title: Identification of a characteristic antioxidant, anthrasesamone F, in black sesame seeds and its accumulation at different seed developmental stages.
abstract: Assay-guided fractionation of the methanol extract from black seeds of sesame (Sesamum indicum L.) led to the isolation of an active compound that had a 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. This antioxidant was confirmed to be anthrasesamone F, an anthraquinone derivative previously isolated from different black sesame seeds and biogenetically related to other anthrasesamones in sesame roots. The radical scavenging assay showed that anthrasesamone F had more potent activity than Trolox. The content of anthrasesamone F in different parts and at different developmental stages of black sesame seeds was investigated to clarify the accumulation pattern of this antioxidant in the black seeds. Anthrasesamone F was localized in the seed coat of black seeds and accumulated after the seed coat color changed to black. The content of anthrasesamone F increased gradually with seed maturation and drastically on air-drying, the final stage in sesame cultivation.
authors.affiliation: a Faculty of Agriculture, Department of Applied Biological Science , Kagawa University , Kagawa , Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Furumoto
authors.affiliation: a Faculty of Agriculture, Department of Applied Biological Science , Kagawa University , Kagawa , Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Nishimoto
mesh_terms: D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000737:chemistry; D002839:Chromans / Q000737:chemistry; D003116:Color; D010851:Picrates / Q000037:antagonists & inhibitors / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D012639:Seeds / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D031685:Sesamum / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000880:Anthraquinones; D000975:Antioxidants; D001713:Biphenyl Compounds; D002839:Chromans; D010851:Picrates; D010936:Plant Extracts; C530628:anthrasesamone F; C004931:1,1-diphenyl-2-picrylhydrazyl; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
keywords: Sesamum indicum; anthrasesamone F; antioxidant; black sesame seed; seed maturity