title: Evaluating Osteogenic Potential of Ligamentum Flavum Cells Cultivated in Photoresponsive Hydrogel that Incorporates Bone Morphogenetic Protein-2 for Spinal Fusion.
abstract: Regenerative medicine is increasingly important in clinical practice. Ligamentum flava (LF) are typically removed during spine-related surgeries. LF may be a source of cells for spinal fusion that is conducted using tissue engineering techniques. In this investigation, LF cells of rabbits were isolated and then characterized by flow cytometry, morphological observation, and immunofluorescence staining. The LF cells were also cultivated in polyethylene (glycol) diacrylate (PEGDA) hydrogels that incorporated bone morphogenetic protein-2 (BMP-2) growth factor, to evaluate their proliferation and secretion of ECM and differentiation in vitro. The experimental results thus obtained that the proliferation, ECM secretion, and differentiation of the PEGDA-BMP-2 group exceeded those of the PEGDA group during the period of cultivation. The mineralization and histological staining results differed similarly. A nude mice model was utilized to prove that LF cells on hydrogels could undergo osteogenic differentiation in vivo. These experimental results also revealed that the PEGDA-BMP-2 group had better osteogenic effects than the PEGDA group following a 12 weeks after transplantation. According to all of these experimental results, LF cells are a source of cells for spinal fusion and PEGDA-BMP-2 hydrogel is a candidate biomaterial for spinal fusion by tissue engineering.
authors.affiliation: Bone and Joint Research Center, Department of Orthopedics and Traumatology, Taipei Medical University Hospital, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan. kiwi8502017@gmail.com.|Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 106, Taiwan. kiwi8502017@gmail.com.
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Chiang
authors.affiliation: Bone and Joint Research Center, Department of Orthopedics and Traumatology, Taipei Medical University Hospital, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan. itispay@gmail.com.
authors.forename: Wei-Chuan
authors.initials: WC
authors.lastname: Chen
authors.affiliation: Department of Life Science, College of Science and Engineering, Fu Jen Catholic University, New Taipei City 242, Taiwan. 079336@mail.fju.edu.tw.
authors.forename: Hsia-Wei
authors.initials: HW
authors.lastname: Liu
authors.affiliation: Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Keelung 204, Taiwan. icwang@adm.cgmh.org.tw.|College of Medicine, Chang Gung University, Taoyuan 333, Taiwan. icwang@adm.cgmh.org.tw.
authors.forename: I-Chun
authors.initials: IC
authors.lastname: Wang
authors.affiliation: Bone and Joint Research Center, Department of Orthopedics and Traumatology, Taipei Medical University Hospital, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan. kiwi8502017@gmail.com.|Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110, Taiwan. kiwi8502017@gmail.com.
authors.forename: Chih-Hwa
authors.initials: CH
authors.lastname: Chen
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D017843:Ligamentum Flavum / Q000166:cytology*; D008027:Light; D008297:Male; D008819:Mice, Nude; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D011817:Rabbits; D013123:Spinal Fusion
chemical_list: D055396:Bone Morphogenetic Protein 2; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: BMP-2; Ligamentum flavum; PEGDA hydrogels; spinal fusion; tissue engineering