title: A Fluorescence Polarization Assay To Detect Steroid Hormone Traces in Milk.
abstract: Steroids are a class of hormones improperly used in livestock as growth-promoting agents. Due to their high risk for human health, the European Union (EU) has strictly forbidden the administration of all natural and synthetic steroid hormones to food-producing animals, and the development of new rapid detection methods are greatly encouraged. This work reports a novel fluorescence polarization assay, ready to use, capable of detecting 17β-estradiol directly in milk samples with a low limit of detection of <10 pmol. It is based on the coupling of monospecific antibodies against 17β-estradiol and fluorophores, capable of modulating the fluorescence polarization emission on the basis of the specific binding of antibodies to fluorescence-labeled 17β-estradiol derivative. The successful detection of 17β-estradiol has disclosed the development of an efficient method, easily extensible to any food matrix and having the potential to become a milestone in food quality and safety.
authors.affiliation: Institute of Food Science, CNR , Via Roma 52, 83100 Avellino, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Varriale
authors.affiliation: Institute of Food Science, CNR , Via Roma 52, 83100 Avellino, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Pennacchio
authors.affiliation: Institute of Food Science, CNR , Via Roma 52, 83100 Avellino, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Pinto
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Napoli "Federico II" , Via D. Montesano 49, 80131 Napoli, Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Oliviero
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Napoli "Federico II" , Via D. Montesano 49, 80131 Napoli, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: D'Errico
authors.affiliation: Institute of Food Science, CNR , Via Roma 52, 83100 Avellino, Italy.
authors.forename: Adelia
authors.initials: A
authors.lastname: Majoli
authors.affiliation: Institute of Food Science, CNR , Via Roma 52, 83100 Avellino, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Scala
authors.affiliation: Institute of Food Science, CNR , Via Roma 52, 83100 Avellino, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Capo
authors.affiliation: Institute of Food Science, CNR , Via Roma 52, 83100 Avellino, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Pennacchio
authors.affiliation: Institute of Food Science, CNR , Via Roma 52, 83100 Avellino, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Di Giovanni
authors.affiliation: Institute of Food Science, CNR , Via Roma 52, 83100 Avellino, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Staiano
authors.affiliation: Institute of Food Science, CNR , Via Roma 52, 83100 Avellino, Italy.
authors.forename: Sabato
authors.initials: S
authors.lastname: D'Auria
mesh_terms: D000818:Animals; D005454:Fluorescence Polarization / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D006728:Hormones / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D013256:Steroids / Q000032:analysis*
chemical_list: D006728:Hormones; D013256:Steroids
keywords: antibody; biosensors; estradiol; fluorescence; milk