title: A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica.
abstract: A PP2C homolog gene was cloned from the drought-treated cDNA library of Populus euphratica. Multiple sequence alignment analysis suggested that the gene is a potential ortholog of HAB1. The expression of this HAB1 ortholog (PeHAB1) was markedly induced by drought and moderately induced by ABA. To characterize its function in ABA signaling, we generated transgenic Arabidopsis thaliana plants overexpressing this gene. Transgenic lines exhibited reduced responses to exogenous ABA and reduced tolerance to drought compared to wide-type lines. Yeast two-hybrid analyses indicated that PeHAB1 could interact with the ABA receptor PYL4 in an ABA-independent manner. Taken together; these results indicated that PeHAB1 is a new negative regulator of ABA responses in poplar.
authors.affiliation: College of Biological Sciences and technology, Beijing Forestry University, Beijing, China; National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China.
authors.forename: Jinhuan
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Biological Sciences and technology, Beijing Forestry University, Beijing, China; National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China.
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Zhang
authors.affiliation: College of Biological Sciences and technology, Beijing Forestry University, Beijing, China; National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Biological Sciences and technology, Beijing Forestry University, Beijing, China; National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China.
authors.forename: Xinli
authors.initials: X
authors.lastname: Xia
authors.affiliation: College of Biological Sciences and technology, Beijing Forestry University, Beijing, China.
authors.forename: Weilun
authors.initials: W
authors.lastname: Yin
authors.affiliation: College of Biological Sciences and technology, Beijing Forestry University, Beijing, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Tian
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics*; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000235:genetics; D032107:Populus / Q000235:genetics*; D016415:Sequence Alignment; D015398:Signal Transduction / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins