title: Reversed-phase chromatography in extended-nano space for the separation of amino acids.
abstract: In this work we used reversed-phase chromatography in extended-nano channels to separate amino acids. A hydrophobic surface modification of extended-nano channels was established. A sample mixture of fluorescein and sulforhodamine B (0.5 and 0.05mM respectively) was used for the demonstration of a reversed-phase separation mode. A small amount of sample band (30fL) was injected into the separation channel, and two compounds were successfully separated. The maximum theoretical plate number of sulforhodamine B was 300,000plates/m. Two sets of 3 amino acids (3.75mM each) were separated using 0.01M citrate buffer (pH 5.5) with 0.01M sodium perchlorate and 12 and 25% of acetonitrile as a mobile phase. A successful separation (320,000plates/m with plate height of 3.2μm for serine) was accomplished.
authors.affiliation: The University of Tokyo, Department of Applied Chemistry, 7-3-1, Hongo, Bunkyo, Tokyo 113-8656, Japan.
authors.forename: Adelina
authors.initials: A
authors.lastname: Smirnova
authors.affiliation: The University of Tokyo, Department of Applied Chemistry, 7-3-1, Hongo, Bunkyo, Tokyo 113-8656, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Shimizu
authors.affiliation: The University of Tokyo, Department of Applied Chemistry, 7-3-1, Hongo, Bunkyo, Tokyo 113-8656, Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Mawatari
authors.affiliation: The University of Tokyo, Department of Applied Chemistry, 7-3-1, Hongo, Bunkyo, Tokyo 113-8656, Japan. Electronic address: kitamori@icl.t.u-tokyo.ac.jp.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Kitamori
mesh_terms: D000596:Amino Acids / Q000302:isolation & purification*; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D019793:Fluorescein / Q000302:isolation & purification; D057927:Hydrophobic and Hydrophilic Interactions; D012235:Rhodamines / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D002021:Buffers; D012235:Rhodamines; C022027:lissamine rhodamine B; D019793:Fluorescein
keywords: Chip-based separation; Extended nanospace; Liquid chromatography; Nanofluidics; Reversed phase chromatography