title: Proteomics Analysis Reveals Distinct Corona Composition on Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages.
abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as promising contrast agents for magnetic resonance imaging. The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied. Here, we determined the composition of the plasma protein corona on silica-coated versus dextran-coated SPIONs using mass spectrometry-based proteomics approaches. Notably, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed distinct protein corona compositions for the two different SPIONs. Relaxivity of silica-coated SPIONs was modulated by the presence of a protein corona. Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
authors.affiliation: Division of Molecular Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Carmen
authors.initials: C
authors.lastname: Vogt
authors.affiliation: Clinical Proteomics Mass Spectrometry, Department of Oncology-Pathology, Science for Life Laboratory, and Karolinska Institutet, Stockholm, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Pernemalm
authors.affiliation: Division of Molecular Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Pekka
authors.initials: P
authors.lastname: Kohonen
authors.affiliation: NMR and Molecular Imaging Laboratory, Department of General, Organic and Biomedical Chemistry, University of Mons, Mons, Belgium.
authors.forename: Sophie
authors.initials: S
authors.lastname: Laurent
authors.affiliation: Electron Microscopy Core Facility, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Kjell
authors.initials: K
authors.lastname: Hultenby
authors.affiliation: Division of Metals & Health, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Marie
authors.initials: M
authors.lastname: Vahter
authors.affiliation: Clinical Proteomics Mass Spectrometry, Department of Oncology-Pathology, Science for Life Laboratory, and Karolinska Institutet, Stockholm, Sweden.
authors.forename: Janne
authors.initials: J
authors.lastname: Lehtiö
authors.affiliation: Functional Materials Division, School of Information and Communication Technology, Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Muhammet S
authors.initials: MS
authors.lastname: Toprak
authors.affiliation: Division of Molecular Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Bengt
authors.initials: B
authors.lastname: Fadeel
mesh_terms: D000328:Adult; D002470:Cell Survival; D002478:Cells, Cultured; D003287:Contrast Media; D003911:Dextrans / Q000737:chemistry / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology*; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D013058:Mass Spectrometry; D011485:Protein Binding; D000066970:Protein Corona / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties
chemical_list: D003287:Contrast Media; D003911:Dextrans; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D000066970:Protein Corona; C000499:ferric oxide; D012822:Silicon Dioxide; C065507:ferumoxides