title: Effects of Sodium Arsenite and Arsenate in Testicular Histomorphometry and Antioxidants Enzymes Activities in Rats.
abstract: The main source of environmental arsenic exposure in most countries of the world is drinking water in which inorganic forms of arsenic predominate. The present study was aimed to test the impact of two different compounds of inorganic arsenic in histomorphometric and enzymatic parameters in the testes by oral exposition. Adult Wistar male rats were exposed to sodium arsenite and arsenate in drinking water, testing for each chemical form the concentrations of 0.01 and 10 mg/L per 56 days. The animals intoxicated with arsenic, mainly sodium arsenite, showed reduction in the percentage of seminiferous epithelium and in proportion and volume of Leydig cells. Moreover, there was an increase in the percentage of tunica propria, lumen, lymphatic space, blood vessels, and macrophages. The activity of superoxide dismutase (SOD) did not change among the groups. However, the activity of catalase (CAT) decreased in animals exposed to both arsenic compounds. In addition, the higher concentration of arsenic, mainly as sodium arsenite, caused vacuolization in the seminiferous epithelium. The body and testes weight as well as testosterone concentration remained unchanged among the groups. In conclusion, exposition to arsenic, mainly as sodium arsenite, caused alteration in histomorphometric parameters and antioxidant defense system in the testes.
authors.affiliation: Department of General Biology, Federal University of Viçosa (UFV), Av. P.H. Rolfs, s/n, Campus Universitário, Viçosa, Minas Gerais, 36570-000, Brazil.
authors.forename: Ana Cláudia Ferreira
authors.initials: ACF
authors.lastname: Souza
authors.affiliation: Department of General Biology, Federal University of Viçosa (UFV), Av. P.H. Rolfs, s/n, Campus Universitário, Viçosa, Minas Gerais, 36570-000, Brazil.
authors.forename: Sarah Cozzer
authors.initials: SC
authors.lastname: Marchesi
authors.affiliation: Department of General Biology, Federal University of Viçosa (UFV), Av. P.H. Rolfs, s/n, Campus Universitário, Viçosa, Minas Gerais, 36570-000, Brazil.
authors.forename: Graziela
authors.initials: G
authors.lastname: Domingues de Almeida Lima
authors.affiliation: Department of General Biology, Federal University of Viçosa (UFV), Av. P.H. Rolfs, s/n, Campus Universitário, Viçosa, Minas Gerais, 36570-000, Brazil.
authors.forename: Rafael Penha
authors.initials: RP
authors.lastname: Ferraz
authors.affiliation: Department of General Biology, Federal University of Viçosa (UFV), Av. P.H. Rolfs, s/n, Campus Universitário, Viçosa, Minas Gerais, 36570-000, Brazil.
authors.forename: Felipe Couto
authors.initials: FC
authors.lastname: Santos
authors.affiliation: Department of General Biology, Federal University of Viçosa (UFV), Av. P.H. Rolfs, s/n, Campus Universitário, Viçosa, Minas Gerais, 36570-000, Brazil.
authors.forename: Sérgio Luis Pinto
authors.initials: SLP
authors.lastname: da Matta
authors.affiliation: Department of General Biology, Federal University of Viçosa (UFV), Av. P.H. Rolfs, s/n, Campus Universitário, Viçosa, Minas Gerais, 36570-000, Brazil. mariana.mneves@ufv.br.
authors.forename: Mariana
authors.initials: M
authors.lastname: Machado-Neves
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001149:Arsenates / Q000008:administration & dosage / Q000494:pharmacology*; D018053:Arsenites / Q000008:administration & dosage / Q000494:pharmacology*; D002374:Catalase / Q000037:antagonists & inhibitors / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017670:Sodium Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology
chemical_list: D000975:Antioxidants; D001149:Arsenates; D018053:Arsenites; D017670:Sodium Compounds; C017947:sodium arsenite; D002374:Catalase; D013482:Superoxide Dismutase; C025657:arsenic acid
keywords: Arsenate; Arsenite; Light microscopy; Morphometry; Oxidative stress; Testes