title: Short-Chain Fatty Acids Regulate Secretion of IL-8 from Human Intestinal Epithelial Cell Lines in vitro.
abstract: Short-chain fatty acids (SCFAs) including acetate, propionate and butyrate play an important role in the physiological functions of epithelial cells and colonocytes, such as immune response regulation. Human intestinal epithelial cells (IECs) contribute in intestinal immune response via different ways, such as production of different immune factors including Interleukin (IL) IL-8, which act as chemoattractant for neutrophils, and subsequently enhance inflammation. Therefore, we aimed to evaluate the effects of SCFAs on IECs viability and production of IL-8 in vitro. SCFAs were co-cultured with either normal intestinal epithelial (T4056) or adenocarcinoma derived (HT-29) cell lines for 24-96 h in the presence of E.coli lipopolysaccharides (LPS). Cell viability, proliferation, production of IL-8 and expression of IL-8 mRNA were determined in the cell cultures. The result showed that 20 mM of SCFAs was non-cytotoxic to T4056 and enhanced their growth, whereas the growth of HT-29 was inhibited. The SCFAs down regulated LPS-stimulated IL-8 secretion with different response patterns, but no obvious effects on the release of IL-8 from non LPS- stimulated cells. In conclusion, SCFAs showed regulatory effect on release of LPS-stimulated IL-8 as well as the expression of mRNA of IL-8; these might explain the anti-inflammatory and anti-carcinogenic mechanism of SCFAs.
authors.affiliation: a Advanced Food Systems Research Unit, College of Health and Biomedicine, Victoria University , Melbourne , Victoria , Australia .|b Faculty of Veterinary Medicine , Tripoli University , Tripoli , Libya , and.
authors.forename: M
authors.initials: M
authors.lastname: Asarat
authors.affiliation: a Advanced Food Systems Research Unit, College of Health and Biomedicine, Victoria University , Melbourne , Victoria , Australia .
authors.forename: T
authors.initials: T
authors.lastname: Vasiljevic
authors.affiliation: c College of Health and Biomedicine, Centre for Chronic Disease Prevention and Management, Victoria University , Melbourne , Victoria , Australia.
authors.forename: V
authors.initials: V
authors.lastname: Apostolopoulos
authors.affiliation: a Advanced Food Systems Research Unit, College of Health and Biomedicine, Victoria University , Melbourne , Victoria , Australia .
authors.forename: O
authors.initials: O
authors.lastname: Donkor
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000378:metabolism* / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D005232:Fatty Acids, Volatile; D016209:Interleukin-8; D012333:RNA, Messenger
keywords: HT-29 cell line; T4056 cell line; interleukin-8; intestinal epithelial cells; short-chain fatty acids