title: The Effects of GH Transgenic Goats on the Microflora of the Intestine, Feces and Surrounding Soil.
abstract: The development of genetically engineered animals has brought with it increasing concerns about biosafety issues. We therefore evaluated the risks of growth hormone from transgenic goats, including the probability of horizontal gene transfer and the impact on the microbial community of the goats' gastrointestinal tracts, feces and the surrounding soil. The results showed that neither the GH nor the neoR gene could be detected in the samples. Moreover, there was no significant change in the microbial community of the gastrointestinal tracts, feces and soil, as tested with PCR-denaturing gradient gel electrophoresis and 16S rDNA sequencing. Finally, phylogenetic analysis showed that the intestinal content, feces and soil samples all contained the same dominant group of bacteria. These results demonstrated that expression of goat growth hormone in the mammary of GH transgenic goat does not influence the microflora of the intestine, feces and surrounding soil.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: Zekun
authors.initials: Z
authors.lastname: Bao
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Lin
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Hu
authors.affiliation: Shanghai Transgenic Research Center, 88 Cai-Lun Road, Shanghai, 201210, People's Republic of China.
authors.forename: Huiqing
authors.initials: H
authors.lastname: Yu
authors.affiliation: Shanghai Transgenic Research Center, 88 Cai-Lun Road, Shanghai, 201210, People's Republic of China.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Yu
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000382:microbiology; D001419:Bacteria / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D006041:Goats / Q000235:genetics* / Q000382:microbiology*; D013006:Growth Hormone / Q000235:genetics*; D007422:Intestines / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil; D013006:Growth Hormone