title: Microarray Analysis of the Gene Expression Profile and Lipid Metabolism in Fat-1 Transgenic Cattle.
abstract: Long-chain n-3 polyunsaturated fatty acids (n-3 PUFAs) are beneficial for human health. However, humans and mammals are unable to synthesize n-3 PUFAs because they lack the n-3 desaturase gene fat-1 and must therefore obtain this type of fatty acid through their diet. Through the production of fat-1 transgenic animals, it is possible to obtain animal products that are rich in n-3 PUFAs, such as meat and milk. The aim of this study was to analyze the gene expression profile and the mechanism of lipid metabolism in fat-1 transgenic cattle and to accumulate important basic data that are required to obtain more efficient fat-1 transgenic cattle. Transcriptome profiling of fat-1 transgenic and wild-type cattle identified differentially expressed genes that are involved in 90 biological pathways, eight pathways of which were related to lipid metabolism processes 36 genes of which were related to lipid metabolism. This analysis also identified 11 significantly enriched genes that were involved in the peroxisome proliferator-activated receptor signaling pathway. These findings were verified by quantitative polymerase chain reaction. The information obtained in this study indicated that the introduction of an exogenous fat-1 gene into cattle affects the gene expression profile and the process of lipid metabolism in these animals. These results may provide important insights into how an exogenous fat-1 gene synthesizes n-3 PUFAs in transgenic cattle and other mammals.
authors.affiliation: The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot, China; College of Animal Science and Animal Medicine, Tianjin Agriculture University, Tianjin, China.
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Bai
authors.affiliation: College of Animal Science and Animal Medicine, Tianjin Agriculture University, Tianjin, China.
authors.forename: Xiangbin
authors.initials: X
authors.lastname: Ding
authors.affiliation: The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot, China.
authors.forename: Zhuying
authors.initials: Z
authors.lastname: Wei
authors.affiliation: College of Animal Science and Animal Medicine, Tianjin Agriculture University, Tianjin, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
authors.affiliation: The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot, China.
authors.forename: Guangpeng
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D002417:Cattle; D044943:Fatty Acid Desaturases / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D063990:Gene Ontology; D050356:Lipid Metabolism / Q000235:genetics*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D047492:Peroxisome Proliferator-Activated Receptors / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; D047492:Peroxisome Proliferator-Activated Receptors; C466741:fat-1 protein, C elegans; D044943:Fatty Acid Desaturases