title: Effect of blue LED light intensity on carotenoid accumulation in citrus juice sacs.
abstract: In the present study, the effects of blue LED light intensity on carotenoid accumulation and expression of genes related to carotenoid biosynthesis were investigated in the juice sacs of Satsuma mandarin (Citrus unshiu Marc.) and Valencia orange (Citrus sinensis Osbeck) in vitro. The results showed that 100 μmol m(-2)s(-1) blue LED light (100B) was effective for increasing carotenoid content, especially β-cryptoxanthin, in Satsuma mandarin after cultured in vitro for four weeks. In Valencia orange, in contrast, 50 μmol m(-2)s(-1) blue LED light (50B) treatment was effective for inducing carotenoid accumulation through increasing the contents of two major carotenoids, all-trans-violaxanthin and 9-cis-violaxanthin. In addition, gene expression results showed that the simultaneous increases in the expression of genes (CitPSY, CitPDS, CitZDS, CitLCYb2, and CitHYb) involved in producing β,β-xanthophylls were well consistent with the accumulation of β-cryptoxanthin in Satsuma mandarin under 100B, and violaxanthin in Valencia orange under 50B. The results presented herein contribute to further elucidating the regulatory mechanism of carotenoid accumulation by blue LED light.
authors.affiliation: Department of Biological and Environmental Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga, Shizuoka 422-8529, Japan.
authors.forename: Lancui
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Biological and Environmental Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga, Shizuoka 422-8529, Japan.
authors.forename: Gang
authors.initials: G
authors.lastname: Ma
authors.affiliation: Department of Biological and Environmental Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga, Shizuoka 422-8529, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Yamawaki
authors.affiliation: Department of Citrus Research, NARO Institute of Fruit Tree Science, Okitsunakacho, Shimizu, Shizuoka 424-0292, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ikoma
authors.affiliation: Department of Citrus Research, NARO Institute of Fruit Tree Science, Okitsunakacho, Shimizu, Shizuoka 424-0292, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Matsumoto
authors.affiliation: Department of Citrus Research, NARO Institute of Fruit Tree Science, Okitsunakacho, Shimizu, Shizuoka 424-0292, Japan.
authors.forename: Terutaka
authors.initials: T
authors.lastname: Yoshioka
authors.affiliation: Department of Citrus Research, NARO Institute of Fruit Tree Science, Okitsunakacho, Shimizu, Shizuoka 424-0292, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohta
authors.affiliation: Department of Biological and Environmental Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga, Shizuoka 422-8529, Japan. Electronic address: amkato@ipc.shizuoka.ac.jp.
authors.forename: Masaya
authors.initials: M
authors.lastname: Kato
mesh_terms: D002338:Carotenoids / Q000378:metabolism / Q000528:radiation effects*; D002957:Citrus / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects*; D005638:Fruit / Q000378:metabolism / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D008027:Light; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity
chemical_list: D010940:Plant Proteins; D002338:Carotenoids
keywords: Blue LED light; Carotenoid; Citrus; Juice sacs; Regulatory mechanism