title: Reciprocal repression between Fgf8 and miR-133 regulates cardiac induction through Bmp2 signaling.
abstract: This data article contains complementary figures and results related to the research article entitled "Negative Fgf8-Bmp2 feed-back is controlled by miR-130 during early cardiac specification" [15], which reveals what specific role miR-130 plays during the cardiac induction process. This study evidenced miR-130 a putative microRNA that targets Erk1/2 (Mapk1) 3'UTR- as a necessary linkage in the control of Fgf8 signaling, mediated by Bmp2. Thus, miR-130 regulates a negative Fgf8-Bmp2 feed-back loop responsible to achieve early cardiac specification. A significant aspect supporting our conclusions is given by the expression pattern of miR-130 during early cardiac specification, as well as by those results obtained after the designed experimental procedures. The data presented here reveal that miR-133 is also expressed within the precardiac areas during early cardiogenesis, pattern which is comparable to that of FGFR1, receptor involved in the Fgf8/ERK signaling pathway. Interestingly, our miR-133 overexpression experiments resulted in a decrease of Fgf8 expression, whereas we observed an increase of Bmp2 and subsequently of cardiac specific markers Nkx-2.5 and Gata4. Additionally, our loss-of-function experiments -through Fgf8 siRNA electroporation- showed an increase of miR-133 expression. Finally, after our Bmp2 experiments, we observed that miR-133 is upstream-regulated by Bmp2. All those results suggest that miR-133 also constitutes a crucial linkage in the crosstalk between Fgf8 and Bmp2 signaling by regulating the Fgf8/ERK pathway during cardiac induction.
authors.affiliation: Human Anatomy and Embryology, Faculty of Medicine, University of Extremadura, 06006 Badajoz, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Lopez-Sanchez
authors.affiliation: Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, CU Las Lagunillas B3-362, 23071 Jaén, Spain.
authors.forename: Diego
authors.initials: D
authors.lastname: Franco
authors.affiliation: Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, CU Las Lagunillas B3-362, 23071 Jaén, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Bonet
authors.affiliation: Pharmacy Services, Minimally Invasive Surgery Centre, Cáceres, Spain.
authors.forename: Virginio
authors.initials: V
authors.lastname: Garcia-Lopez
authors.affiliation: Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, CU Las Lagunillas B3-362, 23071 Jaén, Spain.
authors.forename: Amelia
authors.initials: A
authors.lastname: Aranega
authors.affiliation: Human Anatomy and Embryology, Faculty of Medicine, University of Extremadura, 06006 Badajoz, Spain.
authors.forename: Virginio
authors.initials: V
authors.lastname: Garcia-Martinez