title: Contrasting microbial community composition and function perspective in sections of a full-scale coking wastewater treatment system.
abstract: To characterize the microbial community of the coking wastewater (CWW) treatment system and to study the effects of CWW characteristics and operational parameters on microbial communities, active sludge samples were collected from a full-scale CWW treatment plant using three-phase fluidized bed biological reactors. High-throughput MiSeq sequencing was used to examine the 16S rRNA genes of microbiology, revealing a distinct microbial composition among the active sludge samples of three sequential bioreactors. Pseudomonas, Comamonas, and Thiobacillus-related sequences dominated in the anaerobic bioreactor A, aerobic bioreactor O1, and aerobic bioreactor O2 active sludge with relative abundance of 72.59, 56.75, and 27.82 %, respectively. The physico-chemical characteristics of CWW were analyzed by standard methods and operational parameters were recorded to examine their effects on the microbial communities. The redundancy analysis (RDA) results showed that the bacterial communities of bioreactors A, O1, and O2 correlated strongly with cyanides, phenols, and ammonia, respectively. These results expand the knowledge about the biodiversity and population dynamics of microorganisms and discerned the relationships between bacterial communities and environmental variables in the biological treatment processes in the full-scale CWW treatment system.
authors.affiliation: Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, People's Republic of China.|College of Biological Science and Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.|School of Environment and Energy, South China University of Technology, Guangzhou, 510006, People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, People's Republic of China.|College of Biological Science and Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.|School of Environment and Energy, South China University of Technology, Guangzhou, 510006, People's Republic of China.
authors.forename: Haizhen
authors.initials: H
authors.lastname: Wu
authors.affiliation: Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, People's Republic of China. cechwei@scut.edu.cn.|College of Biological Science and Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China. cechwei@scut.edu.cn.|School of Environment and Energy, South China University of Technology, Guangzhou, 510006, People's Republic of China. cechwei@scut.edu.cn.
authors.forename: Chaohai
authors.initials: C
authors.lastname: Wei
authors.affiliation: Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, People's Republic of China.|College of Biological Science and Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.|School of Environment and Energy, South China University of Technology, Guangzhou, 510006, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, People's Republic of China.|College of Biological Science and Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.|School of Environment and Energy, South China University of Technology, Guangzhou, 510006, People's Republic of China.
authors.forename: Junting
authors.initials: J
authors.lastname: Xie
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D044822:Biodiversity; D019149:Bioreactors; D003077:Coke; D003486:Cyanides / Q000378:metabolism; D004269:DNA, Bacterial; D059013:Microbial Consortia / Q000235:genetics* / Q000502:physiology*; D010636:Phenols / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000382:microbiology*
chemical_list: D000641:Ammonia; D003077:Coke; D003486:Cyanides; D004269:DNA, Bacterial; D010636:Phenols; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater
keywords: 16S rDNA; Active sludge; Bacterial community; Coking wastewater; Microbiology