title: Differential partitioning of antioxidants, including hydroxytyrosol, in human plasma and LDL: implications for their antioxidant activity in vivo.
abstract: In vivo studies of LDL oxidation following consumption of natural phenolic compounds have yielded mixed results. It is reported that the amphiphilic hydroxytyrosol, after addition to human plasma, does not accumulate in LDL but protects plasma lipids, which are extracted together with hydroxytyrosol, from chemically-induced oxidation. Thus, a novel methodology was proposed, which does not rely on LDL separation and subsequent oxidation but is based on the oxidation of total lipids - simultaneously extracted from plasma with antioxidants - to evaluate the effects of micronutrients that do not partition into LDL, after in vivo supplementation.
authors.affiliation: Laboratory of Functional Foods, IMDEA-Food, Madrid, Spain. Electronic address: francesco.visioli@imdea.org.
authors.forename: Francesco
authors.initials: F
authors.lastname: Visioli
authors.affiliation: Department of Pharmacological Sciences, Milan, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Colombo
authors.affiliation: Department of Pharmacological Sciences, Milan, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Galli
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D019587:Dietary Supplements; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000737:chemistry*; D000069463:Olive Oil; D010084:Oxidation-Reduction; D010636:Phenols / Q000378:metabolism*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D008077:Lipoproteins, LDL; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; C099252:oxidized low density lipoprotein; C005975:3,4-dihydroxyphenylethanol; D010626:Phenylethyl Alcohol
keywords: Antioxidants; Hydroxytyrosol; Olive oil; Oxidised LDL; Polyphenols