title: The Selenylation Modification of Epimedium Polysaccharide and Isatis Root Polysaccharide and the Immune-enhancing Activity Comparison of Their Modifiers.
abstract: Epimedium polysaccharide (EPS) and isatis root polysaccharide (IRPS) were extracted, purified, and selenizingly modified by nitric acid-sodium selenite method to obtain nine selenizing EPSs (sEPSs), sEPS1-sEPS9 and nine selenizing IRPSs (sIRPSs), sIRPS1-sIRPS9, respectively. Their effects on chicken peripheral lymphocyte proliferation in vitro were compared by MTT assay. The results showed that selenium polysaccharides at appropriate concentration could promote lymphocyte proliferation more significantly than unmodified polysaccharides, sEPS5 and sIRPS5 with stronger actions were picked out and injected into the chickens vaccinated with Newcastle disease vaccine in vivo tests. The peripheral lymphocyte proliferation and serum antibody titer were determined. The results showed that sEPS5 and sIRPS5 could elevate serum antibody titer and promote lymphocyte proliferation more significantly than unmodified polysaccharides, sEPS5 possessed the strongest efficacy. These results indicate that selenylation modification can significantly enhance the immune-enhancing activity of EPS and IRPS, and sEPS5 can be as a new-type immunopotentiator of chickens.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of China.|Department of Agriculture, Dezhou University, Dezhou, 253023, People' s Republic of China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of China.
authors.forename: Ranran
authors.initials: R
authors.lastname: Hou
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of China.
authors.forename: Chanjuan
authors.initials: C
authors.lastname: Yue
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Gao
authors.affiliation: National Research Center of Veterinary Biological Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People' s Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.affiliation: National Research Center of Veterinary Biological Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People' s Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of China.
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of China.
authors.forename: Cui
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of China. ylhu@njau.edu.cn.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens / Q000276:immunology*; D034181:Epimedium / Q000737:chemistry*; D031219:Isatis / Q000737:chemistry*; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D018038:Sodium Selenite / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D011134:Polysaccharides; D018038:Sodium Selenite
keywords: Antibody titer; Epimedium polysaccharide; Isatis root polysaccharide; Lymphocyte proliferation; Selenylation modification