title: Mitochondrion-Specific Live-Cell Bioprobe Operated in a Fluorescence Turn-On Manner and a Well-Designed Photoactivatable Mechanism.
abstract: A novel mitochondrion-specific live-cell fluorescence turn-on photoactivatable bioprobe, named o-TPP3M, is designed and readily prepared, operating in a new photoactivatable mechanism based on a twisted intramolecular charge-transfer effect, the restriction of intramolecular rotations, and photocyclodehydrogenation. This bioprobe exhibits unique photoactivation behavior, a large signal-to-background ratio, and no cytotoxicity to living cells, providing a promising candidate for varied bioresearch.
authors.affiliation: HKUST-Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area, Hi-Tech Park, Nanshan, Shenzhen, 518057, China.|Departments of Chemistry, Physics, and Division of Life Science, Institute of Molecular Functional Materials and Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Xinggui
authors.initials: X
authors.lastname: Gu
authors.affiliation: HKUST-Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area, Hi-Tech Park, Nanshan, Shenzhen, 518057, China.|Departments of Chemistry, Physics, and Division of Life Science, Institute of Molecular Functional Materials and Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Engui
authors.initials: E
authors.lastname: Zhao
authors.affiliation: HKUST-Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area, Hi-Tech Park, Nanshan, Shenzhen, 518057, China.|Departments of Chemistry, Physics, and Division of Life Science, Institute of Molecular Functional Materials and Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Jacky W Y
authors.initials: JW
authors.lastname: Lam
authors.affiliation: Key Laboratory of Organic Solids, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Peng
authors.affiliation: Key Laboratory of Organic Solids, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Departments of Chemistry, Physics, and Division of Life Science, Institute of Molecular Functional Materials and Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Departments of Chemistry, Physics, and Division of Life Science, Institute of Molecular Functional Materials and Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Kam Sing
authors.initials: KS
authors.lastname: Wong
authors.affiliation: Departments of Chemistry, Physics, and Division of Life Science, Institute of Molecular Functional Materials and Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Herman H Y
authors.initials: HH
authors.lastname: Sung
authors.affiliation: Departments of Chemistry, Physics, and Division of Life Science, Institute of Molecular Functional Materials and Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Ian D
authors.initials: ID
authors.lastname: Williams
authors.affiliation: HKUST-Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area, Hi-Tech Park, Nanshan, Shenzhen, 518057, China.|Departments of Chemistry, Physics, and Division of Life Science, Institute of Molecular Functional Materials and Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.|Guangdong Innovative Research Team, SCUT-HKUST Joint Research Laboratory, State Key Laboratory of Luminescent Materials and Device, South China University of Technology, Guangzhou, 51640, China.
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008027:Light; D008928:Mitochondria / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation
chemical_list: D005456:Fluorescent Dyes
keywords: bioprobes; mitochondrion-specific; photoactivatable fluorophores; photocyclodehydrogenation; selective live-cell imaging