title: The influence of various rehydration protocols on biomechanical properties of different acellular tissue matrices.
abstract: OBJECTIVE
  This study evaluated the influence of different rehydration media and time periods on biomechanical and structural properties of different acellular collagen matrices (ACMs).
  
  
  METHODS
  Specimens of three ACMs (mucoderm®, Mucograft®, Dynamatrix®) were rehydrated in saline solution (SS) or human blood for different time periods (5-60 min). ACMs under dry condition served as controls. Biomechanical properties of the ACMs after different rehydration periods were determined by means of tensile testing. ACMs' properties were further characterized using Fourier-transform-infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC).
  
  
  RESULTS
  At dry conditions, mucoderm® presented the highest tensile strength (TS) and Dynamatrix® showed the maximum elastic modulus (EM; p each ≤0.036). Rehydration in SS and blood resulted in significant TS changes of mucoderm® (p each ≤0.05). Concering EM, mucograft® showed significantly decreased values after rehydration in SS compared to Dynamatrix® and mucoderm® after 10 min (p each ≤0.024). mucoderm® hydrated for 5 min in blood displayed nearly double TS and a significantly increased EM after 60 min (p = 0.043) compared to rehydration in SS. TS and EM values of Dynamatrix® and Mucograft® were not altered following rehydration in blood versus SS (p each ≥0.053). FTIR analysis confirmed the recovery of the graft protein backbone with increased rehydration in all samples. DSC measurements revealed that tissue hydration decreased thermal stability of the investigated ACMs.
  
  
  CONCLUSIONS
  Our findings demonstrated that the rehydration protocol affects the biomechanical properties of ACMs.
  
  
  CONCLUSIONS
  Clinicians should be aware of altered handling and mechanical properties of ACMs following different rehydration protocols.
authors.affiliation: Department of Operative Dentistry and Periodontology, University Medical Center, Augustusplatz 2, 55131, Mainz, Germany. Kasaj@gmx.de.
authors.forename: Adrian
authors.initials: A
authors.lastname: Kasaj
authors.affiliation: Division of Periodontology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada.
authors.forename: Liran
authors.initials: L
authors.lastname: Levin
authors.affiliation: Department of Periodontology, Victor Babes University of Medicine and Pharmacology, Timisoara, Romania.
authors.forename: Stefan-Ioan
authors.initials: SI
authors.lastname: Stratul
authors.affiliation: Department of Applied Structure- and Microanalysis, University Medical Center, Mainz, Germany.
authors.forename: Hermann
authors.initials: H
authors.lastname: Götz
authors.affiliation: Private Practice, Forchheim, Goethe University, Frankfurt, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Schlee
authors.affiliation: Department of Operative Dentistry and Periodontology, Federal Armed Forces Hospital, Koblenz, Germany.
authors.forename: Constantin B
authors.initials: CB
authors.lastname: Rütters
authors.affiliation: Deceased; previously Institute of Functional and Clinical Anatomy, University Medical Center, Mainz, Germany.
authors.forename: Moritz A
authors.initials: MA
authors.lastname: Konerding
authors.affiliation: Institute of Functional and Clinical Anatomy, University Medical Center, Mainz, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Ackermann
authors.affiliation: Department of Operative Dentistry and Periodontology, University Medical Center, Augustusplatz 2, 55131, Mainz, Germany.
authors.forename: Brita
authors.initials: B
authors.lastname: Willershausen
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Medical Center, Mainz, Germany.
authors.forename: Andreas M
authors.initials: AM
authors.lastname: Pabst
mesh_terms: D062088:Acellular Dermis; D001696:Biomechanical Phenomena; D002151:Calorimetry / Q000379:methods; D003094:Collagen / Q000737:chemistry*; D055119:Elastic Modulus; D005440:Fluid Therapy / Q000379:methods*; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength
chemical_list: C000623378:Dynamatrix; D008567:Membranes, Artificial; C000623377:Mucoderm; C000623317:Mucograft; D003094:Collagen
keywords: Collagen; Collagen matrix; Graft; Porcine acellular dermis; Tissue regeneration