title: Sensitive Detection of Deliquescent Bacterial Capsules through Nanomechanical Analysis.
abstract: Encapsulated bacteria usually exhibit strong resistance to a wide range of sterilization methods, and are often virulent. Early detection of encapsulation can be crucial in microbial pathology. This work demonstrates a fast and sensitive method for the detection of encapsulated bacterial cells. Nanoindentation force measurements were used to confirm the presence of deliquescent bacterial capsules surrounding bacterial cells. Force/distance approach curves contained characteristic linear-nonlinear-linear domains, indicating cocompression of the capsular layer and cell, indentation of the capsule, and compression of the cell alone. This is a sensitive method for the detection and verification of the encapsulation status of bacterial cells. Given that this method was successful in detecting the nanomechanical properties of two different layers of cell material, i.e. distinguishing between the capsule and the remainder of the cell, further development may potentially lead to the ability to analyze even thinner cellular layers, e.g. lipid bilayers.
authors.affiliation: Faculty of Science, Engineering and Technology, Swinburne University of Technology , P.O. Box 218, Hawthorn 3122, Victoria, Australia.
authors.forename: Song Ha
authors.initials: SH
authors.lastname: Nguyen
authors.affiliation: Faculty of Science, Engineering and Technology, Swinburne University of Technology , P.O. Box 218, Hawthorn 3122, Victoria, Australia.
authors.forename: Hayden K
authors.initials: HK
authors.lastname: Webb
mesh_terms: D016667:Bacterial Capsules / Q000737:chemistry*; D036103:Nanotechnology; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000166:cytology