title: Dual loading miR-218 mimics and Temozolomide using AuCOOH@FA-CS drug delivery system: promising targeted anti-tumor drug delivery system with sequential release functions.
abstract: BACKGROUND
  Dual loading drug delivery system with tumor targeting efficacy and sequential release function provides a promising platform for anticancer drug delivery. Herein, we established a novel AuCOOH@FACS nanogel system for co-delivery miR-218 mimics (as bio-drug) and Temozolomide(as chemo-drug).
  
  
  METHODS
  DLS and TEM were employed to determine the characteristics of particles and nanogels. The cell viability was calculated for study synergistic effect of both drugs coadministration and in nanogel forms. The amounts of Au uptake were measured by ICP-MS in cell and tumors to quantify the targeting drug delivery efficacy. Tumor weight and mice weight were investigated to study the targeting antitumor efficacy of nanogel system.
  
  
  RESULTS
  The results revealed that using AuCOOH@FACS nanogel as delivery vehicles, drugs could be targeting delivery to tumor site, the intracellular uptake is enhanced to a greater extent, and significant antitumor efficacy is fold increase compared with free drug administration group, without noticeable system cytotoxicity.
  
  
  CONCLUSIONS
  This system offers an efficient approach to cancer therapy and holds significant potential to improve the treatment of cancer in the future.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fourth Military Medical University, Xian, Shaanxi, 710032, China. xxfanny@fmmu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.affiliation: Institute of Materia Medica, School of Pharmacy, The Fourth Military Medical University, Xian, Shaanxi, 710032, China. 12154521@qq.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Department of Orthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, 510055, China. jasminenov@163.com.
authors.forename: Jiali
authors.initials: J
authors.lastname: Tan
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fourth Military Medical University, Xian, Shaanxi, 710032, China. youbei08@qq.com.
authors.forename: Youbei
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy Fourth Military Medical University, Xian, Shaanxi, 710032, China.
authors.forename: Qiaofeng
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fourth Military Medical University, Xian, Shaanxi, 710032, China. 365358549@qq.com.
authors.forename: Jingya
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fourth Military Medical University, Xian, Shaanxi, 710032, China. hongwuxa@hotmail.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Administrative, Tangdu hosipital, Fourth Military Medical University, Xian, Shaanxi, 710038, China. zhangys_td@163.com.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry; D003606:Dacarbazine / Q000031:analogs & derivatives* / Q000494:pharmacology; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems / Q000379:methods*; D005227:Fatty Acids / Q000737:chemistry; D005260:Female; D005909:Glioblastoma / Q000188:drug therapy; D017612:Gold Compounds / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000494:pharmacology*; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000494:pharmacology; D011094:Polyethyleneimine / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000737:chemistry; D000077204:Temozolomide; D023041:Xenograft Model Antitumor Assays
chemical_list: C505222:11-mercaptoundecanoic acid; D018906:Antineoplastic Agents, Alkylating; D003692:Delayed-Action Preparations; D005227:Fatty Acids; D017612:Gold Compounds; C547471:MIRN218 microRNA, human; D035683:MicroRNAs; D000080385:Nanogels; D013438:Sulfhydryl Compounds; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D003606:Dacarbazine; D011094:Polyethyleneimine; D048271:Chitosan; D000077204:Temozolomide