title: Peptide-Carrier Conjugation.
abstract: To produce antibodies against synthetic peptides it is necessary to couple them to a protein carrier. This chapter provides a nonspecialist overview of peptide-carrier conjugation. Furthermore, a protocol for coupling cysteine-containing peptides to bovine serum albumin is outlined.
authors.affiliation: Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark. prh@sund.ku.dk.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Hansen
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000276:immunology; D002417:Cattle; D003545:Cysteine / Q000737:chemistry; D010455:Peptides / Q000737:chemistry* / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology; D013388:Succinimides / Q000737:chemistry
chemical_list: D002352:Carrier Proteins; D010455:Peptides; D013388:Succinimides; C072252:m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester; D012710:Serum Albumin, Bovine; D003545:Cysteine
keywords: Bovine serum albumin; Peptide-carrier conjugation; m-Maleimidobenzoyl-N-hydroxysuccinimidyl ester (MBS)