title: Laser Microdissection-Mediated Isolation and In Vitro Transcriptional Amplification of Plant RNA.
abstract: Laser microdissection of cells allows for isolation of specific cells of interest for downstream analyses including transcriptional profiling. Plant cells present unique challenges for laser microdissection due to their cellulosic cell walls and large vacuoles. Here we present protocols for plant tissue preparation, laser microdissection of select plant cells, and linear amplification of RNA from dissected cells. Linear amplification of RNA from dissected cells allows sufficient RNA for subsequent quantitative analysis by RT-PCR, microarray, or RNA sequencing.
authors.affiliation: University of California, Berkeley, California.|Regional Center for Biotechnology, Faridabad, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Chandran
authors.affiliation: Cornell University, Ithaca, New York.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Scanlon
authors.affiliation: Iowa State University, Ames, Iowa.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ohtsu
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
authors.forename: Marja C P
authors.initials: MCP
authors.lastname: Timmermans
authors.affiliation: Iowa State University, Ames, Iowa.
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Schnable
authors.affiliation: University of California, Berkeley, California.
authors.forename: Mary C
authors.initials: MC
authors.lastname: Wildermuth
mesh_terms: D061346:Laser Capture Microdissection / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D059828:Plant Cells; D018749:RNA, Plant / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D018749:RNA, Plant
keywords: RNA amplification; laser microdissection; plants; transcriptomics