title: 20 Years of unc-119 as a transgene marker.
abstract: This fall marks 20 years since the cloning of unc-119 was reported. Despite having a strong phenotype that makes animals somewhat difficult to grow and handle, unc-119 mutant rescue has become one of the most frequently-used markers for C. elegans transformation. In this Commentary, I describe the history of how unc-119 rescue traveled through the worm community, contributing to the development of transgene methods in C. elegans.
authors.affiliation: Biology Department; University of California, Riverside ; Riverside, CA USA.
authors.forename: Morris F
authors.initials: MF
authors.lastname: Maduro
keywords: C. elegans; CRISPR/Cas9; MosDEL; MosSCI; miniMos; transformation; transgenesis; unc-119