title: Establishment from the snout and kidney of goldfish, Carassius auratus, of two new cell lines and their susceptibility to infectious pancreatic necrosis virus.
abstract: Goldfish Carassius auratus are commonly used in scientific research and have a significant economic value in the pet trade. In this study, two cell lines were established from the snout and kidney tissue of goldfish, in order to create a biological monitoring tool for viral diseases. Cell lines were optimally maintained at 25 °C in M199 medium supplemented with 15-20 % fetal bovine serum. Chromosomal analysis indicated that both cell lines remained diploid, with a mean chromosomal count of 100. Results of viral inoculation assays revealed that both cell lines shared similar patterns of viral susceptibility and production to infectious pancreatic necrosis virus (IPNV). The viral titers of IPNV in goldfish snout cell line (GFSe) and goldfish kidney cell line (GFKf) reached 10(6.8) and 10(5.9) TCID50/0.1 mL, respectively, within 7 days. The cytopathic effect could be observed when Cyprinid herpesvirus 2 was inoculated into these cells; however, the CPE disappeared after four passages. No CPE was observed in the cells infected by spring viremia carp virus or grass carp reovirus. These newly established cell lines will be a useful diagnostic tool for viral diseases in this fish species and also for the isolation and study of goldfish viruses in future.
authors.affiliation: Research Centre of Aquatic Animal Diseases, Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, 100029, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Jing
authors.affiliation: Shekou in Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, 518067, China.
authors.forename: Longying
authors.initials: L
authors.lastname: Gao
authors.affiliation: Research Centre of Aquatic Animal Diseases, Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, 100029, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Research Centre of Aquatic Animal Diseases, Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, 100029, China.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.affiliation: Research Centre of Aquatic Animal Diseases, Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, 100029, China.
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Lin
authors.affiliation: Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 100026, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Research Centre of Aquatic Animal Diseases, Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, 100029, China. sqwu@sina.com.
authors.forename: Shaoqiang
authors.initials: S
authors.lastname: Wu
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000662:veterinary; D002460:Cell Line / Q000821:virology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D002875:Chromosomes; D005393:Fish Diseases; D006054:Goldfish; D018061:Infectious pancreatic necrosis virus / Q000472:pathogenicity*; D007668:Kidney / Q000166:cytology*; D009666:Nose / Q000166:cytology*
keywords: Cell lines; Goldfish (Carassius auratus); Goldfish kidney cell line (GFKf); Goldfish snout cell line (GFSe)