title: Biophysical properties of carboxymethyl derivatives of mannan and dextran.
abstract: Mannan from Candida albicans, dextran from Leuconostoc spp. and their carboxymethyl (CM)-derivatives were tested on antioxidant and thrombolytic activities. As antioxidant tests, protection of liposomes against OH radicals and reducing power assay were used. Dextran and mannan protected liposomes in dose-dependent manner. Carboxymethylation significantly increased antioxidant properties of both CM-derivatives up to concentration of 10mg/mL, higher concentrations did not change the protection of liposomes. The reducing power of CM-mannan (DS 0.92) was significantly lower (P<0.05) than underivatized mannan. No reductive activity was found for dextran and CM-dextran. All CM-derivatives demonstrated statistically significant increasing activity compared with underivatized polysaccharides. The highest thrombolytic activity was found using CM-mannan (DS 0.92). The clot lysis here amounted to 68.78 ± 6.52% compared with 0.9% NaCl control (18.3 ± 6.3%). Three-dimensional surface profiles of mannan, dextran, and their CM-derivatives were compared by atomic force microscopy.
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Immunochemistry of Glycoconjugates, Dúbravská cesta 9, 84538 Bratislava, Slovak Republic. Electronic address: jana.korcova@savba.sk.
authors.forename: Jana
authors.initials: J
authors.lastname: Korcová
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Immunochemistry of Glycoconjugates, Dúbravská cesta 9, 84538 Bratislava, Slovak Republic. Electronic address: eva.machova@savba.sk.
authors.forename: Eva
authors.initials: E
authors.lastname: Machová
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Immunochemistry of Glycoconjugates, Dúbravská cesta 9, 84538 Bratislava, Slovak Republic. Electronic address: jaroslav.filip@savba.sk.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Filip
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Immunochemistry of Glycoconjugates, Dúbravská cesta 9, 84538 Bratislava, Slovak Republic. Electronic address: slavomir.bystricky@savba.sk.
authors.forename: Slavomír
authors.initials: S
authors.lastname: Bystrický
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D002176:Candida albicans / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry; D007934:Leuconostoc / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D008297:Male; D008351:Mannans / Q000737:chemistry / Q000494:pharmacology*; D018625:Microscopy, Atomic Force; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D003911:Dextrans; D005343:Fibrinolytic Agents; D008081:Liposomes; D008351:Mannans; D017665:Hydroxyl Radical; C014392:carboxymethyl dextran
keywords: Antioxidant; Atomic force microscopy; C. albicans; Dextran; Mannan; Thrombolysis