title: Phylogenetic analysis of Indian rabies virus isolates targeting the complete glycoprotein gene.
abstract: Rabies a fatal viral zoonosis is endemic in India. There is no report on phylogenetic study of Indian rabies virus isolates based on the complete G gene. In the present study, a total of 25 rabies positive brain samples collected during 2001-2014 from North India (UP, MP, Delhi, Rajasthan), South India (Kerala and Karnataka) and Gujarat states belonging to six different host species were subjected to G gene amplification by RT-PCR as three overlapping fragments of 881 bp, 991 bp and 618 bp. Phylogenetic analysis revealed that all Indian rabies virus isolates are genetically closely related with Arctic-like 1a lineage viruses. However, two distinct clusters were identified namely, India South and India North. All the Indian rabies isolates had 95.5-100% homology related to geography, but not to host species. Deduced amino acids on comparison revealed two amino acid changes, aa 356 in ECTO; N→K and aa 458; M→I, which were found to distinguish between the India South and India North isolates.
authors.affiliation: Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P. 243 122, India. Electronic address: cpasusan18@gmail.com.
authors.forename: Susan
authors.initials: S
authors.lastname: Cherian
authors.affiliation: Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P. 243 122, India. Electronic address: rajendra_singh5747@rediffmail.com.
authors.forename: Rajendra
authors.initials: R
authors.lastname: Singh
authors.affiliation: CARDRAD, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P. 243 122, India.
authors.forename: K P
authors.initials: KP
authors.lastname: Singh
authors.affiliation: NIVEDI, Bengaluru, Karnataka 560 064, India.
authors.forename: G B
authors.initials: GB
authors.lastname: Manjunatha Reddy
authors.affiliation: Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P. 243 122, India.
authors.lastname: Anjaneya
authors.affiliation: Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P. 243 122, India.
authors.forename: G V P P S
authors.initials: GVPPS
authors.lastname: Ravi Kumar
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary And Animal Sciences, Pookot, Wayanad, Kerala 673 576, India.
authors.forename: T G
authors.initials: TG
authors.lastname: Sumithra
authors.affiliation: Division of Biological Products, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P. 243 122, India.
authors.forename: R P
authors.initials: RP
authors.lastname: Singh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D007194:India / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D058974:Phylogeography; D012367:RNA, Viral; D011817:Rabbits; D011818:Rabies / Q000453:epidemiology / Q000821:virology*; D011820:Rabies virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*; D015047:Zoonoses / Q000453:epidemiology / Q000821:virology
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C064951:glycoprotein G, Rabies virus
keywords: G gene; Indian isolate; Phylogenetic analysis; Rabies virus; Wildlife species