title: Prevalence of CCR5-Δ32 and CCR2-V64I polymorphisms in a mixed population from northeastern Brazil.
abstract: Chemokines are low-molecular weight proteins that play a key role in inflammatory processes. Genomic variations in chemokine receptors are associated with the susceptibility to various diseases. Polymorphisms in chemokine receptor type 5 (CCR5)-Δ32 and CCR2-V64I are related to human immunodeficiency virus infection resistance, which has led to genetic association studies for several other diseases. Given the heterogeneous distribution of these polymorphisms in different global populations and within Brazilian populations, we analyzed the prevalence of CCR5-Δ32 and CCR2-V64I polymorphisms in a mixed population from northeastern Brazil. The study included 223 individuals from the general population of the city of Parnaíba, Piauí, who had a mean age of 73 years. Of these individuals, 37.2% were men and 62.8% were women. Polymorphisms were analyzed using DNA extracted from peripheral blood leukocytes by using polymerase chain reaction alone (CCR5-Δ32) or accompanied by restriction endonuclease digestion (CCR2-V64I). In both cases, the genotypes were determined using 8% polyacrylamide gel electrophoresis and silver nitrate staining. The population conformed to Hardy-Weinberg equilibrium for both the loci studied. No individuals were homozygous for allele-Δ32, which was present in 1.8% of the population, whereas allele-64I was present in 13.9% of the participants studied; 74.9% were homozygous for the wild-type allele, while 22.4 and 2.7% were heterozygous and homozygous for the mutant allele, respectively. Additional studies are needed to investigate the relationship between these polymorphisms and disease etiopathogenesis in reference populations.
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal do Piauí, Parnaíba, PI, Brasil.
authors.forename: H
authors.initials: H
authors.lastname: Ferreira-Fernandes
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal do Piauí, Parnaíba, PI, Brasil.
authors.forename: A C C
authors.initials: AC
authors.lastname: Santos
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal do Piauí, Parnaíba, PI, Brasil.
authors.forename: F J N
authors.initials: FJ
authors.lastname: Motta
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal do Piauí, Parnaíba, PI, Brasil.
authors.forename: R
authors.initials: R
authors.lastname: Canalle
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal do Piauí, Parnaíba, PI, Brasil.
authors.forename: F K N
authors.initials: FK
authors.lastname: Yoshioka
authors.affiliation: Laboratório de Citogenética Humana, Departamento de Biologia, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: R R
authors.initials: RR
authors.lastname: Burbano
authors.affiliation: Laboratório de Oncogenética Molecular, Departamento de Cirugía Experimental, Hospital Universitario La Paz, Madrid, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Rey
authors.affiliation: Hospital Universitário, Universidade Federal do Piauí, Teresina, PI, Brasil.
authors.forename: B B
authors.initials: BB
authors.lastname: da Silva
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal do Piauí, Parnaíba, PI, Brasil.
authors.forename: G R
authors.initials: GR
authors.lastname: Pinto
mesh_terms: D000368:Aged; D000483:Alleles; D044467:American Indian or Alaska Native; D044383:Black People; D001938:Brazil; D005260:Female; D015870:Gene Expression / Q000276:immunology; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D054390:Receptors, CCR2 / Q000235:genetics* / Q000276:immunology; D019713:Receptors, CCR5 / Q000235:genetics* / Q000276:immunology; D044465:White People
chemical_list: C515334:CCR2 protein, human; C584933:CCR5 protein, human; D054390:Receptors, CCR2; D019713:Receptors, CCR5