title: PGC-1α-mediated changes in phospholipid profiles of exercise-trained skeletal muscle.
abstract: Exercise training influences phospholipid fatty acid composition in skeletal muscle and these changes are associated with physiological phenotypes; however, the molecular mechanism of this influence on compositional changes is poorly understood. Peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), a nuclear receptor coactivator, promotes mitochondrial biogenesis, the fiber-type switch to oxidative fibers, and angiogenesis in skeletal muscle. Because exercise training induces these adaptations, together with increased PGC-1α, PGC-1α may contribute to the exercise-mediated change in phospholipid fatty acid composition. To determine the role of PGC-1α, we performed lipidomic analyses of skeletal muscle from genetically modified mice that overexpress PGC-1α in skeletal muscle or that carry KO alleles of PGC-1α. We found that PGC-1α affected lipid profiles in skeletal muscle and increased several phospholipid species in glycolytic muscle, namely phosphatidylcholine (PC) (18:0/22:6) and phosphatidylethanolamine (PE) (18:0/22:6). We also found that exercise training increased PC (18:0/22:6) and PE (18:0/22:6) in glycolytic muscle and that PGC-1α was required for these alterations. Because phospholipid fatty acid composition influences cell permeability and receptor stability at the cell membrane, these phospholipids may contribute to exercise training-mediated functional changes in the skeletal muscle.
authors.affiliation: Laboratories of Nutritional Biochemistry Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Suruga-ku, Shizuoka 422-8526, Japan.
authors.forename: Nanami
authors.initials: N
authors.lastname: Senoo
authors.affiliation: Biochemistry, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Suruga-ku, Shizuoka 422-8526, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Miyoshi
authors.affiliation: Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Goto-Inoue
authors.affiliation: Laboratory of Molecular Nutrition, Graduate School of Environmental and Life Science, Kyoto Prefectural University, Sakyo-ku, Kyoto, 606-8522, Japan.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Minami
authors.affiliation: Laboratory of Molecular Nutrition, Graduate School of Environmental and Life Science, Kyoto Prefectural University, Sakyo-ku, Kyoto, 606-8522, Japan.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Yoshimura
authors.affiliation: Laboratories of Nutritional Biochemistry Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Suruga-ku, Shizuoka 422-8526, Japan.
authors.forename: Akihito
authors.initials: A
authors.lastname: Morita
authors.affiliation: Section of Cardiology, Department of Medicine, University of Chicago, Chicago, IL 60637 Department of Molecular Endocrinology and Metabolism, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8510, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Sawada
authors.affiliation: Laboratory of Animal Models for Human Diseases, National Institutes of Biomedical Innovation, Health, and Nutrition, Ibaraki, Osaka 567-0085, Japan.
authors.forename: Junichiro
authors.initials: J
authors.lastname: Matsuda
authors.affiliation: Department of Molecular Endocrinology and Metabolism, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8510, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ogawa
authors.affiliation: Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan Department of Anatomy, University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
authors.affiliation: Laboratory of Molecular Nutrition, Graduate School of Environmental and Life Science, Kyoto Prefectural University, Sakyo-ku, Kyoto, 606-8522, Japan miura@u-shizuoka-ken.ac.jp kamei@kpu.ac.jp.
authors.forename: Yasutomi
authors.initials: Y
authors.lastname: Kamei
authors.affiliation: Laboratories of Nutritional Biochemistry Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Suruga-ku, Shizuoka 422-8526, Japan miura@u-shizuoka-ken.ac.jp kamei@kpu.ac.jp.
authors.forename: Shinji
authors.initials: S
authors.lastname: Miura
mesh_terms: D000818:Animals; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000378:metabolism*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010743:Phospholipids / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010743:Phospholipids; D014157:Transcription Factors
keywords: biochemical assays; fatty acid; lipidomics; mass spectrometry; molecular imaging; nuclear receptors; peroxisome proliferator-activated receptor γ coactivator 1α; phospholipids/phosphatidylcholine; phospholipids/phosphatidylethanolamine; transcription factors