title: Isolation and Genome Characterization of the Virulent Staphylococcus aureus Bacteriophage SA97.
abstract: A novel bacteriophage that infects S. aureus, SA97, was isolated and characterized. The phage SA97 belongs to the Siphoviridae family, and the cell wall teichoic acid (WTA) was found to be a host receptor of the phage SA97. Genome analysis revealed that SA97 contains 40,592 bp of DNA encoding 54 predicted open reading frames (ORFs), and none of these genes were related to virulence or drug resistance. Although a few genes associated with lysogen formation were detected in the phage SA97 genome, the phage SA97 produced neither lysogen nor transductant in S. aureus. These results suggest that the phage SA97 may be a promising candidate for controlling S. aureus.
authors.affiliation: Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, Korea. yjchang10@snu.ac.kr.
authors.forename: Yoonjee
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, Korea. hakdong.shin@gmail.com.
authors.forename: Hakdong
authors.initials: H
authors.lastname: Shin
authors.affiliation: Department of Food Science and Biotechnology, Kyung Hee University, Yongin 446-701, Korea. juhlee@khu.ac.kr.
authors.forename: Ju-Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Dermatology, College of Medicine, the Catholic University of Korea, Seoul 137-701, Korea. cjpark777smp@gmail.com.
authors.forename: Chul Jong
authors.initials: CJ
authors.lastname: Park
authors.affiliation: Department of Microbiology, College of Medicine, the Catholic University of Korea, Seoul 137-701, Korea. paik@catholic.ac.kr.
authors.forename: Soon-Young
authors.initials: SY
authors.lastname: Paik
authors.affiliation: Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, Korea. sangryu@snu.ac.kr.
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Ryu
mesh_terms: D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D023061:Gene Order; D016679:Genome, Viral; D008242:Lysogeny; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D011991:Receptors, Virus / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D017906:Siphoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D013204:Staphylococcus Phages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000821:virology*; D013682:Teichoic Acids / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: D004279:DNA, Viral; D011991:Receptors, Virus; D013682:Teichoic Acids
keywords: Staphylococcus aureus; bacteriophage; genome analysis