title: Effectiveness of glucose-methanol extender for cryopreservation of Huso huso spermatozoa.
abstract: The present approach was designed to evaluate the methanol-glucose extender effects on sperm cryopreservation in beluga sturgeon, Huso huso. Sperm quality was examined by measuring post-thaw sperm motility and fertilizing rate at hatching stage. We first tested the effect of glucose concentration (0, 0.10, 0.15, 0.20 and 0.30M) in a methanol extender on post-thaw sperm motility. The optimal cryopreservation conditions were found to be 0.2M glucose in the extender. Then, motility and fertilization rates of sperm cryopreserved with 0.2M glucose and 10% methanol (GM) were compared to Tris-sucrose-KCl in 10% methanol extender (TSKM). Additionally, sperm motility and fertilizing ability in relation to 15 and 30min equilibration in GM extender before and after cryopreservation were measured. Higher post-thaw sperm motility duration and percentage as well as fertilization rate were obtained with the GM extender when compared to TSKM extender. Equilibration of sperm in extender did not affect the motility quality of either fresh-diluted or frozen/thawed sperm, while fertilization rate showed a significant decline alone after 30min of post-thaw storage. Our results indicated that the use of a simple extender consisting of 0.2M glucose in 10% methanol can be an alternative cryopreservation method to those previously described for sturgeons.
authors.affiliation: Fisheries Department, Natural Resources Faculty, Urmia University, Urmia, Iran. Electronic address: msaramli@gmail.com.
authors.forename: Mohammad Sadegh
authors.initials: MS
authors.lastname: Aramli
authors.affiliation: Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Karim
authors.initials: K
authors.lastname: Golshahi
authors.affiliation: Rajaee Sturgeon Propagation Center, Sari, Mazandaran, Iran.
authors.forename: Rajab Mohammad
authors.initials: RM
authors.lastname: Nazari
authors.affiliation: Medicine Laboratory, Alavi Educational and Treatment Center, Ardabil University of Medical Sciences, Ardabil, Iran.
authors.forename: Salim
authors.initials: S
authors.lastname: Aramli
authors.affiliation: Department of Aquaculture, Marine Science Faculty, Tarbiat Modares University, Noor, Mazandaran, Iran.
authors.forename: Ashkan
authors.initials: A
authors.lastname: Banan
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000627:therapeutic use*; D005260:Female; D005399:Fishes / Q000502:physiology; D005947:Glucose / Q000627:therapeutic use; D008297:Male; D000432:Methanol / Q000627:therapeutic use; D009994:Osmolar Concentration; D012661:Semen / Q000502:physiology; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology
chemical_list: D003451:Cryoprotective Agents; D005947:Glucose; D000432:Methanol
keywords: Cryopreservation; Extender; Fertilization; Glucose; Huso huso; Sperm motility