title: Transgenerational effects of 17α-ethinyl estradiol on anxiety behavior in the guppy, Poecilia reticulata.
abstract: Environmental contaminants can cause alterations that can be transgenerationally transmitted to subsequent generations. Estrogens are among those contaminants shown to induce heritable changes that persist over generations in mammals. Results in other vertebrates are few. We have analyzed the effects on anxiety of 17α-ethinyl estradiol (EE2) in the F1 and F2 generations in guppies, Poecilia reticulata, obtained from F0 fish maternally exposed to 0 or 20ng/L EE2 until birth. F0 males and females were bred with fish of the same treatment but different families producing F1 offspring. Behavior in the novel tank test at 6months revealed that males with EE2-exposed parents had significantly longer latency to the upper half of the tank than control males, while no EE2 effects were observed in females. Also in F2, obtained from F1 as above, males in the EE2 group had longer latency time compared to control males, with no differences due to EE2-exposure of F0 observed in females. In the scototaxis (light/dark preference) test, latency to first transition to black compartment and total transitions to black were significantly altered in females due to EE2 exposure of F0 while the total time in black was higher in males with EE2-exposed F0 compared with controls. The increased anxiety in the F2 generation demonstrates a transgenerational anxiety phenotype and shows that non-reproductive behavior can be transgenerationally modified by estrogens in fish.
authors.affiliation: School of Natural Sciences, Technology and Environmental Studies, Södertörn University, SE-141 86 Huddinge, Sweden; Örebro Life Science Centre, School of Science and Technology, Örebro University, SE-170 82 Örebro, Sweden.
authors.forename: Kristina
authors.initials: K
authors.lastname: Volkova
authors.affiliation: School of Natural Sciences, Technology and Environmental Studies, Södertörn University, SE-141 86 Huddinge, Sweden; Örebro Life Science Centre, School of Science and Technology, Örebro University, SE-170 82 Örebro, Sweden.
authors.forename: Nasim
authors.initials: N
authors.lastname: Reyhanian Caspillo
authors.affiliation: School of Natural Sciences, Technology and Environmental Studies, Södertörn University, SE-141 86 Huddinge, Sweden.
authors.forename: Tove
authors.initials: T
authors.lastname: Porseryd
authors.affiliation: School of Natural Sciences, Technology and Environmental Studies, Södertörn University, SE-141 86 Huddinge, Sweden; Department of Organism Biology, Uppsala University, SE-75 236 Uppsala, Sweden.
authors.forename: Stefan
authors.initials: S
authors.lastname: Hallgren
authors.affiliation: School of Natural Sciences, Technology and Environmental Studies, Södertörn University, SE-141 86 Huddinge, Sweden.
authors.forename: Patrik
authors.initials: P
authors.lastname: Dinnetz
authors.affiliation: School of Natural Sciences, Technology and Environmental Studies, Södertörn University, SE-141 86 Huddinge, Sweden.
authors.forename: Håkan
authors.initials: H
authors.lastname: Olsén
authors.affiliation: School of Natural Sciences, Technology and Environmental Studies, Södertörn University, SE-141 86 Huddinge, Sweden. Electronic address: inger.porsch-hallstrom@sh.se.
authors.forename: Inger
authors.initials: I
authors.lastname: Porsch Hällström
mesh_terms: D000818:Animals; D001007:Anxiety / Q000139:chemically induced*; D001522:Behavior, Animal / Q000187:drug effects*; D004967:Estrogens / Q000494:pharmacology*; D004997:Ethinyl Estradiol / Q000494:pharmacology*; D005260:Female; D008297:Male; D011036:Poecilia; D012737:Sex Factors
chemical_list: D004967:Estrogens; D004997:Ethinyl Estradiol
keywords: 17α-Ethinyl estradiol; Behavior; EDCs; Fish; Novel tank; Scototaxis; Transgenerational effects