title: Creating Genome Modifications in C. elegans Using the CRISPR/Cas9 System.
abstract: The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe a method using the S. pyogenes Cas9 in C. elegans that provides a convenient and effective approach for making heritable changes to the worm genome.
authors.affiliation: FAS Center for Systems Biology, Harvard University, Northwest Lab Building, 52 Oxford Street, B227.80, Cambridge, MA, 02138, USA. jcalarco@fas.harvard.edu.
authors.forename: John A
authors.initials: JA
authors.lastname: Calarco
authors.affiliation: Editas Medicine, Cambridge, MA, USA.
authors.forename: Ari E
authors.initials: AE
authors.lastname: Friedland
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D064113:CRISPR-Cas Systems / Q000235:genetics*; D017173:Caenorhabditis elegans / Q000235:genetics*; D003001:Cloning, Molecular; D018390:Gene Targeting / Q000379:methods; D005818:Genetic Engineering / Q000379:methods*; D016678:Genome; D054643:INDEL Mutation; D010957:Plasmids / Q000235:genetics; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000235:genetics
chemical_list: D000094704:RNA, Guide, CRISPR-Cas Systems
keywords: C. elegans; CRISPR; Cas9; Genome editing; Genome engineering