title: High-Throughput Development of SSR Markers from Pea (Pisum sativum L.) Based on Next Generation Sequencing of a Purified Chinese Commercial Variety.
abstract: Pea (Pisum sativum L.) is an important food legume globally, and is the plant species that J.G. Mendel used to lay the foundation of modern genetics. However, genomics resources of pea are limited comparing to other crop species. Application of marker assisted selection (MAS) in pea breeding has lagged behind many other crops. Development of a large number of novel and reliable SSR (simple sequence repeat) or microsatellite markers will help both basic and applied genomics research of this crop. The Illumina HiSeq 2500 System was used to uncover 8,899 putative SSR containing sequences, and 3,275 non-redundant primers were designed to amplify these SSRs. Among the 1,644 SSRs that were randomly selected for primer validation, 841 yielded reliable amplifications of detectable polymorphisms among 24 genotypes of cultivated pea (Pisum sativum L.) and wild relatives (P. fulvum Sm.) originated from diverse geographical locations. The dataset indicated that the allele number per locus ranged from 2 to 10, and that the polymorphism information content (PIC) ranged from 0.08 to 0.82 with an average of 0.38. These 1,644 novel SSR markers were also tested for polymorphism between genotypes G0003973 and G0005527. Finally, 33 polymorphic SSR markers were anchored on the genetic linkage map of G0003973 × G0005527 F2 population.
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Fang
authors.affiliation: Qingdao Academy of Agricultural Sciences, Qingdao, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: USDA-ARS Western Regional Plant Introduction Station, Pullman, Washington, United States of America.
authors.forename: Jinguo
authors.initials: J
authors.lastname: Hu
authors.affiliation: Institute of Grain Crops, Yunnan Academy of Agricultural Sciences, Kunming, China.
authors.forename: Shiying
authors.initials: S
authors.lastname: Bao
authors.affiliation: Qingdao Academy of Agricultural Sciences, Qingdao, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Hao
authors.affiliation: Institute of Cash Crops, Liaoning Academy of Agricultural Sciences, Liaoyang, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Grain Crops, Yunnan Academy of Agricultural Sciences, Kunming, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: He
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Junye
authors.initials: J
authors.lastname: Jiang
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Tian
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Xuxiao
authors.initials: X
authors.lastname: Zong
mesh_terms: D000483:Alleles; D018745:Genome, Plant; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing; D018895:Microsatellite Repeats; D018532:Pisum sativum / Q000235:genetics*; D011110:Polymorphism, Genetic