title: TRPM2 protects against tissue damage following oxidative stress and ischaemia-reperfusion.
abstract: TRPM channels are a subgroup of the transient receptor potential (TRP) channel superfamily whose members have important roles in cell proliferation and survival. TRPM2, the second subfamily member to be cloned, is expressed in many tissues including brain, heart, vasculature and haematopoietic cells. TRPM2 is activated by oxidative stress and several other extracellular signals including tumour necrosis factor α (TNF-α) and amyloid β-peptide, which increase production of ADP-ribose (ADPR). ADPR binds to the TRPM2 C-terminal NUDT9-H domain, activating the channel. Early studies support the paradigm that TRPM2 activation induces cell death by sustained Ca(2+) influx or by enhancing cytokine production, aggravating inflammation and tissue injury. However, more recent data show that for a number of physiological processes, TRPM2 is protective. TRPM2 protects lungs from endotoxin-induced injury by reducing reactive oxygen species (ROS) production by phagocytes. It protects hearts from oxidative damage after ischaemia-reperfusion or hypoxia-reoxygenation by maintaining better mitochondrial bioenergetics and by decreasing ROS. Sustained Ca(2+) entry through TRPM2 is required to maintain cellular bioenergetics and protect against hypoxia-reoxygenation injury. TRPM2 also protects neuroblastoma from moderate oxidative stress by decreasing ROS through increased levels of forkhead box transcription factor 3a (FOXO3a) and a downstream effector, superoxide dismutase 2. TRPM2 is important for tumour growth and cell survival through modulation of hypoxia-inducible transcription factor expression, mitochondrial function and mitophagy. These findings in cardiac ischaemia and in neuroblastoma suggest that TRPM2 has a basic role in sustaining mitochondrial function and in cell survival that applies to a number of physiological systems and pathophysiological processes including ischaemia-reperfusion injury.
authors.affiliation: Departments of Pediatrics and Biochemistry & Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Miller
authors.affiliation: Center of Translational Medicine and Department of Medicine, Temple University School of Medicine, Philadelphia, PA, 19140, USA.
authors.forename: Joseph Y
authors.initials: JY
authors.lastname: Cheung
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival; D006801:Humans; D007674:Kidney Diseases / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology*; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000502:physiology; D009369:Neoplasms / Q000378:metabolism; D018384:Oxidative Stress; D050053:TRPM Cation Channels / Q000378:metabolism / Q000502:physiology*
chemical_list: D050053:TRPM Cation Channels; C121934:TRPM2 protein, human; D002118:Calcium