title: High-Resolution Crystal Structure of a Silver(I)-RNA Hybrid Duplex Containing Watson-Crick-like C-Silver(I)-C Metallo-Base Pairs.
abstract: Metallo-base pairs have been extensively studied for applications in nucleic acid-based nanodevices and genetic code expansion. Metallo-base pairs composed of natural nucleobases are attractive because nanodevices containing natural metallo-base pairs can be easily prepared from commercially available sources. Previously, we have reported a crystal structure of a DNA duplex containing T-Hg(II)-T base pairs. Herein, we have determined a high-resolution crystal structure of the second natural metallo-base pair between pyrimidine bases C-Ag(I)-C formed in an RNA duplex. One Ag(I) occupies the center between two cytosines and forms a C-Ag(I)-C base pair through N3-Ag(I)-N3 linear coordination. The C-Ag(I)-C base pair formation does not disturb the standard A-form conformation of RNA. Since the C-Ag(I)-C base pair is structurally similar to the canonical Watson-Crick base pairs, it can be a useful building block for structure-based design and fabrication of nucleic acid-based nanodevices.
authors.affiliation: Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554 (Japan). j.kondo@sophia.ac.jp.|Graduate School of Science and Technology, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554 (Japan). j.kondo@sophia.ac.jp.
authors.forename: Jiro
authors.initials: J
authors.lastname: Kondo
authors.affiliation: Graduate School of Science and Technology, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554 (Japan).
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Tada
authors.affiliation: Laboratory of Molecular Transformation, Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aza-Aoba, Aramaki, Aoba-ku, Sendai 980-8578 (Japan).
authors.forename: Takenori
authors.initials: T
authors.lastname: Dairaku
authors.affiliation: Department of Material and Life Chemistry, Faculty of Engineering, Kanagawa University, 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama 221-8686 (Japan).
authors.forename: Hisao
authors.initials: H
authors.lastname: Saneyoshi
authors.affiliation: Department of Material and Life Chemistry, Faculty of Engineering, Kanagawa University, 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama 221-8686 (Japan).
authors.forename: Itaru
authors.initials: I
authors.lastname: Okamoto
authors.affiliation: Laboratory of Molecular Transformation, Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aza-Aoba, Aramaki, Aoba-ku, Sendai 980-8578 (Japan).|Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, 770-8514 Tokushima (Japan).
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Department of Material and Life Chemistry, Faculty of Engineering, Kanagawa University, 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama 221-8686 (Japan).
authors.forename: Akira
authors.initials: A
authors.lastname: Ono
mesh_terms: D020029:Base Pairing; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D011743:Pyrimidines / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*
chemical_list: D011743:Pyrimidines; D012834:Silver; D012313:RNA; C030986:pyrimidine
keywords: RNA; X-ray analysis; crystal structure; metallo-base pairs; silver