title: Mitigation of copper toxicity by DNA oligomers in green paramecia.
abstract: Impact of transition metals which catalyze the generation of reactive oxygen species (ROS), on activation of cell death signaling in plant cells have been documented to date. Similarly in green paramecia (Paramecium bursaria), an aquatic protozoan species harboring symbiotic green algae in the cytoplasm, toxicities of various metallic ions have been documented. We have recently examined the effects of double-stranded GC-rich DNA fragments with copper-binding nature and ROS removal catalytic activity as novel plant cell-protecting agents, using the suspension-cultured tobacco cells. Here, we show that above DNA oligomers protect the cells of green paramecia from copper-induced cell death, suggesting that the phenomenon firstly observed in tobacco cells is not limited only within higher plants but it could be universally observable in wider range of organisms.
authors.affiliation: a Laboratory of Chemical Biology and Bioengineering; Faculty and Graduate School of Environmental Engineering; The University of Kitakyushu ; Kitakyushu , Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takaichi
authors.affiliation: a Laboratory of Chemical Biology and Bioengineering; Faculty and Graduate School of Environmental Engineering; The University of Kitakyushu ; Kitakyushu , Japan.|b University of Florence; LINV Kitakyushu Research Center (LINV@Kitakyushu) ; Kitakyushu , Japan.
authors.forename: Diego
authors.initials: D
authors.lastname: Comparini
authors.affiliation: b University of Florence; LINV Kitakyushu Research Center (LINV@Kitakyushu) ; Kitakyushu , Japan.|c Collaboration center; Kyushu Institute of Technology ; Kitakyushu , Japan.
authors.forename: Junichiro
authors.initials: J
authors.lastname: Iwase
authors.affiliation: b University of Florence; LINV Kitakyushu Research Center (LINV@Kitakyushu) ; Kitakyushu , Japan.|d Université Paris Diderot; Sorbonne Paris Cité; Institut des Energies de Demain (FRE 3597) ; Paris , France.|e LINV-DiSPAA ; Department of Agri-Food and Environmental Science ; University of Florence ; Sesto Fiorentino (FI) , Italy.
authors.forename: François
authors.initials: F
authors.lastname: Bouteau
authors.affiliation: b University of Florence; LINV Kitakyushu Research Center (LINV@Kitakyushu) ; Kitakyushu , Japan.|e LINV-DiSPAA ; Department of Agri-Food and Environmental Science ; University of Florence ; Sesto Fiorentino (FI) , Italy.|f Université Paris Diderot; Sorbonne Paris Cité; Paris 7 Interdisciplinary Energy Research Institute (PIERI) ; Paris , France.
authors.forename: Stefano
authors.initials: S
authors.lastname: Mancuso
authors.affiliation: a Laboratory of Chemical Biology and Bioengineering; Faculty and Graduate School of Environmental Engineering; The University of Kitakyushu ; Kitakyushu , Japan.|b University of Florence; LINV Kitakyushu Research Center (LINV@Kitakyushu) ; Kitakyushu , Japan.|f Université Paris Diderot; Sorbonne Paris Cité; Paris 7 Interdisciplinary Energy Research Institute (PIERI) ; Paris , France.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kawano
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D016923:Cell Death / Q000187:drug effects; D003300:Copper / Q000633:toxicity*; D019610:Cytoprotection / Q000187:drug effects; D004247:DNA / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010247:Paramecium / Q000166:cytology / Q000187:drug effects*
chemical_list: D003300:Copper; D004247:DNA
keywords: DNA oligomers; ROS; cell death; green algae; metal toxicity