title: Pancreatic amylase is an environmental signal for regulation of biofilm formation and host interaction in Campylobacter jejuni.
abstract: Campylobacter jejuni is a commensal bacterium in the intestines of animals and birds and a major cause of food-borne gastroenteritis in humans worldwide. Here we show that exposure to pancreatic amylase leads to secretion of an α-dextran by C. jejuni and that a secreted protease, Cj0511, is required. Exposure of C. jejuni to pancreatic amylase promotes biofilm formation in vitro, increases interaction with human epithelial cell lines, increases virulence in the Galleria mellonella infection model, and promotes colonization of the chicken ileum. We also show that exposure to pancreatic amylase protects C. jejuni from stress conditions in vitro, suggesting that the induced α-dextran may be important during transmission between hosts. This is the first evidence that pancreatic amylase functions as an interkingdom signal in an enteric microorganism.
authors.affiliation: Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, United Kingdom.
authors.forename: Waheed
authors.initials: W
authors.lastname: Jowiya
authors.affiliation: Department of Infection and Immunity, Institute of Child Health, University College London, London, United Kingdom.
authors.forename: Katja
authors.initials: K
authors.lastname: Brunner
authors.affiliation: Faculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom.
authors.forename: Sherif
authors.initials: S
authors.lastname: Abouelhadid
authors.affiliation: Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, United Kingdom.
authors.forename: Haitham A
authors.initials: HA
authors.lastname: Hussain
authors.affiliation: Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, United Kingdom.
authors.forename: Sean P
authors.initials: SP
authors.lastname: Nair
authors.affiliation: School of Applied Sciences, University of Huddersfield, Huddersfield, United Kingdom.
authors.forename: Sohaib
authors.initials: S
authors.lastname: Sadiq
authors.affiliation: School of Veterinary Sciences, University of Bristol, Bristol, United Kingdom.
authors.forename: Lisa K
authors.initials: LK
authors.lastname: Williams
authors.affiliation: School of Veterinary Sciences, University of Bristol, Bristol, United Kingdom.
authors.forename: Emma K
authors.initials: EK
authors.lastname: Trantham
authors.affiliation: Department of Infection and Immunity, Institute of Child Health, University College London, London, United Kingdom.
authors.forename: Holly
authors.initials: H
authors.lastname: Stephenson
authors.affiliation: Faculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom.
authors.forename: Brendan W
authors.initials: BW
authors.lastname: Wren
authors.affiliation: Department of Infection and Immunity, Institute of Child Health, University College London, London, United Kingdom.
authors.forename: Mona
authors.initials: M
authors.lastname: Bajaj-Elliott
authors.affiliation: School of Veterinary Sciences, University of Bristol, Bristol, United Kingdom.
authors.forename: Tristan A
authors.initials: TA
authors.lastname: Cogan
authors.affiliation: School of Applied Sciences, University of Huddersfield, Huddersfield, United Kingdom.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Laws
authors.affiliation: Medical Microbiology, King's College Hospital, London, United Kingdom.
authors.forename: Jim
authors.initials: J
authors.lastname: Wade
authors.affiliation: Faculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom.
authors.forename: Nick
authors.initials: N
authors.lastname: Dorrell
authors.affiliation: Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, United Kingdom e.allan@ucl.ac.uk.
authors.forename: Elaine
authors.initials: E
authors.lastname: Allan
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D018938:Caco-2 Cells; D002169:Campylobacter Infections / Q000201:enzymology / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology; D045744:Cell Line, Tumor; D002645:Chickens; D003911:Dextrans / Q000096:biosynthesis / Q000378:metabolism; D004847:Epithelial Cells; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D006801:Humans; D007422:Intestines / Q000382:microbiology / Q000473:pathology; D009036:Moths / Q000382:microbiology; D055292:Pancreatic alpha-Amylases / Q000302:isolation & purification / Q000494:pharmacology*; D010447:Peptide Hydrolases / Q000235:genetics* / Q000378:metabolism; D011201:Poultry Diseases / Q000201:enzymology / Q000382:microbiology* / Q000473:pathology; D015398:Signal Transduction; D013552:Swine
chemical_list: D001426:Bacterial Proteins; D003911:Dextrans; C040834:alpha-1,3-dextran; D055292:Pancreatic alpha-Amylases; D010447:Peptide Hydrolases