title: Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis.
abstract: Hydrogen gas (H2) was recently proposed as a therapeutic antioxidant and signaling molecule in clinical trials. However, the underlying physiological roles of H2 in plants remain unclear. In the present study, hydrogen-rich water (HRW) was used to characterize the physiological roles of H2 in enhancing the tolerance of Brassica campestris against cadmium (Cd). The results showed that both 50 μM CdCl2 and 50%-saturated HRW induced an increase of endogenous H2 in Brassica campestris seedlings, and HRW alleviated Cd toxicity related to growth inhibition and oxidative damage. Seedlings supplied with HRW exhibited increased root length and reduced lipid peroxidation, similar to plants receiving GSH post-treatment. Additionally, seedlings post-treated with HRW accumulated higher levels of reduced glutathione (GSH) and ascorbic acid (AsA) and showed increased GST and GPX activities in roots. Molecular evidence illustrated that the expression of genes such as GS, GR1 and GR2, which were down-regulated following the addition of Cd, GSH or BSO, could be reversed to varying degrees by the addition of HRW. Based on these results, it could be proposed that H2 might be an important regulator for enhancing the tolerance of Brassica campestris seedlings against Cd, mainly by governing reduced glutathione homeostasis.
authors.affiliation: College of Life Sciences, Laboratory of Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Laboratory of Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
authors.forename: Nana
authors.initials: N
authors.lastname: Su
authors.affiliation: College of Life Sciences, Laboratory of Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Laboratory of Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
authors.forename: Wenbiao
authors.initials: W
authors.lastname: Shen
authors.affiliation: College of Life Sciences, Laboratory of Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Shen
authors.affiliation: College of Life Sciences, Laboratory of Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xia
authors.affiliation: College of Life Sciences, Laboratory of Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Cui
mesh_terms: D001205:Ascorbic Acid / Q000378:metabolism; D001937:Brassica / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002104:Cadmium / Q000633:toxicity*; D004361:Drug Tolerance; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D005981:Glutathione Synthase / Q000235:genetics / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress / Q000187:drug effects*; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D060888:Real-Time Polymerase Chain Reaction; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism
chemical_list: D002104:Cadmium; D006859:Hydrogen; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D005981:Glutathione Synthase; D005978:Glutathione; D001205:Ascorbic Acid