title: Role of mannose-binding lectin in intestinal homeostasis and fungal elimination.
abstract: Mannose-binding lectin (MBL) is a soluble lectin of the innate immune system that is produced by the liver and secreted into the circulation where it activates the lectin complement pathway, enhances phagocytosis of microorganisms by leukocytes, and modulates inflammation. MBL can recognize patterns on the surface of different pathogens, including Candida albicans. Our aims were to investigate whether MBL is expressed in the gut epithelium and to examine its effect on the modulation of intestinal inflammation and C. albicans elimination. Using reverse transcriptase-PCR, MBL transcripts were highly expressed in different parts of the mouse gut. MBL expression was also detected by immunoblotting and immunolocalization in response to C. albicans colonization of the gut; the highest expression of MBL was detected in the stomach. Blocking MBL by administering mannans to mice increased C. albicans colonization. MBL-deficient mice had a higher level of colonization than wild-type mice. Dextran sodium sulfate-induced colitis promoted C. albicans dissemination to the kidneys and lungs of MBL-deficient mice. MBL-deficient mice exhibited elevated expression of interleukin (IL)-17, IL-23, dectin-1, and Toll-like receptor-4. This study shows that MBL expression is induced in the gut in response to C. albicans sensing and is required for intestinal homeostasis and host defense against C. albicans.
authors.affiliation: Université Lille Nord de France, Lille, France.|UDSL, Lille, France.|INSERM U995, Lille, France.|CHRU Lille, Lille, France.
authors.forename: L
authors.initials: L
authors.lastname: Choteau
authors.affiliation: Université Lille Nord de France, Lille, France.|UDSL, Lille, France.|INSERM U995, Lille, France.
authors.forename: M
authors.initials: M
authors.lastname: Parny
authors.affiliation: Service de Parasitologie Mycologie, Pole de Biologie Pathologie Génétique, Lille, France.
authors.forename: N
authors.initials: N
authors.lastname: François
authors.affiliation: Université Lille Nord de France, Lille, France.|UDSL, Lille, France.|INSERM U995, Lille, France.
authors.forename: B
authors.initials: B
authors.lastname: Bertin
authors.affiliation: Université Lille Nord de France, Lille, France.|UDSL, Lille, France.|INSERM U995, Lille, France.
authors.forename: M
authors.initials: M
authors.lastname: Fumery
authors.affiliation: Université Lille Nord de France, Lille, France.|UDSL, Lille, France.|INSERM U995, Lille, France.
authors.forename: L
authors.initials: L
authors.lastname: Dubuquoy
authors.affiliation: Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: Department of Gastroenterology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: J-F
authors.initials: JF
authors.lastname: Colombel
authors.affiliation: Université Lille Nord de France, Lille, France.|UDSL, Lille, France.|INSERM U995, Lille, France.
authors.forename: T
authors.initials: T
authors.lastname: Jouault
authors.affiliation: Université Lille Nord de France, Lille, France.|UDSL, Lille, France.|INSERM U995, Lille, France.|CHRU Lille, Lille, France.
authors.forename: D
authors.initials: D
authors.lastname: Poulain
authors.affiliation: Université Lille Nord de France, Lille, France.|UDSL, Lille, France.|INSERM U995, Lille, France.|CHRU Lille, Lille, France.
authors.forename: B
authors.initials: B
authors.lastname: Sendid
authors.affiliation: Université Lille Nord de France, Lille, France.|UDSL, Lille, France.|INSERM U995, Lille, France.
authors.forename: S
authors.initials: S
authors.lastname: Jawhara
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology*; D002478:Cells, Cultured; D003092:Colitis / Q000276:immunology*; D037582:Complement Pathway, Mannose-Binding Lectin; D016264:Dextran Sulfate; D005260:Female; D006706:Homeostasis; D054884:Host-Pathogen Interactions; D006801:Humans; D018928:Immunity, Mucosal; D020381:Interleukin-17 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D037601:Mannose-Binding Lectin / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010587:Phagocytosis; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: D020381:Interleukin-17; D037601:Mannose-Binding Lectin; C445639:Mbl2 protein, mouse; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; C069858:mannose binding protein A; D016264:Dextran Sulfate