title: Delivery of liquorice extract by liposomes and hyalurosomes to protect the skin against oxidative stress injuries.
abstract: Liquorice extract, obtained by percolation in ethanol of Glycyrrhiza glabra L. roots, was incorporated in liposomes and hyalurosomes, new phospholipid-sodium hyaluronate vesicles, and their protective effect against oxidative stress skin damages was probed. As a comparison, raw glycyrrhizin was also tested. All the vesicles were small in size (≤ 100 nm), with a highly negative zeta potential ensuring long-term stability, and able to incorporate a high amount of the extract. In vitro tests showed that the liquorice extract loaded in vesicles was able to scavenge DPPH free radical (80% inhibition) and to protect 3T3 fibroblasts against H2O2-induced oxidative stress, restoring the normal conditions. By contrast, glycyrrhizin showed poor antioxidant activity, and was not able to efficiently counteract the oxidative effect of H2O2. In addition, the incorporation of the liquorice extract into the vesicular systems promoted the proliferation and migration of 3T3 fibroblasts, favouring the closure of the scratched area. In vivo anti-inflammatory tests on mice confirmed the ability of the proposed nanosystems to improve the local efficacy of the extract, favouring the re-epitelization process.
authors.affiliation: Department Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy.
authors.forename: Ines
authors.initials: I
authors.lastname: Castangia
authors.affiliation: Department Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Caddeo
authors.affiliation: Department Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy. Electronic address: mlmanca@unica.it.
authors.forename: Maria Letizia
authors.initials: ML
authors.lastname: Manca
authors.affiliation: Department Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Casu
authors.affiliation: Department Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy; Department of Pharmacy and Pharmaceutical Technology, University of Valencia, Valencia, Spain.
authors.forename: Ana Catalan
authors.initials: AC
authors.lastname: Latorre
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, University of Valencia, Valencia, Spain; Instituto de Reconocimiento Molecular y Desarrollo Tecnológico, Centro Mixto Universidad Politécnica de Valencia-Universidad de Valencia, Valencia, Spain.
authors.forename: Octavio
authors.initials: O
authors.lastname: Díez-Sales
authors.affiliation: Department of Pathology, University of Valencia, Valencia, Spain.
authors.forename: Amparo
authors.initials: A
authors.lastname: Ruiz-Saurí
authors.affiliation: Department Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy.
authors.forename: Gianluigi
authors.initials: G
authors.lastname: Bacchetta
authors.affiliation: Department Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Fadda
authors.affiliation: Department Scienze della Vita e dell'Ambiente, University of Cagliari, Cagliari, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Manconi
mesh_terms: D016475:3T3 Cells; D000287:Administration, Topical; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D004355:Drug Stability; D004487:Edema / Q000188:drug therapy; D005260:Female; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D006035:Glycyrrhiza / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D008081:Liposomes; D008422:Materials Testing; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots / Q000737:chemistry; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D004337:Drug Carriers; D016166:Free Radical Scavengers; D008081:Liposomes; D010936:Plant Extracts; D006820:Hyaluronic Acid
keywords: Fibroblasts; Glycyrrhizin; Liquorice extract; Natural antioxidant; Phospholipid vesicles; Sodium hyaluronate