title: Exploring the Functional Disorder and Corresponding Key Transcription Factors in Intraductal Papillary Mucinous Neoplasms Progression.
abstract: This study has analyzed the gene expression patterns of an IPMN microarray dataset including normal pancreatic ductal tissue (NT), intraductal papillary mucinous adenoma (IPMA), intraductal papillary mucinous carcinoma (IPMC), and invasive ductal carcinoma (IDC) samples. And eight clusters of differentially expressed genes (DEGs) with similar expression pattern were detected by k-means clustering. Then a survey map of functional disorder in IPMN progression was established by functional enrichment analysis of these clusters. In addition, transcription factors (TFs) enrichment analysis was used to detect the key TFs in each cluster of DEGs, and three TFs (FLI1, ERG, and ESR1) were found to significantly regulate DEGs in cluster 1, and expression of these three TFs was validated by qRT-PCR. All these results indicated that these three TFs might play key roles in the early stages of IPMN progression.
authors.affiliation: Department of Oncology, Tianjin Third Central Hospital, Tianjin 300179, China ; Key Laboratory of Artificial Cell Institute of Hepatobiliary Disease, Tianjin Third Central Hospital, Tianjin 300179, China.
authors.forename: Guiying
authors.initials: G
authors.lastname: Bai
authors.affiliation: Department of Oncology, Tianjin Third Central Hospital, Tianjin 300179, China.
authors.forename: Chenxuan
authors.initials: C
authors.lastname: Wu
authors.affiliation: Key Laboratory of Artificial Cell Institute of Hepatobiliary Disease, Tianjin Third Central Hospital, Tianjin 300179, China.
authors.forename: Yingtang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Hepatobiliary Surgery, Tianjin Third Central Hospital, Tianjin 300179, China.
authors.forename: Guiming
authors.initials: G
authors.lastname: Shu