title: In Vitro Selection of Cancer Cell-Specific Molecular Recognition Elements from Amino Acid Libraries.
abstract: Differential cell systematic evolution of ligands by exponential enrichment (SELEX) is an in vitro selection method for obtaining molecular recognition elements (MREs) that specifically bind to individual cell types with high affinity. MREs are selected from initial large libraries of different nucleic or amino acids. This review outlines the construction of peptide and antibody fragment libraries as well as their different host types. Common methods of selection are also reviewed. Additionally, examples of cancer cell MREs are discussed, as well as their potential applications.
authors.affiliation: Memorial Sloan Kettering Cancer Center, Molecular Pharmacology & Chemistry, 1275 York Avenue, New York, NY 10065, USA ; Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USA.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Williams
authors.affiliation: Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USA.
authors.forename: Letha J
authors.initials: LJ
authors.lastname: Sooter
mesh_terms: D000596:Amino Acids; D000818:Animals; D052157:Aptamers, Nucleotide; D061505:Cell Surface Display Techniques; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D009369:Neoplasms / Q000235:genetics* / Q000276:immunology*; D019151:Peptide Library; D052156:SELEX Aptamer Technique
chemical_list: D000596:Amino Acids; D052157:Aptamers, Nucleotide; D008024:Ligands; D019151:Peptide Library