title: NFκB-p50 as a blood based protein marker for early diagnosis and prognosis of head and neck squamous cell carcinoma.
abstract: Head and neck squamous cell carcinoma (HNSCC) is the major health concern in Indian population. Despite of advanced treatment the mortality rate for this disease has not been improved very much. Current research focused on development of protein marker for the diagnosis and prognosis of HNSCC. The case control study was performed with 125 HNSCC patients and 104 control cases. The level of p50 and IκBα proteins in serum were evaluated at pre and post therapy by label free real time surface plasmon resonance (SPR) and western blot analysis. The serum p50 concentration were significantly (P < 0.0001) higher at the time of diagnosis i.e. pre therapy (Mean ± SD = 27.06 ± 4.88 ng/μl) as compared to controls (Mean ± SD = 16.96 ± 4.04 ng/μl) while it decline at post therapy (Mean ± SD = 21.01 ± 4.98 ng/μl). Similarly, the concentration of IκBα protein in serum were slightly higher at pre therapy (Mean ± SD = 8.33 ± 1.85 ng/μl) as compared to controls (Mean ± SD = 7.27 ± 1.84 ng/μl) and declined at post therapy (Mean ± SD = 7.09 ± 1.24 ng/μl). The level of p50 was also high at the early stage of the disease. The specificity and sensitivity of p50 proteins obtained from ROC analysis revealed the potentiality to be diagnostic protein marker for HNSCC for its accuracy in the study cohort.
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Vishal
authors.initials: V
authors.lastname: Sahu
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Vertica
authors.initials: V
authors.lastname: Agnihotri
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Amrendra Pratap
authors.initials: AP
authors.lastname: Singh
authors.affiliation: Department of Radiation Oncology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Bhasker
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India. Electronic address: sharmistha_d@hotmail.com.
authors.forename: Sharmistha
authors.initials: S
authors.lastname: Dey
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D042241:Early Diagnosis; D005260:Female; D015870:Gene Expression; D006258:Head and Neck Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D006801:Humans; D020672:I-kappa B Proteins / Q000097:blood / Q000235:genetics; D008297:Male; D008875:Middle Aged; D000072000:NF-KappaB Inhibitor alpha; D052002:NF-kappa B p50 Subunit / Q000097:blood* / Q000235:genetics; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D012372:ROC Curve; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D014408:Biomarkers, Tumor; D020672:I-kappa B Proteins; D052002:NF-kappa B p50 Subunit; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha
keywords: Cancer; NF-κB; SPR; Serum