title: Measurement of platelet aggregation functions using whole blood migration ratio in a microfluidic chip.
abstract: Platelets play a major role in maintaining endothelial integrity and hemostasis. Of the various soluble agonists, ADP is an important in vivo stimulus for inducing platelet aggregation. In this study, a simple, rapid, and affordable method was designed for testing bleeding time (BT) and platelet aggregation with a two-channel microfluidic chip. Whole blood migration ratio (MR) from a microchip system was evaluated in comparison to the closure time (CT) from PFA-100 assays (Siemens, Germany) and CD62P expression on platelets. To induce platelet aggregation, a combination of collagen (1.84 mg/ml) and ADP (37.5 mg/ml) were used as agonists. After adding the agonists to samples, whole blood MR from the microchip system was measured. The outcome of the assessment depended on reaction time and agonist concentration. MR of whole blood from the microchip system was significantly correlated with CT from PFA-100 (r = 0.61, p <  0.05, n = 60). In addition, MR was negatively correlated with CD62P expression (r =-0.95, p <  0.05, n = 60). These results suggest that the measurement of MR using agonists is an easy, simple and efficient method for monitoring platelet aggregation in normal and ADP-receptors defective samples, along with the BT test. Thus, usage of the current microfluidic method could expand to diverse applications, including efficacy assessments in platelet therapy.
authors.affiliation: KU-KIST Graduate School of Converging Science and Technology, Seoul, Korea.
authors.forename: Hong Seog
authors.initials: HS
authors.lastname: Seo
authors.affiliation: Department of Emergency Medicine, College of Medicine, Korea University, Guro Gu, Seoul, Korea.
authors.forename: Sung Hyuk
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Korea University, Guro Gu, Seoul, Korea.
authors.forename: Miran
authors.initials: M
authors.lastname: Han
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Korea University, Guro Gu, Seoul, Korea.
authors.forename: Kyeong Ah
authors.initials: KA
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Korea University, Guro Gu, Seoul, Korea.
authors.forename: Chi Hyun
authors.initials: CH
authors.lastname: Cho
authors.affiliation: College of Bionano Technology, Gachon Bionano Research Institute, Gachon University, Seongnam-si, Gyeonggi Do, Korea.
authors.forename: Seong Soo A
authors.initials: SS
authors.lastname: An
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Korea University, Guro Gu, Seoul, Korea.
authors.forename: Chae Seung
authors.initials: CS
authors.lastname: Lim
authors.affiliation: School of Mechanical Engineering, Korea University, Seoul, Korea.
authors.forename: Sehyun
authors.initials: S
authors.lastname: Shin
mesh_terms: D000244:Adenosine Diphosphate / Q000737:chemistry; D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects*; D003094:Collagen / Q000737:chemistry; D005260:Female; D005434:Flow Cytometry; D006487:Hemostasis; D006801:Humans; D008297:Male; D044085:Microfluidics; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood; D010974:Platelet Aggregation; D010976:Platelet Count; D010979:Platelet Function Tests; D013696:Temperature
chemical_list: D019007:P-Selectin; C546937:SELP protein, human; D000244:Adenosine Diphosphate; D003094:Collagen
keywords: ADP; Collagen; PFA-100; Platelet