title: Newly Isolated Penicillium ramulosum N1 Is Excellent for Producing Protease-Resistant Acidophilic Xylanase.
abstract: Penicillium ramulosum N1 was isolated from decaying wood. This strain produces extracellular xylanases and cellulases. The highest activities of xylanases (250 U/ml) and carboxymethyl cellulose (CMCase; 6.5 U/ml) were produced when 1% barley straw was added as a carbon source. The optimum temperature and pH for xylanase activity was 55 and 3.0 °C, respectively. The xylanases exhibited strong protease resistance. CMCase revealed maximum activities at pH 3.0 and in the range of 60-70 °C. Filter paper activity was optimally active at pH 5.0 and 55 °C. The zymograms produced by the SDS-PAGE resolution of the crude enzymes indicated that there are four bands of protein with xylanase activity and three bands of proteins with endoglucanase. The results revealed that P. ramulosum N1 is a promising acidophilic and protease-resistant xylanase-producing microorganism that has great potential to be used in animal feed and food industry applications.
authors.affiliation: State Key Laboratory of Rice Biology and Institute of Insect Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, PR China.
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Lin
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Shen
authors.forename: Tingheng
authors.initials: T
authors.lastname: Zhu
authors.forename: Wensheng
authors.initials: W
authors.lastname: Qin
mesh_terms: D002480:Cellulase / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D010407:Penicillium / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification*; D010447:Peptide Hydrolases / Q000378:metabolism; D010802:Phylogeny; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D003470:Culture Media; D014990:Xylans; D002482:Cellulose; D014995:Xylosidases; D002480:Cellulase; C019826:carboxymethylcellulase; D010447:Peptide Hydrolases