title: Vitis thunbergii var. taiwaniana Extracts and Purified Compounds Ameliorate Obesity in High-Fat Diet-Induced Obese Mice.
abstract: The increasing prevalence of obesity continues to gain more attention worldwide. In this study, diet-induced obese mice were used to evaluate the antiobesity effects of extracts, fractions, and purified compounds from Vitis thunbergii var. taiwaniana (VTT). The C57BL/6J mice were fed a 5-week high-fat diet (HF) concurrently with ethanol extracts (Et-ext, 80 mg/kg) from roots (R), stems (S), and leaves (L) by oral gavage daily. Only R-Et-ext interventions showed significant weight reduction in mice compared with those in the HF group; however, mouse plasma contents of total cholesterols (TC), total triglycerides (TG) and low-density lipoproteins (LDL) of all three Et-ext intervened groups showed significant reductions compared with those in the HF group. Furthermore, intervention with the ethyl acetate-partitioned fraction (EA-fra, 60 mg/kg) from R-Et-ext but not the n-butanol-partitioned fraction or water fraction from R-Et-ext showed significant weight reduction in mice compared with those in the HF group. The same molecular weights of three resveratrol tetramers, (+)-hopeaphenol, (+)-vitisin A, and (-)-vitisin B, were isolated from the EA-fra of VTT-R. The (+)-vitisin A and fenofibrate (25 mg/kg) but not the (+)-hopeaphenol and (-)-vitisin B interventions showed significant weight reduction in mice compared with those in the HF group. The total feed intake among the HF groups with or without interventions showed no significant differences. The mouse plasma contents of TC, TG, LDL, free fatty acid, and plasma lipase activity of the three resveratrol tetramer-intervened groups showed reductions in the mice compared with those in the HF group. It was proposed that the lipase inhibitory activities of VTT extracts and purified resveratrol tetramers might contribute in part to the antiobesity effect, and these results suggested that VTT may be developed as functional food for achieving antiobesity objectives and requires further investigation.
authors.affiliation: Department of General Medicine, Taipei Medical University , and Department of Primary Care Medicine, Taipei Medical University Hospital, Taipei 110, Taiwan.
authors.forename: Shyr-Yi
authors.initials: SY
authors.lastname: Lin
authors.affiliation: Division of Hemato-oncology, Department of Internal Medicine, Yuan's General Hospital , Kaohsiung 80249, Taiwan.|Department of Leisure and Recreation Industry Management, College of Humanities and Management, Fooyin University , Kaohsiung 831, Taiwan.|School of Medicine, Taipei Medical University , Taipei 110, Taiwan.
authors.forename: Guan-Cheng
authors.initials: GC
authors.lastname: Huang
authors.affiliation: Graduate Institute of Pharmacognosy, Taipei Medical University , Taipei 110, Taiwan.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Hsieh
authors.affiliation: Graduate Institute of Pharmacognosy, Taipei Medical University , Taipei 110, Taiwan.
authors.forename: Yin-Shiou
authors.initials: YS
authors.lastname: Lin
authors.affiliation: Department of Health and Creative Vegetarian Science, Fo Guang University , Yilan 262, Taiwan.
authors.forename: Chuan-Hsiao
authors.initials: CH
authors.lastname: Han
authors.affiliation: Taiwan Seed Improvement and Propagation Station, Council of Agriculture , Taichung 426, Taiwan.|Traditional Herbal Medicine Research Center, Taipei Medical University Hospital , Taipei 110, Taiwan.
authors.forename: Chi-Luan
authors.initials: CL
authors.lastname: Wen
authors.affiliation: Department of Biological Science and Technology, National Pingtung University of Science and Technology , Pingtung 912, Taiwan.
authors.forename: Chi-I
authors.initials: CI
authors.lastname: Chang
authors.affiliation: Graduate Institute of Pharmacognosy, Taipei Medical University , Taipei 110, Taiwan.|Traditional Herbal Medicine Research Center, Taipei Medical University Hospital , Taipei 110, Taiwan.
authors.forename: Wen-Chi
authors.initials: WC
authors.lastname: Hou
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage; D013624:Taiwan; D014280:Triglycerides / Q000378:metabolism; D027843:Vitis / Q000737:chemistry*
chemical_list: D008077:Lipoproteins, LDL; D010936:Plant Extracts; D013267:Stilbenes; D014280:Triglycerides; D002784:Cholesterol; D000077185:Resveratrol
keywords: (+)-vitisin A; Vitis thunbergii var. taiwaniana (VTT); high-fat diet (HF); lipase; obesity