title: Influence of Factors of Cryopreservation and Hypothermic Storage on Survival and Functional Parameters of Multipotent Stromal Cells of Placental Origin.
abstract: Human placenta is a highly perspective source of multipotent stromal cells (MSCs) both for the purposes of patient specific auto-banking and allogeneic application in regenerative medicine. Implementation of new GMP standards into clinical practice enforces the search for relevant methods of cryopreservation and short-term hypothermic storage of placental MSCs. In this paper we analyze the effect of different temperature regimes and individual components of cryoprotective media on viability, metabolic and culture properties of placental MSCs. We demonstrate (I) the possibility of short-term hypothermic storage of these cells; (II) determine DMSO and propanediol as the most appropriate cryoprotective agents; (III) show the possibility of application of volume expanders (plasma substituting solutions based on dextran or polyvinylpyrrolidone); (IV) reveal the priority of ionic composition over the serum content in cryopreservation media; (V) determine a cooling rate of 1°C/min down to -40°C followed by immersion into liquid nitrogen as the optimal cryopreservation regime for this type of cells. This study demonstrates perspectives for creation of new defined cryopreservation methods towards GMP standards.
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkiv, Ukraine; Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany.
authors.forename: Denys
authors.initials: D
authors.lastname: Pogozhykh
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkiv, Ukraine.
authors.forename: Volodymyr
authors.initials: V
authors.lastname: Prokopyuk
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkiv, Ukraine; Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany.
authors.forename: Olena
authors.initials: O
authors.lastname: Pogozhykh
authors.affiliation: Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Mueller
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkiv, Ukraine.
authors.forename: Olga
authors.initials: O
authors.lastname: Prokopyuk
mesh_terms: D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D006801:Humans; D039902:Multipotent Stem Cells / Q000187:drug effects*; D010920:Placenta / Q000187:drug effects*; D011247:Pregnancy
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide