title: Cytotoxicity and mutagenicity of sidestream cigarette smoke particulate matter of different particle sizes.
abstract: Sidestream cigarette smoke, the major component of environmental tobacco smoke, is a complex and reactive aerosol. The particulate matter (PM) in sidestream smoke is one of the carriers of chemical constituents. However, particle size-dependent toxicological effects of PM are poorly understood. In this study, we evaluated the relationship between the PM size and in vitro cytotoxicity and mutagenicity of sidestream cigarette smoke. A NanoMoudi-II(™) 125A was used to collect PM samples ranging from 10 nm to 10 μm. The in vitro toxicity of PM was evaluated using a neutral red cytotoxicity assay and Salmonella mutagenicity assay. The results showed that the cytotoxicity and mutagenicity of PM larger than 1 μm was significantly lower than PM sized 10 nm-1 μm. Furthermore, there was a noticeable trend that the smaller the size of the PM of sidestream cigarette smoke, the greater the toxicity. This study suggests that the toxicity of PM in sidestream cigarette smoke is size-dependent.
authors.affiliation: Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou, 450001, China. lixiang79ben@sina.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Tianjin Institute of Health and Environmental Medicine, Tianjin, 300050, China.
authors.forename: Bencheng
authors.initials: B
authors.lastname: Lin
authors.affiliation: Tianjin Institute of Health and Environmental Medicine, Tianjin, 300050, China.
authors.forename: Huashan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou, 450001, China.
authors.forename: Fuwei
authors.initials: F
authors.lastname: Xie
authors.affiliation: Tianjin Institute of Health and Environmental Medicine, Tianjin, 300050, China.
authors.forename: Na
authors.initials: N
authors.lastname: Ta
authors.affiliation: Tianjin Institute of Health and Environmental Medicine, Tianjin, 300050, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tian
authors.affiliation: Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou, 450001, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Liu
authors.affiliation: Tianjin Institute of Health and Environmental Medicine, Tianjin, 300050, China. zhugexi2003@sina.cn.
authors.forename: Zhuge
authors.initials: Z
authors.lastname: Xi
mesh_terms: D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D010316:Particle Size; D052638:Particulate Matter; D014028:Tobacco Smoke Pollution / Q000032:analysis
chemical_list: D009153:Mutagens; D052638:Particulate Matter; D014028:Tobacco Smoke Pollution
keywords: Cytotoxicity; Mutagenicity; Particle size; Sidestream cigarette smoke