title: Protective Effects of UCF-101 on Cerebral Ischemia-Reperfusion (CIR) is Depended on the MAPK/p38/ERK Signaling Pathway.
abstract: This study was aimed to investigate the treatment mechanisms of 5-[5-(2-nitrophenyl) furfuryliodine]-1,3-diphenyl-2-thiobarbituric acid (UCF-101) in cerebral ischemia-reperfusion (CIR) model rats. Total of 54 healthy male Wistar rats were randomly assigned into three groups, namely sham group, vehicle group, and UCF-101 group. The CIR-injured model was established by right middle cerebral artery occlusion and reperfusion. Neurological function was assessed by an investigator according to the Longa neurologic deficit scores. Meanwhile, the cerebral tissue morphology and apoptotic neurons were evaluated by H&E and TUNEL staining, respectively. Additionally, the expressions of caspase 3, p-p38, and p-ERK were detected by immunohistochemistry or/and Western blotting assays. As results, neurologic deficit and pathological damage were obviously enhanced and TUNEL positive neurons were significantly increased in CIR-injured rats, as compared with those in sham group. Furthermore, the expressions of caspase 3, p-p38, and p-ERK were also significantly increased in vehicle group than those in sham group (P < 0.05). However, UCF-101 treatment could markedly weaken the neurologic deficit with lower scores and improve pathological condition. After UCF-101 treatment, TUNEL positive neurons as well as the expression of caspase 3 were significantly decreased than those in vehicle group (P < 0.05). Besides, p-p38 was decreased while p-ERK was increased in UCF-101 group than those in vehicle group (P < 0.05). Therefore, we concluded that the protective effects of UCF-101 might be associated with apoptosis process and MAPK signaling pathway in the CIR-injured model.
authors.affiliation: Department of Neurology, The Fourth Affiliated Hospital of Harbin Medical University, 150000, Harbin, People's Republic of China.
authors.forename: Danying
authors.initials: D
authors.lastname: Su
authors.affiliation: Department of Anatomy, Harbin Medical University, 150000, Harbin, People's Republic of China. Jingma2009@aliyun.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Neurology, The Fourth Affiliated Hospital of Harbin Medical University, 150000, Harbin, People's Republic of China.
authors.forename: Zhuobo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, 150000, Harbin, People's Republic of China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Imaging, The Fourth Affiliated Hospital Harbin Medical University, No. 37 Yiyuan Str, Nangang District, 150001, Harbin, People's Republic of China. baozhong_shen@163.com.
authors.forename: Baozhong
authors.initials: B
authors.lastname: Shen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002545:Brain Ischemia / Q000188:drug therapy* / Q000473:pathology*; D004195:Disease Models, Animal; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008297:Male; D011744:Pyrimidinones / Q000008:administration & dosage / Q000494:pharmacology*; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology; D013871:Thiones / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D011744:Pyrimidinones; D013871:Thiones; C501517:UCF 101
keywords: Apoptosis neurons; Cerebral ischemia–reperfusion; MAPK; UCF-101