title: AMBRA1 and SQSTM1 expression pattern in prostate cancer.
abstract: Prostate cancer is among the most commonly diagnosed male diseases and a leading cause of cancer mortality in men. There is emerging evidence that autophagy plays an important role in malignant cell survival and offers protection from the anti-cancer drugs in prostate cancer cells. AMBRA1 and the autophagic protein sequestosome-1 (SQSTM1; p62) expression were evaluated by immunohistochemistry and western blot on tissue samples from both benign and malignant prostatic lesions. The data reported in this pilot study demonstrated an increased expression of AMBRA1 and SQSTM1, which were also associated with an accumulation of LC3II in prostate cancer but not in benign lesion. In the present study we found that: (i) at variance with benign lesion, prostate cancer cells underwent SQSTM1 accumulation, i.e., clearly displayed a defective autophagic process but, also, (ii) prostate cancer accumulated AMBRA1 and (iii) this increase positively correlated with the Gleason score. These results underscore a possible implication of autophagy in prostate cancer phenotype and of AMBRA1 as possible cancer progression biomarker in this malignancy.
authors.affiliation: Laboratory of Cell Biology and Electron Microscopy, National Institute for Infectious Diseases I.R.C.C.S. 'L. Spallanzani', Rome, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Falasca
authors.affiliation: Department of Systems Medicine, Chair of Medical Oncology, Tor Vergata University of Rome, Rome, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Torino
authors.affiliation: Department of Drug Research and Medicine Evaluation, Istituto Superiore di Sanita', Rome, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Marconi
authors.affiliation: Department of Urology, Regina Elena National Cancer Institute, IRCCS, Rome, Italy.|Laboratory of Molecular Medicine and Biotechnology, University Campus Bio-Medico of Rome, Rome, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Costantini
authors.affiliation: Department of Urology, Regina Elena National Cancer Institute, IRCCS, Rome, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Pompeo
authors.affiliation: Department of Pathology, Regina Elena National Cancer, Institute, IRCCS, Rome, Italy.
authors.forename: Steno
authors.initials: S
authors.lastname: Sentinelli
authors.affiliation: Department of Pathology, Regina Elena National Cancer, Institute, IRCCS, Rome, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: De Salvo
authors.affiliation: Department of Drug Research and Medicine Evaluation, Istituto Superiore di Sanita', Rome, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Patrizio
authors.affiliation: Life Line Lab S.R.L., Guidonia, RM, Italy.
authors.forename: Cristiano
authors.initials: C
authors.lastname: Padula
authors.affiliation: Department of Urology, Regina Elena National Cancer Institute, IRCCS, Rome, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Gallucci
authors.affiliation: Laboratory of Cell Biology and Electron Microscopy, National Institute for Infectious Diseases I.R.C.C.S. 'L. Spallanzani', Rome, Italy.|Department of Biology, University of Rome 'Tor Vergata', Rome, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Piacentini
authors.affiliation: Department of Drug Research and Medicine Evaluation, Istituto Superiore di Sanita', Rome, Italy. valter.malorni@iss.it.|Istituto San Raffaele Pisana, Rome, Italy. valter.malorni@iss.it.
authors.forename: Walter
authors.initials: W
authors.lastname: Malorni
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000368:Aged; D000970:Antineoplastic Agents / Q000627:therapeutic use; D001343:Autophagy / Q000502:physiology; D014408:Biomarkers, Tumor / Q000378:metabolism; D002470:Cell Survival / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011467:Prostate / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D012189:Retrospective Studies; D000071456:Sequestosome-1 Protein
chemical_list: C553334:AMBRA1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; C099718:SQSTM1 protein, human; D000071456:Sequestosome-1 Protein
keywords: Autophagy; Electron microscopy; Immunohistochemistry; Prostate cancer