title: Pomegranate Seed Oil Exerts Synergistic Effects with trans-Resveratrol in a Self-nanoemulsifying Drug Delivery System.
abstract: Pomegranate seed oil (PSO) has diverse bioactivities. It was hyphothesized that if PSO were employed to construct a trans-resveratrol-loaded self-nanoemulsifying drug delivery system (RES SNEDDS-PSO), not only could PSO serve as an oil phase but also exert synergistic effects with resveratrol to yield better therapeutic outcomes. In this study, we prepared RES SNEDDS-PSO for the first time to validate that hypothesis. The anti-inflammatory and anticancer activities of RES SNEDDS-PSO were compared with another SNEDDS composed of oil phase isopropyl palmitate (RES SNEDDS-IP). The results showed that upon exposure to a 10-fold amount of water, RES SNEDDS-PSO was converted into nanoemulsions with a mean size of 44 nm. Nanoemulsions enhanced the water solubility of resveratrol by 20-fold, significantly improved resveratrol stability in intestinal fluid, and slowed the decomposition of resveratrol in water by 1-fold. An in vivo anti-infection test showed that the degree of inflammatory swelling in mice given RES SNEDDS-PSO was only 60 and 76% that of the group fed with RES SNEDDS-IP at doses of 10 and 20 mg/kg, respectively. An in vitro anticancer study showed that the inhibitory rate of RES SNEDDS-PSO against MCF-7 breast cancer cells was 2.03- and 1.24-fold that of RES SNEDDS-IP at a concentration of 12.5 and 25 µg/mL, respectively. This study demonstrated that the newly developed SNEDDS may be a prospective formulation in the functional food and clinical fields.
authors.affiliation: College of Chemistry & Environment Protection Engineering, Southwest University for Nationalities.
authors.forename: Lu-yang
authors.initials: LY
authors.lastname: Lu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhi-feng
authors.initials: ZF
authors.lastname: Zhang
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Gou
authors.forename: Jun-hao
authors.initials: JH
authors.lastname: Jiang
authors.forename: Ji-zhong
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Yao
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D002351:Carrageenan; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems; D004357:Drug Synergism; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005750:Gastric Juice / Q000737:chemistry; D006801:Humans; D007419:Intestinal Secretions / Q000737:chemistry; D029561:Lythraceae; D061986:MCF-7 Cells; D051379:Mice; D010938:Plant Oils / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D000077185:Resveratrol; D012639:Seeds; D012995:Solubility; D013267:Stilbenes / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D014867:Water / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D010938:Plant Oils; D013267:Stilbenes; D014867:Water; D002351:Carrageenan; D000077185:Resveratrol