title: Limitations on the detection rate of high-risk HPV by hybrid capture 2 methodology in high grade intraepithelial (HSIL) or atypical squamous cells-cannot exclude HSIL (ASC-H) cytological lesions with proved CIN2.
abstract: Recent literature data suggest that the high-risk human papillomaviruses (HR-HPVs) testing with several molecular techniques could be an alternative to cytology in the detection of cervical intraepithelial neoplasias of grade 2 or worse (CIN2+). However, any molecular techniques have its own limits and may give false negative results which must be clearly known before undertaking a primary HPV screening. This study aims to evaluate the performance of the high-risk HPV hybrid capture II detection kit (HCII) which is considered as a "gold standard technique" in a series of 100 women having proved both cytological lesions of atypical squamous cells-cannot exclude an HSIL (ASC-H) or high-grade squamous intraepithelial lesion (HSIL) and histological lesions of CIN2+. The clinical sensitivity of HCII in women with a cytological diagnosis of ASC-H/HSIL and a diagnosis of CIN2+ is high but not absolute and estimated at 96% (95,6% and 100% of women with a diagnosis of CIN2/3 or invasive squamous cell carcinoma, resp.). These data although they are infrequent must be clearly referred before to start an HPV primary screening of CIN2+ especially with HCII methodology.
authors.affiliation: Unit of Gynecopathology, Erasme University Hospital-ULB, Brussels, Belgium.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Noël
authors.affiliation: Unit of Gynecopathology, Erasme University Hospital-ULB, Brussels, Belgium.
authors.forename: Philippe
authors.initials: P
authors.lastname: Simon
mesh_terms: D000328:Adult; D000368:Aged; D065309:Atypical Squamous Cells of the Cervix / Q000473:pathology* / Q000821:virology*; D003584:Cytological Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008875:Middle Aged; D060787:Neoplasm Grading; D027383:Papillomaviridae / Q000302:isolation & purification*; D012307:Risk Factors; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000473:pathology* / Q000821:virology