title: Choices for Induction of Pluripotency: Recent Developments in Human Induced Pluripotent Stem Cell Reprogramming Strategies.
abstract: The ability to generate human induced pluripotent stem cells (iPSCs) from somatic cells provides tremendous promises for regenerative medicine and its use has widely increased over recent years. However, reprogramming efficiencies remain low and chromosomal instability and tumorigenic potential are concerns in the use of iPSCs, especially in clinical settings. Therefore, reprogramming methods have been under development to generate safer iPSCs with higher efficiency and better quality. Developments have mainly focused on the somatic cell source, the cocktail of reprogramming factors, the delivery method used to introduce reprogramming factors and culture conditions to maintain the generated iPSCs. This review discusses the developments on these topics and briefly discusses pros and cons of iPSCs in comparison with human embryonic stem cells generated from somatic cell nuclear transfer.
authors.affiliation: Department of Cognitive Neuroscience, Radboudumc, Nijmegen, 6500, HB, The Netherlands.
authors.forename: Marinka
authors.initials: M
authors.lastname: Brouwer
authors.affiliation: Department of Human Genetics, Radboudumc, Nijmegen, 6500, HB, The Netherlands. jo.zhou@radboudumc.nl.|Department of Molecular Developmental Biology, Faculty of Science, Radboud University, Nijmegen, 6500, HB, The Netherlands. jo.zhou@radboudumc.nl.
authors.forename: Huiqing
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Cognitive Neuroscience, Radboudumc, Nijmegen, 6500, HB, The Netherlands. n.nadif@donders.ru.nl.|Department of Human Genetics, Radboudumc, Nijmegen, 6500, HB, The Netherlands. n.nadif@donders.ru.nl.|Donders Institute for Brain, Cognition, and Behaviour , Centre for Neuroscience, Nijmegen, 6525, AJ, The Netherlands. n.nadif@donders.ru.nl.
authors.forename: Nael
authors.initials: N
authors.lastname: Nadif Kasri
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D044968:Regenerative Medicine / Q000379:methods* / Q000639:trends; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014780:Viruses / Q000235:genetics
chemical_list: D015415:Biomarkers; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C489427:MYC protein, human; D035683:MicroRNAs; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D016271:Proto-Oncogene Proteins c-myc; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors
keywords: Human induced pluripotent stem cells; Reprogramming