title: In vitro evaluation of γδ T cells regulatory function in Behçet's disease patients and healthy controls.
abstract: CD8-positive γδ T lymphocytes (GDCD8(+)) are specifically increased in peripheral blood of Behçet's disease (BD) patients. GDCD8(+) have shown a T regulatory (Treg) function in autoimmune experimental models, human tumor infiltrates and intestinal intraepithelial lymphocytes from celiac patients. The aim of this study was to evaluate the Treg function of GDCD8(+) and GDCD8(-), freshly isolated from peripheral blood, in comparison to CD4(+)CD25(high) naturally occurring Treg cells (nTreg) in BD and healthy controls (HC). We tested their suppressive activity on CD4(+)CD25(-) T effector cells (Teff) proliferation by a CFSE dilution protocol, after suboptimal activation with anti-CD3, in the absence or presence of IL-2. Furthermore, secreted cytokines and suppressive latency associated peptide (LAP)-TGFβ surface upregulation were determined after GD activation. We found that Vδ1 chains contribution to GDCD8(+) was higher in BD than in HC, but neither GDCD8(+) nor GDCD8(-); (i) suppressed Teff proliferation, (ii) expressed LAP-TGFβ (iii) nor secreted IL-10, in either group. Moreover, GD presented a proinflammatory cytokine profile, mainly producing IFNγ and TNFα, in contrast to nTregs. In conclusion, peripheral GD could contribute more to the dysregulation of TH1 type of cytokines than to exerting a Treg function in BD.
authors.affiliation: Fundació d'Investigació Sanitària de les Illes Balears, Hospital Universitari Son Espases, Spain; Institut d'Investigació Sanitària de Palma (IdISPa), Hospital Universitari Son Espases, Spain. Electronic address: ACLEMENTE@clinic.ub.es.
authors.forename: Antonio
authors.initials: A
authors.lastname: Clemente Ximenis
authors.affiliation: Institut d'Investigació Sanitària de Palma (IdISPa), Hospital Universitari Son Espases, Spain; Unitat d'Investigació, Hospital Universitari Son Espases, Spain. Electronic address: catalina.crespib@ssib.es.
authors.forename: Catalina
authors.initials: C
authors.lastname: Crespí Bestard
authors.affiliation: Immunology Department, Hospital Universitari Son Espases, Spain. Electronic address: ainacambra@hotmail.com.
authors.forename: Ana
authors.initials: A
authors.lastname: Cambra Conejero
authors.affiliation: Institut d'Investigació Sanitària de Palma (IdISPa), Hospital Universitari Son Espases, Spain; Autoimmune Diseases Unit of the Internal Medicine Department, Hospital Universitari Son Espases, Spain. Electronic address: lucio.pallares@ssib.es.
authors.forename: Lucio
authors.initials: L
authors.lastname: Pallarés Ferreres
authors.affiliation: Rheumatology Department, Hospital Son Llàtzer, Spain. Electronic address: ajuan@hsll.es.
authors.forename: Antonio
authors.initials: A
authors.lastname: Juan Mas
authors.affiliation: Institut d'Investigació Sanitària de Palma (IdISPa), Hospital Universitari Son Espases, Spain; Ophthalmology Department, Hospital Universitari Son Espases, Spain. Electronic address: josel.olea@ssib.es.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Olea Vallejo
authors.affiliation: Institut d'Investigació Sanitària de Palma (IdISPa), Hospital Universitari Son Espases, Spain; Immunology Department, Hospital Universitari Son Espases, Spain. Electronic address: rosa.julia@ssib.es.
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Julià Benique
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology*; D001528:Behcet Syndrome / Q000276:immunology*; D005260:Female; D006801:Humans; D007165:Immunosuppression Therapy; D018836:Inflammation Mediators / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D058408:Th1-Th2 Balance; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D018836:Inflammation Mediators; D016692:Receptors, Antigen, T-Cell, gamma-delta; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma
keywords: Behçet’s disease; CD8; Gamma delta T lymphocytes; T regulatory function