title: Growth of Chitinophaga pinensis on Plant Cell Wall Glycans and Characterisation of a Glycoside Hydrolase Family 27 β-l-Arabinopyranosidase Implicated in Arabinogalactan Utilisation.
abstract: The genome of the soil bacterium Chitinophaga pinensis encodes a diverse array of carbohydrate active enzymes, including nearly 200 representatives from over 50 glycoside hydrolase (GH) families, the enzymology of which is essentially unexplored. In light of this genetic potential, we reveal that C. pinensis has a broader saprophytic capacity to thrive on plant cell wall polysaccharides than previously reported, and specifically that secretion of β-l-arabinopyranosidase activity is induced during growth on arabinogalactan. We subsequently correlated this activity with the product of the Cpin_5740 gene, which encodes the sole member of glycoside hydrolase family 27 (GH27) in C. pinensis, CpArap27. Historically, GH27 is most commonly associated with α-d-galactopyranosidase and α-d-N-acetylgalactosaminidase activity. A new phylogenetic analysis of GH27 highlighted the likely importance of several conserved secondary structural features in determining substrate specificity and provides a predictive framework for identifying enzymes with the less common β-l-arabinopyranosidase activity.
authors.affiliation: Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 106 91, Stockholm, Sweden; Wallenberg Wood Science Centre, Teknikringen 56-56, 100 44, Stockholm, Sweden.
authors.forename: Lauren S
authors.initials: LS
authors.lastname: McKee
authors.affiliation: Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 106 91, Stockholm, Sweden; Wallenberg Wood Science Centre, Teknikringen 56-56, 100 44, Stockholm, Sweden; Michael Smith Laboratories and Department of Chemistry, University of British Columbia, 2185 East Mall, Vancouver, V6T 1Z4, BC, Canada.
authors.forename: Harry
authors.initials: H
authors.lastname: Brumer
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000502:physiology*; D041963:Bacteroidetes / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D050260:Carbohydrate Metabolism; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D005685:Galactans / Q000378:metabolism*; D005798:Genes, Bacterial; D006026:Glycoside Hydrolases / Q000502:physiology*; D007700:Kinetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D059828:Plant Cells / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D005685:Galactans; D006026:Glycoside Hydrolases; C005653:arabinogalactan