title: Thiol-ene Photocrosslinking of Cytocompatible Resilin-Like Polypeptide-PEG Hydrogels.
abstract: A range of chemical strategies have been used for crosslinking recombinant polypeptide hydrogels, although only a few have employed photocrosslinking approaches. Here, we capitalize on the novel insect protein, resilin, and the versatility of click reactions to introduce a resilin-like polypeptide (RLP) that is capable of photoinitiated thiol-ene crosslinking. Lysine residues of the RLP were functionalized with norbornene acid as confirmed via 1H-NMR spectroscopy. The RLPNs were subsequently photocrosslinked with multi-arm PEG thiols in the presence of a photoinitiator to form elastic hybrid hydrogels. The crosslinking reaction and resulting RLP-PEG networks demonstrated cytocompatibility with human mesenchymal stem cells in both 2D cell-adhesion and 3D photoencapsulation studies.
authors.affiliation: Department of Materials Science and Engineering, University of Delaware, Newark, Delaware 19716, United States.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: McGann
authors.affiliation: Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, United States.
authors.forename: Rebekah E
authors.initials: RE
authors.lastname: Dumm
authors.affiliation: Department of Materials Science and Engineering, University of Delaware, Newark, Delaware 19716, United States.
authors.forename: Anna K
authors.initials: AK
authors.lastname: Jurusik
authors.affiliation: Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, United States.
authors.forename: Ishnoor
authors.initials: I
authors.lastname: Sidhu
authors.affiliation: Department of Biomedical Engineering, University of Delaware, Newark, Delaware 19716, United States. kiick@udel.edu.|Delaware Biotechnology Institute, 15 Innovation Way, Newark, Delaware 19711, United States. kiick@udel.edu.|Department of Materials Science and Engineering, University of Delaware, Newark, Delaware 19716, United States. kiick@udel.edu.
authors.forename: Kristi L
authors.initials: KL
authors.lastname: Kiick
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion; D057930:Click Chemistry; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry*; D019476:Insect Proteins; D059630:Mesenchymal Stem Cells; D010455:Peptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D019476:Insect Proteins; D010455:Peptides; D011092:Polyethylene Glycols; C030951:resilin
keywords: biomaterials; hydrogels; photocrosslinking; protein; thiol-ene