title: ORF8-Related Genetic Evidence for Chinese Horseshoe Bats as the Source of Human Severe Acute Respiratory Syndrome Coronavirus.
abstract: Several lineage B betacoronaviruses termed severe acute respiratory syndrome (SARS)-like CoVs (SL-CoVs) were identified from Rhinolophus bats in China. These viruses are characterized by a set of unique accessory open reading frames (ORFs) that are located between the M and N genes. Among unique accessory ORFs, ORF8 is most hypervariable. In this study, the ORF8s of all SL-CoVs were classified into 3 types, and, for the first time, it was found that very few SL-CoVs from Rhinolophus sinicus have ORF8s that are identical to that of human SARS-CoV. This finding provides new genetic evidence for Chinese horseshoe bats as the source of human SARS-CoV.
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Wu
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.
authors.forename: Xianwen
authors.initials: X
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Estuarine and Coastal Research, Institute of Estuarine and Coastal Research, East China Normal University, Shanghai.
authors.forename: Junpeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, People's Republic of China.
authors.forename: Shuyi
authors.initials: S
authors.lastname: Zhang
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou.
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
mesh_terms: D000818:Animals; D002681:China; D002685:Chiroptera / Q000821:virology*; D016000:Cluster Analysis; D019143:Evolution, Molecular; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D045473:Severe acute respiratory syndrome-related coronavirus / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014763:Viral Matrix Proteins / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D014763:Viral Matrix Proteins; C488151:sars7a protein, SARS virus
keywords: ORF8; bats; origin; severe acute respiratory syndrome coronavirus