title: Bacillus cellulasensis sp. nov., isolated from marine sediment.
abstract: A novel bacterial strain NIO-1130(T) was isolated from sediment sample taken from Chorao Island, Goa Province, India, and subjected to a taxonomic investigation. The strain was Gram-positive, aerobic, and motile. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate within the genus Bacillus and strain NIO-1130(T) showed highest sequence similarity with Bacillus halosaccharovorans DSM 25387(T) (98.4%) and Bacillus niabensis CIP 109816(T) (98.1%), whereas other Bacillus species showed <97.0% similarity. Tree based on gyrB gene sequence revealed that strain bacillus group. The major menaquinone was MK-7 and the predominant cellular fatty acids were iso-C15:0, anteiso-C15:0, iso-C17:0, and anteiso-C17:0. The strain showed a DNA G+C content of 39.9 mol%. DNA-DNA hybridization studies revealed that strain NIO-1130(T) exhibits 70% similarity with Bacillus halosaccharovorans DSM 25387(T) and Bacillus niabensis CIP 109816(T). On the basis of physiological, biochemical, chemotaxonomic and phylogenetic analyses, we consider the isolate to represent a novel species of the genus Bacillus, for which the name Bacillus cellulasensis sp. nov., is proposed. The type strain is NIO-1130(T) (=NCIM 5461(T)=CCTCC AB 2011126(T)).
authors.affiliation: NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune, 411008, Maharashtra, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Mawlankar
authors.affiliation: NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune, 411008, Maharashtra, India.
authors.forename: Meghana N
authors.initials: MN
authors.lastname: Thorat
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh, 160036, India.
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Krishnamurthi
authors.affiliation: NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune, 411008, Maharashtra, India. sg.dastager@ncl.res.in.
authors.forename: Syed G
authors.initials: SG
authors.lastname: Dastager
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D007194:India; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
keywords: Bacillus sp; Cellulase; Marine sediment; Polyphasic taxonomy