title: Fluorescent Molecular Tomography for In Vivo Imaging of Mouse Atherosclerosis.
abstract: Optical imaging technologies such as fluorescence molecular tomography (FMT) are gaining great relevance in cardiovascular research. The main reason is the increased number of available fluorescent agents, especially those termed "activatable probes," which remain quenched under baseline conditions and are fluorescent when a specific enzymatic activity is present. A major characteristic of FMT is the possibility of obtaining quantitative data of fluorescence signal distribution in a noninvasive fashion and using nonionizing radiation, making FMT an invaluable tool for longitudinal studies with biomedical applications. Here, we describe a standard procedure to perform FMT experiments in atherosclerosis mouse models, from the handling of the animals to the reconstruction of the 3D images.
authors.affiliation: Institute for Biomedical Engineering, Swiss Federal Institute of Technology (ETH) Zurich, Wolfgang-Pauli-Strasse 10, 8093, Zurich, Switzerland. aarranzdm@gmail.com.
authors.forename: Alicia
authors.initials: A
authors.lastname: Arranz
authors.affiliation: Institute for Biomedical Engineering, Swiss Federal Institute of Technology (ETH) Zurich, Wolfgang-Pauli-Strasse 10, 8093, Zurich, Switzerland.
authors.forename: Markus
authors.initials: M
authors.lastname: Rudin
authors.affiliation: Cardiovascular Research Unit, Hospital Ramón y Cajal, Universidad Francisco de Vitoria, 28223 Pozuelo de Alarcón, Madrid, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Zaragoza
authors.affiliation: Department of Bioengineering and Aerospace Engineering, Universidad Carlos III of Madrid, 28911, Madrid, Spain. jorge.ripoll@uc3m.es.|Experimental Medicine and Surgery Unit, Instituto de Investigación Sanitaria Gregorio Marañón, 28007, Madrid, Spain. jorge.ripoll@uc3m.es.
authors.forename: Jorge
authors.initials: J
authors.lastname: Ripoll
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D005456:Fluorescent Dyes; D020022:Genetic Predisposition to Disease; D007090:Image Interpretation, Computer-Assisted; D021621:Imaging, Three-Dimensional; D008163:Luminescent Measurements; D008822:Mice, Transgenic; D061848:Optical Imaging / Q000295:instrumentation / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes
keywords: 3D imaging; Activatable probes; Diffusion; Fluorescent agents; In vivo models; Near-infrared probes; Optical systems; Optical tomography; Scattering; Whole-body optical imaging