title: Molecular Mechanisms for High Hydrostatic Pressure-Induced Wing Mutagenesis in Drosophila melanogaster.
abstract: Although High hydrostatic pressure (HHP) as an important physical and chemical tool has been increasingly applied to research of organism, the response mechanisms of organism to HHP have not been elucidated clearly thus far. To identify mutagenic mechanisms of HHP on organisms, here, we treated Drosophila melanogaster (D. melanogaster) eggs with HHP. Approximately 75% of the surviving flies showed significant morphological abnormalities from the egg to the adult stages compared with control flies (p < 0.05). Some eggs displayed abnormal chorionic appendages, some larvae were large and red, and some adult flies showed wing abnormalities. Abnormal wing phenotypes of D. melanogaster induced by HHP were used to investigate the mutagenic mechanisms of HHP on organism. Thus 285 differentially expressed genes associated with wing mutations were identified using Affymetrix Drosophila Genome Array 2.0 and verified with RT-PCR. We also compared wing development-related central genes in the mutant flies with control flies using DNA sequencing to show two point mutations in the vestigial (vg) gene. This study revealed the mutagenic mechanisms of HHP-induced mutagenesis in D. melanogaster and provided a new model for the study of evolution on organisms.
authors.affiliation: State Key Laboratory of Superhard Materials, Jilin University, Changchun, 130012, P. R. China.|College of Life Science, Jilin University, Changchun, 130012, P. R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Superhard Materials, Jilin University, Changchun, 130012, P. R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Superhard Materials, Jilin University, Changchun, 130012, P. R. China.
authors.forename: Guanjun
authors.initials: G
authors.lastname: Xiao
authors.affiliation: College of Life Science, Jilin University, Changchun, 130012, P. R. China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Ma
authors.affiliation: College of Life Science, Jilin University, Changchun, 130012, P. R. China.
authors.forename: Bingying
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Life Science, Jilin University, Changchun, 130012, P. R. China.
authors.forename: Sile
authors.initials: S
authors.lastname: Shen
authors.affiliation: College of Life Science, Jilin University, Changchun, 130012, P. R. China.
authors.forename: Xueqi
authors.initials: X
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Superhard Materials, Jilin University, Changchun, 130012, P. R. China.
authors.forename: Guangtian
authors.initials: G
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Superhard Materials, Jilin University, Changchun, 130012, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zou
mesh_terms: D000818:Animals; D001483:Base Sequence; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D063990:Gene Ontology; D005838:Genotype; D006874:Hydrostatic Pressure; D007814:Larva / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D017354:Point Mutation; D014921:Wings, Animal / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*; D015053:Zygote / Q000254:growth & development
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; C095064:vg protein, Drosophila