title: Design of a New Type of Compact Chemical Heater for Isothermal Nucleic Acid Amplification.
abstract: Previous chemical heater designs for isothermal nucleic acid amplification have been based on solid-liquid phase transition, but using this approach, developers have identified design challenges en route to developing a low-cost, disposable device. Here, we demonstrate the feasibility of a new heater configuration suitable for isothermal amplification in which one reactant of an exothermic reaction is a liquid-gas phase-change material, thereby eliminating the need for a separate phase-change compartment. This design offers potentially enhanced performance and energy density compared to other chemical and electric heaters.
authors.affiliation: Department of Bioengineering, Rice University, Houston, Texas, United States of America; PATH, Seattle, Washington, United States of America.
authors.forename: Kamal G
authors.initials: KG
authors.lastname: Shah
authors.affiliation: PATH, Seattle, Washington, United States of America.
authors.forename: Dylan
authors.initials: D
authors.lastname: Guelig
authors.affiliation: PATH, Seattle, Washington, United States of America.
authors.forename: Steven
authors.initials: S
authors.lastname: Diesburg
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, Washington, United States of America.
authors.forename: Joshua
authors.initials: J
authors.lastname: Buser
authors.affiliation: PATH, Seattle, Washington, United States of America.
authors.forename: Robert
authors.initials: R
authors.lastname: Burton
authors.affiliation: PATH, Seattle, Washington, United States of America.
authors.forename: Paul
authors.initials: P
authors.lastname: LaBarre
authors.affiliation: Department of Bioengineering, Rice University, Houston, Texas, United States of America.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Richards-Kortum
authors.affiliation: PATH, Seattle, Washington, United States of America.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Weigl
mesh_terms: D000531:Altitude; D002712:Chlorides; D003300:Copper; D003906:Developing Countries; D004867:Equipment Design; D006361:Heating / Q000295:instrumentation*; D006358:Hot Temperature; D008274:Magnesium; D000432:Methanol; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D010084:Oxidation-Reduction; D012965:Sodium Chloride; D014835:Volatilization; D014867:Water
chemical_list: D002712:Chlorides; D014867:Water; D012965:Sodium Chloride; D003300:Copper; D008274:Magnesium; C029892:cupric chloride; D000432:Methanol