title: Conformation of carboxylated schizophyllan in aqueous solution.
abstract: Carboxylated schizophyllan (sclerox) samples of different degrees of oxidation were molecularly characterized by size exclusion chromatography equipped with a multi-angle light scattering detector (SEC-MALS) in 0.10 M aqueous NaCl solution. The molar mass distribution obtained by SEC-MALS shows that sclerox of low degree of oxidation is dissolved mainly as the trimer, whereas the trimer and single chain coexist in solution of sclerox of high degree of oxidation. The trimer of sclerox is much more flexible than the fully ordered triple helix of the parent schizophyllan and easily dissociates into single chains upon heating.
authors.affiliation: Division of Molecular Science, Faculty of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan. Electronic address: yoshiba@gunma-u.ac.jp.
authors.forename: Kazuto
authors.initials: K
authors.lastname: Yoshiba
authors.affiliation: Department of Macromolecular Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sato
authors.affiliation: Division of Molecular Science, Faculty of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Osumi
authors.affiliation: NOBIPOL, Department of Biotechnology, Norwegian University of Science and Technology (NTNU), Trondheim NO-7491, Norway.
authors.forename: Ann-Sissel T
authors.initials: AS
authors.lastname: Ulset
authors.affiliation: NOBIPOL, Department of Biotechnology, Norwegian University of Science and Technology (NTNU), Trondheim NO-7491, Norway.
authors.forename: Bjørn E
authors.initials: BE
authors.lastname: Christensen
keywords: Light scattering; Periodate oxidation; Schizophyllan; Size exclusion chromatography; Triple helix