title: Endothelial glycocalyx integrity is preserved in young, healthy men during a single bout of strenuous physical exercise.
abstract: In the present study we aimed to evaluate whether oxidative stress and inflammation induced by strenuous exercise affect glycocalyx integrity and endothelial function. Twenty one young, untrained healthy men performed a maximal incremental cycling exercise - until exhaustion. Markers of glycocalyx shedding (syndecan-1, heparan sulfate and hyaluronic acid), endothelial status (nitric oxide and prostacyclin metabolites - nitrate, nitrite, 6-keto-prostaglandin F(1alpha)), oxidative stress (8-oxo-2'-deoxyguanosine) and antioxidant capacity (uric acid, non-enzymatic antioxidant capacity) as well as markers of inflammation (sVCAM-1 and sICAM-1) were analyzed in venous blood samples taken at rest and at the end of exercise. The applied strenuous exercise caused a 5-fold increase in plasma lactate and hypoxanthine concentrations (p<0.001), a fall in plasma uric acid concentration and non-enzymatic antioxidant capacity (p<10(-4)), accompanied by an increase (p=0.003) in sVCAM-1 concentration. Plasma 6-keto-prostaglandin F(1alpha) concentration increased (p=0.006) at exhaustion, while nitrate and nitrite concentrations were not affected. Surprisingly, no significant changes in serum syndecan-1 and heparan sulfate concentrations were observed. We have concluded, that a single bout of severe-intensity exercise is well accommodated by endothelium in young, healthy men as it neither results in evident glycocalyx disruption nor in the impairment of nitric oxide and prostacyclin production.
authors.affiliation: Department of Muscle Physiology, Chair of Physiology and Biochemistry, Faculty of Rehabilitation, University School of Physical Education, Kraków, Poland. jerzy.zoladz@awf.krakow.pl.
authors.forename: J
authors.initials: J
authors.lastname: Majerczak
authors.forename: K
authors.initials: K
authors.lastname: Duda
authors.forename: S
authors.initials: S
authors.lastname: Chlopicki
authors.forename: G
authors.initials: G
authors.lastname: Bartosz
authors.forename: A
authors.initials: A
authors.lastname: Zakrzewska
authors.forename: A
authors.initials: A
authors.lastname: Balcerczyk
authors.forename: R T
authors.initials: RT
authors.lastname: Smoleński
authors.forename: J A
authors.initials: JA
authors.lastname: Zoladz
mesh_terms: D015415:Biomarkers / Q000097:blood; D004730:Endothelium, Vascular / Q000378:metabolism*; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test / Q000379:methods; D019276:Glycocalyx / Q000378:metabolism*; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D008297:Male; D005082:Physical Exertion / Q000502:physiology*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators