title: Effects of renal denervation on renal pelvic contractions and connexin expression in rats.
abstract: OBJECTIVE
  The renal pelvis shows spontaneous rhythmic contractile activity. We assessed to what extent this activity depends on renal innervation and studied the role of connexins in pelvic contractions.
  
  
  METHODS
  Rats underwent unilateral renal denervation or renal transplantation. Renal pelvic pressure and diuresis were measured in vivo. Spontaneous and agonist-induced contractions of isolated renal pelves were investigated by wire myography. Rat and human renal pelvic connexin mRNA abundances and connexin localization were studied by real-time PCR and immunofluorescence respectively.
  
  
  RESULTS
  Renal denervation or transplantation increased renal pelvic pressure in vivo by about 60 and 150%, respectively, but did not significantly affect pelvic contraction frequency. Under in vitro conditions, isolated pelvic preparations from innervated or denervated kidneys showed spontaneous contractions. Pelves from denervated kidneys showed about 50% higher contraction frequencies than pelves from innervated kidneys, whereas contraction force was similar in pelves from denervated and innervated kidneys. There was no denervation-induced supersensitivity to noradrenaline or endothelin-1. Renal denervation did not increase pelvic connexin37, 40, 43 or 45 mRNA abundances. Gap junction blockade had no effect on spontaneous pelvic contractile activity.
  
  
  CONCLUSIONS
  The denervation-induced effect on pelvic pressure may be the consequence of the enhanced diuresis. The mechanisms underlying the denervation-induced effects on pelvic contraction frequency remain unknown. Our data rule out a major role for two important candidates, by showing that renal denervation neither induced supersensitivity to contractile agonists nor increased connexin mRNA abundance in the pelvic wall.
authors.affiliation: Department of Physiology, University of Greifswald, Karlsburg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Koenen
authors.affiliation: Department of Physiology, University of Greifswald, Karlsburg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Steinbach
authors.affiliation: Department of Physiology, University of Greifswald, Karlsburg, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Schaper
authors.affiliation: Department of Urology, University of Greifswald, Greifswald, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Zimmermann
authors.affiliation: Departments of Anatomy and Cell Biology, University of Greifswald, Greifswald, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Miehe
authors.affiliation: Department of Physiology, University of Regensburg, Regensburg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Kurt
authors.affiliation: Department of Physiology, University of Greifswald, Karlsburg, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Rettig
authors.affiliation: Department of Physiology, University of Greifswald, Karlsburg, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Grisk
mesh_terms: D000818:Animals; D017630:Connexins / Q000096:biosynthesis*; D003714:Denervation; D004576:Electromyography; D005455:Fluorescent Antibody Technique; D006801:Humans; D007668:Kidney / Q000294:innervation* / Q000378:metabolism; D007682:Kidney Pelvis / Q000502:physiology*; D008297:Male; D009119:Muscle Contraction / Q000502:physiology*; D009130:Muscle, Smooth / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017630:Connexins
keywords: connexins; humans; noradrenaline; rats; renal denervation; renal nerves