title: Analysis of Spine Motility of Newborn Granule Cells in Acute Brain Slices.
abstract: In this protocol, acute brain slices are prepared from mice in which newborn granule cells have been labeled using retroviral vector technology. Using a live-cell imaging stage and confocal microscopy coupled to imaging software, dendritic spines are analyzed.
authors.forename: Ayumu
authors.initials: A
authors.lastname: Tashiro
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhao
authors.forename: Hoonkyo
authors.initials: H
authors.lastname: Suh
authors.forename: Fred H
authors.initials: FH
authors.lastname: Gage
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D049229:Dendritic Spines / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007231:Infant, Newborn; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D009473:Neuronal Plasticity; D009474:Neurons / Q000166:cytology*; D013131:Spine / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods