title: Differential protein expression analysis following olfactory learning in Apis cerana.
abstract: Studies of olfactory learning in honeybees have helped to elucidate the neurobiological basis of learning and memory. In this study, protein expression changes following olfactory learning in Apis cerana were investigated using isobaric tags for relative and absolute quantification (iTRAQ) technology. A total of 2406 proteins were identified from the trained and untrained groups. Among these proteins, 147 were differentially expressed, with 87 up-regulated and 60 down-regulated in the trained group compared with the untrained group. These results suggest that the differentially expressed proteins may be involved in the regulation of olfactory learning and memory in A. cerana. The iTRAQ data can provide information on the global protein expression patterns associated with olfactory learning, which will facilitate our understanding of the molecular mechanisms of learning and memory of honeybees.
authors.affiliation: Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China.
authors.forename: Li-Zhen
authors.initials: LZ
authors.lastname: Zhang
authors.affiliation: Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China.
authors.forename: Wei-Yu
authors.initials: WY
authors.lastname: Yan
authors.affiliation: Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China.
authors.forename: Zi-Long
authors.initials: ZL
authors.lastname: Wang
authors.affiliation: Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China.
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Guo
authors.affiliation: Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yi
authors.affiliation: Research School of Biology, Australian National University, Canberra, ACT, 0200, Australia.
authors.forename: Shao-Wu
authors.initials: SW
authors.lastname: Zhang
authors.affiliation: Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China. bees1965@sina.com.
authors.forename: Zhi-Jiang
authors.initials: ZJ
authors.lastname: Zeng
mesh_terms: D000818:Animals; D001516:Bees / Q000378:metabolism*; D020869:Gene Expression Profiling; D019476:Insect Proteins / Q000378:metabolism*; D007858:Learning / Q000502:physiology*; D009483:Neuropsychological Tests; D055696:Olfactory Perception / Q000502:physiology*; D010812:Physical Stimulation; D011897:Random Allocation
chemical_list: D019476:Insect Proteins
keywords: Apis cerana; Isobaric tags for relative and absolute quantification (iTRAQ); Learning and memory; Proboscis extension response