title: Effects of combined ferrous sulfate administration and exposure to static magnetic field on brain oxidative stress and emotional behavior.
abstract: The present study was done to investigate behavioral effects and oxidative stress in iron- treated and co-exposed static magnetic field (SMF)-iron rats. Anxiety in the elevated plus- maze test, and motor skills were also assessed in the stationary beam and suspended string tests. After behavioral tests, the rats were anesthetized and their brains were removed for biochemical analysis. The co-exposure to iron and SMF induced a significant difference in elevated plus-maze test in rats. The frequency of entries and time spent in the open arms was significantly reduced (p<0.05) in the iron- and SMF-exposed group compared with the group treated with iron alone and in the control group. However, no significant difference was noticed for the motor skill test between the three groups. The biochemical investigation showed that malondialdehyde level increased (p<0.001) and that glutathione level and catalase enzyme activity decreased (p<0.001) in brain of iron- and SMF-exposed group. The dose of iron alone used in present study, was unable to induce any effect. However, the 128 mT SMF in the presence of iron ions in the body can induce disruption in the emotional behavior and can produce oxidative stress in brain tissue of rats.
authors.affiliation: Université de Carthage, Faculté des Sciences de Bizerte, Laboratoire de Physiologie Intégrée, Jarzouna, Tunisia. Email: Miryam.Elferchichi@fsb.rnu.tn.
authors.forename: Miryam
authors.initials: M
authors.lastname: Elferchichi
authors.forename: Karima
authors.initials: K
authors.lastname: Maaroufi
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ammari
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sakly
authors.forename: Hafedh
authors.initials: H
authors.lastname: Abdelmelek
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001007:Anxiety / Q000523:psychology; D001923:Brain Chemistry / Q000187:drug effects* / Q000528:radiation effects*; D004644:Emotions / Q000187:drug effects* / Q000528:radiation effects*; D005296:Ferrous Compounds / Q000494:pharmacology*; D060526:Magnetic Fields; D008297:Male; D053580:Muscle Strength / Q000187:drug effects / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D004856:Postural Balance / Q000187:drug effects / Q000528:radiation effects; D011597:Psychomotor Performance / Q000187:drug effects / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D005296:Ferrous Compounds; C020748:ferrous sulfate