title: A New Adjuvant Combined with Inactivated Influenza Enhances Specific CD8 T Cell Response in Mice and Decreases Symptoms in Swine Upon Challenge.
abstract: Vaccination is the most effective way to control swine influenza virus (SIV) in the field. Classical vaccines are based on inactivated antigens formulated with an oil emulsion or a polymeric adjuvant. Standard adjuvants enhance the humoral response and orient the immune response toward a Th2 response. An important issue is that current vaccines do not protect against new strains. One approach to improve cross-protection is to enhance Th1 and cytotoxic responses. The development of adjuvants orienting the immune response of inactivated vaccines toward Th1/Cytotoxic responses would be highly beneficial. This study shows that the water in oil in water emulsion adjuvant Montanide™ ISA 201 VG allows the induction of anti-influenza CD8 T cell in mice and induces homologous protection against an H1N1 challenge in swine. Such adjuvants that induce both humoral and cell-mediated immunity could improve the protection conferred by SIV vaccines in the field.
authors.affiliation: 1 Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique , Domaine de Vilvert, Jouy-en-Josas, France .
authors.forename: Edwige
authors.initials: E
authors.lastname: Bouguyon
authors.affiliation: 2 SEPPIC , Paris La Défense, France .
authors.forename: Elodie
authors.initials: E
authors.lastname: Goncalves
authors.affiliation: 3 FGBI "Federal Centre for Animal Health" (FGBI "ARRIAH") , Yur'evets, Vladimir, Russia .
authors.forename: Alexander
authors.initials: A
authors.lastname: Shevtsov
authors.affiliation: 1 Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique , Domaine de Vilvert, Jouy-en-Josas, France .
authors.forename: Pauline
authors.initials: P
authors.lastname: Maisonnasse
authors.affiliation: 3 FGBI "Federal Centre for Animal Health" (FGBI "ARRIAH") , Yur'evets, Vladimir, Russia .
authors.forename: Stepan
authors.initials: S
authors.lastname: Remyga
authors.affiliation: 3 FGBI "Federal Centre for Animal Health" (FGBI "ARRIAH") , Yur'evets, Vladimir, Russia .
authors.forename: Oleg
authors.initials: O
authors.lastname: Goryushev
authors.affiliation: 2 SEPPIC , Paris La Défense, France .
authors.forename: Sebastien
authors.initials: S
authors.lastname: Deville
authors.affiliation: 1 Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique , Domaine de Vilvert, Jouy-en-Josas, France .
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bertho
authors.affiliation: 2 SEPPIC , Paris La Défense, France .
authors.forename: Juliette
authors.initials: J
authors.lastname: Ben Arous
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology; D007252:Influenza Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D008810:Mice, Inbred C57BL; D009976:Orthomyxoviridae Infections / Q000473:pathology* / Q000517:prevention & control*; D013552:Swine; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated