title: Transplantation of Tissue-Engineered Cartilage in an Animal Model (Xenograft and Autograft): Construct Validation.
abstract: Tissue engineering holds great promise for cartilage repair with minimal donor-site morbidity. The in vivo maturation of a tissue-engineered construct can be tested in the subcutaneous tissues of the same species for autografts or of immunocompromised animals for allografts or xenografts. This section describes detailed protocols for the surgical transplantation of a tissue-engineered construct into an animal model to assess construct validity.
authors.affiliation: Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, School of Medicine, University of California, La Jolla, San Diego, CA, USA.|Department of Plastic and Reconstructive Surgery, Showa University Fujigaoka Hospital, Yokohama, Kanagawa, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nemoto
authors.affiliation: Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, School of Medicine, University of California, La Jolla, San Diego, CA, USA.
authors.forename: Deborah
authors.initials: D
authors.lastname: Watson
authors.affiliation: Department of Orthopaedic Surgery, School of Medicine, University of California, 9500 Gilman Dr., Mail Code 0863, La Jolla, San Diego, CA, USA. koichimasuda@ucsd.edu.
authors.forename: Koichi
authors.initials: K
authors.lastname: Masuda
mesh_terms: D000818:Animals; D064592:Autografts; D002356:Cartilage / Q000166:cytology / Q000276:immunology / Q000378:metabolism / Q000637:transplantation*; D019902:Chondrocytes / Q000276:immunology / Q000378:metabolism / Q000637:transplantation*; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control; D006085:Graft Survival; D064593:Heterografts; D016867:Immunocompromised Host; D020007:Implants, Experimental / Q000009:adverse effects; D008819:Mice, Nude; D023421:Models, Animal; D011817:Rabbits; D044968:Regenerative Medicine / Q000379:methods*; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*
keywords: Alginate-recovered-chondrocyte; Animal model; Autograft; Cartilage repair; Mouse; Rabbit; Tissue engineering; Transplantation; Xenograft