title: Ultratrace DNA Detection Based on the Condensing-Enrichment Effect of Superwettable Microchips.
abstract: A sensitive nucleic acid detection platform based on superhydrophilic microwells spotted on a superhydrophobic substrate is fabricated. Due to the wettability differences, ultratrace DNA molecules are enriched and the fluorescent signals are amplified to allow more sensitive detection. The biosensing interface based on superwettable materials provides a simple and cost-effective way for ultratrace DNA sensing.
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing, 100083, P. R. China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Xu
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing, 100083, P. R. China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Laboratory of Bioinspired Smart Interface Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
authors.forename: Gao
authors.initials: G
authors.lastname: Yang
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing, 100083, P. R. China.
authors.forename: Wanxin
authors.initials: W
authors.lastname: Shi
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing, 100083, P. R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Dai
authors.affiliation: Laboratory of Bioinspired Smart Interface Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
authors.forename: Guannan
authors.initials: G
authors.lastname: Li
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing, 100083, P. R. China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing, 100083, P. R. China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing, 100083, P. R. China.
authors.forename: Xueji
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Laboratory of Bioinspired Smart Interface Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
authors.forename: Shutao
authors.initials: S
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D057230:Limit of Detection; D046208:Microchip Analytical Procedures / Q000379:methods*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D017155:Wettability
chemical_list: D004247:DNA
keywords: DNA detection; condensing enrichment; microchips; superhydrophilic; superhydrophobic; ultratrace analysis