title: In vitro assessment of retention and microleakage in pit and fissure sealants following enamel pre-etching with sodium hypochlorite deproteinisation.
abstract: OBJECTIVE
  The purpose of this study was to assess and compare the rate of sealant retention and microleakage after placement on etched enamel with and without prior deproteinisation.
  
  
  METHODS
  
  
  
  METHODS
  75 freshly extracted third molars were randomly assigned to either of two pit and fissure treatment methods. Samples from both groups were etched with 37% phosphoric acid gel for 15 seconds, followed by placement of a sealant, and then subjected to thermocycling for evaluation of sealant retention. After that, specimens were immersed in rhodamine B, sectioned longitudinally, and examined under a confocal laser scanning microscope for assessment of microleakage. Collected data were statistically analysed using chi-square and Fisher exact tests with an α level of 0.05.
  
  
  RESULTS
  The rate of sealant retention was similar between the two study groups (P = 0.073), but the rate of sealant microleakage was significantly lower in the enamel deproteinisation group (P < 0.001) than in the control group.
  
  
  CONCLUSIONS
  Based on these findings, we recommend the deproteinisation method prior to enamel acid etching to obtain better clinical results with sealants.
authors.affiliation: Pediatric Dentistry Postgraduate Program, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, México.
authors.forename: A
authors.initials: A
authors.lastname: Garrocho-Rangel
authors.affiliation: Pediatric Dentistry Postgraduate Program, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, México.
authors.forename: C
authors.initials: C
authors.lastname: Lozano-Vázquez
authors.affiliation: Pediatric Dentistry Postgraduate Program, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, México.
authors.forename: C
authors.initials: C
authors.lastname: Butrón-Tellez-Girón
authors.affiliation: Basic Sciences Laboratory, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, México.
authors.forename: D
authors.initials: D
authors.lastname: Escobar-García
authors.affiliation: Pediatric Dentistry Postgraduate Program, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, México.
authors.forename: S
authors.initials: S
authors.lastname: Ruíz-Rodriguez
authors.affiliation: Pediatric Dentistry Postgraduate Program - Basic Sciences Laboratory. Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, México.
authors.forename: A
authors.initials: A
authors.lastname: Pozos-Guillén
mesh_terms: D000134:Acid Etching, Dental; D006801:Humans; D066298:In Vitro Techniques; D010895:Pit and Fissure Sealants; D011506:Proteins / Q000737:chemistry*; D012973:Sodium Hypochlorite / Q000008:administration & dosage*
chemical_list: D010895:Pit and Fissure Sealants; D011506:Proteins; D012973:Sodium Hypochlorite