title: The application of terminomics for the identification of protein start sites and proteoforms in bacteria.
abstract: Protein terminomics, or the study of amino acids sequences at the protein amino or carboxyl terminus has rapidly evolved as a proteomic discipline due to significant methodological improvements in the labelling and recovery of terminal peptides as well as the increased speed and sensitivity of current mass spectrometry instrumentation. The most significant beneficiaries of these developments include an increased awareness and understanding of complex proteolytic cascades that regulate key biological processes and in genome annotation. Most terminomics research to date has focused on gaining insight into important biological processes such as inflammation, wound healing and cancer. The application of terminomics to the study of important biological questions in prokaryotes is gaining traction. Here we review current applications and progress of terminomics in prokaryotes, discuss the significance of protease research in bacterial pathogenesis and protein maturation, and suggest novel applications of terminomics in the study of infectious disease.
authors.affiliation: The ithree Institute, University of Technology Sydney, Broadway, NSW, Australia.|Proteomics Core Facility, University of Technology Sydney, Broadway, NSW, Australia.
authors.forename: Iain J
authors.initials: IJ
authors.lastname: Berry
authors.affiliation: Proteomics Core Facility, University of Technology Sydney, Broadway, NSW, Australia.
authors.forename: Joel R
authors.initials: JR
authors.lastname: Steele
authors.affiliation: The ithree Institute, University of Technology Sydney, Broadway, NSW, Australia.|Proteomics Core Facility, University of Technology Sydney, Broadway, NSW, Australia.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Padula
authors.affiliation: The ithree Institute, University of Technology Sydney, Broadway, NSW, Australia.|Proteomics Core Facility, University of Technology Sydney, Broadway, NSW, Australia.
authors.forename: Steven P
authors.initials: SP
authors.lastname: Djordjevic
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016680:Genome, Bacterial; D006801:Humans; D013058:Mass Spectrometry; D016366:Open Reading Frames; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals; D040901:Proteomics / Q000379:methods; D020539:Sequence Analysis, Protein; D024363:Transcription Initiation Site
chemical_list: D001426:Bacterial Proteins; D020033:Protein Isoforms; D021382:Protein Sorting Signals
keywords: Degradomics; Limited proteolysis; Microbiology; Post-translational cleavage; Proteogenomics; Terminomics