title: Notch2 signaling contributes to cell growth, invasion, and migration in salivary adenoid cystic carcinoma.
abstract: Many studies have explored whether the Notch signaling pathway has a tumor-suppressive or an oncogenic role in various tumors; however, the role of the Notch signaling pathway in salivary adenoid cystic carcinoma (SACC) is still unknown. In this study, we attempt to define the role of Notch2 signaling in cell growth, invasion, and migration in SACC. We compared Notch2 expression in clinical SACC samples with that of normal samples by using immunohistochemical staining. Then, we down-regulated Notch2 expression to observe the effect of Notch2 on proliferation, invasion, migration, and the expression of known target genes of Notch signal pathway. According to our results, Notch2 expression was higher in SACC tissues compared with normal tissues. Knockdown of Notch2 inhibited cell proliferation, invasion, and migration in vitro and down-regulated the expression of HEY2 and CCND1. The results of this study suggest that Notch2 has an essential role in the cell growth, invasion, and migration of SACC. Notch2 may therefore be a potential target gene for the treatment of SACC by interfering with cell growth and metastasis.
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, 350002, Fujian, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qu
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, 350002, Fujian, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, 350002, Fujian, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, 350002, Fujian, People's Republic of China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Huang
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, 350002, Fujian, People's Republic of China.
authors.forename: Xiao-Meng
authors.initials: XM
authors.lastname: Hu
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, 350002, Fujian, People's Republic of China.
authors.forename: Rui-Huan
authors.initials: RH
authors.lastname: Gan
authors.affiliation: Department of Pathology, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, 350002, Fujian, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Oral and Maxillofacial Surgery, Affiliated First Hospital of Fujian Medical University, Fuzhou, 350005, Fujian, People's Republic of China.
authors.forename: Li-Song
authors.initials: LS
authors.lastname: Lin
authors.affiliation: Center of Dental Implant, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, 350002, Fujian, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350108, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Lin
authors.affiliation: Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350108, People's Republic of China. dalizheng@mail.fjmu.edu.cn.
authors.forename: Da-Li
authors.initials: DL
authors.lastname: Zheng
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, 350002, Fujian, People's Republic of China. fjlyg63@163.com.
authors.forename: You-Guang
authors.initials: YG
authors.lastname: Lu
mesh_terms: D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D055785:Gene Knockdown Techniques; D006801:Humans; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D051883:Receptor, Notch2 / Q000235:genetics / Q000378:metabolism*; D012468:Salivary Gland Neoplasms / Q000378:metabolism / Q000473:pathology*; D015398:Signal Transduction
chemical_list: C497764:NOTCH2 protein, human; D051883:Receptor, Notch2
keywords: Invasion; Migration; Notch2; Proliferation; Salivary adenoid cystic carcinoma