title: Silica nanoparticles inhibit brown adipocyte differentiation via regulation of p38 phosphorylation.
abstract: Nanoparticles are of great interest due to their wide variety of biomedical and bioengineering applications. However, they affect cellular differentiation and/or intracellular signaling when applied and exposed to target organisms or cells. The brown adipocyte is a cell type important in energy homeostasis and thus closely related to obesity. In this study, we assessed the effects of silica nanoparticles (SNPs) on brown adipocyte differentiation. The results clearly showed that brown adipocyte differentiation was significantly repressed by exposure to SNPs. The brown adipocyte-specific genes as well as mitochondrial content were also markedly reduced. Additionally, SNPs led to suppressed p38 phosphorylation during brown adipocyte differentiation. These effects depend on the size of SNPs. Taken together, these results lead us to suggest that SNP has anti-brown adipogenic effect in a size-dependent manner via regulation of p38 phosphorylation.
authors.affiliation: Functional Genomics Research Center, KRIBB, Daejeon 305-806, Korea.
authors.forename: Min Jeong
authors.initials: MJ
authors.lastname: Son
authors.forename: Won Kon
authors.initials: WK
authors.lastname: Kim
authors.forename: Minjeong
authors.initials: M
authors.lastname: Kwak
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Oh
authors.forename: Won Seok
authors.initials: WS
authors.lastname: Chang
authors.forename: Jeong-Ki
authors.initials: JK
authors.lastname: Min
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Lee
authors.forename: Nam Woong
authors.initials: NW
authors.lastname: Song
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Bae
mesh_terms: D041721:3T3-L1 Cells; D052437:Adipocytes, Brown / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism; D051379:Mice; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010316:Particle Size; D010766:Phosphorylation / Q000187:drug effects; D012822:Silicon Dioxide / Q000737:chemistry*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000071256:Uncoupling Protein 1; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D007473:Ion Channels; D024101:Mitochondrial Proteins; D047495:PPAR gamma; C521133:Prdm16 protein, mouse; D014157:Transcription Factors; D000071256:Uncoupling Protein 1; D012822:Silicon Dioxide; D048051:p38 Mitogen-Activated Protein Kinases