title: Pfaffia paniculata extract improves red blood cell deformability in sickle cell patients.
abstract: The aim of the present study was to test the effects of Pfaffia paniculata (PP) extract on the red blood cell (RBC) rheological properties of patients with sickle cell disease (SCD) and healthy (AA) individuals. Blood from 7 SCD and 4 AA individuals were collected in EDTA tubes. Washed RBCs were incubated with various concentration of PP extract: 0.0, 0.2 or 0.5 mg/ml of PP solution for 5 hrs at 37°C. RBC deformability was measured by ektacytometry at 9 shear stresses ranging from 0.3 to 30 Pa, and RBC aggregation properties were determined by laser-backscattered techniques. Because RBCs from SCD patients are fragile, a stability test was also performed to test for the fragility of RBC exposed to a constant shear stress (70 Pa) for 10 min. While RBC deformability was not improved by the use of PP extract in AA, we noted an improvement of this parameter in patients with SCD between the 0.0 and 0.5 mg/ml conditions. In contrast to AA RBCs, the fragility of SCD RBCs was not affected by PP extract. In conclusion, this study demonstrates the beneficial effects, in-vitro, of PP extract on the RBC deformability of SCD patients, notably at high shear stress (a shear stress condition usually found in capillaries).
authors.affiliation: Inserm U 1134, Université des Antilles et de la Guyane, Pointe-à-Pitre, Guadeloupe.|Laboratory of Excellence GR-Ex, Paris, France.
authors.forename: Anais
authors.initials: A
authors.lastname: Mozar
authors.affiliation: Inserm U 1134, Université des Antilles et de la Guyane, Pointe-à-Pitre, Guadeloupe.|Laboratory of Excellence GR-Ex, Paris, France.|Laboratoire ACTES-EA3596, Université des Antilles et de la Guyane, Pointe-à-Pitre, Guadeloupe.
authors.forename: Keyne
authors.initials: K
authors.lastname: Charlot
authors.affiliation: Department of Medicine, University of Pecs Medical School, Pecs, Hungary.
authors.forename: Barbara
authors.initials: B
authors.lastname: Sandor
authors.affiliation: Department of Medicine, University of Pecs Medical School, Pecs, Hungary.
authors.forename: Miklos
authors.initials: M
authors.lastname: Rabaï
authors.affiliation: Unité Transversale de la Drépanocytose, CHU de Pointe-à-Pitre, Pointe-à-Pitre, Guadeloupe.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Lemonne
authors.affiliation: Unité Transversale de la Drépanocytose, CHU de Pointe-à-Pitre, Pointe-à-Pitre, Guadeloupe.
authors.forename: Marie
authors.initials: M
authors.lastname: Billaud
authors.affiliation: Inserm U 1134, Université des Antilles et de la Guyane, Pointe-à-Pitre, Guadeloupe.|Laboratory of Excellence GR-Ex, Paris, France.
authors.forename: Marie-Dominique
authors.initials: MD
authors.lastname: Hardy-Dessources
authors.affiliation: Service d'Immunologie-Hématologie, CHU de Pointe à Pitre, Pointe-à-Pitre, Guadeloupe.
authors.forename: Eric
authors.initials: E
authors.lastname: Beltan
authors.affiliation: Research Laboratories, GDP Ayurvedic University (GDPAU), New Brunswick, NJ, USA.
authors.forename: Ramesh C
authors.initials: RC
authors.lastname: Pandey
authors.affiliation: Inserm U 1134, Université des Antilles et de la Guyane, Pointe-à-Pitre, Guadeloupe.|Laboratory of Excellence GR-Ex, Paris, France.|Institut Universitaire de France, Paris, France.|Laboratoire CRIS EA647, Equipe "Biologie Vasculaire et Globule Rouge", Université de Lyon (UCBL1), France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Connes
authors.affiliation: Cardeza Foundation, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA, USA.
authors.forename: Samir K
authors.initials: SK
authors.lastname: Ballas
mesh_terms: D000755:Anemia, Sickle Cell / Q000097:blood*; D004907:Erythrocyte Deformability / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects*; D005260:Female; D006801:Humans; D008297:Male; D010936:Plant Extracts / Q000627:therapeutic use*; D012212:Rheology
chemical_list: D010936:Plant Extracts
keywords: Pfaffia paniculata; blood rheology; sickle cell anemia; sickle cell disease