title: Preparation, characterisation and use for antioxidant oligosaccharides of a cellulase from abalone (Haliotis discus hannai) viscera.
abstract: BACKGROUND
  In China, abalone (Haliotis discus hannai) production is growing annually. During industrial processing, the viscera, which are abundant of cellulase, are usually discarded or processed into low-value feedstuff. Thus, it is of interest to obtain cellulase from abalone viscera and investigate its application for preparation of functional oligosaccharides.
  
  
  RESULTS
  A cellulase was purified from the hepatopancreas of abalone by ammonium sulfate precipitation and two-steps column chromatography. The molecular weight of the cellulase was 45 kDa on SDS-PAGE. Peptide mass fingerprinting analysis yielded 103 amino acid residues, which were identical to cellulases from other species of abalone. Substrate specificity analysis indicated that the cellulase is an endo-1,4-β-glucanase. Hydrolysis of seaweed Porphyra haitanensis polysaccharides by the enzyme produced oligosaccharides with degree of polymerisation of two to four, whose monosaccharide composition was 58% galactose, 4% glucose and 38% xylose. The oligosaccharides revealed 2,2'-diphenyl-1-picrylhydrazyl free radical as well as hydrogen peroxide scavenging activity.
  
  
  CONCLUSIONS
  It is feasible and meaningful to utilise cellulase from the viscera of abalone for preparation of functional oligosaccharides. © 2015 Society of Chemical Industry.
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
authors.forename: Zhi-Peng
authors.initials: ZP
authors.lastname: Tao
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, Fujian Province, 361102, China.
authors.forename: Le-Chang
authors.initials: LC
authors.lastname: Sun
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
authors.forename: Xu-Jian
authors.initials: XJ
authors.lastname: Qiu
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, Fujian Province, 361102, China.
authors.forename: Qiu-Feng
authors.initials: QF
authors.lastname: Cai
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, Fujian Province, 361102, China.
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Liu
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, Fujian Province, 361102, China.
authors.forename: Wen-Jin
authors.initials: WJ
authors.lastname: Su
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, Fujian Province, 361102, China.
authors.forename: Min-Jie
authors.initials: MJ
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification* / Q000378:metabolism; D017756:Aquaculture / Q000191:economics; D002240:Carbohydrate Sequence; D002681:China; D044644:Endo-1,3(4)-beta-Glucanase / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification* / Q000378:metabolism; D004795:Enzyme Stability; D005240:Feasibility Studies; D005520:Food Preservatives / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification / Q000378:metabolism; D049851:Gastropoda / Q000201:enzymology*; D043143:Hepatopancreas / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007220:Industrial Waste / Q000032:analysis / Q000191:economics; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification* / Q000378:metabolism; D044664:Porphyra / Q000737:chemistry*; D012624:Seaweed / Q000737:chemistry*; D013379:Substrate Specificity; D013696:Temperature; D014781:Viscera / Q000201:enzymology*
chemical_list: D000975:Antioxidants; D005520:Food Preservatives; D007220:Industrial Waste; D009844:Oligosaccharides; D044644:Endo-1,3(4)-beta-Glucanase
keywords: Porphyra haitanensis; abalone Haliotis discus hannai; antioxidant activity; cellulase; oligosaccharides; purification