title: Rift Valley fever virus NSS gene expression correlates with a defect in nuclear mRNA export.
abstract: We investigated the localization of host mRNA during Rift Valley fever virus (RVFV) infection. Fluorescence in situ hybridization revealed that infection with RVFV altered the localization of host mRNA. mRNA accumulated in the nuclei of RVFV-infected but not mock-infected cells. Further, overexpression of the NSS gene, but not the N, GN or NSM genes correlated with mRNA nuclear accumulation. Nuclear accumulation of host mRNA was not observed in cells infected with a strain of RVFV lacking the gene encoding NSS, confirming that expression of NSS is likely responsible for this phenomenon.
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Copeland
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA.
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Van Deusen
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA. Electronic address: Connie.s.schmaljohn.civ@mail.mil.
authors.forename: Connie S
authors.initials: CS
authors.lastname: Schmaljohn
mesh_terms: D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012295:Rift Valley Fever / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D012296:Rift Valley fever virus / Q000235:genetics / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D017361:Viral Nonstructural Proteins
keywords: NS(S); PABP1; RNA export; Rift Valley fever virus; mRNA