title: Synergistic action of recombinant accessory hemicellulolytic and pectinolytic enzymes to Trichoderma reesei cellulase on rice straw degradation.
abstract: Synergism between core cellulases and accessory hydrolytic/non-hydrolytic enzymes is the basis of efficient hydrolysis of lignocelluloses. In this study, the synergistic action of three recombinant accessory enzymes, namely GH62 α-l-arabinofuranosidase (ARA), CE8 pectin esterase (PET), and GH10 endo-1,4-beta-xylanase (XYL) from Aspergillus aculeatus expressed in Pichia pastoris to a commercial Trichoderma reesei cellulase (Accellerase® 1500; ACR) on hydrolysis of alkaline pretreated rice straw was studied using a mixture design approach. Applying the full cubic model, the optimal ratio of quaternary enzyme mixture was predicted to be ACR:ARA:PET:XYL of 0.171:0.079:0.100:0.150, which showed a glucose releasing efficiency of 0.173 gglc/FPU, higher than the binary ACR:XYL mixture (0.122 gglc/FPU) and ACR alone (0.081 gglc/FPU) leading to a 47.3% increase in glucose yield compared with that from ACR at the same cellulase dosage. The result demonstrates the varying degree of synergism of accessory enzymes to cellulases useful for developing tailor-made enzyme systems for bio-industry.
authors.affiliation: Enzyme Technology Laboratory and Integrative Biorefinery Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Thailand Science Park, 113 Pahonyothin Road, Pathumthani 12120, Thailand.
authors.forename: Thanaporn
authors.initials: T
authors.lastname: Laothanachareon
authors.affiliation: Enzyme Technology Laboratory and Integrative Biorefinery Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Thailand Science Park, 113 Pahonyothin Road, Pathumthani 12120, Thailand.
authors.forename: Benjarat
authors.initials: B
authors.lastname: Bunterngsook
authors.affiliation: Enzyme Technology Laboratory and Integrative Biorefinery Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Thailand Science Park, 113 Pahonyothin Road, Pathumthani 12120, Thailand.
authors.forename: Surisa
authors.initials: S
authors.lastname: Suwannarangsee
authors.affiliation: Enzyme Technology Laboratory and Integrative Biorefinery Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Thailand Science Park, 113 Pahonyothin Road, Pathumthani 12120, Thailand.
authors.forename: Lily
authors.initials: L
authors.lastname: Eurwilaichitr
authors.affiliation: Enzyme Technology Laboratory and Integrative Biorefinery Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Thailand Science Park, 113 Pahonyothin Road, Pathumthani 12120, Thailand. Electronic address: verawat@biotec.or.th.
authors.forename: Verawat
authors.initials: V
authors.lastname: Champreda
mesh_terms: D001230:Aspergillus / Q000201:enzymology; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism; D044602:Cellulases / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000235:genetics / Q000378:metabolism; D004798:Enzymes / Q000235:genetics / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D012275:Oryza / Q000378:metabolism*; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D004798:Enzymes; D011994:Recombinant Proteins; C036909:lignocellulose; D008031:Lignin; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D044602:Cellulases; D006026:Glycoside Hydrolases; D002480:Cellulase; C019713:alpha-N-arabinofuranosidase; D043364:Endo-1,4-beta Xylanases
keywords: Accessory enzymes; Aspergillus aculeatus; Cellulase; Lignocellulose; Mixture design