title: The chemical properties and microbial community characterization of the thermophilic microaerobic pretreatment process.
abstract: Thermophilic microaerobic pretreatment (TMP) was recently reported as an efficient pretreatment method of anaerobic digestion (AD). In this study, the chemical properties and microbial community were characterized to reveal how TMP working. Compared with thermophilic treatment under anaerobic condition (TMP0), cellulase activity obviously improved under microaerobic condition (TMP1), which was 10.9-49.0% higher than that of TMP0. Reducing sugar, SCOD and VFAs concentrations of TMP1 were 2.6-8.9%, 1.8-4.8% and 13.8-24% higher than those of TMP0, respectively. TMP gave obvious rise to phylum Firmicutes, which associated with extracellular enzymes production. The proportion of class Bacilli (belongs to phylum Firmicutes and mainly acts during hydrolysis) in TMP1 was 124.89% higher than that of TMP0, which reflected the greater hydrolytic ability under microaerobic condition. The improved abundance of phylum Firmicutes (especially class Bacilli, order Bacillales) under microaerobic condition could be the fundamental reason for the improved AD performance of thermophilic microaerobic pretreated corn straw.
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Shan-Fei
authors.initials: SF
authors.lastname: Fu
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: He
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China.
authors.forename: Xiao-Shuang
authors.initials: XS
authors.lastname: Shi
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Naveen Reddy
authors.initials: NR
authors.lastname: Katukuri
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Dai
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China. Electronic address: guorb@qibebt.ac.cn.
authors.forename: Rong-Bo
authors.initials: RB
authors.lastname: Guo
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001406:Bacillaceae / Q000502:physiology; D056804:Biofuels; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D044602:Cellulases / Q000737:chemistry / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000378:metabolism; D006868:Hydrolysis; D008697:Methane / Q000096:biosynthesis*; D059013:Microbial Consortia / Q000502:physiology*; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism
chemical_list: D056804:Biofuels; D005232:Fatty Acids, Volatile; D044602:Cellulases; D008697:Methane
keywords: Anaerobic digestion; Cellulase activity; Microbial community structure; Thermophilic microaerobic pretreatment