title: Human Fetal and Adult Chondrocytes.
abstract: As the only cell type found in healthy adult cartilage, chondrocytes are the obvious and most direct starting point for cartilage tissue engineering. Human adult, juvenile, neonatal, and fetal chondrocytes have all been demonstrated to produce cartilage matrix components in vitro for production of engineered tissues. In this chapter, procedures are outlined for isolation of chondrocytes from human fetal and adult cartilage. Methods for expansion and cryopreservation of the cells and characterization of gene expression using quantitative polymerase chain reaction (Q-PCR) analysis are also described.
authors.affiliation: Westmead Millennium Institute for Medical Research, University of Sydney, Westmead, NSW, 2145, Australia. kifah.shahin@health.nsw.gov.au.
authors.forename: Kifah
authors.initials: K
authors.lastname: Shahin
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
authors.forename: Nastaran
authors.initials: N
authors.lastname: Mahmoudifar
authors.affiliation: Faculty of Science, Engineering and Technology, Swinburne University of Technology, P.O. Box 218, Hawthorn, Melbourne, VIC, 3122, Australia.
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Doran
mesh_terms: D053687:Adult Stem Cells / Q000502:physiology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000502:physiology*; D020219:Chondrogenesis; D015925:Cryopreservation; D053686:Fetal Stem Cells / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D010641:Phenotype; D060888:Real-Time Polymerase Chain Reaction; D044968:Regenerative Medicine / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*
keywords: Chondrocyte; Collagenase; Cryopreservation; Dedifferentiation; Human adult and fetal tissue; Q-PCR