title: Estradiol promotes cells invasion by activating β-catenin signaling pathway in endometriosis.
abstract: Endometriosis is an estrogen-dependent disease that involves the adhesion, invasion, and angiogenesis of endometrial tissues outside of the uterine cavity. We hypothesized that a link exists between estrogen and beta-catenin (β-catenin) signaling in the pathogenesis of endometriosis. Human endometrial stromal cells (HESCs) were separated from eutopic endometrial tissues that were obtained from patients with endometriosis. β-catenin expression and cells invasiveness ability were up-regulated by 17β-estradiol (E2) in an estrogen receptor (ESR)-dependent manner, whereas β-catenin siRNA abrogated this phenomenon. Moreover, co-immunoprecipitation and dual immunofluorescence studies confirmed ESR1, β-catenin, and lymphoid enhancer factor 1/T cell factor 3 co-localization in the nucleus in HESCs after E2 treatment. To determine the role of β-catenin signaling in the implantation of ectopic endometrium, we xenotransplanted eutopic endometrium from endometriosis patients into ovariectomized severe combined immunodeficiency mice. The implantation of the endometrium was suppressed by β-catenin siRNA. Collectively, studies regarding β-catenin signaling are critical for improving our understanding of the pathogenesis of estrogen-induced endometriosis, which can translate into the development of treatments and therapeutic strategies for endometriosis.
authors.affiliation: Department of Obstetrics and GynecologyTongji Medical College, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Obstetrics and GynecologyUnion Hospital, Huazhong University of Science and Technology, 1277 JieFang Road, Wuhan, China.
authors.forename: Wenqian
authors.initials: W
authors.lastname: Xiong
authors.affiliation: Department of Obstetrics and GynecologyTongji Medical College, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Obstetrics and GynecologyUnion Hospital, Huazhong University of Science and Technology, 1277 JieFang Road, Wuhan, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and GynecologyTongji Medical College, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Obstetrics and GynecologyUnion Hospital, Huazhong University of Science and Technology, 1277 JieFang Road, Wuhan, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Obstetrics and GynecologyTongji Medical College, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Obstetrics and GynecologyUnion Hospital, Huazhong University of Science and Technology, 1277 JieFang Road, Wuhan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xie
authors.affiliation: Department of Obstetrics and GynecologyTongji Medical College, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Obstetrics and GynecologyUnion Hospital, Huazhong University of Science and Technology, 1277 JieFang Road, Wuhan, China.
authors.forename: Yicun
authors.initials: Y
authors.lastname: Man
authors.affiliation: Department of Obstetrics and GynecologyTongji Medical College, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Obstetrics and GynecologyUnion Hospital, Huazhong University of Science and Technology, 1277 JieFang Road, Wuhan, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Obstetrics and GynecologyTongji Medical College, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Obstetrics and GynecologyUnion Hospital, Huazhong University of Science and Technology, 1277 JieFang Road, Wuhan, China.
authors.forename: Hengwei
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and GynecologyTongji Medical College, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Obstetrics and GynecologyUnion Hospital, Huazhong University of Science and Technology, 1277 JieFang Road, Wuhan, China Department of Obstetrics and GynecologyTongji Medical College, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Obstetrics and GynecologyUnion Hospital, Huazhong University of Science and Technology, 1277 JieFang Road, Wuhan, China liqun94@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004715:Endometriosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000637:transplantation; D004958:Estradiol / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000378:metabolism; D005260:Female; D064593:Heterografts; D006801:Humans; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000378:metabolism; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010052:Ovariectomy; D034622:RNA Interference; D015398:Signal Transduction / Q000187:drug effects*; D017154:Stromal Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014162:Transfection; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004958:Estradiol; D047628:Estrogen Receptor alpha; D051821:Lymphoid Enhancer-Binding Factor 1; D051176:beta Catenin; C495270:CTNNB1 protein, human; C506487:ESR1 protein, human; C497573:LEF1 protein, human; C407909:TCF3 protein, human