title: Online Tools for TALEN Design.
abstract: Transcription activator-like effector nucleases (TALENs) can be exquisitely specific and highly effective genome editing reagents. Specificity and efficacy depend however on good design for minimal off-targeting and strong binding. Several online tools are accessible to aid in this process. Here, we tabulate those tools, noting their functions and key features.
authors.affiliation: Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, 334 Plant Science, Ithaca, NY, 14853, USA. ajb7@cornell.edu.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Bogdanove
authors.affiliation: Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, 334 Plant Science, Ithaca, NY, 14853, USA.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Booher
mesh_terms: D001665:Binding Sites / Q000235:genetics; D019295:Computational Biology / Q000379:methods*; D004268:DNA-Binding Proteins / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D016678:Genome; D020407:Internet; D012984:Software
chemical_list: D004268:DNA-Binding Proteins
keywords: Genome-editing; Off-target prediction; Protein-DNA interaction; TAL effector nucleases; Web-based tools