title: Schisandrae Fructus Inhibits IL-1β-Induced Matrix Metalloproteinases and Inflammatory Mediators Production in SW1353 Human Chondrocytes by Suppressing NF-κB and MAPK Activation.
abstract: Proinflammatory cytokine interleukin-1 beta (IL-1β) plays a crucial role in the pathogenesis of osteoarthritis (OA) by stimulating several mediators that contribute to cartilage degradation. Schisandrae Fructus (SF), the dried fruit of Schisandra chinensis (Turcz.) Baill. (Magnoliaceae), is widely used in traditional medicine for the treatment of a number of chronic inflammatory diseases. This study investigated the antiosteoarthritis properties of an ethanol extract of SF on IL-1β-stimulated SW1353 chondrocytes. SF attenuated IL-1β-induced expression and activity of matrix metalloproteinase (MMP)-1, MMP-3, and MMP-13 and also reduced the elevated levels of cyclooxygenase-2 and inducible nitric oxide synthase associated with the inhibition of prostaglandin E2 and nitric oxide production in IL-1β-stimulated SW1353 chondrocytes. In addition, SF markedly suppressed the nuclear translocation of nuclear factor-kappa B (NF-κB) by blocking inhibitor κB-alpha degradation and inhibited the phosphorylation of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK). These results indicate that the inhibitory effect of SF on IL-1β-stimulated expression of MMPs and inflammatory mediators production in SW1353 cells were associated with the suppression of the NF-κB and JNK/p38 MAPK signaling pathways. The results from this study indicate that SF may have therapeutic potential for the treatment of OA due to its anti-inflammatory and chondroprotective features.
authors.affiliation: Anti-Aging Research Center, Dongeui University, Busan, 614-714, Republic of Korea.|Department of Biochemistry, Dongeui University College of Korean Medicine, Busan, 614-052, Republic of Korea.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Jeong
authors.affiliation: Anti-Aging Research Center, Dongeui University, Busan, 614-714, Republic of Korea.
authors.forename: Hye Hyeon
authors.initials: HH
authors.lastname: Lee
authors.affiliation: Anti-Aging Research Center, Dongeui University, Busan, 614-714, Republic of Korea.
authors.forename: Eun-Ok
authors.initials: EO
authors.lastname: Choi
authors.affiliation: Research Institute, Bio-Port Korea INC, Marine Bio-Industry Development Center, Busan, 619-912, Republic of Korea.
authors.forename: Ki Won
authors.initials: KW
authors.lastname: Lee
authors.affiliation: Research Institute, Bio-Port Korea INC, Marine Bio-Industry Development Center, Busan, 619-912, Republic of Korea.
authors.forename: Ki Young
authors.initials: KY
authors.lastname: Kim
authors.affiliation: Research Institute, Bio-Port Korea INC, Marine Bio-Industry Development Center, Busan, 619-912, Republic of Korea.
authors.forename: Sung Goo
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Dongeui University College of Korean Medicine, Busan, 614-052, Republic of Korea.
authors.forename: Su Hyun
authors.initials: SH
authors.lastname: Hong
authors.affiliation: Department of Marine Life Sciences, Laboratory of Immunobiology, Jeju National University, Jeju, 690-756, Republic of Korea.
authors.forename: Gi-Young
authors.initials: GY
authors.lastname: Kim
authors.affiliation: Department of Molecular Biology, College of Natural Sciences and Human Ecology, Dongeui University, Busan, 614-714, Republic of Korea.
authors.forename: Cheol
authors.initials: C
authors.lastname: Park
authors.affiliation: Herbal Medicine Research Division, Herbal Medicine Resources Group, Korea Institute of Oriental Medicine, Daejeon, 305-811, Republic of Korea.
authors.forename: Ho Kyoung
authors.initials: HK
authors.lastname: Kim
authors.affiliation: Department of Horticultural Bioscience, College of Natural Resource and Life Sciences, Pusan National University, Miryang, 627-706, Republic of Korea.
authors.forename: Young Whan
authors.initials: YW
authors.lastname: Choi
authors.affiliation: Anti-Aging Research Center, Dongeui University, Busan, 614-714, Republic of Korea.|Department of Biochemistry, Dongeui University College of Korean Medicine, Busan, 614-052, Republic of Korea.
authors.forename: Yung Hyun
authors.initials: YH
authors.lastname: Choi
mesh_terms: D002460:Cell Line; D019902:Chondrocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004347:Drug Interactions; D004790:Enzyme Induction / Q000187:drug effects; D005638:Fruit / Q000737:chemistry; D006801:Humans; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D020782:Matrix Metalloproteinases / Q000096:biosynthesis* / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D032141:Schisandra / Q000737:chemistry*
chemical_list: D018836:Inflammation Mediators; D053583:Interleukin-1beta; D016328:NF-kappa B; D010936:Plant Extracts; D009569:Nitric Oxide; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D020928:Mitogen-Activated Protein Kinases; D020782:Matrix Metalloproteinases; D015232:Dinoprostone
keywords: NF-κB; Schisandra chinensis; matrix metalloproteinase; mitogen-activated protein kinases; osteoarthritis