title: Molecular Characterization of LjABCG1, an ATP-Binding Cassette Protein in Lotus japonicus.
abstract: LjABCG1, a full-size ABCG subfamily of ATP-binding cassette proteins of a model legume, Lotus japonicus, was reported as a gene highly expressed during the early stages of nodulation, but have not been characterized in detail. In this study we showed that the induction of LjABCG1 expression was remarkable by methyl jasmonate treatment, and reporter gene experiments indicated that LjABCG1 was strongly expressed in the nodule parenchyma and cell layers adjacent to the root vascular tissue toward the nodule. LjABCG1 was suggested to be localized at the plasma membrane based on the fractionation of microsomal membranes as well as separation via aqueous two-phase partitioning. The physiological functions of LjABCG1 in symbiosis and pathogenesis were analyzed in homologous and heterologous systems. LjABCG1 knock-down L. japonicus plants did not show clear phenotypic differences in nodule formation, and not in defense against Pseudomonas syringae, either. In contrast, when LjABCG1 was expressed in the Arabidopsis pdr8-1 mutant, the penetration frequency of Phytophthora infestans, a potato late blight pathogen, was significantly reduced in LjABCG1/pdr8-1 than in pdr8-1 plants. This finding indicated that LjABCG1, at least partially, complemented the phenotype of pdr8 in Arabidopsis, suggesting the multiple roles of this protein in plant-microbe interactions.
authors.affiliation: Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, 611-0011, Japan.
authors.forename: Akifumi
authors.initials: A
authors.lastname: Sugiyama
authors.affiliation: Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, 611-0011, Japan.
authors.forename: Shoju
authors.initials: S
authors.lastname: Fukuda
authors.affiliation: Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, 611-0011, Japan.
authors.forename: Kojiro
authors.initials: K
authors.lastname: Takanashi
authors.affiliation: Laboratory of Defense in Plant-Pathogen Interactions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Yoshioka
authors.affiliation: Laboratory of Defense in Plant-Pathogen Interactions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshioka
authors.affiliation: Research Institute for Biological Sciences, Okayama, 7549-1, Yoshikawa, Kaga-gun, Okayama, 716-1241, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Narusaka
authors.affiliation: Research Institute for Biological Sciences, Okayama, 7549-1, Yoshikawa, Kaga-gun, Okayama, 716-1241, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Narusaka
authors.affiliation: RIKEN Center for Sustainable Resource Science, 1-7-22, Suehiro, Tsurumi, Yokohama, 230-0045, Japan.
authors.forename: Mikiko
authors.initials: M
authors.lastname: Kojima
authors.affiliation: RIKEN Center for Sustainable Resource Science, 1-7-22, Suehiro, Tsurumi, Yokohama, 230-0045, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sakakibara
authors.affiliation: Laboratory of Natural Medicinal Chemistry, Kobe Pharmaceutical University, Kobe, 658-8558, Japan.
authors.forename: Nobukazu
authors.initials: N
authors.lastname: Shitan
authors.affiliation: Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Japan; Kazusa DNA Research Institute, 2-6-7, Kazusa-Kamatari, Kisarazu, Chiba, 292-0812, Japan.
authors.forename: Shusei
authors.initials: S
authors.lastname: Sato
authors.affiliation: Kazusa DNA Research Institute, 2-6-7, Kazusa-Kamatari, Kisarazu, Chiba, 292-0812, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tabata
authors.affiliation: National Institute for Basic Biology, Nishigonaka 38, Myodaiji, Okazaki, 444-8585, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Kawaguchi
authors.affiliation: Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, 611-0011, Japan.
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Yazaki
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics / Q000382:microbiology; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017343:Genes, Plant; D000070116:Lotus / Q000235:genetics*; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D044224:Pseudomonas syringae / Q000276:immunology; D034622:RNA Interference; D034741:RNA, Small Interfering; D013559:Symbiosis / Q000235:genetics
chemical_list: D018528:ATP-Binding Cassette Transporters; C510424:PDR8 protein, Arabidopsis; D010940:Plant Proteins; D034741:RNA, Small Interfering