title: Extending the reservoir of bla IMP-5: the emerging pathogen Acinetobacter bereziniae.
abstract: OBJECTIVE
  Acinetobacter bereziniae clinical relevance is starting to be recognized; however, very few descriptions of its carbapenem resistance currently exist. Here we characterize two carbapenem-resistant A. bereziniae isolates.
  
  
  METHODS
  Isolates were obtained from environmental and clinical samples. Carbapenemases were searched by phenotypic, biochemical and PCR assays. Clonality was studied by ApaI-PFGE and genetic location for carbapenemase genes were assessed by I-CeuI and S1 hybridizations.
  
  
  RESULTS
  Isolates were not clonally related but both produced the 'exclusively Portuguese' IMP-5, with the clinical isolate also producing an OXA-58. The carbapenemase genes were plasmid located.
  
  
  CONCLUSIONS
  Our results emphasize the role of non-baumannii Acinetobacter species as important reservoirs of clinically relevant resistance genes that could also contribute to their emergence as nosocomial pathogens.
authors.affiliation: UCIBIO-REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
authors.forename: Filipa
authors.initials: F
authors.lastname: Grosso
authors.affiliation: UCIBIO-REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.|Escola Superior de Saúde Dr. Lopes Dias, Instituto Politécnico de Castelo Branco, Castelo Branco, Portugal.
authors.forename: Liliana
authors.initials: L
authors.lastname: Silva
authors.affiliation: CEB- Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Clara
authors.initials: C
authors.lastname: Sousa
authors.affiliation: Hospital Geral de Santo António, Porto, Portugal.
authors.forename: Helena
authors.initials: H
authors.lastname: Ramos
authors.affiliation: CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos. Universidade do Porto (CIBIO/UP)/InBio Laboratório Associado, Vairão, Portugal.|Faculdade de Ciências da Universidade do Porto, Departamento de Biologia, Porto, Portugal.|CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Gandra PRD, Portugal.
authors.forename: Sandra
authors.initials: S
authors.lastname: Quinteira
authors.affiliation: UCIBIO-REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
authors.forename: Luísa
authors.initials: L
authors.lastname: Peixe
mesh_terms: D000150:Acinetobacter / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000188:drug therapy* / Q000382:microbiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C534889:beta-lactamase OXA-58, Acinetobacter baumannii; C063912:carbapenemase
keywords: Acinetobacter bereziniae; IMP-5; carbapenem resistance; integron; metallo-β-lactamase; oxacillinase