title: Bioinformatics and Molecular Biological Characterization of a Hypothetical Protein SAV1226 as a Potential Drug Target for Methicillin/Vancomycin-Staphylococcus aureusInfections.
abstract: Methicillin/multiple-resistantStaphylococcus aureus(MRSA) are infectious bacteria that are resistant to common antibiotics. A previousin silicostudy in our group has identified a hypothetical protein SAV1226 as one of the potential drug targets. In this study, we reported the bioinformatics characterization, as well as cloning, expression, purification and kinetic assays of hypothetical protein SAV1226 from methicillin/vancomycin-resistantStaphylococcus aureusMu50 strain. MALDI-TOF/MS analysis revealed a low degree of structural similarity with known proteins. Kinetic assays demonstrated that hypothetical protein SAV1226 is neither a domain of an ATP dependent dihydroxyacetone kinase nor of a phosphotransferase system (PTS) dihydroxyacetone kinase, suggesting that the function of hypothetical protein SAV1226 might be misannotated on public databases such as UniProt and InterProScan 5.
authors.affiliation: The Natural Sciences Division, Mount Marty College, Yankton, SD 57078 USA.
authors.forename: Nichole
authors.initials: N
authors.lastname: Haag
authors.affiliation: Mount Marty College, Yankton, SD 57078 USA, She is now with Yankton High School.
authors.forename: Kimberly
authors.initials: K
authors.lastname: Velk
authors.affiliation: Mount Marty College, Yankton, SD 57078 USA.
authors.forename: Tyler
authors.initials: T
authors.lastname: McCune
authors.affiliation: The Natural Sciences Division, Mount Marty College, Yankton, SD 57078 USA.
authors.forename: Chun
authors.initials: C
authors.lastname: Wu
keywords: Dihydroxyacetone kinase; Methicillin-resistant Staphylococcus aureus; drug target; essential genes