title: Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins.
abstract: The generation of genetically-modified organisms has been revolutionized by the development of new genome editing technologies based on the use of gene-specific nucleases, such as meganucleases, ZFNs, TALENs and CRISPRs-Cas9 systems. The most rapid and cost-effective way to generate genetically-modified animals is by microinjection of the nucleic acids encoding gene-specific nucleases into zygotes. However, the efficiency of the procedure can still be improved. In this work we aim to increase the efficiency of CRISPRs-Cas9 and TALENs homology-directed repair by using TALENs and Cas9 proteins, instead of mRNA, microinjected into rat and mouse zygotes along with long or short donor DNAs. We observed that Cas9 protein was more efficient at homology-directed repair than mRNA, while TALEN protein was less efficient than mRNA at inducing homology-directed repair. Our results indicate that the use of Cas9 protein could represent a simple and practical methodological alternative to Cas9 mRNA in the generation of genetically-modified rats and mice as well as probably some other mammals.
authors.affiliation: INSERM UMR 1064-ITUN; CHU de Nantes, Nantes F44093, France.|Platform Rat Transgenesis Immunophenomic, SFR François Bonamy, CNRS UMS3556 Nantes, F44093, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Ménoret
authors.affiliation: INSERM U1154, CNRS UMR7196, Structure and Instability of Genomes, Sorbonne Universités, Museum National d'Histoire Naturelle; CP26 57 rue Cuvier, F75005 Paris, France.
authors.forename: Anne
authors.initials: A
authors.lastname: De Cian
authors.affiliation: INSERM UMR 1064-ITUN; CHU de Nantes, Nantes F44093, France.|Platform Rat Transgenesis Immunophenomic, SFR François Bonamy, CNRS UMS3556 Nantes, F44093, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Tesson
authors.affiliation: INSERM UMR 1064-ITUN; CHU de Nantes, Nantes F44093, France.|Platform Rat Transgenesis Immunophenomic, SFR François Bonamy, CNRS UMS3556 Nantes, F44093, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Remy
authors.affiliation: INSERM UMR 1064-ITUN; CHU de Nantes, Nantes F44093, France.|Platform Rat Transgenesis Immunophenomic, SFR François Bonamy, CNRS UMS3556 Nantes, F44093, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Usal
authors.affiliation: INSERM U1154, CNRS UMR7196, Structure and Instability of Genomes, Sorbonne Universités, Museum National d'Histoire Naturelle; CP26 57 rue Cuvier, F75005 Paris, France.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Boulé
authors.affiliation: INSERM U1154, CNRS UMR7196, Structure and Instability of Genomes, Sorbonne Universités, Museum National d'Histoire Naturelle; CP26 57 rue Cuvier, F75005 Paris, France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Boix
authors.affiliation: genOway, Lyon F69007, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Fontanière
authors.affiliation: INSERM UMR 1064-ITUN; CHU de Nantes, Nantes F44093, France.
authors.forename: Alison
authors.initials: A
authors.lastname: Crénéguy
authors.affiliation: INSERM UMR 1064-ITUN; CHU de Nantes, Nantes F44093, France.
authors.forename: Tuan H
authors.initials: TH
authors.lastname: Nguyen
authors.affiliation: INSERM UMR 1064-ITUN; CHU de Nantes, Nantes F44093, France.
authors.forename: Lucas
authors.initials: L
authors.lastname: Brusselle
authors.affiliation: INSERM UMR 1064-ITUN; CHU de Nantes, Nantes F44093, France.|Platform Rat Transgenesis Immunophenomic, SFR François Bonamy, CNRS UMS3556 Nantes, F44093, France.
authors.forename: Reynald
authors.initials: R
authors.lastname: Thinard
authors.affiliation: Sorbonne Universities, University Pierre &Marie Curie, University Paris Descartes, Sorbonne Paris Cité, INSERM UMR_S 1138, Cordeliers Research Centre, 75006 Paris, France.|Institute of Cardiometabolism and Nutrition, University Pierre &Marie Curie, Boulevard de l'Hopital, 75013 Paris, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Gauguier
authors.affiliation: INSERM U1154, CNRS UMR7196, Structure and Instability of Genomes, Sorbonne Universités, Museum National d'Histoire Naturelle; CP26 57 rue Cuvier, F75005 Paris, France.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Concordet
authors.affiliation: genOway, Lyon F69007, France.
authors.forename: Yacine
authors.initials: Y
authors.lastname: Cherifi
authors.affiliation: genOway, Lyon F69007, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Fraichard
authors.affiliation: INSERM U1154, CNRS UMR7196, Structure and Instability of Genomes, Sorbonne Universités, Museum National d'Histoire Naturelle; CP26 57 rue Cuvier, F75005 Paris, France.
authors.forename: Carine
authors.initials: C
authors.lastname: Giovannangeli
authors.affiliation: INSERM UMR 1064-ITUN; CHU de Nantes, Nantes F44093, France.|Platform Rat Transgenesis Immunophenomic, SFR François Bonamy, CNRS UMS3556 Nantes, F44093, France.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Anegon
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008845:Microinjections; D015202:Protein Engineering; D051381:Rats; D017207:Rats, Sprague-Dawley; D059767:Recombinational DNA Repair / Q000235:genetics*; D015053:Zygote / Q000502:physiology*