title: Analgesic-antitumor peptide inhibits the migration and invasion of HepG2 cells by an upregulated VGSC β1 subunit.
abstract: Analgesic-antitumor peptide (AGAP), one of the scorpion toxin polypeptides, has been shown to have an antitumor activity. Recombinant AGAP (rAGAP) was shown to affect the migration and invasion of HepG2 cells via a voltage-gated sodium channel (VGSC) β1 subunit. The VGSC β1 subunit was validated as a cell adhesion molecule (CAM) in human hepatocellular carcinoma (HCC) cell lines. rAGAP suppresses the migration and invasion of HepG2 cells but has no significant effect of human liver HL7702 cells without β1 subunit expression. rAGAP inhibits the migration and invasion of the cells when the VGSC β1 subunit is overexpressed in HL7702 cells. To explain these findings, VGSC β1 subunit messenger RNA (mRNA) and protein levels were measured. The β1 subunit protein level was upregulated in a dose-dependent manner following treatment with rAGAP while there was no significant change in the mRNA level, so rAGAP might be an active component of the VGSC β1 subunit.
authors.affiliation: School of Life Science, Shenyang Pharmaceutical University, P.O. Box 17, 103 WenHua Road, Shenyang, 110016, People's Republic of China.
authors.forename: Guili
authors.initials: G
authors.lastname: Guo
authors.affiliation: School of Medical Devices, Shenyang Pharmaceutical University, Benxi, 117004, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cui
authors.affiliation: School of Life Science, Shenyang Pharmaceutical University, P.O. Box 17, 103 WenHua Road, Shenyang, 110016, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Life Science, Shenyang Pharmaceutical University, P.O. Box 17, 103 WenHua Road, Shenyang, 110016, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Life Science, Shenyang Pharmaceutical University, P.O. Box 17, 103 WenHua Road, Shenyang, 110016, People's Republic of China.
authors.forename: Mingyi
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Anshan Central Hospital, Ansha, 114001, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.affiliation: School of Medical Devices, Shenyang Pharmaceutical University, Benxi, 117004, People's Republic of China. Jinghaizhang82@gmail.com.|School of Medical Devices, Shenyang Pharmaceutical University, 103 WenHua Road, Shenyang, 110016, People's Republic of China. Jinghaizhang82@gmail.com.
authors.forename: Jinghai
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Life Science, Shenyang Pharmaceutical University, P.O. Box 17, 103 WenHua Road, Shenyang, 110016, People's Republic of China. liuyanfeng@syphu.edu.cn.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D009361:Neoplasm Invasiveness; D010455:Peptides / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D012604:Scorpion Venoms / Q000494:pharmacology*; D062547:Voltage-Gated Sodium Channel beta-1 Subunit / Q000032:analysis / Q000502:physiology*
chemical_list: D000970:Antineoplastic Agents; D010455:Peptides; D011994:Recombinant Proteins; D012604:Scorpion Venoms; D062547:Voltage-Gated Sodium Channel beta-1 Subunit
keywords: Analgesic-antitumor peptide; HepG2; Invasion; Migration; Voltage-gated sodium channel β1 subunit