title: Acacetin 7-O-α-l-rhamnopyranosyl (1-2) β-D-xylopyranoside Elicits Life-span Extension and Stress Resistance in Caenorhabditis elegans.
abstract: The advancements in the field of gerontology have unraveled the signaling pathways that regulate life span, suggesting that it might be feasible to modulate aging. To this end, we isolated a novel phytomolecule Acacetin 7-O-α-l-rhamnopyranosyl (1-2) β-D-xylopyranoside (ARX) from Premna integrifolia and evaluated its antiaging effects in Caenorhabditis elegans The spectral data analysis revealed the occurrence of a new compound ARX. Out of the three tested pharmacological doses of ARX, viz. 5, 25, and 50 µM, the 25-µM dose was able to extend life span in C. elegans by more than 39%. The present study suggests that ARX affects bacterial metabolism, which in turn leads to dietary restriction (DR)-like effects in the worms. The effect of ARX on worms with mutations (mev-1, eat-2, sir-2.1, skn-1, daf-16, and hsf-1) indicates that ARX-mediated life-span extension involves mechanisms associated with DR and maintenance of cellular redox homeostasis. This study is the first time report on longevity-promoting activity of ARX in C. elegans mediated by stress and DR-regulating genes. This novel phytomolecule can contribute in designing therapeutics for managing aging and age-related diseases.
authors.affiliation: Department of Microbial Technology and Nematology and.
authors.forename: Jyotsna
authors.initials: J
authors.lastname: Asthana
authors.affiliation: Analytical Chemistry Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
authors.forename: Deepti
authors.initials: D
authors.lastname: Yadav
authors.affiliation: Department of Microbial Technology and Nematology and.
authors.forename: Aakanksha
authors.initials: A
authors.lastname: Pant
authors.affiliation: Analytical Chemistry Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Yadav
authors.affiliation: Analytical Chemistry Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
authors.forename: M M
authors.initials: MM
authors.lastname: Gupta
authors.affiliation: Department of Microbial Technology and Nematology and r.pandey@cimap.res.in.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Pandey
mesh_terms: D000375:Aging; D000818:Animals; D030801:Animals, Genetically Modified; D049628:Body Size / Q000187:drug effects; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D005247:Feeding Behavior / Q000187:drug effects; D047309:Flavones / Q000737:chemistry / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008136:Longevity / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D029742:Caenorhabditis elegans Proteins; D047309:Flavones; C023717:acacetin
keywords: Aging; Caenorhabditis elegans; Longevity.; Oxidative stress; Phytomolecule