title: Expression Levels of the Oxidative Stress Response Gene ALDH3A2 in Granulosa-Lutein Cells Are Related to Female Age and Infertility Diagnosis.
abstract: Oxidative stress (OS) plays an important role in all physiological processes. The effect of OS on cellular processes is modulated by the ability of the cell to express genes implicated in the reversal of lipid, protein, and DNA injury. Aldehyde dehydrogenase 3, member A2 (ALDH3A2) is a ubiquitous enzyme involved in lipid detoxification. The objective of this study was to investigate the expression ofALDH3A2in human granulosa-lutein (GL) cells of women undergoing in vitro fertilization (IVF) and its relationship with age, infertility diagnosis, and IVF outcome variables. Relative expression levels ofALDH3A2were determined by quantitative reverse transcription-polymerase chain reaction. To investigate the effect of age onALDH3A2expression, 72 women between 18 and 44 years of age with no ovarian factor (NOF) were analyzed. To evaluate the effect of infertility diagnosis onALDH3A2expression, the following groups were analyzed: 22 oocyte donors (ODs), 24 women >40 years old (yo) with tubal or male factor and no ovarian pathology, 18 poor responders (PRs), 19 cases with endometriosis (EM), and 18 patients with polycystic ovarian syndrome (PCOS). In NOF,ALDH3A2expression correlated positively with age and with the doses of follicle-stimulating hormone and luteinizing hormone administered and negatively with the number of total and mature oocytes. When different groups were analyzed,ALDH3A2expression levels were higher in patients >40 yo and in PR compared to OD. On the contrary, EM and PCOS levels were lower than expected for age. These data suggest that GL cellALDH3A2expression levels correlate with age, cause of infertility, and ovarian response to stimulation.
authors.affiliation: Laboratorio de Biología del Desarrollo, UD de Bioquímica y Biología Molecular, Universidad de la Laguna, La Laguna, Spain.
authors.forename: Rebeca
authors.initials: R
authors.lastname: González-Fernández
authors.affiliation: Centro de Asistencia a la Reproducción Humana de Canarias, La Laguna, Spain.
authors.forename: Jairo
authors.initials: J
authors.lastname: Hernández
authors.affiliation: Laboratorio de Biología del Desarrollo, UD de Bioquímica y Biología Molecular, Universidad de la Laguna, La Laguna, Spain CIBICAN, Universidad de La Laguna, La Laguna, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Martín-Vasallo
authors.affiliation: Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Puopolo
authors.affiliation: Centro de Asistencia a la Reproducción Humana de Canarias, La Laguna, Spain Department of Obstetrics and Gynecology, New York University, New York, NY, USA apalumbo@fivap.com.
authors.forename: Angela
authors.initials: A
authors.lastname: Palumbo
authors.affiliation: Laboratorio de Biología del Desarrollo, UD de Bioquímica y Biología Molecular, Universidad de la Laguna, La Laguna, Spain CIBICAN, Universidad de La Laguna, La Laguna, Spain.
authors.forename: Julio
authors.initials: J
authors.lastname: Ávila
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000445:Aldehyde Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000235:genetics / Q000378:metabolism* / Q000628:therapy; D008184:Luteal Cells / Q000378:metabolism*; D008297:Male; D010062:Ovulation Induction / Q000379:methods; D018384:Oxidative Stress / Q000502:physiology*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D000445:Aldehyde Oxidoreductases; C038828:long-chain-aldehyde dehydrogenase
keywords: ALDH3A2; PCOS; endometriosis; granulosa cells; oxidative stress