title: Global analysis of lysine acetylation in strawberry leaves.
abstract: Protein lysine acetylation is a reversible and dynamic post-translational modification. It plays an important role in regulating diverse cellular processes including chromatin dynamic, metabolic pathways, and transcription in both prokaryotes and eukaryotes. Although studies of lysine acetylome in plants have been reported, the throughput was not high enough, hindering the deep understanding of lysine acetylation in plant physiology and pathology. In this study, taking advantages of anti-acetyllysine-based enrichment and high-sensitive-mass spectrometer, we applied an integrated proteomic approach to comprehensively investigate lysine acetylome in strawberry. In total, we identified 1392 acetylation sites in 684 proteins, representing the largest dataset of acetylome in plants to date. To reveal the functional impacts of lysine acetylation in strawberry, intensive bioinformatic analysis was performed. The results significantly expanded our current understanding of plant acetylome and demonstrated that lysine acetylation is involved in multiple cellular metabolism and cellular processes. More interestingly, nearly 50% of all acetylated proteins identified in this work were localized in chloroplast and the vital role of lysine acetylation in photosynthesis was also revealed. Taken together, this study not only established the most extensive lysine acetylome in plants to date, but also systematically suggests the significant and unique roles of lysine acetylation in plants.
authors.affiliation: Institute of Biology, Hangzhou Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Xianping
authors.initials: X
authors.lastname: Fang
authors.affiliation: Institute of Biology, Hangzhou Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Wenyue
authors.initials: W
authors.lastname: Chen
authors.affiliation: Experiment Center, Hangzhou Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Biology, Hangzhou Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Songlin
authors.initials: S
authors.lastname: Ruan
authors.affiliation: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Hengmu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Chengqi
authors.initials: C
authors.lastname: Yan
authors.affiliation: Research and Development Center of Flower, Zhejiang Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Jin
authors.affiliation: Jingjie PTM Biolabs Hangzhou, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Cao
authors.affiliation: Jingjie PTM Biolabs Hangzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Institute of Biology, Hangzhou Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Huasheng
authors.initials: H
authors.lastname: Ma
authors.affiliation: Institute for Advanced Study of Translational Medicine, Tongji University Shanghai, China.
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Cheng
keywords: acetylome; bioinformatics; lysine acetylation; photosynthesis; proteomics; strawberry