title: Phytomonospora cypria sp. nov., isolated from soil.
abstract: A Gram-stain positive actinobacterial strain, designated KT1403(T), was isolated from a soil sample, collected from Karpaz, Magusa, Northern Cyprus, and characterised using a polyphasic approach. Morphological characteristics and chemotaxonomic data indicated that the strain belongs to the genus Phytomonospora. The cell wall of the novel strain contained meso-diaminopimelic acid and galactose, glucose and mannose as the major sugars in whole cell hydrolysates. The polar lipids in the cell membrane were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified aminophospholipid and three unidentified glycolipids. The predominant menaquinones were MK-10(H6) and MK-10(H4). The major fatty acids were found to be iso C15:0 , anteiso C15:0 and anteiso C17:0. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KT1403(T) belongs to the genus Phytomonospora with a sequence similarity of 99.73 % with Phytomonospora endophytica, the type species of the genus. DNA-DNA hybridization further differentiated strain KT1403(T) from its near phylogenetic neighbour, P. endophytica DSM 45386(T) (29.0 ± 2.2 % DNA relatedness). Therefore, it is proposed that strain KT1403(T) represents a novel species of the genus Phytomonospora, for which the name Phytomonospora cypria sp. nov. is proposed. The type strain is KT1403(T) (=KCTC 29479(T) = DSM 46767(T)).
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139, Atakum-Samsun, Turkey. nsahin@omu.edu.tr.
authors.forename: Nevzat
authors.initials: N
authors.lastname: Sahin
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139, Atakum-Samsun, Turkey.|Department of Bioengineering, Faculty of Engineering and Architecture, Sinop University, 57000, Nasuhbasoglu-Sinop, Turkey.
authors.forename: Aysel
authors.initials: A
authors.lastname: Veyisoglu
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139, Atakum-Samsun, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Tatar
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139, Atakum-Samsun, Turkey.
authors.forename: Hayrettin
authors.initials: H
authors.lastname: Saygin
authors.affiliation: Science Teaching Programme, Gazi Faculty of Education, Gazi University, 06500, Ankara, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Cetin
authors.affiliation: Biology Department, Faculty of Science, Anadolu University, 26470, Eskisehir, Turkey.
authors.forename: Kiymet
authors.initials: K
authors.lastname: Guven
authors.affiliation: Department of Agricultural & Environmental Science, School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: Department of Agricultural & Environmental Science, School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Goodfellow
mesh_terms: D015373:Bacterial Typing Techniques; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D003535:Cyprus; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D008848:Micromonosporaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid
keywords: Micromonosporaceae; Northern Cyprus; Phytomonospora cypria; Polyphasic taxonomy