title: In cellulo protein labelling with Ru-conjugate for luminescence imaging and bioorthogonal photocatalysis.
abstract: Labelling of proteins with a luminescent ruthenium complex enables the direct visualization and photocatalytic reduction of aryl azide in live cells. The confinement of catalysis to the labeled proteins was visualized using an azide-based immolative linker releasing a precipitating dye.
authors.affiliation: University of Geneva, School of Chemistry and Biochemsitry, NCCR Chemical Biology, 30 quai Ernest Ansermet, Geneva, Switzerland. nicolas.winssinger@unige.ch.
authors.forename: Kalyan K
authors.initials: KK
authors.lastname: Sadhu
authors.forename: E
authors.initials: E
authors.lastname: Lindberg
authors.forename: N
authors.initials: N
authors.lastname: Winssinger
mesh_terms: D001386:Azides / Q000737:chemistry*; D002384:Catalysis; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D049449:Luminescence; D061986:MCF-7 Cells; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D055668:Photochemical Processes; D011960:Receptors, Estrogen / Q000737:chemistry* / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry*
chemical_list: D001386:Azides; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D011960:Receptors, Estrogen; D012428:Ruthenium; D066246:ErbB Receptors