title: Is disulphide/thiol ratio related to blood pressure in masked hypertension?
abstract: Dynamic thiol/disulphide homeostasis plays a critical role in numerous intracellular enzymatic pathways including antioxidant defence and detoxification. In this study, we sought to investigate dynamic thiol/disulphide homeostasis in patients with masked hypertension (MHT) and its relationship with blood pressure. Forty patients (23 men, 17 women) with newly diagnosed MHT and not yet on medical therapy, and 40 healthy volunteers (21 men, 19 women) were enrolled. Blood thiol/disulphide homeostasis was measured in both groups. Serum native and total thiol levels were measured using the novel, fully automated colorimetric method developed by Erel et al. Serum disulphide level was calculated as (serum total thiol - serum native thiol)/2. Native and total thiol levels (p = 0.001) and native thiol/total thiol ratio (p = 0.023) were found to be lower in patients with MHT when compared to those of the control group. Disulphide level and ratios of disulphide/native thiol and disulphide/total thiol were higher in patients with MHT than in the control group (p = 0.001). A positive correlation of systolic blood pressure (SBP) and diastolic blood pressure (DBP) was observed with disulphide/native thiol ratio (p < 0.001). Stepwise multivariable regression analysis showed disulphide/native thiol ratio to be an independent risk factor of SBP and DBP, and SBP to be an independent risk factor of disulphide/thiol ratio (p = 0.001). In this study, we found that dynamic thiol/disulphide homeostasis shifted towards disulphide formation due to thiol oxidation in patients with MHT. Prospective randomised controlled studies are required to elucidate whether abnormal thiol/disulphide status lies in the pathogenesis of MHT or is a consequence of MHT.
authors.affiliation: a Internal Medicine Department .
authors.forename: İhsan
authors.initials: İ
authors.lastname: Ateş
authors.affiliation: b Nephrology Department .
authors.forename: Nihal
authors.initials: N
authors.lastname: Ozkayar
authors.affiliation: a Internal Medicine Department .
authors.forename: Mustafa
authors.initials: M
authors.lastname: Altay
authors.affiliation: c Biochemistry Department , Ankara Numune Education and Research Hospital , Ankara , Turkey , and.|d Department of Biochemistry , Medical Faculty, Yildirim Beyazit University , Ankara , Turkey.
authors.forename: Fatma Meriç
authors.initials: FM
authors.lastname: Yilmaz
authors.affiliation: c Biochemistry Department , Ankara Numune Education and Research Hospital , Ankara , Turkey , and.
authors.forename: Canan
authors.initials: C
authors.lastname: Topçuoğlu
authors.affiliation: d Department of Biochemistry , Medical Faculty, Yildirim Beyazit University , Ankara , Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Alışık
authors.affiliation: d Department of Biochemistry , Medical Faculty, Yildirim Beyazit University , Ankara , Turkey.
authors.forename: Özcan
authors.initials: Ö
authors.lastname: Erel
authors.affiliation: b Nephrology Department .
authors.forename: Fatih
authors.initials: F
authors.lastname: Dede
mesh_terms: D000328:Adult; D001794:Blood Pressure / Q000502:physiology; D001795:Blood Pressure Determination; D016022:Case-Control Studies; D004220:Disulfides / Q000097:blood*; D005260:Female; D006706:Homeostasis; D006801:Humans; D008297:Male; D059468:Masked Hypertension / Q000097:blood* / Q000175:diagnosis / Q000503:physiopathology; D008875:Middle Aged; D011446:Prospective Studies; D012307:Risk Factors; D013438:Sulfhydryl Compounds / Q000097:blood*
chemical_list: D004220:Disulfides; D013438:Sulfhydryl Compounds
keywords: Dynamic thiol/disulphide; isolated ambulatory hypertension; oxidant radicals; oxidative stress; oxidised thiol