title: Nuclear Magnetic Resonance (NMR) as a tool for the study of the metabolism of Rickettsia slovaca.
abstract: Rickettsial infections are caused by intracellular bacteria. They do not grow in standard culture media so there are limitations in routine practice to study their metabolism. Nuclear Magnetic Resonance (NMR) spectroscopy is used for identification of metabolites in biological samples. Vero cells infected with Rickettsia slovaca as well as uninfected cells were monitored by (1)H NMR showing the presence of ethanol and lactic acid. As no differences were observed, labeled compounds were added into cultures. When D-[1-13C]glucose was monitored by (13)C NMR no differences among infected and uninfected cells were observed in metabolic profiles. Glucose was transformed into ethanol in all cultures. Monitored experiments carried out with [2-13C]glycine showed differences between infected and uninfected cell cultures spectra. Glycine was partially transformed into serine, but the amount of the serine formed was larger in those infected. Moreover, L-[2-13C]leucine, L-[1-13C]isoleucine and L-[15N]tyrosine were evaluated. No differences among infected and uninfected cells were observed in the metabolic profiles when tyrosine and leucine were monitored. The amino acid L-[1-13C]isoleucine exhibited different metabolism in presence of the R. slovaca, showing a promising behavior as biomarker. In this work we focused on finding one or more compounds that could be metabolized specifically by R. slovaca and could be used as an indicator of its activity.
authors.affiliation: Infectious Diseases Department, Hospital San Pedro-Center for Biomedical Research of La Rioja (CIBIR), Piqueras Street 98, 26006, Logroño, La Rioja, Spain.
authors.forename: Lara
authors.initials: L
authors.lastname: García-Álvarez
authors.affiliation: Departamento de Química, Universidad de La Rioja, Centro de Investigación en Síntesis Química, UA-CSIC, Madre de Dios Street 53, 26006, Logroño, La Rioja, Spain.
authors.forename: Jesús H
authors.initials: JH
authors.lastname: Busto
authors.affiliation: Departamento de Química, Universidad de La Rioja, Centro de Investigación en Síntesis Química, UA-CSIC, Madre de Dios Street 53, 26006, Logroño, La Rioja, Spain.
authors.forename: Jesús M
authors.initials: JM
authors.lastname: Peregrina
authors.affiliation: Infectious Diseases Department, Hospital San Pedro-Center for Biomedical Research of La Rioja (CIBIR), Piqueras Street 98, 26006, Logroño, La Rioja, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Santibáñez
authors.affiliation: Infectious Diseases Department, Hospital San Pedro-Center for Biomedical Research of La Rioja (CIBIR), Piqueras Street 98, 26006, Logroño, La Rioja, Spain.
authors.forename: Aránzazu
authors.initials: A
authors.lastname: Portillo
authors.affiliation: Departamento de Química, Universidad de La Rioja, Centro de Investigación en Síntesis Química, UA-CSIC, Madre de Dios Street 53, 26006, Logroño, La Rioja, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Avenoza
authors.affiliation: Infectious Diseases Department, Hospital San Pedro-Center for Biomedical Research of La Rioja (CIBIR), Piqueras Street 98, 26006, Logroño, La Rioja, Spain. Electronic address: jaoteo@riojsalud.es.
authors.forename: José A
authors.initials: JA
authors.lastname: Oteo
mesh_terms: D000818:Animals; D066241:Carbon-13 Magnetic Resonance Spectroscopy / Q000379:methods*; D002460:Cell Line; D002522:Chlorocebus aethiops; D000431:Ethanol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism*; D007532:Isoleucine / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D012281:Rickettsia / Q000378:metabolism*; D012694:Serine / Q000096:biosynthesis; D014443:Tyrosine / Q000378:metabolism; D014709:Vero Cells
chemical_list: D007532:Isoleucine; D000431:Ethanol; D014443:Tyrosine; D012694:Serine; D007930:Leucine; D005947:Glucose; D005998:Glycine
keywords: Metabolism; NMR; Nuclear Magnetic Resonance; Rickettsia slovaca