title: A novel coumarin derivative, 8-methoxy chromen-2-one alleviates collagen induced arthritis by down regulating nitric oxide, NFκB and proinflammatory cytokines.
abstract: Ruta graveolens (Rue) is a well-known medicinal plant having anti-inflammatory and other healing properties. This contains many active phytochemicals such as coumarins which possess anti-inflammatory and anti-cancer activities. The present study was carried out to evaluate the therapeutic potential of a newly isolated coumarin derivative from rue plant, 8-methoxy-chromen-2-one (MCO) in the collagen induced arthritic (CIA) rat model. MCO showed inhibition of cytokines and NF-κB in LPS stimulated J774 cells which prompted its possible use in animal. In CIA, arthritic index and arthritic score reduced markedly within 15days of MCO treatment at doses of 2mg and 20mg per kg body weight. Alleviation of joint damage in CIA animals on treatment with MCO was evident from radiographic and histological data. Behavioral studies by open field tests also showed convalescence in the MCO treated CIA rats. Further, escalated plasma levels of pro-inflammatory cytokines TNF-α, IL-1β and IL-6, and also nitric oxide reduced significantly with the treatment. All these results indicate the therapeutic efficacy of MCO and its possible use as an anti-arthritic drug.
authors.affiliation: Genomics and Molecular Medicine, CSIR - Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India; Product Development Cell-II, DBT-National Institute of Immunology, New Delhi - 110067, India. Electronic address: sahudebasis@nii.ac.in.
authors.forename: Debasis
authors.initials: D
authors.lastname: Sahu
authors.affiliation: Genomics and Molecular Medicine, CSIR - Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India; Immuno-Genomics and Systems Biology, DBT-Institute of Life Sciences, Nalco Nagar Road, Chandrasekharpur, Bhubaneshwar, Odisha, India. Electronic address: sunilraghav@ils.res.in.
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Raghav
authors.affiliation: Genomics and Molecular Medicine, CSIR - Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India. Electronic address: hemlata.gautam@igib.in.
authors.forename: Hemlata
authors.initials: H
authors.lastname: Gautam
authors.affiliation: Genomics and Molecular Medicine, CSIR - Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India. Electronic address: hasidas@yahoo.com.
authors.forename: Hasi R
authors.initials: HR
authors.lastname: Das
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000473:pathology*; D001522:Behavior, Animal / Q000187:drug effects; D002460:Cell Line; D003374:Coumarins / Q000627:therapeutic use*; D016207:Cytokines / Q000037:antagonists & inhibitors*; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D007596:Joints / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D051381:Rats; D017208:Rats, Wistar; D032105:Ruta / Q000737:chemistry
chemical_list: C000610762:8-methoxychromen-2-one; D003374:Coumarins; D016207:Cytokines; D016328:NF-kappa B; D009569:Nitric Oxide
keywords: 8-Methoxy-chromen-2-one; Anti-inflammatory; Collagen induced arthritis; Rheumatoid arthritis; Ruta graveolens