title: Densitometric validation and analysis of biomarker β-amyrin in different Acacia species (leaves) grown in Kingdom of Saudi Arabia by high performance thin-layer chromatography.
abstract: Biomarker β-amyrin was analyzed in the leaves of four different Acacia species (A. salicina, A. loreta, A. hamulosa and A. tortilis) grown in Kingdom of Saudi Arabia by a validated HPTLC method. The chromatography was performed on glass-backed silica gel 60 F254 HPTLC plates using solvents toluene: methanol (9:1, v/v) as mobile phase. The developed TLC plate was derivatized with anisaldehyde and scanned at 520 nm. A sharp peak of β-amyrin was found at Rf=0.58±0.01. The r2 and the linear regression equation for β-amyrin was found to be 0.991 and 19.913X+107.803, respectively in the concentration range of 100-800 ng. The percentage of β-amyrin was found to be maximum 2.70% w/w in A. tortilis, 1.85% w/w in A. loreta and 1.80% w/w in A. hamulosa while it was totally absent in A. salicina. This study conceives maiden reporting of quantification of β-amyrin in four different species of Acacia by validated HPTLC method. The developed method for the analysis of β-amyrin was proved to be reproducible by statistical analysis hence it can be employed for further analysis of β-amyrin in plasma, other biological fluids and in finished products available in the market.
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, KSA.
authors.forename: Perwez
authors.initials: P
authors.lastname: Alam
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, KSA.
authors.forename: Mohamed Fahad
authors.initials: MF
authors.lastname: Alajmi
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, KSA.
authors.forename: Nasir Ali
authors.initials: NA
authors.lastname: Siddiqui
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, KSA.
authors.forename: Adnan Jathlan
authors.initials: AJ
authors.lastname: Al-Rehaily
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, KSA.
authors.forename: Hattan
authors.initials: H
authors.lastname: Alharbi
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, KSA.
authors.forename: Omer Ahmed
authors.initials: OA
authors.lastname: Basudan
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, KSA.
authors.forename: Afzal
authors.initials: A
authors.lastname: Hussain
mesh_terms: D000045:Acacia / Q000737:chemistry*; D015415:Biomarkers; D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D009828:Oleanolic Acid; C036380:beta-amyrin