title: Glutaminase 1 is a potential biomarker for chronic post-surgical pain in the rat dorsal spinal cord using differential proteomics.
abstract: Chronic post-surgical pain (CPSP) is a normal and significant symptom in clinical surgery, such as breast operation, biliary tract operation, cesarean operation, uterectomy and thoracic operation. Severe chronic post-surgical pain could increase post-surgical complications, including myocardial ischemia, respiratory insufficiency, pneumonia and thromboembolism. However, the underlying mechanism is still unknown. Herein, a rat CPSP model was produced via thoracotomy. After surgery, in an initial study, 5 out of 12 rats after surgery showed a significant decrease in mechanical withdrawal threshold and/or increase in the number of acetone-evoked responses, and therefore classified as the CPSP group. The remaining seven animals were classified as non-CPSP. Subsequently, open-chest operation was performed on another 30 rats and divided into CPSP and non-CPSP groups after 21-day observation. Protein expression levels in the dorsal spinal cord tissue were determined by 12.5 % SDS-PAGE. Finally, differently expressed proteins were identified by LC MS/MS and analyzed by MASCOT software, followed by Gene Ontology cluster analysis using PANTHER software. Compared with the non-CPSP group, 24 proteins were only expressed in the CPSP group and another 23 proteins expressed differentially between CPSP and non-CPSP group. Western blot further confirmed that the expression of glutaminase 1 (GLS1) was significantly higher in the CPSP than in the non-CPSP group. This study provided a new strategy to identify the spinal proteins, which may contribute to the development of chronic pain using differential proteomics, and suggested that GLS1 may serve as a potential biomarker for CPSP.
authors.affiliation: Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou, China. wanght1981@gmail.com.
authors.forename: Haitang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medicine Science, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Yehua
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Anesthesiology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medicine Science, Beijing, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Anatomy, Histology and Embryology, Peking Union Medical College, School of Basic Medicine, Chinese Academy of Medical Sciences, Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Anesthesiology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medicine Science, Beijing, China.
authors.forename: Ailun
authors.initials: A
authors.lastname: Luo
authors.affiliation: Department of Anesthesiology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medicine Science, Beijing, China. garybeijing@163.com.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D059350:Chronic Pain / Q000175:diagnosis / Q000473:pathology*; D003080:Cold Temperature; D004591:Electrophoresis, Polyacrylamide Gel; D005972:Glutaminase / Q000378:metabolism*; D006930:Hyperalgesia / Q000473:pathology*; D008297:Male; D023421:Models, Animal; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000473:pathology*; D053719:Tandem Mass Spectrometry; D013908:Thoracotomy / Q000009:adverse effects* / Q000379:methods
chemical_list: D015415:Biomarkers; D005972:Glutaminase
keywords: Chronic post-surgical pain; Differential protein expression; Glutaminase 1; LC MS/MS; Proteomics