title: Paenibacillus ripae sp. nov., isolated from bank side soil.
abstract: A Gram-stain-variable, rod-shaped, non-motile and endospore-forming bacterium, designated strain HZ1T, was isolated from a sample of bank side soil from Hangzhou city, Zhejiang province, PR China. On the basis of 16S rRNA gene sequence analysis, strain HZ1T was closely related to members of the genus Paenibacillus, sharing the highest levels of sequence similarity with Paenibacillus agarexedens DSM 1327T (94.4 %), Paenibacillus sputi KIT00200-70066-1T (94.4 %). Growth occurred at 15-42 °C (optimum 30-37 °C), pH 5.0-9.5 (optimum pH 7.0-8.0) and NaCl concentrations of up to 6.0 % (w/v) were tolerated (optimum 0.5 %). The dominant respiratory quinone was MK-7 and the DNA G+C content was 40.1 mol%. The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0. The major polar lipids of strain HZ1T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and several unknown lipids. The diagnostic diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. Based on its phenotypic and chemotaxonomic characteristics and phylogenetic data, strain HZ1T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus ripae sp. nov. (type strain HZ1T = CCTCC AB 2014276T = LMG 28639T) is proposed.
authors.affiliation: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Institute of Microbiology, School of Life Science, Lanzhou University, Tianshui Road No. 222, Lanzhou 730000, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Gansu Institute for Drug Control, Yinan Road No. 7, Lanzhou 730070, PR China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, School of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Institute of Microbiology, School of Life Science, Lanzhou University, Tianshui Road No. 222, Lanzhou 730000, PR China.
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Institute of Microbiology, School of Life Science, Lanzhou University, Tianshui Road No. 222, Lanzhou 730000, PR China.|Gansu Corps Hospital of CAPF, Gansu 730000, PR China.
authors.forename: QinJian
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Institute of Microbiology, School of Life Science, Lanzhou University, Tianshui Road No. 222, Lanzhou 730000, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Institute of Microbiology, School of Life Science, Lanzhou University, Tianshui Road No. 222, Lanzhou 730000, PR China.
authors.forename: Ronghui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Institute for Microbial and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Donggang Road No. 199, Lanzhou 730020, PR China.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Institute for Microbial and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Donggang Road No. 199, Lanzhou 730020, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: Institute for Microbial and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Donggang Road No. 199, Lanzhou 730020, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute for Microbial and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Donggang Road No. 199, Lanzhou 730020, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute for Microbial and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Donggang Road No. 199, Lanzhou 730020, PR China.|Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Institute of Microbiology, School of Life Science, Lanzhou University, Tianshui Road No. 222, Lanzhou 730000, PR China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7