title: Resistance of Castanea mollissima Shuhe-WYL strain to Dryocosmus kuriphilus and its molecular mechanism.
abstract: The resistance of Castanea mollissima Shuhe-WYL strain to Dryocosmus kuriphilus and its molecular mechanism were examined. The larvae of D. kuriphilus were inoculated on the Shuhe-WYL and Qingzha strains, and mortality was observed and compared; the relative mRNA content of the OsCDPK2, receptor-like protein, OsNAC6 protein, KH domain protein, RNA-binding protein, and the bHLH genes was detected using real-time polymerase chain reaction, and then compared between the Shuhe-WYL and Qingzha strains. Phenylalanine ammonia-lyase content was detected by western blotting and compared between the inoculated Shuhe-WYL, non-inoculated Shuhe-WYL, and inoculated Qingzha strains. The mortalities of larvae inoculated on the bud, bracteal leaf, and cardiac lobe were lower in Shuhe-WYL than Qingzha at 48 and 96 h after inoculation; the contents of OsCDPK2, receptor-like protein, OsNAC6 protein, and bHLH in the cardiac lobe were higher in Shuhe-WYL than in Qingzha at 96 h after inoculation, but KH domain protein and RNA-binding protein were not significantly different. The content of phenylalanine ammonia-lyase in the cardiac lobe was higher in inoculated and non-inoculated Shuhe-WYL compared to inoculated Qingzha at 15, 30, 45, and 60 days, and higher in inoculated Shuhe-WYL than in non-inoculated Shuhe-WYL at 15, 30, 45, and 60 days. The content of phenylalanine ammonia-lyase in the cardiac lobe of inoculated Shuhe-WYL had no significant difference between at 60 and at 45 days; and was higher at 60 and 45 days than at 30 and 15 days; and was higher at 30 days than at 15 days (60≈45˃30˃15 days). The C. mollissima Shuhe-WYL strain was resistant to D. kuriphilus; high expression of OsCDPK2, receptor-like protein, OsNAC6 protein, and bHLH and phenylalanine ammonia-lyase may explain the mechanism.
authors.affiliation: Institute of Botany, Jiangsu Province and The Chinese Academy of Sciences, Nanjing, China ggmin123456@163.com.
authors.forename: G M
authors.initials: GM
authors.lastname: Geng
authors.affiliation: Institute of Botany, Jiangsu Province and The Chinese Academy of Sciences, Nanjing, China.
authors.forename: C C
authors.initials: CC
authors.lastname: Zhu
authors.affiliation: Institute of Botany, Jiangsu Province and The Chinese Academy of Sciences, Nanjing, China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhou
mesh_terms: D000818:Animals; D060467:Disease Resistance / Q000235:genetics*; D029962:Fagaceae / Q000235:genetics* / Q000276:immunology* / Q000469:parasitology; D018506:Gene Expression Regulation, Plant; D007814:Larva; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000469:parasitology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016019:Survival Analysis; D014863:Wasps / Q000502:physiology*
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D010650:Phenylalanine Ammonia-Lyase