title: The Overexpression of FEN1 and RAD54B May Act as Independent Prognostic Factors of Lung Adenocarcinoma.
abstract: Synthetic lethality arises when a combination of mutations in two or more genes leads to cell death. However, the prognostic role of concordant overexpression of synthetic lethality genes in protein level rather than a combination of mutations is not clear. In this study, we explore the prognostic role of combined overexpression of paired genes in lung adenocarcinoma. We used immunohistochemical staining to investigate 24 paired genes in 93 lung adenocarcinoma patients and Kaplan-Meier analysis and Cox proportional hazards models to evaluate their prognostic roles. Among 24 paired genes, only FEN1 (Flap endonuclease 1) and RAD54B (RAD54 homolog B) were overexpressed in lung adenocarcinoma patients with poor prognosis. Patients with expression of both FEN1 and RAD54B were prone to have advanced nodal involvement and significantly poor prognosis (HR = 2.35, P = 0.0230). These results suggest that intensive follow up and targeted therapy might improve clinical outcome for patients who show expression of both FEN1 and RAD54B.
authors.affiliation: Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Pathology, Lin Shin Hospital, Taichung, Taiwan.
authors.forename: Jau-Chung
authors.initials: JC
authors.lastname: Hwang
authors.affiliation: School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Medical Education, Chung Shan Medical University Hospital, Taichung, Taiwan; Department of Medical Technology, Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli, Taiwan.
authors.forename: Wen-Wei
authors.initials: WW
authors.lastname: Sung
authors.affiliation: Department of Public Health and Environmental Medicine, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Hung-Pin
authors.initials: HP
authors.lastname: Tu
authors.affiliation: Division of Thoracic Surgery, Department of Surgery, E-Da Hospital, Kaohsiung, Taiwan.
authors.forename: Kun-Chou
authors.initials: KC
authors.lastname: Hsieh
authors.affiliation: Department of Surgical Pathology, Changhua Christian Hospital, Changhua, Taiwan.
authors.forename: Chung-Min
authors.initials: CM
authors.lastname: Yeh
authors.affiliation: School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Medical Technology, Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli, Taiwan; Department of Surgical Pathology, Changhua Christian Hospital, Changhua, Taiwan.
authors.forename: Chih-Jung
authors.initials: CJ
authors.lastname: Chen
authors.affiliation: Department of Surgical Pathology, Changhua Christian Hospital, Changhua, Taiwan.
authors.forename: Hui-Chun
authors.initials: HC
authors.lastname: Tai
authors.affiliation: Department of Pathology, E-Da Hospital and I-SHOU University, Kaohsiung, Taiwan.
authors.forename: Chao-Tien
authors.initials: CT
authors.lastname: Hsu
authors.affiliation: Institute of Statistical Science, Academia Sinica, Taipei, Taiwan.
authors.forename: Grace S
authors.initials: GS
authors.lastname: Shieh
authors.affiliation: Department of Laboratory Medicine, and Center of RNA Biology and Clinical Application, China Medical University Hospital, China Medical University, Taichung, Taiwan.
authors.forename: Jan-Gowth
authors.initials: JG
authors.lastname: Chang
authors.affiliation: School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Surgical Pathology, Changhua Christian Hospital, Changhua, Taiwan.
authors.forename: Kun-Tu
authors.initials: KT
authors.lastname: Yeh
authors.affiliation: Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Division of Hematology/Oncology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
authors.forename: Ta-Chih
authors.initials: TC
authors.lastname: Liu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D004265:DNA Helicases / Q000235:genetics*; D005260:Female; D045585:Flap Endonucleases / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics*; D011379:Prognosis
chemical_list: D009687:Nuclear Proteins; C491221:RAD54B protein, human; D045585:Flap Endonucleases; C468317:FEN1 protein, human; D004265:DNA Helicases