title: Role of JAK-STAT signaling in maturation of phagosomes containing Staphylococcus aureus.
abstract: Phagocytosis is a required mechanism for the defense against pathogens. Staphylococcus aureus, an important bacterial pathogen, can promptly escape from phagosomes and proliferate within the cytoplasm of host. However, the mechanism of phagocytosis against S. aureus has not been intensively investigated. In this study, the S. aureus was engulfed by macrophages (RAW264.7 cells) but not digested by the cells, suggesting that the phagosomes did not maturate in macrophages. Further investigation revealed that peptidoglycan (PG) induced the phagosome maturation of macrophages, resulting in the eradication of S. aureus. Genome-wide analysis and quantitative real-time PCR indicated that the JAK-STAT pathway was activated by PG during the phagosome maturation of macrophages against S. aureus. This finding presented that the PG-activated JAK-STAT pathway was required for phagosome maturation. Therefore, our study contributed evidence that revealed a novel aspect of PG-triggered JAK-STAT pathway in the phagosome maturation of macrophages.
authors.affiliation: Collaborative Innovation Center of Deep Sea Biology, Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences, Zhejiang University, Hangzhou 310058, China.|College of Animal Science and Technology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Collaborative Innovation Center of Deep Sea Biology, Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Collaborative Innovation Center of Deep Sea Biology, Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Collaborative Innovation Center of Deep Sea Biology, Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D053613:Janus Kinase 1 / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D010587:Phagocytosis / Q000502:physiology; D010588:Phagosomes / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050794:STAT1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013203:Staphylococcal Infections / Q000235:genetics* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D050794:STAT1 Transcription Factor; D053613:Janus Kinase 1