title: Microwave-assisted extraction and a new determination method for total steroid saponins from Dioscorea zingiberensis C.H. Wright.
abstract: An efficient microwave-assisted extraction (MAE) technique was applied to isolate total steroid saponins from Dioscorea zingiberensis C.H. Wright (DZW). The optimal extracting conditions were established as 75% ethanol as solvent, ratio of solid/liquid 1:20 (g/ml), temperature 75 °C, irradiation power 600 W and three extraction cycles of 6 min each. Scanning electron microscopy (SEM) images of DZW processed by four different extractions provided visual evidence of the disruption effect on DZW. Diosgenin was quantified by HPLC and examined further by LC-ESI/MS after acid hydrolysis. Total steroid saponins were calculated using diosgenin from total steroid saponins. The MAE procedure was optimized, validated and compared with other conventional extraction processes. This report provides a convenient technology for the extraction and quantification of total saponins of DZW combining MAE with HPLC and LC-ESI/MS for the first time.
authors.affiliation: College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China; College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Ren
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China.
authors.forename: Bohan
authors.initials: B
authors.lastname: Hu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China. Electronic address: fehwu@scut.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China.
authors.forename: Furao
authors.initials: F
authors.lastname: Lai
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China. Electronic address: xflibio@scut.edu.cn.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid; D027742:Dioscorea / Q000737:chemistry*; D006868:Hydrolysis; D008872:Microwaves; D008968:Molecular Conformation; D010316:Particle Size; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification*; D013499:Surface Properties
chemical_list: D012503:Saponins; D013256:Steroids
keywords: Acid hydrolysis; Dioscorea zingiberensis C.H. Wright; Diosgenin; Microwave-assisted extraction; Total steroid saponins