title: Effects of Cyanidin-3-O-glucoside on Synthetic and Metabolic Activity of Ethanol Stimulated Human Pancreatic Stellate Cells.
abstract: Activated pancreatic stellate cells (PSC) play a major role in the development of chronic pancreatitis. Flavonoids (C-3-O-G) theoretically may have potential to suppress activated PSC. The aim of our study was to determine the ability of C-3-O-G to invert synthetic and metabolic activity of alcohol stimulated human pancreatic stellate cells (hPSC). In the present study we demonstrate that treatment with C-3-O-G decreased proliferation rate of ethanol activated hPSC by 51%. Synthesis of extracellular matrix proteins in activated hPSC was markedly inhibited, as shown by reduced levels of collagen I and fibronectin expression. The decrease of secretion of fibronectin by 33% and in collagen I-25% in ethanol activated and C-3-O-G treated hPSC was observed. Moreover, treatment of ethanol activated hPSC with C-3-O-G resulted in the decrease of oxygen consumption rate by 44% and reduced levels of ATP synthesis (i.e. energy production) by 41%. Hence, the effects of C-3-O-G on ethanol activated hPSC may provide new insights for the use of anthocyanins as anti-fibrogenic agents in treatment and/or prevention of pancreatic fibrosis.
authors.affiliation: Department of Surgery, Lithuanian University of Health Sciences, Kaunas, Lithuania.|Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Vaidotas
authors.initials: V
authors.lastname: Cesna
authors.affiliation: Institute of Neurosciences, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Rasa
authors.initials: R
authors.lastname: Baniene
authors.affiliation: Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Aurelija
authors.initials: A
authors.lastname: Maziukiene
authors.affiliation: Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Kristina
authors.initials: K
authors.lastname: Kmieliute
authors.affiliation: Institute of Neurosciences, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Sonata
authors.initials: S
authors.lastname: Trumbeckaite
authors.affiliation: Department of Surgery, Lithuanian University of Health Sciences, Kaunas, Lithuania.|Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Linas
authors.initials: L
authors.lastname: Venclauskas
authors.affiliation: Department of Surgery, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Giedrius
authors.initials: G
authors.lastname: Barauskas
authors.affiliation: Department of Surgery, Lithuanian University of Health Sciences, Kaunas, Lithuania.|Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Antanas
authors.initials: A
authors.lastname: Gulbinas
mesh_terms: D000872:Anthocyanins / Q000494:pharmacology*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D005355:Fibrosis; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D058954:Pancreatic Stellate Cells / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D005960:Glucosides; C462279:cyanidin-3-O-beta-glucopyranoside; D000431:Ethanol
keywords: cyanidin-3-O-glucoside; energy metabolism; ethanol; pancreatic stellate cells