title: Antimicrobial activity of Anonna mucosa (Jacq.) grown in vivo and obtained by in vitroculture.
abstract: Brazilian flora includes numerous species of medicinal importance that can be used to develop new drugs. Plant tissue culture offers strategies for conservation and use of these species allowing continuous production of plants and bioactive substances. Annona mucosa has produced substances such as acetogenins and alkaloids that exhibit antimicrobial activities. The widespread use of antibiotics has led to an increase in multidrug-resistant bacteria, which represents a serious risk of infection. In view of this problem, the aim of this work was to evaluate the antibacterial potential of extracts of A. mucosa obtained by in vitro techniques and also cultured under in vivo conditions. Segments from seedlings were inoculated onto different culture media containing the auxin picloram and the cytokinin kinetin at different concentrations. The calluses obtained were used to produce cell suspension cultures. The materials were subjected to methanol extraction and subsequent fractionation in hexane and dichloromethane. The antimicrobial activity against 20 strains of clinical relevance was evaluated by the macrodilution method at minimum inhibitory and minimum bactericidal concentrations. The extracts showed selective antimicrobial activity, inhibiting the growth of Streptococcus pyogenes and Bacillus thuringiensis at different concentrations. The plant tissue culture methods produced plant materials with antibacterial properties, as well as in vivo grown plants. The antibacterial activity of material obtained through biotechnological procedures of A. mucosa is reported here for the first time.
authors.affiliation: Departamento de Biologia Vegetal, Universidade do Estado do Rio de Janeiro, Maracanã, RJ, Brazil .
authors.forename: Thiago José de Souza
authors.initials: TJ
authors.lastname: Barboza
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil .
authors.forename: Andréa Fonseca
authors.initials: AF
authors.lastname: Ferreira
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil .
authors.forename: Ana Claudia de Paula Rosa
authors.initials: AC
authors.lastname: Ignacio
authors.affiliation: Departamento de Biologia Vegetal, Universidade do Estado do Rio de Janeiro, Maracanã, RJ, Brazil .
authors.forename: Norma
authors.initials: N
authors.lastname: Albarello
mesh_terms: D031041:Annona / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001413:Bacillus thuringiensis / Q000187:drug effects / Q000254:growth & development*; D001938:Brazil; D018929:Cell Culture Techniques; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000378:metabolism*; D013297:Streptococcus pyogenes / Q000187:drug effects / Q000254:growth & development*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts
keywords: Annonaceae; antibacterial properties; callus cultures; cell suspension cultures; medicinal plant