title: The effects of repetitive vibration on sensorineural function: biomarkers of sensorineural injury in an animal model of metabolic syndrome.
abstract: Exposure to hand-transmitted vibration in the work-place can result in the loss of sensation and pain in workers. These effects may be exacerbated by pre-existing conditions such as diabetes or the presence of primary Raynaud's phenomena. The goal of these studies was to use an established model of vibration-induced injury in Zucker rats. Lean Zucker rats have a normal metabolic profile, while obese Zucker rats display symptoms of metabolic disorder or Type II diabetes. This study examined the effects of vibration in obese and lean rats. Zucker rats were exposed to 4h of vibration for 10 consecutive days at a frequency of 125 Hz and acceleration of 49 m/s(2) for 10 consecutive days. Sensory function was checked using transcutaneous electrical stimulation on days 1, 5 and 9 of the exposure. Once the study was complete the ventral tail nerves, dorsal root ganglia and spinal cord were dissected, and levels of various transcripts involved in sensorineural dysfunction were measured. Sensorineural dysfunction was assessed using transcutaneous electrical stimulation. Obese Zucker rats displayed very few changes in sensorineural function. However they did display significant changes in transcript levels for factors involved in synapse formation, peripheral nerve remodeling, and inflammation. The changes in transcript levels suggested that obese Zucker rats had some level of sensory nerve injury prior to exposure, and that exposure to vibration activated pathways involved in injury and re-innervation.
authors.affiliation: National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505, USA.
authors.forename: Megan
authors.initials: M
authors.lastname: Kiedrowski
authors.affiliation: National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505, USA.
authors.forename: Stacey
authors.initials: S
authors.lastname: Waugh
authors.affiliation: National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505, USA.
authors.forename: Roger
authors.initials: R
authors.lastname: Miller
authors.affiliation: National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505, USA.
authors.forename: Claud
authors.initials: C
authors.lastname: Johnson
authors.affiliation: National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505, USA. Electronic address: ksk1@cdc.gov.
authors.forename: Kristine
authors.initials: K
authors.lastname: Krajnak
mesh_terms: D000818:Animals; D001703:Biophysics; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D006930:Hyperalgesia / Q000503:physiopathology*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D008297:Male; D024821:Metabolic Syndrome / Q000150:complications*; D009437:Neuralgia / Q000209:etiology*; D052248:Nitric Oxide Synthase Type I / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger; D051381:Rats; D011924:Rats, Zucker; D018341:Receptors, Adrenergic, alpha-2 / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D004561:Transcutaneous Electric Nerve Stimulation / Q000009:adverse effects; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism; D014732:Vibration / Q000009:adverse effects*
chemical_list: C547770:Adra2a protein, rat; D018932:Chemokine CCL2; D053583:Interleukin-1beta; D012333:RNA, Messenger; D018341:Receptors, Adrenergic, alpha-2; C002744:3-nitrotyrosine; D014443:Tyrosine; D052248:Nitric Oxide Synthase Type I
keywords: Hyperalgesia; Metabolic disorder; Neuropathic pain; Zucker rat