title: Transcriptomic Analysis of Ovaries from Pigs with High And Low Litter Size.
abstract: Litter size is one of the most important economic traits for pig production as it is directly related to the production efficiency. Litter size is affected by interactions between multiple genes and the environment. While recent studies have identified some genes associated with prolificacy in pigs, transcriptomic studies of specific genes affecting litter size in porcine ovaries are rare. In order to identify candidate genes associated with litter size in swine, we assessed gene expression differences between the ovaries of Yorkshire pigs with extremely high and low litter sizes using the RNA-Seq method. A total of 1 243 differentially expressed genes were identified: 897 genes were upregulated and 346 genes were downregulated in high litter size ovary samples compared with low litter size ovary samples. A large number of these genes related to steroid hormone regulation in animal ovaries, including 59 Gene Ontology terms and 27 Kyoto Encyclopedia of Genes and Genomes pathways involved in steroid biosynthesis and ovarian steroidogenesis. From these differentially expressed genes, we identified a total of 11 genes using a bioinformatics screen that may be associated with high litter size in Yorkshire pigs. These results provide a list of new candidate genes for porcine litter size and prolificacy to be further investigated.
authors.affiliation: Key Laboratory of Local Animal Genetic Resources Conservation and Bio-breeding of Anhui province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui Province, People's Republic of China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Local Animal Genetic Resources Conservation and Bio-breeding of Anhui province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui Province, People's Republic of China.
authors.forename: Long
authors.initials: L
authors.lastname: Huang
authors.affiliation: Key Laboratory of Local Animal Genetic Resources Conservation and Bio-breeding of Anhui province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui Province, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.affiliation: Key Laboratory of Local Animal Genetic Resources Conservation and Bio-breeding of Anhui province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui Province, People's Republic of China.
authors.forename: Yifang
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Key Laboratory of Local Animal Genetic Resources Conservation and Bio-breeding of Anhui province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui Province, People's Republic of China.
authors.forename: Yueyun
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Key Laboratory of Local Animal Genetic Resources Conservation and Bio-breeding of Anhui province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui Province, People's Republic of China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Ye
authors.affiliation: Key Laboratory of Local Animal Genetic Resources Conservation and Bio-breeding of Anhui province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui Province, People's Republic of China.
authors.forename: Zongjun
authors.initials: Z
authors.lastname: Yin
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D019295:Computational Biology / Q000379:methods; D005260:Female; D020869:Gene Expression Profiling / Q000662:veterinary*; D063990:Gene Ontology; D059014:High-Throughput Nucleotide Sequencing / Q000662:veterinary*; D008098:Litter Size / Q000235:genetics*; D010053:Ovary / Q000378:metabolism*; D010641:Phenotype; D017423:Sequence Analysis, RNA / Q000662:veterinary*; D013552:Swine / Q000235:genetics*
chemical_list: D015415:Biomarkers