title: Applications of Next-generation Sequencing in Systemic Autoimmune Diseases.
abstract: Systemic autoimmune diseases are a group of heterogeneous disorders caused by both genetic and environmental factors. Although numerous causal genes have been identified by genome-wide association studies (GWAS), these susceptibility genes are correlated to a relatively low disease risk, indicating that environmental factors also play an important role in the pathogenesis of disease. The intestinal microbiome, as the main symbiotic ecosystem between the host and host-associated microorganisms, has been demonstrated to regulate the development of the body's immune system and is likely related to genetic mutations in systemic autoimmune diseases. Next-generation sequencing (NGS) technology, with high-throughput capacity and accuracy, provides a powerful tool to discover genomic mutations, abnormal transcription and intestinal microbiome identification for autoimmune diseases. In this review, we briefly outlined the applications of NGS in systemic autoimmune diseases. This review may provide a reference for future studies in the pathogenesis of systemic autoimmune diseases.
authors.affiliation: Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical Collage, Beijing 100021, China.
authors.forename: Yiyangzi
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical Collage, Beijing 100021, China.
authors.forename: Na
authors.initials: N
authors.lastname: Shi
authors.affiliation: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical Collage, Beijing 100730, China.
authors.forename: Mengtao
authors.initials: M
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.affiliation: Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical Collage, Beijing 100021, China. Electronic address: htniu1@yahoo.com.
authors.forename: Haitao
authors.initials: H
authors.lastname: Niu
mesh_terms: D000818:Animals; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D000069196:Gastrointestinal Microbiome / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology; D051379:Mice; D009103:Multiple Sclerosis / Q000235:genetics* / Q000276:immunology; D009154:Mutation / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D051381:Rats; D017422:Sequence Analysis, DNA; D013167:Spondylitis, Ankylosing / Q000235:genetics* / Q000276:immunology
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Intestinal microbiome; Next-generation sequencing; Susceptibility genes; Systemic autoimmune diseases