title: Bioprinted Scaffolds for Cartilage Tissue Engineering.
abstract: Researchers are focusing on bioprinting technology as a viable option to overcome current difficulties in cartilage tissue engineering. Bioprinting enables a three-dimensional (3-D), free-form, computer-designed structure using biomaterials, biomolecules, and/or cells. The inner and outer shape of a scaffold can be controlled by this technology with great precision. Here, we introduce a hybrid bioprinting technology that is a co-printing process of multiple materials including high-strength synthetic polymer and cell-laden hydrogel. The synthetic polymer provides mechanical support for shape maintenance and load bearing, while the hydrogel provides the biological environment for artificial cartilage regeneration. This chapter introduces the procedures for printing of a 3-D scaffold using our hybrid bioprinting technology and includes the source materials for preparation of 3-D printing.
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC, 27157, USA.
authors.forename: Hyun-Wook
authors.initials: HW
authors.lastname: Kang
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC, 27157, USA.
authors.forename: James J
authors.initials: JJ
authors.lastname: Yoo
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC, 27157, USA. aatala@wakehealth.edu.
authors.forename: Anthony
authors.initials: A
authors.lastname: Atala
mesh_terms: D000818:Animals; D062028:Bioprinting; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D019902:Chondrocytes / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D020219:Chondrogenesis; D020100:Hydrogels; D011108:Polymers / Q000737:chemistry*; D066330:Printing, Three-Dimensional; D011817:Rabbits; D044968:Regenerative Medicine / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D020100:Hydrogels; D011108:Polymers
keywords: Bioprinting; Cartilage; Cell printing; Chondrocyte; Fibrin gel; Polycaprolactone; Scaffold