title: Targeted Mutagenesis in Zebrafish by TALENs.
abstract: Zebrafish is a valuable model organism to study vertebrate development, organ regeneration and to generate human disease models. As an important member of the arsenal of genome editing, TALE nucleases (TALENs) have implicated in broad applications in zebrafish reverse genetic studies. In this chapter, we describe the detailed protocols of TALEN-mediated genome manipulations in zebrafish, including targeted gene disruption by indel mutations, deletion of large genomic regions by using two pairs of TALENs, and precise genome modification by homologous recombination (HR).
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing, 100871, P. R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Huang
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing, 100871, P. R. China.
authors.forename: An
authors.initials: A
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing, 100871, P. R. China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Tong
authors.affiliation: Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
authors.forename: Shuo
authors.initials: S
authors.lastname: Lin
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing, 100871, P. R. China. bzhang@pku.edu.cn.|College of Life Sciences, Peking University, No 5 YiHeYuan Rd., Haidian District, Beijing, 100871, P. R. China. bzhang@pku.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D023281:Genomics; D006801:Humans; D017393:RNA Editing / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*; D015027:Zebrafish / Q000235:genetics*
chemical_list: D015534:Trans-Activators; D004720:Endonucleases
keywords: Chromosomal deletion; Gene disruption; Gene targeting; Genome manipulation; Homologous recombination; Reverse genetics; TALE nuclease; TALEN; Targeted mutagenesis; Zebrafish