title: Shotgun assembly of the mitochondrial genome from Fenneropenaeus penicillatus with phylogenetic consideration.
abstract: The complete mitochondrial genome is of great importance for better understanding of the genome-level characteristics and phylogenetic relationships among related species. In this study, Fenneropenaeus penicillatus mitochondrial genome sequence was determined by next-generation sequencing. The complete genome DNA was 16,040 bp in length and consisted of a typical set of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and a putative control region (CR). The gene arrangement is identical to the pancrustacean pattern. The overall base composition of its mitochondrial genome is estimated to be 34.1% for A, 34.1% for T, 12.5% for G and 19.3% for C with a high A+T content (68.2%). The analysis of the average Ka/Ks in the 13 mitochondrial protein-coding genes of penaeid shrimps indicated a strong purifying selection within this group. The phylogenetic analysis based on mitochondrial sequences and 13 concatenated protein-coding genes showed strong statistic support for the following relationship among the five genera ((Penaeus s.s+Fenneropenaeus)+(Litopenaeus+Farfantepenaeus))+Marsupenaeus. The sequence data of F. penicillatus can provide useful information for the studies on molecular systematics, population structure, stock evaluation and conservation genetics.
authors.affiliation: Key Laboratory of South China Sea Fishery Research Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China. Electronic address: zhangdch@163.com.
authors.forename: Dianchang
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Key Laboratory of South China Sea Fishery Research Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; College of Fisheries and Life, Shanghai Ocean University, Shanghai; 201306, China.
authors.forename: Fahui
authors.initials: F
authors.lastname: Gong
authors.affiliation: Key Laboratory of South China Sea Fishery Research Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; College of Fisheries and Life, Shanghai Ocean University, Shanghai; 201306, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Liu
authors.affiliation: Key Laboratory of South China Sea Fishery Research Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
authors.forename: Huayang
authors.initials: H
authors.lastname: Guo
authors.affiliation: Key Laboratory of South China Sea Fishery Research Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Key Laboratory of South China Sea Fishery Research Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
authors.forename: Kecheng
authors.initials: K
authors.lastname: Zhu
authors.affiliation: Key Laboratory of South China Sea Fishery Research Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China. Electronic address: jiangsg@21cn.com.
authors.forename: Shigui
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology; D054629:Genome, Mitochondrial / Q000235:genetics*; D009690:Nucleic Acid Conformation; D033561:Penaeidae / Q000235:genetics*; D010802:Phylogeny; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics
chemical_list: D011506:Proteins; D012335:RNA, Ribosomal; D004247:DNA; D012343:RNA, Transfer
keywords: Mitochondrial genome; Penaeidae; Phylogenetic relationship; Shotgun assembly; Shrimp