title: Biotinylation and Purification of Surface-exposedHelicobacter pyloriProteins.
abstract: Interactions between pathogenic bacteria and host cells are often mediated by proteins found on the surfaces of the bacteria. The Gram-negative bacteriumHelicobacter pyloriis predicted to produce at least 50 surface-exposed outer membrane proteins, but there has been relatively little progress in experimentally analyzing the cell-surface proteome of this organism. Herein, we describe in detail a protocol that allows biotinylation and purification of surface-exposedH. pyloriproteins. A comparative analysis of surface-exposed proteins identified by this biotinylation-based approach and by several other independent methods is described in a recent publication (Vosset al., 2014).
authors.affiliation: Department of Pathology, Microbiology and Immunology, Nashville, TN.
authors.forename: Bradley J
authors.initials: BJ
authors.lastname: Voss
authors.affiliation: Department of Pathology, Microbiology and Immunology, Nashville, TN ; Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN ; Veterans Affairs Tennessee Valley Healthcare System, Nashville, TN.
authors.forename: Timothy L
authors.initials: TL
authors.lastname: Cover