title: Identification of high risk anaplastic gliomas by a diagnostic and prognostic signature derived from mRNA expression profiling.
abstract: Anaplastic gliomas are characterized by variable clinical and genetic features, but there are few studies focusing on the substratification of anaplastic gliomas. To identify a more objective and applicable classification of anaplastic gliomas, we analyzed whole genome mRNA expression profiling of four independent datasets. Univariate Cox regression, linear risk score formula and receiver operating characteristic (ROC) curve were applied to derive a gene signature with best prognostic performance. The corresponding clinical and molecular information were further analyzed for interpretation of the different prognosis and the independence of the signature. Gene ontology (GO), Gene Set Variation Analysis (GSVA) and Gene Set Enrichment Analysis (GSEA) were performed for functional annotation of the differences. We found a three-gene signature, by applying which, the anaplastic gliomas could be divided into low risk and high risk groups. The two groups showed a high concordance with grade II and grade IV gliomas, respectively. The high risk group was more aggressive and complex. The three-gene signature showed diagnostic and prognostic value in anaplastic gliomas.
authors.affiliation: Department of Molecular Neuropathology, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.|Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.|Center of Brain Tumor, Beijing Institute for Brain Disorders, Beijing, China.|China National Clinical Research Center for Neurological Diseases, Beijing, China.
authors.forename: Chuan-Bao
authors.initials: CB
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Department of Molecular Neuropathology, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.|Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.|Center of Brain Tumor, Beijing Institute for Brain Disorders, Beijing, China.|China National Clinical Research Center for Neurological Diseases, Beijing, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Jin-Quan
authors.initials: JQ
authors.lastname: Cai
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.|Center of Brain Tumor, Beijing Institute for Brain Disorders, Beijing, China.|China National Clinical Research Center for Neurological Diseases, Beijing, China.
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Qing-Bin
authors.initials: QB
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.|China National Clinical Research Center for Neurological Diseases, Beijing, China.
authors.forename: Zhao-Shi
authors.initials: ZS
authors.lastname: Bao
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.|China National Clinical Research Center for Neurological Diseases, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Molecular Neuropathology, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.|Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.|Center of Brain Tumor, Beijing Institute for Brain Disorders, Beijing, China.|China National Clinical Research Center for Neurological Diseases, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D020869:Gene Expression Profiling; D005910:Glioma / Q000175:diagnosis* / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger
keywords: anaplastic glioma; diagnosis; mRNA; prognosis; signature