title: Elevated IL-35 in bone marrow of the patients with acute myeloid leukemia.
abstract: Acute myeloid leukemia (AML) is the most common hematological malignancy in adults, but the etiology of it remains poorly understood. IL-35 is a recently described cytokine composed of an IL-12 subunit p35 and an IL-27 subunit Epstein-Barr virus induced gene 3 (EBI3), and has an immunosuppressive effect on inflammation through induction of regulatory T cells (Tregs) and suppression of Th1 and Th17. Recently, we have illustrated that concentrations of IL-35 in peripheral blood are up-regulated in newly diagnosed (ND) AML patients. However, whether IL-35 in bone marrow is increased in AML patients is not clear. In this study, we examined IL-35 in bone marrow by various methods including RT-PCR, ELISA, FCM and IHC, and found that IL-35 levels are also increased significantly in bone marrow of adult AML patients. Furthermore, we investigated that concentrations of bone marrow IL-35 in ND group were higher than that in complete remission (CR) group and control group, but there was no significant difference compared to that in relapse group. In conclusion, IL-35 was elevated in bone marrow of adult AML patients and this increase was correlated with the clinical stages of malignancy, suggesting that IL-35 is involved in pathogenesis of AML.
authors.affiliation: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, China; Hematology Research Center, Anhui Medical University, Hefei, Anhui 230601, China.
authors.forename: Qianshan
authors.initials: Q
authors.lastname: Tao
authors.affiliation: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, China; Hematology Research Center, Anhui Medical University, Hefei, Anhui 230601, China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, China.
authors.forename: Zhitao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, China; Hematology Research Center, Anhui Medical University, Hefei, Anhui 230601, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Wu
authors.affiliation: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, China; Hematology Research Center, Anhui Medical University, Hefei, Anhui 230601, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, China; Hematology Research Center, Anhui Medical University, Hefei, Anhui 230601, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Tao
authors.affiliation: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, China; Hematology Research Center, Anhui Medical University, Hefei, Anhui 230601, China.
authors.forename: Shudao
authors.initials: S
authors.lastname: Xiong
authors.affiliation: Centre of Transplantation and Renal Research Westmead Millennium Institute, Sydney University, Sydney, Australia.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, China; Hematology Research Center, Anhui Medical University, Hefei, Anhui 230601, China. Electronic address: zzzm889@163.com.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Zhai
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology; D002869:Chromosome Aberrations; D005260:Female; D015870:Gene Expression; D006801:Humans; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger; D055815:Young Adult
chemical_list: D007378:Interleukins; D012333:RNA, Messenger; C532417:interleukin-35, human
keywords: Acute myeloid leukemia; Bone marrow; IL-35; Immune escape