title: Structural characterisation and anti-ageing activity of extracellular polysaccharide from a strain of Lachnum sp.
abstract: A homogeneous extracellular polysaccharide of Lachnum YM261(LEPS-1) with a molecular weight of 21670Da was characterised. According to HPGPC, IR, periodate oxidation and Smith degradation, GC-MS and (1)H NMR analysis, the results indicated that LEPS-1 was a glucan linked by the β-(1→3)-d-pyran glycosidic bond. The effect of LEPS-1 on anti-ageing in d-gal model mice was also studied. It was found that LEPS-1 significantly increased the activities of antioxidant enzymes (i.e. SOD superoxide dismutase, CAT catalase, GSH-PX glutathione peroxidase) and decreased malondialdehyde (MDA) content in liver, brain and serum of d-gal model mice. These results showed that LEPS-1 had a strong anti-ageing activity.
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address: yeming123@sina.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Ye
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Wu-Xi
authors.initials: WX
authors.lastname: Chen
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Qiu
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Ru-Yue
authors.initials: RY
authors.lastname: Yuan
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Ying-Wang
authors.initials: YW
authors.lastname: Ye
authors.affiliation: Department of Biological and Environmental Engineering, Hefei University, Hefei 230022, China.
authors.forename: Jing-Min
authors.initials: JM
authors.lastname: Cai
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides
keywords: Anti-ageing activity; Exopolysaccharide; Lachnum; Purification; β-(1→3)-d-Glucan