title: Use of carbonate extraction in analyzing moderately hydrophobic transmembrane proteins in the mitochondrial inner membrane.
abstract: Resistance to sodium carbonate extraction is regarded as a canonical way to distinguish integral membrane proteins (MPs) from other membrane-associated proteins. However, it has been observed that carbonate extraction releases some mitochondrial integral MPs. Here, by analyzing both artificially designed and native mitochondrial inner MPs containing transmembrane domains (TMDs) of different hydrophobicities, we show that carbonate treatment can release moderately hydrophobic TMDs from the mitochondrial inner membrane. These results suggest that resistance and sensitivity to carbonate extraction may be interpreted with caution when analyzing the nature of mitochondrial inner MPs.
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul, 08826, South Korea.
authors.forename: Hayoung
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul, 08826, South Korea.
authors.forename: Salomé Calado
authors.initials: SC
authors.lastname: Botelho
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul, 08826, South Korea.
authors.forename: Kwangjin
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul, 08826, South Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002254:Carbonates / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification*; D051336:Mitochondrial Membranes / Q000378:metabolism*
chemical_list: D002254:Carbonates; D008565:Membrane Proteins; C005686:sodium carbonate
keywords: carbonate extraction; integral membrane; membrane protein; mitochondria