title: Fast Screening of Whole Blood Samples and Pharmaceutical Compounds for Enantiorecognition of Free L-T3 , L-T4 , and D-T4.
abstract: A fast screening method of whole blood was proposed for enantiorecognition of free L-T3 , L-T4 , and D-T4 . Stochastic microsensors based on four inulins (IN, IQ, TEX, and HD) immobilized on diamond paste (DP) were used for recognition of free L-T3 , L-T4 , and D-T4 . For the enantiorecognition of free L-T4 and D-T4 in whole blood and pharmaceutical samples, the best microsensor was the one based on TEX/DP (wide linear concentration ranges, and low limits of quantification). The best limit of detection for the assay of free L-T3 (400 fmol/L) was recorded using the microsensors based on HD/DP, while for the assay of free L-T4, and D-T4 the best limit of determination (1 pmol/L) was recorded using the TX/DP-based microsensor. For the enantiorecognition of free L-T3 in whole blood and pharmaceutical samples the best microsensor was the one based on HD/DP (the wider linear concentration range, and the lower limit of quantification - of pmol/L magnitude order). For the enantiorecognition of free L-T3 in whole blood and pharmaceutical samples the best microsensor was the one based on HD/DP (the wider linear concentration range, and the lower limit of quantification - of pmol/L magnitude order). Free L-T3 , L-T4 , and D-T4 were recovered with high reliabilities in whole blood samples (recoveries higher than 99.00%, with RSD values lower than 1.00%) and pharmaceutical samples (recoveries higher than 95.00% with RSD values lower than 1.00%).
authors.affiliation: Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, Bucharest, Romania.
authors.forename: Grigorina
authors.initials: G
authors.lastname: Mitrofan
authors.affiliation: Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, Bucharest, Romania.|Laboratory of Electrochemistry and PATLAB Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, Bucharest, Romania.
authors.forename: Raluca-Ioana
authors.initials: RI
authors.lastname: Stefan-van Staden
authors.affiliation: Laboratory of Electrochemistry and PATLAB Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, Bucharest, Romania.
authors.forename: Ionela Raluca
authors.initials: IR
authors.lastname: Comnea-Stancu
authors.affiliation: Laboratory of Electrochemistry and PATLAB Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, Bucharest, Romania.
authors.forename: Jacobus Frederick
authors.initials: JF
authors.lastname: van Staden
authors.affiliation: Pharmaco-Bromatology & Molecular Nutrition, Faculty of Pharmacy, Nicolaus Copernicus University, Bydgoszcz, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bazylak
authors.affiliation: University of Cyprus, Nicosia, Cyprus.
authors.forename: Constantina P
authors.initials: CP
authors.lastname: Kapnissi-Christodoulou
authors.affiliation: Pharmaceutical and Medicinal Chemistry, Pharmaceutical and Drug Industries Research Division, National Research Centre, Giza, Egypt.
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
mesh_terms: D006801:Humans; D057230:Limit of Detection; D013237:Stereoisomerism; D013974:Thyroxine / Q000097:blood*; D014284:Triiodothyronine / Q000097:blood*
chemical_list: D014284:Triiodothyronine; D013974:Thyroxine
keywords: enantiorecognition; fast screening; inulins; stochastic microsensors; thyroid hormones