title: Chemomodulatory Potential of Flaxseed Oil Against DMBA/Croton Oil-Induced Skin Carcinogenesis in Mice.
abstract: The present study was conducted to evaluate the potential of flaxseed oil to prevent chemically induced skin cancer in mice. Cancer was induced on 2-stage skin carcinogenesis model by single topical application of 7,12 dimethylbenz [a]anthracene (DMBA), as, initiator, and two weeks later it was promoted by croton oil treatment thrice a week on the dorsal surface of mice for 16 weeks. Flaxseed oil (FSO; 100µL/animal/d) was orally administered 1 week before and 1 week after DMBA application (Peri-initiation stage). The animals of the FSO-administered group showed a significant reduction in tumor incidence (76.67%), cumulative number of tumors (37), tumor yield (3.7), and tumor burden (4.81) when compared with the carcinogen-treated control animals. Biochemical parameters in skin and liver tissue such as LPO and phase I enzymes were significantly (P < .01) reduced in the FSO-treated experimental group, whereas the phase II enzymes (GST, DT-diaphorase) and antioxidant parameters (GSH, GPx, SOD, catalase, and vitamin C) exhibited a significant (P < .01) elevation when compared with the animals of the carcinogen-treated control group. Histopathological alterations in the carcinogen-treated control animals were also observed in the form of epidermal hyperplasia, keratinized pearl formation, and acanthosis in skin and tumors, whereas these were found to be reduced after FSO administration. The results of the present study demonstrate that the oral administration of FSO has the potential to modulate the levels of LPO, antioxidants, and detoxification enzymes in the DMBA-croton oil-induced skin carcinogenesis in mice.
authors.affiliation: University of Rajasthan, Jaipur, India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Sharma
authors.affiliation: University of Rajasthan, Jaipur, India.
authors.forename: Ritu
authors.initials: R
authors.lastname: Singh
authors.affiliation: University of Rajasthan, Jaipur, India pkgoyal2002@gmail.com.
authors.forename: P K
authors.initials: PK
authors.lastname: Goyal
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000009:adverse effects*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D063646:Carcinogenesis / Q000187:drug effects*; D002273:Carcinogens / Q000008:administration & dosage; D002374:Catalase / Q000378:metabolism; D003436:Croton Oil / Q000009:adverse effects*; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D008043:Linseed Oil / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002273:Carcinogens; D010936:Plant Extracts; D015127:9,10-Dimethyl-1,2-benzanthracene; D008043:Linseed Oil; D003436:Croton Oil; D002374:Catalase; D016660:NAD(P)H Dehydrogenase (Quinone); D005978:Glutathione
keywords: antioxidants; chemoprevention; lipid peroxidation; phase I and phase II enzymes; skin carcinogenesis; α-linolenic acid