title: New insights into the impact of Lactobacillus population on host-bacteria metabolic interplay.
abstract: We aimed at evaluating the association between intestinal Lactobacillus sp. composition and their metabolic activity with the host metabolism in adult and elderly individuals. Faecal and plasma metabolites were measured and correlated to the Lactobacillus species distribution in healthy Estonian cohorts of adult (n = 16; < 48 y) and elderly (n = 33; > 65 y). Total cholesterol, LDL, C-reactive protein and glycated hemoglobin were statistically higher in elderly, while platelets, white blood cells and urinary creatinine were higher in adults. Aging was associated with the presence of L. paracasei and L. plantarum and the absence of L. salivarius and L. helveticus. High levels of intestinal Lactobacillus sp. were positively associated with increased concentrations of faecal short chain fatty acids, lactate and essential amino acids. In adults, high red blood cell distribution width was positively associated with presence of L. helveticus and absence of L. ruminis. L. helveticus was correlated to lactate and butyrate in faecal waters. This indicates a strong relationship between the composition of the gut Lactobacillus sp. and host metabolism. Our results confirm that aging is associated with modulations of blood biomarkers and intestinal Lactobacillus species composition. We identified specific Lactobacillus contributions to gut metabolic environment and related those to blood biomarkers. Such associations may prove useful to decipher the biological mechanisms underlying host-gut microbial metabolic interactions in an ageing population.
authors.affiliation: Department of Food and Nutritional Sciences, The University of Reading, Whiteknights, Reading, United Kingdom.
authors.forename: Caroline I
authors.initials: CI
authors.lastname: Le Roy
authors.affiliation: Department of Microbiology, University of Tartu, Tartu, Estonia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Štšepetova
authors.affiliation: Department of Microbiology, University of Tartu, Tartu, Estonia.
authors.forename: Epp
authors.initials: E
authors.lastname: Sepp
authors.affiliation: Bio-competence Center of Healthy Dairy Production LLC, Tartu, Estonia.
authors.forename: Epp
authors.initials: E
authors.lastname: Songisepp
authors.affiliation: Department of Food and Nutritional Sciences, The University of Reading, Whiteknights, Reading, United Kingdom.
authors.forename: Sandrine P
authors.initials: SP
authors.lastname: Claus
authors.affiliation: Department of Microbiology, University of Tartu, Tartu, Estonia.
authors.forename: Marika
authors.initials: M
authors.lastname: Mikelsaar
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging; D000601:Amino Acids, Essential / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D001772:Blood Cell Count; D015992:Body Mass Index; D004734:Energy Metabolism / Q000502:physiology*; D004957:Estonia; D005227:Fatty Acids / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics
chemical_list: D000601:Amino Acids, Essential; D015415:Biomarkers; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S
keywords: Gerotarget; Lactobacillus; elderly; immunology; nuclear magnetic resonance