title: SERS substrates fabricated using ceramic filters for the detection of bacteria.
abstract: SERS substrates were fabricated by filtering either Ag or Au colloidal particles onto rigid, ceramic filters - onto which suspensions of bacteria were then filtered. SERS spectra of the bacteria were obtained using a Raman spectrometer that has an 'orbital raster scan' capability. It was shown that bacteria samples prepared in this manner were uniformly distributed onto the surface of the SERS substrate. The effect of common buffer systems on the SERS spectra was investigated and the utility of using the SERS technique for speciation of bacteria was explored.
authors.affiliation: SPAWAR Systems Center Pacific, San Diego, CA 92152. Electronic address: pam.boss@navy.mil.
authors.forename: P A
authors.initials: PA
authors.lastname: Mosier-Boss
authors.affiliation: SPAWAR Systems Center Pacific, San Diego, CA 92152.
authors.forename: K C
authors.initials: KC
authors.lastname: Sorensen
authors.affiliation: SPAWAR Systems Center Pacific, San Diego, CA 92152.
authors.forename: R D
authors.initials: RD
authors.lastname: George
authors.affiliation: San Diego State University Foundation, San Diego, CA 92182.
authors.forename: A
authors.initials: A
authors.lastname: Obraztsova
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D002516:Ceramics / Q000737:chemistry*; D003102:Colloids / Q000737:chemistry; D004926:Escherichia coli / Q000302:isolation & purification; D005374:Filtration / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry; D007834:Lasers; D044224:Pseudomonas syringae / Q000302:isolation & purification; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D003102:Colloids; D012834:Silver; D006046:Gold
keywords: SERS; bacteria; ceramic filter; orbital raster scan