title: Complete genome sequence of a novel endornavirus isolated from hot pepper.
abstract: The complete genome of a putative new endornavirus infecting hot peppers (Capsicum annuum) was determined to be 14,729 nt in size, including 12 cytosines at the 3' end. The hot pepper-infecting virus has the highest nucleotide sequence similarity (94% query cover and 72% identity) to bell pepper endornavirus (BPEV) isolated from the cultivar Yolo Wonder in the USA (GenBank accession no. JN019858). The putative single, large open reading frame encodes a 4,884-amino-acid-long polyprotein that contains four putative functional domains: a viral methyltransferase, a viral RNA helicase, a glycosyltransferase, and an RNA-dependent RNA polymerase. A phylogenetic tree based on whole polyprotein sequences confirmed the close evolutionary relationship of the studied endornavirus to BPEV. The hot pepper-infecting virus also has a nick at nt position 975. Taken together, these results suggest that this virus belongs to a new species in the genus Endornavirus (family Endornaviridae), for which the name hot pepper endornavirus (HPEV) is proposed.
authors.affiliation: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea.|Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, Republic of Korea.
authors.forename: Seungmo
authors.initials: S
authors.lastname: Lim
authors.affiliation: National Institute of Crop Science, Rural Development Administration, Suwon, 441-707, Republic of Korea.
authors.forename: Kil Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea.|Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, Republic of Korea.
authors.forename: Fumei
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea.|Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, Republic of Korea.
authors.forename: Ran Hee
authors.initials: RH
authors.lastname: Yoo
authors.affiliation: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea.|Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, Republic of Korea.
authors.forename: Davaajargal
authors.initials: D
authors.lastname: Igori
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Republic of Korea. suheon@knu.ac.kr.|Institute of Plant Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea. suheon@knu.ac.kr.
authors.forename: Su-Heon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea. jsmoon@kribb.re.kr.|Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, Republic of Korea. jsmoon@kribb.re.kr.
authors.forename: Jae Sun
authors.identifier: http://orcid.org/0000-0003-0934-1533
authors.initials: JS
authors.lastname: Moon
mesh_terms: D001483:Base Sequence; D002212:Capsicum / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*