title: CD1A-positive cells and HSP60 (HSPD1) levels in keratoacanthoma and squamous cell carcinoma.
abstract: CD1a is involved in presentation to the immune system of lipid antigen derived from tumor cells with subsequent T cell activation. Hsp60 is a molecular chaperone implicated in carcinogenesis by, for instance, modulating the immune reaction against the tumor. We have previously postulated a synergism between CD1a and Hsp60 as a key factor in the activation of an effective antitumor immune response in squamous epithelia. Keratoacantomas (KAs) are benign tumors that however can transform into squamous cell carcinomas (SCCs), but the reasons for this malignization are unknown. In a previous study, we found that CD1a-positive cells are significantly more numerous in KA than in SCC. In this study, we analyzed a series of KAs and SCCs by immunohistochemistry for CD1a and Hsp60. Our results show that the levels of both are significantly lower in KA than in SCC and support the hypothesis that KA may evolve towards SCC if there is a failure of the local modulation of the antitumor immune response. The data also show that immunohistochemistry for CD1a and Hsp60 can be of help in differential diagnosis between KAs and well-differentiated forms of SCC.
authors.affiliation: Pathology Institute, Department "G. D'Alessandro", University of Palermo, Palermo, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Cabibi
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore and IMET, Columbus Center, Baltimore, MD, USA.
authors.forename: Everly
authors.initials: E
authors.lastname: Conway de Macario
authors.affiliation: Pathology Institute, Department "G. D'Alessandro", University of Palermo, Palermo, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Ingrao
authors.affiliation: Pathology Institute, Department "G. D'Alessandro", University of Palermo, Palermo, Italy.
authors.forename: Rossana
authors.initials: R
authors.lastname: Porcasi
authors.affiliation: Pathology Institute, Department "G. D'Alessandro", University of Palermo, Palermo, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Zucco
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore and IMET, Columbus Center, Baltimore, MD, USA.|Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
authors.forename: Alberto J L
authors.initials: AJL
authors.lastname: Macario
authors.affiliation: Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.|Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Cappello
authors.affiliation: Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy. francyrappa@hotmail.com.|Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy. francyrappa@hotmail.com.|Department of Legal Science, Society and Sports, University of Palermo, Palermo, Italy. francyrappa@hotmail.com.
authors.forename: Francesca
authors.initials: F
authors.lastname: Rappa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018949:Antigens, CD1 / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000473:pathology*; D018834:Chaperonin 60 / Q000378:metabolism*; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D007636:Keratoacanthoma / Q000175:diagnosis / Q000473:pathology*; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000378:metabolism*; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D018949:Antigens, CD1; D014408:Biomarkers, Tumor; C419393:CD1a antigen; D018834:Chaperonin 60; C060293:HSPD1 protein, human; D024101:Mitochondrial Proteins
keywords: CD1a; Differential diagnosis; Hsp60; Immunohistochemistry; Keratoacantoma; Prognostic evaluation; Squamous cell carcinoma; Treatment