title: Recombinant adenovirus encoding NLRP3 RNAi attenuate inflammation and brain injury after intracerebral hemorrhage.
abstract: Numerous evidence have shown that microglia mediated inflammation plays a pivotal role in the development of brain injury after intracerebral hemorrhage (ICH). Therefore anti-inflammation therapy represents a potentially promising approach to ICH. Recently, NLRP3 inflammasome was discovered to facilitate the inflammatory response. However, the effect of NLRP3 inflammasome after ICH has not been fully studied. To explore the potential of NLRP3 inflammasome, we detected NLRP3 expression, inflammation, brain edema and neurological functions in vitro and in vivo. We found that ICH activated the NLRP3 inflammasome and inflammation. However, NLRP3 RNAi could attenuate inflammation and brain injury after ICH. Therefore, the findings suggested that recombinant adenovirus encoding NLRP3 RNAi might be valuable as a potential strategy for anti-inflammation therapy in ICH.
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian 350025, China.
authors.forename: Bangqing
authors.initials: B
authors.lastname: Yuan
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian 350025, China.
authors.forename: Hanchao
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian 350025, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian 350025, China.
authors.forename: Tonggang
authors.initials: T
authors.lastname: Su
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical, University, Chongqing 402160, China.
authors.forename: Shanchuan
authors.initials: S
authors.lastname: Zhong
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical, University, Chongqing 402160, China. Electronic address: yangzhao5140@sohu.com.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001929:Brain Edema / Q000209:etiology; D001930:Brain Injuries / Q000188:drug therapy*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D020169:Caspases / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D002543:Cerebral Hemorrhage / Q000150:complications*; D004195:Disease Models, Animal; D004622:Embryo, Mammalian; D007249:Inflammation / Q000188:drug therapy* / Q000209:etiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000187:drug effects; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D009474:Neurons / Q000187:drug effects; D034622:RNA Interference / Q000502:physiology*; D014161:Transduction, Genetic
chemical_list: D002352:Carrier Proteins; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C492716:Nlrp3 protein, mouse; D020169:Caspases
keywords: Inflammation; Intracerebral hemorrhage; NLRP3 RNAi