title: Histological features of oral epithelium in seven animal species: As a reference for selecting animal models.
abstract: Several animals have been used as models for basic and clinical research on oral mucosa. Few studies have focused on the selection of an appropriate animal model. This study aimed to provide histological references for selecting a potential model. Histological features were assessed by exploring 6 morphological characteristics and 2 immunohistochemical markers. The morphological characteristics included keratinization, basal membrane appearance, epithelial thickness, rete ridge length, adjacent rete ridge distance, and regional variation; the immunohistochemical markers included Ki67 (a proliferative marker) and Cytokeratin 19 (CK19; a stemness marker). The histological similarity of each species compared to humans was calculated according to the designated scoring criteria. The results showed that the buccal mucosae from dog and pig were non-keratinized, with similar rete ridge length and distance, compared to that of humans. The dog, rat, and cavy mucosae had analogous gross appearances in the basal membrane. The dog oral mucosae shared similar epithelial thickness with human oral mucosae. Compared to the human mucosa, the dog, pig, rat, and rabbit mucosae exhibited corresponding regional variations. The Ki67-positive cells in human and canine mucosae were predominantly localized in the suprabasal layers, whereas most of the proliferative cells were in the basal layer in other species. CK19 immunoreactivities were detected only in human and canine mucosae. The canine mucosae gained the highest point value (14), whereas the scores for the pig, rat, rabbit, cavy, sheep, and buffalo mucosae were 8, 6, 5, 5, 5, and 2, respectively. The histological variations in the oral epithelium of diverse animal species are considerable; the mucosae from dogs are most similar to human mucosae, implicating its histological basis as an animal model.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Sa
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China; Department of Oral Maxillofacial-Head Neck Oncology, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China.
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Xiong
authors.affiliation: Department of Oral Maxillofacial-Head Neck Oncology, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China.
authors.forename: Tianfu
authors.initials: T
authors.lastname: Wu
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China.
authors.forename: Jincheng
authors.initials: J
authors.lastname: Yang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China; Department of Oral and Maxillofacial Trauma and Plastic Aesthetic Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China. Electronic address: sangang@whu.edu.cn.
authors.forename: Sangang
authors.initials: S
authors.lastname: He
authors.affiliation: Department of Oral Maxillofacial-Head Neck Oncology, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China.
authors.forename: Yifang
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002020:Buffaloes; D004285:Dogs; D006801:Humans; D053539:Keratin-19 / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D023421:Models, Animal; D009061:Mouth Mucosa / Q000033:anatomy & histology* / Q000378:metabolism; D011817:Rabbits; D012377:Rodentia; D012756:Sheep; D013552:Swine
chemical_list: D053539:Keratin-19; D019394:Ki-67 Antigen
keywords: Animal model; Histological features; Oral epithelium; Oral mucosal drug delivery; Tissue engineering