title: Genome Editing in Mice Using TALE Nucleases.
abstract: Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.
authors.affiliation: Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764, Neuherberg, Germany. benedikt.wefers@helmholtz-muenchen.de.|Deutsches Zentrum für Neurodegenerative Erkrankungen e. V. (DZNE), 81377, Munich, Germany. benedikt.wefers@helmholtz-muenchen.de.
authors.forename: Benedikt
authors.initials: B
authors.lastname: Wefers
authors.affiliation: Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764, Neuherberg, Germany. Christina.brandl@helmholtz-muenchen.de.|Technische Universität München, 85350, Freising-Weihenstephan, Germany. Christina.brandl@helmholtz-muenchen.de.
authors.forename: Christina
authors.initials: C
authors.lastname: Brandl
authors.affiliation: Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764, Neuherberg, Germany. oskar.ortiz@helmholtz-muenchen.de.
authors.forename: Oskar
authors.initials: O
authors.lastname: Ortiz
authors.affiliation: Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764, Neuherberg, Germany. wurst@helmholtz-muenchen.de.|Deutsches Zentrum für Neurodegenerative Erkrankungen e. V. (DZNE), 81377, Munich, Germany. wurst@helmholtz-muenchen.de.|Technische Universität München, 85350, Freising-Weihenstephan, Germany. wurst@helmholtz-muenchen.de.|Max Planck Institute of Psychiatry, 80804, Munich, Germany. wurst@helmholtz-muenchen.de.|Munich Cluster for Systems Neurology (SyNergy), Ludwig-Maximilians-Universität München, 81377, Munich, Germany. wurst@helmholtz-muenchen.de.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wurst
authors.affiliation: Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764, Neuherberg, Germany. ralf.kuehn@mdc-berlin.de.|Technische Universität München, 85350, Freising-Weihenstephan, Germany. ralf.kuehn@mdc-berlin.de.|Max-Delbrück-Center for Molecular Medicine, 13125, Berlin, Germany. ralf.kuehn@mdc-berlin.de.|Berlin Institute of Health, Berlin, Germany. ralf.kuehn@mdc-berlin.de.
authors.forename: Ralf
authors.initials: R
authors.lastname: Kühn
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D053903:DNA Breaks, Double-Stranded; D004720:Endonucleases / Q000235:genetics*; D055786:Gene Knockout Techniques / Q000379:methods*; D016678:Genome; D051379:Mice; D008845:Microinjections; D009154:Mutation; D017393:RNA Editing / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D004720:Endonucleases
keywords: Gene targeting; Knock-in; Knockout; Mouse; One-cell embryo; TALEN