title: Prevalence of internal parasites in beef cows in the United States: Results of the National Animal Health Monitoring System's (NAHMS) beef study, 2007-2008.
abstract: During the United States Department of Agriculture (USDA) National Animal Health Monitoring System's (NAHMS) 2007-2008 beef study, 567 producers from 24 US States were offered the opportunity to collect fecal samples from weaned beef calves and have them evaluated for the presence of parasite eggs (Phase 1). Participating producers were provided with instructions and materials for sample collection. Up to 20 fresh fecal samples were collected from each of the 99 participating operations. Fresh fecal samples were submitted to one of 3 randomly assigned laboratories for evaluation. Upon arrival at the laboratories, all samples were processed for the enumeration of strongyle, Nematodirus, and Trichuris eggs using the modified Wisconsin technique. The presence or absence of coccidian oocysts and tapeworm eggs was also noted. In submissions where the strongyle eggs per gram exceeded 30, aliquots from 2 to 6 animals were pooled for DNA extraction. Extracted DNA was subjected to genus level polymerase chain reaction (PCR) identification for the presence of Ostertagia, Cooperia, Haemonchus, Oesophagostomum, and Trichostrongylus. In this study, 85.6% of the samples had strongyle type, Nematodirus, and Trichuris eggs. Among the samples evaluated, 91% had Cooperia, 79% Ostertagia, 53% Haemonchus, 38% Oesophagostomum, 18% Nematodirus, 7% Trichuris, and 3% Trichostrongylus. The prevalence of coccidia and tapeworm eggs was 59.9% and 13.7%, respectively.
authors.affiliation: College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota (Stromberg); United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Bovine Functional Genomics Lab, Beltsville, Maryland (Gasbarre); Colorado State University, Veterinary Diagnostic Laboratory, Fort Collins, Colorado (Ballweber); USDA, Animal and Plant Health Inspection Service (APHIS), Veterinary Service (VS) Centers for Epidemiology and Animal Health, Fort Collins, Colorado (Dargatz, Rodriguez, Kopral); USDA, ARS, Animal Parasitic Diseases Lab, Beltsville, Maryland, USA (Zarlenga).
authors.forename: Bert E
authors.initials: BE
authors.lastname: Stromberg
authors.affiliation: College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota (Stromberg); United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Bovine Functional Genomics Lab, Beltsville, Maryland (Gasbarre); Colorado State University, Veterinary Diagnostic Laboratory, Fort Collins, Colorado (Ballweber); USDA, Animal and Plant Health Inspection Service (APHIS), Veterinary Service (VS) Centers for Epidemiology and Animal Health, Fort Collins, Colorado (Dargatz, Rodriguez, Kopral); USDA, ARS, Animal Parasitic Diseases Lab, Beltsville, Maryland, USA (Zarlenga).
authors.forename: Louis C
authors.initials: LC
authors.lastname: Gasbarre
authors.affiliation: College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota (Stromberg); United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Bovine Functional Genomics Lab, Beltsville, Maryland (Gasbarre); Colorado State University, Veterinary Diagnostic Laboratory, Fort Collins, Colorado (Ballweber); USDA, Animal and Plant Health Inspection Service (APHIS), Veterinary Service (VS) Centers for Epidemiology and Animal Health, Fort Collins, Colorado (Dargatz, Rodriguez, Kopral); USDA, ARS, Animal Parasitic Diseases Lab, Beltsville, Maryland, USA (Zarlenga).
authors.forename: Lora R
authors.initials: LR
authors.lastname: Ballweber
authors.affiliation: College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota (Stromberg); United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Bovine Functional Genomics Lab, Beltsville, Maryland (Gasbarre); Colorado State University, Veterinary Diagnostic Laboratory, Fort Collins, Colorado (Ballweber); USDA, Animal and Plant Health Inspection Service (APHIS), Veterinary Service (VS) Centers for Epidemiology and Animal Health, Fort Collins, Colorado (Dargatz, Rodriguez, Kopral); USDA, ARS, Animal Parasitic Diseases Lab, Beltsville, Maryland, USA (Zarlenga).
authors.forename: David A
authors.initials: DA
authors.lastname: Dargatz
authors.affiliation: College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota (Stromberg); United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Bovine Functional Genomics Lab, Beltsville, Maryland (Gasbarre); Colorado State University, Veterinary Diagnostic Laboratory, Fort Collins, Colorado (Ballweber); USDA, Animal and Plant Health Inspection Service (APHIS), Veterinary Service (VS) Centers for Epidemiology and Animal Health, Fort Collins, Colorado (Dargatz, Rodriguez, Kopral); USDA, ARS, Animal Parasitic Diseases Lab, Beltsville, Maryland, USA (Zarlenga).
authors.forename: Judith M
authors.initials: JM
authors.lastname: Rodriguez
authors.affiliation: College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota (Stromberg); United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Bovine Functional Genomics Lab, Beltsville, Maryland (Gasbarre); Colorado State University, Veterinary Diagnostic Laboratory, Fort Collins, Colorado (Ballweber); USDA, Animal and Plant Health Inspection Service (APHIS), Veterinary Service (VS) Centers for Epidemiology and Animal Health, Fort Collins, Colorado (Dargatz, Rodriguez, Kopral); USDA, ARS, Animal Parasitic Diseases Lab, Beltsville, Maryland, USA (Zarlenga).
authors.forename: Christine A
authors.initials: CA
authors.lastname: Kopral
authors.affiliation: College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota (Stromberg); United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Bovine Functional Genomics Lab, Beltsville, Maryland (Gasbarre); Colorado State University, Veterinary Diagnostic Laboratory, Fort Collins, Colorado (Ballweber); USDA, Animal and Plant Health Inspection Service (APHIS), Veterinary Service (VS) Centers for Epidemiology and Animal Health, Fort Collins, Colorado (Dargatz, Rodriguez, Kopral); USDA, ARS, Animal Parasitic Diseases Lab, Beltsville, Maryland, USA (Zarlenga).
authors.forename: Dante S
authors.initials: DS
authors.lastname: Zarlenga
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D003625:Data Collection; D005260:Female; D006374:Helminthiasis, Animal / Q000453:epidemiology / Q000469:parasitology*; D009313:National Health Programs / Q000706:statistics & numerical data*; D015995:Prevalence; D011795:Surveys and Questionnaires; D014481:United States; D016885:United States Department of Agriculture