title: Non-Fouling Biodegradable Poly(ϵ-caprolactone) Nanofibers for Tissue Engineering.
abstract: Poly(ϵ-caprolactone) (PCL) nanofibers are very attractive materials for tissue engineering (TE) due to their degradability and structural similarity to the extracellular matrix (ECM). However, upon exposure to biological media, their surface is rapidly fouled by proteins and cells, which may lead to inflammation and foreign body reaction. In this study, an approach for the modification of PCL nanofibers to prevent protein fouling from biological fluids and subsequent cell adhesion is introduced. A biomimetic polydopamine (PDA) layer was deposited on the surface of the PCL nanofibers and four types of antifouling polymer brushes were grown by surface-initiated atom transfer radical polymerization (SI-ATRP) from initiator moieties covalently attached to the PDA layer. Cell adhesion was assessed with mouse embryonic fibroblasts (MEFs). MEFs rapidly adhered and formed cell-matrix adhesions (CMAs) with PCL and PCL-PDA nanofibers. Importantly, the nanofibers modified with antifouling polymer brushes were able to suppress non-specific protein adsorption and thereby cell adhesion.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovsky sq.2, Prague 162 06, Czech Republic.
authors.forename: Nina Yu
authors.initials: NY
authors.lastname: Kostina
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovsky sq.2, Prague 162 06, Czech Republic.
authors.forename: Ognen
authors.initials: O
authors.lastname: Pop-Georgievski
authors.affiliation: Zoological Institute, Cell and Neurobiology, Karlsruhe Institute of Technology (KIT), Haid-und-Neu-Straße 9, Karlsruhe 76131, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Bachmann
authors.affiliation: Institute of Physics, Academy of Sciences of the Czech Republic, Cukrovarnicka 10, Prague 16253, Czech Republic.|Faculty of Nuclear Science and Physical Engineering, Czech Technical University in Prague, Trojanova 13, Prague 12000, Czech Republic.
authors.forename: Neda
authors.initials: N
authors.lastname: Neykova
authors.affiliation: Institute for Applied Materials (IAM) and Karlsruhe Nano Micro Facility (KNMF), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, Eggenstein-Leopoldshafen 76344, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Bruns
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovsky sq.2, Prague 162 06, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Michálek
authors.affiliation: Zoological Institute, Cell and Neurobiology, Karlsruhe Institute of Technology (KIT), Haid-und-Neu-Straße 9, Karlsruhe 76131, Germany. martin.bastmeyer@kit.edu.|Institute for Functional Interfaces (IFG) Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, Eggenstein-Leopoldshafen 76344, Germany. martin.bastmeyer@kit.edu.
authors.forename: Martin
authors.initials: M
authors.lastname: Bastmeyer
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovsky sq.2, Prague 162 06, Czech Republic. rodriguez@imc.cas.cz.
authors.forename: Cesar
authors.initials: C
authors.lastname: Rodriguez-Emmenegger
mesh_terms: D000818:Animals; D057219:Biofouling / Q000517:prevention & control*; D002448:Cell Adhesion; D002478:Cells, Cultured; D008422:Materials Testing; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters; D011485:Protein Binding; D023822:Tissue Engineering
chemical_list: D011091:Polyesters; C016240:polycaprolactone
keywords: antifouling; cell adhesion; nanofibers; polymer brushes; surface-initiated atom transfer radical polymerization (SI-ATRP)