title: Imaging Newborn Granule Cells in Fixed Sections.
abstract: This protocol describes the harvesting of brain tissue from mice that have had the retroviral vector CAG-GFP injected into the dentate gyrus. Brain tissue from these mice is dissected, the tissue is fixed, and the sections are prepared. The fixed sections are imaged using fluorescent confocal microscopy, and newborn granule cells containing GFP are visualized and are characterized.
authors.forename: Ayumu
authors.initials: A
authors.lastname: Tashiro
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhao
authors.forename: Hoonkyo
authors.initials: H
authors.lastname: Suh
authors.forename: Fred H
authors.initials: FH
authors.lastname: Gage
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology*; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D008867:Microtomy; D009474:Neurons / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation
chemical_list: D049452:Green Fluorescent Proteins