title: DIARRHEA OUTBREAK IN PERNAMBUCO, BRAZIL, ASSOCIATED WITH A HEAT-STABLE CYTOTOXIC ENTEROTOXIN PRODUCED BY Aeromonas caviae.
abstract: In the present study enterotoxic and cytotoxic activities of twenty Aeromonas caviae strains were examined. They originated from fecal specimens of patients with acute diarrhea during an outbreak in Brazil in 2004. Culture supernatants of fourteen strains (70%) caused fluid accumulation in rabbit ileal intestinal loops and in suckling mice assays, and also showed a cytotoxic activity in Vero and Caco-2 cells. The enterotoxic and cytotoxic factors were heat-stable after culture supernatants treatment at 100 ºC. The results revealed that A. caviae strains produce a putative diarrheagenic virulence factor, a heat-stable cytotoxic enterotoxin that could be linked to the diarrhea outbreak that took place in Brazil.
authors.affiliation: Evolution and Bioagents Department, State University of Campinas, Campinas, SP, BR.
authors.forename: Ana Carolina Amaral
authors.initials: AC
authors.lastname: Lopes
authors.affiliation: Enteric and Special Pathogens Infectious Diseases Department, Instituto Adolfo Lutz, Sao Paulo, SP, BR.
authors.forename: Luciano Moura
authors.initials: LM
authors.lastname: Martins
authors.affiliation: Evolution and Bioagents Department, State University of Campinas, Campinas, SP, BR.
authors.forename: Maria Silvia Viccari
authors.initials: MS
authors.lastname: Gatti
authors.affiliation: Bacteriology Department, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, BR.
authors.forename: Cristhiane Moura
authors.initials: CM
authors.lastname: Falavina Dos Reis
authors.affiliation: Bacteriology Department, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, BR.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Hofer
authors.affiliation: Evolution and Bioagents Department, State University of Campinas, Campinas, SP, BR.
authors.forename: Tomomasa
authors.initials: T
authors.lastname: Yano
mesh_terms: D057585:Aeromonas caviae / Q000472:pathogenicity*; D000818:Animals; D001938:Brazil; D002460:Cell Line; D003967:Diarrhea / Q000382:microbiology; D004196:Disease Outbreaks; D004768:Enterotoxins / Q000096:biosynthesis*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D037521:Virulence Factors / Q000096:biosynthesis*
chemical_list: D004768:Enterotoxins; D037521:Virulence Factors