title: Production of single cell oil from Lipomyces starkeyi ATCC 56304 using biorefinery by-products.
abstract: Single cell oil (SCO) is a valuable noncrop-based renewable oil source. Hemicellulose derived sugars can be utilized to produce SCO using the oleaginous yeast Lipomyces starkeyi ATCC 56304. Bran by-products were tested as hemicellulose-rich feedstocks for the production of SCO. Whole and destarched corn and wheat bran hydrolysates were produced using hydrothermal and dilute sulfuric acid (0%, 0.5%, 1.0%, v/v) pretreatment along with enzymatic hydrolysis. Whole bran hydrolysates produced from hydrothermal pretreatment generated the highest average oil yields of 126.7 and 124.3 mg oil/g sugar for both wheat and corn bran, respectively. 1.0% acid pretreatment was effective for the destarched bran generating a hemicellulose hydrolysis efficiency of 94% and 84% for wheat and corn bran, respectively, resulting in the highest oil yield of 70.7 mg oil/g sugar. The results indicate pretreated corn and wheat bran hydrolysates can serve as viable feedstocks for oleaginous yeast SCO bioconversion.
authors.affiliation: IGERT in Bioprocessing, Department of Grain Science and Industry, Kansas State University, BIVAP Building, 1980 Kimball Ave., Manhattan, KS 66506, United States. Electronic address: kyle.probst@grainprocessing.com.
authors.forename: Kyle V
authors.initials: KV
authors.lastname: Probst
authors.affiliation: Bioprocessing and Renewable Energy Lab, Departments of Grain Science and Industry & Chemical Engineering, Kansas State University, BIVAP Building, 1980 Kimball Ave., Manhattan, KS 66506, United States.
authors.forename: Praveen V
authors.initials: PV
authors.lastname: Vadlani
mesh_terms: D004043:Dietary Fiber; D005285:Fermentation / Q000502:physiology*; D006868:Hydrolysis; D055160:Lipomyces / Q000378:metabolism*; D013464:Sulfuric Acids; D003313:Zea mays
chemical_list: D004043:Dietary Fiber; D013464:Sulfuric Acids; C033158:sulfuric acid
keywords: Bran; Fermentation; Oleaginous yeast; Pretreatment; Single cell oil