title: Mapping Synaptic Input Fields of Neurons with Super-Resolution Imaging.
abstract: As a basic functional unit in neural circuits, each neuron integrates input signals from hundreds to thousands of synapses. Knowledge of the synaptic input fields of individual neurons, including the identity, strength, and location of each synapse, is essential for understanding how neurons compute. Here, we developed a volumetric super-resolution reconstruction platform for large-volume imaging and automated segmentation of neurons and synapses with molecular identity information. We used this platform to map inhibitory synaptic input fields of On-Off direction-selective ganglion cells (On-Off DSGCs), which are important for computing visual motion direction in the mouse retina. The reconstructions of On-Off DSGCs showed a GABAergic, receptor subtype-specific input field for generating direction selective responses without significant glycinergic inputs for mediating monosynaptic crossover inhibition. These results demonstrate unique capabilities of this super-resolution platform for interrogating neural circuitry.
authors.affiliation: Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Yaron M
authors.initials: YM
authors.lastname: Sigal
authors.affiliation: Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Colenso M
authors.initials: CM
authors.lastname: Speer
authors.affiliation: Center for Brain Science, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Hazen P
authors.initials: HP
authors.lastname: Babcock
authors.affiliation: Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA; Center for Brain Science, Harvard University, Cambridge, MA 02138, USA; Department of Physics, Harvard University, Cambridge, MA 02138, USA. Electronic address: zhuang@chemistry.harvard.edu.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhuang
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D002352:Carrier Proteins; D007150:Immunohistochemistry; D008565:Membrane Proteins; D051379:Mice; D009415:Nerve Net; D009434:Neural Pathways; D009474:Neurons / Q000166:cytology*; D061848:Optical Imaging / Q000379:methods*; D018079:Receptors, GABA / Q000378:metabolism; D018009:Receptors, Glycine / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000378:metabolism; D055351:Retinal Neurons / Q000378:metabolism; D013569:Synapses / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D018079:Receptors, GABA; D018009:Receptors, Glycine; C073441:gephyrin