title: Methods for TALEN-Mediated Genomic Manipulations in Drosophila.
abstract: TALEN (transcription activator-like effector nuclease) is a powerful tool for gene disruption and other genomic modifications. In the past 3 years or so, it has attracted eyes from every corner of the biological world, due to its characteristics of simplicity, high efficiency, low toxicity, and applicability across almost all species. In our lab, we first reported the TALEN-mediated gene disruption in Drosophila, and recently employed this technique in precisely modifying the Drosophila genome, such as in vivo tagging and gene correction. Here, we describe in detail the protocols and experiences in TALEN-mediated genomic modifications to share with the Drosophilists all over the world.
authors.affiliation: State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Datun Road 15, Beijing, 100101, China.|Guangzhou Hoffmann Institute of Immunology, School of Basic Sciences, Guangzhou Medical University, Dongfengxi Road 195, Guangzhou, 510182, China.
authors.forename: Jiyong
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Datun Road 15, Beijing, 100101, China.
authors.forename: Yawen
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Datun Road 15, Beijing, 100101, China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Datun Road 15, Beijing, 100101, China.
authors.forename: Yixu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Datun Road 15, Beijing, 100101, China. rjiao@sun5.ibp.ac.cn.|Guangzhou Hoffmann Institute of Immunology, School of Basic Sciences, Guangzhou Medical University, Dongfengxi Road 195, Guangzhou, 510182, China. rjiao@sun5.ibp.ac.cn.
authors.forename: Renjie
authors.initials: R
authors.lastname: Jiao
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics*; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D049750:Genome, Insect / Q000235:genetics; D023281:Genomics; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D015534:Trans-Activators; D004720:Endonucleases
keywords: Drosophila; Genomic modifications; HR; NHEJ; TALEN