title: Mechanobioreactors for Cartilage Tissue Engineering.
abstract: Mechanical stimulation is an effective method to increase extracellular matrix synthesis and to improve the mechanical properties of tissue-engineered cartilage constructs. In this chapter, we describe valuable methods of imposing direct mechanical stimuli (compression or shear) to tissue-engineered cartilage constructs as well as some common analytical methods used to quantify the effects of mechanical stimuli after short-term or long-term loading.
authors.affiliation: Department of Mechanical & Materials Engineering, Queen's University, Kingston, Canada. weberj@me.queensu.ca.|Human Mobility Research Centre, Kingston General Hospital and Queen's University, Kingston, Canada. weberj@me.queensu.ca.|Kennan Research Centre, Li Ka Shing Knowledge Institute, St. Michael's Hospital, 30 Bond Street, Toronto, Canada, M5B 1W8. weberj@me.queensu.ca.
authors.forename: Joanna F
authors.initials: JF
authors.lastname: Weber
authors.affiliation: Kennan Research Centre, Li Ka Shing Knowledge Institute, St. Michael's Hospital, 30 Bond Street, Toronto, Canada, M5B 1W8. roman.perez@upc.edu.|Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea. roman.perez@upc.edu.
authors.forename: Roman
authors.initials: R
authors.lastname: Perez
authors.affiliation: Department of Mechanical & Materials Engineering, Queen's University, Kingston, Canada. swaldman@ryerson.ca.|Kennan Research Centre, Li Ka Shing Knowledge Institute, St. Michael's Hospital, 30 Bond Street, Toronto, Canada, M5B 1W8. swaldman@ryerson.ca.|Department of Chemical Engineering, Ryerson University, Kerr Hall South (KHS 241), 50 Gould Street, Toronto, ON, Canada, M5B 2K3. swaldman@ryerson.ca.
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Waldman
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D019149:Bioreactors; D002358:Cartilage, Articular / Q000166:cytology* / Q000378:metabolism / Q000601:surgery; D002417:Cattle; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D020219:Chondrogenesis; D004867:Equipment Design; D020100:Hydrogels; D040542:Mechanotransduction, Cellular; D010812:Physical Stimulation; D044968:Regenerative Medicine / Q000295:instrumentation* / Q000379:methods; D012685:Sepharose / Q000737:chemistry; D013314:Stress, Mechanical; D013997:Time Factors; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D020100:Hydrogels; D012685:Sepharose
keywords: Cartilage; Chondrocytes; Collagen; Mechanical stimulation; Mechanotransduction; Proteoglycans; Tissue engineering