title: Exploring the inhibitory characteristics of acid hydrolysates upon butanol fermentation: A toxicological assessment.
abstract: This study aimed to quantitatively evaluate the inhibitor tolerance of butanol-producing bacterium Clostridium acetobutylicum. The inhibitory effect of the inhibitors generated by acid pretreatment of biomass feedstock on butanol fermentation decreased in the order of formic acid>oxalic acid>furfural>5-HMF>Na2SO4. C. acetobutylicum has a small tolerance range for furfural (1.06-2.6g/L) and 5-HMF (1.99-2.3g/L). However, the inhibitory effect of Na2SO4 appears to have a wide range, with a chronic toxicity for C. acetobutylicum. All the results could explain, in quantitative manner, the instability of butanol fermentation with C. acetobutylicum from various acid-pretreated feedstocks caused by the fermentation inhibitors.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Wanqian
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Chemical and Materials Engineering, National I-Lan University, I-Lan 260, Taiwan.
authors.forename: Bor-Yann
authors.initials: BY
authors.lastname: Chen
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chieh-Lun
authors.initials: CL
authors.lastname: Cheng
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Yung-Chung
authors.initials: YC
authors.lastname: Lo
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Shih-Hsin
authors.initials: SH
authors.lastname: Ho
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China; Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Nanqi
authors.initials: N
authors.lastname: Ren
mesh_terms: D018533:Biomass; D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005285:Fermentation; D005561:Formates / Q000378:metabolism / Q000494:pharmacology; D005662:Furaldehyde / Q000378:metabolism / Q000494:pharmacology; D006859:Hydrogen / Q000378:metabolism; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods*; D019815:Oxalic Acid / Q000378:metabolism / Q000494:pharmacology; D013431:Sulfates / Q000494:pharmacology
chemical_list: D000440:Butanols; D005561:Formates; D013431:Sulfates; C030544:formic acid; C012036:sodium sulfate; D006859:Hydrogen; D019815:Oxalic Acid; D005662:Furaldehyde
keywords: Butanol fermentation; Dose response; Probit analysis; Toxicity