title: Recent research progress with phospholipase C from Bacillus cereus.
abstract: Phospholipase C (PLC) catalyzes the hydrolysis of phospholipids to produce phosphate monoesters and diacylglycerol. It has many applications in the enzymatic degumming of plant oils. PLC Bc , a bacterial PLC from Bacillus cereus, is an optimal choice for this activity in terms of its wide substrate spectrum, high activity, and approved safety. Unfortunately, its large-scale production and reliable high-throughput screening of PLC Bc remain challenging. Herein, we summarize the research progress regarding PLC Bc with emphasis on the screening methods, expression systems, catalytic mechanisms and inhibitor of PLC Bc . This review hopefully will inspire new achievements in related areas, to promote the sustainable development of PLC Bc and its application.
authors.affiliation: Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, No. 38 Zheda Road, Hangzhou, 310027, People's Republic of China. 21228185@zju.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lyu
authors.affiliation: Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, No. 38 Zheda Road, Hangzhou, 310027, People's Republic of China. yelidan@zju.edu.cn.|Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang University, No. 38 Zheda Road, Hangzhou, 310027, People's Republic of China. yelidan@zju.edu.cn.
authors.forename: Lidan
authors.initials: L
authors.lastname: Ye
authors.affiliation: Wilmar (Shanghai) Biotechnology Research & Development Center Co. Ltd., No. 118 Gaodong Road, Pudong New District, Shanghai, 200137, People's Republic of China. xujunsh@wilmar.intl.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, No. 38 Zheda Road, Hangzhou, 310027, People's Republic of China. yxh-change@zju.edu.cn.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, No. 38 Zheda Road, Hangzhou, 310027, People's Republic of China. 21228184@zju.edu.cn.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, No. 38 Zheda Road, Hangzhou, 310027, People's Republic of China. yuhongwei@zju.edu.cn.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Yu
mesh_terms: D001409:Bacillus cereus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D020134:Catalytic Domain; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D013379:Substrate Specificity; D010738:Type C Phospholipases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D010738:Type C Phospholipases
keywords: Bacillus cereus; Catalytic mechanisms; Enzymatic degumming; Expression systems; Inhibitors (of phospholipase); Phospholipase C; Screening methods