title: Efficacy of combined vaccination against Mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus in dually infected pigs.
abstract: Porcine respiratory disease complex (PRDC) is one of the main causes of economic losses for swine producers. This complex is due to a combination of different pathogens and their interactions. Two major pathogens involved in PRDC are Mycoplasma hyopneumoniae (Mhp) and porcine reproductive and respiratory syndrome virus (PRRSV). The objectives of this study were (i) to develop an experimental model of dual Mhp/PRRSV infection in SPF pigs with European strains of Mhp and PRRSV and (ii) to assess and compare the effects of single Mhp, single PRRSV or combined Mhp/PRRSV vaccination against this dual infection. Pigs dually infected with Mhp and PRRSV showed a combination of symptoms characteristic of each pathogen but no significant exacerbation of pathogenicity. Thus, the co-infected pigs displayed coughing and pneumonia typical of Mhp infection in addition to PRRSV-related hyperthermia and decrease in average daily gain (ADG). Hyperthermia was reduced in PRRSV vaccinated animals (single or combined vaccination), whereas ADG was restored in Mhp/PRRSV vaccinated pigs only. Regarding respiratory symptoms and lung lesions, no vaccine decreased coughing. However, all vaccines reduced the pneumonia score but more so in animals receiving the Mhp vaccine, whether single or combined. This vaccine also decreased the Mhp load in the respiratory tract. In conclusion, combined vaccination against both Mhp and PRRSV efficiently pooled the efficacy of each single PRRSV and Mhp vaccination and could be an interesting tool to control PRDC in European swine production.
authors.affiliation: French Agency for Food, Environmental and Occupational Health & Safety (Anses-Agence Nationale de Sécurité Sanitaire Alimentation Environnement Travail), Unité Virologie Immunologie Porcines, BP 53, 22440 Ploufragan, France; European University of Brittany, France. Electronic address: olivier.bourry@anses.fr.
authors.forename: Olivier
authors.initials: O
authors.lastname: Bourry
authors.affiliation: Anses, Unité Epidémiologie et Bien-être Porcins, BP 53, 22440 Ploufragan, France; European University of Brittany, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Fablet
authors.affiliation: French Agency for Food, Environmental and Occupational Health & Safety (Anses-Agence Nationale de Sécurité Sanitaire Alimentation Environnement Travail), Unité Virologie Immunologie Porcines, BP 53, 22440 Ploufragan, France; European University of Brittany, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Simon
authors.affiliation: Anses, Unité Mycoplasmologie-Bactériologie, BP 53, 22440 Ploufragan, France; European University of Brittany, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Marois-Créhan
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D060085:Coinfection / Q000382:microbiology / Q000662:veterinary / Q000821:virology; D045705:Mycoplasma hyopneumoniae; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology / Q000517:prevention & control*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus; D034421:Sus scrofa; D013552:Swine; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000627:therapeutic use*
chemical_list: D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D001428:Bacterial Vaccines; D014765:Viral Vaccines
keywords: Dual infection; European strain; Mycoplasma hyopneumoniae; PRDC; PRRS; Vaccine