title: High genetic homogeneity points to a single introduction event responsible for invasion of Cotton leaf curl Multan virus and its associated betasatellite into China.
abstract: BACKGROUND
  Cotton leaf curl Multan virus (CLCuMuV) is a Whitefly Transmitted Geminivirus (WTG) endemic to the India subcontinent and is notorious as a causal agent of cotton leaf curl disease (CLCuD), a major constraint to cotton production in south Asia. We found CLCuMuV infecting Hibiscus rosa-sinensis in Guangzhou, China in 2006. The spread and evolution of the invading CLCuMuV were monitored in the following nine years.
  
  
  RESULTS
  CLCuMuV spread rapidly in the last nine years and became established in Southern China. It infects at least five malvaceous plant species, H. rosa-sinensis, H. esculentus, Malvaiscus arboreus, Gossypium hirsutum and H. cannabinus. Complete nucleotide sequences of 34 geographically and/or temporally distinct CLCuMuV isolates were determined and analyzed together with six other publicly available genomes of CLCuMuV occurring in China. The 40 CLCuMuV isolates were found to share > 99 % nucleotide sequence identity with each other. In all cases tested, the CLCuMuVs were associated with a CLCuMuB. The 36 CLCuMuBs (30 sequenced by us) shared > 98 % nucleotide sequence identity.
  
  
  CONCLUSIONS
  The high genetic homogeneity of CLCuMuV and CLCuMuB in China suggests the establishment of them from a single founder event.
authors.affiliation: Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou, 510640, China.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Du
authors.affiliation: Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou, 510640, China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China. hezf@gdppri.com.|Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou, 510640, China. hezf@gdppri.com.
authors.forename: Zifu
authors.initials: Z
authors.lastname: He
authors.affiliation: Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou, 510640, China.
authors.forename: Xiaoman
authors.initials: X
authors.lastname: She
mesh_terms: D028483:Abelmoschus; D053839:Begomovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002681:China; D016000:Cluster Analysis; D004276:DNA, Satellite / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D016679:Genome, Viral; D019660:Malvaceae / Q000821:virology*; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D004276:DNA, Satellite; D004279:DNA, Viral