title: Fluorescent Reporter Libraries as Useful Tools for Optimizing Microbial Cell Factories: A Review of the Current Methods and Applications.
abstract: The use of genetically encoded fluorescent reporters allows speeding up the initial optimization steps of microbial bioprocesses. These reporters can be used for determining the expression level of a particular promoter, not only the synthesis of a specific protein but also the content of intracellular metabolites. The level of protein/metabolite is thus proportional to a fluorescence signal. By this way, mean expression profiles of protein/metabolites can be determined non-invasively at a high-throughput rate, allowing the rapid identification of the best producers. Actually, different kinds of reporter systems are available, as well as specific cultivation devices allowing the on-line recording of the fluorescent signal. Cell-to-cell variability is another important phenomenon that can be integrated into the screening procedures for the selection of more efficient microbial cell factories.
authors.affiliation: Microbial Processes and Interactions (MiPI), Gembloux Agro-Bio Tech, University of Liège , Gembloux , Belgium.
authors.forename: Frank
authors.initials: F
authors.lastname: Delvigne
authors.affiliation: Microbial Processes and Interactions (MiPI), Gembloux Agro-Bio Tech, University of Liège , Gembloux , Belgium.
authors.forename: Hélène
authors.initials: H
authors.lastname: Pêcheux
authors.affiliation: Microbial Processes and Interactions (MiPI), Gembloux Agro-Bio Tech, University of Liège , Gembloux , Belgium.
authors.forename: Cédric
authors.initials: C
authors.lastname: Tarayre
keywords: flow cytometry; high-throughput; mini-bioreactors; single cell