title: miR-429 regulates alveolar macrophage inflammatory cytokine production and is involved in LPS-induced acute lung injury.
abstract: p38 MAPK (mitogen-activated protein kinase) is a critical regulator in lung inflammation. It can be inactivated by DUSP1 (dual-specificity phosphatase 1) which was identified as a putative target of miR-429. miR-429 mimics directly targeted to the 3'-UTR of the gene encoding DUSP1 may result in the translational attenuation of DUSP1. Moreover, the phosphorylation of p38 MAPK was prolonged after miR-429 mimic treatment. Additionally, miR-429 expression was sensitive to LPS (lipopolysaccharide) stimulation and the miR-429 mimics increased the production of pro-inflammatory cytokines. However, anti-miR-429 reduced the LPS-induced production of pro-inflammatory cytokines. These results provide direct evidence that miR-429 is involved in the LPS-induced inflammatory response. In parallel with miR-429, miR-200b and miR-200c, but not miR-200a or miR-141, shared similar effects. In vivo, LPS induced the expression of miR-429, miR-200b and miR-200c in lung. At the same time, inhibiting these miRNAs by anti-miRNAs attenuated the LPS-induced pulmonary inflammatory response and injury. These findings reveal that miR-429 possesses pro-inflammatory activities and may be a potential therapy target for LPS-induced lung injury.
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China 253477652@qq.com zhchmo@163.com.
authors.forename: Ji
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China Department of Anesthesiology, The Maternal and Child Health Hospital of Hunan Province, Changsha, Hunan 410008, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tang
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Tang
authors.affiliation: Paediatrics Department, The Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China.
authors.forename: Meimei
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China.
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China.
authors.forename: Jiazheng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, Life Science Research Center, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Chaoke
authors.initials: C
authors.lastname: Tang
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China.
authors.forename: Deming
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, Life Science Research Center, University of South China, Hengyang, Hunan 421001, China 253477652@qq.com zhchmo@163.com.
authors.forename: Zhongcheng
authors.initials: Z
authors.lastname: Mo
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000633:toxicity*; D016676:Macrophages, Alveolar / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D035683:MicroRNAs; D048051:p38 Mitogen-Activated Protein Kinases
keywords: cytokine; dual-specificity phosphatase 1; lipopolysaccharide; miR-429; mitogen-activated protein kinase