title: Streptobacillus notomytis sp. nov., isolated from a  spinifex hopping mouse (Notomys alexis Thomas,  1922), and emended description of Streptobacillus  Levaditi et al. 1925, Eisenberg et al. 2015 emend.
abstract: A pleomorphic, Gram-negative, rod-shaped, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile bacterium was isolated in 1979 from the heart of a spinifex hopping mouse (Notomys alexis Thomas, 1922) with septicaemia and stored as Streptobacillus moniliformis in the strain collection of the Animal Health Laboratory, South Perth, Western Australia (AHL 370-1), as well as under CCUG 12425. On the basis of 16SrRNA gene sequence analyses, the strain was assigned to the genus Streptobacillus, with 99.4 % sequence similarity to the type strain of Streptobacillus moniliformis, 95.6 %sequence similarity to the type strain of Streptobacillus hongkongensis and 99.0 %sequence similarity to the type strain of Streptobacillus felis. The clear differentiation of strain AHL 370-1T from Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis was also supported by rpoB, groEL and recA nucleotide and amino acid sequence analysis. Average nucleotide identity was 87.16 % between strain AHL 370-1T and Streptobacillus moniliformis DSM 12112T. Physiological data confirmed the allocation of strain AHL 370-1T to the family Leptotrichiaceae, considering the very similar profiles of enzyme activities and fatty acids compared to closely related species. Within the genus Streptobacillus,isolate AHL 370-1T could also be separated unambiguously from the type strains of Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis by MALDI-TOF mass spectrometry. Two further strains (KWG2 and KWG24) isolated from asymptomatic black rats in Japan were highly similar to AHL 370-1T. On the basis of these data, we propose the novel species Streptobacillus notomytis sp. nov., with the type strain AHL370-1T (=CCUG 12425T=DSM 100026T=CCM 8593T=EF 12425T).
authors.affiliation: Landesbetrieb Hessisches Landeslabor, D-35392 Giessen, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Eisenberg
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Stefanie P
authors.initials: SP
authors.lastname: Glaeser
authors.affiliation: Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Christa
authors.initials: C
authors.lastname: Ewers
authors.affiliation: Robert Koch-Institut, D-13353 Berlin, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Semmler
authors.affiliation: Deutsches Krebsforschungszentrum, D-69120 Heidelberg, Germany.
authors.forename: Werner
authors.initials: W
authors.lastname: Nicklas
authors.affiliation: Chemisches und Veterinäruntersuchungsamt Stuttgart, D-70736 Fellbach, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Rau
authors.affiliation: Chemisches und Veterinäruntersuchungsamt Stuttgart, D-70736 Fellbach, Germany.
authors.forename: Norman
authors.initials: N
authors.lastname: Mauder
authors.affiliation: Institut für Mehrphasenprozesse, D-30167 Hannover, Germany.
authors.forename: Nicola
authors.initials: N
authors.lastname: Hofmann
authors.affiliation: Department of Veterinary Science, National Institute of Infectious Diseases, 162-8640 Tokyo, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Imaoka
authors.affiliation: Department of Veterinary Science, National Institute of Infectious Diseases, 162-8640 Tokyo, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kimura
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D006321:Heart / Q000382:microbiology; D007564:Japan; D008969:Molecular Sequence Data; D051189:Murinae / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D051381:Rats; D017422:Sequence Analysis, DNA; D013288:Streptobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014904:Western Australia
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S