title: The Y3** ncRNA promotes the 3' end processing of histone mRNAs.
abstract: We demonstrate that the Y3/Y3** noncoding RNAs (ncRNAs) bind to the CPSF (cleavage and polyadenylation specificity factor) and that Y3** associates with the 3' untranslated region (UTR) of histone pre-mRNAs. The depletion of Y3** impairs the 3' end processing of histone pre-mRNAs as well as the formation and protein dynamics of histone locus bodies (HLBs), the site of histone mRNA synthesis and processing. HLB morphology is also disturbed by knockdown of the CPSF but not the U7-snRNP components. In conclusion, we propose that the Y3** ncRNA promotes the 3' end processing of histone pre-mRNAs by enhancing the recruitment of the CPSF to histone pre-mRNAs at HLBs.
authors.affiliation: Institute of Molecular Medicine, Martin-Luther-University Halle-Wittenberg, Saxony-Anhalt 06120, Germany;
authors.forename: Marcel
authors.initials: M
authors.lastname: Köhn
authors.affiliation: Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Saxony-Anhalt 06120, Germany;
authors.forename: Christian
authors.initials: C
authors.lastname: Ihling
authors.affiliation: Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Saxony-Anhalt 06120, Germany;
authors.forename: Andrea
authors.initials: A
authors.lastname: Sinz
authors.affiliation: Interdisziplinäres Zentrum für Klinische Forschung, Core Unit DNA-Technologies, University Leipzig, Saxony 04103, Germany.
authors.forename: Knut
authors.initials: K
authors.lastname: Krohn
authors.affiliation: Institute of Molecular Medicine, Martin-Luther-University Halle-Wittenberg, Saxony-Anhalt 06120, Germany;
authors.forename: Stefan
authors.initials: S
authors.lastname: Hüttelmaier
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006657:Histones / Q000235:genetics*; D006801:Humans; D026721:RNA 3' End Processing / Q000235:genetics*; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D006657:Histones; D012322:RNA Precursors; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; C000604050:Y3 non-coding RNA, human
keywords: CPSF; Y RNA; Y3**; histone locus body; histone mRNA processing