title: A strategy for dissecting the architectures of native macromolecular assemblies.
abstract: It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.
authors.affiliation: Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.|Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA.|California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, California, USA.|Institut Pasteur, Paris, France.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Pellarin
authors.affiliation: Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, New York, USA.
authors.forename: Peter C
authors.identifier: http://orcid.org/0000-0002-8208-9154
authors.initials: PC
authors.lastname: Fridy
authors.affiliation: Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, New York, USA.
authors.forename: Javier
authors.initials: J
authors.lastname: Fernandez-Martinez
authors.affiliation: Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, New York, USA.
authors.forename: Mary K
authors.initials: MK
authors.lastname: Thompson
authors.affiliation: Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York, USA.
authors.forename: Yinyin
authors.initials: Y
authors.lastname: Li
authors.forename: Qing Jun
authors.initials: QJ
authors.lastname: Wang
authors.affiliation: Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.|Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA.|California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Andrej
authors.initials: A
authors.lastname: Sali
authors.affiliation: Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, New York, USA.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Rout
authors.affiliation: Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York, USA.
authors.forename: Brian T
authors.initials: BT
authors.lastname: Chait
mesh_terms: D000818:Animals; D003432:Cross-Linking Reagents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D055354:Exosomes / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics; D008099:Liver / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008822:Mice, Transgenic; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D003432:Cross-Linking Reagents; D046912:Multiprotein Complexes; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins