title: Nuclear Reprogramming by Defined Factors: Quantity Versus Quality.
abstract: The generation of induced pluripotent stem cells (iPSCs) and directly converted cells holds great promise in regenerative medicine. However, after in-depth studies of the murine system, we know that the current methodologies to produce these cells are not ideal and mostly yield cells of poor quality that might hold a risk in therapeutic applications. In this review we address the duality found in the literature regarding the use of 'quality' as a criterion for the clinic. We discuss the elements that influence reprogramming quality, and provide evidence that safety and functionality are directly linked to cell quality. Finally, because most of the available data come from murine systems, we speculate about what aspects can be applied to human cells.
authors.affiliation: Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
authors.forename: Shulamit
authors.initials: S
authors.lastname: Sebban
authors.affiliation: Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel. Electronic address: yossibug@ekmd.huji.ac.il.
authors.forename: Yosef
authors.initials: Y
authors.lastname: Buganim
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology; D044968:Regenerative Medicine; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D014157:Transcription Factors
keywords: direct conversion; epigenetics; induced pluripotent stem cells (iPSCs); nuclear reprogramming; regenerative medicine; transdifferentiation