title: The influence of type 2 diabetes mellitus on salivary matrix metalloproteinase-8 levels and periodontal parameters: A study in an Indian population.
abstract: OBJECTIVE
  Although many studies reported more severe periodontal disease and the existing proinflammatory conditions in patients with diabetes but only few have examined the effect of type 2 diabetes mellitus (DM) on salivary matrix metalloproteinase-8 (MMP-8) level and other periodontal parameters. This study aims to evaluate the effect of type 2 DM on salivary MMP-8 levels and periodontal parameters, which might be useful in monitoring periodontal disease in diabetes.
  
  
  METHODS
  A total of 90 subjects were selected for the study and were divided into three groups: Group I included 30 healthy subjects; Group II included 30 subjects without type 2 DM but with chronic periodontitis, and Group III included 30 subjects with type 2 DM and chronic periodontitis. Periodontal parameters such as plaque index (PI), gingival index (GI), pocket probing depth (PPD), and clinical attachment level (CAL) were taken. The salivary MMP-8 level was estimated by Quantikine Human total MMP-8 immunoassay kit using ELISA method.
  
  
  RESULTS
  The mean value of the salivary MMP-8 of Group III was highest followed by Group II and Group I, the least. The other periodontal parameters PI, GI, PPD, CAL, was comparatively highest for Group III.
  
  
  CONCLUSIONS
  This study suggests that diabetes is associated with an increased prevalence, extent, and severity of periodontitis. Furthermore, the increased levels of MMP-8 indicate the influence of diabetes on their salivary concentration.
authors.affiliation: Department of Periodontics and Community Dentistry, Dr. Z. A. Dental College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
authors.forename: Namita
authors.initials: N
authors.lastname: Gupta
authors.affiliation: Department of Periodontics and Community Dentistry, Dr. Z. A. Dental College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
authors.forename: Narinder Dev
authors.initials: ND
authors.lastname: Gupta
authors.affiliation: Department of Biochemistry, Subharti Medical College, Meerut, Uttar Pradesh, India.
authors.forename: Akash
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Department of Periodontics and Community Dentistry, Dr. Z. A. Dental College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
authors.forename: Lata
authors.initials: L
authors.lastname: Goyal
authors.affiliation: Department of Periodontics, Tamil Nadu Government Medical College, Chennai, Tamil Nadu, India.
authors.forename: Sagar
authors.initials: S
authors.lastname: Garg
keywords: Chronic periodontitis; matrix metalloproteinase-8; periodontal parameters; saliva; type 2 diabetes mellitus