title: Sphingosine Kinase Regulates Microtubule Dynamics and Organelle Positioning Necessary for Proper G1/S Cell Cycle Transition in Trypanosoma brucei.
abstract: Sphingolipids are important constituents of cell membranes and also serve as mediators of cell signaling and cell recognition. Sphingolipid metabolites such as sphingosine-1-phosphate and ceramide regulate signaling cascades involved in cell proliferation and differentiation, autophagy, inflammation, and apoptosis. Little is known about how sphingolipids and their metabolites function in single-celled eukaryotes. In the present study, we investigated the role of sphingosine kinase (SPHK) in the biology of the protozoan parasite Trypanosoma brucei, the agent of African sleeping sickness. T. brucei SPHK (TbSPHK) is constitutively but differentially expressed during the life cycle of T. brucei. Depletion of TbSPHK in procyclic-form T. brucei causes impaired growth and attenuation in the G1/S phase of the cell cycle. TbSPHK-depleted cells also develop organelle positioning defects and an accumulation of tyrosinated α-tubulin at the elongated posterior end of the cell, known as the "nozzle" phenotype, caused by other molecular perturbations in this organism. Our studies indicate that TbSPHK is involved in G1-to-S cell cycle progression, organelle positioning, and maintenance of cell morphology. Cytotoxicity assays using TbSPHK inhibitors revealed a favorable therapeutic index between T. brucei and human cells, suggesting TbSPHK to be a novel drug target.
  
  
  OBJECTIVE
  Trypanosoma brucei is a single-celled parasite that is transmitted between humans and other animals by the tsetse fly. T. brucei is endemic in sub-Saharan Africa, where over 70 million people and countless livestock are at risk of developing T. brucei infection, called African sleeping sickness, resulting in economic losses of ~$35 million from the loss of cattle alone. New drugs for this infection are sorely needed and scientists are trying to identify essential enzymes in the parasite that can be targets for new therapies. One possible enzyme target is sphingosine kinase, an enzyme involved in the synthesis of lipids important for cell surface integrity and regulation of cell functions. In this study, we found that sphingosine kinase is essential for normal growth and structure of the parasite, raising the possibility that it could be a good target for new chemotherapy for sleeping sickness.
authors.affiliation: Department of Pathology, Northwestern University, Chicago, Illinois, USA Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois, USA Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, Illinois, USA.
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Pasternack
authors.affiliation: Department of Pathology, Northwestern University, Chicago, Illinois, USA Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois, USA Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, Illinois, USA.
authors.forename: Aabha I
authors.initials: AI
authors.lastname: Sharma
authors.affiliation: Department of Pathology, Northwestern University, Chicago, Illinois, USA Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois, USA Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, Illinois, USA.
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Olson
authors.affiliation: Department of Pathology, Northwestern University, Chicago, Illinois, USA Department of Pediatrics, Northwestern University, Chicago, Illinois, USA c-epting@northwestern.edu d-engman@northwestern.edu.
authors.forename: Conrad L
authors.initials: CL
authors.lastname: Epting
authors.affiliation: Department of Pathology, Northwestern University, Chicago, Illinois, USA Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois, USA Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, Illinois, USA c-epting@northwestern.edu d-engman@northwestern.edu.
authors.forename: David M
authors.initials: DM
authors.lastname: Engman
mesh_terms: D000595:Amino Acid Sequence; D001692:Biological Transport; D002453:Cell Cycle; D049109:Cell Proliferation; D008870:Microtubules / Q000378:metabolism*; D008969:Molecular Sequence Data; D001678:Organelle Biogenesis; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D000099157:Sphingosine Kinase
chemical_list: D017853:Phosphotransferases (Alcohol Group Acceptor); D000099157:Sphingosine Kinase