title: Production of autoinducer-2 by aerobic endospore-forming bacteria isolated from the West African fermented foods.
abstract: Autoinducer-2 (AI-2) is a quorum-sensing (QS) molecule which mediates interspecies signaling and affects various bacterial behaviors in food fermentation. Biosynthesis of AI-2 is controlled by S-ribosylhomocysteine lyase encoded by the luxS gene. The objective of this study was to investigate production of AI-2 by aerobic endospore-forming bacteria (AEB) isolated from the West African alkaline fermented seed products Mantchoua and Maari. The study included 13 AEB strains of Bacillus subtilis, B. cereus, B. altitudinis, B. amyloliquefaciens, B. licheniformis, B. aryabhattai, B. safensis, Lysinibacillus macroides and Paenibacillus polymyxa. All the tested strains harbored the luxS gene and all strains except for P. polymyxa B314 were able to produce AI-2 during incubation in laboratory medium. Production of AI-2 by AEB was growth phase dependent, showing maximum activity at the late exponential phase. AI-2 was depleted from the culture medium at the beginning of the stationary growth phase, indicating that the tested AEB possess a functional AI-2 receptor that internalizes AI-2. This study provides the evidences of QS system in Bacillus spp. and L. macroides and new knowledge of AI-2 production by AEB. This knowledge contributes to the development of QS-based strategies for better control of alkaline fermentation.
authors.affiliation: Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 26, 1958 Frederiksberg C, Denmark yangqian@food.ku.dk.
authors.forename: Yang
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Département Technologie Alimentaire, Université Polytechnique de Bobo-Dioulasso, 01 BP 1091 Bobo-Dioulasso, Burkina Faso.
authors.forename: Christine Kere
authors.initials: CK
authors.lastname: Kando
authors.affiliation: Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 26, 1958 Frederiksberg C, Denmark.
authors.forename: Line
authors.initials: L
authors.lastname: Thorsen
authors.affiliation: Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 26, 1958 Frederiksberg C, Denmark.
authors.forename: Nadja
authors.initials: N
authors.lastname: Larsen
authors.affiliation: Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 26, 1958 Frederiksberg C, Denmark.
authors.forename: Lene
authors.initials: L
authors.lastname: Jespersen
mesh_terms: D000354:Africa, Western; D001406:Bacillaceae / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001420:Bacteria, Aerobic / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D001692:Biological Transport; D013437:Carbon-Sulfur Lyases / Q000235:genetics; D003470:Culture Media / Q000737:chemistry; D004725:Endospore-Forming Bacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D005516:Food Microbiology; D006714:Homoserine / Q000031:analogs & derivatives* / Q000378:metabolism; D007783:Lactones / Q000378:metabolism*; D056507:Paenibacillus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D053038:Quorum Sensing
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria
keywords: Lsr system; alkaline fermentations; luxS gene; quorum sensing