title: Characterization of miRNAs from hydrothermal vent shrimp Rimicaris exoculata.
abstract: Deep-sea hydrothermal vent shrimp Rimicaris exoculata is a dominant species aggregating in vent fields along the Mid-Atlantic Ocean Ridge. MicroRNAs play important roles in life cycles of eukaryotes. However, little is known about miRNAs of vent animals. In the present study, a small RNA cDNA library from the muscle of R. exoculata was constructed and the miRNA sequencing was performed. The results indicated that a total of 7,983,331 raw reads were obtained, representing 569,354 unique sequences. Based on sequence analysis, R. exoculata contained 159 conserved miRNAs and 34 novel miRNAs. The conserved miRNAs included 54 families belonging to three different taxonomic units (bilaterian, protostomes and arthropods). The results also showed that miR-2001, a lost miRNA in crustaceans, existed in R. exoculata. Among the conserved miRNAs, iso-miRs were detected. Therefore, this study presented the first insight into the miRNAs of deep-sea hydrothermal vent animals.
authors.affiliation: Collaborative Innovation Center of Deep Sea Biology and College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China; Key Laboratory of Marine Ecosystem and Biogeochemistry, The Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Collaborative Innovation Center of Deep Sea Biology and College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Yaodong
authors.initials: Y
authors.lastname: He
authors.affiliation: Key Laboratory of Marine Ecosystem and Biogeochemistry, The Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Collaborative Innovation Center of Deep Sea Biology and College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China. Electronic address: zxb0812@zju.edu.cn.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017124:Conserved Sequence; D033364:Decapoda / Q000235:genetics*; D060086:Hydrothermal Vents; D035683:MicroRNAs / Q000235:genetics*; D010802:Phylogeny
chemical_list: D035683:MicroRNAs
keywords: Rimicaris exoculata; miRNA