title: Interaction of a snake venom L-amino acid oxidase with different cell types membrane.
abstract: Snake venom l-amino acid oxidases are multifunctional enzymes that exhibited a wide range of pharmacological activities. Although it has been established that these activities are primarily caused by the H2O2 generated in the enzymatic reaction, the molecular mechanism, however, has not been fully investigated. In this work, LAAO interaction with cytoplasmic membranes using different cell types and Langmuir interfacial monolayers was evaluated. The Cerastes cerastes venom LAAO (CC-LAAO) did not exhibit cytotoxic activities against erythrocytes and peripheral blood mononuclear cells (PBMC). However, CC-LAAO caused cytotoxicity on several cancer cell lines and induced platelet aggregation in dose-dependent manner. Furthermore, the enzyme showed remarkable effect against Gram-positive and Gram-negative bacteria. These activities were inhibited on the addition of catalase or substrate analogs, suggesting that H2O2 liberation× is required for these effects. Binding studies revealed that CC-LAAO binds to the cell surface and enables the production of highly localized concentration of H2O2 in or near the binding interfaces. On another hand, the interaction of CC-LAAO with a mimetic phospholipid film was evaluated, for the first time, using a monomolecular film technique. Results indicated that phospholipid/CC-LAAO interactions are not involved in their binding to membrane and in their pharmacological activities.
authors.affiliation: Laboratoire des Venins et Biomolécules Thérapeutiques LR11IPT08, Institut Pasteur de Tunis, 13, Place Pasteur, 1002 Tunis, Tunisia; Université de Tunis el Manar, 1068 Tunis, Tunisia. Electronic address: abdelkafi_zaineb@yahoo.fr.
authors.forename: Zaineb
authors.initials: Z
authors.lastname: Abdelkafi-Koubaa
authors.affiliation: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax (ENIS), Route de Soukra, BP 1173, 3038 Sfax, Tunisia; Université de Sfax, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Aissa
authors.affiliation: Laboratoire des Venins et Biomolécules Thérapeutiques LR11IPT08, Institut Pasteur de Tunis, 13, Place Pasteur, 1002 Tunis, Tunisia; Université de Tunis el Manar, 1068 Tunis, Tunisia.
authors.forename: Maram
authors.initials: M
authors.lastname: Morjen
authors.affiliation: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax (ENIS), Route de Soukra, BP 1173, 3038 Sfax, Tunisia; Université de Sfax, Tunisia.
authors.forename: Nadia
authors.initials: N
authors.lastname: Kharrat
authors.affiliation: Laboratoire des Venins et Biomolécules Thérapeutiques LR11IPT08, Institut Pasteur de Tunis, 13, Place Pasteur, 1002 Tunis, Tunisia; Université de Tunis el Manar, 1068 Tunis, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: El Ayeb
authors.affiliation: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax (ENIS), Route de Soukra, BP 1173, 3038 Sfax, Tunisia; Université de Sfax, Tunisia.
authors.forename: Youssef
authors.initials: Y
authors.lastname: Gargouri
authors.affiliation: Laboratoire des Venins et Biomolécules Thérapeutiques LR11IPT08, Institut Pasteur de Tunis, 13, Place Pasteur, 1002 Tunis, Tunisia; Université de Tunis el Manar, 1068 Tunis, Tunisia.
authors.forename: Najet
authors.initials: N
authors.lastname: Srairi-Abid
authors.affiliation: Laboratoire des Venins et Biomolécules Thérapeutiques LR11IPT08, Institut Pasteur de Tunis, 13, Place Pasteur, 1002 Tunis, Tunisia; Université de Tunis el Manar, 1068 Tunis, Tunisia; Faculté de Médecine de Tunis, 1007 Tunis, Tunisia.
authors.forename: Naziha
authors.initials: N
authors.lastname: Marrakchi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D004249:DNA Damage; D052978:Disk Diffusion Antimicrobial Tests; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D050830:L-Amino Acid Oxidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism* / Q000633:toxicity; D010974:Platelet Aggregation / Q000187:drug effects; D011485:Protein Binding; D051381:Rats; D012910:Snake Venoms / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D012910:Snake Venoms; D050830:L-Amino Acid Oxidase
keywords: Immobilized enzyme; Membrane interaction; Phospholipid monolayers; l-Amino acid oxidase