title: Novel diesel-oil-degrading bacteria and fungi from the Ecuadorian Amazon rainforest.
abstract: Isolating new diesel-oil-degrading microorganisms from crude-oil contaminated sites and evaluating their degradation capacities are vitally important in the remediation of oil-polluted environments and crude-oil exploitation. In this research, new hydrocarbon-degrading bacteria and fungi were isolated from the crude-oil contaminated soil of the oil-fields in the Amazon rainforest of north-east Ecuador by using a soil enrichment technique. Degradation analysis was tracked by gas chromatography and a flame ionization detector. Under laboratory conditions, maximum degradability of the total n-alkanes reached up to 77.34 and 62.62 removal ratios after 30 days of incubation for the evaporated diesel oil by fungi (isolate-1) and bacteria (isolate-1), respectively. The 16S/18S rDNA sequence analysis indicated that the microorganisms were most closely (99-100%) related to Bacillus cereus (isolate-1), Bacillus thuringiensis (isolate-2), Geomyces pannorum (isolate-1), and Geomyces sp. (isolate-2). Therefore, these strains enable the degradation of hydrocarbons as the sole carbon source, and these findings will benefit these strains in the remediation of oil-polluted environments and oil exploitation.
authors.affiliation: Faculty of Environmental Engineering, Universidad Estatal Amazónica, Puyo, Ecuador E-mail: mnraju8875@gmail.com.
authors.forename: N R
authors.initials: NR
authors.lastname: Maddela
authors.affiliation: Department of Life Sciences, Universidad de las Fuerzas Armadas, ESPE, Quito, Ecuador.
authors.forename: M
authors.initials: M
authors.lastname: Masabanda
authors.affiliation: Instituto de Biotecnología de las Plantas (IBP), Universidad Central Marta Abreu de Las Villas, Santa Clara, Cuba.
authors.forename: M
authors.initials: M
authors.lastname: Leiva-Mora
mesh_terms: D000473:Alkanes / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D005658:Fungi / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D005742:Gasoline / Q000032:analysis / Q000382:microbiology*; D006838:Hydrocarbons / Q000378:metabolism; D010578:Petroleum / Q000378:metabolism*; D065947:Rainforest; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism
chemical_list: D000473:Alkanes; D005742:Gasoline; D006838:Hydrocarbons; D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants