title: Conditioned Medium From Mesenchymal Stem Cells Enhances Early Bone Regeneration After Maxillary Sinus Floor Elevation in Rabbits.
abstract: OBJECTIVE
  Conditioned medium from stem cells contains growth factors and has a promising prospect for use in regenerative medicine. In this study, the effects of marrow-derived stem cell-conditioned medium (MSC-CM) on bone regeneration after maxillary sinus floor elevation were examined in rabbits.
  
  
  METHODS
  Rabbit bone marrow-derived stem cells (rMSCs) were cultured with MSC-CM for 48 hours, and their mobilization and proliferation were evaluated. Beta-tricalcium phosphate scaffolds were impregnated with MSC-CM and grafted in the rabbit maxillary sinus cavities. At 2, 4, and 8 weeks after transplantation, sections of the newly formed bone were evaluated histologically and by immunohistochemical staining.
  
  
  RESULTS
  MSC-CM increased the migration and proliferation of rMSCs and the early bone regeneration in rabbit sinus. Cell proliferation and vascularization were increased at 2 weeks after implantation of grafts impregnated with MSC-CM compared to controls, indicating that MSC-CM is effective at the early phase of bone regeneration.
  
  
  CONCLUSIONS
  MSC-CM is a promising novel therapeutic agent to promote bone regeneration after maxillary sinus floor elevation.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Katagiri
authors.forename: Masashi
authors.initials: M
authors.lastname: Osugi
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Kinoshita
authors.forename: Hideharu
authors.initials: H
authors.lastname: Hibi
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D003758:Dental Implantation, Endosseous / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D016348:Osseointegration / Q000187:drug effects / Q000502:physiology; D011817:Rabbits; D059546:Sinus Floor Augmentation / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D017077:Culture Media, Conditioned