title: Retrovirus-based vectors for transient and permanent cell modification.
abstract: Retroviral vectors are commonly employed for long-term transgene expression via integrating vector technology. However, three alternative retrovirus-based platforms are currently available that allow transient cell modification. Gene expression can be mediated from either episomal DNA or RNA templates, or selected proteins can be directly transferred through retroviral nanoparticles. The different technologies are functionally graded with respect to safety, expression magnitude and expression duration. Improvement of the initial technologies, including modification of vector designs, targeted increase in expression strength and duration as well as improved safety characteristics, has allowed maturation of retroviral systems into efficient and promising tools that meet the technological demands of a wide variety of potential application areas.
authors.affiliation: Institute of Experimental Hematology, Hannover Medical School, Hannover, Germany; Cluster of Excellence REBIRTH, Hannover Medical School, Hannover, Germany.
authors.forename: Juliane W
authors.initials: JW
authors.lastname: Schott
authors.affiliation: Institute of Experimental Hematology, Hannover Medical School, Hannover, Germany; Cluster of Excellence REBIRTH, Hannover Medical School, Hannover, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Hoffmann
authors.affiliation: Institute of Experimental Hematology, Hannover Medical School, Hannover, Germany; Cluster of Excellence REBIRTH, Hannover Medical School, Hannover, Germany; Division of Hematology/Oncology, Boston Children's Hospital, Harvard Medical School, Boston, USA. Electronic address: Schambach.Axel@mh-hannover.de.
authors.forename: Axel
authors.initials: A
authors.lastname: Schambach
mesh_terms: D000818:Animals; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids; D011506:Proteins / Q000008:administration & dosage; D012333:RNA, Messenger / Q000008:administration & dosage; D012190:Retroviridae / Q000235:genetics*
chemical_list: D011506:Proteins; D012333:RNA, Messenger