title: Mechanism of QSYQ on anti-apoptosis mediated by different subtypes of cyclooxygenase in AMI induced heart failure rats.
abstract: BACKGROUND
  Qi-shen-yi-qi (QSYQ), one of the most well-known traditional Chinese medicine (TCM) formulas, has been shown to have cardioprotective effects in rats with heart failure (HF) induced by acute myocardial infarction (AMI). However, the mechanisms of its therapeutic effects remain unclear. In this study, we aim to explore the mechanisms of QSYQ in preventing left ventricular remodelling in rats with HF. The anti-apoptosis an anti-inflammation effects of QSYQ were investigated.
  
  
  METHODS
  Sprague-Dawley (SD) rats were randomly divided into 4 groups: sham group, model group, QSYQ treatment group and aspirin group. Heart failure model was induced by ligation of left anterior descending (LAD) coronary artery. 28 days after surgery, hemodynamics were detected. Echocardiography was adopted to evaluate heart function. TUNEL assay was applied to assess myocardial apoptosis rates. Protein expressions of cyclooxygenase1 and 2 (COX1and COX2), Fas ligand (FasL), P53 and MDM2 were measured by western-blot. RT-PCR was applied to detect expressions of our subtype receptors of PGE2 (EP1, 2, 3, and 4).
  
  
  RESULTS
  Ultrasonography showed that EF and FS values decreased significantly and abnormal hemodynamic alterations were observed in model group compared to sham group. These indications illustrated that HF models were successfully induced. Levels of inflammatory cytokines (TNF-α and IL-6) in myocardial tissue were up-regulated in the model group as compared to those in sham group. Western-blot analysis showed that cyclooxygenase 2, which is highly inducible by inflammatory cytokines, increased significantly. Moreover, RT-PCR showed that expressions of EP2 and EP4, which are the receptors of PGE2, were also up-regulated. Increased expressions of apoptotic pathway factors, including P53 and FasL, might be induced by the binding of PGE2 with EP2/4. MDM2, the inhibitor of P53, decreased in model group. TUNEL results manifested that apoptosis rates of myocardial cells increased in the model group. After treatment with QSYQ, expressions of inflammatory factors, including TNF-α, IL-6 and COX2, were reduced. Expressions of EP2 and EP4 receptors also decreased, suggesting that PGE2-mediated apoptosis was inhibited by QSYQ. MDM2 was up-regulated and P53 and FasL in the apoptotic pathway were down-regulated. Apoptosis rates in myocardial tissue in the QSYQ group decreased compared with those in the model group.
  
  
  CONCLUSIONS
  QSYQ exerts cardiac protective efficacy mainly through inhibiting the inflammatory response and down-regulating apoptosis. The anti-inflammatory and anti-apoptosis efficacies of QSYQ are probably achieved by inhibition of COXs-induced P53/FasL pathway. These findings provide experimental evidence for the beneficial effects of QSYQ in the clinical application for treating patients with HF.
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China. jwanghappy@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China. 34451574@qq.com.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China. yuancaobucm@163.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Basic Medical College, Beijing University of Chinese Medicine, Beijing, China. wangqybucm@163.com.
authors.forename: Qiyan
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Basic Medical College, Beijing University of Chinese Medicine, Beijing, China. lhlubucm@163.com.
authors.forename: Linghui
authors.initials: L
authors.lastname: Lu
authors.affiliation: Basic Medical College, Beijing University of Chinese Medicine, Beijing, China. chbucm@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Chang
authors.affiliation: Scientific Research Centre, Beijing University of Chinese Medicine, Beijing, China. yanwubucm@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Scientific Research Centre, Beijing University of Chinese Medicine, Beijing, China. hanjingbucm@sina.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Han
authors.affiliation: Basic Medical College, Beijing University of Chinese Medicine, Beijing, China. wangwei_8@sina.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China. doctupf@163.com.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Tu
authors.affiliation: Basic Medical College, Beijing University of Chinese Medicine, Beijing, China. doctor_wangyong@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051545:Cyclooxygenase 1 / Q000235:genetics / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006333:Heart Failure / Q000188:drug therapy* / Q000201:enzymology / Q000209:etiology / Q000503:physiopathology; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008297:Male; D009203:Myocardial Infarction / Q000150:complications*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D020257:Ventricular Remodeling / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2