title: 2D NMR Trace Analysis by Continuous Hyperpolarization at High Magnetic Field.
abstract: Nuclear magnetic resonance is often the technique of choice in chemical analysis because of its sensitivity to molecular structure, quantitative character, and straightforward sample preparation. However, determination of trace analytes in complex mixtures is generally limited by low sensitivity and extensive signal overlap. Here, we present an approach for continuous hyperpolarization at high magnetic field that is based on signal amplification by reversible exchange (SABRE) and can be straightforwardly incorporated in multidimensional NMR experiments. This method was implemented in a 2D correlation experiment that allows detection and quantification of analytes at nanomolar concentration in complex solutions.
authors.affiliation: Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, Nijmegen, 6525 AJ (Netherlands).
authors.forename: Nan
authors.initials: N
authors.lastname: Eshuis
authors.affiliation: Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, Nijmegen, 6525 AJ (Netherlands).
authors.forename: Ruud L E G
authors.initials: RL
authors.lastname: Aspers
authors.affiliation: Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, Nijmegen, 6525 AJ (Netherlands).
authors.forename: Bram J A
authors.initials: BJ
authors.lastname: van Weerdenburg
authors.affiliation: Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, Nijmegen, 6525 AJ (Netherlands).
authors.forename: Martin C
authors.initials: MC
authors.lastname: Feiters
authors.affiliation: Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, Nijmegen, 6525 AJ (Netherlands).
authors.forename: Floris P J T
authors.initials: FP
authors.lastname: Rutjes
authors.affiliation: Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, Nijmegen, 6525 AJ (Netherlands).
authors.forename: Sybren S
authors.initials: SS
authors.lastname: Wijmenga
authors.affiliation: Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, Nijmegen, 6525 AJ (Netherlands). m.tessari@science.ru.nl.
authors.forename: Marco
authors.initials: M
authors.lastname: Tessari
keywords: hyperpolarization; multidimensional NMR spectroscopy; para-hydrogen; signal amplification; trace analysis