title: Thermotherapy-induced reduction in glioma invasiveness is mediated by tumor necrosis factor-alpha.
abstract: Thermotherapy has been proven to be effective for the treatment of various tumors, including glioma. We determined whether tumor necrosis factor-alpha (TNF-α) is involved in the regulation of the biological processes of glioma development. Reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry were used to investigate the levels of TNF-α mRNA and heat shock factor-1 (HSF1) protein, respectively, in glioma cells. Radioimmunoassay was used to dynamically monitor the contents of TNF-α in the nutrient fluid of C6 cells after thermotherapy treatment. Crystal violet staining was used to determine glioma invasiveness. The most obvious increases in HSF1 protein and TNF-α mRNA in C6 cells were observed at 30 and 60 min after thermotherapy, respectively. In addition, the radioactivity of TNF-α in the culture fluid of the C6 cells reached a peak after 120 min of thermotherapy. In addition, glioma invasiveness decreased and the concentration of TNF-α reached a maximum after 120 min of thermotherapy. Our results show that the decrease in thermotherapy-mediated glioma invasiveness is due to the accelerated release of TNF-α, which could promote the release of HSF1 from neurospongioma cells.
authors.affiliation: School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, China qinlijuan_l@163.com.
authors.forename: L J
authors.initials: LJ
authors.lastname: Qin
authors.affiliation: School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, China.
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.affiliation: College of Traditional Chinese Medicine, North China University of Science and Technology, Tangshan, China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Jia
authors.affiliation: School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, China.
authors.forename: Y B
authors.initials: YB
authors.lastname: Zhang
authors.affiliation: School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, China.
authors.forename: H T
authors.initials: HT
authors.lastname: Wang
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D002465:Cell Movement; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D000076249:Heat Shock Transcription Factors; D006979:Hyperthermia, Induced; D008297:Male; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; C000619709:Hsf1 protein, rat; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha