title: Measuring Cell-Cell Binding Using Flow-Cytometry.
abstract: Cell-cell adhesion mediates a number of competitive and cooperative microbial interactions. Fluorescence labeling and flow cytometry techniques allow us to observe and measure these interactions rapidly and easily. Here, we describe a method to quantify cell-cell adhesion events between two differentially labeled cell populations.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA, 93106, USA.
authors.forename: Zachary C
authors.initials: ZC
authors.lastname: Ruhe
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA, 93106, USA.|Biomolecular Science and Engineering Program, University of California, Santa Barbara, CA, 93106, USA.
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Hayes
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA, 93106, USA. low@lifesci.ucsb.edu.|Biomolecular Science and Engineering Program, University of California, Santa Barbara, CA, 93106, USA. low@lifesci.ucsb.edu.
authors.forename: David A
authors.initials: DA
authors.lastname: Low
mesh_terms: D002448:Cell Adhesion; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes
keywords: CdiA; Contact-dependent growth inhibition; Flow cytometry; Fluorescent proteins; Self/nonself recognition; cell–cell binding