title: NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro.
abstract: The in vitro inter- and intra-cellular distribution of an isotopically labelled ruthenium(II)-arene (RAPTA) anti-metastatic compound in human ovarian cancer cells was imaged using nano-scale secondary ion mass spectrometry (NanoSIMS). Ultra-high resolution isotopic images of (13)C, (15)N, and Ru indicate that the phosphine ligand remains coordinated to the ruthenium(II) ion whereas the arene detaches. The complex localizes mainly on the membrane or at the interface between cells which correlates with its anti-metastatic effects.
authors.affiliation: Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. paul.dyson@epfl.ch.
authors.forename: Ronald F S
authors.initials: RF
authors.lastname: Lee
authors.affiliation: Laboratory for Biological Geochemistry, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. anders.meibom@epfl.ch.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Escrig
authors.affiliation: Interdisciplinary Centre for Electron Microscopy, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. graham.knott@epfl.ch.
authors.forename: Marie
authors.initials: M
authors.lastname: Croisier
authors.affiliation: Interdisciplinary Centre for Electron Microscopy, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. graham.knott@epfl.ch.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Clerc-Rosset
authors.affiliation: Interdisciplinary Centre for Electron Microscopy, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. graham.knott@epfl.ch.
authors.forename: Graham W
authors.initials: GW
authors.lastname: Knott
authors.affiliation: Laboratory for Biological Geochemistry, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. anders.meibom@epfl.ch and Center for Advanced Surface Analysis, Institute of Earth Sciences, University of Lausanne, CH-1015 Lausanne, Switzerland.
authors.forename: Anders
authors.initials: A
authors.lastname: Meibom
authors.affiliation: Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Curt A
authors.initials: CA
authors.lastname: Davey
authors.affiliation: Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. paul.dyson@epfl.ch.
authors.forename: Kai
authors.initials: K
authors.lastname: Johnsson
authors.affiliation: Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. paul.dyson@epfl.ch.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Dyson