title: Development of a Radiolabeled Peptide-Based Probe Targeting MT1-MMP for Breast Cancer Detection.
abstract: Breast cancer is one of the most frequent and aggressive primary tumors among women of all races. Matrix metalloproteinase (MMPs), a family of zinc- and calcium-dependent secreted or membrane anchored endopeptidases, is overexpressed in varieties of diseases including breast cancer. Therefore, noninvasive visualization and quantification of MMP in vivo are of great interest in basic research and clinical application for breast cancer early diagnosis. Herein, we developed a 99mTc labeled membrane type I matrix metalloproteinase (MT1-MMP) specific binding peptide, [99mTc]-(HYNIC-AF7p)(tricine)(TPPTS), for in vivo detection of MDA-MB-231 breast tumor by single photon emission computed tomography (SPECT). [99mTc]-(HYNIC-AF7p)(tricine)(TPPTS) demonstrated nice biostability and high MT1-MMP binding affinity in vitro and in vivo. Tumor-to-muscle ratio was found to reach to the highest (4.17±0.49) at 2 hour after intravenously administration of [99mTc]-(HYNIC-AF7P)(tricine)(TPPTS) into MDA-MB-231 tumor bearing mice. Overall, [99mTc]-(HYNIC-AF7P)(tricine)(TPPTS) demonstrated great potential for MT1-MMP targeted detection in vivo and it would be a promising molecular imaging probe that are probably beneficial to breast cancer early diagnoses.
authors.affiliation: Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Kaiyin
authors.initials: K
authors.lastname: Min
authors.affiliation: Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ji
authors.affiliation: Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Tiefeng
authors.initials: T
authors.lastname: Ji
authors.affiliation: Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of General Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Xuedong
authors.initials: X
authors.lastname: Fang
authors.affiliation: Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Qingjie
authors.initials: Q
authors.lastname: Ma
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D064593:Heterografts; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology; D053511:Matrix Metalloproteinase 14 / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000032:analysis*; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging*; D015609:Organotechnetium Compounds / Q000032:analysis* / Q000138:chemical synthesis / Q000493:pharmacokinetics / Q000633:toxicity; D019275:Radiopharmaceuticals / Q000032:analysis* / Q000138:chemical synthesis / Q000493:pharmacokinetics / Q000633:toxicity; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon; D014057:Tomography, X-Ray Computed; D016042:Transplantation, Heterotopic; D047368:Tumor Burden
chemical_list: D009363:Neoplasm Proteins; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; C000608366:technetium 99m (HYNIC-AF7p)(tricine)(TPPTS); C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14