title: Scaffolds for Controlled Release of Cartilage Growth Factors.
abstract: In recent years, cell-based therapies using adult stem cells have attracted considerable interest in regenerative medicine. A tissue-engineered construct for cartilage repair should provide a support for the cell and allow sustained in situ delivery of bioactive factors capable of inducing cell differentiation into chondrocytes. Pharmacologically active microcarriers (PAMs), made of biodegradable and biocompatible poly (D,L-lactide-co-glycolide acid) (PLGA), are a unique system which combines these properties in an adaptable and simple microdevice. This device relies on nanoprecipitation of proteins encapsulated in polymeric microspheres with a solid in oil in water emulsion-solvent evaporation process, and their subsequent coating with extracellular matrix protein molecules. Here, we describe their preparation process, and some of their characterization methods for an application in cartilage tissue engineering.
authors.affiliation: Equipe Matériaux Avancés pour la Catalyse et la Santé, Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-ENSCM-UM2-UM1, 8 rue de l'Ecole Normale, 34296, Montpellier, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Morille
authors.affiliation: Micro et Nanomédecines Biomimétiques (MINT), LUNAM Université, 49933, Angers, France.|INSERM, U1066, 49933, Angers, France.
authors.forename: Marie-Claire
authors.initials: MC
authors.lastname: Venier-Julienne
authors.affiliation: Micro et Nanomédecines Biomimétiques (MINT), LUNAM Université, 49933, Angers, France. claudia.montero-menei@univ-angers.fr.|INSERM, U1066, 49933, Angers, France. claudia.montero-menei@univ-angers.fr.
authors.forename: Claudia N
authors.initials: CN
authors.lastname: Montero-Menei
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000637:transplantation; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism / Q000637:transplantation; D020219:Chondrogenesis / Q000187:drug effects*; D003692:Delayed-Action Preparations; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage* / Q000737:chemistry; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D050997:Nanomedicine; D011108:Polymers / Q000737:chemistry*; D012038:Regeneration / Q000187:drug effects; D044968:Regenerative Medicine / Q000379:methods*; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D053782:Transforming Growth Factor beta3 / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D003692:Delayed-Action Preparations; D036341:Intercellular Signaling Peptides and Proteins; D011108:Polymers; D053782:Transforming Growth Factor beta3
keywords: Chondrogenic differentiation; Mesenchymal stem cells; Pharmacologically active microcarriers; Protein encapsulation; Transforming growth factor