title: Expression of surfactant proteins SP-A and SP-D in murine decidua and immunomodulatory effects on decidual macrophages.
abstract: Surfactant proteins SP-A and SP-D are pattern recognition innate immune molecules that belong to the C-type lectin family. In lungs, they play an important role in the clearance of pathogens and control of inflammation. SP-A and SP-D are also expressed in the female reproductive tract where they play an important role in pregnancy and parturition. However, the role of SP-A and SP-D expressed at the feto-maternal interface (decidua) remains unclear. Here, we have examined the expression of SP-A and SP-D in the murine decidua at 17.5 (pre-parturition) and 19.5dpc (near parturition) and their effect on lipopolysaccharide (LPS)-treated decidual macrophages. SP-A and SP-D were localized to stromal cells in the murine decidua at 17.5 and 19.5dpc in addition to cells lining the maternal spiral artery. Purified pre-parturition decidual cells were challenged with LPS with and without SP-A or SP-D, and expression of F4/80 and TNF-α were measured by flow cytometry. On their own, SP-A or SP-D did not affect the percentage of F4/80 positive cells while they suppressed the percentage of TNF-α positive cells. However, simultaneous addition of SP-A or SP-D, together with LPS, reduced TNF-α secreting F4/80 positive cells. It is likely that exogenous administration of SP-A and SP-D in decidua can potentially control infection and inflammation mediators during spontaneous term labor and infection-induced preterm labor. Thus, the presence of SP-A and SP-D in the murine decidua is likely to play a protective role against intrauterine infection during pregnancy.
authors.affiliation: Department of Innate Immunity, National Institute for Research in Reproductive Health, Mumbai 400 012, India; Centre for Biotechnology and Bioinformatics, School of Life Sciences, Jawaharlal Nehru Institute for Advanced Studies, Secunderabad, Telangana, India; Centre for Infection, Immunity and Disease Mechanisms, College of Health and Life Science, Brunel University London, Uxbridge, UB8 3PH, United Kingdom.
authors.forename: Shanmuga Priyaa
authors.initials: SP
authors.lastname: Madhukaran
authors.affiliation: Department of Innate Immunity, National Institute for Research in Reproductive Health, Mumbai 400 012, India.
authors.forename: Aghila Rani
authors.initials: AR
authors.lastname: Koippallil Gopalakrishnan
authors.affiliation: Department of Innate Immunity, National Institute for Research in Reproductive Health, Mumbai 400 012, India.
authors.forename: Hrishikesh
authors.initials: H
authors.lastname: Pandit
authors.affiliation: Centre for Infection, Immunity and Disease Mechanisms, College of Health and Life Science, Brunel University London, Uxbridge, UB8 3PH, United Kingdom.
authors.forename: Eswari Dodagatta-
authors.initials: ED
authors.lastname: Marri
authors.affiliation: Centre for Infection, Immunity and Disease Mechanisms, College of Health and Life Science, Brunel University London, Uxbridge, UB8 3PH, United Kingdom.
authors.forename: Lubna
authors.initials: L
authors.lastname: Kouser
authors.affiliation: Centre for Biotechnology and Bioinformatics, School of Life Sciences, Jawaharlal Nehru Institute for Advanced Studies, Secunderabad, Telangana, India.
authors.forename: Kaiser
authors.initials: K
authors.lastname: Jamil
authors.affiliation: Department of Infection and Immunity, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
authors.forename: Fatimah S
authors.initials: FS
authors.lastname: Alhamlan
authors.affiliation: Centre for Infection, Immunity and Disease Mechanisms, College of Health and Life Science, Brunel University London, Uxbridge, UB8 3PH, United Kingdom. Electronic address: uday.kishore@brunel.ac.uk.
authors.forename: Uday
authors.initials: U
authors.lastname: Kishore
authors.affiliation: Department of Innate Immunity, National Institute for Research in Reproductive Health, Mumbai 400 012, India. Electronic address: taruna_m@hotmail.com.
authors.forename: Taruna
authors.initials: T
authors.lastname: Madan
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000276:immunology; D003656:Decidua / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D005260:Female; D005333:Fetus; D005786:Gene Expression Regulation; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D061251:Primary Cell Culture; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D037663:Pulmonary Surfactant-Associated Protein D / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D000943:Antigens, Differentiation; D008070:Lipopolysaccharides; D037662:Pulmonary Surfactant-Associated Protein A; D037663:Pulmonary Surfactant-Associated Protein D; D014409:Tumor Necrosis Factor-alpha; C036763:monocyte-macrophage differentiation antigen
keywords: Decidua; Decidual macrophages; Murine; Parturition; Pregnancy; SP-A; SP-D; Stromal cells