title: Development of a Highly Specific Fluorescence Immunoassay for Detection of Diisobutyl Phthalate in Edible Oil Samples.
abstract: The diisobutyl phthalate (DiBP) hapten containing an amino group was synthesized successfully, and the polyclonal antibody against 4-amino phthalate-bovine serum albumin (BSA) was developed. On the basis of the polyclonal antibody, a rapid and sensitive indirect competitive fluorescence immunoassay (icFIA) has been established to detect DiBP in edible oil samples for the first time. Under the optimized conditions, the quantitative working range of the icFIA was from 10.47 to 357.06 ng/mL (R(2) = 0.991), exhibiting a detection limit of 5.82 ng/mL. In this assay, the specific results showed that other similar phthalates did not significantly interfere with the analysis, with the cross-reactivity less than 1.5%, except for that of DiBAP. Thereafter, DiBP contamination in edible oil samples was detected by icFIA, with the recovery being from 79 to 103%. Furthermore, the reliability of icFIA was validated by gas chromatography-mass spectrometry (GC-MS). Therefore, the developed icFIA is suitable for monitoring DiBP in some edible oil samples.
authors.affiliation: Department of Pharmaceutical Engineering, Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology , Guangzhou, Guangdong 510006, People's Republic of China.
authors.forename: Xiping
authors.initials: X
authors.lastname: Cui
authors.affiliation: Department of Pharmaceutical Engineering, Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology , Guangzhou, Guangdong 510006, People's Republic of China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Wu
authors.affiliation: Department of Pharmaceutical Engineering, Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology , Guangzhou, Guangdong 510006, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Lai
authors.affiliation: Department of Pharmaceutical Engineering, Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology , Guangzhou, Guangdong 510006, People's Republic of China.
authors.forename: Shengwu
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Pharmaceutical Engineering, Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology , Guangzhou, Guangdong 510006, People's Republic of China.
authors.forename: Yingshan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov State University , Moscow 119992, Russia.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Eremin
authors.affiliation: School of Public Health, Guangzhou Medical University , Guangzhou, Guangdong 510006, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.affiliation: Department of Pharmaceutical Engineering, Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology , Guangzhou, Guangdong 510006, People's Republic of China.
authors.forename: Suqing
authors.initials: S
authors.lastname: Zhao
mesh_terms: D003993:Dibutyl Phthalate / Q000031:analogs & derivatives* / Q000032:analysis; D005453:Fluorescence; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D010938:Plant Oils / Q000737:chemistry*
chemical_list: D010938:Plant Oils; D003993:Dibutyl Phthalate; C025605:diisobutyl phthalate
keywords: diisobutyl phthalate; edible oil; fluorescence immunoassay; phthalate esters