title: Evaluation of antibacterial activity of caffeic acid encapsulated by β-cyclodextrins.
abstract: BACKGROUND
  Caffeic acid is described as antibacterial, but this bioactive molecule has some issues regarding solubility and stability to environmental stress. Thus, encapsulation devices are required.
  
  
  OBJECTIVE
  The aim of this work was to study the effect of the caffeic acid encapsulation by cyclodextrins on its antibacterial activity.
  
  
  METHODS
  The interactions between the caffeic acid and three cyclodextrins (β-cyclodextrin (βCD), 2-hydroxypropyl-β-cyclodextrin (HPβCD) and methyl-β-cyclodextrin were study.
  
  
  CONCLUSIONS
  The formation of an aqueous soluble inclusion complex was confirmed for βCD and HPβCD with a 1:1 stoichiometry. The βCD/caffeic acid complex showed higher stability than HPβCD/caffeic acid. Caffeic acid antibacterial activity was similar at pH 3 and pH 5 against the three bacteria (K. pneumoniae, S. epidermidis and S. aureus).
  
  
  CONCLUSIONS
  The antibacterial activity of the inclusion complexes was described here for the first time and it was shown that the caffeic acid activity was remarkably enhanced by the cyclodextrins encapsulation.
authors.affiliation: a CEB, Centre for Biological Engineering, LIBRO - Laboratório De Investigacão Em Biofilmes Rosário Oliveira, University of Minho , Campus Gualtar , Braga , Portugal and.|b Centre for Textile Science and Technology (2C2T), University of Minho, Azurém Campus , Guimarães , Portugal.
authors.forename: Eva
authors.initials: E
authors.lastname: Pinho
authors.affiliation: b Centre for Textile Science and Technology (2C2T), University of Minho, Azurém Campus , Guimarães , Portugal.
authors.forename: Graça
authors.initials: G
authors.lastname: Soares
authors.affiliation: a CEB, Centre for Biological Engineering, LIBRO - Laboratório De Investigacão Em Biofilmes Rosário Oliveira, University of Minho , Campus Gualtar , Braga , Portugal and.
authors.forename: Mariana
authors.initials: M
authors.lastname: Henriques
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000254:growth & development*; D002109:Caffeic Acids / Q000737:chemistry / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D006863:Hydrogen-Ion Concentration; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D002109:Caffeic Acids; D047392:beta-Cyclodextrins; C040048:caffeic acid
keywords: MIC; Staphylococcus aureus; inclusion complex; phenolic acid; wound infection