title: Early, Real-Time Medical Diagnosis of Botulism by Endopeptidase-Mass Spectrometry.
abstract: Botulinum toxin was detected in patient serum using Endopeptidase-mass-spectrometry assay, although all conventional tests provided negative results. Antitoxin was administered, resulting in patient improvement. Implementing this highly sensitive and rapid assay will improve preparedness for foodborne botulism and deliberate exposure.
authors.affiliation: Department of Biotechnology.
authors.forename: Osnat
authors.initials: O
authors.lastname: Rosen
authors.affiliation: Department of Analytical Chemistry.
authors.forename: Liron
authors.initials: L
authors.lastname: Feldberg
authors.affiliation: Department of Analytical Chemistry.
authors.forename: Sigalit
authors.initials: S
authors.lastname: Gura
authors.affiliation: CBRN Medicine Branch, Medical Corps HQ, Israel Institute for Biological Research, Ness Ziona.
authors.forename: Tal
authors.initials: T
authors.lastname: Brosh-Nissimov
authors.affiliation: Infectious Diseases Unit.
authors.forename: Alex
authors.initials: A
authors.lastname: Guri
authors.affiliation: Infectious Diseases Unit.
authors.forename: Oren
authors.initials: O
authors.lastname: Zimhony
authors.affiliation: Pediatric Intensive Care Unit, Kaplan Medical Center, Rehovot.
authors.forename: Eli
authors.initials: E
authors.lastname: Shapiro
authors.affiliation: Department of Biochemistry and Molecular Genetics.
authors.forename: Adi
authors.initials: A
authors.lastname: Beth-Din
authors.affiliation: Department of Biochemistry and Molecular Genetics.
authors.forename: Dana
authors.initials: D
authors.lastname: Stein
authors.affiliation: Department of Biotechnology.
authors.forename: Eyal
authors.initials: E
authors.lastname: Ozeri
authors.affiliation: Department of Biotechnology.
authors.forename: Ada
authors.initials: A
authors.lastname: Barnea
authors.affiliation: Department of Biotechnology.
authors.forename: Amram
authors.initials: A
authors.lastname: Turgeman
authors.affiliation: Department of Biotechnology.
authors.forename: Alon
authors.initials: A
authors.lastname: Ben David
authors.affiliation: Department of Biotechnology.
authors.forename: Arieh
authors.initials: A
authors.lastname: Schwartz
authors.affiliation: Department of Biochemistry and Molecular Genetics.
authors.forename: Eytan
authors.initials: E
authors.lastname: Elhanany
authors.affiliation: Department of Biotechnology.
authors.forename: Eran
authors.initials: E
authors.lastname: Diamant
authors.affiliation: Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.
authors.forename: Shmuel
authors.initials: S
authors.lastname: Yitzhaki
authors.affiliation: Department of Biotechnology.
authors.forename: Ran
authors.initials: R
authors.lastname: Zichel
mesh_terms: D000992:Antitoxins / Q000008:administration & dosage; D001906:Botulism / Q000175:diagnosis* / Q000628:therapy; D042241:Early Diagnosis; D010450:Endopeptidases / Q000097:blood*; D006801:Humans; D007223:Infant; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D044967:Serum / Q000737:chemistry; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D000992:Antitoxins; D010450:Endopeptidases
keywords: Endopep-MS; botulism; diagnosis