title: QUANTITATIVE VS. CONVENTIONAL PCR FOR DETECTION OF HUMAN ADENOVIRUSES IN WATER AND SEDIMENT SAMPLES.
abstract: Human Adenoviruses (HAdV) are notably resistant in the environment. These agents may serve as effective indicators of fecal contamination, and may act as causative agents of a number of different diseases in human beings. Conventional polymerase chain reaction (PCR) and, more recently, quantitative PCR (qPCR) are widely used for detection of viral agents in environmental matrices. In the present study PCR and SYBR(r)Green qPCR assays were compared for detection of HAdV in water (55) and sediments (20) samples of spring and artesian wells, ponds and streams, collected from dairy farms. By the quantitative methodology HAdV were detected in 87.3% of the water samples and 80% of the sediments, while by the conventional PCR 47.3% and 35% were detected in water samples and sediments, respectively.
authors.affiliation: Universidade Feevale, Novo Hamburgo, RS, BR.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Staggemeier
authors.affiliation: Universidade Feevale, Novo Hamburgo, RS, BR.
authors.forename: Marina
authors.initials: M
authors.lastname: Bortoluzzi
authors.affiliation: Universidade Feevale, Novo Hamburgo, RS, BR.
authors.forename: Tatiana Moraes da Silva
authors.initials: TM
authors.lastname: Heck
authors.affiliation: Universidade Feevale, Novo Hamburgo, RS, BR.
authors.forename: Fernando Rosado
authors.initials: FR
authors.lastname: Spilki
authors.affiliation: Universidade Feevale, Novo Hamburgo, RS, BR.
authors.forename: Sabrina Esteves de Matos
authors.initials: SE
authors.lastname: Almeida
mesh_terms: D000260:Adenoviruses, Human / Q000302:isolation & purification*; D004784:Environmental Monitoring; D019015:Geologic Sediments / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014871:Water Microbiology