title: Association of NCOA3 polymorphisms with Dyslipidemia in the Chinese Han population.
abstract: BACKGROUND
  Nuclear receptor coactivator-3 (NCOA3) is involved in various physiological processes. Emerging evidence from previous studies using animal models suggests that the NCOA3 gene (NCOA3) plays a critical role in lipid metabolism as well as adipogenesis and obesity. The present study aims to investigate the association between NCOA3 SNPs and dyslipidemia in the Chinese Han population.
  
  
  METHODS
  Five hundred and twenty-nine (529) Chinese Han subjects were recruited. Four tag SNPs (rs2425955G > T, rs6066394T > C, rs10485463C > G, and rs6094753G > A) in NCOA3, selected from the HapMap website, were genotyped using MALDI-TOF mass spectrometry. Data analysis was performed using SPSS 16.0, SNPStats and haploview 4.2.
  
  
  RESULTS
  Four SNPs (rs2425955, rs6066394, rs10485463, and rs6094753) were associated with triglyceride levels. Except for SNP rs10485463, genotype distributions and allele frequencies of the other three NCOA3 SNPs (rs2425955, rs6066394, and rs6094753) were significantly different between hypertriglyceridemia subjects and normal group. Significant differences were also observed in allele frequencies and genotype distributions of SNP rs10485463 between low-HDL cholesterolemia subjects and normal group. Carriers of rs2425955 T allele had a lower risk of hypertriglyceridemia compared to GG genotype. Similar results were observed from rs6094753. Subjects with rs6066394 CT genotype had a lower risk of hypertriglyceridemia than those with the TT genotype; however, CC and TT genotypes showed no significant difference in the risk of hypertriglyceridemia. Similar results were found in the association between rs6066394 and hypercholesterolemia. The variant alleles of rs2425955, rs6066394 and rs6094753 were associated with a lower risk of hypertriglyceridemia compared with the wild-type alleles. The G allele of rs10485463 was associated with an increased risk of low-HDL cholesterolemia. In the log-additive model the association between rs2425955 and hypertriglyceridemia remained significant after Bonferroni correction, and genotypes with variant alleles were associated with a lower risk of hypertriglyceridemia.
  
  
  CONCLUSIONS
  In summary, this study demonstrated that variation in NCOA3 might influence the risk of dyslipidemia and serum lipid levels in Chinese Han population.
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, 130021, China. 1076158138@qq.com.
authors.forename: Mingxi
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, 130021, China. 653875336@qq.com.
authors.forename: Siame
authors.initials: S
authors.lastname: Gilbert
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, 130021, China. l_yong@jlu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Psychiatry, Yale University School of Medicine, VA Medical, Center/116A2, 950 Campbell Avenue, West Haven, CT, 06516, USA. huiping.zhang@yale.edu.
authors.forename: Huiping
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, 130021, China. stacy_1985@163.com.
authors.forename: Yichun
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, 130021, China. 810456330@qq.com.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, 130021, China. tangyuan86301@126.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, 130021, China. 853970918@qq.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhen
authors.affiliation: The Cardiovascular Center, The First Hospital of Jilin University, Changchun, 130021, China. chengyi@jlu.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, 130021, China. ywliu@jlu.edu.cn.
authors.forename: Yawen
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D050171:Dyslipidemias / Q000097:blood / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D050356:Lipid Metabolism / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D056921:Nuclear Receptor Coactivator 3 / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014280:Triglycerides / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D014280:Triglycerides; C538866:NCOA3 protein, human; D056921:Nuclear Receptor Coactivator 3