title: Tracking the activity-dependent diffusion of synaptic proteins using restricted photoconversion of Dendra2.
abstract: Spines are small protrusions on dendritic membranes receiving inputs from axonal termini. They consist in a head connected to the dendritic shaft by a narrow neck and contain multiple synaptic proteins that interact in a coordinated manner to allow for synaptic communication. This process involves many proteins that are moving in and out spines. However, comparing this synaptodendritic movement in basal and stimulated conditions is very challenging. Here we describe an elegant method to measure the activity-dependent diffusion of synaptic proteins using Dendra2 photoconversion. We provide a successful method to obtain Dendra2-photoconverted images and a step-by-step procedure to analyze the data. This live-imaging approach may also apply to investigate the diffusion of proteins across other subcellular compartments or organelles including but not restricted to, nucleus, nucleolus, ER, or vesicular structures. Once the imaging system is set up, data can be acquired in 1-30 min and analyzed in approximately 1-4 h.
authors.affiliation: Centre National de la Recherche Scientifique UMR7275 - Laboratory of Excellence "Network for Innovation on Signal Transduction, Pathways in Life Sciences, " Institut de Pharmacologie Moléculaire et Cellulaire, University of Nice - Sophia Antipolis Valbonne, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Cassé
authors.affiliation: Centre National de la Recherche Scientifique UMR7275 - Laboratory of Excellence "Network for Innovation on Signal Transduction, Pathways in Life Sciences, " Institut de Pharmacologie Moléculaire et Cellulaire, University of Nice - Sophia Antipolis Valbonne, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Martin
keywords: Dendra2; SUMO; live cell imaging; neuronal activity; synapse