title: C57BL/6 and A/J Mice Have Different Inflammatory Response and Liver Lipid Profile in Experimental Alcoholic Liver Disease.
abstract: Alcoholic liver disease (ALD) is an important worldwide public health issue characterized by liver steatosis, inflammation, necrosis, and apoptosis of hepatocytes with eventual development of fibrosis and cirrhosis. Comparison of murine models with different inflammatory responses for ALD is important for an evaluation of the importance of genetic background in the interpretation of ethanol-induced phenotypes. Here, we investigated the role of inflammation and genetic background for the establishment of ALD using two different mouse strains: C57BL/6 (B6) and A/J. B6 and A/J mice were treated with a high fat diet containing ethanol (HFDE) and compared to the controls for 10 weeks. Hepatomegaly and steatohepatitis were similar in B6 and A/J mice, but only A/J mice were resistant to weight gain. On the other hand, HFDE-fed B6 accumulated more triglycerides (TG) and cholesterol and presented more intense cellular infiltrate in the liver when compared to HFDM-fed mice. Liver inflammatory environment was distinct in these two mouse strains. While HFDE-fed B6 produced more liver IL-12, A/J mice increased the TNF-α production. We concluded that mouse genetic background could dictate the intensity of the HFDE-induced liver injury.
authors.affiliation: Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, 05508-900 São Paulo, SP, Brazil ; Instituto Carlos Chagas, Fundação Oswaldo Cruz, 81350-010 Curitiba, PR, Brazil.
authors.forename: Lorena
authors.initials: L
authors.lastname: Bavia
authors.affiliation: Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, 05508-900 São Paulo, SP, Brazil.
authors.forename: Íris Arantes
authors.initials: ÍA
authors.lastname: de Castro
authors.affiliation: Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, 05508-900 São Paulo, SP, Brazil.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Isaac
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D007249:Inflammation / Q000097:blood* / Q000276:immunology*; D018664:Interleukin-12 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007962:Leukocytes; D050356:Lipid Metabolism; D008099:Liver / Q000276:immunology / Q000473:pathology; D008108:Liver Diseases, Alcoholic / Q000097:blood / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D002784:Cholesterol