title: Effects of Bifidobacterium Breve Feeding Strategy and Delivery Modes on Experimental Allergic Rhinitis Mice.
abstract: BACKGROUND
  Different delivery modes may affect the susceptibility to allergic diseases. It is still unknown whether early intervention with probiotics would counteract this effect.
  
  
  OBJECTIVE
  The effect of different delivery modes on immune status and nasal symptoms was investigated on established allergic rhinitis (AR) mouse model. In addition, the immunoregulatory effects and mechanisms of different feeding manners with Bifidobacterium breve(B. breve) were examined.
  
  
  METHODS
  Live lyophilized B. breve was orally administered to BALB/c mice born via vaginal delivery(VD) or cesarean delivery (CD) for 8 consecutive weeks, after which they were sensitized by ovalbumin(OVA) to establish experimental AR. Nasal symptoms, serum immunoglobulins, cytokines, splenic percentages of CD4(+)CD25(+)Foxp3(+) regulatory T(Treg) cells and nasal eosinophil infiltration were evaluated.
  
  
  RESULTS
  Compared with VD mice, mice delivered via CD demonstrated more serious nasal symptoms, higher concentrations of OVA-specific immunoglobulin (Ig) E, more nasal eosinophils and lower percentages of splenic CD4(+)CD25(+)Foxp3(+)Treg cells after establishing experimental AR. These parameters were reversed by administering B. breves hortly after birth. However, the effect of B. breve did not differ between different delivery modes.
  
  
  CONCLUSIONS
  CD aggravates the nasal symptoms of AR mice compared to VD. This is the first report that oral administration of B. breve shortly after birth can significantly alleviate the symptoms of AR mice born via both deliveries, probably via activation of the regulatory capacity of CD4(+)CD25(+)Foxp3(+)Treg cells.
authors.affiliation: Department of Oto-Rhino-Laryngology West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Ren
authors.affiliation: Department of Oto-Rhino-Laryngology West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Oto-Rhino-Laryngology West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
authors.forename: Feng-Ling
authors.initials: FL
authors.lastname: Yang
authors.affiliation: Department of Oto-Rhino-Laryngology West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Lv
authors.affiliation: Department of Oto-Rhino-Laryngology West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Hung
authors.affiliation: Upper Airways Research Laboratory, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Upper Airways Research Laboratory, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Lin
authors.affiliation: Department of Oto-Rhino-Laryngology West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
authors.forename: Shi-Xi
authors.initials: SX
authors.lastname: Liu
authors.affiliation: Upper Airways Research Laboratory (URL), Department of Oto-Rhino-Laryngology, Ghent University, Ghent, Belgium.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Upper Airways Research Laboratory (URL), Department of Oto-Rhino-Laryngology, Ghent University, Ghent, Belgium.
authors.forename: Claus
authors.initials: C
authors.lastname: Bachert
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001644:Bifidobacterium / Q000276:immunology*; D015704:CD4 Antigens / Q000032:analysis / Q000276:immunology; D016207:Cytokines / Q000097:blood / Q000276:immunology; D005260:Female; D051858:Forkhead Transcription Factors / Q000032:analysis / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D056747:Immunomodulation; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics / Q000008:administration & dosage / Q000627:therapeutic use*; D065631:Rhinitis, Allergic / Q000097:blood / Q000276:immunology / Q000382:microbiology* / Q000628:therapy*; D012912:Sneezing; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000382:microbiology / Q000473:pathology
chemical_list: D015704:CD4 Antigens; D016207:Cytokines; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit; D007073:Immunoglobulin E