title: Expression of Translationally Controlled Tumor Protein in Human Kidney and in Renal Cell Carcinoma.
abstract: Translationally controlled tumor protein is a multifaceted protein involved in several physiological and biological functions. Its expression in normal kidney and in renal carcinomas, once corroborated by functional data, may add elements to elucidate renal physiology and carcinogenesis. In this study, translationally controlled tumor protein expression was evaluated by quantitative real time polymerase chain reaction and western blotting, and its localization was examined by immunohistochemistry on 84 nephrectomies for cancer. In normal kidney protein expression was found in the cytoplasm of proximal and distal tubular cells, in cells of the thick segment of the loop of Henle, and in urothelial cells of the pelvis. It was also detectable in cells of renal carcinoma with different pattern of localization (membranous and cytoplasmic) depending on tumor histotype. Our data may suggest an involvement of translationally controlled tumor protein in normal physiology and carcinogenesis. However, functional in vitro and in vivo studies are needed to verify this hypothesis.
authors.affiliation: Section of Pathology, Department of Medical Biotechnology, University of Siena, Via delle Scotte 6, 53100 Siena, Italy.
authors.forename: Maria R
authors.initials: MR
authors.lastname: Ambrosio
authors.affiliation: Section of Pathology, Department of Medical Biotechnology, University of Siena, Via delle Scotte 6, 53100 Siena, Italy ; Section of Pathology, Ospedale di Circolo di Busto Arsizio, Presidio Ospedaliero di Saronno, Piazzale Borella 1, 21047 Saronno, Italy.
authors.forename: Bruno J
authors.initials: BJ
authors.lastname: Rocca
authors.affiliation: Section of Pathology, Department of Medical Biotechnology, University of Siena, Via delle Scotte 6, 53100 Siena, Italy.
authors.forename: Aurora
authors.initials: A
authors.lastname: Barone
authors.affiliation: Section of Pathology, Department of Medical Biotechnology, University of Siena, Via delle Scotte 6, 53100 Siena, Italy ; Section of Pathology, Azienda Ospedaliera, "G. Salvini", Viale C. Forlanini 121, 20024 Garbagnate Milanese, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Onorati
authors.affiliation: Section of Pathology, Department of Medical Biotechnology, University of Siena, Via delle Scotte 6, 53100 Siena, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Mundo
authors.affiliation: Section of Pathology, Ospedale di Circolo di Busto Arsizio, Presidio Ospedaliero di Saronno, Piazzale Borella 1, 21047 Saronno, Italy.
authors.forename: Filippo
authors.initials: F
authors.lastname: Crivelli
authors.affiliation: Section of Pathology, Azienda Ospedaliera, "G. Salvini", Viale C. Forlanini 121, 20024 Garbagnate Milanese, Italy.
authors.forename: Franca
authors.initials: F
authors.lastname: Di Nuovo
authors.affiliation: Section of Pathology, Department of Medical Biotechnology, University of Siena, Via delle Scotte 6, 53100 Siena, Italy ; School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E14NS, UK.
authors.forename: Giulia
authors.initials: G
authors.lastname: De Falco
authors.affiliation: Department of Medicine, Science and Neurosciences, University of Siena, Via delle Scotte 6, 53100 Siena, Italy.
authors.forename: Maria T
authors.initials: MT
authors.lastname: del Vecchio
authors.affiliation: Section of Pathology, Azienda Ospedaliera Universitaria Senese, Viale Bracci 16, 53100 Siena, Italy.
authors.forename: Sergio A
authors.initials: SA
authors.lastname: Tripodi
authors.affiliation: Section of Pathology, Department of Medical Biotechnology, University of Siena, Via delle Scotte 6, 53100 Siena, Italy.
authors.forename: Piero
authors.initials: P
authors.lastname: Tosi
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D007680:Kidney Neoplasms / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D013194:Staining and Labeling; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D014408:Biomarkers, Tumor; D000090324:Tumor Protein, Translationally-Controlled 1