title: Correlation of diffusion tensor and dynamic susceptibility contrast MRI with DNA ploidy and cell cycle analysis of gliomas.
abstract: OBJECTIVE
  Flow cytometry provides a powerful tool to assess cells in G0/G1, S and G2/M phase and ploidy. The purpose of the present study was to investigate the correlation between diffusion tensor (DTI) and dynamic susceptibility contrast (DSC) MRI metrics with cell cycle analysis findings in gliomas.
  
  
  METHODS
  We studied thirty patients who were operated on for glioma. DTI and DSC MRI were performed within a week prior to surgical excision. Lesion/normal ratios were calculated for the ADC, FA and rCBV. In an excised tumour sample flow cytometric analysis was performed.
  
  
  RESULTS
  There were 24 glioblastomas, 2 anaplastic astrocytomas, 1 oligoastrocytoma and 3 diffuse astrocytomas. There were significant differences between low and high-grade gliomas for rCBV and ADC values. Low grade tumours had higher G0/G1 phase fraction and lower S-phase, G2/M, S+G2/M and S+G2/M/G0/G1 fractions There was a significant negative correlation between rCBV and G0/G1 phase fraction and a positive correlation with G2/M, S+G2/M and the S+G2/M/G0/G1 fraction. Significant correlation was also observed between FA ratio and S+G2/M/G0/G1. There was a negative significant correlation between ADC and S+G2/M and the S+G2/M/G0/G1 fraction. There were 21 (70%) diploid and 9 (30%) aneuploid tumours. No significant difference was found between diploid and aneuploid tumours with respect to rCBV, ADC and FA values.
  
  
  CONCLUSIONS
  Dynamic susceptibility contrast MRI and diffusion tensor imaging metrics are correlated to tumour aggressiveness as assessed by cell cycle analysis.
authors.affiliation: Department of Radiology, University Hospital of Ioannina, Ioannina, Greece.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Zikou
authors.affiliation: Department of Neurosurgery, University Hospital of Ioannina, Ioannina, Greece. Electronic address: alexiougr@gmail.com.
authors.forename: George A
authors.initials: GA
authors.lastname: Alexiou
authors.affiliation: Haematology Laboratory-Unit of Molecular Biology, University Hospital of Ioannina, Ioannina, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Vartholomatos
authors.affiliation: Department of Pathology, University Hospital of Ioannina, Ioannina, Greece.
authors.forename: Anna
authors.initials: A
authors.lastname: Goussia
authors.affiliation: Department of Radiology, University Hospital of Ioannina, Ioannina, Greece.
authors.forename: Vasileios G
authors.initials: VG
authors.lastname: Xydis
authors.affiliation: Department of Radiology, University Hospital of Ioannina, Ioannina, Greece.
authors.forename: Paraskevi
authors.initials: P
authors.lastname: Kosta
authors.affiliation: Department of Neurosurgery, University Hospital of Ioannina, Ioannina, Greece.
authors.forename: Spyridon
authors.initials: S
authors.lastname: Voulgaris
authors.affiliation: Department of Neurology, University Hospital of Ioannina, Ioannina, Greece.
authors.forename: Athanasios P
authors.initials: AP
authors.lastname: Kyritsis
authors.affiliation: Department of Radiology, University Hospital of Ioannina, Ioannina, Greece.
authors.forename: Maria I
authors.initials: MI
authors.lastname: Argyropoulou
mesh_terms: D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D002453:Cell Cycle; D056324:Diffusion Tensor Imaging / Q000379:methods; D005260:Female; D005434:Flow Cytometry; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D011003:Ploidies
keywords: Cell cycle; Diffusion tensor imaging; Flow cytometry; Glioma; MRI; Perfusion imaging; Ploidy