title: Metabolic changes in rat urine after acute paraquat poisoning and discriminated by support vector machine.
abstract: Paraquat is quick-acting and non-selective, killing green plant tissue on contact; it is also toxic to human beings and animals. In this study, we developed a urine metabonomic method by gas chromatography-mass spectrometry to evaluate the effect of acute paraquat poisoning on rats. Pattern recognition analysis, including both partial least squares discriminate analysis and principal component analysis revealed that acute paraquat poisoning induced metabolic perturbations. Compared with the control group, the levels of benzeneacetic acid and hexadecanoic acid of the acute paraquat poisoning group (intragastric administration 36 mg/kg) increased, while the levels of butanedioic acid, pentanedioic acid, altronic acid decreased. Based on these urinary metabolomics data, support vector machine was applied to discriminate the metabolomic change of paraquat groups from the control group, which achieved 100% classification accuracy. In conclusion, metabonomic method combined with support vector machine can be used as a useful diagnostic tool in paraquat-poisoned rats.
authors.affiliation: Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Wen
authors.affiliation: The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhang
authors.affiliation: The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, Lishui, 323000, China.
authors.forename: Shuanghu
authors.initials: S
authors.lastname: Wang
authors.affiliation: The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, Lishui, 323000, China.
authors.forename: Peiwu
authors.initials: P
authors.lastname: Geng
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Fa
authors.initials: F
authors.lastname: Sun
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Mengchun
authors.initials: M
authors.lastname: Chen
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Guanyang
authors.initials: G
authors.lastname: Lin
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Lufeng
authors.initials: L
authors.lastname: Hu
authors.affiliation: Function Experiment Teaching Center of Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Jianshe
authors.initials: J
authors.lastname: Ma
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D055432:Metabolomics / Q000379:methods*; D010269:Paraquat / Q000506:poisoning* / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D060388:Support Vector Machine; D014556:Urine / Q000737:chemistry*
chemical_list: D010269:Paraquat
keywords: GC-MS; metabolomics; paraquat; poisoning; rat