title: The anti-aging properties of a human placental hydrolysate combined with dieckol isolated from Ecklonia cava.
abstract: In the present study, we aimed to examine the anti-aging properties of human placental hydrolysate (HPE) and dieckol (DE) from Ecklonia cava against free radical scavenging, muscle hypertrophy-related follistatin mRNA expression, amelioration of cognition-related genes and proteins, inhibition of collagenase-regulating genes, and elastinase activity.
  
  
  
  The anti-aging effects were examined in human fibroblast (CCD986sk), mouse myoblast (C2C12), and neuroblastoma (N2a) cell models, by employing various assays such as 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) scavenging, hydroxyl radical-mediated oxidation, quantitative real-time polymerase chain reaction, enzyme activity, and immunocytochemistry observation.
  
  
  
  Our results show that HPE combined with DE (HPE:DE) strongly scavenged DPPH radicals and protected proteins against degradation by hydroxyl radical attack. HPE:DE effectively inhibited matrix metalloproteinase-1 expression, protein kinase C alpha expression, and elastinase activity. Furthermore, HPE:DE improved the expression of cognition-related genes (choline acetyltransferase and vesicular acetylcholine transporter). These events may proactively contribute to retard the aging processes and the abrupt physiological changes probably induced by mitochondrial dysfunction with aging.
  
  
  
  Based on these findings, we conclude that the combined treatment of HPE:DE may be useful for anti-aging therapy in which the accumulation of oxidative damage is the main driving force.
authors.affiliation: Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University, 120 Gangneung Daehangno, Gangneung, Gangwon, 210-702, Republic of Korea.
authors.forename: Su Kil
authors.initials: SK
authors.lastname: Jang
authors.affiliation: College of Pharmacy, Chung-Ang University, Heuksuk-dong, Dongjak-gu, Seoul, 156-756, Republic of Korea.
authors.forename: Do Ik
authors.initials: DI
authors.lastname: Lee
authors.affiliation: Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University, 120 Gangneung Daehangno, Gangneung, Gangwon, 210-702, Republic of Korea.
authors.forename: Seung Tae
authors.initials: ST
authors.lastname: Kim
authors.affiliation: Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University, 120 Gangneung Daehangno, Gangneung, Gangwon, 210-702, Republic of Korea.
authors.forename: Gwang Hoon
authors.initials: GH
authors.lastname: Kim
authors.affiliation: Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University, 120 Gangneung Daehangno, Gangneung, Gangwon, 210-702, Republic of Korea.
authors.forename: Da Woon
authors.initials: da W
authors.lastname: Park
authors.affiliation: Industry-Academic Cooperation Foundation, Hanbat National University, Daejeon, 305-719, Republic of Korea.
authors.forename: Jung Youl
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University, 120 Gangneung Daehangno, Gangneung, Gangwon, 210-702, Republic of Korea.
authors.forename: Daehee
authors.initials: D
authors.lastname: Han
authors.affiliation: Central Research Institute, Dr. Chung's Food Co. Ltd., Chungbuk, 361-782, Republic of Korea.
authors.forename: Jae Kwon
authors.initials: JK
authors.lastname: Choi
authors.affiliation: Central Research Institute, Dr. Chung's Food Co. Ltd., Chungbuk, 361-782, Republic of Korea.
authors.forename: Yoon-bok
authors.initials: YB
authors.lastname: Lee
authors.affiliation: Chungbuk National University, Chungbuk, 361-763, Republic of Korea.
authors.forename: Nam-Soo
authors.initials: NS
authors.lastname: Han
authors.affiliation: Chungbuk National University, Chungbuk, 361-763, Republic of Korea.
authors.forename: Yun Bae
authors.initials: YB
authors.lastname: Kim
authors.affiliation: DF-Dr. Han Biotech., Shaoyaojubeili, Chaoyang District, Beijing, 10029, China.
authors.forename: Jeongsu
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University, 120 Gangneung Daehangno, Gangneung, Gangwon, 210-702, Republic of Korea. ssj66@gwnu.ac.kr.
authors.forename: Seong Soo
authors.initials: SS
authors.lastname: Joo
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001572:Benzofurans / Q000494:pharmacology*; D002460:Cell Line; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D000459:Phaeophyceae / Q000737:chemistry*; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D011492:Protein Hydrolysates / Q000494:pharmacology*; D051571:Protein Kinase C-alpha / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D001572:Benzofurans; D016166:Free Radical Scavengers; D011492:Protein Hydrolysates; D017382:Reactive Oxygen Species; C503840:dieckol; D051571:Protein Kinase C-alpha; D020781:Matrix Metalloproteinase 1