title: How the SAC gets the axe: Integrating kinetochore microtubule attachments with spindle assembly checkpoint signaling.
abstract: Mitosis entails the bona fide segregation of duplicated chromosomes. This process is accomplished by the attachment of kinetochores on chromosomes to microtubules (MTs) of the mitotic spindle. Once the appropriate attachment is achieved, the spindle assembly checkpoint (SAC) that delays the premature onset of anaphase needs to be silenced for the cell to proceed to anaphase and cytokinesis. Therefore, while it is imperative to preserve the SAC when kinetochores are unattached, it is of paramount importance that SAC components are removed post kinetochore microtubule (kMT) attachment. Precise knowledge of how kMT attachments trigger the removal of SAC components from kinetochores or how the checkpoint proteins feedback in to the attachment machinery remains elusive. This review aims to describe the recent advances that provide an insight into the interplay of molecular events that coordinate and regulate the SAC activity in response to kMT attachment during cell division.
authors.affiliation: a Department of Cell and Molecular Biology ; Feinberg School of Medicine; Northwestern University ; Chicago , IL USA.
authors.forename: Shivangi
authors.initials: S
authors.lastname: Agarwal
authors.affiliation: a Department of Cell and Molecular Biology ; Feinberg School of Medicine; Northwestern University ; Chicago , IL USA.
authors.forename: Dileep
authors.initials: D
authors.lastname: Varma
mesh_terms: D059447:Cell Cycle Checkpoints / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D003598:Cytoskeletal Proteins; D005786:Gene Expression Regulation; D006801:Humans; D018386:Kinetochores / Q000378:metabolism* / Q000648:ultrastructure; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D008938:Mitosis; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D008941:Spindle Apparatus / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; C472643:Knl1 protein, human; D008869:Microtubule-Associated Proteins; C120529:NDC80 protein, human; D009687:Nuclear Proteins
keywords: KMN; Knl1; Ndc80; checkpoint; kinetochore; microtubule; mitosis; spindle