title: Single nucleotide polymorphisms in immune system genes and their association with clinical symptoms persistence in dengue-infected persons.
abstract: This study was undertaken to determine the prevalence of dengue clinical symptom persistence during 60days of disease follow up, in patients of Espírito Santo state (ES)-Brazil and to evaluate the relation of single nucleotide polymorphisms (SNPs) in FcγRIIa, CD209, VDR, TNF-α, IL-4, IL-6 and IFN-γ genes with symptom persistence. During 2012-2013, 96 blood samples from individuals diagnosed with symptomatic dengue were collected. Clinical symptom persistence in 60days of follow-up was assessed by a clinical and epidemiological questionnaire filled in 4 interviews. SNP genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In two months of monitoring the dengue infection, we observed that symptoms persisted in 38.5% (37/96) of dengue patients at the end of the first month (D30) and in 11.5% (11/96) of dengue patients at the end of the second month (D60). Our results show an association between FcγRIIa, TNF-α and IL-6 gene SNPs and symptom persistence and an association trend with CD209, IL-4 and IFN-γ gene SNPs. Our findings may increase the knowledge on the pathophysiological mechanisms of persistent symptoms of infection with the dengue virus (DENV) and thus help the clinical management of patients.
authors.affiliation: Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 - Campus de Maruípe, 29040-090 Vitória, ES, Brazil. Electronic address: rasdett@yahoo.com.br.
authors.forename: Raquel Spinassé
authors.initials: RS
authors.lastname: Dettogni
authors.affiliation: Departamento de Clínica Médica, Escola Superior de Ciências da Santa Casa de Misericórdia de Vitória, Av. Nossa Senhora da Penha, 2190 - Santa Luíza, 29045-402 Vitória, ES, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Tristão-Sá
authors.affiliation: Departamento de Medicina, Universidade Federal do Rio Grande do Norte, Campus Caicó, RN 59300-000, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Dos Santos
authors.affiliation: Laboratório de Estatística, Departamento de Estatística, Centro de Ciências Exatas, Universidade Federal do Espírito Santo, Av. Fernando Ferrari, S/N, 29060-900 - Goiabeiras, Vitória, ES, Brazil.
authors.forename: Franciane Figueiredo
authors.initials: FF
authors.lastname: da Silva
authors.affiliation: Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 - Campus de Maruípe, 29040-090 Vitória, ES, Brazil.
authors.forename: Iúri Drumond
authors.initials: ID
authors.lastname: Louro
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology; D003715:Dengue / Q000235:genetics* / Q000276:immunology / Q000473:pathology / Q000821:virology; D003716:Dengue Virus / Q000276:immunology / Q000472:pathogenicity; D005260:Female; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D006801:Humans; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics* / Q000276:immunology; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D018167:Receptors, Calcitriol / Q000235:genetics / Q000276:immunology; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology; D017452:Receptors, IgG / Q000235:genetics* / Q000276:immunology; D012720:Severity of Illness Index; D011795:Surveys and Questionnaires; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000276:immunology; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D007371:Interferon-gamma; D015847:Interleukin-4; D015850:Interleukin-6; D037181:Lectins, C-Type; D018167:Receptors, Calcitriol; D011956:Receptors, Cell Surface; D017452:Receptors, IgG; D014409:Tumor Necrosis Factor-alpha; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C080568:Fc gamma receptor IIA; C508598:IL4 protein, human; C508600:IL6 protein, human; C579457:VDR protein, human
keywords: Cytokines; Dengue; Immune system SNPs; Receptors; Symptoms persistent