title: Cytokine modulation (IL-6, IL-8, IL-10) by human breast milk lipids on intestinal epithelial cells (Caco-2).
abstract: Human breast milk is the best form of nourishment for infants during the first year of life. It is composed by a complex mixture of carbohydrates, proteins and fats. Breast milk provides nutrients and bioactive factors that themselves modulate maturation and development of the gastrointestinal tract. Many studies have shown that it provides protection against gastrointestinal tract inflammation. In this sense, this study aimed to evaluate the effect of human breast milk lipids on epithelial intestinal cells (Caco-2) cytokine regulation and the fatty acid transporter protein (FATP) involved in this process. Caco-2 cells were cultivated and stimulated with different concentration of human milk lipids from healthy human mothers (18-30-year-olds) or single commercial lipids for 48 h. We measured the concentrations and mRNA levels of IL-6, IL-8 and IL-10 cytokines by immunoassay (ELISA) and quantitative-PCR (qRT-PCR) technique, respectively. We observed a two to three times decrease in pro-inflammatory cytokine levels (p < 0.01) as well as an increase in anti-inflammatory IL-10 levels in cells stimulated with increasing concentrations of breast milk lipids. These results suggest that human breast milk lipids could have an important role on the cytokine modulation in the newborn bowel.
authors.affiliation: a Facultad De Ciencias De La Salud , Universidad De Carabobo , Edo , Carabobo , República Bolivariana De Venezuela and.|b Laboratorio De Biotecnología Aplicada L.B.A , Edo , Carabobo , República Bolivariana De Venezuela.
authors.forename: Girolamo J
authors.initials: GJ
authors.lastname: Barrera
authors.affiliation: b Laboratorio De Biotecnología Aplicada L.B.A , Edo , Carabobo , República Bolivariana De Venezuela.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Sánchez
mesh_terms: D000293:Adolescent; D000328:Adult; D002097:C-Reactive Protein / Q000378:metabolism; D018938:Caco-2 Cells; D018929:Cell Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D016209:Interleukin-8 / Q000235:genetics*; D007413:Intestinal Mucosa / Q000187:drug effects; D008055:Lipids / Q000494:pharmacology; D008895:Milk, Human / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
chemical_list: C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D008055:Lipids; D012333:RNA, Messenger; D016753:Interleukin-10; D002097:C-Reactive Protein
keywords: Breast milk; cytokine; inflammation; lipids