title: Roseovarius algicolus sp. nov., isolated from culture fluid of Cochlidinium polykrikoides.
abstract: A Gram-negative, aerobic, non-motile, catalase and oxidase positive rod-shaped bacterium, designated strain 4CoA2(T), was isolated from Cochlidinium polykrikoides culture fluid in the laboratory. The strain formed circular, glistening, grayish yellow colonies and grew at pH 7-8 and 28 °C optimally. On the basis of 16S rRNA gene sequence similarity, the strain was shown to be phylogenetically related to Roseovarius indicus B108(T) (97.6 %), Roseovarius halotolerans HJ50(T) (97.3 %), Roseovarius pacificus 2-81(T) (96.6 %), Roseovarius litoreus GSW-M15(T) (96.3 %) and Roseovarius tolerans EL-172(T) (94.27 %). Chemotaxonomic data revealed that the strain 4CoA2(T) possesses ubiquinone Q-10, and the predominant fatty acid is C18:1 ω6c or C18:1 ω7c. The polar lipids profile of the strain 4CoA2(T) included major amounts of phosphatidylglycerol, phosphatidylcholine, and diphosphatidyl glycerol. The DNA G + C content of the strain 4CoA2(T) is 62.0 %. Strain 4CoA2(T) exhibited relatively low level of DNA hybridization values (<35 %) with other Roseovarius species. On the basis of its phenotypic, genotypic properties and its phylogenetic data, strain 4CoA2(T) can be concluded to represent a novel species in the genus Roseovarius, for which the name Roseovarius algicolus sp. nov. is proposed. The type strain is 4CoA2(T) (=JCM 30823(T), =KEMB 3501-06(T)).
authors.affiliation: Department of Bio Technology, Division of Environmental & Life Science, College of Natural Science, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon-si, 443-760, Republic of Korea.
authors.forename: Saet-Byeol
authors.initials: SB
authors.lastname: Moon
authors.affiliation: Department of Life Science, School of Natural Science, Kyonggi University, San 94-6, Iui-dong, Yeongtong-gu, Suwon-si, 443-760, Republic of Korea. sslee@kyonggi.ac.kr.
authors.forename: Sang-Seob
authors.initials: SS
authors.lastname: Lee
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000032:analysis; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004141:Dinoflagellida / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; D002374:Catalase
keywords: Rhodobacteraceae; Roseovarius algicolus; Taxonomy