title: Angiogenic Factors and Doppler Evaluation in Normally Growing Fetuses at Routine Third-Trimester Scan: Prediction of Subsequent Low Birth Weight.
abstract: OBJECTIVE
  To evaluate in normally growing fetuses at routine 32-36 weeks scan the performance of maternal angiogenic factors, Doppler and ultrasound indices in predicting smallness for gestational age (SGA) at birth.
  
  
  METHODS
  A cohort of 1,000 singleton pregnancies with normal estimated fetal weight (EFW, ≥10th centile) at 32-36 weeks scan was included. At inclusion, Doppler indices (mean uterine artery pulsatility index [mUtA-PI], cerebroplacental ratio and normalized umbilical vein blood flow by EFW (ml/min/kg) were evaluated, and blood samples were collected and frozen. Nested in this cohort, maternal circulating placental growth factor (PlGF) and soluble fms-like tyrosine kinase-1 (sFlt-1) were assayed by enzyme-linked immunosorbent assay in all cases with a birth weight <10th centile by customized standards and in an equivalent number of controls (birth weight ≥10th centile).
  
  
  RESULTS
  160 cases were included (80 SGA and 80 controls). EFW (2,128 vs. 2,279 g, p < 0.001), mUtA-PI z-values (-0.25 vs. -0.65, p = 0.034) and sFlt-1/PlGF ratio (11.10 vs. 6.74, p < 0.005) were lower in SGA. The combination of sFlt-1/PlGF ratio and EFW resulted in a 66.3% detection rate for subsequent SGA, with 20% of false-positives. Fetal Doppler indices were not predictive of SGA.
  
  
  CONCLUSIONS
  In normally growing fetuses, maternal angiogenic factors add to ultrasound parameters in predicting subsequent SGA at birth. This supports further research to investigate composite scores in order to improve the definition and identification of fetal growth restriction.
authors.affiliation: BCNatal - Barcelona Center for Maternal-Fetal and Neonatal Medicine (Hospital Clx00ED;nic and Hospital Sant Joan de Deu), IDIBAPS, University of Barcelona, Barcelona, Spain.
authors.forename: Stefania
authors.initials: S
authors.lastname: Triunfo
authors.forename: Miguel
authors.initials: M
authors.lastname: Parra-Saavedra
authors.forename: Victor
authors.initials: V
authors.lastname: Rodriguez-Sureda
authors.forename: Francesca
authors.initials: F
authors.lastname: Crovetto
authors.forename: Carmen
authors.initials: C
authors.lastname: Dominguez
authors.forename: Eduard
authors.initials: E
authors.lastname: Gratacós
authors.forename: Francesc
authors.initials: F
authors.lastname: Figueras
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D015331:Cohort Studies; D005260:Female; D005317:Fetal Growth Retardation / Q000000981:diagnostic imaging*; D006801:Humans; D007230:Infant, Low Birth Weight; D007231:Infant, Newborn; D016015:Logistic Models; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D012372:ROC Curve; D016216:Ultrasonography, Prenatal
chemical_list: D043925:Angiogenesis Inducing Agents; D015415:Biomarkers