title: Geriatric Respondents and Non-Respondents to Probiotic Intervention Can be Differentiated by Inherent Gut Microbiome Composition.
abstract: METHODS
  Probiotic interventions are known to have been shown to influence the composition of the intestinal microbiota in geriatrics. The growing concern is the apparent variation in response to identical strain dosage among human volunteers. One factor that governs this variation is the host gut microbiome. In this study, we attempted to define a core gut metagenome, which could act as a predisposition signature marker of inherent bacterial community that can help predict the success of a probiotic intervention.
  
  
  RESULTS
  To characterize the geriatric gut microbiome, we designed primers targeting the 16S rRNA hypervariable region V2-V3 followed by semiconductor sequencing using Ion Torrent PGM. Among respondents and non-respondents, the chief genera of phylum Firmicutes that showed significant differences are Lactobacillus, Clostridium, Eubacterium, and Blautia (q < 0.002), while in the genera of phylum Proteobacteria included Shigella, Escherichia, Burkholderia and Camphylobacter (q < 0.002).
  
  
  CONCLUSIONS
  We have identified potential microbial biomarkers and taxonomic patterns that correlate with a positive response to probiotic intervention in geriatric volunteers. Future work with larger cohorts of geriatrics with diverse dietary influences could reveal the potential of the signature patterns of microbiota for personalized nutrition.
authors.affiliation: Department of Dairy Science, South Dakota State University , Brookings, SD , USA.
authors.forename: Suja
authors.initials: S
authors.lastname: Senan
authors.affiliation: Department of Dairy Microbiology, Anand Agricultural University , Anand , India.
authors.forename: Jashbhai B
authors.initials: JB
authors.lastname: Prajapati
authors.affiliation: Department of Animal Biotechnology, Anand Agricultural University , Anand , India.
authors.forename: Chaitanya G
authors.initials: CG
authors.lastname: Joshi
authors.affiliation: Department of Dairy Microbiology, Anand Agricultural University , Anand , India.
authors.forename: V
authors.initials: V
authors.lastname: Sreeja
authors.affiliation: Department of Community Medicine, H. M Patel Center for Medical Care and Education , Karamsad , India.
authors.forename: Manisha K
authors.initials: MK
authors.lastname: Gohel
authors.affiliation: Department of Microbiology, H. M Patel Center for Medical Care and Education , Karamsad , India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Trivedi
authors.affiliation: Department of Microbiology, H. M Patel Center for Medical Care and Education , Karamsad , India.
authors.forename: Rupal M
authors.initials: RM
authors.lastname: Patel
authors.affiliation: Department of Medicine, H. M Patel Center for Medical Care and Education , Karamsad , India.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Pandya
authors.affiliation: Department of Community Medicine, H. M Patel Center for Medical Care and Education , Karamsad , India.
authors.forename: Uday Shankar
authors.initials: US
authors.lastname: Singh
authors.affiliation: Central Research Services, Charutar Arogya Mandal , Karamsad , India.
authors.forename: Ajay
authors.initials: A
authors.lastname: Phatak
authors.affiliation: Department of Dairy Science, South Dakota State University , Brookings, SD , USA.
authors.forename: Hasmukh A
authors.initials: HA
authors.lastname: Patel
keywords: MTCC 5463; geriatric; gut; metagenome; probiotics