title: A MALDI-MSI Approach to the Characterization of Radiation-Induced Lung Injury and Medical Countermeasure Development.
abstract: Radiation-induced lung injury is highly complex and characterized by multiple pathologies, which occur over time and sporadically throughout the lung. This complexity makes biomarker investigations and medical countermeasure screenings challenging. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has the ability to resolve differences spatially in molecular profiles within the lung following radiation exposure and can aid in biomarker identification and pharmaceutical efficacy investigations. MALDI-MSI was applied to the investigation of a whole-thorax lung irradiation model in non-human primates (NHP) for lipidomic analysis and medical countermeasure distribution.
authors.affiliation: *University of Maryland, School of Pharmacy, Department of Pharmaceutical Sciences, Baltimore, MD 21201; †University of Maryland, School of Medicine, Department of Radiation Oncology, Baltimore, MD 21201.
authors.forename: Claire L
authors.initials: CL
authors.lastname: Carter
authors.forename: Jace W
authors.initials: JW
authors.lastname: Jones
authors.forename: Kory
authors.initials: K
authors.lastname: Barrow
authors.forename: Kaitlyn
authors.initials: K
authors.lastname: Kieta
authors.forename: Cheryl
authors.initials: C
authors.lastname: Taylor-Howell
authors.forename: Sean
authors.initials: S
authors.lastname: Kearney
authors.forename: Cassandra P
authors.initials: CP
authors.lastname: Smith
authors.forename: Allison
authors.initials: A
authors.lastname: Gibbs
authors.forename: Ann M
authors.initials: AM
authors.lastname: Farese
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: MacVittie
authors.forename: Maureen A
authors.initials: MA
authors.lastname: Kane
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D008055:Lipids / Q000032:analysis*; D055370:Lung Injury / Q000175:diagnosis / Q000378:metabolism* / Q000517:prevention & control*; D008253:Macaca mulatta; D008297:Male; D008665:Metalloporphyrins / Q000008:administration & dosage; D011832:Radiation Injuries / Q000175:diagnosis / Q000378:metabolism* / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D016896:Treatment Outcome
chemical_list: C472668:AEOL 10150; D015415:Biomarkers; D008055:Lipids; D008665:Metalloporphyrins; D011837:Radiation-Protective Agents