title: Sodium tanshinone IIA sulfonate affects Marek's disease virus replication by inhibiting gB expression.
abstract: BACKGROUND
  Previous studies demonstrated that sodium tanshinone IIA sulfonate (STS) could inhibit MDV replication in vitro. The mechanism about how STS inhibits MDV replication is still not well understood.
  
  
  OBJECTIVE
  In this study, we evaluated the effect of STS on gB gene/protein of Marek's disease virus (MDV).
  
  
  METHODS
  The concentration of 0.25 mg/ml of STS was used in this study. Meanwhile, 0.25 mg/ml of acyclovir (ACV) was used as a positive control. About 9-11-d-old embryonated specific-pathogen-free (SPF) chicken eggs were used to prepare CEF cells. CEF cells were infected with MDV 2 h, followed by treatment with STS. Real-time PCR and western blot assay were used to measure the gB (UL27) gene/protein expression in STS treatment group at 24, 48, 72, and 96 h post-infection.
  
  
  RESULTS
  Compared with MDV control, the gB gene copies were significantly decreased in STS and ACV treatment groups at 72 h and 96 h (p < 0.05), both in the DNA and in the mRNA level. Furthermore, the expression of gB protein was also inhibited by STS at 24, 72, and 96 h.
  
  
  CONCLUSIONS
  Our study demonstrated that STS could effectively inhibit the MDV replication by suppressing gB gene/protein expression in cell culture.
authors.affiliation: a College of Animal Science and Veterinary Medicine, Shanxi Agricultural University , Shanxi , PR China .
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: a College of Animal Science and Veterinary Medicine, Shanxi Agricultural University , Shanxi , PR China .
authors.forename: Panpan
authors.initials: P
authors.lastname: Sun
authors.affiliation: a College of Animal Science and Veterinary Medicine, Shanxi Agricultural University , Shanxi , PR China .
authors.forename: Yuning
authors.initials: Y
authors.lastname: Feng
authors.affiliation: b Department of Pathobiology , College of Veterinary Medicine, Texas A&M University, College Station , TX , USA .
authors.forename: Jianhua
authors.initials: J
authors.lastname: Guo
authors.affiliation: a College of Animal Science and Veterinary Medicine, Shanxi Agricultural University , Shanxi , PR China .
authors.forename: Yaogui
authors.initials: Y
authors.lastname: Sun
authors.affiliation: c School of Chinese Pharmacy, Beijing University of Chinese Medicine , Beijing , PR China , and.
authors.forename: Haimin
authors.initials: H
authors.lastname: Lei
authors.affiliation: d College of Veterinary Medicine, China Agricultural University (CAU) , Beijing , PR China.
authors.forename: Jianqin
authors.initials: J
authors.lastname: Xu
authors.affiliation: a College of Animal Science and Veterinary Medicine, Shanxi Agricultural University , Shanxi , PR China .
authors.forename: Hongquan
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D002642:Chick Embryo; D015967:Gene Expression Regulation, Viral / Q000187:drug effects* / Q000502:physiology; D008380:Marek Disease / Q000235:genetics / Q000378:metabolism*; D010616:Phenanthrenes / Q000494:pharmacology*; D014759:Viral Envelope Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects* / Q000502:physiology
chemical_list: D000956:Antigens, Viral; D010616:Phenanthrenes; D014759:Viral Envelope Proteins; C072811:glycoprotein B, Marek's disease virus; C024894:tanshinone II A sodium sulfonate
keywords: Anti-MDV; CEF cells; traditional Chinese medicine