title: Protective role of caffeic acid in an Aβ25-35-induced Alzheimer's disease model.
abstract: OBJECTIVE
  Alzheimer's disease (AD) is characterized by deficits in memory and cognitive functions. The accumulation of amyloid beta peptide (Aβ) and oxidative stress in the brain are the most common causes of AD.
  
  
  METHODS
  Caffeic acid (CA) is an active phenolic compound that has a variety of pharmacological actions. We studied the protective abilities of CA in an Aβ25-35-injected AD mouse model. CA was administered at an oral dose of 10 or 50 mg/kg/day for 2 weeks. Behavioral tests including T-maze, object recognition, and Morris water maze were carried out to assess cognitive abilities. In addition, lipid peroxidation and nitric oxide (NO) production in the brain were measured to investigate the protective effect of CA in oxidative stress.
  
  
  RESULTS
  In the T-maze and object recognition tests, novel route awareness and novel object recognition were improved by oral administration of CA compared with the Aβ25-35-injected control group. These results indicate that administration of CA improved spatial cognitive and memory functions. The Morris water maze test showed that memory function was enhanced by administration of CA. In addition, CA inhibited lipid peroxidation and NO formation in the liver, kidney, and brain compared with the Aβ25-35-injected control group. In particular, CA 50 mg/kg/day showed the stronger protective effect from cognitive impairment than CA 10 mg/kg/day.
  
  
  CONCLUSIONS
  The present results suggest that CA improves Aβ25-35-induced memory deficits and cognitive impairment through inhibition of lipid peroxidation and NO production.
authors.affiliation: Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 609-735, Korea.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 609-735, Korea.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 609-735, Korea.
authors.forename: Ji Myung
authors.initials: JM
authors.lastname: Choi
authors.affiliation: Department of Integrative Plant Science, Chung-Ang University, Seodong-daero 4726, Daedeok-myeon, Anseong 456-756, Korea.
authors.forename: Sanghyun
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 609-735, Korea.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Cho
keywords: Alzheimer's disease; Caffeic acid; amyloid beta; nitric oxide; oxidative stress