title: Induction Function of miR-126 in Survival and Proliferation in Neural Stem Cells.
abstract: BACKGROUND
  The aim of this study was to investigate the potential function of miR-126 in neural stem cells (NSCs).
  
  
  METHODS
  Expression level of miR-126 was detected by quantitative real-time PCR (qRT-PCR). MiR-126 overexpression was established by transfecting miR-126 mimics into human NSC lines (HB1.F3 and HB1.A4 cells). Its effects on cell proliferation were studied using cell-counting kit-8 (CCK8) assay, colony formation assays. Flow cytometry was performed to evaluate the effect of miR-126 on cell survival.
  
  
  RESULTS
  CCK8 assay and colony formation assay showed that overexpression of miR-126 promoted cell proliferation and increased colony numbers in HB1.F3 and HB1.A4 cells. The flow cytometry confirmed the results that miR-126 inhibited cell apoptosis.
  
  
  CONCLUSIONS
  MiR-126 promoted the proliferation and survival of NSCs.
authors.affiliation: Department of Emergency, Pingyang People's Hospital, Wenzhou, Zhejiang, China (mainland).
authors.forename: Qijun
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Emergency, Pingyang People's Hospital, Wenzhou, Zhejiang, China (mainland).
authors.forename: Sheng
authors.initials: S
authors.lastname: Zeng
authors.affiliation: Department of Emergency, Pingyang People's Hospital, Wenzhou, Zhejiang, China (mainland).
authors.forename: Chengyuan
authors.initials: C
authors.lastname: Quan
authors.affiliation: Department of Emergency, Pingyang People's Hospital, Wenzhou, Zhejiang, China (mainland).
authors.forename: Xiaopo
authors.initials: X
authors.lastname: Lin
mesh_terms: D017209:Apoptosis; D001921:Brain / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D005434:Flow Cytometry; D006801:Humans; D007249:Inflammation; D016086:Lentivirus / Q000235:genetics; D035683:MicroRNAs / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: C527111:MIRN126 microRNA, human; D035683:MicroRNAs