title: The Production of High Purity Phycocyanin by Spirulina platensis Using Light-Emitting Diodes Based Two-Stage Cultivation.
abstract: Phycocyanin is a photosynthetic pigment found in photosynthetic cyanobacteria, cryptophytes, and red algae. In general, production of phycocyanin depends mainly on the light conditions during the cultivation period, and purification of phycocyanin requires expensive and complex procedures. In this study, we propose a new two-stage cultivation method to maximize the quantitative content and purity of phycocyanin obtained from Spirulina platensis using red and blue light-emitting diodes (LEDs) under different light intensities. In the first stage, Spirulina was cultured under a combination of red and blue LEDs to obtain the fast growth rate until reaching an absorbance of 1.4-1.6 at 680 nm. Next, blue LEDs were used to enhance the concentration and purity of the phycocyanin in Spirulina. Two weeks of the two-stage cultivation of Spirulina yielded 1.28 mg mL(-1) phycocyanin with the purity of 2.7 (OD620/OD280).
authors.affiliation: Department of Interdisciplinary Program of Integrated Biotechnology, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul, 121-742, Republic of Korea.
authors.forename: Sang-Hyo
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Mechanical Engineering, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul, 121-742, Republic of Korea.
authors.forename: Ju Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: Department of Interdisciplinary Program of Integrated Biotechnology, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul, 121-742, Republic of Korea.
authors.forename: Yoori
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Interdisciplinary Program of Integrated Biotechnology, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul, 121-742, Republic of Korea. sylee@sogang.ac.kr.|Department of Mechanical Engineering, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul, 121-742, Republic of Korea. sylee@sogang.ac.kr.
authors.forename: Seung-Yop
authors.initials: SY
authors.lastname: Lee
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D008027:Light; D010788:Photosynthesis; D010798:Phycocyanin / Q000096:biosynthesis* / Q000302:isolation & purification; D053545:Spirulina / Q000378:metabolism*
chemical_list: D010798:Phycocyanin
keywords: Cultivation; Light intensity; Light-emitting diode (LED); Phycocyanin; Spirulina platensis; Wavelength