title: Transcriptional over-expression of chloride intracellular channels 3 and 4 in malignant pleural mesothelioma.
abstract: BACKGROUND
  Chloride Intracellular Channels (CLICs) are contributing to the regulation of multiple cellular functions. CLICs have been found over-expressed in several malignancies, and therefore they are currently considered as potential drug targets. The goal of our study was to assess the gene expression levels of the CLIC's 1-6 in malignant pleural mesothelioma (MPM) as compared to controls.
  
  
  METHODS
  We used gene expression data from a publicly available microarray dataset comparing MPM versus healthy tissue in order to investigate the differential expression profile of CLIC 1-6. False discovery rates were calculated and the interactome of the significantly differentially expressed CLICs was constructed and Functional Enrichment Analysis for Gene Ontologies (FEAGO) was performed.
  
  
  RESULTS
  In MPM, the gene expressions of CLIC3 and CLIC4 were significantly increased compared to controls (p=0.001 and p<0.001 respectively). A significant positive correlation between the gene expressions of CLIC3 and CLIC4 (p=0.0008 and Pearson's r=0.51) was found. Deming regression analysis provided an association equation between the CLIC3 and CLIC4 gene expressions: CLIC3=4.42CLIC4-10.07.
  
  
  CONCLUSIONS
  Our results indicate that CLIC3 and CLIC4 are over-expressed in human MPM. Moreover, their expressions correlate suggesting that they either share common gene expression inducers or that their products act synergistically. FAEGO showed that CLIC interactome might contribute to TGF beta signaling and water transport.
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa 41500, Greece.
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Tasiopoulou
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa 41500, Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Magouliotis
authors.affiliation: Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russia; Novosibirsk State University, Novosibirsk 630090, Russia.
authors.forename: Evgeniy I
authors.initials: EI
authors.lastname: Solenov
authors.affiliation: Department of Respiratory Medicine, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa 41500, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Vavougios
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa 41500, Greece.
authors.forename: Paschalis-Adam
authors.initials: PA
authors.lastname: Molyvdas
authors.affiliation: Department of Respiratory Medicine, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa 41500, Greece.
authors.forename: Konstantinos I
authors.initials: KI
authors.lastname: Gourgoulianis
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa 41500, Greece; Department of Respiratory Medicine, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa 41500, Greece.
authors.forename: Chrissi
authors.initials: C
authors.lastname: Hatzoglou
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa 41500, Greece; Department of Respiratory Medicine, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa 41500, Greece. Electronic address: szarog@med.uth.gr.
authors.forename: Sotirios G
authors.initials: SG
authors.lastname: Zarogiannis
mesh_terms: D018118:Chloride Channels / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics*; D008654:Mesothelioma / Q000175:diagnosis / Q000235:genetics*; D000086002:Mesothelioma, Malignant; D010997:Pleural Neoplasms / Q000175:diagnosis / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: C117019:CLIC3 protein, human; C464459:CLIC4 protein, human; D018118:Chloride Channels
keywords: Chloride; Gene expression; Ion Channel; Mesothelioma; Transcitpromics