title: Use of Adeno-Associated and Herpes Simplex Viral Vectors for In Vivo Neuronal Expression in Mice.
abstract: Adeno-associated viruses and the herpes simplex virus are the two most widely used vectors for the in vivo expression of exogenous genes. Advances in the development of these vectors have enabled remarkable temporal and spatial control of gene expression. This unit provides methods for storing, delivering, and verifying expression of adeno-associated and herpes simplex viruses in the adult mouse brain. It also describes important considerations for experiments using in vivo expression of these viral vectors, including serotype and promoter selection, as well as timing of expression. Additional protocols are provided that describe methods for preliminary experiments to determine the appropriate conditions for in vivo delivery.
authors.affiliation: McLean Hospital, Harvard Medical School, Belmont, Massachusetts.
authors.forename: Rachel D
authors.initials: RD
authors.lastname: Penrod
authors.affiliation: McLean Hospital, Harvard Medical School, Belmont, Massachusetts.
authors.forename: Audrey M
authors.initials: AM
authors.lastname: Wells
authors.affiliation: McLean Hospital, Harvard Medical School, Belmont, Massachusetts.
authors.forename: William A
authors.initials: WA
authors.lastname: Carlezon
authors.affiliation: McLean Hospital, Harvard Medical School, Belmont, Massachusetts.
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Cowan
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D000229:Dependovirus / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000502:physiology*; D018139:Simplexvirus / Q000235:genetics; D013238:Stereotaxic Techniques
keywords: adeno-associated virus; brain; herpes simplex virus; in vivo; mouse