title: Combination of MiR-378 and MiR-210 Serum Levels Enables Sensitive Detection of Renal Cell Carcinoma.
abstract: Serum microRNAs are emerging as a clinically useful tool for early and non-invasive detection of various cancer types including renal cell carcinoma (RCC). Based on our previous results, we performed the study to analyze circulating serum miR-378 and miR-210 in patients with various histological subtypes of RCC. RNA was purified from blood serum samples of 195 RCC patients and 100 healthy controls. The levels of miR-378 and miR-210 in serum were determined absolutely using quantitative real-time PCR. Pre- and postoperative levels of both microRNAs were compared in 20 RCC patients. Significantly increased serum levels of both miR-378 and miR-210 enabled to clearly distinguish RCC patients and healthy controls with 80% sensitivity and 78% specificity if analyzed in combination (p<0.0001), and their levels significantly decreased in the time period of three months after radical nephrectomy (p<0.0001). Increased level of miR-378 positively correlates with disease-free survival (p=0.036) and clinical stage (p=0.0476). The analysis of serum miR-378 and miR-210 proved their potential to serve as powerful non-invasive diagnostic and prognostic biomarkers in RCC.
authors.affiliation: Department of Urology, University Hospital Brno and Masaryk University Brno, Brno 62500, Czech Republic. michal.fedorko@fnbrno.cz.
authors.forename: Michal
authors.initials: M
authors.lastname: Fedorko
authors.affiliation: Department of Urologic Oncology, Masaryk Memorial Cancer Institute, Brno 65653, Czech Republic. stanik@mou.cz.
authors.forename: Michal
authors.initials: M
authors.lastname: Stanik
authors.affiliation: Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno 65653, Czech Republic. 269069@mail.muni.cz.|Central European Institute of Technology, Masaryk University, Brno 62500, Czech Republic. 269069@mail.muni.cz.
authors.forename: Robert
authors.initials: R
authors.lastname: Iliev
authors.affiliation: Central European Institute of Technology, Masaryk University, Brno 62500, Czech Republic. martina.redova@gmail.com.
authors.forename: Martina
authors.initials: M
authors.lastname: Redova-Lojova
authors.affiliation: Central European Institute of Technology, Masaryk University, Brno 62500, Czech Republic. tana.machackova@gmail.com.
authors.forename: Tana
authors.initials: T
authors.lastname: Machackova
authors.affiliation: Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno 65653, Czech Republic. msvoboda@mou.cz.
authors.forename: Marek
authors.initials: M
authors.lastname: Svoboda
authors.affiliation: Department of Urology, University Hospital Brno and Masaryk University Brno, Brno 62500, Czech Republic. dalibor.pacik@gmail.com.
authors.forename: Dalibor
authors.initials: D
authors.lastname: Pacik
authors.affiliation: Department of Urologic Oncology, Masaryk Memorial Cancer Institute, Brno 65653, Czech Republic. dolezel@mou.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Dolezel
authors.affiliation: Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno 65653, Czech Republic. slaby@mou.cz.|Central European Institute of Technology, Masaryk University, Brno 62500, Czech Republic. slaby@mou.cz.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Slaby
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D002292:Carcinoma, Renal Cell / Q000097:blood* / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000097:blood* / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged
chemical_list: D014408:Biomarkers, Tumor; C543800:MIRN210 microRNA, human; C526012:MIRN378 microRNA, human; D035683:MicroRNAs
keywords: biomarker; blood serum; microRNA; renal cell carcinoma