title: Intracellular localization and dynamics of Hypericin loaded PLLA nanocarriers by image correlation spectroscopy.
abstract: The study of cell-nanoparticle interactions is an important aspect for understanding drug delivery using nanocarriers. In this regard, advances in fluorescence based microscopy are useful for the investigation of temporal and spatial behavior of nanoparticles (NPs) within the intracellular environment. In this work, we focus on the delivery of the naturally-occurring hydrophobic photosensitizer Hypericin in human lung carcinoma A549 cells by using biodegradable poly L-lactic acid NPs. For the first time, Hypericin containing NPs are prepared by combining the miniemulsion technique with the solvent evaporation method. This approach yields an efficient loading of the NPs with Hypericin and allows for additional cargo molecules. To monitor the release of Hypercin from the NPs, an additional fluorescent lipophilic dye Coumarin-6 is incorporated in the NPs. Temporal and spatiotemporal image correlation spectroscopy is used to determine the fate of the NPs carrying the potential cargo. Both directed and non-directed motions are detected. By using image cross-correlation spectroscopy and specific fluorescent labeling of endosomes, lysosomes and mitochondria, the dynamics of the cargo loaded NPs in association with the organelles is studied.
authors.affiliation: Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium.
authors.forename: Rozhin
authors.initials: R
authors.lastname: Penjweini
authors.affiliation: Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium; Flemish Institute for Technological Research, Environmental Risk and Health Unit, Mol, Belgium.
authors.forename: Sarah
authors.initials: S
authors.lastname: Deville
authors.affiliation: Institute for Materials Research, IMO-IMOMEC, Hasselt University, Diepenbeek, Belgium.
authors.forename: Lien
authors.initials: L
authors.lastname: D'Olieslaeger
authors.affiliation: Institute for Materials Research, IMO-IMOMEC, Hasselt University, Diepenbeek, Belgium.
authors.forename: Mandy
authors.initials: M
authors.lastname: Berden
authors.affiliation: Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium. Electronic address: marcel.ameloot@uhasselt.be.
authors.forename: Marcel
authors.initials: M
authors.lastname: Ameloot
authors.affiliation: Institute for Materials Research, IMO-IMOMEC, Hasselt University, Diepenbeek, Belgium. Electronic address: anitha.ethirajan@uhasselt.be.
authors.forename: Anitha
authors.initials: A
authors.lastname: Ethirajan
mesh_terms: D000873:Anthracenes; D001692:Biological Transport; D045744:Cell Line, Tumor; D003374:Coumarins / Q000008:administration & dosage* / Q000737:chemistry; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010569:Perylene / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D013057:Spectrum Analysis; D013844:Thiazoles / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000873:Anthracenes; D003374:Coumarins; D004337:Drug Carriers; D005456:Fluorescent Dyes; D011091:Polyesters; D011108:Polymers; D013844:Thiazoles; C517282:coumarin 6; D019344:Lactic Acid; C033616:poly(lactide); D010569:Perylene; C004965:hypericin
keywords: Hypericin; Miniemulsion; PLLA nanoparticles; STICCS; STICS; TICS