title: Mucin-drugs interaction: The case of theophylline, prednisolone and cephalexin.
abstract: The binding of mucin with three commercially available drugs (theophylline, cephalexin and prednisolone) belonging to different pharmaceutical classes was investigated. The studied drugs are normally used to treat the symptomatology of cystic fibrosis. The interaction between drugs and mucin has been investigated using fluorescence and UV-Vis absorption spectroscopy; quenching mechanism, binding constants, binding sites, thermodynamic parameters and binding distance of the interaction were obtained.
authors.affiliation: Molecular Biotechnology and Health Sciences Department, University of Torino, via Quarello 15, 10135 Torino, Italy.
authors.forename: Carlotta
authors.initials: C
authors.lastname: Pontremoli
authors.affiliation: Department of Chemistry and NIS Interdepartmental Centre, University of Torino, via Pietro Giuria 7, 10125 Torino, Italy.
authors.forename: Nadia
authors.initials: N
authors.lastname: Barbero
authors.affiliation: Department of Chemistry and NIS Interdepartmental Centre, University of Torino, via Pietro Giuria 7, 10125 Torino, Italy.
authors.forename: Guido
authors.initials: G
authors.lastname: Viscardi
authors.affiliation: Molecular Biotechnology and Health Sciences Department, University of Torino, via Quarello 15, 10135 Torino, Italy. Electronic address: sonja.visentin@unito.it.
authors.forename: Sonja
authors.initials: S
authors.lastname: Visentin
mesh_terms: D000818:Animals; D001665:Binding Sites; D002506:Cephalexin / Q000737:chemistry*; D015394:Molecular Structure; D009077:Mucins / Q000737:chemistry*; D011239:Prednisolone / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013270:Stomach / Q000737:chemistry; D013552:Swine; D013806:Theophylline / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D009077:Mucins; D011239:Prednisolone; D013806:Theophylline; D002506:Cephalexin
keywords: Drugs; FRET; Interaction; Mucin; UV–Vis and fluorescence spectroscopy