title: Expression and immunogenic characterization of recombinant gp350 for developing a subunit vaccine against Epstein-Barr virus.
abstract: Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus that is linked to the development of various malignancies. There is an urgent need for effective vaccines against EBV. EBV envelope glycoprotein gp350 is an attractive candidate for a prophylactic vaccine. This study was undertaken to produce the truncated (codons 1-443) gp350 protein (gp350(1-443)) in Pichia pastoris and evaluate its immunogenicity. The gp350(1-443) protein was expressed as a secretory protein with an N-terminal His-tag in P. pastoris and purified through Ni-NTA chromatography. Immunization with the recombinant gp350(1-443) could elicit high levels of gp350(1-443)-specific antibodies in mice. Moreover, gp350(1-443)-immunized mice developed strong lymphoproliferative and Th1/Th2 cytokine responses. Furthermore, the recombinant gp350(1-443) could stimulate CD4(+) and CD8(+) T cell responses in vaccinated mice. Collectively, these findings demonstrated that the yeast-expressed gp350(1-443) retained strong immunogenicity. This study will provide a useful source for developing EBV subunit vaccine candidates.
authors.affiliation: Institute for Translational Medicine, Medical College of Qingdao University, Qingdao, 266021, China. wangman@qdu.edu.cn.
authors.forename: Man
authors.initials: M
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Han
authors.affiliation: Institute for Translational Medicine, Medical College of Qingdao University, Qingdao, 266021, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Institute for Translational Medicine, Medical College of Qingdao University, Qingdao, 266021, China.
authors.forename: Li'ao
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute for Translational Medicine, Medical College of Qingdao University, Qingdao, 266021, China.
authors.forename: Zhixia
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, China. wangyefu@whu.edu.cn.
authors.forename: Yefu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D020031:Epstein-Barr Virus Infections / Q000276:immunology / Q000821:virology*; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D022223:Vaccines, Subunit; D014759:Viral Envelope Proteins; D014765:Viral Vaccines
keywords: EBV; Immunogenicity; Pichia pastoris; Recombinant gp350; Subunit vaccine