title: The Development of TALE Nucleases for Biotechnology.
abstract: The development of a facile genome engineering technology based on transcription activator-like effector nucleases (TALENs) has led to significant advances in diverse areas of science and medicine. In this review, we provide a broad overview of the development of TALENs and the use of this technology in basic science, biotechnology, and biomedical applications. This includes the discovery of DNA recognition by TALEs, engineering new TALE proteins to diverse targets, general advances in nuclease-based editing strategies, and challenges that are specific to various applications of the TALEN technology. We review examples of applying TALENs for studying gene function and regulation, generating disease models, and developing gene therapies. The current status of genome editing and future directions for other uses of these technologies are also discussed.
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC, 27708, USA.
authors.forename: David G
authors.initials: DG
authors.lastname: Ousterout
authors.affiliation: Department of Biomedical Engineering, Duke University, Room 136 Hudson Hall, Box 90281, Durham, NC, 27708-0281, USA. charles.gersbach@duke.edu.|Center for Genomic and Computational Biology, Duke University, Durham, NC, 27708, USA. charles.gersbach@duke.edu.|Department of Orthopaedic Surgery, Duke University Medical Center, Durham, NC, 27710, USA. charles.gersbach@duke.edu.
authors.forename: Charles A
authors.initials: CA
authors.lastname: Gersbach
mesh_terms: D000818:Animals; D003851:Deoxyribonucleases / Q000235:genetics; D018390:Gene Targeting / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods*; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D017393:RNA Editing / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*
chemical_list: D018398:Homeodomain Proteins; D012097:Repressor Proteins; C490797:Tgif1 protein, mouse; D003851:Deoxyribonucleases
keywords: Gene editing; Gene targeting; Gene therapy; Genome engineering