title: Long-term manure amendments reduced soil aggregate stability via redistribution of the glomalin-related soil protein in macroaggregates.
abstract: Glomalin-related soil protein (GRSP) contributes to the formation and maintenance of soil aggregates, it is however remains unclear whether long-term intensive manure amendments alter soil aggregates stability and whether GRSP regulates these changes. Based on a three-decade long fertilization experiment in northeast China, this study examined the impact of long-term manure input on soil organic carbon (SOC), total and easily extractable GRSP (GRSPt and GRSPe) and their respective allocations in four soil aggregates (>2000 μm; 2000-250 μm; 250-53 μm; and <53 μm). The treatments include no fertilization (CK), low and high manure amendment (M1, M2), chemical nitrogen, phosphorus and potassium fertilizers (NPK), and combined manure and chemical fertilizers (NPKM1, NPKM2). Though SOC, GRSPe and GRSPt in soil and SOC in each aggregate generally increased with increasing manure input, GRSPt and GRSPe in each aggregate showed varying changes with manure input. Both GRSP in macroaggregates (2000-250 μm) were significantly higher under low manure input, a pattern consistent with changes in soil aggregate stability. Constituting 38~49% of soil mass, macroaggregates likely contributed to the nonlinear changes of aggregate stability under manure amendments. The regulatory process of GRSP allocations in soil aggregates has important implications for manure management under intensive agriculture.
authors.affiliation: State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110164, China.
authors.forename: Hongtu
authors.initials: H
authors.lastname: Xie
authors.affiliation: Department of Agriculture and Environmental Sciences, Tennessee State University, Nashville, TN 37209, USA.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Land &Environment, Shenyang Agricultural University, Shenyang, 110866, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: College of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian, 116028, China.
authors.forename: Lianfeng
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Land &Environment, Shenyang Agricultural University, Shenyang, 110866, China.
authors.forename: Jingkuan
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110164, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: He
authors.affiliation: State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110164, China.|National Field Research Station of Agroecosystems, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000383:Agriculture; D005308:Fertilizers / Q000032:analysis; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry*; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry*; D008372:Manure / Q000032:analysis / Q000382:microbiology*; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D005308:Fertilizers; D005656:Fungal Proteins; D006023:Glycoproteins; D008372:Manure; D012987:Soil; C573268:glomalin