title: miR-96 suppresses renal cell carcinoma invasion via downregulation of Ezrin expression.
abstract: BACKGROUND
  The present study examined the role of microRNA (miR)-96 in renal cell carcinoma (RCC) invasion.
  
  
  METHODS
  The expression of miR-96 was detected by quantitative reverse transcription-polymerase chain reaction in human RCC cell lines with high (Caki-1) and low (786-O) metastatic potential. Invasive ability and Ezrin expression were assessed in Caki-1 and 786-O cells transfected with a miR-96 mimic or inhibitor using wound healing assays, Transwell assays and western blotting. Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months.
  
  
  RESULTS
  miR-96 expression was significantly lower in Caki-1compared to786-O cells. The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic. Ezrin levels were negatively correlated with miR-96 in RCC, and inhibition of Ezrin expression suppressed the miR-96-induced change in invasive ability. The negative correlation between miR-96 and metastasis/Ezrin expression was also observed in human RCC specimens.
  
  
  CONCLUSIONS
  These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.
authors.affiliation: Urology Department, General Hospital of Jinan Military Command, 25 Shifan Road, Jinan, Shandong, 250031, China. yunengwang@hotmail.com.
authors.forename: Nengwang
authors.initials: N
authors.lastname: Yu
authors.affiliation: Shandong Cancer Hospital & Institute, Shandong Academy of Medical Sciences, 440 Jiyan Road, Jinan, 250117, China. 404815413@qq.com.
authors.forename: Shuai
authors.initials: S
authors.lastname: Fu
authors.affiliation: Department of urology, Beijing Tsinghua Changgung Hospital Medical Center, Tsinghua University, 168 Litang Road, Dongxiaokou Town, Changqing District, Beijing, 102218, China. justin7733@sina.com.
authors.forename: Yubao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Urology Department, Qilu Hospital, 107 West Wenhua Road, Jinan, Shandong, 250012, China. 58734622@qq.com.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Urology Department, General Hospital of Jinan Military Command, 25 Shifan Road, Jinan, Shandong, 250031, China. 13854111937@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Urology Department, General Hospital of Jinan Military Command, 25 Shifan Road, Jinan, Shandong, 250031, China. hjw.jnjz@126.com.
authors.forename: Junwen
authors.initials: J
authors.lastname: Hao
authors.affiliation: Oncology Department, General Hospital of Jinan Military Command, 25 Shifan Road, Jinan, Shandong, 250031, China. wangbaocheng1962@hotmail.com.
authors.forename: Baocheng
authors.initials: B
authors.lastname: Wang
authors.affiliation: Urology Department, General Hospital of Jinan Military Command, 25 Shifan Road, Jinan, Shandong, 250031, China. zhangaimin1967@hotmail.com.
authors.forename: Aimin
authors.initials: A
authors.lastname: Zhang
mesh_terms: D002292:Carcinoma, Renal Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000096:biosynthesis*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007668:Kidney / Q000473:pathology; D007680:Kidney Neoplasms / Q000473:pathology*; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D035683:MicroRNAs; D000099149:Ezrin; C541492:MIRN96 microRNA, human