title: Identification of novel microtubule-binding proteins by taxol-mediated microtubule stabilization and mass spectrometry analysis.
abstract: Microtubule-binding proteins (MBPs) are structurally and functionally diverse regulators of microtubule-mediated cellular processes. Alteration of MBPs has been implicated in the pathogenesis of human diseases, including cancer. MBPs can stabilize or destabilize microtubules or move along microtubules to transport various cargoes. In addition, MBPs can control microtubule dynamics through direct interaction with microtubules or coordination with other proteins. To better understand microtubule structure and function, it is necessary to identify additional MBPs. In this study, we isolated microtubules and MBPs from mammalian cells by a taxol-based method and then profiled a panel of MBPs by mass spectrometry. We discovered a number of previously uncharacterized MBPs, including several membrane-associated proteins and proteins involved in post-translational modifications, in addition to several structural components. These results support the notion that microtubules have a wide range of functions and may undergo more exquisite regulation than previously recognized.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Xianfei
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Biochemistry, School of Basic Medical Sciences, Tianjin Medical University Tianjin, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: He
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Dengwen
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University Tianjin, China ; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences Tianjin, China.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Shui
authors.affiliation: Department of Biochemistry, School of Basic Medical Sciences, Tianjin Medical University Tianjin, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
keywords: Mass spectrometry; microtubule; microtubule-binding protein; taxol; tubulin