title: Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries.
abstract: Atherosclerosis is characterized by endothelial dysfunction and alterations in vascular reactivity, which can be investigated by wire myography. The method allows ex vivo monitoring of the transversal isometric tension developed by a vessel segment in response to different pathophysiological stimuli. Here we describe in detail how to use the wire myograph to evaluate endothelial function and vasoconstrictor or vasodilator properties of the vessel, as well as to identify and characterize different factors and molecular pathways that control vascular tone. We also describe how to use the wire myograph to analyze biomechanical and passive properties of vessels such as diameter and elasticity.
authors.affiliation: Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Vascular Biology Program, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029, Madrid, Spain. lara.delcampo@cnic.es.
authors.forename: Lara
authors.initials: L
authors.lastname: del Campo
authors.affiliation: Department of Physiology, Faculty of Medicine, Universidad Autónoma de Madrid, Arzobispo Morcillo 4, 28029, Madrid, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Ferrer
mesh_terms: D000818:Animals; D001158:Arteries / Q000187:drug effects / Q000502:physiology*; D001696:Biomechanical Phenomena; D004210:Dissection; D004548:Elasticity; D004558:Electric Stimulation; D066298:In Vitro Techniques; D051379:Mice; D009213:Myography / Q000295:instrumentation / Q000379:methods*; D011312:Pressure; D012815:Signal Processing, Computer-Assisted; D013997:Time Factors; D059289:Vascular Stiffness; D014661:Vasoconstriction / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents
keywords: Endothelial function; Isolated mouse arteries; Vascular reactivity; Vascular structure; Vascular tone; Wire myograph