title: Nuclease stability of boron-modified nucleic acids: application to label-free mismatch detection.
abstract: 5'-End boronic acid-modified oligonucleotides were evaluated against various nucleases at single and double stranded levels. The results show that these modifications induce a high resistance to degradation by calf-spleen and snake venom phosphodiesterases. More importantly, this eventually led to the development of a new label-free enzyme-assisted fluorescence-based method for single mismatch detection.
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM) UMR 5247 CNRS-Université de Montpellier-ENSCM, Place Bataillon, 34095 Montpellier, France. msmietana@um2.fr.
authors.forename: Maëva
authors.initials: M
authors.lastname: Reverte
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM) UMR 5247 CNRS-Université de Montpellier-ENSCM, Place Bataillon, 34095 Montpellier, France. msmietana@um2.fr.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Vasseur
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM) UMR 5247 CNRS-Université de Montpellier-ENSCM, Place Bataillon, 34095 Montpellier, France. msmietana@um2.fr.
authors.forename: Michael
authors.initials: M
authors.lastname: Smietana
mesh_terms: D000818:Animals; D020137:Base Pair Mismatch; D001483:Base Sequence; D001897:Boronic Acids / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D004720:Endonucleases / Q000378:metabolism*; D009696:Nucleic Acids / Q000737:chemistry / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D012911:Snakes; D013050:Spectrometry, Fluorescence; D013154:Spleen / Q000201:enzymology
chemical_list: D001897:Boronic Acids; D009696:Nucleic Acids; D009841:Oligonucleotides; D004720:Endonucleases; D010727:Phosphoric Diester Hydrolases