title: Investigation on the effect of static magnetic field up to 30 mT on viability percent, proliferation rate and IC50 of HeLa and fibroblast cells.
abstract: We have investigated the effects of static magnetic field (SMF) on the viability of the human cervical cancer (HeLa) cell line and fibroblast cells. The cells were cultured in DMEM medium and treated several times (24, 48,72 and 96 h) and at several intensities (5, 10, 20 and 30 mT) of magnetic field (MF). The cytotoxicity and cell viability percent in treated cells were performed using MTT assay by evaluating mitochondrial dehydrogenase activity. The MF ability on inducing cell death or inhibiting biochemical function was reported as cell death percent. The results showed that the increase of MF intensity and the time that cells were exposed to this treatment increased sharply cell death percent and proliferation rate in HeLa cell compare to fibroblast cells. Our data suggest that SMF biological effects on cell death were different in our selected targets. Cell type and time of exposure have been therefore found to be significant factors. These findings could be used to improve new effective method using SMF in conjunction with the common therapeutic approaches.
authors.affiliation: a Department of Pharmacology and Toxicology , Faculty of Pharmacology, Ahvaz Jundishapur University of Medical Sciences , Ahvaz , Iran and.
authors.forename: Jaber
authors.initials: J
authors.lastname: Zafari
authors.affiliation: b Department of Biophysics , Faculty of Biological Sciences, Tarbiat Modares University (TMU) , Tehran , Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Javani Jouni
authors.affiliation: b Department of Biophysics , Faculty of Biological Sciences, Tarbiat Modares University (TMU) , Tehran , Iran.
authors.forename: Parviz
authors.initials: P
authors.lastname: Abdolmaleki
authors.affiliation: a Department of Pharmacology and Toxicology , Faculty of Pharmacology, Ahvaz Jundishapur University of Medical Sciences , Ahvaz , Iran and.
authors.forename: Amir
authors.initials: A
authors.lastname: Jalali
authors.affiliation: a Department of Pharmacology and Toxicology , Faculty of Pharmacology, Ahvaz Jundishapur University of Medical Sciences , Ahvaz , Iran and.
authors.forename: Mohammad Javad
authors.initials: MJ
authors.lastname: Khodayar
mesh_terms: D049109:Cell Proliferation; D002470:Cell Survival; D005347:Fibroblasts / Q000166:cytology*; D006367:HeLa Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D060526:Magnetic Fields / Q000009:adverse effects*
keywords: Fibroblast cells; human cervical cancer (HeLa) cell line; proliferation rate; static magnetic field; viability percent