title: Phenotype Prediction of Pathogenic Nonsynonymous Single Nucleotide Polymorphisms in WFS1.
abstract: Wolfram syndrome (WS) is a rare, progressive, neurodegenerative disorder that has an autosomal recessive pattern of inheritance. The gene for WS, wolfram syndrome 1 gene (WFS1), is located on human chromosome 4p16.1 and encodes a transmembrane protein. To date, approximately 230 mutations in WFS1 have been confirmed, in which nonsynonymous single nucleotide polymorphisms (nsSNPs) are the most common forms of genetic variation. Nonetheless, there is poor knowledge on the relationship between SNP genotype and phenotype in other nsSNPs of the WFS1 gene. Here, we analysed 395 nsSNPs associated with the WFS1 gene using different computational methods and identified 20 nsSNPs to be potentially pathogenic. Furthermore, to identify the amino acid distributions and significances of pathogenic nsSNPs in the protein of WFS1, its transmembrane domain was constructed by the TMHMM server, which suggested that mutations outside of the TMhelix could have more effects on protein function. The predicted pathogenic mutations for the nsSNPs of the WFS1 gene provide an excellent guide for screening pathogenic mutations.
authors.affiliation: Department of Biotechnology, School of Basic Medical Science, Nanjing Medical University, Nanjing, P.R. China.
authors.forename: Xuli
authors.initials: X
authors.lastname: Qian
authors.affiliation: Department of Biotechnology, School of Basic Medical Science, Nanjing Medical University, Nanjing, P.R. China.
authors.forename: Luyang
authors.initials: L
authors.lastname: Qin
authors.affiliation: Department of Otolaryngology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, P.R. China.
authors.forename: Guangqian
authors.initials: G
authors.lastname: Xing
authors.affiliation: Department of Biotechnology, School of Basic Medical Science, Nanjing Medical University, Nanjing, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Cao
mesh_terms: D003198:Computer Simulation; D056726:Genetic Association Studies; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D020125:Mutation, Missense; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D017434:Protein Structure, Tertiary; D014929:Wolfram Syndrome / Q000235:genetics*
chemical_list: D008565:Membrane Proteins; C114987:wolframin protein