title: [Protective effect of salidroside against high altitude hypoxia-induced brain injury in rats].
abstract: OBJECTIVE
  To observe the protective effect of salidroside against brain injury in rats exposed to hypobaric hypoxia, and investigate the molecular mechanism of salidroside in the prevention of hypobaric hypoxia-induced brain injury.
  
  
  METHODS
  Rats were placed in experiment module simulating 6000 m altitude to establish acute hypobaric hypoxia-induced brain injury models. Their respiratory frequency was observed and recorded. Cell apoptosis in the hippocampal dentate gyrus (DG) was detected by TUNEL assay; the expressions of Ras homolog family member A (RhoA), phosphorylated extracellular signal-regulated kinase (p-ERK) and phosphorylated c-Jun N-terminal kinase (p-JNK) were detected by Western blotting.
  
  
  RESULTS
  After acute exposure to 6000 m altitude, the respiratory frequency of the rats increased remarkably. The simulation of hypobaric hypoxia induced cell apoptosis in hippocampal DG region, and salidroside intervention inhibited the process of cell apoptosis. The expressions of RhoA, p-ERK, p-JNK decreased after hypobaric hypoxia exposure. Salidroside intervention reversed RhoA expression and raised the levels of p-ERK and p-JNK.
  
  
  CONCLUSIONS
  Acute exposure to hypobaric hypoxia can induce cell apoptosis in rat hippocampal DG, and salidroside can protect the cells from the exposure-induced apoptosis.
authors.affiliation: Department of Occupational and Environmental Health, Ministry of Education Key Laboratory of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xiaoru
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Occupational and Environmental Health, Ministry of Education Key Laboratory of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xiangnan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Occupational and Environmental Health, Ministry of Education Key Laboratory of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Occupational and Environmental Health, Ministry of Education Key Laboratory of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Occupational and Environmental Health, Ministry of Education Key Laboratory of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jiye
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Occupational and Environmental Health, Ministry of Education Key Laboratory of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Meng
authors.affiliation: Department of Occupational and Environmental Health, Ministry of Education Key Laboratory of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Luo
authors.affiliation: Department of Occupational and Environmental Health, Ministry of Education Key Laboratory of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000532:Altitude Sickness / Q000188:drug therapy* / Q000473:pathology; D000818:Animals; D017209:Apoptosis; D019610:Cytoprotection; D018891:Dentate Gyrus / Q000473:pathology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005960:Glucosides / Q000627:therapeutic use*; D002534:Hypoxia, Brain / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D010636:Phenols / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012119:Respiration; D020742:rhoA GTP-Binding Protein / Q000032:analysis
chemical_list: D005960:Glucosides; D010636:Phenols; D048049:Extracellular Signal-Regulated MAP Kinases; D020742:rhoA GTP-Binding Protein; C009172:rhodioloside