title: Volatile Organic Metabolites Identify Patients with Mesangial Proliferative Glomerulonephritis, IgA Nephropathy and Normal Controls.
abstract: Urinary volatile organic compounds (VOCs) analysis for kidney diseases has attracted a large amount of scientific interest recently, and urinary metabolite analysis has already been applied to many diseases. Urine was collected from 15 mesangial proliferative glomerulonephritis (MsPGN) patients, 21 IgA nephropathy (IgAN) patients and 15 healthy controls. Solid phase microextraction-chromatography- mass spectrometry (SPME-GC-MS) was used to analyse the urinary metabolites. The statistical methods principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLSDA) were performed to process the final data. Five metabolites were significantly greater in the group of MsPGN patients than in the normal control group (P < 0.05) while three metabolites were found at increased levels in the group of IgAN patients compared with the normal controls (P < 0.05). In addition, five metabolites were significantly increased in the group of IgAN patients compared with the MsPGN patients (P < 0.05). These five metabolites may be specific biomarkers for distinguishing between MsPGN and IgAN. The analysis of urinary VOCs appears to have potential clinical applications as a diagnostic tool.
authors.affiliation: Department of Anesthesiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Changsong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Nephrology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Mingao
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Pi
authors.affiliation: Department of Anesthesiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Hongshuang
authors.initials: H
authors.lastname: Tong
authors.affiliation: Department of Anesthesiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Nephrology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Anesthesiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Enyou
authors.initials: E
authors.lastname: Li
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D016022:Case-Control Studies; D016002:Discriminant Analysis; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005920:Glomerular Mesangium / Q000378:metabolism / Q000473:pathology*; D005922:Glomerulonephritis, IGA / Q000175:diagnosis* / Q000378:metabolism; D015432:Glomerulonephritis, Membranoproliferative / Q000175:diagnosis* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D025341:Principal Component Analysis; D052617:Solid Phase Microextraction / Q000379:methods; D016482:Urinalysis / Q000379:methods; D055549:Volatile Organic Compounds / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D055549:Volatile Organic Compounds