title: Emergence of ST147 Klebsiella pneumoniae Producing OXA-204 Carbapenemase in a University Hospital, Tunisia.
abstract: Molecular features of the first carbapenem-resistant Klebsiella pneumoniae isolates (KP1 and KP2) from the University Hospital Tahar Sfar, Tunisia, were investigated. Antimicrobial susceptibility testing, multilocus sequence typing, S1 nuclease pulsed-field gel electrophoresis, Southern blot, and polymerase chain reaction (PCR)-based replicon typing were performed. Extended-spectrum β-lactamases and carbapenemase genes were detected by PCR and sequencing. Both isolates were multidrug resistant. KP1 was of sequence type (ST) ST101 and exhibited blaCTX-M-15 and blaTEM-1 on an untypeable plasmid and blaOXA-48 on an IncL/M plasmid. KP2 was genetically unrelated to KP1 (ST147) and harbored an IncA/C plasmid carrying blaCMY-4 and the blaOXA-48 derivative gene: blaOXA-204. This study reports the second case worldwide of an OXA-204-producing K. pneumoniae isolate from the same country, however, in a different genetic background.
authors.affiliation: 1 Unité Antibiorésistance et Virulence Bactériennes , ANSES Site de Lyon, Lyon, France .|2 Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives , Faculté de Pharmacie, Monastir, Tunisie.
authors.forename: Raoudha
authors.initials: R
authors.lastname: Grami
authors.affiliation: 2 Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives , Faculté de Pharmacie, Monastir, Tunisie.|3 Unité de Recherche: Résistances Bactériennes Émergentes et Sécurité des Soins "UR12SP37," Laboratoire de Microbiologie, Hôpital Universitaire Sahloul , Sousse, Tunisie.
authors.forename: Wejdene
authors.initials: W
authors.lastname: Mansour
authors.affiliation: 4 Laboratoire de Microbiologie, Hopital Tahar Sfar , Mahdia, Tunisie.
authors.forename: Anis
authors.initials: A
authors.lastname: Ben Haj Khalifa
authors.affiliation: 3 Unité de Recherche: Résistances Bactériennes Émergentes et Sécurité des Soins "UR12SP37," Laboratoire de Microbiologie, Hôpital Universitaire Sahloul , Sousse, Tunisie.
authors.forename: Safia
authors.initials: S
authors.lastname: Dahmen
authors.affiliation: 1 Unité Antibiorésistance et Virulence Bactériennes , ANSES Site de Lyon, Lyon, France .
authors.forename: Pierre
authors.initials: P
authors.lastname: Chatre
authors.affiliation: 1 Unité Antibiorésistance et Virulence Bactériennes , ANSES Site de Lyon, Lyon, France .
authors.forename: Marisa
authors.initials: M
authors.lastname: Haenni
authors.affiliation: 2 Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives , Faculté de Pharmacie, Monastir, Tunisie.
authors.forename: Mahjoub
authors.initials: M
authors.lastname: Aouni
authors.affiliation: 1 Unité Antibiorésistance et Virulence Bactériennes , ANSES Site de Lyon, Lyon, France .
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Madec
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D015870:Gene Expression; D006785:Hospitals, University; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007710:Klebsiella Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D014416:Tunisia / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D004269:DNA, Bacterial; D007527:Isoenzymes; D001618:beta-Lactamases