title: Genome sequence of a cluster A13 mycobacteriophage detected in Mycobacterium phlei over a half century ago.
abstract: A phage infecting Mycobacterium phlei was isolated in 1958 from a soil sample in Hungary. Some physicochemical and biological properties of the virus were described in independent studies over the years. Here, we report the genome sequence of this early mycobacteriophage isolate. The Phlei phage genome measured 50,418 bp, had a GC content of 60.1 % and was predicted to encode 81 proteins and three tRNAs. Phylogeny of the tape measure protein revealed genetic relatedness to other early isolates of mycobacteriophages within subcluster A2. The genomic organization and genetic relationships to other strains showed that the Phlei phage belongs to a novel genetic cluster, designated A13.
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungaria krt 21, 1143, Budapest, Hungary.
authors.forename: Szilvia
authors.initials: S
authors.lastname: Marton
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungaria krt 21, 1143, Budapest, Hungary.
authors.forename: Enikő
authors.initials: E
authors.lastname: Fehér
authors.affiliation: Biological Research Centre, Hungarian Academy of Sciences, Temesvari krt. 62, 6726, Szeged, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Horváth
authors.affiliation: Microbiological Research Group, National Center for Epidemiology, Piheno u. 1, 1529, Budapest, Hungary.
authors.forename: Katalin
authors.initials: K
authors.lastname: Háber
authors.affiliation: Microbiological Research Group, National Center for Epidemiology, Piheno u. 1, 1529, Budapest, Hungary.
authors.forename: Pál
authors.initials: P
authors.lastname: Somogyi
authors.affiliation: Microbiological Research Group, National Center for Epidemiology, Piheno u. 1, 1529, Budapest, Hungary.|Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Tisza Lajos krt. 64., 6720, Szeged, Hungary.
authors.forename: János
authors.initials: J
authors.lastname: Minárovits
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungaria krt 21, 1143, Budapest, Hungary. bkrota@hotmail.com.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Bányai
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009160:Mycobacteriophages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D009168:Mycobacterium phlei / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny