title: Naringin Protects Against Cartilage Destruction in Osteoarthritis Through Repression of NF-κB Signaling Pathway.
abstract: Naringin was previously reported as a multifunctional agent. Recently, naringin was found to play a protective role in various inflammatory conditions. However, the role of naringin in cartilage degeneration and osteoarthritis (OA) progression is still unknown. TNF-α is reported to play a detrimental role in OA. Herein, primary murine chondrocytes were isolated and cultured with stimulation of TNF-α, in the presence or absence of naringin treatment. As a result, naringin attenuated TNF-α-mediated inflammation and catabolism in chondrocyte. Besides, surgically induced OA mice models were established. Cartilage degradation and OA severity were evaluated using Safranin-O staining, immunohistochemistry, and ELISA. Moreover, levels of inflammatory cytokines and catabolic markers in OA were analyzed. Oral administration of naringin alleviated degradation of cartilage matrix and protected against OA development in the surgically induced OA models. Furthermore, the protective function of naringin in cartilage and chondrocyte was possibly due to suppression of NF-κB signaling pathway. Collectively, this study presents naringin as a potential target for the treatment of joint degenerative diseases, including OA.
authors.affiliation: Department of Orthopaedics, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, Shandong, 250012, People's Republic of China. miraculously2008@163.com.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedics, Liaocheng Clinical Academy, Taishan Medical University, Liaocheng, Shandong, 252000, People's Republic of China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Pathology, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, Shandong, 250012, People's Republic of China.
authors.forename: Wenhan
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Pathology, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, Shandong, 250012, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Biopharmaceuticals of Shandong Province, Jinan, Shandong, 250101, People's Republic of China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Pathology, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, Shandong, 250012, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Su
authors.affiliation: Department of Pathology, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, Shandong, 250012, People's Republic of China.
authors.forename: Long
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Pathology, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, Shandong, 250012, People's Republic of China. liweiweizhao@163.com.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Li
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000473:pathology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D044950:Flavanones / Q000494:pharmacology*; D006614:Hindlimb / Q000473:pathology; D007249:Inflammation / Q000188:drug therapy; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy* / Q000517:prevention & control; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D016207:Cytokines; D044950:Flavanones; C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D051722:ADAM Proteins; D053509:Matrix Metalloproteinase 13; C507081:Mmp13 protein, mouse; C005274:naringin
keywords: NF-κB signaling; TNF-α; chondrocyte; naringin; osteoarthritis