title: Synergistic effect of exogeneous and endogeneous electrostimulation on osteogenic differentiation of human mesenchymal stem cells seeded on silk scaffolds.
abstract: Bioelectrical regulation of bone fracture healing is important for many cellular events such as proliferation, migration, and differentiation. The aim of this study was to investigate the osteogenic differentiation potential of human mesenchymal stem cells (hMSCs) cultivated on silk scaffolds in response to different modes of electrostimulation (e.g., exogeneous and/or endogeneous). Endogeneous electrophysiology was altered through the use of monensin (10 nM) and glibenclamide (10 μM), along with external electrostimulation (60 kHz; 100-500 mV). Monensin enhanced the expression of early osteogenic markers such as alkaline phosphatase (ALP) and runt-related transcription factor 2 (RUNX-2). When exogeneous electrostimulation was combined with glibenclamide, more mature osteogenic marker upregulation based on bone sialoprotein expression (BSP) and mineralization was found. These results suggest the potential to exploit both exogeneous and endogeneous biophysical control of cell functions towards tissue-specific goals.
authors.affiliation: Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, 02155, Massachusetts.|Bioengineering Division, Graduate School of Science and Engineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey.
authors.forename: Anıl S
authors.initials: AS
authors.lastname: Çakmak
authors.affiliation: Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, 02155, Massachusetts.|Nanotechnology and Nanomedicine Division, Graduate School of Science and Engineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey.
authors.forename: Soner
authors.initials: S
authors.lastname: Çakmak
authors.affiliation: Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, 02155, Massachusetts.
authors.forename: James D
authors.initials: JD
authors.lastname: White
authors.affiliation: Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, 02155, Massachusetts.
authors.forename: Waseem K
authors.initials: WK
authors.lastname: Raja
authors.affiliation: Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, 02155, Massachusetts.
authors.forename: Kyungsook
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, 02155, Massachusetts.
authors.forename: Sezin
authors.initials: S
authors.lastname: Yiğit
authors.affiliation: Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, 02155, Massachusetts.
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
authors.affiliation: Bioengineering Division, Graduate School of Science and Engineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey.|Nanotechnology and Nanomedicine Division, Graduate School of Science and Engineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey.|Department of Chemical Engineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey.
authors.forename: Menemşe
authors.initials: M
authors.lastname: Gümüşderelioğlu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D012831:Bombyx; D002118:Calcium / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D004558:Electric Stimulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D010012:Osteogenesis; D047011:Silk; D054457:Tissue Scaffolds
chemical_list: D047011:Silk; D000469:Alkaline Phosphatase; D002118:Calcium
keywords: endogeneous electrostimulation; human mesenchymal stem cells; osteogenic differentiation; silk scaffolds