title: Age will affect the Growth and Mineralization Ability of the Rat Osteoblast.
abstract: BACKGROUND
  Primary culture of rat osteoblast cells serves as a model in in vitro research of bone metabolism. But most of studies about osteoblast use fetal or newborn rats for cell isolation, including researches about the post-menopausal osteoporosis. This will affect the authenticity and reliability of the results. These differences have been a concern in recent years. So far, little research has focused on adult female rat osteoblasts.
  
  
  METHODS
  In this study, a systematic study was done regarding the proliferation, differentiation, and mineralization of adult female rat osteoblasts.
  
  
  RESULTS
  The study showed that the age of the host may affect the growth and the mineralization ability of osteoblast.
  
  
  CONCLUSIONS
  The result of the study suggests that in vitro studies related to osteoblasts should select the right host and appropriate observation points according to the cell growth characteristics in order to ensure the consistency of the actual changes.
authors.forename: Meng
authors.initials: M
authors.lastname: Cheng
authors.forename: Jing
authors.initials: J
authors.lastname: Zhuang
authors.forename: Ting Ting
authors.initials: TT
authors.lastname: Li
authors.forename: Wen Juan
authors.initials: WJ
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Liang Zhi
authors.initials: LZ
authors.lastname: Xu
mesh_terms: D000367:Age Factors; D000375:Aging / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D010006:Osteoblasts / Q000378:metabolism*; D015675:Osteocalcin / Q000378:metabolism; D061251:Primary Cell Culture; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000166:cytology / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D015675:Osteocalcin; D000469:Alkaline Phosphatase