title: Comparison of Antimicrobial Activity between Chemical Disinfectants on Contaminated Orthodontic Pliers.
abstract: To compare the antimicrobial activity of the chemical substances--70% isopropyl alcohol, 2% glutaraldehyde (GTA) and 0.25% peracetic acid (PAA) in disinfecting orthodontic pliers contaminated in vitro with Streptococcui mutani, Staphylococci aureui and Candida albicani.
  
  
  
  Distal end cutter pliers were divided into five groups: group 1 (negative control--sterilized pliers), group 2 (positive control--sterilized plier, subsequently contaminated), group 3 (disinfected with 70% isopropyl alcohol, friction method), group 4 (disinfected with 2% GTA, immersion method for 30 minutes), group 5 (disinfected with 0.25% peracetic acid (PAA), immersion method for 10 minutes). After the pliers were treated with one disinfectant and submitted to microbiological evaluation (by counting colony forming units), they were submitted to the same cleansing, sterilizing and contaminating processes, and were used in the following groups (crossover and washout study). The two-factor analysis of variance (ANOVA) test, followed by the Tukey test, was used to compare the groups.
  
  
  
  The results showed that there was no statistically significant difference between the three tested disinfectants.
  
  
  
  Although there were no statistically significant differences between the disinfectants, the chemical agents 2% glutaraldehyde and 0.25% PAA were effective in inhibiting the growth of the three microorganisms tested; however, 70% isopropyl alcohol was unable to completely eliminate S. aureui.
  
  
  
  The chemical substances 2% glutaraldehyde and 0.25% PAA completely eliminated the microorganisms tested.
authors.affiliation: Department of Postgraduation in Dentistry, CEUMA University, Sao Luis, Maranhao, Brazil.
authors.forename: Maria Reggiani Azevedo
authors.initials: MR
authors.lastname: Carvalho
authors.affiliation: Department of Postgraduation in Dentistry, CEUMA University, Sao Luis, Maranhao, Brazil.
authors.forename: Marcos André
authors.initials: MA
authors.lastname: dos Santos da Silva
authors.affiliation: Department of Parasitology and Microbiology, Federal University of Piaui, Teresina, PI, Brazil.
authors.forename: Carla Adriana Rodrigues
authors.initials: CA
authors.lastname: de Sousa Brito
authors.affiliation: Department of Parasitology and Microbiology, Federal University of Piaui, Teresina, PI, Brazil.
authors.forename: Viriato
authors.initials: V
authors.lastname: Campelo
authors.affiliation: Department of Restorative Dentistry, Araraquara Dental School, University Estadual Paulista, Araraquara, Sao Paulo Maranhao, Brazil.
authors.forename: Milton Carlos
authors.initials: MC
authors.lastname: Kuga
authors.affiliation: Department of Postgraduate in Integrated Dental Science University of Cuiaba, Cuiaba, MT, Brazil.
authors.forename: Mateus Rodrigues
authors.initials: MR
authors.lastname: Tonetto
authors.affiliation: Department of Postgraduation in Dentistry, CEUMA University, Sao Luis, Maranhao, Brazil.
authors.forename: Rudys Rodolfo
authors.initials: RR
authors.lastname: De Jesus Tavarez
authors.affiliation: Professor, Department of Postgraduation in Dentistry, CEUMA University, Sao Luis, Maranhao, Brazil, e-mail: mbandeca@gmail.com.
authors.forename: Matheus Coelho
authors.initials: MC
authors.lastname: Bandéca
authors.affiliation: Department of Postgraduation in Dentistry, CEUMA University, Sao Luis, Maranhao, Brazil.
authors.forename: Celia Regina Maio
authors.initials: CR
authors.lastname: Pinzan-Vercelino
mesh_terms: D019840:2-Propanol / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects; D015169:Colony Count, Microbial; D003762:Dental Instruments / Q000382:microbiology*; D004203:Disinfection / Q000379:methods*; D004866:Equipment Contamination / Q000517:prevention & control*; D005976:Glutaral / Q000494:pharmacology; D006801:Humans; D009970:Orthodontics / Q000295:instrumentation*; D010463:Peracetic Acid / Q000494:pharmacology; D013291:Streptococcus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D010463:Peracetic Acid; D019840:2-Propanol; D005976:Glutaral
keywords: Disinfection; Glutaraldehyde; Infection control; Iso-propyl alcohol; Laboratory research; Microbiology; Orthodontics; Peracetic acid