title: Live-Cell Imaging of Phagosome Motility in Primary Mouse RPE Cells.
abstract: The retinal pigment epithelium (RPE) is a post-mitotic epithelial monolayer situated between the light-sensitive photoreceptors and the choriocapillaris. Given its vital functions for healthy vision, the RPE is a primary target for insults that result in blinding diseases, including age-related macular degeneration (AMD). One such function is the phagocytosis and digestion of shed photoreceptor outer segments. In the present study, we examined the process of trafficking of outer segment disk membranes in live cultures of primary mouse RPE, using high speed spinning disk confocal microscopy. This approach has enabled us to track phagosomes, and determine parameters of their motility, which are important for their efficient degradation.
authors.affiliation: UCLA School of Medicine, Jules Stein Eye Institute, 100 Stein Plaza, 90095, Los Angeles, CA, USA.
authors.forename: Roni
authors.initials: R
authors.lastname: Hazim
authors.affiliation: UCLA School of Medicine, Jules Stein Eye Institute, 100 Stein Plaza, 90095, Los Angeles, CA, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Jiang
authors.affiliation: UCLA School of Medicine, Jules Stein Eye Institute, 100 Stein Plaza, 90095, Los Angeles, CA, USA.
authors.forename: Julian
authors.initials: J
authors.lastname: Esteve-Rudd
authors.affiliation: UCLA School of Medicine, Jules Stein Eye Institute, 100 Stein Plaza, 90095, Los Angeles, CA, USA.
authors.forename: Tanja
authors.initials: T
authors.lastname: Diemer
authors.affiliation: UCLA School of Medicine, Jules Stein Eye Institute, 100 Stein Plaza, 90095, Los Angeles, CA, USA.
authors.forename: Vanda S
authors.initials: VS
authors.lastname: Lopes
authors.affiliation: UCLA School of Medicine, Jules Stein Eye Institute, 100 Stein Plaza, 90095, Los Angeles, CA, USA. dswilliams@ucla.edu.
authors.forename: David S
authors.initials: DS
authors.lastname: Williams
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D007700:Kinetics; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D010587:Phagocytosis; D010588:Phagosomes / Q000378:metabolism*; D061251:Primary Cell Culture; D055214:Retinal Photoreceptor Cell Outer Segment / Q000378:metabolism; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000378:metabolism*; D059008:Time-Lapse Imaging / Q000379:methods*
keywords: Intracellular trafficking; Live-cell imaging; Phagocytosis; Photoreceptor outer segment; Retinal pigment epithelium