title: Bioinformatics Prediction and In Vitro Analysis Revealed That miR-17 Targets Cyclin D1 mRNA in Triple Negative Breast Cancer Cells.
abstract: Breast cancer is one of the most prevalent malignancies among women worldwide. Triple negative breast cancer (TNBC) is a type of breast cancer in which estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 (HER-2) are not expressed. There is no targeted therapy for this type of cancer, and available therapies have poor therapeutic effects. Performing a preliminary research, we selected cyclin D1 (CCND1) gene of Wnt signaling pathway which is a target of miRNAs, a promising set of biomolecules in diagnosis and treatment of breast cancer. In this study using bioinformatic analyses, miR-17 was selected as it targets the 3'UTR of CCND1 gene with the highest score. Luciferase assay results also confirmed the bioinformatic prediction. Decreased expression of miR-17 in MDA-MB-231 cell line was observed using qRT-PCR method. After lentiviral transduction of miR-17 to the target cells, gene expression analysis showed decreased expression of CCND1 gene. We found miR-17 as an attractive molecule that after intensive research can probably be used as a biomarker in TNBC.
authors.affiliation: Tehran Medical Branch, Islamic Azad University, khaghani st, shariati Ave, PO Box 19395/1495, Tehran, Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Karami
authors.affiliation: Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Velenjak, Shahid Chamran Freeway, Tehran, Iran.|Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Taleghani Hospital, Velenjak, Shahid Chamran Freeway, Tehran, Iran.
authors.forename: Samira
authors.initials: S
authors.lastname: Mohammadi-Yeganeh
authors.affiliation: Tehran Medical Branch, Islamic Azad University, khaghani st, shariati Ave, PO Box 19395/1495, Tehran, Iran.
authors.forename: Nairi
authors.initials: N
authors.lastname: Abedi
authors.affiliation: Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Taleghani Hospital, Velenjak, Shahid Chamran Freeway, Tehran, Iran.
authors.forename: Ameneh
authors.initials: A
authors.lastname: Koochaki
authors.affiliation: Department of Medical Biotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Azadi Sq., Jomhoori Slami Blv., Zanjan, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Kia
authors.affiliation: Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, 25th km of Tehran-karaj highway, PO Box 3159915111, Tehran, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Paryan
mesh_terms: D019295:Computational Biology; D019938:Cyclin D1 / Q000235:genetics*; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D066298:In Vitro Techniques; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D064726:Triple Negative Breast Neoplasms / Q000235:genetics*
chemical_list: C524941:MIRN17 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D019938:Cyclin D1
keywords: CCND1 gene; luciferase assay; miRNA; quantitative real-time RT-PCR; triple negative breast cancer