title: Gene expression profiling of DMU-212-induced apoptosis and anti-angiogenesis in vascular endothelial cells.
abstract: BACKGROUND
  trans-3,4,5,4'-Tetramethoxystilbene (DMU-212), an derivative of resveratrol, shows strong antiproliferative activities against many cancer cells. In our previous study, we demonstrated that DMU-212 possesses potent proapoptosis and antiangiogenesis effects on vascular endothelial cells (VECs), which made it a promising agent for the treatment of angiogenesis-related diseases.
  
  
  OBJECTIVE
  We studied the gene expression profile of DMU-212-treated VECs to gain further insight into the mechanisms by which DMU-212 exerts its potent pro-apoptosis and antiangiogenesis effects.
  
  
  METHODS
  The potential changes in the gene expression of VECs incubated with DMU-212 were identified and analyzed using the Affymetrix HG-U133 Plus 1.0 array. In addition, the gene expression profile was validated by quantitative real-time PCR (qRT-PCR) analysis for seven of those altered genes.
  
  
  CONCLUSIONS
  DMU-212 was found to regulate a diverse range of genes, including cytokines (IL8, selectin E, MPZL2, EGR1, CCL20, ITGB8, CXCL1, VCAM1, KITLG, and AREG), transport proteins (TRPC4, SLC41A2, SLC17A5, and CREB5), metabolism (CYP1B1, CYP1A1, PDK4, CSNK1G1, MVK, TCEB3C, and CDKN3), enzymes (RAB23, SPHK1, CHSY3, PLAU, PLA2G4C, and MMP10), and genes involved in signal transduction (TMEM217, DUSP8, and SPRY4), chromosome organization (HIST1H2BH and GEM), cell migration and angiogenesis (ERRFI1, HBEGF, and NEDD9), and apoptosis (TNFSF15, TNFRSF9, CD274, BCL2L11, BIRC3, TNFAIP3, and TIFA), as well as other genes with unknown function (PGM5P2, SNORD1142, LOC151760, KRTAP5-2, C1orf110, SNORA14A, MIR31, C2CD4B, SCARNA4, C2orf66, SC4MOL, LOC644714, and LOC283392). This is the first application of microarray technique to investigate and analyze the profile of genes regulated by DMU-212 in VECs. Our results lead to an increased understanding of the signaling pathways involved in DMU-212-induced apoptosis and antiangiogenesis.
authors.affiliation: a College of Bioengineering, Henan University of Technology , Zhengzhou , China.
authors.forename: YiMing
authors.initials: Y
authors.lastname: Miao
authors.affiliation: a College of Bioengineering, Henan University of Technology , Zhengzhou , China.
authors.forename: LiuQing
authors.initials: L
authors.lastname: Cui
authors.affiliation: a College of Bioengineering, Henan University of Technology , Zhengzhou , China.
authors.forename: ZhiQiang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: a College of Bioengineering, Henan University of Technology , Zhengzhou , China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D020869:Gene Expression Profiling / Q000379:methods*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: C482492:3,4,5,4'-tetramethoxystilbene; D020533:Angiogenesis Inhibitors; D013267:Stilbenes
keywords: Angiogenesis; microarray; trans-3,4,5,4′-tetramethoxystilbene