title: Reference ranges of hematology and lymphocyte subsets in healthy Korean native cattle (Hanwoo) and Holstein dairy cattle.
abstract: There are no accurate reference ranges for hematology parameters and lymphocyte subsets in Korean native beef cattle (Hanwoo). This study was performed to establish reliable reference ranges of hematology and lymphocyte subsets using a large number of Hanwoo cattle (n = 350) and to compare differences between Hanwoo and Holstein dairy cattle (n = 334). Additionally, age-related changes in lymphocyte subsets were studied. Bovine leukocyte subpopulation analysis was performed using mono or dual color flow cytometry. The leukocyte subpopulations investigated in healthy cattle included: CD2(+) cells, sIgM(+) cells, MHC class II(+) cells, CD3(+) CD4(+) cells, CD3(+) CD8(+) cells, and WC1(+) cells. Although Hanwoo and Holstein cattle are the same species, results showed several differences in hematology and lymphocyte subsets between Hanwoo and Holstein cattle. This study is the first report to establish reference ranges of hematology and lymphocyte subsets in adult Hanwoo cattle.
authors.affiliation: Department of Veterinary Infectious Disease, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Yun-Mi
authors.initials: YM
authors.lastname: Kim
authors.affiliation: Department of Veterinary Infectious Disease, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Jin-A
authors.initials: JA
authors.lastname: Lee
authors.affiliation: Center for Pulmonary and Infectious Disease Control, University of Texas Health Science Center at Tyler, Tyler, TX, USA.
authors.forename: Bock-Gie
authors.initials: BG
authors.lastname: Jung
authors.affiliation: Department of Veterinary Infectious Disease, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Tae-Hoon
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Department of Veterinary Infectious Disease, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Bong-Joo
authors.initials: BJ
authors.lastname: Lee
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Guk-Hyun
authors.initials: GH
authors.lastname: Suh
mesh_terms: D000375:Aging / Q000097:blood / Q000276:immunology; D000818:Animals; D001772:Blood Cell Count; D002417:Cattle / Q000097:blood* / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006400:Hematocrit; D006403:Hematologic Tests; D006454:Hemoglobins; D016131:Lymphocyte Subsets; D012016:Reference Values
chemical_list: D006454:Hemoglobins
keywords: Hanwoo cattle; Holstein cattle; hematology; lymphocyte subsets; reference values