title: Entamoeba histolytica Dmc1 Catalyzes Homologous DNA Pairing and Strand Exchange That Is Stimulated by Calcium and Hop2-Mnd1.
abstract: Meiosis depends on homologous recombination (HR) in most sexually reproducing organisms. Efficient meiotic HR requires the activity of the meiosis-specific recombinase, Dmc1. Previous work shows Dmc1 is expressed in Entamoeba histolytica, a eukaryotic parasite responsible for amoebiasis throughout the world, suggesting this organism undergoes meiosis. Here, we demonstrate Dmc1 protein is expressed in E. histolytica. We show that purified ehDmc1 forms presynaptic filaments and catalyzes ATP-dependent homologous DNA pairing and DNA strand exchange over at least several thousand base pairs. The DNA pairing and strand exchange activities are enhanced by the presence of calcium and the meiosis-specific recombination accessory factor, Hop2-Mnd1. In combination, calcium and Hop2-Mnd1 dramatically increase the rate of DNA strand exchange activity of ehDmc1. The biochemical system described herein provides a basis on which to better understand the role of ehDmc1 and other HR proteins in E. histolytica.
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina, United States of America.
authors.forename: Andrew A
authors.initials: AA
authors.lastname: Kelso
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina, United States of America.
authors.forename: Amanda F
authors.initials: AF
authors.lastname: Say
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina, United States of America.
authors.forename: Deepti
authors.initials: D
authors.lastname: Sharma
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina, United States of America.
authors.forename: LeAnna L
authors.initials: LL
authors.lastname: Ledford
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina, United States of America.
authors.forename: Audrey
authors.initials: A
authors.lastname: Turchick
authors.affiliation: Clemson University Genomics and Computational Biology Laboratory, Institute for Translational Genomics, Clemson, South Carolina, United States of America.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Saski
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, South Carolina, United States of America.
authors.forename: Ada V
authors.initials: AV
authors.lastname: King
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, South Carolina, United States of America.
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Attaway
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, South Carolina, United States of America; Clemson University School of Health Research, Clemson, South Carolina, United States of America; Eukaryotic Pathogens Innovation Center, Clemson University, Clemson, South Carolina, United States of America.
authors.forename: Lesly A
authors.initials: LA
authors.lastname: Temesvari
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina, United States of America; Clemson University School of Health Research, Clemson, South Carolina, United States of America; Center for Optical Materials Science and Engineering Technologies, Clemson University, Clemson, South Carolina, United States of America.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Sehorn
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000378:metabolism*; D059765:Homologous Recombination; D051379:Mice; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D015800:Protozoan Proteins; D000255:Adenosine Triphosphate; D004247:DNA; D002118:Calcium