title: Lipid droplet distribution of immature canine oocytes in relation to their size and the reproductive stage.
abstract: This study investigated the distribution of lipid droplets (LD) in immature canine oocytes in relation to their size and the reproductive stage. Oocytes were collected from the ovaries of bitches at different estrous stages, divided according to their size (110-120 µm; >120 µm), and stained with Nile Red to detect lipid droplet distribution. At the follicular phase most of the oocytes displayed a diffuse pattern of LD distribution, whereas at anestrus and luteal phase oocytes showed LD mainly in a peripheral/ perinuclear LD distribution. A significantly higher intensity of LD has been recorded in the oocytes > 120 µm compared to those of smaller size (110 - 120 µm) at all stages of the estrous cycle. At follicular phase, oocytes > 120 µm displayed LD intensity similar to that of oocytes > 120 µm at luteal phase and higher compared to the oocytes of the other groups.
authors.affiliation: Department of Veterinary Medicine, Section of Obstetrics and Gynecology, University of Sassari, Sassari, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Ariu
authors.affiliation: Department of Veterinary Medicine, Section of Obstetrics and Gynecology, University of Sassari, Sassari, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Strina
authors.affiliation: Department of Veterinary Medicine, Section of Obstetrics and Gynecology, University of Sassari, Sassari, Italy.
authors.forename: Ombretta
authors.initials: O
authors.lastname: Murrone
authors.affiliation: Department of Veterinary Medicine, Section of Obstetrics and Gynecology, University of Sassari, Sassari, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Falchi
authors.affiliation: Department of Veterinary Medicine, Section of Obstetrics and Gynecology, University of Sassari, Sassari, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Bebbere
authors.affiliation: Department of Veterinary Medicine, Section of Obstetrics and Gynecology, University of Sassari, Sassari, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Ledda
authors.affiliation: Department of Veterinary Medicine, Section of Obstetrics and Gynecology, University of Sassari, Sassari, Italy.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Zedda
authors.affiliation: Department of Veterinary Medicine, Section of Obstetrics and Gynecology, University of Sassari, Sassari, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Pau
authors.affiliation: Department of Veterinary Medicine, Section of Obstetrics and Gynecology, University of Sassari, Sassari, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Bogliolo
mesh_terms: D000780:Anestrus / Q000378:metabolism; D000818:Animals; D048429:Cell Size; D004285:Dogs / Q000378:metabolism* / Q000502:physiology*; D030762:Estrous Cycle / Q000378:metabolism*; D005260:Female; D005498:Follicular Phase / Q000378:metabolism*; D066292:Lipid Droplets / Q000378:metabolism*; D008183:Luteal Phase / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism* / Q000502:physiology*; D009866:Oogenesis / Q000502:physiology*; D010078:Oxazines; D013194:Staining and Labeling
chemical_list: D010078:Oxazines; C044808:nile red
keywords: Nile Red; diameter; estrous cycle; lipids; oocyte