title: Extracellular polysaccharide from Bordetella species reduces high glucose-induced macrophage apoptosis via regulating interaction between caveolin-1 and TLR4.
abstract: Microphage apoptosis is a critical event in atherosclerotic lesions in patients with diabetes. In the present investigation, high glucose treatment inhibited Akt phosphorylation and activated caspase 3 in primary peritoneal macrophage, leading to cell apoptosis. Hypoxia prolonged macrophage survival in high glucose condition. Extracellular polysaccharide from Bordetella species (EPS) further decreased cell apoptosis in response to high glucose during hypoxia. Under high glucose and hypoxic condition, EPS treatment promoted caveolin-1 phosphorylation by recognizing TLR4. Caveolin-1 phosphorylation elevated membrane Glut1 level to accelerate glucose consumption, which should be the reason for protective effect of EPS on macrophage exposed to high glucose. Further investigation demonstrated that TLR4-dependent caveolin-1 phosphorylation induced by EPS promoted association of caveolin-1 with TLR4, which should be critical for activation of TLR4 signaling pathway.
authors.affiliation: Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Cardiology, Shandong Energy Zibo Mining Group Co., Ltd Central Hospital, Zibo 255120, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Lin
authors.affiliation: Department of Center Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Yanliang
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Obstetrics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China. Electronic address: pengwen506@163.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Peng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D001884:Bordetella / Q000737:chemistry; D051242:Caveolin 1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism; D051272:Glucose Transporter Type 1 / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D051197:Toll-Like Receptor 4 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
chemical_list: D051242:Caveolin 1; D051272:Glucose Transporter Type 1; D011135:Polysaccharides, Bacterial; C495678:Slc2a1 protein, mouse; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D005947:Glucose
keywords: Caveolin-1; Extracellular polysaccharide; High glucose; Hypoxia; Macrophage; TLR4