title: Comparison of soil microbial communities inhabiting vineyards and native sclerophyllous forests in central Chile.
abstract: Natural ecosystems provide services to agriculture such as pest control, soil nutrients, and key microbial components. These services and others in turn provide essential elements that fuel biomass productivity. Responsible agricultural management and conservation of natural habitats can enhance these ecosystem services. Vineyards are currently driving land-use changes in many Mediterranean ecosystems. These land-use changes could have important effects on the supporting ecosystems services related to the soil properties and the microbial communities associated with forests and vineyard soils. Here, we explore soil bacterial and fungal communities present in sclerophyllous forests and organic vineyards from three different wine growing areas in central Chile. We employed terminal restriction fragment length polymorphisms (T-RFLP) to describe the soil microbial communities inhabiting native forests and vineyards in central Chile. We found that the bacterial community changed between the sampled growing areas; however, the fungal community did not differ. At the local scale, our findings show that fungal communities differed between habitats because fungi species might be more sensitive to land-use change compared to bacterial species, as bacterial communities did not change between forests and vineyards. We discuss these findings based on the sensitivity of microbial communities to soil properties and land-use change. Finally, we focus our conclusions on the importance of naturally derived ecosystem services to vineyards.
authors.affiliation: Facultad de Ciencias Instituto de Ciencias Ambientales y Evolutivas Campus Isla Teja Universidad Austral de Chile Valdivia Chile ; Instituto de Ecología & Biodiversidad (IEB-Chile) Casilla 653 Santiago Chile.
authors.forename: Luis E
authors.initials: LE
authors.lastname: Castañeda
authors.affiliation: Facultad de Ciencias Instituto de Ciencias Ambientales y Evolutivas Campus Isla Teja Universidad Austral de Chile Valdivia Chile ; Instituto de Ecología & Biodiversidad (IEB-Chile) Casilla 653 Santiago Chile.
authors.forename: Karina
authors.initials: K
authors.lastname: Godoy
authors.affiliation: Instituto de Ecología & Biodiversidad (IEB-Chile) Casilla 653 Santiago Chile ; Departamento de Ecología Facultad de Ciencias Biológicas Pontificia Universidad Católica de Chile Alameda 340 Santiago Chile.
authors.forename: Marlene
authors.initials: M
authors.lastname: Manzano
authors.affiliation: Instituto de Ecología & Biodiversidad (IEB-Chile) Casilla 653 Santiago Chile ; Departamento de Ecología Facultad de Ciencias Biológicas Pontificia Universidad Católica de Chile Alameda 340 Santiago Chile ; The Santa Fe Institute Santa Fe New Mexico 87501 ; Laboratorio Internacional de Cambio Global Pontificia Universidad Católica de Chile Alameda 340 Santiago Chile ; Centro Cambio Global UC Pontificia Universidad Católica de Chile Av. Vicuña Mackenna 4860 Santiago Chile.
authors.forename: Pablo A
authors.initials: PA
authors.lastname: Marquet
authors.affiliation: Facultad de Ciencias Instituto de Ciencias Ambientales y Evolutivas Campus Isla Teja Universidad Austral de Chile Valdivia Chile ; Instituto de Ecología & Biodiversidad (IEB-Chile) Casilla 653 Santiago Chile.
authors.forename: Olga
authors.initials: O
authors.lastname: Barbosa
keywords: Microbial community structure; microbial diversity; soil bacteria; soil fungi; terminal restriction fragment length polymorphisms (T‐RFLP)