title: Synthesis and evaluation of the biostability and cell compatibility of novel conjugates of nucleobase, peptidic epitope, and saccharide.
abstract: This article reports the synthesis of a new class of conjugates containing a nucleobase, a peptidic epitope, and a saccharide and the evalution of their gelation, biostability, and cell compatibility. We demonstrate a facile synthetic process, based on solid-phase peptide synthesis of nucleopeptides, to connect a saccharide with the nucleopeptides for producing the target conjugates. All the conjugates themselves (1-8) display excellent solubility in water without forming hydrogels. However, a mixture of 5 and 8 self-assembles to form nanofibers and results in a supramolecular hydrogel. The proteolytic stabilities of the conjugates depend on the functional peptidic epitopes. We found that TTPV is proteolytic resistant and LGFNI is susceptible to proteolysis. In addition, all the conjugates are compatible to the mammalian cells tested.
authors.affiliation: Department of Chemistry, Brandeis University, 415 South Street, MS015, Waltham, MA 02453, USA.
authors.forename: Dan
authors.initials: D
authors.lastname: Yuan
authors.affiliation: Department of Chemistry, Brandeis University, 415 South Street, MS015, Waltham, MA 02453, USA.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Chemistry, Brandeis University, 415 South Street, MS015, Waltham, MA 02453, USA.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Chemistry, Brandeis University, 415 South Street, MS015, Waltham, MA 02453, USA.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Abdulgader Ahmed
authors.initials: AA
authors.lastname: Baoum
authors.affiliation: Department of Chemistry, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Khalid Omar
authors.initials: KO
authors.lastname: Al Footy
authors.affiliation: Department of Chemistry, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Khadija Omar
authors.initials: KO
authors.lastname: Badahdah
authors.affiliation: Department of Chemistry, Brandeis University, 415 South Street, MS015, Waltham, MA 02453, USA.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
keywords: biocompatibility; biostability; nucleobase; peptidic epitope; saccharide