title: Antibiofilm activity of trans-cinnamaldehyde, p-coumaric, and ferulic acids on uropathogenic Escherichia coli.
abstract: OBJECTIVE
  Biofilm on urinary catheters results in persistent infections that are resistant to antibiotics. In this study, phytochemicals were assessed as alternative antimicrobials in preventing and inactivating E. coli biofilm on urinary catheters.
  
  
  METHODS
  Biofilm prevention was tested using catheter fragments inoculated with E. coli and treated with trans- cinnamaldehyde, p-coumaric, and ferulic acids (0%, 0.1%, 0.25%, and 0.5%) for 0, 1, 3, and 5 days. Inactivation of E. coli biofilm with the same agents at concentrations of 0%, 1%, 1.25%, or 1.5% used for 0, 1, 3, or 5 days was also evaluated.
  
  
  RESULTS
  All used concentrations of trans-cinnamaldehyde prevented and effectively inactivated E. coli biofilm formed on urinary catheter fragments. p-Coumaric (0.25% and 0.5%) and ferulic acids (0.5%) had preventive action on E. coli biofilm formation in urinary catheter fragments. The number of uropathogenic E. coli cells in biofilm formed in the lumen of a urinary catheter was significantly reduced in the presence of p-coumaric and ferulic acids, but complete inactivation of the biofilm formed was not observed, as opposed to the use of trans-cinnamaldehyde.
  
  
  CONCLUSIONS
  The obtained results indicate that phytochemicals maybe an important source of antibiofilm agents that have preventive action on E. coli biofilm formation on urinary catheters.
authors.forename: Barbara
authors.initials: B
authors.lastname: Kot
authors.forename: Jolanta
authors.initials: J
authors.lastname: Wicha
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Piechota
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wolska
authors.forename: Agata
authors.initials: A
authors.lastname: Gruzewska
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D002934:Cinnamates / Q000494:pharmacology*; D003373:Coumaric Acids / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology; D006801:Humans; D050296:Microbial Viability / Q000187:drug effects; D011422:Propionates; D016459:Prosthesis-Related Infections / Q000517:prevention & control*; D062885:Urinary Catheters / Q000009:adverse effects*; D014552:Urinary Tract Infections / Q000517:prevention & control*
chemical_list: D000900:Anti-Bacterial Agents; D002934:Cinnamates; D003373:Coumaric Acids; D011422:Propionates; C004999:ferulic acid; C495469:p-coumaric acid