title: Astragaloside IV attenuates apoptosis of hypertrophic cardiomyocyte through inhibiting oxidative stress and calpain-1 activation.
abstract: Calpain-1 activation and oxidative stress are two critical factors contributing to apoptosis of hypertrophic cardiomyocyte. Astragaloside IV (ASIV) exhibits protective effect against various heart diseases. The present study was designed to investigate whether the inhibitory effect of ASIV on isoproterenol (ISO)-induced apoptosis of hypertrophic cardiomyocyte was associated with the anti-oxidation and calpain-1 inhibition. Hypertrophy, apoptosis, mitochondrial oxidative stress and calpain-1 expression were measured in the heart tissue of Sprague-Dawley (SD) rats and H9C2 cells treated with ISO alone or combination with ASIV. The results showed that ASIV attenuated apoptotic rate, increased Bcl-2 expression, decreased Bax expression, ameliorated the integrity of mitochondrial structure and improved mitochondrial membrane potential (MMP). Moreover, ASIV combination reduced both calpain-1 protein expression and calpain activity, down-regulated mitochondrial NOX4 (mito-NOX4) expression, increased activity of mitochondrial superoxide dismutase (mito-SOD) and mitochondrial catalase (mito-CAT) compared to ISO treated alone. The results suggested that ASIV exerted anti-apoptosis effect on ISO-induced hypertrophic cardiomyocyte by attenuating oxidative stress and calpain-1 activation.
authors.affiliation: Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Liaoning Medical University, Jinzhou, Liaoning, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Mei
authors.affiliation: Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Liaoning Medical University, Jinzhou, Liaoning, PR China.
authors.forename: Futian
authors.initials: F
authors.lastname: Tang
authors.affiliation: Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Liaoning Medical University, Jinzhou, Liaoning, PR China.
authors.forename: Meili
authors.initials: M
authors.lastname: Lu
authors.affiliation: Internal Medicine-Cardiovascular Department, The First Affiliated Hospital of Liaoning Medical University, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Liaoning Medical University, Jinzhou, Liaoning, PR China. Electronic address: hongxinwang@lnmu.edu.cn.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Human Anatomy Department of Liaoning Medical University, PR China.
authors.forename: Xuwei
authors.initials: X
authors.lastname: Hou
authors.affiliation: Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Liaoning Medical University, Jinzhou, Liaoning, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Hu
authors.affiliation: Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Liaoning Medical University, Jinzhou, Liaoning, PR China.
authors.forename: Chonghua
authors.initials: C
authors.lastname: Xu
authors.affiliation: Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Liaoning Medical University, Jinzhou, Liaoning, PR China.
authors.forename: Ronghui
authors.initials: R
authors.lastname: Han
mesh_terms: D019840:2-Propanol / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002154:Calpain / Q000378:metabolism*; D006332:Cardiomegaly / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D002316:Cardiotonic Agents / Q000494:pharmacology; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000008:administration & dosage* / Q000494:pharmacology; D014315:Triterpenes / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D002316:Cardiotonic Agents; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A; D002154:Calpain; C000606071:Capn1 protein, rat; D019840:2-Propanol
keywords: Apoptosis; Astragaloside IV; Calpain-1; Hypertrophy; Oxidation