title: Analysis of Amphetamine-Derived Designer Drugs by Gas Chromatography with Mass Spectrometry.
abstract: The 22 amphetamine-derived synthetic drugs (ADSDs), mostly cathinones, were examined by gas chromatography with mass spectrometry using two different derivatization methods with (i) heptafluorobutyric anhydride (HFBA) and (ii) pentafluorobenzoyl chloride (PFBCl). Both developed derivatization approaches were evaluated and compared for urine and serum samples. Extraction procedures proved to give satisfactory results with regard to recoveries and extract purity, even though both derivatization methods reached acceptable sensitivity for the intended use. The derivatization with PFBCl showed better results with respect to retention and response stability, thus the PFBCl method was selected for validation. Calibration curves were linear over the tested concentration range of 20-1,000 ng/mL with the R(2) values ranging from 0.994 to 0.998. Intra- and interday precisions and accuracies were within 20% for all concentrations in the linear range. The limit of detection was determined to be lower than 2 ng/mL for all 22 analytes. The method proved to be a useful analytical tool in the course of systematic toxicological analysis.
authors.affiliation: Faculty of Medicine and Dentistry, Department of Forensic Medicine and Medical Law, Palacký University, Olomouc CZ-77509, Czech Republic Faculty Hospital, Palacký University, Hnevotinska 3, Olomouc CZ-77509, Czech Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Ondra
authors.affiliation: Faculty of Medicine and Dentistry, Department of Forensic Medicine and Medical Law, Palacký University, Olomouc CZ-77509, Czech Republic Faculty Hospital, Palacký University, Hnevotinska 3, Olomouc CZ-77509, Czech Republic.
authors.forename: Ivo
authors.initials: I
authors.lastname: Válka
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Faculty of Science, Department of Analytical Chemistry, Palacký University, 17 Listopadu 12, Olomouc CZ-77146, Czech Republic.
authors.forename: Radim
authors.initials: R
authors.lastname: Knob
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Faculty of Science, Department of Analytical Chemistry, Palacký University, 17 Listopadu 12, Olomouc CZ-77146, Czech Republic.
authors.forename: Pavlína
authors.initials: P
authors.lastname: Ginterová
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Faculty of Science, Department of Analytical Chemistry, Palacký University, 17 Listopadu 12, Olomouc CZ-77146, Czech Republic vitezslav.maier@upol.cz.
authors.forename: Vítězslav
authors.initials: V
authors.lastname: Maier
mesh_terms: D000662:Amphetamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D053000:Analytic Sample Preparation Methods; D001565:Benzoates / Q000737:chemistry; D002138:Calibration; D015198:Designer Drugs / Q000032:analysis*; D005466:Fluorocarbons / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D057230:Limit of Detection; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000379:methods*; D005464:Fluorobenzenes
chemical_list: D000662:Amphetamines; D001565:Benzoates; D015198:Designer Drugs; D005466:Fluorocarbons; C014737:pentafluorobenzoyl chloride; D005464:Fluorobenzenes; C007924:heptafluorobutyric anhydride