title: Comparison of three real-time PCR assays for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in young pregnant women.
abstract: We compared 3 commercial real-time PCR assays, the Abbott RealTime CT/NG, the cobas® 4800 CT/NG, and the Cepheid Xpert® CT/NG, for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in vaginal swabs collected prospectively from pregnant women aged <25 years. The overall agreement among 2 assays ranged from 98.9% to 99.5% with a kappa score between 0.94 and 0.97 for C. trachomatis. For N. gonorrhoeae, the overall agreement was 100%. All kits allowed prompt and specific results for C. trachomatis and N. gonorrhoeae in young pregnant women.
authors.affiliation: Univ. Bordeaux, USC EA 3671 Infections Humaines à Mycoplasmes et à Chlamydiae, Bordeaux, France; INRA, USC EA 3671 Infections Humaines à Mycoplasmes et à Chlamydiae, Bordeaux, France; Centre Hospitalier Universitaire de Bordeaux, Laboratoire de Bactériologie, Bordeaux, France. Electronic address: olivia.peuchant@u-bordeaux.fr.
authors.forename: Olivia
authors.initials: O
authors.lastname: Peuchant
authors.affiliation: Univ. Bordeaux, USC EA 3671 Infections Humaines à Mycoplasmes et à Chlamydiae, Bordeaux, France; INRA, USC EA 3671 Infections Humaines à Mycoplasmes et à Chlamydiae, Bordeaux, France.
authors.forename: Sabrina
authors.initials: S
authors.lastname: de Diego
authors.affiliation: Univ. Bordeaux, USC EA 3671 Infections Humaines à Mycoplasmes et à Chlamydiae, Bordeaux, France; INRA, USC EA 3671 Infections Humaines à Mycoplasmes et à Chlamydiae, Bordeaux, France.
authors.forename: Chloé
authors.initials: C
authors.lastname: Le Roy
authors.affiliation: Centre Hospitalier Universitaire de Bordeaux, Service de Gynécologie Obstétrique, Bordeaux, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Frantz-Blancpain
authors.affiliation: Centre Hospitalier Universitaire de Bordeaux, Service de Gynécologie Obstétrique, Bordeaux, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Hocké
authors.affiliation: Univ. Bordeaux, USC EA 3671 Infections Humaines à Mycoplasmes et à Chlamydiae, Bordeaux, France; INRA, USC EA 3671 Infections Humaines à Mycoplasmes et à Chlamydiae, Bordeaux, France; Centre Hospitalier Universitaire de Bordeaux, Laboratoire de Bactériologie, Bordeaux, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Bébéar
authors.affiliation: Univ. Bordeaux, USC EA 3671 Infections Humaines à Mycoplasmes et à Chlamydiae, Bordeaux, France; INRA, USC EA 3671 Infections Humaines à Mycoplasmes et à Chlamydiae, Bordeaux, France; Centre Hospitalier Universitaire de Bordeaux, Laboratoire de Bactériologie, Bordeaux, France.
authors.forename: Bertille
authors.initials: B
authors.lastname: de Barbeyrac
mesh_terms: D000293:Adolescent; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D005260:Female; D006069:Gonorrhea / Q000175:diagnosis*; D006801:Humans; D008219:Lymphogranuloma Venereum / Q000175:diagnosis*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis*; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014621:Vagina / Q000382:microbiology; D055815:Young Adult
keywords: Chlamydia trachomatis; Neisseria gonorrhoeae; Pregnant women; Real-time PCRs; Vaginal swabs