title: The hrp pathogenicity island of Pseudomonas syringae pv. tomato DC3000 is induced by plant phenolic acids.
abstract: Plants produce a wide array of antimicrobial compounds, such as phenolic compounds, to combat microbial pathogens. The hrp PAI is one of the major virulence factors in the plant pathogen, Pseudomonas syringae. A major role of hrp PAI is to disable the plant defense system during bacterial invasion. We examined the influence of phenolic compounds on hrp PAI gene expression at low and high concentrations. There was approximately 2.5 times more hrpA and hrpZ mRNA in PtoDC3000 that was grown in minimal media (MM) supplemented with 10 -M of ortho-coumaric acid than in PtoDC3000 grown in MM alone. On the other hand, a significantly lower amount of hrpA mRNA was observed in bacteria grown in MM supplemented with a high concentration of phenolic compounds. To determine the regulation pathway for hrp PAI gene expression, we performed qRTPCR using gacS, gacA, and hrpS deletion mutants.
authors.affiliation: Department of Integrated Biological Science, College of Natural Sciences, Pusan National University, Busan, 46241, Republic of Korea.
authors.forename: Jun Seung
authors.initials: JS
authors.lastname: Lee
authors.affiliation: R & D, CJ Cheil Jedang Corporation, Seoul, 04560, Republic of Korea.
authors.forename: Hye Ryun
authors.initials: HR
authors.lastname: Ryu
authors.affiliation: Department of Biotechnology and Bioengineering, College of Engineering, Dong-Eui University, Busan, 47340, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Cha
authors.affiliation: Department of Integrated Biological Science, College of Natural Sciences, Pusan National University, Busan, 46241, Republic of Korea. hsubaik@pusan.ac.kr.
authors.forename: Hyung Suk
authors.initials: HS
authors.lastname: Baik
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D003373:Coumaric Acids / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D044404:Genomic Islands / Q000187:drug effects* / Q000235:genetics*; D062385:Hydroxybenzoates / Q000378:metabolism / Q000494:pharmacology*; D018551:Solanum lycopersicum / Q000378:metabolism / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D010957:Plasmids / Q000235:genetics; D044224:Pseudomonas syringae / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D017384:Sequence Deletion; D014774:Virulence / Q000187:drug effects / Q000235:genetics
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003373:Coumaric Acids; D062385:Hydroxybenzoates; D012333:RNA, Messenger; C087295:HrpZ protein, Pseudomonas syringae; C017616:phenolic acid
keywords: Pseudomonas syringae; hrp PAI; plant phenolic compounds; plant-pathogen interaction; qRT-PCR