title: Comparison of the Microarray-Based Assay, the Real-Time PCR Assay, and the Bidirectional Sequencing Method for CYP2C19 Genotyping.
abstract: BACKGROUND
  Cytochrome P450 2C19 (CYP2C19) is a clinically important enzyme that metabolizes a wide variety of drugs. Recently, some new genetic assays designed to identify the CYP2C19 genotype were introduced.
  
  
  METHODS
  We compared the abilities of the microarray-based Verigene CYP2C19 Test (Nanosphere, USA) and the AccuPower Real-time PCR CYP (*2,*3,*17) assay (Bioneer, Korea) with the ability of the bidirectional sequencing method for the detection of CYP2C19 genotypes in 78 whole blood samples.
  
  
  RESULTS
  Among 78 specimens, 28 were *1/*1, 1 was *1/*17, 27 were *1/*2, 10 were *1/*3, 8 were *2/*2, 1 was *3/*3, and 3 were *2/*3. In the initial test, five samples (6.4%) in the Verigene assay and two samples (2.4%) in the AccuPower assay failed to generate definite results. All retests with both assays generated definitive results. In both assays, the results of CYP2C19 genotyping showed 100% final concordance with those obtained by the direct sequencing method.
  
  
  CONCLUSIONS
  These two assays could be useful for the identification of CYP2C19 genotypes in clinical laboratories.
authors.forename: Hyun-Ki
authors.initials: HK
authors.lastname: Kim
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Kang
authors.forename: Dae-Hyun
authors.initials: DH
authors.lastname: Ko
authors.forename: Tae-Dong
authors.initials: TD
authors.lastname: Jeong
authors.forename: Woochang
authors.initials: W
authors.lastname: Lee
authors.forename: Sail
authors.initials: S
authors.lastname: Chun
authors.forename: Won-Ki
authors.initials: WK
authors.lastname: Min
mesh_terms: D065731:Cytochrome P-450 CYP2C19 / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: C045793:CYP2C19 protein, human; D065731:Cytochrome P-450 CYP2C19