title: Spatiotemporally Controlled Reorganization of Signaling Complexes in the Plasma Membrane of Living Cells.
abstract: Triggered immobilization of proteins in the plasma membrane of living cells into functional micropatterns is established by using an adaptor protein, which is comprised of an antiGFP nanobody fused to the HaloTag protein. Efficient in situ reorganization of the type I interferon receptor subunits as well as intact, fully functional signaling complexes in living cells are achieved by this method.
authors.affiliation: Department of BiologyUniversity of Osnabrück, Barbarastr. 11, Osnabrück, 49076, Germany.
authors.forename: Tim
authors.initials: T
authors.lastname: Wedeking
authors.affiliation: Department of BiologyUniversity of Osnabrück, Barbarastr. 11, Osnabrück, 49076, Germany.
authors.forename: Sara
authors.initials: S
authors.lastname: Löchte
authors.affiliation: Department of BiologyUniversity of Osnabrück, Barbarastr. 11, Osnabrück, 49076, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Birkholz
authors.affiliation: Department of BiologyUniversity of Osnabrück, Barbarastr. 11, Osnabrück, 49076, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Wallenstein
authors.affiliation: Institute of Medical Physics and Biophysics, University of Münster, Robert-Koch-Str. 31, Münster, 48149, Germany.|Cells-in-Motion Cluster of Excellence (EXC 1003-CiM), University of Münster, Münster, 48149, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Trahe
authors.affiliation: Institute of Medical Physics and Biophysics, University of Münster, Robert-Koch-Str. 31, Münster, 48149, Germany.|Cells-in-Motion Cluster of Excellence (EXC 1003-CiM), University of Münster, Münster, 48149, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Klingauf
authors.affiliation: Department of BiologyUniversity of Osnabrück, Barbarastr. 11, Osnabrück, 49076, Germany.
authors.forename: Jacob
authors.initials: J
authors.lastname: Piehler
authors.affiliation: Department of BiologyUniversity of Osnabrück, Barbarastr. 11, Osnabrück, 49076, Germany.
authors.forename: Changjiang
authors.initials: C
authors.lastname: You
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D002470:Cell Survival; D006367:HeLa Cells; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D055616:Microtechnology; D011956:Receptors, Cell Surface / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D055767:Immobilized Proteins; D008565:Membrane Proteins; D011956:Receptors, Cell Surface
keywords: cell micropatterning; cytokine signaling; fluorescence imaging; imaging, plasma membranes, type I interferon receptors; microcontact printing