title: Effects of Administration of Amlodipine and Lacidipine on Inflammation-Induced Bone Loss in the Ovariectomized Rat.
abstract: This study was performed to evaluate the possible protective effect of two calcium channel blocker's "lacidipine (LAC) and amlodipine (AML)" on bone metabolism in an experimental ovariectomized and inflammation-induced osteoporosis rat model (OVXinf). For the purpose of this study, the rats were divided into eight groups, each containing eight rats: sham-operated control (group 1, SH), sham + inflammation (group 2, SHinf), ovariectomy (group 3, OVX), ovariectomy + inflammation (group 4, OVXinf), ovariectomy + LAC 4 mg/kg (group 5, OVX + LAC), ovariectomy + inflammation + LAC 4 mg/kg (group 6, OVXinf + LAC), ovariectomy + AML 5 mg/kg (group 7, OVX + AML), ovariectomy + inflammation + AML 5 mg/kg (group 8, OVXinf + AML). The levels of osteocalcin and osteopontin decreased in OVXinf + LAC and OVXinf + AML groups. The serum levels of TNF-α, IL-1β, and IL-6 were increased significantly in the OVXinf rats compared with the SH group. Gene expression levels of the osteogenic factor runt-related transcription factor 2 (Runx2) and type I collagen 1A1 (Col1A1) significantly decreased in the OVXinf group, when compared with the control group. AML or LAC administrations increased the levels of Runx2 and Col1A1. These results suggest that amlodipine and lacidipine may be a novel therapeutic target for radical osteoporosis treatment in hypertensive patients.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey. ekarakus@atauni.edu.tr.
authors.forename: Emre
authors.initials: E
authors.lastname: Karakus
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Zekai
authors.initials: Z
authors.lastname: Halici
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Abdulmecit
authors.initials: A
authors.lastname: Albayrak
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey.
authors.forename: Yasin
authors.initials: Y
authors.lastname: Bayir
authors.affiliation: Department of Pathology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Demirci
authors.affiliation: Department of Orthopedics and Traumatology, Ataturk University Faculty of Medicine, Erzurum, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Aydin
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Ibrahim Cecen University, Agrı, Turkey.
authors.forename: Berna
authors.initials: B
authors.lastname: Ozturk-Karagoz
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Cadirci
authors.affiliation: Department of Nuclear Medicine, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Arif Kursat
authors.initials: AK
authors.lastname: Ayan
authors.affiliation: Department of Nuclear Medicine, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Sahin
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Unal
mesh_terms: D017311:Amlodipine / Q000494:pharmacology*; D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D024042:Collagen Type I / Q000096:biosynthesis; D000090602:Collagen Type I, alpha 1 Chain; D050659:Core Binding Factor Alpha 1 Subunit / Q000096:biosynthesis; D004095:Dihydropyridines / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000188:drug therapy; D007249:Inflammation / Q000473:pathology; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D015675:Osteocalcin / Q000378:metabolism; D053495:Osteopontin / Q000378:metabolism; D010024:Osteoporosis / Q000517:prevention & control*; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D002121:Calcium Channel Blockers; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D050659:Core Binding Factor Alpha 1 Subunit; D004095:Dihydropyridines; C000594089:IL1B protein, rat; D053583:Interleukin-1beta; D015850:Interleukin-6; C493717:Runx2 protein, rat; D014409:Tumor Necrosis Factor-alpha; D015675:Osteocalcin; D053495:Osteopontin; D017311:Amlodipine; C060285:lacidipine
keywords: amlodipine; bone mineral density; inflammation; lacidipine; ovariectomized rat