title: Cercosporoid diseases of Citrus.
abstract: Citrus leaves and fruits exhibiting disease symptoms ranging from greasy spot, yellow spot, small or large brown spot, black dot, and brown dot were sampled from Fujian, Guangdong, Guizhou, Hunan, Jiangxi, Yunnan, Zhejiang provinces and the Guangxi Zhuang Autonomous Region in China. In total 82 isolates representing various cercosporoid genera were isolated from these disease symptoms, which were supplemented with eight Citrus cercosporoid isolates collected from other countries. Based on a morphological and phylogenetic study using sequence data from the nuclear ribosomal DNA's ITS1-5.8S-ITS2 regions (ITS), and partial actin (act), β-tubulin (tub2), 28S nuclear ribosomal RNA (28S rDNA) and translation elongation factor 1-α (tef1) genes, these strains were placed in the following genera: Cercospora, Pallidocercospora, Passalora, Pseudocercospora, Verrucisporota and Zasmidium. All isolates tended to be sterile, except the Zasmidium isolates associated with citrus greasy spot-like symptoms, which subsequently were compared with phylogenetically similar isolates occurring on Citrus and other hosts elsewhere. From these results four Zasmidium species were recognized on Citrus, namely Z. indonesianum on Citrus in Indonesia, Z. fructicola and Z. fructigenum on Citrus in China and Z. citri-griseum, which appears to have a wide host range including Acacia, Citrus, Eucalyptus and Musa, as well as a global distribution.
authors.affiliation: Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Huang
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, the Netherlands.
authors.forename: J Z
authors.initials: JZ
authors.lastname: Groenewald
authors.affiliation: Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, the Netherlands, Department of Plant Pathology and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South AfricaMicrobiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands p.crous@cbs.knaw.nl hyli@zju.edu.cn.
authors.forename: P W
authors.initials: PW
authors.lastname: Crous
authors.affiliation: Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China p.crous@cbs.knaw.nl hyli@zju.edu.cn.
authors.forename: Hongye
authors.initials: H
authors.lastname: Li
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D002681:China; D002957:Citrus / Q000382:microbiology*; D005656:Fungal Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D014404:Tubulin / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D020648:Peptide Elongation Factor 1; D014404:Tubulin
keywords: Mycosphaerella; Pseudocercospora; Zasmidium; citrus greasy spot; multigene phylogenetic analyses