title: Optimization of the steam explosion and enzymatic hydrolysis for sugars production from oak woods.
abstract: Fermentable sugars production from three kind of steam-exploded oak wood was optimized by Response Surface Methodology (RSM), using the severity factor (R0), the pretreated total solids (TS%) and the enzyme loading (EL%) as variables of a central composite design. A total of 17 experiments for each biomass were carried out. The optimal conditions established with RSM were: severity, 4.46 for holm, 4.03 for turkey oak and 3.92 for downey oak; total solids, 5.4% for holm, 5.0% for turkey oak and 12.7% for downey oak; and enzyme concentration, 9.6% for holm, 15.0% for turkey oak and 15.0% for downey oak. Under these conditions, the model predicted an overall sugar yield of 67.1% for holm, 79.9% for turkey oak and 68.4% for downey oak. The results of the confirmation experiments under optimal conditions agreed well with model predictions. Oak wood species may be a good feedstock for the production of reducing sugars.
authors.affiliation: Biomass Research Centre, Department of Engineering, Via G. Duranti 67, 06125 Perugia, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Cotana
authors.affiliation: Biomass Research Centre, Department of Engineering, Via G. Duranti 67, 06125 Perugia, Italy. Electronic address: cinzia.buratti@unipg.it.
authors.forename: C
authors.initials: C
authors.lastname: Buratti
authors.affiliation: Biomass Research Centre, Department of Engineering, Via G. Duranti 67, 06125 Perugia, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Barbanera
authors.affiliation: Biomass Research Centre, Department of Engineering, Via G. Duranti 67, 06125 Perugia, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Lascaro
mesh_terms: D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000096:biosynthesis*; D044602:Cellulases / Q000737:chemistry / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D029963:Quercus / Q000737:chemistry* / Q000378:metabolism; D013227:Steam; D014934:Wood / Q000737:chemistry* / Q000378:metabolism
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D013227:Steam; D000431:Ethanol; D044602:Cellulases; D005947:Glucose
keywords: Enzymatic hydrolysis; Ethanol; Oak wood; Response Surface Methodology; Steam explosion