title: Lignan enhancement in hairy root cultures of Linum album using coniferaldehyde and methylenedioxycinnamic acid.
abstract: Feeding experiments with hairy root cultures of Linum album have established that the extracellular coniferaldehyde is a good precursor for production of two lignans: lariciresinol (LARI) and pinoresinol (PINO). The accumulation of the LARI, PINO, and podophyllotoxin (PTOX) in hairy roots were enhanced about 14.8-, 8.7-, and 1.5-fold (107.61, 8.7 and 6.42 µg g(-1) Fresh Wight), respectively, by the addition of coniferaldehyde (2 mM) to the culture media (after 24 hr). This result was correlated with an increase pinoresinol/lariciresinol reductase (PLR) expression gene and cinnamyl alcohol dehydrogenase (CAD) activity in the fed hairy roots. Adding 3,4-(methylendioxy)cinnamic acid (MDCA) precursor did not influence on the lignans accumulation, but the lignin content of the hairy roots was increased. Moreover, the expression genes of phenylalanine ammonialyase (PAL), CAD, and cinnamoyl-CoA reductase (CCR) were influenced after feeding hairy roots with MDCA.
authors.affiliation: a Department of Plant Biology, Faculty of Biological Sciences , Tarbiat Modares University , Tehran , Iran.
authors.forename: Najmeh
authors.initials: N
authors.lastname: Ahmadian Chashmi
authors.affiliation: a Department of Plant Biology, Faculty of Biological Sciences , Tarbiat Modares University , Tehran , Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sharifi
authors.affiliation: b Department of Genetics, Faculty of Biological Sciences , Tarbiat Modares University , Tehran , Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Behmanesh
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives* / Q000378:metabolism; D002934:Cinnamates / Q000378:metabolism*; D019597:Flax / Q000378:metabolism*; D017705:Lignans / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*
chemical_list: D002934:Cinnamates; D017705:Lignans; C075384:coniferaldehyde; C029010:cinnamic acid; D000171:Acrolein
keywords: 3,4-(methylenedioxy)cinnamic acid; Linum album; coniferaldehyde; gene expression; hairy roots; lignan