title: Simultaneous qualitation and quantitation of natural trans-1,4-polyisoprene from Eucommia ulmoides Oliver by gel permeation chromatography (GPC).
abstract: Natural trans-1,4-polyisoprene (TPI) as a functional biomaterial has aroused great interest for rubber industrial product use. Here, we proposed a method that enables simultaneous analysis of the content and molecular-weight distribution (MWD) of natural TPI by gel permeation chromatography (GPC). The natural TPIs were collected from leaves, fruit coatings and bark of Eucommia ulmoides Oliver (E. ulmoides) through toluene extraction followed by ethanol purification. The results of TPI contents from leaves and fruit coatings were shown ca. 3.5% and 13.8%, respectively. Accordingly, limits of detection (LODs) of TPI were 0.58mg/mL from leaves and 0.47mg/mL from fruit coatings. The MWDs of TPI demonstrated a bimodal distribution from leaves, a unimodal distribution from bark, and a unimodal distribution with a tiny peak shoulder from fruit coatings. In real-life E. ulmoides analysis, the results from three independent methods (GPC, gravimetric method, and infrared spectroscopy) were obtained with good consistency.
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Tianyang
authors.initials: T
authors.lastname: Guo
authors.affiliation: Center of Advanced Elastomer Materials, College of Materials and Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Yuanbo
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Non-timber Forest Research and Development Center, Chinese Academy of Forestry, and China Paulownia Research and Development Center, Zhengzhou 450003, PR China.
authors.forename: Yanxiu
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ma
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Qiuming
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ni
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Zequn
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Sai
authors.initials: S
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: yydong@mail.buct.edu.cn.
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Center of Advanced Elastomer Materials, College of Materials and Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: duzhong@iccas.ac.cn.
authors.forename: Jichuan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Center of Advanced Elastomer Materials, College of Materials and Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Haijia
authors.initials: H
authors.lastname: Su
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
mesh_terms: D002850:Chromatography, Gel / Q000379:methods*; D031283:Eucommiaceae / Q000737:chemistry*; D045782:Hemiterpenes / Q000032:analysis* / Q000737:chemistry*; D007840:Latex / Q000032:analysis* / Q000737:chemistry*; D057230:Limit of Detection; D008970:Molecular Weight; D024301:Plant Bark / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D045782:Hemiterpenes; D007840:Latex; C455100:1,4-polyisoprene
keywords: Eucommia ulmoides oliver; Gel permeation chromatography (GPC); Molecular-weight distribution (MWD); Quantitation; trans-Polyisoprene (TPI)