title: Switchable Bioelectrocatalysis Controlled by Dual Stimuli-Responsive Polymeric Interface.
abstract: The engineering of bionanointerfaces using stimuli-responsive polymers offers a new dimension in the design of novel bioelectronic interfaces. The integration of electrode surfaces with stimuli-responsive molecular cues provides a direct control and ability to switch and tune physical and chemical properties of bioelectronic interfaces in various biodevices. Here, we report a dual-responsive biointerface employing a positively responding dual-switchable polymer, poly(NIPAAm-co-DEAEMA)-b-HEAAm, to control and regulate enzyme-based bioelectrocatalysis. The design interface exhibits reversible activation-deactivation of bioelectrocatalytic reactions in response to change in temperature and in pH, which allows manipulation of biomolecular interactions to produce on/off switchable conditions. Using electrochemical measurements, we demonstrate that interfacial bioelectrochemical properties can be tuned over a modest range of temperature (i.e., 20-60 °C) and pH (i.e., pH 4-8) of the medium. The resulting dual-switchable interface may have important implications not only for the design of responsive biocatalysis and on-demand operation of biosensors, but also as an aid to elucidating electron-transport pathways and mechanisms in living organisms by mimicking the dynamic properties of complex biological environments and processes.
authors.affiliation: Biosensors and Bioelectronics Centre, IFM, Linköping University , S-58183 Linköping, Sweden.
authors.forename: Onur
authors.initials: O
authors.lastname: Parlak
authors.affiliation: Biosensors and Bioelectronics Centre, IFM, Linköping University , S-58183 Linköping, Sweden.|Department of Applied Chemistry and Chemical Engineering, University of Dhaka , Dhaka 1000, Bangladesh.
authors.forename: Md
authors.initials: M
authors.lastname: Ashaduzzaman
authors.affiliation: Biosensors and Bioelectronics Centre, IFM, Linköping University , S-58183 Linköping, Sweden.
authors.forename: Suresh B
authors.initials: SB
authors.lastname: Kollipara
authors.affiliation: Biosensors and Bioelectronics Centre, IFM, Linköping University , S-58183 Linköping, Sweden.|Tekidag AB, UCS , Mjärdevi Science Park, Teknikringen 4A, SE 583 30 Linköping, Sweden.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Tiwari
authors.affiliation: Biosensors and Bioelectronics Centre, IFM, Linköping University , S-58183 Linköping, Sweden.
authors.forename: Anthony P F
authors.initials: AP
authors.lastname: Turner
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D055162:Biocatalysis; D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D004563:Electrochemistry; D004566:Electrodes; D004581:Electronics; D004583:Electrons; D004798:Enzymes / Q000737:chemistry; D005296:Ferrous Compounds / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D000075163:Metallocenes; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*; D013696:Temperature
chemical_list: D001672:Biocompatible Materials; D004798:Enzymes; D005296:Ferrous Compounds; D000075163:Metallocenes; D011108:Polymers; C032949:ferrocenecarboxylic acid; D005949:Glucose Oxidase; D005947:Glucose
keywords: ATRP; biointerfaces; stimuli-responsive polymers; switchable bioelectronics; triarm polymers; tunable biocatalysis