title: Effects of chronic exposure to tributyltin on tissue-specific cytochrome P450 1 regulation in juvenile common carp.
abstract: 1. The purpose of this study was to compare tributyltin (TBT)-induced cytochrome P450 1 (CYP450 1) regulation in liver, gills and muscle of juvenile common carp (Cyprinus carpio). 2. Fish were exposed to sublethal concentrations of TBT (75, 0.75 and 7.5 μg/L) for 60 days. CYP450 1A was measured at the enzyme activity level as 7-ethoxyresorufin-O-deethylase (EROD) activity, as well as the mRNA expression of CYP450 1 family genes (CYP1A, CYP1B, CYP1C1 and CYP1C2) in fish tissues. 3. Based on the results, the liver displayed the highest absolute levels of EROD activity, both under nonexposed and exposed conditions. Additional, EROD activities and CYP1A gene levels showed a good correlation in all three organs. According to the mRNA expression of CYP450 1 family genes, it suggested that CYP1A was to accommodate most EROD activity in fish, but other CYP450 forms also involved in this proceeding. 4. Overall, the study revealed both similarities and differences in the concentration-dependent CYP450 1 responses of the three target organs, which could provide useful information to better understand the mechanisms of TBT-induced bio-toxicity.
authors.affiliation: a Observation Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences , Wuhan , China .|b University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses , Vodňany , Czech Republic .
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Li
authors.affiliation: a Observation Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences , Wuhan , China .
authors.forename: Li-Qiao
authors.initials: LQ
authors.lastname: Zhong
authors.affiliation: a Observation Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences , Wuhan , China .|c College of Fisheries, Huazhong Agricultural University , Wuhan , Hubei , China , and.
authors.forename: Wei-Na
authors.initials: WN
authors.lastname: Mu
authors.affiliation: a Observation Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences , Wuhan , China .|d Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University , Chongqing , China.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Wu
mesh_terms: D000818:Animals; D002347:Carps / Q000235:genetics*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D004781:Environmental Exposure; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D016014:Linear Models; D009928:Organ Specificity / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014220:Trialkyltin Compounds / Q000633:toxicity*
chemical_list: D012333:RNA, Messenger; D014220:Trialkyltin Compounds; C011559:tributyltin; D003577:Cytochrome P-450 Enzyme System; D019363:Cytochrome P-450 CYP1A1
keywords: CYP450 1 family genes; EROD; fish; tissue-specific response; tributyltin