title: Expression and Function of Hypoxia Inducible Factor-1α and Vascular Endothelial Growth Factor in Pulp Tissue of Teeth under Orthodontic Movement.
abstract: Orthodontic force may lead to cell damage, circulatory disturbances, and vascular changes of the dental pulp, which make a hypoxic environment in pulp. In order to maintain the homeostasis of dental pulp, hypoxia will inevitably induce the defensive reaction. However, this is a complex process and is regulated by numerous factors. In this study, we established an experimental animal model of orthodontic tooth movement to investigate the effects of mechanical force on the expression of VEGF and HIF-1α in dental pulp. Histological analysis of dental pulp and expressions of HIF-1α and VEGF proteins in dental pulp were examined. The results showed that inflammation and vascular changes happened in dental pulp tissue in different periods. Additionally, there were significant changes in the expression of HIF-1α and VEGF proteins under orthodontic force. After application of mechanical load, expression of HIF-1α and VEGF was markedly positive in 1, 3, 7 d, and 2 w groups, and then it weakened in 4 w group. These findings suggested that the expression of HIF-1α and VEGF was enhanced by mechanical force. HIF-1α and VEGF may play an important role in retaining the homeostasis of dental pulp during orthodontic tooth movement.
authors.affiliation: Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong, China ; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, No. 44, Wen Hua Xi Lu, Shandong, Jinan 250012, China.
authors.forename: Fulan
authors.initials: F
authors.lastname: Wei
authors.affiliation: Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong, China ; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, No. 44, Wen Hua Xi Lu, Shandong, Jinan 250012, China.
authors.forename: Shuangyan
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing 100050, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong, China ; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, No. 44, Wen Hua Xi Lu, Shandong, Jinan 250012, China.
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong, China ; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, No. 44, Wen Hua Xi Lu, Shandong, Jinan 250012, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong, China ; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, No. 44, Wen Hua Xi Lu, Shandong, Jinan 250012, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Hu
authors.affiliation: Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong, China ; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, No. 44, Wen Hua Xi Lu, Shandong, Jinan 250012, China.
authors.forename: Dongxu
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong, China ; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, No. 44, Wen Hua Xi Lu, Shandong, Jinan 250012, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D003782:Dental Pulp / Q000378:metabolism*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D042461:Vascular Endothelial Growth Factor A