title: Analyzing the Effect of Aging on CD8+ T-Cell Phenotype Using Flow Cytometry.
abstract: One of the most noticeable changes in T-cell immunity with aging is the expansion of memory CD8+ T cells, with a decline in naïve phenotype T cells that reflects both diminished thymopoiesis and the effects of chronic antigenic stimulation with age. Flow cytometry is a useful tool in evaluating immune cells including the phenotype characteristics of different T-cell subsets. Here, we show flow cytometric methods measuring the different subsets of human CD8+ T cells that change with aging.
authors.affiliation: Section of Rheumatology, Department of Internal Medicine, Yale School of Medicine, 300 Cedar Street, New Haven, CT, 06520, USA.
authors.forename: Min Sun
authors.initials: MS
authors.lastname: Shin
authors.affiliation: Section of Rheumatology, Department of Internal Medicine, Yale School of Medicine, 300 Cedar Street, New Haven, CT, 06520, USA. Insoo.kang@yale.edu.
authors.forename: Insoo
authors.initials: I
authors.lastname: Kang
mesh_terms: D000375:Aging; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism
keywords: Flow cytometry; Human; T cells