title: Production of Immunoabsorbent Nanoparticles by Displaying Single-Domain Protein A on Potato Virus X.
abstract: The combination of antibodies with nanoparticles provides wide-ranging applications in biosensing. While several covalent presentation strategies have been established, there is need for alternative, non-covalent methods to provide a routine for scalable nanomanufacturing. We report the multivalent presentation of the B domain of Staphylococcus aureus protein A (SpAB) on potato virus X (PVX) nanoparticles. Three different synthetic strategies were used to obtain chimeric PVX(SpAB) filaments. The protein A fragments displayed on the surface of all three PVX chimeras remained fully functional as an immunoabsorbent for antibody capture enabling biosensing. The new biomaterials presented could find applications as diagnostic tools for biomedical or environmental monitoring.
authors.affiliation: Institute for Molecular Biotechnology, RWTH Aachen University, Worringer Weg 1, 52074 Aachen, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Uhde-Holzem
authors.affiliation: Department of Biomedical Engineering, Case Western Reserve University Schools of Medicine and Engineering, Cleveland, Ohio 44106, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: McBurney
authors.affiliation: Department of Macromolecular Science and Engineering, Case Western Reserve University Schools of Medicine and Engineering, Cleveland, Ohio 44106, USA.
authors.forename: Brylee David B
authors.initials: BD
authors.lastname: Tiu
authors.affiliation: Department of Macromolecular Science and Engineering, Case Western Reserve University Schools of Medicine and Engineering, Cleveland, Ohio 44106, USA.
authors.forename: Rigoberto C
authors.initials: RC
authors.lastname: Advincula
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6, 52074, Aachen, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Fischer
authors.affiliation: Institute for Molecular Biotechnology, RWTH Aachen University, Worringer Weg 1, 52074 Aachen, Germany. commandeur@molbiotech.rwth-aachen.de.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Commandeur
authors.affiliation: Department of Biomedical Engineering, Case Western Reserve University Schools of Medicine and Engineering, Cleveland, Ohio 44106, USA. nicole.steinmetz@case.edu.|Department of Macromolecular Science and Engineering, Case Western Reserve University Schools of Medicine and Engineering, Cleveland, Ohio 44106, USA. nicole.steinmetz@case.edu.|Department of Radiology, Case Western Reserve University Schools of Medicine and Engineering, Cleveland, Ohio 44106, USA. nicole.steinmetz@case.edu.|Department of Materials Science and Engineering, Case Western Reserve University Schools of Medicine and Engineering, Cleveland, Ohio 44106, USA. nicole.steinmetz@case.edu.
authors.forename: Nicole F
authors.initials: NF
authors.lastname: Steinmetz
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D017863:Potexvirus / Q000737:chemistry* / Q000235:genetics; D013205:Staphylococcal Protein A / Q000737:chemistry* / Q000235:genetics; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000235:genetics
chemical_list: D013205:Staphylococcal Protein A