title: Cytopathology of the trachea of Bombyx mori (Lepidoptera: Bombycidae) to Bombyx mori nucleopolyhedrovirus.
abstract: Bombyx mori is a holometabolous insect found only in germplasm banks and morphological data related of resistance and susceptibility to diseases is important when selecting hybrids for commercial and scientific interest. This study analyzed the cytopathology of B. mori trachea to BmNPV, isolated geographically in Paraná state, Brazil. Fifth instar larvae were divided into two groups, control and inoculated; the viral suspension used was 2.4×10(7) polyhedral occlusion bodies/mL. From the second to the ninth day post-inoculation, segments of silkworm organs, containing the trachea, were processed for transmission electron microscopy. Analyses of fresh hemolymph were also performed to verify the susceptibility of the hemocytes. Signs of infection were initially detected in the hemocytes and in the tracheal cells on the second and fourth post-inoculation days, respectively. The cytopathology of the trachea showed all stages of the viral cycle, which was the same as in other tissues. Virions were detected in the basal lamina, which showed structural disorganization. So, the infection time of the hemocytes prior to trachea and the presence of virus in basal lamina, suggests that the trachea was a secondary target, infected by budded virus coming from the hemolymph.
authors.affiliation: Laboratório de Biologia Celular, Mestrado de Biociências e Saúde, Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná 85819-110, Brazil.
authors.forename: Jéssica Vencatto
authors.initials: JV
authors.lastname: Senem
authors.affiliation: Laboratório de Biologia Celular, Mestrado de Biociências e Saúde, Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná 85819-110, Brazil.
authors.forename: Ednéia Fátima Brambilla
authors.initials: EF
authors.lastname: Torquato
authors.affiliation: Laboratório de Biologia Celular, Mestrado de Biociências e Saúde, Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná 85819-110, Brazil.
authors.forename: Lucinéia de Fátima Chasko
authors.initials: Lde F
authors.lastname: Ribeiro
authors.affiliation: Laboratório de Biologia Celular, Mestrado de Biociências e Saúde, Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná 85819-110, Brazil. Electronic address: rosebombyx@gmail.com.
authors.forename: Rose Meire Costa
authors.initials: RM
authors.lastname: Brancalhão
mesh_terms: D000818:Animals; D012831:Bombyx / Q000821:virology*; D001938:Brazil; D046529:Microscopy, Electron, Transmission; D017924:Nucleopolyhedroviruses / Q000254:growth & development*; D014132:Trachea / Q000473:pathology / Q000821:virology
keywords: Baculoviridae; Lepidoptera; Tracheal cells