title: A novel far-red fluorescent xenograft model of ovarian carcinoma for preclinical evaluation of HER2-targeted immunotoxins.
abstract: We have created a novel fluorescent model of a human ovarian carcinoma xenograft overexpressing receptor HER2, a promising molecular target of solid tumors. The model is based on a newly generated SKOV-kat cell line stably expressing far-red fluorescent protein Katushka. Katushka is most suitable for the in vivo imaging due to an optimal combination of high brightness and emission in the "window of tissue transparency". The relevance of the fluorescent model for the in vivo monitoring of tumor growth and response to treatment was demonstrated using a newly created HER2-targeted recombinant immunotoxin based on the 4D5scFv antibody and a fragment of the Pseudomonas exotoxin A.
authors.affiliation: Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603950, Russia.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Zdobnova
authors.affiliation: Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603950, Russia.
authors.forename: Evgeniya
authors.initials: E
authors.lastname: Sokolova
authors.affiliation: Institute of Bioorganic Chemistry of The Russian Academy of Sciences, Moscow 117997, Russia.
authors.forename: Oleg
authors.initials: O
authors.lastname: Stremovskiy
authors.affiliation: Institute of Bioorganic Chemistry of The Russian Academy of Sciences, Moscow 117997, Russia.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Karpenko
authors.affiliation: National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: William
authors.initials: W
authors.lastname: Telford
authors.affiliation: Institute of Applied Physics of The Russian Academy of Sciences, Nizhny Novgorod, 603950 Russia.
authors.forename: Ilya
authors.initials: I
authors.lastname: Turchin
authors.affiliation: Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603950, Russia.
authors.forename: Irina
authors.initials: I
authors.lastname: Balalaeva
authors.affiliation: Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603950, Russia.|Institute of Bioorganic Chemistry of The Russian Academy of Sciences, Moscow 117997, Russia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Deyev
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D000922:Immunotoxins / Q000737:chemistry / Q000494:pharmacology*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000037:antagonists & inhibitors*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays; D000097573:Red Fluorescent Protein
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin; D000922:Immunotoxins; D008164:Luminescent Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D011993:Recombinant Fusion Proteins; D000097573:Red Fluorescent Protein; C508053:ERBB2 protein, human
keywords: HER2; far-red fluorescent protein; immunotoxin; ovarian carcinoma; xenograft tumor model