title: Proteomic analysis of Eimeria acervulina sporozoite proteins interaction with duodenal epithelial cells by shotgun LC-MS/MS.
abstract: Although it has been known for many years that Eimeria acervulina (E. acervulina) initiates infection by invading the duodenal epithelial cells of chicken, the key protein molecules and the mechanisms of the parasite in invading are unknow. In this study, we found that 85 proteins of E. acervulina could bind with the chicken duodenal epithelial cells from Eimeria protein database. Among them, sixteen were identified only in Eimeria spp. correlation with invasion and evasion and 69 proteins were found in Eimeria spp. with more than 2 unique pep count. Nine out of the 16 proteins and 41 out of the 69 proteins were annotated according to Gene Ontology Annotation in terms of molecular function, biological process, and cellular localization. Most of the 9 annotated proteins occurred in binding, catalytic activity and cellular process whereas, 29 (70.73%) out of the 41 proteins had binding activity and 20 proteins (48.78%) had catalytic activity. The findings provided an insight into the interactive relationship between E. acervulina and host cells and will shed new lights on the understanding of molecular mechanisms of E. acervulina invasion and pathogenesis.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: ZhenChao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: JingWei
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: LianRui
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: MingMin
authors.initials: M
authors.lastname: Lu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: MengHui
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: YuXia
authors.initials: Y
authors.lastname: Sui
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: LiXin
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: RuoFeng
authors.initials: R
authors.lastname: Yan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: XiaoKai
authors.initials: X
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: lixiangrui@njau.edu.cn.
authors.forename: XiangRui
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods; D004386:Duodenum / Q000166:cytology / Q000469:parasitology; D004539:Eimeria / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism / Q000469:parasitology; D057131:Immune Evasion; D040901:Proteomics; D015800:Protozoan Proteins / Q000378:metabolism*; D034101:Sporozoites / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D015800:Protozoan Proteins
keywords: Chicken; Duodenal epithelial cells; E. acervulina; Sporozoite