title: Studies towards the stabilisation of a mushroom phytase produced by submerged cultivation.
abstract: A novel phytase from Ganoderma australe G24 was produced by submerged cultivation and recovery. Liquid and solid forms of phytase were developed; both types of product were formulated using different additives. Ganoderma australe G24 phytase was very stable in liquid form with NaCl and sodium acetate buffer. Solid form products were obtained by spray-drying using different polymers to encapsulate the phytase and the capsules obtained were analyzed by electron microscopy. Micrographs confirmed micro and nanoparticles formed with maltodextrin (300 nm to 7-8 µm) without the presence of agglomerates. The use of maltodextrin for solid formulation of G. australe G24 phytase is recommended, and resulted in good stability after the drying process and during storage (shelf life). Kinetic models of phytase inactivation in the microencapsulated powders over time were proposed for the different stabilizing additives. Inactivation rate constants, half-lives and D values (decimal reduction time) were obtained. Phytase encapsulated with maltodextrin remained stable after 90 days, with k 0.0019 day(-1) and a half-life (t1/2) of 367.91 days(-1).
authors.affiliation: Technology Sector, Chemical Engineering Department, Post Graduate Program in Food Engineering, Federal University of Paraná, P.O. Box 19011, Curitiba, Paraná, 81531-980, Brazil. spier@ufpr.br.
authors.forename: Michele Rigon
authors.identifier: http://orcid.org/0000-0002-7179-0586
authors.initials: MR
authors.lastname: Spier
authors.affiliation: Department of Food Technology and Bioprocess Engineering, Max Rubner-Institut, Haid-und-Neu-Str. 9, 76131, Karlsruhe, Germany.
authors.forename: Diana
authors.initials: D
authors.lastname: Behsnilian
authors.affiliation: Technology Sector, Chemical Engineering Department, Post Graduate Program in Food Engineering, Federal University of Paraná, P.O. Box 19011, Curitiba, Paraná, 81531-980, Brazil.
authors.forename: Acácio
authors.initials: A
authors.lastname: Zielinski
authors.affiliation: Waldstrasse, Dettenheim, Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: Konietzny
authors.affiliation: Department of Food Technology and Bioprocess Engineering, Max Rubner-Institut, Haid-und-Neu-Str. 9, 76131, Karlsruhe, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Greiner
mesh_terms: D010832:6-Phytase / Q000737:chemistry* / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism*; D032902:Ganoderma / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D010832:6-Phytase
keywords: Ganoderma australe; Phytase; Polymers; SEM-analysis; Spray-dryer; Stability