title: Thermoresponsive release of viable microfiltrated Circulating Tumor Cells (CTCs) for precision medicine applications.
abstract: Stimulus responsive release of Circulating Tumor Cells (CTCs), with high recovery rates from their capture platform, is highly desirable for off-chip analyses. Here, we present a temperature responsive polymer coating method to achieve both release as well as culture of viable CTCs captured from patient blood samples.
authors.affiliation: Department of Pathology, University of Miami Miller School of Medicine, Miami, FL 33136, USA. A.agarwal2@miami.edu.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ao
authors.affiliation: Departments of Oncology and Pathology, and the Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, USA.
authors.forename: Erika
authors.initials: E
authors.lastname: Parasido
authors.affiliation: Department of Pathology, University of Miami Miller School of Medicine, Miami, FL 33136, USA. A.agarwal2@miami.edu.
authors.forename: Siddarth
authors.initials: S
authors.lastname: Rawal
authors.affiliation: Department of Pathology, University of Miami Miller School of Medicine, Miami, FL 33136, USA. A.agarwal2@miami.edu.
authors.forename: Anthony
authors.initials: A
authors.lastname: Williams
authors.affiliation: Departments of Oncology and Pathology, and the Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Schlegel
authors.affiliation: Departments of Oncology and Pathology, and the Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Liu
authors.affiliation: Departments of Oncology and Pathology, and the Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, USA.
authors.forename: Chris
authors.initials: C
authors.lastname: Albanese
authors.affiliation: Department of Pathology, University of Miami Miller School of Medicine, Miami, FL 33136, USA. A.agarwal2@miami.edu.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Cote
authors.affiliation: Department of Pathology, University of Miami Miller School of Medicine, Miami, FL 33136, USA. A.agarwal2@miami.edu and Department of Biomedical Engineering, University of Miami, USA.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Agarwal
authors.affiliation: Department of Pathology, University of Miami Miller School of Medicine, Miami, FL 33136, USA. A.agarwal2@miami.edu.
authors.forename: Ram H
authors.initials: RH
authors.lastname: Datar
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D046210:Microfluidic Analytical Techniques; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D011108:Polymers / Q000737:chemistry; D057285:Precision Medicine; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D011108:Polymers; C052970:poly-N-isopropylacrylamide