title: Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI).
abstract: The limits to optical resolution and the challenge of identifying specific protein populations in transmission electron microscopy have been obstacles in cell biology. Many phenomena cannot be explained by in vitro analysis in simplified systems and need additional structural information in situ, particularly in the range between 1 nm and 0.1 µm, in order to be fully understood. Here, electron spectroscopic imaging, a transmission electron microscopy technique that allows simultaneous mapping of the distribution of proteins and nucleic acids, and an expression tag, miniSOG, are combined to study the structure and organization of DNA double-strand break repair foci.
authors.affiliation: Department of Oncology, Faculty of Dentistry and Medicine, University of Alberta; hilmar@ualberta.ca.
authors.forename: Hilmar
authors.initials: H
authors.lastname: Strickfaden
authors.affiliation: Department of Oncology, Faculty of Dentistry and Medicine, University of Alberta.
authors.forename: Zhi Zhong
authors.initials: ZZ
authors.lastname: Xu
authors.affiliation: Department of Oncology, Faculty of Dentistry and Medicine, University of Alberta.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Hendzel
mesh_terms: D045744:Cell Line, Tumor; D002843:Chromatin / Q000737:chemistry; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D006801:Humans; D046529:Microscopy, Electron, Transmission; D046032:Microscopy, Energy-Filtering Transmission Electron / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D026082:Singlet Oxygen / Q000737:chemistry*
chemical_list: D002843:Chromatin; D011506:Proteins; D026082:Singlet Oxygen