title: Effect of Signal Intensity on Measurement of Ganglion Cell Complex and Retinal Nerve Fiber Layer Scans in Fourier-Domain Optical Coherence Tomography.
abstract: OBJECTIVE
  We determined the effect of Fourier-domain optical coherence tomography (OCT) signal strength index (SSI) and cropping on retinal nerve fiber layer (RNFL) and macular ganglion cell complex (GCC) scan repeatability and measurement thickness.
  
  
  METHODS
  Eyes were enrolled in the longitudinal Advanced Imaging for Glaucoma Study. At each visit, three repeat scans from the optic nerve head and macular protocols were obtained. Each measurement was associated with an SSI value from 0 to 100. Measurements with similar SSI scores were grouped to calculate repeatability defined as pooled standard deviation. Within-visit analysis was used to determine how measured thickness changed in relation to change in SSI level.
  
  
  RESULTS
  The study included 1130 eyes of 569 patients. Cropped images yielded significantly worse repeatability and they were excluded from subsequent analyses. The within-visit repeatability for RNFL and GCC measurements were significantly better with higher signal strength, and optimal cutoffs were SSI ≥ 37 and ≥ 44, respectively. The coefficient of variation was <1.8% for RNFL scans with SSI ≥ 37 and < 2% for GCC with SSI ≥ 44. For scans above the cutoff SSI, higher SSI's were correlated with thicker RNFL among normal (slope = 0.056 μm/SSI unit,P< 0.001) eyes and glaucoma suspect and perimetric glaucoma (GSPPG) eyes (slope = 0.060 μm/SSI unit,P< 0.001), but not for perimetric glaucoma (PG) eyes. No significant correlation was found for GCC.
  
  
  CONCLUSIONS
  Repeatability of RNFL and GCC thickness measurements may be improved by excluding images with cropped anatomic features and weak signal strength below recommended SSI cutoffs.
  
  
  CONCLUSIONS
  Measurement precision and image quality of inner eye structure by advanced imaging modality are important for clinical diagnosis and tracking of glaucoma disease.
authors.affiliation: Center for Ophthalmic Optics and Lasers, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States.
authors.forename: Xinbo
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami Miller School of Medicine, Palm Beach Gardens, Florida, United States.
authors.forename: Shawn M
authors.initials: SM
authors.lastname: Iverson
authors.affiliation: Center for Ophthalmic Optics and Lasers, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States.
authors.forename: Ou
authors.initials: O
authors.lastname: Tan
authors.affiliation: Center for Ophthalmic Optics and Lasers, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States.
authors.forename: David
authors.initials: D
authors.lastname: Huang
keywords: ganglion cell complex; glaucoma progression; optical coherence tomography; retinal imaging; retinal nerve fiber layer; signal strength