title: Organized Aggregation of Porphyrins in Lipid Bilayers for Third Harmonic Generation Microscopy.
abstract: Nonlinear optical microscopy has become a powerful tool for high-resolution imaging of cellular and subcellular composition, morphology, and interactions because of its high spatial resolution, deep penetration, and low photo-damage to tissue. Developing specific harmonic probes is essential for exploiting nonlinear microscopic imaging for biomedical applications. We report an organized aggregate of porphyrins (OAP) that formed within lipidic nanoparticles showing fingerprint spectroscopic properties, structure-associated second harmonic generation, and superradiant third harmonic generation. The OAP facilitated harmonic microscopic imaging of living cells with significantly enhanced contrast. The structure-dependent switch between harmonic (OAP-intact) and fluorescence (OAP-disrupted) generation enabled real-time multi-modality imaging of the cellular fate of nanoparticles. Robustly produced under various conditions and easily incorporated into pre-formed lipid nanovesicles, OAP provides a biocompatible nanoplatform for harmonic imaging.
authors.affiliation: Princess Margaret Cancer Center and Techna Institute, UHN (Canada) http://www.utoronto.ca/zhenglab.|Medical Isotopes Research Center, Peking University (China).|Department of Medical Biophysics, University of Toronto (Canada).
authors.forename: Liyang
authors.initials: L
authors.lastname: Cui
authors.affiliation: Department of Chemical and Physical Sciences and Department of Physics, University of Toronto (Canada).
authors.forename: Danielle
authors.initials: D
authors.lastname: Tokarz
authors.affiliation: Department of Chemical and Physical Sciences and Department of Physics, University of Toronto (Canada).
authors.forename: Richard
authors.initials: R
authors.lastname: Cisek
authors.affiliation: Princess Margaret Cancer Center and Techna Institute, UHN (Canada) http://www.utoronto.ca/zhenglab.
authors.forename: Kenneth K
authors.initials: KK
authors.lastname: Ng
authors.affiliation: Medical Isotopes Research Center, Peking University (China).
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.affiliation: Princess Margaret Cancer Center and Techna Institute, UHN (Canada) http://www.utoronto.ca/zhenglab.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Chemical and Physical Sciences and Department of Physics, University of Toronto (Canada).
authors.forename: Virginijus
authors.initials: V
authors.lastname: Barzda
authors.affiliation: Princess Margaret Cancer Center and Techna Institute, UHN (Canada) http://www.utoronto.ca/zhenglab. gang.zheng@uhnres.utoronto.ca.|Department of Medical Biophysics, University of Toronto (Canada). gang.zheng@uhnres.utoronto.ca.
authors.forename: Gang
authors.initials: G
authors.lastname: Zheng
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D008853:Microscopy / Q000379:methods*; D011166:Porphyrins / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D011166:Porphyrins
keywords: harmonic generation; multimodality imaging; nonlinear optical properties; organized aggregation; porphyrin nanoparticles