title: Investigating the adduct formation of organic mercury species with carbonic anhydrase and hemoglobin from human red blood cell hemolysate by means of LC/ESI-TOF-MS and LC/ICP-MS.
abstract: The interaction of mercury species with human erythrocytes is studied to investigate possible high molecular binding partners for mercury species. Human blood hemolysate was spiked with methylmercury and investigated by means of liquid chromatography (LC) coupled to electrospray ionization time of flight mass spectrometry (ESI-ToF-MS) and inductively coupled plasma mass spectrometry (ICP-MS). Beside adduct formation of mercury species with hemoglobin, the main compound of the erythrocytes, mercury binding to the enzyme carbonic anhydrase was revealed. Due to an enzymatic digest of the protein-mercury adduct, the binding site at the free thiol group of the protein was identified. These results indicate that carbonic anhydrase might play a role in mercury toxicity.
authors.affiliation: Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstraße 28/30, 48149 Münster, Germany. uk@uni-muenster.de.
authors.forename: Jens
authors.initials: J
authors.lastname: Hogeback
authors.affiliation: Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstraße 28/30, 48149 Münster, Germany. uk@uni-muenster.de.
authors.forename: Miriam
authors.initials: M
authors.lastname: Schwarzer
authors.affiliation: Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstraße 28/30, 48149 Münster, Germany. uk@uni-muenster.de.
authors.forename: Christoph A
authors.initials: CA
authors.lastname: Wehe
authors.affiliation: Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstraße 28/30, 48149 Münster, Germany. uk@uni-muenster.de and European Virtual Institute for Speciation Analysis (EVISA), Mendelstr. 11, 48149 Münster, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Sperling
authors.affiliation: Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstraße 28/30, 48149 Münster, Germany. uk@uni-muenster.de.
authors.forename: Uwe
authors.initials: U
authors.lastname: Karst
mesh_terms: D000595:Amino Acid Sequence; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D004912:Erythrocytes / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D008297:Male; D008767:Methylmercury Compounds / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D006454:Hemoglobins; D008767:Methylmercury Compounds; D010455:Peptides; D002256:Carbonic Anhydrases