title: Molecular Analysis of IGH and Incomplete IGH D-J Clonality Gene Rearrangements in Hodgkin Lymphoma Malignancies.
abstract: BACKGROUND
  We evaluated molecular clonality in immunoglobulin heavy chain (IGH) and incomplete IGH D-J genes for improvement of clinical diagnosis of Hodgkin's lymphoma (HL). We applied BIOMED-2 protocols in HL cases, which were previously approved by clonality detection in non-Hodgkin lymphoma (NHL) cases.
  
  
  METHODS
  We investigated 50 consecutive FFPE samples of classical HL (cHL) patients to assess IGH and IGH D-J clonal gene rearrangements by multiplex PCR protocols, which were provided by the European Biomedicine and Health (BIOMED-2) Concerted Action Project BMH4-CT98-3936.
  
  
  RESULTS
  In the present study, there was a monoclonality of 86% (43/50) including a clonality of 74% (37/50) for IGH and a clonality of 42% (21/50) in IGHD-J. In addition, a lack of clonality was detected in 14% (7/50) of cases. Frequent gene rearrangements were detected in framework (FR) III (54%) and FRII (20%), whereas no clonality was seen in FRI. Furthermore, a monoclonality of 28% and 14% was detected in the DH(1-6)-JH and DH(see symbol)-JH gene rearrangements, respectively.
  
  
  CONCLUSIONS
  The present study suggests that the complete IGH and incomplete IGH D-J clonality gene rearrangement assays using BIOMED-2 protocols could be considered a valuable method for detection of clonal gene rearrangements, especially in HL cases.
authors.forename: Saeid
authors.initials: S
authors.lastname: Ghorbian
authors.forename: Issa
authors.initials: I
authors.lastname: Jahanzad
authors.forename: Mehrdad Asghari
authors.initials: MA
authors.lastname: Estiar
authors.forename: Jamal Eivazi
authors.initials: JE
authors.lastname: Ziae
authors.forename: Iraj
authors.initials: I
authors.lastname: Asvadi-Kermani
authors.forename: Sasan
authors.initials: S
authors.lastname: Andalib
authors.forename: Gholam Reza
authors.initials: GR
authors.lastname: Javadi
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Sakhinia
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D019295:Computational Biology; D015972:Gene Expression Regulation, Neoplastic; D015321:Gene Rearrangement; D050438:Genes, Immunoglobulin Heavy Chain; D005820:Genetic Testing / Q000379:methods*; D006689:Hodgkin Disease / Q000235:genetics* / Q000276:immunology; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction; D011237:Predictive Value of Tests
chemical_list: D014408:Biomarkers, Tumor