title: Rapid assay of stem cell functionality and potency using electric cell-substrate impedance sensing.
abstract: Regenerative medicine studies using autologous bone marrow mononuclear cells (BM-MNCs) have shown improved clinical outcomes that correlate to in vitro BM-MNC invasive capacity. The current Boyden-chamber assay for testing invasive capacity is labor-intensive, provides only a single time point, and takes 36 hours to collect data and results, which is not practical from a clinical cell delivery perspective. To develop a rapid, sensitive and reproducible invasion assay, we employed Electric Cell-substrate Impedance Sensing (ECIS) technology. Chemokine-directed BM-MNC cell invasion across a Matrigel-coated Transwell filter was measurable within minutes using the ECIS system we developed. This ECIS-Transwell chamber system provides a rapid and sensitive test of stem and progenitor cell invasive capacity for evaluation of stem cell functionality to provide timely clinical data for selection of patients likely to realize clinical benefit in regenerative medicine treatments. This device could also supply robust unambiguous, reproducible and cost effective data as a potency assay for cell product release and regulatory strategies.
authors.affiliation: Center for Regenerative Medicine, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, 97239, Portland, OR, USA. ruttenm@ohsu.edu.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Rutten
authors.affiliation: Center for Regenerative Medicine, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, 97239, Portland, OR, USA. larawayb@ohsu.edu.
authors.forename: Bryan
authors.initials: B
authors.lastname: Laraway
authors.affiliation: Center for Regenerative Medicine, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, 97239, Portland, OR, USA. gregoryc@ohsu.edu.|VA Portland Health Care System, 3710 SW US Veterans Hospital Road, 97239, Portland, OR, USA. gregoryc@ohsu.edu.|Department of Molecular Microbiology and Immunology, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, 97239, Portland, OR, USA. gregoryc@ohsu.edu.
authors.forename: Cynthia R
authors.initials: CR
authors.lastname: Gregory
authors.affiliation: Center for Regenerative Medicine, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, 97239, Portland, OR, USA. xieh@ohsu.edu.
authors.forename: Hua
authors.initials: H
authors.lastname: Xie
authors.affiliation: Applied BioPhysics, Inc., 185 Jordan Road, 12180, Troy, NY, USA. renken@biophysics.com.
authors.forename: Christian
authors.initials: C
authors.lastname: Renken
authors.affiliation: Applied BioPhysics, Inc., 185 Jordan Road, 12180, Troy, NY, USA. keese@biophysics.com.
authors.forename: Charles
authors.initials: C
authors.lastname: Keese
authors.affiliation: Center for Regenerative Medicine, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, 97239, Portland, OR, USA. gregoryk@ohsu.edu.|Department of Biomedical Engineering, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, 97239, Portland, OR, USA. gregoryk@ohsu.edu.
authors.forename: Kenton W
authors.initials: KW
authors.lastname: Gregory
mesh_terms: D000818:Animals; D002465:Cell Movement; D017097:Electric Impedance; D006801:Humans; D019169:Jurkat Cells; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D008297:Male; D013234:Stem Cells / Q000502:physiology*; D013552:Swine; D013556:Swine, Miniature