title: Effect of a mouthrinse containing rice peptide CL(14-25) on early dental plaque regrowth: a randomized crossover pilot study.
abstract: BACKGROUND
  We aimed to evaluate clinically the effect of mouthrinse containing a rice peptide on early dental plaque regrowth.
  
  
  METHODS
  The study was designed as a double-masked, two-group crossover randomized pilot trial, involving 10 periodontally healthy volunteers. After receiving a professional tooth cleaning at baseline, over the next 3 days each participant refrained from all oral hygiene measures and had two daily rinses with 20 ml of the test mouthrinse containing 0.4 % rice peptide CL(14-25) or placebo rinse. At the end of each experimental period, plaque score was assessed using the modified Volpe's method, and the participants filled out a questionnaire. Each participant underwent a 7-day washout period followed by a second allocation. The plaque score was the primary outcome of the study and participant perception was the secondary outcome.
  
  
  RESULTS
  No adverse effects were observed in the participants during the study. Clinically, the mean plaque score of the examined teeth was significantly lower in the test group (2.44 ± 0.74, CI: 1.91-2.96) than the placebo group (2.65 ± 0.63, CI: 2.20-3.10) (P < 0.05). When analyzed according to the type of teeth, a significantly lower score of the premolars/molars was observed in the test group (2.39 ± 0.68, CI: 2.08-2.71) than that in the placebo group (2.66 ± 0.58, CI: 2.39-2.93) (P < 0.05).
  
  
  CONCLUSIONS
  The mouthrinse containing 0.4 % rice peptide CL(14-25) was effective in reducing the early regrowth of dental plaque. However, clinical relevance of this efficacy needs to be validated in a future large-scale study.
  
  
  BACKGROUND
  UMIN Clinical Trials Registry (UMIN-CTR) R000014000. Date of formal registration: November 1, 2013.
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan. saoritakayama@tdc.ac.jp.
authors.forename: Saori
authors.initials: S
authors.lastname: Takayama
authors.affiliation: Laboratory of Chemistry, Tokyo Dental College, Tokyo, Japan. tekato@tdc.ac.jp.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kato
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan. imamurakentarou@tdc.ac.jp.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Imamura
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan. kitadaichi@tdc.ac.jp.
authors.forename: Daichi
authors.initials: D
authors.lastname: Kita
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan. ootakouki@tdc.ac.jp.|Oral Health Science Center, Tokyo Dental College, Tokyo, Japan. ootakouki@tdc.ac.jp.
authors.forename: Koki
authors.initials: K
authors.lastname: Ota
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan. suzukieiichi@tdc.ac.jp.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Suzuki
authors.affiliation: Department of Endodontics and Clinical Cariology, Tokyo Dental College, Tokyo, Japan. sugito@tdc.ac.jp.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sugito
authors.affiliation: Graduate School of Technology, Niigata Institute of Technology, Niigata, Japan. esaitoh@acb.niit.ac.jp.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Saitoh
authors.affiliation: Department of Materials Science and Technology, Graduate School of Science and Technology, Niigata University, Niigata, Japan. mtanig@eng.niigata-u.ac.jp.|Center for Transdisciplinary Research, Niigata University, Niigata, Japan. mtanig@eng.niigata-u.ac.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Taniguchi
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan. atsaito@tdc.ac.jp.|Oral Health Science Center, Tokyo Dental College, Tokyo, Japan. atsaito@tdc.ac.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Saito
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D003773:Dental Plaque / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009067:Mouthwashes / Q000627:therapeutic use*; D012275:Oryza / Q000737:chemistry*; D010349:Patient Compliance; D010455:Peptides / Q000737:chemistry / Q000627:therapeutic use*; D010465:Perception; D010865:Pilot Projects
chemical_list: D009067:Mouthwashes; D010455:Peptides