title: Combined treatment with parathyroid hormone (1-34) and beta-tricalcium phosphate had an additive effect on local bone formation in a rat defect model.
abstract: The objective of this study was to evaluate the effect of following combined treatment with parathyroid hormone (1-34) (PTH) and beta-tricalcium phosphate (β-TCP) on local bone formation in a rat 3-mm critical-size defect at distal femur. Twelve weeks after bilateral ovariectomy (OVX) and sham operation (sham), all animals were randomly divided into four groups: group OVX, group OVX + β-TCP, group sham, and group sham + β-TCP, then all rats underwent bone defect in the bilateral distal femurs, and β-TCP were implanted into critical-sized defects for group OVX + β-TCP and group sham + β-TCP. After defect operation, all animals were received following subcutaneous injections with PTH (60 μg/kg, three times a week) until death at 4 and 8 weeks. The defected area in distal femurs of rats was harvested for evaluation by histology, micro-CT, and biomechanics. The results of our study show that systemic usage of PTH or local usage of β-TCP can increase the healing of defects in OVX or sham rats. Furthermore, treatments with PTH and β-TCP showed a stronger effect on accelerating the local bone formation than used alone. Osteoporosis can limit the function of PTH and/or β-TCP. The results from our study demonstrate that combination of PTH and β-TCP had an additive effect on local bone formation in non-osteoporosis and/or osteoporosis rats.
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Xi Road, Wenzhou, 325027, Zhejiang, China.
authors.forename: Zhou-Shan
authors.initials: ZS
authors.lastname: Tao
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Xi Road, Wenzhou, 325027, Zhejiang, China.
authors.forename: Kai-Kai
authors.initials: KK
authors.lastname: Tu
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Xi Road, Wenzhou, 325027, Zhejiang, China.
authors.forename: Zheng-Liang
authors.initials: ZL
authors.lastname: Huang
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Xi Road, Wenzhou, 325027, Zhejiang, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Xi Road, Wenzhou, 325027, Zhejiang, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Xi Road, Wenzhou, 325027, Zhejiang, China.
authors.forename: Hong-Ming
authors.initials: HM
authors.lastname: Xu
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Xi Road, Wenzhou, 325027, Zhejiang, China.
authors.forename: Yu-Long
authors.initials: YL
authors.lastname: Zhou
authors.affiliation: Department of Orthopaedic Surgery, Wenzhou Central Hospital, Wenzhou, 325000, Zhejiang, China.
authors.forename: Yang-Xun
authors.initials: YX
authors.lastname: Lv
authors.affiliation: Sichuan Provincial Orthopedics Hospital, NO. 132 West First Section First Ring Road, Chengdu, 610000, Sichuan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Xi Road, Wenzhou, 325027, Zhejiang, China. tzs19900327@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
mesh_terms: D000818:Animals; D015519:Bone Density; D002130:Calcium Phosphates / Q000494:pharmacology*; D000071556:Cancellous Bone / Q000187:drug effects / Q000503:physiopathology / Q000648:ultrastructure; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D005260:Female; D005269:Femur / Q000187:drug effects / Q000503:physiopathology; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D010024:Osteoporosis / Q000209:etiology / Q000503:physiopathology*; D010052:Ovariectomy; D010281:Parathyroid Hormone / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D055114:X-Ray Microtomography
chemical_list: D002130:Calcium Phosphates; D010281:Parathyroid Hormone; C485817:beta-tricalcium phosphate
keywords: Defect healing; Micro-CT; Osteoporotic bone; Parathyroid hormone; β-Tricalcium phosphate