title: Tamoxifen-Containing Eye Drops Successfully Trigger Cre-Mediated Recombination in the Entire Eye.
abstract: Embryonic lethality in mice with targeted gene deletion is a major issue that can be circumvented by using Cre-loxP-based animal models. Various inducible Cre systems are available, e.g. such that are activated following tamoxifen treatment, and allow deletion of a specific target gene at any desired time point during the life span of the animal. In this study, we describe the efficiency of topical tamoxifen administration by eye drops using a Cre- reporter mouse strain (R26R). We report that tamoxifen-responsive CAGGCre-ER (TM) mice show a robust Cre- mediated recombination throughout the entire eye.
authors.affiliation: Institute of Human Anatomy and Embryology, University of Regensburg, Universitätsstr. 31, D-93053, Regensburg, Germany. Anja.Schlecht@vkl.uni-regensburg.de.
authors.forename: Anja
authors.initials: A
authors.lastname: Schlecht
authors.affiliation: Institute of Human Anatomy and Embryology, University of Regensburg, Universitätsstr. 31, D-93053, Regensburg, Germany. Sarah.Leimbeck@gmx.de.
authors.forename: Sarah V
authors.initials: SV
authors.lastname: Leimbeck
authors.affiliation: Institute of Human Anatomy and Embryology, University of Regensburg, Universitätsstr. 31, D-93053, Regensburg, Germany. Ernst.Tamm@vkl.uni-regensburg.de.
authors.forename: Ernst R
authors.initials: ER
authors.lastname: Tamm
authors.affiliation: Institute of Human Anatomy and Embryology, University of Regensburg, Universitätsstr. 31, D-93053, Regensburg, Germany. Barbara.Braunger@vkl.uni-regensburg.de.
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Braunger
mesh_terms: D000818:Animals; D004965:Estrogen Antagonists / Q000494:pharmacology; D005123:Eye / Q000187:drug effects* / Q000378:metabolism; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009883:Ophthalmic Solutions / Q000494:pharmacology; D011995:Recombination, Genetic / Q000187:drug effects*; D013629:Tamoxifen / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D004965:Estrogen Antagonists; D009883:Ophthalmic Solutions; D013629:Tamoxifen; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase
keywords: Cre; Cre-loxP; Eye; Eye drops; Retina; Tamoxifen