title: p38 MAPK regulates PKAα and CUB-serine protease in Amphibalanus amphitrite cyprids.
abstract: The MKK3-p38 MAPK pathway has been reported to mediate larval settlement in Amphibalanus (=Balanus) amphitrite. To clarify the underlying molecular mechanism, we applied label-free proteomics to analyze changes in the proteome of cyprids treated with a p38 MAPK inhibitor. The results showed that the expression levels of 80 proteins were significantly modified (p < 0.05). These differentially expressed proteins were assigned to 15 functional groups according to the KOG database and 9 pathways were significantly enriched. Further analysis revealed that p38 MAPK might regulate the energy supply and metamorphosis. Two potential regulatory proteins, CUB-serine protease and PKAα, were both down-regulated in expression. CUB-serine protease localized to postaxial seta 2 and 3, as well as the 4 subterminal sensilla in the antennule. Importantly, it was co-localized with the neuron transmitter serotonin in the sections, suggesting that the CUB-serine protease was present in the neural system. PKAα was highly expressed during the cyprid and juvenile stages, and it was co-localized with phospho-p38 MAPK (pp38 MAPK) to the cement gland, suggesting that PKAα might have some functions in cement glands. Overall, p38 MAPK might regulate multiple functions in A. amphitrite cyprids, including the energy supply, metamorphosis, neural system and cement glands.
authors.affiliation: Environmental Science Programs and Division of Life Science, School of Science, the Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, P. R. China.
authors.forename: Gen
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Sanya Institute of Deep-sea Science and Engineering, Chinese Academy of Science, No. 62, Fenghuang Road, Sanya, Hainan, P. R. China, 572000.
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: He
authors.affiliation: Environmental Science Programs and Division of Life Science, School of Science, the Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, P. R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Him Wong
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Science, Shenzhen University, Shenzhen, P. R. China, 518060.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Science, Shenzhen University, Shenzhen, P. R. China, 518060.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Environmental Science Programs and Division of Life Science, School of Science, the Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, P. R. China.
authors.forename: Pei-Yuan
authors.initials: PY
authors.lastname: Qian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020134:Catalytic Domain; D017124:Conserved Sequence; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D025461:Feedback, Physiological; D007093:Imidazoles / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000201:enzymology; D020935:MAP Kinase Signaling System; D008969:Molecular Sequence Data; D009928:Organ Specificity; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D021381:Protein Transport; D020543:Proteome / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000378:metabolism; D001468:Thoracica / Q000187:drug effects / Q000201:enzymology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000502:physiology*
chemical_list: D007093:Imidazoles; D047428:Protein Kinase Inhibitors; D020543:Proteome; D011725:Pyridines; D017868:Cyclic AMP-Dependent Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; C445579:CUB-serine protease, Panulirus argus; D012697:Serine Endopeptidases; C093642:SB 203580