title: LC-MS-based quantification method for Achyranthes root saponins.
abstract: A liquid chromatography mass spectrometry (LC-MS) method was developed for simultaneous quantitative analysis of Achyranthes root saponins: chikusetsusaponins IVa (1) and V (2), achyranthosides B (3), C (4), D (5), E (6), and G (7), sulfachyranthosides B (8) and D (9), and betavulgarosides II (10) and IV (11). Satisfactory separation of the saponins was achieved with the use of a volatile ion-pair reagent (dihexyl ammonium acetate) on a phenyl-hexylated silica gel column, and the amounts of saponins extracted under three different conditions were determined. When Achyranthes root was extracted with water at room temperature, achyranthosides B (3) and D (5) were the major saponins, and smaller amounts of other saponins (4, 6-11) were present. However, the amounts of chikusetsusaponins (1 and 2) were negligible. Under the condition to make a standard decoction of a Kampo formula, the major saponins were achyranthosides B (3), C (4), and D (5), and small amounts of chikusetsusaponins IVa (1) and V (2) appeared, whereas prolonged heating largely increased the amounts of chikusetsusaponins. This method can be used for quality control of Achyranthes root.
authors.affiliation: Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo, 105-8512, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kawahara
authors.affiliation: Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo, 105-8512, Japan.
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Hoshino
authors.affiliation: Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo, 105-8512, Japan.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Morimoto
authors.affiliation: Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo, 105-8512, Japan.
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Shinizu
authors.affiliation: Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo, 105-8512, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Narukawa
authors.affiliation: Research Center for Medicinal Plant Resources, National Institute of Biomedical Innovation, Health and Nutrition, 1-2 Hachimandai, Tsukuba, Ibaragi, 305-0843, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fuchino
authors.affiliation: Research Center for Medicinal Plant Resources, National Institute of Biomedical Innovation, Health and Nutrition, 1-2 Hachimandai, Tsukuba, Ibaragi, 305-0843, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kawahara
authors.affiliation: Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo, 105-8512, Japan. kiuchi-fm@pha.keio.ac.jp.
authors.forename: Fumiyuki
authors.initials: F
authors.lastname: Kiuchi
mesh_terms: D029044:Achyranthes / Q000737:chemistry*; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D012503:Saponins / Q000032:analysis* / Q000737:chemistry
chemical_list: D012503:Saponins; C096070:achyranthoside C; C096071:achyranthoside D; C588229:chikusetsusaponin V; C098580:chikusetsu saponin IVa; D009828:Oleanolic Acid
keywords: Achyranthes bidentata; Achyranthoside; LC-MS; Oleanan saponin; Quantitative analysis; Sulfachyranthoside