title: Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan.
abstract: For the first time the presence of dinophysistoxin-1 (DTX-1) in a culture of Prorocentrum foraminosum was revealed in cells and in the culture medium. The clone was isolated from coastal waters of the Sea of Japan and identified by molecular analyses of SSU and D1/D2 regions of LSU rDNA. The concentration of DTX-1 in cells was 8.4 ± 2.5 pg/cell and, in cell-free media, 27.9 ± 14.7 µg/L. The toxin presence was confirmed by HPLC with high-resolution tandem mass-spectrometry.
authors.affiliation: Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, Russia. kameneva.p.a@gmail.com.|Far Eastern Federal University, Suhanova St., 8, Vladivostok 690950, Russia. kameneva.p.a@gmail.com.
authors.forename: Polina A
authors.initials: PA
authors.lastname: Kameneva
authors.affiliation: Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, Russia. xengen88@gmail.com.|Far Eastern Federal University, Suhanova St., 8, Vladivostok 690950, Russia. xengen88@gmail.com.
authors.forename: Kseniya V
authors.initials: KV
authors.lastname: Efimova
authors.affiliation: Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, Russia. vgrybin@yahoo.com.
authors.forename: Viacheslav G
authors.initials: VG
authors.lastname: Rybin
authors.affiliation: Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, Russia. torlova06@mail.ru.
authors.forename: Tatiana Y
authors.initials: TY
authors.lastname: Orlova
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002999:Clone Cells; D003470:Culture Media / Q000737:chemistry; D004141:Dinoflagellida / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D057097:Harmful Algal Bloom; D013058:Mass Spectrometry; D009792:Oceans and Seas; D019319:Okadaic Acid / Q000031:analogs & derivatives; D011714:Pyrans / Q000302:isolation & purification*; D012426:Russia; D012623:Seawater / Q000032:analysis / Q000469:parasitology; D013045:Species Specificity
chemical_list: D003470:Culture Media; D011714:Pyrans; D019319:Okadaic Acid; C051904:dinophysistoxin 1
keywords: DTX-1; HPLC-HRMS; Prorocentrum foraminosum; Russia; molecular identification