title: Wheat WCBP1 encodes a putative copper-binding protein involved in stripe rust resistance and inhibition of leaf senescence.
abstract: BACKGROUND
  Stripe rust, a highly destructive foliar disease of wheat (Triticum aestivum), causes severe losses, which may be accompanied by reduced photosynthetic activity and accelerated leaf senescence.
  
  
  METHODS
  We used suppression subtractive hybridization (SSH) to examine the mechanisms of resistance in the resistant wheat line L693 (Reg. No. GP-972, PI 672538), which was derived from a lineage that includes a wide cross between common and Thinopyrum intermedium. Sequencing of an SSH cDNA library identified 112 expressed sequence tags.
  
  
  RESULTS
  In silico mapping placed one of these tags [GenBank: JK972238] on chromosome 1A. Primers based on [GenBank: JK972238] amplified a polymorphic band, which co-segregated with YrL693. We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval. Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.
  
  
  CONCLUSIONS
  Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.
  
  
  CONCLUSIONS
  The unique chromosomal location and expression mode of WCBP1 suggested that WCBP1 is the putative candidate gene of YrL693, which was involved in leaf senescence and photosynthesis related to plant responses to stripe rust infection during the grain-filling stage.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China. sadoneli@gmail.com.|Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. sadoneli@gmail.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China. tgliu@ippcaas.cn.
authors.forename: Taiguo
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China. wqchen112@ippcaas.cn.
authors.forename: Wanquan
authors.initials: W
authors.lastname: Chen
authors.affiliation: Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. 18481012@qq.com.
authors.forename: Shengfu
authors.initials: S
authors.lastname: Zhong
authors.affiliation: Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. zhyu@sicau.edu.cn.
authors.forename: Huaiyu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. zxtang@sicau.edu.cn.
authors.forename: Zongxiang
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Institute of Crop Genetics, Shanxi Academy of Agricultural Science, Taiyuan, Shanxi, 030031, China. wrczj@126.com.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Chang
authors.affiliation: Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. 731022275@qq.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. 691020460@qq.com.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. lilq88@qq.com.
authors.forename: Liqin
authors.initials: L
authors.lastname: Li
authors.affiliation: Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. 654786574@qq.com.
authors.forename: Hefei
authors.initials: H
authors.lastname: Rao
authors.affiliation: Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. lpg052000@aliyun.com.
authors.forename: Zhenglong
authors.initials: Z
authors.lastname: Ren
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China. lpglab@sicau.edu.cn.|Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. lpglab@sicau.edu.cn.
authors.forename: Peigao
authors.initials: P
authors.lastname: Luo
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D020090:Chromosome Segregation; D003001:Cloning, Molecular; D060467:Disease Resistance; D020224:Expressed Sequence Tags; D005453:Fluorescence; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008040:Genetic Linkage; D005838:Genotype; D008969:Molecular Sequence Data; D010788:Photosynthesis; D045332:Photosystem II Protein Complex / Q000378:metabolism; D010802:Phylogeny; D020161:Physical Chromosome Mapping; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000502:physiology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D044224:Pseudomonas syringae / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D036226:Seedlings / Q000382:microbiology; D014908:Triticum / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*
chemical_list: D002352:Carrier Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; C023548:copper-binding protein; D002734:Chlorophyll