title: The evaluation of 7 commercial real-time PCR kits for Zaire ebolavirus using virus-like particle-encapsulated EBOV RNA.
abstract: Several Chinese commercial real-time PCR kits for Zaire ebolavirus have been developed after the Ebola outbreak and used by Chinese medical teams in West Africa. In order to know the essential performance indicators of these kits, analytical sensitivity and precision were evaluated with virus-like particle (VLP)-encapsulated EBOV RNA. The limit of detection (LOD) and the precision were completed with a series of VLPs. The maximum and minimum of LOD was acquired by ZJ BioTech and Daan gene, respectively. For precision, all of the detection results were <5% except the maximum 5.17%. Among them, Puruikang, Daan gene, Sansure, ZJ BioTech, and Huada demonstrated superior reproducibility. Overall, the requirements of LOD <1000 copies/mL and coefficient of variation <5% could be satisfied by all kits except Kehua. Meanwhile, it is feasible for VLPs as a substitute of positive samples in assay evaluation.
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
authors.forename: Guojing
authors.initials: G
authors.lastname: Wang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China.
authors.forename: Yanxi
authors.initials: Y
authors.lastname: Han
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
authors.forename: Le
authors.initials: L
authors.lastname: Chang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Fifth School of Clinical Medicine, Peking University, Beijing, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Ding
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China.
authors.forename: Kuo
authors.initials: K
authors.lastname: Zhang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China.
authors.forename: Guigao
authors.initials: G
authors.lastname: Lin
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China.
authors.forename: Xie
authors.initials: X
authors.lastname: Jiehong
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital of National Health and Family Planning Commission, Beijing, People's Republic of China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China. Electronic address: jmli@nccl.org.cn.
authors.forename: Jinming
authors.initials: J
authors.lastname: Li
mesh_terms: D000354:Africa, Western; D029043:Ebolavirus / Q000302:isolation & purification*; D019142:Hemorrhagic Fever, Ebola / Q000175:diagnosis*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic
keywords: Ebola virus; Kits evaluation; Real time RT-PCR; Virus-like particles