title: Methods for TALEN Evaluation, Use, and Mutation Detection in the Mosquito Aedes aegypti.
abstract: The generation and study of transgenic Aedes aegypti mosquitoes provides an essential tool for elucidating the complex molecular biology of this important vector. Within the field, genetic manipulation has surpassed the proof of principle stage and is now utilized in both applied and theoretical vector control strategies. The application of new instruments, technologies and techniques allows ever more controlled experiments to be conducted. In this text we describe microinjection of Ae. aegypti embryos in the context of evaluating and performing genomic editing with transcription activator-like effector nucleases (TALENs).
authors.affiliation: 305 Fralin Life Science Institute, Virginia Tech, West Campus Dr., Blacksburg, VA, 24061, USA.|Department of Entomology, Virginia Tech, Blacksburg, VA, 20461, USA.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Basu
authors.affiliation: 305 Fralin Life Science Institute, Virginia Tech, West Campus Dr., Blacksburg, VA, 24061, USA.|Department of Entomology, Virginia Tech, Blacksburg, VA, 20461, USA.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Aryan
authors.affiliation: 305 Fralin Life Science Institute, Virginia Tech, West Campus Dr., Blacksburg, VA, 24061, USA.|Department of Entomology, Virginia Tech, Blacksburg, VA, 20461, USA.
authors.forename: Mary Etna
authors.initials: ME
authors.lastname: Haac
authors.affiliation: 305 Fralin Life Science Institute, Virginia Tech, West Campus Dr., Blacksburg, VA, 24061, USA.|Department of Entomology, Virginia Tech, Blacksburg, VA, 20461, USA.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Myles
authors.affiliation: 305 Fralin Life Science Institute, Virginia Tech, West Campus Dr., Blacksburg, VA, 24061, USA. zachadel@vt.edu.|Department of Entomology, Virginia Tech, Blacksburg, VA, 20461, USA. zachadel@vt.edu.
authors.forename: Zach N
authors.initials: ZN
authors.lastname: Adelman
mesh_terms: D000330:Aedes / Q000235:genetics* / Q000821:virology; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods*; D003715:Dengue / Q000235:genetics* / Q000635:transmission / Q000821:virology; D003716:Dengue Virus / Q000472:pathogenicity; D004720:Endonucleases / Q000235:genetics*; D016678:Genome; D007303:Insect Vectors / Q000235:genetics / Q000821:virology; D009154:Mutation; D017393:RNA Editing / Q000235:genetics
chemical_list: D004720:Endonucleases
keywords: Aedes; Gene editing; HRMA; Mosquito; Mutagenesis; Nuclease; TALEN