title: Characterization of Antibodies to Identify Cellular Expression of Dopamine Receptor 4.
abstract: The dopamine receptor D4 (DRD4) plays an important role in vision. In order to study the DRD4 expression in vivo, it is important to have antibodies that are specific for DRD4 for both immunoblot and immunohistochemical (IHC) applications. In this study, six antibodies raised against DRD4 peptides were tested in vitro, using transfected mammalian cells, and in vivo, using mouse retinas. Three Santa Cruz (SC) antibodies, D-16, N-20, and R-20, were successful in IHC of transfected DRD4; however, N-20 was the only one effective on immunoblot analysis in DRD4 transfected cells and IHC of mouse retinal sections, while R-20, 2B9, and Antibody Verify AAS63631C were non-specific or below detection.
authors.affiliation: Departments of Ophthalmology, USC Eye Institute, Keck School of Medicine of the University of Southern California, Institute for Genetic Medicine, 2250 Alcazar St CSC 215, 90033, Los Angeles, CA, USA. jddeming@usc.edu.
authors.forename: Janise D
authors.initials: JD
authors.lastname: Deming
authors.affiliation: University of Ghent, Proeftuinstraat 86, 9000, Gent, Belgium. kathleen.vancraenenbroeck@ugent.be.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Van Craenenbroeck
authors.affiliation: Biomedical Engineering, Viterbi School of Engineering, University of Southern California, 1042 Downey way, DRB 140, 90007, Los Angeles, CA, USA. yuneom@usc.edu.
authors.forename: Yun Sung
authors.initials: YS
authors.lastname: Eom
authors.affiliation: Biomedical Engineering, Viterbi School of Engineering, University of Southern California, 1042 Downey way, DRB 140, 90007, Los Angeles, CA, USA. eunjinl@usc.edu.
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Departments of Ophthalmology and Cell & Neurobiology, USC Eye Institute, Keck School of Medicine of the University of Southern California, Institute for Genetic Medicine, 2250 Alcazar Street, CSC 135H, 90089-9075, Los Angeles, CA, USA. eyesightresearch@hotmail.com.
authors.forename: Cheryl Mae
authors.initials: CM
authors.lastname: Craft
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000378:metabolism; D000918:Antibody Specificity / Q000276:immunology*; D057809:HEK293 Cells; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D050638:Receptors, Dopamine D4 / Q000276:immunology* / Q000378:metabolism; D012160:Retina / Q000276:immunology* / Q000378:metabolism
chemical_list: D000906:Antibodies; C493599:DRD4 protein, human; D050638:Receptors, Dopamine D4
keywords: Antibody; Dopamine; Dopamine receptor D4; HEK; Immunoblot; Immunohistochemistry; Retina; Transfection