title: A sulfated polysaccharide of Ecklonia cava inhibits the growth of colon cancer cells by inducing apoptosis.
abstract: We investigated anticancer effects of the crude polysaccharides (CPs) isolated from Ecklonia cava enzymatic extracts using AMG, Viscozyme, Protamex, and Alcalase enzyme against a colon cancer cell line, CT26 cells. Among them, the CP of Protamex extract (PCP) contained the highest fucose and sulfated group contents and showed the highest growth inhibitory effect against CT-26 cells. In addition, PCP dose-dependently increased the formation of apoptotic body and the percentage of Sub-G1 DNA contents. Also, PCP activated caspase 9 and PARP as regulating the expressions of Bax and Bcl-2. Moreover, PPP2, a fraction purified from PCP showed the highest growth inhibitory effect against CT 26 cells with the increased fucose and sulfated group contents. The results demonstrate that the isolated SP containing plentiful fucose and sulfated group contents has the anticancer effect on colon cancer cells via regulation of Bcl-2/Bax signal pathway.
authors.affiliation: Department of Marine Bio-Food Science, Chonnam National University, Yeosu 550-749, Republic of Korea.
authors.forename: Ginnae
authors.initials: G
authors.lastname: Ahn
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: WonWon
authors.initials: W
authors.lastname: Lee
authors.affiliation: Jeju center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea.
authors.forename: Kil-Nam
authors.initials: KN
authors.lastname: Kim
authors.affiliation: Jeju center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea.
authors.forename: Ji-Hyeok
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Global Bioresources Research Center, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Heo
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Nalae
authors.initials: N
authors.lastname: Kang
authors.affiliation: Division of Food Bioscience and Korea Nokyong Research Center, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Seung-Hong
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Division of Food and Nutrition, Chonnam National University, Gwangju 550-757, Republic of Korea.
authors.forename: Chang-Bum
authors.initials: CB
authors.lastname: Ahn
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
keywords: Ecklonia cava; apoptosis; enzyme-assisted extraction; murine colon cancer cells (CT-26 cells); sulfated polysaccharides