title: Effects of hydrogen sulfide on myocardial fibrosis in diabetic rats: Changes in matrix metalloproteinases parameters.
abstract: In order to explore the effects of hydrogen sulfide (H2S) on myocardial fibrosis in diabetic rats and its underlying mechanisms, intraperitoneal injections of streptozotocin were used to establish the diabetes models and sodium hydrosulfide (NaHS) was used as an exogenous donor of H2S. Eight weeks later, Van Gieson staining was used to observe pathological changes, and basic hydrolysis methods were adopted to measure hydroxyproline content, while Western Blot was used to determine the expression of MMP2, MMP7, MMP11, MMP13, MMP16, TIMP1 and TGFβ1.The results showed that significant myocardial fibrosis, decreased TIMP1 and MMP2 expression and increased MMP7, MMP11, MMP13, MMP16 expressions occurred in diabetic group, but all these changes were significantly reversed in diabetic rats after NaHS treatment. This suggests that H2S could attenuate cardiac fibrosis induced by diabetes and its mechanisms may be related to its modulation of MMPs/TIMPs expression and regulation of TGFβ1.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of University of South China, Hengyang, 421001, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xiao
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of University of South China, Hengyang, 421001, China.
authors.forename: Ou
authors.initials: O
authors.lastname: Zeng
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of University of South China, Hengyang, 421001, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of University of South China, Hengyang, 421001, China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of University of South China, Hengyang, 421001, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of University of South China, Hengyang, 421001, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D058065:Diabetic Cardiomyopathies / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D005355:Fibrosis; D006321:Heart / Q000187:drug effects*; D006862:Hydrogen Sulfide / Q000627:therapeutic use*; D006909:Hydroxyproline / Q000032:analysis; D008297:Male; D020782:Matrix Metalloproteinases / Q000032:analysis; D009206:Myocardium / Q000473:pathology*; D017207:Rats, Sprague-Dawley; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis; D053773:Transforming Growth Factor beta1 / Q000032:analysis
chemical_list: C576839:TIMP1 protein, rat; D019715:Tissue Inhibitor of Metalloproteinase-1; D053773:Transforming Growth Factor beta1; D020782:Matrix Metalloproteinases; D006909:Hydroxyproline; D006862:Hydrogen Sulfide
keywords: Hydrogen sulfide; diabetic myocardial fibrosis; matrix metalloproteinases; tissue inhibitors of metalloproteinases; transforming growth factor beta1