title: Sinorhizobium meliloti Controls Nitric Oxide-Mediated Post-Translational Modification of a Medicago truncatula Nodule Protein.
abstract: Nitric oxide (NO) is involved in various plant-microbe interactions. In the symbiosis between soil bacterium Sinorhizobium meliloti and model legume Medicago truncatula, NO is required for an optimal establishment of the interaction but is also a signal for nodule senescence. Little is known about the molecular mechanisms responsible for NO effects in the legume-rhizobium interaction. Here, we investigate the contribution of the bacterial NO response to the modulation of a plant protein post-translational modification in nitrogen-fixing nodules. We made use of different bacterial mutants to finely modulate NO levels inside M. truncatula root nodules and to examine the consequence on tyrosine nitration of the plant glutamine synthetase, a protein responsible for assimilation of the ammonia released by nitrogen fixation. Our results reveal that S. meliloti possesses several proteins that limit inactivation of plant enzyme activity via NO-mediated post-translational modifications. This is the first demonstration that rhizobia can impact the course of nitrogen fixation by modulating the activity of a plant protein.
authors.affiliation: 1 Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, UMR441, Castanet-Tolosan, France;|2 Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, UMR2594, Castanet-Tolosan, France; and.
authors.forename: Pauline
authors.initials: P
authors.lastname: Blanquet
authors.affiliation: 3 Laboratório de Biologia Molecular da Assimilação do Azoto, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal.
authors.forename: Liliana
authors.initials: L
authors.lastname: Silva
authors.affiliation: 1 Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, UMR441, Castanet-Tolosan, France;|2 Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, UMR2594, Castanet-Tolosan, France; and.
authors.forename: Olivier
authors.initials: O
authors.lastname: Catrice
authors.affiliation: 1 Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, UMR441, Castanet-Tolosan, France;|2 Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, UMR2594, Castanet-Tolosan, France; and.
authors.forename: Claude
authors.initials: C
authors.lastname: Bruand
authors.affiliation: 3 Laboratório de Biologia Molecular da Assimilação do Azoto, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal.
authors.forename: Helena
authors.initials: H
authors.lastname: Carvalho
authors.affiliation: 1 Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, UMR441, Castanet-Tolosan, France;|2 Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, UMR2594, Castanet-Tolosan, France; and.
authors.forename: Eliane
authors.initials: E
authors.lastname: Meilhoc
mesh_terms: D046913:Medicago truncatula; D009154:Mutation; D009569:Nitric Oxide / Q000502:physiology*; D010940:Plant Proteins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000502:physiology*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D009569:Nitric Oxide; D014443:Tyrosine