title: Morphological characteristics of the sinus node on postmortem tissue.
abstract: The sinus node is an intensively researched structure in terms of anatomical, histological, electrophysiological, molecular and genetic approach. For postmortem diagnosis it is still difficult to investigate due to a still reduced accessibility. In this study we tried and succeed to apply molecular biology techniques on postmortem tissues in order to widen the range of postmortem forensic investigation and provide information related to the diagnostic of cardiac arrhythmia. We described the stages of this investigation, with dissection, preservation and analysis that included classical histology, immunohistochemistry, confocal microscope, microdissection, RIN testing, mRNA expression obtaining a precise morphofunctional location of the sinus node.
authors.affiliation: Prosecture Department, National Institute of Legal Medicine, Bucharest, Romania. filipvirgil@gmail.com.
authors.forename: F V
authors.initials: FV
authors.lastname: Perde
authors.forename: A
authors.initials: A
authors.lastname: Atkinson
authors.forename: J
authors.initials: J
authors.lastname: Yanni
authors.forename: D
authors.initials: D
authors.lastname: Dermengiu
authors.forename: H
authors.initials: H
authors.lastname: Dobrzynski
mesh_terms: D001344:Autopsy; D007150:Immunohistochemistry; D011180:Postmortem Changes; D012849:Sinoatrial Node
keywords: autopsy; connexin 43; messenger RNA