title: Positive skeletal effect of two ingredients of Psoralea corylifolia L. on estrogen deficiency-induced osteoporosis and the possible mechanisms of action.
abstract: Estrogen replacement therapy (ERT) is utilized as a major regime for treatment of postmenopausal osteoporosis at present. However, long-term supplement of estrogen may cause uterine hyperplasia and hypertension leading to a high risk of endometrial cancer and breast cancer. Psoralea corylifolia L. has long been used as tonic and food additives in many countries. Previous studies had found two ingredients in P. corylifolia L.: bavachin and bakuchiol exhibited osteoblastic activity. The present study was designed to investigate the protective effect of bakuchiol and bavachin on ovariectomy-induced bone loss and explore the possible mechanism. In vivo, bakuchiol and bavachin could prevented estrogen deficiency-induced bone loss in ovariectomized rats without uterotrophic activity. In vitro studies suggested that bakuchiol and bavachin induced primary human osteoblast differentiation by up-regulating the Wnt signalling pathway. This study suggests that such a bone-protective role makes them a promising and safe estrogen supplement for the ERT.
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210046, China; School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, 211198, China.
authors.forename: Ze-Bin
authors.initials: ZB
authors.lastname: Weng
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210046, China; Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing, 210046, China.
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Gao
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210046, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210046, China; Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing, 210046, China.
authors.forename: Gen-Hua
authors.initials: GH
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210046, China; Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing, 210046, China.
authors.forename: Fang-Zhou
authors.initials: FZ
authors.lastname: Yin
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing, 210046, China.
authors.forename: Bao-Chang
authors.initials: BC
authors.lastname: Cai
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210046, China; Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing, 210046, China. Electronic address: cpuczp2000@hotmail.com.
authors.forename: Zhi-Peng
authors.initials: ZP
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210046, China; Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing, 210046, China. Electronic address: liweidong0801@163.com.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Li
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D005419:Flavonoids / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010010:Osteoclasts / Q000187:drug effects; D010024:Osteoporosis / Q000188:drug therapy* / Q000209:etiology; D010052:Ovariectomy / Q000009:adverse effects*; D010636:Phenols / Q000008:administration & dosage* / Q000494:pharmacology; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D029907:Psoralea / Q000737:chemistry*; D051381:Rats; D015854:Up-Regulation; D060449:Wnt Signaling Pathway / Q000187:drug effects
chemical_list: D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; C459212:bavachin; C012765:bakuchiol
keywords: 17-β-estradiol (PubChem CID: 5757); Bakuchiol; Bakuchiol (PubChem CID: 5468522); Bavachin; Bavachin (PubChem CID: 5321775); Dimethyl sulfoxid (PubChem CID: 679); Methyl thiazolyl tetrazolium (PubChem CID: 16218671); Osteoblast; Postmenopausal osteoporosis; Wnt signalling pathway