title: Efficacy of the FilmArray blood culture identification panel for direct molecular diagnosis of infectious diseases from samples other than blood.
abstract: Molecular-based techniques reduce the delay in diagnosing infectious diseases and therefore contribute to better patient outcomes. We assessed the FilmArray blood culture identification (BCID) panel (Biofire Diagnostics/bioMérieux) directly on clinical specimens other than blood: cerebrospinal, joint, pleural and ascitic fluids, bronchoscopy samples and abscesses. We compared the results from 88 samples obtained by culture-based techniques. The percentage of agreement between the two methods was 75 % with a Cohen κ value of 0.51. Global sensitivity and specificity using the FilmArray BCID panel were 71 and 97 %, respectively. Sensitivity was poorer in samples with a low bacterial load, such as ascitic and pleural fluids (25 %), whereas the sensitivity for abscess samples was high (89 %). These findings suggest that the FilmArray BCID panel could be useful to perform microbiological diagnosis directly from samples other than positive blood cultures, as it offers acceptable sensitivity and moderate agreement with conventional microbiological methods. Nevertheless, cost-benefit studies should be performed before introducing this method into algorithms for microbiological diagnostics.
authors.affiliation: Servei de Microbiologia. Hospital de la Santa Creu i Sant Pau Barcelona, Spain.
authors.forename: Miquel
authors.initials: M
authors.lastname: Micó
authors.affiliation: Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015), Instituto de Salud Carlos III, Madrid, Spain.|Servei de Microbiologia. Hospital de la Santa Creu i Sant Pau Barcelona, Spain.|Institut d'Investigació Biomèdica Sant Pau, Barcelona, Spain and Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Ferran
authors.initials: F
authors.lastname: Navarro
authors.affiliation: Servei de Microbiologia. Hospital de la Santa Creu i Sant Pau Barcelona, Spain.
authors.forename: Daniela
authors.initials: D
authors.lastname: de Miniac
authors.affiliation: Servei de Microbiologia. Hospital de la Santa Creu i Sant Pau Barcelona, Spain.
authors.forename: Yésica
authors.initials: Y
authors.lastname: González
authors.affiliation: Servei de Microbiologia. Hospital de la Santa Creu i Sant Pau Barcelona, Spain.
authors.forename: Albert
authors.initials: A
authors.lastname: Brell
authors.affiliation: Servei de Microbiologia. Hospital de la Santa Creu i Sant Pau Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: López
authors.affiliation: Servei de Microbiologia. Hospital de la Santa Creu i Sant Pau Barcelona, Spain.|Institut d'Investigació Biomèdica Sant Pau, Barcelona, Spain and Universitat Autònoma de Barcelona, Barcelona, Spain.|Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Ferran
authors.initials: F
authors.lastname: Sánchez-Reus
authors.affiliation: Servei de Microbiologia. Hospital de la Santa Creu i Sant Pau Barcelona, Spain.|Institut d'Investigació Biomèdica Sant Pau, Barcelona, Spain and Universitat Autònoma de Barcelona, Barcelona, Spain.|Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Mirelis
authors.affiliation: Servei de Microbiologia. Hospital de la Santa Creu i Sant Pau Barcelona, Spain.|Institut d'Investigació Biomèdica Sant Pau, Barcelona, Spain and Universitat Autònoma de Barcelona, Barcelona, Spain.|Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Pere
authors.initials: P
authors.lastname: Coll
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis*; D001826:Body Fluids / Q000382:microbiology*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods; D009181:Mycoses / Q000175:diagnosis*; D015003:Yeasts / Q000302:isolation & purification*