title: [The Xanthine Oxidase Inhibitory Activity and Hypouricemic Effects of Crude Drugs Obtained from the Silkworm in Mice].
abstract: This study evaluated the effects of crude drugs obtained from the silkworm in mice with oxonic acid-induced hyperuricemia using xanthine oxidase inhibitory activity and plasma uric acid levels. The plasma uric acid level was analyzed using an improved HPLC with UV detection (HPLC-UV) method, which enabled high-sensitivity analysis of a microliter of plasma. Using this method, we evaluated natural products administered orally to the hypouricemic mice. The plasma uric acid level of mice administered a water-soluble extract from silkworm larvae with botrytis (used in traditional Chinese medicine to reduce wind, lower blood pressure, and change platelet coagulation) was significantly lower than in the control group 1, 2, and 3 h after treatment. In addition, water soluble extracts from a fungus (NBRC 31161) metabolite and silkworm pupae and larvae reduced the plasma uric acid levels in mice compared with the control group.
authors.affiliation: Faculty of Pharmaceutical Sciences, Setsunan University.
authors.forename: Ryuichirou
authors.initials: R
authors.lastname: Tanaka
authors.forename: Yuuma
authors.initials: Y
authors.lastname: Miyata
authors.forename: Naoki
authors.initials: N
authors.lastname: Minakuchi
authors.forename: Ayako
authors.initials: A
authors.lastname: Murakami
authors.forename: Fumitoshi
authors.initials: F
authors.lastname: Sakazaki
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001688:Biological Products / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood; D001794:Blood Pressure / Q000187:drug effects; D012831:Bombyx / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D045424:Complex Mixtures / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use*; D003877:Dermatitis, Contact; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors; D033461:Hyperuricemia / Q000139:chemically induced / Q000175:diagnosis / Q000188:drug therapy*; D051379:Mice; D010094:Oxonic Acid; D010974:Platelet Aggregation / Q000187:drug effects; D014527:Uric Acid / Q000097:blood; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
chemical_list: D001688:Biological Products; D015415:Biomarkers; D045424:Complex Mixtures; D004791:Enzyme Inhibitors; D014527:Uric Acid; D010094:Oxonic Acid; D014969:Xanthine Oxidase