title: Analyses of Twelve New Whole Genome Sequences of Cassava Brown Streak Viruses and Ugandan Cassava Brown Streak Viruses from East Africa: Diversity, Supercomputing and Evidence for Further Speciation.
abstract: Cassava brown streak disease is caused by two devastating viruses, Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) which are frequently found infecting cassava, one of sub-Saharan Africa's most important staple food crops. Each year these viruses cause losses of up to $100 million USD and can leave entire families without their primary food source, for an entire year. Twelve new whole genomes, including seven of CBSV and five of UCBSV were uncovered in this research, doubling the genomic sequences available in the public domain for these viruses. These new sequences disprove the assumption that the viruses are limited by agro-ecological zones, show that current diagnostic primers are insufficient to provide confident diagnosis of these viruses and give rise to the possibility that there may be as many as four distinct species of virus. Utilizing NGS sequencing technologies and proper phylogenetic practices will rapidly increase the solution to sustainable cassava production.
authors.affiliation: Mikocheni Agricultural Research Institute, Sam Nujoma Road, Box 6226, Dar es Salaam, Tanzania.
authors.forename: Joseph
authors.initials: J
authors.lastname: Ndunguru
authors.affiliation: Mikocheni Agricultural Research Institute, Sam Nujoma Road, Box 6226, Dar es Salaam, Tanzania.
authors.forename: Peter
authors.initials: P
authors.lastname: Sseruwagi
authors.affiliation: Mikocheni Agricultural Research Institute, Sam Nujoma Road, Box 6226, Dar es Salaam, Tanzania.
authors.forename: Fred
authors.initials: F
authors.lastname: Tairo
authors.affiliation: Biosciences eastern and central Africa, The International Livestock Research Institute in Nairobi, Kenya (the BecA-ILRI Hub), P.O. Box 30709, Nairobi 00100, Kenya.
authors.forename: Francesca
authors.initials: F
authors.lastname: Stomeo
authors.affiliation: Biosciences eastern and central Africa, The International Livestock Research Institute in Nairobi, Kenya (the BecA-ILRI Hub), P.O. Box 30709, Nairobi 00100, Kenya.
authors.forename: Solomon
authors.initials: S
authors.lastname: Maina
authors.affiliation: Biosciences eastern and central Africa, The International Livestock Research Institute in Nairobi, Kenya (the BecA-ILRI Hub), P.O. Box 30709, Nairobi 00100, Kenya.
authors.forename: Appolinaire
authors.initials: A
authors.lastname: Djikeng
authors.forename: Appolinaire
authors.initials: A
authors.lastname: Djinkeng
authors.affiliation: Crop Protection Branch, Department of Agriculture and Food Western Australia, Bentley Delivery Centre, Perth 6983, Western Australia, Australia.
authors.forename: Monica
authors.initials: M
authors.lastname: Kehoe
authors.affiliation: The University of Western Australia, ARC Centre of Excellence in Plant Energy Biology and School of Chemistry and Biochemistry, Crawley, Perth 6009, Western Australia, Australia.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Boykin
mesh_terms: D000351:Africa, Eastern; D016679:Genome, Viral; D002365:Manihot / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D019176:Potyviridae / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D014454:Uganda
chemical_list: D012367:RNA, Viral