title: Effects of exercise on brain and peripheral inflammatory biomarkers induced by total sleep deprivation in rats.
abstract: BACKGROUND
  Physical exercise induces neuroprotection through anti-inflammatory effects and total sleep deprivation is reported an inflammatory process. We examined whether 7 weeks of exercise training attenuates markers of inflammation during total sleep deprivation (24-h wakefulness) in the rat brain and periphery.
  
  
  METHODS
  Four groups of 10 rats were investigated: Sedentary control, Sedentary sleep-deprived, Exercised control, and Exercised sleep-deprived. Sleep deprivation and exercise training were induced using slowly rotating wheels and a motorized treadmill. We examined mRNA expression of pro-inflammatory (IL-1β, TNF-α, and IL-6) cytokine-related genes using real-time PCR, and protein levels in the hippocampus and frontal cortex, as well as circulating concentrations.
  
  
  RESULTS
  Compared to Sedentary control rats, hippocampal and cortical IL-1β mRNA expressions in Sedentary sleep-deprived rats were up-regulated (p < 0.05 and p < 0.01 respectively). At the protein level, hippocampal IL-1β and TNF-α and cortical IL-6 contents were higher in Sedentary sleep-deprived rats (p < 0.001, p < 0.05, p < 0.05, respectively). Peripherally, TNF-α, IL-6 and norepinephrine concentrations were higher in Sedentary sleep-deprived rats compared to Sedentary control (p < 0.01, p < 0.001, p < 0.01, respectively). Exercise training reduced the sleep deprivation-induced hippocampal IL-1β increases (mRNA expression and protein content) (p < 0.05 and p < 0.001), and TNF-α content (p < 0.001). At the periphery, exercise reduced sleep deprivation-induced increase of IL-6 concentration (p < 0.05) without effect on TNF-α and norepinephrine.
  
  
  CONCLUSIONS
  We demonstrate that a 7-week exercise training program before acute total sleep deprivation prevents pro-inflammatory responses in the rat hippocampus, particularly the IL-1β cytokine at the gene expression level and protein content.
authors.affiliation: Département Neurosciences et contraintes opérationnelles, Institut de recherche biomédicale des armées, Brétigny-sur-Orge, France ; Université Paris Descartes, Sorbonne Paris Cité, EA7330 VIFASOM, Paris, France ; Armed Forces Biomedical Research Institute (IRBA), B.P.73, 91223 Brétigny-sur-Orge, Cedex France.
authors.forename: M
authors.initials: M
authors.lastname: Chennaoui
authors.affiliation: Département Neurosciences et contraintes opérationnelles, Institut de recherche biomédicale des armées, Brétigny-sur-Orge, France ; Université Paris Descartes, Sorbonne Paris Cité, EA7330 VIFASOM, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Gomez-Merino
authors.affiliation: Département Neurosciences et contraintes opérationnelles, Institut de recherche biomédicale des armées, Brétigny-sur-Orge, France ; Université Paris Descartes, Sorbonne Paris Cité, EA7330 VIFASOM, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Drogou
authors.affiliation: Centre National pour la Recherche Scientifique, UMR 8249, 10 rue Vauquelin, 75005 Paris, France ; Brain Plasticity Unit, ESPCI-ParisTech, 10 rue Vauquelin, 75005 Paris, France.
authors.forename: H
authors.initials: H
authors.lastname: Geoffroy
authors.affiliation: Département Neurosciences et contraintes opérationnelles, Institut de recherche biomédicale des armées, Brétigny-sur-Orge, France ; Université Paris Descartes, Sorbonne Paris Cité, EA7330 VIFASOM, Paris, France.
authors.forename: G
authors.initials: G
authors.lastname: Dispersyn
authors.affiliation: Département Neurosciences et contraintes opérationnelles, Institut de recherche biomédicale des armées, Brétigny-sur-Orge, France.
authors.forename: C
authors.initials: C
authors.lastname: Langrume
authors.affiliation: Département Neurosciences et contraintes opérationnelles, Institut de recherche biomédicale des armées, Brétigny-sur-Orge, France.
authors.forename: S
authors.initials: S
authors.lastname: Ciret
authors.affiliation: Centre National pour la Recherche Scientifique, UMR 8249, 10 rue Vauquelin, 75005 Paris, France ; Brain Plasticity Unit, ESPCI-ParisTech, 10 rue Vauquelin, 75005 Paris, France.
authors.forename: T
authors.initials: T
authors.lastname: Gallopin
authors.affiliation: Département Neurosciences et contraintes opérationnelles, Institut de recherche biomédicale des armées, Brétigny-sur-Orge, France ; Université Paris Descartes, Sorbonne Paris Cité, EA7330 VIFASOM, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Sauvet
keywords: Brain; Cytokines; Exercise; Hormones; Periphery; Sleep deprivation