title: Characterization and comparison of polysaccharides from Lycium barbarum in China using saccharide mapping based on PACE and HPTLC.
abstract: Water-soluble polysaccharides from 51 batches of fruits of L. barbarum (wolfberry) in China were investigated and compared using saccharide mapping, partial acid hydrolysis, single and composite enzymatic digestion, followed by polysaccharide analysis by using carbohydrate gel electrophoresis (PACE) analysis and high performance thin layer chromatography (HPTLC) analysis, respectively. Results showed that multiple PACE and HPTLC fingerprints of partial acid and enzymatic hydrolysates of polysaccharides from L. barbarum in China were similar, respectively. In addition, results indicated that β-1,3-glucosidic, α-1,4-galactosiduronic and α-1,5-arabinosidic linkages existed in polysaccharides from L. barbarum collected in China, and the similarity of polysaccharides in L. barbarum collected from different regions of China was pretty high, which are helpful for the improvement of the performance of polysaccharides from L. barbarum in functional/health foods area. Furthermore, polysaccharides from Panax notoginseng, Angelica sinensis, and Astragalus membranaceus var. mongholicus were successfully distinguished from those of L. barbarum based on their PACE fingerprints. These results were beneficial to improve the quality control of polysaccharides from L. barabrum and their products, which suggested that saccharide mapping based on PACE and HPTLC analysis could be a routine approach for quality control of polysaccharides.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors.forename: Ding-Tao
authors.initials: DT
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors.forename: Kit-Leong
authors.initials: KL
authors.lastname: Cheong
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Deng
authors.affiliation: College of Chemistry and Life Sciences, Qinghai University for Nationalities, Xining 810007, China.
authors.forename: Peng-Cheng
authors.initials: PC
authors.lastname: Lin
authors.affiliation: National Institutes for Food and Drug Control, Tiantan Xili 2, Beijing 100050, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.affiliation: Inner Mongolia Medical University, Hohhot 010000, China.
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Lv
authors.affiliation: Xinjiang Uygur Autonomous Region Product Quality Supervision and Inspection Institute, Urumqi 830011, China.
authors.forename: Ze-Rong
authors.initials: ZR
authors.lastname: Long
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China. Electronic address: jingzhao@umac.mo.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China; National Institutes for Food and Drug Control, Tiantan Xili 2, Beijing 100050, China. Electronic address: masc@nifdc.org.cn.
authors.forename: Shuang-Cheng
authors.initials: SC
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China. Electronic address: spli@umac.mo.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Li
mesh_terms: D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D032305:Lycium / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D011134:Polysaccharides
keywords: HPTLC; Lycium barbarum; PACE; Polysaccharide; Saccharide mapping; Wolfberry