title: Comparison of a high-performance liquid chromatography method for quantification of carbamazepine with chemiluminescent microparticle immunoassay.
abstract: Carbamazepine is an antiepileptic drug widely used for the treatment of epilepsy. In the National Institute of Neurology, monitoring has been performed using the technique chemiluminescent microparticle immunoassay (CMIA) in an automated way during the last five years. The aim of this study was to develop a simple and rapid HPLC analytical method coupled to DAD-UV detection for the determination of plasma concentrations of carbamazepine and compare its feasibility with those used in routine analysis. The developed HPLC method was fully validated and the applicability of the proposed method was verified through the analysis of plasma samples of patients and later compared with the quantification of the same plasma samples with the CMIA method. The limit of quantification obtained was 0.5 μg/mL. The mean value for recovery was 99.05% and the coefficient of variation (CV) was 5.6%. The precision and accuracy of this method were within the acceptable limits; inter- and intraday CV values were <10%. The correlation between the CMIA method and the developed HPLC method was very good (r ≈ 0.999). A Bland-Altman plot showed no significant bias between the results. The HPLC-DAD method may be an alternative to determine and monitoring the carbamazepine levels in human plasma or serum. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Facultad de Química, Universidad Nacional Autónoma de México, México D.F., Mexico.
authors.forename: Óscar
authors.initials: Ó
authors.lastname: Guerrero Garduño
authors.affiliation: Instituto Nacional de Neurología y Neurocirugía, México D.F., Mexico.
authors.forename: Dinora F
authors.initials: DF
authors.lastname: González-Esquivel
authors.affiliation: Instituto Nacional de Neurología y Neurocirugía, México D.F., Mexico.
authors.forename: Carmen
authors.initials: C
authors.lastname: Escalante-Membrillo
authors.affiliation: Instituto Nacional de Neurología y Neurocirugía, México D.F., Mexico.
authors.forename: Ángeles
authors.initials: Á
authors.lastname: Fernández
authors.affiliation: Instituto Nacional de Neurología y Neurocirugía, México D.F., Mexico.
authors.forename: Irma Susana
authors.initials: IS
authors.lastname: Rojas-Tomé
authors.affiliation: Instituto Nacional de Neurología y Neurocirugía, México D.F., Mexico.|Facultad de Química, Universidad Nacional Autónoma de México, México D.F., Mexico.
authors.forename: Helgi
authors.initials: H
authors.lastname: Jung Cook
authors.affiliation: Instituto Nacional de Neurología y Neurocirugía, México D.F., Mexico.|Facultad de Química, Universidad Nacional Autónoma de México, México D.F., Mexico.
authors.forename: Nelly
authors.initials: N
authors.lastname: Castro
mesh_terms: D000927:Anticonvulsants / Q000097:blood*; D002220:Carbamazepine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D049449:Luminescence; D015203:Reproducibility of Results
chemical_list: D000927:Anticonvulsants; D002220:Carbamazepine
keywords: CMIA; HPLC-DAD; carbamazepine; methods; praziquantel; validation