title: Interferon-stimulated gene factor 3 complex is required for the induction of sterile α motif and HD domain-containing protein 1 expression by interferon-α in SMMC-7721 cells.
abstract: Sterile α motif and HD domain-containing protein 1 (SAMHD1) is a novel intrinsic restriction factor that inhibits the replication of certain retroviruses and DNA viruses through its deoxynucleoside triphosphate triphosphohydrolase activity. A previous study by our group showed that SAMHD1 restrained hepatitis B virus replication and interferon (IFN)‑α induced SAMHD1 expression in liver cells. However the mechanisms of SAMHD1 upregulation by IFN‑α in liver cells have remained elusive. The present study demonstrated that IFN‑α treatment increased SAMHD1 mRNA levels in SMMC‑7721 cells in a time‑dependent manner. Knockdown of STAT1 inhibited the induction of SAMHD1 expression by IFN‑α in SMMC‑7721 cells. STAT2 silencing also suppressed the induction of SAMHD1 expression by IFN‑α in SMMC‑7721 cells. Furthermore, the induction of SAMHD1 expression in SMMC‑7721 cells by IFN‑α was found to be dependent on IFN‑regulatory factor 9 (IRF9). In conclusion, these results suggested that the interferon‑stimulated gene factor 3 complex, which consists of STAT1, STAT2 and IRF9, is required for the induction of SAMHD1 expression by IFN-α in SMMC-7721 cells.
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors.forename: Mengying
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors.forename: Jinsen
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors.forename: Xiaowan
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zheng
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors.forename: Zhangming
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Pan
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors.forename: Yongji
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors.forename: Hai
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Pharmacology, School of Basic Medical Sciences and Biopharmaceutical Research Institute, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors.forename: Yuxian
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors.forename: Songcheng
authors.initials: S
authors.lastname: Ying
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors.forename: Chunsong
authors.initials: C
authors.lastname: Hu
mesh_terms: D045744:Cell Line, Tumor; D015870:Gene Expression; D006801:Humans; D050832:Interferon-Stimulated Gene Factor 3 / Q000502:physiology*; D016898:Interferon-alpha / Q000502:physiology*; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D000076106:SAM Domain and HD Domain-Containing Protein 1; D015533:Transcriptional Activation
chemical_list: D050832:Interferon-Stimulated Gene Factor 3; D016898:Interferon-alpha; D000076106:SAM Domain and HD Domain-Containing Protein 1; C484280:SAMHD1 protein, human; D020559:Monomeric GTP-Binding Proteins