title: Advances in DNA sequencing technologies for high resolution HLA typing.
abstract: This communication describes our experience in large-scale G group-level high resolution HLA typing using three different DNA sequencing platforms - ABI 3730 xl, Illumina MiSeq and PacBio RS II. Recent advances in DNA sequencing technologies, so-called next generation sequencing (NGS), have brought breakthroughs in deciphering the genetic information in all living species at a large scale and at an affordable level. The NGS DNA indexing system allows sequencing multiple genes for large number of individuals in a single run. Our laboratory has adopted and used these technologies for HLA molecular testing services. We found that each sequencing technology has its own strengths and weaknesses, and their sequencing performances complement each other. HLA genes are highly complex and genotyping them is quite challenging. Using these three sequencing platforms, we were able to meet all requirements for G group-level high resolution and high volume HLA typing.
authors.affiliation: Histogenetics, 300 Executive Blvd, Ossining, NY 10562, USA. Electronic address: nezih@histogenetics.com.
authors.forename: Nezih
authors.initials: N
authors.lastname: Cereb
authors.affiliation: Histogenetics, 300 Executive Blvd, Ossining, NY 10562, USA.
authors.forename: Hwa Ran
authors.initials: HR
authors.lastname: Kim
authors.affiliation: Histogenetics, 300 Executive Blvd, Ossining, NY 10562, USA.
authors.forename: Jaejun
authors.initials: J
authors.lastname: Ryu
authors.affiliation: Histogenetics, 300 Executive Blvd, Ossining, NY 10562, USA.
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Yang
mesh_terms: D000483:Alleles; D019295:Computational Biology / Q000379:methods; D006680:HLA Antigens / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods* / Q000592:standards; D006650:Histocompatibility Testing; D006801:Humans; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA
chemical_list: D006680:HLA Antigens
keywords: DNA sequence-based HLA typing (SBT); HLA typing; MiSeq; NGS; New alleles; PacBio; Sanger sequencing