title: mTOR Activation by PI3K/Akt and ERK Signaling in Short ELF-EMF Exposed Human Keratinocytes.
abstract: Several reports suggest that ELF-EMF exposures interact with biological processes including promotion of cell proliferation. However, the molecular mechanisms by which ELF-EMF controls cell growth are not completely understood. The present study aimed to investigate the effect of ELF-EMF on keratinocytes proliferation and molecular mechanisms involved. Effect of ELF-EMF (50 Hz, 1 mT) on HaCaT cell cycle and cells growth and viability was monitored by FACS analysis and BrdU assay. Gene expression profile by microarray and qRT-PCR validation was performed in HaCaT cells exposed or not to ELF-EMF. mTOR, Akt and MAPKs expressions were evaluated by Western blot analysis. In HaCaT cells, short ELF-EMF exposure modulates distinct patterns of gene expression involved in cell proliferation and in the cell cycle. mTOR activation resulted the main molecular target of ELF-EMF on HaCaT cells. Our data showed the increase of the canonical pathway of mTOR regulation (PI3K/Akt) and activation of ERK signaling pathways. Our results indicate that ELF-EMF selectively modulated the expression of multiple genes related to pivotal biological processes and functions that play a key role in physio-pathological mechanisms such as wound healing.
authors.affiliation: Department of Medicine and Aging Science, University "G. d'Annunzio", Chieti, Italy.
authors.forename: Antonia
authors.initials: A
authors.lastname: Patruno
authors.affiliation: Department of Psychological, Health and Territorial Sciences, University "G. D'Annunzio", Chieti, Italy.
authors.forename: Mirko
authors.initials: M
authors.lastname: Pesce
authors.affiliation: Department of Psychological, Health and Territorial Sciences, University "G. D'Annunzio", Chieti, Italy.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Grilli
authors.affiliation: Department of Medicine and Aging Science, University "G. d'Annunzio", Chieti, Italy.
authors.forename: Lorenza
authors.initials: L
authors.lastname: Speranza
authors.affiliation: Department of Medicine and Aging Science, University "G. d'Annunzio", Chieti, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Franceschelli
authors.affiliation: Department of Medicine and Aging Science, University "G. d'Annunzio", Chieti, Italy.
authors.forename: Maria Anna
authors.initials: MA
authors.lastname: De Lutiis
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University "G. D'Annunzio", Chieti, Italy.
authors.forename: Giovina
authors.initials: G
authors.lastname: Vianale
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University "G. D'Annunzio", Chieti, Italy.
authors.forename: Erica
authors.initials: E
authors.lastname: Costantini
authors.affiliation: Department of Medicine and Aging Science, University "G. d'Annunzio", Chieti, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Amerio
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University "G. D'Annunzio", Chieti, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Muraro
authors.affiliation: Department of Medicine and Aging Science, University "G. d'Annunzio", Chieti, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Felaco
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University "G. D'Annunzio", Chieti, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Reale
mesh_terms: D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000235:genetics; D004574:Electromagnetic Fields; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D005810:Multigene Family / Q000235:genetics; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D058570:TOR Serine-Threonine Kinases / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics; D014945:Wound Healing / Q000235:genetics
chemical_list: D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; C546842:MTOR protein, human