title: Micro-Attenuated Total Reflection Fourier Transform Infrared (Micro ATR FT-IR) Spectroscopic Imaging with Variable Angles of Incidence.
abstract: The control of the angle of incidence in attenuated total reflection (ATR) Fourier transform infrared (FT-IR) spectroscopy allows for the probing of the sample at different depths of penetration of the evanescent wave. This approach has been recently coupled with macro-imaging capability using a diamond ATR accessory. In this paper, the design of optical apertures for the micro-germanium (Ge) ATR objective is presented for an FT-IR spectroscopic imaging microscope, allowing measurements with different angles of incidence. This approach provides the possibility of three-dimensional (3D) profiling in micro-ATR FT-IR imaging mode. The proof of principle results for measurements of polymer laminate samples at different angles of incidence confirm that controlling the depth of penetration is possible using a Ge ATR objective with added apertures.
authors.affiliation: Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, Poland.
authors.forename: Tomasz P
authors.initials: TP
authors.lastname: Wrobel
authors.forename: Alessandra
authors.initials: A
authors.lastname: Vichi
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Baranska
authors.forename: Sergei G
authors.initials: SG
authors.lastname: Kazarian
mesh_terms: D004867:Equipment Design; D005857:Germanium / Q000737:chemistry; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D011108:Polymers / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation* / Q000379:methods
chemical_list: D011108:Polymers; D005857:Germanium