title: Treatment of CIA Mice with FGF21 Down-regulates TH17-IL-17 Axis.
abstract: Recently, FGF21 was reported to play an important role in anti-inflammation. The aim of the study is to explore the mechanism for FGF21 alleviating inflammation of CIA. CIA mice were injected with FGF21 once a day for 28 days after first booster immunization. The results showed that FGF21 alleviates arthritis severity and decreases serum anti-CII antibodies levels in CIA mice. Compared with CIA model, the number of the splenic TH17 cells was significantly decreased in FGF21-treated mice. FGF21 treatment reduced the mRNA expression of IL-17, TNF-α, IL-1β, IL-6, IL-8, and MMP3 and increased level of IL-10 in the spleen tissue. The expression of STAT3 and phosphorylated STAT3 was suppressed in FGF21-treated group. The mRNA expression of RORγt and IL-23 also decreased. In conclusion, these findings suggest that the beneficial effects of FGF21 on CIA mice were achieved by down-regulating Th17-IL-17 axis through STAT3/RORγt pathway. Modulating of Th17-mediated inflammatory response may be one of the mechanisms for FGF21 attenuating inflammation in CIA.
authors.affiliation: Harbin University of Commerce, No. 1 Xuehai Street Songbei Distric, 150028, Harbin, Heilongjiang, China. 1456382154@qq.com.|School of Life Science, Northeast Agricultural University, No. 59 Mucai Street Xiangfang Distric, 150030, Harbin, Heilongjiang, China. 1456382154@qq.com.
authors.forename: Si-Ming
authors.initials: SM
authors.lastname: Li
authors.affiliation: School of Life Science, Northeast Agricultural University, No. 59 Mucai Street Xiangfang Distric, 150030, Harbin, Heilongjiang, China.
authors.forename: Yin-Hang
authors.initials: YH
authors.lastname: Yu
authors.affiliation: School of Life Science, Northeast Agricultural University, No. 59 Mucai Street Xiangfang Distric, 150030, Harbin, Heilongjiang, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Life Science, Northeast Agricultural University, No. 59 Mucai Street Xiangfang Distric, 150030, Harbin, Heilongjiang, China.
authors.forename: Wen-Fei
authors.initials: WF
authors.lastname: Wang
authors.affiliation: School of Life Science, Northeast Agricultural University, No. 59 Mucai Street Xiangfang Distric, 150030, Harbin, Heilongjiang, China. deshanli@163.com.
authors.forename: De-Shan
authors.initials: DS
authors.lastname: Li
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy*; D015536:Down-Regulation; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000627:therapeutic use*; D007249:Inflammation / Q000276:immunology; D016753:Interleukin-10 / Q000096:biosynthesis; D020381:Interleukin-17 / Q000096:biosynthesis*; D053583:Interleukin-1beta / Q000096:biosynthesis; D053760:Interleukin-23 Subunit p19 / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis; D051379:Mice; D008811:Mice, Inbred DBA; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000096:biosynthesis; D050796:STAT3 Transcription Factor / Q000096:biosynthesis / Q000378:metabolism*; D013154:Spleen / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: C556062:IL10 protein, mouse; C587672:IL1B protein, mouse; C098680:Il17a protein, mouse; C508841:Il23a protein, mouse; D020381:Interleukin-17; D053583:Interleukin-1beta; D053760:Interleukin-23 Subunit p19; D015850:Interleukin-6; D016209:Interleukin-8; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D014409:Tumor Necrosis Factor-alpha; C414620:fibroblast growth factor 21; D016753:Interleukin-10; D005346:Fibroblast Growth Factors; D019278:Matrix Metalloproteinase 3; C511769:Mmp3 protein, mouse
keywords: CIA mice; IL-17; TH17 cell; fibroblast growth factor 21(FGF21)