title: Changes in the activities of starch metabolism enzymes in rice grains in response to elevated CO2 concentration.
abstract: The global atmospheric CO(2) concentration is currently (2012) 393.1 μmol mol(-1), an increase of approximately 42 % over pre-industrial levels. In order to understand the responses of metabolic enzymes to elevated CO(2) concentrations, an experiment was conducted using the Free Air CO(2) Enrichment (FACE )system. Two conventional japonica rice varieties (Oryza sativa L. ssp. japonica) grown in North China, Songjing 9 and Daohuaxiang 2, were used in this study. The activities of ADPG pyrophosphorylase, soluble and granule-bound starch synthases, and soluble and granule-bound starch branching enzymes were measured in rice grains, and the effects of elevated CO(2) on the amylose and protein contents of the grains were analyzed. The results showed that elevated CO(2) levels significantly increased the activity of ADPG pyrophosphorylase at day 8, 24, and 40 after flower, with maximum increases of 56.67 % for Songjing 9 and 21.31 % for Daohuaxiang 2. Similarly, the activities of starch synthesis enzymes increased significantly from the day 24 after flower to the day 40 after flower, with maximum increases of 36.81 % for Songjing 9 and 66.67 % for Daohuaxiang 2 in soluble starch synthase (SSS), and 25.00 % for Songjing 9 and 36.44 % for Daohuaxiang 2 in granule-bound starch synthase (GBSS), respectively. The elevated CO(2) concentration significantly increased the activity of soluble starch branching enzyme (SSBE) at day 16, 32, and 40 after flower, and also significantly increased the activity of granule-bound starch branching enzyme (GBSBE) at day 8, 32, and 40 after flower. The elevated CO(2) concentration increased the peak values of enzyme activity, and the timing of the activity peaks for SSS and GBSBE were earlier in Songjing 9 than in Daohuaxiang 2. There were obvious differences in developmental stages between the two varieties of rice, which indicated that the elevated CO(2) concentration increased enzyme activity expression and starch synthesis, affecting the final contents of starch and protein in the rice grains. Our results will provide a foundation for understanding the physiological mechanisms of rice yield under elevated atmospheric CO(2) concentrations.
authors.affiliation: College of Agronomy, Shenyang Agricultural University, Shenyang, 110161, China. xly0910@163.com.
authors.forename: Li-Yong
authors.initials: LY
authors.lastname: Xie
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Er-Da
authors.initials: ED
authors.lastname: Lin
authors.affiliation: College of Agronomy, Shenyang Agricultural University, Shenyang, 110161, China.
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Zhao
authors.affiliation: College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, 163319, China.
authors.forename: Yong-Xiang
authors.initials: YX
authors.lastname: Feng
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000378:metabolism*; D000688:Amylose / Q000378:metabolism; D002245:Carbon Dioxide / Q000494:pharmacology*; D051599:Glucose-1-Phosphate Adenylyltransferase / Q000378:metabolism*; D012275:Oryza / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D013213:Starch / Q000378:metabolism; D013214:Starch Synthase / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D002245:Carbon Dioxide; D013213:Starch; D000688:Amylose; D015061:1,4-alpha-Glucan Branching Enzyme; D013214:Starch Synthase; D051599:Glucose-1-Phosphate Adenylyltransferase
keywords: ADPG pyrophosphorylase enzyme; Enzyme activity; FACE; Metabolic enzyme; Rice; Starch branching enzyme; Starch synthesis enzyme