title: Multi-class method for biomonitoring of hair samples using gas chromatography-mass spectrometry.
abstract: Currently, non-invasive biomonitoring of human exposure to organic pollutants bases upon the analysis mainly of urine and human breast milk. While mostly persistent organic pollutants are the center of interest, the aim of our study was to develop a method for the determination of different chemical classes of emerging pollutants (organophosphorus flame retardants, plastic additives such as phthalates, bisphenol A, insecticides, antimicrobials, preservatives and musk fragrances) in hair by gas chromatography-mass spectrometry. The preferred sample preparation included hydrolysis of the hair with trifluoroacetic acid in methanol followed by a liquid-liquid extraction using hexane/ethyl acetate. The validated method is characterized by recoveries higher than 77 % for most analytes, relative standard deviations below 16 % and limits of detection between 2 pg mg(-1) (HHCB) and 292 pg mg(-1) (propylparaben) using 50 mg of dry hair. After respective blank corrections, bis-(2-ethylhexyl)phthalate (DEHP) and the musk fragrance HHCB were the predominant compounds determined in all hair samples at concentrations between 32 and 59 ng mg(-1) and 0.8-13 ng mg(-1), respectively. The bactericide triclosan and the insect repellent N,N-diethyl-3-methylbenzamide (DEET) were detected in selected hair samples at 2 and 0.8 ng mg(-1), respectively.
authors.affiliation: Department of Analytical Chemistry, Escuela Politécnica Superior, University of Seville, C/ Virgen de África 7, 41011, Seville, Spain.
authors.forename: Julia
authors.initials: J
authors.lastname: Martín
authors.affiliation: Department of Analytical Chemistry, Helmholtz Centre for Environmental Research - UFZ, Permoserstraße 15, 04318, Leipzig, Germany. monika.moeder@ufz.de.
authors.forename: Monika
authors.initials: M
authors.lastname: Möder
authors.affiliation: Institute of Laboratory Medicine, University of Leipzig, Liebigstraße 27b, 04103, Leipzig, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Gaudl
authors.affiliation: Department of Analytical Chemistry, Escuela Politécnica Superior, University of Seville, C/ Virgen de África 7, 41011, Seville, Spain.
authors.forename: Esteban
authors.initials: E
authors.lastname: Alonso
authors.affiliation: Department of Analytical Chemistry, Helmholtz Centre for Environmental Research - UFZ, Permoserstraße 15, 04318, Leipzig, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Reemtsma
mesh_terms: D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction / Q000379:methods
chemical_list: D004785:Environmental Pollutants
keywords: Emerging pollutants; Gas chromatography–mass spectrometry; Hair analysis; Human biomonitoring