title: Biofilm formation on the surface of polylactide during its biodegradation in different environments.
abstract: The research was aimed at determining the abundance and viability of biofilm formed on the surface of polylactide (PLA) during its biodegradation in different environments. It was also aimed at isolating biofilm forming bacteria, determining their hydrolytic activity and taxonomic status. The first step was to evaluate PLA biodegradability in lake water, compost and soil, using OxiTop Control. The next step was to assess the ability of isolated bacteria to form biofilm in the investigated environments and to evaluate the biofilm structure. The results indicate that PLA is sensitive to biodegradation in any environment, particularly in compost. During this process biofilm of high viability was observed on the surface of PLA. Based on the 16S rRNA gene sequence, the biofilm-forming bacteria were classified as the following species: Acidovorax sp. LW9, Chryseobacterium sp. LW2, Aeromonas veronii LW8, Arthrobacter aurescens LG2, Arthrobacter sp. LG12, A. aurescens LG9, Elizabethkingia meningoseptica LK3, A. aurescens LK9, A. aurescens and LK7. The results show that different bacterial species formed biofilm of different abundance and hydrolytic activitiy levels.
authors.affiliation: Faculty Biology and Environment Protection, Department of Environmental Microbiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, Toruń, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Walczak
authors.affiliation: Faculty Biology and Environment Protection, Department of Environmental Microbiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, Toruń, Poland. Electronic address: swiontek@umk.pl.
authors.forename: Maria
authors.initials: M
authors.lastname: Swiontek Brzezinska
authors.affiliation: Faculty of Chemistry, Nicolaus Copernicus University, 87-100 Toruń, Poland.
authors.forename: Alina
authors.initials: A
authors.lastname: Sionkowska
authors.affiliation: Faculty of Chemistry, Nicolaus Copernicus University, 87-100 Toruń, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Michalska
authors.affiliation: Department of Biochemistry, Warsaw University of Life Sciences, SGGW, Nowoursynowska 159, Warsaw 02-776, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Jankiewicz
authors.affiliation: Faculty Biology and Environment Protection, Department of Environmental Microbiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, Toruń, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Deja-Sikora
mesh_terms: D001673:Biodegradation, Environmental; D018441:Biofilms; D017753:Ecosystem; D064307:Microbiota; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D011091:Polyesters; C033616:poly(lactide)
keywords: Biodegradation; Biofilm; Polylactide