title: Effects of soluble and particulate Cr(VI) on genome-wide DNA methylation in human B lymphoblastoid cells.
abstract: Several previous studies highlighted the potential epigenetic effects of Cr(VI), especially DNA methylation. However, few studies have compared the effects of Cr(VI) on DNA methylation profiles between soluble and particulate chromate in vitro. Accordingly, Illumina Infinium Human Methylation 450K BeadChip array was used to analyze DNA methylation profiles of human B lymphoblastoid cells exposed to potassium dichromate or lead chromate, and the cell viability was also studied. Array based DNA methylation analysis showed that the impacts of Cr(VI) on DNA methylation were limited, only about 40 differentially methylated CpG sites, with an overlap of 15CpG sites, were induced by both potassium dichromate and lead chromate. The results of mRNA expression showed that after Cr(VI) treatment, mRNA expression changes of four genes (TBL1Y, FZD5, IKZF2, and KIAA1949) were consistent with their DNA methylation alteration, but DNA methylation changes of other six genes did not correlate with mRNA expression. In conclusion, both of soluble and particulate Cr(VI) could induce a small amount of differentially methylated sites in human B lymphoblastoid cells, and the correlations between DNA methylation changes and mRNA expression varied between different genes.
authors.affiliation: Institute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, People's Republic of China. Electronic address: jianlinlou@163.com.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Lou
authors.affiliation: Institute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Junqiang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Institute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Ju
authors.affiliation: Institute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.affiliation: Institute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Zhaoqiang
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Institute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Lingfang
authors.initials: L
authors.lastname: Feng
authors.affiliation: Institute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Jin
authors.affiliation: Institute of Occupational Disease, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, People's Republic of China. Electronic address: xingtyou@mail.hz.zj.cn.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
mesh_terms: D001402:B-Lymphocytes / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival; D002857:Chromium / Q000737:chemistry*; D018899:CpG Islands; D004247:DNA / Q000737:chemistry; D019175:DNA Methylation / Q000187:drug effects*; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015894:Genome, Human; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D012844:Sincalide / Q000378:metabolism; D018675:Toxicity Tests
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D002857:Chromium; C074702:chromium hexavalent ion; D004247:DNA; D012844:Sincalide
keywords: Cytotoxicity; DNA methylation; Hexavalent chromium; Human B lymphoblastoid cells