title: Review on complement analysis method and the roles of glycosaminoglycans in the complement system.
abstract: Complement system is composed of over 30 proteins and it plays important roles in self-defence and inflammation. There are three activation pathways, including classical pathway, alternative pathway and lectin pathway, in complement system, and they are associated with many diseases such as osteoarthritis and age-related macular degeneration. Modulation of the complement system may be a promising strategy in the treatment of related diseases. Glycosaminoglycans are anionic linear polysaccharides without branches. They are one kind of multi-functional macromolecules which have great potential in regulating complement system. This review is organized around two aspects between the introduction of complement system and the interaction of glycosaminoglycans with complement system. Three complement activation pathways and the biological significance were introduced first. Then functional analysis methods were compared to provide a strategy for potential glycosaminoglycans screen. Finally, the roles of glycosaminoglycans played in the complement system were summed up.
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, No. 44 Wenhuaxi Road, Jinan 250012, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, No. 44 Wenhuaxi Road, Jinan 250012, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, No. 44 Wenhuaxi Road, Jinan 250012, China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ijaz
authors.affiliation: Department of Hepatobiliary Surgery, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Shahbaz
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, No. 44 Wenhuaxi Road, Jinan 250012, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Lian
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, No. 44 Wenhuaxi Road, Jinan 250012, China. Electronic address: fswang@sdu.edu.cn.
authors.forename: Fengshan
authors.initials: F
authors.lastname: Wang
mesh_terms: D000818:Animals; D003165:Complement System Proteins / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism*; D006801:Humans
chemical_list: D006025:Glycosaminoglycans; D003165:Complement System Proteins
keywords: Analytical method; Anti-complement activity; Complement; Glycosaminoglycan