title: FTIR microscopy reveals distinct biomolecular profile of crustacean digestive glands upon subtoxic exposure to ZnO nanoparticles.
abstract: Biomolecular profiling with Fourier-Transform InfraRed Microscopy was performed to distinguish the Zn(2+)-mediated effects on the crustacean (Porcellio scaber) digestive glands from the ones elicited by the ZnO nanoparticles (NPs). The exposure to ZnO NPs or ZnCl2 (1500 and 4000 µg Zn/g of dry food) activated different types of metabolic pathways: some were found in the case of both substances, some only in the case of ZnCl2, and some only upon exposure to ZnO NPs. Both the ZnO NPs and the ZnCl2 increased the protein (∼1312 cm(-1); 1720-1485 cm(-1)/3000-2830 cm(-1)) and RNA concentration (∼1115 cm(-1)). At the highest exposure concentration of ZnCl2, where the effects occurred also at the organismal level, some additional changes were found that were not detected upon the ZnO NP exposure. These included changed carbohydrate (most likely glycogen) concentrations (∼1043 cm(-1)) and the desaturation of cell membrane lipids (∼3014 cm(-1)). The activation of novel metabolic pathways, as evidenced by changed proteins' structure (at 1274 cm(-1)), was found only in the case of ZnO NPs. This proves that Zn(2+) are not the only inducers of the response to ZnO NPs. Low bioavailable fraction of Zn(2+) in the digestive glands exposed to ZnO NPs further supports the role of particles in the ZnO NP-generated effects. This study provides the evidence that ZnO NPs induce their own metabolic responses in the subtoxic range.
authors.affiliation: a Department of Biology, Biotechnical Faculty , University of Ljubljana , Ljubljana , Slovenia .
authors.forename: Tea
authors.initials: T
authors.lastname: Romih
authors.affiliation: a Department of Biology, Biotechnical Faculty , University of Ljubljana , Ljubljana , Slovenia .
authors.forename: Anita
authors.initials: A
authors.lastname: Jemec
authors.affiliation: a Department of Biology, Biotechnical Faculty , University of Ljubljana , Ljubljana , Slovenia .
authors.forename: Sara
authors.initials: S
authors.lastname: Novak
authors.affiliation: b Elettra-Sincrotrone Trieste S.C.p.A. , Basovizza , Trieste , Italy , and.
authors.forename: Lisa
authors.initials: L
authors.lastname: Vaccari
authors.affiliation: b Elettra-Sincrotrone Trieste S.C.p.A. , Basovizza , Trieste , Italy , and.
authors.forename: Paolo
authors.initials: P
authors.lastname: Ferraris
authors.affiliation: a Department of Biology, Biotechnical Faculty , University of Ljubljana , Ljubljana , Slovenia .
authors.forename: Martin
authors.initials: M
authors.lastname: Šimon
authors.affiliation: a Department of Biology, Biotechnical Faculty , University of Ljubljana , Ljubljana , Slovenia .
authors.forename: Monika
authors.initials: M
authors.lastname: Kos
authors.affiliation: c Department of Chemistry and Biochemistry, Faculty of Chemistry and Chemical Technology , University of Ljubljana , Ljubljana , Slovenia.
authors.forename: Robert
authors.initials: R
authors.lastname: Susič
authors.affiliation: c Department of Chemistry and Biochemistry, Faculty of Chemistry and Chemical Technology , University of Ljubljana , Ljubljana , Slovenia.
authors.forename: Ksenija
authors.initials: K
authors.lastname: Kogej
authors.affiliation: a Department of Biology, Biotechnical Faculty , University of Ljubljana , Ljubljana , Slovenia .
authors.forename: Jernej
authors.initials: J
authors.lastname: Zupanc
authors.affiliation: a Department of Biology, Biotechnical Faculty , University of Ljubljana , Ljubljana , Slovenia .
authors.forename: Damjana
authors.initials: D
authors.lastname: Drobne
mesh_terms: D000818:Animals; D002712:Chlorides / Q000009:adverse effects; D004064:Digestive System / Q000187:drug effects* / Q000378:metabolism*; D033321:Isopoda / Q000187:drug effects* / Q000378:metabolism; D008853:Microscopy; D053758:Nanoparticles / Q000008:administration & dosage / Q000009:adverse effects*; D015398:Signal Transduction / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D017967:Zinc Compounds / Q000009:adverse effects; D015034:Zinc Oxide / Q000008:administration & dosage* / Q000009:adverse effects*
chemical_list: D002712:Chlorides; D017967:Zinc Compounds; C016837:zinc chloride; D015034:Zinc Oxide
keywords: Changed protein structure; Porcellio scaber; ZnCl2; ZnO dissolution; square-wave voltammetry