title: Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol.
abstract: The generation of functional hepatocytes is a major challenge for regenerative medicine and drug discovery. Here we show a method that facilitates generation of induced functional hepatocytes (iHeps) from adipose-derived stem cells (ADSCs) within 9 days. iHeps express hepatocytic gene programs and display functions characteristic of mature hepatocytes, including cytochrome P450 enzyme activity. Upon transplantation into mice with carbon tetrachloride (CCl4)-induced acute fulminant liver failure, iHeps restore the liver function and prolong survival. The work could contribute to the development of alternative strategies to obtain nonhepatic cell-derived mature hepatocytes with potential for biomedical and pharmaceutical applications.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. xufen1224@163.com.
authors.forename: Fen
authors.initials: F
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. liujunli89@gmail.com.
authors.forename: Junli
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. dengjie89326@sina.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. tridragon@163.com.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. SCUwangyuan0703@163.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. pcxu1990@sina.com.
authors.forename: Pengchao
authors.initials: P
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. linchengscu@gmail.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. fly109928@163.com.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. 121158277@qq.com.
authors.forename: Fuyi
authors.initials: F
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. yao182@126.com.
authors.forename: Yunqi
authors.initials: Y
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. PrettycatZhang@126.com.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. hmj107@163.com.|Department of Thoracic Oncology, Cancer Center, West China Hospital, Sichuan University, No. 37 Guo Xue Xiang, Chengdu, Sichuan, 610041, P.R. China. hmj107@163.com.
authors.forename: Meijuan
authors.initials: M
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. michael.yu@innoventbio.com.
authors.forename: Dechao
authors.initials: D
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. yuquanwei123hx@163.com.
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, No.17, section3, Renmin South Road, Chengdu, 610041, P.R. China. denghongx@scu.edu.cn.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Deng
mesh_terms: D050153:Abdominal Fat / Q000166:cytology; D053687:Adult Stem Cells / Q000502:physiology*; D000818:Animals; D002251:Carbon Tetrachloride; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D022781:Hepatocytes / Q000502:physiology* / Q000637:transplantation; D017093:Liver Failure / Q000139:chemically induced / Q000628:therapy; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011917:Rats, Inbred Lew
chemical_list: D002251:Carbon Tetrachloride