title: Isolation of Chloroplasts from Plant Protoplasts.
abstract: Chloroplasts can be isolated from higher plants directly following homogenization; however, the resulting yield, purity, and intactness are often low, necessitating a large amount of starting material. This protocol is optimized to produce a high yield of pure chloroplasts from isolated Arabidopsis protoplasts. The two-part method is a simple, scaled-down, and low-cost procedure that readily provides healthy mesophyll protoplasts, which are then ruptured to release intact chloroplasts. Chloroplasts isolated using this method are competent for use in biochemical, cellular, and molecular analyses.
authors.affiliation: School of Biological Sciences, University of Hong Kong, Pokfulam, Hong Kong SAR, China;
authors.forename: Shiu-Cheung
authors.initials: SC
authors.lastname: Lung
authors.affiliation: Department of Biology, Wilfrid Laurier University, Waterloo, Ontario N2L 3C5, Canada;
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Smith
authors.affiliation: Department of Biology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.
authors.forename: Simon D X
authors.initials: SD
authors.lastname: Chuong
mesh_terms: D017360:Arabidopsis; D002458:Cell Fractionation / Q000379:methods*; D002736:Chloroplasts; D011523:Protoplasts