title: A determinant of disease symptom severity is located in RNA2 of broad bean wilt virus 2.
abstract: Broad bean wilt virus 2 (BBWV2), which belongs to the genus Fabavirus, is a destructive pathogen of many economically important horticultural and ornamental crops. In this study, we constructed infectious full-length cDNA clones of two distinct isolates of BBWV2 under control of the cauliflower mosaic virus 35S promoter. BBWV2-PAP1 isolated from paprika (Capsicum annuum var. gulosum) induces severe disease symptoms in various pepper varieties, whereas BBWV2-RP1 isolated from red pepper (Capsicum annuum L.) causes mild symptoms. Agrobacterium-mediated inoculation of the infectious cDNA clones of BBWV2-PAP1 and RP1 resulted in the same symptoms as the original virus isolates. The infectious cDNA clones of BBWV2-PAP1 and RP1 were used to examine the symptoms induced by pseudorecombinants between the two isolates to localize in which of the two genomic RNAs are the symptom severity determinants in BBWV2. The pseudorecombinant of RP1-RNA1 and PAP1-RNA2 induced severe symptoms, similar to those caused by the parental isolate PAP1, whereas the pseudorecombinant of PAP1-RNA1 and RP1-RNA2 induced mild symptoms, similar to those caused by the parental isolate RP1. Our results suggest that BBWV2 RNA2 contains a symptom determinant(s) capable of enhancing symptom severity.
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Hae-Ryun
authors.initials: HR
authors.lastname: Kwak
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Ye-Ji
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Jaedeok
authors.initials: J
authors.lastname: Kim
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Mi-Kyeong
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Department of Plant Medicine, Andong National University, Andong 760-749, Republic of Korea.
authors.forename: Jeong-Soo
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea. Electronic address: hschoi@korea.kr.
authors.forename: Hong-Soo
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea. Electronic address: jakyseo@korea.kr.
authors.forename: Jang-Kyun
authors.initials: JK
authors.lastname: Seo
mesh_terms: D002212:Capsicum / Q000821:virology; D019181:Fabavirus / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D000073718:Pancreatitis-Associated Proteins; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana / Q000821:virology; D014774:Virulence
chemical_list: D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D012367:RNA, Viral
keywords: Fabavirus; Infectious cDNA clone; Secoviridae