title: Immunocytochemical and Immunohistochemical Staining with Peptide Antibodies.
abstract: Peptide antibodies are particularly useful for immunocytochemistry (ICC) and immunohistochemistry (IHC), where antigens may denature due to fixation of tissues and cells. Peptide antibodies can be made to any defined sequence, including unknown putative proteins and posttranslationally modified sequences. Moreover, the availability of large amounts of the antigen (peptide) allows inhibition/adsorption controls, which are important in ICC/IHC, due to the many possibilities for false-positive reactions caused by immunoglobulin Fc receptors, nonspecific reactions, and cross-reactivity of primary and secondary antibodies with other antigens and endogenous immunoglobulins, respectively. Here, simple protocols for ICC and IHC are described together with recommendations for appropriate controls.
authors.affiliation: Department of Autoimmunology and Biomarkers, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark.
authors.forename: Tina
authors.initials: T
authors.lastname: Friis
authors.affiliation: Department of Autoimmunology and Biomarkers, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark.
authors.forename: Klaus Boberg
authors.initials: KB
authors.lastname: Pedersen
authors.affiliation: Department of Congenital Diseases, Statens Serum Institut, Copenhagen, Denmark.
authors.forename: David
authors.initials: D
authors.lastname: Hougaard
authors.affiliation: Department of Autoimmunology and Biomarkers, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark. gh@ssi.dk.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Houen
mesh_terms: D000906:Antibodies / Q000276:immunology* / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000941:Antigens / Q000276:immunology; D002460:Cell Line; D000939:Epitopes / Q000276:immunology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D010455:Peptides / Q000276:immunology* / Q000378:metabolism
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens; D000939:Epitopes; D010455:Peptides
keywords: Adsorption control; Immunocytochemistry; Immunohistochemistry; Peptide antibodies; Specific staining