title: Analysis of Bacterial Diversity and Communities Associated with Tricholoma matsutake Fruiting Bodies by Barcoded Pyrosequencing in Sichuan Province, Southwest China.
abstract: Endophytes play an important role in the growth and development of the host. However, the study of endophytes is mostly focused on plants, and reports on bacteria associated with fungi are relatively rare. We studied the bacteria associated with fruiting bodies of Tricholoma matsutake picked from seven main T. matsutake-producing areas in Sichuan, China, by barcoded pyrosequencing. About 8,272 reads were obtained per sample, representing 40 phyla, 103 classes, and 495 genera of bacteria and archaea, and 361-797 operational taxonomic units were observed at a 97% similarity level. The bacterial community was always both more abundant and more diverse than the archaeal community. UniFrac analysis showed there were some difference of bacterial communities among the samples sites. Three bacterial phyla, Proteobacteria, Bacteroidetes, and Firmicutes, were dominant in all samples. Correlation analysis showed there was a significant correlation between some soil properties and bacterial community associated with T. matsutake. This study demonstrated that the bacteria associated with T. matsutake fruiting bodies were diversified. Among these bacteria, we may find some strains that can promote the growth of T. matsutake.
authors.affiliation: College of Life Science, Sichuan University, Chengdu, Sichuan 610065, P.R. China.|Institute of Biological & Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610066, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610066, P.R. China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of plant protection, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610066, P.R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: Biotechnology & Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650221, P.R. China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Biological & Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610066, P.R. China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Huang
authors.affiliation: College of Life Science, Sichuan University, Chengdu, Sichuan 610065, P.R. China.|Institute of Biological & Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610066, P.R. China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Institute of Biological & Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610066, P.R. China.
authors.forename: Xing
authors.initials: X
authors.lastname: Jin
authors.affiliation: College of Life Science, Sichuan University, Chengdu, Sichuan 610065, P.R. China.|Institute of Biological & Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610066, P.R. China.
authors.forename: Linyong
authors.initials: L
authors.lastname: Zheng
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D002681:China; D058893:DNA Barcoding, Taxonomic; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry* / Q000254:growth & development; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D010802:Phylogeny; D055439:Tricholoma / Q000737:chemistry* / Q000254:growth & development
keywords: Tricholoma matsutake; barcoded pyrosequencing; community structure; soil properties