title: Biomass and lipid enhancement in Ankistrodesmus sp. cultured with reused and minimal nutrients media.
abstract: Microalgae are a promising feedstock for biofuel production. Lipid content in microalgae could be enhanced under nutrient depletion. This work investigated the effect of the nutrient on lipid accumulation in Ankistrodesmus sp. culture. Batch cultures were carried out using fresh BG11 medium, and after the harvest, the medium was reused for the next culture; this method was repeated two times. The maximum lipid productivity of 29.75 mg L(-1) day(-1) was obtained from the culture with the second reuse medium. In continuous cultures, Ankistrodesmus sp. was cultured in both fresh and modified BG11 mediums. The modified BG11 medium was adjusted to resemble the content of the first reuse medium. As a comparison between batch and continuous cultures, it was proven that the productivity in the continuous culture was better than in the batch, where the achievable maximum biomass and lipid were 188.30 and 38.32 mg L(-1) day(-1). The maximum lipid content of 34.22% was obtained from the continuous culture at a dilution rate of 0.08 day(-1), whereas the maximum saturated and unsaturated fatty acid productivities of 79.96 and 104.54 mg L(-1) day(-1) were obtained at a dilution rate of 0.16 day(-1.)
authors.affiliation: a The Joint Graduate School of Energy and Environment , King Mongkut's University of Technology Thonburi , Thung Khru , Bangkok , Thailand.
authors.forename: Katesuda
authors.initials: K
authors.lastname: Sukkrom
authors.affiliation: b Pilot Plant Development and Training Institute , King Mongkut's University of Technology Thonburi , Bangkhuntien , Bangkok , Thailand.
authors.forename: Boosya
authors.initials: B
authors.lastname: Bunnag
authors.affiliation: c Center of Excellence for Marine Biotechnology, Department of Marine Science , Chulalongkorn University , Bangkok , Thailand.
authors.forename: Sorawit
authors.initials: S
authors.lastname: Powtongsook
authors.affiliation: b Pilot Plant Development and Training Institute , King Mongkut's University of Technology Thonburi , Bangkhuntien , Bangkok , Thailand.
authors.forename: Wipawan
authors.initials: W
authors.lastname: Siangdung
authors.affiliation: d Department of Chemical Engineering, Faculty of Engineering , Chulalongkorn University , Bangkok , Thailand.
authors.forename: Prasert
authors.initials: P
authors.lastname: Pavasant
mesh_terms: D018533:Biomass; D000460:Chlorophyta / Q000378:metabolism*; D003470:Culture Media
chemical_list: D003470:Culture Media
keywords: Cultivation; economics; microalgae; modified nutrients; photobioreactor