title: Molecular cloning and characterization of a Spirometra erinacei casein kinase I.
abstract: The Spirometra erinacei casein kinase I (SeCKI) gene was cloned and expressed in Escherichia coli, and its characteristics were investigated in this study. The recombinant SeCP protein (rSeCKI) was purified. The vaccination of mice with rSeCKI induced the Th1/Th2-mixed type of immune response with Th2 predominant (high levels of IgG1). Western blotting analysis showed that rSeCP was recognized by the sera of plerocercoid-infected mice, and anti-rSeCP serum recognized the native SeCP protein of plerocercoid crude antigens. Transcription and expression of SeCP was observed at the plerocercoid and adult stages of S. erinacei. Immunolocalization identified SeCKI in the tegument and parenchymal tissues of plerocercoids and in the teguments of adults. SeCKI appeared to be essential indispensable for the S. erinacei development and survival in host, but its biological functions need to be further investigated.
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, People's Republic of China.
authors.forename: Li Na
authors.initials: LN
authors.lastname: Liu
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, People's Republic of China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, People's Republic of China.
authors.forename: Man
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, People's Republic of China.
authors.forename: Ming Guo
authors.initials: MG
authors.lastname: Xu
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, People's Republic of China.
authors.forename: Li Fu
authors.initials: LF
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, People's Republic of China. cuij@zzu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, People's Republic of China. wangzq@zzu.edu.cn.
authors.forename: Zhong Quan
authors.initials: ZQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D047389:Casein Kinase I / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D002590:Cestode Infections / Q000276:immunology / Q000469:parasitology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015801:Helminth Proteins / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013146:Spirometra / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000276:immunology
chemical_list: D015801:Helminth Proteins; D011994:Recombinant Proteins; D047389:Casein Kinase I
keywords: Casein kinase I (CKI); Plerocercoid; Sparganum; Spirometra erinacei