title: Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy.
abstract: Current measurements of the biomechanical properties of cells require physical contact with cells or lack subcellular resolution. Here we developed a label-free microscopy technique based on Brillouin light scattering that is capable of measuring an intracellular longitudinal modulus with optical resolution. The 3D Brillouin maps we obtained of cells in 2D and 3D microenvironments revealed mechanical changes due to cytoskeletal modulation and cell-volume regulation.
authors.affiliation: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA.|Department of Dermatology, Harvard Medical School, Boston, Massachusetts, USA.|Fischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Scarcelli
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: William J
authors.identifier: http://orcid.org/0000-0003-2728-0746
authors.initials: WJ
authors.lastname: Polacheck
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Hadi T
authors.initials: HT
authors.lastname: Nia
authors.affiliation: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA.
authors.forename: Kripa
authors.initials: K
authors.lastname: Patel
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.|Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.|Department of Electrical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Grodzinsky
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.|Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Roger D
authors.initials: RD
authors.lastname: Kamm
authors.affiliation: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA.|Department of Dermatology, Harvard Medical School, Boston, Massachusetts, USA.|The Harvard-MIT Health Sciences and Technology Program, Cambridge, Massachusetts, USA.
authors.forename: Seok Hyun
authors.initials: SH
authors.lastname: Yun
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D048429:Cell Size; D003599:Cytoskeleton / Q000737:chemistry* / Q000648:ultrastructure; D055119:Elastic Modulus; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure; D021621:Imaging, Three-Dimensional; D051379:Mice; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D041681:NIH 3T3 Cells; D009997:Osmotic Pressure