title: Genetic analysis of 15 mtDNA SNP loci in Chinese Yi ethnic group using SNaPshot minisequencing.
abstract: SNaPshot minisequencing is a rapid and robust methodology based on a single base extension with a labeled ddNTP. The present study detected 15 selected SNPs in the mitochondrial DNA (mtDNA) control and coding regions by minisequencing methodology using SNaPshot for forensic purpose. The samples were collected from 99 unrelated individuals of the Yi ethnic minority group in Yunnan Province. We have predominantly found high-frequency transitions (91.7%) and a significantly lower frequency of transversions (8.3%). The nt152, 489, 8701, 10,398, 16,183, and 16,362 loci were highly polymorphic, while the nt231, 473 and 581 loci were not polymorphic in the studied population. Based on these 15 SNPs, a total of 28 mtDNA haplotypes were defined in 99 individuals with the haplotype diversity of 0.9136. Also, we compared the mtDNA sequences of Yi group and other 9 populations worldwide and drew a Neighbor-Joining tree based on the shared 12 mtDNA SNP loci, which demonstrated a close relationship between Yi and Bai groups. In conclusion, the analysis of the 15 selected SNPs increases considerably the discrimination power of mtDNA. Moreover, the SNaPshot minisequencing method could quickly detect mtDNA SNPs, and is economical and sensitive. The set of selected 15 SNPs is highly informative and is capable for anthropology genetic analysis.
authors.affiliation: Neurology Department of Cadre's Ward, 2nd Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi Province, PR China.
authors.forename: Chun-Ting
authors.initials: CT
authors.lastname: Hu
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Science, Beijing, PR China.
authors.forename: Jiang-Wei
authors.initials: JW
authors.lastname: Yan
authors.affiliation: Department of Forensic Medicine, Nanjing Medical University, Nanjing, Jiangsu, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Forensic Science Service of the Beijing Public Security Bureau, Beijing, PR China.
authors.forename: Qing-Xia
authors.initials: QX
authors.lastname: Zhang
authors.affiliation: Medical Genetics Institute of Henan Province, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, Henan, PR China.
authors.forename: Hong-Dan
authors.initials: HD
authors.lastname: Wang
authors.affiliation: Department of Forensic Medicine, Nanjing Medical University, Nanjing, Jiangsu, PR China.
authors.forename: Cai-Yong
authors.initials: CY
authors.lastname: Yin
authors.affiliation: Department of Forensic Medicine, Nanjing Medical University, Nanjing, Jiangsu, PR China.
authors.forename: Han-Ting
authors.initials: HT
authors.lastname: Fan
authors.affiliation: Department of Forensic Medicine, Nanjing Medical University, Nanjing, Jiangsu, PR China.
authors.forename: Ling-Li
authors.initials: LL
authors.lastname: Hu
authors.affiliation: Blood Center of the Shaanxi Province, Shaanxi, PR China.
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Shen
authors.affiliation: Research Center of Stomatology, Stomatological Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi Province, PR China.
authors.forename: Hao-Tian
authors.initials: HT
authors.lastname: Meng
authors.affiliation: Research Center of Stomatology, Stomatological Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi Province, PR China.
authors.forename: Yu-Dang
authors.initials: YD
authors.lastname: Zhang
authors.affiliation: Neurology Department of Cadre's Ward, 2nd Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi Province, PR China. Electronic address: wanghuixjtu@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Research Center of Stomatology, Stomatological Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi Province, PR China.
authors.forename: Bo-Feng
authors.initials: BF
authors.lastname: Zhu
mesh_terms: D044466:Asian People; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D056426:Genetic Loci; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004272:DNA, Mitochondrial
keywords: Genetic distance; Mitochondrial DNA; SNaPshot; Single nucleotide polymorphism; Yi ethnic group