title: (1→3)-β-d-Glucan oligosaccharides monomers purification and its H2O2 induction effect study.
abstract: In order to produce highly purified (1→3)-β-d-glucan oligosaccharides ((1→3)-β-d-GOS) monomers, a hydrophilic interaction liquid chromatography (HILIC) system with X-Amide stationary phase was performed. Nine (1→3)-β-d-GOS monomers with degree of polymerization (DP) from 2 to 10 were successfully separated. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) demonstrated that these monomers were with high purity. Furthermore, a hydrogen peroxide (H2O2) online detection method was established to monitor H2O2 releases in tobacco cells. This is the first report on nine consecutive (1→3)-β-d-GOS monomers purification and its effect upon H2O2-releasing in plants. It was found that (1→3)-β-d-GOS monomers with higher DP induced stronger defense responses in plants, which will pave the way for elucidating the relationship between (1→3)-β-d-GOS and biological activities.
authors.affiliation: Natural Products and Glyco-Biotechnology Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, PR China; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China.
authors.forename: Yunbin
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Natural Products and Glyco-Biotechnology Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, PR China.
authors.forename: Mengyu
authors.initials: M
authors.lastname: Wang
authors.affiliation: Natural Products and Glyco-Biotechnology Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, PR China.
authors.forename: Wenxia
authors.initials: W
authors.lastname: Wang
authors.affiliation: Dalian Glycobio Co., Ltd., Dalian 116023, Liaoning, PR China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Tuo
authors.affiliation: Separation Materials and Separation Method Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, PR China.
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Du
authors.affiliation: Natural Products and Glyco-Biotechnology Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, PR China. Electronic address: yinheng@dicp.ac.cn.
authors.forename: Heng
authors.initials: H
authors.lastname: Yin
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006861:Hydrogen Peroxide / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D011509:Proteoglycans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D047071:beta-Glucans / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D009844:Oligosaccharides; D011509:Proteoglycans; D047071:beta-Glucans; C010770:polysaccharide-K; D006861:Hydrogen Peroxide
keywords: (1→3)-β-d-Glucan oligosaccharides; Degree of polymerization; Hydrogen peroxide; Monomer; Tobacco