title: Antibacterial activity and in vitro evaluation of the biocompatibility of chitosan-based polysaccharide/polyester membranes.
abstract: The antibacterial activity and biocompatibility of membranes of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and chitosan (CS) (PHBV)/CS) were evaluated in this study. Maleic anhydride (MA)-grafted polyhydroxyalkanoate (PHBV-g-MA) was evaluated as an alternative to PHBV. Mouse tail skin fibroblasts (FBs) were seeded on two series of these films to assess cytocompatibility. Collagen and cell proliferation analyses indicated that PHBV, PHBV-g-MA and their composite membranes were biocompatible with respect to FB proliferation. However, FB proliferation, collagen production and the percentage of normal cells growing on PHBV/CS membranes were greater than those for PHBV-g-MA/CS membranes. Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications. Moreover, CS-based polysaccharide enhanced the Escherichia coli (BCRC 10239) antibacterial activity of the membranes. Membranes of PHBV-g-MA or PHBV containing CS-based polysaccharide had better antibacterial activity.
authors.affiliation: Department of Chemical and Biochemical Engineering, Kao Yuan University, Kaohsiung County 82101, Taiwan, ROC. Electronic address: t50008@cc.kyu.edu.tw.
authors.forename: Chin-San
authors.initials: CS
authors.lastname: Wu
authors.affiliation: Graduate Institute of Medical Science, College of Health Sciences, Chang Jung Christian University, Tainan 71101, Taiwan, ROC; Innovative Research Center of Medicine, College of Health Sciences, Chang Jung Christian University, Tainan 71101, Taiwan, ROC.
authors.forename: Yi-Chiang
authors.initials: YC
authors.lastname: Hsu
authors.affiliation: Department of Chemical and Biochemical Engineering, Kao Yuan University, Kaohsiung County 82101, Taiwan, ROC.
authors.forename: Hsin-Tzu
authors.initials: HT
authors.lastname: Liao
authors.affiliation: Department of Chemical and Biochemical Engineering, Kao Yuan University, Kaohsiung County 82101, Taiwan, ROC.
authors.forename: Yu-Xuan
authors.initials: YX
authors.lastname: Cai
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000096:biosynthesis; D004926:Escherichia coli / Q000187:drug effects; D008299:Maleic Anhydrides / Q000737:chemistry; D008422:Materials Testing; D055595:Mechanical Phenomena; D008567:Membranes, Artificial; D051379:Mice; D011091:Polyesters / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D000094122:Polyhydroxybutyrates
chemical_list: D000900:Anti-Bacterial Agents; D048271:Chitosan; D003094:Collagen; D008299:Maleic Anhydrides; D008567:Membranes, Artificial; D011091:Polyesters; D011134:Polysaccharides; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates
keywords: Antibacterial activity; Biocompatibility; Chitosan; Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)