title: Mesenchymal Stem Cells Derived from Human Adipose Tissue.
abstract: Human adult mesenchymal stem cells are present in fat tissue, which can be obtained using surgical procedures such as liposuction. The multilineage capacity of mesenchymal stem cells makes them very valuable for cell-based medical therapies. In this chapter, we describe how to isolate mesenchymal stem cells from human adult fat tissue, propagate the cells in culture, and cryopreserve the cells for tissue engineering applications. Flow cytometry methods are also described for identification and characterization of adipose-derived stem cells and for cell sorting.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
authors.forename: Nastaran
authors.initials: N
authors.lastname: Mahmoudifar
authors.affiliation: Faculty of Science, Engineering and Technology, Swinburne University of Technology, P.O. Box 218, Hawthorn, Melbourne, VIC, 3122, Australia. pdoran@swin.edu.au.
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Doran
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D053687:Adult Stem Cells / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D020219:Chondrogenesis; D015925:Cryopreservation; D005434:Flow Cytometry; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D010641:Phenotype; D044968:Regenerative Medicine / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D015415:Biomarkers
keywords: Cell expansion; Cell isolation; Flow cytometry; Human adipose tissue; Mesenchymal stem cells; Stem cell culture