title: The role of microRNA-1 and microRNA-206 in the proliferation and differentiation of bovine skeletal muscle satellite cells.
abstract: MicroRNAs (miRNAs) have been found to play essential roles in muscle cell proliferation and differentiation. MicroRNA-1 (miR-1) and microRNA-206 (miR-206), which are similar and have the same seed sequence, have specific roles in modulating skeletal muscle proliferation and differentiation in vitro and in vivo. However, there is no information about their function during bovine skeletal muscle satellite cell development. In this study, the profiles of miR-1 and miR-206 and their biological functions in bovine skeletal muscle cell development was investigated. The target genes were predicted, and we used a dual-luciferase reporter assay to demonstrate that miR-1 and miR-206 directly targeted the 3' untranslated region (3'UTR) of paired-box transcription factor Pax7 and histone deacetylase 4 (HDAC4). We showed that miR-1 and miR-206 facilitate bovine skeletal muscle satellite cell myogenic differentiation by restricting the expression of their target gene and that inhibition of miR-1 and miR-206 increased the Pax7 and HDAC4 protein levels and substantially enhanced satellite cell proliferation. Therefore, our results revealed the mechanism in which miR-1 and miR-206 positively regulate bovine skeletal muscle satellite cell myogenic differentiation via Pax7 and HDAC4 downregulation.
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Dai
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.|College of Veterinary Medicine and Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Yi Min
authors.initials: YM
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors.forename: Wei Ran
authors.initials: WR
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors.forename: Xin Feng
authors.initials: XF
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China. xiangbinding@163.com.
authors.forename: Xiang Bin
authors.initials: XB
authors.lastname: Ding
authors.affiliation: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China. guohong64@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
mesh_terms: D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D051763:PAX7 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism*
chemical_list: C544547:MIRN1 microRNA, human; C546087:MIRN206 microRNA, human; D035683:MicroRNAs; D051763:PAX7 Transcription Factor; D006655:Histone Deacetylases
keywords: Bovine; MicroRNA-1; MicroRNA-206; Myogenic differentiation; Skeletal muscle satellite cells