title: Young Coconut Juice Supplementation Results in Greater Bone Mass and Bone Formation Indices in Ovariectomized Rats: A Preliminary Study.
abstract: Young coconut juice (Cocos nucifera Linn.) (YCJ) has traditionally been consumed to alleviate symptoms associated with menopause by women in Southeast Asia. The aim of the present study was to determine the effects of YCJ on bone metabolism in ovariectomized rats. Female 10-week-old Wistar rats were randomly assigned to the following 4 groups: Baseline, Sham, Ovx, and Ovx + YCJ (n = 10 rats per group). Rats in the Baseline group were sacrificed immediately, and those in the other groups were subjected to either sham operation (Sham) or bilateral ovariectomy (Ovx and Ovx + YCJ). The Ovx + YCJ rats were administered 5×-concentrated YCJ at a dose of 10 mL/kg body weight per day. Six weeks after surgery, the rats were sacrificed, and indices of bone mass and bone histomorphometry were measured. The bone mineral density of the left femur was significantly higher in the Ovx + YCJ group compared with the Ovx group. In addition, the Ovx + YCJ group showed significantly higher measurements for bone formation rate compared with the Ovx group. These findings suggest that YCJ supplementation has a positive effect on bone metabolism and thus represents a possible intervention to slow the bone loss observed following menopause.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Aichi Medical University, Nagakute, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Morii
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Aichi Medical University, Nagakute, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsushita
authors.affiliation: Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Minami
authors.affiliation: Department of Functional Anatomy, School of Nursing, University of Shizuoka, Shizuoka, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kanazawa
authors.affiliation: Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Thailand.
authors.forename: Sanan
authors.initials: S
authors.lastname: Subhadhirasakul
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Aichi Medical University, Nagakute, Japan.
authors.forename: Kazushi
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Aichi Medical University, Nagakute, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Wakatsuki
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D001851:Bone Diseases, Metabolic / Q000188:drug therapy*; D003059:Cocos / Q000737:chemistry*; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005260:Female; D005269:Femur / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar
keywords: bone histomorphometry; bone mineral density; menopause; rat; young coconut juice