title: [Determination of Five Active Components in Morinda officinalis from Different Habitats by HPLC].
abstract: OBJECTIVE
  To establish a sensitive and specific high performance liquid chromatography (HPLC) method for simultaneous determination of alizarin anthraquinones (1-methoxy-2-hydroxy anthraquinone,1,2-dimethoxy-3-hydroxy anthraquinone, rubiadin-1- methylether, 1,3-dihydroxy-2-methoxy anthraquinone, rubiadin) in Morinda officinalis from different habitats.
  
  
  METHODS
  The analysis was carried out on an Ecosil C18 column (250 mm x 4.6 mm, 5 µm) and detected with an UV detector at the wavelength of 277 nm. Gradient elution was carried out with acetonitrile-0.2% phosphoric acid at the flow rate of 0. 8 mL/min. The column temperature was set at 30 °C.
  
  
  RESULTS
  The calibration curves were linear in the range of 0.2856-34.27 g/mL for 1-methoxy-2-hydroxy anthraquinone (r = 0.9999), 0.3268-39.22 g/mL for 1,2-dimethoxy-3-hydroxy anthraquinone (r = 0.9999), 0.3450-41.40 µg/mL for rubiadin-1- methylether (r = 0.9999), 0.1248-14. 98 µg/mL for 1,3-dihydroxy-2-methoxy anthraquinone (r = 0.9999) and 0.0508-6.096 µg/ mL for rubiadin (r = 0.9994), respectively. The average recoveries of the five components were 99.4%, 100.2%, 101.4%, 97.2%, 103.2%, respectively. The content of active components of samples from 10 different hatitats were in the range of 0.0025-0.0722 mg/ g, 0.0016-0.0658 mg/g, 0.0022-0.0684 mg/g, 0.0182-0.3965 mg/g and 0. 0014-0.0179 mg/g.
  
  
  CONCLUSIONS
  The established method is accurate, reliable, and can be used for the simultaneous determination of the five components in Morinda officinalis, which provides a scientific basis for the quality evaluation of Morinda officinalis.
authors.forename: Ji
authors.initials: J
authors.lastname: Shi
authors.forename: Zi-han
authors.initials: ZH
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Tian-zhu
authors.initials: TZ
authors.lastname: Jia
mesh_terms: D000880:Anthraquinones; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D017753:Ecosystem; D032066:Morinda / Q000737:chemistry*
chemical_list: D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; C042834:9,10-anthraquinone; C010078:alizarin; C079172:rubiadin 1-methyl ether; C072500:rubiadin