title: A pilot study of antioxidant potential of endophytic fungi from some Sudanese medicinal plants.
abstract: OBJECTIVE
  To evaluate the total phenolic content and total antioxidant capacity of ethyl acetate extracts of 21 endophytic fungi isolated from five Sudanese medicinal plants: Calotropis procera, Catharanthus roseus, Euphorbia prostrate, Vernonia amygdalina and Trigonella foenum-graecum.
  
  
  METHODS
  Crude extracts of endophytic fungi and their host plants were tested by classical Folin-Ciocalteu colorimetric method to determine the total phenolic content, also total antioxidant capacity was estimated using 1,1-diphenyl-2-picrylhydrazyl free radical scavenging in vitro method.
  
  
  RESULTS
  Among the endophytes, endophytic fungus Aspergillus sp. from Trigonella foenum-graecum seeds demonstrated the highest both total phenolic content in term of gallic acid equivalent [(89.9 ± 7.1) mg GAE/g] and antioxidant activity for 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay [IC50: (18.0 ± 0.1) μg/mL]. A high positive linear correlation (R(2) = 0.999 1) was found between total antioxidant capacity and total phenolic content of endophytic fungi isolated from Vernonia amygdalina.
  
  
  CONCLUSIONS
  The present study revealed that some endophytic fungi from the five Sudanese medicinal plants could be a potential source of novel natural antioxidant compounds.
authors.affiliation: Université de Lorraine, SRSMC, UMR 7565, BP 70239, F-54506 Vandoeuvre-lès-Nancy, France; CNRS, SRSMC, UMR 7565, BP 70239, F-54506 Vandoeuvre-lès-Nancy, France; Botany Department, Faculty of Science, University of Khartoum, P.O. Box 321, Khartoum, Sudan.
authors.forename: Afra
authors.initials: A
authors.lastname: Khiralla
authors.affiliation: Botany Department, Faculty of Science, University of Khartoum, P.O. Box 321, Khartoum, Sudan.
authors.forename: Ietidal
authors.initials: I
authors.lastname: Mohamed
authors.affiliation: Plant Advanced Technologies PAT, C/o ENSAIA - INPL, France.
authors.forename: Justinne
authors.initials: J
authors.lastname: Thomas
authors.affiliation: Plant Advanced Technologies PAT, C/o ENSAIA - INPL, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Mignard
authors.affiliation: Université de Lorraine, SRSMC, UMR 7565, BP 70239, F-54506 Vandoeuvre-lès-Nancy, France; CNRS, SRSMC, UMR 7565, BP 70239, F-54506 Vandoeuvre-lès-Nancy, France.
authors.forename: Rosella
authors.initials: R
authors.lastname: Spina
authors.affiliation: Botany Department, Faculty of Science, University of Khartoum, P.O. Box 321, Khartoum, Sudan.
authors.forename: Sakina
authors.initials: S
authors.lastname: Yagi
authors.affiliation: Université de Lorraine, SRSMC, UMR 7565, BP 70239, F-54506 Vandoeuvre-lès-Nancy, France; CNRS, SRSMC, UMR 7565, BP 70239, F-54506 Vandoeuvre-lès-Nancy, France. Electronic address: dominique.mattar@univ-lorraine.fr.
authors.forename: Dominique
authors.initials: D
authors.lastname: Laurain-Mattar
keywords: Antioxidant activity; Endophytic fungi; Sudanese medicinal plants; Total phenolic content