title: A new classification of interphase nuclei based on spatial organizations of chromosome 8 and 21 for t(8;21) (q22;q22) acute myeloid leukemia by three-dimensional fluorescence in situ hybridization.
abstract: Interphase heterogenous chromosomes spatially close to each other are predominantly located near the center of nuclei and are prone to incur translocations. We screened a t(8;21) (q22;q22) acute myeloid leukemia-M2 patient during three phases (post-chemotherapy, remittent stage, and relapse) and a donor of normal karyotype as control by two-(2D) and three-dimensional (3D)-fluorescence in situ hybridization (FISH). Our classification of nuclei (normal, transitional, and malignant nuclei) by 3D-FISH analyses may provide a more precise prognosis than 2D-FISH results, especially for remittent stage sample in our study, in which 2D-FISH findings showed normal results, whereas 3D-FISH results showed extreme abnormalities (normal nuclei 27%, transitional nuclei 36%, malignant nuclei 37%). The relative radial positions (d/R) of chromosomes 8 were similar to d/R of chromosomes 21 for the relapse sample. We classified heterogenous chromosome pairs into close pairs and normal pairs based on their relative distances (d'/(2R)). The centers of close pairs were more internal than normal pairs in nuclei in all samples, and the d/R values of a given-type pairwise heterogenous chromosomes were similar among four samples. Our data demonstrate that the classification of nuclei based on spatial organization of chromosomes by 3D-FISH is reasonable and essential for evaluating acute myeloid leukemia prognosis.
authors.affiliation: State Key Laboratory of Low-Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing, China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Low-Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing, China.
authors.forename: Fengying
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Automation, Tsinghua University, Beijing, China.
authors.forename: Jinlin
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Low-Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Low-Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing, China.
authors.forename: Dieyan
authors.initials: D
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Low-Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing, China; Collaborative Innovation Center of Quantum Matter, Beijing, China. Electronic address: mawy@mail.tsinghua.edu.cn.
authors.forename: Wanyun
authors.initials: W
authors.lastname: Ma
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing, China. Electronic address: xiaoyank@yahoo.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ke
mesh_terms: D001854:Bone Marrow Cells / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure*; D002891:Chromosomes, Human, Pair 21 / Q000648:ultrastructure*; D002898:Chromosomes, Human, Pair 8 / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D011379:Prognosis; D014178:Translocation, Genetic
keywords: 3D-FISH; Acute myeloid leukemia; Chromosome pair; Prognosis; Translocation