title: Role of vascular endothelial growth factor in non‑small cell lung cancer pathogenesis.
abstract: The angiogenesis is an important process in the pathogenesis of malignancies. It is regulated by various growth factors, with the vascular endothelial growth factor (VEGF) playing the central role. The aim of the present study was to evaluate possible associations of functional VEGF -2578C>A, -634G>C, and +936C>T polymorphisms with the risk for occurrence and progression of non-small cell lung cancer (NSCLC) in patients living in Republic of Belarus.
  
  
  METHODS
  A total of 202 patients (147 males and 55 females) diagnosed as having the NSCLC. The control group consisted of 336 individuals (245 males and 91 females) without an oncopathology. The total DNA was isolated from peripheral blood. We investigated the single nucleotide polymorphisms of VEGF (rs 2010963), (rs 699947), (rs 3025039). The genotyping was performed by PCR-RFLP analysis.
  
  
  RESULTS
  Our results revealed a marginally significant association of the -2578CC genotype (p=0.002) with a greater degree of tumor spread (Т2-Т4). Heterozygous genotypes -2578СА and +936СT carriers were included into the follow-up group significantly more often (р=0.021 and р=0.012, respectively). Our study demonstrate that VEGF -2578A/C and +936C/T polymorphisms are among the factors determining the individual peculiarities of NSCLC course in this population and can be used for clarifying the prognosis of the disease.
authors.affiliation: Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk 20072, Republic of Belarus.
authors.forename: E V
authors.initials: EV
authors.lastname: Krupnova
authors.affiliation: Belarusian State Medical University, Minsk 220116, Republic of Belarus.
authors.forename: M N
authors.initials: MN
authors.lastname: Shapetska
authors.affiliation: Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk 20072, Republic of Belarus.
authors.forename: E P
authors.initials: EP
authors.lastname: Mikhalenko
authors.affiliation: Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk 20072, Republic of Belarus.
authors.forename: N V
authors.initials: NV
authors.lastname: Chebotaryova
authors.affiliation: Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk 20072, Republic of Belarus.
authors.forename: A N
authors.initials: AN
authors.lastname: Shchayuk
authors.affiliation: City Clinical Pathologoanatomic Bureau, Minsk 220116, Republic of Belarus.
authors.forename: S N
authors.initials: SN
authors.lastname: Pissarchik
authors.affiliation: Belarusian State Medical University, Minsk 220116, Republic of Belarus.
authors.forename: A V
authors.initials: AV
authors.lastname: Prokhorov
mesh_terms: D000483:Alleles; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D016022:Case-Control Studies; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D047368:Tumor Burden; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A