title: Simultaneous Optogenetic Stimulation of Individual Pharyngeal Neurons and Monitoring of Feeding Behavior in Intact C. elegans.
abstract: Optogenetic approaches have proven powerful for examining the role of neural circuits in generating behaviors, especially in systems where electrophysiological manipulation is not possible. Here we describe a method for optogenetically manipulating single pharyngeal neurons in intact C. elegans while monitoring pharyngeal behavior. This approach provides bidirectional and dynamic control of pharyngeal neural activity simultaneously with a behavioral readout and has allowed us to test hypotheses about the roles of individual pharyngeal neurons in regulating feeding behavior.
authors.affiliation: Department of Bioengineering, University of Pennsylvania, 210 S. 33rd Street, Philadelphia, PA, 19104, USA.|Department of Neuroscience, University of Pennsylvania, CRB 211, 415 Curie Boulevard, Philadelphia, PA, 19104, USA.
authors.forename: Nicholas F
authors.initials: NF
authors.lastname: Trojanowski
authors.affiliation: Department of Bioengineering, University of Pennsylvania, 210 S. 33rd Street, Philadelphia, PA, 19104, USA. fangyen@seas.upenn.edu.|Department of Neuroscience, University of Pennsylvania, CRB 211, 415 Curie Boulevard, Philadelphia, PA, 19104, USA. fangyen@seas.upenn.edu.
authors.forename: Christopher
authors.initials: C
authors.lastname: Fang-Yen
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D005247:Feeding Behavior; D009474:Neurons / Q000502:physiology*; D062308:Optogenetics
keywords: C. elegans; Feeding behavior; In vivo optogenetics; Optogenetics; Pharynx; Small circuits