title: EMMPRIN regulates tumor growth and metastasis by recruiting bone marrow-derived cells through paracrine signaling of SDF-1 and VEGF.
abstract: EMMPRIN, a cell adhesion molecule highly expressed in a variety of tumors, is associated with poor prognosis in cancer patients. Mechanistically, EMMPRIN has been characterized to contribute to tumor development and progression by controlling the expression of MMPs and VEGF. In the present study, by using fluorescently labeled bone marrow-derived cells (BMDCs), we found that the down-regulation of EMMPRIN expression in cancer cells reduces tumor growth and metastasis, and is associated with the reduced recruitment of BMDCs. Further protein profiling studies suggest that EMMPRIN controls BMDC recruitment through regulating the secretion of soluble factors, notably, VEGF and SDF-1. We demonstrate that the expression and secretion of SDF-1 in tumor cells are regulated by EMMPRIN. This study reveals a novel mechanism by which EMMPRIN promotes tumor growth and metastasis by recruitment of BMDCs through controlling secretion and paracrine signaling of SDF-1 and VEGF.
authors.affiliation: Experiment Center of Biomedical Research School of Medicine, Xi'an Jiaotong University, Xi'an 710061, P. R. China.|Department of Neurosurgery, Yale University, New Haven, CT 06511, USA.
authors.forename: Yanke
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Yale University, New Haven, CT 06511, USA.|Laboratory of Cell Biology and Translational Medicine, Xi'an Medical University, Xi'an 710021, P. R. China.
authors.forename: Xingchun
authors.initials: X
authors.lastname: Gou
authors.affiliation: Department of Neurosurgery, Yale University, New Haven, CT 06511, USA.
authors.forename: Derek Kai
authors.initials: DK
authors.lastname: Kong
authors.affiliation: Experiment Center of Biomedical Research School of Medicine, Xi'an Jiaotong University, Xi'an 710061, P. R. China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, Yale University, New Haven, CT 06511, USA.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Yale University, New Haven, CT 06511, USA.
authors.forename: Zeming
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Experiment Center of Biomedical Research School of Medicine, Xi'an Jiaotong University, Xi'an 710061, P. R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Neurosurgery, Yale University, New Haven, CT 06511, USA.|Department of Biomedical Engineering, Yale University, New Haven, CT 06510, USA.
authors.forename: Jiangbing
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000818:Animals; D051926:Basigin / Q000235:genetics / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000473:pathology; D016026:Bone Marrow Transplantation; D018827:Carcinoma, Lewis Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism*; D002633:Chemotaxis; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009362:Neoplasm Metastasis; D019899:Paracrine Communication; D040901:Proteomics / Q000379:methods; D034622:RNA Interference; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D047368:Tumor Burden; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: C497886:BSG protein, human; C497887:Bsg protein, mouse; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; C515256:Cxcl12 protein, mouse; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D051926:Basigin
keywords: EMMPRIN; SDF-1; VEGF; bone marrow-derived cells; tumor growth and metastasis