title: Recent applications of CE- and HPLC-MS in the analysis of human fluids.
abstract: The present review intends to cover the literature on the use of CE-/LC-MS for the analysis of human fluids, from 2010 until present. It has been planned to provide an overview of the most recent practical applications of these techniques to less extensively used human body fluids, including, bronchoalveolar lavage fluid, synovial fluid, nipple aspirate, tear fluid, breast fluid, amniotic fluid, and cerumen. Potential pitfalls related to fluid collection and sample preparation, with particular attention to sample clean-up procedures, and methods of analysis, from the research laboratory to a clinical setting will also be addressed. While being apparent that proteomics/metabolomics represent the most prominent approaches for global identification/quantification of putative biomarkers for a variety of human diseases, evidence is also provided of the suitability of these sophisticated techniques for the detection of heterogeneous components carried by these fluids.
authors.affiliation: Department of Biology and Biotechnologies "L. Spallanzani,", Biochemistry Unit, University of Pavia, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Iadarola
authors.affiliation: Department of Biology and Biotechnologies "L. Spallanzani,", Biochemistry Unit, University of Pavia, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Fumagalli
authors.affiliation: Department of Molecular Medicine, Biochemistry Unit, University of Pavia, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Bardoni
authors.affiliation: Department of Molecular Medicine, Biochemistry Unit, University of Pavia, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Salvini
authors.affiliation: Department of Molecular Medicine, Biochemistry Unit, University of Pavia, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Viglio
mesh_terms: D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D006801:Humans; D013058:Mass Spectrometry; D055432:Metabolomics; D040901:Proteomics
keywords: CE-MS; Human body fluids; LC-MS; Metabolomics; Proteomics