title: Application of serex-analysis for identification of human colon cancer antigens.
abstract: BACKGROUND
  Colorectal, lung and breast tumors are the most devastating and frequent malignances in clinical oncology. SEREX-analysis of colon cancer leads to identification of more than hundred antigens which are potential tumor markers. With idea that immunoscreening with pool of allogeneic sera is more productive for antigen isolation, SEREX-analysis was applied to four cases of stages II-IV primary colon tumor and 22 new antigens were isolated.
  
  
  OBJECTIVE
  To characterize 22 primary colon cancer antigens isolated by SEREX-technique.
  
  
  METHODS
  Allogenic screening, real-time PCR analysis.
  
  
  RESULTS
  After allogeneic immunoscreening, for 5 of 22 (22%) isolated antigens were confirmed colon cancer restricted serological profile solely positive for 14% of tested colon cancer sera. Through these five antigens, KY-CC-17/β-actin has cytoskeleton function; KY-CC-14/ACTR1A and KY-CC-19/TSGA2 participate in chromosome segregation; KY-CC-12/FKBP4 regulates steroid receptor function and KY-CC-15/PLRG1 is a component of spliceosome complex. For the last four antigens tested were found aberrant mRNA expression in some cases of colon tumor.
  
  
  CONCLUSIONS
  The exploration of identified antigens may define suitable targets for immunotherapy or diagnostic of colon cancer.
authors.affiliation: Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv 03143, Ukraine.
authors.forename: O M
authors.initials: OM
authors.lastname: Garifulin
authors.affiliation: National Cancer Institute, Kyiv, 03022, Ukraine.
authors.forename: V O
authors.initials: VO
authors.lastname: Kykot
authors.affiliation: Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv 03143, Ukraine.
authors.forename: N Y
authors.initials: NY
authors.lastname: Gridina
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420008, Russian Federation.
authors.forename: R G
authors.initials: RG
authors.lastname: Kiyamova
authors.affiliation: Department of Biochemistry and Molecular Biology, Royal Free and University College Medical School, Cower Street, London WC1E 6BT, United Kingdom.
authors.forename: I T
authors.initials: IT
authors.lastname: Gout
authors.affiliation: Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv 03143, Ukraine.
authors.forename: V V
authors.initials: VV
authors.lastname: Filonenko
mesh_terms: D000328:Adult; D000951:Antigens, Neoplasm / Q000097:blood / Q000235:genetics / Q000276:immunology*; D014408:Biomarkers, Tumor; D003110:Colonic Neoplasms / Q000097:blood / Q000235:genetics / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008297:Male; D008875:Middle Aged
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor