title: Effect of pulsed electromagnetic field therapy on the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells.
abstract: We investigated the effects of pulsed electromagnetic fields (PEMFs) of 20 Hz/2 mT on the osteogenic and adipogenic differentiation of bone marrow stem cells (BMSCs). Sprague Dawley rat BMSCs were isolated and cultured in vitro. The BMSCs of the third passage were obtained and stimulated by PEMFs of 20 Hz/2 mT. The alkaline phosphatase (ALP) activity was measured according to the ALP assay kit manufacturer instructions, the BMSC osteogenic and adipogenic indicators were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), and oil red O staining was used to observe the adipose-induced adipogenic differentiation of BMSCs. PEMFs of 20 Hz/2 mT significantly promoted the activity of ALP in the BMSCs (P < 0.01) and mRNA expression of osteogenic proteins (osteocalcin and osteopontin). The PEMFs inhibited the expression of adipogenic transcription factors such as adipokines and adipocyte-binding protein-2, and the adipogenic differentiation of BMSCs. PEMFs of 20 Hz/2 mT can promote osteogenic differentiation and inhibit adipogenic differentiation in BMSCs.
authors.affiliation: Second Department of Orthopedic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
authors.forename: T
authors.initials: T
authors.lastname: Lu
authors.affiliation: First Department of Orthopedic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Huang
authors.affiliation: Second Department of Orthopedic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Second Department of Orthopedic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
authors.forename: M X
authors.initials: MX
authors.lastname: Chai
authors.affiliation: Second Department of Orthopedic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D004574:Electromagnetic Fields; D005260:Female; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis / Q000235:genetics; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D013194:Staining and Labeling
chemical_list: D000469:Alkaline Phosphatase