title: Behavior of Kinesin Driven Quantum Dots Trapped in a Microtubule Loop.
abstract: We report the observation of kinesin driven quantum dots (QDs) trapped in a microtubule loop, allowing the investigation of moving QDs for a long time and an unprecedented long distance. The QD conjugates did not depart from our observational field of view, enabling the tracking of specific conjugates for more than 5 min. The unusually long run length and the periodicity caused by the loop track allow comparing and studying the trajectory of the kinesin driven QDs for more than 2 full laps, i.e., about 70 μm, enabling a statistical analysis of interactions of the same kinesin driven object with the same obstacle. The trajectories were extracted and analyzed from kymographs with a newly developed algorithm. Despite dispersion, several repetitive trajectory patterns can be identified. A method evaluating the similarity is introduced allowing a quantitative comparison between the trajectories. The velocity variations appear strongly correlated to the presence of obstacles. We discuss the reasons making this long continuous travel distances on the loop track possible.
authors.affiliation: WPI Advanced Institute for Materials Research, Tohoku University , 2-1-1 Katahira, Sendai 980-8577, Japan.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Sikora
authors.affiliation: WPI Advanced Institute for Materials Research, Tohoku University , 2-1-1 Katahira, Sendai 980-8577, Japan.
authors.forename: Filippo Federici
authors.initials: FF
authors.lastname: Canova
authors.affiliation: WPI Advanced Institute for Materials Research, Tohoku University , 2-1-1 Katahira, Sendai 980-8577, Japan.
authors.forename: Kyongwan
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University , Sendai 980-8579, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Nakazawa
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University , Sendai 980-8579, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Umetsu
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University , Sendai 980-8579, Japan.
authors.forename: Izumi
authors.initials: I
authors.lastname: Kumagai
authors.affiliation: WPI Advanced Institute for Materials Research, Tohoku University , 2-1-1 Katahira, Sendai 980-8577, Japan.
authors.forename: Tadafumi
authors.initials: T
authors.lastname: Adschiri
authors.affiliation: Department of Biomedical Engineering, Texas A&M University , College Station, Texas 77843-3120, United States.|School of Computational Sciences, Korea Institute for Advanced Study , Seoul 130-722, Korea.|Materials Science and Engineering, Texas A&M University , College Station, Texas 77843-3003, United States.
authors.forename: Wonmuk
authors.initials: W
authors.lastname: Hwang
authors.affiliation: WPI Advanced Institute for Materials Research, Tohoku University , 2-1-1 Katahira, Sendai 980-8577, Japan.|Materials Science and Engineering, Texas A&M University , College Station, Texas 77843-3003, United States.|Department of Physics and Astronomy, Texas A&M University , College Station, Texas 77843-4242, United States.
authors.forename: Winfried
authors.initials: W
authors.lastname: Teizer
mesh_terms: D000818:Animals; D001681:Biological Assay; D000067493:Dynamic Light Scattering; D016547:Kinesins / Q000378:metabolism*; D007734:Kymography; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism*; D010316:Particle Size; D011336:Probability; D045663:Quantum Dots / Q000378:metabolism*; D034421:Sus scrofa; D014741:Video Recording
chemical_list: D016547:Kinesins
keywords: kinesin; microtubules; molecular transport; motor proteins; quantum dots