title: Mixed hemimicelles solid-phase extraction based on sodium dodecyl sulfate (SDS)-coated nano-magnets for the spectrophotometric determination of Fingolomid in biological fluids.
abstract: In this study, mixed hemimicelles solid-phase extraction (SPE) based on sodium dodecyl sulfate (SDS)-coated nano-magnets Fe3O4 was investigated as a novel method for the separation and determination of Fingolimod (FLM) in water, urine and plasma samples prior to spectrophotometeric determination. Due to the high surface area of these new sorbents and the excellent adsorption capacity after surface modification by SDS, satisfactory extraction recoveries can be produced. The main factors affecting the adsolubilization of analysts, such as pH, surfactant and adsorbent amounts, ionic strength, extraction time and desorption conditions were studied and optimized. Under the selected conditions, FLM has been quantitatively extracted. The accuracy of the method was evaluated by recovery measurements on spiked samples, and good recoveries of 96%, 95% and 88% were observed for water, urine and plasma respectively. Proper linear behaviors over the investigated concentration ranges of 2-26, 2-17 and 2-13 mg/L with good coefficients of determination, 0.998, 0.997 and 0.995 were achieved for water, urine and plasma samples, respectively. To the best of our knowledge, this is the first time that a mixed hemimicelles SPE method based on magnetic separation and nanoparticles has been used as a simple and sensitive method for monitoring of FLM in water and biological samples.
authors.affiliation: Department of Chemistry, Payame Noor University (PNU), P. O. Box 19395-3697, Tehran, Iran.
authors.forename: Zhila
authors.initials: Z
authors.lastname: Azari
authors.affiliation: Department of Chemistry, Payame Noor University (PNU), P. O. Box 19395-3697, Tehran, Iran. Electronic address: pourbasheer@ut.ac.ir.
authors.forename: Eslam
authors.initials: E
authors.lastname: Pourbasheer
authors.affiliation: Department of Chemistry, Payame Noor University (PNU), P. O. Box 19395-3697, Tehran, Iran.
authors.forename: Abolghasem
authors.initials: A
authors.lastname: Beheshti
mesh_terms: D000327:Adsorption; D001826:Body Fluids / Q000737:chemistry*; D005260:Female; D000068876:Fingolimod Hydrochloride / Q000097:blood* / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D060328:Magnetic Phenomena; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009994:Osmolar Concentration; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D013053:Spectrophotometry; D013055:Spectrophotometry, Infrared; D013997:Time Factors
chemical_list: D008823:Micelles; D012967:Sodium Dodecyl Sulfate; D000068876:Fingolimod Hydrochloride
keywords: Fingolimod; Iron nanoparticles; Magnetic solid-phase extraction; Mixed-hemimicelles