title: [Use of mass spectrometry for rapid diagnosis of positive blood cultures: Validation of a local algorythm].
abstract: Mass spectrometry (MS) is used in identification of positive blood culture, a contribution in the clinical management of septic patients. The protocol is labor intensive and disrupts the normal workflow in a clinical laboratory. We intended to make rapid diagnosis by using MS directly from shortly incubated blood agar plates (4 to 6 hours) comparing with results of the conventional method (MC). We worked in parallel 145 positive blood cultures, with correct identification by short method in 79% of cases. We observed better yield with non carbon bottles and with gramnegative rods. With this information we designed a rapid identification algorithm using MS, which allows advancing diagnosis in 12-16 hours, without increasing to the costs or work load, since extraction protocol is not used.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Hervé
authors.forename: Valerie
authors.initials: V
authors.lastname: Corvalán
authors.forename: Sara
authors.initials: S
authors.lastname: Hormazábal
authors.forename: Alberto
authors.initials: A
authors.lastname: Salas
authors.forename: Catalina
authors.initials: C
authors.lastname: Cabezas
authors.forename: Nora
authors.initials: N
authors.lastname: Badilla
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Munizaga
authors.forename: Fabiola
authors.initials: F
authors.lastname: Quintana
authors.forename: Sebastián
authors.initials: S
authors.lastname: de la Fuente
mesh_terms: D000465:Algorithms; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology; D003470:Culture Media / Q000032:analysis; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests
chemical_list: D003470:Culture Media