title: Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia.
abstract: Dynamic changes in tissue water diffusion and glucose metabolism occur during and after hypoxia in cerebral hypoxia-ischemia reflecting a bioenergetics disturbance in affected cells. Diffusion weighted magnetic resonance imaging (MRI) identifies regions that are damaged, potentially irreversibly, by hypoxia-ischemia. Alterations in glucose utilization in the affected tissue may be detectable by positron emission tomography (PET) imaging of 2-deoxy-2-(18F)fluoro-ᴅ-glucose ([18F]FDG) uptake. Due to the rapid and variable nature of injury in this animal model, acquisition of both modes of data must be performed simultaneously in order to meaningfully correlate PET and MRI data. In addition, inter-animal variability in the hypoxic-ischemic injury due to vascular differences limits the ability to analyze multi-modal data and observe changes to a group-wise approach if data is not acquired simultaneously in individual subjects. The method presented here allows one to acquire both diffusion-weighted MRI and [18F]FDG uptake data in the same animal before, during, and after the hypoxic challenge in order to interrogate immediate physiological changes.
authors.affiliation: Department of Biomedical Engineering, University of California, Davis; youyang@ucdavis.edu.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ouyang
authors.affiliation: Department of Biomedical Engineering, University of California, Davis.
authors.forename: Martin S
authors.initials: MS
authors.lastname: Judenhofer
authors.affiliation: Department of Biomedical Engineering, University of California, Davis; Nuclear Magnetic Resonance Facility, University of California, Davis.
authors.forename: Jeffrey H
authors.initials: JH
authors.lastname: Walton
authors.affiliation: Biomedical Imaging, Genentech, Inc.
authors.forename: Jan
authors.initials: J
authors.lastname: Marik
authors.affiliation: Biomedical Imaging, Genentech, Inc.
authors.forename: Simon P
authors.initials: SP
authors.lastname: Williams
authors.affiliation: Department of Biomedical Engineering, University of California, Davis; Department of Radiology, University of California, Davis.
authors.forename: Simon R
authors.initials: SR
authors.lastname: Cherry
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D019788:Fluorodeoxyglucose F18 / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D020925:Hypoxia-Ischemia, Brain / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D064847:Multimodal Imaging / Q000379:methods; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000032:analysis
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D005947:Glucose