title: Preparation and identification of an antiserum against recombinant UL31 protein of pseudorabies virus.
abstract: Pseudorabies virus (PRV) early protein UL31 is a homologue of herpes simplex virus 1 (HSV-1) UL31, which is a multifunctional protein important for HSV-1 infection. However, the precise roles of PRV UL31 in virus life cycle are still poorly understood. A relatively crucial tool for uncovering the function of UL31 is an antiserum that specifically detects UL31 in the PRV-infected cells. For this purpose, a recombinant UL31 protein consisting of N-terminal 27 aa of UL31 fused to EYFP and His-tag was expressed, purified and used for the preparation of antiserum in BALB/c mice. Our results show that Western blot analysis and immunofluorescence assay showed that this antiserum could specifically detect the purified recombinant UL31 as well as full-length UL31 in the PRV infected cells. These results demonstrate that the prepared antiserum could serve as a valuable tool for further studies of UL31 functions in PRV infection.
authors.forename: M S
authors.initials: MS
authors.lastname: Cai
authors.forename: S
authors.initials: S
authors.lastname: Jiang
authors.forename: C C
authors.initials: CC
authors.lastname: Mo
authors.forename: J L
authors.initials: JL
authors.lastname: Wang
authors.forename: J L
authors.initials: JL
authors.lastname: Huang
authors.forename: Z C
authors.initials: ZC
authors.lastname: Zeng
authors.forename: X W
authors.initials: XW
authors.lastname: Li
authors.forename: Y J
authors.initials: YJ
authors.lastname: Yang
authors.forename: C K
authors.initials: CK
authors.lastname: Chen
authors.forename: W
authors.initials: W
authors.lastname: Cui
authors.forename: M L
authors.initials: ML
authors.lastname: Li
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D057809:HEK293 Cells; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology*
chemical_list: D007106:Immune Sera; D011994:Recombinant Proteins; C000592811:UL31 protein, pseudorabies virus; D014764:Viral Proteins
keywords: pseudorabies virus; UL31 protein; recombinant protein; antiserum.