title: N-(2-hydroxypropyl) methacrylamide based cryogels--synthesis and biomimetic modification for stem cell applications.
abstract: The design of favorable mechanical properties and suitable surface modifications of hydrogels in order to stimulate specific cell response is a great challenge. N-(2-Hydroxypropyl) methacryl-amide (HPMA) was utilized to form macroporous cryogel scaffolds for stem cell applications. Furthermore, one group of scaffolds was enhanced by copolymerization of HPMA with methacryloyl-GGGRGDS-OH peptide in an effort to integrate biomimetic adhesion sites. The cryogels were characterized by stiffness and equilibrium swelling measurements as well as by scanning electron microscopy. Cell culture experiments were performed with human adipose-derived stem cells and substrates were found completely non-toxic. Moreover, RGDS-enriched cryogels supported cell attachment, spreading and proliferation, so they can be considered suitable for designed aims.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic. Proks@imc.cas.cz.
authors.forename: A
authors.initials: A
authors.lastname: Golunova
authors.forename: J
authors.initials: J
authors.lastname: Jaroš
authors.forename: V
authors.initials: V
authors.lastname: Jurtíková
authors.forename: I
authors.initials: I
authors.lastname: Kotelnikov
authors.forename: J
authors.initials: J
authors.lastname: Kotek
authors.forename: H
authors.initials: H
authors.lastname: Hlídková
authors.forename: L
authors.initials: L
authors.lastname: Streit
authors.forename: A
authors.initials: A
authors.lastname: Hampl
authors.forename: F
authors.initials: F
authors.lastname: Rypáček
authors.forename: V
authors.initials: V
authors.lastname: Proks
mesh_terms: D000178:Acrylamides; D017667:Adipocytes; D001672:Biocompatible Materials; D032701:Biomimetics; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D059985:Cryogels; D004548:Elasticity; D006801:Humans; D016062:Porosity; D013234:Stem Cells; D054457:Tissue Scaffolds; D014867:Water / Q000737:chemistry
chemical_list: D000178:Acrylamides; D001672:Biocompatible Materials; D059985:Cryogels; D014867:Water; C032976:N-(2-hydroxypropyl)methacrylamide