title: Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences.
abstract: OBJECTIVE
  Chloroplast microsatellite loci were characterized from transcriptomes of Artocarpus altilis (breadfruit) and A. camansi (breadnut). They were tested in A. odoratissimus (terap) and A. altilis and evaluated in silico for two congeners.
  
  
  RESULTS
  Fifteen simple sequence repeats (SSRs) were identified in chloroplast sequences from four Artocarpus transcriptome assemblies. The markers were evaluated using capillary electrophoresis in A. odoratissimus (105 accessions) and A. altilis (73). They were also evaluated in silico in A. altilis (10), A. camansi (6), and A. altilis × A. mariannensis (7) transcriptomes. All loci were polymorphic in at least one species, with all 15 polymorphic in A. camansi. Per species, average alleles per locus ranged between 2.2 and 2.5. Three loci had evidence of fragment-length homoplasy.
  
  
  CONCLUSIONS
  These markers will complement existing nuclear markers by enabling confident identification of maternal and clone lines, which are often important in vegetatively propagated crops such as breadfruit.
authors.affiliation: Department of Plant Science, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA ; Plant Biology and Conservation, Northwestern University, 2205 Tech Drive, Hogan 2-144, Evanston, Illinois 60208 USA.
authors.forename: Elliot M
authors.initials: EM
authors.lastname: Gardner
authors.affiliation: Department of Plant Science, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA ; Plant Biology and Conservation, Northwestern University, 2205 Tech Drive, Hogan 2-144, Evanston, Illinois 60208 USA.
authors.forename: Kristen M
authors.initials: KM
authors.lastname: Laricchia
authors.affiliation: Department of Biology, Illinois College, 1101 West College Avenue, Jacksonville, Illinois 62650 USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Murphy
authors.affiliation: Breadfruit Institute, National Tropical Botanical Garden, Kahaleo, Hawai'i 96741 USA.
authors.forename: Diane
authors.initials: D
authors.lastname: Ragone
authors.affiliation: USDA, ARS, Genomics and Bioinformatics Research Unit, 141 Experiment Station Road, Stoneville, Mississippi 38776-0350 USA.
authors.forename: Brian E
authors.initials: BE
authors.lastname: Scheffler
authors.affiliation: USDA, ARS, Genomics and Bioinformatics Research Unit, 141 Experiment Station Road, Stoneville, Mississippi 38776-0350 USA.
authors.forename: Sheron
authors.initials: S
authors.lastname: Simpson
authors.affiliation: Department of Plant Science, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA.
authors.forename: Evelyn W
authors.initials: EW
authors.lastname: Williams
authors.affiliation: Department of Plant Science, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA ; Plant Biology and Conservation, Northwestern University, 2205 Tech Drive, Hogan 2-144, Evanston, Illinois 60208 USA.
authors.forename: Nyree J C
authors.initials: NJ
authors.lastname: Zerega
keywords: Artocarpus altilis; Artocarpus camansi; Artocarpus mariannensis; Artocarpus odoratissimus; Moraceae; breadfruit