title: Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae)(1).
abstract: OBJECTIVE
  Fifty candidate microsatellite markers, generated using 454 shotgun sequencing, were tested for the widespread arctic/alpine herb Silene acaulis (Caryophyllaceae).
  
  
  RESULTS
  Fourteen out of 50 markers resulted in polymorphic products with profiles that enabled interpretation. The numbers of alleles per locus ranged from two to six, and the expected heterozygosity per locus ranged from 0.06 to 0.68. Analysis of F0 and F1 samples proved that one allele was always inherited maternally. Four multiplex mixes have been developed.
  
  
  CONCLUSIONS
  Microsatellite markers for this species will be a valuable tool to study detailed small-scale genetic patterns in an arctic/alpine herb and to relate them to demographic parameters.
authors.affiliation: University Centre in Svalbard, Postboks 156, NO-9170 Longyearbyen, Norway.
authors.forename: Eike
authors.initials: E
authors.lastname: Müller
authors.affiliation: Institute of Chemical Technology, University of Chemistry and Technology (UCT) Prague, Technická 5, 16628 Prague, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Hlaváčková
authors.affiliation: University Centre in Svalbard, Postboks 156, NO-9170 Longyearbyen, Norway ; University of Oslo, Postboks 1072 Blindern, NO-0316 Oslo, Norway.
authors.forename: Mildrid Elvik
authors.initials: ME
authors.lastname: Svoen
authors.affiliation: University Centre in Svalbard, Postboks 156, NO-9170 Longyearbyen, Norway ; Tromsø Museum, University of Tromsø, Postboks 6050 Langnes, NO-9037 Tromsø, Norway.
authors.forename: Inger Greve
authors.initials: IG
authors.lastname: Alsos
authors.affiliation: University Centre in Svalbard, Postboks 156, NO-9170 Longyearbyen, Norway.
authors.forename: Pernille Bronken
authors.initials: PB
authors.lastname: Eidesen
keywords: Arctic/alpine; Caryophyllaceae; Silene acaulis; gynodioecious; moss campion