title: Rhesus monkey model of liver disease reflecting clinical disease progression and hepatic gene expression analysis.
abstract: Alcoholic liver disease (ALD) is a significant public health issue with heavy medical and economic burdens. The aetiology of ALD is not yet completely understood. The development of drugs and therapies for ALD is hampered by a lack of suitable animal models that replicate both the histological and metabolic features of human ALD. Here, we characterize a rhesus monkey model of alcohol-induced liver steatosis and hepatic fibrosis that is compatible with the clinical progression of the biochemistry and pathology in humans with ALD. Microarray analysis of hepatic gene expression was conducted to identify potential molecular signatures of ALD progression. The up-regulation of expression of hepatic genes related to liver steatosis (CPT1A, FASN, LEPR, RXRA, IGFBP1, PPARGC1A and SLC2A4) was detected in our rhesus model, as was the down-regulation of such genes (CYP7A1, HMGCR, GCK and PNPLA3) and the up-regulation of expression of hepatic genes related to liver cancer (E2F1, OPCML, FZD7, IGFBP1 and LEF1). Our results demonstrate that this ALD model reflects the clinical disease progression and hepatic gene expression observed in humans. These findings will be useful for increasing the understanding of ALD pathogenesis and will benefit the development of new therapeutic procedures and pharmacological reagents for treating ALD.
authors.affiliation: Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, Chin.|National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, Chin.|National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Tan
authors.affiliation: Department of Hepatobiliary Surgery, The First People's Hospital of Yunnan Province, Kunhua Hospital Affiliated to Kunming Medical College, Kunming, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, Chin.|National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Niu
authors.affiliation: National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Yan
authors.affiliation: National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Guo
authors.affiliation: Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, Chin.|National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kang
authors.affiliation: National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
authors.forename: Yanchao
authors.initials: Y
authors.lastname: Duan
authors.affiliation: National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Chang
authors.affiliation: National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
authors.forename: Jianpeng
authors.initials: J
authors.lastname: Liao
authors.affiliation: National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
authors.forename: Chenyang
authors.initials: C
authors.lastname: Si
authors.affiliation: Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, Chin.|National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
authors.forename: Weizhi
authors.initials: W
authors.lastname: Ji
authors.affiliation: Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, Chin.|National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Si
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D004195:Disease Models, Animal; D018450:Disease Progression; D000431:Ethanol / Q000633:toxicity*; D020869:Gene Expression Profiling; D006801:Humans; D008108:Liver Diseases, Alcoholic / Q000209:etiology / Q000235:genetics* / Q000473:pathology*; D008253:Macaca mulatta; D046228:Microarray Analysis
chemical_list: D015415:Biomarkers; D000431:Ethanol