title: Effects of a Particular Heptapeptide on the IFN-α-Sensitive CML Cells.
abstract: Using the phage display biopanning technique, we have previously identified a heptapeptide KLWVIPQ which specifically binds to the surface of the IFN-α-sensitive but not the IFN-α-resistant CML cells. The effects of this heptapeptide on the IFN-α-sensitive CML cells were investigated in the present study. IFN-α-sensitive KT-1/A3 and IFN-α-resistant KT-1/A3R CML cells were transfected by pEGFP-KLWVIPQ expression vector and/or induced by IFN-α. WST-1 cell proliferation assay, flow cytometry, and western blotting were performed to determine the effects of this heptapeptide and/or IFN-α on CML cells. The viability of the KT-1/A3 cells was inhibited and apoptosis was induced by either expression of the heptapeptide KLWVIPQ or IFN-α treatment with concurrent upregulation of P53 and downregulation of P210(bcr/abl). However, these effects were not observed in the IFN-α-resistant KT-1/A3R cells. These results suggest that the heptapeptide KLWVIPQ shares a similar mechanism with IFN-α in the regulation of CML cell growth and apoptosis, implying that the heptapeptide KLWVIPQ could be a novel target to go further into mechanisms of IFN-α sensitivity and/or resistance in CML.
authors.affiliation: Department of Biochemistry, School of Life Sciences and The State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410013, China.
authors.forename: Fu-Lan
authors.initials: FL
authors.lastname: Yang
authors.affiliation: Department of Urology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
authors.forename: Fang-Zhi
authors.initials: FZ
authors.lastname: Chen
authors.affiliation: Department of Biochemistry, School of Life Sciences and The State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410013, China.
authors.forename: Xin-Xing
authors.initials: XX
authors.lastname: Wan
authors.affiliation: Department of Biochemistry, School of Life Sciences and The State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410013, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Biochemistry, School of Life Sciences and The State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410013, China.
authors.forename: Mei-Juan
authors.initials: MJ
authors.lastname: Zhou
authors.affiliation: Department of Biochemistry, School of Life Sciences and The State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410013, China.
authors.forename: Han-Chun
authors.initials: HC
authors.lastname: Chen
authors.affiliation: Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou, Sichuan 646000, China.
authors.forename: Jun-Jiang
authors.initials: JJ
authors.lastname: Fu
authors.affiliation: Department of Biochemistry/Molecular Biology, Philadelphia College of Osteopathic Medicine, Philadelphia, PA 19131, USA.
authors.forename: Dian-Zheng
authors.initials: DZ
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D005822:Genetic Vectors / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000473:pathology*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D010957:Plasmids / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D016898:Interferon-alpha; D009842:Oligopeptides; D016159:Tumor Suppressor Protein p53; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins