title: Nucleocytoplasmic Transport of RNAs and RNA-Protein Complexes.
abstract: RNAs and ribonucleoprotein complexes (RNPs) play key roles in mediating and regulating gene expression. In eukaryotes, most RNAs are transcribed, processed and assembled with proteins in the nucleus and then either function in the cytoplasm or also undergo a cytoplasmic phase in their biogenesis. This compartmentalization ensures that sequential steps in gene expression and RNP production are performed in the correct order and it allows important quality control mechanisms that prevent the involvement of aberrant RNAs/RNPs in these cellular pathways. The selective exchange of RNAs/RNPs between the nucleus and cytoplasm is enabled by nuclear pore complexes, which function as gateways between these compartments. RNA/RNP transport is facilitated by a range of nuclear transport receptors and adaptors, which are specifically recruited to their cargos and mediate interactions with nucleoporins to allow directional translocation through nuclear pore complexes. While some transport factors are only responsible for the export/import of a certain class of RNA/RNP, others are multifunctional and, in the case of large RNPs, several export factors appear to work together to bring about export. Recent structural studies have revealed aspects of the mechanisms employed by transport receptors to enable specific cargo recognition, and genome-wide approaches have provided the first insights into the diverse composition of pre-mRNPs during export. Furthermore, the regulation of RNA/RNP export is emerging as an important means to modulate gene expression under stress conditions and in disease.
authors.affiliation: Institute for Molecular Biology, Goettingen University Medical Department, 37073 Goettingen, Germany.
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Sloan
authors.affiliation: Laboratoire de Biologie Moléculaire Eucaryote, UMR 5099, Université de Toulouse-Paul Sabatier, CNRS, Toulouse, France.
authors.forename: Pierre-Emmanuel
authors.initials: PE
authors.lastname: Gleizes
authors.affiliation: Institute for Molecular Biology, Goettingen University Medical Department, 37073 Goettingen, Germany; Goettingen Centre for Molecular Biosciences, Georg-August-University, 37075 Goettingen, Germany. Electronic address: Markus.Bohnsack@med.uni-goettingen.de.
authors.forename: Markus T
authors.initials: MT
authors.lastname: Bohnsack
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D022022:Nuclear Pore / Q000378:metabolism / Q000502:physiology; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism*
chemical_list: D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D012313:RNA
keywords: RNA–protein complex; RNP biogenesis; export; import; nuclear pore complex