title: Tumor-Infiltrating Immune Cells Are Associated With Prognosis of Gastric Cancer.
abstract: Immune cells contribute to determining the prognosis of gastric cancer. However, their exact role is less clear. We determined the prognostic significance of different immune cells in intratumoral tissue (T), stromal tissue (S), and adjacent normal tissue (N) of 166 gastric cancer cases and their interactions, including CD3+, CD4+, CD8, CD57+, CD68+, CD66b+, and Foxp3+ cells, and established an effective prognostic nomogram based on the immune reactions. We found high densities of TCD3+, TCD4+, TCD8+, SCD3+, SCD4+, SCD57+, SCD66b+, and NFoxp3+ cells, as well as high TCD8+/SCD8+ ratio, TCD68+/SCD68+ ratio, TCD3+/TFoxp3+ ratio, TCD4+/TFoxp3+ ratio, TCD8+/TFoxp3+ ratio, SCD3+/SFoxp3+ ratio, and SCD4+/SCD8+ ratio were associated with better survival, whereas high densities of TCD66b+, TFoxp3+, SFoxp3+ and NCD66b+ cells as well as high TCD57+/SCD57+ ratio, TCD66b+/SCD66b+ ratio, SCD8+/SFoxp3+ ratio, and TFoxp3+/NFoxp3+ ratio were associated with significantly worse outcome. Multivariate analysis indicated that tumor size, longitudinal tumor location, N stage, TCD68+/SCD68+ ratio, TCD8+/TFoxp3+ ratio, density of TFoxp3+ cells, and TCD66b+/SCD66b+ ratio were independent prognostic factors, which were all selected into the nomogram. The calibration curve for likelihood of survival demonstrated favorable consistency between predictive value of the nomogram and actual observation. The C-index (0.83, 95% CI: 0.78 to 0.87) of our nomogram for predicting prognosis was significantly higher than that of TNM staging system (0.70). Collectively, high TCD68+/SCD68+ ratio and TCD8+/TFoxp3+ ratio were associated with improved overall survival, whereas high density of TFoxp3+ cells and TCD66b+/SCD66b+ ratio demonstrated poor overall survival, which are promising independent predictors for overall survival in gastric cancer.
authors.affiliation: From the Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, China (KL,KY, BW,HC, XC, XC, LX, WZ, ZZ, JH); Laboratory of Gastric Cancer, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, China (KL,KY, BW,HC, XC, XC, LX, WZ, JH); Department of Pathology, West China Hospital, Sichuan University, China (LJ, DH); Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (FY); Department of Oncology, West China Hospital, Sichuan University, China (QL); and Laboratory of Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, China (XM).
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: HaiNing
authors.initials: H
authors.lastname: Chen
authors.forename: XiaoLong
authors.initials: X
authors.lastname: Chen
authors.forename: XinZu
authors.initials: X
authors.lastname: Chen
authors.forename: LiLi
authors.initials: L
authors.lastname: Jiang
authors.forename: FuXiang
authors.initials: F
authors.lastname: Ye
authors.forename: Du
authors.initials: D
authors.lastname: He
authors.forename: ZhengHao
authors.initials: Z
authors.lastname: Lu
authors.forename: Lian
authors.initials: L
authors.lastname: Xue
authors.forename: WeiHan
authors.initials: W
authors.lastname: Zhang
authors.forename: Qiu
authors.initials: Q
authors.lastname: Li
authors.forename: ZongGuang
authors.initials: Z
authors.lastname: Zhou
authors.forename: XianMing
authors.initials: X
authors.lastname: Mo
authors.forename: JianKun
authors.initials: J
authors.lastname: Hu
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology* / Q000401:mortality / Q000473:pathology*; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D018655:Lymphocyte Count; D016246:Lymphocytes, Tumor-Infiltrating / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000276:immunology* / Q000401:mortality / Q000473:pathology*; D055815:Young Adult
chemical_list: D015703:Antigens, CD; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors