title: Ovarian Transcriptome Analysis of Portunus trituberculatus Provides Insights into Genes Expressed during Phase III and IV Development.
abstract: Enhancing the production of aquatic animals is crucial for fishery management and aquaculture applications. Ovaries are specialized tissues that play critical roles in producing oocytes and hormones. Significant biochemical changes take place during the sexual maturation of Portunus trituberculatus, but the genetics of this process has not been extensively studied. Transcriptome sequencing can be used to determine gene expression changes within specific periods. In the current study, we used transcriptome sequencing to produce a comprehensive transcript dataset for the ovarian development of P. trituberculatus. Approximately 100 million sequencing reads were generated, and 126,075 transcripts were assembled. Functional annotation of the obtained transcripts revealed important pathways in ovarian development, such as those involving the vitellogenin gene. Also, we performed deep sequencing of ovaries in phases III and IV of sexual maturation in P. trituberculatus. Differential analysis of gene expression identified 506 significantly differentially expressed genes, which belong to 20 pathway, transporters, development, transcription factors, metabolism of other amino acids, carbohydrate and lipid, solute carrier family members, and enzymes. Taken together, our study provides the first comprehensive transcriptomic resource for P. trituberculatus ovaries, which will strengthen understanding of the molecular mechanisms underlying the sexual maturation process and advance molecular nutritional studies of P. trituberculatus.
authors.affiliation: Fisheries College of Zhejiang Ocean University, Zhoushan, China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Fisheries College of Zhejiang Ocean University, Zhoushan, China.
authors.forename: Jiteng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Fisheries College of Zhejiang Ocean University, Zhoushan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Han
authors.affiliation: Annoroad Gene Technology Co., Ltd, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Marine Science College of Ningbo University, Ningbo, China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Immunobiology, Jinan University, Guangzhou, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Marine Science College of Ningbo University, Ningbo, China.
authors.forename: Changkao
authors.initials: C
authors.lastname: Mu
authors.affiliation: Marine Science College of Ningbo University, Ningbo, China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Li
authors.affiliation: Marine Fisheries Research Institute of Zhejiang Province, Zhoushan, China.
authors.forename: Fangping
authors.initials: F
authors.lastname: Yu
authors.affiliation: Marine Fisheries Research Institute of Zhejiang Province, Zhoushan, China.
authors.forename: Huilai
authors.initials: H
authors.lastname: Shi
mesh_terms: D000818:Animals; D003445:Crustacea / Q000235:genetics* / Q000254:growth & development; D005260:Female; D010053:Ovary / Q000378:metabolism*; D059467:Transcriptome