title: Candidate Gene Identification with SNP Marker-Based Fine Mapping of Anthracnose Resistance Gene Co-4 in Common Bean.
abstract: Anthracnose, caused by Colletotrichum lindemuthianum, is an important fungal disease of common bean (Phaseolus vulgaris). Alleles at the Co-4 locus confer resistance to a number of races of C. lindemuthianum. A population of 94 F4:5 recombinant inbred lines of a cross between resistant black bean genotype B09197 and susceptible navy bean cultivar Nautica was used to identify markers associated with resistance in bean chromosome 8 (Pv08) where Co-4 is localized. Three SCAR markers with known linkage to Co-4 and a panel of single nucleotide markers were used for genotyping. A refined physical region on Pv08 with significant association with anthracnose resistance identified by markers was used in BLAST searches with the genomic sequence of common bean accession G19833. Thirty two unique annotated candidate genes were identified that spanned a physical region of 936.46 kb. A majority of the annotated genes identified had functional similarity to leucine rich repeats/receptor like kinase domains. Three annotated genes had similarity to 1, 3-β-glucanase domains. There were sequence similarities between some of the annotated genes found in the study and the genes associated with phosphoinositide-specific phosphilipases C associated with Co-x and the COK-4 loci found in previous studies. It is possible that the Co-4 locus is structured as a group of genes with functional domains dominated by protein tyrosine kinase along with leucine rich repeats/nucleotide binding site, phosphilipases C as well as β-glucanases.
authors.affiliation: Department of Plant Agriculture, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Burt
authors.affiliation: Integrated Breeding Platform, Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Carretera México-Veracruz, Km. 45, El Batán, Texcoco, Mexico 56237.
authors.forename: H Manilal
authors.initials: HM
authors.lastname: William
authors.affiliation: Department of Plant Agriculture, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: Gregory
authors.initials: G
authors.lastname: Perry
authors.affiliation: Department of Plant Agriculture, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: Raja
authors.initials: R
authors.lastname: Khanal
authors.affiliation: Department of Plant Agriculture, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: K Peter
authors.initials: KP
authors.lastname: Pauls
authors.affiliation: Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, Michigan 48824, United States of America.
authors.forename: James D
authors.initials: JD
authors.lastname: Kelly
authors.affiliation: Agriculture and Agri-Food Canada, Greenhouse and Processing Crops Research Centre, Harrow, Ontario, N0R 1G0, Canada.
authors.forename: Alireza
authors.initials: A
authors.lastname: Navabi
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D056726:Genetic Association Studies; D008040:Genetic Linkage; D005819:Genetic Markers; D060005:Genotyping Techniques; D006026:Glycoside Hydrolases / Q000235:genetics; D027805:Phaseolus / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D016415:Sequence Alignment; D017385:Sequence Homology; D013045:Species Specificity; D010738:Type C Phospholipases / Q000235:genetics
chemical_list: D002352:Carrier Proteins; D005819:Genetic Markers; D010940:Plant Proteins; D011505:Protein-Tyrosine Kinases; D010738:Type C Phospholipases; D006026:Glycoside Hydrolases