title: Time--and Concentration--Dependent Effects of Resveratrol on miR 15a and miR16-1 Expression and Apoptosis in the CCRF-CEM Acute Lymphoblastic Leukemia Cell Line.
abstract: BACKGROUND
  Chemotherapy is one of the common approaches in treatment of cancers, especially leukemia. However, drug resistance phenomena reduce the likelihood of treatment success. Resveratrol is a herbal compound which through complicated processes makes some selected cells sensitive to treatment and induction of apoptosis. In the present study, the effects of resveratrol on the expression of miR 15a and miR16-1 and apoptosis in the CCRF-CEM cell line were investigated.
  
  
  METHODS
  The CCRF-CEM cell line was cultured under standard conditions and changes in miR 15a and miR 16-1 expression were analyzed by real time-PCR technique, with attention to reveratrol dose and time dependence. Also, apoptosis is evaluated by flow cytometry using annexin V and PI.
  
  
  RESULTS
  CCRF-CEM cells underwent dose-dependent apoptotic cell death in response to resveratrol. MiR 15a and miR 16-1 expression was up-regulated after 24 and 48 hours resveratrol treatment (p<0.05).
  
  
  CONCLUSIONS
  The results of our study indicate that resveratrol induces apoptosis in a time and dose- dependent manner in CCRF-CEM cells. Also, increased expression level of miR 16-1 and miR 15a by means of resveratrol in CCRF-CEM cells might have a role in apoptosis induction and predisposition. According to our results resveratrol can be regarded as a dietary supplement to improve efficacy of anti-leukemia therapies.
authors.affiliation: Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran E-mail : m.farshdousti@gmail.com.
authors.forename: Ako
authors.initials: A
authors.lastname: Azimi
authors.forename: Majid Farshdousti
authors.initials: MF
authors.lastname: Hagh
authors.forename: Mehdi
authors.initials: M
authors.lastname: Talebi
authors.forename: Bahman
authors.initials: B
authors.lastname: Yousefi
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Hossein pour feizi
authors.forename: Behzad
authors.initials: B
authors.lastname: Baradaran
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Movassaghpour
authors.forename: Karim
authors.initials: K
authors.lastname: Shamsasenjan
authors.forename: Taghi
authors.initials: T
authors.lastname: Khanzedeh
authors.forename: Abdol Hasan
authors.initials: AH
authors.lastname: Ghaderi
authors.forename: Milad Zadi
authors.initials: MZ
authors.lastname: Heydarabad
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy* / Q000235:genetics; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C505361:MIRN15 microRNA, human; C505362:MIRN16 microRNA, human; D035683:MicroRNAs; D013267:Stilbenes; D000077185:Resveratrol