title: Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta.
abstract: The atherosclerotic mouse aorta consists of a heterogeneous population of cells, including macrophages, endothelial cells, and smooth muscle cells that play critical roles in cardiovascular disease. Identification of these vascular cells in the vessel wall is important to understanding their function in pathological conditions. Immunohistochemistry is an invaluable technique used to detect the presence of cells in different tissues. Here, we describe immunohistochemical techniques commonly used for the detection of the vascular cells in the atherosclerotic mouse aorta using cell type-specific markers.
authors.affiliation: Marc and Ruti Bell Vascular Biology Program, Division of Cardiology, Department of Medicine, New York University School of Medicine, New York, NY, 10016, USA.
authors.forename: Prashanthi
authors.initials: P
authors.lastname: Menon
authors.affiliation: Marc and Ruti Bell Vascular Biology Program, Division of Cardiology, Department of Medicine, New York University School of Medicine, New York, NY, 10016, USA. edward.fisher@nyumc.org.|The Center for the Prevention of Cardiovascular Disease, NYU School of Medicine, Smilow 7, 522 First Avenue, New York, NY, 10016, USA. edward.fisher@nyumc.org.
authors.forename: Edward A
authors.initials: EA
authors.lastname: Fisher
mesh_terms: D000818:Animals; D001011:Aorta / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D001018:Aortic Diseases / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D050197:Atherosclerosis / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry / Q000379:methods*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D032389:Myocytes, Smooth Muscle / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D058226:Plaque, Atherosclerotic
chemical_list: D015415:Biomarkers
keywords: Atherosclerosis; Endothelial cells; Immunohistochemistry; Macrophages; Mouse aorta; Smooth muscle cells