title: Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages.
abstract: Macrophages are the most specialized phagocytic cells, and acquire specific phenotypes and functions in response to a variety of external triggers. Culture of bone marrow-derived or peritoneal macrophages from mice represents an exceptionally powerful technique to investigate macrophage phenotypes and functions in response to specific stimuli, resembling as much as possible the conditions observed in various pathophysiological settings. This chapter outlines protocols used to isolate and culture murine bone marrow-derived and peritoneal macrophages. Furthermore, we describe how these macrophages can be "polarized" to obtain specific macrophage subsets with special relevance to atherosclerosis.
authors.affiliation: Division of Medicine, Centre for Clinical Pharmacology, University College London, 5 University Street, London, WC1 E6JF, UK. i.torra@ucl.ac.uk.
authors.forename: Inés
authors.initials: I
authors.lastname: Pineda-Torra
authors.affiliation: Division of Medicine, Centre for Clinical Pharmacology, University College London, 5 University Street, London, WC1 E6JF, UK.
authors.forename: Matthew
authors.initials: M
authors.lastname: Gage
authors.affiliation: Walter Brendel Center for Experimental Medicine, Ludwig-Maximilians-Universität, Marchioninistr. 27, 81377, Munich, Germany.
authors.forename: Alba
authors.initials: A
authors.lastname: de Juan
authors.affiliation: Division of Medicine, Centre for Clinical Pharmacology, University College London, 5 University Street, London, WC1 E6JF, UK. o.pello@imperial.ac.uk.|Department of Hematology, John Goldman Centre for Cellular Therapy, Hammersmith Hospital, 150 Du-Cane Road, London, W12 0HS, UK. o.pello@imperial.ac.uk.
authors.forename: Oscar M
authors.initials: OM
authors.lastname: Pello
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D010641:Phenotype; D061251:Primary Cell Culture
chemical_list: D003470:Culture Media; D016207:Cytokines
keywords: Atherosclerosis; Bone marrow-derived macrophages; Macrophage polarization; Peritoneal macrophages