title: Bioactive glass plus laser phototherapy as promise candidates for dentine hypersensitivity treatment.
abstract: Treatments for dentine hypersensitivity (DH) may produce positive effects, though do not have lasting results. We investigated the reparative potential of stem cells derived from deciduous teeth (SHEDs) in response to components delivered from substances used in the treatment of the DH, associated or not to laser phototherapy (LPT), to stimulate dentine formation. SHEDs were submitted to substances delivered from a laboratorial P-rich bioactive glass [57SiO2-26CaO-17P2O5(wt %)] or a commercially available desensitizer (Gluma® Desensitizer), associated (or not) to LPT (InGAlP diode laser, 660 nm, 0.028 cm2, 20 mW, 5 J/cm2, 7 s, contact mode). Biomaterial characterization was performed by X-ray diffraction, scanning electron microscopy and the particle size was evaluated by dynamic light scattering. SHEDs proliferation and differentiation were analyzed by MTT and Alizarin Red staining, respectively. The conditioned media used in these tests were evaluated regarding their pH and the ionic concentration changes due to ions leached from the bioactive glass (BG). BG majority presented a non-crystalline solid structure and mixed particle sizes characterized by the agglomeration of nanoparticles. Cultures treated with BG alone or in association to LPT showed improved cell growth in relation to Gluma®(p < 0.05). Gluma®was cytotoxic in all tested conditions, regardless irradiated or not. BG associated to LPT induced intense mineral matrix formation. In conclusion, BG releases ionic dissolution products able to promote SHEDs differentiation. BG associated to LPT improves SHEDs proliferation and differentiation in vitro, and may be a promise therapeutic approach for the DH treatment. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 107-116, 2017.
authors.affiliation: Restorative Dentistry Department, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Talita Christine Camilo
authors.initials: TC
authors.lastname: Lopez
authors.affiliation: Restorative Dentistry Department, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Ivana Márcia Alves
authors.initials: IM
authors.lastname: Diniz
authors.affiliation: Restorative Dentistry Department, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Leila Soares
authors.initials: LS
authors.lastname: Ferreira
authors.affiliation: Federal University of ABC, Human and Natural Sciences Center, Sao Paulo, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Marchi
authors.affiliation: Federal University of ABC, Human and Natural Sciences Center, Sao Paulo, Brazil.
authors.forename: Roger
authors.initials: R
authors.lastname: Borges
authors.affiliation: Restorative Dentistry Department, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Sueli Patricia Harumi Miyagi
authors.initials: SP
authors.lastname: de Cara
authors.affiliation: Restorative Dentistry Department, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil.|Restorative Dentistry Department, School of Dentistry, Federal University of Pará, Belém, Pará, Brazil.
authors.forename: Roberta
authors.initials: R
authors.lastname: D'Almeida-Couto
authors.affiliation: Restorative Dentistry Department, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Márcia Martins
authors.initials: MM
authors.lastname: Marques
mesh_terms: D002516:Ceramics / Q000737:chemistry / Q000494:pharmacology; D003807:Dentin Sensitivity / Q000628:therapy*; D005260:Female; D006801:Humans; D028022:Low-Level Light Therapy; D008297:Male; D008422:Materials Testing; D010316:Particle Size; D013234:Stem Cells / Q000378:metabolism*; D014094:Tooth, Deciduous / Q000378:metabolism*
chemical_list: C022107:Bioglass
keywords: bioactive glass; dentine hypersensitivity; laser phototherapy; mesenchymal stem cells