title: Microbial community characteristics during simultaneous nitrification-denitrification process: effect of COD/TP ratio.
abstract: To evaluate the impact of chemical oxygen demand (COD)/total phosphorus (TP) ratio on microbial community characteristics during low-oxygen simultaneous nitrification and denitrification process, three anaerobic-aeration (low-oxygen) sequencing batch reactors, namely R1, R2, and R3, were performed under three different COD/TP ratios of 91.6, 40.8, and 27.6. The community structures of each reactor were analyzed via molecular biological technique. The results showed that the composition of ammonia-oxidizing bacteria (AOB) was affected, indicated by Shannon indexes of the samples from R1, R2, and R3. Nitrosomonas was identified to be the dominant AOB in all SBRs. Moreover, the copy numbers of nitrifiers were more than those of denitrifiers, and the phosphorus-accumulating organisms to glycogen-accumulating organisms ratio increased with the decrease of COD/TP ratio.
authors.affiliation: Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu Normal University, Xuzhou, 221116, China.
authors.forename: Wenlin
authors.initials: W
authors.lastname: Jia
authors.affiliation: Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.affiliation: School of Civil and Environmental Engineering, University of Technology Sydney, Broadway, NSW 2007, Australia.
authors.forename: Huu Hao
authors.initials: HH
authors.lastname: Ngo
authors.affiliation: School of Civil and Environmental Engineering, University of Technology Sydney, Broadway, NSW 2007, Australia.
authors.forename: Wenshan
authors.initials: W
authors.lastname: Guo
authors.affiliation: Environmental Research Institute, Shandong University, Jinan, 250100, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Xie
authors.affiliation: Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China. liangshuang@sdu.edu.cn.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
mesh_terms: D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D058440:Denitrification; D005068:Eutrophication; D058465:Nitrification; D009584:Nitrogen / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D014871:Water Microbiology; D018508:Water Purification
chemical_list: D010758:Phosphorus; D009584:Nitrogen; D010100:Oxygen
keywords: COD/TP ratio; Microbial community; Molecular biological technique; Simultaneous nitrification and denitrification