title: IGK with conserved IGΚV/IGΚJ repertoire is expressed in acute myeloid leukemia and promotes leukemic cell migration.
abstract: We have previously reported that immunoglobulin heavy chain genes were expressed in myeloblasts and mature myeloid cells. In this study, we further demonstrated that rearranged Ig κ light chain was also frequently expressed in acute myeloid leukemia cell lines (6/6), primary myeloblasts from patients with acute myeloid leukemia (17/18), and mature monocytes (11/12) and neutrophils (3/12) from patients with non-hematopoietic neoplasms, but not or only rarely expressed in mature neutrophils (0/8) or monocytes (1/8) from healthy individuals. Interestingly, myeloblasts and mature monocytes/neutrophils shared several restricted IGKV and IGKJ gene usages but with different expression frequency. Surprisingly, almost all of the acute myeloid leukemia-derived IGKV showed somatic hypermutation; in contrast, mature myeloid cells-derived IGKV rarely had somatic hypermutation. More importantly, although IGK expression appeared not to affect cell proliferation, reduced IGK expression led to a decrease in cell migration in acute myeloid leukemia cell lines HL-60 and NB4, whereas increased IGK expression promoted their motility. In summary, IGK is expressed in myeloblasts and mature myeloid cells from patients with non-hematopoietic neoplasms, and is involved in cell migration. These results suggest that myeloid cells-derived IgK may have a role in leukemogenesis and may serve as a novel tumor marker for monitoring minimal residual disease and developing target therapy.
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing, China.|Department of Hematopathology, UT MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Miaoran
authors.initials: M
authors.lastname: Xia
authors.affiliation: Department of Laboratory Medicine, UT MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Hematopathology, UT MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Zhiqiao
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing, China.
authors.forename: Fanlei
authors.initials: F
authors.lastname: Hu
authors.affiliation: Department of Pathology, University of Texas-Houston Medical School, Houston, TX, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Hematopathology, UT MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Bueso-Ramos
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing, China.|Department of Hematopathology, UT MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Department of Hematopathology, UT MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: C Cameron
authors.initials: CC
authors.lastname: Yin
mesh_terms: D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000276:immunology*; D049109:Cell Proliferation / Q000502:physiology; D015321:Gene Rearrangement; D018922:HL-60 Cells; D006801:Humans; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000235:genetics; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D008969:Molecular Sequence Data; D014162:Transfection
chemical_list: C014609:IgK; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins
keywords: IgK; acute myeloid leukemia; leukemic cell migration; restricted IGKV/IGKJ rearrangement; somatic hypermutation