title: Preliminary evaluation of hepatoprotective potential of the polyherbal formulation.
abstract: OBJECTIVE
  The aim of this study was to investigate the antioxidant and hepatoprotective effects of the polyherbal formulation (PHF)containing Cajanus cajan (L.)Millsp., Lawsonia inermis L. Linn, Mimosa pudica L., Uraria picta (Jacq.)DC. and Operculina turpethum (L.)Silva Manso on carbon tetrachloride (CCl4)induced acute liver damage in albino rats.
  
  
  METHODS
  The groups of animals were administered with PHF at the doses 100, 200 and 400 mg/kg b.w. (per oral [p.o.])once in a day for 7 days and at day 6th and 7th the animals were administrated with Carbon tetrachloride (1.0 mL/kg b.w. 50% v/v with olive oil,; p.o.). The effect of PHF on serum glutamine pyruvate transaminase (SGPT), serum glutamine oxaloacetate transaminase, alkaline phosphatase (ALP)and total bilirubin were determined in CCl4 - induced hepatotoxicity in rats. Further, the effects of PHF on glutathione (GSH), superoxide dismutase (SOD)level and lipid peroxidation (LPO)activity were also investigated.
  
  
  RESULTS
  The results demonstrated that PHF (400 mg/kg b.w.)significantly reduces the CCl4 induced increase in level of serum SGPT, serum ALP and total bilirubin. PHF (400 mg/kg b.w.)prevents the depletion level of GSH and decrease in the activity of SOD in CCl4 -induced liver injury in rats. In addition, PHF also showed a significant decrease in the LPO levels signifying the potent antioxidant activity.
  
  
  CONCLUSIONS
  All our findings suggest that PHF could protect the liver cells from CCl4 - induced liver damages and the mechanism may be through the anti-oxidative effect of PHF.
authors.affiliation: Department of Ayurvedic Pharmacy, Ayurvedic Pharmacy Laboratory, Rajiv Gandhi South Campus, Banaras Hindu University, Barkachha, Mirzapur, Uttar Pradesh, India.
authors.forename: Ghosh
authors.initials: G
authors.lastname: Arka
authors.affiliation: Department of Ayurvedic Pharmacy, Ayurvedic Pharmacy Laboratory, Rajiv Gandhi South Campus, Banaras Hindu University, Barkachha, Mirzapur, Uttar Pradesh, India.
authors.forename: Kundu
authors.initials: K
authors.lastname: Anindita
authors.affiliation: Department of Ayurvedic Pharmacy, Ayurvedic Pharmacy Laboratory, Rajiv Gandhi South Campus, Banaras Hindu University, Barkachha, Mirzapur, Uttar Pradesh, India.
authors.forename: Seth
authors.initials: S
authors.lastname: Ankit
authors.affiliation: Department of Dravyaguna, Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh.
authors.forename: Singh Anil
authors.initials: SA
authors.lastname: Kumar
authors.affiliation: Department of Ayurvedic Pharmacy, Ayurvedic Pharmacy Laboratory, Rajiv Gandhi South Campus, Banaras Hindu University, Barkachha, Mirzapur, Uttar Pradesh, India.
authors.forename: Maurya Santosh
authors.initials: MS
authors.lastname: Kumar
keywords: Antioxidant; a polyherbal formulation; carbon tetrachloride; hepatoprotective