title: Quantitative Bioimaging to Investigate the Uptake of Mercury Species in Drosophila melanogaster.
abstract: The uptake of mercury species in the model organism Drosophila melanogaster was investigated by elemental bioimaging using laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS). The mercury distribution in Drosophila melanogaster was analyzed for the three species mercury(II) chloride, methylmercury chloride, and thimerosal after intoxication. A respective analytical method was developed and applied to the analysis of the entire Drosophila melanogaster first, before a particular focus was directed to the cerebral areas of larvae and adult flies. For quantification of mercury, matrix-matched standards based on gelatin were prepared. Challenges of spatially dissolved mercury determination, namely, strong evaporation issues of the analytes and an inhomogeneous distribution of mercury in the standards due to interactions with cysteine containing proteins of the gelatin were successfully addressed by complexation with meso-2,3-dimercaptosuccinic acid (DMSA). No mercury was detected in the cerebral region for mercury(II) chloride, whereas both organic species showed the ability to cross the blood-brain barrier. Quantitatively, the mercury level in the brain exceeded the fed concentration indicating mercury enrichment, which was approximately 3 times higher for methylmercury chloride than for thimerosal.
authors.affiliation: University of Münster , Institute of Inorganic and Analytical Chemistry, Corrensstrasse 28/30, 48149 Münster, Germany.|NRW Graduate School of Chemistry, University of Münster , 48149 Münster, Germany.
authors.forename: Ann-Christin
authors.initials: AC
authors.lastname: Niehoff
authors.affiliation: University of Münster , Institute of Inorganic and Analytical Chemistry, Corrensstrasse 28/30, 48149 Münster, Germany.
authors.forename: Oliver Bolle
authors.initials: OB
authors.lastname: Bauer
authors.affiliation: University of Münster , Institute of Inorganic and Analytical Chemistry, Corrensstrasse 28/30, 48149 Münster, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Kröger
authors.affiliation: University of Münster , Institute of Inorganic and Analytical Chemistry, Corrensstrasse 28/30, 48149 Münster, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Fingerhut
authors.affiliation: University Hospital Münster , Institute of Neuropathology, Pottkamp 2, 48149 Münster, Germany.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Schulz
authors.affiliation: NRW Graduate School of Chemistry, University of Münster , 48149 Münster, Germany.|University of Potsdam , Institute of Nutritional Science, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany.
authors.forename: Sören
authors.initials: S
authors.lastname: Meyer
authors.affiliation: University of Münster , Institute of Inorganic and Analytical Chemistry, Corrensstrasse 28/30, 48149 Münster, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Sperling
authors.affiliation: University Hospital Münster , Institute of Neuropathology, Pottkamp 2, 48149 Münster, Germany.
authors.forename: Astrid
authors.initials: A
authors.lastname: Jeibmann
authors.affiliation: University of Potsdam , Institute of Nutritional Science, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany.
authors.forename: Tanja
authors.initials: T
authors.lastname: Schwerdtle
authors.affiliation: University of Münster , Institute of Inorganic and Analytical Chemistry, Corrensstrasse 28/30, 48149 Münster, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Karst
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000737:chemistry / Q000378:metabolism*; D007834:Lasers; D013058:Mass Spectrometry; D008628:Mercury / Q000032:analysis* / Q000378:metabolism*
chemical_list: D008628:Mercury