title: Inhibition of aldose reductase and anti-cataract action of trans-anethole isolated from Foeniculum vulgare Mill. fruits.
abstract: Foeniculum vulgare fruits are routinely consumed for their carminative and mouth freshening effect. The plant was evaluated for aldose reductase inhibition and anti-diabetic action. Bioguided fractionation using silica gel column chromatography, HPLC, and GC-MS analysis revealed trans-anethole as the bioactive constituent possessing potent aldose reductase inhibitory action, with an IC50 value of 3.8μg/ml. Prolonged treatment with the pet ether fraction of the F. vulgare distillate demonstrated improvement in blood glucose, lipid profile, glycated haemoglobin and other parameters in streptozotocin-induced diabetic rats. Trans-anethole could effectively show anti-cataract activity through the increase in soluble lens protein, reduced glutathione, catalase and SOD activity on in vitro incubation of the eye lens with 55mM glucose. Trans-anethole demonstrated noncompetitive to mixed type of inhibition of lens aldose reductase using Lineweaver Burk plot.
authors.affiliation: Department of Biochemistry, Shivaji University, Kolhapur, Maharashtra 416004, India.
authors.forename: Vandana
authors.initials: V
authors.lastname: Dongare
authors.affiliation: Department of Biochemistry, Shivaji University, Kolhapur, Maharashtra 416004, India.
authors.forename: Chaitanya
authors.initials: C
authors.lastname: Kulkarni
authors.affiliation: Appasaheb Birnale College of Pharmacy, Sangli, Maharashtra 416416, India.
authors.forename: Manish
authors.initials: M
authors.lastname: Kondawar
authors.affiliation: Appasaheb Birnale College of Pharmacy, Sangli, Maharashtra 416416, India.
authors.forename: Chandrakant
authors.initials: C
authors.lastname: Magdum
authors.affiliation: Ayurveda Clinic, Kolhapur, Maharashtra 416008, India.
authors.forename: Vivek
authors.initials: V
authors.lastname: Haldavnekar
authors.affiliation: Department of Biochemistry, Shivaji University, Kolhapur, Maharashtra 416004, India. Electronic address: drauarvindekar@yahoo.co.in.
authors.forename: Akalpita
authors.initials: A
authors.lastname: Arvindekar
mesh_terms: D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000081248:Allylbenzene Derivatives; D000818:Animals; D000840:Anisoles / Q000737:chemistry*; D002386:Cataract / Q000517:prevention & control*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D030001:Foeniculum / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin / Q000009:adverse effects*
chemical_list: D000081248:Allylbenzene Derivatives; D000840:Anisoles; D010936:Plant Extracts; D013311:Streptozocin; D000449:Aldehyde Reductase; C006578:anethole
keywords: Aldose reductase inhibitor; Anti-cataract; Antidiabetic; Foeniculum vulgare; Trans-anethole