title: Enhancement of Localized Surface Plasmon Resonance polymer based biosensor chips using well-defined glycopolymers for lectin detection.
abstract: Poly(methyl methacrylate) polymer based Localized Surface Plasmon Resonance biosensor chips were successfully fabricated using glycopolymer brushes carrying glucose moieties for the detection of concanavalin A. Poly(pentafluorostyrene), with pre-determined polymer chain lengths, were synthesized via a reversible addition-fragmentation chain transfer polymerization technique. The synthesized poly(pentafluorostyrene), was subsequently converted into glycopolymers via a para-fluoro-thiol "click" reaction and grafted onto the surface of sensor chips. The "glycocluster effect" induced by pendent carbohydrate moieties enabled a stronger affinity for concanavalin A binding, which resulted in a dramatic expansion of the sensors' response range. It was discovered that the longer polymer brushes did not guarantee additional enhancements for the sensor chips. Instead, they could lead to higher detection limits. In this study, the limit of detection for the sensor chips was discovered to be 1.3nmolL(-1) with a saturated response at 1054.2nmolL(-1). In addition to the superior performance, the capabilities of the reported sensor chips can be easily manipulated to detect a diverse range of analytes by "clicking" various sensing elements onto the polymer brushes.
authors.affiliation: Centre for Advanced Macromolecular Design, School of Chemical Engineering, University of New South Wales, Sydney, Australia.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Centre for Advanced Macromolecular Design, School of Chemical Engineering, University of New South Wales, Sydney, Australia.
authors.forename: Kok Hou
authors.initials: KH
authors.lastname: Wong
authors.affiliation: Centre for Advanced Macromolecular Design, School of Chemical Engineering, University of New South Wales, Sydney, Australia. Electronic address: a.granville@unsw.edu.au.
authors.forename: Anthony Michael
authors.initials: AM
authors.lastname: Granville
mesh_terms: D015374:Biosensing Techniques; D003208:Concanavalin A / Q000032:analysis*; D005947:Glucose / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D019904:Polymethyl Methacrylate / Q000138:chemical synthesis / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D003208:Concanavalin A; D019904:Polymethyl Methacrylate; D005947:Glucose
keywords: Biosensor chips; Glycocluster effect; Localized Surface Plasmon Resonance; Poly(pentafluorostyrene); Reversible addition–fragmentation chain transfer