title: Cloning, expression and biochemical characterization of recombinant superoxide dismutase from Antarctic psychrophilic bacterium Pseudoalteromonas sp. ANT506.
abstract: In this study, a superoxide dismutase gene (PsSOD) from Pseudoalteromonas sp. ANT506 was cloned and over expressed in Escherichia coli. The PsSOD has an open reading frame of 582 bp with a putative product of 193 amino acid residue and an estimated molecular size of 21.4 kDa. His-tagged PsSOD was subsequently purified 12.6-fold by Ni-affinity chromatography and the yield of 22.9%. The characterization of the purified rPsSOD exhibited maximum activity at 30 °C and pH 8.0. The enzyme exhibited 13.9% activity at 0 °C and had high-thermo lability at higher than 50 °C. rPsSOD exhibited well capability to 2.5 M NaCl (62.4%). These results indicated that rPsSOD exhibited special catalytic properties.
authors.affiliation: School of Marine and Technology, Harbin Institute of Technology, Weihai, P.R. China.
authors.forename: Quan-Fu
authors.initials: QF
authors.lastname: Wang
authors.affiliation: School of Marine and Technology, Harbin Institute of Technology, Weihai, P.R. China.
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Wang
authors.affiliation: School of Marine and Technology, Harbin Institute of Technology, Weihai, P.R. China.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Hou
authors.affiliation: School of Marine and Technology, Harbin Institute of Technology, Weihai, P.R. China.
authors.forename: Yong-Lei
authors.initials: YL
authors.lastname: Shi
authors.affiliation: School of Marine and Technology, Harbin Institute of Technology, Weihai, P.R. China.
authors.forename: Han
authors.initials: H
authors.lastname: Han
authors.affiliation: School of Marine and Technology, Harbin Institute of Technology, Weihai, P.R. China.
authors.forename: Miao
authors.initials: M
authors.lastname: Miao
authors.affiliation: School of Marine and Technology, Harbin Institute of Technology, Weihai, P.R. China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Wu
authors.affiliation: Shandong Provincial Engineering Technology Research Center of Marine Health Food, Rongcheng, P.R. China.
authors.forename: Yuan-Ping
authors.initials: YP
authors.lastname: Liu
authors.affiliation: Shandong Provincial Research Institute of Marine Food Nutrition, Rongcheng, P.R. China.
authors.forename: Xiao-Na
authors.initials: XN
authors.lastname: Yue
authors.affiliation: National and Local United Engineering Laboratory of Marine Functional Food Development (Shandong), Rongcheng, P.R. China.
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000864:Antarctic Regions; D001426:Bacterial Proteins / Q000235:genetics*; D002384:Catalysis; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D016366:Open Reading Frames / Q000235:genetics; D031161:Pseudoalteromonas / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase
keywords: Characterization; Cold-adapted; Expression; Sea-ice; Superoxide dismutase