title: Exploring water as building bricks in enzyme engineering.
abstract: A novel enzyme engineering strategy for accelerated catalysis based on redesigning a water network through protein backbone deshielding is presented. Fundamental insight into the energetic consequences associated with the design is discussed in the light of experimental results and computer simulations. Using water as biobricks provides unique opportunities when transition state stabilisation is not easily attained by traditional enzyme engineering.
authors.affiliation: KTH Royal Institute of Technology, Division of Industrial Biotechnology, AlbaNova University Centre, 106 91 Stockholm, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Hendil-Forssell
authors.affiliation: KTH Royal Institute of Technology, Division of Industrial Biotechnology, AlbaNova University Centre, 106 91 Stockholm, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Martinelle
authors.affiliation: KTH Royal Institute of Technology, Division of Proteomics & Nanobiotechnology, Science for Life Laboratory, 171 21 Stockholm, Sweden. per-olof.syren@biotech.kth.se.
authors.forename: Per-Olof
authors.initials: PO
authors.lastname: Syrén
mesh_terms: D002384:Catalysis; D004798:Enzymes / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D015202:Protein Engineering; D014867:Water / Q000737:chemistry*
chemical_list: D004798:Enzymes; D014867:Water