title: Expression and Localization of Aquaporin 4 and Aquaporin 5 along the Large Intestine of Colostrum-Suckling Buffalo Calves.
abstract: Aquaporins (AQPs) are membrane channel proteins that play a role in regulating water permeability in many tissues. To date, seven isoforms of AQPs have been reported in the gastrointestinal tract in different mammalian species. In contrast, both tissue distribution and expression of AQPs are unknown in the buffalo. The purpose of this study was to investigate the expression of both AQP4 and AQP5 mRNAs and their relative proteins in the large intestinal tracts of buffalo calves after colostrum suckling using reverse transcriptase polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry. Our results revealed a diversified tissue AQP4 and AQP5 immunolocalization accompanied by their highest expression in the tissues of colostrum-suckling buffalo calves confirmed by Western blotting. In particular, AQP4 was distributed along the endothelium and enterocytes while AQP5 in the endocrine cells. These findings provide direct evidence for AQP4 and AQP5 expression in the large intestine, suggesting that different AQPs collaborate functionally and distinctively in water handling during intestinal development, especially during the first period after delivery.
authors.affiliation: Department of Advanced Biomedical Sciences, University of Naples Federico II, Via Pansini 5, Naples, 80131, Italy. alpelaga@unina.it.|Institute of Biostructures and Bioimages, National Research Council, Via De Amicis 95, Naples, 80131, Italy. alpelaga@unina.it.
authors.forename: A
authors.initials: A
authors.lastname: Pelagalli
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria, Naples, 80137, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Squillacioti
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria, Naples, 80137, Italy.
authors.forename: A
authors.initials: A
authors.lastname: De Luca
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria, Naples, 80137, Italy.
authors.forename: M E
authors.initials: ME
authors.lastname: Pero
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria, Naples, 80137, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Vassalotti
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria, Naples, 80137, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Lombardi
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria, Naples, 80137, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Avallone
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria, Naples, 80137, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Mirabella
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D051401:Aquaporin 4 / Q000235:genetics / Q000378:metabolism*; D051402:Aquaporin 5 / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport / Q000502:physiology; D015153:Blotting, Western / Q000662:veterinary; D002020:Buffaloes / Q000378:metabolism*; D003126:Colostrum; D055098:Endocrine Cells / Q000378:metabolism*; D004727:Endothelium / Q000378:metabolism*; D020895:Enterocytes / Q000378:metabolism*; D007150:Immunohistochemistry / Q000662:veterinary; D007420:Intestine, Large / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary*; D014867:Water / Q000378:metabolism
chemical_list: D051401:Aquaporin 4; D051402:Aquaporin 5; D012333:RNA, Messenger; D014867:Water