title: Binding properties of drospirenone with human serum albumin and lysozyme in vitro.
abstract: The interaction of drospirenone (DP) with human serum albumin (HSA)/lysozyme (LYZ) was investigated using different optical techniques and molecular models. Results from the emission and time resolved fluorescence studies revealed that HSA/LYZ emission quenching with DP was initiated by static quenching mechanism. The LYZ-DP system was more easily influenced by temperature than the HSA-DP system. Displacement experiments demonstrated that the DP binding site was mainly located in site 1 of HSA. Based on the docking methods, DP was mainly bound in the active site hinge region where Trp-62 and Trp-63 are located. Conformation study showed that DP had different effects on the local conformation of HSA and LYZ molecules.
authors.affiliation: College of Chemical Engineering, Sichuan University, Chengdu 610065, Sichuan, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Chemical Engineering, Sichuan University, Chengdu 610065, Sichuan, China.
authors.forename: Xiangling
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Chemical Engineering, Sichuan University, Chengdu 610065, Sichuan, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: He
authors.affiliation: College of Chemical Engineering, Sichuan University, Chengdu 610065, Sichuan, China.
authors.forename: Qiaomei
authors.initials: Q
authors.lastname: Sun
authors.affiliation: College of Chemical Engineering, Sichuan University, Chengdu 610065, Sichuan, China.
authors.forename: Yuanzhi
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Chemical Engineering, Sichuan University, Chengdu 610065, Sichuan, China. Electronic address: lihuilab@sina.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
mesh_terms: D000736:Androstenes / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D020134:Catalytic Domain; D002645:Chickens; D002942:Circular Dichroism; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D009113:Muramidase / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013997:Time Factors
chemical_list: D000736:Androstenes; D012709:Serum Albumin; D009113:Muramidase; C035144:drospirenone
keywords: Drospirenone; Human serum albumin (HSA); Lysozyme (LYZ); Main binding site; Molecular modeling