title: A fluorescent probe for specific detection of cysteine in the lipid dense region of cells.
abstract: A new cysteine (Cys) specific chemodosimetric reagent () is used in imaging of endogenous Cys localized in the lipid dense region of the live Hct116 cells and the release of Cys within HepG2 cells from a drug following a biochemical transformation. A silica surface, modified with , could be used for quantitative estimation of Cys present in aqueous solution (pH 7.2) and in a human blood plasma (HBP).
authors.affiliation: Organic Chemistry Division, CSIR-National Chemical Laboratory, Pune-411008, India. a.das@ncl.res.in.
authors.forename: Firoj
authors.initials: F
authors.lastname: Ali
authors.affiliation: Organic Chemistry Division, CSIR-National Chemical Laboratory, Pune-411008, India. a.das@ncl.res.in.
authors.forename: Anila
authors.initials: A
authors.lastname: H A
authors.affiliation: Chromatin and Disease Biology Lab, National Centre for Cell Science, Pune 411007, India. samit@nccs.res.in.
authors.forename: Nandaraj
authors.initials: N
authors.lastname: Taye
authors.affiliation: Center for Materials Characterization, CSIR-National Chemical Laboratory, Pune-411008, India.
authors.forename: Rajesh G
authors.initials: RG
authors.lastname: Gonnade
authors.affiliation: Chromatin and Disease Biology Lab, National Centre for Cell Science, Pune 411007, India. samit@nccs.res.in.
authors.forename: Samit
authors.initials: S
authors.lastname: Chattopadhyay
authors.affiliation: Organic Chemistry Division, CSIR-National Chemical Laboratory, Pune-411008, India. a.das@ncl.res.in.
authors.forename: Amitava
authors.initials: A
authors.lastname: Das
mesh_terms: D000111:Acetylcysteine / Q000378:metabolism; D000179:Acrylates / Q000138:chemical synthesis / Q000737:chemistry*; D000581:Amidohydrolases / Q000378:metabolism; D001896:Boron Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D003124:Colorimetry; D003545:Cysteine / Q000032:analysis* / Q000097:blood; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D045325:HCT116 Cells; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics
chemical_list: D000179:Acrylates; D001896:Boron Compounds; D005456:Fluorescent Dyes; D000581:Amidohydrolases; C019672:aminoacylase I; D003545:Cysteine; D000111:Acetylcysteine