title: Antimicrobial activity of a temporary sealant used in endodontic treatment: An in vitro study.
abstract: OBJECTIVE
  The present study is aimed to evaluate the antimicrobial action of Coltosol(®) in direct contact with human saliva.
  
  
  METHODS
  Twelve different individuals were selected. Saliva samples were evaluated at four different time periods: Baseline 1 (T1-initial control), T2 (2 h), T4 (24 h after contact with a standardized sample of a coronary sealer) and baseline 2 (T3-final control). Seeded plates were incubated at 37°C in a bacterial incubator for a period of 48-72 h. After the incubation period, the colony forming units were counted, and the results compared.
  
  
  RESULTS
  Differences were statistically significant. There was an inhibition of bacterial growth after the first 2 h of contact and an increase in the number of bacteria after 24 h of direct contact between the material and the saliva. Coltosol(®) presented bacterial growth inhibition in direct contact with saliva. This inhibitory effect tended to decrease over time, as shown by the two periods when the material was in contact with different samples of saliva.
  
  
  CONCLUSIONS
  The antimicrobial activity of the material is an important feature; however, other physical and chemical properties of the coronary temporary sealer should be considered.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, RJ, Brazil.
authors.forename: Helena Baruffaldi
authors.initials: HB
authors.lastname: Domingos
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, RJ, Brazil.
authors.forename: Lucio Souza
authors.initials: LS
authors.lastname: Gonçalves
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, RJ, Brazil.
authors.forename: Milton
authors.initials: M
authors.lastname: de Uzeda
keywords: Antimicrobial; dental materials; saliva