title: Proteomics analysis of liver tissues from C57BL/6J mice receiving low-dose 137Cs radiation.
abstract: Differentially expressed proteins in liver tissues of C57BL/6J mice receiving low-dose (137)Cs radiation were examined by proteomics analysis. Compared with the control group, 80 proteins were differentially expressed in the irradiated group. Among the 40 randomly selected proteins used for peptide mass fingerprinting analysis and bioinformatics, 24 were meaningful. These proteins were related to antioxidant defense, amino acid metabolism, detoxification, anti-tumor development, amino acid transport, anti-peroxidation, and composition of respiratory chain. Western blot analysis showed that catalase (CAT), glycine N-methyltransferase (GNMT), and glutathione S-transferase P1 (GSTP1) were up-regulated in the irradiated group; these results were in agreement with qPCR results. These results show that CAT, GNMT, and GSTP1 may be related to stress response induced by low-dose irradiation in mice liver. The underlying mechanism however requires further investigation.
authors.affiliation: Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, Hunan, 421001, People's Republic of China.|College of Pharmacy and Biological Sciences, University of South China, Hengyang, Hunan, 421001, People's Republic of China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yi
authors.affiliation: College of Pharmacy and Biological Sciences, University of South China, Hengyang, Hunan, 421001, People's Republic of China.
authors.forename: Linwei
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, Hunan, 421001, People's Republic of China.|College of Pharmacy and Biological Sciences, University of South China, Hengyang, Hunan, 421001, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yin
authors.affiliation: Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, Hunan, 421001, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Hu
authors.affiliation: Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, Hunan, 421001, People's Republic of China.
authors.forename: Guangyue
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, Hunan, 421001, People's Republic of China. dingdxzzz@163.com.
authors.forename: Dexin
authors.initials: D
authors.lastname: Ding
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D050938:Glycine N-Methyltransferase / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000378:metabolism; D040901:Proteomics
chemical_list: D000975:Antioxidants; D011506:Proteins; D002374:Catalase; D050938:Glycine N-Methyltransferase; D005982:Glutathione Transferase
keywords: C57BL/6EJ mice; CAT; GNMT; GSTP1; Low-dose irradiation; Proteomics