title: Unraveling systematic inventory of Echinops (Asteraceae) with special reference to nrDNA ITS sequence-based molecular typing of Echinops abuzinadianus.
abstract: The present study explored the systematic inventory of Echinops L. (Asteraceae) of Saudi Arabia, with special reference to the molecular typing of Echinops abuzinadianus Chaudhary, an endemic species to Saudi Arabia, based on the internal transcribed spacer (ITS) sequences (ITS1-5.8S-ITS2) of nuclear ribosomal DNA. A sequence similarity search using BLAST and a phylogenetic analysis of the ITS sequence of E. abuzinadianus revealed a high level of sequence similarity with E. glaberrimus DC. (section Ritropsis). The novel primary sequence and the secondary structure of ITS2 of E. abuzinadianus could potentially be used for molecular genotyping.
authors.affiliation: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia alimohammad@ksu.edu.sa.
authors.forename: M A
authors.initials: MA
authors.lastname: Ali
authors.affiliation: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: F M
authors.initials: FM
authors.lastname: Al-Hemaid
authors.affiliation: Department of Environment and Forest Resources, Chungnam National University, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: A A
authors.initials: AA
authors.lastname: Hatamleh
authors.affiliation: Institute of Genetics and Biotechnology, St. István University, Gödöllo, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Gyulai
authors.affiliation: Department of Botany, University of Dhaka, Dhaka, Bangladesh.
authors.forename: M O
authors.initials: MO
authors.lastname: Rahman
mesh_terms: D019659:Asteraceae / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D058889:Molecular Typing / Q000379:methods; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012529:Saudi Arabia; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D021903:DNA, Ribosomal Spacer