title: [Overexpression of STOML-2 inhibits apoptosis of human cervical squamous carcinoma Siha cells in vitro].
abstract: OBJECTIVE
  To investigate the mechanism underlying the inhibitory effect of STOML-2 overexpression on apoptosis of human cervical squamous carcinoma Siha cells.
  
  
  METHODS
  Siha cells were transfected with an adenoviral vector carrying STOML-2, and 72 h later STOML-2 expression and the proliferation of the cells were detected by Western blotting and MTT assay. The transfected cells were treated with IC50 Cisplatin for 24 h, and the morphological changes of cells were observed using fluorescence, and the cell apoptosis was analyzed using flow cytomerty; the expression levels of proteins related with mitochondrial apoptosis pathway, including caspase-3, cleaved caspase-3, Bcl-2, Bax and cytochrome C (Cyt C), were detected by Western blotting.
  
  
  RESULTS
  Western blotting showed a significantly increased STOML-2 expression in the transfected cells. Overexpression of STOML-2 obviously promoted the proliferation of Siha cells. The STOML-2-overexpressing cells exhibited an obvious resistance to IC50 Cisplatin-induced apoptosis as shown by both fluorescence microscopy and flow cytometry and presented with decreased expressions of cleaved caspase-3, Bax, and cytosol Cyt C and increased expressions of caspase-3, Bcl-2, and mitochondrial Cyt C.
  
  
  CONCLUSIONS
  Overexpression of STOML-2 can enhance the proliferation of Siha cells by inhibiting cell apoptosis possibly through the mitochondrial apoptosis pathway.
authors.affiliation: Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: 464033541@qq.com.
authors.forename: Guolin
authors.initials: G
authors.lastname: Hu
authors.forename: Guangyu
authors.initials: G
authors.lastname: Yao
authors.forename: Huan
authors.initials: H
authors.lastname: Deng
authors.forename: Kelei
authors.initials: K
authors.lastname: Zhao
authors.forename: Feifei
authors.initials: F
authors.lastname: Xu
authors.forename: Weijiang
authors.initials: W
authors.lastname: Liang
authors.forename: Guobing
authors.initials: G
authors.lastname: Liu
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D001798:Blood Proteins / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology; D045304:Cytochromes c / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008928:Mitochondria / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; C494826:BAX protein, human; D001798:Blood Proteins; D008565:Membrane Proteins; C406287:STOML2 protein, human; D051028:bcl-2-Associated X Protein; D045304:Cytochromes c; C505407:CASP3 protein, human; D053148:Caspase 3; D002945:Cisplatin