title: Detection of Intraplaque Hemorrhage in Mouse Atherosclerotic Lesions.
abstract: Intraplaque hemorrhage is defined as the presence of fresh or lysed erythrocytes, iron deposits in macrophages, and/or a fibrin clot in an atherosclerotic plaque. These features can be detected by hematoxylin and eosin, Martius scarlet Blue, and Perl's iron histological stainings. It is noteworthy that intraplaque hemorrhage is only present in murine atherosclerotic plaques after additional interventions or additional genetic traits affecting matrix degradation or thrombosis. In this chapter, we describe methods to detect intraplaque hemorrhage in mouse atherosclerotic lesions.
authors.affiliation: Department of Pathology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, 5800, 6202 AZ, The Netherlands.
authors.forename: Judith C
authors.initials: JC
authors.lastname: Sluimer
authors.affiliation: Department of Pathology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, 5800, 6202 AZ, The Netherlands.|Department of Molecular Genetics, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands.|Department of Medical Biochemistry, Amsterdam Medical Center, Amsterdam, The Netherlands.
authors.forename: Marion J
authors.initials: MJ
authors.lastname: Gijbels
authors.affiliation: Department of Pathology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, 5800, 6202 AZ, The Netherlands. s.heeneman@maastrichtuniversity.nl.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Heeneman
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000473:pathology*; D001018:Aortic Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D001057:Apolipoproteins E / Q000172:deficiency / Q000235:genetics; D050197:Atherosclerosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D001391:Azo Compounds; D004396:Coloring Agents; D004195:Disease Models, Animal; D004801:Eosine Yellowish-(YS); D005295:Ferrocyanides; D020022:Genetic Predisposition to Disease; D006416:Hematoxylin; D006470:Hemorrhage / Q000473:pathology*; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype; D058226:Plaque, Atherosclerotic; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D001057:Apolipoproteins E; D001391:Azo Compounds; D004396:Coloring Agents; D005295:Ferrocyanides; C042643:Martius scarlet blue trichrome; D004801:Eosine Yellowish-(YS); C000170:ferric ferrocyanide; D006416:Hematoxylin
keywords: Erythrocytes; Fibrin; Hematoxylin and eosin; Intraplaque hemorrhage; Iron; Martius scarlet Blue; Perl’s Prussian blue