title: Production of Epitope-Specific Antibodies by Immunization with Synthetic Epitope Peptide Formulated with CpG-DNA-Liposome Complex Without Carriers.
abstract: Antibody production using synthetic peptides has been investigated extensively to develop therapeutic antibodies and prophylactic vaccines. Previously, we reported that a complex of CpG-DNA and synthetic peptides corresponding to B cell epitopes, encapsulated in a phosphatidyl-β-oleoyl-γ-palmitoyl ethanolamine (DOPE):cholesterol hemisuccinate (CHEMS) complex, significantly enhanced the synthetic peptide-specific IgG production. Here, we describe synthetic peptide-based epitope screening and antibody production without conventional carriers.
authors.affiliation: Department of Microbiology, College of Medicine, Hallym University, 39 Hallymdaehak-gil, Gangwon-do, 200-702, Republic of Korea.
authors.forename: Dongbum
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Chungbuk, Republic of Korea.
authors.forename: Younghee
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Microbiology, College of Medicine, Hallym University, 39 Hallymdaehak-gil, Gangwon-do, 200-702, Republic of Korea. hjookwon@hallym.ac.kr.|Center for Medical Science Research, College of Medicine, Hallym University, Gangwon-do, Republic of Korea. hjookwon@hallym.ac.kr.
authors.forename: Hyung-Joo
authors.initials: HJ
authors.lastname: Kwon
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000008:administration & dosage / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte / Q000008:administration & dosage / Q000276:immunology; D007114:Immunization; D008081:Liposomes; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage; D010455:Peptides / Q000008:administration & dosage / Q000138:chemical synthesis / Q000276:immunology*
chemical_list: D000906:Antibodies; D000939:Epitopes; D018985:Epitopes, B-Lymphocyte; D008081:Liposomes; D009838:Oligodeoxyribonucleotides; D010455:Peptides
keywords: Antibody production; CpG-DNA; CpG-DNA-liposome complex; Epitope; Peptide