title: Circulating microparticle signature in coronary and peripheral blood of ST elevation myocardial infarction patients in relation to pain-to-PCI elapsed time.
abstract: BACKGROUND
  Circulating microparticle (cMP) levels are increased in the acute phase of ST-elevation myocardial infarction (STEMI) and associate with microvascular obstruction; however, the precise cMP-parental cell signature and activation level are not elucidated. Here, we aimed to study the cMP signature in STEMI-patients and whether cMP phenotype changes in relation to onset of pain-to-PCI [ischemic time (IT)]-elapsed time.
  
  
  METHODS
  Blood was taken at PCI from the culprit coronary and the peripheral circulation in STEMI-patients (N=40). Two control groups were included: peripheral blood of age-matched patients recovering from STEMI [after 72 h] and of control individuals (N=20/group). cMP-parental origin and activation level were characterized by triple-labeling flow cytometry.
  
  
  RESULTS
  Procoagulant annexin V-positive cMPs bearing parental cell markers as well as markers of activated cells displayed a significantly different profile in STEMI-patients, in control individuals and in patients recovering from STEMI. cMPs derived from monocytes, endothelium, and activated vascular cells were higher in the culprit coronary artery than in peripheral blood in STEMI-patients, especially in patients intervened at short IT. Indeed, cMP levels in coronary blood were inversely related to IT duration (more abundant in thrombi with pain-to-PCI time<180 min).
  
  
  CONCLUSIONS
  A characteristic [CD66b+/CD62E+/CD142+] cMP signature in the systemic circulation reflects the formation of coronary thrombotic occlusions in STEMI-patients. Changes in the cMP signature in the culprit coronary artery blood reveal the sensitivity of MPs to detect the ischemia-elapsed time. Interestingly, cMPs in peripheral blood may be sensitive markers of the thrombo-occlusive vascular process developing in the coronary arteries of STEMI-patients.
authors.affiliation: Cardiovascular Research Center, CSIC-ICCC, IIB-Sant Pau, Barcelona, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Suades
authors.affiliation: Cardiovascular Research Center, CSIC-ICCC, IIB-Sant Pau, Barcelona, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Padró
authors.affiliation: Cardiovascular Research Center, CSIC-ICCC, IIB-Sant Pau, Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Crespo
authors.affiliation: Cardiovascular Research Center, CSIC-ICCC, IIB-Sant Pau, Barcelona, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Ramaiola
authors.affiliation: Department of Interventional Cardiology, Hospital Clinic, Barcelona, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Martin-Yuste
authors.affiliation: Department of Interventional Cardiology, Hospital Clinic, Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Sabaté
authors.affiliation: Acute and Intensive Cardiac Care Unit, Cardiology Department, Hospital Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Sans-Roselló
authors.affiliation: Acute and Intensive Cardiac Care Unit, Cardiology Department, Hospital Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Sionis
authors.affiliation: Cardiovascular Research Center, CSIC-ICCC, IIB-Sant Pau, Barcelona, Spain; Cardiovascular Research Chair, UAB, Barcelona, Spain. Electronic address: lbadimon@csic-iccc.org.
authors.forename: L
authors.initials: L
authors.lastname: Badimon
mesh_terms: D000368:Aged; D015906:Angioplasty, Balloon, Coronary / Q000379:methods; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D054059:Coronary Occlusion / Q000097:blood* / Q000378:metabolism; D003331:Coronary Vessels / Q000378:metabolism; D004562:Electrocardiography; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000378:metabolism / Q000601:surgery; D009204:Myocardial Revascularization / Q000379:methods; D010146:Pain / Q000175:diagnosis / Q000378:metabolism* / Q000601:surgery; D062645:Percutaneous Coronary Intervention / Q000379:methods; D010641:Phenotype; D011237:Predictive Value of Tests; D012720:Severity of Illness Index; D017131:Thrombectomy / Q000379:methods; D013927:Thrombosis / Q000378:metabolism
chemical_list: D015415:Biomarkers
keywords: Biomarkers; Circulating cell-derived microparticles; Myocardial infarction; Myocardial revascularization; Thrombosis