title: Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen.
abstract: To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%), whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer ELISA.
authors.affiliation: Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Bannai
authors.forename: Manabu
authors.initials: M
authors.lastname: Nemoto
authors.forename: Koji
authors.initials: K
authors.lastname: Tsujimura
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamanaka
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D060326:Chemistry Techniques, Synthetic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D023141:Herpesvirus 4, Equid / Q000302:isolation & purification*; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins / Q000138:chemical synthesis / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D010446:Peptide Fragments; D014759:Viral Envelope Proteins; C076629:glycoprotein G, Equid herpesvirus 4