title: Beyond the Linear Genome: Paired-End Sequencing as a Biophysical Tool.
abstract: Paired-end sequencing has enabled a variety of new methods for high-throughput interrogation of both genome structure and chromatin architecture. Here, we discuss how the paired-end paradigm can be used to interpret sequencing data as biophysical measurements of in vivo chromatin structure that report on single molecules in single cells.
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, CA, 94305, USA.
authors.forename: Viviana I
authors.initials: VI
authors.lastname: Risca
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, CA, 94305, USA; Department of Applied Physics, Stanford University, Stanford, CA, 94305, USA. Electronic address: wjg@stanford.edu.
authors.forename: William J
authors.initials: WJ
authors.lastname: Greenleaf
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods* / Q000639:trends; D016678:Genome / Q000235:genetics*; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods* / Q000639:trends
keywords: chromatin; chromosome structure; in vivo measurements; paired-end sequencing