title: The Mosquito Repellent Citronellal Directly Potentiates Drosophila TRPA1, Facilitating Feeding Suppression.
abstract: Citronellal, a well-known plant-derived mosquito repellent, was previously reported to repel Drosophila melanogaster via olfactory pathways involving but not directly activating Transient Receptor Potential Ankyrin 1 (TRPA1). Here, we show that citronellal is a direct agonist for Drosophila and human TRPA1s (dTRPA1 and hTRPA1) as well as Anopheles gambiae TRPA1 (agTRPA1). Citronellal-induced activity is isoform-dependent for Drosophila and Anopheles gambiae TRPA1s. The recently identified dTRPA1(A) and ag-TRPA1(A) isoforms showed citronellal-provoked currents with EC50s of 1.0 B1 0.2 and 0.1 B1 0.03 mM, respectively, in Xenopus oocytes, while the sensitivities of TRPA1(B)s were much inferior to those of TRPA1(A)s. Citronellal dramatically enhanced the feeding-inhibitory effect of the TRPA1 agonist N-methylmaleimide (NMM) in Drosophila at an NMM concentration that barely repels flies. Thus, citronellal can promote feeding deterrence of fruit flies through direct action on gustatory dTRPA1, revealing the first isoform-specific function for TRPA1(A).
authors.affiliation: Samsung Biomedical Research Institute, School of Medicine, Sungkyunkwan University, Suwon 16419, Korea.|Department of Anatomy and Cell Biology, School of Medicine, Sungkyunkwan University, Suwon 16419, Korea.
authors.forename: Eun Jo
authors.initials: EJ
authors.lastname: Du
authors.affiliation: Samsung Biomedical Research Institute, School of Medicine, Sungkyunkwan University, Suwon 16419, Korea.|Department of Anatomy and Cell Biology, School of Medicine, Sungkyunkwan University, Suwon 16419, Korea.
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Ahn
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 16419, Korea.
authors.forename: Min Sung
authors.initials: MS
authors.lastname: Choi
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, University of Ulsan, Ulsan 680-749, Korea.|Department of Anatomy and Cell Biology, School of Medicine, Sungkyunkwan University, Suwon 16419, Korea.
authors.forename: Ilmin
authors.initials: I
authors.lastname: Kwon
authors.affiliation: College of Life Sciences, Sejong University, Seoul 05006, Korea.
authors.forename: Hyung-Wook
authors.initials: HW
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 16419, Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Kwon
authors.affiliation: Samsung Biomedical Research Institute, School of Medicine, Sungkyunkwan University, Suwon 16419, Korea.|Department of Anatomy and Cell Biology, School of Medicine, Sungkyunkwan University, Suwon 16419, Korea.
authors.forename: KyeongJin
authors.initials: K
authors.lastname: Kang
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000080462:Acyclic Monoterpenes; D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D000852:Anopheles / Q000187:drug effects; D015220:Calcium Channels; D029721:Drosophila Proteins / Q000819:agonists*; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D005247:Feeding Behavior / Q000187:drug effects*; D006801:Humans; D007302:Insect Repellents / Q000494:pharmacology*; D007473:Ion Channels; D008301:Maleimides / Q000494:pharmacology; D039821:Monoterpenes / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000819:agonists*; D009865:Oocytes; D020033:Protein Isoforms / Q000819:agonists; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels / Q000819:agonists*; D050051:Transient Receptor Potential Channels / Q000819:agonists*; D014982:Xenopus laevis
chemical_list: D000080462:Acyclic Monoterpenes; D000447:Aldehydes; D015220:Calcium Channels; D029721:Drosophila Proteins; D007302:Insect Repellents; D007473:Ion Channels; D008301:Maleimides; D039821:Monoterpenes; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D000074025:TRPA1 Cation Channel; C473350:TRPA1 protein, human; D050052:TRPC Cation Channels; D050051:Transient Receptor Potential Channels; C475684:TrpA1 protein, Drosophila; C527323:N-methylmaleimide; C108217:citronellal
keywords: Anopheles gambiae; Drosophila melanogaster; TRPA1; citronellal; human TRPA1; insect repellent