title: Annexin A2 Acts as an Adhesion Molecule on the Endometrial Epithelium during Implantation in Mice.
abstract: To determine the function of Annexin A2 (Axna2) in mouse embryo implantation in vivo, experimental manipulation of Axna2 activities was performed in mouse endometrial tissue in vivo and in vitro. Histological examination of endometrial tissues was performed throughout the reproduction cycle and after steroid treatment. Embryo implantation was determined after blockage of the Axna2 activities by siRNA or anti-Axna2 antibody. The expression of Axna2 immunoreactivies in the endometrial luminal epithelium changed cyclically in the estrus cycle and was upregulated by estrogen. After nidatory estrogen surge, there was a concentration of Axna2 immunoreactivities at the interface between the implanting embryo and the luminal epithelium. The phenomenon was likely to be induced by the implanting embryos as no such concentration of signal was observed in the inter-implantation sites and in pseudopregnancy. Knockdown of Axna2 by siRNA reduced attachment of mouse blastocysts onto endometrial tissues in vitro. Consistently, the number of implantation sites was significantly reduced after infusion of anti-Axna2 antibody into the uterine cavity. Steroids and embryos modulate the expression of Axna2 in the endometrial epithelium. Axna2 may function as an adhesion molecule during embryo implantation in mice.
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China; Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China; Shenzhen Key Laboratory of Fertility Regulation, The University of Hong Kong-Shenzhen Hospital, Shenzhen, People's Republic of China.
authors.forename: Tian-Min
authors.initials: TM
authors.lastname: Ye
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China; Shenzhen Key Laboratory of Fertility Regulation, The University of Hong Kong-Shenzhen Hospital, Shenzhen, People's Republic of China; Centre for Reproduction, Development and Growth, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China.
authors.forename: Kai-Fai
authors.initials: KF
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China; Centre for Reproduction, Development and Growth, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China.
authors.forename: Philip C N
authors.initials: PC
authors.lastname: Chiu
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China; Centre for Reproduction, Development and Growth, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China.
authors.forename: Ronald T K
authors.initials: RT
authors.lastname: Pang
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China; Shenzhen Key Laboratory of Fertility Regulation, The University of Hong Kong-Shenzhen Hospital, Shenzhen, People's Republic of China; Centre for Reproduction, Development and Growth, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China.
authors.forename: Ernest H Y
authors.initials: EH
authors.lastname: Ng
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China; Shenzhen Key Laboratory of Fertility Regulation, The University of Hong Kong-Shenzhen Hospital, Shenzhen, People's Republic of China; Centre for Reproduction, Development and Growth, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China.
authors.forename: William S B
authors.initials: WS
authors.lastname: Yeung
mesh_terms: D000818:Animals; D017306:Annexin A2 / Q000378:metabolism*; D001755:Blastocyst / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D010064:Embryo Implantation / Q000502:physiology*; D004717:Endometrium / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011555:Pseudopregnancy / Q000378:metabolism
chemical_list: D017306:Annexin A2; D015815:Cell Adhesion Molecules; D004967:Estrogens