title: Whole Genomic Analysis of an Unusual Human G6P[14] Rotavirus Strain Isolated from a Child with Diarrhea in Thailand: Evidence for Bovine-To-Human Interspecies Transmission and Reassortment Events.
abstract: An unusual rotavirus strain, SKT-27, with the G6P[14] genotypes (RVA/Human-wt/THA/SKT-27/2012/G6P[14]), was identified in a stool specimen from a hospitalized child aged eight months with severe diarrhea. In this study, we sequenced and characterized the complete genome of strain SKT-27. On whole genomic analysis, strain SKT-27 was found to have a unique genotype constellation: G6-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. The non-G/P genotype constellation of this strain (I2-R2-C2-M2-A3-N2-T6-E2-H3) is commonly shared with rotavirus strains from artiodactyls such as cattle. Phylogenetic analysis indicated that nine of the 11 genes of strain SKT-27 (VP7, VP4, VP6, VP2-3, NSP1, NSP3-5) appeared to be of artiodactyl (likely bovine) origin, while the remaining VP1 and NSP2 genes were assumed to be of human origin. Thus, strain SKT-27 was found to have a bovine rotavirus genetic backbone, and thus is likely to be of bovine origin. Furthermore, strain SKT-27 appeared to be derived through interspecies transmission and reassortment events involving bovine and human rotavirus strains. Of note is that the VP7 gene of strain SKT-27 was located in G6 lineage-5 together with those of bovine rotavirus strains, away from the clusters comprising other G6P[14] strains in G6 lineages-2/6, suggesting the occurrence of independent bovine-to-human interspecies transmission events. To our knowledge, this is the first report on full genome-based characterization of human G6P[14] strains that have emerged in Southeast Asia. Our observations will provide important insights into the origin of G6P[14] strains, and into dynamic interactions between human and bovine rotavirus strains.
authors.affiliation: Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
authors.forename: Ratana
authors.initials: R
authors.lastname: Tacharoenmuang
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Komoto
authors.affiliation: Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
authors.forename: Ratigorn
authors.initials: R
authors.lastname: Guntapong
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Tomihiko
authors.initials: T
authors.lastname: Ide
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Haga
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Katayama
authors.affiliation: Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Takema
authors.initials: T
authors.lastname: Kato
authors.affiliation: Genome and Transcriptome Analysis Center, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Ouchi
authors.affiliation: Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan; Genome and Transcriptome Analysis Center, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kurahashi
authors.affiliation: Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Tsuji
authors.affiliation: Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
authors.forename: Somchai
authors.initials: S
authors.lastname: Sangkitporn
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D002417:Cattle; D003967:Diarrhea / Q000821:virology*; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D007223:Infant; D010802:Phylogeny; D016865:Reassortant Viruses / Q000145:classification* / Q000235:genetics / Q000502:physiology; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000502:physiology; D012400:Rotavirus Infections / Q000235:genetics* / Q000635:transmission* / Q000821:virology; D013785:Thailand