title: Rice α-globulin decreases serum cholesterol concentrations in rats fed a hypercholesterolemic diet and ameliorates atherosclerotic lesions in apolipoprotein E-deficient mice.
abstract: The hypocholesterolemic and antiatherogenic effects of rice α-globulin remain unclear. We investigated the hypocholesterolemic effect of rice α-globulin in rats fed a hypercholesterolemic diet. The rats were divided into 4 groups and were orally administrated the following three proteins or a vehicle for 4weeks: rice protein, rice α-globulin, or soy β-conglycinin at a dose of 100mg/kg body weight or carboxymethylcellulose to the control rats. In the rice α-globulin group, serum cholesterol concentrations were 28% lower than the control group and fecal neutral steroid excretion was increased by 30%. The hypocholesterolemic effect of rice α-globulin was equal to soy β-conglycinin in SD rats fed the hypercholesterolemic diet. However, the serum cholesterol concentrations in the rice protein group did not change compared to the control group. To investigate the antiatherogenic effects of rice α-globulin, male apolipoprotein E-deficient mice were orally administered the same dose of rice α-globulin for 9weeks. The en face lesion area in the aorta was 46% lower than in the control group. In conclusion, administration of rice α-globulin improves hypercholesterolemia in rats fed a hypercholesterolemic diet by increasing the fecal excretion of neutral sterols, and inhibits atherosclerosis development in apolipoprotein E-deficient mice. The anti-atherosclerotic effect exerts by mechanism(s) other than the regulation of serum MCP-1 and NO concentrations.
authors.affiliation: Laboratory of Nutrition Chemistry, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Li-Tao
authors.initials: LT
authors.lastname: Tong
authors.affiliation: Laboratory of Nutrition Chemistry, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Fujimoto
authors.affiliation: Laboratory of Nutrition Chemistry, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Shimizu
authors.affiliation: Laboratory of Nutrition Chemistry, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Tsukino
authors.affiliation: Laboratory of Nutrition Chemistry, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Taiki
authors.initials: T
authors.lastname: Akasaka
authors.affiliation: Laboratory of Nutrition Chemistry, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kato
authors.affiliation: Laboratory of Nutrition Chemistry, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Wakako
authors.initials: W
authors.lastname: Iwamoto
authors.affiliation: Laboratory of Nutrition Chemistry, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Sawako
authors.initials: S
authors.lastname: Shiratake
authors.affiliation: Laboratory of Nutrition Chemistry, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Imaizumi
authors.affiliation: Laboratory of Nutrition Chemistry, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Electronic address: masaos@agr.kyushu-u.ac.jp.
authors.forename: Masao
authors.initials: M
authors.lastname: Sato
mesh_terms: D000510:Alpha-Globulins / Q000032:analysis / Q000737:chemistry*; D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency*; D050197:Atherosclerosis / Q000209:etiology*; D002784:Cholesterol / Q000097:blood / Q000378:metabolism*; D004032:Diet; D006937:Hypercholesterolemia; D008297:Male; D051379:Mice; D012275:Oryza / Q000009:adverse effects* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000510:Alpha-Globulins; D001057:Apolipoproteins E; D002784:Cholesterol
keywords: Anti-atheroslerosis; Apolipoprotein E-deficient mice; Hypocholesterolemia; Rice α-globulin; Soy β-conglycinin; Sprague-Dawley rats