title: Complete genome sequence of the siphoviral bacteriophage Βϕ-R3177, which lyses an OXA-66-producing carbapenem-resistant Acinetobacter baumannii isolate.
abstract: In recent years, antimicrobial resistance has become a major medical threat worldwide. Among these threats, the rapid increase in carbapenem-resistant Acinetobacter baumannii (CRAB) is a particularly challenging global issue in the health care setting. In this study, a novel lytic A. baumannii phage, Βϕ-R3177, infecting carbapenem-resistant A. baumannii strains was isolated from sewage samples at a hospital. The morphology of the phage as assessed by transmission electron microscopy (TEM) indicated that it belongs to the family Siphoviridae within the order Caudovirales. It has a linear double-stranded DNA genome of 47,575 bp with a G+C content of 39.83%. Eighty open reading frames (ORFs) were predicted; however, only 14 ORFs were annotated as encoding functional proteins, while most of the ORFs encoded hypothetical proteins. Among the total ORFs of the phage genome, no toxin-related genes were detected. A bioinformatics analysis showed that the whole genome sequence of phage Βϕ-R3177 exhibited 62% sequence similarity to that of Acinetobacter phage Βϕ-B1252, but there was no homology seen with other phages. Physiological characteristics, such as one-step growth properties, pH and temperature stability, and host cell lysis activity showed this phage has high stability and lytic activity against host bacteria and therefore has potential applicability as an antibacterial agent to control pathogens in the hospital environment.
authors.affiliation: Department of Laboratory Medicine and Research Institute of Antimicrobial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Republic of Korea.
authors.forename: Jongsoo
authors.initials: J
authors.lastname: Jeon
authors.affiliation: Department of Laboratory Medicine and Research Institute of Antimicrobial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Republic of Korea.
authors.forename: Roshan
authors.initials: R
authors.lastname: D'Souza
authors.affiliation: Department of Laboratory Medicine and Research Institute of Antimicrobial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Republic of Korea.
authors.forename: Naina
authors.initials: N
authors.lastname: Pinto
authors.affiliation: Molecular Phytobacteriology Laboratory, KRIBB, Daejeon, South Korea.|Biosystems and Bioengineering Program, School of Science, University of Science and Technology (UST), Daejeon, Republic of Korea.
authors.forename: Choong-Min
authors.initials: CM
authors.lastname: Ryu
authors.affiliation: Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National University, Gwang-ju, 500-757, Republic of Korea.
authors.forename: Jong-hwan
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine and Research Institute of Antimicrobial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Republic of Korea. deyong@yuhs.ac.
authors.forename: Dongeun
authors.initials: D
authors.lastname: Yong
authors.affiliation: Department of Laboratory Medicine and Research Institute of Antimicrobial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Republic of Korea.
authors.forename: Kyungwon
authors.initials: K
authors.lastname: Lee
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology / Q000821:virology*; D001426:Bacterial Proteins / Q000378:metabolism; D001435:Bacteriophages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D001483:Base Sequence; D015780:Carbapenems / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017906:Siphoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases