title: Multiple Genetic Mutations Associated with Polymyxin Resistance in Acinetobacter baumannii.
abstract: We studied polymyxin B resistance in 10 pairs of clinical Acinetobacter baumannii isolates, two of which had developed polymyxin B resistance in vivo. All polymyxin B-resistant isolates had lower growth rates than and substitution mutations in the lpx or pmrB gene compared to their parent isolates. There were significant differences in terms of antibiotic susceptibility and genetic determinants of resistance in A. baumannii isolates that had developed polymyxin B resistance in vivo compared to isolates that had developed polymyxin B resistance in vitro.
authors.affiliation: Yong Loo Lin School of Medicine, National University Health System, Singapore Singapore General Hospital, Singapore.
authors.forename: Tze Peng
authors.initials: TP
authors.lastname: Lim
authors.affiliation: Saw Swee Hock School of Public Health, National University Health System, Singapore.
authors.forename: Rick Twee-Hee
authors.initials: RT
authors.lastname: Ong
authors.affiliation: Yong Loo Lin School of Medicine, National University Health System, Singapore.
authors.forename: Pei-Yun
authors.initials: PY
authors.lastname: Hon
authors.affiliation: The University of Melbourne, Melbourne, Australia.
authors.forename: Jane
authors.initials: J
authors.lastname: Hawkey
authors.affiliation: The University of Melbourne, Melbourne, Australia.
authors.forename: Kathryn E
authors.identifier: http://orcid.org/0000-0003-3949-2471
authors.initials: KE
authors.lastname: Holt
authors.affiliation: Singapore General Hospital, Singapore.
authors.forename: Tse Hsien
authors.initials: TH
authors.lastname: Koh
authors.affiliation: Yong Loo Lin School of Medicine, National University Health System, Singapore.
authors.forename: Micky Lo-Ngah
authors.initials: ML
authors.lastname: Leong
authors.affiliation: Singapore General Hospital, Singapore.
authors.forename: Jocelyn Qi-Min
authors.initials: JQ
authors.lastname: Teo
authors.affiliation: Changi General Hospital, Singapore.
authors.forename: Thean Yen
authors.initials: TY
authors.lastname: Tan
authors.affiliation: Yong Loo Lin School of Medicine, National University Health System, Singapore.
authors.forename: Mary Mah-Lee
authors.initials: MM
authors.lastname: Ng
authors.affiliation: Yong Loo Lin School of Medicine, National University Health System, Singapore Saw Swee Hock School of Public Health, National University Health System, Singapore liyang_hsu@yahoo.com.
authors.forename: Li Yang
authors.initials: LY
authors.lastname: Hsu
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D058977:Molecular Sequence Annotation; D009154:Mutation; D011112:Polymyxin B / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D007527:Isoenzymes; C409039:PmrB protein, bacteria; D014157:Transcription Factors; D047090:beta-Lactams; D000217:Acyltransferases; D011112:Polymyxin B