title: Single Nucleotide Polymorphisms in miR-149 (rs2292832) and miR-101-1 (rs7536540) Are Not Associated with Hepatocellular Carcinoma in Thai Patients with Hepatitis B Virus Infection.
abstract: MicroRNAs directly and indirectly influence many biological processes such as apoptosis, cell maintenance, and immune responses, impacting on tumor genesis and metastasis. They modulate gene expression at the post- transcriptional level and are associated with progression of liver disease. Hepatocellular carcinoma (HCC) is a cancer which mostly occurs in males. There are many factors affect HCC development, for example, hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV), co-infection, environmental factors including alcohol, aflatoxin consumption and host-related factors such as age, gender immune response, microRNA and single nucleotide polymorphisms (SNPs). Chronic infection with the hepatitis B virus is the major factor leading to HCC progression since it causes the liver injury. At present, there are many reports regarding the association of SNPs on miRNAs and the HCC progression. In this research, we investigated the role of miR- 149 (rs2292832) and miR-101-1 (rs7536540) with HCC progression in Thai population. The study included 289 Thai subjects including 104 HCC patients, 90 patients with chronic hepatitis B virus infection (CHB) and 95 healthy control subjects. The allele and genotype of rs2292832 and rs7536540 polymorphisms were determined by TaqMan real-time PCR assay. Our results revealed no significant association between miR-149 (rs2292832) and miR-101-1 (rs7536540) and the risk of HCC in our Thai population. However, this research is the first study of miR-149 (rs2292832) and miR-101-1 (rs7536540) in HCC in Thai populations and the results need to be confirmed with a larger population.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Thailand E-mail : sp.medbiochemcu@gmail.com.
authors.forename: Pornpitra
authors.initials: P
authors.lastname: Pratedrat
authors.forename: Watanyoo
authors.initials: W
authors.lastname: Sopipong
authors.forename: Jarika
authors.initials: J
authors.lastname: Makkoch
authors.forename: Kesmanee
authors.initials: K
authors.lastname: Praianantathavorn
authors.forename: Natthaya
authors.initials: N
authors.lastname: Chuaypen
authors.forename: Pisit
authors.initials: P
authors.lastname: Tangkijvanich
authors.forename: Sunchai
authors.initials: S
authors.lastname: Payungporn
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology / Q000821:virology; D016022:Case-Control Studies; D018450:Disease Progression; D005260:Female; D005787:Gene Frequency; D006515:Hepatitis B virus; D019694:Hepatitis B, Chronic / Q000150:complications; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D013785:Thailand
chemical_list: C534072:MIRN101 microRNA, human; C551557:MIRN149 microRNA, human; D035683:MicroRNAs