title: Construction and immunological evaluation of recombinant Lactobacillus plantarum expressing HN of Newcastle disease virus and DC- targeting peptide fusion protein.
abstract: Newcastle disease virus (NDV) has been considered as one of the most severe threats to poultry industry. In this study, we constructed a series of food grade recombinant Lactobacillus plantarum (L. plantarum) strains (RLP) synthesizing either virus hemagglutinin-neuraminidase protein (HN) alone or HN fused with DC cells targeting peptides (DCpep), named RLP(pSIP409-HN) and RLP (pSIP409-HN-DCpep), respectively. The immune responses and protective efficacy were then evaluated in chickens. Results showed that the presence of DCpep in RLP (pSIP409-HN-DCpep) group significantly increased the production of secretory immunoglobulin A (SIgA) in intestines and the percentages of CD3(+)CD4(+) T cells in spleen and peripheral blood leukocytes (P<0.05) compared to chickens immunized with RLP (pSIP409-HN). In addition, the similar enhancement effects were also observed with regard to trachea SIgA, T lymphocytes proliferation and survival rates after NDV challenge, even without significant statics differences. The results demonstrated the possibility to take use of DCpep as an immune adjuvant in the design of NDV vaccine.
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China.
authors.forename: Jingtao
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China.
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Ye
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China.
authors.forename: Chunwei
authors.initials: C
authors.lastname: Shi
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China. Electronic address: yangguilian@jlau.edu.cn.
authors.forename: Guilian
authors.initials: G
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China. Electronic address: wangchunfeng@jlau.edu.cn.
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D049109:Cell Proliferation; D002645:Chickens; D003713:Dendritic Cells / Q000276:immunology*; D015707:HN Protein / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D048191:Lactiplantibacillus plantarum / Q000276:immunology*; D009522:Newcastle disease virus / Q000276:immunology* / Q000472:pathogenicity; D010455:Peptides / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014774:Virulence
chemical_list: D000914:Antibodies, Viral; D015707:HN Protein; D010455:Peptides; D011993:Recombinant Fusion Proteins
keywords: DC-targeting peptide; HN protein; Immune response; L. plantarum; NDV