title: Antioxidant capacity and cytotoxicity of sulfated polysaccharide TLH-3 from Tricholoma lobayense.
abstract: Polysaccharide TLH-3 from the fruit body of Tricholoma lobayense Heim has shown outstanding antioxidant activity. In order to further explore it, TLH-3 was successfully modified to obtain a sulfated derivative (STLH-3). The chemical characteristics of STLH-3 and TLH-3 were determined by high performance liquid chromatography and infrared spectroscopy. Antioxidant activity and cytotoxicity of the samples were investigated in vitro. The antioxidant activities of STLH-3 were significantly improved. In the four indicators of antioxidant activity, the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion scavenging activities of STLH-3 have exceeded that of Vitamin C (Vc). The cytotoxicity against tumor cells including human cervical carcinoma cell line (HeLa) and human breast cancer cell line (MCF-7) of STLH-3 was obviously stronger than that of TLH-3. STLH-3 had no toxic on human embryonic lung fibroblasts (HELF) cells. These results suggested that STLH-3 would be a promising bioactive macromolecule for potential applications in the field of health care and pharmaceutical.
authors.affiliation: School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Lu
authors.affiliation: School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China.
authors.forename: Wenna
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Anhui Huaao Biotechnology Pty. Ltd., Hefei 231131, Anhui, PR China.
authors.forename: Shixiong
authors.initials: S
authors.lastname: Yuan
authors.affiliation: School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhou
authors.affiliation: School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China.
authors.forename: Liming
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China.
authors.forename: Qiuying
authors.initials: Q
authors.lastname: Ding
authors.affiliation: School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China.
authors.forename: Zhengnan
authors.initials: Z
authors.lastname: Cai
authors.affiliation: School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China. Electronic address: chenyan91030@yahoo.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000187:drug effects; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008970:Molecular Weight; D010084:Oxidation-Reduction / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D013431:Sulfates / Q000737:chemistry; D013481:Superoxides / Q000037:antagonists & inhibitors; D055439:Tricholoma / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D062610:Fungal Polysaccharides; D013431:Sulfates; D013481:Superoxides
keywords: Antioxidant; Cytotoxicity; Tricholoma lobayense sulfated polysaccharide