title: Bone Morphogenetic Protein-7 Inhibits EMT-Associated Genes in Breast Cancer.
abstract: OBJECTIVE
  Bone morphogenetic protein-7 (BMP7) has been shown to reduce the severity of injury-induced fibrosis through counteracting the fibrotic effects of transforming growth factor β 1 (TGFβ1). However, this model in the carcinogenesis of breast cancer is unknown.
  
  
  METHODS
  We analyzed the effects of BMP7 and TGFβ1 on gene transcripts and protein levels of EMT-related factors in breast cancer cells by RT-qPCR and Western blot, respectively. The effects of BMP7 and TGFβ1 on cell invasiveness and migration were evaluated by scratch wound healing assay and transwell cell migration assay. The cell growth was measured by MTT assay.
  
  
  RESULTS
  BMP7 did not alter the TGFβ1-stimulated phosphorylation of TGFβ receptor, but significantly inhibited the TGFβ1-activated epithelial-mesenchymal transition (EMT)-related genes in breast cancer cells, resulting in a significant reduction in TGFβ1-triggered cell growth and cell metastasis.
  
  
  CONCLUSIONS
  Our data suggest that besides being a well-known antagonist for TGFβ1 in fibrosis, BMP7 may also antagonize TGFβ1 in tumorigenesis-associated EMT in breast cancer. Thus, BMP7 may be a promising therapeutic target for treating breast cancer.
authors.forename: Xuexiang
authors.initials: X
authors.lastname: Ying
authors.forename: Yunpo
authors.initials: Y
authors.lastname: Sun
authors.forename: Pingqing
authors.initials: P
authors.lastname: He
mesh_terms: D055419:Bone Morphogenetic Protein 7 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000235:genetics / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D010766:Phosphorylation; D060888:Real-Time Polymerase Chain Reaction; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology
chemical_list: D055419:Bone Morphogenetic Protein 7; D015820:Cadherins; D018125:Receptors, Transforming Growth Factor beta; D011994:Recombinant Proteins; D053773:Transforming Growth Factor beta1; D052005:Bone Morphogenetic Protein Receptors, Type I