title: Identification and BAC construction of Han, the first characterized HCMV clinical strain in China.
abstract: Human cytomegalovirus (HCMV) is the leading infectious cause of birth defects, and may lead to severe or lethal diseases in immunocompromised individuals. Several HCMV strains have been identified and widely applied in research, but no isolate from China has been characterized. In the present study, we isolated, characterized and sequenced the first Chinese HCMV clinical strain Han, and constructed the novel and functional HCMV infectious clone Han-BAC-2311. HCMV Han was isolated from the urine sample of a Chinese infant with multiple developmental disorders. It expresses HCMV specific proteins and contains a representative HCMV genome with minor differences compared to other strains. By homologous recombination using mini-F derived BAC vector pUS-F6, the infectious clone Han-BAC-2311 was constructed containing representative viral genes across the HCMV genome. The insertion site and orientation of BAC sequence were confirmed by restriction enzyme digestion and Southern blotting. The reconstituted recombinant virus HanBAC-2311 expresses typical viral proteins with the same pattern as that of wild-type Han, and also displayed a similar growth kinetics to wild-type Han. The identification of the first clinical HCMV strain in China and the construction of its infectious clone will greatly facilitate the pathogenesis studies and vaccine development in China.
authors.affiliation: State Key Laboratory of Virology, CAS Center for Excellence in Brain Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Virology, CAS Center for Excellence in Brain Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Zhang-Zhou
authors.initials: ZZ
authors.lastname: Shen
authors.affiliation: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, Shenyang, China.
authors.forename: Zhong-Yang
authors.initials: ZY
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Virology, CAS Center for Excellence in Brain Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Virology, CAS Center for Excellence in Brain Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, Shenyang, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Virology, CAS Center for Excellence in Brain Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Simon
authors.initials: S
authors.lastname: Rayner
authors.affiliation: State Key Laboratory of Virology, CAS Center for Excellence in Brain Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Virology, CAS Center for Excellence in Brain Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Guan-Hua
authors.initials: GH
authors.lastname: Qiao
authors.affiliation: State Key Laboratory of Virology, CAS Center for Excellence in Brain Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Hai-Fei
authors.initials: HF
authors.lastname: Jiang
authors.affiliation: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, Shenyang, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark, New Jersey, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems and State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, CAS Center for Excellence in Brain Science, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Fu-Qiang
authors.initials: FQ
authors.lastname: Xu
authors.affiliation: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, Shenyang, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ruan
authors.affiliation: State Key Laboratory of Virology, CAS Center for Excellence in Brain Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Min-Hua
authors.initials: MH
authors.lastname: Luo
mesh_terms: D044466:Asian People; D002681:China; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000151:congenital / Q000821:virology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D017422:Sequence Analysis, DNA; D014556:Urine / Q000821:virology; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: D014764:Viral Proteins
keywords: Chinese clinical strain; bacterial artificial chromosome; human cytomegalovirus; infectious clone; isolation