title: Design and preparation of matrine surface-imprinted material and studies on its molecule recognition selectivity.
abstract: A matrine molecule surface-imprinted material was designed and prepared using an effective surface-imprinting technique developed by our group, and its molecular recognition performance and mechanism were investigated in depth. Monomer glycidyl methacrylate (GMA) was first graft-polymerized on the surfaces of micron-sized silica gel particles in surface-initiated graft polymerization manner, obtaining the grafted particles PGMA/SiO(2) with high grafting degree. Subsequently, the ring-opening reaction of the epoxy groups of the grafted macromolecules PGMA with 5-aminosalicylic acid (5-ASA) was carried out, resulting in the functional grafted particle SA-PGMA/SiO(2), on whose surfaces salicylic acid as functional group was chemically bonded. By right of the mutual strong secondary bond forces, electrostatic interaction and hydrogen bonding, SA-PGMA/SiO(2) particles produced strong adsorption for matrine. Finally, with this strong adsorption, matrine molecule surface imprinting was carried out on the surfaces of SA-PGMA/SiO(2) particles with ethylene glycol diglycidyl ether as cross-linking agent, resulting in the matrine molecule surface-imprinted material MIP-SAP/SiO(2). The binding characteristic of MIP-SAP/SiO(2) toward matrine was investigated in depth with both batch and column methods and using oxymatrine and cytisine as two contrast alkaloids. The experimental results show that MIP-SAP/SiO(2) has special recognition selectivity and excellent binding affinity for matrine. Relative to oxymatrine and cytisine, the selectivity coefficients of MIP-SAP/SiO(2) for matrine are 5.66 and 11.17, respectively.
authors.affiliation: a Department of Chemical Engineering , North University of China , Taiyuan , P.R. China.
authors.forename: Qingjuan
authors.initials: Q
authors.lastname: Lei
authors.affiliation: a Department of Chemical Engineering , North University of China , Taiyuan , P.R. China.
authors.forename: Baojiao
authors.initials: B
authors.lastname: Gao
authors.affiliation: a Department of Chemical Engineering , North University of China , Taiyuan , P.R. China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000470:Alkaloids / Q000737:chemistry*; D001392:Azocines / Q000737:chemistry; D002616:Chemical Engineering / Q000379:methods; D004852:Epoxy Compounds / Q000737:chemistry; D004853:Epoxy Resins / Q000737:chemistry; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D019804:Mesalamine / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D015394:Molecular Structure; D058105:Polymerization; D011807:Quinolizines / Q000737:chemistry*; D020156:Salicylic Acid / Q000737:chemistry; D058428:Silica Gel / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013057:Spectrum Analysis; D055672:Static Electricity; D013499:Surface Properties; D000093842:Matrines; D000093843:Quinolizidine Alkaloids
chemical_list: D000470:Alkaloids; D001392:Azocines; D004852:Epoxy Compounds; D004853:Epoxy Resins; D019804:Mesalamine; D008689:Methacrylates; D011807:Quinolizines; D020156:Salicylic Acid; D058428:Silica Gel; D012822:Silicon Dioxide; D000093842:Matrines; C035364:Quetol 651; C004712:cytisine; D000093843:Quinolizidine Alkaloids; C007870:glycidyl methacrylate
keywords: Surface imprinting; alkaloid separation; graft polymerization; matrine; molecular recognition