title: Porous biphasic calcium phosphate ceramics coated with nano-hydroxyapatite and seeded with mesenchymal stem cells for reconstruction of radius segmental defects in rabbits.
abstract: The osteoconduction of porous biphasic calcium phosphate (BCP) ceramics has been widely reported. In a previous study, we demonstrated that applying a nano-hydroxyapatite (nHA) coating enhances the osteoinductive potential of BCP ceramics, making these scaffolds more suitable for bone tissue engineering applications. The aim of the present study was to determine the effects of reconstructing radius defects in rabbits using nHA-coated BCP ceramics seeded with mesenchymal stem cells (MSCs) and to compare the bone regeneration induced by different scaffolds. Radius defects were created in 20 New Zealand rabbits, which were divided into four groups by treatment: porous BCP ceramics (Group A), nHA-coated porous BCP ceramics (Group B), porous BCP ceramics seeded with rabbit MSCs (Group C), and nHA-coated porous BCP ceramics seeded with rabbit MSCs (Group D). After in vitro incubation, the cell/scaffold complexes were implanted into the defects. Twelve weeks after implantation, the specimens were examined macroscopically and histologically. Both the nHA coating and seeding with MSCs enhanced the formation of new bone tissue in the BCP ceramics, though the osteoinductive potential of the scaffolds with MSCs was greater than that of the nHA-coated scaffolds. Notably, the combination of nHA coating and MSCs significantly improved the bone regeneration capability of the BCP ceramics. Thus, MSCs seeded into porous BCP ceramics coated with nHA may be an effective bone substitute to reconstruct bone defects in the clinic.
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha, People's Republic of China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha, People's Republic of China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Spine Surgery, Honghui Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, People's Republic of China.
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Powder Metallurgy, Central South University, Changsha, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Powder Metallurgy, Central South University, Changsha, People's Republic of China.
authors.forename: Kaiyang
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Sports Medicine, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, People's Republic of China. hongbinlu@hotmail.com.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Lu
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry*; D002516:Ceramics; D020099:Coated Materials, Biocompatible; D017886:Durapatite; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008854:Microscopy, Electron; D053758:Nanoparticles; D016062:Porosity; D011817:Rabbits; D011884:Radius / Q000002:abnormalities*; D017550:Spectroscopy, Fourier Transform Infrared; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D017886:Durapatite; C020243:calcium phosphate