title: Rapid low dose electron tomography using a direct electron detection camera.
abstract: We demonstrate the ability to record a tomographic tilt series containing 3487 images in only 3.5 s by using a direct electron detector in a transmission electron microscope. The electron dose is lower by at least one order of magnitude when compared with that used to record a conventional tilt series of fewer than 100 images in 15-60 minutes and the overall signal-to-noise ratio is greater than 4. Our results, which are illustrated for an inorganic nanotube, are important for ultra-low-dose electron tomography of electron-beam-sensitive specimens and real-time dynamic electron tomography of nanoscale objects with sub-ms temporal resolution.
authors.affiliation: Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons and Peter Grünberg Institute, Forschungszentrum Jülich, D-52425 Jülich, Germany.
authors.forename: Vadim
authors.initials: V
authors.lastname: Migunov
authors.affiliation: PNSensor GmbH, Otto-Hahn-Ring 6, 81739 München, Germany.
authors.forename: Henning
authors.initials: H
authors.lastname: Ryll
authors.affiliation: Centrum Wiskunde &Informatica, P.O. Box 94079, NL-1090 GB Amsterdam, The Netherlands.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhuge
authors.affiliation: PNDetector GmbH, Otto-Hahn-Ring 6, 81739 München, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Simson
authors.affiliation: PNSensor GmbH, Otto-Hahn-Ring 6, 81739 München, Germany.|University of Siegen, Walter Flex Str. 3, 57068 Siegen, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Strüder
authors.affiliation: Centrum Wiskunde &Informatica, P.O. Box 94079, NL-1090 GB Amsterdam, The Netherlands.|Mathematical Institute, Leiden University, The Netherlands.|iMinds-Vision Lab, University of Antwerp, Belgium.
authors.forename: K Joost
authors.initials: KJ
authors.lastname: Batenburg
authors.affiliation: Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons and Peter Grünberg Institute, Forschungszentrum Jülich, D-52425 Jülich, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Houben
authors.affiliation: Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons and Peter Grünberg Institute, Forschungszentrum Jülich, D-52425 Jülich, Germany.
authors.forename: Rafal E
authors.initials: RE
authors.lastname: Dunin-Borkowski