title: Preparative isolation and purification of xanthohumol from hops (Humulus lupulus L.) by high-speed counter-current chromatography.
abstract: Xanthohumol (XN) and related prenylflavonoids are the main bioactive components of hops (Humulus lupulus L.). The current work is to investigate the use of high-speed counter-current chromatography (HSCCC) in search for high isolation of xanthohumol from hops. A solvent system consisted of n-hexane-ethyl acetate-methanol-water at a volume ratio of 5:5:4:3 was employed. The results demonstrated that the constructed method could be well applied for the isolation of xanthohumol from hops extract. After HSCCC isolation procedure, the purity of xanthohumol was over 95% assayed by HPLC and the yield of extraction was 93.60%. The chemical structure identification of xanthohumol was carried out by UV, (1)H NMR and (13)C NMR. The present results demonstrated that xanthohumol could be efficiently obtained using a single HSCCC step from H. lupulus L. extract.
authors.affiliation: Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China. Electronic address: chenqh@zju.edu.cn.
authors.forename: Qi-He
authors.initials: QH
authors.lastname: Chen
authors.affiliation: Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ming-Liang
authors.initials: ML
authors.lastname: Fu
authors.affiliation: Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China.
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Chen
authors.affiliation: Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Liu
authors.affiliation: Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China.
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: He
authors.affiliation: Xiaoshan College of Science and Technology, Zhejiang Ocean University, Hangzhou 311200, China. Electronic address: pu2002@126.com.
authors.forename: Shou-Cheng
authors.initials: SC
authors.lastname: Pu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry*; D027582:Humulus / Q000737:chemistry*; D015394:Molecular Structure; D011427:Propiophenones / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D011427:Propiophenones; C104536:xanthohumol
keywords: High-speed counter-current chromatography (HSCCC); Humulus lupulus; Separation and purification; Xanthohumol