title: Effects of ethanol, molasses and Lactobacillus plantarum on the fermentation quality, in vitro digestibility and aerobic stability of total mixed ration silages in the Tibetan plateau of China.
abstract: In Tibet, it is common practice to make and relocate total mixed ration (TMR) silages before feeding due to the uneven distribution of forages temporally and spatially. This study was conducted to investigate the effects of Lactobacillus plantarum (L), molasses (M) or ethanol (E) on the fermentation quality and aerobic stability of local adaptive TMR silage. After 45 days of ensiling, pH and ammonia nitrogen in inoculated TMR silages were significantly lower than control. During the first 6 days of the aerobic exposure test, a small fluctuation in lactic acid concentration for all TMR silages was observed, and then silages with ethanol continued this trend, while lactic acid in silage without ethanol sharply decreased until the end of the aerobic exposure period. Meanwhile, pH gradually increased along the aerobic exposure; silages treated with ethanol showed lower pH after 9 days of aerobic exposure. The population of yeast gradually increased during 6 days of aerobic exposure, after that an accelerated rise was observed in TMR silages without ethanol. The combinational beneficial effect of L. plantarum and ethanol was found in combined addition of ethanol and Lactobacillus plantarum silages (EL), indicated by intermediate fermentation quality and higher aerobic stability.
authors.affiliation: Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing, China.
authors.forename: Xianjun
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing, China.|College of Animal Science, Anhui Science and Technology University, Fengyang, China.
authors.forename: Aiyou
authors.initials: A
authors.lastname: Wen
authors.affiliation: Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.affiliation: Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing, China.
authors.forename: Seare T
authors.initials: ST
authors.lastname: Desta
authors.affiliation: Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Shao
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D000641:Ammonia / Q000032:analysis; D002681:China; D004063:Digestion; D000431:Ethanol / Q000494:pharmacology*; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000032:analysis; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D008965:Molasses; D009584:Nitrogen / Q000032:analysis; D012820:Silage / Q000032:analysis / Q000382:microbiology; D018609:Tibet; D013997:Time Factors
chemical_list: D019344:Lactic Acid; D000431:Ethanol; D000641:Ammonia; D009584:Nitrogen
keywords: L. plantarum; TMR silage; aerobic stability; ethanol; fermentation quality