title: Immunostaining of Lymphocytes in Mouse Atherosclerotic Plaque.
abstract: Immunostaining can be defined as a method for identification of constituents in situ, e.g., proteins, using specific antigen-antibody interactions, visualized by color or fluorescence. In experimental research of atherosclerosis, immunostaining is a key method used for the characterization of the cellular composition in the atherosclerotic plaques. This includes the quantification of macrophages, smooth muscle cells, and lymphocytes-especially T lymphocytes. In this chapter we focus on basic protocols for characterization of lymphocytes and/or local T lymphocyte-related antigens in mouse atherosclerotic lesions.
authors.affiliation: Center for Molecular Medicine, Department of Medicine, Karolinska University Hospital, Karolinska Institute, SE-17176, Stockholm, Sweden.
authors.forename: Anton
authors.initials: A
authors.lastname: Gisterå
authors.affiliation: Center for Molecular Medicine, Department of Medicine, Karolinska University Hospital, Karolinska Institute, SE-17176, Stockholm, Sweden. daniel.ketelhuth@ki.se.
authors.forename: Daniel F J
authors.initials: DF
authors.lastname: Ketelhuth
mesh_terms: D000818:Animals; D001011:Aorta / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D001018:Aortic Diseases / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D050197:Atherosclerosis / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry / Q000379:methods*; D008213:Lymphocyte Activation; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D058226:Plaque, Atherosclerotic; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology*
chemical_list: D015415:Biomarkers
keywords: Atherosclerosis; Immunofluorescence; Immunohistochemistry; Immunostaining; Lymphocytes; T lymphocytes