title: Overexpression of GbRLK, a putative receptor-like kinase gene, improved cotton tolerance to Verticillium wilt.
abstract: Verticillium dahliae is a causative fungal pathogen and only a few genes have been identified that exhibit critical roles in disease resistance and few has shown positive effects on the resistance to Verticillium wilt in transgenic cotton. We cloned a receptor-like kinase gene (GbRLK) induced by Verticillium dahliae (VD) in the disease-resistant cotton Gossypium barbadense cv. Hai7124. Northern blotting revealed that the GbRLK was induced by VD at 96 h after inoculation. The functional GbRLK is from D subgenome since a single base deletion results in a frameshift or dysfunctional homologue in the A subgenome in tetraploid cotton. To verify the function of GbRLK, we developed the overexpression transgenic GbRLK cotton and Arabidopsis lines, and found that they all showed the higher resistance to Verticillium in the greenhouse and field trial. The results of the expression profile using transgenic and non-transgenic Arabidopsis thaliana revealed that the GbRLK regulated expressions of a series genes associated with biotic and abiotic stresses. Therefore, we propose that the increased resistance to Verticillium dahliae infection in transgnic plants could result from reduction in the damage of water loss and regulation of defense gene expression.
authors.affiliation: National Key Laboratory of Crop Genetics &Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, Jiangsu province, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jun
authors.affiliation: National Key Laboratory of Crop Genetics &Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, Jiangsu province, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: National Key Laboratory of Crop Genetics &Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, Jiangsu province, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Gao
authors.affiliation: National Key Laboratory of Crop Genetics &Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, Jiangsu province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: National Key Laboratory of Crop Genetics &Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, Jiangsu province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Fang
authors.affiliation: National Key Laboratory of Crop Genetics &Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, Jiangsu province, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Chen
authors.affiliation: National Key Laboratory of Crop Genetics &Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, Jiangsu province, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Ning
authors.affiliation: National Key Laboratory of Crop Genetics &Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, Jiangsu province, China.
authors.forename: Tianzi
authors.initials: T
authors.lastname: Chen
authors.affiliation: National Key Laboratory of Crop Genetics &Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, Jiangsu province, China.
authors.forename: Wangzhen
authors.initials: W
authors.lastname: Guo
authors.affiliation: National Key Laboratory of Crop Genetics &Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, Jiangsu province, China.
authors.forename: Tianzhen
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D003681:Dehydration / Q000235:genetics / Q000378:metabolism; D060467:Disease Resistance / Q000235:genetics*; D016368:Frameshift Mutation; D015870:Gene Expression; D020869:Gene Expression Profiling; D003368:Gossypium / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D008958:Models, Molecular; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D010935:Plant Diseases; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013312:Stress, Physiological; D020172:Verticillium / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D014867:Water / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins; D014867:Water; D011494:Protein Kinases