title: Analgesic and Anti-Inflammatory Properties of Gelsolin in Acetic Acid Induced Writhing, Tail Immersion and Carrageenan Induced Paw Edema in Mice.
abstract: Plasma gelsolin levels significantly decline in several disease conditions, since gelsolin gets scavenged when it depolymerizes and caps filamentous actin released in the circulation following tissue injury. It is well established that our body require/implement inflammatory and analgesic responses to protect against cell damage and injury to the tissue. This study was envisaged to examine analgesic and anti-inflammatory activity of exogenous gelsolin (8 mg/mouse) in mice models of pain and acute inflammation. Administration of gelsolin in acetic acid-induced writhing and tail immersion tests not only demonstrated a significant reduction in the number of acetic acid-induced writhing effects, but also exhibited an analgesic activity in tail immersion test in mice as compared to placebo treated mice. Additionally, anti-inflammatory function of gelsolin (8 mg/mouse) compared with anti-inflammatory drug diclofenac sodium (10 mg/kg)] was confirmed in the carrageenan injection induced paw edema where latter was measured by vernier caliper and fluorescent tomography imaging. Interestingly, results showed that plasma gelsolin was capable of reducing severity of inflammation in mice comparable to diclofenac sodium. Analysis of cytokines and histopathological examinations of tissue revealed administration of gelsolin and diclofenac sodium significantly reduced production of pro-inflammatory cytokines, TNF-α and IL-6. Additionally, carrageenan groups pretreated with diclofenac sodium or gelsolin showed a marked decrease in edema and infiltration of inflammatory cells in paw tissue. Our study provides evidence that administration of gelsolin can effectively reduce the pain and inflammation in mice model.
authors.affiliation: Division of Animal Facility, Council of Scientific and Industrial Research-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Gupta
authors.affiliation: Division of Animal Facility, Council of Scientific and Industrial Research-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Devraj
authors.initials: D
authors.lastname: Parasar
authors.affiliation: Division of Protein Science & Engineering, Council of Scientific and Industrial Research-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Amin
authors.initials: A
authors.lastname: Sagar
authors.affiliation: Division of Protein Science & Engineering, Council of Scientific and Industrial Research-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Choudhary
authors.affiliation: Division of Animal Facility, Council of Scientific and Industrial Research-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Bhupinder Singh
authors.initials: BS
authors.lastname: Chopra
authors.affiliation: Division of Protein Science & Engineering, Council of Scientific and Industrial Research-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Garg
authors.affiliation: Division of Protein Science & Engineering, Council of Scientific and Industrial Research-Institute of Microbial Technology, Chandigarh, India.
authors.lastname: Ashish
authors.affiliation: Division of Animal Facility, Council of Scientific and Industrial Research-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Khatri
mesh_terms: D019342:Acetic Acid / Q000009:adverse effects; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002351:Carrageenan / Q000378:metabolism*; D004008:Diclofenac / Q000494:pharmacology; D004195:Disease Models, Animal; D004487:Edema / Q000473:pathology*; D005121:Extremities / Q000473:pathology; D005260:Female; D018260:Gelsolin / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy*; D008807:Mice, Inbred BALB C; D013623:Tail / Q000187:drug effects
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D018260:Gelsolin; D004008:Diclofenac; D002351:Carrageenan; D019342:Acetic Acid