title: Experimental autoimmune encephalomyelitis and age-related correlations of NADPH oxidase, MMP-9, and cell adhesion molecules: The increased disease severity and blood-brain barrier permeability in middle-aged mice.
abstract: The aim of the present study was to investigate effect of two different ages (6 weeks [6 W] vs. 6 months [6 M]) on blood-brain barrier (BBB) disruption in EAE and evaluate the expression and correlations of NADPH oxidase, MMP-9, ICAM-1, and VCAM-1 following increased age and EAE induction. Higher disease severity was observed in 6 M-EAE than 6 W-EAE. The four factors were significantly elevated and correlated in 6 M-EAE. BBB permeability increased with statistically significant interaction between age and EAE effects. We suggest strong correlations between NADPH oxidase and the other factors play important roles in increased BBB disruption and EAE susceptibility in middle-aged mice.
authors.affiliation: Toxicology Laboratory, Doping Control Center, Korea Institute of Science and Technology, Seoul 136 791, Republic of Korea; Department of Biological Chemistry, University of Science and Technology, Daejeon 305 350, Republic of Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Seo
authors.affiliation: Toxicology Laboratory, Doping Control Center, Korea Institute of Science and Technology, Seoul 136 791, Republic of Korea; Department of Biological Chemistry, University of Science and Technology, Daejeon 305 350, Republic of Korea.
authors.forename: Mahbub
authors.initials: M
authors.lastname: Hasan
authors.affiliation: Toxicology Laboratory, Doping Control Center, Korea Institute of Science and Technology, Seoul 136 791, Republic of Korea; Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul 130 701, Republic of Korea.
authors.forename: Joon-Seung
authors.initials: JS
authors.lastname: Han
authors.affiliation: Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul 136 791, Republic of Korea; Department of Biological Chemistry, University of Science and Technology, Daejeon 305 350, Republic of Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kang
authors.affiliation: Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul 136 791, Republic of Korea; Department of Biological Chemistry, University of Science and Technology, Daejeon 305 350, Republic of Korea.
authors.forename: Byung-Hwa
authors.initials: BH
authors.lastname: Jung
authors.affiliation: Division of Rheumatology, Department of Internal Medicine, College of Medicine, Catholic University of Korea, Seoul 137 701, Republic of Korea.
authors.forename: Seung-Ki
authors.initials: SK
authors.lastname: Kwok
authors.affiliation: Division of Rheumatology, Department of Internal Medicine, Konkuk University Medical Center, Seoul 143 729, Republic of Korea.
authors.forename: Ho-Youn
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Toxicology Laboratory, Doping Control Center, Korea Institute of Science and Technology, Seoul 136 791, Republic of Korea; Department of Biological Chemistry, University of Science and Technology, Daejeon 305 350, Republic of Korea. Electronic address: oskwon@kist.re.kr.
authors.forename: Oh-Seung
authors.initials: OS
authors.lastname: Kwon
mesh_terms: D000375:Aging; D000818:Animals; D001812:Blood-Brain Barrier / Q000503:physiopathology*; D001835:Body Weight; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005620:Freund's Adjuvant / Q000633:toxicity; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000276:immunology; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology / Q000633:toxicity; D019255:NADPH Oxidases / Q000378:metabolism*; D010446:Peptide Fragments / Q000276:immunology / Q000633:toxicity; D010539:Permeability; D037342:Pertussis Toxin / Q000633:toxicity; D013997:Time Factors
chemical_list: D015815:Cell Adhesion Molecules; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; C445081:myelin oligodendrocyte glycoprotein (35-55); D005620:Freund's Adjuvant; D019255:NADPH Oxidases; D037342:Pertussis Toxin; D020780:Matrix Metalloproteinase 9
keywords: Adhesion molecules; Age; Blood–brain barrier; Experimental autoimmune encephalomyelitis; MMP-9; NADPH oxidase