title: Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost-Effective Diagnosis of Infectious Disease.
abstract: A new nucleic acid detection method was developed for a rapid and cost-effective diagnosis of infectious disease. This approach relies on the three unique elements: 1) detection probes that regulate DNA polymerase activity in response to the complementary target DNA; 2) universal reporters conjugated with a single fluorophore; and 3) fluorescence polarization (FP) detection. As a proof-of-concept, the assay was used to detect and sub-type Salmonella bacteria with sensitivities down to a single bacterium in less than three hours.
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA).
authors.forename: Ki Soo
authors.initials: KS
authors.lastname: Park
authors.affiliation: Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114 (USA).|Department of Medicine, Harvard Medical School, Boston, MA 02114 (USA).
authors.forename: Richelle C
authors.initials: RC
authors.lastname: Charles
authors.affiliation: Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114 (USA).|Department of Medicine, Harvard Medical School, Boston, MA 02114 (USA).
authors.forename: Edward T
authors.initials: ET
authors.lastname: Ryan
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA). rweissleder@mgh.harvard.edu.|Department of Systems Biology, Harvard Medical School, Boston, MA 02115 (USA). rweissleder@mgh.harvard.edu.
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA). hlee@mgh.harvard.edu.
authors.forename: Hakho
authors.initials: H
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D003141:Communicable Diseases / Q000175:diagnosis*; D003362:Cost-Benefit Analysis; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques / Q000191:economics* / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D012475:Salmonella / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D009696:Nucleic Acids; D004259:DNA-Directed DNA Polymerase
keywords: DNA polymerase; aptamer; biosensors; infectious disease; nucleic acid testing