title: miRNA-mediated auxin signalling repression during Vat-mediated aphid resistance in Cucumis melo.
abstract: Resistance to Aphis gossypii in melon is attributed to the presence of the single dominant R gene virus aphid transmission (Vat), which is biologically expressed as antibiosis, antixenosis and tolerance. However, the mechanism of resistance is poorly understood at the molecular level. Aphid-induced transcriptional changes, including differentially expressed miRNA profiles that correspond to resistance interaction have been reported in melon. The potential regulatory roles of miRNAs in Vat-mediated aphid resistance were further revealed by identifying the specific miRNA degradation targets. A total of 70 miRNA:target pairs, including 28 novel miRNA:target pairs, for the differentially expressed miRNAs were identified: 11 were associated with phytohormone regulation, including six miRNAs that potentially regulate auxin interactions. A model for a redundant regulatory system of miRNA-mediated auxin insensitivity is proposed that incorporates auxin perception, auxin modification and auxin-regulated transcription. Chemically inhibiting the transport inhibitor response-1 (TIR-1) auxin receptor in susceptible melon tissues provides in vivo support for the model of auxin-mediated impacts on A. gossypii resistance.
authors.affiliation: College of Agricultural Sciences, The Pennsylvania State University, University Park, PA, 16802, USA.
authors.forename: Sampurna
authors.initials: S
authors.lastname: Sattar
authors.affiliation: Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, 47907, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Addo-Quaye
authors.affiliation: College of Agricultural Sciences, The Pennsylvania State University, University Park, PA, 16802, USA.
authors.forename: Gary A
authors.initials: GA
authors.lastname: Thompson
mesh_terms: D000818:Animals; D001042:Aphids; D028463:Cucumis melo / Q000378:metabolism* / Q000502:physiology; D007210:Indoleacetic Acids / Q000378:metabolism*; D035683:MicroRNAs / Q000502:physiology*; D010937:Plant Growth Regulators / Q000378:metabolism / Q000502:physiology*; D010940:Plant Proteins / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D007210:Indoleacetic Acids; D035683:MicroRNAs; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C046131:auxin receptor, plant
keywords: Aphis gossypii; Auxin; Cucumis melo; Vat; miRNA