title: The compromise of dynamic disulfide/thiol homeostasis as a biomarker of oxidative stress in trichloroethylene exposure.
abstract: In this study, we aimed to investigate disulfide/thiol homeostasis in trichloroethylene (TCE) exposure. The study was carried out in 30 nonsmoker TCE-exposed workers with a variety of occupations. Additionally, 30 healthy nonsmoker volunteers were recruited as the control group. TCE exposure was determined by measuring urinary trichloroacetic acid (TCA) concentration. Median urinary TCA levels of exposed workers (20.5 mg/L) were significantly higher than control subjects (5 mg/L). Thiol and disulfide concentrations were determined using a novel automated method. Disulfide/thiol ratio was significantly higher in the exposed group (p < 0.001). Thiol/disulfide homeostasis was found to be disturbed in TCE-exposed workers. We predict that in TCE-exposed workers this disturbance can be a therapeutic target, and the efficiency of the treatment can easily be monitored by the novel method we used.
authors.affiliation: Department of Biochemistry, Occupational Diseases Hospital, Ankara, Turkey ceylandemirbal@gmail.com.
authors.forename: C
authors.initials: C
authors.lastname: Bal
authors.affiliation: Department of Pharmacology, Occupational Diseases Hospital, Ankara, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Büyükşekerci
authors.affiliation: Department of Biochemistry, Atatürk Educational and Research Hospital, Ankara, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Koca
authors.affiliation: Department of Pharmacology, Occupational Diseases Hospital, Ankara, Turkey.
authors.forename: E R
authors.initials: ER
authors.lastname: Ağış
authors.affiliation: Department of Biochemistry, Atatürk Educational and Research Hospital, Ankara, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Erdoğan
authors.affiliation: Department of Biochemistry, Atatürk Educational and Research Hospital, Ankara, Turkey.
authors.forename: P
authors.initials: P
authors.lastname: Baran
authors.affiliation: Department of Family Medicine, Occupational Diseases Hospital, Ankara, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Gündüzöz
authors.affiliation: Department of Public Health, Yıldırım Beyazıt University, Ankara, Turkey.
authors.forename: Öh
authors.initials: Ö
authors.lastname: Yilmaz
mesh_terms: D000395:Air Pollutants, Occupational / Q000633:toxicity* / Q000652:urine; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004220:Disulfides / Q000097:blood*; D006706:Homeostasis / Q000187:drug effects*; D006801:Humans; D008297:Male; D016273:Occupational Exposure / Q000009:adverse effects* / Q000032:analysis; D013438:Sulfhydryl Compounds / Q000097:blood*; D014241:Trichloroethylene / Q000633:toxicity* / Q000652:urine
chemical_list: D000395:Air Pollutants, Occupational; D015415:Biomarkers; D004220:Disulfides; D013438:Sulfhydryl Compounds; D014241:Trichloroethylene
keywords: Trichloroethylene; disulfide; exposure; oxidative stress; thiol