title: A novel UHPLC method for the rapid and simultaneous determination of daidzein, genistein and equol in human urine.
abstract: This work reports on a novel method involving reverse-phased ultra-high performance liquid chromatography (UHPLC) plus a spectrophotometric photodiode array/fluorescence (FLR) detection system for determining the concentration of equol and major soy isoflavones (daidzein and genistein) in human urine. The proposed method was validated in terms of its linearity, sensitivity, accuracy (recovery) and precision (intra- and inter-day repeatability). The isoflavone profiles of urine samples from a group of menopausal women following oral soy isoflavone supplementation were determined and compared. Screening for equol-producer status was accomplished with high sensitivity (detection limit of the FLR detector 2.93nM). The method involves a short chromatographic run time compared to conventional HPLC methods while allowing for the simultaneous and reliable quantification of daidzein, genistein and equol in human urine. It also allows for the rapid screening of multiple urine samples when testing for equol production status and checking patient adherence to isoflavone treatment regimens.
authors.affiliation: Servicios Científico-Técnicos Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares s/n, 33300-Villaviciosa, Asturias, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Redruello
authors.affiliation: Departamento de Microbiología y Bioquímica, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares s/n, 33300-Villaviciosa, Asturias, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Guadamuro
authors.affiliation: Servicios Científico-Técnicos Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares s/n, 33300-Villaviciosa, Asturias, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Cuesta
authors.affiliation: Servicios Científico-Técnicos Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares s/n, 33300-Villaviciosa, Asturias, Spain.
authors.forename: Jorge R
authors.initials: JR
authors.lastname: Álvarez-Buylla
authors.affiliation: Departamento de Microbiología y Bioquímica, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares s/n, 33300-Villaviciosa, Asturias, Spain.
authors.forename: Baltasar
authors.initials: B
authors.lastname: Mayo
authors.affiliation: Departamento de Microbiología y Bioquímica, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares s/n, 33300-Villaviciosa, Asturias, Spain. Electronic address: sdelgado@ipla.csic.es.
authors.forename: Susana
authors.initials: S
authors.lastname: Delgado
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements; D060754:Equol / Q000652:urine*; D019833:Genistein / Q000652:urine*; D006801:Humans; D007529:Isoflavones / Q000652:urine*; D057230:Limit of Detection; D016014:Linear Models; D008593:Menopause; D010936:Plant Extracts; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D013025:Glycine max
chemical_list: D007529:Isoflavones; D010936:Plant Extracts; D060754:Equol; C004742:daidzein; D019833:Genistein
keywords: Equol; Human urine; Menopausal women; Solid phase extraction; Soy isoflavones; UHPLC