title: Increased Hydrolysis of Oximino-β-Lactams by CMY-107, a Tyr199Cys Mutant Form of CMY-2 Produced by Escherichia coli.
abstract: The cephalosporinase CMY-107, a Tyr199Cys mutant form of CMY-2 encoded by an IncI self-transferable plasmid carried by an Escherichia coli clinical strain, was characterized. The enzyme hydrolyzed oximino-cephalosporins and aztreonam more efficiently than CMY-2 did.
authors.affiliation: Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece skotsakis@pasteur.gr miriagou@pasteur.gr.
authors.forename: S D
authors.initials: SD
authors.lastname: Kotsakis
authors.affiliation: Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece skotsakis@pasteur.gr miriagou@pasteur.gr.
authors.forename: V
authors.initials: V
authors.lastname: Miriagou
authors.affiliation: Department of Biopathology, A. & P. Kyriakou Children's Hospital, Athens, Greece.
authors.forename: E E
authors.initials: EE
authors.lastname: Vetouli
authors.affiliation: Department of Biopathology, A. & P. Kyriakou Children's Hospital, Athens, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Bozavoutoglou
authors.affiliation: Department of Biopathology, A. & P. Kyriakou Children's Hospital, Athens, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Lebessi
authors.affiliation: Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Tzelepi
authors.affiliation: Department of Microbiology, Medical School, University of Athens, Athens, Greece.
authors.forename: L S
authors.initials: LS
authors.lastname: Tzouvelekis
mesh_terms: D019943:Amino Acid Substitution; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002511:Cephalosporins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D056426:Genetic Loci; D006801:Humans; D006868:Hydrolysis; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D058885:Multilocus Sequence Typing; D009154:Mutation; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013379:Substrate Specificity; D014443:Tyrosine / Q000737:chemistry / Q000378:metabolism; D001618:beta-Lactamases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D029968:Escherichia coli Proteins; D007527:Isoenzymes; D014443:Tyrosine; D001618:beta-Lactamases; C000605810:beta-lactamase CMY-2, E coli; D003545:Cysteine