title: Radiation signature on exposed cells: Relevance in dose estimation.
abstract: The radiation is considered as a double edged sword, as its beneficial and detrimental effects have been demonstrated. The potential benefits are being exploited to its maximum by adopting safe handling of radionuclide stipulated by the regulatory agencies. While the occupational workers are monitored by personnel monitoring devices, for general publics, it is not a regular practice. However, it can be achieved by using biomarkers with a potential for the radiation triage and medical management. An ideal biomarker to adopt in those situations should be rapid, specific, sensitive, reproducible, and able to categorize the nature of exposure and could provide a reliable dose estimation irrespective of the time of the exposures. Since cytogenetic markers shown to have many advantages relatively than other markers, the origins of various chromosomal abnormalities induced by ionizing radiations along with dose-response curves generated in the laboratory are presented. Current status of the gold standard dicentric chromosome assay, micronucleus assay, translocation measurement by fluorescence in-situ hybridization and an emerging protein marker the γ-H2AX assay are discussed with our laboratory data. With the wide choice of methods, an appropriate assay can be employed based on the net.
authors.affiliation: Venkatachalam Perumal, Tamizh Selvan Gnana Sekaran, Venkateswarlu Raavi, Safa Abdul Syed Basheerudeen, Karthik Kanagaraj, Amith Roy Chowdhury, Solomon FD Paul, Department of Human Genetics, College of Biomedical Sciences, Technology and Research, Sri Ramachandra University, Porur, Chennai 600 116, India.
authors.forename: Venkatachalam
authors.initials: V
authors.lastname: Perumal
authors.affiliation: Venkatachalam Perumal, Tamizh Selvan Gnana Sekaran, Venkateswarlu Raavi, Safa Abdul Syed Basheerudeen, Karthik Kanagaraj, Amith Roy Chowdhury, Solomon FD Paul, Department of Human Genetics, College of Biomedical Sciences, Technology and Research, Sri Ramachandra University, Porur, Chennai 600 116, India.
authors.forename: Tamizh Selvan
authors.initials: TS
authors.lastname: Gnana Sekaran
authors.affiliation: Venkatachalam Perumal, Tamizh Selvan Gnana Sekaran, Venkateswarlu Raavi, Safa Abdul Syed Basheerudeen, Karthik Kanagaraj, Amith Roy Chowdhury, Solomon FD Paul, Department of Human Genetics, College of Biomedical Sciences, Technology and Research, Sri Ramachandra University, Porur, Chennai 600 116, India.
authors.forename: Venkateswarlu
authors.initials: V
authors.lastname: Raavi
authors.affiliation: Venkatachalam Perumal, Tamizh Selvan Gnana Sekaran, Venkateswarlu Raavi, Safa Abdul Syed Basheerudeen, Karthik Kanagaraj, Amith Roy Chowdhury, Solomon FD Paul, Department of Human Genetics, College of Biomedical Sciences, Technology and Research, Sri Ramachandra University, Porur, Chennai 600 116, India.
authors.forename: Safa Abdul Syed
authors.initials: SA
authors.lastname: Basheerudeen
authors.affiliation: Venkatachalam Perumal, Tamizh Selvan Gnana Sekaran, Venkateswarlu Raavi, Safa Abdul Syed Basheerudeen, Karthik Kanagaraj, Amith Roy Chowdhury, Solomon FD Paul, Department of Human Genetics, College of Biomedical Sciences, Technology and Research, Sri Ramachandra University, Porur, Chennai 600 116, India.
authors.forename: Karthik
authors.initials: K
authors.lastname: Kanagaraj
authors.affiliation: Venkatachalam Perumal, Tamizh Selvan Gnana Sekaran, Venkateswarlu Raavi, Safa Abdul Syed Basheerudeen, Karthik Kanagaraj, Amith Roy Chowdhury, Solomon FD Paul, Department of Human Genetics, College of Biomedical Sciences, Technology and Research, Sri Ramachandra University, Porur, Chennai 600 116, India.
authors.forename: Amith Roy
authors.initials: AR
authors.lastname: Chowdhury
authors.affiliation: Venkatachalam Perumal, Tamizh Selvan Gnana Sekaran, Venkateswarlu Raavi, Safa Abdul Syed Basheerudeen, Karthik Kanagaraj, Amith Roy Chowdhury, Solomon FD Paul, Department of Human Genetics, College of Biomedical Sciences, Technology and Research, Sri Ramachandra University, Porur, Chennai 600 116, India.
authors.forename: Solomon Fd
authors.initials: SF
authors.lastname: Paul
keywords: Biomarker; Dicentric chromosomes; Fluorescence in-situ hybridization; Micronucleus