title: High yield of functional metagenomic library from mangroves constructed in fosmid vector.
abstract: In the present study, metagenomic technique and fosmid vectors were used to construct a library of clones for exploring the biotechnological potential of mangrove soils by isolation of functional genes encoding hydrolytic enzymes. The library was built with genomic DNA from the soil samples of mangrove sediments and the functional screening of 1824 clones (~64 Mbp) was performed to detect the hydrolytic activity specific for cellulases, amylases (at acidic, neutral and basic pH), lipases/esterases, proteases, and nitrilases. Significant numbers of clones, positive for the tested enzyme activities were obtained. Our results indicate the importance and biotechnological potential of mangrove soils especially when compared to those obtained using other soil metagenomic libraries.
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
authors.forename: A C S
authors.initials: AC
authors.lastname: Gonçalves
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
authors.forename: A C F
authors.initials: AC
authors.lastname: dos Santos
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
authors.forename: T F
authors.initials: TF
authors.lastname: dos Santos
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
authors.forename: T B A
authors.initials: TB
authors.lastname: Pessoa
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
authors.forename: J C T
authors.initials: JC
authors.lastname: Dias
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil rezende.rachel@gmail.com.
authors.forename: R P
authors.initials: RP
authors.lastname: Rezende
mesh_terms: D000619:Aminohydrolases / Q000235:genetics; D000681:Amylases / Q000235:genetics; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D044602:Cellulases / Q000235:genetics; D003470:Culture Media / Q000737:chemistry; D004950:Esterases / Q000235:genetics; D015723:Gene Library; D005822:Genetic Vectors / Q000737:chemistry*; D054892:Metagenome; D059013:Microbial Consortia / Q000235:genetics; D010447:Peptide Hydrolases / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D053833:Wetlands
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D012987:Soil; D004950:Esterases; D000681:Amylases; D044602:Cellulases; D010447:Peptide Hydrolases; D000619:Aminohydrolases; C021867:nitrilase