title: Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering.
abstract: We developed tricalcium phosphate-chitosan-fucoidan biocomposite scaffold (TCP-Ch-Fu) by using the freeze-drying technique. The fabricated biocomposite scaffolds were analyzed by spectroscopy and porosity measurement. The biomechanical properties of scaffolds were assessed by compression test and the results suggested that the incorporation of Fucoidan into the biocomposite improves the compression strength of scaffolds. Biomineralization of scaffolds was evaluated by soaking them in simulated body fluid and the results revealed that the addition of Fucoidan into the scaffolds enhanced the formation of apatite layer on the surface of biocomposite after 7 days of immersion. Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold. The addition of Fucoidan to TCP-Ch increased the release of osteocalcin, confirming that it can support osteogenic differentiation of human mesenchymal stromal cells in in vitro culture. Thus, TCP-Ch-Fu could be a potential candidate for bone-tissue engineering applications.
authors.affiliation: Tissue Engineering Group (TEG), Department of Orthopaedic Surgery, NOCERAL, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Subramaniam
authors.initials: S
authors.lastname: Puvaneswary
authors.affiliation: Department of Mechanical engineering, Engineering Faculty, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Sepehr
authors.initials: S
authors.lastname: Talebian
authors.affiliation: Tissue Engineering Group (TEG), Department of Orthopaedic Surgery, NOCERAL, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: hbr_bala@yahoo.com.
authors.forename: Hanumantharao Balaji
authors.initials: HB
authors.lastname: Raghavendran
authors.affiliation: Tissue Engineering Group (TEG), Department of Orthopaedic Surgery, NOCERAL, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Malliga Raman
authors.initials: MR
authors.lastname: Murali
authors.affiliation: Department of Mechanical engineering, Engineering Faculty, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Mehrali
authors.affiliation: Department of Mechanical engineering, Engineering Faculty, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Amalina Muhammad
authors.initials: AM
authors.lastname: Afifi
authors.affiliation: Department of Restorative Dentistry, Faculty of Dentistry Building, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Noor Hayaty Binti Abu
authors.initials: NH
authors.lastname: Kasim
authors.affiliation: Tissue Engineering Group (TEG), Department of Orthopaedic Surgery, NOCERAL, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Tunku
authors.initials: T
authors.lastname: Kamarul
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology* / Q000187:drug effects; D002130:Calcium Phosphates / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D005612:Freeze Drying; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D055595:Mechanical Phenomena; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D008903:Minerals / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D002130:Calcium Phosphates; D008903:Minerals; D011134:Polysaccharides; D048271:Chitosan; C007789:fucoidan; C018392:tricalcium phosphate
keywords: Biocomposite; Bone tissue engineering; Chitosan; Freeze-drying; Fucoidan