title: Cytokine (interleukin-1beta) and MMP levels in gingival crevicular fluid after use of platelet-rich fibrin or connective tissue graft in the treatment of localized gingival recessions.
abstract: OBJECTIVE
  The objective of this study was to evaluate the levels of MMP-8, MMP-9, TIMP-1 and interleukin-1beta (IL-1β) in gingival crevicular fluid during the early and late stages of healing in gingival recession sites treated with coronally advanced flap plus platelet-rich fibrin (CAF+PRF) compared with CAF plus connective tissue graft (CAF+CTG).
  
  
  METHODS
  Twenty-four nonsmoking patients with Miller Class I or Class II localized gingival recession defects in bilateral sites received treatment with either CAF+PRF (PRF group) or CAF+CTG (CTG group). Gingival crevicular fluid samples were collected at baseline and at 10 d and 1 mo, 3 mo and 6 mo after surgery. The levels of MMP-8, MMP-9, TIMP-1 and IL-1β in gingival crevicular fluid were measured using a time-resolved immunofluorometric assay and ELISAs.
  
  
  RESULTS
  Gingival crevicular fluid levels of IL-1β were significantly elevated in the CTG group at 10 d compared with baseline (p < 0.05). At 10 d after surgery, the levels of TIMP-1 in gingival crevicular fluid showed a significant decrease in the CTG group compared with the PRF group (p < 0.05). The levels of IL-1β and MMP-8 in gingival crevicular fluid were significantly lower in the PRF group than in the CTG group at 10 d (p < 0.05). No significant differences were found in all clinical and biochemical parameters at 1, 3, and 6 mo between study groups (p > 0.05).
  
  
  CONCLUSIONS
  Root coverage with CAF+PRF has a significant effect on increasing gingival crevicular fluid TIMP-1 levels and suppressing gingival crevicular fluid MMP-8 and IL-β levels at 10 d. Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-1 and IL-1β did not seem to be affected by the technique at later phases of wound healing.
authors.affiliation: Department of Periodontology, School of Dentistry, Ege University, Bornova-İzmir, Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Eren
authors.affiliation: Department of Oral and Maxillofacial Diseases, Helsinki University and Helsinki University Hospital, Helsinki, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Tervahartiala
authors.affiliation: Department of Oral and Maxillofacial Diseases, Helsinki University and Helsinki University Hospital, Helsinki, Finland.|Division of Periodontology, Department of Dental Medicine, Karolinska Institute, Huddinge, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Sorsa
authors.affiliation: Department of Periodontology, School of Dentistry, Ege University, Bornova-İzmir, Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Atilla
mesh_terms: D003238:Connective Tissue / Q000637:transplantation*; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005889:Gingival Recession / Q000473:pathology / Q000601:surgery / Q000628:therapy*; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis*; D020784:Matrix Metalloproteinase 8 / Q000032:analysis*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D000073183:Platelet-Rich Fibrin; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis*
chemical_list: D053583:Interleukin-1beta; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9
keywords: connective tissue; cytokine; fibrin; gingival crevicular fluid; gingival recession; matrix metalloproteinases