title: TIIA attenuates LPS-induced mouse endometritis by suppressing the NF-κB signaling pathway.
abstract: Endometritis is one of the main diseases that harms the dairy cow industry. Tanshinone IIA (TIIA), a fat-soluble alkaloid isolated from Salviae miltiorrhizae, has been reported to have potent anti-inflammatory properties. However, the anti-inflammatory effects of TIIA on a mouse model of lipopolysaccharide (LPS)-induced endometritis remain to be elucidated. The purpose of the present study was to investigate the effects of TIIA on LPS-induced mouse endometritis. TIIA was intraperitoneally injected 1 h before and 12 h after perfusion of LPS into the uterus. A histological examination was then performed, and the concentrations of myeloperoxidase (MPO) and nitric oxide (NO) in the uterine tissue were determined. The levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in a homogenate of the uterus were detected by enzyme-linked immunosorbent assay. The extent of phosphorylation of IκBα and p65 was detected by Western blotting. TIIA markedly reduced the infiltration of neutrophils, suppressed MPO activity and the concentration of NO, and attenuated the expression of TNF-α and IL-1β. Furthermore, TIIA inhibited the phosphorylation of the nuclear factor-kappa B (NF-κB) p65 subunit and the degradation of its inhibitor IκBα. All the results suggest that TIIA has strong anti-inflammatory effects on LPS-induced mouse endometritis.
authors.affiliation: Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.|Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.
authors.forename: Xiaopei
authors.initials: X
authors.lastname: Lv
authors.affiliation: Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.|Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.
authors.forename: Kaiqiang
authors.initials: K
authors.lastname: Fu
authors.affiliation: Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.|Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.
authors.forename: Weishi
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.|Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.|Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.
authors.forename: Jifang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.|Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.
authors.forename: Huatao
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.|Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.
authors.forename: Wenru
authors.initials: W
authors.lastname: Tian
authors.affiliation: Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.|Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province 266109, People's Republic of China.
authors.forename: Rongfeng
authors.initials: R
authors.lastname: Cao
mesh_terms: D045784:Abietanes / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use; D004716:Endometritis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D005260:Female; D008070:Lipopolysaccharides / Q000633:toxicity*; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
chemical_list: D045784:Abietanes; D000894:Anti-Inflammatory Agents, Non-Steroidal; D008070:Lipopolysaccharides; D016328:NF-kappa B; C021751:tanshinone
keywords: LPS; NF-κB; TIIA; endometritis; endométrite