title: Enhancement of solvent tolerance in Pseudomonas sp. BCNU 106 with trehalose.
abstract: As the solvent hyper-resistant Pseudomonas sp. BCNU 106 experiences limited growth with solvents, a strategy is therefore needed to allow better growth to broaden its performance in biotechnological applications. Pseudomonas sp. BCNU 106 was cultivated in a medium supplemented with 0·05 mol l(-1) trehalose, and the cell survival was observed during subsequent growth with 1% (v/v) toluene. Exogenously added trehalose was transported into the cells and conferred protection against toluene stress. BCNU 106 grown in the presence of exogenous trehalose showed higher solvent tolerance, it can thus have more potential for biotransformation and biodegradation.
  
  
  CONCLUSIONS
  This study shows that exogenously supplemented trehalose confers protection against toluene stress and enhances the bacterial cell growth in the presence of toluene. This is of importance to the mass cultivation of solvent-tolerant bacteria, where some of the growth-related limitations of solvent-tolerant bacteria can be overcome, and their performance in biotechnological applications for biotransformation and biodegradation broadened.
authors.affiliation: Department of Biology and Chemistry, Changwon National University, Changwon, Korea.
authors.forename: B R
authors.initials: BR
authors.lastname: Lim
authors.affiliation: Department of Biology and Chemistry, Changwon National University, Changwon, Korea.
authors.forename: H J
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: Department of Bioresource Sciences, Andong National University, Andong, Korea.
authors.forename: G-S
authors.initials: GS
authors.lastname: Kwon
authors.affiliation: Department of Biology and Chemistry, Changwon National University, Changwon, Korea.
authors.forename: W H
authors.initials: WH
authors.lastname: Joo
mesh_terms: D001673:Biodegradation, Environmental; D001709:Biotechnology; D001711:Biotransformation; D011549:Pseudomonas / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D012997:Solvents / Q000494:pharmacology*; D014050:Toluene / Q000494:pharmacology*; D014199:Trehalose / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D012997:Solvents; D014050:Toluene; D014199:Trehalose
keywords: Pseudomonas sp.; exogenous trehalose; stress tolerance; toluene tolerance; trehalose