title: In-Depth Characterization of Sheep (Ovis aries) Milk Whey Proteome and Comparison with Cow (Bos taurus).
abstract: An in-depth proteomic study of sheep milk whey is reported and compared to the data available in the literature for the cow whey proteome. A combinatorial peptide ligand library kit (ProteoMiner) was used to normalize protein abundance in the sheep whey proteome followed by an in-gel digest of a 1D-PAGE display and an in-solution digestion followed by OFFGEL isoelectric focusing fractionation. The peptide fractions obtained were then analyzed by LC-MS/MS. This enabled identification of 669 proteins in sheep whey that, to our knowledge, is the largest inventory of sheep whey proteins identified to date. A comprehensive list of cow whey proteins currently available in the literature (783 proteins from unique genes) was assembled and compared to the sheep whey proteome data obtained in this study (606 proteins from unique genes). This comparison revealed that while the 233 proteins shared by the two species were significantly enriched for immune and inflammatory responses in gene ontology analysis, proteins only found in sheep whey in this study were identified that take part in both cellular development and immune responses, whereas proteins only found in cow whey in this study were identified to be associated with metabolism and cellular growth.
authors.affiliation: Department of Biochemistry, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand.
authors.forename: Minh
authors.initials: M
authors.lastname: Ha
authors.affiliation: Department of Oral Sciences, School of Dentistry, University of Otago, Dunedin, New Zealand.
authors.forename: Manya
authors.initials: M
authors.lastname: Sabherwal
authors.affiliation: Laboratory for Evolution and Development, Genetics Otago & Gravida; National Centre for Growth and Development, Department of Biochemistry, University of Otago, Dunedin, Aotearoa-New Zealand.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Duncan
authors.affiliation: Department of Biochemistry, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand.
authors.forename: Stewart
authors.initials: S
authors.lastname: Stevens
authors.affiliation: Department of Biochemistry, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand.
authors.forename: Peter
authors.initials: P
authors.lastname: Stockwell
authors.affiliation: Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand.
authors.forename: Michelle
authors.initials: M
authors.lastname: McConnell
authors.affiliation: Department of Food Science, University of Otago, Dunedin, New Zealand.
authors.forename: Alaa El-Din
authors.initials: Ael-D
authors.lastname: Bekhit
authors.affiliation: Department of Biochemistry, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand.
authors.forename: Alan
authors.initials: A
authors.lastname: Carne
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008892:Milk / Q000378:metabolism*; D010455:Peptides / Q000032:analysis; D020543:Proteome / Q000302:isolation & purification / Q000378:metabolism*; D040901:Proteomics; D012756:Sheep; D034561:Sheep, Domestic; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000378:metabolism; D000067816:Whey Proteins / Q000378:metabolism*
chemical_list: D010455:Peptides; D020543:Proteome; D000067816:Whey Proteins; D014357:Trypsin