title: Multiphase Oscillatory Flow Strategy for in Situ Measurement and Screening of Partition Coefficients.
abstract: Taking advantage of the difference between the surface energies of aqueous and organic solvents on a Teflon substrate, a fully automated small-scale strategy is developed on the basis of gas-driven oscillatory motion of a biphasic slug for high-throughput in situ measurement and screening of partition coefficients of organic substances between aqueous and organic phases. The developed oscillatory flow strategy enables single partition coefficient data point measurement within 8 min (including the sample preparation time) which is 360 times faster than the conventional "shake-flask" method, while using less than a 30 μL volume of the two phases and 9 nmol of the target organic substance. The developed multiphase strategy is validated using a conventional shake-flask technique. Finally, the developed strategy is extended to include automated screening of partition coefficients at physiological temperature.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, 66-342, Cambridge, Massachusetts 02139, United States.
authors.forename: Milad
authors.initials: M
authors.lastname: Abolhasani
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, 66-342, Cambridge, Massachusetts 02139, United States.
authors.forename: Connor W
authors.initials: CW
authors.lastname: Coley
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, 66-342, Cambridge, Massachusetts 02139, United States.
authors.forename: Klavs F
authors.initials: KF
authors.lastname: Jensen
mesh_terms: D001331:Automation; D057166:High-Throughput Screening Assays; D012997:Solvents / Q000737:chemistry*
chemical_list: D012997:Solvents