title: HMGCR positively regulated the growth and migration of glioblastoma cells.
abstract: The metabolic program of cancer cells is significant different from the normal cells, which makes it possible to develop novel strategies targeting cancer cells. Mevalonate pathway and its rate-limiting enzyme HMG-CoA reductase (HMGCR) have shown important roles in the progression of several cancer types. However, their roles in glioblastoma cells remain unknown. In this study, up-regulation of HMGCR in the clinical glioblastoma samples was observed. Forced expression of HMGCR promoted the growth and migration of U251 and U373 cells, while knocking down the expression of HMGCR inhibited the growth, migration and metastasis of glioblastoma cells. Molecular mechanism studies revealed that HMGCR positively regulated the expression of TAZ, an important mediator of Hippo pathway, and the downstream target gene connective tissue growth factor (CTGF), suggesting HMGCR might activate Hippo pathway in glioblastoma cells. Taken together, our study demonstrated the oncogenic roles of HMGCR in glioblastoma cells and HMGCR might be a promising therapeutic target.
authors.affiliation: Department of Neurosurgery, Central Hospital of Zhuzhou, Zhuzhou, Hunan Province 412000, PR China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: Department of Neurosurgery, Central Hospital of Zhuzhou, Zhuzhou, Hunan Province 412000, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong Province 510120, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong Province 510120, PR China.
authors.forename: Chenzhi
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Neurosurgery, Central Hospital of Zhuzhou, Zhuzhou, Hunan Province 412000, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Central Hospital of Zhuzhou, Zhuzhou, Hunan Province 412000, PR China.
authors.forename: Yongkai
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Neurosurgery, Central Hospital of Zhuzhou, Zhuzhou, Hunan Province 412000, PR China. Electronic address: hdqmail@163.com.
authors.forename: Deqing
authors.initials: D
authors.lastname: Han
authors.affiliation: State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong Province 510120, PR China; Department of Neurology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong Province 510120, PR China. Electronic address: qinghui@gird.cn.
authors.forename: Qinghui
authors.initials: Q
authors.lastname: Huang
mesh_terms: D000217:Acyltransferases; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism; D005909:Glioblastoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D057809:HEK293 Cells; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics / Q000378:metabolism*; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C526317:CCN2 protein, human; D009363:Neoplasm Proteins; D014157:Transcription Factors; D055513:Connective Tissue Growth Factor; C553220:HMGCR protein, human; D006903:Hydroxymethylglutaryl CoA Reductases; D000217:Acyltransferases; C108675:TAFAZZIN protein, human
keywords: Cell growth and migration; Glioblastoma; HMGCR; Hippo pathway