title: Complete genome sequence of Geobacillus thermoglucosidasius C56-YS93, a novel biomass degrader isolated from obsidian hot spring in Yellowstone National Park.
abstract: Geobacillus thermoglucosidasius C56-YS93 was one of several thermophilic organisms isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA under permit from the National Park Service. Comparison of 16 S rRNA sequences confirmed the classification of the strain as a G. thermoglucosidasius species. The genome was sequenced, assembled, and annotated by the DOE Joint Genome Institute and deposited at the NCBI in December 2011 (CP002835). The genome of G. thermoglucosidasius C56-YS93 consists of one circular chromosome of 3,893,306 bp and two circular plasmids of 80,849 and 19,638 bp and an average G + C content of 43.93 %. G. thermoglucosidasius C56-YS93 possesses a xylan degradation cluster not found in the other G. thermoglucosidasius sequenced strains. This cluster appears to be related to the xylan degradation cluster found in G. stearothermophilus. G. thermoglucosidasius C56-YS93 possesses two plasmids not found in the other two strains. One plasmid contains a novel gene cluster coding for proteins involved in proline degradation and metabolism, the other contains a collection of mostly hypothetical proteins.
authors.affiliation: Lucigen Corporation, Middleton, WI USA ; Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, WI USA.
authors.forename: Phillip J
authors.initials: PJ
authors.lastname: Brumm
authors.affiliation: Oak Ridge National Laboratory, Oak Ridge, TN USA.
authors.forename: Miriam L
authors.initials: ML
authors.lastname: Land
authors.affiliation: Lucigen Corporation, Middleton, WI USA ; Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, WI USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Mead
keywords: Biomass; Geobacillus thermoglucosidasius C56-YS93; Hot springs; Prophage; Xylan