title: Comparison of Influenza Virus Particle Purification Using Magnetic Sulfated Cellulose Particles with an Established Centrifugation Method for Analytics.
abstract: A method for the purification of influenza virus particles using novel magnetic sulfated cellulose particles is presented and compared to an established centrifugation method for analytics. Therefore, purified influenza A virus particles from adherent and suspension MDCK host cell lines were characterized on the protein level with mass spectrometry to compare the viral and residual host cell proteins. Both methods allowed one to identify all 10 influenza A virus proteins, including low-abundance proteins like the matrix protein 2 and nonstructural protein 1, with a similar impurity level of host cell proteins. Compared to the centrifugation method, use of the novel magnetic sulfated cellulose particles reduced the influenza A virus particle purification time from 3.5 h to 30 min before mass spectrometry analysis.
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems , Sandtorstrasse 1, 39106 Magdeburg, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Serve
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems , Sandtorstrasse 1, 39106 Magdeburg, Germany.
authors.forename: Michael Martin
authors.initials: MM
authors.lastname: Pieler
authors.affiliation: Otto von Guericke University Magdeburg , Universitätsplatz 2, 39106 Magdeburg, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Benndorf
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems , Sandtorstrasse 1, 39106 Magdeburg, Germany.
authors.forename: Erdmann
authors.initials: E
authors.lastname: Rapp
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems , Sandtorstrasse 1, 39106 Magdeburg, Germany.|Otto von Guericke University Magdeburg , Universitätsplatz 2, 39106 Magdeburg, Germany.
authors.forename: Michael Werner
authors.initials: MW
authors.lastname: Wolff
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems , Sandtorstrasse 1, 39106 Magdeburg, Germany.|Otto von Guericke University Magdeburg , Universitätsplatz 2, 39106 Magdeburg, Germany.
authors.forename: Udo
authors.initials: U
authors.lastname: Reichl
mesh_terms: D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D002498:Centrifugation; D006801:Humans; D008280:Magnetics; D009975:Orthomyxoviridae / Q000737:chemistry / Q000302:isolation & purification*; D014771:Virion / Q000737:chemistry / Q000302:isolation & purification*; D014773:Virology / Q000379:methods*
chemical_list: D002482:Cellulose; C020254:cellulose sulfate