title: An Epitope-Specific Respiratory Syncytial Virus Vaccine Based on an Antibody Scaffold.
abstract: Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infections in children. We have generated an epitope-specific RSV vaccine by grafting a neutralizing epitope (F-epitope) in its native conformation into an immunoglobulin scaffold. The resulting antibody fusion exhibited strong binding affinity to Motavizumab, an RSV neutralizing antibody, and effectively induced potent neutralizing antibodies in mice. This work illustrates the potential of the immunoglobulin molecule as a scaffold to present conformationally constrained B-cell epitopes.
authors.affiliation: Department of Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037 (USA).
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Luo
authors.affiliation: California Institute for Biomedical Research (Calibr), 11119 N. Torrey Pines Road, La Jolla, CA 92037 (USA).
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: California Institute for Biomedical Research (Calibr), 11119 N. Torrey Pines Road, La Jolla, CA 92037 (USA).
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: California Institute for Biomedical Research (Calibr), 11119 N. Torrey Pines Road, La Jolla, CA 92037 (USA).
authors.forename: Haiqun
authors.initials: H
authors.lastname: Jia
authors.affiliation: California Institute for Biomedical Research (Calibr), 11119 N. Torrey Pines Road, La Jolla, CA 92037 (USA).
authors.forename: Yuhan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: California Institute for Biomedical Research (Calibr), 11119 N. Torrey Pines Road, La Jolla, CA 92037 (USA).
authors.forename: Dawna
authors.initials: D
authors.lastname: Caballero
authors.affiliation: California Institute for Biomedical Research (Calibr), 11119 N. Torrey Pines Road, La Jolla, CA 92037 (USA).
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Du
authors.affiliation: California Institute for Biomedical Research (Calibr), 11119 N. Torrey Pines Road, La Jolla, CA 92037 (USA).
authors.forename: Rongsheng E
authors.initials: RE
authors.lastname: Wang
authors.affiliation: California Institute for Biomedical Research (Calibr), 11119 N. Torrey Pines Road, La Jolla, CA 92037 (USA).
authors.forename: Danling
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037 (USA). schultz@scripps.edu.
authors.forename: Peter G
authors.initials: PG
authors.lastname: Schultz
authors.affiliation: California Institute for Biomedical Research (Calibr), 11119 N. Torrey Pines Road, La Jolla, CA 92037 (USA). fwang@calibr.org.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D014765:Viral Vaccines
keywords: RSV vaccine; antibodies; conformations; polypeptides; protein engineering