title: Solid-Phase Peptide-Carrier Conjugation.
abstract: Conjugation to carrier proteins is necessary for peptides to be able to induce antibody formation when injected into animals together with a suitable adjuvant. This is usually performed by conjugation in solution followed by mixing with the adjuvant. Alternatively, the carrier may be adsorbed onto a solid support followed by activation and conjugation with the peptide by solid-phase chemistry. Different reagents can be used for conjugation through peptide functional groups (-SH, -NH2, -COOH) and various carrier proteins may be used depending on the peptides and the intended use of the antibodies. The solid phase may be an ion-exchange matrix, from which the conjugate can subsequently be eluted and mixed with adjuvant. Alternatively, the adjuvant aluminum hydroxide may be used as the solid-phase matrix, whereupon the carrier is immobilized and conjugated with peptide. The resulting adjuvant-carrier-peptide complexes may then be used directly for immunization.
authors.affiliation: Department of Autoimmunology and Biomarkers, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark. gh@ssi.dk.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Houen
authors.affiliation: Department of Autoimmunology and Biomarkers, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark.
authors.forename: Dorthe T
authors.initials: DT
authors.lastname: Olsen
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry; D000536:Aluminum Hydroxide / Q000737:chemistry; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation; D002352:Carrier Proteins / Q000737:chemistry* / Q000276:immunology; D007114:Immunization; D051379:Mice; D010455:Peptides / Q000737:chemistry* / Q000276:immunology; D011817:Rabbits; D060327:Solid-Phase Synthesis Techniques
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; D002352:Carrier Proteins; D010455:Peptides; D000536:Aluminum Hydroxide
keywords: Antibodies; Carrier; Conjugation; Peptide; Solid phase