title: Biodistribution and Pharmacokinetic Study of 3,3' Diseleno Dipropionic Acid (DSePA), A Synthetic Radioprotector, in Mice.
abstract: OBJECTIVE
  3,3' Diseleno dipropionic acid (DSePA), a synthetic compound has been shown to have radioprotective activity, especially as a lung radioprotector. In this study, the pharmacokinetics and biodistribution of DSePA in MX-1 tumour bearing SCID mice were evaluated.
  
  
  METHODS
  Twenty SCID mice were administered DSePA (50 mg/kg bodyweight) by oral gavage following which four animals each were sacrificed at 15, 30 min, 1, 2 and 4 h. Blood and tissue samples were collected for determination of DSePA concentration by graphite furnace atomic absorption spectrometry (GFAAS) method. The control group (n = 4) was administered sterile water and sacrificed at 4 h.
  
  
  RESULTS
  Peak plasma concentration (Cmax) of 2.7 µg/ml was observed at 15 min which returned to near baseline (baseline = 0.6 µg/ml) at 1 h following drug administration. Biphasic pharmacokinetics characterized by rapid distribution phase and a slower elimination phase were observed. Highest maximal concentration (Cmax) of the drug was observed in lung (19.2 µg/g at 30 min) followed by intestine (14.64 µg/g at 15 min) and kidney (12.96 µg/g at 15 min). There was negligible uptake in tumor tissue and no uptake in brain.
  
  
  CONCLUSIONS
  DSePA has a favorable pharmacokinetic profile which makes it a potentially good candidate for further development as a radioprotective agent.
authors.affiliation: Department of Clinical Pharmacology, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, 410210, India. vgota76@gmail.com.
authors.forename: Vikram
authors.initials: V
authors.lastname: Gota
authors.affiliation: Department of Radiation Oncology, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, India.
authors.forename: Jayant S
authors.initials: JS
authors.lastname: Goda
authors.affiliation: Department of Clinical Pharmacology, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, 410210, India.
authors.forename: Kartik
authors.initials: K
authors.lastname: Doshi
authors.affiliation: Department of Clinical Pharmacology, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, 410210, India.
authors.forename: Anand
authors.initials: A
authors.lastname: Patil
authors.affiliation: Analytical Chemistry Division, Bhabha Atomic Research Centre, Mumbai, India.
authors.forename: Suvarna
authors.initials: S
authors.lastname: Sunderajan
authors.affiliation: Analytical Chemistry Division, Bhabha Atomic Research Centre, Mumbai, India.
authors.forename: Kiran
authors.initials: K
authors.lastname: Kumar
authors.affiliation: Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, India.
authors.forename: Mahendra
authors.initials: M
authors.lastname: Varne
authors.affiliation: Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kunwar
authors.affiliation: Chemistry Division, Bhabha Atomic Research Centre, Mumbai, India.
authors.forename: Vimal Kumar
authors.initials: VK
authors.lastname: Jain
authors.affiliation: Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, India.
authors.forename: Indira
authors.initials: I
authors.lastname: Priyadarshini
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D001812:Blood-Brain Barrier / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics*; D005260:Female; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics*; D006207:Half-Life; D006801:Humans; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008657:Metabolic Clearance Rate; D016513:Mice, SCID; D009928:Organ Specificity; D011422:Propionates / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics*; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics*; D018036:Selenium Compounds / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics*; D013054:Spectrophotometry, Atomic; D014018:Tissue Distribution; D047368:Tumor Burden; D023041:Xenograft Model Antitumor Assays
chemical_list: C524945:3,3'-diselenodipropionic acid; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D011422:Propionates; D011837:Radiation-Protective Agents; D018036:Selenium Compounds