title: Hydrogen peroxide sensing and cytotoxicity activity of Acacia lignin stabilized silver nanoparticles.
abstract: The study is aimed at detection of hydrogen peroxide (H2O2) using Acacia lignin mediated silver nanoparticles (AGNPs). The synthesis of AGNPs was achieved at conditions optimized as, 3 ml of 0.02% lignin and 1mM silver nitrate incubated for 30 min at 80°C and pH 9. Initial screening of AGNPs was performed by measuring the surface plasmon resonance peak at 410-430 nm using UV-vis spectrophotometer. Transmission electron microscopy, atomic force microscopy, X-ray diffraction and particle size analysis confirmed the spherical shaped face centered cubic structure and 10-50 nm size of AGNPs. The infrared spectroscopy study further revealed that the active functional groups present in lignin were responsible for the reduction of silver ions (Ag(+)) to metallic silver (Ag(0)). Lignin stabilized silver nanoparticles showed good sensitivity and a linear response over wide concentrations of H2O2 (10(-1) to 10(-6)M). Further, the in vitrocytotoxicity activity of the lignin mediated AGNPs (5-500 μg/ml) demonstrated toxicity effects in MCF-7 and A375 cell lines. Thus, lignin stabilized silver nanoparticles based optical sensor for H2O2 could be potentially applied in the determination of reactive oxygen species and toxic chemicals which further expands the importance of lignin stabilized silver nanoparticles.
authors.affiliation: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya (Central University), Bilaspur 495009, Chhattisgarh, India.
authors.forename: Keshaw Ram
authors.initials: KR
authors.lastname: Aadil
authors.affiliation: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya (Central University), Bilaspur 495009, Chhattisgarh, India.
authors.forename: Anand
authors.initials: A
authors.lastname: Barapatre
authors.affiliation: Tumor Biology Laboratory, National Institute of Pathology, New Delhi 110029, India.
authors.forename: Avtar Singh
authors.initials: AS
authors.lastname: Meena
authors.affiliation: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya (Central University), Bilaspur 495009, Chhattisgarh, India. Electronic address: harit74@yahoo.co.in.
authors.forename: Harit
authors.initials: H
authors.lastname: Jha
mesh_terms: D000045:Acacia / Q000737:chemistry*; D015374:Biosensing Techniques; D045744:Cell Line, Tumor; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000737:chemistry* / Q000633:toxicity; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D010316:Particle Size; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D012834:Silver; D008031:Lignin; D006861:Hydrogen Peroxide
keywords: Acacia lignin; Hydrogen peroxide sensing; In vitro cytotoxicity; Silver nanoparticles