title: Copy number increase of oncoprotein CIP2A is associated with poor patient survival in human head and neck squamous cell carcinoma.
abstract: BACKGROUND
  CIP2A, an inhibitor of PP2A tumour suppressor function, is a widely overexpressed biomarker of aggressive disease and poor therapy response in multiple human cancer types.
  
  
  METHODS
  CIP2A and DPPA4 copy number alterations and expression were analysed by fluorescence in situ hybridisation (FISH) and immunohistochemistry (IHC) in different cell lines and a tissue microarray of 52 HNSCC patients. Results were correlated with patient survival and other clinicopathological data.
  
  
  RESULTS
  CIP2A and DPPA4 copy number increase occurred at a relatively high frequency in human HNSCC patient samples. CIP2A but not DPPA4 FISH status was significantly associated with patient survival. CIP2A detection by combining IHC with FISH yielded superior resolution in the prognostication of HNSCC.
  
  
  CONCLUSIONS
  CIP2A copy number increase is associated with poor patient survival in human HNSCC. We suggest that the reliability and prognostic value of CIP2A detection can be improved by performing FISH analysis to CIP2A IHC positive tumours.
authors.affiliation: Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
authors.forename: Johannes
authors.initials: J
authors.lastname: Routila
authors.affiliation: Prostate Cancer Research Center, Institute of Biosciences and Medical Technology-BioMediTech, University of Tampere, Tampere, Finland.|Research and Application Centre for Scientific and Technological Investigations (NABILTEM), Namik Kemal University, Tekirdag, Turkey.
authors.forename: Türker
authors.initials: T
authors.lastname: Bilgen
authors.affiliation: Prostate Cancer Research Center, Institute of Biosciences and Medical Technology-BioMediTech, University of Tampere, Tampere, Finland.|Fimlab Laboratories, Tampere University Hospital, Tampere, Finland.
authors.forename: Outi
authors.initials: O
authors.lastname: Saramäki
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, Turku, Finland.
authors.forename: Reidar
authors.initials: R
authors.lastname: Grénman
authors.affiliation: Prostate Cancer Research Center, Institute of Biosciences and Medical Technology-BioMediTech, University of Tampere, Tampere, Finland.|Fimlab Laboratories, Tampere University Hospital, Tampere, Finland.
authors.forename: Tapio
authors.initials: T
authors.lastname: Visakorpi
authors.affiliation: Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.|Department of Pathology, University of Turku, Turku, Finland.
authors.forename: Jukka
authors.initials: J
authors.lastname: Westermarck
authors.affiliation: Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.|Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, Turku, Finland.
authors.forename: Sami
authors.initials: S
authors.lastname: Ventelä
mesh_terms: D001324:Autoantigens / Q000096:biosynthesis* / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D018572:Disease-Free Survival; D005260:Female; D018628:Gene Dosage; D006367:HeLa Cells; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics*; D008875:Middle Aged; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics; D011379:Prognosis; D015203:Reproducibility of Results; D000077195:Squamous Cell Carcinoma of Head and Neck; D046888:Tissue Array Analysis / Q000379:methods
chemical_list: D001324:Autoantigens; D014408:Biomarkers, Tumor; C500266:CIP2A protein, human; C000611651:DPPA4 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins
keywords: CIP2A; DPPA4; head and neck; in situ hybridisation; protein phosphatase 2a; squamous cell carcinoma