title: Kinetics of hematopoietic stem cells and supportive activities of stromal cells in a three-dimensional bone marrow culture system.
abstract: In the bone marrow, hematopoietic cells proliferate and differentiate in close association with a three-dimensional (3D) hematopoietic microenvironment. Previously, we established a 3D bone marrow culture system. In this study, we analyzed the kinetics of hematopoietic cells, and more than 50% of hematopoietic progenitor cells, including CFU-Mix, CFU-GM and BFU-E in 3D culture were in a resting (non-S) phase. Furthermore, we examined the hematopoietic supportive ability of stromal cells by measuring the expression of various mRNAs relevant to hematopoietic regulation. Over the 4 weeks of culture, the stromal cells in the 3D culture are not needlessly activated and "quietly" regulate hematopoietic cell proliferation and differentiation during the culture, resulting in the presence of resting hematopoietic stem cells in the 3D culture for a long time. Thus, the 3D culture system may be a new tool for investigating hematopoietic stem cell-stromal cell interactions in vitro.
authors.affiliation: a Department of Functional Morphology and.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Harada
authors.affiliation: a Department of Functional Morphology and.|b Department of Medicine , Nihon University School of Medicine , Tokyo , Japan .
authors.forename: Yukio
authors.initials: Y
authors.lastname: Hirabayashi
authors.affiliation: b Department of Medicine , Nihon University School of Medicine , Tokyo , Japan .
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hatta
authors.affiliation: a Department of Functional Morphology and.
authors.forename: Isao
authors.initials: I
authors.lastname: Tsuboi
authors.affiliation: c Department of Chemical Engineering , Norwegian University of Science and Technology , Trondheim , Norway , and.
authors.forename: Wilhelm Robert
authors.initials: WR
authors.lastname: Glomm
authors.affiliation: d Department of Chemical Engineering , Osaka Prefecture University , Osaka , Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yasuda
authors.affiliation: a Department of Functional Morphology and.
authors.forename: Shin
authors.initials: S
authors.lastname: Aizawa
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D002453:Cell Cycle / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger
keywords: 3D culture; bone marrow stromal cell; cytokines; polymer particles; stem cell