title: Engineered Adhesion Peptides for Improved Silicon Adsorption.
abstract: Engineering peptides that present selective recognition and high affinity for a material is a major challenge for assembly-driven elaboration of complex systems with wide applications in the field of biomaterials, hard-tissue regeneration, and functional materials for therapeutics. Peptide-material interactions are of vital importance in natural processes but less exploited for the design of novel systems for practical applications because of our poor understanding of mechanisms underlying these interactions. Here, we present an approach based on the synthesis of several truncated peptides issued from a silicon-specific peptide recovered via phage display technology. We use the photonic response provided by porous silicon microcavities to evaluate the binding efficiency of 14 different peptide derivatives. We identify and engineer a short peptide sequence (SLVSHMQT), revealing the highest affinity for p(+)-Si. The molecular recognition behavior of the obtained peptide fragment can be revealed through mutations allowing identification of the preferential affinity of certain amino acids toward silicon. These results constitute an advance in both the engineering of peptides that reveal recognition properties for silicon and the understanding of biomolecule-material interactions.
authors.affiliation: Laboratoire Charles Coulomb (L2C), UMR 5221 CNRS-Université de Montpellier , Montpellier, France.
authors.forename: Sathish Kumar
authors.initials: SK
authors.lastname: Ramakrishnan
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Université de Montpellier , 15 Avenue Charles Flahault, Montpellier, France.
authors.forename: Said
authors.initials: S
authors.lastname: Jebors
authors.affiliation: Laboratoire Charles Coulomb (L2C), UMR 5221 CNRS-Université de Montpellier , Montpellier, France.
authors.forename: Marta
authors.initials: M
authors.lastname: Martin
authors.affiliation: Laboratoire Charles Coulomb (L2C), UMR 5221 CNRS-Université de Montpellier , Montpellier, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Cloitre
authors.affiliation: ICIICAP-Universidad Autonoma del Estado de Morelos , Av. Universidad 1001, Col Chamilpa, Cuernavaca, Morelos, México.
authors.forename: Vivechana
authors.initials: V
authors.lastname: Agarwal
authors.affiliation: Institut Charles Gerhardt-UMR5253, Université de Montpellier , F-34095 Montpellier, France.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Mehdi
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Université de Montpellier , 15 Avenue Charles Flahault, Montpellier, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Martinez
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Université de Montpellier , 15 Avenue Charles Flahault, Montpellier, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Subra
authors.affiliation: Laboratoire Charles Coulomb (L2C), UMR 5221 CNRS-Université de Montpellier , Montpellier, France.
authors.forename: Csilla
authors.initials: C
authors.lastname: Gergely
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D001672:Biocompatible Materials; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000737:chemistry*; D015202:Protein Engineering; D012825:Silicon / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D001672:Biocompatible Materials; D010455:Peptides; D012825:Silicon