title: Novel JAZ co-operativity and unexpected JA dynamics underpin Arabidopsis defence responses to Pseudomonas syringae infection.
abstract: Pathogens target phytohormone signalling pathways to promote disease. Plants deploy salicylic acid (SA)-mediated defences against biotrophs. Pathogens antagonize SA immunity by activating jasmonate signalling, for example Pseudomonas syringae pv. tomato DC3000 produces coronatine (COR), a jasmonic acid (JA) mimic. This study found unexpected dynamics between SA, JA and COR and co-operation between JAZ jasmonate repressor proteins during DC3000 infection. We used a systems-based approach involving targeted hormone profiling, high-temporal-resolution micro-array analysis, reverse genetics and mRNA-seq. Unexpectedly, foliar JA did not accumulate until late in the infection process and was higher in leaves challenged with COR-deficient P. syringae or in the more resistant JA receptor mutant coi1. JAZ regulation was complex and COR alone was insufficient to sustainably induce JAZs. JAZs contribute to early basal and subsequent secondary plant defence responses. We showed that JAZ5 and JAZ10 specifically co-operate to restrict COR cytotoxicity and pathogen growth through a complex transcriptional reprogramming that does not involve the basic helix-loop-helix transcription factors MYC2 and related MYC3 and MYC4 previously shown to restrict pathogen growth. mRNA-seq predicts compromised SA signalling in a jaz5/10 mutant and rapid suppression of JA-related components on bacterial infection.
authors.affiliation: Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK.
authors.forename: Marta
authors.initials: M
authors.lastname: de Torres Zabala
authors.affiliation: College of Biological Sciences, China Agricultural University, Beijing, 100093, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhai
authors.affiliation: Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK.
authors.forename: Siddharth
authors.initials: S
authors.lastname: Jayaraman
authors.affiliation: Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK.
authors.forename: Garoufalia
authors.initials: G
authors.lastname: Eleftheriadou
authors.affiliation: Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Winsbury
authors.affiliation: Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK.
authors.forename: Ron
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Plant Biology, University of Minnesota, Saint Paul, MN, 55108, USA.
authors.forename: William
authors.initials: W
authors.lastname: Truman
authors.affiliation: College of Biological Sciences, China Agricultural University, Beijing, 100093, China.
authors.forename: Saijung
authors.initials: S
authors.lastname: Tang
authors.affiliation: Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Smirnoff
authors.affiliation: Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK.
authors.forename: Murray
authors.initials: M
authors.lastname: Grant
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000187:drug effects / Q000235:genetics; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007192:Indenes / Q000494:pharmacology; D007532:Isoleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D054883:Oxylipins / Q000378:metabolism*; D010641:Phenotype; D010935:Plant Diseases / Q000382:microbiology*; D010937:Plant Growth Regulators / Q000378:metabolism; D044224:Pseudomonas syringae / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects; D014774:Virulence / Q000187:drug effects
chemical_list: D000596:Amino Acids; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D007192:Indenes; D054883:Oxylipins; D010937:Plant Growth Regulators; D012333:RNA, Messenger; C532883:jasmonoyl-isoleucine; D007532:Isoleucine; C058487:coronatine; C011006:jasmonic acid
keywords: Arabidopsis thaliana; JAZ; Pseudomonas syringae; hormone; jasmonic acid (JA)