title: Studies regarding the protective effects exhibited by antidepressants on cell models.
abstract: The study aimed to assess in vitro the short-term effects exerted by fluoxetine, sertraline and venlafaxine on certain physiological properties in two different study models: U937 monocytes and erythrocytes isolated from patients treated with the above-mentioned molecules. Results on U937 cell suspensions revealed the depolarization of the cell membrane induced by the three antidepressants. The maximal depolarization effect was registered after 15 minutes of cell exposure and was concentration-dependent, in a non-monotonic manner. The effect was also dependent on the tested compound, fluoxetine presenting the strongest depolarizing effect compared to sertraline and venlafaxine. The erythrocyte susceptibility to lipid peroxidation and glucose-6-phosphate dehydrogenase activity were assessed on red blood cells isolated from patients with depressive disorder. Our results revealed that antidepressant treatment induced the antioxidant defense, by decreasing erythrocyte susceptibility to lipid peroxidation and increasing glucose-6-phosphate dehydrogenase activity. The effect is more intense in the case of severe pathology and less evident in the case of moderate or minor disorder, as expressed by MADRS (Montgomery-Åsberg Depression Rating Scale) score. Our results could indicate that selected antidepressants at therapeutic concentrations, besides their known pharmacological effects, exhibit a protective effect against oxidative stress and also influence cells with immune properties.
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania; m16ilie@yahoo.com.
authors.forename: Nicoleta Carmen
authors.initials: NC
authors.lastname: Purdel
authors.forename: Rucsandra
authors.initials: R
authors.lastname: Dănciulescu Miulescu
authors.forename: Marius Cristian
authors.initials: MC
authors.lastname: Neamţu
authors.forename: Elena Taina
authors.initials: ET
authors.lastname: Avramescu
authors.forename: Mihaela
authors.initials: M
authors.lastname: Ilie
authors.forename: Gina
authors.initials: G
authors.lastname: Manda
authors.forename: Denisa Marilena
authors.initials: DM
authors.lastname: Margină
mesh_terms: D000928:Antidepressive Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry; D002462:Cell Membrane / Q000187:drug effects*; D003866:Depressive Disorder / Q000188:drug therapy; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects*; D005260:Female; D005473:Fluoxetine / Q000494:pharmacology; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry; D006801:Humans; D007107:Immune System; D015227:Lipid Peroxidation; D008875:Middle Aged; D018384:Oxidative Stress; D020280:Sertraline / Q000494:pharmacology; D020298:U937 Cells / Q000187:drug effects; D000069470:Venlafaxine Hydrochloride / Q000494:pharmacology
chemical_list: D000928:Antidepressive Agents; D000975:Antioxidants; D005473:Fluoxetine; D000069470:Venlafaxine Hydrochloride; D005954:Glucosephosphate Dehydrogenase; D020280:Sertraline