title: Modulation of gene expression in CD4+ T lymphocytes following in vitro HIV infection: a comparison between human and chimpanzee.
abstract: Chimpanzees are susceptible to experimental infection by human deficiency virus (HIV)-1, but unlike humans, they exceptionally develop an immunodeficiency syndrome after HIV-1 inoculation. To explore the difference between human and chimpanzee, we analyzed the expression of 1547 genes of various functions in human or chimpanzee CD4+ lymphoblasts inoculated in vitro with HIV-1. We observed that, 1 day after HIV inoculation, fifty-eight genes were up-regulated in lymphoblasts of the three humans while their expression remained unchanged in lymphoblasts of the three chimpanzees. One gene is involved in adhesion of HIV (catenin-alpha), three in the immune response (semaphorin 4D, placental growth factor, IL-6), three in apoptosis (deleted in colorectal carcinoma, caspase 9 and FOXO1A). No difference between species was revealed for the expression of 373 genes related to glycosylation pathways. The in vitro human/chimpanzee comparison reveals new candidate genes up-regulated after inoculation with HIV-1 only in human lymphoblasts and which could be related to the higher sensitivity of human to HIV-induced AIDS.
authors.affiliation: Laboratoire d'Immunogénétique Moléculaire (EA3034), Faculté de médecine Toulouse- Rangueil, Université Paul Sabatier, Bâtiment A2, 133 Route de Narbonne, 31062 Toulouse Cedex 04, France.|Laboratoire d'Immunologie, CHU de Toulouse, Hôpital Rangueil, 1 Avenue Jean Poulhès, TSA 50032, 31059 Toulouse Cedex 9, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Puissant-Lubrano
authors.affiliation: Laboratoire d'Immunogénétique Moléculaire (EA3034), Faculté de médecine Toulouse- Rangueil, Université Paul Sabatier, Bâtiment A2, 133 Route de Narbonne, 31062 Toulouse Cedex 04, France.|Laboratoire d'Immunologie, CHU de Toulouse, Hôpital Rangueil, 1 Avenue Jean Poulhès, TSA 50032, 31059 Toulouse Cedex 9, France.
authors.forename: Pol-André
authors.initials: PA
authors.lastname: Apoil
authors.affiliation: Laboratoire d'Immunogénétique Moléculaire (EA3034), Faculté de médecine Toulouse- Rangueil, Université Paul Sabatier, Bâtiment A2, 133 Route de Narbonne, 31062 Toulouse Cedex 04, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Gleizes
authors.affiliation: Institut des Sciences de la Vie et de la Santé, Université de Limoges (EA3 176), Limoges, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Forestier
authors.affiliation: Institut des Sciences de la Vie et de la Santé, Université de Limoges (EA3 176), Limoges, France.
authors.forename: Raymond
authors.initials: R
authors.lastname: Julien
authors.affiliation: Université de Toulouse-Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 9, France.
authors.forename: Peter
authors.initials: P
authors.lastname: Winterton
authors.affiliation: Laboratoire de Virologie, CHU de Toulouse, Hôpital Purpan, TSA 40031, 31059 Toulouse Cedex 9, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Pasquier
authors.affiliation: Laboratoire de Virologie, CHU de Toulouse, Hôpital Purpan, TSA 40031, 31059 Toulouse Cedex 9, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Izopet
authors.affiliation: Laboratoire d'Immunogénétique Moléculaire (EA3034), Faculté de médecine Toulouse- Rangueil, Université Paul Sabatier, Bâtiment A2, 133 Route de Narbonne, 31062 Toulouse Cedex 04, France.|Laboratoire d'Immunologie, CHU de Toulouse, Hôpital Rangueil, 1 Avenue Jean Poulhès, TSA 50032, 31059 Toulouse Cedex 9, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Blancher
keywords: Array; CD4+ T lymphocytes; Human; Immune response; Non-human primates; qPCR