title: Delivery of human mesenchymal adipose-derived stem cells restores multiple urological dysfunctions in a rat model mimicking radical prostatectomy damages through tissue-specific paracrine mechanisms.
abstract: Urinary incontinence (UI) and erectile dysfunction (ED) are the most common functional urological disorders and the main sequels of radical prostatectomy (RP) for prostate cancer. Mesenchymal stem cell (MSC) therapy holds promise for repairing tissue damage due to RP. Because animal studies accurately replicating post-RP clinical UI and ED are lacking, little is known about the mechanisms underlying the urological benefits of MSC in this setting. To determine whether and by which mechanisms MSC can repair damages to both striated urethral sphincter (SUS) and penis in the same animal, we delivered human multipotent adipose stem cells, used as MSC model, in an immunocompetent rat model replicating post-RP UI and ED. In this model, we demonstrated by using noninvasive methods in the same animal from day 7 to day 90 post-RP injury that MSC administration into both the SUS and the penis significantly improved urinary continence and erectile function. The regenerative effects of MSC therapy were not due to transdifferentiation and robust engraftment at injection sites. Rather, our results suggest that MSC benefits in both target organs may involve a paracrine process with not only soluble factor release by the MSC but also activation of the recipient's secretome. These two effects of MSC varied across target tissues and damaged-cell types. In conclusion, our work provides new insights into the regenerative properties of MSC and supports the ability of MSC from a single source to repair multiple types of damage, such as those seen after RP, in the same individual.
authors.affiliation: INSERM U955 Team 12, Créteil, France.|Université Paris-Est Créteil, UMR_S955, UPEC, Créteil, France.|Urology Department, APHP, Hôpital H. Mondor-A. Chenevier, Créteil, France.
authors.forename: René
authors.initials: R
authors.lastname: Yiou
authors.affiliation: INSERM U955 Team 12, Créteil, France.|Université Paris-Est Créteil, UMR_S955, UPEC, Créteil, France.
authors.forename: Meriem
authors.initials: M
authors.lastname: Mahrouf-Yorgov
authors.affiliation: INSERM U955 Team 12, Créteil, France.|Université Paris-Est Créteil, UMR_S955, UPEC, Créteil, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Trébeau
authors.affiliation: INSERM U955 Team 12, Créteil, France.|Université Paris-Est Créteil, UMR_S955, UPEC, Créteil, France.|Urology Department, APHP, Hôpital H. Mondor-A. Chenevier, Créteil, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Zanaty
authors.affiliation: INSERM U955 Team 12, Créteil, France.|Université Paris-Est Créteil, UMR_S955, UPEC, Créteil, France.|Plateforme Exploration Fonctionnelle du Petit Animal EPFA01 Mondor Institute, Créteil, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Lecointe
authors.affiliation: INSERM U955 Team 12, Créteil, France.|Université Paris-Est Créteil, UMR_S955, UPEC, Créteil, France.|Plateforme Exploration Fonctionnelle du Petit Animal EPFA01 Mondor Institute, Créteil, France.
authors.forename: Richard
authors.initials: R
authors.lastname: Souktani
authors.affiliation: INSERM U955 Team 12, Créteil, France.|Université Paris-Est Créteil, UMR_S955, UPEC, Créteil, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Zadigue
authors.affiliation: INSERM U955 Team 12, Créteil, France.|Université Paris-Est Créteil, UMR_S955, UPEC, Créteil, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Figeac
authors.affiliation: INSERM U955 Team 12, Créteil, France.|Université Paris-Est Créteil, UMR_S955, UPEC, Créteil, France.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Rodriguez
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D004195:Disease Models, Animal; D064593:Heterografts; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D019899:Paracrine Communication; D011183:Postoperative Complications / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D011468:Prostatectomy / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014521:Urethra / Q000378:metabolism / Q000473:pathology
keywords: Cell therapy; Erectile dysfunction; Mesenchymal stem cells; Radical prostatectomy; Urinary incontinence