title: Cucurbitacins from the Leaves of Citrullus colocynthis (L.) Schrad.
abstract: Two new tetracyclic cucurbitane-type triterpene glycosides were isolated from an ethyl acetate extract of Citrullus colocynthis leaves together with four known cucurbitacins. Their structures were established on the basis of their spectroscopic data (mainly NMR and mass spectrometry). Evaluation of the in vitro cytotoxic activity of the isolated compounds against two human colon cancer cell lines (HT29 and Caco-2) and one normal rat intestine epithelial cell line (IEC6), revealed that one of the isolated compounds presented interesting specific cytotoxic activity towards colorectal cell lines.
authors.affiliation: Université de Toulouse, UPS, Pharma-DEV, UMR 152, Université Toulouse 3, Faculté des Sciences Pharmaceutiques, F-31062 Toulouse cedex 09, France. chawech.rachid@gmail.com.|Institut de Recherche pour le Développement (IRD), UMR 152 Pharma-DEV, F-31062 Toulouse cedex 09, France. chawech.rachid@gmail.com.|Laboratoire de Chimie des Substances Naturelles, Faculté des Sciences de Sfax, Université de Sfax Route de l'aéroport, BP 1171, 3000 Sfax, Tunisie. chawech.rachid@gmail.com.
authors.forename: Rachid
authors.initials: R
authors.lastname: Chawech
authors.affiliation: Laboratoire de Chimie des Substances Naturelles, Faculté des Sciences de Sfax, Université de Sfax Route de l'aéroport, BP 1171, 3000 Sfax, Tunisie. raoudhajarraya@yahoo.fr.
authors.forename: Raoudha
authors.initials: R
authors.lastname: Jarraya
authors.affiliation: Université de Toulouse, UPS, Pharma-DEV, UMR 152, Université Toulouse 3, Faculté des Sciences Pharmaceutiques, F-31062 Toulouse cedex 09, France. cynthia.girardi@gmail.com.|Institut de Recherche pour le Développement (IRD), UMR 152 Pharma-DEV, F-31062 Toulouse cedex 09, France. cynthia.girardi@gmail.com.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Girardi
authors.affiliation: Université de Toulouse, UPS, Pharma-DEV, UMR 152, Université Toulouse 3, Faculté des Sciences Pharmaceutiques, F-31062 Toulouse cedex 09, France. marieke.vansteelandt@univ-tlse3.fr.|Institut de Recherche pour le Développement (IRD), UMR 152 Pharma-DEV, F-31062 Toulouse cedex 09, France. marieke.vansteelandt@univ-tlse3.fr.
authors.forename: Marieke
authors.initials: M
authors.lastname: Vansteelandt
authors.affiliation: Université de Toulouse, UPS, Pharma-DEV, UMR 152, Université Toulouse 3, Faculté des Sciences Pharmaceutiques, F-31062 Toulouse cedex 09, France. guillaume.marti@univ-tlse3.fr.|Institut de Recherche pour le Développement (IRD), UMR 152 Pharma-DEV, F-31062 Toulouse cedex 09, France. guillaume.marti@univ-tlse3.fr.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Marti
authors.affiliation: Laboratoire de Chimie des Substances Naturelles, Faculté des Sciences de Sfax, Université de Sfax Route de l'aéroport, BP 1171, 3000 Sfax, Tunisie. claire.racaud@inserm.fr.|INSERM U1043, CNRS U5282, Université de Toulouse, UPS, Centre de Physiopathologie de Toulouse Purpan, F-31300 Toulouse, France. claire.racaud@inserm.fr.
authors.forename: Imen
authors.initials: I
authors.lastname: Nasri
authors.affiliation: INSERM U1043, CNRS U5282, Université de Toulouse, UPS, Centre de Physiopathologie de Toulouse Purpan, F-31300 Toulouse, France. claire.racaud@inserm.fr.
authors.forename: Claire
authors.initials: C
authors.lastname: Racaud-Sultan
authors.affiliation: Université de Toulouse, UPS, Pharma-DEV, UMR 152, Université Toulouse 3, Faculté des Sciences Pharmaceutiques, F-31062 Toulouse cedex 09, France. nicolas.fabre@univ-tlse3.fr.|Institut de Recherche pour le Développement (IRD), UMR 152 Pharma-DEV, F-31062 Toulouse cedex 09, France. nicolas.fabre@univ-tlse3.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Fabre
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D065986:Citrullus colocynthis / Q000737:chemistry*; D054728:Cucurbitacins / Q000737:chemistry* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D054728:Cucurbitacins
keywords: Citrullus colocynthis; cucurbitacins; cytotoxicity