title: Microfluidic Synthesis of Hybrid Nanoparticles with Controlled Lipid Layers: Understanding Flexibility-Regulated Cell-Nanoparticle Interaction.
abstract: The functionalized lipid shell of hybrid nanoparticles plays an important role for improving their biocompatibility and in vivo stability. Yet few efforts have been made to critically examine the shell structure of nanoparticles and its effect on cell-particle interaction. Here we develop a microfluidic chip allowing for the synthesis of structurally well-defined lipid-polymer nanoparticles of the same sizes, but covered with either lipid-monolayer-shell (MPs, monolayer nanoparticles) or lipid-bilayer-shell (BPs, bilayer nanoparticles). Atomic force microscope and atomistic simulations reveal that MPs have a lower flexibility than BPs, resulting in a more efficient cellular uptake and thus anticancer effect than BPs do. This flexibility-regulated cell-particle interaction may have important implications for designing drug nanocarriers.
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology , Beijing 100190, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology , Beijing 100190, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Jiuling
authors.initials: J
authors.lastname: Wang
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University , DK-8000 Aarhus C, Denmark.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology , Beijing 100190, China.
authors.forename: Baoquan
authors.initials: B
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University , DK-8000 Aarhus C, Denmark.
authors.forename: Mingdong
authors.initials: M
authors.lastname: Dong
authors.affiliation: National Creative Research Initiative Center for Single-Molecule Systems Biology and Department of Physics, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 305-701, South Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Ryu
authors.affiliation: National Creative Research Initiative Center for Single-Molecule Systems Biology and Department of Physics, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 305-701, South Korea.
authors.forename: Tae-Young
authors.initials: TY
authors.lastname: Yoon
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology , Beijing 100190, China.|State Key Laboratory of Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Shi
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology , Beijing 100190, China.
authors.forename: Jiashu
authors.initials: J
authors.lastname: Sun
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology , Beijing 100190, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use; D002584:Cervix Uteri / Q000187:drug effects / Q000473:pathology; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D006367:HeLa Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D044085:Microfluidics / Q000379:methods*; D056004:Molecular Dynamics Simulation; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008051:Lipid Bilayers; D011108:Polymers
keywords: drug deliver; interfaces; lipids; microfluidics; nanostructures