title: [Diagnostic Significance of BAT in Anaphylaxis to Non-ionic Contrast Media].
abstract: OBJECTIVE
  To investigate the diagnostic significance of basophil activation test (BAT) in anaphylaxis to non-ionic contrast media through testing the content of CD63, mast cell-carboxypeptidase A3 (MC-CPA3), and terminal complement complex SC5b-9 of the individuals by testing their levels in the normal immune group and the anaphylaxis groups to β-lactam drugs and non -ionic contrast media.
  
  
  METHODS
  The CD63 expression of basophilic granulocyte in blood was detected by flow cytometry. The levels of MC-CPA3 in blood serum and SC5b-9 in blood plasma were detected by ELISA.
  
  
  RESULTS
  The CD63 expression of basophilic granulocyte in blood, the levels of MC-CPA3 and SC5b-9 of anaphylaxis to non-ionic contrast media and β-lactam drugs were significantly higher than that in normal immune group (P < 0.05).
  
  
  CONCLUSIONS
  There is activation of basophilic granulocytes, mast cells and complement system in anaphylaxis to non-ionic contrast media. BAT can be used to diagnose the anaphylaxis to non-ionic contrast media.
authors.forename: Hao-yue
authors.initials: HY
authors.lastname: Zhang
authors.forename: Su-jun
authors.initials: SJ
authors.lastname: Xu
authors.forename: Xiao-xian
authors.initials: XX
authors.lastname: Tang
authors.forename: Ji-jun
authors.initials: JJ
authors.lastname: Niu
authors.forename: Xiang-jie
authors.initials: XJ
authors.lastname: Guo
authors.forename: Cai-rong
authors.initials: CR
authors.lastname: Gao
mesh_terms: D000707:Anaphylaxis / Q000175:diagnosis*; D001491:Basophils / Q000166:cytology*; D043422:Carboxypeptidases A / Q000378:metabolism; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D003287:Contrast Media; D005434:Flow Cytometry; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D008407:Mast Cells / Q000166:cytology; D060149:Tetraspanin 30 / Q000378:metabolism
chemical_list: D015938:Complement Membrane Attack Complex; D003287:Contrast Media; D060149:Tetraspanin 30; C468648:CPA3 protein, human; D043422:Carboxypeptidases A