title: Efficient magnesium lactate production with in situ product removal by crystallization.
abstract: In this paper, attempts were made to develop an in situ product removal process for magnesium lactate production based on crystallization. The crystallization was conducted at 42°C without seed crystal addition. The product concentration, productivity and yield of fermentation coupled with in situ product removal (ISPR) reached 143 g L(-1), 2.41 g L(-1)h(-1) and 94.3%. In four cycles of crystallization, the average reuse rate of fermentation medium and removal rate of product reached 64.0% and 77.7%. At the same time, ISPR fermentation saved 40% water, 41% inorganic salts and 43% yeast extract (YE) as compared to fed-batch fermentation. The process introduces an effective way to reduce the amount of waste water and the raw material cost in magnesium lactate fermentation.
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Di
authors.initials: D
authors.lastname: Cai
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Changjing
authors.initials: C
authors.lastname: Chen
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Peiyong
authors.initials: P
authors.lastname: Qin
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: twtan@mail.buct.edu.cn.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
mesh_terms: D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D003460:Crystallization; D005285:Fermentation; D007773:Lactates / Q000737:chemistry* / Q000302:isolation & purification; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism; D017616:Magnesium Compounds / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D007773:Lactates; D017616:Magnesium Compounds
keywords: Crystallization; Lactic acid fermentation; Lactobacillus rhamnosus; Magnesium lactate