title: ER stress-inducible protein MANF selectively expresses in human spleen.
abstract: Mesencephalic astrocyte-derived neurotrophic factor (MANF; also known as arginine-rich, mutated in early tumors; ARMET), is an ER stress-inducible protein, and widely expressed in mammalian tissues. In this study, we are interested in the profile of MANF expression in human splenocytes. Three patients with spleen trauma were enrolled in this study. Immunohistochemistry and immunofluorescence were used to detect MANF expression in the four types of cells, including T cells, B cells, plasma cells, and macrophages in spleens by using the specific antibodies of anti-CD3, anti-CD20, anti-CD138, and anti-CD68, respectively. We found that MANF-positive cells extensively distributed in the red pulp and marginal-zone of spleen, and MANF was almost localized in the cytoplasm of splenocytes. Double immunofluorescent staining results showed that MANF localized mainly in the plasma cells and macrophages, but not in T and B cells. Meanwhile, we found that some MANF-positive cells expressed ER stress-related proteins, including ATF6, XBP1s, BiP, and CHOP. These results suggest that the selective expression of MANF in splenocytes may be involved in plasma cell differentiation and immune regulation.
authors.affiliation: School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; School of Pharmacy, Anhui Medical University, Hefei 230032, China; Biopharmaceutical Research Institute, Anhui Medical University, Hefei 230032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: The 4th Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
authors.forename: Chengyue
authors.initials: C
authors.lastname: Zhou
authors.affiliation: School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; Biopharmaceutical Research Institute, Anhui Medical University, Hefei 230032, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Tao
authors.affiliation: School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; Biopharmaceutical Research Institute, Anhui Medical University, Hefei 230032, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Feng
authors.affiliation: School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; Biopharmaceutical Research Institute, Anhui Medical University, Hefei 230032, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
authors.forename: Lijian
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; Biopharmaceutical Research Institute, Anhui Medical University, Hefei 230032, China.
authors.forename: Chengjin
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China.
authors.forename: Dake
authors.initials: D
authors.lastname: Huang
authors.affiliation: School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; Biopharmaceutical Research Institute, Anhui Medical University, Hefei 230032, China.
authors.forename: Shengyun
authors.initials: S
authors.lastname: Fang
authors.affiliation: School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; Biopharmaceutical Research Institute, Anhui Medical University, Hefei 230032, China. Electronic address: shenyx@ahmu.edu.cn.
authors.forename: Yuxian
authors.initials: Y
authors.lastname: Shen
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D010950:Plasma Cells / Q000276:immunology / Q000378:metabolism; D021381:Protein Transport; D013154:Spleen / Q000276:immunology* / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
chemical_list: C099695:MANF protein, human; D009414:Nerve Growth Factors
keywords: Endoplasmic reticulum stress; MANF; Plasma cells; Spleen; Unfolded protein response