title: Lipid peroxidation, proteins modifications, anti-oxidant enzymes activities and selenium deficiency in the plasma of hashitoxicosis patients.
abstract: OBJECTIVE
  The aim of this study was to explore the oxidative stress profile in hashitoxicosis (HTX) and to compare it with that of healthy subjects.
  
  
  METHODS
  Spectrophotometric methods were used to evaluate the oxidative stress markers. The selenium level was investigated by atomic absorption.
  
  
  RESULTS
  High levels of thiobarbituric acid reactive species (TBARS) and conjugated dienes were found in HTX patients (p = 0.034 and p = 0.043, respectively) compared with healthy controls. For antioxidant enzymes, superoxide dismutase (SOD) and catalase activities increased, whereas that of glutathione peroxidase (GPx) decreased (p = 0.000, p = 0.014, p = 0.000, respectively) compared with controls. A reduction in the level of selenium (p = 0.029) and thiol groups (p = 0.008) were shown in patients; however, levels of carbonyl group and malondialdehyde (MDA) protein adducts decreased (p = 0.000) compared with controls. Positive correlation was shown between levels of free thyroxine (FT4) and TBARS (r = 0.711, p = 0.048) and between FT4 level and SOD activity (r = 0.713, p = 0.047). Conversely, GPx activity presented a negative correlation with FT4 and free triiodothyronine (FT3) levels (r = -0.934, p = 0.001; r = -0.993, p = 0.000, respectively). In addition, GPx activity showed positive correlation with selenium level (r = 0.981, p = 0.019) and the FT3 level correlated negatively with the level of thiol groups (r = -0.892, p = 0.017).
  
  
  CONCLUSIONS
  This study shows the presence of an oxidative stress and selenium deficiency in HTX patients and suggests that the hyperthyroid state is strongly implicated in the establishment of this disturbed oxidative profile.
authors.affiliation: National School of Engineers of Sfax, Sokra Street, km3, Sfax, 3000 Tunisia.
authors.forename: Malek
authors.initials: M
authors.lastname: Mseddi
authors.affiliation: National School of Engineers of Sfax, Sfax, Tunisia.
authors.forename: Riadh
authors.initials: R
authors.lastname: Ben Mansour
authors.affiliation: Department of Endocrinology and Diabetes Hedi Chaker, Sfax, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Mnif
authors.affiliation: National School of Engineers of Sfax, Sfax, Tunisia.
authors.forename: Bochra
authors.initials: B
authors.lastname: Gargouri
authors.affiliation: Department of Endocrinology and Diabetes Hedi Chaker, Sfax, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abid
authors.affiliation: Department of Nuclear Medicine, Habib Bourguiba, Sfax, Tunisia.
authors.forename: Fadhel
authors.initials: F
authors.lastname: Guermazi
authors.affiliation: National School of Engineers of Sfax, Sfax, Tunisia.
authors.forename: Hamadi
authors.initials: H
authors.lastname: Attia
authors.affiliation: National School of Engineers of Sfax, Sfax, Tunisia.
authors.forename: Saloua
authors.initials: S
authors.lastname: Lassoued
keywords: antioxidant enzymes; carbonyl; hashitoxicosis; malondialdehyde; selenium