title: Complete genome sequence of the chromate-reducing bacterium Thermoanaerobacter thermohydrosulfuricus strain BSB-33.
abstract: Thermoanaerobacter thermohydrosulfuricus BSB-33 is a thermophilic gram positive obligate anaerobe isolated from a hot spring in West Bengal, India. Unlike other T. thermohydrosulfuricus strains, BSB-33 is able to anaerobically reduce Fe(III) and Cr(VI) optimally at 60 °C. BSB-33 is the first Cr(VI) reducing T. thermohydrosulfuricus genome sequenced and of particular interest for bioremediation of environmental chromium contaminations. Here we discuss features of T. thermohydrosulfuricus BSB-33 and the unique genetic elements that may account for the peculiar metal reducing properties of this organism. The T. thermohydrosulfuricus BSB-33 genome comprises 2597606 bp encoding 2581 protein genes, 12 rRNA, 193 pseudogenes and has a G + C content of 34.20 %. Putative chromate reductases were identified by comparative analyses with other Thermoanaerobacter and chromate-reducing bacteria.
authors.affiliation: Life Sciences Division, Lawrence Berkeley National Laboratory, Building 84, Mail Stop 84-171, 1 Cyclotron Road, Berkeley, CA 94720 USA.
authors.forename: Pamela
authors.initials: P
authors.lastname: Bhattacharya
authors.affiliation: Life Sciences Division, Lawrence Berkeley National Laboratory, Building 84, Mail Stop 84-171, 1 Cyclotron Road, Berkeley, CA 94720 USA.
authors.forename: Adam
authors.initials: A
authors.lastname: Barnebey
authors.affiliation: Life Sciences Division, Lawrence Berkeley National Laboratory, Building 84, Mail Stop 84-171, 1 Cyclotron Road, Berkeley, CA 94720 USA.
authors.forename: Marcin
authors.initials: M
authors.lastname: Zemla
authors.affiliation: Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM USA.
authors.forename: Lynne
authors.initials: L
authors.lastname: Goodwin
authors.affiliation: Life Sciences Division, Lawrence Berkeley National Laboratory, Building 84, Mail Stop 84-171, 1 Cyclotron Road, Berkeley, CA 94720 USA.
authors.forename: Manfred
authors.initials: M
authors.lastname: Auer
authors.affiliation: Life Sciences Division, Lawrence Berkeley National Laboratory, Building 84, Mail Stop 84-171, 1 Cyclotron Road, Berkeley, CA 94720 USA.
authors.forename: Steven M
authors.initials: SM
authors.lastname: Yannone
keywords: Anaerobic; Bioremediation; Chromate; Chromium; Gram-positive; Metal; Reducing; Thermoanaerobacter; Thermophilic