title: Mutagenesis in Newts: Protocol for Iberian Ribbed Newts.
abstract: Newts have the remarkable capability of organ/tissue regeneration, and have been used as a unique experimental model for regenerative biology. The Iberian ribbed newt (Pleurodeles waltl) is suitable as a model animal. We have established methods for artificial insemination and efficient transgenesis using P. waltl newts. In addition to the transgenic technique, development of TALENs enables targeting mutagenesis in the newts. We have reported that TALENs efficiently disrupted targeted genes in newt embryos. In this chapter, we introduce a protocol for TALEN-mediated gene targeting in Iberian ribbed newts.
authors.affiliation: School of Life Sciences, Faculty of Medicine, Tottori University, 86 Nishimachi, Yonago, Tottori, Japan. toshih2@med.tottori-u.ac.jp.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: School of Life Sciences, Faculty of Medicine, Tottori University, 86 Nishimachi, Yonago, Tottori, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004268:DNA-Binding Proteins / Q000235:genetics; D018014:Gene Transfer Techniques; D007315:Insemination, Artificial / Q000235:genetics; D016296:Mutagenesis; D012038:Regeneration / Q000235:genetics*; D012455:Salamandridae / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D015534:Trans-Activators
keywords: Animal model; Genome editing; Molecular genetics; Newts; Regeneration; Transgenesis