title: Joint Transcriptomic and Metabolomic Analyses Reveal Changes in the Primary Metabolism and Imbalances in the Subgenome Orchestration in the Bread Wheat Molecular Response to Fusarium graminearum.
abstract: Fusarium head blight is a prevalent disease of bread wheat (Triticum aestivum L.), which leads to considerable losses in yield and quality. Quantitative resistance to the causative fungus Fusarium graminearum is poorly understood. We integrated transcriptomics and metabolomics data to dissect the molecular response to the fungus and its main virulence factor, the toxin deoxynivalenol in near-isogenic lines segregating for two resistance quantitative trait loci, Fhb1 and Qfhs.ifa-5A. The data sets portrait rearrangements in the primary metabolism and the translational machinery to counter the fungus and the effects of the toxin and highlight distinct changes in the metabolism of glutamate in lines carrying Qfhs.ifa-5A. These observations are possibly due to the activity of two amino acid permeases located in the quantitative trait locus confidence interval, which may contribute to increased pathogen endurance. Mapping to the highly resolved region of Fhb1 reduced the list of candidates to few genes that are specifically expressed in presence of the quantitative trait loci and in response to the pathogen, which include a receptor-like protein kinase, a protein kinase, and an E3 ubiquitin-protein ligase. On a genome-scale level, the individual subgenomes of hexaploid wheat contribute differentially to defense. In particular, the D subgenome exhibited a pronounced response to the pathogen and contributed significantly to the overall defense response.
authors.affiliation: Plant Genome and Systems Biology, Helmholtz Zentrum München, Neuherberg, D-85764, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Nussbaumer
authors.affiliation: Center for Analytical Chemistry (IFA-Tulln), BOKU - University of Natural Resources and Life Sciences, Tulln, A- 3430, Austria.
authors.forename: Benedikt
authors.initials: B
authors.lastname: Warth
authors.affiliation: Plant Genome and Systems Biology, Helmholtz Zentrum München, Neuherberg, D-85764, Germany.
authors.forename: Sapna
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Institute for Biotechnology in Plant Production (IFA-Tulln), BOKU - University of Natural Resources and Life Sciences, Tulln, A-3430, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Ametz
authors.affiliation: Center for Analytical Chemistry (IFA-Tulln), BOKU - University of Natural Resources and Life Sciences, Tulln, A- 3430, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Bueschl
authors.affiliation: Center for Analytical Chemistry (IFA-Tulln), BOKU - University of Natural Resources and Life Sciences, Tulln, A- 3430, Austria.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Parich
authors.affiliation: Plant Genome and Systems Biology, Helmholtz Zentrum München, Neuherberg, D-85764, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Pfeifer
authors.affiliation: Institute for Biotechnology in Plant Production (IFA-Tulln), BOKU - University of Natural Resources and Life Sciences, Tulln, A-3430, Austria.
authors.forename: Gerald
authors.initials: G
authors.lastname: Siegwart
authors.affiliation: Institute for Biotechnology in Plant Production (IFA-Tulln), BOKU - University of Natural Resources and Life Sciences, Tulln, A-3430, Austria.
authors.forename: Barbara
authors.initials: B
authors.lastname: Steiner
authors.affiliation: Institute for Biotechnology in Plant Production (IFA-Tulln), BOKU - University of Natural Resources and Life Sciences, Tulln, A-3430, Austria.
authors.forename: Marc
authors.initials: M
authors.lastname: Lemmens
authors.affiliation: Center for Analytical Chemistry (IFA-Tulln), BOKU - University of Natural Resources and Life Sciences, Tulln, A- 3430, Austria.
authors.forename: Rainer
authors.initials: R
authors.lastname: Schuhmacher
authors.affiliation: Institute for Biotechnology in Plant Production (IFA-Tulln), BOKU - University of Natural Resources and Life Sciences, Tulln, A-3430, Austria.
authors.forename: Hermann
authors.initials: H
authors.lastname: Buerstmayr
authors.affiliation: Plant Genome and Systems Biology, Helmholtz Zentrum München, Neuherberg, D-85764, Germany.
authors.forename: Klaus F X
authors.initials: KF
authors.lastname: Mayer
authors.affiliation: Plant Genome and Systems Biology, Helmholtz Zentrum München, Neuherberg, D-85764, Germany karl.kugler@helmholtz-muenchen.de wolfgang.schweiger@boku.ac.at.
authors.forename: Karl G
authors.initials: KG
authors.lastname: Kugler
authors.affiliation: Institute for Biotechnology in Plant Production (IFA-Tulln), BOKU - University of Natural Resources and Life Sciences, Tulln, A-3430, Austria karl.kugler@helmholtz-muenchen.de wolfgang.schweiger@boku.ac.at.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schweiger
mesh_terms: D001481:Basal Metabolism; D019295:Computational Biology / Q000379:methods; D060467:Disease Resistance / Q000235:genetics; D005670:Fusarium / Q000502:physiology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D023281:Genomics / Q000379:methods; D018698:Glutamic Acid; D054884:Host-Pathogen Interactions / Q000235:genetics; D053858:Metabolic Networks and Pathways; D055442:Metabolome; D055432:Metabolomics; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D040641:Quantitative Trait Loci; D011130:RNA Ligase (ATP) / Q000378:metabolism; D059467:Transcriptome; D014255:Trichothecenes / Q000633:toxicity; D014908:Triticum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000382:microbiology; D054875:Ubiquitination
chemical_list: D014255:Trichothecenes; D018698:Glutamic Acid; D011130:RNA Ligase (ATP); C007262:deoxynivalenol
keywords: Fhb1; Fusarium head blight; Qfhs.ifa-5A; deoxynivalenol; resistance QTL