title: Effect of Vascular Endothelial Growth Factor Administration on Nerve Regeneration after Autologous Nerve Grafting.
abstract: BACKGROUND
  The aim of this study was to evaluate the effect of vascular endothelial growth factor (VEGF) administration around the autologous nerve graft on nerve recovery in a rat model.
  
  
  METHODS
  A total of 69 rats were randomly divided into three experimental groups. A 10-mm sciatic nerve defect was made and reconstructed with the reversed nerve segment. Group I received an osmotic pump with saline, group II received an osmotic pump with VEGF, and group III added a silicone tube around the nerve graft to decrease the surrounding blood supply. Nine animals in each group were sacrificed on day 3 to evaluate improvement in new vessel formation. In each group 14 animals were sacrificed at 16 weeks after the initial procedure to evaluate the functional motor nerve regeneration using compound muscle action potential, isometric tetanic force, wet muscle weight, and nerve histomorphometry.
  
  
  RESULTS
  The average vascular density on day 3 was 10.7% in group I, 21.4% in group II, and 0.9% in group III. These differences were significant. However, the average maximum isometric tetanic force at 16 weeks was 54.4% in group I, 57.5% in group II, and 47.6% in group III. No difference was found with or without VEGF administration. Histomorphometric analysis was also not significantly different between the groups.
  
  
  CONCLUSIONS
  New vessel formation on autologous nerve graft was enhanced by VEGF administration. However, the neovascularization effect of VEGF administration did not translate into better motor nerve recovery.
authors.affiliation: Department of Orthopedic Surgery, St. Vincent's Hospital, the Catholic University of Korea, Seoul, Korea.
authors.forename: Joo-Yup
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Microvascular Research Laboratory, Mayo Clinic, Rochester, Minnesota.
authors.forename: Guilherme
authors.initials: G
authors.lastname: Giusti
authors.affiliation: Microvascular Research Laboratory, Mayo Clinic, Rochester, Minnesota.
authors.forename: Patricia F
authors.initials: PF
authors.lastname: Friedrich
authors.affiliation: Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota.
authors.forename: Allen T
authors.initials: AT
authors.lastname: Bishop
authors.affiliation: Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota.
authors.forename: Alexander Y
authors.initials: AY
authors.lastname: Shin
mesh_terms: D000200:Action Potentials; D000792:Angiography; D000818:Animals; D064592:Autografts; D018919:Neovascularization, Physiologic; D009416:Nerve Regeneration / Q000187:drug effects*; D019635:Neurosurgical Procedures; D011897:Random Allocation; D051381:Rats; D012584:Sciatic Nerve / Q000098:blood supply / Q000601:surgery*; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
chemical_list: D042461:Vascular Endothelial Growth Factor A