title: Chlorpromazine transformation by exposure to ultraviolet laser beams in droplet and bulk.
abstract: Multiple drug resistance requires a flexible approach to find medicines able to overcome it. One method could be the exposure of existing medicines to ultraviolet laser beams to generate photoproducts that are efficient against bacteria and/or malignant tumors. This can be done in droplets or bulk volumes. In the present work are reported results about the interaction of 266nm and 355nm pulsed laser radiation with microdroplets and bulk containing solutions of 10mg/ml Chlorpromazine Hydrochloride (CPZ) in ultrapure water. The irradiation effects on CPZ solution at larger time intervals (more than 30min) are similar in terms of generated photoproducts if the two ultraviolet wavelengths are utilized. The understanding of the CPZ parent compound transformation may be better evidenced, as shown in this paper, if studies at shorter than 30minute exposure times are made coupled with properly chosen volumes to irradiate. We show that at exposure to a 355nm laser beam faster molecular modifications of CPZ in ultrapure water solution are produced than at irradiation with 266nm, for both microdroplet and bulk volume samples. These effects are evidenced by thin layer chromatography technique and laser induced fluorescence measurements.
authors.affiliation: National Institute for Lasers, Plasma and Radiation Physics, PO Box MG 36, str. Atomistilor 409, 077125 Magurele, Ilfov, Romania. Electronic address: ionut.andrei@inflpr.ro.
authors.forename: Ionut Relu
authors.initials: IR
authors.lastname: Andrei
authors.affiliation: National Institute for Lasers, Plasma and Radiation Physics, PO Box MG 36, str. Atomistilor 409, 077125 Magurele, Ilfov, Romania; Faculty of Physics, University of Bucharest, PO Box MG 11, str. Atomistilor 405, 077125 Magurele, Ilfov, Romania. Electronic address: tatiana.alexandru@inflpr.ro.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Tozar
authors.affiliation: National Institute for Lasers, Plasma and Radiation Physics, PO Box MG 36, str. Atomistilor 409, 077125 Magurele, Ilfov, Romania. Electronic address: andra.dinache@inflpr.ro.
authors.forename: Andra
authors.initials: A
authors.lastname: Dinache
authors.affiliation: National Institute for Lasers, Plasma and Radiation Physics, PO Box MG 36, str. Atomistilor 409, 077125 Magurele, Ilfov, Romania; Faculty of Physics, University of Bucharest, PO Box MG 11, str. Atomistilor 405, 077125 Magurele, Ilfov, Romania. Electronic address: mihai.boni@inflpr.ro.
authors.forename: Mihai
authors.initials: M
authors.lastname: Boni
authors.affiliation: National Institute for Lasers, Plasma and Radiation Physics, PO Box MG 36, str. Atomistilor 409, 077125 Magurele, Ilfov, Romania; Faculty of Physics, University of Bucharest, PO Box MG 11, str. Atomistilor 405, 077125 Magurele, Ilfov, Romania. Electronic address: viorel.nastasa@inflpr.ro.
authors.forename: Viorel
authors.initials: V
authors.lastname: Nastasa
authors.affiliation: National Institute for Lasers, Plasma and Radiation Physics, PO Box MG 36, str. Atomistilor 409, 077125 Magurele, Ilfov, Romania; Faculty of Physics, University of Bucharest, PO Box MG 11, str. Atomistilor 405, 077125 Magurele, Ilfov, Romania. Electronic address: mihai.pascu@inflpr.ro.
authors.forename: Mihail Lucian
authors.initials: ML
authors.lastname: Pascu
mesh_terms: D014150:Antipsychotic Agents / Q000737:chemistry* / Q000528:radiation effects; D002746:Chlorpromazine / Q000737:chemistry* / Q000528:radiation effects; D002855:Chromatography, Thin Layer; D005453:Fluorescence; D007834:Lasers; D014466:Ultraviolet Rays
chemical_list: D014150:Antipsychotic Agents; D002746:Chlorpromazine
keywords: 2-Hydroxy Promazine; 2-Hydroxy Promazine sulfoxide; CPZ; CPZ-SO; Chlorpromazine; Chlorpromazine Hydrochloride; Chlorpromazine sulfoxide; Laser irradiation; Microdroplet; PZ; Photoproducts; Promazine; Promazine sulfoxide; Thioridazine; Water