title: Prevention of cyclophosphamide-induced hepatotoxicity and genotoxicity: Effect of an L-cysteine based oxovanadium(IV) complex on oxidative stress and DNA damage.
abstract: Vanadium has been emerged as a promising agent owing to its ability to prevent several types of cancer. This study was aimed to investigate the protective role of an organovanadium complex, viz., oxovanadium(IV)-L-cysteine methyl ester (VC-IV) against cyclophosphamide (CP)-induced hepatotoxicity and genotoxicity in mice. Oral administration of VC-IV quite effectively ameliorated CP-induced histopathological lesions and reduced levels of alanine transaminase, aspartate transaminase and alkaline phosphatase. In addition, VC-IV significantly attenuated CP-induced oxidative stress in the liver as evident from levels of reactive oxygen species, nitric oxide and lipid peroxidation. Restoration of glutathione level and activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase) were also observed upon VC-IV administration. Moreover, VC-IV significantly mitigated CP-induced chromosomal aberrations, micronuclei formation, DNA fragmentation and apoptosis in bone marrow cells and DNA damage in lymphocytes. The present study showed that VC-IV could provide adequate protection against CP-induced hepatotoxicity and genotoxicity in vivo.
authors.affiliation: Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, Kolkata 700026, West Bengal, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Basu
authors.affiliation: Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, Kolkata 700026, West Bengal, India.
authors.forename: Arin
authors.initials: A
authors.lastname: Bhattacharjee
authors.affiliation: Division of Microbiology, Department of Pharmaceutical Technology, Jadavpur University, 188, Raja S. C. Mullick Road, Kolkata 700032, West Bengal, India.
authors.forename: Amalesh
authors.initials: A
authors.lastname: Samanta
authors.affiliation: Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, Kolkata 700026, West Bengal, India. Electronic address: sudinb19572004@yahoo.co.in.
authors.forename: Sudin
authors.initials: S
authors.lastname: Bhattacharya
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D002869:Chromosome Aberrations / Q000139:chemically induced / Q000187:drug effects*; D003520:Cyclophosphamide / Q000008:administration & dosage* / Q000633:toxicity; D003545:Cysteine / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D009942:Organometallic Compounds / Q000008:administration & dosage* / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects; D014638:Vanadates / Q000737:chemistry*
chemical_list: D009942:Organometallic Compounds; C075743:oxovanadium IV; D014638:Vanadates; D003520:Cyclophosphamide; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D003545:Cysteine
keywords: Apoptosis; Chemoprotection; Cyclophosphamide; DNA damage; Histopathology; Oxidative stress