title: Metagenomic Human Repiratory Air in a Hospital Environment.
abstract: Hospital-acquired infection (HAI) or nosocomial infection is an issue that frequent hospital environment. We believe conventional regulated Petri dish method is insufficient to evaluate HAI. To address this problem, metagenomic sequencing was applied to screen airborne microbes in four rooms of Beijing Hospital. With air-in amount of sampler being setup to one person's respiration quantity, metagenomic sequencing identified huge numbers of species in the rooms which had already qualified widely accepted petridish exposing standard, imposing urgency for new technology. Meanwhile,the comparative culture only got small portion of recovered species and remain blind for even cultivable pathogens reminded us the limitations of old technologies. To the best of our knowledge, the method demonstrated in this study could be broadly applied in hospital indoor environment for various monitoring activities as well as HAI study. It is also potential as a transmissible pathogen real-time modelling system worldwide.
authors.affiliation: Genomics and Synthetic Biology Center, Tsinghua University, Beijing, China.
authors.forename: Yi Yu
authors.initials: YY
authors.lastname: Lai
authors.affiliation: Department of Respiratory and Critical Care Medicine, Beijing Hospital, Beijing, China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Li
authors.affiliation: Genomics and Synthetic Biology Center, Tsinghua University, Beijing, China; School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Lang
authors.affiliation: Department of Respiratory and Critical Care Medicine, Beijing Hospital, Beijing, China.
authors.forename: Xunliang
authors.initials: X
authors.lastname: Tong
authors.affiliation: Genomics and Synthetic Biology Center, Tsinghua University, Beijing, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Genomics and Synthetic Biology Center, Tsinghua University, Beijing, China.
authors.forename: Jianhuo
authors.initials: J
authors.lastname: Fang
authors.affiliation: Genomics and Synthetic Biology Center, Tsinghua University, Beijing, China.
authors.forename: Jingli
authors.initials: J
authors.lastname: Xing
authors.affiliation: Department of Hospital Infection Control, Beijing Hospital, Beijing, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Cai
authors.affiliation: Department of Medicine Laboratory, Beijing Hospital, Beijing, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Xu
authors.affiliation: State key laboratory of precision measurement technology and instruments, Tsinghua university, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Beijing Hospital, Beijing Institute of Geriatrics, Key Laboratory of Geriatrics, Ministry of Health, Beijing, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.affiliation: Genomics and Synthetic Biology Center, Tsinghua University, Beijing, China; School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Geng
authors.initials: G
authors.lastname: Tian