title: Generation of a bioengineered autologous bone substitute for palate repair: an in vivo study in laboratory animals.
abstract: We carried out an in vivo study to evaluate the potential usefulness of a novel bioengineered bone substitute for the repair of palate defects in laboratory rabbits, using tissue-engineering methods. Our results showed that the use of a bioengineered bone substitute was associated with more symmetrical palate growth as compared to the controls, and the length and height of the palate were very similar on both sides of the palate, with differences from negative controls 4 months after artificial bone grafting for bone length. The histological analysis revealed that the regenerated bone was well organized and expressed osteocalcin. In contrast, bone corresponding to control animals without tissue grafting was immature, with areas of osteoid tissue and remodelling, as determined by MMP-14 expression. These results suggest that bone substitutes may be a useful strategy to induce the formation of a well-structured palate bone, which could prevent the growth alterations found in cleft palate patients. This opens a door to a future clinical application of these bone substitutes. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Division of Paediatric Surgery, University Hospital Virgen de las Nieves, Granada, Spain.|PhD programme in Biomedicine, University of Granada, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Liceras-Liceras
authors.affiliation: Tissue Engineering Group, Department of Histology, University of Granada and Instituto de Investigación Biosanitaria ibs.Granada, Spain.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Garzón
authors.affiliation: Craniofacial Malformations and Cleft Lip and Palate Management Unit, University Hospital Virgen de las Nieves, Granada, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: España-López
authors.affiliation: Tissue Engineering Group, Department of Histology, University of Granada and Instituto de Investigación Biosanitaria ibs.Granada, Spain.
authors.forename: Ana-Celeste-Ximenes
authors.initials: AC
authors.lastname: Oliveira
authors.affiliation: Division of Paediatric Surgery, University Hospital Virgen de las Nieves, Granada, Spain.
authors.forename: Miriam
authors.initials: M
authors.lastname: García-Gómez
authors.affiliation: Tissue Engineering Group, Department of Histology, University of Granada and Instituto de Investigación Biosanitaria ibs.Granada, Spain.
authors.forename: Miguel-Ángel
authors.initials: MÁ
authors.lastname: Martín-Piedra
authors.affiliation: Department of Human Anatomy and Embryology, University of Granada, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: Roda
authors.affiliation: Department of Zoology, Faculty of Sciences, University of Granada, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Alba-Tercedor
authors.affiliation: Tissue Engineering Group, Department of Histology, University of Granada and Instituto de Investigación Biosanitaria ibs.Granada, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Alaminos
authors.affiliation: Division of Paediatric Surgery, University Hospital Virgen de las Nieves, Granada, Spain.|Tissue Engineering Group, Department of Histology, University of Granada and Instituto de Investigación Biosanitaria ibs.Granada, Spain.|Craniofacial Malformations and Cleft Lip and Palate Management Unit, University Hospital Virgen de las Nieves, Granada, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Fernández-Valadés
mesh_terms: D000818:Animals; D064592:Autografts; D001861:Bone Regeneration; D018786:Bone Substitutes; D002972:Cleft Palate / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D005786:Gene Expression Regulation; D053511:Matrix Metalloproteinase 14 / Q000096:biosynthesis*; D010159:Palate / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D011817:Rabbits; D023822:Tissue Engineering
chemical_list: D018786:Bone Substitutes; C000603532:Mmp14 protein, rat; D053511:Matrix Metalloproteinase 14
keywords: biomaterials; bone; cleft palate; fibrin-agarose; stem cells; tissue engineering