title: Tight junction disruption of blood-brain barrier in white matter lesions in chronic hypertensive rats.
abstract: Tight junctions (TJs) are the most important structure of the blood-brain barrier (BBB). Studies have shown that triggering of white matter lesions (WMLs) may be related to a BBB dysfunction, but rarely have studies observed the progressive changes in TJs longitudinally. In our present study, the ultrastructure of TJs was observed using a transmission electron microscope in Stroke-prone Renalvascular Hypertensive Rats. Western blotting was used to detect TJ-related proteins zonula occludens-1 and occludin. The results showed that in Stroke-prone Renalvascular Hypertensive Rats, the severity of WMLs increased gradually. TJs was destroyed gradually 8 weeks after hypertension. The levels of zonula occludens-1 and occludin also decreased gradually. These data suggested that long-term hypertension may contribute toward the gradual disruption of TJs of BBB and induce WMLs in chronic hypertensive rats.
authors.affiliation: aDepartment of Neurology, First Affiliated Hospital of Sun Yat-sen University bDepartment of Clinical Research, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou cDepartment of Neurology, Fujian Province Hospital, Fuzhou, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Fan
authors.forename: Xian
authors.initials: X
authors.lastname: Yang
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Tao
authors.forename: Linfang
authors.initials: L
authors.lastname: Lan
authors.forename: Lu
authors.initials: L
authors.lastname: Zheng
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
mesh_terms: D000818:Animals; D001794:Blood Pressure; D001812:Blood-Brain Barrier / Q000648:ultrastructure*; D002908:Chronic Disease; D006973:Hypertension / Q000378:metabolism / Q000473:pathology*; D008297:Male; D062793:Occludin / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D019108:Tight Junctions / Q000648:ultrastructure*; D066127:White Matter / Q000473:pathology*; D062826:Zonula Occludens-1 Protein / Q000378:metabolism
chemical_list: D062793:Occludin; C568987:Ocln protein, rat; D062826:Zonula Occludens-1 Protein