title: Simultaneous conversion of glucose and xylose to 3-hydroxypropionic acid in engineered Escherichia coli by modulation of sugar transport and glycerol synthesis.
abstract: Escherichia coli expressing the Lactobacillus brevis dhaB1B2B3 and dhaR1R2 clusters and Pseudomonas aeruginosa aldhH was engineered to produce 3-HP from glucose and xylose via the glycerol biosynthetic pathway. Glycerol, a key precursor for 3-HP biosynthesis was produced by overexpression of the GPD1 and GPP2 genes from Saccharomyces cerevisiae. For relief of carbon catabolite repression, deletion of the chromosomal ptsG gene and overexpression of the endogenous xylR gene rendered engineered E. coli JHS01300/pCPaGGRm to utilize glucose and xylose simultaneously and to produce glycerol at 0.48 g/g yield and 0.35 g/L-h productivity. Finally, engineered E. coli JHS01300/pELDRR+pCPaGGRm produced 29.4 g/L of 3-HP with 0.54 g/L-h productivity and 0.36 g/g yield in a sugar-limited fed-batch fermentation. It was concluded that dual modulation of sugar transport and glycerol biosynthesis is a promising strategy for efficient conversion of glucose and xylose to 3-HP.
authors.affiliation: Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: In-Young
authors.initials: IY
authors.lastname: Jung
authors.affiliation: Interdisciplinary Program of Bioengineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jong-Won
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Won-Ki
authors.initials: WK
authors.lastname: Min
authors.affiliation: Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 136-702, Republic of Korea.
authors.forename: Yong-Cheol
authors.initials: YC
authors.lastname: Park
authors.affiliation: Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul 151-742, Republic of Korea; Interdisciplinary Program of Bioengineering, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: jhseo94@snu.ac.kr.
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Seo
mesh_terms: D050260:Carbohydrate Metabolism / Q000235:genetics; D057465:Catabolite Repression; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering / Q000379:methods*; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+) / Q000235:genetics / Q000378:metabolism; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D052196:Levilactobacillus brevis / Q000235:genetics / Q000378:metabolism; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D019344:Lactic Acid; D014994:Xylose; C031601:hydracrylic acid; C568531:GPD1 protein, S cerevisiae; C568532:GPD2 protein, S cerevisiae; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C031629:phosphoenolpyruvate-glucose phosphotransferase; D005947:Glucose; D005990:Glycerol
keywords: 3-Hydroxypropionic acid; Glucose; Xylose; ptsG; xylR