title: Motility of liquid stored ram spermatozoa is altered by dilution rate independent of seminal plasma concentration.
abstract: The fertility after use of liquid stored ram semen following cervical AI rapidly decreases if semen is stored beyond 12h. The dilution of seminal plasma is often cited as a key contributor to the diminished motility and fertility of ram spermatozoa subjected to liquid preservation. Two experiments were conducted to assess the effect of spermatozoa concentration (i.e. dilution rate) and percentage of seminal plasma on the motility and viability of liquid stored ram spermatozoa. In Experiment 1, semen was diluted to one of seven concentrations ranging from 0.2 to 1.4×10(9)spermatozoa/ml with milk and assessed for motility after 3 or 24h of storage at 15°C. In Experiment 2, semen was collected and washed to remove seminal plasma before re-dilution to 0.2-1.4×10(9)spermatozoa/ml with milk containing 0%, 20% or 40% (final v/v ratio) seminal plasma and assessed for viability and motility after 3 or 24h of storage at 15°C. Whereas motility was not affected by spermatozoa concentration after 3h of storage, the proportion of progressive spermatozoa decreased after 24h of storage when spermatozoa concentration was greater than 1.0×10(9)spermatozoa/ml. The duration of preservation and the spermatozoa concentration affected spermatozoa motility but had no impact on spermatozoa viability. This negative effect of greater spermatozoa concentrations on motility was independent of the presence and the concentration of seminal plasma. The seminal plasma at both concentrations (20% and 40%) had a protective effect on spermatozoa motility after 24h of storage. These findings have the potential to improve the efficiency of cervical AI with liquid stored ram semen.
authors.affiliation: INDEGSAL, University of León, 24071 León, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Mata-Campuzano
authors.affiliation: INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly, France.
authors.forename: C
authors.initials: C
authors.lastname: Soleilhavoup
authors.affiliation: INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly, France.
authors.forename: G
authors.initials: G
authors.lastname: Tsikis
authors.affiliation: INDEGSAL, University of León, 24071 León, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Martinez-Pastor
authors.affiliation: Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia.
authors.forename: S P
authors.initials: SP
authors.lastname: de Graaf
authors.affiliation: INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly, France. Electronic address: xavier.druart@tours.inra.fr.
authors.forename: X
authors.initials: X
authors.lastname: Druart
mesh_terms: D000818:Animals; D008297:Male; D012661:Semen / Q000502:physiology; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012756:Sheep; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology
keywords: AI; Preservation; Ram semen