title: MicroRNA-139-5p acts as a tumor suppressor by targeting ELTD1 and regulating cell cycle in glioblastoma multiforme.
abstract: MicroRNA-139-5p was identified to be significantly down-regulated in glioblastoma multiform (GBM) by miRNA array. In this report we aimed to clarify its biological function, molecular mechanisms and direct target gene in GBM. Twelve patients with GBM were analyzed for the expression of miR-139-5p by quantitative RT-PCR. miR-139-5p overexpression was established by transfecting miR-139-5p-mimic into U87MG and T98G cells, and its effects on cell proliferation were studied using MTT assay and colony formation assays. We concluded that ectopic expression of miR-139-5p in GBM cell lines significantly suppressed cell proliferation and inducing apoptosis. Bioinformatics coupled with luciferase and western blot assays also revealed that miR-139-5p suppresses glioma cell proliferation by targeting ELTD1 and regulating cell cycle.
authors.affiliation: Department of Diagnostic Imaging, Linyi People's Hospital, Linyi, Shandong 276000, PR China.
authors.forename: Shouping
authors.initials: S
authors.lastname: Dai
authors.affiliation: Department of Neurology, Linyi People's Hospital, Linyi, Shandong 276000, PR China.
authors.forename: Xianjun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, First Affiliated Hospital of Nanjing Medical University, Nanjing, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Radiotherapy, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, PR China. Electronic address: yuandongcao@sina.com.
authors.forename: Yuandong
authors.initials: Y
authors.lastname: Cao
mesh_terms: D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
chemical_list: C428324:ADGRL4 protein, human; C555098:MIRN139 microRNA, human; D035683:MicroRNAs; D009841:Oligonucleotides; D043562:Receptors, G-Protein-Coupled; D008156:Luciferases
keywords: Cell cycle; ELTD1; Glioma; miR-139-5p