title: Mechanism of One-to-Many Molecular Recognition Accompanying Target-Dependent Structure Formation: For the Tumor Suppressor p53 Protein as an Example.
abstract: The new type of molecular recognition, in which an intrinsically disordered region (IDR) of a protein binds to many different target proteins with target-dependent structure formation, is indispensable to the expression of life phenomena and also implicated in a number of diseases. According to the prevailing view, the physicochemical factors responsible for the binding are also target dependent. Here we consider an IDR of the tumor suppressor p53 protein, p53CTD, as an important example related to carcinogenesis and analyze its binding to four targets accompanying the formation of target-dependent structures (i.e., helix, sheet, and two different coils) using our statistical-mechanical method combined with molecular models for water. We find that all of the seemingly different binding processes are driven by a large gain of the translational, configurational entropy of water in the system. The gain originates from sufficiently high shape complementarity on the atomic level within the p53CTD-target interface. It is also required that the electrostatic complementarity be ensured as much as possible to compensate for the dehydration. Such complementarities are achieved in harmony with the portion of the target to which p53CTD binds, leading to a large diversity of structures of p53CTD formed upon binding: If they are not achievable, the binding does not occur. This finding is made possible only by calculating the changes in thermodynamic quantities upon binding and decomposing them into physically insightful components.
authors.affiliation: Institute of Advanced Energy, Kyoto University , Uji, Kyoto 611-0011, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Institute of Advanced Energy, Kyoto University , Uji, Kyoto 611-0011, Japan.
authors.forename: Hiraku
authors.initials: H
authors.lastname: Oshima
authors.affiliation: Institute of Advanced Energy, Kyoto University , Uji, Kyoto 611-0011, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yasuda
authors.affiliation: Institute of Advanced Energy, Kyoto University , Uji, Kyoto 611-0011, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kinoshita
mesh_terms: D006801:Humans; D008958:Models, Molecular; D015233:Models, Statistical; D011487:Protein Conformation; D013816:Thermodynamics; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D016159:Tumor Suppressor Protein p53; D014867:Water