title: In vitro degradability, bioactivity and primary cell responses to bone cements containing mesoporous magnesium-calcium silicate and calcium sulfate for bone regeneration.
abstract: Mesoporous calcium sulfate-based bone cements (m-CSBC) were prepared by introducing mesoporous magnesium-calcium silicate (m-MCS) with specific surface area (410.9 m² g(-1)) and pore volume (0.8 cm³ g(-1)) into calcium sulfate hemihydrate (CSH). The setting time of the m-CSBC was longer with the increase of m-MCS content while compressive strength decreased. The degradation ratio of m-CSBC increased from 48.6 w% to 63.5 w% with an increase of m-MCS content after soaking in Tris-HCl solution for 84 days. Moreover, the m-CSBC containing m-MCS showed the ability to neutralize the acidic degradation products of calcium sulfate and prevent the pH from dropping. The apatite could be induced on m-CSBC surfaces after soaking in SBF for 7 days, indicating good bioactivity. The effects of the m-CSBC on vitamin D3 sustained release behaviours were investigated. It was found that the cumulative release ratio of vitamin D3 from the m-CSBC significantly increased with the increase of m-MCS content after soaking in PBS (pH = 7.4) for 25 days. The m-CSBC markedly improved the cell-positive responses, including the attachment, proliferation and differentiation of MC3T3-E1 cells, suggesting good cytocompatibility. Briefly, m-CSBC with good bioactivity, degradability and cytocompatibility might be an excellent biocement for bone regeneration.
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
authors.forename: Yueting
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
authors.forename: Songchao
authors.initials: S
authors.lastname: Tang
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, People's Republic of China.
authors.forename: Baoqing
authors.initials: B
authors.lastname: Yu
authors.affiliation: College of Physical Science and Technology, Sichuan University, Chengdu 610041, People's Republic of China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Yan
authors.affiliation: College of Physical Science and Technology, Sichuan University, Chengdu 610041, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, People's Republic of China biomaterbone@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, People's Republic of China jiacansu@sina.com.
authors.forename: Jiacan
authors.initials: J
authors.lastname: Su
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001031:Apatites / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001843:Bone Cements / Q000737:chemistry*; D001861:Bone Regeneration; D017610:Calcium Compounds / Q000737:chemistry*; D002133:Calcium Sulfate / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002762:Cholecalciferol / Q000737:chemistry; D019245:Compressive Strength; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010011:Osteocytes / Q000166:cytology; D011208:Powders; D017640:Silicates / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D001843:Bone Cements; D017610:Calcium Compounds; D011208:Powders; D017640:Silicates; D002762:Cholecalciferol; D000469:Alkaline Phosphatase; D008274:Magnesium; C031293:calcium silicate; D002133:Calcium Sulfate
keywords: bone cement; calcium sulfate; cytocompatibility; degradability; mesoporous magnesium–calcium silicate