title: Nanostructured Capsules for Cartilage Tissue Engineering.
abstract: Polymeric multilayered capsules (PMCs) have found great applicability in bioencapsulation, an evolving branch of tissue engineering and regenerative medicine. Here, we describe the production of hierarchical PMCs composed by an external multilayered membrane by layer-by-layer assembly of poly(L-lysine), alginate, and chitosan. The core of the PMCs is liquified and encapsulates human adipose stem cells and surface-functionalized collagen II-TGF-β3 poly(L-lactic acid) microparticles for cartilage tissue engineering.
authors.affiliation: 3B' s Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal.|ICVS/3B's-PT Government Associate Laboratory, Guimarães, Braga, Portugal.
authors.forename: Clara R
authors.initials: CR
authors.lastname: Correia
authors.affiliation: 3B' s Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal.|ICVS/3B's-PT Government Associate Laboratory, Guimarães, Braga, Portugal.
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
authors.affiliation: 3B' s Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal. jmano@dep.uminho.pt.|ICVS/3B's-PT Government Associate Laboratory, Guimarães, Braga, Portugal. jmano@dep.uminho.pt.
authors.forename: João F
authors.initials: JF
authors.lastname: Mano
mesh_terms: D000464:Alginates / Q000737:chemistry; D002356:Cartilage / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000637:transplantation; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D020219:Chondrogenesis / Q000187:drug effects; D024043:Collagen Type II / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D053769:Nanocapsules; D050997:Nanomedicine / Q000379:methods*; D011107:Polylysine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012038:Regeneration; D044968:Regenerative Medicine / Q000379:methods*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D050151:Subcutaneous Fat / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D053782:Transforming Growth Factor beta3 / Q000008:administration & dosage / Q000378:metabolism
chemical_list: D000464:Alginates; D024043:Collagen Type II; D006603:Hexuronic Acids; D053769:Nanocapsules; D011108:Polymers; D053782:Transforming Growth Factor beta3; D011107:Polylysine; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Bioencapsulation; Capsules; Cartilage regeneration; Collagen II; Layer-by-layer; Microparticles; Stem cells; TGF-β3; Tissue engineering