title: A single gene all3940 (Dps) overexpression in Anabaena sp. PCC 7120 confers multiple abiotic stress tolerance via proteomic alterations.
abstract: DNA-binding proteins (Dps) induced during starvation play an important role in gene regulation and maintaining homeostasis in bacteria. The nitrogen-fixing cyanobacterium, Anabaena PCC7120, has four genes annotated as coding for Dps; however, the information on their physiological roles is limiting. One of the genes coding for Dps, 'all3940' was found to be induced under different abiotic stresses in Anabaena and upon overexpression enhanced the tolerance of Anabaena to a multitude of stresses, which included salinity, heat, heavy metals, pesticide, and nutrient starvation. On the other hand, mutation in the gene resulted in decreased growth of Anabaena. The modulation in the levels of All3940 in Anabaena, achieved either by overexpression of the protein or mutation of the gene, resulted in changes in the proteome, which correlated well with the physiological changes observed. Proteins required for varied physiological activities, such as photosynthesis, carbon-metabolism, oxidative stress alleviation, exhibited change in protein profile upon modulation of All3940 levels in Anabaena. This suggested a direct or an indirect effect of All3940 on the expression of the above stress-responsive proteins, thereby enhancing tolerance in Anabaena PCC7120. Thus, All3940, though categorized as a Dps, is possibly a general stress protein having a global role in regulating tolerance to multitude of stresses in Anabaena.
authors.affiliation: Molecular Biology Section, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221 005, India.|Plant Biotechnology, Rajiv Gandhi South Campus, Banaras Hindu University, Barkachha, Mirzapur, 231301, India.
authors.forename: Om Prakash
authors.initials: OP
authors.lastname: Narayan
authors.affiliation: Molecular Biology Section, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221 005, India.|Department of Biological Sciences, Indian Institute of Science Education and Research, Sector-81, Mohali, 140 306, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Kumari
authors.affiliation: Gujarat State Biotechnology Mission, Department of Science and Technology, Government of Gujarat, GandhiNagar, Gujarat, India.
authors.forename: Poonam
authors.initials: P
authors.lastname: Bhargava
authors.affiliation: Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India.
authors.forename: Hema
authors.initials: H
authors.lastname: Rajaram
authors.affiliation: Molecular Biology Section, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221 005, India. lcrbhu15@gmail.com.
authors.forename: Lal Chand
authors.initials: LC
authors.lastname: Rai
mesh_terms: D017033:Anabaena / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D015854:Up-Regulation
chemical_list: D004268:DNA-Binding Proteins; D010940:Plant Proteins; D020543:Proteome
keywords: Anabaena; Genetic engineering; MALDI-TOF/MS; Proteomics