title: Characterization of modified proteins in plasma from a subtype of schizophrenia based on carbonyl stress: Protein carbonyl is a possible biomarker of psychiatric disorders.
abstract: Although it's well known that protein carbonyl (PCO) and advanced glycation end-products (AGEs) levels are elevated in plasma from patients with renal dysfunction, we recently identified patients who had no renal dysfunction but possessed high levels of plasma pentosidine (PEN), which is an AGEs, and low vitamin B6 levels in serum. In this study, we investigated the status of carbonyl stress to characterize the subtype of schizophrenia. When plasma samples were subjected to Western blot analysis for various AGEs, clear differences were only observed with the anti-PEN antibody in the plasma from schizophrenic patients. Moreover, we determined the formation of protein carbonyl (PCO), a typical indicator of carbonyl stress, occurred prior to the accumulation of PEN in the plasma of schizophrenic patients. PCO levels in the plasma from schizophrenic patients were significantly higher than that from healthy subjects. Western blots analysis clearly showed that albumin and IgG were markedly carbonylated in the plasma of some patients. Thus, PCOs may be a novel marker of carbonyl stress-type schizophrenia in addition to albumin containing PEN structure.
authors.affiliation: Department of Analytical Biohemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Koike
authors.affiliation: Department of Analytical Biohemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
authors.forename: Tasuku
authors.initials: T
authors.lastname: Kayama
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya, Tokyo 156-8506, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Arai
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya, Tokyo 156-8506, Japan.
authors.forename: Yasue
authors.initials: Y
authors.lastname: Horiuchi
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya, Tokyo 156-8506, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kobori
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya, Tokyo 156-8506, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Miyashita
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya, Tokyo 156-8506, Japan.
authors.forename: Masanari
authors.initials: M
authors.lastname: Itokawa
authors.affiliation: Department of Analytical Biohemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan. Electronic address: yo@my-pharm.ac.jp.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ogasawara
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001120:Arginine / Q000031:analogs & derivatives / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D017127:Glycation End Products, Advanced / Q000097:blood; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000737:chemistry; D008239:Lysine / Q000031:analogs & derivatives / Q000097:blood; D008297:Male; D008875:Middle Aged; D009952:Ornithine / Q000031:analogs & derivatives / Q000097:blood; D050050:Protein Carbonylation; D011743:Pyrimidines / Q000097:blood; D012559:Schizophrenia / Q000097:blood* / Q000150:complications / Q000175:diagnosis; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism; D025101:Vitamin B 6 / Q000097:blood; D026681:Vitamin B 6 Deficiency / Q000097:blood* / Q000150:complications / Q000175:diagnosis
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D001798:Blood Proteins; D017127:Glycation End Products, Advanced; D007074:Immunoglobulin G; D011743:Pyrimidines; D012709:Serum Albumin; C107313:argpyrimidine; D025101:Vitamin B 6; D001120:Arginine; C062187:pentosidine; D009952:Ornithine; D008239:Lysine
keywords: Albumin; Carbonyl stress; Immunoglobulin; Pentosidine; Protein carbonylation; Schizophrenia; Vitamin B(6)