title: Effect of Liuweidihuang pill and Jinkuishenqi pill on inhibition of spontaneous breast carcinoma growth in mice.
abstract: OBJECTIVE
  To investigate the preventing and treating action of Liuweidihuang pill (LP) and Jinkuishenqi pill (JP) on spontaneous breast carcinoma in mice.
  
  
  METHODS
  A model of spontaneous breast carcinoma was derived from 11.5-month-old female Kunming breeding mice following the delivery of several litters. The mice were randomly divided into five groups: model control group (C), Liuweidihuang pill high-dose group (LH; 4.6 g · kg(-1) · d(-1)), Liuweidihuang pill low-dose group (LL; 2.3 g · kg(-1) · d(-1)), Jinkuishenqi pill high-dose group (JH; 4.6 g · kg(-1) · d(-1)) and Jinkuishenqi pill low-dose group (JL; 2.3 g · kg(-1) · d(-1)). Cancer tissue volume was measured by water immersion. Histopathology was analyzed by hematoxylin and eosin staining. Vascular endothelial growth factor (VEGF), extracellular signal-regulated kinase (ERK) and cyclin D1 protein expression in cancer tissue was assayed by western blotting.
  
  
  RESULTS
  Compared with the control group, cancer tissue volume and weight were lower in the LP and JP groups, and survival time was longer. The expression of VEGF, ERK and Cyclin D1 were inhibited in the LP and JP groups (P < 0.05), and cell differentiation was increased. Tumor weights and volumes and VEGF, ERK and Cyclin D1 expression in LL or LH were significantly lower than in JL and JH (P < 0.01).
  
  
  CONCLUSIONS
  Both LP and JP could restrain cancer growth and promote cancer cell differentiation; moreover, LP was more effective than JP The likely mechanism of action was via inhibition of VEGF, ERK and cyclin D1.
authors.forename: Lixiang
authors.initials: L
authors.lastname: Zheng
authors.forename: Hongning
authors.initials: H
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Gong
authors.forename: Xianming
authors.initials: X
authors.lastname: Meng
authors.forename: Runde
authors.initials: R
authors.lastname: Jiang
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Wang
authors.forename: Qiaofeng
authors.initials: Q
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D049109:Cell Proliferation / Q000187:drug effects*; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D051379:Mice; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D042461:Vascular Endothelial Growth Factor A; D019938:Cyclin D1; D048049:Extracellular Signal-Regulated MAP Kinases