title: [MicroRNA-133a antagonizes phenylephrine-induced hypertrophy of neonatal rat cardiomyocytes in vitro].
abstract: OBJECTIVE
  To investigate the mechanism of miR-133a in reversing neonatal rat cardiomyocyte hypertrophy induced by phenylephrine.
  
  
  METHODS
  A miR-133a precursor cDNA was used to construct an adenovirus vector, which was transfected into 293 cells to harvest miR-133a-containing virus. Neonatal rat cardiac myocytes treated by phenylephrine were exposed to miR-133a adenovirus, and the changes in cell area was measured; the expression levels of miR-133a and Acta1, Actc1, Actb, Myh6, Myh7, and BNP mRNAs were detected by quantitative RT-PCR.
  
  
  RESULTS
  Phenylephrine treatment increased the area of cardiomyocytes by more than 3 folds and significantly enhanced the expression levels of Acta1, Actc1, Actb, Myh6, Myh7 and BNP mRNAs. All these changes were obviously reverse by miR-133a treatment.
  
  
  CONCLUSIONS
  miR-133a is an important regulator of phenylephrine-induced cardiomyocyte hypertrophy and negatively regulates this process.
authors.affiliation: Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Affiliated Hospital of Hainan Medical College, Haikou 570102, China.E-mail: liqi1970@hotmail.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Xiangsheng
authors.initials: X
authors.lastname: Yang
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhou
authors.forename: Lu
authors.initials: L
authors.lastname: Xiao
authors.forename: Xi
authors.initials: X
authors.lastname: Lin
authors.forename: Fengbo
authors.initials: F
authors.lastname: Zhang
authors.forename: Lingli
authors.initials: L
authors.lastname: Li
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Yu
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000256:Adenoviridae; D000818:Animals; D002478:Cells, Cultured; D005822:Genetic Vectors; D006984:Hypertrophy; D035683:MicroRNAs / Q000235:genetics*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000473:pathology*; D010656:Phenylephrine / Q000009:adverse effects*; D012333:RNA, Messenger; D051381:Rats; D014162:Transfection
chemical_list: C549446:MIRN133 microRNA, rat; D035683:MicroRNAs; D012333:RNA, Messenger; D010656:Phenylephrine