title: Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney.
abstract: Here we showed that dietary NiCl2 in excess of 300 mg/kg caused the G2/M cell cycle arrest and the reduction of cell proportion at S phase. The G2/M cell cycle arrest was accompanied by up-regulation of phosphorylated ataxia telangiectasia mutated (p-ATM), p53, p-Chk1, p-Chk2, p21 protein expression and ATM, p53, p21, Chk1, Chk2 mRNA expression, and down-regulation of p-cdc25C, cdc2, cyclinB and proliferating cell nuclear antigen (PCNA) protein expression and the cdc25, cdc2, cyclinB, PCNA mRNA expression.
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an, China.
authors.forename: Hongrui
authors.initials: H
authors.lastname: Guo
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an, China.|College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an, China.|College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an, China.|College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an, China.|College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an, China.|College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: Junliang
authors.initials: J
authors.lastname: Deng
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an, China.|College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an, China.
authors.forename: Bangyuan
authors.initials: B
authors.lastname: Wu
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an, China.
authors.forename: Kejie
authors.initials: K
authors.lastname: Chen
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Deng
mesh_terms: D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins / Q000235:genetics / Q000378:metabolism; D002645:Chickens; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D007668:Kidney / Q000166:cytology* / Q000187:drug effects*; D009532:Nickel / Q000008:administration & dosage*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D015398:Signal Transduction; D025521:Tumor Suppressor Proteins / Q000378:metabolism
chemical_list: D025521:Tumor Suppressor Proteins; C022838:nickel chloride; D009532:Nickel; D011494:Protein Kinases; D064007:Ataxia Telangiectasia Mutated Proteins
keywords: G2/M cell cycle arrest; NiCl2; kidney; mRNA expression; protein expression