title: Chemical labeling of intracellular proteins via affinity conjugation and strain-promoted cycloadditions in live cells.
abstract: A versatile chemical labeling approach was developed, where intracellular proteins were first incorporated with a bioorthogonal group via affinity conjugation, and subsequently labeled via strain-promoted cycloaddition reactions in live cells.
authors.affiliation: Chemical Genomics Centre of the Max Planck Society, Otto-Hahn-Str. 15, 44227 Dortmund, Germany. yaowen.wu@mpi-dortmund.mpg.de and Max-Planck Institute for Molecular Physiology, Otto-Hahn-Str. 11, 44227 Dortmund, Germany.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Chemical Genomics Centre of the Max Planck Society, Otto-Hahn-Str. 15, 44227 Dortmund, Germany. yaowen.wu@mpi-dortmund.mpg.de and Max-Planck Institute for Molecular Physiology, Otto-Hahn-Str. 11, 44227 Dortmund, Germany.
authors.forename: Fu
authors.initials: F
authors.lastname: Li
authors.affiliation: Chemical Genomics Centre of the Max Planck Society, Otto-Hahn-Str. 15, 44227 Dortmund, Germany. yaowen.wu@mpi-dortmund.mpg.de and Max-Planck Institute for Molecular Physiology, Otto-Hahn-Str. 11, 44227 Dortmund, Germany.
authors.forename: Yao-Wen
authors.initials: YW
authors.lastname: Wu
mesh_terms: D061565:Cycloaddition Reaction; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D011506:Proteins / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins