title: Investigation of the Association Between 584C/T Polymorphism of EL Gene and Risk of Premature Coronary Artery Disease in Fars Province.
abstract: BACKGROUND
  Endothelial lipase (EL) is a protein from the triglyceride lipase family which plays an important role in high-density lipoprotein (HDL) metabolism. One of the most frequently studied variants is 584C/T which causes the amino acid threonine at codon 111 to convert to isoleucine. Many studies have shown the association of this variant with HDL-C level and CAD disease.
  
  
  METHODS
  The population of this study consists of 140 patients (all males) with angiographically confirmed coronary artery disease (CAD) and 80 controls. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) was carried out for genotyping of LIPG 584C/T. Data were analyzed using SPSS.
  
  
  RESULTS
  The results of the study indicated that the frequency of T allele was significantly lower among CAD patients than among controls (0.27 vs 0.36, P = .004). However, no significant correlation was found between the 584C/T variant and serum HDL-C level. Multivariate regression analysis confirmed that the T allele is significantly associated with CAD disregarding the age, hypertension, hypercholesterolemia, diabetes and HDL-C (OR = 0.494, 95% CI = 0.253- 0.968, P =.040).
  
  
  CONCLUSIONS
  It was concluded that the T allele was associated with protection from CAD in Fars province independent of HDL-C level.
authors.affiliation: Human Genetic Research Group, Iranian Academic Center for Education, Culture & Research (ACECR), Fars Branch, Shiraz, Iran.
authors.forename: Samane
authors.initials: S
authors.lastname: Toosi
authors.affiliation: Human Genetic Research Group, Iranian Academic Center for Education, Culture & Research (ACECR), Fars Branch, Shiraz, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Senemar
authors.affiliation: Human Genetic Research Group, Iranian Academic Center for Education, Culture & Research (ACECR), Fars Branch, Shiraz, Iran.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Ahmadi
authors.affiliation: Department of Cardiology, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Salma
authors.initials: S
authors.lastname: Radmanesh
keywords: 584C/T; Coronary Artery; Endothelial Lipase; Polymorphism