title: A major quantitative trait locus conferring subgynoecy in cucumber.
abstract: A major QTL conditioning high degree of femaleness in cucumber was identified by marker analysis and next generation sequencing. Cucumber (Cucumis sativus L.) is a model species for sex determination studies, and its yield is associated with the degree of femaleness. Subgynoecy represents a sex form with a high degree of femaleness for which the genetic basis remains elusive. In this study, genetic analysis in the F2 and BC1 populations developed from a cross between subgynoecious S-2-98 and monoecious M95 suggested a quantitative nature of subgynoecy. Application of simple sequence repeat markers between subgynoecious and monoecious bulks constructed from BC1 plants identified three QTLs: sg3.1, sg6.1, and sg6.2. The major QTL sg3.1 contributed to 54.6% of the phenotypic variation, and its presence was confirmed by genome-wide comparison of SNP profiles between parental lines and a subgynoecious bulk constructed from BC6 plants. Using PCR-based markers developed from the SNP profile, sg3.1 was further delimited to a genomic region of 799 kb. The genetic basis of subgynoecy revealed here shall shed light on the development of elite cultivars with high yield potential.
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Ministry of Agriculture, Sino-Dutch Joint Lab of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Bu
authors.affiliation: Hunan Vegetable Research Institute, Hunan Academy of Agricultural Science, Changsha, 410125, China.
authors.forename: Huiming
authors.initials: H
authors.lastname: Chen
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Ministry of Agriculture, Sino-Dutch Joint Lab of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Ministry of Agriculture, Sino-Dutch Joint Lab of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Ministry of Agriculture, Sino-Dutch Joint Lab of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Dongli
authors.initials: D
authors.lastname: Gao
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Ministry of Agriculture, Sino-Dutch Joint Lab of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Ministry of Agriculture, Sino-Dutch Joint Lab of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. huangsanwen@caas.cn.
authors.forename: Sanwen
authors.initials: S
authors.lastname: Huang
mesh_terms: D002874:Chromosome Mapping; D018553:Cucumis sativus / Q000235:genetics* / Q000502:physiology*; D035264:Flowers / Q000502:physiology; D005819:Genetic Markers; D018745:Genome, Plant; D040582:Inheritance Patterns; D018895:Microsatellite Repeats; D010641:Phenotype; D016133:Polymerase Chain Reaction; D040641:Quantitative Trait Loci; D012098:Reproduction / Q000235:genetics
chemical_list: D005819:Genetic Markers