title: [Impact of mesenchymal stem cells transplantation on myocardial myocardin-related transcription factor-A and bcl-2 expression in rats with experimental myocardial infarction].
abstract: OBJECTIVE
  To observe the impact of mesenchymal stem cells (BMSCs) transplantation on myocardial myocardin-related transcription factor-A (MRTF-A) and bcl-2 expression in rats with experimental myocardial infarction (MI).
  
  
  METHODS
  Thirty rats were randomly divided into sham, MI and MI + BMSCs (1 × 10(6) injected into 4 infarct points immediately post coronary artery ligation) groups (n = 10 each).One week later, TUNEL was used to detect cardiomyocyte apoptosis, the myocardial expression of MRTF-A and bcl-2 was detected by laser scanning confocal microscope and Western blot. In vitro plasmid of MRTF-A and co-transfection with plasmids of MRTF-A and bcl-2 or mutated bcl-2 transfection into cardiomyocyte was applied to evaluate the relationship between MRTF-A and bcl-2.
  
  
  RESULTS
  The number of apoptotic cardiomyocytes in the sham group, MI group and MI + BMSCs group were (4.05 ± 1.56)%, (62.38 ± 8.41)% and (22.36 ± 6.17)%, respectively (P < 0.05). The protein expression of MRTF-A and bcl-2 in the MI group were significantly lower than those in sham group, while significantly upregulated in MI + BMSCs group (P < 0.05 vs. MI). In cultured neonatal rat cardiomyocyte, the expression of bcl-2 protein was significantly upregulated after transfection with MRTF-A plasmid, and bcl-2-luciferase activity significantly increased after co-transfection with plasmids of MRTF-A and bcl-2-luciferase, however, the positive regulatory effect of MRTF-A was abolished after transfection with mutated bcl-2.
  
  
  CONCLUSIONS
  Mesenchymal stem cells transplantation can effectively reduce cardiomyocyte apoptosis in this rat MI model, and upregulate the expression of MRTF-A. Consequent up-regulated bcl-2 expression might be involved in the beneficial effects of BMSCs transplantation in this model.
authors.affiliation: Department of Cardiology, Jiande Branch, Second Affiliated Hospital, Zhejiang University School of Medicine, Jiande 311600, China; Email: hzzhongze@163.com.
authors.forename: Ze
authors.initials: Z
authors.lastname: Zhong
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Hu
authors.forename: Yong
authors.initials: Y
authors.lastname: Sun
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.forename: Xindong
authors.initials: X
authors.lastname: Wu
authors.forename: Peng
authors.initials: P
authors.lastname: Xiang
authors.forename: Xiuying
authors.initials: X
authors.lastname: Luo
mesh_terms: D000818:Animals; D017209:Apoptosis; D006321:Heart; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D009203:Myocardial Infarction; D009206:Myocardium; D032383:Myocytes, Cardiac; D009687:Nuclear Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051381:Rats; D017207:Rats, Sprague-Dawley; D015534:Trans-Activators; D014157:Transcription Factors; D014162:Transfection; D000099251:Myocardin
chemical_list: D009687:Nuclear Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D015534:Trans-Activators; D014157:Transcription Factors; D000099251:Myocardin; C000594702:Mrtfa protein, rat