title: Fingerprint analysis and multi-component determination of Zibu Piyin recipe by HPLC with DAD and Q-TOF/MS method.
abstract: Zibu Piyin recipe (ZBPYR), a traditional Chinese medicine formula, is used for curing dementia caused by diabetes. For quality control of ZBPYR, ﬁngerprint analysis and qualitative analysis using high-performance liquid chromatography (HPLC) with a diode-array detector, and confirmation using HPLC coupled with electrospray ionisation quadrupole time-of-ﬂight tandem mass spectrometry (HPLC-Q-TOF-MS) were undertaken. HPLC fingerprint consisting of 34 common peaks was developed among 10 batches of ZBPYR, in which 7 common peaks were identified in comparison with the authentic standards and detected simultaneously. Furthermore, these seven compounds were verified by HPLC-Q-TOF-MS methods. The method can be applied to the quality control of ZBPYR.
authors.affiliation: a Department of Traditional Chinese Medicine, the Second Affiliated Hospital , Dalian Medical University , Dalian , China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xiang
authors.affiliation: a Department of Traditional Chinese Medicine, the Second Affiliated Hospital , Dalian Medical University , Dalian , China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Xu
authors.affiliation: a Department of Traditional Chinese Medicine, the Second Affiliated Hospital , Dalian Medical University , Dalian , China.|b School of Basic Medical Sciences , Nanjing University of Chinese Medicine , Nanjing , China.
authors.forename: Libin
authors.initials: L
authors.lastname: Zhan
authors.affiliation: c Institute of Integrative Medicine , Dalian Medical University , Dalian , China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D011786:Quality Control; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; C531140:zibu piyin
keywords: HPLC-DAD; HPLC-Q-TOF-MS; ZBPYR; fingerprint analysis; quality determination