title: Symbiosis dependent accumulation of primary metabolites in arbuscule-containing cells.
abstract: BACKGROUND
  The arbuscular mycorrhizal symbiosis is characterized by the presence of different symbiotic structures and stages within a root system. Therefore tools allowing the analysis of molecular changes at a cellular level are required to reveal insight into arbuscular mycorrhizal (AM) symbiosis development and functioning.
  
  
  RESULTS
  Here we describe the analysis of metabolite pools in arbuscule-containing cells, which are the site of nutrient transfer between AM fungus and host plant. Laser capture microdissection (LCM) combined with gas chromatography mass spectrometry (GC-EI/TOF-MS) enabled the analysis of primary metabolite levels,which might be of plant or fungal origin, within these cells.
  
  
  CONCLUSIONS
  High levels of the amino acids, aspartate, asparagine, glutamate, and glutamine, were observed in arbuscule-containing cells. Elevated amounts of sucrose and the steady-state of hexose levels indicated a direct assimilation of monosaccharides by the fungal partner.
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476, Potsdam, Germany. Gaude@mpimp-golm.mpg.de.
authors.forename: Nicole
authors.initials: N
authors.lastname: Gaude
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476, Potsdam, Germany. Silvia_Bortfeld@t-online.de.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bortfeld
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476, Potsdam, Germany. Erban@mpimp-golm.mpg.de.
authors.forename: Alexander
authors.initials: A
authors.lastname: Erban
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476, Potsdam, Germany. Kopka@mpimp-golm.mpg.de.
authors.forename: Joachim
authors.initials: J
authors.lastname: Kopka
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476, Potsdam, Germany. Krajinski@mpimp-golm.mpg.de.
authors.forename: Franziska
authors.initials: F
authors.lastname: Krajinski
mesh_terms: D002244:Carbon / Q000378:metabolism; D046913:Medicago truncatula / Q000166:cytology* / Q000382:microbiology*; D055442:Metabolome; D055432:Metabolomics; D038821:Mycorrhizae / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D013312:Stress, Physiological; D013559:Symbiosis
chemical_list: D010710:Phosphates; D002244:Carbon; D009584:Nitrogen