title: Immunomodulatory activity on macrophage of a purified polysaccharide extracted from Laminaria japonica.
abstract: In this work, a novel water-soluble homogeneous polysaccharide (LJP-31) with a molecular mass of 2.24 × 10(6) Da was isolated and purified from Laminaria japonica using DEAE-cellulose and Sephacryl S500 chromatography. Results showed that LJP-31 mainly consists of arabinose, mannose, glucose and galactose in a molar ratio of 1.0:7.8:6.6:0.8. LJP-31 exhibited significant stimulation on macrophages and enhanced the production of NO, TNF-α, IL-1β, IL-6 and IL-10 as well as the up-regulation of their gene expressions. Western blot analysis suggested that LJP-31 has the positive effects on the translocation of NF-κB p65 from cytoplasm to nucleus and the phosphorylation of IκBα, ERK1/2, JNK1/2 and P38 in macrophages. Flow cytometric and confocal laser-scanning microscopy analysis indicated that toll-like receptor 4 (TLR4) was at least one of the recognition receptors of LJP-31 on the plasma membrane of macrophages. Taken together, LJP-31 may exert its immunostimulating potency via TLR4 activation of MAPK and NF-κB signaling pathways.
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Fang
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, PR China.
authors.forename: Jing-Fei
authors.initials: JF
authors.lastname: Wang
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, PR China. Electronic address: xueqiang.zha@upr.edu.
authors.forename: Xue-Qiang
authors.initials: XQ
authors.lastname: Zha
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, PR China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Cui
authors.affiliation: Institute of Agro-products Processing, Anhui Academy of Agricultural Sciences, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cao
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, PR China. Electronic address: jianpingluo@hfut.edu.cn.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Luo
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D016753:Interleukin-10 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D007795:Laminaria / Q000737:chemistry*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D016328:NF-kappa B / Q000276:immunology; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D051197:Toll-Like Receptor 4 / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D015850:Interleukin-6; D016328:NF-kappa B; D011134:Polysaccharides; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10
keywords: Immunostimulation; Laminaria japonica; Macrophage; Polysaccharides; TLR4