title: Fluorescence-Based Approaches for Quantitative Assessment of Protein Carbonylation, Protein Disulfides, and Protein Conformation in Biological Tissues.
abstract: Protein oxidation and misfolding have been considered as key players for progression of aging and etiology of various pathological conditions. However, few attempts have been made to develop sensitive and reproducible assays to quantify the changes in protein oxidation and alteration in structure. Here we describe three distinct fluorescence-based assays to quantify changes in protein oxidation, namely carbonylation and disulfides and alteration in protein surface hydrophobicity as a reporter for protein conformation. These techniques will provide investigators the opportunity to address important biological questions in their experimental models.
authors.affiliation: UT Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390, USA. asish.chaudhuri@utsouthwestern.edu.|Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX, 78245, USA. asish.chaudhuri@utsouthwestern.edu.|Departments of Biochemistry, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229, USA. asish.chaudhuri@utsouthwestern.edu.|South Texas Veterans Health Care System, San Antonio, TX, 78229, USA. asish.chaudhuri@utsouthwestern.edu.
authors.forename: Asish R
authors.initials: AR
authors.lastname: Chaudhuri
authors.affiliation: Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX, 78245, USA.
authors.forename: Rochelle
authors.initials: R
authors.lastname: Wei
authors.affiliation: Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX, 78245, USA.|Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229, USA.
authors.forename: Arunabh
authors.initials: A
authors.lastname: Bhattacharya
authors.affiliation: Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX, 78245, USA.|Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Hamilton
mesh_terms: D004220:Disulfides; D005453:Fluorescence; D061848:Optical Imaging / Q000379:methods*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D050050:Protein Carbonylation; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D004220:Disulfides; D011506:Proteins
keywords: Carbonylation; Disulfide; Oxidative stress; Protein oxidation