title: A New Approach to Dissect Nuclear Organization: TALE-Mediated Genome Visualization (TGV).
abstract: Spatiotemporal organization of chromatin within the nucleus has so far remained elusive. Live visualization of nuclear remodeling could be a promising approach to understand its functional relevance in genome functions and mechanisms regulating genome architecture. Recent technological advances in live imaging of chromosomes begun to explore the biological roles of the movement of the chromatin within the nucleus. Here I describe a new technique, called TALE-mediated genome visualization (TGV), which allows us to visualize endogenous repetitive sequence including centromeric, pericentromeric, and telomeric repeats in living cells.
authors.affiliation: Okazaki Institute for integrative bioscience, Okazaki, 444-8787, Japan. miyanari@nibb.ac.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Miyanari
mesh_terms: D002467:Cell Nucleus / Q000235:genetics / Q000648:ultrastructure*; D002503:Centromere / Q000235:genetics / Q000648:ultrastructure; D002843:Chromatin / Q000235:genetics; D002875:Chromosomes / Q000235:genetics / Q000648:ultrastructure*; D015894:Genome, Human; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D016615:Telomere / Q000235:genetics / Q000648:ultrastructure
chemical_list: D002843:Chromatin
keywords: Live imaging; Nuclear architecture; TALE; TGV