title: Effects of Intracoronary Administration of Autologous Adipose Tissue-Derived Stem Cells on Acute Myocardial Infarction in a Porcine Model.
abstract: OBJECTIVE
  Adipose-derived stem cells (ADSCs) are known to be potentially effective in regeneration of damaged tissue. We aimed to assess the effectiveness of intracoronary administration of ADSCs in reducing the infarction area and improving function after acute transmural myocardial infarction (MI) in a porcine model.
  
  
  METHODS
  ADSCs were obtained from each pig's abdominal subcutaneous fat tissue by simple liposuction. After 3 passages of 14-days culture, 2 million ADSCs were injected into the coronary artery 30 min after acute transmural MI. At baseline and 4 weeks after the ADSC injection, 99mTc methoxyisobutylisonitrile-single photon emission computed tomography (MIBISPECT) was performed to evaluate the left ventricular volume, left ventricular ejection fraction (LVEF; %), and perfusion defects as well as the myocardial salvage (%) and salvage index. At 4 weeks, each pig was sacrificed, and the heart was extracted and dissected. Gross and microscopic analyses with specific immunohistochemistry staining were then performed.
  
  
  RESULTS
  Analysis showed improvement in the perfusion defect, but not in the LVEF in the ADSC group (n=14), compared with the control group (n=14) (perfusion defect, -13.0±10.0 vs. -2.6±12.0, p=0.019; LVEF, -8.0±15.4 vs. -15.9±14.8, p=0.181). There was a tendency of reducing left ventricular volume in ADSC group. The ADSCs identified by stromal cell-derived factor-1 (SDF-1) staining were well co-localized by von Willebrand factor and Troponin T staining.
  
  
  CONCLUSIONS
  Intracoronary injection of cultured ADSCs improved myocardial perfusion in this porcine acute transmural MI model.
authors.affiliation: Division of Cardiology, Pusan National University Hospital, Busan, Korea.|Medical Research Institute, Pusan National University Hospital, Busan, Korea.
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Lee
authors.affiliation: Medical Research Institute, Pusan National University Hospital, Busan, Korea.|Division of Cardiology, Pusan National University Hospital, Busan, Korea. glaraone@hanmail.net.
authors.forename: Han Cheol
authors.initials: HC
authors.lastname: Lee
authors.affiliation: Division of Cardiology, Pusan National University Hospital, Busan, Korea.|Medical Research Institute, Pusan National University Hospital, Busan, Korea.
authors.forename: Jong Ha
authors.initials: JH
authors.lastname: Park
authors.affiliation: Division of Cardiology, Pusan National University Hospital, Busan, Korea.
authors.forename: Bo Won
authors.initials: BW
authors.lastname: Kim
authors.affiliation: Division of Cardiology, Pusan National University Hospital, Busan, Korea.
authors.forename: Jinhee
authors.initials: J
authors.lastname: Ahn
authors.affiliation: Division of Cardiology, Pusan National University Hospital, Busan, Korea.
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Division of Cardiology, Pusan National University Hospital, Busan, Korea.|Medical Research Institute, Pusan National University Hospital, Busan, Korea.
authors.forename: Jin Sup
authors.initials: JS
authors.lastname: Park
authors.affiliation: Division of Cardiology, Pusan National University Hospital, Busan, Korea.|Medical Research Institute, Pusan National University Hospital, Busan, Korea.
authors.forename: Jun-Hyok
authors.initials: JH
authors.lastname: Oh
authors.affiliation: Division of Cardiology, Pusan National University Hospital, Busan, Korea.|Medical Research Institute, Pusan National University Hospital, Busan, Korea.
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Division of Cardiology, Pusan National University Hospital, Busan, Korea.|Medical Research Institute, Pusan National University Hospital, Busan, Korea.
authors.forename: Kwang Soo
authors.initials: KS
authors.lastname: Cha
authors.affiliation: Division of Cardiology, Pusan National University Hospital, Busan, Korea.|Medical Research Institute, Pusan National University Hospital, Busan, Korea.
authors.forename: Taek Jong
authors.initials: TJ
authors.lastname: Hong
authors.affiliation: Division of Cardiology, Pusan National University Hospital, Busan, Korea.
authors.forename: Tae Sik
authors.initials: TS
authors.lastname: Park
authors.affiliation: Medical Research Institute, Pusan National University Hospital, Busan, Korea.|Division of Thoracic Surgery, Pusan National University Hospital, Busan, Korea.
authors.forename: Sang-Pil
authors.initials: SP
authors.lastname: Kim
authors.affiliation: Medical Research Institute, Pusan National University Hospital, Busan, Korea.|Division of Thoracic Surgery, Pusan National University Hospital, Busan, Korea.
authors.forename: Seunghwan
authors.initials: S
authors.lastname: Song
authors.affiliation: Division of Pathology, Pusan National University Hospital, Busan, Korea.
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Medical Research Institute, Pusan National University Hospital, Busan, Korea.|Division of Pathology, Pusan National University Hospital, Busan, Korea.
authors.forename: Mi Hwa
authors.initials: MH
authors.lastname: Park
authors.affiliation: Medical Research Institute, Pusan National University Hospital, Busan, Korea.|Division of Physiology, Pusan National University Hospital, Busan, Korea.
authors.forename: Jin Sup
authors.initials: JS
authors.lastname: Jung
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D054377:Chemokine CXCL12; D003331:Coronary Vessels; D005260:Female; D006321:Heart / Q000503:physiopathology; D006352:Heart Ventricles; D059630:Mesenchymal Stem Cells; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000503:physiopathology / Q000628:therapy*; D033581:Stem Cell Transplantation; D013552:Swine; D017256:Technetium Tc 99m Sestamibi / Q000494:pharmacology*; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*; D020107:Troponin T; D016277:Ventricular Function, Left
chemical_list: D054377:Chemokine CXCL12; D020107:Troponin T; D017256:Technetium Tc 99m Sestamibi
keywords: 99mTc MIBI; Myocardial infarction; angiogenesis; left ventricle remodeling; mesenchymal stem cells