title: Kinase active Misshapen regulates Notch signaling in Drosophila melanogaster.
abstract: Notch signaling pathway represents a principal cellular communication system that plays a pivotal role during development of metazoans. Drosophila misshapen (msn) encodes a protein kinase, which is related to the budding yeast Ste20p (sterile 20 protein) kinase. In a genetic screen, using candidate gene approach to identify novel kinases involved in Notch signaling, we identified msn as a novel regulator of Notch signaling. Data presented here suggest that overexpression of kinase active form of Msn exhibits phenotypes similar to Notch loss-of-function condition and msn genetically interacts with components of Notch signaling pathway. Kinase active form of Msn associates with Notch receptor and regulate its signaling activity. We further show that kinase active Misshapen leads to accumulation of membrane-tethered form of Notch. Moreover, activated Msn also depletes Armadillo and DE-Cadherin from adherens junctions. Thus, this study provides a yet unknown mode of regulation of Notch signaling by Misshapen.
authors.affiliation: Department of Molecular and Human Genetics, Banaras Hindu University, Varanasi 221005, India. Electronic address: abhinava83@gmail.com.
authors.forename: Abhinava K
authors.initials: AK
authors.lastname: Mishra
authors.affiliation: Department of Molecular and Human Genetics, Banaras Hindu University, Varanasi 221005, India. Electronic address: bhunalani@gmail.com.
authors.forename: Nalani
authors.initials: N
authors.lastname: Sachan
authors.affiliation: Department of Molecular and Human Genetics, Banaras Hindu University, Varanasi 221005, India. Electronic address: mousumi_mutsuddi@yahoo.com.
authors.forename: Mousumi
authors.initials: M
authors.lastname: Mutsuddi
authors.affiliation: Department of Molecular and Human Genetics, Banaras Hindu University, Varanasi 221005, India. Electronic address: ashim04@gmail.com.
authors.forename: Ashim
authors.initials: A
authors.lastname: Mukherjee
mesh_terms: D022005:Adherens Junctions; D000818:Animals; D051186:Armadillo Domain Proteins / Q000235:genetics / Q000378:metabolism; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D007124:Immunoenzyme Techniques; D008136:Longevity; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C495305:ARM protein, Drosophila; D051186:Armadillo Domain Proteins; D015820:Cadherins; D029721:Drosophila Proteins; C497759:N protein, Drosophila; D012333:RNA, Messenger; D051880:Receptors, Notch; D014157:Transcription Factors; C501889:shg protein, Drosophila; C113875:msn protein, Drosophila; D017346:Protein Serine-Threonine Kinases
keywords: Armadillo; DE-Cadherin; Drosophila; Misshapen; Notch; Signaling