title: MicroRNA-212 negatively regulates starvation induced autophagy in prostate cancer cells by inhibiting SIRT1 and is a modulator of angiogenesis and cellular senescence.
abstract: Among a number of non-coding RNAs, role of microRNAs (miRNAs) in cancer cell proliferation, cancer initiation, development and metastasis have been extensively studied and miRNA based therapeutic approaches are being pursued. Prostate cancer (PCa) is a major health concern and several deregulated miRNAs have been described in PCa. miR-212 is differentially modulated in multiple cancers however its function remains elusive. In this study, we found that miR-212 is downregulated in PCa tissues when compared with benign adjacent regions (n = 40). Also, we observed reduced levels of circulatory miR-212 in serum from PCa patients (n = 40) when compared with healthy controls (n = 32). Elucidating the functional role of miR-212, we demonstrate that miR-212 negatively modulates starvation induced autophagy in PCa cells by targeting sirtuin 1 (SIRT1). Overexpression of miR-212 also leads to inhibition of angiogenesis and cellular senescence. In conclusion, our study indicates a functional role of miR-212 in PCa and suggests the development of miR-212 based therapies.
authors.affiliation: Cancer Research Laboratory, Division of Science and Mathematics, University of the District of Columbia, Washington, DC, USA.
authors.forename: Malathi
authors.initials: M
authors.lastname: Ramalinga
authors.affiliation: Cancer Research Laboratory, Division of Science and Mathematics, University of the District of Columbia, Washington, DC, USA.
authors.forename: Arpita
authors.initials: A
authors.lastname: Roy
authors.affiliation: Cancer Research Laboratory, Division of Science and Mathematics, University of the District of Columbia, Washington, DC, USA.
authors.forename: Anvesha
authors.initials: A
authors.lastname: Srivastava
authors.affiliation: Cancer Research Laboratory, Division of Science and Mathematics, University of the District of Columbia, Washington, DC, USA.
authors.forename: Asmita
authors.initials: A
authors.lastname: Bhattarai
authors.affiliation: Groton School, Groton, MA, USA.
authors.forename: Varsha
authors.initials: V
authors.lastname: Harish
authors.affiliation: Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
authors.forename: Simeng
authors.initials: S
authors.lastname: Suy
authors.affiliation: Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
authors.forename: Sean
authors.initials: S
authors.lastname: Collins
authors.affiliation: Cancer Research Laboratory, Division of Science and Mathematics, University of the District of Columbia, Washington, DC, USA.|Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
authors.forename: Deepak
authors.initials: D
authors.lastname: Kumar
mesh_terms: D019540:Area Under Curve; D001343:Autophagy / Q000502:physiology*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D015153:Blotting, Western; D045744:Cell Line, Tumor; D016922:Cellular Senescence / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D061346:Laser Capture Microdissection; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology*; D034741:RNA, Small Interfering; D012372:ROC Curve; D056564:Sirtuin 1 / Q000378:metabolism*; D014162:Transfection
chemical_list: D014408:Biomarkers, Tumor; C551411:MIRN212 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: SIRT1; autophagy; miR-212; microRNA; prostate cancer