title: Identification of a highly sulfated fucoidan from sea cucumber Pearsonothuria graeffei with well-repeated tetrasaccharides units.
abstract: Sea cucumber fucoidan is a major bioactive component of sea cucumber. The structures of fucoidans have significant influences on their biological activities. The present study clarified the delicate structure of a fucoidan from Pearsonothuria graeffei. Fucoidan was obtained after papain digestion and purified by ion chromatography. The carbohydrate sequence of fucoidan was firstly determined by negative-ion electrospray tandem mass spectrometry (ES-MS) with collision-induced dissociation of the oligosaccharide fragments, which were obtained by mild acid hydrolysis, and completed by NMR for assignment of the anomeric conformation. It was unambiguously identified as a tetrasaccharide repeating unit with a backbone of [ → 3Fuc (2S, 4S) α1 → 3Fucα1→ 3Fuc (4S) α1 → 3Fuc#7 × 10#]n. The glycosidic bonds between the non-sulfated and 2,4-O-disulfated fucose residues were selectively cleaved, and highly ordered oligosaccharide fragments with a tetrasaccharide repeating unit were obtained. The highly 4-O- and 2, 4-di-O-sulfated polysaccharide deserves further developments for Pharmacia use.
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Hu
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xingqian
authors.initials: X
authors.lastname: Ye
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Ding
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Donghong
authors.initials: D
authors.lastname: Liu
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jianchu
authors.initials: J
authors.lastname: Chen
authors.affiliation: Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Ge
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: chenshiguo210@163.com.
authors.forename: Shiguo
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012616:Sea Cucumbers / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides; D013431:Sulfates; C007789:fucoidan
keywords: ES–MS–MS; Fucoidan; NMR; Sea cucumber; Structure