title: Analysis of Chuanxiong Rhizoma and its active components by Fourier transform infrared spectroscopy combined with two-dimensional correlation infrared spectroscopy.
abstract: As complicated mixture systems, active components of Chuanxiong Rhizoma are very difficult to identify and discriminate. In this paper, the macroscopic IR fingerprint method including Fourier transform infrared spectroscopy (FT-IR), the second derivative infrared spectroscopy (SD-IR) and two-dimensional correlation infrared spectroscopy (2DCOS-IR), was applied to study and identify Chuanxiong raw materials and its different segmented production of HPD-100 macroporous resin. Chuanxiong Rhizoma is rich in sucrose. In the FT-IR spectra, water eluate is more similar to sucrose than the powder and the decoction. Their second derivative spectra amplified the differences and revealed the potentially characteristic IR absorption bands and combined with the correlation coefficient, concluding that 50% ethanol eluate had more ligustilide than other eluates. Finally, it can be found from 2DCOS-IR spectra that proteins were extracted by ethanol from Chuanxiong decoction by HPD-100 macroporous resin. It was demonstrated that the above three-step infrared spectroscopy could be applicable for quick, non-destructive and effective analysis and identification of very complicated and similar mixture systems of traditional Chinese medicines.
authors.affiliation: School of Chinese Pharmacology, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Guo
authors.affiliation: School of Chinese Pharmacology, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Beiran
authors.initials: B
authors.lastname: Lv
authors.affiliation: School of Chinese Pharmacology, Beijing University of Chinese Medicine, Beijing 100102, China. Electronic address: jingjuanw@163.com.
authors.forename: Jingjuan
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Chinese Pharmacology, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Tsinghua University, Beijing 100084, China. Electronic address: sunsq@mail.tsinghua.edu.cn.
authors.forename: Suqin
authors.initials: S
authors.lastname: Sun
authors.affiliation: School of Chinese Pharmacology, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: School of Chinese Pharmacology, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Lu
authors.affiliation: School of Chinese Pharmacology, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiang
authors.affiliation: School of Chinese Pharmacology, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: School of Chinese Pharmacology, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qu
authors.affiliation: Haerbin 242 hospital, China.
authors.forename: Qinghong
authors.initials: Q
authors.lastname: Meng
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013395:Sucrose / Q000032:analysis
chemical_list: D004365:Drugs, Chinese Herbal; D012117:Resins, Synthetic; C534193:chuanxiong rhizome preparation; C027820:ligustilide; D013395:Sucrose; D015107:4-Butyrolactone
keywords: Chuanxiong Rhizoma; Discrimination; Fourier transform infrared spectroscopy (FT-IR); HPD-100 macroporous resin; Second derivative infrared spectroscopy (SD-IR); Two-dimensional correlation infrared spectroscopy (2DCOS-IR)