title: Application of Celluclast 1.5L in apple pectin extraction.
abstract: Pectins were extracted from apple pomace with Celluclast 1.5L at a dose of 25, 50 and 75 μl per 1g of material. In obtained pectin, the galacturonic acid (GalA) content, the neutral sugars (NS) profile, the degree of methylation (DM) and acetylation (DAc), the molecular mass, protein, ash and polyphenol levels as well as antioxidant and antitumor activity were determined. The lowest dose of enzymatic preparation resulted in the yield of pectin isolation comparable with acidic treatment (15.3%). Application of higher dose caused further, almost 4% increase in polymer recovery. Enzymatically isolated pectin was characterised by larger molecular mass and contained more GalA of higher DM and DAc than polymer extracted with acid. It was also richer in protein and polyphenols, and had different NS profile, which resulted in higher antiradical activity as well as the ability to inhibit the proliferation and invasion of Caco-2 adenocarcinoma cells.
authors.affiliation: Department of Food Biotechnology, Faculty of Food Technology, Agricultural University of Cracow, 31-149 Kraków, ul. Balicka 122, Poland. Electronic address: a.wikiera@ur.krakow.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wikiera
authors.affiliation: Department of Food Biotechnology, Faculty of Food Technology, Agricultural University of Cracow, 31-149 Kraków, ul. Balicka 122, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Mika
authors.affiliation: Department of Food Biotechnology, Faculty of Food Technology, Agricultural University of Cracow, 31-149 Kraków, ul. Balicka 122, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Starzyńska-Janiszewska
authors.affiliation: Department of Food Biotechnology, Faculty of Food Technology, Agricultural University of Cracow, 31-149 Kraków, ul. Balicka 122, Poland.
authors.forename: Bożena
authors.initials: B
authors.lastname: Stodolak
mesh_terms: D000107:Acetylation; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D055162:Biocatalysis; D018938:Caco-2 Cells; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005591:Chemical Fractionation / Q000379:methods; D006603:Hexuronic Acids / Q000032:analysis; D006801:Humans; D027845:Malus / Q000737:chemistry*; D008745:Methylation; D009369:Neoplasms / Q000188:drug therapy; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D006603:Hexuronic Acids; C007819:galacturonic acid; D010368:Pectins
keywords: Bioactivity; Celluclast; Composition; Enzyme; Extraction; Pectin