title: Establishment and validation of a 384-well antibacterial assay amenable for high-throughput screening and combination testing.
abstract: A 384-well-based antibacterial assay amenable for high-throughput screening and combination testing is described. The assay uses 100-500nL of test compounds and tolerates up to 2.5% dimethyl sulfoxide concentrations. It can be used for screening compound libraries and testing combinatory/synergistic/antagonistic effects of antibiotics, small molecules, and natural product extracts.
authors.affiliation: Biology and Pharmacology, Albany Molecular Research, Inc., The Galen, #05-01, 61 Science Park Road, 117525, Singapore.
authors.forename: Arti
authors.initials: A
authors.lastname: Mishra
authors.affiliation: Biology and Pharmacology, Albany Molecular Research, Inc., The Galen, #05-01, 61 Science Park Road, 117525, Singapore; Lead Discovery, Albany Molecular Research, Inc., 22215 26th Avenue SE, Bothell, WA 98021, United States.
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Dobritsa
authors.affiliation: Lead Discovery, Albany Molecular Research, Inc., 22215 26th Avenue SE, Bothell, WA 98021, United States.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Crouch
authors.affiliation: Lead Discovery, Albany Molecular Research, Inc., 22215 26th Avenue SE, Bothell, WA 98021, United States.
authors.forename: John
authors.initials: J
authors.lastname: Rabenstein
authors.affiliation: Biology and Pharmacology, Albany Molecular Research, Inc., The Galen, #05-01, 61 Science Park Road, 117525, Singapore.
authors.forename: Joycelyn Xiang Yi
authors.initials: JX
authors.lastname: Lee
authors.affiliation: Biology and Pharmacology, Albany Molecular Research, Inc., The Galen, #05-01, 61 Science Park Road, 117525, Singapore. Electronic address: Saravanakumar.Dhakshinamoorthy@amriglobal.com.
authors.forename: Saravanakumar
authors.initials: S
authors.lastname: Dhakshinamoorthy
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004347:Drug Interactions; D057166:High-Throughput Screening Assays; D008826:Microbial Sensitivity Tests / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibacterial assay; Combination testing; DMSO tolerance; HTS