title: The application of CD73 in minimal residual disease monitoring using flow cytometry in B-cell acute lymphoblastic leukemia.
abstract: The expression of CD73 by flow cytometry (FC) in bone marrow (BM) specimens of B-cell acute lymphoblastic leukemia (B-ALL) with or without minimal residual disease (MRD) was studied, and its advantages were evaluated using the MRD assay. This study also detected the expression profile of CD73 in hematogones and mature B cells in BM specimens of 18 healthy donors. Results showed that the mean value of CD73 expression in MRD-positive B cells was 6-fold greater than that in the MRD negative ones. Also, 41.82% MRD-positive B-ALL cases expressed high CD73 and the sensitivity of CD73-based MRD detection reached 10(-4). Since the expression of CD73 increases with the maturation of normal B cells, it is better to mix it with CD34, CD10 and CD20 in one tube to prevent the disturbance of mature B cells. CD73 is recommended as an optional MRD marker for B-ALL patients by using FC.
authors.affiliation: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors.forename: Zhen-Ling
authors.initials: ZL
authors.lastname: Li
authors.affiliation: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Gong
authors.affiliation: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors.forename: Fan-Zhou
authors.initials: FZ
authors.lastname: Huang
authors.affiliation: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors.forename: Yan-Rong
authors.initials: YR
authors.lastname: Chen
authors.affiliation: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Zhang
authors.affiliation: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors.forename: Ya-Yue
authors.initials: YY
authors.lastname: Gao
authors.affiliation: a Department of Hematology , China-Japan Friendship Hospital , Beijing , PR China.
authors.forename: Yi-Gai
authors.initials: YG
authors.lastname: Ma
mesh_terms: D015720:5'-Nucleotidase / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D014408:Biomarkers, Tumor; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D018365:Neoplasm, Residual / Q000175:diagnosis* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; D015720:5'-Nucleotidase
keywords: B-cell acute lymphoblastic leukemia; flow cytometry; minimal residual disease