title: The Role of the Y Box Binding Protein 1 C-Terminal Domain in Vascular Endothelial Cell Proliferation, Apoptosis, and Angiogenesis.
abstract: Different domains of the multifunctional transcription factor Y-box binding protein 1 (YB1) regulate proliferation, differentiation, and apoptosis by transactivating or repressing the promoters of various genes. Here we report that the C-terminal domain of YB1 (YB1 CTD) is involved in endothelial cell proliferation, apoptosis, and tube formation. The oligo pull-down assays demonstrated that YB1 directly binds double-stranded GC box sequences in endothelial cells through the 125-220 amino acids. Adenovirus expression vectors harboring green fluorescent protein (GFP) or GFP-tagged YB1 CTD were constructed and used to infect EA.hy926 endothelial cells. Overexpression of the YB1 CTD significantly increased p21 expression, decreased cyclin B1 expression, and inhibited the proliferation of EA.hy926 cells. YB1 CTD overexpression also increased Bax and active caspase 3 expression, decreased Bcl-2 expression, and induced apoptosis in EA.hy926 cells. Furthermore, overexpression of the YB1 CTD significantly suppressed migration and tube formation in EA.hy926 cells. Finally, YB1 CTD decreased ERK1/2 phosphorylation in EA.hy926 cells. These findings demonstrated vital roles for YB1 in endothelial cell proliferation, apoptosis, and tube formation through transcriptional regulation of GC box-related genes.
authors.affiliation: 1 Central Laboratory, Affiliated Hospital of Hebei University , Baoding, China .|2 Department of Cardiovascular Internal Medicine, Baoding First Central Hospital , Baoding, China .
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: 1 Central Laboratory, Affiliated Hospital of Hebei University , Baoding, China .
authors.forename: Hong-jie
authors.initials: HJ
authors.lastname: Wang
authors.affiliation: 1 Central Laboratory, Affiliated Hospital of Hebei University , Baoding, China .
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: 3 Department of Geriatrics, Baoding Second Hospital , Baoding, China .
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: 1 Central Laboratory, Affiliated Hospital of Hebei University , Baoding, China .
authors.forename: Yan
authors.initials: Y
authors.lastname: Qin
authors.affiliation: 1 Central Laboratory, Affiliated Hospital of Hebei University , Baoding, China .
authors.forename: Li-shuang
authors.initials: LS
authors.lastname: Zheng
authors.affiliation: 4 Department of Preventive Medicine, Hebei University , Baoding, China .
authors.forename: Jin-sa
authors.initials: JS
authors.lastname: Zhou
authors.affiliation: 4 Department of Preventive Medicine, Hebei University , Baoding, China .
authors.forename: Peng-huan
authors.initials: PH
authors.lastname: Qu
authors.affiliation: 1 Central Laboratory, Affiliated Hospital of Hebei University , Baoding, China .
authors.forename: Jian-hong
authors.initials: JH
authors.lastname: Shi
authors.affiliation: 5 Department of Kidney Internal Medicine, Affiliated Hospital of Hebei University , Baoding, China .
authors.forename: Hai-song
authors.initials: HS
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D018919:Neovascularization, Physiologic / Q000235:genetics*; D054730:Protein Interaction Domains and Motifs / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D014162:Transfection; D051840:Y-Box-Binding Protein 1 / Q000737:chemistry / Q000235:genetics / Q000502:physiology*
chemical_list: D015534:Trans-Activators; D051840:Y-Box-Binding Protein 1; D048049:Extracellular Signal-Regulated MAP Kinases