title: miR-381 Regulates Neural Stem Cell Proliferation and Differentiation via Regulating Hes1 Expression.
abstract: Neural stem cells are self-renewing, multipotent and undifferentiated precursors that retain the capacity for differentiation into both glial (astrocytes and oligodendrocytes) and neuronal lineages. Neural stem cells offer cell-based therapies for neurological disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease and spinal cord injuries. However, their cellular behavior is poorly understood. MicroRNAs (miRNAs) are a class of small noncoding RNAs involved in cell development, proliferation and differentiation through regulating gene expression at post-transcriptional level. The role of miR-381 in the development of neural stem cells remains unknown. In this study, we showed that overexpression of miR-381 promoted neural stem cells proliferation. It induced the neural stem cells differentiation to neurons and inhibited their differentiation to astrocytes. Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells. Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons. In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Respiratory, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Yan
authors.affiliation: Department of anatomy, Harbin Medical University, Harbin, 150081, PR China.
authors.forename: Baoquan
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.
authors.forename: Xuedan
authors.initials: X
authors.lastname: Nie
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.
authors.forename: Jiaolin
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin, 150081, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics*; D035683:MicroRNAs / Q000502:physiology*; D058953:Neural Stem Cells / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D000072056:Transcription Factor HES-1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018398:Homeodomain Proteins; D035683:MicroRNAs; D000072056:Transcription Factor HES-1; C489735:Hes1 protein, rat; C000608180:MIRN381 microRNA, rat