title: Validation of a gas chromatography-electron capture detection of T-2 and HT-2 toxins in Chinese herbal medicines and related products after immunoaffinity column clean-up and pre-column derivatization.
abstract: A sensitive, reproducible and accurate gas chromatography-electron capture detection (GC-ECD) method was developed for simultaneous determination of T-2 and HT-2 toxins in Chinese herbal medicines (CHMs) and related products after immunoaffinity column (IAC) clean-up and pre-column derivatization with N-heptafluoro-butyryl imidazole (HFBI). Then, gas chromatography-spectrometry spectrometer (GC-MS) was applied to confirm the positive results and interfering peaks. The limits of detection (LODs) for T-2 and HT-2 toxins were 1.88 and 0.47ng/g, and the recoveries for different CHMs ranged from 89.2% to 99.1% with relative standard deviation (RSD) <6.0% for T-2 and from 85.9% to 99.0% with RSD <8.8% for HT-2 toxin, respectively. The validated method was successfully applied for the determination of T-2 and HT-2 toxins in 89 Chinese herbal medicines and 10 related products from various sources, where it was found that T-2 and HT-2 toxins were not detected in any of the tested samples. These results were reliable by confirmation using GC-MS. Some unknown peaks were interfering peaks not the target toxins.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Kong
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China; School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China; School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Shen
authors.affiliation: School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ou-Yang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China. Electronic address: yangmeihua15@hotmail.com.
authors.forename: Meihua
authors.initials: M
authors.lastname: Yang
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry*; D054928:Validation Studies as Topic
chemical_list: D004365:Drugs, Chinese Herbal; D013605:T-2 Toxin; C012351:HT-2 toxin
keywords: Chinese herbal medicine; GC-ECD; GC–MS; Method validation; T-2 and HT-2 toxins