title: Eukaryotic Mismatch Repair in Relation to DNA Replication.
abstract: Three processes act in series to accurately replicate the eukaryotic nuclear genome. The major replicative DNA polymerases strongly prevent mismatch formation, occasional mismatches that do form are proofread during replication, and rare mismatches that escape proofreading are corrected by mismatch repair (MMR). This review focuses on MMR in light of increasing knowledge about nuclear DNA replication enzymology and the rate and specificity with which mismatches are generated during leading- and lagging-strand replication. We consider differences in MMR efficiency in relation to mismatch recognition, signaling to direct MMR to the nascent strand, mismatch removal, and the timing of MMR. These studies are refining our understanding of relationships between generating and repairing replication errors to achieve accurate replication of both DNA strands of the nuclear genome.
authors.affiliation: Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, North Carolina 27709; email: kunkel@niehs.nih.gov.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Kunkel
authors.affiliation: Department of Chemistry and Curriculum in Applied Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina 27599-3290; email: derie@email.unc.edu.
authors.forename: Dorothy A
authors.initials: DA
authors.lastname: Erie
mesh_terms: D004247:DNA / Q000235:genetics; D004249:DNA Damage; D053843:DNA Mismatch Repair / Q000502:physiology*; D004261:DNA Replication / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics; D042822:Genomic Instability; D006801:Humans; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D004247:DNA
keywords: DNA mismatch repair; genome instability; mutation rate; mutator; replication fidelity