title: Lvserpin3 is involved in shrimp innate immunity via the inhibition of bacterial proteases and proteases involved in prophenoloxidase system.
abstract: Serine protease inhibitor, represented by serpin, plays an important inhibitory role on proteases involved in the immune responses. To clarify the immune characterizations of serpin, a novel serpin (Lvserpin3) encoding for 410 amino acids with a 23-amino acid signal peptide and a serpin domain was identified from the Pacific white shrimp Litopenaeus vannamei. Lvserpin3 expressed strongest in hepatopancreas, and was significantly up-regulated in the early stage upon Vibrio anguillarum, Micrococcus lysodeikticus or White Spot Syndrome Virus (WSSV) infection. Suppression of Lvserpin3 by dsRNA led to a significant increase in the transcripts of LvPPAF, LvproPO and phenoloxidase (PO) activity, and also led to the high cumulative mortality. The recombinant Lvserpin3 protein (rLvserpin3) inhibited the proteases secreted by M. lysodeikticus and Bacillus subtilis, and further exhibited inhibitory role on the growth of B. subtilis and M. lysodeikticu. Moreover, rLvserpin3 was found to be able to block the activation of prophenoloxidase system. Taken together, the results imply that Lvserpin3 may be involved in shrimp innate immunity via the inhibition of bacterial proteases and proteases involved in prophenoloxidase system.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi, Yangling, 712100, China.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi, Yangling, 712100, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi, Yangling, 712100, China.
authors.forename: Fujun
authors.initials: F
authors.lastname: Hou
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi, Yangling, 712100, China.
authors.forename: Xianzong
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi, Yangling, 712100, China. Electronic address: liuxiaolin@nwsuaf.edu.cn.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004156:Catechol Oxidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004266:DNA Virus Infections / Q000276:immunology / Q000662:veterinary; D018076:DNA, Complementary / Q000235:genetics; D004792:Enzyme Precursors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016908:Gram-Positive Bacterial Infections / Q000276:immunology / Q000662:veterinary; D008837:Micrococcus / Q000254:growth & development; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D010447:Peptide Hydrolases / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA; D015842:Serine Proteinase Inhibitors / Q000235:genetics / Q000276:immunology*; D015843:Serpins / Q000276:immunology*; D014733:Vibrio / Q000254:growth & development; D014735:Vibrio Infections / Q000276:immunology / Q000662:veterinary; D046848:White spot syndrome virus 1
chemical_list: D060829:Arthropod Proteins; D001426:Bacterial Proteins; D018076:DNA, Complementary; D004792:Enzyme Precursors; D011994:Recombinant Proteins; D015842:Serine Proteinase Inhibitors; D015843:Serpins; C038451:pro-phenoloxidase; D004156:Catechol Oxidase; D010447:Peptide Hydrolases
keywords: Bacterial proteases; Litopenaeus vannamei; Prophenoloxidase system; Serpin3