title: Antibacterial effect of porcine PTX3 against Streptococcus suis type 2 infection.
abstract: Pentraxin 3 (PTX3), a soluble pattern recognition receptor, plays an important role in innate immunity and has been implicated to be a candidate resistance gene against Streptococcus suis 2 infection. To discover the antibacterial effect of porcine PTX3 against S. suis 2, the 42-kDa PTX3 protein was expressed by Chinese hamster ovary cells (CHO), and an additional eukaryotic expression vector pVAX-ptx3 was constructed. The expressed porcine PTX3 mediated a range of antibacterial activities including increasing phagocytic capacity of primary porcine alveolar macrophages (PAM) against S. suis 2 and inhibiting adhesion of S. suis 2 to human epidermoid cancer cells (Hep-2). In mouse model, pre-intramuscular injecting with pVAX-ptx3 reduced mortality and reduced bacteria loads in blood, spleen, lung and brain compared with that of control group during 2-12 h following intraperitoneal injection (i.p.) with S. suis 2. Meanwhile, the expressions of IL-6 and IL-8 in blood were increased in pVAX-ptx3 group, whereas no obvious changes about IL-10. In piglet model, bacteria load in blood of pVAX-ptx3 group was significantly lower than that of control group after i.p. with S. suis 2, correspondingly, expression of IL-6 and IL-8 were significantly increased in pVAX-ptx3 group. In contrast, white blood cell (WBC) and neutrophil cell (NEU) count of peripheral blood in pVAX-ptx3 group were lower than that of control group. These studies described a novel antibacterial role for porcine PTX3 against S. suis 2 both in vitro and in vivo and suggested that porcine PTX3 may be a potential biological agent against S. suis 2 in pig and be used for the clinical prevention and treatment of streptococcosis caused by S. suis 2.
authors.affiliation: OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: xjq870308@163.com.
authors.forename: Jueqiong
authors.initials: J
authors.lastname: Xu
authors.affiliation: OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 947212477@qq.com.
authors.forename: Yanjuan
authors.initials: Y
authors.lastname: Mu
authors.affiliation: OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2013107075@njau.edu.cn.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: wenyang.dong@mail.huji.ac.il.
authors.forename: Wenyang
authors.initials: W
authors.lastname: Dong
authors.affiliation: OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 408740856@qq.com.
authors.forename: Yinchu
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2013207019@njau.edu.cn.
authors.forename: Jiale
authors.initials: J
authors.lastname: Ma
authors.affiliation: OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 1164146939@qq.com.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Song
authors.affiliation: OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 1497873821@qq.com.
authors.forename: Zihao
authors.initials: Z
authors.lastname: Pan
authors.affiliation: OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 183448289@qq.com.
authors.forename: Chengping
authors.initials: C
authors.lastname: Lu
authors.affiliation: OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: yaohch@njau.edu.cn.
authors.forename: Huochun
authors.initials: H
authors.lastname: Yao
mesh_terms: D000825:Animal Structures / Q000382:microbiology; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D058491:Bacterial Load; D002097:C-Reactive Protein / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D004847:Epithelial Cells / Q000382:microbiology; D007155:Immunologic Factors / Q000008:administration & dosage / Q000494:pharmacology*; D007273:Injections, Intramuscular; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D051379:Mice; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D000683:Serum Amyloid P-Component / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D013290:Streptococcal Infections / Q000382:microbiology / Q000517:prevention & control; D017011:Streptococcus suis / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D016019:Survival Analysis; D013552:Swine; D000099153:Pentraxins
chemical_list: D002097:C-Reactive Protein; D007155:Immunologic Factors; D011994:Recombinant Proteins; D000683:Serum Amyloid P-Component; D000099153:Pentraxins
keywords: Antibacterial effect; Eukaryotic expression; Porcine PTX3 protein; S. suis 2