title: The effective bioengineering method of implantation decellularized renal extracellular matrix scaffolds.
abstract: End stage renal disease (ESRD) is a progressive loss of kidney function with a high rate of morbidity and mortality. Transplantable organs are hard to come by and hold a high risk of recipient immune rejection. We intended to establish a more effective and faster method to decellularize and recellularize the kidney scaffold for transplant and regeneration. We successfully produced renal scaffolds by decellularizing rat kidneys with 0.5% sodium dodecyl sulfate (SDS), while still preserving the extracellular matrix (ECM) 3D architecture, an intact vascular tree and biochemical components. We recellularized the kidney scaffolds with mouse embryonic stem (ES) cells that then populated and proliferated within the glomerular, vascular, and tubular structures. After in vivo implantation, these recellularized scaffolds were easily reperfused, tolerated blood pressure and produced urine with no blood leakage. Our methods can successfully decellularize and recellularize rat kidneys to produce functional renal ECM scaffolds. These scaffolds maintain their basic components, retain intact vasculature and show promise for kidney regeneration.
authors.affiliation: Department of Urology, The Second Hospital, Shandong University, Shandong, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Department of Kidney Transplantation, The Second Hospital, Shandong University, Shandong, China.
authors.forename: Shuangde
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Central Research Lab, The Second Hospital, Shandong University, Shandong, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Central Research Lab, The Second Hospital, Shandong University, Shandong, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Cheng
authors.affiliation: Department of Central Research Lab, The Second Hospital, Shandong University, Shandong, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Kong
authors.affiliation: Department of Central Research Lab, The Second Hospital, Shandong University, Shandong, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Luan
authors.affiliation: Department of Urology, The Second Hospital, Shandong University, Shandong, China.
authors.forename: Xiaoshuai
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Urology, The Second Hospital, Shandong University, Shandong, China.
authors.forename: Shengtian
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Urology, The Second Hospital, Shandong University, Shandong, China.
authors.forename: Denglu
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Central Research Lab, The Second Hospital, Shandong University, Shandong, China.
authors.forename: Jue
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Central Research Lab, The Second Hospital, Shandong University, Shandong, China.
authors.forename: Kailin
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Urology, The Second Hospital, Shandong University, Shandong, China.
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D057005:Bioengineering / Q000379:methods*; D005109:Extracellular Matrix / Q000502:physiology*; D007668:Kidney / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D054457:Tissue Scaffolds
keywords: decellularization; kidney regeneration; recellularization; scaffold; tissue engineering