title: Glycoprotein B7-H3 overexpression and aberrant glycosylation in oral cancer and immune response.
abstract: The incidence and mortality rate of oral cancer continue to rise, partly due to the lack of effective early diagnosis and increasing environmental exposure to cancer-causing agents. To identify new markers for oral cancer, we used a sialylation probe to investigate the glycoproteins differentially expressed on oral cancer cells. Of the glycoproteins identified, B7 Homolog 3 (B7-H3) was significantly overexpressed in oral squamous cell carcinoma (OSCC), and its overexpression correlated with larger tumor size, advanced clinical stage, and low survival rate in OSCC patients. In addition, knockdown of B7-H3 suppressed tumor cell proliferation, and restoration of B7-H3 expression enhanced tumor growth. It was also found that the N-glycans of B7-H3 from Ca9-22 oral cancer cells contain the terminal α-galactose and are more diverse with higher fucosylation and better interaction with DC-SIGN [DC-specific intercellular adhesion molecule-3 (ICAM-3)-grabbing nonintegrin] and Langerin on immune cells than that from normal cells, suggesting that the glycans on B7-H3 may also play an important role in the disease.
authors.affiliation: Ph.D. Program in Translational Medicine, National Taiwan University and Academia Sinica, Taipei 100, Taiwan; Genomic Research Center, Academia Sinica, Taipei 115, Taiwan; Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei 100, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei 100, Taiwan;
authors.forename: Jung-Tsu
authors.initials: JT
authors.lastname: Chen
authors.affiliation: Genomic Research Center, Academia Sinica, Taipei 115, Taiwan;
authors.forename: Chein-Hung
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei 100, Taiwan;
authors.forename: Ko-Li
authors.initials: KL
authors.lastname: Ku
authors.affiliation: Genomic Research Center, Academia Sinica, Taipei 115, Taiwan;
authors.forename: Michael
authors.initials: M
authors.lastname: Hsiao
authors.affiliation: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei 100, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei 100, Taiwan; Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei 100, Taiwan;
authors.forename: Chun-Pin
authors.initials: CP
authors.lastname: Chiang
authors.affiliation: Genomic Research Center, Academia Sinica, Taipei 115, Taiwan;
authors.forename: Tsui-Ling
authors.initials: TL
authors.lastname: Hsu
authors.affiliation: Ph.D. Program in Translational Medicine, National Taiwan University and Academia Sinica, Taipei 100, Taiwan; Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei 100, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei 100, Taiwan; Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei 100, Taiwan chwong@gate.sinica.edu.tw minhueychen@ntu.edu.tw.
authors.forename: Min-Huey
authors.initials: MH
authors.lastname: Chen
authors.affiliation: Ph.D. Program in Translational Medicine, National Taiwan University and Academia Sinica, Taipei 100, Taiwan; Genomic Research Center, Academia Sinica, Taipei 115, Taiwan; chwong@gate.sinica.edu.tw minhueychen@ntu.edu.tw.
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
mesh_terms: D060887:B7 Antigens / Q000276:immunology / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D006031:Glycosylation; D006801:Humans; D009062:Mouth Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
chemical_list: D060887:B7 Antigens; C423237:CD276 protein, human
keywords: CD276; fucose; glycan sequencing; proliferation; terminal α-galactose