title: AlteredTNFAIP3mRNA expression in peripheral blood mononuclear cells from patients with rheumatoid arthritis.
abstract: The tumor necrosis factor α-induced protein-3 (TNFAIP3) gene functions in negative immunoregulation and its single-nucleotide polymorphisms (SNPs) are associated with rheumatoid arthritis (RA) disease. However, its expression level in immune cells from RA patients remains unclear. The aim of the present study was to investigate whether the expression ofTNFAIP3is changed in patients with RA. Reverse transcription-quantitative polymerase chain reaction analysis was used to determineTNFAIP3mRNA expression in peripheral blood mononuclear cells (PBMCs) from patients with RA and healthy controls.TNFAIP3expression was decreased in RA patients compared with the healthy controls. The expression level of theTNFAIP3gene negatively correlated with the RA score, anti-cyclic citrullinated peptide (CCP) antibody levels and C-reactive protein levels. Furthermore, RA patients with positive results of anti-CCP antibodies had a lower expression ofTNFAIP3than those without anti-CCP antibodies. In conclusion, the present results suggest that the insufficient expression of theTNFAIP3gene in PBMCs may correlate with the diagnosis of RA.
authors.affiliation: Clinical Laboratory, Qingdao Municipal Hospital, Qingdao, Shandong 266011, P.R. China.
authors.forename: Zhaoyan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Urology, Qingdao Municipal Hospital, Qingdao, Shandong 266011, P.R. China.
authors.forename: Zongliang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Clinical Laboratory, Qingdao Municipal Hospital, Qingdao, Shandong 266011, P.R. China.
authors.forename: Jiangshui
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Clinical Laboratory, Qingdao Municipal Hospital, Qingdao, Shandong 266011, P.R. China.
authors.forename: L I
authors.initials: LI
authors.lastname: Li
keywords: reverse transcription-quantitative polymerase chain reaction analysis; rheumatoid arthritis; tumor necrosis factor α-induced protein-3