title: Evaluation of solute penetration across the polysulfone membrane with vitamin E coating.
abstract: Vitamin E (vit E) is coated on polysulfone (PS) dialysis membrane for antioxidative purpose. The membrane, however, has not yet been evaluated from the mass transfer point of view. We investigated this PS membrane with and without vit E coating in vitro ultrafiltration experiments to identify whether or not the coating influences the mass transfer. Dialyzers that included PS membrane with and without vit E coating were investigated. Aqueous test solution of various solutes including vitamin B12 (VB12 , MW1355), chymotrypsin (chymo, MW25000), and albumin (alb, MW66000) was prepared, and normal ultrafiltration experiments were performed at 310 K. Reverse ultrafiltration experiments in which test solution was filtered from outside to inside the hollow fiber were also performed. Sieving coefficients (SC) were computed for evaluation. SC for VB12 was the same regardless of vit E coating; however, chymo was 0.82 ± 0.01 and 0.86 ± 0.01, respectively, for the membrane with and without vit E. Thus, it would be understood that vit E coating reduces the pore size of the membrane, resulting the reduced transport of larger solutes. Like other PS membrane, SC for alb was decreased over time regardless of vit E coating. More importantly, although the steady-state SC for alb was almost identical in two membranes, PS without vit E showed much greater decrease for the first 2 h, while that with vit E showed very little decrease over time, which suggested the reduced fouling effect due to vit E coating. All the SC values found in reverse ultrafiltration were higher than those found in normal ultrafiltration; moreover, the degree of increase with chymo was approximately 5%, whereas that with alb was approximately 430%, which may be explained by a new model in which wedge effect is taken into consideration for the membrane transport. Vit E coating not only has antioxidative effect but also reduces the fouling that is usually caused by various proteins.
authors.affiliation: Department of Chemical Science and Technology, Faculty of Bioscience and Applied Chemistry, Hosei University, Tokyo, Japan.
authors.forename: Akihiro C
authors.initials: AC
authors.lastname: Yamashita
authors.affiliation: Department of Chemical Science and Technology, Faculty of Bioscience and Applied Chemistry, Hosei University, Tokyo, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Masaki
authors.affiliation: Second Products Marketing Dept., Japanese Operation Division, Blood Purification Business Unit, Asahi Kasei Medical Co., Tokyo, Japan.
authors.forename: Eisuke
authors.initials: E
authors.lastname: Kobayashi
authors.affiliation: Blood Purification Development & Planning Dept, Asahi Kasei Medical MT Co., Tokyo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sukegawa
mesh_terms: D000975:Antioxidants; D006801:Humans; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry*; D006435:Renal Dialysis / Q000379:methods*; D013450:Sulfones / Q000737:chemistry*; D014810:Vitamin E / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; D014810:Vitamin E; C017662:polysulfone P 1700
keywords: Vitamin E; antioxidative; coating; dialysis membrane; α-tocopherol