title: Human microvascular endothelial cells displaying reduced angiogenesis and increased uptake of lipids during in vitro culture.
abstract: Human microvascular ECs from the neonatal foreskin of two donors purchased from one distributor were used in an angiogenesis assay under the same culture conditions. Different angiogenic potency was apparent in these two batches (ECang and ECnon-ang). During the cultivation period of three weeks, ECang ran through all stages of angiogenesis starting from proliferation to migration up to the formation of three-dimensional capillary-like structures. Despite of expression of endothelial markers, ECnon-ang showed excessive intracellular storage of lipids in form of multilamellar bodies and decreased angiogenic potency in contrast to its counterpart, ECang. Results indicate that lipid metabolism differs in ECang versus ECnon-ang. This study points up that these differences are based on the different donors and presents a novel and valuable model for the study of mechanisms of atherosclerosis in endothelial cells in vitro.
authors.affiliation: Institute of Veterinary Anatomy, Department of Veterinary Medicine, Freie Universität Berlin, Germany.
authors.forename: Henrieke
authors.initials: H
authors.lastname: Sievers
authors.affiliation: SFB 1112, Institute of Chemistry and Biochemistry - Physical and Theoretical Chemistry, Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin, Germany.
authors.forename: Ruth M
authors.initials: RM
authors.lastname: Hirschberg
authors.affiliation: Center for Medical Basic Research, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Hiebl
authors.affiliation: Institute of Veterinary Anatomy, Department of Veterinary Medicine, Freie Universität Berlin, Germany.
authors.forename: Hana
authors.initials: H
authors.lastname: Hünigen
authors.affiliation: Institute of Veterinary Anatomy, Department of Veterinary Medicine, Freie Universität Berlin, Germany.
authors.forename: Johanna
authors.initials: J
authors.lastname: Plendl
mesh_terms: D050197:Atherosclerosis / Q000503:physiopathology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism / Q000502:physiology*; D052816:Foreskin / Q000098:blood supply* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D050356:Lipid Metabolism; D008297:Male; D008853:Microscopy; D055806:Microvessels / Q000378:metabolism / Q000502:physiology*; D018919:Neovascularization, Physiologic / Q000502:physiology*
keywords: Foreskin; atherosclerosis; fetal bovine serum; multilamellar bodies