title: Decreased expression of long non-coding RNA WT1-AS promotes cell proliferation and invasion in gastric cancer.
abstract: Tumor recurrence and metastasis remain the major obstacles for the successful treatment of patients diagnosed with gastric cancer. In recent years, long non-coding RNAs (lncRNAs) have been considered as key regulators of tumor behavior. In this study, we investigated the expression and biological role of a newly-identified cancer-related lncRNA, WT1-AS. We found that WT1-AS expression was significantly down-regulated in tumor tissues compared to matched adjacent non-tumor tissues. The WT1-AS expression level was also associated with tumor size and the clinicopathological stage. Cell proliferation, migration, and invasion were inhibited, and the proportion of G0/G1 cells increased when WT1-AS was ectopically-expressed in gastric cancer cells. Furthermore, ectopic expression of WT1-AS was demonstrated to inhibit tumor growth and metastasis in vivo. Finally, we found that WT1-AS overexpression could decrease ERK protein phosphorylation. Our study indicates that WT1-AS is significantly down-regulated in gastric cancers and may be correlated with tumor progression.
authors.affiliation: Department of Gastrointestinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, No 150 Jimo Road, Shanghai, China. Electronic address: sfgidt1982@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Du
authors.affiliation: Affiliated Hospital of Jining Medical University, Jining 272000, China; Shanghai Key laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Department of Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of medicine, No 197 Ruijin er Road, Shanghai 200025, China. Electronic address: zyjzbg@126.com.
authors.forename: Baogui
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Gastrointestinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, No 150 Jimo Road, Shanghai, China. Electronic address: v2zs@hotmail.com.
authors.forename: Shun
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Gastrointestinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, No 150 Jimo Road, Shanghai, China. Electronic address: jxh@medmail.com.cn.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Gastrointestinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, No 150 Jimo Road, Shanghai, China. Electronic address: zp1377@easthospital.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Zheng
authors.affiliation: Shanghai Key laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Department of Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of medicine, No 197 Ruijin er Road, Shanghai 200025, China. Electronic address: rjljf@163.com.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Li
authors.affiliation: Shanghai Key laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Department of Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of medicine, No 197 Ruijin er Road, Shanghai 200025, China. Electronic address: ym10299@163.com.
authors.forename: Min
authors.initials: M
authors.lastname: Yan
authors.affiliation: Shanghai Key laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Department of Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of medicine, No 197 Ruijin er Road, Shanghai 200025, China. Electronic address: zzg1954@hotmail.com.
authors.forename: Zhenggang
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Shanghai Key laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Department of Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of medicine, No 197 Ruijin er Road, Shanghai 200025, China. Electronic address: liubingya@sjtu.edu.cn.
authors.forename: Bingya
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D010766:Phosphorylation; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding; C000606336:WT1-as long non-coding RNA, human; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: Gastric cancer; LncRNA; Progression; Proliferation; WT1-AS