title: Comprehensive interactome of Otx2 in the adult mouse neural retina.
abstract: The Otx2 homeodomain transcription factor exerts multiple functions in specific developmental contexts, probably through the regulation of different sets of genes. Protein partners of Otx2 have been shown to modulate its activity. Therefore, the Otx2 interactome may play a key role in selecting a precise target-gene repertoire, hence determining its function in a specific tissue. To address the nature of Otx2 interactome, we generated a new recombinant Otx2(CTAP-tag) mouse line, designed for protein complexes purification. We validated this mouse line by establishing the Otx2 interactome in the adult neural retina. In this tissue, Otx2 is thought to have overlapping function with its paralog Crx. Our analysis revealed that, in contrary to Crx, Otx2 did not develop interactions with proteins that are known to regulate phototransduction genes but showed specific partnership with factors associated with retinal development. The relationship between Otx2 and Crx in the neural retina should therefore be considered as complementarity rather than redundancy. Furthermore, study of the Otx2 interactome revealed strong associations with RNA processing and translation machineries, suggesting unexpected roles for Otx2 in the regulation of selected target genes all along the transcription/translation pathway. The Otx2(CTAP-tag) line, therefore, appears suitable for a systematic approach to Otx2 protein-protein interactions. genesis 53:685-694, 2015. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Institut De Biologie Valrose, University of Nice Sophia Antipolis, UFR Sciences, CNRS, UMR7277, Inserm, U1091, Nice, F-06108, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Fant
authors.affiliation: Institut De Biologie Valrose, University of Nice Sophia Antipolis, UFR Sciences, CNRS, UMR7277, Inserm, U1091, Nice, F-06108, France.
authors.forename: Alexander
authors.initials: A
authors.lastname: Samuel
authors.affiliation: Centre De Recherche En Cancérologie De Marseille, INSERM U1068/Institut Paoli-Calmettes, 13273 Marseille CEDEX 9, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Audebert
authors.affiliation: AniRA-PBES, SFR BioSciences Gerland, UMS3444/US8, ENS De Lyon, Lyon, 69007, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Couzon
authors.affiliation: Institut De Biologie Valrose, University of Nice Sophia Antipolis, UFR Sciences, CNRS, UMR7277, Inserm, U1091, Nice, F-06108, France.
authors.forename: Salsabiel
authors.initials: S
authors.lastname: El Nagar
authors.affiliation: Institut De Biologie Valrose, University of Nice Sophia Antipolis, UFR Sciences, CNRS, UMR7277, Inserm, U1091, Nice, F-06108, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Billon
authors.affiliation: Institut De Biologie Valrose, University of Nice Sophia Antipolis, UFR Sciences, CNRS, UMR7277, Inserm, U1091, Nice, F-06108, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lamonerie
mesh_terms: D000818:Animals; D018398:Homeodomain Proteins / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D051857:Otx Transcription Factors / Q000378:metabolism*; D011485:Protein Binding; D012160:Retina / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism
chemical_list: D018398:Homeodomain Proteins; D051857:Otx Transcription Factors; D015534:Trans-Activators; C109288:cone rod homeobox protein
keywords: Otx2; TAP-tag; interactome; retina