title: Circulating CD4(+)CXCR5(+) T Cells Exacerbate B Cell Antibody Production in Crohn's Disease Through IL-21 Secretion.
abstract: Interleukin-21 (IL-21) upregulation was observed in Crohn's disease (CD) patients and was shown to contribute to ongoing mucosal inflammation in CD patients through stabilizing Th1 cell differentiation and IFN-γ production. Given the role of IL-21 in mediating adaptive B cell antibody responses in healthy individuals, we examined the effect of IL-21 upregulation in B cell responses in patients with active CD, including ileum, ileocolonic and colon subtypes, defined by the primary site of CD involvement. We first observed an upregulation of blood plasma IL-21 concentration and IL-21 production from CD4(+) T cells in CD patients compared to healthy individuals. The IL-21-expressing T cells were more concentrated in the CD4(+)CXCR5(+) compartment, both in unstimulated medium and after stimulation with SEB. ICOS and PD-1 expressions were also concentrated in the CD4(+)CXCR5(+) subset in CD patients. Since peripheral blood CD4(+)CXCR5(+) T cell-mediated antibody secretion is IL-21-dependent, we examined the plasma antibody concentration in CD patients and healthy controls. We found that CD patients had significantly higher plasma Ig level than healthy patients, with no significant differences between different CD subtypes. Higher plasma IL-21 concentration and increased IL-21 production from CD4(+) T cells were directly correlated with higher plasma antibody levels. Moreover, we found that IL-21 and CD4(+)CXCR5(+) T cells can directly enhance B cell antibody response in CD patients. Depletion of secreted IL-21 by sIL-21R addition compromised the CD4(+)CXCR5(+) T cell-mediated increase in antibody production. Together, our results demonstrated a novel role of IL-21 in mediating B cell inflammation in CD development.
authors.affiliation: a Department of General Surgery , Jinling Hospital, Medical School of Nanjing University , Nanjing , China .
authors.forename: Zhenlong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: a Department of General Surgery , Jinling Hospital, Medical School of Nanjing University , Nanjing , China .
authors.forename: Lan
authors.initials: L
authors.lastname: Ding
authors.affiliation: a Department of General Surgery , Jinling Hospital, Medical School of Nanjing University , Nanjing , China .
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Wang
authors.affiliation: a Department of General Surgery , Jinling Hospital, Medical School of Nanjing University , Nanjing , China .
authors.forename: Jinjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: a Department of General Surgery , Jinling Hospital, Medical School of Nanjing University , Nanjing , China .
authors.forename: Nan
authors.initials: N
authors.lastname: Zhu
authors.affiliation: a Department of General Surgery , Jinling Hospital, Medical School of Nanjing University , Nanjing , China .
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Diao
authors.affiliation: b Medical Center, University of Maryland , Baltimore , MD , USA .
authors.forename: Todd A
authors.initials: TA
authors.lastname: Demmy
authors.affiliation: b Medical Center, University of Maryland , Baltimore , MD , USA .
authors.forename: Jack M
authors.initials: JM
authors.lastname: Haitsma
authors.affiliation: b Medical Center, University of Maryland , Baltimore , MD , USA .
authors.forename: Ewa E
authors.initials: EE
authors.lastname: Lech-Maranda
authors.affiliation: c Tufts Medical Center, Tufts Boston University , Boston , MA , USA , and.
authors.forename: Naveen N
authors.initials: NN
authors.lastname: Bangia
authors.affiliation: c Tufts Medical Center, Tufts Boston University , Boston , MA , USA , and.
authors.forename: Myron A
authors.initials: MA
authors.lastname: Czuczman
authors.affiliation: a Department of General Surgery , Jinling Hospital, Medical School of Nanjing University , Nanjing , China .
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Qian
authors.affiliation: d University Hospital, University of British Columbia , Vancouver , BC , Canada.
authors.forename: Kalman L
authors.initials: KL
authors.lastname: Kovacs
mesh_terms: D000328:Adult; D000917:Antibody Formation / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D016022:Case-Control Studies; D003424:Crohn Disease / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007378:Interleukins / Q000097:blood / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D054380:Receptors, CXCR5 / Q000378:metabolism; D012720:Severity of Illness Index; D055815:Young Adult; D000098546:Interleukin-21
chemical_list: D007378:Interleukins; D054380:Receptors, CXCR5; D000098546:Interleukin-21
keywords: B cell; Crohn's disease; circulating; follicular helper T cell