title: Production of optically pure L-lactic acid from lignocellulosic hydrolysate by using a newly isolated and D-lactate dehydrogenase gene-deficient Lactobacillus paracasei strain.
abstract: The use of lignocellulosic feedstock for lactic acid production with a difficulty is that the release of inhibitory compounds during the pretreatment process which inhibit the growth of microorganism. Thus we report a novel lactic acid bacterium, Lactobacillus paracasei 7 BL, that has a high tolerance to inhibitors and produced optically pure l-lactic acid after the interruption of ldhD gene. The strain 7 BL fermented glucose efficiently and showed high titer of l-lactic acid (215 g/l) by fed-batch strategy. In addition, 99 g/l of l-lactic acid with high yield (0.96 g/g) and productivity (2.25-3.23 g/l/h) was obtained by using non-detoxified wood hydrolysate. Rice straw hydrolysate without detoxification was also tested and yielded a productivity rate as high as 5.27 g/l/h. Therefore, L. paracasei 7 BL represents a potential method of l-lactic acid production from lignocellulosic biomass and has attractive application for industries.
authors.affiliation: Chemical Division, Institute of Nuclear Energy Research, Taoyuan, Taiwan, ROC.
authors.forename: Yang-Cheng
authors.initials: YC
authors.lastname: Kuo
authors.affiliation: Chemical Division, Institute of Nuclear Energy Research, Taoyuan, Taiwan, ROC.
authors.forename: Shuo-Fu
authors.initials: SF
authors.lastname: Yuan
authors.affiliation: Chemical Division, Institute of Nuclear Energy Research, Taoyuan, Taiwan, ROC.
authors.forename: Chun-An
authors.initials: CA
authors.lastname: Wang
authors.affiliation: Chemical Division, Institute of Nuclear Energy Research, Taoyuan, Taiwan, ROC.
authors.forename: Yin-Jung
authors.initials: YJ
authors.lastname: Huang
authors.affiliation: Chemical Division, Institute of Nuclear Energy Research, Taoyuan, Taiwan, ROC. Electronic address: glguo@iner.gov.tw.
authors.forename: Gia-Luen
authors.initials: GL
authors.lastname: Guo
authors.affiliation: Chemical Division, Institute of Nuclear Energy Research, Taoyuan, Taiwan, ROC.
authors.forename: Wen-Song
authors.initials: WS
authors.lastname: Hwang
mesh_terms: D061249:Batch Cell Culture Techniques; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation; D005818:Genetic Engineering / Q000379:methods; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D045022:Lactate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis*; D007778:Lactobacillus / Q000235:genetics* / Q000378:metabolism*; D014934:Wood / Q000737:chemistry
chemical_list: D019344:Lactic Acid; D045022:Lactate Dehydrogenases; C071195:D-lactate dehydrogenase; D005947:Glucose
keywords: Inhibitors; Lactobacillus paracasei; Lignocellulosic hydrolysate; Optical purity; l-Lactic acid