title: Isolation, purification, and antioxidant activities of degraded polysaccharides from Enteromorpha prolifera.
abstract: In the present study, purification and characterization of enzymatic hydrolysates of polysaccharide from Enteromorpha prolifera (HPE) are described. HPE was sequentially purified by DEAE Cellulose-52 chromatography and Sephadex G-100 chromatography to afford three fractions, namely, PHPE1, PHPE2, and PHPE3. Molecular weights of these three fractions were measured to be 103, 45.4, and 9.8kDa, respectively, using high performance gel permeation chromatography (HPGPC). The three fractions were evaluated for their antioxidant activities by determining their ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and superoxide anion radicals. PHPE2 was found to possess the strongest scavenging ability. GC-MS analysis indicates that PHPE2 is mainly composed of mannose, xylose, and glucose.
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Xu
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China.
authors.forename: Qin-Wei
authors.initials: QW
authors.lastname: Zhou
authors.affiliation: Department of Food processing and Safety Control, South China Sea Fishery Research Institute, Guangzhou, PR China.
authors.forename: Shu-Xian
authors.initials: SX
authors.lastname: Hao
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China. Electronic address: taozhou@zjgsu.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Applied Chemistry, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China. Electronic address: hjxie@zjgsu.edu.cn.
authors.forename: Hu-Jun
authors.initials: HJ
authors.lastname: Xie
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D016166:Free Radical Scavengers / Q000494:pharmacology; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000737:chemistry; D008970:Molecular Weight; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D013481:Superoxides / Q000737:chemistry; D044445:Ulva / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011134:Polysaccharides; D013481:Superoxides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant activity; Enteromorpha prolifera; Polysaccharides