title: Identification, classification, and expression profiles of heat shock transcription factors in tea plant (Camellia sinensis) under temperature stress.
abstract: In vascular plants, heat shock transcription factors (Hsfs) regulate heat stress response by regulating the expression of heat shock proteins. This study systematically and comprehensively analyzed the Hsf family in tea plant [Camellia sinensis (L.) O. Kuntze]. A total of 16 CsHsfs were identified from the transcriptome database of tea plant and analyzed for their phylogenetic relationships, motifs, and physicochemical characteristics. On the basis of the phylogenetic comparison of tea plant with Arabidopsis thaliana, Populus trichocarpa, Theobroma cacao, and Oryza sativa, the CsHsfs were classified into three classes, namely, A (56.25%), B (37.50%), and C (6.25%). Heat mapping showed that the expression profiles of CsHsf genes under non-stress conditions varied among four tea plant cultivars, namely, 'Yunnanshilixiang', 'Chawansanhao', 'Ruchengmaoyecha', and 'Anjibaicha'. Six CsHsf genes (CsHsfA1a, CsHsfA1b, CsHsfA6, CsHsfB1, CsHsfB2b, and CsHsfC1) were selected from classes A, B, and C to analyze the expression profiles of CsHsf genes through quantitative real-time PCR in 'Yingshuang', 'Anjibaicha', and 'Yunnanshilixiang' under high (38 °C) or low (4 °C) temperature stress. Temperature stress positively or negatively regulated all of the selected CsHsf genes, and the expression levels evidently varied even among CsHsf genes belonging to the same class. This study provided a relatively detailed summary of Hsfs in tea plant and may serve as a reference for further studies on the mechanism of temperature stress regulation by CsHsfs.
authors.affiliation: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Liu
authors.affiliation: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Wu
authors.affiliation: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xing-Hui
authors.initials: XH
authors.lastname: Li
authors.affiliation: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: zhuangjing@njau.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhuang
mesh_terms: D028241:Camellia sinensis / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D000076249:Heat Shock Transcription Factors; D018869:Heat-Shock Response / Q000502:physiology*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; D010940:Plant Proteins; D014157:Transcription Factors
keywords: Hsf; Quantitative real-time PCR; Tea plant; Temperature stresses; Transcription factors