title: Changing in lipid profile induced by the mutation of Foxn1 gene: A lipidomic analysis of Nude mice skin.
abstract: Nude mice carry a spontaneous mutation affecting the gene Foxn1 mainly expressed in the epidermis. This gene is involved in several skin functions, especially in the proliferation and the differentiation of keratinocytes which are key cells of epithelial barrier. The skin, a protective barrier for the body, is essentially composed of lipids. Taking into account these factors, we conducted a lipidomic study to search for any changes in lipid composition of skin possibly related to Foxn1 mutation. Lipids were extracted from skin biopsies of Nude and BALB/c mice to be analyzed by liquid chromatography coupled to a high resolution mass spectrometer (HRMS). Multivariate and univariate data analyses were carried out to compare lipid extracts. Identification was performed using HRMS data, retention time and mass spectrometry fragmentation study. These results indicate that mutation of Foxn1 leads to significant modifications in the lipidome in Nude mice skin. An increase in cholesterol sulfate, phospholipids, sphingolipids and fatty acids associated with a decrease in glycerolipids suggest that the lipidome in mice skin is regulated by the Foxn1 gene.
authors.affiliation: UMR CNRS 8638, Faculté de Pharmacie, Université Paris Descartes, 4 Avenue de L'Observatoire, 75006 Paris, France.
authors.forename: Justine
authors.initials: J
authors.lastname: Lanzini
authors.affiliation: UMR CNRS 8638, Faculté de Pharmacie, Université Paris Descartes, 4 Avenue de L'Observatoire, 75006 Paris, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Dargère
authors.affiliation: UMR CNRS 8638, Faculté de Pharmacie, Université Paris Descartes, 4 Avenue de L'Observatoire, 75006 Paris, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Regazzetti
authors.affiliation: UMR CNRS 8638, Faculté de Pharmacie, Université Paris Descartes, 4 Avenue de L'Observatoire, 75006 Paris, France.
authors.forename: Abdellah
authors.initials: A
authors.lastname: Tebani
authors.affiliation: UMR CNRS 8638, Faculté de Pharmacie, Université Paris Descartes, 4 Avenue de L'Observatoire, 75006 Paris, France; AP-HP, Service de Toxicologie Biologique, Hôpital Lariboisière, 4 Rue Ambroise Paré, 75475 Paris Cedex 10, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Laprévote
authors.affiliation: UMR CNRS 8638, Faculté de Pharmacie, Université Paris Descartes, 4 Avenue de L'Observatoire, 75006 Paris, France. Electronic address: nicolas.auzeil@parisdescartes.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Auzeil
mesh_terms: D000818:Animals; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics*; D008055:Lipids / Q000032:analysis* / Q000235:genetics*; D013058:Mass Spectrometry; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009154:Mutation; D012867:Skin / Q000737:chemistry*
chemical_list: D051858:Forkhead Transcription Factors; D008055:Lipids; C090140:Whn protein
keywords: Foxn1; Lipidomics; Lipids; Mass spectrometry; Skin