title: Protein repair L-isoaspartyl methyltransferase 1 (PIMT1) in rice improves seed longevity by preserving embryo vigor and viability.
abstract: Damaged proteins containing abnormal isoaspartyl (isoAsp) accumulate as seeds age and the abnormality is thought to undermine seed vigor. Protein-L-isoaspartyl methyltransferase (PIMT) is involved in isoAsp-containing protein repair. Two PIMT genes from rice (Oryza sativa L.), designated as OsPIMT1 and OsPIMT2, were isolated and investigated for their roles. The results indicated that OsPIMT2 was mainly present in green tissues, but OsPIMT1 largely accumulated in embryos. Confocal visualization of the transient expression of OsPIMTs showed that OsPIMT2 was localized in the chloroplast and nucleus, whereas OsPIMT1 was predominately found in the cytosol. Artificial aging results highlighted the sensitivity of the seeds of OsPIMT1 mutant line when subjected to accelerated aging. Overexpression of OsPIMT1 in transgenic seeds reduced the accumulation of isoAsp-containing protein in embryos, and increased embryo viability. The germination percentage of transgenic seeds overexpressing OsPIMT1 increased 9-15% compared to the WT seeds after 21-day of artificial aging, whereas seeds from the OsPIMT1 RNAi lines overaccumulated isoAsp in embryos and experienced rapid loss of seed germinability. Taken together, these data strongly indicated that OsPIMT1-related seed longevity improvement is probably due to the repair of detrimental isoAsp-containing proteins that over accumulate in embryos when subjected to accelerated aging.
authors.affiliation: Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China.|Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fuzhou, Fujian, China.|Incubator of National Key Laboratory of Crop Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture/South-China Base of National Key Laboratory of Hybrid Rice of China, Fuzhou/National Engineering Laboratory of Rice, Fuzhou, Fujian, China.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China.|Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fuzhou, Fujian, China.|Incubator of National Key Laboratory of Crop Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture/South-China Base of National Key Laboratory of Hybrid Rice of China, Fuzhou/National Engineering Laboratory of Rice, Fuzhou, Fujian, China.
authors.forename: Huibin
authors.initials: H
authors.lastname: Xu
authors.affiliation: Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China.|Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fuzhou, Fujian, China.|Incubator of National Key Laboratory of Crop Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture/South-China Base of National Key Laboratory of Hybrid Rice of China, Fuzhou/National Engineering Laboratory of Rice, Fuzhou, Fujian, China.|Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Diao
authors.affiliation: Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China.|Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fuzhou, Fujian, China.|Incubator of National Key Laboratory of Crop Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture/South-China Base of National Key Laboratory of Hybrid Rice of China, Fuzhou/National Engineering Laboratory of Rice, Fuzhou, Fujian, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China.|Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fuzhou, Fujian, China.|Incubator of National Key Laboratory of Crop Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture/South-China Base of National Key Laboratory of Hybrid Rice of China, Fuzhou/National Engineering Laboratory of Rice, Fuzhou, Fujian, China.
authors.forename: Hongguang
authors.initials: H
authors.lastname: Xie
authors.affiliation: Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China.|Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fuzhou, Fujian, China.|Incubator of National Key Laboratory of Crop Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture/South-China Base of National Key Laboratory of Hybrid Rice of China, Fuzhou/National Engineering Laboratory of Rice, Fuzhou, Fujian, China.
authors.forename: Qiuhua
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China.|Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fuzhou, Fujian, China.|Incubator of National Key Laboratory of Crop Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture/South-China Base of National Key Laboratory of Hybrid Rice of China, Fuzhou/National Engineering Laboratory of Rice, Fuzhou, Fujian, China.
authors.forename: Fangxi
authors.initials: F
authors.lastname: Wu
authors.affiliation: Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fuzhou, Fujian, China.|Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Zonghua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China. jianfzhang@163.com.|Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fuzhou, Fujian, China. jianfzhang@163.com.|Incubator of National Key Laboratory of Crop Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture/South-China Base of National Key Laboratory of Hybrid Rice of China, Fuzhou/National Engineering Laboratory of Rice, Fuzhou, Fujian, China. jianfzhang@163.com.
authors.forename: Jianfu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China. huaanxie@163.com.|Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fuzhou, Fujian, China. huaanxie@163.com.|Incubator of National Key Laboratory of Crop Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture/South-China Base of National Key Laboratory of Hybrid Rice of China, Fuzhou/National Engineering Laboratory of Rice, Fuzhou, Fujian, China. huaanxie@163.com.
authors.forename: Huaan
authors.initials: H
authors.lastname: Xie
mesh_terms: D000040:Abscisic Acid / Q000096:biosynthesis; D000595:Amino Acid Sequence; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000196:embryology / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D026601:Protein D-Aspartate-L-Isoaspartate Methyltransferase / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D050436:Regulatory Elements, Transcriptional; D012639:Seeds / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D000040:Abscisic Acid; D026601:Protein D-Aspartate-L-Isoaspartate Methyltransferase
keywords: Embryo viability; Embryo vigor; Protein repair L-isoaspartyl methyltransferase 1 (PIMT1); Seed longevity