molecules. Further, β-elemene decreases generation of reactive oxygen species (ROS) and prevents the activation of mitogen-activated protein kinase (MAPK) signaling pathway in HUVECs. In conclusion, this study would provide a new pharmacological evidence of the significance of β-elemene as a future drug for prevention and treatment of atherosclerosis.
authors.affiliation: National Center for Drug Screening & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Jiangsu Province 210009, PR China; Cancer Hospital Affiliated to Xinjiang Medical University, Xinjiang, Urumqi 830011, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Liu
authors.affiliation: National Center for Drug Screening & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Jiangsu Province 210009, PR China.
authors.forename: Lifei
authors.initials: L
authors.lastname: Mao
authors.affiliation: National Center for Drug Screening & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Jiangsu Province 210009, PR China; Département de Pharmacie, Faculté de Médecine, Université Abou Bekr Belkaid, Tlemcen, Algeria.
authors.forename: Abdelkader
authors.initials: A
authors.lastname: Daoud
authors.affiliation: National Center for Drug Screening & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Jiangsu Province 210009, PR China.
authors.forename: Waseem
authors.initials: W
authors.lastname: Hassan
authors.affiliation: National Center for Drug Screening & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Jiangsu Province 210009, PR China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Zhou
authors.affiliation: National Center for Drug Screening & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Jiangsu Province 210009, PR China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Lin
authors.affiliation: National Center for Drug Screening & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Jiangsu Province 210009, PR China; Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, 24 Tong jia xiang, Nanjing 210009, Jiang Su Province, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: National Center for Drug Screening & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Jiangsu Province 210009, PR China; Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, 24 Tong jia xiang, Nanjing 210009, Jiang Su Province, PR China. Electronic address: shangjing21cn@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Shang
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012717:Sesquiterpenes / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics
chemical_list: D016328:NF-kappa B; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D012717:Sesquiterpenes; D019010:Vascular Cell Adhesion Molecule-1; C445979:beta-elemene; D018799:Intercellular Adhesion Molecule-1; D013482:Superoxide Dismutase; D020928:Mitogen-Activated Protein Kinases
keywords: Atherosclerosis; Cell adhesion; Human umbilical vein endothelial cells; NF-κB; β-elemene