title: Novel imidazolium-embedded N,N-dimethylaminopropyl-functionalized silica-based stationary phase for hydrophilic interaction/reversed-phase mixed-mode chromatography.
abstract: A novel imidazolium-embedded N,N-dimethylaminopropyl-functionalized silica-based stationary phase (Sil-ImCl) was prepared and further used for hydrophilic interaction/reversed-phase mixed-mode chromatography. The Sil-ImCl stationary phase was respectively characterized by Fourier transform infrared spectrometry, thermogravimetric analysis, and element analysis. A variety of hydrophilic or hydrophobic compounds were used to evaluate the retention mechanisms of the developed stationary phase, and the effects of buffer salt concentration and pH of mobile phase on the retention of these compounds were also investigated. The developed stationary phase was successfully applied for separation of nucleosides and nucleic acid bases, water-soluble vitamins, phenols, and positional isomers. Moreover, simultaneous separation of polar and nonpolar compounds was also achieved with high resolution, outperforming the commercially available C8 column and amino column. Furthermore, the Sil-ImCl stationary phase has been successfully applied for separation of secondary metabolites of Hansfordia sinuosae. All these results demonstrate that the Sil-ImCl stationary phase might be promising for separation of complex polar and nonpolar compounds with high efficiency, especially in biological industry.
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China.
authors.forename: Shijia
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Xu
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China.
authors.forename: Danyang
authors.initials: D
authors.lastname: Li
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China.
authors.forename: Mingyan
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China. xiaoqiao@hbu.edu.cn.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Qiao
authors.affiliation: College of Chemistry & Environmental Science, Hebei University, Baoding, 071002, China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Qin
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China.
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Yan
mesh_terms: D056148:Chromatography, Reverse-Phase / Q000295:instrumentation* / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D007536:Isomerism; D009696:Nucleic Acids / Q000737:chemistry / Q000302:isolation & purification; D009705:Nucleosides / Q000737:chemistry / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D009696:Nucleic Acids; D009705:Nucleosides; D012822:Silicon Dioxide
keywords: Hydrophilic interaction liquid chromatography; Imidazolium-embedded N,N-dimethylaminopropyl-functionalized stationary phase; Mixed-mode separation; Reversed-phase liquid chromatography