title: Exploiting the ubiquitin and phosphoinositide pathways by the Legionella pneumophila effector, SidC.
abstract: Intracellular bacterial pathogens use secreted effector proteins to alter host cellular processes, with the goal of subverting host defenses and allowing the infection to progress. One such pathogen, Legionella pneumophila, secretes ~300 proteins into its host to alter a number of pathways including intracellular trafficking, phosphoinositide metabolism, and cell signaling. The Legionella effector SidC was previously found to bind to PI(4)P and was responsible for the enrichment of ER proteins and ubiquitinated species on the Legionella-containing vacuoles. Through our recent work, we have discovered that SidC contains a unique N-terminal E3 ubiquitin ligase domain and a C-terminal novel PI(4)P-binding domain. Our results demonstrate that SidC serves to link two distinct cellular pathways, ubiquitin and phosphoinositide. However, how the ubiquitin ligase activity regulates host membrane trafficking events remains to be investigated.
authors.affiliation: Weill Institute for Cell and Molecular Biology and Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Wasilko
authors.affiliation: Weill Institute for Cell and Molecular Biology and Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, 14853, USA. ym253@cornell.edu.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Mao
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001692:Biological Transport; D016952:Legionella pneumophila / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D011485:Protein Binding; D015398:Signal Transduction; D025801:Ubiquitin / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D001426:Bacterial Proteins; D010716:Phosphatidylinositols; C534216:SidC protein, Legionella pneumophila; D025801:Ubiquitin
keywords: Intracellualr bacterial pathogen; PI(4)P; T4SS; Ubiquiin ligase