title: Emulating human microcapillaries in a multi-organ-chip platform.
abstract: Current microfluidic chip-based tissue culture systems lack a capillary endothelial vessel system, which would enable perfusion with blood. We utilise spatial cell cultures to populate a perfused multi-organ-chip platform-a microfluidic device recently introduced for substance testing. Complete biological vascularization of such culture systems is vital to properly emulate physiological tissue behaviour. In this study, we incorporated a fibrin scaffold into the two-organ-chip design. Herein, adipose-derived stromal cells (ASCs) directed human umbilical vein endothelial cells (HUVECs) to organise into tube-like structures. The ASCs induced tube formation of HUVECs in static and dynamic conditions. The replacement of full medium enriched with growth factors and foetal calf serum with basal medium resulted in viable cells with similar gene expression profiles. We regard this as a prerequisite for studies with organ constructs that have a need for a different medium formulation. Furthermore, we here address stability issues of the fibrin gel and fibrin composition for optimal microvessel formation.
authors.affiliation: Technische Universität Berlin, Medical Biotechnology, TIB 4/4-2, Gustav-Meyer-Allee 25, 13355 Berlin, Germany; TissUse GmbH, Markgrafenstraße 18, 15528 Spreenhagen, Germany. Electronic address: t.hasenberg@tissuse.com.
authors.forename: Tobias
authors.initials: T
authors.lastname: Hasenberg
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstraße 13, 1200 Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria. Electronic address: Severin.Muehleder@trauma.lbg.ac.at.
authors.forename: Severin
authors.initials: S
authors.lastname: Mühleder
authors.affiliation: Technische Universität Berlin, Medical Biotechnology, TIB 4/4-2, Gustav-Meyer-Allee 25, 13355 Berlin, Germany; TissUse GmbH, Markgrafenstraße 18, 15528 Spreenhagen, Germany. Electronic address: andrea.dotzler@tissuse.com.
authors.forename: Andrea
authors.initials: A
authors.lastname: Dotzler
authors.affiliation: Technische Universität Berlin, Medical Biotechnology, TIB 4/4-2, Gustav-Meyer-Allee 25, 13355 Berlin, Germany. Electronic address: sophie.bauer@tu-berlin.de.
authors.forename: Sophie
authors.initials: S
authors.lastname: Bauer
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstraße 13, 1200 Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria. Electronic address: Krystyna.Labuda@trauma.lbg.ac.at.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Labuda
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstraße 13, 1200 Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria. Electronic address: wolfgang.holnthoner@trauma.lbg.ac.at.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Holnthoner
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstraße 13, 1200 Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria. Electronic address: office@trauma.lbg.ac.at.
authors.forename: Heinz
authors.initials: H
authors.lastname: Redl
authors.affiliation: Technische Universität Berlin, Medical Biotechnology, TIB 4/4-2, Gustav-Meyer-Allee 25, 13355 Berlin, Germany. Electronic address: roland.lauster@tu-berlin.de.
authors.forename: Roland
authors.initials: R
authors.lastname: Lauster
authors.affiliation: Technische Universität Berlin, Medical Biotechnology, TIB 4/4-2, Gustav-Meyer-Allee 25, 13355 Berlin, Germany; TissUse GmbH, Markgrafenstraße 18, 15528 Spreenhagen, Germany. Electronic address: uwe.marx@tissuse.com.
authors.forename: Uwe
authors.initials: U
authors.lastname: Marx
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D002196:Capillaries / Q000502:physiology*; D018929:Cell Culture Techniques; D018920:Coculture Techniques; D003470:Culture Media / Q000494:pharmacology; D005337:Fibrin / Q000494:pharmacology; D005782:Gels / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D044085:Microfluidics / Q000379:methods*; D010477:Perfusion; D016133:Polymerase Chain Reaction; D012212:Rheology / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D059467:Transcriptome / Q000235:genetics
chemical_list: D003470:Culture Media; D005782:Gels; D049452:Green Fluorescent Proteins; D005337:Fibrin
keywords: Co-culture models; Fibrin scaffold; Multi-organ-chip; Tissue engineering; Vasculature; Vasculogenesis