title: De Novo Transcriptome Assembly and Comparative Analysis Elucidate Complicated Mechanism Regulating Astragalus chrysochlorus Response to Selenium Stimuli.
abstract: Astragalus species are medicinal plants that are used in the world for years. Some Astragalus species are known for selenium accumulation and tolerance and one of them is Astragalus chrysochlorus, a secondary selenium accumulator. In this study, we employed Illumina deep sequencing technology for the first time to de novo assemble A. chrysochlorus transcriptome and identify the differentially expressed genes after selenate treatment. Totally, 59,656 unigenes were annotated with different databases and 53,960 unigenes were detected in NR database. Transcriptome in A. chrysochlorus is closer to Glycine max than other plant species with 43,1 percentage of similarity. Annotated unigenes were also used for gene ontology enrichment and pathway enrichment analysis. The most significant genes and pathways were ABC transporters, plant pathogen interaction, biosynthesis of secondary metabolites and carbohydrate metabolism. Our results will help to enlighten the selenium accumulation and tolerance mechanisms, respectively in plants.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, 34134 Vezneciler Istanbul, Turkey; Department of Biology, East Carolina University, Greenville, NC 27858, United States of America.
authors.forename: Özgür
authors.initials: Ö
authors.lastname: Çakır
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, 34134 Vezneciler Istanbul, Turkey.
authors.forename: Neslihan
authors.initials: N
authors.lastname: Turgut-Kara
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, 34134 Vezneciler Istanbul, Turkey; Research and Application Center for Biotechnology and Genetic Engineering, Istanbul University, 34134, Istanbul, Turkey.
authors.forename: Şule
authors.initials: Ş
authors.lastname: Arı
authors.affiliation: Department of Biology, East Carolina University, Greenville, NC 27858, United States of America.
authors.forename: Baohong
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D027883:Astragalus Plant / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D018749:RNA, Plant / Q000235:genetics*; D012643:Selenium / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D018749:RNA, Plant; D012643:Selenium