title: Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 → 3)-linked β-D-glucan oligosaccharides.
abstract: In order to find an efficient enzymatic tool for curdlan degradation to produce (1 → 3)-linked β-D-glucan oligosaccharides, strain E4-5 (registration number JN089883, Genbank) was isolated from seaside soil. The 16S rRNA gene sequencing classified it as Cellulosimicrobium cellulans. It was the first reported microorganism that succeeded in degrading high-set heated curdlan blocks. The ferments of strain E4-5 also showed good degradation effects on laminaran and alkali-neutralized curdlan. Due to the products with less amount of glucose, it was assumed that endo-1,3-β-glucanases of strain E4-5 had a greater hydrolyzing effect than exo-1,3-β-glucanases. This indicated that strain E4-5 was a promising microorganism to hydrolyze (1 → 3)-linked β-D-glucan. Moreover, alkali-neutralization pretreatment was effective for promoting a more diversified degree of polymerization (DP) of (1 → 3)-linked β-D-glucan oligosaccharides under enzymatic hydrolysis and will pave the way for making full use of curdlan for production of glucan oligosaccharides.
authors.affiliation: Natural Products and Glyco-Biotechnology Group 1805, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Yunbin
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Natural Products and Glyco-Biotechnology Group 1805, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.
authors.forename: Likun
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Natural Products and Glyco-Biotechnology Group 1805, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China; College of Science, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Yanyu
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Natural Products and Glyco-Biotechnology Group 1805, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Li
authors.affiliation: Natural Products and Glyco-Biotechnology Group 1805, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Natural Products and Glyco-Biotechnology Group 1805, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China; Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: duyg@dicp.ac.cn.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Du
authors.affiliation: Natural Products and Glyco-Biotechnology Group 1805, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China. Electronic address: yinheng@dicp.ac.cn.
authors.forename: Heng
authors.initials: H
authors.lastname: Yin
mesh_terms: D039903:Actinobacteria / Q000201:enzymology*; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009844:Oligosaccharides / Q000378:metabolism*; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D047071:beta-Glucans; C038459:curdlan; D004693:Glucan Endo-1,3-beta-D-Glucosidase
keywords: (1→3)-Linked β-d-glucan oligosaccharides; Alkali-neutralization treatment; Cellulosimicrobium cellulans; Curdlan; Enzymatic degradation; High-set gel