title: Significantly increased PELP1 protein expression in primary and metastatic triple-negative breast carcinoma: comparison with GATA3 expression and PELP1's potential role in triple-negative breast carcinoma.
abstract: PELP1 is a novel coregulator of nuclear hormone receptors and is implicated in playing a role in driving breast cancer and enhancing metastatic potential. The PELP1 protein expression and potential role of PELP1 in triple-negative breast carcinoma (TNBC) have not been well characterized. We investigated PELP1 expression by immunohistochemistry in primary and metastatic triple-negative tumors in human tissues and compared its expression with GATA-binding protein 3 (GATA3), a novel diagnostic marker for TNBC. We examined the expression of PELP1 and GATA3 in 70 primary TNBC cases and found that PELP1 had a significantly higher frequency of expression compared to GATA3 (96% versus 46%; P < .0001). The mean extent score of expression of PELP1 was also significantly higher than GATA3's expression (3.87 ± 0.07 versus 0.91 ± 0.15; P < .0001). PELP1 had stronger staining intensity than GATA3. Furthermore, PELP1 immunoreactivity was consistently maintained in paired primary and metastatic TNBC cases (100%). The frequency of PELP1 expression (100%) in metastatic triple-negative tumors was higher than that of GATA3 (40%) in the same tumors (P < .0001). These findings indicate that PELP1 is a much more sensitive marker than GATA3 for TNBCs. PELP1 may have diagnostic utility for metastatic TNBC in appropriate settings, such as history of primary TNBC in cases where the primary is negative for GATA3, mammaglobin, and GCDFP-15. The diffuse and strong nuclear immunoreactivity of PELP1 in most cases suggests that PELP1 may be a molecular target for the treatment of TNBC. We hope that this study will provide insights into the role of PELP1 in TNBC.
authors.affiliation: Department of Pathology, UT Southwestern Medical Center, Dallas, TX 75390-9073.
authors.forename: Daniel N
authors.initials: DN
authors.lastname: Dang
authors.affiliation: Department of Urology, UT Southwestern Medical Center, Dallas, TX 75390-9073.
authors.forename: Ganesh
authors.initials: G
authors.lastname: Raj
authors.affiliation: Department of Pathology, UT Southwestern Medical Center, Dallas, TX 75390-9073.
authors.forename: Venetia
authors.initials: V
authors.lastname: Sarode
authors.affiliation: Department of Pathology, UT Southwestern Medical Center, Dallas, TX 75390-9073.
authors.forename: Kyle H
authors.initials: KH
authors.lastname: Molberg
authors.affiliation: Department of Obstetrics & Gynecology, University of Texas Health Science Center, San Antonio, TX 78229.
authors.forename: Ratna K
authors.initials: RK
authors.lastname: Vadlamudi
authors.affiliation: Department of Pathology, UT Southwestern Medical Center, Dallas, TX 75390-9073. Electronic address: yan.peng@utsouthwestern.edu.
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D056970:Co-Repressor Proteins / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D050990:GATA3 Transcription Factor / Q000032:analysis / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000473:pathology; D046888:Tissue Array Analysis; D014157:Transcription Factors / Q000032:analysis / Q000096:biosynthesis*; D064726:Triple Negative Breast Neoplasms / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D056970:Co-Repressor Proteins; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; C438485:PELP1 protein, human; D014157:Transcription Factors
keywords: GATA3; Immunohistochemistry; Metastasis; PELP1; Triple-negative breast carcinoma