title: [Storage of Actinobacteria of the Genera Streptomyces and Nonomuraea by Low Temperature Preservation].
abstract: The influence of storage of actinobacteria Streptomyces hygroscopicus RIA 1433T, Nonomuraea roseoviolacea subsp. carminata INA 4281 and Nonomuraea sp. INA 34-06 at extremely low temperatures (-70 degrees C) for 1.5 years was studied with respect to their viability and antibiotic activity. The spores of the actinobacteria preserved their high viability when freezed at a concentration of 10(5)-10(7) CFU/ml. As for the antibiotic activity against the test culture Micrococcus luteus ATCC 9341, the strains differed: the S. hygroscopicus RIA 1433T colonies preserved their antibiotic activity against the test culture, the antibiotic activity of Nonomuraea roseoviolacea subsp. carminata lowered by 5% and that of N. sp. INA 34-06 lowered by 44%. Differences in the resistance of the strains to the storage at the extremely low temperatures were observed when the suspensions contained low concentrations of the spores (10(2) CFU/ml): S. hygroscopicus RIA 1433T preserved its viability and antibiotic activity during 1.5 years, while N. roseoviolacea subsp. carminata INA 4281 and N. sp. INA 34-06 lost the viability by the 8th month of the storage. The study showed that 10% glycerol solution used as a cryoprotector during the storage had no effect on viability and antibiotic activity of the actinobacteria.
authors.forename: O N
authors.initials: ON
authors.lastname: Sineva
authors.forename: N G
authors.initials: NG
authors.lastname: Kulikova
authors.forename: S N
authors.initials: SN
authors.lastname: Filippova
authors.forename: L P
authors.initials: LP
authors.lastname: Terekhova
mesh_terms: D039903:Actinobacteria / Q000254:growth & development* / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000737:chemistry; D005615:Freezing; D011309:Preservation, Biological / Q000379:methods*; D013171:Spores, Bacterial / Q000254:growth & development; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism
chemical_list: D003451:Cryoprotective Agents