title: Reactive oxygen species and mitochondria: A nexus of cellular homeostasis.
abstract: Reactive oxygen species (ROS) are integral components of multiple cellular pathways even though excessive or inappropriately localized ROS damage cells. ROS function as anti-microbial effector molecules and as signaling molecules that regulate such processes as NF-kB transcriptional activity, the production of DNA-based neutrophil extracellular traps (NETs), and autophagy. The main sources of cellular ROS are mitochondria and NADPH oxidases (NOXs). In contrast to NOX-generated ROS, ROS produced in the mitochondria (mtROS) were initially considered to be unwanted by-products of oxidative metabolism. Increasing evidence indicates that mtROS have been incorporated into signaling pathways including those regulating immune responses and autophagy. As metabolic hubs, mitochondria facilitate crosstalk between the metabolic state of the cell with these pathways. Mitochondria and ROS are thus a nexus of multiple pathways that determine the response of cells to disruptions in cellular homeostasis such as infection, sterile damage, and metabolic imbalance. In this review, we discuss the roles of mitochondria in the generation of ROS-derived anti-microbial effectors, the interplay of mitochondria and ROS with autophagy and the formation of DNA extracellular traps, and activation of the NLRP3 inflammasome by ROS and mitochondria.
authors.affiliation: Department of Biochemistry, University of Geneva, 30 quai Ernest Ansermet, Sciences II, CH-1211 Genève-4, Switzerland. Electronic address: joedan.dunn@unige.ch.
authors.forename: Joe
authors.initials: J
authors.lastname: Dan Dunn
authors.affiliation: National Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin 12, Ireland. Electronic address: luis.alvarez@ucd.ie.
authors.forename: Luis Aj
authors.initials: LA
authors.lastname: Alvarez
authors.affiliation: Department of Biochemistry, University of Geneva, 30 quai Ernest Ansermet, Sciences II, CH-1211 Genève-4, Switzerland. Electronic address: Xuezhi.Zhang@unige.ch.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry, University of Geneva, 30 quai Ernest Ansermet, Sciences II, CH-1211 Genève-4, Switzerland. Electronic address: Thierry.Soldati@unige.ch.
authors.forename: Thierry
authors.initials: T
authors.lastname: Soldati
mesh_terms: D000818:Animals; D001343:Autophagy; D065206:Extracellular Traps / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D058847:Inflammasomes; D017382:Reactive Oxygen Species
keywords: Autophagy; Immunity; Inflammasome; Mitochondria; Neutrophil extracellular traps; Reactive oxygen species