title: Six-week anaerobic training improves proteolytic profile of diabetic rats.
abstract: OBJECTIVE
  To evaluate the effect of six-week anaerobic training on the mRNA expression of genes related to proteolysis Ubb (Ubiquitin), E2-14kDa, Trim63 (MuRF1 protein) and Nfkb1 in the skeletal muscle of diabetic rats.
  
  
  METHODS
  Four groups were established: DE (DiabetesExercised), DS (Diabetes Sedentary), CE (Control Exercised) and CS (Control Sedentary). The training consisted of 3 sets of 12 jumps in the liquid mean with load equivalent to 50% of BW for 6 weeks. Euthanasia occurred under ip anesthesia, and blood, adipose tissue and skeletal muscles were collected. Gene expression was quantified by RT-PCR in the gastrocnemius muscle. ANOVA one-way was used for comparison among groups, with post-hoc (Tukey) when necessary, considering p < 0.05.
  
  
  RESULTS
  We observed reduction in the body weight and adipose tissue in the diabetic groups. The muscle mass was reduced in DS, which could be reversed by training (DE). Although DS and DE have presented similar body weight, the training protocol in DE promoted reduction in the adipose tissue, and increase of muscle mass. Anaerobic training was efficient to reduce glycaemia only in the diabetic animals until 6 hours after the end of training. The Trim63 gene expression was increased in DS; decreased Ubb gene level was observed in trained rats (CE and DE) compared to sedentary (CS and DS), and DE presented the lowest level of E2-14kDa gene expression.
  
  
  CONCLUSIONS
  Six-week anaerobic training promoted muscle mass gain, improved glycemic control, and exerted inhibitory effect on the proteolysis of gastrocnemius muscle of diabetic rats.
authors.affiliation: Departamento de Fisioterapia, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, Presidente Prudente, SP, Brasil.
authors.forename: Rogério de
authors.initials: Rd
authors.lastname: Oliveira-Batista
authors.affiliation: Departamento de Medicina Veterinária, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil.
authors.forename: Angelita
authors.initials: A
authors.lastname: Silva
authors.affiliation: Departamento de Medicina Veterinária, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil.
authors.forename: Kaique Marques Rodrigues dos
authors.initials: KM
authors.lastname: Passos
authors.affiliation: Departamento de Medicina Veterinária, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil.
authors.forename: Rosa Maria Barilli
authors.initials: RM
authors.lastname: Nogueira
authors.affiliation: Departamento de Fisioterapia, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, Presidente Prudente, SP, Brasil.
authors.forename: Patricia Monteiro
authors.initials: PM
authors.lastname: Seraphim
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology; D000693:Anaerobiosis; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000502:physiology; D003920:Diabetes Mellitus / Q000378:metabolism*; D015870:Gene Expression; D008297:Male; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D059748:Proteolysis; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D017208:Rats, Wistar; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D012333:RNA, Messenger; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex