title: Relation of interleukin-10 Promoter Polymorphisms to Adult Chronic Immune Thrombocytopenic Purpura in a Cohort of Egyptian Population.
abstract: BACKGROUND
  Adult chronic immune thrombocytopenic purpura (chronic ITP) is an autoimmune multifactorial bleeding disorder that occurs because of enhanced peripheral platelet destruction. Treatment decisions can be challenging because the goal of treatment is to prevent severe bleeding, but the risk of bleeding can be difficult to estimate for any individual patient.
  
  
  OBJECTIVE
  This case-control study was planned to investigate the relationship of interleukin (IL)-10 promoter (IL-10-1082, -819 and -592) polymorphisms with the susceptibility, severity and outcome of adult chronic ITP in a cohort of Egyptian population.
  
  
  METHODS
  Typing of IL-10 promoter polymorphisms was done using restriction fragment length polymorphism for 62 adult patients with chronic ITP and 73 age- and sex-matched healthy controls.
  
  
  RESULTS
  No significant differences were found between ITP patients and controls regarding the frequency of IL-10 promoter genotypes, alleles or haplotypes. IL-10-592 AA genotype and ATA (IL-10-1082, -819 and -592) haplotype were associated with severe ITP (p = 0.003, 0.043, respectively).
  
  
  CONCLUSIONS
  Our findings suggest that the IL-10 promoter polymorphisms are unlikely to affect the development or treatment outcome of chronic adult ITP in Egyptian population, but IL-10-592 AA genotype and IL-10 (-1082, -819 and -592) ATA haplotype may be associated with disease severity. Because ITP is a complex disease, it is recommended that a multicenter study should be done with large sample size and unified typing technique.
authors.affiliation: a Department of Clinical Pathology, Faculty of Medicine , Mansoura University , Mansoura , Egypt .
authors.forename: Doaa
authors.initials: D
authors.lastname: El Ghannam
authors.affiliation: b Laboratory Medicine Department , Mansoura Fever Hospital , Mansoura , Egypt .
authors.forename: Iman M
authors.initials: IM
authors.lastname: Fawzy
authors.affiliation: c Department of Clinical Hematology , and.
authors.forename: Emad
authors.initials: E
authors.lastname: Azmy
authors.affiliation: d Internal Medicine Department, Faculty of Medicine, Mansoura University , Mansoura , Egypt.
authors.forename: Hazem
authors.initials: H
authors.lastname: Hakim
authors.affiliation: d Internal Medicine Department, Faculty of Medicine, Mansoura University , Mansoura , Egypt.
authors.forename: Islam
authors.initials: I
authors.lastname: Eid
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D002908:Chronic Disease; D004534:Egypt / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics* / Q000628:therapy
chemical_list: D016753:Interleukin-10
keywords: Egypt; immune thrombocytopenic purpura; interleukin 10; platelet; polymorphism