title: Host Transcriptional Profiles and Immunopathologic Response following Mycobacterium avium subsp. paratuberculosis Infection in Mice.
abstract: Paratuberculosis or Johne's disease is a chronic granulomatous enteropathy in ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP) infection. In the present study, we examined the host response to MAP infection in spleens of mice in order to investigate the host immunopathology accompanying host-pathogen interaction. Transcriptional profiles of the MAP-infected mice at 3 and 6 weeks p.i. showed severe histopathological changes, whereas those at 12 weeks p.i. displayed reduced lesion severity in the spleen and liver. MAP-infected mice at 3 and 6 weeks p.i. showed up-regulation of interferon-related genes, scavenger receptor, and complement components, suggesting an initial innate immune reaction, such as macrophage activation, bactericidal activity, and macrophage invasion of MAP. Concurrently, MAP-infected mice at 3 and 6 weeks p.i. were also suggested to express M2 macrophage phenotype with up-regulation of Mrc1, and Marco and down-regulation of MHC class II, Ccr7, and Irf5, and canonical pathways related to the T cell response including ICOS-ICOSL signaling in T helper cells, calcium-induced T lymphocyte apoptosis, and CD28 signaling in T helper cell. These results provide information which furthers the understanding of the immunopathologic response to MAP infection in mice, thereby providing insights valuable for research into the pathogenesis for MAP infection.
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, Korea; Department of Microbiology, Gyeonsang National University School of Medicine, Jinju, Korea.
authors.forename: Min-Kyoung
authors.initials: MK
authors.lastname: Shin
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Hongtae
authors.initials: H
authors.lastname: Park
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Shin
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Myunghwan
authors.initials: M
authors.lastname: Jung
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Su-Hyung
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Dae-Yong
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, Korea; Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang, Korea.
authors.forename: Han Sang
authors.initials: HS
authors.lastname: Yoo
mesh_terms: D000818:Animals; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology*; D054884:Host-Pathogen Interactions / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D010283:Paratuberculosis / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology