title: Heat-shock pretreatment reduces expression and release of TSLP from keratinocytes under Th2 environment.
abstract: BACKGROUND
  Atopic dermatitis is a chronic, relapsing inflammatory disease of the skin. Current therapy is not curative, and recalcitrant disease is a big stress and challenge for parents and physicians. This study explored the potential role of heat-shock protein 70 (HSP-70) and its anti-inflammatory effects on keratinocyte under TH2 environment.
  
  
  METHODS
  Human keratinocyte cell line (HaCa T) was stimulated with IL-4, IL-13, and TNF-α to synthesize and secrete thymic stromal lymphopoietin (TSLP), an important cytokine of immunopathogenesis in atopic dermatitis. Heat shock was performed by immersing the cell-contained flash into a water bath of 45°C for 20 min. Cell viability, TSLP expression, and secretion of HaCa T cells were measured and compared. Possible regulatory mechanisms influencing the expression of TSLP, such as the STAT6 and NF-κB signal pathways, were investigated.
  
  
  RESULTS
  Heat-shock treatment induced intracellular HSP-70 expression in HaCa T cells without affecting cell viability. The induced expression and secretion of TSLP in HaCa T cells were suppressed by heat shock. The NF-κB signal pathway was inhibited by heat shock, leading to decreased TSLP expression and secretion.
  
  
  CONCLUSIONS
  Heat stress-induced HSPs can significantly reduce the production and secretion of TSLP from HaCaT cells under Th2 environment. Thus, the evidence highlights the potential role of HSP-70 for atopic dermatitis in the future.
authors.affiliation: Frontier Molecular Medical Research Center in Children, Changhua Christian Children Hospital, Changhua County, Taiwan.|Institute of Biomedical Sciences, National Chung Hsing University, Taichung City, Taiwan.
authors.forename: Jun-Kai
authors.initials: JK
authors.lastname: Kao
authors.affiliation: Frontier Molecular Medical Research Center in Children, Changhua Christian Children Hospital, Changhua County, Taiwan.
authors.forename: Cheng-Han
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Frontier Molecular Medical Research Center in Children, Changhua Christian Children Hospital, Changhua County, Taiwan.
authors.forename: Ming-Sheng
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Frontier Molecular Medical Research Center in Children, Changhua Christian Children Hospital, Changhua County, Taiwan.
authors.forename: Cheng-Sheng
authors.initials: CS
authors.lastname: Hsu
authors.affiliation: Frontier Molecular Medical Research Center in Children, Changhua Christian Children Hospital, Changhua County, Taiwan.
authors.forename: Long-Yen
authors.initials: LY
authors.lastname: Tsao
authors.affiliation: Department of Pediatrics, Changhua Christian Children Hospital, Changhua County, Taiwan.|School of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan.|School of Medicine, Chung Shan Medical University, Taichung City, Taiwan.
authors.forename: Yi-Giien
authors.initials: YG
authors.lastname: Tsai
authors.affiliation: Institute of Biomedical Sciences, National Chung Hsing University, Taichung City, Taiwan.|Department of Education and Research, Taichung Veterans General Hospital, Taichung City, Taiwan.|Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung City, Taiwan.
authors.forename: Jeng-Jer
authors.initials: JJ
authors.lastname: Shieh
authors.affiliation: Frontier Molecular Medical Research Center in Children, Changhua Christian Children Hospital, Changhua County, Taiwan.|Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung City, Taiwan.
authors.forename: Rei-Cheng
authors.initials: RC
authors.lastname: Yang
mesh_terms: D002460:Cell Line; D060833:Cellular Microenvironment; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003876:Dermatitis, Atopic / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000517:prevention & control; D015536:Down-Regulation; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D018869:Heat-Shock Response; D006801:Humans; D018836:Inflammation Mediators / Q000276:immunology / Q000378:metabolism*; D015603:Keratinocytes / Q000276:immunology / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors; D014158:Transcription, Genetic; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D018840:HSP70 Heat-Shock Proteins; D018836:Inflammation Mediators; D016328:NF-kappa B; D000094632:Thymic Stromal Lymphopoietin
keywords: atopic dermatitis; heat shock; heat-shock protein; thymic stromal lymphopoietin