title: Free DOX and chitosan-N-arginine conjugate stabilized indocyanine green nanoparticles for combined chemophotothermal therapy.
abstract: Indocyanine green (ICG) is a FDA-approved near-infrared (NIR) cyanine dye used in medical diagnostics. However, the utility of ICG remains limited by its unstable optical property, and concentration-dependent aggregation and precipitation. A chitosan-arginine conjugate (CS-N-Arg) was developed to increase the stability of ICG in physiological buffer saline via formation of strong electrostatic interactions between ICG and CS-N-Arg. The CS-N-Arg/ICG complex prevented ICG from aggregation and precipitation, thus it could serve as a theranostic nanomaterial for image-guided photothermal cancer therapy. The CS-N-Arg/ICG NPs showed excellent photostability, clear fluorescent images, and rapid temperature rise under laser irradiation. Cell viability assay indicated that CS-N-Arg/ICG NPs could efficiently suppress the growth of doxorubicin (DOX) resistant breast cancer cell (MCF-7/ADR cells) under NIR photothermal treatments. In combination of DOX with CS-N-Arg/ICG NPs, a combined effect was observed in MCF-7/ADR breast cancer cells due to dual hyperthermia and chemical therapeutic effects. The present observations suggest that CS-N-Arg/ICG NPs can effectively deliver ICG molecules to MCF-7/ADR breast cancer cells and overcome DOX resistance in the cells by hyperthermia.
authors.affiliation: Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Pei-Ru
authors.initials: PR
authors.lastname: Jheng
authors.affiliation: Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Kun-Ying
authors.initials: KY
authors.lastname: Lu
authors.affiliation: Department of Materials Science and Engineering, Vanung University, Chung-Li, Taiwan.
authors.forename: Shu-Huei
authors.initials: SH
authors.lastname: Yu
authors.affiliation: Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan; Department of Biochemistry and Molecular Cell Biology, School of Medicine, Taipei Medical University, Taipei 110, Taiwan. Electronic address: flmi530326@tmu.edu.tw.
authors.forename: Fwu-Long
authors.initials: FL
authors.lastname: Mi
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry*; D001120:Arginine / Q000737:chemistry*; D001943:Breast Neoplasms / Q000628:therapy; D048271:Chitosan / Q000737:chemistry*; D003131:Combined Modality Therapy; D004317:Doxorubicin / Q000737:chemistry*; D006801:Humans; D006979:Hyperthermia, Induced; D007208:Indocyanine Green / Q000737:chemistry*; D061986:MCF-7 Cells; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D053758:Nanoparticles; D010789:Phototherapy
chemical_list: D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin; D048271:Chitosan; D001120:Arginine; D007208:Indocyanine Green
keywords: Chitosan; Indocyanine green; Nanoparticles; Photothermal