title: Observing and Quantifying Fluorescent Reporters.
abstract: Genetically encoded fluorescent reporters take advantage of C. elegans' transparency to allow noninvasive, in vivo observation and recording of physiological processes in intact animals. Here, I discuss the basic microscope components required to observe, image, and measure fluorescent proteins in live animals for students and researchers who work with C. elegans but have limited experience with fluorescence imaging and analysis.
authors.affiliation: Department of Biology, McGill University, 1205 Ave Docteur Penfield, Rm N5/12, Montreal, QC, Canada, H3A 1B1. michael.hendricks@mcgill.ca.
authors.forename: Michael
authors.initials: M
authors.lastname: Hendricks
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics; D015870:Gene Expression; D017930:Genes, Reporter; D008164:Luminescent Proteins / Q000235:genetics*; D008856:Microscopy, Fluorescence / Q000379:methods
chemical_list: D008164:Luminescent Proteins
keywords: Digital image analysis; Fluorescent proteins; Microscopy