title: Genome Editing in Rats Using TALE Nucleases.
abstract: The rat is an important animal model to understand gene function and model human diseases. Since recent years, the development of gene-specific nucleases has become important for generating new rat models of human diseases, to analyze the role of genes and to generate human antibodies. Transcription activator-like (TALE) nucleases efficiently create gene-specific knockout rats and lead to the possibility of gene targeting by homology-directed recombination (HDR) and generating knock-in rats. We describe a detailed protocol for generating knockout and knock-in rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models.
authors.affiliation: Transgenic Rats Nantes IBiSA - Centre National de Recherche Scientifique, 44093, Nantes, France. laurent.tesson@univ-nantes.fr.|ITUN, CHU Nantes, 30 Bvd J. Monnet, 44093, Nantes, France. laurent.tesson@univ-nantes.fr.|INSERM UMR 1064, Center for Research in Transplantation and Immunology, Nantes, France. laurent.tesson@univ-nantes.fr.
authors.forename: Laurent
authors.initials: L
authors.lastname: Tesson
authors.affiliation: Transgenic Rats Nantes IBiSA - Centre National de Recherche Scientifique, 44093, Nantes, France.|ITUN, CHU Nantes, 30 Bvd J. Monnet, 44093, Nantes, France.|INSERM UMR 1064, Center for Research in Transplantation and Immunology, Nantes, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Remy
authors.affiliation: Transgenic Rats Nantes IBiSA - Centre National de Recherche Scientifique, 44093, Nantes, France.|ITUN, CHU Nantes, 30 Bvd J. Monnet, 44093, Nantes, France.|INSERM UMR 1064, Center for Research in Transplantation and Immunology, Nantes, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Ménoret
authors.affiliation: Transgenic Rats Nantes IBiSA - Centre National de Recherche Scientifique, 44093, Nantes, France.|ITUN, CHU Nantes, 30 Bvd J. Monnet, 44093, Nantes, France.|INSERM UMR 1064, Center for Research in Transplantation and Immunology, Nantes, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Usal
authors.affiliation: Transgenic Rats Nantes IBiSA - Centre National de Recherche Scientifique, 44093, Nantes, France.|ITUN, CHU Nantes, 30 Bvd J. Monnet, 44093, Nantes, France.|INSERM UMR 1064, Center for Research in Transplantation and Immunology, Nantes, France.
authors.forename: Reynald
authors.initials: R
authors.lastname: Thinard
authors.affiliation: Transgenic Rats Nantes IBiSA - Centre National de Recherche Scientifique, 44093, Nantes, France.|ITUN, CHU Nantes, 30 Bvd J. Monnet, 44093, Nantes, France.|INSERM UMR 1064, Center for Research in Transplantation and Immunology, Nantes, France.
authors.forename: Chloé
authors.initials: C
authors.lastname: Savignard
authors.affiliation: INSERM U1154, CNRS UMR7196, Museum National d'Histoire Naturelle, 43 rue Cuvier, 75005, Paris, France.
authors.forename: Anne
authors.initials: A
authors.lastname: De Cian
authors.affiliation: INSERM U1154, CNRS UMR7196, Museum National d'Histoire Naturelle, 43 rue Cuvier, 75005, Paris, France.
authors.forename: Carine
authors.initials: C
authors.lastname: Giovannangeli
authors.affiliation: INSERM U1154, CNRS UMR7196, Museum National d'Histoire Naturelle, 43 rue Cuvier, 75005, Paris, France.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Concordet
authors.affiliation: Transgenic Rats Nantes IBiSA - Centre National de Recherche Scientifique, 44093, Nantes, France.|ITUN, CHU Nantes, 30 Bvd J. Monnet, 44093, Nantes, France.|INSERM UMR 1064, Center for Research in Transplantation and Immunology, Nantes, France.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Anegon
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D004720:Endonucleases / Q000235:genetics*; D055786:Gene Knockout Techniques / Q000379:methods*; D016678:Genome; D059765:Homologous Recombination / Q000235:genetics; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015534:Trans-Activators / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D015534:Trans-Activators; D004720:Endonucleases
keywords: Gene editing; Genetic engineering; Genomics; Homology-directed repair; Hprt; Knock-in; Knockout; Nonhomologous end joining; Rat model; Rosa26; TALE nucleases; TALEN; Targeted integration