title: Duganella ginsengisoli sp. nov., isolated from ginseng soil.
abstract: A Gram-stain-negative, rod-shaped bacterium, designated DCY83T, was isolated from soil of a ginseng field in Gwangju Province, Republic of Korea. Cells were motile by means of flagella. Growth occurred at 4-40 °C (optimum 30 °C), at pH 6-8 (optimum pH 7.0) and with ≤ 0.4 % NaCl. Strain DCY83T was able to produce siderophore and was positive for phosphate solubilization. Indole-3-acetic acid production was 12.9 μg ml- 1 after 3 days in culture. 16S rRNA gene sequence analysis showed that strain DCY83T belonged to the genus Duganella and was related most closely to Duganella sacchari Sac-22T (97.4 % similarity), Duganella zoogloeoides IAM 12670T (97.1 %) and Duganella radicis Sac-41T (97.1 %). The major fatty acids were C16 : 0 and summed feature 3 (containing C16 : 1ω7c and/or C16 : 1ω6c). The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The only quinone was ubiquinone 8. The genomic DNA G+C content was 55.3 mol%. DNA-DNA relatedness between strain DCY83T and D. sacchari KCTC 22381T, D. zoogloeoides JCM 20729T and D. radicis KCTC 22382T was 27.7, 22.4 and 35.5 %, respectively. On the basis of the phenotypic and genotypic analysis, DCY83T is classified as representing a novel species in the genus Duganella, for which the name Duganella ginsengisoli sp. nov. is proposed. The type strain is DCY83T ( = KCTC 42409T = JCM 30745T).
authors.affiliation: Graduate School of Biotechnology, Ginseng Genetic Resource Bank, College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Jinglou
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung-Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Yeon-Ju
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung-Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Van-An
authors.initials: VA
authors.lastname: Hoang
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung-Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Ngoc
authors.initials: N
authors.lastname: Lan Nguyen
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung-Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung-Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Jong-Pyo
authors.initials: JP
authors.lastname: Kang
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung-Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Dandan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Graduate School of Biotechnology, Ginseng Genetic Resource Bank, College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea.|Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung-Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Deok-Chun
authors.initials: DC
authors.lastname: Yang
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D042802:Oxalobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005894:Panax / Q000382:microbiology*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C483858:phosphatidylethanolamine; C030778:ubiquinone 8