title: Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [(99m)Tc(MIBI)₆].
abstract: Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [(99m)Tc(MIBI)₆]⁺ as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanol:chloroform (25:75), Whatman 3MM paper and ethanol:chloroform (25:75), and the more expensive ITLC-SG and 1-propanol:chloroform (10:90) were suitable systems for the direct determination of radiochemical purity of [(99m)Tc(MIBI)₆]⁺ since impurities such as (99m)Tc-reduced-hydrolyzed (RH), (99m)TcO(4)(-) and [(99m)Tc(cysteine)₂]⁻ complex were completely separated from the radiopharmaceutical, which moved toward the front of chromatographic systems while impurities were retained at the origin. The time required for analysis was 4 to 15 min, which is appropriate for nuclear medicine routines.
authors.affiliation: Centro Translacional de Oncologia, Instituto do Câncer do Estado de São Paulo, São Paulo, SP, BR.
authors.forename: D P
authors.initials: DP
authors.lastname: Faria
authors.affiliation: Serviço de Medicina Nuclear, Departamento de Radiologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, BR.
authors.forename: C A
authors.initials: CA
authors.lastname: Buchpiguel
authors.affiliation: Serviço de Medicina Nuclear, Departamento de Radiologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, BR.
authors.forename: F L N
authors.initials: FL
authors.lastname: Marques
mesh_terms: D000438:Alcohols; D002725:Chloroform; D002845:Chromatography / Q000191:economics / Q000379:methods; D002854:Chromatography, Paper / Q000191:economics / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000191:economics / Q000379:methods*; D011786:Quality Control; D019275:Radiopharmaceuticals / Q000032:analysis* / Q000145:classification; D013670:Sodium Pertechnetate Tc 99m / Q000032:analysis*
chemical_list: D000438:Alcohols; D019275:Radiopharmaceuticals; D002725:Chloroform; D013670:Sodium Pertechnetate Tc 99m