title: [A260G and A386G single nucleotide polymorphisms of the DAZL gene are not correlated with male infertility in the Chinese population of Zhejiang Province].
abstract: OBJECTIVE
  To assess the association of the A260G and A386G single nucleotide polymorphisms (SNP) of the DAZL gene with male infertility in the Chinese population of Zhejiang Province.
  
  
  METHODS
  We collected the peripheral blood samples from 317 idiopathic infertile males with azoospermia or oligozoospermia and 246 normal fertile men, and genotyped the polymorphic loci of the A260G and A386G polymorphisms of the DAZL gene using the SNaPshot technique.
  
  
  RESULTS
  The DAZL gene A260G was found genetically polymorphic in the Chinese population of Zhejiang Province, with the gene frequencies and their distribution consistent to the Hardy-Weinberg equilibrium. The frequencies of the AA, AG and GG genotypes of the A260G polymorphism were 92.3%, 7.3%, and 0.4% respectively in the normal controls and 94.3%, 5.7%, and 0% in the infertile patients, with no statistically significant differences between the two groups (P = 0.43, OR = 0.78, 95% CI 0.413-1.46). Heterozygosis (AG) of A386G was found in 1 of the control males but not in the infertile patients, while homozygosis (GG) of A386G was not observed in either group (P = 0.259, OR = 0.698, 59% CI: 0.374-1.306).
  
  
  CONCLUSIONS
  A260G and A386G SNPs of the DAZL gene are not associated with spermatogenic failure and neither represents a molecular marker for the genetic diagnosis of male infertility in the Chinese population of Zhejiang Province.
authors.forename: Wu-hua
authors.initials: WH
authors.lastname: Ni
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Yang
authors.forename: Qian-jin
authors.initials: QJ
authors.lastname: Fei
authors.forename: Cheng-shuang
authors.initials: CS
authors.lastname: Pan
authors.forename: Xue-feng
authors.initials: XF
authors.lastname: Huang
mesh_terms: D044466:Asian People; D053713:Azoospermia / Q000235:genetics; D002681:China; D005787:Gene Frequency; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D009845:Oligospermia / Q000235:genetics; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D016601:RNA-Binding Proteins / Q000235:genetics*
chemical_list: C101358:DAZL protein, human; D005819:Genetic Markers; D016601:RNA-Binding Proteins