title: Ovariectomized rats' femur treated with fibrates and statins. Assessment of pore-size distribution by ¹H-NMR relaxometry.
abstract: The effects of two wonder drugs, simvastatins and fenofibrates on the proximal part of the femoris of a series of ovariectomized and non-ovariectomized Wistar albino rats was estimated qualitatively and semi-quantitatively by the modern method of 1D 1H-NMR T2-distribution. The 72 rats subjected to this study were divided in six groups and were sacrificed at two, four, six and eight weeks after ovariectomy and the proximal part of femoris was harvested. The CPMG (Carr-Purcell-Meiboom-Gill) echoes train curves were measured for the bones fully saturated with water during two months after two months of natural drying. These decays were analyzed by Laplace inversion and an average of normalized T2-distributions was considered for all rat's groups. The 1D averaged T2-distributions present four peaks, which were associated with protons in four major environments, from which the free water protons are used as spy molecules to explore the boundaries of cavities. In the approximation of spherical pores, the averaged T2-distributions were transformed in distributions of pores diameters. These were found in the range from 2 μm up to 2 mm. The relative amplitudes, widths and position of deconvoluted distributions of small, medium and large cavities are used for a qualitatively analysis of the effect of our lipid-lowering drugs. For a semi-quantitatively analysis, we chose the diameter d of proximal part of femoris' trabecular cavities. We show that the positive or negative effects of treatments with simvastatins and fenofibrates are strongly dependent on the duration of treatment. Moreover, the treatment of healthy bone is generally counter-indicated.
authors.affiliation: Department of Morphological Sciences, University of Medicine and Pharmacy of Tirgu Mures, Romania; dr_sipos@yahoo.com; Department of Physics and Chemistry, Technical University of Cluj-Napoca, Romania; rfechete@phys.utcluj.ro.
authors.forename: Remus Sebastian
authors.initials: RS
authors.lastname: Şipoş
authors.forename: Radu
authors.initials: R
authors.lastname: Fechete
authors.forename: Ramona Ioana
authors.initials: RI
authors.lastname: Chelcea
authors.forename: Dumitriţa
authors.initials: D
authors.lastname: Moldovan
authors.forename: Zsuzsánna
authors.initials: Z
authors.lastname: Pap
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Pávai
authors.forename: Dan Eugen
authors.initials: DE
authors.lastname: Demco
mesh_terms: D000818:Animals; D005260:Female; D005269:Femur / Q000187:drug effects* / Q000473:pathology; D011345:Fenofibrate / Q000494:pharmacology; D058607:Fibric Acids / Q000494:pharmacology*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000494:pharmacology; D010052:Ovariectomy; D066244:Proton Magnetic Resonance Spectroscopy; D051381:Rats; D017208:Rats, Wistar; D019821:Simvastatin / Q000494:pharmacology
chemical_list: D058607:Fibric Acids; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D000960:Hypolipidemic Agents; D019821:Simvastatin; D011345:Fenofibrate