title: Biomimetic sensor based on molecularly imprinted polymer with nitroreductase-like activity for metronidazole detection.
abstract: The utility of molecularly imprinted polymer (MIP) as electrochemical sensor often suffers from its limited catalytic efficiency. Here, we proposed an alternative approach by combining the concept of MIP with the use of mimetic enzyme. A metronidazole imprinted polymer with nitroreductase-like activity was successfully achieved via an electrochemical method, where melamine served two purposes: functional monomer of MIP and component of mimetic enzyme. During the imprinting process, the redox-active center, which is responsible for catalysis, was introduced into the imprinted cavities. Accordingly, the imprinted polymer, having both catalysis centers and recognition sites, exhibited enhanced electrocatalytic activity and selectivity. The sensing performances of this metronidazole imprinted biomimetic sensor were evaluated in detail. Results revealed that the response to metronidazole was linear in the concentration range of 0.5-1000 μM, and the detection limit was 0.12 μM (S/N=3). In addition, we applied the proposed sensor to detect metronidazole in an injection solution and the results implied its feasibility for practical application.
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Gu
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Yan
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Yaru
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Weilu
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China. Electronic address: zzq@jlu.edu.cn.
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Breast Surgery, First Hospital, Jilin University, Changchun 130012, China. Electronic address: yangming1967@163.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
mesh_terms: D000327:Adsorption; D040761:Biomimetic Materials / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004784:Environmental Monitoring / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008795:Metronidazole / Q000032:analysis*; D054802:Molecular Imprinting / Q000379:methods; D009601:Nitroreductases / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D011108:Polymers; D014874:Water Pollutants, Chemical; D008795:Metronidazole; D009601:Nitroreductases
keywords: Biomimetic sensor; Electrochemistry; Metronidazole; Molecularly imprinted polymer; Nitroreductase