title: Quantitative proteomic analysis of Araucaria angustifolia (Bertol.) Kuntze cell lines with contrasting embryogenic potential.
abstract: GeLC–MS/MS based label free proteomic profiling was used in the large scale identification and quantification of proteins from Brazilian pine (Araucaria angustifolia) embryogenic cell (EC) lines that showed different propensities to form somatic embryos. Using a predicted protein sequence database that was derived from A. angustifolia RNA-Seq data, 2398 non-redundant proteins were identified. The log2 of the spectral count values of 858 proteins of these proteins showed a normal distribution, and were used for statistical analysis. Statistical tests indicated that 106 proteins were significantly differentially abundant between the two EC lines, and that 35 were more abundant in the responsive genotype (EC line SE1) and 71 were more abundant in the blocked genotype (EC line SE6). An increase in the abundance of proteins related to cell defense, anti-oxidative stress responses, and storage reserve deposition was observed in SE1. Moreover, in SE6 we observed an increased abundance of two proteins associated with seed development during the embryogenic cell proliferation stage, which we suggest is associated with genotypes showing a low responsiveness to embryo formation. Differences in protein abundance between the EC lines are discussed in terms of carbohydrate metabolism, cell division, defense response, gene expression, and response to reactive oxygen species.
authors.affiliation: Department of Botany, Institute of Bioscience, University of São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: André Luis Wendt
authors.initials: AL
authors.lastname: dos Santos
authors.affiliation: Department of Botany, Institute of Bioscience, University of São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: Paula
authors.initials: P
authors.lastname: Elbl
authors.affiliation: Department of Botany, Institute of Bioscience, University of São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: Bruno Viana
authors.initials: BV
authors.lastname: Navarro
authors.affiliation: Department of Botany, Institute of Bioscience, University of São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: Leandro Francisco
authors.initials: LF
authors.lastname: de Oliveira
authors.affiliation: Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Salvato
authors.affiliation: College of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal, São Paulo, Brazil.
authors.forename: Tiago Santana
authors.initials: TS
authors.lastname: Balbuena
authors.affiliation: Department of Botany, Institute of Bioscience, University of São Paulo, São Paulo, São Paulo, Brazil. Electronic address: enyfloh@usp.br.
authors.forename: Eny Iochevet Segal
authors.initials: EI
authors.lastname: Floh
mesh_terms: D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation; D005838:Genotype; D010940:Plant Proteins / Q000378:metabolism*; D058439:Plant Somatic Embryogenesis Techniques; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D012313:RNA / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D017423:Sequence Analysis, RNA; D053719:Tandem Mass Spectrometry; D064028:Tracheophyta / Q000378:metabolism*; D014357:Trypsin / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D010940:Plant Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; D012313:RNA; D014357:Trypsin
keywords: Conifer; Embryogenic potential; Molecular markers; Quantitative proteomics; Somatic embryogenesis