title: Correlation Between RAB27B and p53 Expression and Overall Survival in Pancreatic Cancer.
abstract: OBJECTIVE
  RAB27B is a member of the Rab family GTPases involved in vesicle trafficking, and p53 has recently been implicated in regulating the exosome secretion pathway. Because exosome secretion plays an important role in modulating tumor microenvironment and invasive growth, we hypothesized that RAB27B and p53 expression might be associated with the aggressive behavior in pancreatic ductal adenocarcinoma, one of the most deadly human malignancies.
  
  
  METHODS
  We determined protein expression of RAB27B and p53 in 260 pancreatic tissues (186 malignant and 74 normal or benign) by immunohistochemistry analysis on tissue microarray and their correlation with patients' clinical parameters and overall survival.
  
  
  RESULTS
  We found that a high RAB27B protein expression (RAB27B) was significantly associated with perineural and vascular invasion, as well as distant metastasis. Patients with a high RAB27B expression had significantly poorer overall survival in both univariate and multivariate analyses. A significant correlation between RAB27B and p53 expression was observed.
  
  
  CONCLUSIONS
  Our data indicate that RAB27B expression is an independent prognostic marker for pancreatic ductal adenocarcinoma and suggest that RAB27B-regulated exosome secretion pathway represents a novel therapeutic target in pancreatic cancer.
authors.affiliation: From the *Departments of Interventional Radiology, †General Surgery, ‡Laboratory Medicine, and §Pathology, and ∥Surgical Comprehensive Laboratory, Nantong University Affiliated Hospital, Jiangsu, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Wang
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhu
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
authors.forename: Jianfei
authors.initials: J
authors.lastname: Huang
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis; D021441:Carcinoma, Pancreatic Ductal / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D016019:Survival Analysis; D046888:Tissue Array Analysis; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*; D020691:rab GTP-Binding Proteins / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; D016159:Tumor Suppressor Protein p53; C512960:Rab27B protein, human; D020691:rab GTP-Binding Proteins