title: Mixed Substrate Fermentation for Enhanced Phytase Production by Thermophilic Mould Sporotrichum thermophile and Its Application in Beneficiation of Poultry Feed.
abstract: The optimum values of the critical variables determined by the central composite design of response surface methodology (RSM) for maximum phytase production (1881.26 U g(-1) dry mouldy residue (DMR)) by Sporotrichum thermophile are 2.5 % Tween 80, 1.0 % yeast extract and 48 h of incubation period. Phytase production in the mixed substrate (sugarcane bagasse and wheat bran) fermentation enhanced 11.6-fold over the initial production as a consequence of optimization. Phytase titres are sustainable in flasks, trays and column bioreactor (1796 to 2095 U g(-1) DMR), thus validating the model and the process for large-scale phytase production. When the yeast extract was replaced with corn steep liquor (2 % w/v), a sustained enzyme titre (1890 U g(-1) DMR) was attained, making the process cost-effective. Among all the detergents, Tween 80 supported a higher phytase production than others. The enzyme efficiently liberated nutritional components from poultry feed (inorganic phosphate, soluble protein and reducing sugars) in a time-dependent manner.
authors.affiliation: Laboratory of Bioprocess Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak, 124001, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kumari
authors.affiliation: Department of Microbiology, University of Delhi, South Campus, Benito Juarez Road, New Delhi, 110021, India.
authors.forename: T
authors.initials: T
authors.lastname: Satyanarayana
authors.affiliation: Laboratory of Bioprocess Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak, 124001, India. ohlanbs@gmail.com.
authors.forename: Bijender
authors.initials: B
authors.lastname: Singh
mesh_terms: D000821:Animal Feed; D000818:Animals; D005285:Fermentation; D011200:Poultry; D013175:Sporothrix / Q000378:metabolism*
keywords: Mixed substrate; Phytase; Poultry feed; Solid-state fermentation; Sporotrichum thermophile BJTLR50