title: Curcumin inhibits development and cell adhesion in Dictyostelium discoideum: Implications for YakA signaling and GST enzyme function.
abstract: The molecular basis for nutraceutical properties of the polyphenol curcumin (Curcuma longa, Turmeric) is complex, affecting multiple factors that regulate cell signaling and homeostasis. Here, we report the effect of curcumin on cellular and developmental mechanisms in the eukaryotic model, Dictyostelium discoideum. Dictyostelium proliferation was inhibited in the presence of curcumin, which also suppressed the prestarvation marker, discoidin I, members of the yakA-mediated developmental signaling pathway, and expression of the extracellular matrix/cell adhesion proteins (DdCAD and csA). This resulted in delayed chemotaxis, adhesion, and development of the organism. In contrast to the inhibitory effects on developmental genes, curcumin induced gstA gene expression, overall GST activity, and generated production of reactive oxygen species. These studies expand our knowledge of developmental and biochemical signaling influenced by curcumin, and lends greater consideration of GST enzyme function in eukaryotic cell signaling, development, and differentiation.
authors.affiliation: Department of Biochemistry and Molecular Biology, Howard University College of Medicine, Washington, DC, USA.
authors.forename: Mamatha
authors.initials: M
authors.lastname: Garige
authors.affiliation: Department of Biochemistry and Molecular Biology, Howard University College of Medicine, Washington, DC, USA. Electronic address: ewalters@howard.edu.
authors.forename: Eric
authors.initials: E
authors.lastname: Walters
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D004023:Dictyostelium / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D000070821:Discoidins; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D018507:Gene Expression Regulation, Developmental; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D037102:Lectins / Q000235:genetics / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D015815:Cell Adhesion Molecules; D000070821:Discoidins; D004791:Enzyme Inhibitors; D037102:Lectins; D015800:Protozoan Proteins; D017382:Reactive Oxygen Species; C052291:cell cohesion molecule, Dictyostelium; D005982:Glutathione Transferase; D011494:Protein Kinases; C113280:YakA protein, Dictyostelium discoideum; D003474:Curcumin
keywords: Development; Dictyostelium; GST enzymes; Polyphenol; YakA signaling