title: A glucuronoxylan-specific xylanase from a new Paenibacillus favisporus strain isolated from tropical soil of Brazil.
abstract: A new xylanolytic strain, Paenibacillus favisporus CC02-N2, was isolated from sugarcane plantation fields in Brazil. The strain had a xylan-degrading system with multiple enzymes, one of which, xylanase Xyn30A, was identified and characterized. The enzyme is a single-domain xylanase belonging to family 30 of the glycosyl hydrolases (GH30). Xyn30A shows high activity on glucuronoxylans, with a Vmax of 267.2 U mg⁻¹, a Km of 4.0 mg/ml, and a kcat of 13,333 min⁻¹ on beechwood xylan, but it does not hydrolyze arabinoxylans. The three-dimensional structure of Xyn30A consists of a common (β/α)8 barrel linked to a side-chain-associated β-structure, similar to previously characterized GH30 xylanases. The hydrolysis products from glucuronoxylan were methylglucuronic-acid-substituted xylooligomers (acidic xylooligosaccharides). The enzyme bound to insoluble xylan but not to crystalline cellulose. Our results suggest a specific role for Xyn30A in xylan biodegradation in natural habitats. The enzyme is a good candidate for the production of tailored xylooligosaccharides for use in the food industry and in the biotechnological transformation of biomass.
authors.affiliation: Department of Biotechnology, Biotechnology Center, Federal University of Paraíba, João Pessoa, Brazil.
authors.forename: Itácio Q M
authors.initials: IQ
authors.lastname: Padilha
authors.affiliation: Department of Microbiology, Faculty of Biology. University of Barcelona, Barcelona, Spain.
authors.forename: Susana V
authors.initials: SV
authors.lastname: Valenzuela
authors.affiliation: Department of Biotechnology, Biotechnology Center, Federal University of Paraíba, João Pessoa, Brazil.
authors.forename: Teresa C S L
authors.initials: TC
authors.lastname: Grisi
authors.affiliation: Department of Microbiology, Faculty of Biology. University of Barcelona, Barcelona, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Díaz
authors.affiliation: Department of Microbiology, Faculty of Biology. University of Barcelona, Barcelona, Spain.
authors.forename: Demetrius A M
authors.initials: DA
authors.lastname: de Araújo
authors.affiliation: Department of Microbiology, Faculty of Biology. University of Barcelona, Barcelona, Spain.
authors.forename: Francisco I Javier
authors.initials: FI
authors.lastname: Pastor
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001938:Brazil; D003001:Cloning, Molecular; D004795:Enzyme Stability; D007700:Kinetics; D008969:Molecular Sequence Data; D056507:Paenibacillus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D012988:Soil Microbiology; D013379:Substrate Specificity; D014995:Xylosidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D014995:Xylosidases
keywords: Paenibacillus favisporus; glycosyl hydrolases GH30; xylanase