title: A Conserved Core of Programmed Cell Death Indicator Genes Discriminates Developmentally and Environmentally Induced Programmed Cell Death in Plants.
abstract: A plethora of diverse programmed cell death (PCD) processes has been described in living organisms. In animals and plants, different forms of PCD play crucial roles in development, immunity, and responses to the environment. While the molecular control of some animal PCD forms such as apoptosis is known in great detail, we still know comparatively little about the regulation of the diverse types of plant PCD. In part, this deficiency in molecular understanding is caused by the lack of reliable reporters to detect PCD processes. Here, we addressed this issue by using a combination of bioinformatics approaches to identify commonly regulated genes during diverse plant PCD processes in Arabidopsis (Arabidopsis thaliana). Our results indicate that the transcriptional signatures of developmentally controlled cell death are largely distinct from the ones associated with environmentally induced cell death. Moreover, different cases of developmental PCD share a set of cell death-associated genes. Most of these genes are evolutionary conserved within the green plant lineage, arguing for an evolutionary conserved core machinery of developmental PCD. Based on this information, we established an array of specific promoter-reporter lines for developmental PCD in Arabidopsis. These PCD indicators represent a powerful resource that can be used in addition to established morphological and biochemical methods to detect and analyze PCD processes in vivo and in planta.
authors.affiliation: Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.).
authors.forename: Yadira
authors.identifier: 0000-0001-6161-7053
authors.initials: Y
authors.lastname: Olvera-Carrillo
authors.affiliation: Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.).
authors.forename: Michiel
authors.initials: M
authors.lastname: Van Bel
authors.affiliation: Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.).
authors.forename: Tom
authors.initials: T
authors.lastname: Van Hautegem
authors.affiliation: Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.).
authors.forename: Matyáš
authors.initials: M
authors.lastname: Fendrych
authors.affiliation: Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.).
authors.forename: Marlies
authors.identifier: 0000-0003-0792-8736
authors.initials: M
authors.lastname: Huysmans
authors.affiliation: Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.).
authors.forename: Maria
authors.initials: M
authors.lastname: Simaskova
authors.affiliation: Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.).
authors.forename: Matthias
authors.initials: M
authors.lastname: van Durme
authors.affiliation: Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.).
authors.forename: Pierre
authors.initials: P
authors.lastname: Buscaill
authors.affiliation: Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.).
authors.forename: Susana
authors.initials: S
authors.lastname: Rivas
authors.affiliation: Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.).
authors.forename: Nuria S.
authors.initials: NS
authors.lastname: Coll
authors.affiliation: Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.).
authors.forename: Frederik
authors.identifier: 0000-0001-6565-5145
authors.initials: F
authors.lastname: Coppens
authors.affiliation: Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.).
authors.forename: Steven
authors.initials: S
authors.lastname: Maere
authors.affiliation: Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.) moritz.nowack@vib.be.
authors.forename: Moritz K.
authors.identifier: 0000-0001-8918-7577
authors.initials: MK
authors.lastname: Nowack
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000145:classification / Q000235:genetics*; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling / Q000145:classification / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018613:Microscopy, Confocal; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000706:statistics & numerical data; D016877:Oxidants / Q000494:pharmacology; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride / Q000494:pharmacology; D059467:Transcriptome / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D029681:Arabidopsis Proteins; D016877:Oxidants; D012965:Sodium Chloride; D006861:Hydrogen Peroxide