title: Designing of Biodegradable and Biocompatible Release and Delivery Systems of Selected Antioxidants Used in Cosmetology.
abstract: Conjugates of antioxidants p-anisic (p-AA) and vanillic (VA) acids with nontoxic, biocompatible, and biodegradedable oligo-(R,S)-(3-hydoxybutyrate) carrier were synthesized, and their structural and biological characterization was performed. The molecular structure of the bioconjugates, in which antioxidants are covalently bonded with oligo(3-hydroxybutyrate) (OHB) chains, has been proven by mass spectrometry supported by NMR. The bioconjugate hydrolytic degradation studies allowed gaining thorough insight into the hydrolysis process and confirmed the release of p-AA and VA. In vitro studies demonstrated that all of the conjugates studied were well tolerated by KB and HaCaT cell lines, as they had no marked cytotoxicity, while conjugates with a relatively short OHB carrier are optimal to support keratinocyte function. The preliminary study of the biological activity confirmed the protective effect of VA-OHB conjugates against H2O2-induced lipid peroxidation in human keratinocytes (HaCaT). It was also demonstrated that the selected bioconjugates can penetrate all layers of the skin, which shows their functionality and opens up their potential application in cosmetology.
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , 34 M. Curie-Sklodowskiej Street, 41-819 Zabrze, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Maksymiak
authors.affiliation: Dr Irena Eris Centre for Science and Research , 107A Pulawska Street, 02-595 Warszawa, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Debowska
authors.affiliation: Dr Irena Eris Centre for Science and Research , 107A Pulawska Street, 02-595 Warszawa, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Bazela
authors.affiliation: Dr Irena Eris Centre for Science and Research , 107A Pulawska Street, 02-595 Warszawa, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Dzwigalowska
authors.affiliation: School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland, Chair and Department of Biopharmacy, 8 Jednosci Street, 41-208 Sosnowiec, Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Orchel
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , 34 M. Curie-Sklodowskiej Street, 41-819 Zabrze, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Jelonek
authors.affiliation: Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University , 72 Powstancow Wielkopolskich Street, 70-111 Szczecin, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Dolegowska
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , 34 M. Curie-Sklodowskiej Street, 41-819 Zabrze, Poland.|School of Biology, Chemistry and Forensic Science, Faculty of Science and Engineering, University of Wolverhampton , Wulfruna Street, Wolverhampton, WV1 1SB, United Kingdom.
authors.forename: Marek
authors.initials: M
authors.lastname: Kowalczuk
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences , 34 M. Curie-Sklodowskiej Street, 41-819 Zabrze, Poland.
authors.forename: Grazyna
authors.initials: G
authors.lastname: Adamus
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D003358:Cosmetics / Q000737:chemistry*; D016503:Drug Delivery Systems; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D006868:Hydrolysis; D062425:Hydroxybenzoate Ethers / Q000737:chemistry / Q000494:pharmacology; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D015394:Molecular Structure; D012867:Skin / Q000166:cytology / Q000378:metabolism; D014641:Vanillic Acid / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D003358:Cosmetics; D062425:Hydroxybenzoate Ethers; C004520:4-anisic acid; D006861:Hydrogen Peroxide; D014641:Vanillic Acid; D020155:3-Hydroxybutyric Acid