title: Simultaneous MR imaging for tissue engineering in a rat model of stroke.
abstract: In situ tissue engineering within a stroke cavity is gradually emerging as a novel therapeutic paradigm. Considering the varied lesion topology within each subject, the placement and distribution of cells within the lesion cavity is challenging. The use of multiple cell types to reconstruct damaged tissue illustrates the complexity of the process, but also highlights the challenges to provide a non-invasive assessment. The distribution of implanted cells within the lesion cavity and crucially the contribution of neural stem cells and endothelial cells to morphogenesis could be visualized simultaneously using two paramagnetic chemical exchange saturation transfer (paraCEST) agents. The development of sophisticated imaging methods is essential to guide delivery of the building blocks for in situ tissue engineering, but will also be essential to understand the dynamics of cellular interactions leading to the formation of de novo tissue.
authors.affiliation: Department of Radiology, Pittsburgh, PA.|McGowan Institute for Regenerative Medicine and Pittsburgh, PA.|Institute of Psychiatry, Psychology and Neuroscience, King's College London, UK.
authors.forename: Francesca J
authors.initials: FJ
authors.lastname: Nicholls
authors.affiliation: Department of Radiology, Pittsburgh, PA.
authors.forename: Wen
authors.initials: W
authors.lastname: Ling
authors.affiliation: Molecular Imaging Center, Department of Chemistry, University of Torino, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ferrauto
authors.affiliation: Molecular Imaging Center, Department of Chemistry, University of Torino, Italy.
authors.forename: Silvio
authors.initials: S
authors.lastname: Aime
authors.affiliation: Department of Radiology, Pittsburgh, PA.|McGowan Institute for Regenerative Medicine and Pittsburgh, PA.|Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA.
authors.forename: Michel
authors.initials: M
authors.lastname: Modo
mesh_terms: D000818:Animals; D058948:Cell Tracking / Q000379:methods; D064987:Cell- and Tissue-Based Therapy; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D010873:Pinocytosis; D051381:Rats; D013194:Staining and Labeling; D033581:Stem Cell Transplantation; D020521:Stroke / Q000175:diagnosis / Q000473:pathology* / Q000628:therapy; D023822:Tissue Engineering / Q000379:methods