title: Unleashing Optics and Optoacoustics for Developmental Biology.
abstract: The past decade marked an optical revolution in biology: an unprecedented number of optical techniques were developed and adopted for biological exploration, demonstrating increasing interest in optical imaging and in vivo interrogations. Optical methods have become faster and have reached nanoscale resolution, and are now complemented by optoacoustic (photoacoustic) methods capable of imaging whole specimens in vivo. Never before were so many optical imaging barriers broken in such a short time-frame: with new approaches to optical microscopy and mesoscopy came an increased ability to image biology at unprecedented speed, resolution, and depth. This review covers the most relevant techniques for imaging in developmental biology, and offers an outlook on the next steps for these technologies and their applications.
authors.affiliation: Department of Bioengineering and Aerospace Engineering, Universidad Carlos III de Madrid, 28911 Madrid, Spain; Experimental Medicine and Surgery Unit, Instituto de Investigación Sanitaria Gregorio Marañón, 28007 Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Ripoll
authors.affiliation: Carl Zeiss AG, Corporate Research and Technology, Carl-Zeiss-Promenade 10, 07745 Jena, Germany; Institute of Biological and Medical Imaging (IBMI), Technische Universität München and Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Koberstein-Schwarz
authors.affiliation: Institute of Biological and Medical Imaging (IBMI), Technische Universität München and Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany. Electronic address: v.ntziachristos@helmholtz-muenchen.de.
authors.forename: V
authors.initials: V
authors.lastname: Ntziachristos
mesh_terms: D000818:Animals; D015509:Developmental Biology / Q000379:methods*; D006801:Humans; D061848:Optical Imaging / Q000379:methods*; D055095:Optics and Photonics / Q000379:methods*; D061088:Photoacoustic Techniques / Q000379:methods*