title: Development and characterization of 11 microsatellite primers for the sedge Trichophorum planifolium (Cyperaceae).
abstract: OBJECTIVE
  Microsatellite loci were developed for Trichophorum planifolium (Cyperaceae), an endangered woodland sedge protected under federal and provincial legislation in Canada, to explore patterns of population genetic diversity and differentiation in the species.
  
  
  RESULTS
  Sixty-three primer pairs were evaluated for amplification consistency and screened for polymorphisms in 96 samples collected from 12 populations of T. planifolium distributed through the range of the species. Of these, 11 loci were shown to be polymorphic, displaying two to six alleles. Mean observed heterozygosity across loci ranged from 0.00 to 0.06 among populations tested.
  
  
  CONCLUSIONS
  The results suggest that the 11 primer pairs developed in this study will be useful for future studies of broad-scale genetic variation in T. planifolium and in guiding management protocols for the species in Canada.
authors.affiliation: Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada.
authors.forename: Victoria J
authors.initials: VJ
authors.lastname: Nowell
authors.affiliation: Agriculture and Agri-Food Canada (AAFC), 960 Carling Avenue, K.W. Neatby Building, Ottawa, Ontario K1A 0C6, Canada.
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada ; Agriculture and Agri-Food Canada (AAFC), 960 Carling Avenue, K.W. Neatby Building, Ottawa, Ontario K1A 0C6, Canada.
authors.forename: Tyler W
authors.initials: TW
authors.lastname: Smith
keywords: Cyperaceae; Trichophorum planifolium; conservation genetics; microsatellites