title: Microfluidic assay-based optical measurement techniques for cell analysis: A review of recent progress.
abstract: Since the early 2000s, microfluidic cell culture systems have attracted significant attention as a promising alternative to conventional cell culture methods and the importance of designing an efficient detection system to analyze cell behavior on a chip in real time is raised. For this reason, various measurement techniques for microfluidic devices have been developed with the development of microfluidic assays for high-throughput screening and mimicking of in vivo conditions. In this review, we discuss optical measurement techniques for microfluidic assays. First of all, the recent development of fluorescence- and absorbance-based optical measurement systems is described. Next, advanced optical detection systems are introduced with respect to three emphases: 1) optimization for long-term, real-time, and in situ measurements; 2) performance improvements; and 3) multimodal analysis conjugations. Moreover, we explore presents future prospects for the establishment of optical detection systems following the development of complex, multi-dimensional microfluidic cell culture assays to mimic in vivo tissue, organ, and human systems.
authors.affiliation: Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), 701-310 Daegu, Republic of Korea.
authors.forename: Jong-Ryul
authors.initials: JR
authors.lastname: Choi
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, 609-735 Busan, Republic of Korea.
authors.forename: Hyerin
authors.initials: H
authors.lastname: Song
authors.affiliation: Department of Chemical Engineering, Hongik University, 121-791 Seoul, Republic of Korea. Electronic address: jhsung22@hongik.ac.kr.
authors.forename: Jong Hwan
authors.initials: JH
authors.lastname: Sung
authors.affiliation: School of Electrical and Electronic Engineering, Yonsei University, Seoul, Korea, 120-749, Republic of Korea.
authors.forename: Donghyun
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, 609-735 Busan, Republic of Korea; Department of Opto-Mechatronics Engineering,Pusan National University, 609-735 Busan, Republic of Korea. Electronic address: k.kim@pusan.ac.kr.
authors.forename: Kyujung
authors.initials: K
authors.lastname: Kim
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002469:Cell Separation / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D055096:Optical Devices; D046888:Tissue Array Analysis / Q000295:instrumentation*
keywords: Cell analysis; In situ measurement; Microfluidic assays; Multimodal analysis; Optical measurements; Performance improvement