title: Improved validation of IDP ensembles by one-bond Cα-Hα scalar couplings.
abstract: Intrinsically disordered proteins (IDPs) are best described by ensembles of conformations and a variety of approaches have been developed to determine IDP ensembles. Because of the large number of conformations, however, cross-validation of the determined ensembles by independent experimental data is crucial. The (1)JCαHα coupling constant is particularly suited for cross-validation, because it has a large magnitude and mostly depends on the often less accessible dihedral angle ψ. Here, we reinvestigated the connection between (1)JCαHα values and protein backbone dihedral angles. We show that accurate amino-acid specific random coil values of the (1)JCαHα coupling constant, in combination with a reparameterized empirical Karplus-type equation, allow for reliable cross-validation of molecular ensembles of IDPs.
authors.affiliation: Computational Biomolecular Dynamics Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Vytautas
authors.initials: V
authors.lastname: Gapsys
authors.affiliation: Department for NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, 37077, Göttingen, Germany.
authors.forename: Raghavendran L
authors.initials: RL
authors.lastname: Narayanan
authors.affiliation: Department for NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, 37077, Göttingen, Germany.
authors.forename: ShengQi
authors.initials: S
authors.lastname: Xiang
authors.affiliation: Computational Biomolecular Dynamics Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Bert L
authors.initials: BL
authors.lastname: de Groot
authors.affiliation: Department for NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, 37077, Göttingen, Germany. markus.zweckstetter@dzne.de.|German Center for Neurodegenerative Diseases (DZNE), 37077, Göttingen, Germany. markus.zweckstetter@dzne.de.|Center for Nanoscale Microscopy and Molecular Physiology of the Brain, University Medical Center Göttingen, 37075, Göttingen, Germany. markus.zweckstetter@dzne.de.
authors.forename: Markus
authors.initials: M
authors.lastname: Zweckstetter
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D064267:Intrinsically Disordered Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods
chemical_list: D000596:Amino Acids; D064267:Intrinsically Disordered Proteins
keywords: Ensemble; Intrinsically disordered protein; NMR; Scalar coupling