title: Comparison of Human Primary with Human iPS Cell-Derived Dopaminergic Neuron Grafts in the Rat Model for Parkinson's Disease.
abstract: Neuronal degeneration within the substantia nigra and the loss of the dopaminergic nigro-striatal pathway are the major hallmarks of Parkinson's disease (PD). Grafts of foetal ventral mesencephalic (VM) dopaminergic (DA) neurons into the striatum have been shown to be able to restore striatal dopamine levels and to improve overall PD symptoms. However, human foetus-derived cell grafts are not feasible for clinical application. Autologous induced pluripotent stem cell (iPS cell)-derived DA neurons are emerging as an unprecedented alternative. In this review, we summarize and compare the efficacy of human iPS cell-derived DA neuron grafts to restore normal behaviour in a rat model for PD with that of human foetal primary DA neurons. The differences we observed in the efficacy to restore normal function between the 2 types of DA neuron grafts could be ascribed to intrinsic properties of the iPS cell-derived DA neurons that critically affected survival and proper neurite extension in the striatum after implantation.
authors.affiliation: Center for Neuroscience, Shantou University Medical College, Shantou, Guangdong Province, People's Republic of China.|Department of Neuroscience, Medical Physiology, University Medical Center Groningen, University of Groningen, A. Deusinglaan 1, 9713 AV, Groningen, The Netherlands.
authors.forename: Su-Ping
authors.initials: SP
authors.lastname: Peng
authors.affiliation: Department of Neuroscience, Medical Physiology, University Medical Center Groningen, University of Groningen, A. Deusinglaan 1, 9713 AV, Groningen, The Netherlands. j.c.v.m.copray@umcg.nl.
authors.forename: Sjef
authors.initials: S
authors.lastname: Copray
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D004298:Dopamine / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D005333:Fetus; D006085:Graft Survival / Q000502:physiology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D015552:Injections, Intralesional; D017072:Neostriatum / Q000378:metabolism* / Q000473:pathology; D016627:Oxidopamine; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D061251:Primary Cell Culture; D051381:Rats; D014183:Transplantation, Heterologous
chemical_list: D016627:Oxidopamine; D004298:Dopamine
keywords: Foetal tissue; Neurite extension and Parkinson’s disease; Ventral mesencephalic dopaminergic neurons; iPS cells