title: Simplified Protocol for Carba NP Test for Enhanced Detection of Carbapenemase Producers Directly from Bacterial Cultures.
abstract: We compared carbapenemase detection among 266 Gram-negative bacilli (161 carbapenemase producers) using the Carba NP tests issued by the CLSI (CNPt-CLSI) and a novel protocol (CNPt-direct) designed for carbapenemase detection direct from bacterial cultures (instead of bacterial extracts required by the CLSI tests). The specificities were comparable (100%), but the CNPt-direct was more sensitive (98% versus 84%). The CNPt-direct was easier to perform due to the direct use of colonies and offered a more robust detection of carbapenemase producers.
authors.affiliation: Servicio Antimicrobianos, Laboratorio Nacional y Regional de Referencia en Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas (INEI), ANLIS Dr. Carlos G. Malbrán, Ministerio de Salud de la Nación, Buenos Aires, Argentina.
authors.forename: Fernando
authors.initials: F
authors.lastname: Pasteran
authors.affiliation: Public Health Ontario Laboratories (PHOL), Toronto, Ontario, Canada.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Tijet
authors.affiliation: Public Health Ontario Laboratories (PHOL), Toronto, Ontario, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada Department of Microbiology, Mt. Sinai Hospital, Toronto, Ontario, Canada.
authors.forename: Roberto G
authors.initials: RG
authors.lastname: Melano
authors.affiliation: Servicio Antimicrobianos, Laboratorio Nacional y Regional de Referencia en Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas (INEI), ANLIS Dr. Carlos G. Malbrán, Ministerio de Salud de la Nación, Buenos Aires, Argentina acorso@anlis.gov.ar.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Corso
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000379:methods*; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000302:isolation & purification*; D006801:Humans; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase