title: Effects on MC3T3-E1 Cells and In silico Toxicological Study of Two 6-(Propan-2-yl)-4-methyl-morpholine-2,5-diones.
abstract: Recently, we found that two cyclodidepsipeptides, 3,6-di-(propan-2-yl)-4-methyl-morpholine-2,5-dione (1) and 3-(2-methylpropyl)-6-(propan-2-yl)-4-methyl- morpholine-2,5-dione (2), are excellent inhibitors of xanthine oxidase. In order to obtain more information about the toxicological potential of compounds 1 and 2 on bone cells, the current study was designed to evaluate the effect of these compounds on viability and proliferation of MC3T3-E1 cells. Compound 1 showed neither cytotoxic nor stimulatory effect on cell viability, while compound 2 showed a slight stimulatory effect on cell viability. Both studied compounds showed slight stimulatory effects on proliferation of MC3T3-E1 cells, in a dose dependent manner. Additionally, an in silico toxicological study of compounds 1 and 2 was performed, and the results indicate that they have a good probability of safe biological intake.
authors.forename: Marija
authors.initials: M
authors.lastname: Vukelić-Nikolić
authors.forename: Ana
authors.initials: A
authors.lastname: Kolarevć
authors.forename: Katarina
authors.initials: K
authors.lastname: Tomović
authors.forename: Denitsa
authors.initials: D
authors.lastname: Yancheva
authors.forename: Emiliya
authors.initials: E
authors.lastname: Cherneva
authors.forename: Stevo
authors.initials: S
authors.lastname: Najman
authors.forename: Andrija
authors.initials: A
authors.lastname: Smelcerović
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000633:toxicity*; D051379:Mice; D009025:Morpholines / Q000633:toxicity*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D014969:Xanthine Oxidase / Q000032:analysis
chemical_list: D004791:Enzyme Inhibitors; D009025:Morpholines; C520636:morpholine-2,5-dione; D014969:Xanthine Oxidase