title: Treatment of keloid scars with a 1210-nm diode laser in an animal model.
abstract: OBJECTIVE
  A temperature increase can improve wound healing by activation of heat shock protein 70 and stimulation of fibroblasts. Since keloids are a dysfunction of collagen fiber synthesis and organization, this study aimed to evaluate if a 1,210 nm diode laser could have effects in a new animal model of keloid scars.
  
  
  METHODS
  A total of 39 nude mice were used for this study. Phototypes IV and V human keloids were grafted into their backs and after 1 month of healing, the mice were divided into four groups: Control, Laser, Resection, Resection/Laser. In the Laser group, the keloids were treated with a 1,210-nm diode-laser with the following parameters: 4 W; 10 seconds; fluence: 51 J/cm(2) ; spot: 18.9 × 3.7 mm(2) . In the Resection group, surgical intra-lesional excision was performed. In the Resection/Laser group, keloids were treated with the 1,210-nm laser-diode after surgical intra-lesional excision. Temperature measurements were made during the laser treatment. Clinical examination and histological study were performed on the day of treatment and 1 month, 2 months, and 3 months later.
  
  
  RESULTS
  Mean temperature measurement was of 44.8°C (42-48°) in the Laser groups. No healing complications or keloid proliferation was observed in any group. Keloid histologic characters were confirmed in all grafts. No histologic particularity was observed in the laser groups in comparison with the Control and Resection groups.
  
  
  CONCLUSIONS
  First, this keloid animal model appears to be adapted for laser study. Secondly, the 1,210-nm diode laser does not induce keloid thermal damage in vivo. Further studies with different 1,210-nm laser diode parameters should be performed in order to observe significant effects on keloids.
authors.affiliation: Department of Plastic Surgery, AP-HM, Marseille, 13915, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Philandrianos
authors.affiliation: Department of Plastic Surgery, AP-HM, Marseille, 13915, France.|Aix-Marseille University, Marseille, France.
authors.forename: Baptiste
authors.initials: B
authors.lastname: Bertrand
authors.affiliation: Aix-Marseille University, Marseille, France.|Department of Pathology, AP-HM, Marseille, 13915, France.
authors.forename: Lucile
authors.initials: L
authors.lastname: Andrac-Meyer
authors.affiliation: Department of Plastic Surgery, AP-HM, Marseille, 13915, France.
authors.forename: Guy
authors.initials: G
authors.lastname: Magalon
authors.affiliation: Department of Plastic Surgery, AP-HM, Marseille, 13915, France.|Aix-Marseille University, Marseille, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Casanova
authors.affiliation: Department of Plastic Surgery, CHU Brest, Brest, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Kerfant
authors.affiliation: INSERM U1189 ONCO-THAI, Lille, 59037, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Mordon
mesh_terms: D000818:Animals; D005260:Female; D006801:Humans; D007627:Keloid / Q000473:pathology / Q000601:surgery*; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D051379:Mice; D008819:Mice, Nude; D016896:Treatment Outcome; D014945:Wound Healing
keywords: animal model; keloid; laser diode; thermal laser; wound healing