title: Analysis of Altered Micro RNA Expression Profiles in Focal Cortical Dysplasia IIB.
abstract: Focal cortical dysplasia type IIB is a commonly encountered subtype of developmental malformation of the cerebral cortex and is often associated with pharmacoresistant epilepsy. In this study, to investigate the molecular etiology of focal cortical dysplasia type IIB, the authors performed micro ribonucleic acid (RNA) microarray on surgical specimens from 5 children (2 female and 3 male, mean age was 73.4 months, range 50-112 months) diagnosed of focal cortical dysplasia type IIB and matched normal tissue adjacent to the lesion. In all, 24 micro RNAs were differentially expressed in focal cortical dysplasia type IIB, and the microarray results were validated using quantitative real-time polymerase chain reaction (PCR). Then the putative target genes of the differentially expressed micro RNAs were identified by bioinformatics analysis. Moreover, biological significance of the target genes was evaluated by investigating the pathways in which the genes were enriched, and the Hippo signaling pathway was proposed to be highly related with the pathogenesis of focal cortical dysplasia type IIB.
authors.affiliation: Department of Functional Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Functional Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, China.
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Liu
authors.affiliation: Department of Neurology, Sanbo Brain Hospital, Capital Medical University, Beijing, China Beijing Key Laboratory in Epilepsy, Beijing, China Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing, China.
authors.forename: Tian-Fu
authors.initials: TF
authors.lastname: Li
authors.affiliation: Department of Functional Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, China.
authors.forename: Yu-Guang
authors.initials: YG
authors.lastname: Guan
authors.affiliation: Department of Functional Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Functional Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, China.
authors.forename: Xue-Ling
authors.initials: XL
authors.lastname: Qi
authors.affiliation: Beijing Key Laboratory of Neural Regeneration and Repair, Department of Neurobiology, Beijing, China.
authors.forename: Yu-Tao
authors.initials: YT
authors.lastname: Yang
authors.affiliation: Beijing Key Laboratory in Epilepsy, Beijing, China.
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Deng
authors.affiliation: Beijing Key Laboratory of Neural Regeneration and Repair, Department of Neurobiology, Beijing, China Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
authors.forename: Zhi-Qing David
authors.initials: ZQ
authors.lastname: Xu
authors.affiliation: Department of Functional Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, China Beijing Key Laboratory in Epilepsy, Beijing, China Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing, China luangm666@163.com.
authors.forename: Guo-Ming
authors.initials: GM
authors.lastname: Luan
mesh_terms: D064229:Biological Ontologies; D001921:Brain / Q000000981:diagnostic imaging; D002648:Child; D002675:Child, Preschool; D019295:Computational Biology; D004827:Epilepsy / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging; D008297:Male; D065703:Malformations of Cortical Development, Group I / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D035683:MicroRNAs
keywords: Hippo signaling pathway; focal cortical dysplasia type IIB; micro RNA