title: A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans Dauer Larvae.
abstract: The C. elegans dauer is an attractive model with which to investigate fundamental biological questions, such as how environmental cues are sensed and are translated into developmental decisions through a series of signaling cascades that ultimately result in a transformed animal. Here we describe a simple method of using egg white plates to obtain highly synchronized purified dauers that can be used in downstream applications requiring large quantities of dauers or postdauer animals.
authors.affiliation: Department of Biology, Syracuse University, 107 College Place, Room 110, Syracuse, NY, 13244, USA.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Ow
authors.affiliation: Department of Biology, Syracuse University, 107 College Place, Room 110, Syracuse, NY, 13244, USA. shall@syr.edu.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Hall
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D007814:Larva
keywords: Caenorhabditis elegans; Dauer; Diapause; Egg white plate; Pheromone; Postdauer; Stress