title: Predomination of IL-17-producing tryptase-positive/chymase-positive mast cells in azoospermic chronic testicular inflammation.
abstract: Chronic testicular inflammation and infection have been regarded as important factors in the pathogenesis of azoospermia. As key effector cells in innate and adaptive immune system, mast cells (MCs) were observed in inflammation and autoimmune disease. Furthermore, increased expression of tryptase-positive MCs has been reported in testicular disorders associated with male infertility/subfertility. However, little is known about the potential relationship between MCs and chronic testicular inflammation in azoospermic patients. Moreover, the preferential expression of MCs' subtypes in testis of these patients is still far from being understood. Thus, this study aimed to investigate characteristics of testicular MCs as well as their subtypes in azoospermic men with chronic testicular inflammation (AZI, n = 5) by immunohistochemical techniques. Our results showed significant increase of MCs in AZI, and more importantly, considerable numbers of tryptase-positive/chymase-positive MCs could also be demonstrated in AZI, when compared to control groups representing azoospermia without chronic testicular inflammation (AZW, n = 5) and normal spermatogenesis (NT, n = 5) respectively. Most interestingly, immunofluorescence staining revealed autoimmune-associated interleukin (IL)-17-producing MCs in AZI, whereas co-expression of MC markers with tumour necrosis factor (TNF)-α, IL-10 and IL-1β could not be detected. In conclusion, AZI is associated with significant increase of tryptase-positive/chymase-positive MCs expressing IL-17, and these MCs might contribute to the pathogenesis of AZI.
authors.affiliation: Department of Dermatology/Andrology Unit, Rheinische Friedrich Wilhelm University, Bonn, Germany.
authors.forename: S-J
authors.initials: SJ
authors.lastname: Chen
authors.affiliation: Department of Dermatology/Andrology Unit, Rheinische Friedrich Wilhelm University, Bonn, Germany.
authors.forename: Y-G
authors.initials: YG
authors.lastname: Duan
authors.affiliation: Department of Dermatology/Andrology Unit, Rheinische Friedrich Wilhelm University, Bonn, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Haidl
authors.affiliation: Department of Dermatology/Andrology Unit, Rheinische Friedrich Wilhelm University, Bonn, Germany.
authors.forename: J-P
authors.initials: JP
authors.lastname: Allam
mesh_terms: D053713:Azoospermia / Q000378:metabolism* / Q000473:pathology; D053818:Chymases / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D016753:Interleukin-10 / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D013737:Testis / Q000378:metabolism* / Q000473:pathology; D053802:Tryptases / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D020381:Interleukin-17; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D053818:Chymases; D053802:Tryptases
keywords: Azoospermia; IL-17; MC tryptase/chymase; chronic testicular inflammation; immunohistochemistry