title: Multicolor Digital Flow Cytometry in Human Translational Immunology.
abstract: By facilitating the simultaneous analysis of parameters from diverse cell lineages and biological pathways, multicolor flow cytometry is integral to many studies in human immunology-particularly those in older individuals-where sample amounts may be limiting. Studies in human cohorts require particular attention to fluorochrome panel design and procedures to standardize instrument performance; reproducible instrument conditions (over time and between centers) are crucial to accurate comparisons and conclusions in the analysis of heterogeneous groups of human subjects. Here, we describe procedures for multicolor digital flow cytometry, our experience in flow cytometry panel design and our approach in standardizing instrument performance using BD Biosciences hardware and software (BD Biosciences, San Jose, CA). These techniques allow for the generation of accurate and precise data in a variety of settings.
authors.affiliation: Section of Infectious Diseases, Department of Internal Medicine, Yale School of Medicine, 300 Cedar St., 208056, New Haven, CT, 06520, USA. samit.joshi@yale.edu.|Bristol-Myers Squibb, Inc, 5 Research Parkway, Wallingford, CT, 06492, USA. samit.joshi@yale.edu.
authors.forename: Samit R
authors.initials: SR
authors.lastname: Joshi
authors.affiliation: Section of Infectious Diseases, Department of Internal Medicine, Yale School of Medicine, 300 Cedar St., 208056, New Haven, CT, 06520, USA.
authors.forename: Subhasis
authors.initials: S
authors.lastname: Mohanty
authors.affiliation: Section of Infectious Diseases, Department of Internal Medicine, Yale School of Medicine, 300 Cedar St., 208056, New Haven, CT, 06520, USA. albert.shaw@yale.edu.
authors.forename: Albert C
authors.initials: AC
authors.lastname: Shaw
mesh_terms: D000906:Antibodies; D005434:Flow Cytometry / Q000379:methods* / Q000592:standards; D005455:Fluorescent Antibody Technique; D006801:Humans; D007158:Immunologic Techniques; D057170:Translational Research, Biomedical / Q000379:methods* / Q000592:standards
chemical_list: D000906:Antibodies
keywords: Application settings; Flow cytometry panel design; Flow cytometry standardization; Multicolor flow cytometry; Translational immunology