title: The effects of caffeic, coumaric and ferulic acids on proliferation, superoxide production, adhesion and migration of human tumor cells in vitro.
abstract: Reactive oxygen species are well-known mediators of various biological responses. In this study, we examined the effect of three phenolic acids, caffeic, coumaric and ferulic acids, on superoxide anion production, adhesion and migration of human lung (A549) and colon adenocarcinoma (HT29-D4) cancer cell lines. Proliferation of both tumor cells was inhibited by phenolic acids. Caffeic, coumaric and ferulic acids also significantly inhibited superoxide production in A549 and HT29-D4 cells. Superoxide anion production decreased by 92% and 77% at the highest tested concentration (200 µM) of caffeic acid in A549 and HT29-D4 cell lines respectively. Furthermore, A549 and HT29-D4 cell adhesion was reduced by 77.9% and 79.8% respectively at the higher tested concentration of ferulic acid (200 µM). Migration assay performed towards A549 cell line, revealed that tested compounds reduced significantly cell migration. At the highest concentration tested (200 µM), the covered surface was 7.7%, 9.5% and 35% for caffeic, coumaric or ferulic acids, respectively. These results demonstrate that caffeic, coumaric and ferulic acids may participate as active ingredients in anticancer agents against lung and colon cancer development, at adhesion and migration steps of tumor progression.
authors.affiliation: Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir 5000, Tunisia; Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir 5000, Tunisia. Electronic address: nouha_nasr_1987@hotmail.fr.
authors.forename: Nouha
authors.initials: N
authors.lastname: Nasr Bouzaiene
authors.affiliation: Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir 5000, Tunisia; Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir 5000, Tunisia.
authors.forename: Soumaya
authors.initials: S
authors.lastname: Kilani Jaziri
authors.affiliation: Aix Marseille Université, INSERM, UMR_S 911, Faculté de Pharmacie, 27, Boulevard Jean Moulin, 13385 Cedex 05 Marseille, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Kovacic
authors.affiliation: Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir 5000, Tunisia; Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir 5000, Tunisia.
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
authors.affiliation: Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir 5000, Tunisia.
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.affiliation: Aix Marseille Université, INSERM, UMR_S 911, Faculté de Pharmacie, 27, Boulevard Jean Moulin, 13385 Cedex 05 Marseille, France.
authors.forename: José
authors.initials: J
authors.lastname: Luis
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002109:Caffeic Acids / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003373:Coumaric Acids / Q000494:pharmacology*; D006801:Humans; D013481:Superoxides / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D002109:Caffeic Acids; D003373:Coumaric Acids; D013481:Superoxides; C004999:ferulic acid; C040048:caffeic acid
keywords: Adhesion; Migration; Phenolic acids; Reactive oxygen species