title: Misregulation of the dependence receptor DCC and its upstream lincRNA, LOC100287225, in colorectal cancer.
abstract: BACKGROUND
  Long non-coding RNAs (lncRNAs), a class of regulatory RNAs, play a major role in various cellular processes. Long intergenic non-coding RNAs (lincRNAs), a subclass of lncRNAs, are involved in the trans- and cis-regulation of gene expression. In the case of cis-regulation, by recruiting chromatin-modifying complexes, lincRNAs influence adjacent gene expression.
  
  
  METHODS
  We used quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) to evaluate the coexpression of LOC100287225, a lincRNA, and DCC, one of its adjacent genes that is often decreased in colorectal cancer, in pairs of tumor and adjacent tumor-free tissues of 30 colorectal cancer patients.
  
  
  RESULTS
  The qRT-PCR results revealed the misregulation of these genes during tumorigenesis. Their relative expression levels were significantly lower in tumor tissues than adjacent tumor-free tissues. However, the analysis found no significant correlation between reduced expression of these genes.
  
  
  CONCLUSIONS
  Our study demonstrated the concurrent misregulation of DCC and LOC100287225 in colorectal cancer.
authors.affiliation: Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz - Iran.
authors.forename: Mina
authors.initials: M
authors.lastname: Kazemzadeh
authors.affiliation: Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz - Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Safaralizadeh
authors.affiliation: Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz - Iran.
authors.forename: Mohammad Ali HosseinPour
authors.initials: MA
authors.lastname: Feizi
authors.affiliation: Liver and Gastroenterology Diseases Research Center, Tabriz University of Medical Sciences, Tabriz - Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Somi
authors.affiliation: Department of Pathology, Tabriz University of Medical Sciences, Tabriz - Iran.
authors.forename: Behrooz
authors.initials: B
authors.lastname: Shokoohi
mesh_terms: D000075389:DCC Receptor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D000075389:DCC Receptor; C501909:DCC protein, human; D062085:RNA, Long Noncoding; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins