title: In vitro efficacy of Bryophyllum pinnatum leaf extracts as potent therapeutics.
abstract: Leaf extracts of Bryophyllum pinnatum (BPEs) are used in several countries. Contextually, evaluation of the therapeutic potential of these was carried out in this study to explore antioxidant and antityrosinase potential through different in vitro methods. The radical scavenging properties of BPEs were studied using various techniques, based on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) dot blot thin-layer chromatography (TLC) method, electron paramagnetic resonance (EPR) spectroscopy, metal chelation, β-carotene bleaching, inhibition of DNA breakage on agarose gel, and lipid peroxidation inhibition using liver and brain microsomes. EC50 values of the results reflected that aqueous-methanolic BPE was the most active one. Antibrowning potential of the fresh leaf extract showed an antityrosinase property, with EC50 values of enzymatic assay of tyrosinase inhibitory activity further advocating the findings.
authors.affiliation: a Agricultural and Food Engineering Department , Indian Institute of Technology Kharagpur , Kharagpur , India.
authors.forename: Suneel
authors.initials: S
authors.lastname: Gupta
authors.affiliation: a Agricultural and Food Engineering Department , Indian Institute of Technology Kharagpur , Kharagpur , India.
authors.forename: Sunita
authors.initials: S
authors.lastname: Adak
authors.affiliation: b Advanced Technology Development Centre , Indian Institute of Technology Kharagpur , Karagpur , India.
authors.forename: Rajiv Chandra
authors.initials: RC
authors.lastname: Rajak
authors.affiliation: a Agricultural and Food Engineering Department , Indian Institute of Technology Kharagpur , Kharagpur , India.
authors.forename: Rintu
authors.initials: R
authors.lastname: Banerjee
mesh_terms: D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D010936:Plant Extracts
keywords: Antioxidant; B. pinnatum extracts (BPEs); antityrosinase; electron paramagnetic resonance (EPR); lipid peroxidation