title: Central metabolic pathways of Aureobasidium pullulans CGMCC1234 for pullulan production.
abstract: With the purpose of understanding the metabolic network of Aureobasidium pullulans, the central metabolic pathways were confirmed by the activities of the key enzymes involved in different pathways. The effect of different iodoacetic acid concentrations on pullulan fermentation was also investigated in this paper. The activities of phosphofructokinases and glucose-6-phosphate dehydrogenase existed in A. pullulans CGMCC1234, whereas 2-keto-3-deoxy-6-phosphogluconate aldolase activity was not detected. We proposed that the central metabolic pathways of A. pullulans CGMCC1234 included EMP and PPP, but no ED. Pullulan production declined fast as the iodoacetic acid increased, while cell growth offered upgrade firstly than descending latter tendency. Compared to the control group, the ratio of ATP/ADP of 0.60 mM iodoacetic acid group was lower at different stages of pullulan fermentation. The findings revealed that low concentration of iodoacetic acid might impel carbon flux flow toward the PPP, but reduce the flux of the EMP.
authors.affiliation: National R&D Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Long
authors.initials: L
authors.lastname: Sheng
authors.affiliation: Hsingwu Business and Tourism School, Shanghai Lida Polytechnic Institute, Shanghai, China; The State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: The State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
authors.forename: Qunyi
authors.initials: Q
authors.lastname: Tong
authors.affiliation: National R&D Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address: mameihuhn@163.com.
authors.forename: Meihu
authors.initials: M
authors.lastname: Ma
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001203:Ascomycota / Q000201:enzymology / Q000378:metabolism*; D005285:Fermentation; D005936:Glucans / Q000378:metabolism*; D019807:Iodoacetic Acid / Q000378:metabolism; D053858:Metabolic Networks and Pathways
chemical_list: D005936:Glucans; D000255:Adenosine Triphosphate; C009109:pullulan; D019807:Iodoacetic Acid
keywords: Aureobasidium pullulans; Central metabolic; Pullulan