title: Polyclonal Peptide Antisera.
abstract: Polyclonal antibodies are relatively easy to produce and may supplement monoclonal antibodies for some applications or even have some advantages. The choice of species for production of (peptide) antisera is based on practical considerations, including availability of immunogen (vaccine) and animals. Two major factors govern the production of antisera: the nature of adaptive immune responses, which take place over days/weeks and ethical guidelines for animal welfare. Here, simple procedures for immunization of mice, rabbits, sheep, goats, pigs, horses, and chickens are presented.
authors.affiliation: Department of Large Animal Sciences, Medicine, and Surgery, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Tina H
authors.initials: TH
authors.lastname: Pihl
authors.affiliation: Department of Quality Control, Statens Serum Institut, Copenhagen, Denmark.
authors.forename: Kristin E
authors.initials: KE
authors.lastname: Illigen
authors.affiliation: Department of Autoimmunology and Biomarkers, Statens Serum Institut, Copenhagen, Denmark. gh@ssi.dk.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Houen
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D002645:Chickens; D006736:Horses; D007106:Immune Sera / Q000276:immunology*; D007114:Immunization / Q000379:methods; D007118:Immunoassay; D051379:Mice; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology*; D011817:Rabbits; D012756:Sheep; D013552:Swine
chemical_list: D000941:Antigens; D007106:Immune Sera; D010455:Peptides
keywords: Bleeding; Coagulation; Goats; Horses and chickens; Immunization; Immunoassays; Mice; Peptide antisera; Pigs; Polyclonal antibodies; Rabbits; Serum; Sheep