title: MicroRNA-92a promotes growth, metastasis, and chemoresistance in non-small cell lung cancer cells by targeting PTEN.
abstract: MicroRNA-92a (miR-92a) has been reported to play important roles in tumorigenesis of human various cancers. However, the roles and underlying molecular mechanism of miR-92a in non-small cell lung cancer (NSCLC) have not been totally elucidated. Therefore, the aims of this study were to determine the role of miR-92a and to elucidate its regulatory mechanism in NSCLC. We found that miR-92a was significantly upregulated in NSCLC tissues compared to matched adjacent normal lung tissues, and its expression is significantly associated with clinical characteristics of patients, including tumor, node, and metastasis (TNM) stage; tumor size; and lymph node metastasis (all P < 0.01). Function assays demonstrated that upregulation of miR-92a in NSCLC cells promoted cell proliferation, migration, and invasion, decreased apoptosis and caspase-3 activity, and enhanced chemoresistance of NSCLC cells, whereas downregulation of miR-92a showed the opposite effects. Moreover, phosphatase and tensin homolog (PTEN), a unique tumor suppressor gene, was confirmed as a direct target of miR-92a, and PTEN messenger RNA (mRNA) expression was decreased in NSCLC tissues and was inversely correlated with miR-92a. Downregulation of PTEN could mimic the same effects of miR-92a mimic in NSCLC cells and rescue the effects on NSCLC cells induced by miR-92a inhibitor. Taken together, these findings suggested that miR-92a could promote growth, metastasis, and chemoresistance in NSCLC cells at least partially by targeting PTEN.
authors.affiliation: Department of Thoracic Surgery, The First Hospital, Jilin University, 71 Xinming Street, Chaoyang District, Chang Chun, 130021, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ren
authors.affiliation: Department of Thoracic Surgery, The First Hospital, Jilin University, 71 Xinming Street, Chaoyang District, Chang Chun, 130021, China.
authors.forename: Fangchao
authors.initials: F
authors.lastname: Gong
authors.affiliation: Department of Thoracic Surgery, The First Hospital, Jilin University, 71 Xinming Street, Chaoyang District, Chang Chun, 130021, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Thoracic Surgery, The First Hospital, Jilin University, 71 Xinming Street, Chaoyang District, Chang Chun, 130021, China.
authors.forename: Jindong
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Thoracic Surgery, The First Hospital, Jilin University, 71 Xinming Street, Chaoyang District, Chang Chun, 130021, China. zhanghong19678@126.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000072283:A549 Cells; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D009362:Neoplasm Metastasis; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
chemical_list: D020413:3' Untranslated Regions; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase
keywords: Chemotherapy; MicroRNA-92a; Non-small cell lung cancer; PTEN