title: Anticancer efficacy of allogeneic vaccine modified with progenitor neural cells supernatant in rats with glioma 101.8.
abstract: The aim of the work was to investigate the antitumor efficacy of allogeneic tumor vaccine (ATV) modified with rat progenitor neural cell supernatant (RPNS) in rats with glioma 101.8.
  
  
  METHODS
  The study was performed on 74 white random-bred rats. ATV was developed on the basis of glioma 101.8 cell suspension modified with RPNS (0.02 or 0.10 mg/ml). RPNS was prepared from suspension made from whole rat brain tissue on 14(th) (E14) day of gestation. Model of brain glioma 101.8 was reproduced by intracerebral injection of glioma 101.8 cell suspension. ATV was injected intraperitoneally in a volume of 0.2 ml per animal once on the 10(th) day after tumor transplantation. For survival analysis Kaplan - Mayer multiple assessments method was used. Cytotoxic activity of rat lymphocytes (effector cells) was evaluated in MTT-colorimetric test by determining the state of mitochondrial dehydrogenase enzymes in target cells (allogeneic glioma 101.8 cells).
  
  
  RESULTS
  Intraperitoneal administration of ATV modified with 0.10 mg/ml RPNS significantly increased mean survival time and median survival of glioma-bearing rats compared with unvaccinated group (MST (19.9 ± 2.4), 21.4 days; versus MST (14.6 ± 2.8); 14 days; p = 0.0002, Gehan's - Wilcoxon test). Intraperitoneal administration of ATV modified with 0.10 mg/ml RPNS resulted in increased cytotoxic activity of immune cells of rats with glioma in vitro compared with this index in unvaccinated group (p = 0.026, U-Mann - Whitney test).
  
  
  CONCLUSIONS
  Antitumor effect of vaccination with RPNS-modified ATV is realized via increased cytotoxic activity of immune cells, what could be used for further optimization of whole tumor cell vaccine.
authors.affiliation: SI "A.P. Romodanov Institute of Neurosurgery of the NAMS of Ukraine", Kyiv 04050, Ukraine.
authors.forename: L D
authors.initials: LD
authors.lastname: Liubich
authors.affiliation: SI "A.P. Romodanov Institute of Neurosurgery of the NAMS of Ukraine", Kyiv 04050, Ukraine.
authors.forename: M I
authors.initials: MI
authors.lastname: Lisyany
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000276:immunology* / Q000401:mortality / Q000473:pathology* / Q000628:therapy; D019496:Cancer Vaccines / Q000276:immunology*; D017077:Culture Media, Conditioned; D003602:Cytotoxicity, Immunologic; D005910:Glioma / Q000276:immunology* / Q000401:mortality / Q000473:pathology* / Q000628:therapy; D006801:Humans; D008297:Male; D058953:Neural Stem Cells / Q000378:metabolism*; D051381:Rats; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D019496:Cancer Vaccines; D017077:Culture Media, Conditioned