title: Yeast Mitochondria as a Model System to Study the Biogenesis of Bacterial β-Barrel Proteins.
abstract: Beta-barrel proteins are found in the outer membrane of Gram-negative bacteria, mitochondria, and chloroplasts. The evolutionary conservation in the biogenesis of these proteins allows mitochondria to assemble bacterial β-barrel proteins in their functional form. In this chapter, we describe exemplarily how the capacity of yeast mitochondria to process the trimeric autotransporter YadA can be used to study the role of bacterial periplasmic chaperones in this process.
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tübingen, Hoppe-Seyler-Straße 4, Tübingen, 72076, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Ulrich
authors.affiliation: Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Tübingen, 72076, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Oberhettinger
authors.affiliation: Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Tübingen, 72076, Germany.
authors.forename: Ingo B
authors.initials: IB
authors.lastname: Autenrieth
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tübingen, Hoppe-Seyler-Straße 4, Tübingen, 72076, Germany. doron.rapaport@uni-tuebingen.de.
authors.forename: Doron
authors.initials: D
authors.lastname: Rapaport
mesh_terms: D018829:Adhesins, Bacterial / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D016209:Interleukin-8 / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D018829:Adhesins, Bacterial; D016209:Interleukin-8; C064832:YadA protein, Yersinia
keywords: BAM complex; Chaperones; Evolutionary conservation; Mitochondria; Skp; TOB complex; β-Barrel proteins