title: c-abl and YWHAZ gene expression in gastric cancer.
abstract: This study aims to determine the gene expression for c-abl and YWHAZ in gastric cancer and the differences between the c-abl and YWHAZ gene expression inside the tumor versus healthy tissue (at the resection edges). This prospective study included 34 patients with gastric neoplasia, 21 men and 13 women, aged between 49 and 79 years (65.5 years median). After the surgical procedure, in these cases, we collected two tissue samples: one sample was obtained from inside the tumoral tissue and another sample from the gastric tissue, which was identified as normal apparently, as far as possible from the tumor (resection edge). For determining the c-abl and YWHAZ gene expression, we used the quantitative real-time polymerase chain reaction. Regarding the c-abl gene expression in gastric cancer, c-abl expression was identified as lower inside tumor cells comparing to the normal gastric tissue (resection limit). This difference of gene expression emphasize the role of the c-abl gene in normal tissue growth and the involvement in apoptosis induction when alteration of DNA occurs, as a result to different agents actions as stress, ionizing radiations. The loss of expression or even the down-regulation of the c-abl is a fundamental event that leads to genesis and progression of tumors. No significant differences of the YWHAZ gene expression between the tumoral and normal gastric tissue probes were recorded in our study.
authors.affiliation: Second Surgical Clinic, University of Medicine and Pharmacy of Craiova, Romania; id.vilcea@yahoo.com.
authors.forename: Emil
authors.initials: E
authors.lastname: Moraru
authors.forename: Ionică Daniel
authors.initials: ID
authors.lastname: Vîlcea
authors.forename: Cecil Sorin
authors.initials: CS
authors.lastname: Mirea
authors.forename: Stelian Ştefăniţă
authors.initials: SŞ
authors.lastname: Mogoantă
authors.forename: Mihai
authors.initials: M
authors.lastname: Cruce
authors.forename: Ion
authors.initials: I
authors.lastname: Vasile
authors.forename: Manuela
authors.initials: M
authors.lastname: Vasile
authors.forename: Alina Maria
authors.initials: AM
authors.lastname: Vîlcea
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism*; D000368:Aged; D017209:Apoptosis; D004247:DNA / Q000032:analysis; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D016315:Proto-Oncogene Proteins c-abl / Q000378:metabolism*; D011839:Radiation, Ionizing; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000601:surgery
chemical_list: D048948:14-3-3 Proteins; C572235:YWHAZ protein, human; D004247:DNA; D016315:Proto-Oncogene Proteins c-abl