title: Genetic Methods for Cellular Manipulations in C. elegans.
abstract: Neuron manipulation in vivo by ablation, activation, or inactivation, and regulation of gene expression, is essential for dissecting nervous system function. Here we describe genetic means for neuron manipulation in the nematode C. elegans, and provide protocols for generating transgenic animals containing these genetic tools.
authors.affiliation: Laboratory of Developmental Genetics, The Rockefeller University, 810 Weiss Research Building, 1230 York Avenue, New York, NY, 10065, USA. mkatz@rockefeller.edu.
authors.forename: Menachem
authors.initials: M
authors.lastname: Katz
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics*
keywords: C. elegans; Cell-ablation; Microinjection; Neuronal-activation; Neuronal-silencing; Optogenetic; Transgenic worms