title: Shear and Compression Bioreactor for Cartilage Synthesis.
abstract: Mechanical forces, including hydrodynamic shear, hydrostatic pressure, compression, tension, and friction, can have stimulatory effects on cartilage synthesis in tissue engineering systems. Bioreactors capable of exerting forces on cells and tissue constructs within a controlled culture environment are needed to provide appropriate mechanical stimuli. In this chapter, we describe the construction, assembly, and operation of a mechanobioreactor providing simultaneous dynamic shear and compressive loading on developing cartilage tissues to mimic the rolling and squeezing action of articular joints. The device is suitable for studying the effects of mechanical treatment on stem cells and chondrocytes seeded into three-dimensional scaffolds.
authors.affiliation: Westmead Millennium Institute for Medical Research, University of Sydney, Westmead, NSW, 2145, Australia. kifah.shahin@health.nsw.gov.au.
authors.forename: Kifah
authors.initials: K
authors.lastname: Shahin
authors.affiliation: Faculty of Science, Engineering and Technology, Swinburne University of Technology, P.O. Box 218, Hawthorn, Melbourne, VIC, 3122, Australia.
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Doran
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D019149:Bioreactors; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D019902:Chondrocytes / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D020219:Chondrogenesis; D004867:Equipment Design; D006801:Humans; D040542:Mechanotransduction, Cellular; D010812:Physical Stimulation; D012038:Regeneration; D044968:Regenerative Medicine / Q000295:instrumentation* / Q000379:methods; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds
keywords: Cyclical shear force; Dynamic compression; Mechanical loading; Mechanobioreactor; Simultaneous shear and compression; Synovial joint