title: In Vitro Safety/Protection Assessment of Resveratrol and Pterostilbene in a Human Hepatoma Cell Line (HepG2).
abstract: The aim of this work was to evaluate in vitro the genotoxic and/or antigenotoxic effects of resveratrol (RESV) and pterostilbene (PTER) on HepG2 cells. Moreover, additional tests were performed to evaluate early and late apoptosis events induced by the tested stilbenes. RESV and PTER did not show any genotoxic activity. As regards antigenotoxicity testing, RESV and PTER showed a typical, U-shaped hormetic dose-response relationship characterized by a biphasic trend with small quantities having opposite effects to large ones. HepG2 cells treated with PTER exhibited a marked increase in early apoptosis (40.1%) at 250 microM; whereas, the highest concentration tested for both RESV and PTER significantly increased the proportion of HepG2 cells undergoing late apoptosis (32.5 and 51.2%, respectively). The observed pro-apoptotic activity could, at least in part, explain the hormetic response observed when the compounds were tested for antigenotoxicity (i.e., in the presence of induced DNA damage).
authors.forename: Germana
authors.initials: G
authors.lastname: Lombardi
authors.forename: Samuele
authors.initials: S
authors.lastname: Vannini
authors.forename: Francesca
authors.initials: F
authors.lastname: Blasi
authors.forename: Maria Carla
authors.initials: MC
authors.lastname: Marcotullio
authors.forename: Luca
authors.initials: L
authors.lastname: Dominici
authors.forename: Milena
authors.initials: M
authors.lastname: Villarini
authors.forename: Lina
authors.initials: L
authors.lastname: Cossignani
authors.forename: Massimo
authors.initials: M
authors.lastname: Moretti
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000503:physiopathology*; D004249:DNA Damage / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000503:physiopathology*; D020011:Protective Agents / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D000077185:Resveratrol; D013267:Stilbenes / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D020011:Protective Agents; D013267:Stilbenes; C107773:pterostilbene; D000077185:Resveratrol