title: A Periosteum-Inspired 3D Hydrogel-Bioceramic Composite for Enhanced Bone Regeneration .
abstract: A 3D injectable hydrogel-bioceramic composite consisting of gelatin-3-(4-hydroxyphenyl) propionic acid (Gtn-HPA) and carboxymethyl cellulose-tyramine (CMC-Tyr), incorporated with fish scale-derived calcium phosphate (CaP), is developed for bone applications. The hydrogel-bioceramic composite has significantly improved the elastic modulus compared to the non-filled hydrogel, of which the addition of 10 w/v% CaP showed zero order fluorescein isothiocyanate (FITC)-dextran release profile and a significantly higher proliferation rate of encapsulated cells. All the samples promote the nucleation and growth of CaP minerals when exposed to 1× SBF. Overall, the hydrogel-bioceramic composite with 10 w/v% CaP can potentially be used as a periosteum-mimicking membrane to facilitate bone regeneration.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Singapore.
authors.forename: Yong Yao
authors.initials: YY
authors.lastname: Chun
authors.affiliation: Residues and Resource Reclamation Centre (R3C), Nanyang Environment and Water Research Institute (NEWRI), Nanyang Technological University, 1 Cleantech Loop, Singapore, 637141, Singapore.|Interdisciplinary Graduate School, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.
authors.forename: Jun Kit
authors.initials: JK
authors.lastname: Wang
authors.affiliation: School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.|Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, A*STAR, Singapore, 138673, Singapore.|KK Research Centre, KK Women's and Children Hospital, 100 Bukit Timah Road, Singapore, 229899.
authors.forename: Nguan Soon
authors.initials: NS
authors.lastname: Tan
authors.affiliation: Department of Telecommunications Electrical Robotics and Biomedical Engineering, Swinburne University of Technology, Hawthorn, Victoria, 3122, Australia.|Melbourne Centre for Nanofabrication, Clayton, 3168, Australia.
authors.forename: Peggy Puk Yik
authors.initials: PP
authors.lastname: Chan
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Singapore. tytan@ntu.edu.sg.
authors.forename: Timothy Thatt Yang
authors.initials: TT
authors.lastname: Tan
authors.affiliation: KK Research Centre, KK Women's and Children Hospital, 100 Bukit Timah Road, Singapore, 229899. cleochoong@ntu.edu.sg.|School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore. cleochoong@ntu.edu.sg.
authors.forename: Cleo
authors.initials: C
authors.lastname: Choong
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D002516:Ceramics; D005399:Fishes; D057809:HEK293 Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D008567:Membranes, Artificial; D010521:Periosteum
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D020100:Hydrogels; D008567:Membranes, Artificial; C020243:calcium phosphate
keywords: biocompatibility; biomaterial; biomineralization; biopolymers; hydrogels