title: Characterization of goat colostrum oligosaccharides by nano-liquid chromatography on chip quadrupole time-of-flight mass spectrometry and hydrophilic interaction liquid chromatography-quadrupole mass spectrometry.
abstract: A detailed qualitative and quantitative characterization of goat colostrum oligosaccharides (GCO) has been carried out for the first time. Defatted and deproteinized colostrum samples, previously treated by size exclusion chromatography (SEC) to remove lactose, were analyzed by nanoflow liquid chromatography-quadrupole-time of flight mass spectrometry (Nano-LC-Chip-Q-TOF MS). Up to 78 oligosaccharides containing hexose, hexosamine, fucose, N-acetylneuraminic acid or N-glycolylneuraminic acid monomeric units were identified in the samples, some of them detected for the first time in goat colostra. As a second step, a hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS) methodology was developed for the separation and quantitation of the main GCO, both acidic and neutral carbohydrates. Among other experimental chromatographic conditions, mobile phase additives and column temperature were evaluated in terms of retention time, resolution, peak width and symmetry of target carbohydrates. Narrow peaks (wh: 0.2-0.6min) and good symmetry (As: 0.8-1.4) were obtained for GCO using an acetonitrile:water gradient with 0.1% ammonium hydroxide at 40°C. These conditions were selected to quantify the main oligosaccharides in goat colostrum samples. Values ranging from 140 to 315mgL(-1) for neutral oligosaccharides and from 83 to 251mgL(-1) for acidic oligosaccharides were found. The combination of both techniques resulted to be useful to achieve a comprehensive characterization of GCO.
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Martín-Ortiz
authors.affiliation: Department of Food Science and Technology, University of California Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: J
authors.initials: J
authors.lastname: Salcedo
authors.affiliation: Department of Food Science and Technology, University of California Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: D
authors.initials: D
authors.lastname: Barile
authors.affiliation: Department of Food Science and Technology, University of California Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: A
authors.initials: A
authors.lastname: Bunyatratchata
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM), Universidad Autónoma de Madrid, C/Nicolás Cabrera 9, Campus de Cantoblanco, 28049 Madrid, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Moreno
authors.affiliation: Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Martin-García
authors.affiliation: Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Clemente
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain. Electronic address: mlsanz@iqog.csic.es.
authors.forename: M L
authors.initials: ML
authors.lastname: Sanz
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: A I
authors.initials: AI
authors.lastname: Ruiz-Matute
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D064753:Ammonium Hydroxide / Q000737:chemistry; D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D002853:Chromatography, Liquid; D003126:Colostrum / Q000737:chemistry*; D005260:Female; D006041:Goats; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000737:chemistry*; D011247:Pregnancy
chemical_list: D000097:Acetonitriles; D009844:Oligosaccharides; D064753:Ammonium Hydroxide; C032159:acetonitrile
keywords: Goat colostrum; Hydrophilic interaction liquid chromatography; Mass spectrometry; Nanoflow liquid chromatography–quadrupole-time of flight mass spectrometry; Oligosaccharides