title: In Vivo ¹⁸F-FDG-PET Imaging in Mouse Atherosclerosis.
abstract: Positron emission tomography (PET) is an important technique in cardiovascular research. Vascular inflammation detected by fluorodeoxyglucose (FDG)-PET has been shown to predict cardiovascular (CV) events independent of traditional risk factors and is also highly associated with overall burden of atherosclerosis. The use of PET imaging in mouse models of atherosclerosis is challenged by the reduced size of the scanned organs. However, the last generation of dedicated PET scanners has an improved spatial resolution (<1 mm) and increased sensitivity allowing those studies to be performed. Here, we describe a procedure to perform FDG-PET experiments in atherosclerosis mouse models, the required equipment for animal handling and imaging, and the tools and procedures for image analysis and validation of the results.
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Mateo
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain.|Universidad Complutense de Madrid and CIBER de Enfermedades Respiratorias (CIBERES), Madrid, Spain.
authors.forename: Izaskun
authors.initials: I
authors.lastname: Bilbao
authors.affiliation: Departamento de Bioingeniería & Ingeniería Aeroespacial, Universidad Carlos III Madrid, Madrid, Spain.|Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Vaquero
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain.|Departamento de Bioingeniería & Ingeniería Aeroespacial, Universidad Carlos III Madrid, Madrid, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Ruiz-Cabello
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain. samuel.espana@cnic.es.
authors.forename: Samuel
authors.initials: S
authors.lastname: España
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000000981:diagnostic imaging* / Q000235:genetics; D004195:Disease Models, Animal; D004867:Equipment Design; D019788:Fluorodeoxyglucose F18; D020022:Genetic Predisposition to Disease; D007090:Image Interpretation, Computer-Assisted; D008822:Mice, Transgenic; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D064847:Multimodal Imaging; D049268:Positron-Emission Tomography / Q000295:instrumentation; D011237:Predictive Value of Tests; D019275:Radiopharmaceuticals; D015203:Reproducibility of Results; D014057:Tomography, X-Ray Computed
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18
keywords: Atherosclerosis; Computed tomography (CT); Fluorodeoxyglucose (FDG); Positron emission tomography (PET); Small animal imaging; Vascular imaging