title: Antidiabetic effect of Ficus racemosa Linn. stem bark in high-fat diet and low-dose streptozotocin-induced type 2 diabetic rats: a mechanistic study.
abstract: The present study was designed to investigate the effects of the ethanol extract of Ficus racemosa (FRE) on biochemical parameters in type 2-like diabetes, induced by a combination of standardised high-fat diet and low-dose streptozotocin (25mgkg(-1), i.p.) in rats. To elucidate the mode of action of FRE, its effects on a battery of targets involved in glucose homeostasis was evaluated. FRE (200 and 400mgkg(-1), p.o.), in a dose-dependent manner, altered the biochemical parameters and significantly improved glucose tolerance and HDL-c levels. In different bioassays, FRE showed inhibition of PTP-1B (IC50 12.1μg/mL) and DPP-IV (42.5%). FRE exhibited 82.6% binding to PPAR-γ. Furthermore FRE exhibited stimulation of glucose uptake by skeletal muscles (hemi-diaphragm). Bergenin was quantified in bioactive-FRE by high-performance liquid chromatography (0.15%w/w). This is the first report demonstrating the effectiveness of F. racemosa stem bark in type 2 diabetes and targets involved in it.
authors.affiliation: Manipal College of Pharmaceutical Sciences, Manipal 576 104, Karnataka, India; Sree Siddaganga College of Pharmacy, Tumkur 572 102, Karnataka, India. Electronic address: veeresh36@gmail.com.
authors.forename: V P
authors.initials: VP
authors.lastname: Veerapur
authors.affiliation: Manipal College of Pharmaceutical Sciences, Manipal 576 104, Karnataka, India.
authors.forename: K R
authors.initials: KR
authors.lastname: Prabhakar
authors.affiliation: Sree Siddaganga College of Pharmacy, Tumkur 572 102, Karnataka, India.
authors.forename: B S
authors.initials: BS
authors.lastname: Thippeswamy
authors.affiliation: Manipal College of Pharmaceutical Sciences, Manipal 576 104, Karnataka, India.
authors.forename: Punit
authors.initials: P
authors.lastname: Bansal
authors.affiliation: Manipal College of Pharmaceutical Sciences, Manipal 576 104, Karnataka, India.
authors.forename: K K
authors.initials: KK
authors.lastname: Srinivasan
authors.affiliation: Manipal College of Pharmaceutical Sciences, Manipal 576 104, Karnataka, India.
authors.forename: M K
authors.initials: MK
authors.lastname: Unnikrishnan
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy*; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D030681:Ficus / Q000737:chemistry*; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D008297:Male; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D051381:Rats; D011920:Rats, Inbred WF; D017208:Rats, Wistar; D013311:Streptozocin / Q000009:adverse effects*
chemical_list: D007004:Hypoglycemic Agents; D010936:Plant Extracts; D013311:Streptozocin
keywords: Bergenin; DPP-IV; Ficus racemosa; Glucose uptake; High-fat diet-fed; PPAR-γ; PTB-1B; STZ