title: Luteolin induces caspase-14-mediated terminal differentiation in human epidermal keratinocytes.
abstract: Recent studies have demonstrated the role of caspase-14 in terminally differentiated keratinocytes, and its expression may decrease the magnitude of tumors in the epidermis. In the present study, we assessed the potential of luteolin (LUT) to elicit the expression of caspase-14 in terminal differentiation of human keratinocytes. The semi-qualitative RT-PCR data revealed a significant level of caspase-14 expression in LUT-treated human immortalized keratinocytes (HaCaT) with respect to untreated cells. The quantitative data (ELISA) further supported the potency of LUT to induce caspase-14 expression at 3.19 ng/ml when compared to 1.29 ng/ml of vitamin D3 (positive control). Further, the enhanced expression of human involucrin gene in LUT-treated HaCaT cells confirmed its ability to drive terminal differentiation in these cells. These preliminary results provide first-hand information about the in vitro potential of LUT to elicit the expression of caspase-14, thereby inducing terminal differentiation in human keratinocytes.
authors.affiliation: Department of Cellular and Molecular Biology, UTHSCT, Tyler, TX, USA.|School of Bio Sciences and Technology, VIT University, Vellore, India.
authors.forename: V Cijo
authors.initials: VC
authors.lastname: George
authors.affiliation: Department of Clinical Microbiology, CMC, Vellore, India.|School of Bio Sciences and Technology, VIT University, Vellore, India.
authors.forename: D R Naveen
authors.initials: DR
authors.lastname: Kumar
authors.affiliation: School of Bio Sciences and Technology, VIT University, Vellore, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Suresh
authors.affiliation: Department of Zoology, Government Arts College, Dharmapuri, 636705, Tamil Nadu, India. ashoku_2000@yahoo.com.
authors.forename: R Ashok
authors.initials: RA
authors.lastname: Kumar
mesh_terms: D053456:Caspase 14 / Q000096:biosynthesis* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002762:Cholecalciferol / Q000494:pharmacology; D000078404:Epidermal Cells; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D047311:Luteolin / Q000494:pharmacology*; D011498:Protein Precursors / Q000096:biosynthesis
chemical_list: D011498:Protein Precursors; D002762:Cholecalciferol; C032253:involucrin; D053456:Caspase 14; D047311:Luteolin
keywords: Caspase-14; Human keratinocytes; Luteolin; Terminal differentiation