title: Intracerebroventricular Injection of Alarin Increased Glucose Uptake in Skeletal Muscle of Diabetic Rats.
abstract: In order to investigate the central effect of alarin on glucose uptake, we administered alarin and/ or its inhibitor, ala6-25Cys into the cerebral ventricles of the type 2 diabetic rats. Then the relative parameters about glucose uptake in skeletal muscles were measured. We found that central treatment with alarin significantly increased the food intake, body weight and glucose infusion rates in hyperinsulinemic euglycemic clamp tests of the animals. Besides, the treatment also enhanced 2-deoxy-[3H]-D-glucose uptake, vesicle-associated membrane protein 2 contents, glucose transporter 4 protein and mRNA expression, as well as pAktThr308, pAktSer473 and total Akt levels in muscle cells, but reduced plasma glucose and insulin levels of the rats. All of the alarin-inducing events may be antagonised by central injection of ala6-25Cys. These results suggest that central administration of alarin stimulates glucose uptake mediated by activation of Akt signal pathway in type 2 diabetic animals.
authors.affiliation: Department of Endocrinology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu, China, 225001.
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, First People's Hospital, Yangzhou University, Yangzhou, China, 225001.
authors.forename: Yongkang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: School of Medicine, Yangzhuo Polytechnic College, Yangzhou, Jiangsu, China, 225009.
authors.forename: Shudong
authors.initials: S
authors.lastname: Sheng
authors.affiliation: Key Laboratory of Gerontal Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu, China, 225001.
authors.forename: Lili
authors.initials: L
authors.lastname: Guo
authors.affiliation: Key Laboratory of Gerontal Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu, China, 225001.
authors.forename: Biao
authors.initials: B
authors.lastname: He
authors.affiliation: Key Laboratory of Gerontal Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu, China, 225001.
authors.forename: Penghua
authors.initials: P
authors.lastname: Fang
authors.affiliation: Key Laboratory of Gerontal Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu, China, 225001.
authors.forename: Mingyi
authors.initials: M
authors.lastname: Shi
authors.affiliation: Department of Endocrinology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu, China, 225001; Key Laboratory of Gerontal Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu, China, 225001.
authors.forename: Ping
authors.initials: P
authors.lastname: Bo
authors.affiliation: Department of Endocrinology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu, China, 225001.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002460:Cell Line; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004435:Eating / Q000187:drug effects; D044089:Galanin-Like Peptide / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D007276:Injections, Intraventricular; D007328:Insulin / Q000097:blood; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats
chemical_list: D044089:Galanin-Like Peptide; D051275:Glucose Transporter Type 4; D007328:Insulin; C581934:alarin; D051057:Proto-Oncogene Proteins c-akt; D005947:Glucose