title: Genome Editing Using Mammalian Haploid Cells.
abstract: Haploid cells are useful for studying gene functions because disruption of a single allele can cause loss-of-function phenotypes. Recent success in generating haploid embryonic stem cells (ESCs) in mice, rats, and monkeys provides a new platform for simple genetic manipulation of the mammalian genome. Use of haploid ESCs enhances the genome-editing potential of the CRISPR/Cas system. For example, CRISPR/Cas was used in haploid ESCs to generate multiple knockouts and large deletions at high efficiency. In addition, genome-wide screening is facilitated by haploid cell lines containing gene knockout libraries.
authors.affiliation: Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi, Gunma 371-8512, Japan. horii@gunma-u.ac.jp.
authors.forename: Takuro
authors.initials: T
authors.lastname: Horii
authors.affiliation: Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi, Gunma 371-8512, Japan. hatada@gunma-u.ac.jp.
authors.forename: Izuho
authors.initials: I
authors.lastname: Hatada
mesh_terms: D000818:Animals; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000378:metabolism; D016678:Genome; D006238:Haploidy; D006801:Humans; D008322:Mammals / Q000235:genetics*; D017393:RNA Editing / Q000235:genetics*
keywords: CRISPR/Cas; embryonic stem cell; haploid