title: Tandem ion mobility spectrometry coupled to laser excitation.
abstract: This manuscript describes a new experimental setup that allows to perform tandem ion mobility spectrometry (IMS) measurements and which is coupled to a high resolution time-of-flight mass spectrometer. It consists of two 79 cm long drift tubes connected by a dual ion funnel assembly. The setup was built to permit laser irradiation of the ions in the transfer region between the two drift tubes. This geometry allows selecting ions according to their ion mobility in the first drift tube, to irradiate selected ions, and examine the ion mobility of the product ions in the second drift tube. Activation by collision is possible in the same region (between the two tubes) and between the second tube and the time-of-flight. IMS-IMS experiments on Ubiquitin are reported. We selected a given isomer of charge state +7 and explored its structural rearrangement following collisional activation between the two drift tubes. An example of IMS-laser-IMS experiment is reported on eosin Y, where laser irradiation was used to produce radical ions by electron photodetachment starting from doubly deprotonated species. This allowed measuring the collision cross section of the radical photo-product, which cannot be directly produced with an electrospray source.
authors.affiliation: Institut Lumière Matière, Université de Lyon, Université Lyon 1-CNRS, 69622 Villeurbanne cedex, France.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Simon
authors.affiliation: Institut des Sciences Analytiques, Université de Lyon, Université Lyon 1-CNRS, 69622 Villeurbanne cedex, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Chirot
authors.affiliation: Institut Lumière Matière, Université de Lyon, Université Lyon 1-CNRS, 69622 Villeurbanne cedex, France.
authors.forename: Chang Min
authors.initials: CM
authors.lastname: Choi
authors.affiliation: Institut Lumière Matière, Université de Lyon, Université Lyon 1-CNRS, 69622 Villeurbanne cedex, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Clavier
authors.affiliation: Institut Lumière Matière, Université de Lyon, Université Lyon 1-CNRS, 69622 Villeurbanne cedex, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Barbaire
authors.affiliation: Institut Lumière Matière, Université de Lyon, Université Lyon 1-CNRS, 69622 Villeurbanne cedex, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Maurelli
authors.affiliation: Institut Lumière Matière, Université de Lyon, Université Lyon 1-CNRS, 69622 Villeurbanne cedex, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Dagany
authors.affiliation: Institut Lumière Matière, Université de Lyon, Université Lyon 1-CNRS, 69622 Villeurbanne cedex, France.
authors.forename: Luke
authors.initials: L
authors.lastname: MacAleese
authors.affiliation: Institut Lumière Matière, Université de Lyon, Université Lyon 1-CNRS, 69622 Villeurbanne cedex, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Dugourd
mesh_terms: D004801:Eosine Yellowish-(YS) / Q000737:chemistry; D007834:Lasers; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D010084:Oxidation-Reduction; D055668:Photochemical Processes; D013696:Temperature; D013997:Time Factors; D025801:Ubiquitin / Q000737:chemistry
chemical_list: D025801:Ubiquitin; D004801:Eosine Yellowish-(YS)