title: MgtE From Rhizobium leguminosarum Is a Mg²⁺ Channel Essential for Growth at Low pH and N2 Fixation on Specific Plants.
abstract: MgtE is predicted to be a Rhizobium leguminosarum channel and is essential for growth when both Mg²⁺ is limiting and the pH is low. N₂was only fixed at 8% of the rate of wild type when the crop legume Pisum sativum was inoculated with an mgtE mutant of R. leguminosarum and, although bacteroids were present, they were few in number and not fully developed. R. leguminosarum MgtE was also essential for N₂fixation on the native legume Vicia hirsuta but not when in symbiosis with Vicia faba. The importance of MgtE and the relevance of the contrasting phenotypes is discussed.
authors.affiliation: 1 Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, U.K.; and.
authors.forename: Graham
authors.initials: G
authors.lastname: Hood
authors.affiliation: 1 Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, U.K.; and.
authors.forename: Ramakrishnan
authors.initials: R
authors.lastname: Karunakaran
authors.affiliation: 1 Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, U.K.; and.
authors.forename: J Allan
authors.initials: JA
authors.lastname: Downie
authors.affiliation: 1 Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, U.K.; and.|2 Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, U.K.
authors.forename: Philip
authors.initials: P
authors.lastname: Poole
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism / Q000502:physiology*; D007473:Ion Channels / Q000378:metabolism / Q000502:physiology*; D008274:Magnesium / Q000378:metabolism* / Q000502:physiology; D009586:Nitrogen Fixation / Q000502:physiology*; D016961:Rhizobium leguminosarum / Q000502:physiology*; D031306:Vicia / Q000254:growth & development* / Q000382:microbiology / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D007473:Ion Channels; D008274:Magnesium