title: [An optimal method for cryopreservation of microamount round spermatids of the mouse].
abstract: OBJECTIVE
  To search for an optimal protocol and freezing conditions for the cryopreservation of microamount round spermatids of the mouse.
  
  
  METHODS
  We compared the survival rates of frozen-thawed microamount round spermatids of the mouse achieved by vitrification or standard slow freezing with different concentrations of glycerol (5, 7, or 9%) and different lengths of equilibrium time (0, 15, 30, 45, or 60 min).
  
  
  RESULTS
  Under the conditions of 7% glycerol and 30 min equilibrium, both vitrification and standard slow freezing achieved high survival rates of spermatids, and the former obtained an even higher rate than the latter ([72.9 ± 15.4]% vs [58.2 ± 17.7]%, P < 0.05).
  
  
  CONCLUSIONS
  A high rate of frozen-thawed microamount round spermatids of the mouse can be achieved by vitrification under the conditions of 7% glycerol and 30 min equilibrium.
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Cun-li
authors.initials: CL
authors.lastname: Wang
mesh_terms: D000818:Animals; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000008:administration & dosage / Q000494:pharmacology; D005990:Glycerol / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D051379:Mice; D013087:Spermatids; D013997:Time Factors; D058989:Vitrification / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D005990:Glycerol