title: Up-regulation of miR-130b expression level and down-regulation of miR-218 serve as potential biomarker in the early detection of human osteosarcoma.
abstract: BACKGROUND
  Osteosarcoma (OS) is a primary malignant bone tumor with high morbidity that principally emerges in children and adolescents. MiRNAs regulate a variety of normal physiologic processes and are involved in tumorigenesis and development of multiple malignancies, including OS. This study was aimed to evaluate the clinical significance of miR-130b and miR-218 in osteosarcoma patient.
  
  
  METHODS
  We utilized quantitative real-time PCR to evaluate the level of miR-130b and miR-218 expressions in OS patients and normal tissues and their relationship with clinicopathological features and survival in OS patients.
  
  
  RESULTS
  QRT-PCR indicated that miR-130b expression in tumor tissues was strongly elevated than adjacent non-tumor tissues (P < 0.001), while the level of miR-218 expression in osteosarcoma tissues was down-regulated than adjacent non-tumor tissues (P < 0.001). We evaluated the clinical significance of miR-130b and miR-218 in osteosarcoma. Clinical correlation analysis showed that increased expression of miR-130b and decreased expression of miR-218 were significantly associated with advanced tumor stage (x (2) = 6.285, P < 0.009; x (2) = 7.172, P < 0.007), distant metastasis (x (2)  = 5.528; P < 0.001; x (2) = 4.617, P < 0.001) and size of tumor (x (2) = 5.01, P = 0.013; x (2) = 4.271, P = 0.019).
  
  
  CONCLUSIONS
  Taken together, our data indicated that high miR-130b level and low level of miR-218 are associated with poor clinicopathological characteristics. Furthermore, miR-130b may play a key role in the progression of osteosarcoma.
authors.affiliation: Department of Orthopedics Surgery, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Afshin
authors.initials: A
authors.lastname: Taheriazam
authors.affiliation: Department of Genetics, Faculty of Life Sciences, Azad University of Tehran Medical Sciences Branch, Tehran, Iran.
authors.forename: Amir Jouya
authors.initials: AJ
authors.lastname: Talaei
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.|Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Jamshidi
authors.affiliation: Department of Orthopaedic and Trauma Surgery, Birjand University of Medical Sciences, Birjand, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Shakeri
authors.affiliation: Department of Statistics, Faculty of Sciences, Islamic Azad University, Mashhad Branch, Mashhad, Iran.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Khoshbakht
authors.affiliation: Department of Molecular Biology, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Emad
authors.initials: E
authors.lastname: Yahaghi
authors.affiliation: Graduate, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. drshokrani.tums@gmail.com.
authors.forename: Marjan
authors.initials: M
authors.lastname: Shokrani
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D001706:Biopsy; D001859:Bone Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D016009:Chi-Square Distribution; D015536:Down-Regulation; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009367:Neoplasm Staging; D012516:Osteosarcoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D011237:Predictive Value of Tests; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C554019:MIRN130 microRNA, human; C547471:MIRN218 microRNA, human; D035683:MicroRNAs