title: Identification of the centromeric repeat in the threespine stickleback fish (Gasterosteus aculeatus).
abstract: Centromere sequences exist as gaps in many genome assemblies due to their repetitive nature. Here we take an unbiased approach utilizing centromere protein A (CENP-A) chomatin immunoprecipitation followed by high-throughput sequencing to identify the centromeric repeat sequence in the threespine stickleback fish (Gasterosteus aculeatus). A 186-bp, AT-rich repeat was validated as centromeric using both fluorescence in situ hybridization (FISH) and immunofluorescence combined with FISH (IF-FISH) on interphase nuclei and metaphase spreads. This repeat hybridizes strongly to the centromere on all chromosomes, with the exception of weak hybridization to the Y chromosome. Together, our work provides the first validated sequence information for the threespine stickleback centromere.
authors.affiliation: Divisions of Human Biology and Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave North, Mailstop C2-023, Seattle, WA, 98109, USA.|Graduate Program in Molecular and Cellular Biology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Jennifer N
authors.initials: JN
authors.lastname: Cech
authors.affiliation: Divisions of Human Biology and Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave North, Mailstop C2-023, Seattle, WA, 98109, USA. cpeichel@fhcrc.org.
authors.forename: Catherine L
authors.initials: CL
authors.lastname: Peichel
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D002503:Centromere / Q000235:genetics*; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005260:Female; D005399:Fishes / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011485:Protein Binding; D016415:Sequence Alignment; D020080:Tandem Repeat Sequences; D014998:Y Chromosome
chemical_list: D001324:Autoantigens; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone
keywords: CENP-A; Centromere; ChIP-seq; Gasterosteus aculeatus; Threespine stickleback