title: Characterization of antioxidant phenolics in Syringa vulgaris L. flowers and fruits by HPLC-DAD-ESI-MS.
abstract: In this study the polyphenolic composition of lilac flowers and fruits was determined for the first time. For the identification of compounds, accurate molecular masses and formulas, acquired by LC and ESI-TOF-MS and fragmentation pattern given by LC-ESI/MS/MS analyses, were used. Our chromatographic system in conjunction with tandem MS was found to be valuable in the rapid separation and determination of the multiple constituents in methanolic extracts of lilac flowers and fruits. Altogether 34 phenolics, comprising 18 secoiridoids, seven phenylpropanoids, four flavonoids and five low-molecular-weight phenols, were identified. As marker compounds two secoiridoids (oleuropein and nuzhenide), two phenylpropanoids (acteoside and echinacoside) and rutin were quantified by validated methods. As a result of quantitative analysis, it was confirmed that flowers contain significant amounts of phenylpropanoids (acteoside, 2.48%; echinacoside, 0.75%) and oleuropein (0.95%), while in fruits secoiridoid oleuropein (1.09%) and nuzhenide (0.42%) are the major secondary metabolites. The radical scavenging activities of the extracts and the constituents were investigated by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS [2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)] assays. Both extracts show remarkable antioxidant activities. Our results clearly show that lilac flowers and fruits are inexpensive, readily available natural sources of phenolic compounds with pharmacological and cosmetic applications. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Department of Pharmaceutical Chemistry, Semmelweis University; Research Group of Drugs of Abuse and Doping Agents, Hungarian Academy of Sciences, Hőgyes Endre u 9, Budapest, 1092, Hungary.
authors.forename: Gergő
authors.initials: G
authors.lastname: Tóth
authors.affiliation: Department of Pharmacognosy and Phytotherapy, University of Medicine and Pharmacy, 38 Gh. Marinescu, 540139, Targu Mures, Romania.
authors.forename: Csenge
authors.initials: C
authors.lastname: Barabás
authors.affiliation: Department of Pharmacognosy, Semmelweis University, Üllői út 26, Budapest, 1085, Hungary.
authors.forename: Anita
authors.identifier: http://orcid.org/0000-0001-7117-898X
authors.initials: A
authors.lastname: Tóth
authors.affiliation: Department of Pharmacognosy, Semmelweis University, Üllői út 26, Budapest, 1085, Hungary.
authors.forename: Ágnes
authors.initials: Á
authors.lastname: Kéry
authors.affiliation: Department of Pharmacognosy, Semmelweis University, Üllői út 26, Budapest, 1085, Hungary.
authors.forename: Szabolcs
authors.initials: S
authors.lastname: Béni
authors.affiliation: Institute of Biology, Department of Plant Anatomy, L. Eötvös University, Pázmány Péter sétány 1/C, Budapest, 1117, Hungary.
authors.forename: Imre
authors.initials: I
authors.lastname: Boldizsár
authors.affiliation: Department of Pharmacognosy and Phytotherapy, University of Medicine and Pharmacy, 38 Gh. Marinescu, 540139, Targu Mures, Romania.
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Varga
authors.affiliation: Department of Pharmaceutical Chemistry, Semmelweis University; Research Group of Drugs of Abuse and Doping Agents, Hungarian Academy of Sciences, Hőgyes Endre u 9, Budapest, 1092, Hungary.
authors.forename: Béla
authors.initials: B
authors.lastname: Noszál
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D035264:Flowers / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D031662:Syringa / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010636:Phenols
keywords: LC-MS; Syringa vulgaris; acteoside; identification; secoiridoid; validation