title: A novel method for murine intrahepatic islet transplantation via cecal vein.
abstract: Islet transplantation is one of the most beneficial treatment modality to treat type 1 diabetic patients with frequent hypoglycemic unawareness. In clinical setting, human islets are infused via portal vein and are settled in the end-portal venules in the liver. However, mouse islets are transplanted into kidney subcapsule or liver through direct portal vein. These conventional transplantation methods have several drawbacks such as different physiological environments around the transplanted islets in kidney subcapsule from the liver and high mortality rate in direct portal vein approach. In this study, we introduced murine intrahepatic islet transplantation method via cecal vein to have the same surgical operation route in humans as well as guaranteeing low mortality rate after islet transplantation. With this protocol, consistent normoglycemia can be obtained in diabetic mice, while keeping operation-related mortality extremely low. This approach with easier accessibility and low mortality will make murine intrahepatic islet transplantation a useful model for studying immunological mechanisms such as strong innate and adaptive immune responses that occur in human islet transplantation.
authors.affiliation: Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Xenotransplantation Research Centre (XRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea. Electronic address: NR.Byun@gmail.com.
authors.forename: Nari
authors.initials: N
authors.lastname: Byun
authors.affiliation: Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Xenotransplantation Research Centre (XRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea. Electronic address: tte9801@hanmail.net.
authors.forename: Hyun-Je
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Xenotransplantation Research Centre (XRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea. Electronic address: byounghoon.min@gmail.com.
authors.forename: Byoung-Hoon
authors.initials: BH
authors.lastname: Min
authors.affiliation: Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Xenotransplantation Research Centre (XRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Institute for Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea. Electronic address: shinjs0309@gmail.com.
authors.forename: Jun-Seop
authors.initials: JS
authors.lastname: Shin
authors.affiliation: Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Xenotransplantation Research Centre (XRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Institute for Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea. Electronic address: yunilhee1212@gmail.com.
authors.forename: Il-Hee
authors.initials: IH
authors.lastname: Yoon
authors.affiliation: Xenotransplantation Research Centre (XRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Institute for Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea. Electronic address: vinaka00@chungbuk.ac.kr.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Xenotransplantation Research Centre (XRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea. Electronic address: tolerance@knu.ac.kr.
authors.forename: Yong-Hee
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Xenotransplantation Research Centre (XRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Institute for Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea. Electronic address: chgpark@snu.ac.kr.
authors.forename: Chung-Gyu
authors.initials: CG
authors.lastname: Park
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D004195:Disease Models, Animal; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL
keywords: Cecal vein; Intrahepatic; Islet; Transplantation