title: CRISPR-Cas9: A Revolutionary Tool for Cancer Modelling.
abstract: The cancer-modelling field is now experiencing a conversion with the recent emergence of the RNA-programmable CRISPR-Cas9 system, a flexible methodology to produce essentially any desired modification in the genome. Cancer is a multistep process that involves many genetic mutations and other genome rearrangements. Despite their importance, it is difficult to recapitulate the degree of genetic complexity found in patient tumors. The CRISPR-Cas9 system for genome editing has been proven as a robust technology that makes it possible to generate cellular and animal models that recapitulate those cooperative alterations rapidly and at low cost. In this review, we will discuss the innovative applications of the CRISPR-Cas9 system to generate new models, providing a new way to interrogate the development and progression of cancers.
authors.affiliation: Viral Vector Technical Unit, Fundacion Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fernandez Almagro, 3, 28029 Madrid, Spain. truizraul27@gmail.com.
authors.forename: Raul
authors.initials: R
authors.lastname: Torres-Ruiz
authors.affiliation: Molecular Cytogenetics Group, Human Cancer Genetics Program, Centro Nacional de Investigaciones Oncológicas (CNIO), Melchor Fernandez Almagro, 3, 28029 Madrid, Spain. srodriguezp@cnio.es.
authors.forename: Sandra
authors.initials: S
authors.lastname: Rodriguez-Perales
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D063646:Carcinogenesis / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods; D006801:Humans; D008957:Models, Genetic
keywords: CRISPR-Cas9 system; cancer modelling; gene mutations; genome engineering; rearrangements