title: Tailored Fringed Platforms Produced by Laser Interference for Aligned Neural Cell Growth.
abstract: Ordering neural cells is of interest for the development of neural interfaces. The aim of this work is to demonstrate an easy-to-use, versatile, and cost/time effective laser-based approach for producing platforms that promote oriented neural growth. We use laser interferometry to generate fringed channels with topography on partially reduced graphene oxide layers as a proof-of-concept substrate. We study cell adhesion, morphology, viability, and differentiation in cultures of embryonic neural progenitor cells on platforms with a 9.4 μm period. Results evidence that fringed platforms significantly promote neurite alignment (≈50% at 6 d), while preserving viability and neural differentiation.
authors.affiliation: Laser Processing Group, Instituto de Óptica, Consejo Superior de Investigaciones Científicas (CSIC), Calle Serrano 121, 28006-Madrid, Spain.
authors.forename: Ramón J
authors.initials: RJ
authors.lastname: Peláez
authors.affiliation: Laboratory of Neural Repair and Biomaterials, Hospital Nacional de Parapléjicos, Servicio de Salud de Castilla La Mancha, Finca La Peraleda s/n, 45071-Toledo, Spain.
authors.forename: Ankor
authors.initials: A
authors.lastname: González-Mayorga
authors.affiliation: Group of Bioinspired Materials, Instituto de Ciencia de Materiales de Madrid, Consejo Superior de Investigaciones Científicas, Calle Sor Juana Inés de la Cruz 3, 28049-Madrid, Spain.
authors.forename: María C
authors.initials: MC
authors.lastname: Gutiérrez
authors.affiliation: Laboratory of Neural Repair and Biomaterials, Hospital Nacional de Parapléjicos, Servicio de Salud de Castilla La Mancha, Finca La Peraleda s/n, 45071-Toledo, Spain.
authors.forename: Concepción
authors.initials: C
authors.lastname: García-Rama
authors.affiliation: Laser Processing Group, Instituto de Óptica, Consejo Superior de Investigaciones Científicas (CSIC), Calle Serrano 121, 28006-Madrid, Spain.
authors.forename: Carmen N
authors.initials: CN
authors.lastname: Afonso
authors.affiliation: Laboratory of Neural Repair and Biomaterials, Hospital Nacional de Parapléjicos, Servicio de Salud de Castilla La Mancha, Finca La Peraleda s/n, 45071-Toledo, Spain. mslopezterradas@sescam.jccm.es.
authors.forename: María C
authors.initials: MC
authors.lastname: Serrano
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D004622:Embryo, Mammalian / Q000378:metabolism*; D007834:Lasers; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism*; D051381:Rats
keywords: cell alignment; graphene oxide; laser interference; neural cells; pattern