title: Molecularly imprinted solid-phase extraction coupled to liquid chromatography for determination of Sudan dyes in preserved beancurds.
abstract: New molecularly imprinted microspheres synthesized by suspension polymerisation using phenylamine and naphthol as mimic template were successfully applied as selective sorbents for the solid-phase extraction used for the simultaneous determination of four Sudan dyes from preserved beancurd products. The obtained imprinted microspheres showed good recognition and selectivity to the four Sudan dyes in aqueous solution and the affinity could be easily controlled by adjusting the property of the solution. Under the selected experimental condition, the recoveries of the Sudan dyes in preserved beancurds at three spiked levels were ranged between 90.2-104.5% with the relative standard deviation of less than 6.8%. The limit of detection (LOD) and limit of quantification (LOQ) based on a signal-to-noise of 3 and 10 were in the range of 0.005-0.009μgg(-1) and 0.015-0.030μgg(-1), respectively. Comparing with alumina and C18-based extraction, the selectivity and repeatability of molecularly imprinted solid-phase extraction (MISPE) were obviously improved. This method could be potentially applied for the determination of Sudan dyes in complicated food samples.
authors.affiliation: Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University, Baoding 071002, China. Electronic address: yanhy@hbu.edu.cn.
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Yan
authors.affiliation: Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University, Baoding 071002, China.
authors.forename: Jindong
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University, Baoding 071002, China.
authors.forename: Yuning
authors.initials: Y
authors.lastname: Pei
authors.affiliation: Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University, Baoding 071002, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Long
authors.affiliation: Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University, Baoding 071002, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Ding
authors.affiliation: Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University, Baoding 071002, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xie
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid; D004396:Coloring Agents / Q000032:analysis / Q000737:chemistry*; D008863:Microspheres; D054802:Molecular Imprinting / Q000379:methods*; D015394:Molecular Structure; D009284:Naphthols / Q000032:analysis; D052616:Solid Phase Extraction / Q000379:methods*; D045730:Soy Foods / Q000032:analysis*
chemical_list: D004396:Coloring Agents; D009284:Naphthols
keywords: Molecularly imprinted microsphere; Preserved beancurds; Solid-phase extraction; Sudan dyes