title: Analysis of the Fam181 gene family during mouse development reveals distinct strain-specific expression patterns, suggesting a role in nervous system development and function.
abstract: During somitogenesis differential gene expression can be observed for so-called cyclic genes, which display expression changes with a periodicity of 120min in the mouse. In screens to identify novel cyclic genes in murine embryos, Fam181b was predicted to be an oscillating gene in the presomitic mesoderm (psm). This gene, and its closely related paralog Fam181a, belong to the thus far uncharacterized Fam181 gene family. Here we describe the expression of Fam181b and Fam181a during murine embryonic development. In addition, we confirm oscillation of Fam181b in the psm in-phase with targets of, and regulated by, Notch signaling. Fam181b expression in the psm, as well as in the lateral plate mesoderm, was found to be affected by genetic background. We show that Fam181a and b exhibit partially overlapping mRNA expression patterns, and encode for proteins containing highly-conserved motifs, which predominantly localize to the nucleus. A Fam181b loss-of-function model was generated and found to result in no obvious phenotype.
authors.affiliation: Max Planck Institute for Molecular Genetics, Developmental Genetics Department, Ihnestraße 63-73, 14195 Berlin, Germany; Free University Berlin, Dept. of Biology, Chemistry and Pharmacy, Takustrasse 3, 14195 Berlin, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Marks
authors.affiliation: Max Planck Institute for Molecular Genetics, Developmental Genetics Department, Ihnestraße 63-73, 14195 Berlin, Germany.
authors.forename: Tracie
authors.initials: T
authors.lastname: Pennimpede
authors.affiliation: Max Planck Institute for Molecular Genetics, Developmental Genetics Department, Ihnestraße 63-73, 14195 Berlin, Germany; Free University Berlin, Dept. of Biology, Chemistry and Pharmacy, Takustrasse 3, 14195 Berlin, Germany.
authors.forename: Lisette
authors.initials: L
authors.lastname: Lange
authors.affiliation: Max Planck Institute for Molecular Genetics, Developmental Genetics Department, Ihnestraße 63-73, 14195 Berlin, Germany.
authors.forename: Phillip
authors.initials: P
authors.lastname: Grote
authors.affiliation: Max Planck Institute for Molecular Genetics, Developmental Genetics Department, Ihnestraße 63-73, 14195 Berlin, Germany; Charité-University Medicine Berlin, Institute for Medical Genetics, Campus Benjamin Franklin, Hindenburgdamm 30, 12203 Berlin, Germany.
authors.forename: Bernhard G
authors.initials: BG
authors.lastname: Herrmann
authors.affiliation: Max Planck Institute for Molecular Genetics, Developmental Genetics Department, Ihnestraße 63-73, 14195 Berlin, Germany. Electronic address: wittler@molgen.mpg.de.
authors.forename: Lars
authors.initials: L
authors.lastname: Wittler
mesh_terms: D000818:Animals; D001683:Biological Clocks; D002454:Cell Differentiation; D002460:Cell Line; D047108:Embryonic Development; D053595:Embryonic Stem Cells / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D051880:Receptors, Notch / Q000378:metabolism; D015398:Signal Transduction; D019170:Somites / Q000254:growth & development*
chemical_list: C000605861:FAM181b protein, mouse; D009419:Nerve Tissue Proteins; D051880:Receptors, Notch
keywords: Background specificity; Mouse embryogenesis; Neural development; Notch signaling; Somitogenesis