title: DNA microarray analysis of the cyanotroph Pseudomonas pseudoalcaligenes CECT5344 in response to nitrogen starvation, cyanide and a jewelry wastewater.
abstract: Pseudomonas pseudoalcaligenes CECT5344 is an alkaliphilic bacterium that can use cyanide as nitrogen source for growth, becoming a suitable candidate to be applied in biological treatment of cyanide-containing wastewaters. The assessment of the whole genome sequence of the strain CECT5344 has allowed the generation of DNA microarrays to analyze the response to different nitrogen sources. The mRNA of P. pseudoalcaligenes CECT5344 cells grown under nitrogen limiting conditions showed considerable changes when compared against the transcripts from cells grown with ammonium; up-regulated genes were, among others, the glnK gene encoding the nitrogen regulatory protein PII, the two-component ntrBC system involved in global nitrogen regulation, and the ammonium transporter-encoding amtB gene. The protein coding transcripts of P. pseudoalcaligenes CECT5344 cells grown with sodium cyanide or an industrial jewelry wastewater that contains high concentration of cyanide and metals like iron, copper and zinc, were also compared against the transcripts of cells grown with ammonium as nitrogen source. This analysis revealed the induction by cyanide and the cyanide-rich wastewater of four nitrilase-encoding genes, including the nitC gene that is essential for cyanide assimilation, the cyanase cynS gene involved in cyanate assimilation, the cioAB genes required for the cyanide-insensitive respiration, and the ahpC gene coding for an alkyl-hydroperoxide reductase that could be related with iron homeostasis and oxidative stress. The nitC and cynS genes were also induced in cells grown under nitrogen starvation conditions. In cells grown with the jewelry wastewater, a malate quinone:oxidoreductase mqoB gene and several genes coding for metal extrusion systems were specifically induced.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, 1ª Planta, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain.
authors.forename: V M
authors.initials: VM
authors.lastname: Luque-Almagro
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, 1ª Planta, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain.
authors.forename: M P
authors.initials: MP
authors.lastname: Escribano
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, 1ª Planta, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Manso
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, 1ª Planta, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain.
authors.forename: L P
authors.initials: LP
authors.lastname: Sáez
authors.affiliation: Departamento de Botánica, Ecología y Fisiología Vegetal, Edificio Celestino Mutis, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Cabello
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, 1ª Planta, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Moreno-Vivián
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, 1ª Planta, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain. Electronic address: bb2rorum@uco.es.
authors.forename: M D
authors.initials: MD
authors.lastname: Roldán
mesh_terms: D003486:Cyanides / Q000633:toxicity*; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D007220:Industrial Waste; D055498:Jewelry; D009584:Nitrogen / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D044205:Pseudomonas pseudoalcaligenes / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D062065:Wastewater / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity; D018508:Water Purification
chemical_list: D003486:Cyanides; D004269:DNA, Bacterial; D007220:Industrial Waste; D009584:Nitrogen; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Cyanide degradation; Jewelry wastewater; Microarrays; Nitrogen starvation; Pseudomonas