title: Microbioreactors for Cartilage Tissue Engineering.
abstract: In tissue engineering research, cell-based assays are widely utilized to fundamentally explore cellular responses to extracellular conditions. Nevertheless, the simplified cell culture models available at present have several inherent shortcomings and limitations. To tackle the issues, a wide variety of microbioreactors for cell culture have been actively proposed, especially during the past decade. Among these, micro-scale cell culture devices based on microfluidic biochip technology have particularly attracted considerable attention. In this chapter, we not only discuss the advantageous features of using micro-scale cell culture devices for cell-based assays, but also describe their fabrication, experimental setup, and application.
authors.affiliation: Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, Linko, Taiwan.
authors.forename: Yu-Han
authors.initials: YH
authors.lastname: Chang
authors.affiliation: Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan. mhwu@mail.cgu.edu.tw.
authors.forename: Min-Hsien
authors.initials: MH
authors.lastname: Wu
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D019149:Bioreactors; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D019902:Chondrocytes / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D020219:Chondrogenesis; D004867:Equipment Design; D006801:Humans; D056656:Lab-On-A-Chip Devices; D040542:Mechanotransduction, Cellular; D008904:Miniaturization; D010812:Physical Stimulation; D012038:Regeneration; D044968:Regenerative Medicine / Q000295:instrumentation* / Q000379:methods; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds
keywords: Cell-based assays; Microbioreactors; Microfluidic biochip; Perfusion cell culture; Tissue engineering