title: ABCG2/BCRP interaction with the sea grass Thalassia testudinum.
abstract: BACKGROUND
  The aqueous ethanolic extract from leaves of the marine plant Thalassia testudinum has shown antioxidant, cytoprotective, and neuroprotective properties. The chemical composition of this extract, rich in polyphenols, could interfere with active transport of drugs out of the cell and circumvent the phenomenon of multidrug resistance (MDR). The extract can act as an MDR modulator through its interaction with efflux transporters. The ABCG2/BCRP has been shown to confer MDR acting in tumor cells.
  
  
  METHODS
  To evaluate the interaction of ABCG2/BCRP with the extract, studies in cells overexpressing human BCRP transporter and its murine ortholog Bcrp1 were performed.
  
  
  CONCLUSIONS
  T. testudinum extract could be included as MDR modulator, as interaction with ABCG2/BCRP has been shown through flow cytometry and MTT assays. The cells overexpressing ABCG2/BCRP in the presence of the extract (25-150 μg/mL) decreased the survival rates of the anti-tumoral mitoxantrone. Our results support its inclusion as a possible MDR modulator against tumor cells that overexpress ABCG2/BCRP.
authors.forename: Verónica
authors.initials: V
authors.lastname: Miguel
authors.forename: Jon A
authors.initials: JA
authors.lastname: Otero
authors.forename: Borja
authors.initials: B
authors.lastname: Barrera
authors.forename: Idania
authors.initials: I
authors.lastname: Rodeiro
authors.forename: Julio G
authors.initials: JG
authors.lastname: Prieto
authors.forename: Gracia
authors.initials: G
authors.lastname: Merino
authors.forename: Ana I
authors.initials: AI
authors.lastname: Álvarez
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002478:Cells, Cultured; D054659:Diketopiperazines / Q000494:pharmacology; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D041743:Herb-Drug Interactions / Q000502:physiology*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D006801:Humans; D031321:Hydrocharitaceae; D007928:Lethal Dose 50; D051379:Mice; D008942:Mitoxantrone / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism* / Q000494:pharmacology; D018515:Plant Leaves
chemical_list: C541506:3-(6-isobutyl-9-methoxy-1,4-dioxo-1,2,3,4,6,7,12,12a-octahydropyrazino(1',2'-1,6)pyrido(3,4-b)indol-3-yl)propionic acid tert-butyl ester; C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; C501412:Abcg2 protein, mouse; D000970:Antineoplastic Agents; D054659:Diketopiperazines; D006576:Heterocyclic Compounds, 4 or More Rings; D009363:Neoplasm Proteins; D010936:Plant Extracts; D008942:Mitoxantrone