title: Library Construction for Mutation Identification by Whole-Genome Sequencing.
abstract: Next-generation sequencing provides a rapid and powerful method for mutation identification. Herein is described a workflow for sample preparation to allow the simultaneous mapping and identification of candidate mutations by whole-genome sequencing in Caenorhabditis elegans. The protocol is designed for small numbers of worms to accommodate classes of mutations, such as lethal and sterile alleles, that are difficult to identify by traditional means.
authors.affiliation: National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892, USA. smithhe2@niddk.nih.gov.
authors.forename: Harold E
authors.initials: HE
authors.lastname: Smith
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics; D004252:DNA Mutational Analysis; D015723:Gene Library; D016678:Genome; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D009154:Mutation
keywords: NGS library construction; Polymorphism mapping; Sequence variant detection