title: Pseudoxanthomonas humi sp. nov., a bacterium isolated from rhizospheric soil of Fraxinus chinensis in Gyeonggi Province, South Korea.
abstract: A novel bacterial strain THG-MM13(T) was isolated from rhizospheric soil sample and was characterized by using a polyphasic approach. Cells were Gram-reaction-negative, non-motile and rod-shaped. The strain was aerobic, catalase and oxidase positive, and optimum growth temperature and pH were 28 °C and 7.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain THG-MM13(T) (KM598260) belongs to the genus Pseudoxanthomonas and is most closely related to Pseudoxanthomonas wuyuanensis KCTC 23877(T) (97.4 %) (JN247803), followed by Pseudoxanthomonas koreensis KCTC 12208(T) (96.7 %) (AY550263) and Pseudoxanthomonas yeongjuensis KACC 11580(T) (96.7 %) (DQ438977). The DNA G + C content was 63.7 mol%, and the predominant respiratory quinone was ubiquinone-8. The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were iso-C15:0 (31.3 %) and iso-C16:0 (19.3 %). The DNA-DNA relatedness value between strain THG-MM13(T) and P. wuyuanensis KCTC 23877(T) was below 50 %. The DNA-DNA hybridization result and results of the genotypic analysis in combination with chemotaxonomic and physiological data demonstrated that strain THG-MM13(T) represented a novel species within the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas humi is proposed. The type strain is THG-MM13(T) (=KACC 18280(T) = CCTCC AB 2015122(T)).
authors.affiliation: Department of Oriental Medicinal Material and Processing College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Gyeonggi-do, Yongin-si, 446-701, Republic of Korea.
authors.forename: Shahina
authors.initials: S
authors.lastname: Akter
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, Gyeonggi-do, Republic of Korea.
authors.forename: Hae
authors.initials: H
authors.lastname: Jo
authors.affiliation: Department of Oriental Medicinal Material and Processing College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Gyeonggi-do, Yongin-si, 446-701, Republic of Korea.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Oriental Medicinal Material and Processing College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Gyeonggi-do, Yongin-si, 446-701, Republic of Korea.
authors.forename: KyungHwa
authors.initials: K
authors.lastname: Won
authors.affiliation: Department of Acupuncture Meridian Science Research Center, College of Korean Medicine, Kyung Hee University Global Campus, Seoul, Republic of Korea.
authors.forename: Chang Shik
authors.initials: CS
authors.lastname: Yin
authors.affiliation: Department of Marine Biotechnology, Anyang University, Incheon, 417-833, Republic of Korea.
authors.forename: MooChang
authors.initials: M
authors.lastname: Kook
authors.affiliation: College of Bio and Food Technology, Dalian Polytechnic University, Qinggong-yuan No. 1, Ganjingzi-qu, Dalia, 116034, People's Republic of China.
authors.forename: Hongshan
authors.initials: H
authors.lastname: Yu
authors.affiliation: Division of Fermented Food Science, National Academy of Agriculture Science, RDA, Wanju, 565-851, Republic of Korea.
authors.forename: Han-Seok
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Department of Food Science and Technology, Chonbuk National University, Jeonju, 561-756, Republic of Korea.
authors.forename: Myung-Kon
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Department of Oriental Medicinal Material and Processing College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Gyeonggi-do, Yongin-si, 446-701, Republic of Korea. drhoo@khu.ac.kr.|Graduate School of Biotechnology, Kyung Hee University, Gyeonggi-do, Republic of Korea. drhoo@khu.ac.kr.
authors.forename: Tae-Hoo
authors.identifier: http://orcid.org/0000-0001-9369-6542
authors.initials: TH
authors.lastname: Yi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D031661:Fraxinus / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D044166:Xanthomonadaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA; Gram-reaction-negative; Pseudoxanthomonas humi; Ubiquinone-8