title: Identification of STAT3 and STAT5 proteins in the rat suprachiasmatic nucleus and the Day/Night difference in astrocytic STAT3 phosphorylation in response to lipopolysaccharide.
abstract: Signal transducers and activators of transcription (STAT) proteins regulate many aspects of cellular physiology from growth and differentiations to immune responses. Using immunohistochemistry, we show the daily rhythm of STAT3 protein in the rat suprachiasmatic nucleus (SCN), with low but significant amplitude peaking in the morning. We also reveal the strong expression of STAT5A in astrocytes of the SCN and the STAT5B signal in nonastrocytic cells. Administration of lipopolysaccharide (LPS) acutely induced phosphorylation of STAT3 on Tyr705 during both the day and the night and induced phosphorylation on Ser727 but only after the daytime application. The LPS-induced phospho-STAT3 (Tyr705) remained elevated for 24 hr after the daytime application but declined within 8 hr when LPS was applied at night.
authors.affiliation: Department of Physiology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
authors.forename: Simona
authors.initials: S
authors.lastname: Moravcová
authors.affiliation: Department of Physiology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Červená
authors.affiliation: Department of Physiology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
authors.forename: Dominika
authors.initials: D
authors.lastname: Pačesová
authors.affiliation: Department of Physiology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
authors.forename: Zdeňka
authors.initials: Z
authors.lastname: Bendová
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D050799:STAT5 Transcription Factor / Q000378:metabolism*; D013493:Suprachiasmatic Nucleus / Q000166:cytology* / Q000187:drug effects*; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D005904:Glial Fibrillary Acidic Protein; D008070:Lipopolysaccharides; D050796:STAT3 Transcription Factor; D050799:STAT5 Transcription Factor; D014446:Tyrosine 3-Monooxygenase
keywords: STAT proteins; circadian clock; lipopolysaccharide; rat; suprachiasmatic nucleus