title: Efficient Gene Editing in Primary Human T Cells.
abstract: Recent advances in T-cell therapy for cancer, viral infections, and autoimmune diseases highlight the broad therapeutic potential of T-cell engineering. However, site-specific genetic manipulation in primary human T cells remains challenging. Two recent studies describe efficient genome editing in T cells using CRISPR and TALEN approaches.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California-Los Angeles, Los Angeles, CA 90095, USA. Electronic address: yvchen@ucla.edu.
authors.forename: Yvonne Y
authors.initials: YY
authors.lastname: Chen
mesh_terms: D001327:Autoimmune Diseases / Q000276:immunology / Q000628:therapy; D064113:CRISPR-Cas Systems / Q000235:genetics*; D002478:Cells, Cultured; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism*; D005818:Genetic Engineering; D006801:Humans; D007167:Immunotherapy; D009369:Neoplasms / Q000276:immunology / Q000628:therapy; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D014777:Virus Diseases / Q000276:immunology / Q000628:therapy
chemical_list: D004720:Endonucleases