title: Imaging normal and cancerous human gastric muscular layer in transverse and longitudinal sections by multiphoton microscopy.
abstract: Multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) has been widely used for imaging microstructure of biological tissues. In this article, we used MPM to investigate the microstructure changes of normal and cancerous human gastric muscular layer in transverse and longitudinal sections. The results displayed different patterns of microstructure changes of smooth muscular tissue, cell morphology and interstitial fibers in transverse and longitudinal sections, being similar to standard histopathological images but without the need for tissue processing. Our study demonstrated that MPM can bring more detailed complementary information on tissue architecture through observing transverse and longitudinal sections of tissues, which are the important pathological information when the pathologists diagnose the gastrointestinal lesions. These observations indicate that MPM could be an important potential tool to provide real-time pathological diagnosis for gastric cancer in the future. SCANNING 38:357-364, 2016. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Pathology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Deyong
authors.initials: D
authors.lastname: Kang
authors.affiliation: Department of Gastric Surgery, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Zhenrong
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China.
authors.forename: Lianhuang
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China.
authors.forename: Shuangmu
authors.initials: S
authors.lastname: Zhuo
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Gastric Surgery, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Chen
mesh_terms: D041981:Gastrointestinal Tract / Q000166:cytology*; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton; D009130:Muscle, Smooth / Q000648:ultrastructure*; D009369:Neoplasms; D013274:Stomach Neoplasms / Q000473:pathology
keywords: gastric muscular layer; multiphoton microscopy; second harmonic generation (SHG); two-photon excited fluorescence (TPEF)