title: Stratified Scaffolds for Osteochondral Tissue Engineering.
abstract: Stratified scaffolds are promising devices finding application in the field of osteochondral tissue engineering. In this scaffold type, different biomaterials are chosen to fulfill specific features required to mimic the complex osteochondral tissue interface, including cartilage, interlayer tissue, and subchondral bone. Here, the biomaterials and fabrication methods currently used to manufacture stratified multilayered scaffolds as well as cell seeding techniques for their characterization are presented.
authors.affiliation: Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstraße 6, 91052, Erlangen, Germany.
authors.forename: Patcharakamon
authors.initials: P
authors.lastname: Nooeaid
authors.affiliation: Institute of Anatomy, Paracelsus Medical University, Salzburg and Nuremberg, Nuremberg, Germany.|Department for Orthopedic, Trauma and Reconstructive Surgery, Charité-University of Medicine, Berlin, Campus Benjamin Franklin, Garystrasse 5, 90419, Berlin, Nuremberg, Germany.
authors.forename: Gundula
authors.initials: G
authors.lastname: Schulze-Tanzil
authors.affiliation: Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstraße 6, 91052, Erlangen, Germany. aldo.boccaccini@ww.uni-erlangen.de.
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
mesh_terms: D000464:Alginates / Q000737:chemistry; D015415:Biomarkers / Q000378:metabolism; D016025:Bone Transplantation; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D020219:Chondrogenesis; D005270:Femur Head / Q000166:cytology* / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D010012:Osteogenesis; D010641:Phenotype; D012038:Regeneration; D044968:Regenerative Medicine / Q000379:methods*; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D000464:Alginates; D015415:Biomarkers; D006603:Hexuronic Acids; D020723:Glucuronic Acid
keywords: Alginate; Biomaterial; Mesenchymal stem cells; Osteochondral tissue engineering; Stratified scaffold