title: Transcriptome analysis of hormone-induced gene expression in Brachypodium distachyon.
abstract: Brachypodium distachyon is a new model plant closely related to wheat and other cereals. In this study, we performed a comprehensive analysis of hormone-regulated genes in Brachypodium distachyon using RNA sequencing technology. Brachypodium distachyon seedlings were treated with eight phytohormones (auxin, cytokinine, brassinosteroid, gibberelline, abscisic acid, ethylene, jasmonate and salicylic acid) and two inhibitors, Brz220 (brassinosteroid biosynthesis inhibitor) and prohexadione (gibberelline biosynthesis inhibitor). The expressions of 1807 genes were regulated in a phytohormone-dependent manner. We compared the data with the phytohormone responses that have reported in rice. Transcriptional responses to hormones are conserved between Bracypodium and rice. Transcriptional regulation by brassinosteroid, gibberellin and ethylene was relatively weaker than those by other hormones. This is consistent with the data obtained from comprehensive analysis of hormone responses reported in Arabidopsis. Brachypodium and Arabidopsis also shared some common transcriptional responses to phytohormones. Alternatively, unique transcriptional responses to phytohormones were observed in Brachypodium. For example, the expressions of ACC synthase genes were up-regulated by auxin treatment in rice and Arabidopsis, but no orthologous ACC synthase gene was up-regulated in Brachypodium. Our results provide information useful to understand the diversity and similarity of hormone-regulated transcriptional responses between eudicots and monocots.
authors.affiliation: Kihara Institute for Biological Research, Yokohama City University, Kanagawa, JAPAN.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kakei
authors.affiliation: Kihara Institute for Biological Research, Yokohama City University, Kanagawa, JAPAN.|Cellulose Production Research Team, Biomass Engineering Program Cooperation Division, RIKEN Center for Sustainable Resource Science, Kanagawa, JAPAN.|Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Kanagawa, JAPAN.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Mochida
authors.affiliation: Integrated Genome Informatics Research Unit, RIKEN Center for Sustainable Resource Science, Kanagawa, JAPAN.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakurai
authors.affiliation: Integrated Genome Informatics Research Unit, RIKEN Center for Sustainable Resource Science, Kanagawa, JAPAN.
authors.forename: Takuhiro
authors.initials: T
authors.lastname: Yoshida
authors.affiliation: Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Kanagawa, JAPAN.|Biomass Research Platform Team, Biomass Engineering Research Division, RIKEN Center for Sustainable Resource Science, Kanagawa, JAPAN.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
authors.affiliation: Kihara Institute for Biological Research, Yokohama City University, Kanagawa, JAPAN.|Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Kanagawa, JAPAN.
authors.forename: Yukihisa
authors.initials: Y
authors.lastname: Shimada
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D058431:Brachypodium / Q000187:drug effects* / Q000235:genetics*; D019295:Computational Biology / Q000379:methods; D003583:Cytokinins / Q000494:pharmacology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D005819:Genetic Markers; D059014:High-Throughput Nucleotide Sequencing; D007210:Indoleacetic Acids / Q000494:pharmacology; D012275:Oryza / Q000235:genetics; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology*; D059467:Transcriptome
chemical_list: D003583:Cytokinins; D005819:Genetic Markers; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators