title: Design and preparation of quantum dots fluorescent probes for in situ identification of Microthrix parvicella in bulking sludge.
abstract: A series of quantum dots (QDs) fluorescent probes for the in situ identification of Microthrix parvicella (M. parvicella) in bulking sludge were designed and prepared. In the preparation of CdTe/CdS QDs, the 11-mercaptoundecanoic acid (11-acid) and 16-mercaptohexadecanoic acid (16-acid) were used as the stabilizer. The prepared QDs probes were characterized by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and transmission electron microscopy (TEM), and the results showed that the CdTe/CdS QDs formed a core-shell structure and the long carbon chain was successfully grafted onto its surface. And the three QDs probes had different crystallinity and particle size, which was due to the inhibition effect of long carbon chain. The optical properties test results showed that although the formed core-shell structure and long carbon chain affected the fluorescent intensity, adsorption, and emission spectra of the QDs probes, the probes B and C had a large stokes-shift of 82 and 101 nm, which was a benefit for their fluorescent labeling property. In the fluorescent identification of M. parvicella, the probes B and C effectively adsorbed onto the surface of M. parvicella through a hydrophobic bond, and then identified M. parvicella by their unique fluorescence. In addition, it was found that a better hydrophobic property resulted in better identification efficiency.
authors.affiliation: School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin, 300384, China. xueningfei@126.com.|School of Science, Tianjin Chengjian University, No. 26 Jinjing Road, Xiqing District, Tianjin, 300384, China. xueningfei@126.com.|School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China. xueningfei@126.com.
authors.forename: Xuening
authors.initials: X
authors.lastname: Fei
authors.affiliation: School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin, 300384, China.
authors.forename: Wenke
authors.initials: W
authors.lastname: Sun
authors.affiliation: School of Science, Tianjin Chengjian University, No. 26 Jinjing Road, Xiqing District, Tianjin, 300384, China. caolinyun54@126.com.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Cao
authors.affiliation: School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Jiao
authors.affiliation: School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Lin
authors.affiliation: School of Science, Tianjin Chengjian University, No. 26 Jinjing Road, Xiqing District, Tianjin, 300384, China.
authors.forename: Guozhi
authors.initials: G
authors.lastname: Jia
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000302:isolation & purification*; D003460:Crystallization; D005227:Fatty Acids; D005456:Fluorescent Dyes; D057927:Hydrophobic and Hydrophilic Interactions; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D010169:Palmitic Acids; D010316:Particle Size; D045663:Quantum Dots; D012722:Sewage / Q000382:microbiology*; D017550:Spectroscopy, Fourier Transform Infrared; D013438:Sulfhydryl Compounds; D013691:Tellurium; D014961:X-Ray Diffraction
chemical_list: C505222:11-mercaptoundecanoic acid; C450319:16-mercaptohexadecanoic acid; D005227:Fatty Acids; D005456:Fluorescent Dyes; D010169:Palmitic Acids; D012722:Sewage; D013438:Sulfhydryl Compounds; D013691:Tellurium
keywords: Fluorescent QDs probes; Hydrophobic; In situ identification; Microthrix parvicella