title: Size and surface chemistry of nanoparticles lead to a variant behavior in the unfolding dynamics of human carbonic anhydrase.
abstract: The adsorption induced conformational changes of human carbonic anhydrase I (HCAi) and pseudo wild type human carbonic anhydrase II truncated at the 17th residue at the N-terminus (trHCAii) were studied in presence of nanoparticles of different sizes and polarities. Isothermal titration calorimetry (ITC) studies showed that the binding to apolar surfaces is affected by the nanoparticle size in combination with the inherent protein stability. 8-Anilino-1-naphthalenesulfonic acid (ANS) fluorescence revealed that HCAs adsorb to both hydrophilic and hydrophobic surfaces, however the dynamics of the unfolding at the nanoparticle surfaces drastically vary with the polarity. The size of the nanoparticles has opposite effects depending on the polarity of the nanoparticle surface. The apolar nanoparticles induce seconds timescale structural rearrangements whereas polar nanoparticles induce hours timescale structural rearrangements on the same charged HCA variant. Here, a simple model is proposed where the difference in the timescales of adsorption is correlated with the energy barriers for initial docking and structural rearrangements which are firmly regulated by the surface polarity. Near-UV circular dichorism (CD) further supports that both protein variants undergo structural rearrangements at the nanoparticle surfaces regardless of being "hard" or "soft". However, the conformational changes induced by the apolar surfaces differ for each HCA isoform and diverge from the previously reported effect of silica nanoparticles.
authors.affiliation: Department of Biochemistry and Structural Biology, Lund University, PO Box 124, SE 221 00, Lund, Sweden. Celia.Cabaleiro-Lago@biochemistry.lu.se Irem.Nasir@biochemistry.lu.se.
authors.forename: Irem
authors.initials: I
authors.lastname: Nasir
authors.forename: Martin
authors.initials: M
authors.lastname: Lundqvist
authors.forename: Celia
authors.initials: C
authors.lastname: Cabaleiro-Lago
mesh_terms: D002151:Calorimetry; D024401:Carbonic Anhydrase I / Q000737:chemistry*; D002942:Circular Dichroism; D006801:Humans; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D053758:Nanoparticles / Q000737:chemistry*; D058767:Protein Unfolding
chemical_list: D024401:Carbonic Anhydrase I