title: Development of chitosan/poly-γ-glutamic acid/pluronic/curcumin nanoparticles in chitosan dressings for wound regeneration.
abstract: The hydrophobic polyphenol curcumin has anti-inflammatory, antimicrobial, and wound-healing properties that warrant its pharmacological consideration. We report a curcumin nanoparticle with a tripolymeric composite that can be used as a delivery device for wound healing. The present composite nanoparticles were prepared with three biocompatible polymers of chitosan, poly-γ-glutamic acid, and pluronic using a simple ionic gelation technology. Pluronic was used to enhance the solubility of curcumin in chitosan/poly-γ-glutamic acid nanoparticles, leading to the incorporation of chitosan/poly-γ-glutamic acid/pluronic/curcumin nanoparticles into chitosan membranes, and reduced inflammation and bacterial infection during wound regeneration. Nanoparticles were of 193.1 ± 8.9 nm and had a zeta potential of 20.6 ± 2.4 mV. Moreover, in vitro analyses indicated controlled curcumin release in a simulated skin tissue model. Subsequent in vivo studies show that chitosan wound dressing containing chitosan/poly-γ-glutamic acid/pluronic/curcumin nanoparticles promoted neocollagen regeneration and tissue reconstruction. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 81-90, 2017.
authors.affiliation: Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.
authors.forename: Yu-Hsin
authors.initials: YH
authors.lastname: Lin
authors.affiliation: R&D Center, Bio-Medical Carbon Technology Co., Ltd, Taichung, Taiwan.
authors.forename: Jui-Hsiang
authors.initials: JH
authors.lastname: Lin
authors.affiliation: Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.
authors.forename: Ya-Shiuan
authors.initials: YS
authors.lastname: Hong
mesh_terms: D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry*; D020442:Poloxamer / Q000737:chemistry / Q000494:pharmacology; D011099:Polyglutamic Acid / Q000737:chemistry / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D020442:Poloxamer; D011099:Polyglutamic Acid; D048271:Chitosan; D003474:Curcumin
keywords: chitosan; curcumin; nanoparticles; pluronic; wound regeneration