title: Embryonic Stem Cell-Derived Microvesicles: Could They be Used for Retinal Regeneration?
abstract: Mouse embryonic stem cells (mESCs) release into the medium in which they are cultured heterogeneous populations of microvesicles (mESMVs), important components of cell-cell communication, that transfer their contents not only to other stem cells but also to cells of other origins. The purpose of these studies was to demonstrate that ESMVs could be the signals that lead the retinal progenitor Müller cells to de-differentiate and re-entry the cell cycle, followed by differentiation along retinal lineages. Indeed, we found that ESMVs induce these processes and change Müller cells' microenvironment towards a more permissive state for tissue regeneration.
authors.affiliation: Stein Eye Institute, David Geffen School of Medicine, University of California Los Angeles, 90095-7000, Los Angeles, CA, USA. farber@jsei.ucla.edu.|Department of Ophthalmology, David Geffen School of Medicine, University of California Los Angeles, 90095-7000, Los Angeles, CA, USA. farber@jsei.ucla.edu.|Molecular Biology Institute, Paul Boyer Hall, University of California Los Angeles, 90095, Los Angeles, CA, USA. farber@jsei.ucla.edu.|Brain Research Institute, University of California Los Angeles, 90095, Los Angeles, CA, USA. farber@jsei.ucla.edu.
authors.forename: Debora B
authors.initials: DB
authors.lastname: Farber
authors.affiliation: Stein Eye Institute, David Geffen School of Medicine, University of California Los Angeles, 90095-7000, Los Angeles, CA, USA. katsman@jsei.ucla.edu.|Department of Ophthalmology, David Geffen School of Medicine, University of California Los Angeles, 90095-7000, Los Angeles, CA, USA. katsman@jsei.ucla.edu.
authors.forename: Diana
authors.initials: D
authors.lastname: Katsman
mesh_terms: D000818:Animals; D054337:Cell Dedifferentiation / Q000235:genetics / Q000502:physiology; D055252:Cell-Derived Microparticles / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D063928:Ependymoglial Cells / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012038:Regeneration / Q000235:genetics / Q000502:physiology*; D012160:Retina / Q000378:metabolism / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D050814:Octamer Transcription Factor-3; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins
keywords: De-differentiation; Differentiation; Embryonic stem cells; Müller cell cultures; Müller progenitor cells; Retina; Retina regeneration; Retinal cell lineages; Stem cell microvesicles; Stem cells