title: Effect of photosystem I inactivation on chlorophyll a fluorescence induction in wheat leaves: Does activity of photosystem I play any role in OJIP rise?
abstract: Interpretation of the fast chlorophyll a fluorescence induction is still a subject of continuing discussion. One of the contentious issues is the influence of photosystem I (PSI) activity on the kinetics of the thermal JIP-phase of OJIP rise. To demonstrate this influence, we realized a series of measurements in wheat leaves subjected to PSI photoinactivation by the sequence of red saturation pulses (15,000 μmol photons m(-2) s(-1) for 0.3 s, every 10 s) applied in darkness. Such a treatment led to a moderate decrease of maximum quantum efficiency of PSII (by ~8%), but a strong decrease of the number of oxidizable PSI (by ~55%), which considerably limited linear electron transport and CO2 assimilation. Surprisingly, the PSI photoinactivation had low effects on OJIP kinetics of variable fluorescence. In particular, the amplitude of variable fluorescence of IP-step (ΔVIP), which has been considered to be a measure of PSI content, was not decreased, despite the low content of photooxidizable PSI. On the other hand, the slower relaxation of chlorophyll fluorescence after saturation pulse as well as the results of the double-hit method suggest that PSI inactivation treatment led to an increase of the fraction of QB-nonreducing PSII reaction centers. Our results somewhat challenge the mainstream interpretations of JIP-thermal phase, and at least suggest that the IP amplitude cannot serve to estimate reliably the PSI content or the PSI to PSII ratio. Moreover, these results recommend the use of the novel method of PSI inactivation, which might help clarify some important issues needed for the correct understanding of the OJIP fluorescence rise.
authors.affiliation: Department of Plant Physiology, Slovak Agricultural University, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic. Electronic address: marian.brestic@uniag.sk.
authors.forename: Marek
authors.initials: M
authors.lastname: Zivcak
authors.affiliation: Department of Plant Physiology, Slovak Agricultural University, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic.
authors.forename: Marian
authors.initials: M
authors.lastname: Brestic
authors.affiliation: Department of Plant Physiology, Slovak Agricultural University, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic.
authors.forename: Kristyna
authors.initials: K
authors.lastname: Kunderlikova
authors.affiliation: Department of Plant Physiology, Slovak Agricultural University, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic.
authors.forename: Katarina
authors.initials: K
authors.lastname: Olsovska
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, Russia; Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia; Department of Plant Physiology, Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, Moscow 119991, Russia. Electronic address: suleyman.allakhverdiev@gmail.com.
authors.forename: Suleyman I
authors.initials: SI
authors.lastname: Allakhverdiev
mesh_terms: D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D007700:Kinetics; D008027:Light; D010788:Photosynthesis / Q000528:radiation effects; D045331:Photosystem I Protein Complex / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014908:Triticum / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects*
chemical_list: D045331:Photosystem I Protein Complex; D002734:Chlorophyll; D000077194:Chlorophyll A
keywords: Chlorophyll fluorescence; OJIP; P700; PSI photoinhibition; Photosynthesis