title: Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats.
abstract: BACKGROUND
  Impaired wound healing is a complication of diabetes and a serious problem in clinical practice. We previously found that whey protein (WP) was able to regulate wound healing normally in streptozotocin (STZ)-diabetic models. This subsequent study was designed to assess the effect of WP on heat shock protein-72 (Hsp72) and keratin16 (Krt16) expression during wound healing in diabetic rats.
  
  
  METHODS
  WP at a dosage of 100 mg/kg of body weight was orally administered daily to wounded normal and STZ-diabetic rats for 8 days.
  
  
  RESULTS
  At day 4, the WP-treated diabetic wound was significantly reduced compared to that in the corresponding control. Diabetic wounded rats developed severe inflammatory infiltration and moderate capillary dilatation and regeneration. Treated rats had mild necrotic formation, moderate infiltration, moderate to severe capillary dilatation and regeneration, in addition to moderate epidermal formation. Hsp72 and Krt16 densities showed low and dense activity in diabetic wounded and diabetic wounded treated groups, respectively. At day 8, WP-treatment of diabetic wounded animals revealed great amelioration with complete recovery and closure of the wound. Reactivity of Hsp72 and Krt16 was reversed, showing dense and low, or medium and low, activity in the diabetic wounded and diabetic wounded treated groups, respectively. Hsp72 expression in the pancreas was found to show dense reactivity with WP-treated diabetic wound rats.
  
  
  CONCLUSIONS
  This data provides evidence for the potential impact of WP in the up-regulation of Hsp72 and Krt16 in T1D, resulting in an improved wound healing process in diabetic models.
authors.affiliation: Cell Biology and Histology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt. shorouk2002os@yahoo.com.
authors.forename: Rasha R
authors.initials: RR
authors.lastname: Ahmed
authors.affiliation: Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt. aymon411@yahoo.com.
authors.forename: Ayman
authors.initials: A
authors.lastname: Mahmoud
authors.affiliation: Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt. osamamoha@yahoo.com.
authors.forename: Osama M
authors.initials: OM
authors.lastname: Ahmed
authors.affiliation: Department of Food Science, College of Agriculture and Food Science, King Saud University, Riyadh, Saudi Arabia. ali_metwalli@yahoo.com.|Department of Dairy, Faculty of Agriculture, El-Minia University, El-Minia, Egypt. ali_metwalli@yahoo.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Metwalli
authors.affiliation: Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, KSA. hossamebaid@yahoo.com.|Department of Zoology, Faculty of Science, El-Minia University, El-Minia, Egypt. hossamebaid@yahoo.com.
authors.forename: Hossam
authors.initials: H
authors.lastname: Ebaid
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000178:diet therapy*; D050884:HSP72 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D053536:Keratin-16 / Q000235:genetics / Q000378:metabolism*; D007928:Lethal Dose 50; D020556:Neutrophil Infiltration / Q000187:drug effects; D010179:Pancreas / Q000378:metabolism; D051381:Rats; D012867:Skin / Q000378:metabolism; D015854:Up-Regulation; D000067816:Whey Proteins / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D050884:HSP72 Heat-Shock Proteins; D053536:Keratin-16; D000067816:Whey Proteins