title: Molecualr Cloning of the capsular antigen F1 of Yersinia pestis in pBAD/gIII plasmid.
abstract: Yersinia pestis which is the causative agent of pneumonic plague and distributed in all continents has led to many deaths during the history. Because of its high mortality rate, it must be diagnosed and treated at the earliest time post infection and therefore, rapid diagnostic tests are required. In the present study, we cloned the coding sequence of F1 capsular antigen of the bacteria in the pBAD/gIII plasmid for later expression and purification of the protein to produce poly and monoclonal antibodies against this antigen, and subsequently to develop rapid and efficient diagnostics tools for Y. pestis infections.
authors.affiliation: Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: A
authors.initials: A
authors.lastname: Jahanian-Najafabadi
authors.affiliation: Tasinim Biotechnology Research Center, AJA University of Medical Sciences, Tehran, I.R. Iran.
authors.forename: M
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Department of Virology, Pasteur Institute of Iran, Tehran, I.R. Iran.
authors.forename: K
authors.initials: K
authors.lastname: Azadmanesh
authors.affiliation: Department of Epidemiology, Pasteur Institute of Iran, Tehran, I.R. Iran.
authors.forename: E
authors.initials: E
authors.lastname: Mostafavi
authors.affiliation: Tasinim Biotechnology Research Center, AJA University of Medical Sciences, Tehran, I.R. Iran.
authors.forename: K
authors.initials: K
authors.lastname: Majidzadeh-A
keywords: Caf1; Capsular antigen F1; Yersinia. pestis; pBAD