title: Time-course changes of hippocalcin expression in the mouse hippocampus following pilocarpine-induced status epilepticus.
abstract: Hippocalcin participates in the maintenance of neuronal calcium homeostasis. In the present study, we examined the time-course changes of neuronal degeneration and hippocalcin protein level in the mouse hippocampus following pilocarpine-induced status epilepticus (SE). Marked neuronal degeneration was observed in the hippocampus after SE in a time-dependent manner, although neuronal degeneration differed according to the hippocampal subregions. Almost no hippocalcin immunoreactivity was detected in the pyramidal neurons of the cornu ammonis 1 (CA1) region from 6 h after SE. However, many pyramidal neurons in the CA2 region showed hippocalcin immunoreactivity until 24 h after SE. In the CA3 region, only a few hippocalcin immunoreactive cells were observed at 12 h after SE, and almost no hippocalcin immunoreactivity was observed in the pyramidal neurons from 24 h after SE. Hippocalcin immunoreactivity in the polymorphic cells of the dentate gyrus was markedly decreased from 6 h after SE. In addition, hippocalcin protein level in the hippocampus began to decrease from 6 h after SE, and was significantly decreased at 24 h and 48 h after pilocarpine-induced SE. These results indicate that marked reduction of hippocalcin level may be closely related to neuronal degeneration in the hippocampus following pilocarpine-induced SE.
authors.affiliation: Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan 31116, Korea.
authors.forename: Hee-Soo
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan 31116, Korea.
authors.forename: Choong-Hyun
authors.initials: CH
authors.lastname: Lee
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D051597:Hippocalcin / Q000235:genetics* / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009410:Nerve Degeneration / Q000139:chemically induced / Q000503:physiopathology*; D010862:Pilocarpine / Q000494:pharmacology; D013226:Status Epilepticus / Q000139:chemically induced / Q000503:physiopathology*; D013997:Time Factors
chemical_list: D010862:Pilocarpine; D051597:Hippocalcin
keywords: hippocalcin; hippocampus; neuronal degeneration; status epilepticus