title: Comparative evaluation of the hydrogen production by mixed consortium, synthetic co-culture and pure culture using distillery effluent.
abstract: Wastewater comprises of various carbon sources. So, the use of microbial consortium may improve the hydrogen production and organic reduction. The present study deals with biohydrogen production by acidogenic mixed consortia (AMC), synthetic co-culture (Klebsiella pneumoniae IIT-BT 08 and Citrobacter freundii IIT-BT L139) and pure culture using distillery effluent (DE). Higher hydrogen yield was observed in case of AMC (9.17 mol/kg CODreduced) as compared to the synthetic co-culture and pure culture. PCR-DGGE analysis indicated that the consortium was predominated by species closely affiliated to Clostridium sp. The average hydrogen production rate was 267 mL/Lh. The maximum hydrogen production rate (Rm), hydrogen production potential (P) and lag time (λ) by AMC using DE were 507.2 mL/Lh, 3729 m/L and 2.04 h, respectively. Maximum gaseous energy recovery by AMC was found to be higher by 21.9% and 45.4% than that of using co-culture and pure culture respectively.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Mishra
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal, India.
authors.forename: Shantonu
authors.initials: S
authors.lastname: Roy
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal, India. Electronic address: ddas.iitkgp@gmail.com.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Das
mesh_terms: D056804:Biofuels; D002244:Carbon / Q000378:metabolism; D016971:Citrobacter freundii / Q000378:metabolism; D003013:Clostridium / Q000378:metabolism; D018920:Coculture Techniques / Q000379:methods*; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods; D006859:Hydrogen / Q000378:metabolism*; D007220:Industrial Waste; D007711:Klebsiella pneumoniae / Q000378:metabolism; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater
chemical_list: D056804:Biofuels; D002244:Carbon; D006859:Hydrogen; D007220:Industrial Waste; D062065:Wastewater
keywords: Acidogenic mixed consortium; Biohydrogen; Distillery effluent; Klebsiella pneumoniae IIT-BT 08; Synthetic co-culture