title: Separation and purification of four flavonol diglucosides from the flower of Meconopsis integrifolia by high-speed counter-current chromatography.
abstract: Flavonoids are the main components of Meconopsis integrifolia (Maxim.) Franch, which is a traditional Tibetan medicine. However, traditional chromatography separation requires a large quantity of raw M. integrifolia and is very time consuming. Herein, we applied high-speed counter-current chromatography in the separation and purification of flavonoids from the ethanol extracts of M. integrifolia flower. Ethyl acetate/n-butanol/water (2:3:5, v/v/v) was selected as the optimum solvent system to purify the four components, namely quercetin-3-O-β-d-glucopyrannosy-(1→6)-β-d-glucopyranoside (compound 1, 60 mg), quercetin 3-O-[2'''-O-acetyl-β-d-glucopyranosyl-(1→6)-β-d-glucopyranoside (compound 2, 40 mg), quercetin 3-O-[3'''-O-acetyl-β-d-glucopyranosyl-(1→6)-β-d-glucopyranoside (compound 3, 11 mg), and quercetin 3-O-[6'''-O-acetyl-β-d-glucopyranosyl-(1→6)-β-d-glucopyranoside (compound 4, 16 mg). Among the four compounds, 3 and 4 were new acetylated flavonol diglucosides. After the high-speed counter-current chromatography separation, the purities of the four flavonol diglucosides were 98, 95, 90, and 92%, respectively. The structures of these compounds were identified by mass spectrometry and NMR spectroscopy.
authors.affiliation: Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Hubei University of Traditional Chinese Medicine, Wuhan, Hubei, China.|Institute of Ethnic Medicine, Southwest University for Nationalities, Chengdu, Sichuan, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Institute of Ethnic Medicine, Southwest University for Nationalities, Chengdu, Sichuan, China.
authors.forename: Yatao
authors.initials: Y
authors.lastname: Han
authors.affiliation: Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Hubei University of Traditional Chinese Medicine, Wuhan, Hubei, China.
authors.forename: Keli
authors.initials: K
authors.lastname: Chen
authors.affiliation: Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Hubei University of Traditional Chinese Medicine, Wuhan, Hubei, China.
authors.forename: Bisheng
authors.initials: B
authors.lastname: Huang
authors.affiliation: Institute of Ethnic Medicine, Southwest University for Nationalities, Chengdu, Sichuan, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002845:Chromatography; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification*; D035264:Flowers / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D008958:Models, Molecular; D015394:Molecular Structure; D028101:Papaveraceae / Q000737:chemistry*
chemical_list: D044948:Flavonols; D005960:Glucosides
keywords: Flavonol diglucosides; High-speed counter-current chromatography; Meconopsis integrifolia (Maxim.) Franch; Tibetan medicine