title: Halorubrum rutilum sp. nov. isolated from a marine solar saltern.
abstract: A halophilic archaeal strain, YJ-18-S1(T), was isolated from Yangjiang marine solar saltern, Guangxi Province, China. Cells were pleomorphic, stained Gram-negative and formed red-pigmented colonies on agar plates. Strain YJ-18-S1(T) was able to grow at 20-55 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 2.6 M NaCl), at 0.005-1.0 M MgCl2 (optimum 0.3 MgCl2) and at pH 5.5-8.5 (optimum pH 7.0). The cells were lysed in distilled water, and the minimal NaCl concentration to prevent cell lysis was found to be 5 % (w/v). The major polar lipids of the strain were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and sulfated mannosyl glucosyl diether. The 16S rRNA gene and rpoB' gene of strain YJ-18-S1(T) were phylogenetically related to the corresponding genes of Halorubrum members (94.3-98.0 and 86.7-96.1 % similarities, respectively). The DNA G+C content of strain YJ-18-S1(T) was 66.2 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YJ-18-S1(T) (=CGMCC 1.12554(T) = JCM 30030(T)) represents a new species of Halorubrum, for which the name Halorubrum rutilum sp. nov. is proposed.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Yin
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
authors.forename: Jia-Qi
authors.initials: JQ
authors.lastname: Xu
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
authors.forename: Wen-Mei
authors.initials: WM
authors.lastname: Xu
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Yuan
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China. cuihenglin@ujs.edu.cn.
authors.forename: Heng-Lin
authors.initials: HL
authors.lastname: Cui
mesh_terms: D001482:Base Composition / Q000235:genetics; D002681:China; D019641:DNA, Archaeal / Q000235:genetics; D055352:Halorubrum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D010743:Phospholipids / Q000235:genetics*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism*
chemical_list: D019641:DNA, Archaeal; C407124:PGP-Me; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride
keywords: Halophilic archaeon; Halorubrum rutilum sp. nov.; Marine solar saltern