title: Identification of six new RHCE variant alleles in individuals of diverse racial origin.
abstract: BACKGROUND
  The introduction of molecular methods into routine blood typing is prompting the identification of new blood group alleles. Discrepancies between the results of genotyping and serology or chance events uncovered during genotyping prompted additional investigations, which revealed six new RHCE variant alleles.
  
  
  METHODS
  Samples from eight blood donors, two patients (one prenatal), and a patient's relative, all of diverse racial origin, were analyzed by standard serology methods, targeted genotyping arrays, DNA sequencing, and allele-specific polymerase chain reaction.
  
  
  RESULTS
  Six new RHCE alleles were identified, namely, RHCE*cE84A, RHCE*ce202G, RHCE*ce307T, RHCE*Ce377G, RHCE*ce697G,712G,733G,744C, and RHCE*Ce733G.
  
  
  CONCLUSIONS
  While implementation of new assays in commercial genotyping platforms to detect the polymorphisms reported here may not be justified given their apparent rarity, software interpretative algorithms may benefit from the identification of new alleles for a more accurate determination of genotypes and prediction of phenotypes.
authors.affiliation: Canadian Blood Services, Ottawa, Ontario, Canada.
authors.forename: Mindy
authors.initials: M
authors.lastname: Goldman
authors.affiliation: Banco de Sangre y Tejidos De Navarra, Pamplona, Spain.
authors.forename: Arantxa
authors.initials: A
authors.lastname: Cemborain
authors.affiliation: Canadian Blood Services, Ottawa, Ontario, Canada.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Cote
authors.affiliation: Progenika-Grifols, Medford, Massachusetts.
authors.forename: Rachid
authors.initials: R
authors.lastname: El Hamss
authors.affiliation: Australian Red Cross Blood Service, Brisbane, Australia.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Flower
authors.affiliation: Progenika-Grifols, Medford, Massachusetts.
authors.forename: Adirane
authors.initials: A
authors.lastname: Garaizar
authors.affiliation: Centro de Transfusion de Madrid, Madrid, Spain.
authors.forename: Felix
authors.initials: F
authors.lastname: Garcia-Sanchez
authors.affiliation: Australian Red Cross Blood Service, Brisbane, Australia.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Hyland
authors.affiliation: LifeShare Blood Centers, Shreveport, Louisiana.
authors.forename: Monica
authors.initials: M
authors.lastname: Kalvelage
authors.affiliation: Azienda Ospedaliero Universitaria, Udine, Italy.
authors.forename: Donatella
authors.initials: D
authors.lastname: Londero
authors.affiliation: Australian Red Cross Blood Service, Brisbane, Australia.
authors.forename: Genghis H
authors.initials: GH
authors.lastname: Lopez
authors.affiliation: Ospedale Maggiore Policlinico Fondazione IRCCS Ca' Granda, Milano, Italy.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Revelli
authors.affiliation: Banco de Sangre y Tejidos De Navarra, Pamplona, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Rodriguez-Wilhelmi
authors.affiliation: Ospedale Maggiore Policlinico Fondazione IRCCS Ca' Granda, Milano, Italy.
authors.forename: Antonietta
authors.initials: A
authors.lastname: Villa
authors.affiliation: Grifols Shared Services, San Marcos, Texas.
authors.forename: Gorka
authors.initials: G
authors.lastname: Ochoa-Garay
mesh_terms: D000483:Alleles; D044383:Black People / Q000235:genetics*; D001782:Blood Donors; D005819:Genetic Markers; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D010641:Phenotype; D011110:Polymorphism, Genetic; D012204:Rh-Hr Blood-Group System / Q000235:genetics*; D017422:Sequence Analysis, DNA; D044465:White People / Q000235:genetics*
chemical_list: D005819:Genetic Markers; C066468:RHCE protein, human; D012204:Rh-Hr Blood-Group System