title: Application of enzymatic apple pomace hydrolysate to production of 2,3-butanediol by alkaliphilic Bacillus licheniformis NCIMB 8059.
abstract: 2,3-Butanediol (2,3-BD) synthesis by a nonpathogenic bacterium Bacillus licheniformis NCIMB 8059 from enzymatic hydrolysate of depectinized apple pomace and its blend with glucose was studied. In shake flasks, the maximum diol concentration in fed-batch fermentations was 113 g/L (in 163 h, from the hydrolysate, feedings with glucose) while in batch processes it was around 27 g/L (in 32 h, from the hydrolysate and glucose blend). Fed-batch fermentations in the 0.75 and 30 L fermenters yielded 87.71 g/L 2,3-BD in 160 h, and 72.39 g/L 2,3-BD in 94 h, respectively (from the hydrolysate and glucose blend, feedings with glucose). The hydrolysate of apple pomace, which was for the first time used for microbial 2,3-BD production is not only a source of sugars but also essential minerals.
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland.
authors.forename: Aneta M
authors.initials: AM
authors.lastname: Białkowska
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Gromek
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Krysiak
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Sikora
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland. halina.kalinowska@p.lodz.pl.
authors.forename: Halina
authors.initials: H
authors.lastname: Kalinowska
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Jędrzejczak-Krzepkowska
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland.
authors.forename: Celina
authors.initials: C
authors.lastname: Kubik
authors.affiliation: Institute of Biochemistry and Biotechnology, Technical University Braunschweig, Spielmannstrasse 7, 38106, Brunswick, Germany.
authors.forename: Siegmund
authors.initials: S
authors.lastname: Lang
authors.affiliation: Thünen Institute of Wood Research, Leuschnerstrasse 91, 21031, Hamburg, Germany.
authors.forename: Fokko
authors.initials: F
authors.lastname: Schütt
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland.
authors.forename: Marianna
authors.initials: M
authors.lastname: Turkiewicz
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D001407:Bacillus / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D002072:Butylene Glycols / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D027845:Malus / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D002072:Butylene Glycols; C026978:2,3-butylene glycol; D005947:Glucose
keywords: 2,3-Butanediol; Apple pomace hydrolysates; Bacillus licheniformis; Fermentation