title: HPLC Plasma Assay of a Novel Anti-MRSA Compound, Kaempferol-3-O-Alpha-L-(2",3"-di-p-coumaroyl)rhamnoside, from Sycamore Leaves.
abstract: Methicillin-resistant Staphylococcus aureus (MRSA) is a serious pathogen that is resistant to current antibiotic therapy. Thus, there is an urgent need for novel antimicrobial agents that can effectively combat these new strains of drug-resistant "superbugs". Recently, fractionation of an extract from Platanus occidentalis (American sycamore) leaves produced an active kaempferol molecule, 3-O-alpha-L-(2",3"-di-p-coumaroyl)rhamnoside (KCR), in four isomeric forms; all four isomers exhibit potent anti-MRSA activity. In order to further the preclinical development of KCR as a new antibiotic class, we developed and validated a simple analytical method for assaying KCR plasma concentration. Because KCR will be developed as a new drug, although comprising four stereoisomers, the analytical method was devised to assay the total amount of all four isomers. In the present work, both a plasma processing procedure and an HPLC method have been developed and validated. Mouse plasma containing KCR was first treated with ethanol and then centrifuged. The supernatant was dried, suspended in ethanol, centrifuged, and the supernatant was injected into an HPLC system comprising a Waters C18, a mobile phase composing methanol, acetonitrile, and trifluoroacetic acid and monitored at 313 nm. The method was validated by parameters including a good linear correlation, a limit of quantification of 0.27 microg/mL, and high accuracy. In summary, this method allows a rapid analysis of KCR in the plasma samples for pharmacokinetics studies.
authors.forename: Yiguan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Frederick
authors.initials: F
authors.lastname: Valeriote
authors.forename: Kenneth
authors.initials: K
authors.lastname: Swartz
authors.forename: Ben
authors.initials: B
authors.lastname: Chen
authors.forename: Mark T
authors.initials: MT
authors.lastname: Hamann
authors.forename: Douglas L
authors.initials: DL
authors.lastname: Rodenburg
authors.forename: James D
authors.initials: JD
authors.lastname: McChesney
authors.forename: Jiajiu
authors.initials: J
authors.lastname: Shaw
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D044949:Kaempferols / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D019684:Magnoliopsida / Q000737:chemistry; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D051379:Mice; D008826:Microbial Sensitivity Tests; D018515:Plant Leaves / Q000737:chemistry; D013203:Staphylococcal Infections / Q000097:blood* / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D044949:Kaempferols; C540136:kaempferol-3-O-alpha-L-(2''-E-p-coumaroyl-4''-Z-p-coumaroyl)-rhamnoside