title: Impact of early developmental fluoride exposure on the peripheral pain sensitivity in mice.
abstract: Consumption of high concentration of fluoride in the drinking water would cause the fluorosis and chronic pain. Similar pain syndrome appeared in the patients in fluoride therapy of osteoporotic. The aim of the current study was to examine whether exposing immature mice to fluoride would modify the peripheral pain sensitivity or even cause a pain syndrome. We gave developmental fluoride exposure to mice in different concentration (0mg/L, 50mg/L and 100mg/L) and evaluated their basal pain threshold. Von Frey hair test, hot plate test and formalin test were conducted to examine the mechanical, thermal nociceptive threshold and inflammatory pain, respectively. In addition, the expression of hippocampal brain-derived neurotrophic factor (BDNF) was also evaluated by Western blotting. Hyperalgesia in fluoride exposure mice was exhibited in the Von Frey hair test, hot plate test and formalin test. Meanwhile, the expression of BDNF was significantly higher than that of control group. The results suggest that early developmental fluoride exposure may lower the basal pain threshold and be associated with the increasing of BDNF expression in hippocampus.
authors.affiliation: Affiliated Stomatology Hospital of Xi'an Jiaotong University Health Science Center, Xi'an 710004, Shaanxi, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.affiliation: Affiliated Stomatology Hospital of Xi'an Jiaotong University Health Science Center, Xi'an 710004, Shaanxi, PR China; College of Medicine & Forensics, Xi'an Jiaotong University Health Science Center, Xi'an 710061, Shaanxi, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: College of Medicine & Forensics, Xi'an Jiaotong University Health Science Center, Xi'an 710061, Shaanxi, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Psychiatry, First Affiliated Hospital of Xi'an Jiaotong, University College of Medicine, Xi'an, Shaanxi, PR China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Dong
authors.affiliation: College of Medicine & Forensics, Xi'an Jiaotong University Health Science Center, Xi'an 710061, Shaanxi, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Chu
authors.affiliation: Affiliated Stomatology Hospital of Xi'an Jiaotong University Health Science Center, Xi'an 710004, Shaanxi, PR China. Electronic address: tiezhou@mail.xjtu.edu.cn.
authors.forename: Tie-Zhou
authors.initials: TZ
authors.lastname: Hou
authors.affiliation: College of Medicine & Forensics, Xi'an Jiaotong University Health Science Center, Xi'an 710061, Shaanxi, PR China; Key Laboratory of the Health Ministry for Forensic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an 710061, Shaanxi, PR China; Key Laboratory of Environment and Genes Related to Diseases of the Education Ministry, Xi'an Jiaotong University Health Science Center, Xi'an 710061, Shaanxi, PR China. Electronic address: psydyh@mail.xjtu.edu.cn.
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Dang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D001835:Body Weight / Q000187:drug effects; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002327:Cariostatic Agents / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005106:Exploratory Behavior / Q000187:drug effects; D005459:Fluorides / Q000633:toxicity*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D006930:Hyperalgesia / Q000503:physiopathology; D008124:Locomotion / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010146:Pain / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology; D010147:Pain Measurement; D017288:Pain Threshold / Q000187:drug effects*; D011930:Reaction Time / Q000187:drug effects; D013997:Time Factors
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D002327:Cariostatic Agents; D005459:Fluorides
keywords: BDNF; Developmental exposure; Fluoride; Mice; Pain sensitivity