title: Antimicrobial activity of iron oxide nanoparticle upon modulation of nanoparticle-bacteria interface.
abstract: Investigating the interaction patterns at nano-bio interface is a key challenge for safe use of nanoparticles (NPs) to any biological system. The study intends to explore the role of interaction pattern at the iron oxide nanoparticle (IONP)-bacteria interface affecting antimicrobial propensity of IONP. To this end, IONP with magnetite like atomic arrangement and negative surface potential (n-IONP) was synthesized by co-precipitation method. Positively charged chitosan molecule coating was used to reverse the surface potential of n-IONP, i.e. positive surface potential IONP (p-IONP). The comparative data from fourier transform infrared spectroscope, XRD, and zeta potential analyzer indicated the successful coating of IONP surface with chitosan molecule. Additionally, the nanocrystals obtained were found to have spherical size with 10-20 nm diameter. The BacLight fluorescence assay, bacterial growth kinetic and colony forming unit studies indicated that n-IONP (<50 μM) has insignificant antimicrobial activity against Bacillus subtilis and Escherichia coli. However, coating with chitosan molecule resulted significant increase in antimicrobial propensity of IONP. Additionally, the assay to study reactive oxygen species (ROS) indicated relatively higher ROS production upon p-IONP treatment of the bacteria. The data, altogether, indicated that the chitosan coating of IONP result in interface that enhances ROS production, hence the antimicrobial activity.
authors.affiliation: Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India.
authors.forename: Manoranjan
authors.initials: M
authors.lastname: Arakha
authors.affiliation: Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India.
authors.forename: Sweta
authors.initials: S
authors.lastname: Pal
authors.affiliation: Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India.
authors.forename: Devyani
authors.initials: D
authors.lastname: Samantarrai
authors.affiliation: Department of Biotechnology, North Orissa University, Baripada, Odisha 757003, India.
authors.forename: Tapan K
authors.initials: TK
authors.lastname: Panigrahi
authors.affiliation: Department of Chemistry, Ravenshaw University, Cuttack, Odisha 753003, India.
authors.forename: Bairagi C
authors.initials: BC
authors.lastname: Mallick
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Odisha 769008, India.
authors.forename: Krishna
authors.initials: K
authors.lastname: Pramanik
authors.affiliation: Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India.
authors.forename: Bibekanand
authors.initials: B
authors.lastname: Mallick
authors.affiliation: Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Jha
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure; D048271:Chitosan / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure; D005290:Ferric Compounds / Q000737:chemistry* / Q000494:pharmacology; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000648:ultrastructure; D017382:Reactive Oxygen Species / Q000819:agonists* / Q000378:metabolism; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D017382:Reactive Oxygen Species; C000499:ferric oxide; D048271:Chitosan