title: Use of an IL1-receptor antagonist to prevent the progression of tendinopathy in a rat model.
abstract: This study evaluated if inhibiting IL1-β activity with an IL1-receptor antagonist (IL1-RA) will prevent pathologic changes commonly seen in tendinopathy. Thirty-six Sprague-Dawley retired-breeder rats were divided into three groups having weekly bilateral patellar tendon injections: CON (0.1 ml Saline), CAR (0.1 ml 2% carrageenan), IL1-RA (0.1 ml 2% CAR plus 0.94 mg of the IL1-RA, 2.5 mg/kg). Carrageenan was used to establish tendinopathy in two groups due to its ability to develop tendinopathy in prior studies. Animals were euthanized 3 weeks after initial injection. The CAR group demonstrated significantly (p < 0.05) shorter tendon lengths (8.61 ± 0.38 mm) relative to CON (8.94 ± 0.38 mm) that was prevented in the IL1-RA (9.02 ± 0.30 mm) as well as significantly increased collagenase activity in the CAR (0.061 ± 0.043) compared to CON (0.027 ± 0.015) (p<  0.05). By histological evaluation, the CAR group demonstrated significantly greater inflammation than IL1-RA, and CON (p < 0.05). CAR showed a trend for increased cross-sectional area relative to CON that was absent in the IL1-RA. IL1-RA can effectively inhibit the development of mechanical, chemical, and histologic changes seen with carrageenan-induced tendonitis.
authors.affiliation: Department of Orthopaedics, University of North Carolina-Chapel Hill, North Carolina UNC.
authors.forename: David J
authors.initials: DJ
authors.lastname: Berkoff
authors.affiliation: Department of Orthopaedics, University of North Carolina-Chapel Hill, North Carolina UNC.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Kallianos
authors.affiliation: Department of Orthopaedics, University of North Carolina-Chapel Hill, North Carolina UNC.
authors.forename: Scott M
authors.initials: SM
authors.lastname: Eskildsen
authors.affiliation: Department of Orthopaedics, University of North Carolina-Chapel Hill, North Carolina UNC.
authors.forename: Paul S
authors.initials: PS
authors.lastname: Weinhold
mesh_terms: D000818:Animals; D018501:Antirheumatic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002351:Carrageenan; D004195:Disease Models, Animal; D018450:Disease Progression; D004353:Drug Evaluation, Preclinical; D005260:Female; D053590:Interleukin 1 Receptor Antagonist Protein / Q000494:pharmacology / Q000627:therapeutic use*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D052256:Tendinopathy / Q000188:drug therapy*
chemical_list: D018501:Antirheumatic Agents; D053590:Interleukin 1 Receptor Antagonist Protein; D017472:Receptors, Interleukin-1; D002351:Carrageenan
keywords: IL1; carrageenan; inflammatory cytokine; patellar tendon; tendon inflammation