title: Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples.
abstract: A polymerase chain reaction (PCR) buffer was systematically designed to relieve the inhibition caused by hydrophobic magnetic ionic liquids (MILs). We describe a simple, rapid method for MIL-based plasmid DNA extraction from crude bacterial cell lysate in which DNA-enriched MIL is transferred directly to a PCR tube for analysis.
authors.affiliation: Department of Chemistry, Iowa State University, Ames, IA 50011, USA. andersoj@iastate.edu.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Clark
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Yamsek
authors.forename: Omprakash
authors.initials: O
authors.lastname: Nacham
authors.forename: Jared L
authors.initials: JL
authors.lastname: Anderson
mesh_terms: D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques; D052578:Ionic Liquids / Q000737:chemistry*; D008280:Magnetics; D015394:Molecular Structure; D016133:Polymerase Chain Reaction; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
chemical_list: D052578:Ionic Liquids; D012997:Solvents; D004247:DNA; D011683:Purine-Nucleoside Phosphorylase; C021784:5'-methylthioadenosine phosphorylase