title: Restricted antibody response to Bordetella pertussis filamentous hemagglutinin induced by whole-cell and acellular pertussis vaccines.
abstract: BACKGROUND
  Filamentous hemagglutinin (FHA) is a principal virulence factor, an important immunogenic antigen of Bordetella pertussis, and a major component of many acellular pertussis vaccines. In the present study, the human antibody response to different regions of FHA was determined in healthy children and adults vaccinated with either whole-cell or acellular pertussis vaccines.
  
  
  METHODS
  To define the immunodominant regions of FHA, four overlapping recombinant fragments were expressed and produced in Escherichia coli and then purified by His-tagged based affinity chromatography. Two groups comprising healthy preschool children (n = 50) and adults (n = 26) were vaccinated with a single dose of commercial whole-cell and acellular DTaP vaccines, respectively. An antigen-based ELISA was applied to measure serum levels of anti-FHA antibody to both native and recombinant proteins in vaccinated volunteers.
  
  
  RESULTS
  In both groups of vaccinated individuals, the anti-FHA antibody response was mainly directed against epitopes located within a fragment of FHA spanning amino acid residues 1877-2250 of the mature FHA molecule (p < 0.001). No or little antibody was detected against the other recombinant segments of FHA.
  
  
  CONCLUSIONS
  Our results suggest that the human antibody response to FHA is directed to an immunodominant region located within residues 1877-2250 of the FHA molecule. Characterization and epitope mapping of the major components of acellular pertussis vaccine and future modifications in vaccine formulation may improve its efficacy and protectivity.
authors.affiliation: a Department of Immunology , School of Public Health, Tehran University of Medical Sciences , Tehran , Iran .|b Department of Immunology, School of Medicine , Mazandaran University of Medical Sciences , Sari , Iran .
authors.forename: Hossein
authors.initials: H
authors.lastname: Asgarian-Omran
authors.affiliation: a Department of Immunology , School of Public Health, Tehran University of Medical Sciences , Tehran , Iran .
authors.forename: Maryam
authors.initials: M
authors.lastname: Golara
authors.affiliation: a Department of Immunology , School of Public Health, Tehran University of Medical Sciences , Tehran , Iran .
authors.forename: Sara
authors.initials: S
authors.lastname: Abdolmaleki
authors.affiliation: a Department of Immunology , School of Public Health, Tehran University of Medical Sciences , Tehran , Iran .
authors.forename: Shadi Sadat
authors.initials: SS
authors.lastname: Navabi
authors.affiliation: c Department of Agronomy and Plant Breeding, Campus of Agriculture and Natural Resource , Tehran University , Karaj , and.
authors.forename: Hadi
authors.initials: H
authors.lastname: Alipour
authors.affiliation: a Department of Immunology , School of Public Health, Tehran University of Medical Sciences , Tehran , Iran .
authors.forename: Jalal
authors.initials: J
authors.lastname: Khoshnoodi
authors.affiliation: d Monoclonal Antibody Research Center, Avicenna Research Institute , ACECR , Tehran , Iran.
authors.forename: Azam
authors.initials: A
authors.lastname: Hemmati
authors.affiliation: d Monoclonal Antibody Research Center, Avicenna Research Institute , ACECR , Tehran , Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Zarei
authors.affiliation: d Monoclonal Antibody Research Center, Avicenna Research Institute , ACECR , Tehran , Iran.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Jeddi-Tehrani
authors.affiliation: a Department of Immunology , School of Public Health, Tehran University of Medical Sciences , Tehran , Iran .|d Monoclonal Antibody Research Center, Avicenna Research Institute , ACECR , Tehran , Iran.
authors.forename: Fazel
authors.initials: F
authors.lastname: Shokri
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology; D008297:Male; D010567:Pertussis Vaccine / Q000008:administration & dosage* / Q000276:immunology*; D022282:Vaccines, Acellular / Q000008:administration & dosage / Q000276:immunology; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology*
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D018985:Epitopes, B-Lymphocyte; D016056:Immunodominant Epitopes; D010567:Pertussis Vaccine; D022282:Vaccines, Acellular; D015164:Vaccines, Inactivated; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis
keywords: Bordetella pertussis; Filamentous hemagglutinin; acellular pertussis vaccine; whole-cell pertussis vaccine