title: Identification of Tyrosine O Sulfated Proteins in Cow Retina and the 661W Cell Line.
abstract: Lack of tyrosine O Sulfation compromises both rod and cone electroretinographic responses emphasizing the importance of this post-translational modification for vision. To identify tyrosine sulfated proteins in retina, cow retinal lysates were subjected to immunoaffinity purification using an anti-sulfotyrosine antibody. The tyrosine sulfated proteins were eluted from the column using a sulfotyrosine pentapeptide and identified using mass spectrometry. Similarly, tyrosine sulfated proteins secreted by the 661W cell line were identified. Proteins identified were vitronectin, fibronectin, fibulin 2, nidogen, collagen V alpha 2, complement component 3 and C4 and fibrinogen beta. All proteins were subjected to analysis by 'Sulfinator' to determine potential sulfated tyrosines.
authors.affiliation: Departments of Cell Biology, University of Oklahoma Health Sciences Center, 73104, Oklahoma City, OK, USA. ykanan1@jhmi.edu.
authors.forename: Yogita
authors.initials: Y
authors.lastname: Kanan
authors.affiliation: Departments of Cell Biology, University of Oklahoma Health Sciences Center, 73104, Oklahoma City, OK, USA. muayyad-al-ubaidi@ouhsc.edu.
authors.forename: Muayyad R
authors.initials: MR
authors.lastname: Al-Ubaidi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002460:Cell Line; D002846:Chromatography, Affinity; D017077:Culture Media, Conditioned / Q000378:metabolism; D005136:Eye Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D011499:Protein Processing, Post-Translational; D012160:Retina / Q000378:metabolism*; D017949:Retinal Cone Photoreceptor Cells / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013431:Sulfates / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned; D005136:Eye Proteins; D013431:Sulfates; D014443:Tyrosine
keywords: 661W; PSG2; Posttranslational modification; Retina; Tyrosine sulfation