title: DNA oxidation and DNA repair in gills of zebra mussels exposed to cadmium and benzo(a)pyrene.
abstract: Freshwater bivalve molluscs are considered as effective indicators of environmental pollution. The comet assay allows the detection of DNA damage such as DNA strand breaks and alkali-labile sites. The main oxidative lesion, 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), which is a pre-mutagenic lesion, can be detected by the comet assay coupled with the hOGG1 DNA repair enzyme. With this modified assay we recently observed that BaP induced 8-oxodG lesions and with the modified comet-Fpg assay we observed that Cd induced oxidative DNA damage. The aim of this study was to determine the stability of DNA lesions in Cd and BaP exposed zebra mussels using the comet-hOGG1 assay. Mussels were exposed for 24 h to these two chemicals and then placed in clean water for 6 days. We observed that BaP (7, 12 and 18 µg/L) induced an increase of DNA strand break levels as soon as 6 h of exposure and that the two highest concentrations of BaP induced a low level of hOGG1-sensitive sites. After 2 days of depuration, BaP induced DNA lesions returned to the basal level, indicating an effective DNA repair. Cd (3, 32 and 81 µg/L) induced an increase of the DNA strand break levels and a low level of hOGG1-sensitive sites. This study revealed that BaP-induced DNA lesions are repaired more efficiently than Cd-induced DNA lesions. As the level of hOGG1 sensitive sites was increased in Cd and BaP exposed mussels, it seems that these chemicals induce 8-oxo-dG.
authors.affiliation: Unité de Recherches Hydrosystèmes et Bioprocédés, IRSTEA, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761, Antony Cedex, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Michel
authors.affiliation: Unité de Recherches Hydrosystèmes et Bioprocédés, IRSTEA, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761, Antony Cedex, France. fvincent@ifremer.fr.|Laboratory of Microbiology-LNR, IFREMER, rue de l'île d'Yeu, BP 21105, 44311, Nantes Cedex 03, France. fvincent@ifremer.fr.
authors.forename: Françoise
authors.initials: F
authors.lastname: Vincent-Hubert
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002104:Cadmium / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage; D004260:DNA Repair; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D049875:Dreissena / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D005880:Gills / Q000378:metabolism; D010084:Oxidation-Reduction; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D002104:Cadmium; D001564:Benzo(a)pyrene; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-oxodG; Comet-hOGG1 assay; DNA repair; Oxidative stress; Zebra mussel