title: Imaging Immunosenescence.
abstract: To demonstrate effects of aging visually requires a robust technique that can reproducibly detect small differences in efficiency or kinetics between groups. Investigators of aging will greatly appreciate the benefits of Amnis ImageStream technology ( www.amnis.com/), which combines quantitative flow cytometry with simultaneous high-resolution digital imaging. Imagestream is quantitative, reproducible, feasible with limited samples, and it facilitates in-depth examination of cellular mechanisms between cohorts of samples.
authors.affiliation: Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, 300 Cedar Street/TAC S413, New Haven, CT, 06520, USA.|State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, 200433, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qian
authors.affiliation: Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, 300 Cedar Street/TAC S413, New Haven, CT, 06520, USA. ruth.montgomery@yale.edu.
authors.forename: Ruth R
authors.initials: RR
authors.lastname: Montgomery
mesh_terms: D015415:Biomarkers; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique; D006801:Humans; D000068884:Immunosenescence / Q000502:physiology*; D008853:Microscopy / Q000379:methods*
chemical_list: D015415:Biomarkers
keywords: Aging; Dendritic cell; Flow cytometry; Image analysis; Immune response; Microscopy; Monocyte; Neutrophil; Signal transduction