title: MitoMiner v3.1, an update on the mitochondrial proteomics database.
abstract: Mitochondrial proteins remain the subject of intense research interest due to their implication in an increasing number of different conditions including mitochondrial and metabolic disease, cancer, and neuromuscular degenerative and age-related disorders. However, the mitochondrial proteome has yet to be accurately and comprehensively defined, despite many studies. To support mitochondrial research, we developed MitoMiner (http://mitominer.mrc-mbu.cam.ac.uk), a freely accessible mitochondrial proteomics database. MitoMiner integrates different types of subcellular localisation evidence with protein information from public resources, and so provides a comprehensive central resource for data on mitochondrial protein localisation. Here we report important updates to the database including the addition of subcellular immunofluorescent staining results from the Human Protein Atlas, computational predictions of mitochondrial targeting sequences, and additional large-scale mass-spectrometry and GFP tagging data sets. This evidence is shared across the 12 species in MitoMiner (now including Schizosaccharomyces pombe) by homology mapping. MitoMiner provides multiple ways of querying the data including simple text searches, predefined queries and custom queries created using the interactive QueryBuilder. For remote programmatic access, API's are available for several programming languages. This combination of data and flexible querying makes MitoMiner a unique platform to investigate mitochondrial proteins, with application in mitochondrial research and prioritising candidate mitochondrial disease genes.
authors.affiliation: MRC Mitochondrial Biology Unit, Wellcome Trust/MRC Building, Cambridge Biomedical Campus, Hills Road, Cambridge, CB2 0XY, UK.
authors.forename: Anthony C
authors.initials: AC
authors.lastname: Smith
authors.affiliation: MRC Mitochondrial Biology Unit, Wellcome Trust/MRC Building, Cambridge Biomedical Campus, Hills Road, Cambridge, CB2 0XY, UK ajr@mrc-mbu.cam.ac.uk.
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Robinson
mesh_terms: D000818:Animals; D002417:Cattle; D030562:Databases, Protein; D049452:Green Fluorescent Proteins / Q000032:analysis; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000737:chemistry; D021382:Protein Sorting Signals; D040901:Proteomics; D051381:Rats
chemical_list: D024101:Mitochondrial Proteins; D021382:Protein Sorting Signals; D049452:Green Fluorescent Proteins