title: Simultaneous determination of aminopyrine and antipyrine in porcine muscle, milk, and eggs using liquid chromatography with tandem mass spectrometry.
abstract: The concentrations of residual aminopyrine and antipyrine in porcine muscle, milk, and egg samples were analyzed using liquid chromatography with tandem mass spectrometry after undergoing a series of sample pretreatment steps. Owing to an ion suppression effect, matrix-matched calibrations were used for analyte quantitation with determination coefficients (R(2) ) ≥ 0.9931. The recovery rates for aminopyrine and antipyrine in various matrices at two spiking levels (5 and 10 ng/g) fell in the range of 60.96-68.87 and 61.87-66.99%, respectively. Meanwhile, the intra- and inter-day precisions (expressed as relative standard deviation) were 1.02-12.95 and 1.71-5.50%, respectively. The method's detection limit (1 ng/g) was very low, thus enabling the detection of low residue levels. The applicability of the developed method was demonstrated with actual market samples and none of the tested analytes was detected in any of the samples.
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Jin-A
authors.initials: JA
authors.lastname: Park
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Seong-Kwan
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Cho
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Soo-Min
authors.initials: SM
authors.lastname: Cho
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Hee
authors.initials: H
authors.lastname: Yi
authors.affiliation: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Shim
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Jin-Suk
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.|Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
authors.forename: A M
authors.initials: AM
authors.lastname: Abd El-Aty
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Ho-Chul
authors.initials: HC
authors.lastname: Shin
mesh_terms: D000632:Aminopyrine / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D000983:Antipyrine / Q000032:analysis* / Q000378:metabolism; D002853:Chromatography, Liquid; D004531:Eggs / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D015394:Molecular Structure; D009132:Muscles / Q000737:chemistry*; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D000632:Aminopyrine; D000983:Antipyrine
keywords: Aminopyrine; Animal products; Antipyrine; Pyrazolone derivatives; Tandem mass spectrometry