title: Phytochemical and Biological Investigation of Two Diplotaxis Species Growing in Tunisia: D. virgata &amp; D. erucoides.
abstract: A phytochemical investigation of Diplotaxis virgata D.C. and D. erucoides (L.) D.C. (Brassicaceae) offered to the isolation of two new flavonoids isorhamnetin-3-O-α-l-glucopyranoside (1) and rhamnetin-3,3'-di-O-β-d-glucopyranoside (2), respectively. Their structures have been elucidated from the extended spectroscopic methods, including 1D- and 2D-NMR, UV and mass spectrometry analysis and by comparison with literature data. The fatty acid composition of the hexane extracts of the two species was also investigated by using GC-MS. The antioxidant activity of ethanol, ethyl acetate, n-butanol extracts and the isolated compounds from the two species was evaluated using DPPH and ABTS⁺ scavenging assays. All the tested samples showed an efficient radical scavenging ability, with IC50 values ranging from 16-40 µg/mL for the DPPH and from 17-44 µg/mL for the ABTS⁺ assays. In addition, the antibacterial activity of the prepared extracts and compounds 1 and 2, determined by well diffusion agar method against two Gram positive and five Gram negative bacteria, was evaluated and the results showed significant effects against all strains used.
authors.affiliation: Faculté des Sciences de Monastir, Université de Monastir, Avenue de l'Environnement, 5019 Monastir, Tunisia. bensalahnizar9@gmail.com.|Laboratoire de Chimie Organique Structurale, Synthèse et Etude Physicochimique-Faculté des Sciences de Tunis, 2092 El Manar, Tunisia. bensalahnizar9@gmail.com.
authors.forename: Nizar Ben
authors.initials: NB
authors.lastname: Salah
authors.affiliation: Service Central d'analyse, Institut des Sciences Analytiques, Université de Lyon, UMR 5280 CNRS, Université. herve.CASABIANCA@isa-lyon.fr.
authors.forename: Hervé
authors.initials: H
authors.lastname: Casabianca
authors.affiliation: Laboratoire de Chimie Hétérocyclique, Produits Naturels et Réactivité, Equipe, Chimie Médicinale et Produits Naturels, Faculté des Sciences de Monastir, Université de Monastir, Avenue of the Environment, 5019 Monastir, Tunisia. hich.benjannet@yahoo.fr.
authors.forename: Hichem Ben
authors.initials: HB
authors.lastname: Jannet
authors.affiliation: Service Central d'analyse, Institut des Sciences Analytiques, Université de Lyon, UMR 5280 CNRS, Université. s.chenavas@sca.cnrs.fr.
authors.forename: Sophie
authors.initials: S
authors.lastname: Chenavas
authors.affiliation: Service Central d'analyse, Institut des Sciences Analytiques, Université de Lyon, UMR 5280 CNRS, Université. Corinne.SANGLAR@isa-lyon.fr.
authors.forename: Corinne
authors.initials: C
authors.lastname: Sanglar
authors.affiliation: Service Central d'analyse, Institut des Sciences Analytiques, Université de Lyon, UMR 5280 CNRS, Université. Aurelie.FILDIER@isa-lyon.fr.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Fildier
authors.affiliation: Laboratoire de Chimie Organique Structurale, Synthèse et Etude Physicochimique-Faculté des Sciences de Tunis, 2092 El Manar, Tunisia. bouzouita.nabiha@gmail.com.
authors.forename: Nabiha
authors.initials: N
authors.lastname: Bouzouita
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D019607:Brassicaceae / Q000737:chemistry* / Q000145:classification; D005419:Flavonoids / Q000737:chemistry* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006586:Hexanes / Q000737:chemistry* / Q000494:pharmacology; D015394:Molecular Structure; D064209:Phytochemicals / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D014416:Tunisia
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D006586:Hexanes; D064209:Phytochemicals; D010936:Plant Extracts
keywords: D. erucoides; D. virgata; NMR; antimicrobial activity; antioxidant activity; fatty acids; flavonoids