title: Comparison of proteome typing and serotyping of Streptococcus parauberis isolates from olive flounder (Paralichthys olivaceus).
abstract: The olive flounder (Paralichthys olivaceus) is a cultivated marine species that is economically important in Korea and Japan. Several bacterial pathogens have caused severe mortalities in farmed olive flounder, especially Streptococcus parauberis. We collected 145 S. parauberis isolates from diseased olive flounders from 2003 to 2008 in Jeju Island, South Korea and characterized them by Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI TOF MS) and by serology. The serological analysis divided the isolates into serotype I (62.1%) and serotype II (36.6%) and the proteome analysis divided the isolates into cluster 1 (43.4%) and cluster 2 (56.6%). All cluster 1 isolates had serotype I, but cluster 2 consisted of serotype I (32.9%), serotype II (64.6%), and others (2.5%). Further detailed analysis of the mass spectra led to identification of several specific m/z peaks that enabled discrimination between cluster 1 and 2 and between serotype I and II within cluster 2. Our results suggest that MALDI TOF MS analysis has potential as an alternative method for the rapid and reliable identification of the fish pathogen S. parauberis.
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Si Won
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Ho Bin
authors.initials: HB
authors.lastname: Jang
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Jung Seok
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Se Pyeong
authors.initials: SP
authors.lastname: Im
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Jassy Mary S
authors.initials: JM
authors.lastname: Lazarte
authors.affiliation: Korea Fish Breeding Research Institute, 866, Haemajihaean-ro, Gujwa, Jeju-si, Jeju-do, Korea.
authors.forename: Jong Pyo
authors.initials: JP
authors.lastname: Seo
authors.affiliation: BluGen Korea, Mangmibeonyeong-ro 38beon-gil, Suyeong-gu, Busan, Korea.
authors.forename: Woo Jai
authors.initials: WJ
authors.lastname: Lee
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Jae Sung
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: jungts@gnu.ac.kr.
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Jung
mesh_terms: D000818:Animals; D005432:Flounder / Q000382:microbiology*; D007564:Japan; D007723:Korea; D020543:Proteome / Q000032:analysis; D065288:Serogroup; D012703:Serotyping / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013291:Streptococcus / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*
chemical_list: D020543:Proteome
keywords: MALDI TOF MS; Olive flounder (Paralichthys olivaceus); Streptococcus parauberis