title: Proteomic Analysis to Elucidate the Antibacterial Action of Silver Ions Against Bovine Mastitis Pathogens.
abstract: Silver ions act as a powerful, broad-spectrum antimicrobial agent and are known to kill over 650 different kinds of pathogens. We investigated the protein expression pattern and identity after silver ion treatment in Escherichia coli and Staphylococcus aureus, which are primarily responsible for the majority of bovine mastitis cases using proteomics. Two-dimensional electrophoresis showed that silver ion treatment significantly reduced 5 spot's density in E. coli and S. aureus, respectively. We identified 10 proteins (alkyl hydroperoxide reductase C22 subunit, phosphoglucomutase, fructose-1-phosphate kinase, putative carbamoyl transferase, alpha-galactosidase, carbamate kinase, ornithine transcarbamoylase, fumarate hydratase class II, alcohol dehydrogenase, and conserved hypothetical protein) by matrix-assisted laser desorption ionization time of flight (MALDI-TOF). These results demonstrated that silver ions have bactericidal effects through energy deprivation, inhibition of DNA replication, and accumulation of oxidants in bovine mastitis pathogens and suggested that silver ions can be applied for the treatment of bovine mastitis.
authors.affiliation: National Institute of Animal Science, Rural Development Administration, Cheonan, 331-801, Republic of Korea.
authors.forename: Seog Jin
authors.initials: SJ
authors.lastname: Kang
authors.affiliation: National Institute of Animal Science, Rural Development Administration, Cheonan, 331-801, Republic of Korea.
authors.forename: Yong Il
authors.initials: YI
authors.lastname: Cho
authors.affiliation: National Institute of Animal Science, Rural Development Administration, Cheonan, 331-801, Republic of Korea.
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: National Institute of Animal Science, Rural Development Administration, Cheonan, 331-801, Republic of Korea. segi0486@korea.kr.
authors.forename: Eun Seok
authors.initials: ES
authors.lastname: Cho
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000032:analysis*; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D007477:Ions / Q000737:chemistry / Q000494:pharmacology; D008414:Mastitis, Bovine / Q000188:drug therapy / Q000382:microbiology*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D007477:Ions; D020543:Proteome; D012834:Silver
keywords: Bactericidal effect; Mastitis; Proteomics; Silver ion