title: Bovine mastitis may be associated with the deprivation of gut Lactobacillus.
abstract: Bovine mastitis is an economical important microbial disease in dairy industry. Some recent human clinical trials have shown that oral probiotics supplementation could effectively control clinical mastitis, suggesting that the mechanism of mastitis protection might be achieved via the host gut microbiota. We aimed to test our hypothesis that bovine mastitis was related to changes in both the mammary and gut microbial profiles. By quantitative PCR, the milk and faecal microbial profiles of cows with low (<3×105cells/ml) and high (>1×106cells/ml) somatic cell count (SCC) were compared. Firstly, we observed drastic differences in both the milk and faecal microbial compositions at genus and Lactobacillus-species levels between the two groups. Secondly, the pattern of faecal microbial community changes of mastitis cows was similar to that of the milk, characterised by a general increase in the mastitis pathogens (Enterococcus, Streptococcus and Staphylococcus) and deprivation of Lactobacillus and its members (L. salivarius, L. sakei, L. ruminis, L. delbrueckii, L. buchneri, and L. acidophilus). Thirdly, only the faecal lactobacilli, but not bifidobacteria correlated with the milk microbial communities and SCC. Our data together hint to a close association between bovine mastitis, the host gut and milk microbiota.
authors.affiliation: 1 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education P.R. China, Inner Mongolia Agricultural University, Inner Mongolia, Hohhot 010018, China P.R.
authors.forename: C
authors.initials: C
authors.lastname: Ma
authors.affiliation: 1 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education P.R. China, Inner Mongolia Agricultural University, Inner Mongolia, Hohhot 010018, China P.R.
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.affiliation: 1 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education P.R. China, Inner Mongolia Agricultural University, Inner Mongolia, Hohhot 010018, China P.R.
authors.forename: X
authors.initials: X
authors.lastname: Xi
authors.affiliation: 1 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education P.R. China, Inner Mongolia Agricultural University, Inner Mongolia, Hohhot 010018, China P.R.
authors.forename: J
authors.initials: J
authors.lastname: Ding
authors.affiliation: 1 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education P.R. China, Inner Mongolia Agricultural University, Inner Mongolia, Hohhot 010018, China P.R.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: 1 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education P.R. China, Inner Mongolia Agricultural University, Inner Mongolia, Hohhot 010018, China P.R.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.affiliation: 1 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education P.R. China, Inner Mongolia Agricultural University, Inner Mongolia, Hohhot 010018, China P.R.
authors.forename: L Y
authors.initials: LY
authors.lastname: Kwok
mesh_terms: D000818:Animals; D002417:Cattle; D005243:Feces / Q000382:microbiology; D005260:Female; D000069196:Gastrointestinal Microbiome; D007778:Lactobacillus; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000382:microbiology
keywords: Bifidobacterium; Lactobacillus; gastrointestinal tract; gut microbiota; mammary infection; mastitis