title: Oxidative status in treatment-naïve essential thrombocythemia: a pilot study in a single center.
abstract: Oxidative stress (OS), due to pro-oxidant species [reactive oxygen species (ROS)] excess not counterbalanced by endogenous antioxidant molecules [e.g., reduced glutathione (GSH)], is involved in the pathogenesis of human cancers, but few data are available on essential thrombocythemia (ET). This study aims to investigate OS in ET off-therapy patients. Thirty ET treatment-naïve patients were compared with 26 age-matched and gender-matched controls. Serum ROS, urinary 8-hydroxydeoxyguanosine, full blood GSH levels, and reduced/oxidized GSH ratio (GSH/GSSG) were measured. Data were adjusted for gender, age, JAK2 mutational status, smoking, dyslipidemia, or hypercholesterolemia requiring drug therapy, antiplatelet therapy, treatment with acetylsalicylic acid, high-sensitive C-reactive protein levels, and absolute monocyte count. ROS and GSH levels were increased in both patients and controls. Patients showed increased GSSG (p = 0.05), reduced GSH/GSSG ratio (p = 0.08), and similar 8-hydroxydeoxyguanosine levels when compared with controls. No differences in OS parameters were found between JAK2-positive and JAK2-negative patients. Confounding factors did not modify the results. Our study suggests an OS condition in a cohort of treatment-naïve ET patients, not associated with JAK2 mutational status or with chronic inflammation situation. GSH/GSSG ratio, altered in ET patients because of increased GSSG levels, showed the presence of higher GSH levels in ET than controls as a possible compensatory mechanism of an excess of pro-oxidant production. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Oncohematology Division, IRCCS Ca' Granda-Maggiore Policlinico Hospital Foundation, University of Milan, Milano, Italy.|Oncohematology Unit of the Elderly, IRCCS Ca' Granda-Maggiore Policlinico Hospital Foundation, Milano, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Iurlo
authors.affiliation: Department of Hematology-Oncology and BMT Unit, IRCCS Ca' Granda-Maggiore Policlinico Hospital Foundation, Department of Biomedical, Surgical, and Dental Sciences, University of Milan, Milano, Italy.
authors.forename: Rachele
authors.initials: R
authors.lastname: De Giuseppe
authors.affiliation: Oncohematology Division, IRCCS Ca' Granda-Maggiore Policlinico Hospital Foundation, University of Milan, Milano, Italy.
authors.forename: Mariarita
authors.initials: M
authors.lastname: Sciumè
authors.affiliation: Oncohematology Division, IRCCS Ca' Granda-Maggiore Policlinico Hospital Foundation, University of Milan, Milano, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Cattaneo
authors.affiliation: Oncohematology Division, IRCCS Ca' Granda-Maggiore Policlinico Hospital Foundation, University of Milan, Milano, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Fermo
authors.affiliation: Department of Hematology-Oncology and BMT Unit, IRCCS Ca' Granda-Maggiore Policlinico Hospital Foundation, Department of Biomedical, Surgical, and Dental Sciences, University of Milan, Milano, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: De Vita
authors.affiliation: Epidemiology Unit, Department of Preventive Medicine, IRCCS Ca' Granda-Maggiore Policlinico Hospital Foundation, Milano, Italy.
authors.forename: Dario
authors.initials: D
authors.lastname: Consonni
authors.affiliation: Laboratory of Clinical Chemistry and Microbiology, IRCCS Ca' Granda-Maggiore Policlinico Hospital Foundation, Milano, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Maiavacca
authors.affiliation: Department of Hematology-Oncology and BMT Unit, IRCCS Ca' Granda-Maggiore Policlinico Hospital Foundation, Department of Biomedical, Surgical, and Dental Sciences, University of Milan, Milano, Italy.
authors.forename: Fabrizia
authors.initials: F
authors.lastname: Bamonti
authors.affiliation: Hematopathology Service, Division of Pathology, Department of Pathophysiology and Transplantation, IRCCS Ca' Granda-Maggiore Policlinico Hospital Foundation, University of Milan, Milano, Italy.
authors.forename: Umberto
authors.initials: U
authors.lastname: Gianelli
authors.affiliation: Oncohematology Division, IRCCS Ca' Granda-Maggiore Policlinico Hospital Foundation, University of Milan, Milano, Italy.
authors.forename: Agostino
authors.initials: A
authors.lastname: Cortelezzi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D015415:Biomarkers; D001706:Biopsy; D001856:Bone Marrow Examination; D016022:Case-Control Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010865:Pilot Projects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012307:Risk Factors; D013920:Thrombocythemia, Essential / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D053614:Janus Kinase 2; D003849:Deoxyguanosine; D005978:Glutathione
keywords: essential thrombocythemia; glutathione; oxidative stress; reactive oxygen species