title: Organogel-nanoemulsion containing nisin and D-limonene and its antimicrobial activity.
abstract: The aim of this study was to investigate a novel delivery system containing D-limonene and nisin by food organogel-nanoemulsion and study its effect on the antimicrobial activity. Organogel-nanoemulsion containing with D-limonene and nisin or without nisin was prepared by a homogenization method. Factors that may affect the droplet size and stability of organogel-nanoemulsion such as pressure and surfactant to oil ratio (SOR) were studied. The average droplet size decreased with pressure, and the organogel-nanoemulsion could achieve good stability at low SOR. Positive effects and outstanding antimicrobial activities of organogel-nanoemulsion containing with D-limonene and nisin were confirmed by minimal inhibitory concentrations comparison, growth curves of bacteria, scanning electron microscopy and determination of cell constituents' release. Furthermore, the organogel-nanoemulsion applied as food preservative in milk also shown excellent antimicrobial performance. Overall, the research described in the current article show that organogel-nanoemulsion containing with D-limonene and nisin may be an effective antimicrobial system for the production and preservation of food.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology Beijing, China.
authors.forename: Weiya
authors.initials: W
authors.lastname: Bei
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology Beijing, China.
authors.forename: Xuya
authors.initials: X
authors.lastname: Xing
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology Beijing, China.
authors.forename: Mohamed Reda
authors.initials: MR
authors.lastname: Zahi
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology Beijing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology Beijing, China.
authors.forename: Qipeng
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology Beijing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
keywords: D-limonene; antimicrobial activity; food preservation; nisin; organogel-nanoemulsion