title: Enhanced expression of TWIK-related arachidonic acid-activated K+ channel in the spinal cord of detrusor overactivity rats after partial bladder outlet obstruction.
abstract: BACKGROUND
  Detrusor overactivity (DO) secondary to partial bladder outlet obstruction (PBOO) is closely associated with alteration of ion channels. The objective of this study is to investigate the expression of the TWIK-related arachidonic acid-activated K(+) channel (TRAAK) in the L6-S1 spinal cord of DO rats after PBOO.
  
  
  METHODS
  Female Sprague-Dawley rats undergoing PBOO surgery were screened for DO by cystometry. Sham-operated rats served as controls. The expression of TRAAK in the L6-S1 spinal cord was detected by real-time polymerase chain reaction, western blotting and immunohistochemistry.
  
  
  RESULTS
  DO was successfully induced after chronic PBOO in rats, with an incidence rate of 62.5 %. Compared with sham-operated rats, the expression of TRAAK in the L6-S1 spinal cord of DO rats was significantly increased at the mRNA (1.886 ± 0.710 versus 0.790 ± 0.679, P < 0.05) and protein level (0.510 ± 0.087 versus 0.255 ± 0.107, P < 0.05). Immunohistochemical staining showed increased expression of TRAAK in the dorsal horn and ventral horn of the spinal cord.
  
  
  CONCLUSIONS
  Upregulation of TRAAK was observed in the spinal cord of DO rats after chronic PBOO, which may exert a protective effect against DO by suppressing the excitability of neurons.
authors.affiliation: Department of Urology, The First Affiliated Hospital of Sun Yat-Sen University, NO. 58 Zhongshan Er Road, Guangzhou, 510080, China. zhangjunlong1985@163.com.
authors.forename: Junlong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Urology, The First Affiliated Hospital of Sun Yat-Sen University, NO. 58 Zhongshan Er Road, Guangzhou, 510080, China. shutongxuan@126.com.
authors.forename: Mingxin
authors.initials: M
authors.lastname: Cao
authors.affiliation: Department of Urology, HuiZhou Affiliated Hospital, Sun Yat-sen University (HuiZhou Municipal Central Hospital), Huizhou, Guangdong, China. wxl21@139.com.
authors.forename: Xilian
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Urology, The Eastern Hospital of the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China. hugochan68@hotmail.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Urology, The First Affiliated Hospital of Sun Yat-Sen University, NO. 58 Zhongshan Er Road, Guangzhou, 510080, China. lwj5447@163.com.
authors.forename: Weijie
authors.initials: W
authors.lastname: Liang
authors.affiliation: Department of Urology, The First Affiliated Hospital of Sun Yat-Sen University, NO. 58 Zhongshan Er Road, Guangzhou, 510080, China. uroliang@126.com.
authors.forename: Yueyou
authors.initials: Y
authors.lastname: Liang
mesh_terms: D000818:Animals; D005260:Female; D015640:Ion Channel Gating; D015221:Potassium Channels / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism*; D015854:Up-Regulation; D001748:Urinary Bladder Neck Obstruction / Q000150:complications / Q000378:metabolism*; D053201:Urinary Bladder, Overactive / Q000209:etiology / Q000378:metabolism*
chemical_list: C503434:Kcnk4 protein, rat; D015221:Potassium Channels