title: The Role of a Destabilized Membrane for OMP Insertion.
abstract: Here we describe the procedures used in our laboratory for the in vitro investigation of the apparent folding kinetics as well as the folding efficiencies of outer membrane proteins (OMPs). Because microbial OMPs display a change in their gel migration upon folding, the usage of traditional gel electrophoresis is a standard method of folding analysis. Additional aspects of the method we detail herein include the preparation and storage of OMP stocks, the setup procedures for a folding reaction, and the analysis of fraction folded from scanned gel images.
authors.affiliation: T. C. Jenkins Department of Biophysics, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, 21218, USA.
authors.forename: Ashlee M
authors.initials: AM
authors.lastname: Plummer
authors.affiliation: T. C. Jenkins Department of Biophysics, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, 21218, USA.
authors.forename: Dennis
authors.initials: D
authors.lastname: Gessmann
authors.affiliation: T. C. Jenkins Department of Biophysics, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, 21218, USA. karen.fleming@jhu.edu.
authors.forename: Karen G
authors.initials: KG
authors.lastname: Fleming
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002479:Inclusion Bodies / Q000378:metabolism; D007700:Kinetics; D057054:Molecular Imaging; D017510:Protein Folding; D053835:Unilamellar Liposomes / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D053835:Unilamellar Liposomes
keywords: BamA; Extrusion; Kinetics; Large unilamellar vesicles; Membrane protein folding; OMP