title: Relationships between the use of Embden Meyerhof pathway (EMP) or Phosphoketolase pathway (PKP) and lactate production capabilities of diverse Lactobacillus reuteri strains.
abstract: The aims of this study is to compare the growth and glucose metabolism of three Lactobacillus reuteri strains (i.e. DSM 20016, DSM 17938, and ATCC 53608) which are lactic acid bacteria of interest used for diverse applications such as probiotics implying the production of biomass, or for the production of valuable chemicals (3-hydroxypropionaldehyde, 3-hydroxypropionic acid, 1,3-propanediol). However, the physiological diversity inside the species, even for basic metabolisms, like its capacity of acidification or glucose metabolism, has not been studied yet. In the present work, the growth and metabolism of three strains representative of the species diversity have been studied in batch mode. The strains were compared through characterization of growth kinetics and evaluation of acidification kinetics, substrate consumption and product formation. The results showed significant differences between the three strains which may be explained, at least in part, by variations in the distribution of carbon source between two glycolytic pathways during the bacterial growth: the phosphoketolase or heterolactic pathway (PKP) and the Embden-Meyerhof pathway (EMP). It was also shown that, in the context of obtaining a large amount of biomass, DSM 20016 and DSM 17938 strains were the most effective in terms of growth kinetics. The DSM 17938 strain, which shows the more significant metabolic shift from EMP to PKP when the pH decreases, is more effective for lactate production.
authors.affiliation: Chaire Agro-Biotechnologies Industrielles (ABI) - AgroParisTech, 247 rue Paul Vaillant Couturier, F-51100, Reims, France.|AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.|INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
authors.forename: Grégoire
authors.initials: G
authors.lastname: Burgé
authors.affiliation: AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.|INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Saulou-Bérion
authors.affiliation: AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.|INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
authors.forename: Marwen
authors.initials: M
authors.lastname: Moussa
authors.affiliation: Chaire Agro-Biotechnologies Industrielles (ABI) - AgroParisTech, 247 rue Paul Vaillant Couturier, F-51100, Reims, France.|AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.|INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Allais
authors.affiliation: AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.|INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France.
authors.forename: Violaine
authors.initials: V
authors.lastname: Athes
authors.affiliation: AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France. spinnler@grignon.inra.fr.|INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850, Thiverval-Grignon, France. spinnler@grignon.inra.fr.
authors.forename: Henry-Eric
authors.initials: HE
authors.lastname: Spinnler
mesh_terms: D000446:Aldehyde-Lyases / Q000378:metabolism*; D018533:Biomass; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005285:Fermentation; D016680:Genome, Bacterial; D005947:Glucose / Q000378:metabolism; D005985:Glyceraldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D006019:Glycolysis; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000031:analogs & derivatives / Q000096:biosynthesis*; D052200:Limosilactobacillus reuteri / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D011407:Propane / Q000378:metabolism; D011409:Propylene Glycols / Q000378:metabolism
chemical_list: D003470:Culture Media; D011409:Propylene Glycols; C047158:3-hydroxypropionaldehyde; D019344:Lactic Acid; D005985:Glyceraldehyde; C041787:1,3-propanediol; C031601:hydracrylic acid; D000446:Aldehyde-Lyases; C022367:phosphoketolase; D005947:Glucose; D011407:Propane
keywords: Embden-Meyerhof pathway; Lactobacillus reuteri; Phosphoketolase pathway; acidification kinetics; glucose metabolism; lactate production; microbial growth