title: Protective effect of Astragalus polysaccharide on endothelial progenitor cells injured by thrombin.
abstract: Several studies have demonstrated that Astragalus polysaccharide (APS) has a protective effect on endothelial cells damaged by various factors. To examine the role of APS in the endothelial inflammatory response, rat bone marrow endothelial progenitor cells (EPCs) were isolated by density gradient centrifugation and identified by immunohistochemistry, then we established a model of inflammatory injury induced by thrombin and measured the effects of APS on EPC viability and proliferation by MTT assays. We also assayed the effect APS had on the inflammatory response, by examining the nuclear factor kappa B (NF-κB) signaling pathway, as well as the expression of intercellular adhesion molecule-1 (ICAM-1), vascular endothelial growth factor (VEGF) and its receptors Flt-1 and KDR. Results demonstrated that EPCs were damaged by thrombin, and APS appeared to inhibit this damage. APS suppressed thrombin-induced ICAM-1 expression by blocking NF-κB signaling in rat bone marrow EPCs, and up-regulating expression of VEGF and its receptors. We believed that APS may be used to treat and prevent EPC injury-related diseases.
authors.affiliation: First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, China.
authors.forename: Xinxia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, China.
authors.forename: Kannan
authors.initials: K
authors.lastname: Yao
authors.affiliation: Shanxi Hospital of Integrated Traditional and Western Medicine, Taiyuan, Shanxi 030000, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Ren
authors.affiliation: First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.affiliation: First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, China. Electronic address: kejiaoke0579@163.com.
authors.forename: Dingguo
authors.initials: D
authors.lastname: Yao
mesh_terms: D000818:Animals; D027883:Astragalus Plant / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D066026:Endothelial Progenitor Cells / Q000187:drug effects* / Q000378:metabolism*; D015870:Gene Expression; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D013917:Thrombin / Q000633:toxicity*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D011134:Polysaccharides; D020011:Protective Agents; D042461:Vascular Endothelial Growth Factor A; D018799:Intercellular Adhesion Molecule-1; D013917:Thrombin
keywords: Astragalus polysaccharide; Endothelial progenitor cells; NF-κB signaling; Thrombin; VEGF signaling