title: Alteration of human serum albumin tertiary structure induced by glycation. Spectroscopic study.
abstract: The modification of human serum albumin (HSA) structure by non-enzymatic glycation is one of the underlying factors that contribute to the development of complications of diabetes and neurodegenerative diseases. The aim of the present work was to estimate how glycation of HSA altered its tertiary structure. Changes of albumin conformation were investigated by comparison of glycated (gHSA) and non-glycated human serum albumin (HSA) absorption spectra, red edge excitation shift (REES) and synchronous spectra. Effect of glycation on human serum albumin tertiary structure was also investigated by (1)H NMR spectroscopy. Formation of gHSA Advanced Glycation End-products (AGEs) caused absorption of UV-VIS light between 310 nm and 400 nm while for non-glycated HSA in this region no absorbance has been registered. Analysis of red edge excitation shift effect allowed for observation of structural changes of gHSA in the hydrophobic pocket containing the tryptophanyl residue. Moreover changes in the microenvironment of tryptophanyl and tyrosyl residues brought about AGEs on the basis of synchronous fluorescence spectroscopy have been confirmed. The influence of glycation process on serum albumin binding to 5-dimethylaminonaphthalene-1-sulfonamide (DNSA), 2-(p-toluidino) naphthalene-6-sulfonic acid (TNS), has been studied. Fluorescence analysis showed that environment of both binding site I and II is modified by galactose glycation.
authors.affiliation: School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Department of Physical Pharmacy, 41-200 Sosnowiec, Jagiellońska 4, Poland. Electronic address: aszkudlarek@sum.edu.pl.
authors.forename: A
authors.initials: A
authors.lastname: Szkudlarek
authors.affiliation: School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Department of Physical Pharmacy, 41-200 Sosnowiec, Jagiellońska 4, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Maciążek-Jurczyk
authors.affiliation: School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Department of Physical Pharmacy, 41-200 Sosnowiec, Jagiellońska 4, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Chudzik
authors.affiliation: School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Department of Physical Pharmacy, 41-200 Sosnowiec, Jagiellońska 4, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Równicka-Zubik
authors.affiliation: School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Department of Physical Pharmacy, 41-200 Sosnowiec, Jagiellońska 4, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Sułkowska
mesh_terms: D003619:Dansyl Compounds / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D009282:Naphthalenesulfonates / Q000378:metabolism; D017434:Protein Structure, Tertiary; D066244:Proton Magnetic Resonance Spectroscopy; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D003619:Dansyl Compounds; D009282:Naphthalenesulfonates; D012709:Serum Albumin; C001026:5-dimethylaminonaphthalene-1-sulfonamide; C009497:2-(4-toluidino)-6-naphthalenesulfonic acid
keywords: AGEs; Glycation; Human serum albumin; Spectroscopic analysis