title: Glycoside hydrolase family 32 is present in Bacillus subtilis phages.
abstract: BACKGROUND
  Glycoside hydrolase family 32 (GH32) enzymes cleave the glycosidic bond between two monosaccharides or between a carbohydrate and an aglycone moiety. GH32 enzymes have been studied in prokaryotes and in eukaryotes but not in viruses.
  
  
  RESULTS
  This is the first analysis of GH32 enzymes in Bacillus subtilis phage SP10, ϕNIT1 and SPG24. Phylogenetic analysis, molecular docking and secretability predictions suggest that phage GH32 enzymes function as levan (fructose homopolysaccharide) fructotransferase.
  
  
  CONCLUSIONS
  We showed that viruses also contain GH32 enzymes and that our analyses in silico strongly suggest that these enzymes function as levan fructotransferase.
authors.affiliation: Institut de biologie intégrative et des systèmes (IBIS), Plate-Forme de Bio-Informatique, Université Laval, Pavillon Charles-Eugène Marchand, 1030 Avenue de la médecine, Québec, Québec, G1V 0A6, Canada. halim.maaroufi@ibis.ulaval.ca.
authors.forename: Halim
authors.initials: H
authors.lastname: Maaroufi
authors.affiliation: Institut de Biologie Intégrative et des Systèmes (IBIS) and Département de Microbiologie-Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, Québec, G1V 0A6, Canada.
authors.forename: Roger C
authors.initials: RC
authors.lastname: Levesque
mesh_terms: D000595:Amino Acid Sequence; D017103:Bacillus Phages / Q000201:enzymology* / Q000235:genetics*; D001412:Bacillus subtilis / Q000821:virology*; D016000:Cluster Analysis; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D006026:Glycoside Hydrolases