title: The potential role of the NEK6, AURKA, AURKB, and PAK1 genes in adenomatous colorectal polyps and colorectal adenocarcinoma.
abstract: Colorectal adenomatous polyp (CRAP) is a major risk factor for the development of sporadic colorectal cancer (CRC). Histone modifications are one of the epigenetic mechanisms that may have key roles in the carcinogenesis of CRC. The objective of the present study is to investigate the alternations in the defined histone modification gene expression profiles in patients with CRAP and CRC. Histone modification enzyme key gene expressions of the CRC, CRAP, and control groups were evaluated and compared using the reverse transcription PCR (RT-PCR) array method. Gene expression analysis was performed in the CRAP group after dividing the patients into subgroups according to the polyp diameter, pathological results, and morphological parameters which are risk factors for developing CRC in patients with CRAP. PAK1, NEK6, AURKA, AURKB, HDAC1, and HDAC7 were significantly more overexpressed in CRC subjects compared to the controls (p < 0.05). PAK1, NEK6, AURKA, AURKB, and HDAC1 were significantly more overexpressed in the CRAP group compared to the controls (p < 0.005). There were no significant differences between the CRAP and CRC groups with regards to PAK1, NEK6, AURKA, or AURKB gene overexpression. PAK1, NEK6, AURKA, and AURKB were significantly in correlation with the polyp diameter as they were more overexpressed in polyps with larger diameters. In conclusion, overexpressions of NEK6, AURKA, AURKB, and PAK1 genes can be used as predictive markers to decide the colonoscopic surveillance intervals after the polypectomy procedure especially in polyps with larger diameters.
authors.affiliation: Department of Gastroenterology, Medical Faculty, Celal Bayar University, Manisa, Turkey. elmaskasap@yahoo.com.
authors.forename: Elmas
authors.initials: E
authors.lastname: Kasap
authors.affiliation: Department of Gastroenterology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Gerceker
authors.affiliation: Department of Medical Genetics and Medical Biology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Seda Örenay
authors.initials: SÖ
authors.lastname: Boyacıoglu
authors.affiliation: Department of Gastroenterology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Yuceyar
authors.affiliation: Department of Medical Genetics and Medical Biology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Hatice
authors.initials: H
authors.lastname: Yıldırm
authors.affiliation: Department of Pathology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Semin
authors.initials: S
authors.lastname: Ayhan
authors.affiliation: Department of Medical Genetics and Medical Biology, Medical Faculty, Celal Bayar University, Manisa, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Korkmaz
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D011125:Adenomatous Polyposis Coli / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D064096:Aurora Kinase A / Q000235:genetics* / Q000502:physiology; D064107:Aurora Kinase B / Q000235:genetics* / Q000502:physiology; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D000072379:NIMA-Related Kinases / Q000235:genetics / Q000502:physiology; D054462:p21-Activated Kinases / Q000235:genetics* / Q000502:physiology
chemical_list: D064096:Aurora Kinase A; D064107:Aurora Kinase B; C459456:NEK6 protein, human; D000072379:NIMA-Related Kinases; C515903:PAK1 protein, human; D054462:p21-Activated Kinases
keywords: AURKA; AURKB; Colorectal adenocarcinoma; Colorectal adenomatous polyp; Epigenetic; Histone modification; NEK6; PAK1