title: Quantitative determination of uric acid using CdTe nanoparticles as fluorescence probes.
abstract: A convenient enzymatic optical method for uric acid detection was developed based on the fluorescence quenching of ligand-capped CdTe nanoparticles by H2O2 which was generated from the enzymatic reaction of uric acid. The interactions between the CdTe nanoparticles capped with different ligands (glutathione, 3-mercaptopropionic acid, and thioglycerol) and H2O2 were investigated. The fluorescence quenching studies of GSH-capped CdTe nanoparticles demonstrated an excellent sensitivity to H2O2. The effects of uric acid, uricase and H2O2 on the fluorescence intensity of CdTe nanoparticles were also explored. The detection conditions, reaction time, pH value, incubation period and the concentration of uricase and uric acid were optimized. The detection limit of uric acid was found to be 0.10 µM and the linear range was 0.22-6 µM under the optimized experimental conditions. These results typify that CdTe nanoparticles could be used as a fluorescent probe for uric acid detection.
authors.affiliation: Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular (Yanbian University), Ministry of Education, Yanji 133002, China.
authors.forename: Dongri
authors.initials: D
authors.lastname: Jin
authors.affiliation: Anastro Laboratory, Department of Chemistry, Changwon National University, Changwon 641-773, Republic of Korea.
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Seo
authors.affiliation: Anastro Laboratory, Department of Chemistry, Changwon National University, Changwon 641-773, Republic of Korea; NhaTrang Institute of Technology and Research Application, VAST, 2 Hungvuong, Nhatrang, Vietnam. Electronic address: buithehuy.nt@gmail.com.
authors.forename: Bui The
authors.initials: BT
authors.lastname: Huy
authors.affiliation: Anastro Laboratory, Department of Chemistry, Changwon National University, Changwon 641-773, Republic of Korea.
authors.forename: Quoc-Thai
authors.initials: QT
authors.lastname: Pham
authors.affiliation: Anastro Laboratory, Department of Chemistry, Changwon National University, Changwon 641-773, Republic of Korea.
authors.forename: Maxwell L
authors.initials: ML
authors.lastname: Conte
authors.affiliation: Anastro Laboratory, Department of Chemistry, Changwon National University, Changwon 641-773, Republic of Korea.
authors.forename: Daniel
authors.initials: D
authors.lastname: Thangadurai
authors.affiliation: Anastro Laboratory, Department of Chemistry, Changwon National University, Changwon 641-773, Republic of Korea. Electronic address: yilee@changwon.ac.kr.
authors.forename: Yong-Ill
authors.initials: YI
authors.lastname: Lee
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009195:Peroxidase / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013691:Tellurium / Q000737:chemistry*; D014503:Urate Oxidase / Q000737:chemistry*; D014527:Uric Acid / Q000652:urine*
chemical_list: D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D014527:Uric Acid; D009195:Peroxidase; D014503:Urate Oxidase; D013691:Tellurium; C028337:cadmium telluride
keywords: Enzyme; Fluorescence quenching; Hydrogen peroxide; Nanoparticles; Uric acid