title: Ferromagnetic Bare Metal Stent for Endothelial Cell Capture and Retention.
abstract: Rapid endothelialization of cardiovascular stents is needed to reduce stent thrombosis and to avoid anti-platelet therapy which can reduce bleeding risk. The feasibility of using magnetic forces to capture and retain endothelial outgrowth cells (EOC) labeled with super paramagnetic iron oxide nanoparticles (SPION) has been shown previously. But this technique requires the development of a mechanically functional stent from a magnetic and biocompatible material followed by in-vitro and in-vivo testing to prove rapid endothelialization. We developed a weakly ferromagnetic stent from 2205 duplex stainless steel using computer aided design (CAD) and its design was further refined using finite element analysis (FEA). The final design of the stent exhibited a principal strain below the fracture limit of the material during mechanical crimping and expansion. One hundred stents were manufactured and a subset of them was used for mechanical testing, retained magnetic field measurements, in-vitro cell capture studies, and in-vivo implantation studies. Ten stents were tested for deployment to verify if they sustained crimping and expansion cycle without failure. Another 10 stents were magnetized using a strong neodymium magnet and their retained magnetic field was measured. The stents showed that the retained magnetism was sufficient to capture SPION-labeled EOC in our in-vitro studies. SPION-labeled EOC capture and retention was verified in large animal models by implanting 1 magnetized stent and 1 non-magnetized control stent in each of 4 pigs. The stented arteries were explanted after 7 days and analyzed histologically. The weakly magnetic stents developed in this study were capable of attracting and retaining SPION-labeled endothelial cells which can promote rapid healing.
authors.affiliation: Division of Engineering, Mayo Clinic.
authors.forename: Susheil
authors.initials: S
authors.lastname: Uthamaraj
authors.affiliation: Division of Cardiovascular Diseases, Mayo Clinic.
authors.forename: Brandon J
authors.initials: BJ
authors.lastname: Tefft
authors.affiliation: Department of Cardioangiology, ICRC, St. Anne's University Hospital.
authors.forename: Ota
authors.initials: O
authors.lastname: Hlinomaz
authors.affiliation: Division of Cardiovascular Diseases, Mayo Clinic.
authors.forename: Gurpreet S
authors.initials: GS
authors.lastname: Sandhu
authors.affiliation: Division of Engineering, Mayo Clinic; Mayo Clinic College of Medicine; dragomirdaescu.dan@mayo.edu.
authors.forename: Dan
authors.initials: D
authors.lastname: Dragomir-Daescu
mesh_terms: D000818:Animals; D017076:Computer-Aided Design; D003584:Cytological Techniques / Q000295:instrumentation* / Q000379:methods; D042783:Endothelial Cells / Q000166:cytology*; D004867:Equipment Design; D020342:Finite Element Analysis; D008280:Magnetics / Q000295:instrumentation / Q000379:methods; D058185:Magnetite Nanoparticles; D059346:Magnets; D015607:Stents; D013552:Swine
chemical_list: D058185:Magnetite Nanoparticles