title: Foxp3-expressing sensitized Teff cells prolong survival of corneal allograft in corneal allograft transplantation mouse model.
abstract: OBJECTIVE
  The study aimed to investigate whether Foxp3-expressing sensitized Teff cells could inhibit allograft rejection in corneal allograft transplantation mouse model.
  
  
  METHODS
  Foxp3-expressing sensitized Teff cells were constructed by transfection of retroviral expression plasmid expressing Foxp3 into the sensi-Teff cells from a Balb/c mouse immunized by C57BL/6(H2b) mouse splenocytes. Balb/c mice were randomly divided into 5 groups: Four groups received tail vein injection of Foxp3-expressing sensitized Teff cells, or Foxp3-expressing Teff cells, or Treg cells or no intervention 1 day prior to corneal allograft transplantation. C57BL/6(H2b) was the donor mouse. The last group received corneal autograft transplantation. Corneal allograft survival time and percentage of CD4(+) T cells were detected. ELISPOT and Footpad swelling test were used to measure IL-2 and IFN-γ, and delayed-type hypersensitivity (DTH) response, respectively.
  
  
  RESULTS
  Mice that had received an injection of Foxp3-expressing sensitized T cells prior to an allograft corneal transplantation, showed significantly longer survival time of corneal allograft, decreased percentage of CD4(+) T cells, IL-2 and IFN-γ, and alleviated footpad swelling than the mice that had received either Foxp3-Teff or Treg cells.
  
  
  CONCLUSIONS
  Foxp3-sensi-Teff cell treatment that prolongs corneal allograft survival in the mouse model, might partly through suppressing CD4(+) T cells, IL-2 and IFN-γ.
authors.affiliation: Department of Ophthalmology, The 2nd Hospital of Shandong University, Jinan 250031, China; Department of Ophthalmology, General Hospital of Liaohe Oil Field, Panjin 124000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: The General Hospital of PLA, Beijing 100853, China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, The 2nd Hospital of Shandong University, Jinan 250031, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, The 2nd Hospital of Shandong University, Jinan 250031, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: The General Hospital of PLA, Beijing 100853, China.
authors.forename: Dajiang
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, The 2nd Hospital of Shandong University, Jinan 250031, China.
authors.forename: Guoling
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, General Hospital of Liaohe Oil Field, Panjin 124000, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, The 2nd Hospital of Shandong University, Jinan 250031, China. Electronic address: han1_zhang@163.com.
authors.forename: Han
authors.initials: H
authors.lastname: Zhang
mesh_terms: D064591:Allografts / Q000276:immunology; D000818:Animals; D002478:Cells, Cultured; D016039:Corneal Transplantation; D004195:Disease Models, Animal; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D006084:Graft Rejection / Q000276:immunology*; D006085:Graft Survival / Q000276:immunology; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007165:Immunosuppression Therapy; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D006377:T-Lymphocytes, Helper-Inducer / Q000502:physiology* / Q000637:transplantation; D050378:T-Lymphocytes, Regulatory / Q000502:physiology* / Q000637:transplantation
chemical_list: D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D007376:Interleukin-2; D007371:Interferon-gamma
keywords: Allograft rejection; Corneal allograft; Delayed-type hypersensitivity; Foxp3; Treg cell