title: Extensive weight loss reveals distinct gene expression changes in human subcutaneous and visceral adipose tissue.
abstract: Weight loss has been shown to significantly improve Adipose tissue (AT) function, however changes in AT gene expression profiles particularly in visceral AT (VAT) have not been systematically studied. Here, we tested the hypothesis that extensive weight loss in response to bariatric surgery (BS) causes AT gene expression changes, which may affect energy and lipid metabolism, inflammation and secretory function of AT. We assessed gene expression changes by whole genome expression chips in AT samples obtained from six morbidly obese individuals, who underwent a two step BS strategy with sleeve gastrectomy as initial and a Roux-en-Y gastric bypass as second step surgery after 12 ± 2 months. Global gene expression differences in VAT and subcutaneous (S)AT were analyzed through the use of genome-scale metabolic model (GEM) for adipocytes. Significantly altered gene expressions were PCR-validated in 16 individuals, which also underwent a two-step surgery intervention. We found increased expression of cell death-inducing DFFA-like effector a (CIDEA), involved in formation of lipid droplets in both fat depots in response to significant weight loss. We observed that expression of the genes associated with metabolic reactions involved in NAD+, glutathione and branched chain amino acid metabolism are significantly increased in AT depots after surgery-induced weight loss.
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden.|Science for Life Laboratory, KTH - Royal Institute of Technology, SE-171 21, Stockholm, Sweden.
authors.forename: Adil
authors.initials: A
authors.lastname: Mardinoglu
authors.affiliation: University of Leipzig, Department of Medicine, Leipzig, Germany.
authors.forename: John T
authors.initials: JT
authors.lastname: Heiker
authors.affiliation: Städtisches Klinikum Karlsruhe, Clinic of Visceral Surgery, Karlsruhe, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gärtner
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden.
authors.forename: Elias
authors.initials: E
authors.lastname: Björnson
authors.affiliation: Städtisches Klinikum Karlsruhe, Clinic of Visceral Surgery, Karlsruhe, Germany.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Schön
authors.affiliation: University of Leipzig, Department of Medicine, Leipzig, Germany.
authors.forename: Gesine
authors.initials: G
authors.lastname: Flehmig
authors.affiliation: IFB Adiposity Diseases, Junior Research Group 2 "Animal models of obesity"
authors.forename: Nora
authors.initials: N
authors.lastname: Klöting
authors.affiliation: Core Unit DNA-Technologies, Interdisziplinäres Zentrum für Klinische Forschung (IZKF) Leipzig, Germany.
authors.forename: Knut
authors.initials: K
authors.lastname: Krohn
authors.affiliation: University of Leipzig, Department of Medicine, Leipzig, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Fasshauer
authors.affiliation: University of Leipzig, Department of Medicine, Leipzig, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Stumvoll
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden.|Science for Life Laboratory, KTH - Royal Institute of Technology, SE-171 21, Stockholm, Sweden.
authors.forename: Jens
authors.initials: J
authors.lastname: Nielsen
authors.affiliation: University of Leipzig, Department of Medicine, Leipzig, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Blüher
mesh_terms: D000328:Adult; D015992:Body Mass Index; D005260:Female; D015390:Gastric Bypass; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D066292:Lipid Droplets / Q000378:metabolism; D008297:Male; D053858:Metabolic Networks and Pathways; D008875:Middle Aged; D009767:Obesity, Morbid / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D050507:Subcutaneous Fat, Abdominal / Q000378:metabolism*; D059467:Transcriptome; D015431:Weight Loss