title: Evaluation of (99m)Tc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in mice.
abstract: Pancreatic adenocarcinoma is important in oncology because of its high mortality rate. Deaths may be avoided if an early diagnosis could be achieved. Several types of tumors overexpress gastrin-releasing peptide receptors (GRPr), including pancreatic cancer cells. Thus, a radiolabeled peptide derivative of gastrin-releasing peptide (GRP) may be useful as a specific imaging probe. The purpose of the present study was to evaluate the feasibility of using (99m)Tc-HYNIC-βAla-Bombesin(7-14)as an imaging probe for Capan-1 pancreatic adenocarcinoma. Xenographic pancreatic tumor was developed in nude mice and characterized by histopathological analysis. Biodistribution studies and scintigraphic images were carried out in tumor-bearing nude mice. The two methods showed higher uptake by pancreatic tumor when compared to muscle (used as control), and the tumor-to-muscle ratio indicated that (99m)Tc-HYNIC-βAla-Bombesin (7-14)uptake was four-fold higher in tumor cells than in other tissues. Scintigraphic images also showed a clear signal at the tumor site. The present data indicate that (99m)Tc-HYNIC-βAla-Bombesin (7-14) may be useful for the detection of pancreatic adenocarcinoma.
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
authors.forename: F N
authors.initials: FN
authors.lastname: Carlesso
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
authors.forename: L L
authors.initials: LL
authors.lastname: Fuscaldi
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
authors.forename: R S
authors.initials: RS
authors.lastname: Araújo
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
authors.forename: C S
authors.initials: CS
authors.lastname: Teixeira
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
authors.forename: M C
authors.initials: MC
authors.lastname: Oliveira
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
authors.forename: S O A
authors.initials: SO
authors.lastname: Fernandes
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
authors.forename: G D
authors.initials: GD
authors.lastname: Cassali
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
authors.forename: D C
authors.initials: DC
authors.lastname: Reis
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
authors.forename: A L B
authors.initials: AL
authors.lastname: Barros
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BR.
authors.forename: V N
authors.initials: VN
authors.lastname: Cardoso
mesh_terms: D000230:Adenocarcinoma / Q000000981:diagnostic imaging* / Q000473:pathology; D000818:Animals; D001839:Bombesin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D019886:Gastrin-Releasing Peptide / Q000031:analogs & derivatives; D064593:Heterografts / Q000000981:diagnostic imaging / Q000473:pathology; D006801:Humans; D008297:Male; D008819:Mice, Nude; D009132:Muscles / Q000000981:diagnostic imaging; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics*; D010190:Pancreatic Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D010446:Peptide Fragments / Q000493:pharmacokinetics; D011877:Radionuclide Imaging
chemical_list: D015609:Organotechnetium Compounds; D010446:Peptide Fragments; C000606095:technetium 99m HYNIC-beta-Ala-bombesin(7-14); D019886:Gastrin-Releasing Peptide; D001839:Bombesin