title: Effect of sulfide, osmotic, and thermal stresses on taurine transporter mRNA levels in the gills of the hydrothermal vent-specific mussel Bathymodiolus septemdierum.
abstract: Hydrothermal vent environmental conditions are characterized by high sulfide concentrations, fluctuating osmolality, and irregular temperature elevations caused by vent effluents. These parameters represent potential stressors for organisms that inhabit the area around hydrothermal vents. Here, we aimed to obtain a better understanding of the adaptation mechanisms of marine species to hydrothermal vent environments. Specifically, we examined the effect of sulfide, osmolality, and thermal stress on the expression of taurine transporter (TAUT) mRNA in the gill of the deep-sea mussel Bathymodiolus septemdierum, which is a dominant species around hydrothermal vent sites. We analyzed TAUT mRNA levels by quantitative real-time polymerase chain reaction (PCR) in the gill of mussels exposed to sulfide (0.1 or 1mg/L Na2S·9H2O), hyper- (115% seawater) and hypo- (97.5%, 95.5%, and 85% seawater) osmotic conditions, and thermal stresses (12°C and 20°C) for 24 and 48h. The results showed that mussels exposed to relatively low levels of sulfide (0.1mg/L) and moderate heat stress (12°C) exhibited higher TAUT mRNA levels than the control. Although hyper- and hypo-osmotic stress did not significantly change TAUT mRNA levels, slight induction was observed in mussels exposed to low osmolality. Our results indicate that TAUT is involved in the coping mechanism of mussels to various hydrothermal vent stresses.
authors.affiliation: Department of Marine Bioscience, Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwa, Chiba 277-8564, Japan. Electronic address: ikumik@aori.u-tokyo.ac.jp.
authors.forename: Ikumi
authors.initials: I
authors.lastname: Nakamura-Kusakabe
authors.affiliation: Department of Marine Bioscience, Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwa, Chiba 277-8564, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nagasaki
authors.affiliation: Department of Marine Bioscience, Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwa, Chiba 277-8564, Japan.
authors.forename: Azusa
authors.initials: A
authors.lastname: Kinjo
authors.affiliation: Department of Marine Bioscience, Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwa, Chiba 277-8564, Japan.
authors.forename: Mieko
authors.initials: M
authors.lastname: Sassa
authors.affiliation: College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Koito
authors.affiliation: Center for Advanced Marine Core Research, Kochi University, Nankoku, Kochi 783-8502, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Okamura
authors.affiliation: Graduate School of Natural Science and Technology, Okayama University, Okayama, Okayama 700-8530, Japan.
authors.forename: Shosei
authors.initials: S
authors.lastname: Yamagami
authors.affiliation: Graduate School of Natural Science and Technology, Okayama University, Okayama, Okayama 700-8530, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Yamanaka
authors.affiliation: Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka 237-0061, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Tsuchida
authors.affiliation: Department of Marine Bioscience, Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwa, Chiba 277-8564, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Inoue
mesh_terms: D000818:Animals; D005880:Gills / Q000187:drug effects / Q000378:metabolism*; D018869:Heat-Shock Response / Q000187:drug effects* / Q000235:genetics; D060086:Hydrothermal Vents; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D049879:Mytilidae / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D009994:Osmolar Concentration; D009997:Osmotic Pressure / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013440:Sulfides / Q000494:pharmacology*; D013696:Temperature; D000099247:Taurine Transporters
chemical_list: D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D013440:Sulfides; D000099247:Taurine Transporters; C033479:sodium sulfide
keywords: Deep sea; Environmental adaptation; Hypotaurine; Marine invertebrate; Osmoregulation; Taurine transporter