title: Microfilarial periodicity of Wuchereria bancrofti in Assam, Northeast India.
abstract: OBJECTIVE
  Wuchereria bancrofti has shown nocturnal periodicity in India and other endemic countries of the world except pacific regions where non-periodic or diurnal sub-periodic forms have been reported. Presence of sub-periodic form of W. bancrofti in Andaman and Nicobar Islands, India and a case report of sub- periodic form of W. bancrofti from Mysore, India provide basis for exploring the periodicity pattern of microfilaria of W. bancrofti prevalent in Assam, Northeastern region of India. State of Assam has unique geographical location as its Northeastern region shares international boundaries with Nepal, China, Bhutan, Myanmar and Bangladesh. Evolutionary association of W. bancrofti found in Assam is not known and possibility of its link with W. bancrofti form, prevalent in neighbouring countries may not be ruled out. Hence, this study was undertaken to know the microfilarial periodicity of W. bancrofti in Assam.
  
  
  METHODS
  Ten microfilaria positive adult male individuals having moderate to high microfilaraemia were selected. Informed written consent from each participant was obtained. The presence of microfilaria was observed at two hourly intervals over a period of 24 h using 50 μl of finger prick peripheral blood samples. Peripheral blood smears were processed, stained and examined under microscope and microfilaria counts were recorded.
  
  
  RESULTS
  Data collected were calculated and analyzed using modified statistical method, and the periodicity curve was prepared. Typical nocturnal periodicity was observed at a peak time of 0003 hrs with a periodicity index of 136.2.
  
  
  CONCLUSIONS
  Analysis of the data revealed nocturnal periodicity of the W. bancrofti prevalent in the Assam with peak periodicity about one hour ahead of the other states in India. Findings will be helpful in evaluation and monitoring of ongoing MDA programme for elimination of LF in Assam.
authors.affiliation: Regional Medical Research Centre (ICMR), Northeastern Region, Dibrugarh, India.
authors.forename: A M
authors.initials: AM
authors.lastname: Khan
authors.forename: P
authors.initials: P
authors.lastname: Dutta
authors.forename: S
authors.initials: S
authors.lastname: Das
authors.forename: A K
authors.initials: AK
authors.lastname: Pathak
authors.forename: P
authors.initials: P
authors.lastname: Sarmah
authors.forename: M E
authors.initials: ME
authors.lastname: Hussain
authors.forename: J
authors.initials: J
authors.lastname: Mahanta
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001769:Blood / Q000469:parasitology*; D004605:Elephantiasis, Filarial / Q000469:parasitology; D006801:Humans; D007194:India; D008297:Male; D008853:Microscopy; D059208:Parasite Load; D010507:Periodicity; D014958:Wuchereria bancrofti / Q000302:isolation & purification*; D055815:Young Adult