title: Derivation and Chondrogenic Commitment of Human Embryonic Stem Cell-Derived Mesenchymal Progenitors.
abstract: The induction of human embryonic stem cells to a mesenchymal-like progenitor population constitutes a developmentally relevant approach for efficient directed differentiation of human embryonic stem (hES) cells to the chondrogenic lineage. The initial enrichment of a hemangioblast intermediate has been shown to yield a replenishable population of highly purified progenitor cells that exhibit the typical mesenchymal stem cell (MSC) surface markers as well as the capacity for multilineage differentiation to bone, fat, and cartilage. Herein, we provide detailed methodologies for the derivation and characterization of potent mesenchymal-like progenitors from hES cells and describe in vitro assays for bone morphogenetic protein (BMP)-2-mediated differentiation to the chondrogenic lineage.
authors.affiliation: Department of Orthopaedic Surgery, UConn Health, 263 Farmington Avenue, Farmington, CT, 06030-4037, USA. drissi@uchc.edu.|Department of Genetics and Genome Sciences, UConn Health, 400 Farmington Avenue, Farmington, CT, 06030-6403, USA. drissi@uchc.edu.|Stem Cell Institute, UConn Health, 400 Farmington Avenue, Farmington, CT, 06030-6403, USA. drissi@uchc.edu.
authors.forename: Hicham
authors.initials: H
authors.lastname: Drissi
authors.affiliation: Department of Orthopaedic Surgery, UConn Health, 263 Farmington Avenue, Farmington, CT, 06030-4037, USA.
authors.forename: Jason D
authors.initials: JD
authors.lastname: Gibson
authors.affiliation: Department of Orthopaedic Surgery, UConn Health, 263 Farmington Avenue, Farmington, CT, 06030-4037, USA.|Stem Cell Institute, UConn Health, 400 Farmington Avenue, Farmington, CT, 06030-6403, USA.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Guzzo
authors.affiliation: Department of Genetics and Genome Sciences, UConn Health, 400 Farmington Avenue, Farmington, CT, 06030-6403, USA.|Stem Cell Institute, UConn Health, 400 Farmington Avenue, Farmington, CT, 06030-6403, USA.
authors.forename: Ren-He
authors.initials: RH
authors.lastname: Xu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D020219:Chondrogenesis / Q000187:drug effects / Q000235:genetics; D018920:Coculture Techniques; D053595:Embryonic Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D061252:Feeder Cells; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D010641:Phenotype; D044968:Regenerative Medicine / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: C525718:BMP2 protein, human; D015415:Biomarkers; D055396:Bone Morphogenetic Protein 2
keywords: BMP-2; Chondrogenic differentiation; Hemangioblast; Human embryonic stem cells; Mesenchymal stem cells; Pellet culture