title: A Failure to Communicate: MYOSIN RESIDUES INVOLVED IN HYPERTROPHIC CARDIOMYOPATHY AFFECT INTER-DOMAIN INTERACTION.
abstract: Our molecular modeling studies suggest a charge-dependent interaction between residues Glu-497 in the relay domain and Arg-712 in the converter domain of human β-cardiac myosin. To test the significance of this putative interaction, we generated transgenic Drosophila expressing indirect flight muscle myosin with charge reversal mutations in the relay (E496R) or converter (R713E). Each mutation yielded dramatic reductions in myosin Ca-ATPase activity (~80%) as well as in basal (~67%) and actin-activated (~84%) Mg-ATPase activity. E496R myosin-induced in vitro actin-sliding velocity was reduced by 71% and R713E myosin permitted no actin motility. Indirect flight muscles of late pupae from each mutant displayed disrupted myofibril assembly, with adults having severely abnormal myofibrils and no flight ability. To understand the molecular basis of these defects, we constructed a putative compensatory mutant that expresses myosin with both E496R and R713E. Intriguingly, ATPase values were restored to ~73% of wild-type and actin-sliding velocity increased to 40%. The double mutation suppresses myofibril assembly defects in pupal indirect flight muscles and dramatically reduces myofibril disruption in young adults. Although sarcomere organization is not sustained in older flies and flight ability is not restored in homozygotes, young heterozygotes fly well. Our results indicate that this charge-dependent interaction between the myosin relay and converter domains is essential to the mechanochemical cycle and sarcomere assembly. Furthermore, the same inter-domain interaction is disrupted when modeling human β-cardiac myosin heavy chain cardiomyopathy mutations E497D or R712L, implying that abolishing this salt bridge is one cause of the human disease.
authors.affiliation: From the Department of Biology, Molecular Biology Institute and Heart Institute San Diego State University, San Diego, California 92182-4614.
authors.forename: William A
authors.initials: WA
authors.lastname: Kronert
authors.affiliation: From the Department of Biology, Molecular Biology Institute and Heart Institute San Diego State University, San Diego, California 92182-4614.
authors.forename: Girish C
authors.initials: GC
authors.lastname: Melkani
authors.affiliation: From the Department of Biology, Molecular Biology Institute and Heart Institute San Diego State University, San Diego, California 92182-4614.
authors.forename: Anju
authors.initials: A
authors.lastname: Melkani
authors.affiliation: From the Department of Biology, Molecular Biology Institute and Heart Institute San Diego State University, San Diego, California 92182-4614 sbernstein@mail.sdsu.edu.
authors.forename: Sanford I
authors.identifier: http://orcid.org/0000-0001-7094-5390
authors.initials: SI
authors.lastname: Bernstein
mesh_terms: D000199:Actins / Q000737:chemistry; D000251:Adenosine Triphosphatases / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D030801:Animals, Genetically Modified; D024722:Cardiac Myosins / Q000737:chemistry*; D002312:Cardiomyopathy, Hypertrophic / Q000503:physiopathology*; D002645:Chickens; D003433:Crosses, Genetic; D004195:Disease Models, Animal; D004331:Drosophila melanogaster; D005260:Female; D006801:Humans; D008958:Models, Molecular; D018482:Muscle, Skeletal / Q000378:metabolism; D009154:Mutation; D009210:Myofibrils / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000737:chemistry*; D049897:Pectinidae; D010641:Phenotype; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012492:Salts / Q000737:chemistry; D012518:Sarcomeres / Q000378:metabolism; D019076:Transgenes
chemical_list: D000199:Actins; D000596:Amino Acids; D012492:Salts; D000251:Adenosine Triphosphatases; D024722:Cardiac Myosins; D018995:Myosin Heavy Chains
keywords: ATPase; Drosophila; cardiomyopathy; electron microscopy; muscle; myofibril; myosin; suppressor mutation