title: Higher expression of IL-12Rβ2 is associated with lower risk of relapse in relapsing-remitting multiple sclerosis patients on interferon-β1b therapy during 3-year follow-up.
abstract: Cytokines produced by helper T (Th)1 cells, Th17 and regulatory T cells (Treg) are involved in multiple sclerosis (MS) immunopathogenesis. Interferon (IFN)-β alters the numerous genes' expression, but how this alteration affects the treatment response is still elusive. We assessed relative gene expression of nineteen Th1/Th17/Treg-associated mediators in peripheral blood mononuclear cells and plasma levels of GM-CSF, IL-17A and IL-17F, in relapsing-remitting MS (RRMS) patients before IFN-β1b treatment initiation and at 6, 12, 24 and 36 months of therapy. All mRNA levels changed significantly during the IFN-β1b therapy. Higher IL-12Rβ2 mRNA levels were associated with lower risk of relapse. Despite recent reports regarding role of GM-CSF in MS, our study failed to demonstrate its significance as therapy response biomarker, both on the mRNA and protein level.
authors.affiliation: Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, Serbia. Electronic address: emina.milosevic@mfub.bg.ac.rs.
authors.forename: Emina
authors.initials: E
authors.lastname: Milosevic
authors.affiliation: Neurology Clinic, Clinical Centre of Serbia, School of Medicine, University of Belgrade, Dr Subotica 6, 11000 Belgrade, Serbia. Electronic address: irdujmbas@eunet.rs.
authors.forename: Irena
authors.initials: I
authors.lastname: Dujmovic
authors.affiliation: Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, Serbia. Electronic address: milosmarkovic@med.bg.ac.rs.
authors.forename: Milos
authors.initials: M
authors.lastname: Markovic
authors.affiliation: Neurology Clinic, Clinical Centre of Serbia, School of Medicine, University of Belgrade, Dr Subotica 6, 11000 Belgrade, Serbia. Electronic address: sharlotam@gmail.com.
authors.forename: Sarlota
authors.initials: S
authors.lastname: Mesaros
authors.affiliation: School of Electrical Engineering, University of Belgrade, Bulevar kralja Aleksandra 73, 11000 Belgrade, Serbia. Electronic address: g.rakocevic@gmail.com.
authors.forename: Goran
authors.initials: G
authors.lastname: Rakocevic
authors.affiliation: Neurology Clinic, Clinical Centre of Serbia, School of Medicine, University of Belgrade, Dr Subotica 6, 11000 Belgrade, Serbia. Electronic address: jelena60@eunet.rs.
authors.forename: Jelena
authors.initials: J
authors.lastname: Drulovic
authors.affiliation: Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, Serbia. Electronic address: mostarica@med.bg.ac.rs.
authors.forename: Marija
authors.initials: M
authors.lastname: Mostarica Stojkovic
authors.affiliation: Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, Serbia. Electronic address: dpopadic@med.bg.ac.rs.
authors.forename: Dusan
authors.initials: D
authors.lastname: Popadic
mesh_terms: D000328:Adult; D016000:Cluster Analysis; D015331:Cohort Studies; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004185:Disability Evaluation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007155:Immunologic Factors / Q000627:therapeutic use*; D016899:Interferon-beta / Q000627:therapeutic use*; D008297:Male; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000188:drug therapy* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D053707:Receptors, Interleukin-12 / Q000235:genetics* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D016207:Cytokines; C508439:IL12RB2 protein, human; D007155:Immunologic Factors; D012333:RNA, Messenger; D053707:Receptors, Interleukin-12; D016899:Interferon-beta
keywords: Gene expression; Granulocyte-macrophage colony-stimulating factor; Interferon-β; Interleukin-12 receptor β2 subunit; Multiple sclerosis; Therapy response