title: Variation in the ribosome interacting loop of the Sec61α from Giardia lamblia.
abstract: The interaction between the ribosome and the endoplasmic reticulum-located Sec61 protein translocon is mediated through an arginine residue of Sec61α, which is conserved in all prokaryotic and eukaryotic orthologues characterized to date. Using in silico approaches we report that instead of arginine, this ribosome-interaction function is most likely discharged by a lysine residue in the protist Giardia lamblia. This functional substitution of the R with a K in GlSec61α may have taken place to accommodate a G-rich rRNA.
authors.affiliation: Department of Biochemistry, Bose Institute, P-1/12 CIT Road, Scheme VII M, Kolkata, 700054,, West Bengal, India. abhishek.boseinst.84@gmail.com.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Sinha
authors.affiliation: Department of Biochemistry, Bose Institute, P-1/12 CIT Road, Scheme VII M, Kolkata, 700054,, West Bengal, India. atrayeeray@mail.jcbose.ac.in.
authors.forename: Atrayee
authors.initials: A
authors.lastname: Ray
authors.affiliation: Molecular Parasitology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T Road, Scheme XM, Kolkata, 700010,, West Bengal, India. gangulys@icmr.org.in.
authors.forename: Sandipan
authors.initials: S
authors.lastname: Ganguly
authors.affiliation: Bioinformatics Center, Bose Institute, P-1/12 CIT Scheme VII M, Kolkata, 700054,, West Bengal, India. sgd@jcbose.ac.in.
authors.forename: Shubhra
authors.initials: S
authors.lastname: Ghosh Dastidar
authors.affiliation: Department of Biochemistry, Bose Institute, P-1/12 CIT Road, Scheme VII M, Kolkata, 700054,, West Bengal, India. srimonti@jcbose.ac.in.
authors.forename: Srimonti
authors.initials: S
authors.lastname: Sarkar
mesh_terms: D003198:Computer Simulation; D004721:Endoplasmic Reticulum / Q000378:metabolism; D016829:Giardia lamblia / Q000235:genetics* / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D000069816:SEC Translocation Channels
chemical_list: D008565:Membrane Proteins; D015800:Protozoan Proteins; D000069816:SEC Translocation Channels; D008239:Lysine