title: Identification of immunodominant proteins from Mannheimia haemolytica and Histophilus somni by an immunoproteomic approach.
abstract: Mannheimia haemolytica and Histophilus somni are frequently isolated from diseased cattle with bovine respiratory disease (BRD). They compromise animal lung function and the immune responses generated are not sufficient to limit infection. Identification of specific immunogenic antigens for vaccine development represents a great challenge. Immunogenic proteins were identified by immunoproteomic approach with sera from cattle immunized with a commercial cellular vaccine of M. haemolytica and H. somni. Proteins of M. haemolytica were identified as solute ABC transporter, iron-binding protein, and hypothetical protein of capsular biosynthesis. Histophilus somni proteins correspond to porin, amino acid ABC transporter, hypothetical outer membrane protein, cysteine synthase, and outer membrane protein P6. Although these antigens share strong similarities with other proteins from animal pathogens, the ABC system proteins have been associated with virulence and these proteins could be considered as potential vaccine candidates for BRD.
authors.affiliation: Medical and Pharmaceutical Biotechnology, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Av. Normalistas 800, Colinas de la Normal, 44270 Guadalajara, Jalisco, México.
authors.forename: Angel H
authors.initials: AH
authors.lastname: Alvarez
authors.affiliation: Medical and Pharmaceutical Biotechnology, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Av. Normalistas 800, Colinas de la Normal, 44270 Guadalajara, Jalisco, México.
authors.forename: Abel
authors.initials: A
authors.lastname: Gutiérrez-Ortega
authors.affiliation: Medical and Pharmaceutical Biotechnology, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Av. Normalistas 800, Colinas de la Normal, 44270 Guadalajara, Jalisco, México.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Hernández-Gutiérrez
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D007162:Immunoproteins / Q000235:genetics / Q000378:metabolism*; D016978:Mannheimia haemolytica; D015661:Pasteurellaceae / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007162:Immunoproteins