title: Expression of stem cell-related genes in the endometrium and endometriotic lesions: a pilot study.
abstract: OBJECTIVE
  To compare the expression of stem cell-related genes in the endometrium (END), superficial endometriosis (SE), and deep infiltrating endometriosis (DIE).
  
  
  METHODS
  We performed a prospective pilot study of six women suffering from SE and DIE who gave consent for laparoscopy surgery, endometrial biopsies, and participation in this study. Quantitative RT-PCR analysis of 84 stem cell-related genes was performed in 18 biopsy samples.
  
  
  RESULTS
  A total of 40 of 84 genes were expressed in SE and DIE, but were different from END as follows. Seven genes were over-expressed in SE and 33 genes were under-expressed in DIE compared with END. Two genes were only over-expressed in SE and three genes were only over-expressed in DIE. Six under-expressed genes were exclusively located in SE and one was only located in DIE. The remaining 31 genes were not different among the groups. There was no significant difference in gene expression between SE and DIE samples.
  
  
  CONCLUSIONS
  Tissue of DIE and SE appears to have similar stem cell-related genes. Nevertheless, there are differences in gene expression between SE and DIE.
authors.affiliation: a Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Faculdade de Medicina da Universidade de São Paulo, Hospital das Clínicas , São Paulo , Brazil .
authors.forename: Paula B T
authors.initials: PB
authors.lastname: Fettback
authors.affiliation: b Huntington Centro de Medicina Reprodutiva , São Paulo , Brazil , and.
authors.forename: Ricardo M A
authors.initials: RM
authors.lastname: Pereira
authors.affiliation: c Departments of Obstetrics and Gynecology , Urology, and Physiology, University of Michigan Medical School , Ann Arbor , MI , USA.
authors.forename: André M
authors.initials: AM
authors.lastname: Rocha
authors.affiliation: a Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Faculdade de Medicina da Universidade de São Paulo, Hospital das Clínicas , São Paulo , Brazil .
authors.forename: José Maria
authors.initials: JM
authors.lastname: Soares
authors.affiliation: c Departments of Obstetrics and Gynecology , Urology, and Physiology, University of Michigan Medical School , Ann Arbor , MI , USA.
authors.forename: Gary D
authors.initials: GD
authors.lastname: Smith
authors.affiliation: a Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Faculdade de Medicina da Universidade de São Paulo, Hospital das Clínicas , São Paulo , Brazil .
authors.forename: Edmund C
authors.initials: EC
authors.lastname: Baracat
authors.affiliation: a Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Faculdade de Medicina da Universidade de São Paulo, Hospital das Clínicas , São Paulo , Brazil .|b Huntington Centro de Medicina Reprodutiva , São Paulo , Brazil , and.
authors.forename: Paulo C
authors.initials: PC
authors.lastname: Serafini
mesh_terms: D000328:Adult; D001706:Biopsy; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D010535:Laparoscopy; D010865:Pilot Projects; D011446:Prospective Studies; D012333:RNA, Messenger / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*; D055815:Young Adult
chemical_list: D012333:RNA, Messenger
keywords: Endometriosis; gene expression; stem cells