title: MOLECULAR CHARACTERIZATION OF AMERICAN CUTANEOUS LEISHMANIASIS IN THE TRI‑BORDER AREA OF ASSIS BRASIL, ACRE STATE, BRAZIL.
abstract: In this study, Leishmania species were identified by Polymerase Chain Reaction (PCR). The epidemiology of patients suspected of having American Cutaneous Leishmaniasis in the municipality of Assis Brasil, Acre State, located in the Brazil/Peru/Bolivia tri-border was also investigated. By PCR, the DNA of Leishmania was detected in 100% of the cases (37 samples) and a PCR-Restriction Fragment Length Polymorphism (RFLP) of the hsp 70 gene identified the species in 32 samples: Leishmania (Viannia) braziliensis (65.6%) , L. (V.) shawi (28.1%) , L. (V.) guyanensis (3.1%) and mixed infection L. (V.) guyanensis and L. (Leishmania) amazonensis(3.1%)This is the first report of L. (V.) shawi and L. (L.) amazonensis in Acre. The two predominant species were found in patients living in urban and rural areas. Most cases were found in males living in rural areas for at least three years and involved in rural work. This suggests, in most cases, a possible transmission of the disease from a rural/forest source, although some patients had not engaged in activities associated with permanence in forestall areas, which indicate a possible sandflies adaptation to the periurban setting.
authors.affiliation: Fundação Oswaldo Cruz, Porto Velho, Rondônia, BR, carolinateles@fiocruz.br, jmedeiro@gmail.com, paulaazevedo.2011@gmail.com, tonykatsuragawa@ fiocruz.br, licantanhede@gmail.com, ricardogodoi@fiocruz.br.
authors.forename: Carolina Bioni Garcia
authors.initials: CB
authors.lastname: Teles
authors.affiliation: Fundação Oswaldo Cruz, Porto Velho, Rondônia, BR, carolinateles@fiocruz.br, jmedeiro@gmail.com, paulaazevedo.2011@gmail.com, tonykatsuragawa@ fiocruz.br, licantanhede@gmail.com, ricardogodoi@fiocruz.br.
authors.forename: Jansen Fernandes
authors.initials: JF
authors.lastname: Medeiros
authors.affiliation: Fundação Oswaldo Cruz, Porto Velho, Rondônia, BR, carolinateles@fiocruz.br, jmedeiro@gmail.com, paulaazevedo.2011@gmail.com, tonykatsuragawa@ fiocruz.br, licantanhede@gmail.com, ricardogodoi@fiocruz.br.
authors.forename: Ana Paula de Azevedo dos
authors.initials: AP
authors.lastname: Santos
authors.affiliation: Centro de Pesquisa Gonçalo Muniz, Fundação Oswaldo Cruz, Salvador, Bahia, BR, lfreitas@bahia.fiocruz.br.
authors.forename: Luís Antônio Rodrigues de
authors.initials: LA
authors.lastname: Freitas
authors.affiliation: Fundação Oswaldo Cruz, Porto Velho, Rondônia, BR, carolinateles@fiocruz.br, jmedeiro@gmail.com, paulaazevedo.2011@gmail.com, tonykatsuragawa@ fiocruz.br, licantanhede@gmail.com, ricardogodoi@fiocruz.br.
authors.forename: Tony Hiroshi
authors.initials: TH
authors.lastname: Katsuragawa
authors.affiliation: Fundação Oswaldo Cruz, Porto Velho, Rondônia, BR, carolinateles@fiocruz.br, jmedeiro@gmail.com, paulaazevedo.2011@gmail.com, tonykatsuragawa@ fiocruz.br, licantanhede@gmail.com, ricardogodoi@fiocruz.br.
authors.forename: Lilian Motta
authors.initials: LM
authors.lastname: Cantanhêde
authors.affiliation: Fundação Oswaldo Cruz, Porto Velho, Rondônia, BR, carolinateles@fiocruz.br, jmedeiro@gmail.com, paulaazevedo.2011@gmail.com, tonykatsuragawa@ fiocruz.br, licantanhede@gmail.com, ricardogodoi@fiocruz.br.
authors.forename: Ricardo de Godoi Mattos
authors.initials: Rde G
authors.lastname: Ferreira
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, São Paulo, SP, BR, spider@icbusp.org.
authors.forename: Luís Marcelo Aranha
authors.initials: LM
authors.lastname: Camarg
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007891:Leishmania / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology*; D007897:Leishmaniasis, Mucocutaneous / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology*; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D012424:Rural Population; D014505:Urban Population; D055815:Young Adult