title: Delayed administration of recombinant human erythropoietin reduces apoptosis and inflammation and promotes myelin repair and functional recovery following spinal cord compressive injury in rats.
abstract: A previous study showed that a 1-h delay in treatment of thoracic spinal cord injury (SCI) with recombinant human erythropoietin (rhEPO) lacked neuroprotective efficacy. The aim of the present study was to reassess delayed administration of different doses of rhEPO on acute spinal cord compressive injury in rats.
  
  
  
  The experiment was divided into first and second stages, which SCI rats were observed for 4 and 28 days, respectively. All rats were randomly divided into four groups at both stages: control group, and rhEPO-3,000U (Unit), rhEPO-4,000U and rhEPO-5,000U groups. SCI rats received rhEPO treatment at different time points. The primary indicators were locomotor recovery, histopathology, apoptotic index, inflammatory index, ultrastructural scoring system and volume of areas of demyelination.
  
  
  
  The most significant locomotor functional and histopathological improvements and the best myelin protection were observed after administration of 5,000 U/kg rhEPO. rhEPO at 3,000, 4,000 and 5,000 U/kg showed similar ultrastructural neuroprotection, as well as similar inhibition of apoptosis and regulation of inflammation.
  
  
  
  Delayed administration of rhEPO can reduce apoptosis and inflammation, and promote myelin repair and functional recovery following spinal cord compressive injury in rats.
authors.affiliation: Department of Orthopedics, Southern Medical University, Zhujiang Hospital, Guangzhou, Guangdong, China.|Department of Orthopedics, Hezhou city pepole's hospital, Hezhou, Guangxi, China.
authors.forename: Liuzhu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, Southern Medical University, Zhujiang Hospital, Guangzhou, Guangdong, China.
authors.forename: Xinping
authors.initials: X
authors.lastname: Yan
authors.affiliation: Department of Orthopedics, Southern Medical University, Zhujiang Hospital, Guangzhou, Guangdong, China.
authors.forename: Zunying
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, Southern Medical University, Zhujiang Hospital, Guangzhou, Guangdong, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tan
authors.affiliation: Department of Orthopedics, Southern Medical University, Zhujiang Hospital, Guangzhou, Guangdong, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, Southern Medical University, Zhujiang Hospital, Guangzhou, Guangdong, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001522:Behavior, Animal / Q000187:drug effects; D004195:Disease Models, Animal; D004921:Erythropoietin / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy*; D008297:Male; D009043:Motor Activity / Q000187:drug effects*; D009186:Myelin Sheath / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins; D020127:Recovery of Function / Q000187:drug effects*; D013117:Spinal Cord Compression / Q000150:complications*; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology; D013997:Time Factors
chemical_list: C554270:EPO protein, human; D011994:Recombinant Proteins; D004921:Erythropoietin
keywords: Erythropoietin; delayed treatment; functional recovery; myelin; rats; spinal cord injury