title: Identification and expression analysis of a novel stylicin antimicrobial peptide from Kuruma shrimp (Marsupenaeus japonicus).
abstract: Antimicrobial peptides (AMPs) are important components of the innate immune system and function as the first line of defense against invading pathogens. In current study we identified, cloned and characterized a novel stylicin AMP from Kuruma shrimp Marsupenaeus japonicus (Mj-sty). The full-length cDNA of Mj-sty was 428 bp with an open reading frame of 315 bp that encoded 104 amino acids. The theoretical molecular mass of mature Mj-sty was 8.693 kDa with an isoelectric point (pI) of 4.79. A proline-rich N-terminal region and a C-terminal region contained 13 cysteine residues were identified. Genomic sequence analysis with respect to its cDNA showed that Mj-sty was organized into two exons interrupted by one intron. Tissue-specific expression revealed that Mj-sty was mainly transcribed in gills and hemocytes. Expression of Mj-sty in early developmental stages demonstrated that Mj-sty mRNA were present from fertilized eggs to post-larvae of 17 days (PL17), and the expression levels showed a significant variation in different developmental stages. After challenge of white spot syndrome virus (WSSV), the time-dependent expression pattern of Mj-sty in both gills and hepatopancrease showed down-regulation at the early hours of infection, subsequently up-regulation and down-regulation, and then up-regulation at the end hours to almost the half of the controls. The results indicate that Mj-sty is potentially involved in the ontogenesis and immune responses against WSSV.
authors.affiliation: State Key Laboratory of Marine Environmental Sciences (Xiamen University), Xiamen, 361102, Fujian, China.
authors.forename: Hong-tao
authors.initials: HT
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Marine Environmental Sciences (Xiamen University), Xiamen, 361102, Fujian, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Marine Environmental Sciences (Xiamen University), Xiamen, 361102, Fujian, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Marine Environmental Sciences (Xiamen University), Xiamen, 361102, Fujian, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Marine Environmental Sciences (Xiamen University), Xiamen, 361102, Fujian, China.
authors.forename: Su-fang
authors.initials: SF
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Marine Environmental Sciences (Xiamen University), Xiamen, 361102, Fujian, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: State Key Laboratory of Marine Environmental Sciences (Xiamen University), Xiamen, 361102, Fujian, China. Electronic address: yqsu@xmu.edu.cn.
authors.forename: Yong-quan
authors.initials: YQ
authors.lastname: Su
authors.affiliation: Putian Tian-ran-xing Agricultural Development Co. Ltd., Putian, Fujian, 351100, China.
authors.forename: Chun-zhong
authors.initials: CZ
authors.lastname: Wang
authors.affiliation: Putian Tian-ran-xing Agricultural Development Co. Ltd., Putian, Fujian, 351100, China.
authors.forename: Zhi-peng
authors.initials: ZP
authors.lastname: Zheng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010063:Ovum / Q000254:growth & development / Q000378:metabolism; D033561:Penaeidae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000821:virology; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger
keywords: Antimicrobial peptide; Developmental expression; Immune response; Marsupenaeus japonicus; Stylicin; WSSV