title: Sequential Heterotrophy-Dilution-Photoinduction Cultivation of Haematococcus pluvialis for efficient production of astaxanthin.
abstract: A novel cultivation strategy called "Sequential Heterotrophy-Dilution-Photoinduction" was successfully applied in the cultivation of Haematococcus pluvialis to produce astaxanthin effectively. Cells were first cultivated heterotrophically to achieve a high cell density, then were diluted to a suitable concentration and switched to a favorable environment for cells acclimation. Finally, the culture was transferred to high light environment for astaxanthin accumulation. By this strategy, the dry cell weight of 26 g/L and biomass productivity of 64.1mg/L/h were obtained in heterotrophy stage which surpassed ever before reported in literatures. Meanwhile, the cells could accumulate considerable astaxanthin up to 4.6% of dry cell weight after 10 days of photoinduction. Furthermore, the application prospects of the strategy were confirmed further by outdoor experiments. Therefore, this novel strategy provided a promising approach for high-efficient production of natural astaxanthin from H. pluvialis to meet the huge demand of this high value product.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Minxi
authors.initials: M
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Jiaxing Zeyuan Bio-Products Limited Company, Jiaxing 314006, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Jianke
authors.initials: J
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Fan
authors.affiliation: Jiaxing Zeyuan Bio-Products Limited Company, Jiaxing 314006, PR China.
authors.forename: Anquan
authors.initials: A
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: ygli@ecust.edu.cn.
authors.forename: Yuanguang
authors.initials: Y
authors.lastname: Li
mesh_terms: D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods; D000460:Chlorophyta / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D052836:Heterotrophic Processes; D008027:Light; D059828:Plant Cells / Q000378:metabolism; D024341:Xanthophylls / Q000096:biosynthesis
chemical_list: D024341:Xanthophylls; C005948:astaxanthine
keywords: Astaxanthin; Dilution; Haematococcus pluvialis; Heterotrophy; Photoinduction