title: Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation.
abstract: Marine diatoms have recently gained much attention as they are expected to be a promising resource for sustainable production of bioactive compounds such as carotenoids and biofuels as a future clean energy solution. To develop photosynthetic cell factories, it is important to improve diatoms for value-added products. In this study, we utilized UVC radiation to induce mutations in the marine diatom Phaeodactylum tricornutum and screened strains with enhanced accumulation of neutral lipids and carotenoids. Adaptive laboratory evolution (ALE) was also used in parallel to develop altered phenotypic and biological functions in P. tricornutum and it was reported for the first time that ALE was successfully applied on diatoms for the enhancement of growth performance and productivity of value-added carotenoids to date. Liquid chromatography-mass spectrometry (LC-MS) was utilized to study the composition of major pigments in the wild type P. tricornutum, UV mutants and ALE strains. UVC radiated strains exhibited higher accumulation of fucoxanthin as well as neutral lipids compared to their wild type counterpart. In addition to UV mutagenesis, P. tricornutum strains developed by ALE also yielded enhanced biomass production and fucoxanthin accumulation under combined red and blue light. In short, both UV mutagenesis and ALE appeared as an effective approach to developing desired phenotypes in the marine diatoms via electromagnetic radiation-induced oxidative stress.
authors.affiliation: Center for Systems Biology and Faculty of Industrial Engineering, Mechanical Engineering and Computer Science, School of Engineering and Natural Sciences, University of Iceland, Reykjavík 101, Iceland. zhy1@hi.is.|Biomedical Center and Department of Life and Environmental Sciences, University of Iceland, Reykjavík 101, Iceland. zhy1@hi.is.
authors.forename: Zhiqian
authors.initials: Z
authors.lastname: Yi
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Iceland, Hagi, Hofsvallagata 53, Reykjavik IS-107, Iceland. xum1@hi.is.
authors.forename: Maonian
authors.initials: M
authors.lastname: Xu
authors.affiliation: Center for Systems Biology and Faculty of Industrial Engineering, Mechanical Engineering and Computer Science, School of Engineering and Natural Sciences, University of Iceland, Reykjavík 101, Iceland. mam5@hi.is.
authors.forename: Manuela
authors.initials: M
authors.lastname: Magnusdottir
authors.affiliation: Center for Systems Biology and Faculty of Industrial Engineering, Mechanical Engineering and Computer Science, School of Engineering and Natural Sciences, University of Iceland, Reykjavík 101, Iceland. yuetuan.zhang@helsinki.fi.|Department of Food and Environmental Sciences, Division of Food Chemistry, University of Helsinki, Helsinki FI-00014, Finland. yuetuan.zhang@helsinki.fi.
authors.forename: Yuetuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center for Systems Biology and Faculty of Industrial Engineering, Mechanical Engineering and Computer Science, School of Engineering and Natural Sciences, University of Iceland, Reykjavík 101, Iceland. sb@hi.is.
authors.forename: Sigurdur
authors.initials: S
authors.lastname: Brynjolfsson
authors.affiliation: Center for Systems Biology and Faculty of Industrial Engineering, Mechanical Engineering and Computer Science, School of Engineering and Natural Sciences, University of Iceland, Reykjavík 101, Iceland. weiqi@hi.is.|Division of Science and Math, New York University Abu Dhabi, and Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi Institute, Abu Dhabi 129188, UAE. weiqi@hi.is.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Fu
mesh_terms: D018533:Biomass; D002338:Carotenoids / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D017377:Diatoms / Q000235:genetics / Q000378:metabolism*; D060733:Electromagnetic Radiation; D050356:Lipid Metabolism / Q000528:radiation effects; D013058:Mass Spectrometry / Q000379:methods; D016296:Mutagenesis / Q000528:radiation effects*; D009154:Mutation; D018384:Oxidative Stress / Q000528:radiation effects*; D014466:Ultraviolet Rays; D024341:Xanthophylls / Q000378:metabolism
chemical_list: D024341:Xanthophylls; C025164:fucoxanthin; D002338:Carotenoids
keywords: Phaeodactylum tricornutum; UV mutagenesis; adaptive laboratory evolution (ALE); fucoxanthin; neutral lipids