title: Purification and Injection of Retroviral Vectors.
abstract: Retroviral vectors are powerful tools for genetic manipulation. This protocol discusses the production, purification, and use of replication-deficient retroviral vectors based on Moloney murine leukemia virus and lentivirus. It also describes the injection of a retroviral vector into the dentate gyrus of young adult mice to fluorescently label live murine brain tissue.
authors.forename: Ayumu
authors.initials: A
authors.lastname: Tashiro
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhao
authors.forename: Hoonkyo
authors.initials: H
authors.lastname: Suh
authors.forename: Fred H
authors.initials: FH
authors.lastname: Gage
mesh_terms: D000818:Animals; D005822:Genetic Vectors / Q000302:isolation & purification*; D016086:Lentivirus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D014779:Virus Replication