title: Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root.
abstract: Methods for staining tissues with Oil Red O and hematoxylin-eosin are classical histological techniques that are widely used to quantify atherosclerotic burden in mouse tissues because of their ease of use, reliability, and the large amount of information they provide. These stains can provide quantitative data about the impact of a genetic or environmental factor on atherosclerotic burden and on the initiation, progression, or regression of the disease, and can also be used to evaluate the efficacy of drugs designed to prevent or treat atherosclerosis. This chapter provides protocols for quantifying atherosclerotic burden in mouse aorta and aortic root, including methods for dissection, Oil Red O staining, hematoxylin-eosin staining, and image analysis.
authors.affiliation: Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Vascular Biology Program, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029, Madrid, Spain.
authors.forename: M Jesús
authors.initials: MJ
authors.lastname: Andrés-Manzano
authors.affiliation: Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Vascular Biology Program, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029, Madrid, Spain. vandres@cnic.es.
authors.forename: Vicente
authors.initials: V
authors.lastname: Andrés
authors.affiliation: Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Vascular Biology Program, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029, Madrid, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Dorado
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology*; D001018:Aortic Diseases / Q000473:pathology*; D050197:Atherosclerosis / Q000473:pathology*; D001391:Azo Compounds; D004396:Coloring Agents; D004195:Disease Models, Animal; D004210:Dissection; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D007090:Image Interpretation, Computer-Assisted; D051379:Mice; D008853:Microscopy; D016612:Paraffin Embedding; D058226:Plaque, Atherosclerotic; D012720:Severity of Illness Index; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; C011049:oil red O; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin
keywords: Aorta; Aortic root; Atherosclerosis; Dissection; Hematoxylin-eosin; Oil Red O