title: A Survey of the Impact of Deyolking on Biological Processes Covered by Shotgun Proteomic Analyses of Zebrafish Embryos.
abstract: Deyolking, the removal of the most abundant protein from the zebrafish (Danio rerio) embryo, is a common technique for in-depth exploration of proteome-level changes in vivo due to various environmental stressors or pharmacological impacts during embryonic stage of development. However, the effect of this procedure on the remaining proteome has not been fully studied. Here, we report a label-free shotgun proteomics survey on proteome coverage and biological processes that are enriched and depleted as a result of deyolking. Enriched proteins are involved in cellular energetics and development pathways, specifically implicating enrichment related to mitochondrial function. Although few proteins were removed completely by deyolking, depleted molecular pathways were associated with calcium signaling and signaling events implicating immune system response.
authors.affiliation: 1 Department of Pharmacology and Neuroscience, University of North Texas Health Science Center , Fort Worth, Texas.
authors.forename: Fatima
authors.initials: F
authors.lastname: Rahlouni
authors.affiliation: 1 Department of Pharmacology and Neuroscience, University of North Texas Health Science Center , Fort Worth, Texas.
authors.forename: Szabolcs
authors.initials: S
authors.lastname: Szarka
authors.affiliation: 2 Department of Biological Sciences, University of North Texas , Denton, Texas.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Shulaev
authors.affiliation: 1 Department of Pharmacology and Neuroscience, University of North Texas Health Science Center , Fort Worth, Texas.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Prokai
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D016208:Databases, Factual; D004530:Egg Yolk / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction / Q000502:physiology; D053719:Tandem Mass Spectrometry; D059467:Transcriptome; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029961:Zebrafish Proteins