title: Anticancer Activity of Atractylodes lancea (Thunb.) DC in a Hamster Model and Application of PET-CT for Early Detection and Monitoring Progression of Cholangiocarcinoma.
abstract: Opisthorchis viverrini (OV)-induced cholangiocarcinoma (CCA) is an important cancer in the Great Mekong region, particularly in Thailand. Limitations of treatment options and the lack of an effective diagnostic tool for early detection of CCA are major concerns for the control of this type of cancer. The aim of the study was to investigate anti-CCA activity of the ethanolic extract of Atractylodes lancea (Thunb.) DC., and the applicability of positron emission tomography-computed tomography (PET-CT) as a tool for detection and monitoring the progression of CCA in Opisthorchis viverrini (OV)/dimethylnitrosamine (DMN)-induced CCA hamsters. Male Syrian hamsters were used for toxicity tests and anti-CCA activity evaluation. Development of CCA was induced by initial feeding of 50 metacercariae of OV, followed by drinking water containing 12.5 ppm of DMN in hamsters. The ethanolic extract of A. lancea (Thunb.) DC. was administered orally for 30 days. PET-CT was performed every 4 weeks after initiation of CCA using 18F-fluorodeoxyglucose (18F-FDG). Results from the present study suggest that the ethanolic extract of A. lancea (Thunb.) DC. rhizome exhibited promising anti-CCA activity and safety profile in the OV/DMN-induced hamster model. To successfully apply PET-CT as a tool for early detection of tumor development and progression, modification of radiolabeling approach is required to improve its specificity for CCA cells.
authors.affiliation: Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Chulabhorn International College of Medicine, Bangkok, Thailand E-mail : kesaratmu@yahoo.com.
authors.forename: Tullayakorn
authors.initials: T
authors.lastname: Plengsuriyakarn
authors.forename: Naoki
authors.initials: N
authors.lastname: Matsuda
authors.forename: Juntra
authors.initials: J
authors.lastname: Karbwang
authors.forename: Vithoon
authors.initials: V
authors.lastname: Viyanant
authors.forename: Kenji
authors.initials: K
authors.lastname: Hirayama
authors.forename: Kesara
authors.initials: K
authors.lastname: Na-Bangchang
mesh_terms: D000818:Animals; D031174:Atractylodes / Q000633:toxicity; D001650:Bile Duct Neoplasms / Q000000981:diagnostic imaging / Q000188:drug therapy*; D018281:Cholangiocarcinoma / Q000000981:diagnostic imaging / Q000188:drug therapy*; D006224:Cricetinae; D018450:Disease Progression; D055088:Early Detection of Cancer / Q000379:methods; D005260:Female; D019788:Fluorodeoxyglucose F18; D008297:Male; D008647:Mesocricetus; D064847:Multimodal Imaging; D008517:Phytotherapy / Q000009:adverse effects; D010936:Plant Extracts / Q000627:therapeutic use* / Q000633:toxicity; D049268:Positron-Emission Tomography; D014057:Tomography, X-Ray Computed
chemical_list: D010936:Plant Extracts; D019788:Fluorodeoxyglucose F18