title: FOXG1 expression shows correlation with neuronal differentiation in cerebellar development, aggressive phenotype in medulloblastomas, and survival in a xenograft model of medulloblastoma.
abstract: FOXG1 is a transcription factor that interacts with multiple signaling pathways and modulates neuronal differentiation in the telencephalon. Dysregulation of FOXG1 expression has been previously reported in medulloblastoma. In this study, we demonstrate a regional specific expression of FOXG1 and its colocalization with Nestin expression in the premigratory mitotically active (outer) layer of the external granular layer of the cerebellum. An inverse expression of the granular precursor cell markers, Math1 and Musashi1, in the inner nonmitotic migratory layer of the external granular layer and in the internal granular layer was observed. Furthermore, modulation of FOXG1 in the medulloblastoma cell line, DAOY, was associated with the induction of neuronal differentiation markers and significant changes in multiple signaling pathways regulating cell proliferation, differentiation, survival, and apoptosis. Additionally, we observed enhanced survival in intracerebellar mice xenografts injected with DAOY cells bearing shFOXG1 constructs versus shLuciferase construct. Overall, these findings suggest that down-modulation of FOXG1 is a prerequisite for the onset of neuronal differentiation during cerebellar development and that a decrease of FOXG1 in medulloblastoma cells offers a survival advantage in mice. We propose that the disruption of signaling pathways that promote mature neuronal differentiation by overexpressed FOXG1 is a contributing event in the neoplastic transformation of cerebellar stem cells.
authors.affiliation: Department of Pathology and Immunology, Texas Children's Hospital, Baylor College of Medicine, Houston, TX 77030; Department of Pediatrics-Hematology/Oncology, Texas Children's Hospital, Baylor College of Medicine, Houston, TX 77030. Electronic address: aadesina@bcm.edu.
authors.forename: Adekunle M
authors.initials: AM
authors.lastname: Adesina
authors.affiliation: Department of Pathology and Immunology, Texas Children's Hospital, Baylor College of Medicine, Houston, TX 77030.
authors.forename: Bethany L
authors.initials: BL
authors.lastname: Veo
authors.affiliation: Department of Pathology and Immunology, Texas Children's Hospital, Baylor College of Medicine, Houston, TX 77030.
authors.forename: Girard
authors.initials: G
authors.lastname: Courteau
authors.affiliation: Department of Pathology and Immunology, Texas Children's Hospital, Baylor College of Medicine, Houston, TX 77030.
authors.forename: Vidya
authors.initials: V
authors.lastname: Mehta
authors.affiliation: Department of Pathology and Immunology, Texas Children's Hospital, Baylor College of Medicine, Houston, TX 77030.
authors.forename: Xuli
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Pediatrics-Neurology, Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Baylor College of Medicine, Houston, TX 77030.
authors.forename: Kaifang
authors.initials: K
authors.lastname: Pang
authors.affiliation: Department of Pediatrics-Neurology, Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Baylor College of Medicine, Houston, TX 77030.
authors.forename: Zhandong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Pediatrics-Hematology/Oncology, Texas Children's Hospital, Baylor College of Medicine, Houston, TX 77030.
authors.forename: Xiao-Nan
authors.initials: XN
authors.lastname: Li
authors.affiliation: Department of Pathology and Immunology, Texas Children's Hospital, Baylor College of Medicine, Houston, TX 77030.
authors.forename: Lori
authors.initials: L
authors.lastname: Peters
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D002528:Cerebellar Neoplasms / Q000473:pathology*; D002531:Cerebellum / Q000196:embryology; D004195:Disease Models, Animal; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D008527:Medulloblastoma / Q000473:pathology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D058953:Neural Stem Cells / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering; D014161:Transduction, Genetic
chemical_list: C090173:FOXG1 protein, human; D051858:Forkhead Transcription Factors; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering
keywords: Cerebellar development; FOXG1; Medulloblastoma; Neuronal differentiation; Notch signaling; TGFβ signaling; Wnt pathway