title: Targeted Gene Delivery to Macrophages by Biodegradable Star-Shaped Polymers.
abstract: In this report, two biodegradable star-shaped polyasparamide derivatives and four analogues modified with either mannose or folic acid moiety for preferential targeting of a difficult-to-transfect immune cell type, i.e., macrophage, have been synthesized. Each of the prepared star polymers complexes with plasmid DNA to form nanosized particles featuring a core-shell-like morphology. Mannose or folate functionalized star polymers can greatly improve the transfection performance on a macrophage cell line RAW 264.7. As a result, a combination of targeting ligand modification and topological structures of gene carriers is a promising strategy for immune cells-based gene therapy.
authors.affiliation: Department of Polymer Science & Engineering, School of Chemistry & Chemical Engineering, Nanjing University , Nanjing 210093, P. R. China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center of Nanjing University and National Resource Center for Mutant Mice , Nanjing 210061, P. R. China.
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Polymer Science & Engineering, School of Chemistry & Chemical Engineering, Nanjing University , Nanjing 210093, P. R. China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, Baylor College of Medicine , Houston, Texas 77030, United States.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center of Nanjing University and National Resource Center for Mutant Mice , Nanjing 210061, P. R. China.
authors.forename: Dejing
authors.initials: D
authors.lastname: Pan
authors.affiliation: MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center of Nanjing University and National Resource Center for Mutant Mice , Nanjing 210061, P. R. China.
authors.forename: Jianghuai
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Polymer Science & Engineering, School of Chemistry & Chemical Engineering, Nanjing University , Nanjing 210093, P. R. China.
authors.forename: Fude
authors.initials: F
authors.lastname: Feng
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000737:chemistry; D005492:Folic Acid / Q000737:chemistry*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D008358:Mannose / Q000737:chemistry*; D051379:Mice; D010957:Plasmids / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
chemical_list: D011108:Polymers; D004247:DNA; D005492:Folic Acid; D008358:Mannose
keywords: core−shell; macrophage; ring opening polymerization; star polymer; targeted gene delivery