title: Cervical histology after routine ThinPrep or SurePath liquid-based cytology and computer-assisted reading in Denmark.
abstract: BACKGROUND
  We compared the sensitivity and specificity of liquid-based cytology (LBC) and computer-assisted reading for SurePath/FocalPoint and ThinPrep with those of manually read conventional cytology in routine cervical screening in four Danish laboratories.
  
  
  METHODS
  Using data from five nationwide registers, technological phases were identified by slide preparation, reading technique, and triage of borderline cytology. Trends in the detection of cervical intraepithelial neoplasia (CIN) were an indicator of the technology's relative sensitivity, and trends in false-positive tests an indicator of relative specificity.
  
  
  RESULTS
  At 23-29 years, SurePath/FocalPoint statistically significantly increased the detection of ⩾CIN3 by 85% compared with manually read conventional cytology. The 11% increase with ThinPrep was not significant. At 30-44 years, the increase with SurePath/FocalPoint was 58%; the 16% increase with ThinPrep was not significant. At 45-59 years, both technologies led to nonsignificant decreases in the detection. SurePath/FocalPoint doubled the frequency of false-positive tests at any age. With ThinPrep, these proportions remained the same at 23-29 years, but decreased by two-thirds at 45-59 years. In a fourth laboratory with continuous use of manually read conventional cytology, no such trends were seen.
  
  
  CONCLUSIONS
  The sensitivity and specificity of modern LBC and computer-assisted reading technologies may be brand- and age-dependent.
authors.affiliation: Department of Public Health, University of Copenhagen, Øster Farimagsgade 5, Copenhagen 1014, Denmark.
authors.forename: Matejka
authors.initials: M
authors.lastname: Rebolj
authors.affiliation: Department of Public Health, University of Copenhagen, Øster Farimagsgade 5, Copenhagen 1014, Denmark.
authors.forename: Johanne
authors.initials: J
authors.lastname: Rask
authors.affiliation: Department of Public Health, Erasmus MC, 's-Gravendijkwal 230, Rotterdam 3015, the Netherlands.
authors.forename: Marjolein
authors.initials: M
authors.lastname: van Ballegooijen
authors.affiliation: Department of Gynecology and Obstetrics, Copenhagen University Hospital, Kettegård Alle 30, Hvidovre 2650, Denmark.
authors.forename: Benny
authors.initials: B
authors.lastname: Kirschner
authors.affiliation: Department of Public Health, Erasmus MC, 's-Gravendijkwal 230, Rotterdam 3015, the Netherlands.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Rozemeijer
authors.affiliation: Department of Pathology, Copenhagen University Hospital, Kettegård Alle 30, Hvidovre 2650, Denmark.|Clinical Research Centre, Copenhagen University Hospital, Kettegård Alle 30, Hvidovre 2650, Denmark.
authors.forename: Jesper
authors.initials: J
authors.lastname: Bonde
authors.affiliation: Department of Pathology, Copenhagen University Hospital, Kettegård Alle 30, Hvidovre 2650, Denmark.
authors.forename: Carsten
authors.initials: C
authors.lastname: Rygaard
authors.affiliation: Department of Public Health, University of Copenhagen, Øster Farimagsgade 5, Copenhagen 1014, Denmark.
authors.forename: Elsebeth
authors.initials: E
authors.lastname: Lynge
mesh_terms: D000328:Adult; D002584:Cervix Uteri / Q000473:pathology*; D003581:Cytodiagnosis / Q000379:methods*; D003718:Denmark; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D008875:Middle Aged; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D014626:Vaginal Smears / Q000379:methods; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000473:pathology*