title: Effects of Two Traditional Chinese Cooking Oils, Canola and Pork, on pH and Cholic Acid Content of Faeces and Colon Tumorigenesis in Kunming Mice.
abstract: Faecal pH and cholate are two important factors that can affect colon tumorigenesis, and can be modified by diet. In this study, the effects of two Chinese traditional cooking oils (pork oil and canola/rapeseed oil) on the pH and the cholic acid content in feces, in addition to colon tumorigenesis, were studied in mice. Kunming mice were randomized into various groups; negative control group (NCG), azoxymethane control group (ACG), pork oil group (POG), and canola oil Ggroup (COG). Mice in the ACG were fed a basic rodent chow; mice in POG and COG were given 10% cooking oil rodent chow with the respective oil type. All mice were given four weekly AOM (azoxymethane) i.p. injections (10 mg/kg). The pH and cholic acid of the feces were examined every two weeks. Colon tumors, aberrant crypt foci and organ weights were examined 32 weeks following the final AOM injection. The results showed that canola oil significantly decreased faecal pH in female mice (P<0.05), but had no influence on feces pH in male mice (P>0.05). Pork oil significantly increased the feces pH in both male and female mice (P<0.05). No significant change was found in feces cholic acid content when mice were fed 10% pork oil or canola oil compared with the ACG. Although Kunming mice were not susceptible to AOM-induced tumorigenesis in terms of colon tumor incidence, pork oil significantly increased the ACF number in male mice. Canola oil showed no influence on ACF in either male or female mice. Our results indicate that cooking oil effects faecal pH, but does not affect the faecal cholic acid content and thus AOM-induced colon neoplastic ACF is modified by dietary fat.
authors.affiliation: School of Public Health, Kunming Medical University, Yunnan Province, China E-mail : hexqcn@aliyun.com.
authors.forename: Xiao-Qiong
authors.initials: XQ
authors.lastname: He
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Duan
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.forename: Zhong-Yu
authors.initials: ZY
authors.lastname: Song
authors.forename: Simon Angelo
authors.initials: SA
authors.lastname: Cichello
mesh_terms: D000818:Animals; D001397:Azoxymethane; D063646:Carcinogenesis / Q000187:drug effects; D019826:Cholic Acid / Q000032:analysis*; D003106:Colon / Q000473:pathology*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology*; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D005243:Feces / Q000737:chemistry*; D005260:Female; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008297:Male; D051379:Mice; D009821:Oils / Q000494:pharmacology*; D009929:Organ Size / Q000187:drug effects; D000074262:Rapeseed Oil; D013552:Swine
chemical_list: D004042:Dietary Fats, Unsaturated; D005229:Fatty Acids, Monounsaturated; D009821:Oils; D000074262:Rapeseed Oil; D019826:Cholic Acid; D001397:Azoxymethane