title: Aluminium stress disrupts metabolic performance of Plantago almogravensis plantlets transiently.
abstract: Little is known about how tolerant plants cope with internalized aluminium (Al). Tolerant plants are known to deploy efficient detoxification mechanisms, however it is not known to what extent the primary and secondary metabolism is affected by Al. The aim of this work was to study the metabolic repercussions of Al stress in the tolerant plant Plantago almogravensis. P. almogravensis is well adapted to acid soils where high concentrations of free Al are found and has been classified as a hyperaccumulator. In vitro reared plantlets were used for this purpose in order to control Al exposure rigorously. The metabolome of P. almogravensis plantlets as well as its metabolic response to the supply of sucrose was characterized. The supply of sucrose leads to an accumulation of amino acids and secondary metabolites and consumption of carbohydrates that result from increased metabolic activity. In Al-treated plantlets the synthesis of amino acids and secondary metabolites is transiently impaired, suggesting that P. almogravensis is able to recover from the Al treatment within the duration of the trials. In the presence of Al the consumption of carbohydrate resources is accelerated. The content of some metabolic stress markers also demonstrates that P. almogravensis is highly adapted to Al stress.
authors.affiliation: MeditBio, Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
authors.forename: Tomás
authors.initials: T
authors.lastname: Grevenstuk
authors.affiliation: INRA, UMR1332 Biologie du Fruit & Pathologie, Centre INRA de Bordeaux, 33140, Villenave d' Ornon, France.
authors.forename: Annick
authors.initials: A
authors.lastname: Moing
authors.affiliation: UMR1332 Biologie du Fruit & Pathologie, Centre INRA de Bordeaux, Univ Bordeaux, 33140, Villenave d'Ornon, France.|Metabolome Facility of Bordeaux Functional Genomics Centre, MetaboHUB, Centre INRA de Bordeauxb, IBVM, 33140, Villenave d'Ornon, France.
authors.forename: Mickaël
authors.initials: M
authors.lastname: Maucourt
authors.affiliation: INRA, UMR1332 Biologie du Fruit & Pathologie, Centre INRA de Bordeaux, 33140, Villenave d' Ornon, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Deborde
authors.affiliation: MeditBio, Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal. aromano@ualg.pt.
authors.forename: Anabela
authors.initials: A
authors.lastname: Romano
mesh_terms: D000222:Adaptation, Physiological; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000494:pharmacology*; D000596:Amino Acids / Q000378:metabolism; D001692:Biological Transport; D002712:Chlorides / Q000494:pharmacology*; D055442:Metabolome / Q000187:drug effects* / Q000502:physiology; D018517:Plant Roots / Q000187:drug effects* / Q000378:metabolism; D010943:Plantago / Q000187:drug effects* / Q000378:metabolism; D025341:Principal Component Analysis; D012987:Soil / Q000737:chemistry; D013312:Stress, Physiological; D013395:Sucrose / Q000494:pharmacology; D046509:Tissue Culture Techniques
chemical_list: D017607:Aluminum Compounds; D000596:Amino Acids; D002712:Chlorides; D012987:Soil; D000077410:Aluminum Chloride; D013395:Sucrose
keywords: Accumulation; Aluminium tolerance; In vitro culture; Metabolomics; Nuclear magnetic resonance