title: Metabarcoding approach for nonindigenous species surveillance in marine coastal waters.
abstract: In this study, high-throughput sequencing (HTS) metabarcoding was applied for the surveillance of plankton communities within the southeastern (SE) Baltic Sea coastal zone. These results were compared with those from routine monitoring survey and morphological analyses. Four of five nonindigenous species found in the samples were identified exclusively by metabarcoding. All of them are considered as invasive in the Baltic Sea with reported impact on the ecosystem and biodiversity. This study indicates that, despite some current limitations, HTS metabarcoding can provide information on the presence of exotic species and advantageously complement conventional approaches, only requiring the same monitoring effort as before. Even in the currently immature status of HTS, this combination of HTS metabarcoding and observational records is recommended in the early detection of marine pests and delivery of the environmental status metrics of nonindigenous species.
authors.affiliation: Marine Science and Technology Centre, Klaipeda University, H. Manto 84, LT 92294 Klaipeda, Lithuania; Coastal and Freshwater Group, Cawthron Institute, 98 Halifax Street East, 7010 Nelson, New Zealand. Electronic address: anastasija@corpi.ku.lt.
authors.forename: Anastasija
authors.initials: A
authors.lastname: Zaiko
authors.affiliation: Marine Science and Technology Centre, Klaipeda University, H. Manto 84, LT 92294 Klaipeda, Lithuania.
authors.forename: Aurelija
authors.initials: A
authors.lastname: Samuiloviene
authors.affiliation: Marine Science and Technology Centre, Klaipeda University, H. Manto 84, LT 92294 Klaipeda, Lithuania; Department of Functional Biology, University of Oviedo, C/ Julian Claveria s/n., 33006 Oviedo, Spain.
authors.forename: Alba
authors.initials: A
authors.lastname: Ardura
authors.affiliation: Department of Functional Biology, University of Oviedo, C/ Julian Claveria s/n., 33006 Oviedo, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Garcia-Vazquez
mesh_terms: D000818:Animals; D044822:Biodiversity; D019295:Computational Biology; D003247:Conservation of Natural Resources; D004247:DNA / Q000737:chemistry; D017753:Ecosystem; D059014:High-Throughput Nucleotide Sequencing; D058865:Introduced Species; D009792:Oceans and Seas; D012426:Russia; D015048:Zooplankton / Q000145:classification / Q000235:genetics*
chemical_list: D004247:DNA
keywords: Baltic Sea; COI; High-throughput sequencing; Nonindigenous species