title: Molecular survey of Tamyb10-1 genes and their association with grain colour and germinability in Chinese wheat and Aegilops tauschii.
abstract: To investigate allelic variation of Myb10-1 genes in Chinese wheat and to examine its association with germination level in wheat, a total of 582 Chinese bread wheat cultivars and 110 Aegilops tauschii accessions were used to identify allelic variations of three Myb10-1 genes. Identification results indicated that there is a novel Tamyb10-B1 allele, designated Tamyb10-B1c, in the five Chinese landraces. The Tamyb10-B1c possibly has a large deletion including Tamyb10-B1 gene. There are three novel Tamyb10-D1 alleles (Aetmyb10-D1c, Aetmyb10-D1d and Aetmyb10-D1e) that were discovered in Aegilops tauschii. Of them, Aetmyb10-D1c allele possessed a 104-bp deletion and this resulted in a frame shift in the open reading frame of the Aetmyb10-D1 gene. AETMYB10-D1d and AETMYB10-D1e proteins possessed three and two different amino acids when compared with TAMYB10-D1b protein, respectively. Association of Tamyb10-1 allelic variation with grain germination level indicated that all five allelic combinations with red grains showed a significantly higher GP (germination percentage) and GI (germination index) values than those of white-grained Tamyb10-A1a/Tamyb10-B1a/Tamyb10-D1a genotype after storing it for one year. Moreover, the Tamyb10-A1b/Tamyb10-B1c/Tamyb10-D1b genotype possesses the significantly highest GP and GI among the six different Tamyb10-1 combinations. This study could provide useful information for wheat breeding programme in terms of grain colour and germination level.
authors.affiliation: National Key Laboratory of Wheat and Maize Crop Science/Collaborative Innovation Center of Henan Grain Crops/Agronomy College, Henan Agricultural University, Zhengzhou 450002, People's Republic of China. chf0088@163.com.
authors.forename: Zhong Dong
authors.initials: ZD
authors.lastname: Dong
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Dang Qun
authors.initials: DQ
authors.lastname: Cui
mesh_terms: D000483:Alleles; D001939:Bread; D017343:Genes, Plant; D005819:Genetic Markers; D018525:Germination / Q000235:genetics*; D010858:Pigmentation / Q000235:genetics*; D006109:Poaceae / Q000235:genetics*; D011110:Polymorphism, Genetic; D012639:Seeds / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
chemical_list: D005819:Genetic Markers