title: Assembly of Customized TAL Effectors Through Advanced ULtiMATE System.
abstract: Transcription activator-like effectors (TALEs) have been widely applied in gene targeting. Here we describe an advanced ULtiMATE (USER-based Ligation-Mediated Assembly of TAL Effector) system that utilizes USER fusion technique and archive of 512 tetramer templates to achieve highly efficient construction of TALEs, which takes only half a day to accomplish the assembly of any given TALE construct. This system is also suitable for large-scale assembly of TALENs and any other TALE-based constructions.
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Junjiao
authors.initials: J
authors.lastname: Yang
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Shengjie
authors.initials: S
authors.lastname: Guo
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Yuan
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China. wswei@pku.edu.cn.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Wei
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics*; D014974:Xanthomonas / Q000737:chemistry / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D015534:Trans-Activators
keywords: Assembly; TAL effectors; TALENs; ULtiMATE system; USER