title: The Development of a Cat Model of Retinal Detachment and Re-attachment.
abstract: We present an optimized surgical technique for feline retinal detachment which allows for natural re-attachment, reduces retinal scarring and vitreal bands, and allows central placement of the detachment in close proximity to the optic nerve. This enables imaging via Optical Coherence Tomography (OCT) and multifocal electroretinography (mfERG) analysis. Ideal detachment conditions involve a lensectomy followed by a three-port pars plana vitrectomy. A 16-20 % retinal detachment is induced by injecting 8 % C3F8 gas into the subretinal space in the central retina with a 42G cannula. The retinal detachment resolves approximately 6 weeks post-surgery. Imaging is enhanced by using a 7.5 and 20 diopter lens for OCT and mfERG fundus imaging, respectively, to compensate for the removed lens.
authors.affiliation: Ottawa Hospital Research Institute, Regenerative Medicine, K1H 8L6, Ottawa, ON, Canada. swassmer@ohri.ca.|Department of Cellular and Molecular Medicine, University of Ottawa, K1H 8M5, Ottawa, ON, Canada. swassmer@ohri.ca.
authors.forename: Sarah
authors.initials: S
authors.lastname: Wassmer
authors.affiliation: Ottawa Hospital Research Institute, Regenerative Medicine, K1H 8L6, Ottawa, ON, Canada. bleonard@ottawahospital.on.ca.|Department of Ophthalmology, University of Ottawa, K1H 8M5, Ottawa, ON, Canada. bleonard@ottawahospital.on.ca.
authors.forename: Brian C
authors.initials: BC
authors.lastname: Leonard
authors.affiliation: Ottawa Hospital Research Institute, Regenerative Medicine, K1H 8L6, Ottawa, ON, Canada. scoupland@ohri.ca.|Department of Cellular and Molecular Medicine, University of Ottawa, K1H 8M5, Ottawa, ON, Canada. scoupland@ohri.ca.|Department of Ophthalmology, University of Ottawa, K1H 8M5, Ottawa, ON, Canada. scoupland@ohri.ca.
authors.forename: Stuart G
authors.initials: SG
authors.lastname: Coupland
authors.affiliation: Ottawa Hospital Research Institute, Regenerative Medicine, K1H 8L6, Ottawa, ON, Canada. adbaker@ohri.ca.
authors.forename: Adam
authors.initials: A
authors.lastname: Baker
authors.affiliation: Ottawa Hospital Research Institute, Regenerative Medicine, K1H 8L6, Ottawa, ON, Canada. johhamilton@toh.on.ca.
authors.forename: John
authors.initials: J
authors.lastname: Hamilton
authors.affiliation: Ottawa Hospital General Division, Eye Institute, K1H 8L6, Ottawa, ON, Canada. rtorlone@toh.on.ca.
authors.forename: Renée
authors.initials: R
authors.lastname: Torlone
authors.affiliation: Department of Ophthalmology and Visual Sciences, Kellogg Eye Center, University of Michigan Medical School, 48105, Ann Arbor, MI, USA. davzacks@med.umich.edu.
authors.forename: David N
authors.initials: DN
authors.lastname: Zacks
authors.affiliation: Ottawa Hospital Research Institute, Regenerative Medicine, K1H 8L6, Ottawa, ON, Canada. ctsilfidis@ohri.ca.|Department of Cellular and Molecular Medicine, University of Ottawa, K1H 8M5, Ottawa, ON, Canada. ctsilfidis@ohri.ca.|Department of Ophthalmology, University of Ottawa, K1H 8M5, Ottawa, ON, Canada. ctsilfidis@ohri.ca.
authors.forename: Catherine
authors.initials: C
authors.lastname: Tsilfidis
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000175:diagnosis / Q000503:physiopathology / Q000601:surgery*; D002415:Cats; D004596:Electroretinography; D005654:Fundus Oculi; D012160:Retina / Q000473:pathology / Q000601:surgery*; D012163:Retinal Detachment / Q000175:diagnosis / Q000503:physiopathology / Q000601:surgery*; D013997:Time Factors; D041623:Tomography, Optical Coherence; D016896:Treatment Outcome; D014821:Vitrectomy / Q000379:methods*
keywords: C3F8 gas (Octafluoropropane); Feline; Hemorrhage; Lensectomy; Photoreceptor; Retinal detachment; Subretinal space; Vitrectomy