title: Aptamer Recognition Induced Target-Bridged Strategy for Proteins Detection Based on Magnetic Chitosan and Silver/Chitosan Nanoparticles Using Surface-Enhanced Raman Spectroscopy.
abstract: Poor selectivity and biocompability remain problems in applying surface-enhanced Raman spectroscopy (SERS) for direct detection of proteins due to similar spectra of most proteins and overlapping Raman bands in complex mixtures. To solve these problems, an aptamer recognition induced target-bridged strategy based on magnetic chitosan (MCS) and silver/chitosan nanoparticles (Ag@CS NPs) using SERS was developed for detection of protein benefiting from specific affinity of aptamers and biocompatibility of chitosan (CS). In this process, one aptamer (or antibody) modified MCS worked as capture probes through the affinity binding site of protein. The other aptamer modified Raman report molecules encapsulated Ag@CS NPs were used as SERS sensing probes based on the other binding site of protein. The sandwich complexes of aptamer (antibody)/protein/aptamer were separated easily with a magnet from biological samples, and the concentration of protein was indirectly reflected by the intensity variation of SERS signal of Raman report molecules. To explore the universality of the strategy, three different kinds of proteins including thrombin, platelet derived growth factor BB (PDGF BB) and immunoglobulin E (lgE) were investigated. The major advantages of this aptamer recognition induced target-bridged strategy are convenient operation with a magnet, stable signal expressing resulting from preventing loss of report molecules with the help of CS shell, and the avoidance of slow diffusion-limited kinetics problems occurring on a solid substrate. To demonstrate the feasibility of the proposed strategy, the method was applied to detection of PDGF BB in clinical samples. The limit of detection (LOD) of PDGF BB was estimated to be 3.2 pg/mL. The results obtained from human serum of healthy persons and cancer patients using the proposed strategy showed good agreement with that of the ELISA method but with wider linear range, more convenient operation, and lower cost. The proposed strategy holds great potential in highly sensitive and selective analysis of target proteins in complex biological samples.
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University , Guangzhou 510275, China.
authors.forename: Jincan
authors.initials: J
authors.lastname: He
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University , Guangzhou 510275, China.
authors.forename: Gongke
authors.initials: G
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University , Guangzhou 510275, China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Hu
mesh_terms: D052158:Aptamers, Peptide / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D057230:Limit of Detection; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D052158:Aptamers, Peptide; D011506:Proteins; D012834:Silver; D048271:Chitosan