title: Mycobacterium angelicum sp. nov., a non-chromogenic, slow-growing species isolated from fish and related to Mycobacterium szulgai.
abstract: The name 'Mycobacterium angelicum' dates back to 2003 when it was suggested for a slowly growing mycobacterium isolated from freshwater angelfish. This name is revived here and the novel species is proposed on the basis of the polyphasic characterization of four strains including the original one. The four strains presented 100 % 16S rRNA gene sequence similarity with Mycobacterium szulgai but clearly differed from M. szulgai for the milky white aspect of the colonies. The sequence similarity with the type strain of M. szulgai ranged, in eight additionally investigated genetic targets, from 78.9 to 94.3 %, an evident contrast with the close relatedness that emerged at the level of 16S rRNA gene. The average nucleotide identity between the genomes of M. szulgai DSM 44166T and strain 126/5/03T (type strain of the novel species) was 92.92 %, and supported the status of independent species. The confirmation of the name Mycobacterium angelicum sp. nov. is proposed, with strain 126/5/03T ( = CIP 109313T = DSM 45057T) as the type strain.
authors.affiliation: School of Veterinary Medicine, Ilam University, Ilam, Iran.
authors.forename: Fazel
authors.initials: F
authors.lastname: Pourahmad
authors.affiliation: National Veterinary Institute, University of Ljubljana, Ljubljana, Slovenia.
authors.forename: Mateja
authors.initials: M
authors.lastname: Pate
authors.affiliation: National Veterinary Institute, University of Ljubljana, Ljubljana, Slovenia.
authors.forename: Matjaž
authors.initials: M
authors.lastname: Ocepek
authors.affiliation: Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Borroni
authors.affiliation: Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Cabibbe
authors.affiliation: Center for Translational Genomics and Bioinformatics, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Capitolo
authors.affiliation: Center for Translational Genomics and Bioinformatics, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Cittaro
authors.affiliation: Microbiology Unit, General Hospital, Bolzano-Bozen, Italy.
authors.forename: Eliana
authors.initials: E
authors.lastname: Frizzera
authors.affiliation: National Veterinary Institute, University of Ljubljana, Ljubljana, Slovenia.
authors.forename: Vlasta
authors.initials: V
authors.lastname: Jenčič
authors.affiliation: Diagnostic Genetics Unit, Careggi University Hospital, Florence, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Mariottini
authors.affiliation: Department of Microbiology and Immunology, Showa University, Tokyo, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Marumo
authors.affiliation: Mycrobiology and Virology Unit, Careggi University Hospital, Florence, Italy.
authors.forename: Guendalina
authors.initials: G
authors.lastname: Vaggelli
authors.affiliation: Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Cirillo
authors.affiliation: Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Tortoli
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D023681:Cichlids / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005618:Fresh Water; D007564:Japan; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009170:Nontuberculous Mycobacteria; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S