title: Micronized cellular adipose matrix as a therapeutic injectable for diabetic ulcer.
abstract: BACKGROUND
  Despite the clinical potential of adipose-derived stem/stromal cells (ASCs), there are some clinical difficulties due to the regulation of cell therapies.
  
  
  METHODS
  Micronized cellular adipose matrix (MCAM) injectable was prepared through selective extraction of connective tissue fractions in fat tissue only through mechanical minimal manipulation procedures.
  
  
  RESULTS
  It retained some capillaries and ASCs, but most adipocytes were removed. The presence of viable ASCs, vascular endothelial cells was confirmed and ASCs of MCAM kept intact mesenchymal differentiation capacity. In diabetic mice, skin wounds treated with MCAM showed significantly accelerated healing compared with phosphate-buffered saline-treated ones.
  
  
  CONCLUSIONS
  The proven potential of MCAM to accelerate healing in ischemic diabetic ulcers may offer a simple, safe and minimally invasive means for tissue repair and revitalization.
authors.affiliation: Departments of Plastic Surgery, University of Tokyo Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-Ku, Tokyo 113-8655, Japan.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Feng
authors.affiliation: Departments of Plastic Surgery, University of Tokyo Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-Ku, Tokyo 113-8655, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Doi
authors.affiliation: Departments of Plastic Surgery, University of Tokyo Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-Ku, Tokyo 113-8655, Japan.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Kuno
authors.affiliation: Departments of Plastic Surgery, University of Tokyo Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-Ku, Tokyo 113-8655, Japan.
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Mineda
authors.affiliation: Departments of Plastic Surgery, University of Tokyo Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-Ku, Tokyo 113-8655, Japan.
authors.forename: Harunosuke
authors.initials: H
authors.lastname: Kato
authors.affiliation: Departments of Plastic Surgery, University of Tokyo Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-Ku, Tokyo 113-8655, Japan.
authors.forename: Kahori
authors.initials: K
authors.lastname: Kinoshita
authors.affiliation: Departments of Plastic Surgery, University of Tokyo Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-Ku, Tokyo 113-8655, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kanayama
authors.affiliation: Departments of Plastic Surgery, University of Tokyo Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-Ku, Tokyo 113-8655, Japan.
authors.forename: Takanobu
authors.initials: T
authors.lastname: Mashiko
authors.affiliation: Departments of Plastic Surgery, University of Tokyo Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-Ku, Tokyo 113-8655, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Yoshimura
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000637:transplantation*; D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D048909:Diabetes Complications / Q000628:therapy*; D003921:Diabetes Mellitus, Experimental; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007267:Injections; D037102:Lectins / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology*; D054457:Tissue Scaffolds; D014456:Ulcer / Q000628:therapy*; D014945:Wound Healing; D055815:Young Adult
chemical_list: D037102:Lectins
keywords: adipose stem cells; diabetic ulcer; extracellular matrix; flow cytometry; ischemia; lipoaspirates; minimal manipulation; tissue revitalization; vascular endothelial cells; wound healing