title: Identification of single nucleotide polymorphisms in the ASB15 gene and their associations with chicken growth and carcass traits.
abstract: ASB15 is a member of the ankyrin repeat and suppressor of cytokine signaling box family, and is predominantly expressed in skeletal muscle. In the present study, an F2 resource population of Gushi chickens crossed with Anka broilers was used to investigate the genetic effects of the chicken ASB15 gene. Two single nucleotide polymorphisms (SNPs) (rs315759231 A>G and rs312619270 T>C) were identified in exon 7 of the ASB15 gene using forced chain reaction-restriction fragment length polymorphism and DNA sequencing. One was a missense SNP (rs315759231 A>G) and the other was a synonymous SNP (rs312619270 T>C). The rs315759231 A>G polymorphism was significantly associated with body weight at birth, 12-week body slanting length, semi-evisceration weight, evisceration weight, leg muscle weight, and carcass weight (P < 0.05). The rs312619270 T>C polymorphism was significantly associated with body weight at birth, 4, 8, and 12-week body weight, 8-week shank length, 12-week breast bone length, 8 and 12-week body slanting length, breast muscle weight, and carcass weight (P < 0.05). Our results suggest that the ASB15 gene profoundly affects chicken growth and carcass traits.
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
authors.forename: Y C
authors.initials: YC
authors.lastname: Wang
authors.affiliation: College of Animal Husbandry and Veterinary Engineering, Henan Agricultural University, Zhengzhou, China.
authors.forename: R R
authors.initials: RR
authors.lastname: Jiang
authors.affiliation: College of Animal Husbandry and Veterinary Engineering, Henan Agricultural University, Zhengzhou, China.
authors.forename: X T
authors.initials: XT
authors.lastname: Kang
authors.affiliation: College of Animal Husbandry and Veterinary Engineering, Henan Agricultural University, Zhengzhou, China.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: College of Animal Husbandry and Veterinary Engineering, Henan Agricultural University, Zhengzhou, China.
authors.forename: R L
authors.initials: RL
authors.lastname: Han
authors.affiliation: College of Animal Husbandry and Veterinary Engineering, Henan Agricultural University, Zhengzhou, China.
authors.forename: J
authors.initials: J
authors.lastname: Geng
authors.affiliation: College of Animal Husbandry and Veterinary Engineering, Henan Agricultural University, Zhengzhou, China.
authors.forename: J X
authors.initials: JX
authors.lastname: Fu
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
authors.forename: J P
authors.initials: JP
authors.lastname: Wu
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens / Q000235:genetics* / Q000254:growth & development*; D004587:Electrophoresis, Agar Gel; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D060005:Genotyping Techniques; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D008460:Meat; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D017422:Sequence Analysis, DNA
chemical_list: D030161:Avian Proteins