title: Pelistega suis sp. nov., isolated from domestic and wild animals.
abstract: Biochemical and molecular genetic studies were performed on three novel Gram-stain-negative, catalase- and oxidase-positive, bacilli-shaped organisms isolated from the tonsils of two pigs and one wild boar. The micro-organism was identified as a species of the genus Pelistega based on its cellular morphological and biochemical tests. The closest phylogenetic relative of the novel bacilli was Pelistega indica HM-7T (98.2 % 16S rRNA gene sequence similarity to the type strain). groEL and gyrB sequence analysis showed interspecies divergence from the closest 16S rRNA gene phylogenetic relative, P. indica of 87.0.% and 69 %, respectively. The polyamine pattern contains predominantly putrescine and 2-hydroxyputrescine. The major quinone is ubiquinone Q-8 and in the polar lipid profile, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid and an unidentified lipid are predominant. The novel bacterial isolate can be distinguished from P. indica by several biochemical characteristics, such as the production of l-pyrrolydonil arylamidase but not gamma-glutamyl-transferase, and the utilization of different carbon sources. Based on both phenotypic and phylogenetic findings, the novel bacterium is classified as representing a novel species of the genus Pelistega, for which the name Pelistega suis sp. nov. is proposed. The type strain is 3340-03T ( = CECT 8400T = CCUG 64465T).
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense, 28040 Madrid, Spain.|Departamento de Sanidad Animal. Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Vela
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Perez Sancho
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Lucas
authors.initials: L
authors.lastname: Domínguez
authors.affiliation: Institut für Bakteriologie, Mykologie & Hygiene, Veterinärmedizinische Universität Wien, A-1210 Wien, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
authors.affiliation: Departamento de Sanidad Animal. Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.|Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Jose F
authors.initials: JF
authors.lastname: Fernández-Garayzábal
mesh_terms: D042401:Alcaligenaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology; D000835:Animals, Wild / Q000382:microbiology; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D014066:Palatine Tonsil / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011700:Putrescine / Q000031:analogs & derivatives / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013552:Swine / Q000382:microbiology*; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C002952:2-hydroxyputrescine; C030778:ubiquinone 8; D011700:Putrescine