title: Binding of inferred germline precursors of broadly neutralizing HIV-1 antibodies to native-like envelope trimers.
abstract: HIV-1 envelope glycoproteins (Env) and Env-based immunogens usually do not interact efficiently with the inferred germline precursors of known broadly neutralizing antibodies (bNAbs). This deficiency may be one reason why Env and Env-based immunogens are not efficient at inducing bNAbs. We evaluated the binding of 15 inferred germline precursors of bNAbs directed to different epitope clusters to three soluble native-like SOSIP.664 Env trimers. We found that native-like SOSIP.664 trimers bind to some inferred germline precursors of bNAbs, particularly ones involving the V1/V2 loops at the apex of the trimer. The data imply that native-like SOSIP.664 trimers will be an appropriate platform for structure-guided design improvements intended to create immunogens able to target the germline precursors of bNAbs.
authors.affiliation: Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands. Electronic address: k.h.sliepen@amc.uva.nl.
authors.forename: Kwinten
authors.initials: K
authors.lastname: Sliepen
authors.affiliation: Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands. Electronic address: j.m.medinaramirez@amc.uva.nl.
authors.forename: Max
authors.initials: M
authors.lastname: Medina-Ramírez
authors.affiliation: Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10065, USA. Electronic address: yasmeenanila1@gmail.com.
authors.forename: Anila
authors.initials: A
authors.lastname: Yasmeen
authors.affiliation: Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10065, USA. Electronic address: pek2003@med.cornell.edu.
authors.forename: John P
authors.initials: JP
authors.lastname: Moore
authors.affiliation: Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10065, USA. Electronic address: jpm2003@med.cornell.edu.
authors.forename: Per Johan
authors.initials: PJ
authors.lastname: Klasse
authors.affiliation: Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands; Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10065, USA. Electronic address: r.w.sanders@amc.uva.nl.
authors.forename: Rogier W
authors.initials: RW
authors.lastname: Sanders
mesh_terms: D057134:Antibodies, Neutralizing / Q000276:immunology*; D015483:HIV Antibodies / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D054299:env Gene Products, Human Immunodeficiency Virus / Q000737:chemistry / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D015483:HIV Antibodies; D054299:env Gene Products, Human Immunodeficiency Virus
keywords: Broadly neutralizing antibodies; Env; Germline; HIV-1; SOSIP; Vaccine