title: Molecular epidemiological analyses of Cryptosporidium parvum virus 1 (CSpV1), a symbiotic virus of Cryptosporidium parvum, in Japan.
abstract: We show that Cryptosporidium parvum virus 1 (CSpV1), a member of the family Partitiviridae, genus Cryspovirus that can infect Cryptosporidium parvum, is a new candidate for high-resolution tool for tracing C. parvum. CSpV1 was detected in all C. parvum-positive samples tested. Phylogenetic analysis of dsRNA1 sequence from CSpV1 can distinguish infected areas of C. parvum on the national level. Sequences detected in samples from Iwate prefecture and other islands (Tanegashima, and Okinawa) belonged to a single clade. This system can differentiate the samples from Hokkaido and south part of Japan as well as from other countries. Samples from Iwate, Tanegashima, and Okinawa belonged to a single subclade, respectively. Therefore, the CSpV1 dsRNA sequences reflect the regional distribution of their host and have potential as a high-resolution tool to trace C. parvum IIaA15G2R1 subtype.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan; Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Fumi
authors.initials: F
authors.lastname: Murakoshi
authors.affiliation: Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
authors.forename: Madoka
authors.initials: M
authors.lastname: Ichikawa-Seki
authors.affiliation: Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
authors.forename: Junya
authors.initials: J
authors.lastname: Aita
authors.affiliation: Kumage Agricultural Mutual Aid Association, 6410-8 Noma, Nakatane-cho, Kumage, Kagoshima 891-3604, Japan.
authors.forename: Seiko
authors.initials: S
authors.lastname: Yaita
authors.affiliation: Veterinarian of Iwate Prefecture, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Kinami
authors.affiliation: Veterinary Clinical Center, Ishikari Agricultural Mutual Aid Association, 7-4-1 Seiryu, Chitose, Hokkaido 066-0081, Japan.
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Fujimoto
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Nishikawa
authors.affiliation: Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Murakami
authors.affiliation: Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Horimoto
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan; Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address: kkato@obihiro.ac.jp.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kato
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000502:physiology / Q000821:virology*; D005243:Feces / Q000469:parasitology; D005838:Genotype; D007564:Japan / Q000453:epidemiology; D010802:Phylogeny; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D013559:Symbiosis
keywords: Cryprosporidium parvum; Cryptosporidium parvum virus 1; Epidemiology; Partitiviridae; dsRNA viruses