title: Pharyngeal pumping inhibition and avoidance by acute exposure to high CO2 levels are both regulated by the BAG neurons via different molecular pathways.
abstract: Carbon dioxide (CO2) is a key molecule in many biological processes. Studies in humans, mice, D. melanogaster, C. elegans, unicellular organisms and plants have shed light on the molecular pathways activated by elevated levels of CO2. However, the mechanisms that organisms use to sense and respond to high CO2 levels remain largely unknown. Previous work has shown that C. elegans quickly avoid elevated CO2 levels using mechanisms that involve the BAG, ASE and AFD neurons via cGMP- and calcium- signaling pathways. Here, we discuss our recent finding that exposure of C. elegans to high CO2 levels leads to a very rapid cessation in the contraction of the pharynx muscles. Surprisingly, none of the tested CO2 avoidance mutants affected the rapid pumping inhibition response to elevated CO2 levels. A forward genetic screen identified that the hid-1-mediated pathway of dense core vesicle maturation regulates the pumping inhibition, probably through affecting neuropeptide secretion. Genetic studies and laser ablation experiments showed that the CO2 response of the pharyngeal muscle pumping is regulated by the BAG neurons, the same neurons that mediate CO2 avoidance.
authors.affiliation: Department of Genetics; Institute of Life Sciences ; Hebrew University of Jerusalem ; Jerusalem, Israel.
authors.forename: Kfir
authors.initials: K
authors.lastname: Sharabi
authors.affiliation: Department of Genetics; Institute of Life Sciences ; Hebrew University of Jerusalem ; Jerusalem, Israel.
authors.forename: Chayki
authors.initials: C
authors.lastname: Charar
authors.affiliation: Department of Genetics; Institute of Life Sciences ; Hebrew University of Jerusalem ; Jerusalem, Israel.
authors.forename: Yosef
authors.initials: Y
authors.lastname: Gruenbaum
keywords: BAG neurons; CO2 avoidance; Caenorhabditis elegans; dense core vesicles; pharynx