title: Five-day stability of thawed plasma: solvent/detergent-treated plasma comparable with fresh-frozen plasma and plasma frozen within 24 hours.
abstract: BACKGROUND
  Plasma stored refrigerated for up to 5 days after thawing is common practice in many US hospitals. Therefore, clotting factor activities in fresh-frozen plasma (FFP), plasma frozen within 24 hours (PF24), and solvent/detergent-treated plasma (SDP), thawed and stored at 1 to 6°C for up to 5 days, were investigated.
  
  
  METHODS
  Five A, B, O, and AB units of FFP, PF24, and SDP were thawed and maintained for 5 days at 1 to 6°C. The activity of factor (F)V, FVII, FVIII, protein S (PS), and ADAMTS13 was determined in each unit at baseline and every 24 hours thereafter for 5 days.
  
  
  RESULTS
  After thaw, mean values of the variables tested were within the normal range in all three plasma products although, in SDP, FVIII activity was significantly lower (p = 0.0039). After 5 days of storage all factors significantly declined except for ADAMTS13 activity, which was stable. Mean FVIII and ADAMTS13 activity was comparable in all three plasma products and within the normal range, mean FV activity was significantly lower in FFP and PF24 (p<0.0001) compared to SDP, and mean FVII activity was significantly lower in PF24 (p<0.03) than in FFP or SDP. Mean PS activity was below the normal range in all three plasma products with the lowest values in SDP (p = 0.0001).
  
  
  CONCLUSIONS
  Over 5 days of refrigerated storage the changes in the measured coagulation factors in FFP, PF24, and SDP are comparable. Clinical follow-up is needed to assess whether slightly lower PS levels in SDP are clinically important.
authors.affiliation: Scientific & Medical Affairs, Octapharma, Hoboken, New Jersey.
authors.forename: Andrea
authors.initials: A
authors.lastname: Neisser-Svae
authors.affiliation: Clinical Research Biometrics, Octapharma, Vienna, Austria.
authors.forename: Laurenz
authors.initials: L
authors.lastname: Trawnicek
authors.affiliation: Research & Development, Octapharma, Vienna, Austria.
authors.forename: Andrea
authors.initials: A
authors.lastname: Heger
authors.affiliation: Scientific & Medical Affairs, Octapharma, Hoboken, New Jersey.
authors.forename: Trupti
authors.initials: T
authors.lastname: Mehta
authors.affiliation: Institute for Transfusion Medicine, Pittsburgh, Pennsylvania.
authors.forename: Darrell
authors.initials: D
authors.lastname: Triulzi
mesh_terms: D051722:ADAM Proteins / Q000737:chemistry / Q000378:metabolism; D000071120:ADAMTS13 Protein; D001793:Blood Preservation; D003902:Detergents / Q000737:chemistry*; D005167:Factor VII / Q000737:chemistry / Q000378:metabolism; D005169:Factor VIII / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D010949:Plasma; D017293:Protein S / Q000737:chemistry / Q000378:metabolism; D055550:Protein Stability; D012997:Solvents / Q000737:chemistry*; D013997:Time Factors
chemical_list: D003902:Detergents; D017293:Protein S; D012997:Solvents; C078147:F8 protein, human; D005167:Factor VII; D005169:Factor VIII; D051722:ADAM Proteins; D000071120:ADAMTS13 Protein; C099604:ADAMTS13 protein, human