title: Identification of peptides in functional Scamorza ovine milk cheese.
abstract: Ovine bulk milk was used to produce Scamorza cheese with probiotics: either a mix of Bifidobacterium longum and Bifidobacterium lactis or Lactobacillus acidophilus as the probiotic strains. Peptides obtained from reverse phase-HPLC water-soluble extract of Scamorza cheeses were analyzed using a quadrupole time-of-flight liquid chromatography-mass spectrometry system. Identified fragments were derived from casein hydrolysis or probiotic bacterial enzymes; some of the fragments showed encrypted peptide sequences that shared structural homology with previously described bioactive peptides in ovine milk and dairy products. Bifidobacterium longum and B. lactis showed greater proteolytic potential both in terms of level of pH 4.6 water-soluble nitrogen extract and ability to generate peptides with potential biofunctionality. Fragments deriving from microbial enzymes may be regarded as tracing fragments useful for monitoring probiotic activity in functional Scamorza cheese.
authors.affiliation: Department of the Sciences of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli, 25, 71100 Foggia, Italy. Electronic address: m.albenzio@unifg.it.
authors.forename: M
authors.initials: M
authors.lastname: Albenzio
authors.affiliation: Department of the Sciences of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli, 25, 71100 Foggia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Santillo
authors.affiliation: Department of the Sciences of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli, 25, 71100 Foggia, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Marino
authors.affiliation: Department of the Sciences of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli, 25, 71100 Foggia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Della Malva
authors.affiliation: Department of the Sciences of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli, 25, 71100 Foggia, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Caroprese
authors.affiliation: Department of the Sciences of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli, 25, 71100 Foggia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Sevi
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000201:enzymology / Q000378:metabolism; D002364:Caseins / Q000378:metabolism; D002611:Cheese / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007779:Lactobacillus acidophilus / Q000201:enzymology / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D008892:Milk / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000032:analysis*; D019936:Probiotics; D012756:Sheep; D034561:Sheep, Domestic
chemical_list: D002364:Caseins; D010455:Peptides; D010447:Peptide Hydrolases
keywords: ovine cheese; pasta filata; peptide; probiotic