title: In Vitro and In Vivo Comparison of Selected Ga-68 and Zr-89 Labelled Siderophores.
abstract: Some [(68)Ga]siderophores show promise in specific and sensitive imaging of infection. Here, we compare the in vitro and in vivo behaviour of selected Ga-68 and Zr-89 labelled siderophores.
  
  
  
  Radiolabelling was performed in HEPES or sodium acetate buffer systems. Radiochemical purity of labelled siderophores was determined using chromatography. Partition coefficients, in vitro stability and protein binding affinities were determined. Ex vivo biodistribution and animal imaging was studied in mice.
  
  
  
  Certain differences among studied siderophores were observed in labelling efficiency. Protein binding and stability tests showed highest stabilities and lowest protein binding affinities for Ga-68 and [(89)Zr]triacetylfusarinine C (TAFC). All studied Ga-68 and [(89)Zr]siderophores exhibited a similar biodistribution and pharmacokinetics in mice with the exception of [(89)Zr]ferrioxamine E (FOXE).
  
  
  
  Zr-89 and [(68)Ga]siderophores showed analogous in vitro and in vivo behaviour. Tested [(89)Zr]siderophores could be applied for longitudinal positron emission tomography (PET) studies of fungal infections and especially TAFC for the development of novel bioconjugates.
authors.affiliation: Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 5, CZ-77900, Olomouc, Czech Republic. milospetrik@seznam.cz.
authors.forename: Milos
authors.initials: M
authors.lastname: Petrik
authors.affiliation: Clinical Department of Nuclear Medicine, Medical University Innsbruck, Innsbruck, Austria.
authors.forename: Chuangyan
authors.initials: C
authors.lastname: Zhai
authors.affiliation: Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 5, CZ-77900, Olomouc, Czech Republic.
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Novy
authors.affiliation: Laboratory of Growth Regulators, Centre of the Region Hana for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR & Palacky University, Olomouc, Czech Republic.
authors.forename: Lubor
authors.initials: L
authors.lastname: Urbanek
authors.affiliation: Division of Molecular Biology/Biocenter, Medical University Innsbruck, Innsbruck, Austria.
authors.forename: Hubertus
authors.initials: H
authors.lastname: Haas
authors.affiliation: Clinical Department of Nuclear Medicine, Medical University Innsbruck, Innsbruck, Austria.
authors.forename: Clemens
authors.initials: C
authors.lastname: Decristoforo
mesh_terms: D000818:Animals; D005260:Female; D005710:Gallium Radioisotopes / Q000737:chemistry*; D008807:Mice, Inbred BALB C; D049268:Positron-Emission Tomography; D011868:Radioisotopes; D017262:Siderophores / Q000737:chemistry*; D014018:Tissue Distribution; D014057:Tomography, X-Ray Computed; D015040:Zirconium / Q000737:chemistry*
chemical_list: D005710:Gallium Radioisotopes; D011868:Radioisotopes; D017262:Siderophores; D015040:Zirconium
keywords: Gallium-68; Imaging; PET; Siderophores; Zirconium-89