title: Yeast expressed ArtinM shares structure, carbohydrate recognition, and biological effects with native ArtinM.
abstract: Recent advances in glycobiology have revealed the essential role of lectins in deciphering the glycocodes at the cell surface to generate important biological signaling responses. ArtinM, a d-mannose-binding lectin isolated from the seeds of jackfruit (Artocarpus heterophyllus), is composed of 16 kDa subunits that are associated to form a homotetramer. Native ArtinM (n-ArtinM) exerts immunomodulatory and regenerative effects, but the potential pharmaceutical applicability of the lectin is highly limited by the fact that its production is expensive, laborious, and impossible to be scaled up. This led us to characterize a recombinant form of the lectin obtained by expression in Saccharomyces cerevisiae (y-ArtinM). In the present study, we demonstrated that y-ArtinM is similar to n-ArtinM in subunit arrangement, oligomerization and carbohydrate binding specificity. We showed that y-ArtinM can exert n-ArtinM biological activities such as erythrocyte agglutination, stimulation of neutrophil migration and degranulation, mast cell degranulation, and induction of interleukin-12 and interleukin-10 production by macrophages. In summary, the expression of ArtinM in yeast resulted in successful production of an active, recombinant form of ArtinM that is potentially useful for pharmaceutical application.
authors.affiliation: Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil. Electronic address: ceciliont@gmail.com.
authors.forename: Nerry Tatiana
authors.initials: NT
authors.lastname: Cecílio
authors.affiliation: Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil. Electronic address: nandafercarvalho@hotmail.com.
authors.forename: Fernanda Caroline
authors.initials: FC
authors.lastname: Carvalho
authors.affiliation: Glycosciences Laboratory, Department of Medicine, Imperial College London, London, United Kingdom. Electronic address: yan.liu2@imperial.ac.uk.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Biochemistry, Cambridge University, Cambridge, United Kingdom. Electronic address: mcm35@cam.ac.uk.
authors.forename: Martin
authors.initials: M
authors.lastname: Moncrieffe
authors.affiliation: Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil. Electronic address: patburanello@yahoo.com.br.
authors.forename: Patrícia Andressa de Almeida
authors.initials: PA
authors.lastname: Buranello
authors.affiliation: Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil. Electronic address: afernandesrp@gmail.com.
authors.forename: Andre Luiz
authors.initials: AL
authors.lastname: Zorzetto-Fernandes
authors.affiliation: Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Brazil. Electronic address: dougluche@msn.com.
authors.forename: Douglas Dalle
authors.initials: DD
authors.lastname: Luche
authors.affiliation: Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil. Electronic address: itahanna@gmail.com.
authors.forename: Ebert Seixas
authors.initials: ES
authors.lastname: Hanna
authors.affiliation: Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil. Electronic address: soaressg@hotmail.com.
authors.forename: Sandro Gomes
authors.initials: SG
authors.lastname: Soares
authors.affiliation: Glycosciences Laboratory, Department of Medicine, Imperial College London, London, United Kingdom. Electronic address: t.feizi@imperial.ac.uk.
authors.forename: Ten
authors.initials: T
authors.lastname: Feizi
authors.affiliation: Department of Biochemistry, Cambridge University, Cambridge, United Kingdom. Electronic address: njg11@mole.bio.cam.ac.uk.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Gay
authors.affiliation: Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Brazil. Electronic address: mgoldman@ffclrp.usp.br.
authors.forename: Maria Helena S
authors.initials: MH
authors.lastname: Goldman
authors.affiliation: Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil. Electronic address: mcrbarre@fmrp.usp.br.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Roque-Barreira
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D015550:Cell Degranulation / Q000187:drug effects / Q000276:immunology; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D006384:Hemagglutination; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D037241:Mannose-Binding Lectins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry; D011485:Protein Binding; D011994:Recombinant Proteins; D051195:Toll-Like Receptor 2; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
chemical_list: D002241:Carbohydrates; D016207:Cytokines; D037241:Mannose-Binding Lectins; D011134:Polysaccharides; D011994:Recombinant Proteins; D051195:Toll-Like Receptor 2
keywords: ArtinM; Immunomodulatory lectins; Recombinant proteins