title: Concise Review: Fluorescent Reporters in Human Pluripotent Stem Cells: Contributions to Cardiac Differentiation and Their Applications in Cardiac Disease and Toxicity.
abstract: In the last decade, since the first report of induced pluripotent stem cells, the stem cell field has made remarkable progress in the differentiation to specialized cell-types of various tissues and organs, including the heart. Cardiac lineage- and tissue-specific human pluripotent stem cell (hPSC) reporter lines have been valuable for the identification, selection, and expansion of cardiac progenitor cells and their derivatives, and for our current understanding of the underlying molecular mechanisms. In order to further advance the use of hPSCs in the fields of regenerative medicine, disease modeling, and preclinical drug development in cardiovascular research, it is crucial to identify functionally distinct cardiac subtypes and to study their biological signaling events and functional aspects in healthy and diseased conditions. In this review, we discuss the various strategies that have been followed to generate and study fluorescent reporter lines in hPSCs and provide insights how these reporter lines contribute to a better understanding and improvement of cell-based therapies and preclinical drug and toxicity screenings in the cardiac field.
authors.affiliation: Department of Anatomy and Embryology, Leiden University Medical Centre, Leiden, The Netherlands.
authors.forename: Sabine C
authors.initials: SC
authors.lastname: Den Hartogh
authors.affiliation: Department of Anatomy and Embryology, Leiden University Medical Centre, Leiden, The Netherlands.|Department of Applied Stem cell Technologies. MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, P.O.Box 217, Enschede, The Netherlands.
authors.forename: Robert
authors.initials: R
authors.lastname: Passier
mesh_terms: D000818:Animals; D002318:Cardiovascular Diseases / Q000628:therapy*; D002454:Cell Differentiation; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D009206:Myocardium / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes
keywords: Cardiac differentiation; Cardiac toxicity; Cardiomyocyte subtype; Cell signaling; Cellular therapy; Drug development; Preclinical Screening