title: Reactive Charged Droplets for Reduction of Matrix Effects in Electrospray Ionization Mass Spectrometry.
abstract: A new quantitative contained-electrospray (ES) process is described here that employs a movable ES emitter to control the reactivity of charged microdroplets by varying their exposure time with acid vapor. The method allows elimination of ion suppression effects caused by the presence of various surface active compounds that coelute with the analyte. For mixtures, contained-ESI mass spectrometric analysis produces relative ion intensities that reflect the true concentrations of analytes in solution. The mechanism for this effect has been elucidated and ascribed to the generation of fine initial droplets in the presence of a high abundance of protons; together, these two factors eliminate competition for charge and space during ion formation. Examples of analytes tested include steroids, phospholipids, phosphopeptides, and sialylated glycans. At least 1 order of magnitude improvement in detection limits, sensitivity, and accuracy of detection was observed when compared to conventional electrospray.
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University , 100 West 18th Ave, Columbus, Ohio 43210, United States.
authors.forename: Dmytro S
authors.initials: DS
authors.lastname: Kulyk
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University , 100 West 18th Ave, Columbus, Ohio 43210, United States.
authors.forename: Colbert F
authors.initials: CF
authors.lastname: Miller
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University , 100 West 18th Ave, Columbus, Ohio 43210, United States.
authors.forename: Abraham K
authors.initials: AK
authors.lastname: Badu-Tawiah
mesh_terms: D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013499:Surface Properties
chemical_list: D012996:Solutions