title: Disruption of Amyloid Prion Protein Aggregates by Cationic Pyridylphenylene Dendrimers.
abstract: Disruption of amyloid protein aggregates is one of the potential therapies for treatment of neurodegenerative disorders such as prion diseases. Here, for the first time we report that pH-independent cationic pyridylphenylene dendrimers are able to disrupt amyloid protein aggregates at physiological pH as exemplified by inclusion bodies of ovine prion protein. The results show that exposure of inclusion bodies to the dendrimers leads to its partial disaggregation and release of the nanosize protein-dendrimer complexes. The complexes were characterized by SDS PAGE, DLS, and Western blotting methods. Thioflavin T fluorescence clearly demonstrated a decrease of amyloidogenic capability of the prion protein upon exposure to the dendrimers. The complexes formed are stable and do not show further aggregation.
authors.affiliation: A. N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow, Vavilova str., 28, 119991.
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Sorokina
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Leninskye gory, 1/40, 119992, Russia.
authors.forename: Yulia Yu
authors.initials: YY
authors.lastname: Stroylova
authors.affiliation: A. N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow, Vavilova str., 28, 119991. shifrina@ineos.ac.ru.
authors.forename: Zinaida B
authors.initials: ZB
authors.lastname: Shifrina
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Leninskye gory, 1/40, 119992, Russia. vimuronets@belozersky.msu.ru.
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Muronetz
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D000818:Animals; D050091:Dendrimers / Q000737:chemistry*; D011328:Prions / Q000737:chemistry*; D012756:Sheep
chemical_list: D000682:Amyloid; D050091:Dendrimers; D011328:Prions
keywords: amyloids; inclusion bodies; prion protein; protein-dendrimer complex; pyridylphenylene dendrimers