title: A two-hour procedure for determining the susceptibility of enterococci and staphylococci to antibiotics by a colourimetric method.
abstract: BACKGROUND
  Rapid determination of the antibiotic susceptibility test in bacteria remains a challenge for Clinical Microbiology laboratories.
  
  
  METHODS
  An improvement in the colorimetric antimicrobial susceptibility testing performed with resazurin in enterococci and staphylococci has been carried out. The design of method was performed using two collection strains, which have a known susceptibility. This procedure was then validated against standard commercial methods on 15 strains of staphylococci and 15 strains of enterococci from patients.
  
  
  RESULTS
  The essential agreement between the colorimetric method and commercial methods (E-test, MicroScan and VITEK2) was 100%.
  
  
  CONCLUSIONS
  Resazurin allows us to obtain a reliable antibiotic susceptibility test in staphylococci and enterococci in less than two hours.
authors.affiliation: Gabriel Alberto March Rosselló, Servicio de Microbiología e Inmunología, Hospital Clínico Universitario de Valladolid, Spain. gmr810@hotmail.com.
authors.forename: Gabriel Alberto
authors.initials: GA
authors.lastname: March-Rosselló
authors.forename: María Purificación
authors.initials: MP
authors.lastname: Gutiérrez-Rodríguez
authors.forename: María
authors.initials: M
authors.lastname: Simarro-Grande
authors.forename: Antonio
authors.initials: A
authors.lastname: Orduña-Domingo
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Bratos-Pérez
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003124:Colorimetry; D016983:Enterococcus / Q000187:drug effects*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D007202:Indicators and Reagents; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010078:Oxazines; D015203:Reproducibility of Results; D013203:Staphylococcal Infections / Q000382:microbiology; D013210:Staphylococcus / Q000187:drug effects*; D014966:Xanthenes
chemical_list: D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin