title: Quantification of Coumarins in Aqueous Extract of Pterocaulon balansae (Asteraceae) and Characterization of a New Compound.
abstract: Plants from the genus Pterocaulon are popularly used as antifungal and wound-healing agents. Such activities have been related to coumarins, which are abundant in those plants. Coumarins are soluble in organic solvents, such as hexane and dichloromethane, and some of them are also soluble in hot water. Considering that infusion and decoctions of these plants are used in traditional medicine, the aim of this study was to identify and quantify the coumarins in the aqueous extract of Pterocaulon balansae. The aqueous extract was obtained by dynamic maceration and the compounds were characterized by UPLC-UV-MS analysis. A new coumarin and 5-methoxy-6,7-methylenedioxycoumarin, used for validation of the analytical HPLC method were obtained by partition of the aqueous extract with n-hexane. The HPLC method validated was linear, specific, and precise. Seven coumarins were characterized in the aqueous extract in a range of 0.584-54 mg/g of dry plant material. The main compound, 5,6-dimethoxy-7-(3'-methyl-2',3'-dihydroxybutyloxy)coumarin, is described for the first time in P. balansae together with a new compound, 5,6-dimethoxy-7-(2',3'-epoxy-3'-methylbutyloxy)coumarin.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, Porto Alegre, RS 90610-000, Brazil. farmbrunamedeiros@gmail.com.
authors.forename: Bruna
authors.initials: B
authors.lastname: Medeiros-Neves
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, Porto Alegre, RS 90610-000, Brazil. barrosfarm@yahoo.com.br.
authors.forename: Francisco Maikon Corrêa
authors.initials: FM
authors.lastname: de Barros
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, Porto Alegre, RS 90610-000, Brazil. gilsane@farmacia.ufrgs.br.
authors.forename: Gilsane Lino
authors.initials: GL
authors.lastname: von Poser
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, Porto Alegre, RS 90610-000, Brazil. helder.teixeira@ufrgs.br.
authors.forename: Helder Ferreira
authors.initials: HF
authors.lastname: Teixeira
mesh_terms: D000935:Antifungal Agents / Q000032:analysis / Q000737:chemistry; D019659:Asteraceae / Q000737:chemistry*; D052117:Benzodioxoles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000032:analysis* / Q000737:chemistry; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: C580931:5-methoxy-6,7-methylenedioxycoumarin; D000935:Antifungal Agents; D052117:Benzodioxoles; D003374:Coumarins; D010936:Plant Extracts
keywords: 5,6-dimethoxy-7-(2′,3′-epoxy-3′-methylbutyloxy)coumarin; 5-methoxy-6,7-methylenedioxycoumarin; HPLC; Pterocaulon balansae; UPLC-UV-MS; coumarin