title: Non-severe thermochemical hydrolysis of stover from white corn and sequential enzymatic saccharification and fermentation to ethanol.
abstract: A parametric study, with an initial load of 15%w/w of dry stover from white corn, was conducted to evaluate the sequential thermochemical hydrolysis (TH), enzymatic saccharification (ES) and fermentation of the whole slurry with ethanologenic Escherichia coli. The TH was designed to release the maximum amount of xylose with a concomitant formation of minimal amounts of furans. It was found that 29.0% or 93.2% of the xylan was recovered as free xylose at 130°C after 8 min in the presence of 1% or 2%w/w H2SO4 and produced only 0.06 or 0.44 g/L of total furans, respectively. After 24h of ES, 76.14-77.18 g/L of monosaccharides (pentoses and hexoses) were obtained. These slurries, which contained 0.03-0.26 g/L of total furans and 5.14-5.91 g/L of acetate, were fermented with 3.7 g/L of ethanologenic E. coli to produce 24.5-23.5 g/L of ethanol.
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos 62250, Mexico.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Vargas-Tah
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos 62250, Mexico.
authors.forename: Cessna L
authors.initials: CL
authors.lastname: Moss-Acosta
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos 62250, Mexico.
authors.forename: Berenice
authors.initials: B
authors.lastname: Trujillo-Martinez
authors.affiliation: Departamento de Ingeniería Genética, CINVESTAV IPN Unidad Irapuato, Km 9.6 Libramiento Norte Carretera Irapuato-León, C.P. 36821 Irapuato Gto., Mexico.
authors.forename: Axel
authors.initials: A
authors.lastname: Tiessen
authors.affiliation: Departamento de Ingeniería Genética, CINVESTAV IPN Unidad Irapuato, Km 9.6 Libramiento Norte Carretera Irapuato-León, C.P. 36821 Irapuato Gto., Mexico.
authors.forename: Edmundo
authors.initials: E
authors.lastname: Lozoya-Gloria
authors.affiliation: Departamento de Ingeniería Genética, CINVESTAV IPN Unidad Irapuato, Km 9.6 Libramiento Norte Carretera Irapuato-León, C.P. 36821 Irapuato Gto., Mexico.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Orencio-Trejo
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos 62250, Mexico.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Gosset
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos 62250, Mexico. Electronic address: alfredo@ibt.unam.mx.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Martinez
mesh_terms: D001709:Biotechnology / Q000379:methods*; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005663:Furans / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism; D006868:Hydrolysis; D009005:Monosaccharides / Q000378:metabolism; D010429:Pentoses / Q000378:metabolism; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D014994:Xylose / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004798:Enzymes; D005663:Furans; D006601:Hexoses; D009005:Monosaccharides; D010429:Pentoses; D000431:Ethanol; D014994:Xylose
keywords: Diluted acid pretreatment; Ethanol; Ethanologenic Escherichia coli; Sequential hydrolysis and fermentation; Stover from white corn