title: Analysis of watermelon chlorotic stunt virus and tomato leaf curl Palampur virus mixed and pseudo-recombination infections.
abstract: Watermelon chlorotic stunt virus (WmCSV) and tomato leaf curl Palampur virus (ToLCPMV) are limiting factors for cucurbit production in south and southeastern Iran. ToLCPMV infects all cucurbit crops (except watermelons) whereas WmCSV is somewhat limited to watermelon, causing detrimental effects on fruit production. In a survey, we detected WmCSV in all watermelon growing farms in Fars province (southern Iran). Given that WmCSV and ToLCPMV are present in the same geographical location in Iran, we studied the interaction of two viruses. Co-infection using agroinfectious clones of WmCSV and ToLCPMV caused severe symptoms in watermelon and zucchini in comparison to symptoms observed from individual infections. Interestingly, inoculation of zucchini with WmCSV DNA-A and ToLCPMV DNA-B agroinfectious clones or vice versa produced a viable pseudo-recombinant and induced systemic symptoms. This demonstrates that replication-associated protein of DNA-A of each virus is able to bind to cis elements of the DNA-B molecules of another virus.
authors.affiliation: Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Esmaeili
authors.affiliation: Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran. jheydarnejad@uk.ac.ir.
authors.forename: Jahangir
authors.initials: J
authors.lastname: Heydarnejad
authors.affiliation: Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Hossain
authors.initials: H
authors.lastname: Massumi
authors.affiliation: Structural Biology Research Unit, Division of Medical Biochemistry, Department of Clinical Laboratory Sciences, University of Cape Town, Rondebosch, Cape Town, 7701, South Africa.|Department of Plant Pathology and Emerging Pathogens Institute, University of Florida, Gainesville, FL, 32611, USA.|School of Biological Sciences, and Biomolecular Interaction Centre, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.
authors.forename: Arvind
authors.initials: A
authors.lastname: Varsani
mesh_terms: D001483:Base Sequence; D053839:Begomovirus / Q000235:genetics* / Q000472:pathogenicity*; D029773:Citrullus; D060085:Coinfection / Q000821:virology; D028464:Cucurbita; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D016679:Genome, Viral; D007492:Iran; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D016415:Sequence Alignment
chemical_list: D004279:DNA, Viral
keywords: Begomovirus; Interaction; Iran; Pseudo-recombination; Tomato leaf curl Palampur virus; Watermelon chlorotic stunt virus