title: Identification of a conserved neutralizing linear B-cell epitope in the VP1 proteins of duck hepatitis A virus type 1 and 3.
abstract: Duck virus hepatitis (DVH), mainly caused by duck hepatitis A virus (DHAV), is a severe disease threaten to duck industry and has worldwide distribution. As the major structural protein, the VP1 protein of DHAV is able to induce neutralizing antibody in ducks. In this study, a monoclonal antibody (mAb) 4F8 against the intact DHAV-1 particles was used to identify the possible epitope in the three serotypes of DHAV. The mAb 4F8 had weak neutralizing activities to both DHAV-1 and DHAV-3, and reacted with the conserved linear B-cell epitopes of (75)GEIILT(80) in DHAV-1 VP1 and (75)GEVILT(80) in DHAV-3 VP1 protein, respectively, while not with DHAV-2 VP1. This was the first report about identification of the common conserved neutralizing linear B-cell epitope of DHAV-1 and DHAV-3, which will facilitate understanding of the antigenic structure of VP1 and the serologic diagnosis of DHAV infection.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, Taian 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Taian, 271018, China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, Taian 271018, China.
authors.forename: Guomei
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, Taian 271018, China.
authors.forename: Yinghao
authors.initials: Y
authors.lastname: Xin
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, Taian 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Taian, 271018, China.
authors.forename: Junhao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, Taian 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Taian, 271018, China.
authors.forename: Shaoli
authors.initials: S
authors.lastname: Lin
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, Taian 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Taian, 271018, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, Taian 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Taian, 271018, China.
authors.forename: Zhijing
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, Taian 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Taian, 271018, China. Electronic address: sjjiang@sdau.edu.cn.
authors.forename: Shijin
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000276:immunology; D000956:Antigens, Viral / Q000737:chemistry* / Q000276:immunology; D004372:Ducks / Q000821:virology*; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D004371:Hepatitis Virus, Duck / Q000276:immunology*; D006524:Hepatitis, Viral, Animal / Q000821:virology; D008969:Molecular Sequence Data; D010850:Picornaviridae Infections / Q000662:veterinary / Q000821:virology; D011201:Poultry Diseases / Q000821:virology; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000956:Antigens, Viral; D018985:Epitopes, B-Lymphocyte; C096539:VP1 protein, hepatitis A virus; D015678:Viral Structural Proteins
keywords: Duck hepatitis A virus 1 (DHAV-1); Duck hepatitis A virus 3 (DHAV-3); Epitope; Monoclonal antibody (mAb)