title: Effect of dietary manganese on antioxidant status and expression levels of heat-shock proteins and factors in tissues of laying broiler breeders under normal and high environmental temperatures.
abstract: To investigate the effect of Mn on antioxidant status and expression levels of heat-shock proteins/factors in tissues of laying broiler breeders subjected to heat challenge, we used a completely randomised design (n 6) with a factorial arrangement of 2 environmental temperatures (normal, 21 (sem 1)°C and high, 32 (sem 1)°C)×3 dietary Mn treatments (an Mn-unsupplemented basal diet (CON), or a basal diet supplemented with 120 mg Mn/kg diet as inorganic Mn sulphate (iMn) or organic Mn proteinate (oMn)). There were no interactions (P>0·10) between environmental temperature and dietary Mn in all of the measured indices. High temperature decreased (P<0·003) Mn content, and also tended (P=0·07) to decrease copper zinc superoxide dismutase (CuZnSOD) activity in the liver and heart. However, an increased manganese superoxide dismutase (MnSOD) activity (P<0·05) and a slight increase of malondialdehyde level (P=0·06) were detected in breast muscle. Up-regulated (P<0·05) expression levels of heat-shock factor 1 (HSF1) and HSF3 mRNA and heat-shock protein 70 (HSP70) mRNA and protein were found in all three tissues. Broiler breeders fed either iMn or oMn had higher tissue Mn content (P<0·0001), heart MnSOD and CuZnSOD activities (P<0·01) and breast muscle MnSOD protein levels (P<0·05), and lower (P<0·05) breast muscle HSP70 mRNA and protein levels than those fed CON. Broiler breeders fed oMn had higher (P<0·03) bone Mn content than those fed iMn. These results indicate that high temperature decreases Mn retention and increases HSP70 and HSF1, HSF3 expression levels in tissues of laying broiler breeders. Furthermore, dietary supplementation with Mn in either source may enhance heart antioxidant ability and inhibit the expression of HSP70 in breast muscle. Finally, the organic Mn appears to be more available than inorganic Mn for bone in laying broiler breeders regardless of environmental temperatures.
authors.affiliation: 1Mineral Nutrition Research Division,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,People's Republic of China.
authors.forename: Yong-Wen
authors.initials: YW
authors.lastname: Zhu
authors.affiliation: 1Mineral Nutrition Research Division,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
authors.affiliation: 1Mineral Nutrition Research Division,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,People's Republic of China.
authors.forename: Wen-Xiang
authors.initials: WX
authors.lastname: Li
authors.affiliation: 1Mineral Nutrition Research Division,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,People's Republic of China.
authors.forename: Li-Yang
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: 2College of Animal Science and Technology,China Agricultural University,Beijing 100193,People's Republic of China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Ji
authors.affiliation: 3Department of Animal Science,North Carolina State University,Raleigh,NC 27695,USA.
authors.forename: Xi
authors.initials: X
authors.lastname: Lin
authors.affiliation: 3Department of Animal Science,North Carolina State University,Raleigh,NC 27695,USA.
authors.forename: Hsiao-Ching
authors.initials: HC
authors.lastname: Liu
authors.affiliation: 3Department of Animal Science,North Carolina State University,Raleigh,NC 27695,USA.
authors.forename: Jack
authors.initials: J
authors.lastname: Odle
authors.affiliation: 1Mineral Nutrition Research Division,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,People's Republic of China.
authors.forename: Xu-Gang
authors.initials: XG
authors.lastname: Luo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002645:Chickens; D004032:Diet; D005260:Female; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D008099:Liver / Q000201:enzymology; D008315:Malondialdehyde / Q000378:metabolism; D008345:Manganese / Q000008:administration & dosage* / Q000493:pharmacokinetics; D009206:Myocardium / Q000201:enzymology; D012313:RNA / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013696:Temperature; D014018:Tissue Distribution
chemical_list: D000975:Antioxidants; D006360:Heat-Shock Proteins; D008345:Manganese; D008315:Malondialdehyde; D012313:RNA; D013482:Superoxide Dismutase
keywords: Antioxidant status; Broiler breeders; CON Mn-unsupplemented basal diet; CuZnSOD copper zinc superoxide dismutase; HSF1 and HSF3
                  heat-shock factors 1 and 3; HSP70 and HSP90 heat-shock proteins 70 and 90; HT high temperature; Heat stress; Heat-shock proteins/factors; MDA malondialdehyde; Manganese; MnSOD manganese superoxide dismutase; NT normal temperature; ROS reactive oxygen species; SOD superoxide dismutase; iMn basal diet supplemented with 120 mg Mn/kg as inorganic Mn; oMn basal diet supplemented with 120 mg Mn/kg as organic Mn