title: Frequencies of Six (Five Novel) STR Markers Linked to TUSC3 (MRT7) or NSUN2 (MRT5) Genes Used for Homozygosity Mapping of Recessive Intellectual Disability.
abstract: BACKGROUND
  Non-syndromic autosomal recessive intellectual disability (NS-ARID) is an extremely heterogeneous genetic disorder. Therefore, to investigate these genes, more research is required. One approach to investigate the NS-ARID loci is homozygosity mapping which requires appropriate STR markers within or flanking the gene/s of interest. In this research, we aimed to find novel STRs for two common NS-ARID genes (TUSC3 and NSUN2) and, in addition, to identify allele frequencies of those STR markers.
  
  
  METHODS
  The study group included 119 unrelated healthy individuals. STR markers were investigated using the UCSC genome browser web site and SERV software. Genotyping was determined by multiplex PCR. Data were evaluated using Gene Mapper software. Allele frequencies and observed heterozygosity rates were calculated using PowerStatV12. Deviation from Hardy-Weinberg equilibrium and expected heterozygosity were assessed using the DNAView software.
  
  
  RESULTS
  In total, 56 alleles were detected. According to our research, D8TUSC3SU8.3 and D5NSUN2SU0.5 were the most informative STR markers in MRT7 and MRT5 loci, respectively and showed a high percentage of heterozygosity in Iranian population. The observed range of allele frequencies was from 3.4% to 32.4% and 0.8% to 18.9% for MRT5 and MRT7 loci, respectively. Further, we have evaluated other statistical surveys of these STR markers and discovered that all of the six listed STRs were informative and five meet the Hardy-Weinberg equilibrium for the tester group.
  
  
  CONCLUSIONS
  Finding novel STRs, with high allele heterozygosity, is one of the most significant current finding in the present study for the two common NSARID genes. The recognized heterozygosity of these markers make MRT flanking STR markers very efficient to be used in diagnostic medical genetics labs or homozygosity mapping on NS-ARID.
authors.forename: Shirin
authors.initials: S
authors.lastname: Ghadami
authors.forename: Hossein Malek
authors.initials: HM
authors.lastname: Mohammadi
authors.forename: Jamileh
authors.initials: J
authors.lastname: Malbin
authors.forename: Mahboobeh
authors.initials: M
authors.lastname: Masoodifard
authors.forename: Amene Bandehi
authors.initials: AB
authors.lastname: Sarhaddi
authors.forename: Javad
authors.initials: J
authors.lastname: Tavakkoly-Bazzaz
authors.forename: Sirous
authors.initials: S
authors.lastname: Zeinali
mesh_terms: D019295:Computational Biology; D005787:Gene Frequency; D005808:Genes, Recessive; D056726:Genetic Association Studies; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008607:Intellectual Disability / Q000175:diagnosis / Q000235:genetics* / Q000523:psychology; D008565:Membrane Proteins / Q000235:genetics*; D008780:Methyltransferases / Q000235:genetics*; D018895:Microsatellite Repeats; D010641:Phenotype; D012307:Risk Factors; D012984:Software; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D008565:Membrane Proteins; C524338:TUSC3 protein, human; D025521:Tumor Suppressor Proteins; D008780:Methyltransferases; C515610:NSUN2 protein, human