title: Kinetics of Zinc and Cadmium Exchanges between Metallothionein and Carbonic Anhydrase.
abstract: The flexible coordination stoichiometry of a relatively high number of metal ions is a property unique to the metallothionein (MT) family of proteins. Mammalian MTs, for example, accommodate up to seven divalent metal ions in tetrahedral coordination geometries, using its complement of 20 cysteine ligands. The lability of the metals from these metalloclusters has been used to support the proposal of MTs acting as metal chaperones, by donating to other metal-binding proteins. The metal exchange kinetics between human MT1A and carbonic anhydrase (CA) were examined using time-dependent electrospray ionization mass spectrometry (ESI-MS). The time dependence of three different reaction conditions were studied: (i) zinc donation from partially metalated zinc-MT to apoCA; (ii) metal exchange between zinc saturated MTs and cadmium saturated CA (Cd-CA); and (iii) metal exchange between partially metalated zinc-MTs and Cd-CA. The results show that zinc donation from Zn-MTs to apo-zinc-dependent enzymes is dependent on the metal loading of the Znn-MT (where n = 1-7) and that this is a direct consequence of the increasing metal affinity for smaller values of n. Partially metalated MTs are also shown to extract cadmium from Cd-CA with significantly faster rates than metal saturated MTs and that even under zinc limiting conditions, mammalian Cd-CA would not coexist with MT. On the basis of these and previously published results, we suggest that protein-protein interactions between MT and CA facilitate metal transfers through favorable electrostatic interactions and hypothesize that the metal could be transferred between the MT and the enzyme active site using nearby metal-binding functionalities along the transfer pathway.
authors.affiliation: Department of Chemistry, The University of Western Ontario , London, Ontario Canada , N6A 5B7.
authors.forename: Tyler B J
authors.initials: TB
authors.lastname: Pinter
authors.affiliation: Department of Chemistry, The University of Western Ontario , London, Ontario Canada , N6A 5B7.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Stillman
mesh_terms: D000818:Animals; D001665:Binding Sites; D002104:Cadmium / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D006801:Humans; D007700:Kinetics; D008668:Metallothionein / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D021241:Spectrometry, Mass, Electrospray Ionization; D015032:Zinc / Q000378:metabolism*
chemical_list: C530099:MT1A protein, human; D002104:Cadmium; D008668:Metallothionein; D002256:Carbonic Anhydrases; D015032:Zinc