title: Interaction between microRNA expression and classical risk factors in the risk of coronary heart disease.
abstract: The aim of this study was to identify the synergistic effect of microRNA expression with classical risk factors of coronary heart disease (CHD) and to explore their diagnostic value for coronary stenotic lesions in subjects with CHD. Plasma samples were obtained from 66 subjects with CHD and from 58 control individuals. A quantitative reverse-transcription PCR (RT-qPCR) assay was conducted to confirm the relative expressions of the known CHD-related miRNAs. The severity of coronary atherosclerosis was based on the Gensini scoring system. The expression of miR-125b in plasma of the CHD group was lower than that of the non-CHD group (0.14 ± 0.09 vs. 0.18 ± 0.10, p = 0.055), and the miR-125b levels significantly decreased following an increasing Gensini score (P = 0.037). Spearman correlation analyses indicated the Gensini score was negatively associated with miR-125b (r = -0.215, p = 0.017). Of all the miRNAs, miR-125b showed the lowest AUC (0.405; 95% CI: 0.305 ~ 0.506, p = 0.070). We found several synergistic effects between miR-125b and classical risk factors, such as age, sex, CR, FBG and HDL-C; the proportion of CHD attributable to the interaction of miR-125b and age was as high as 80%. Therefore, miR-125b was shown to play an important role in individual's susceptibility to developing CHD.
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Ding
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Peng-Cheng
authors.initials: PC
authors.lastname: Ge
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Kangda school, Nanjing Medical University, Lianyungang 222000, Jiangsu Province, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Jia
authors.affiliation: Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Friendship Hospital of Ili Kazakh Autonomous Prefecture, Yining 835000, Xinjiang, China.
authors.forename: Feng-Hui
authors.initials: FH
authors.lastname: An
authors.affiliation: Friendship Hospital of Ili Kazakh Autonomous Prefecture, Yining 835000, Xinjiang, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Li
authors.affiliation: Friendship Hospital of Ili Kazakh Autonomous Prefecture, Yining 835000, Xinjiang, China.
authors.forename: Zhao-Hong
authors.initials: ZH
authors.lastname: Chen
authors.affiliation: Friendship Hospital of Ili Kazakh Autonomous Prefecture, Yining 835000, Xinjiang, China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Mao
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Zhao-Yang
authors.initials: ZY
authors.lastname: Li
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gu
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Tie-Bing
authors.initials: TB
authors.lastname: Zhu
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Chun-Jian
authors.initials: CJ
authors.lastname: Li
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Lian-Sheng
authors.initials: LS
authors.lastname: Wang
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Wen-Zhu
authors.initials: WZ
authors.lastname: Ma
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Yang
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: En-Zhi
authors.initials: EZ
authors.lastname: Jia
mesh_terms: D000367:Age Factors; D000368:Aged; D019540:Area Under Curve; D001786:Blood Glucose / Q000378:metabolism; D016022:Case-Control Studies; D008076:Cholesterol, HDL / Q000097:blood; D003324:Coronary Artery Disease / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D003327:Coronary Disease / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D003404:Creatinine / Q000097:blood; D005215:Fasting; D005260:Female; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D011446:Prospective Studies; D012372:ROC Curve; D012307:Risk Factors; D012720:Severity of Illness Index; D012737:Sex Factors
chemical_list: D001786:Blood Glucose; D008076:Cholesterol, HDL; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D003404:Creatinine