title: SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy.
abstract: Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology, 1015 Lausanne, Switzerland.
authors.forename: Gražvydas
authors.initials: G
authors.lastname: Lukinavičius
authors.affiliation: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter Campus (VBC), Dr Bohr Gasse 3, 1030 Vienna, Austria.
authors.forename: Claudia
authors.initials: C
authors.lastname: Blaukopf
authors.affiliation: Department of Chemistry-BMC, Uppsala University, 75123 Uppsala, Sweden.
authors.forename: Elias
authors.initials: E
authors.lastname: Pershagen
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology, 1015 Lausanne, Switzerland.
authors.forename: Alberto
authors.initials: A
authors.lastname: Schena
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology, 1015 Lausanne, Switzerland.
authors.forename: Luc
authors.initials: L
authors.lastname: Reymond
authors.affiliation: Department of Biochemistry, NCCR in Chemical Biology, University of Geneva, 1211 Geneva, Switzerland.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Derivery
authors.affiliation: Department of Biochemistry, NCCR in Chemical Biology, University of Geneva, 1211 Geneva, Switzerland.
authors.forename: Marcos
authors.initials: M
authors.lastname: Gonzalez-Gaitan
authors.affiliation: Department NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Elisa
authors.initials: E
authors.lastname: D'Este
authors.affiliation: Department NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
authors.affiliation: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter Campus (VBC), Dr Bohr Gasse 3, 1030 Vienna, Austria.
authors.forename: Daniel
authors.initials: D
authors.lastname: Wolfram Gerlich
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology, 1015 Lausanne, Switzerland.
authors.forename: Kai
authors.initials: K
authors.lastname: Johnsson
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000737:chemistry*; D004330:Drosophila; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008853:Microscopy / Q000379:methods; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods; D015394:Molecular Structure; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D004247:DNA