title: Virus-resembling nano-structures for near infrared fluorescence imaging of ovarian cancer HER2 receptors.
abstract: Ovarian cancer remains the dominant cause of death due to malignancies of the female reproductive system. The capability to identify and remove all tumors during intraoperative procedures may ultimately reduce cancer recurrence, and lead to increased patient survival. The objective of this study is to investigate the effectiveness of an optical nano-structured system for targeted near infrared (NIR) imaging of ovarian cancer cells that over-express the human epidermal growth factor receptor 2 (HER2), an important biomarker associated with ovarian cancer. The nano-structured system is comprised of genome-depleted plant-infecting brome mosaic virus doped with NIR chromophore, indocyanine green, and functionalized at the surface by covalent attachment of monoclonal antibodies against the HER2 receptor. We use absorption and fluorescence spectroscopy, and dynamic light scattering to characterize the physical properties of the constructs. Using fluorescence imaging and flow cytometry, we demonstrate the effectiveness of these nano-structures for targeted NIR imaging of HER2 receptors in vitro. These functionalized nano-materials may provide a platform for NIR imaging of ovarian cancer.
authors.affiliation: Department of Bioengineering, University of California, Riverside, CA 92521, USA.
authors.forename: Yadir A
authors.initials: YA
authors.lastname: Guerrero
authors.forename: Baharak
authors.initials: B
authors.lastname: Bahmani
authors.forename: Sheela P
authors.initials: SP
authors.lastname: Singh
authors.forename: Valentine I
authors.initials: VI
authors.lastname: Vullev
authors.forename: Vikas
authors.initials: V
authors.lastname: Kundra
authors.forename: Bahman
authors.initials: B
authors.lastname: Anvari
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D014408:Biomarkers, Tumor / Q000378:metabolism; D017795:Bromovirus / Q000502:physiology*; D045744:Cell Line, Tumor; D000067493:Dynamic Light Scattering; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D007208:Indocyanine Green / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D019265:Spectroscopy, Near-Infrared
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D016650:Fluorescein-5-isothiocyanate; D007208:Indocyanine Green; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human