title: Discovery of Novel microRNAs in Aging Caenorhabditis elegans.
abstract: The rapid development of deep sequencing technologies over the last few years and concomitant increases in sequencing depth and cost efficiencies have opened the door to a ever-widening range of applications in biology-from whole-genome sequencing, to ChIP-seq analysis, epigenomic and RNA transcriptome surveys. Here we describe the application of deep sequencing to the discovery of novel microRNAs and characterization of their differential expression during adulthood in Caenorhabditis elegans.
authors.affiliation: Department of Biological Sciences, Quinnipiac University, Hamden, CT, 06518, USA.
authors.forename: Alexandre
authors.initials: A
authors.lastname: de Lencastre
authors.affiliation: Cancer Center at BI-Deaconess Medical Center and Department of Pathology, Institute for RNA Medicine, Harvard Medical School, 3 Blackfan Cir (CLS412), Boston, MA, 02115, USA. fslack@bidmc.harvard.edu.
authors.forename: Frank
authors.initials: F
authors.lastname: Slack
mesh_terms: D000375:Aging / Q000235:genetics*; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics*; D015203:Reproducibility of Results
chemical_list: D035683:MicroRNAs
keywords: Caenorhabditis elegans; Deep sequencing; Differential gene expression; microRNA