title: Decellularized Extracellular Matrix Scaffolds for Cartilage Regeneration.
abstract: Decellularized extracellular matrix (ECM) is gaining a lot of attention as a biomaterial for tissue engineering applications. This chapter describes the processing techniques for decellularization of cell-derived ECM and protocols for the fabrication of ECM-based scaffolds in the form of hydrogels or fibrous polymer meshes by electrospinning. It describes the protocols to analyze the morphology and presence of collagen in fabricated scaffolds using scanning electron microscope and Picrosirius Red staining respectively. Methods to evaluate the metabolic activity and proliferation of cells (resazurin-based assay and DNA assay, respectively) and gene expression are also presented. Furthermore, histological techniques to analyze the presence of sulfated glycosaminoglycans are also described.
authors.affiliation: Department of Biomedical Engineering, Eindhoven University of Technology, 513, 5600MB, Eindhoven, The Netherlands. s.h.thakkar@tue.nl.
authors.forename: Shraddha
authors.initials: S
authors.lastname: Thakkar
authors.affiliation: Tissue Regeneration Department, University of Maastricht - MERLN Institute for Technology Inspired Regenerative Medicine Complex, Tissue Regeneration Department Universiteitsingel 40, 6229 ER, Maastricht, The Netherlands. H.A.M.Fernandes@utwente.nl.
authors.forename: Hugo
authors.initials: H
authors.lastname: Fernandes
authors.affiliation: Tissue Regeneration Department, University of Maastricht - MERLN Institute for Technology Inspired Regenerative Medicine Complex, Tissue Regeneration Department Universiteitsingel 40, 6229 ER, Maastricht, The Netherlands. l.moroni@utwente.nl.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Moroni
mesh_terms: D062088:Acellular Dermis; D001391:Azo Compounds; D002358:Cartilage, Articular / Q000166:cytology* / Q000378:metabolism / Q000601:surgery; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D020219:Chondrogenesis; D003094:Collagen / Q000378:metabolism; D004396:Coloring Agents; D005109:Extracellular Matrix / Q000502:physiology* / Q000637:transplantation / Q000648:ultrastructure; D005786:Gene Expression Regulation; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D020100:Hydrogels; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D012038:Regeneration; D044968:Regenerative Medicine / Q000379:methods*; D013194:Staining and Labeling; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D006025:Glycosaminoglycans; D020100:Hydrogels; C009798:C.I. direct red 80; C013786:A73025; D003094:Collagen
keywords: Chondrocytes; Decellularization; Electrospinning; Extracellular matrix; Gene expression; Hydrogel; Lyophilization; Mesenchymal stromal cells