title: Antimicrobial activity of Alcaligenes sp. HPC 1271 against multidrug resistant bacteria.
abstract: Alcaligenes sp. HPC 1271 demonstrated antibacterial activity against multidrug resistant bacteria, Enterobacter sp., resistant to sulfamethoxazole, ampicillin, azithromycin, and tetracycline, as well as against Serratia sp. GMX1, resistant to the same antibiotics with the addition of netilmicin. The cell-free culture supernatant was analyzed for possible antibacterials by HPLC, and the active fraction was further identified by LC-MS. Results suggest the production of tunicamycin, a nucleoside antibiotic. The draft genome of this bacterial isolate was analyzed, and the 4.2 Mb sequence data revealed six secondary metabolite-producing clusters, identified using antiSMASH platform as ectoine, butyrolactone, phosphonate, terpene, polyketides, and nonribosomal peptide synthase (NRPS). Additionally, the draft genome demonstrated homology to the tunicamycin-producing gene cluster and also defined 30 ORFs linked to protein secretion that could also play a role in the antibacterial activity observed. Gene expression analysis demonstrated that both NRPS and dTDP-glucose 4,6-dehydratase gene clusters are functional and could be involved in antibacterial biosynthesis.
authors.affiliation: CSIR-NEERI, Nehru Marg, Nagpur, 440020, Maharashtra, India. a_kapley@neeri.res.in.|Environmental Genomics Division, National Environmental Engineering Research Institute, CSIR, Nehru Marg, Nagpur, 440 020, India. a_kapley@neeri.res.in.
authors.forename: Atya
authors.initials: A
authors.lastname: Kapley
authors.affiliation: CSIR-NEERI, Nehru Marg, Nagpur, 440020, Maharashtra, India.
authors.forename: Himgouri
authors.initials: H
authors.lastname: Tanksale
authors.affiliation: CSIR-NEERI, Nehru Marg, Nagpur, 440020, Maharashtra, India.
authors.forename: Sneha
authors.initials: S
authors.lastname: Sagarkar
authors.affiliation: CSIR-IICT, Uppal Road, Tarnaka, Hyderabad, 500007, Andhra Pradesh, India.
authors.forename: A R
authors.initials: AR
authors.lastname: Prasad
authors.affiliation: CSIR-IICT, Uppal Road, Tarnaka, Hyderabad, 500007, Andhra Pradesh, India.
authors.forename: Rathod Aravind
authors.initials: RA
authors.lastname: Kumar
authors.affiliation: CSIR-NEERI, Nehru Marg, Nagpur, 440020, Maharashtra, India.
authors.forename: Nandita
authors.initials: N
authors.lastname: Sharma
authors.affiliation: CSIR-NEERI, Nehru Marg, Nagpur, 440020, Maharashtra, India.
authors.forename: Asifa
authors.initials: A
authors.lastname: Qureshi
authors.affiliation: CSIR-NEERI, Nehru Marg, Nagpur, 440020, Maharashtra, India.
authors.forename: Hemant J
authors.initials: HJ
authors.lastname: Purohit
mesh_terms: D000421:Alcaligenes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000599:Amino Acids, Diamino / Q000378:metabolism; D000898:Antibiosis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial; D016680:Genome, Bacterial; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism; D016366:Open Reading Frames; D063065:Organophosphonates / Q000378:metabolism; D010453:Peptide Synthases / Q000378:metabolism; D061065:Polyketides / Q000378:metabolism; D012705:Serratia / Q000187:drug effects; D013729:Terpenes / Q000378:metabolism; D014415:Tunicamycin / Q000235:genetics / Q000378:metabolism
chemical_list: D000599:Amino Acids, Diamino; D001426:Bacterial Proteins; D063065:Organophosphonates; D061065:Polyketides; D013729:Terpenes; D014415:Tunicamycin; C045628:ectoine; D006836:Hydro-Lyases; C022771:dTDPglucose 4,6-dehydratase; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase
keywords: Alcaligenes; Antimicrobial; Draft genome; Multidrug resistant; Tunicamycin