title: Antioxidant activity of a new phenolic glycoside from Lagenaria siceraria Stand. fruits.
abstract: The antioxidant properties of different extracts of Lagenaria siceraria (bottle gourd) fruit were evaluated. In the process, a new phenolic glycoside (E)-4-hydroxymethyl-phenyl-6-O-caffeoyl-β-d-glucopyranoside (1) was isolated and identified together with 1-(2-hydroxy-4-hydroxymethyl)-phenyl-6-O-caffeoyl-β-d-gluco-pyranoside (2), protocatechuic acid (3), gallic acid (4), caffeic acid (5) and 3,4-dimethoxy cinnamic acid (6). Their structures were elucidated by extensive NMR experiments including (1)H-(1)H (COSY) and (1)H-(13)C (HMQC and HMBC) spectroscopy and chemical evidences. The antioxidant potential of the compound 1 and 2 was tested in different in vitro assay systems such as free radical scavenging assay, 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay, superoxide scavenging activity, reducing power assay and linoleic acid peroxidation assay.
authors.affiliation: Department of Natural Products, National Institute of Pharmaceutical Education and Research, Sector 67, SAS Nagar, Mohali 160 062, Punjab, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Mohan
authors.affiliation: Department of Natural Products, National Institute of Pharmaceutical Education and Research, Sector 67, SAS Nagar, Mohali 160 062, Punjab, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Birari
authors.affiliation: Department of Natural Products, National Institute of Pharmaceutical Education and Research, Sector 67, SAS Nagar, Mohali 160 062, Punjab, India.
authors.forename: Aniket
authors.initials: A
authors.lastname: Karmase
authors.affiliation: Department of Natural Products, National Institute of Pharmaceutical Education and Research, Sector 67, SAS Nagar, Mohali 160 062, Punjab, India.
authors.forename: Sneha
authors.initials: S
authors.lastname: Jagtap
authors.affiliation: Department of Natural Products, National Institute of Pharmaceutical Education and Research, Sector 67, SAS Nagar, Mohali 160 062, Punjab, India. Electronic address: kkbhutani@niper.ac.in.
authors.forename: Kamlesh Kumar
authors.initials: KK
authors.lastname: Bhutani
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D019662:Cucurbitaceae / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D017382:Reactive Oxygen Species
chemical_list: D000975:Antioxidants; D006027:Glycosides; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species
keywords: HMBC; Lagenaria siceraria; NMR; Phenolic glycoside