title: Insulin-Like Growth Factor-2 Is Induced Following 5-Aminolevulinic Acid-Mediated Photodynamic Therapy in SW620 Human Colon Cancer Cell Line.
abstract: The IGF system is a family of polypeptide growth factors, which plays a significant role in the development and growth of many cells. Dysregulation of insulin-like growth factors and their pathway components has been connected with essential tumor properties, such as tumor cell proliferation, antiapoptotic properties, invasive behavior and chemotherapy resistance. However, the effects of photodynamic therapy (PDT), one of the cancer treatment methods for the regulation of the IGF signaling pathway, are still unclear. The aim of this study was to investigate the expression of IGF-2 after 5-aminolevulinic acid (5-ALA)-mediated-PDT in SW620 human colorectal cancer cells with evaluation of cell proliferation and apoptosis and to determine the effects of PDT on the IGF-2 receptor (IGF-2R), IGF-2 binding protein-1 (IGF-2BP-1) and the proapoptotic protein, BAX. Cells were treated with 5-aminolevulinic acid and its methyl ester. Changes of the expression and concentration of IGF-2 before and after treatment were assayed by immunocytochemistry, Western blot and ELISA. We found that IGF-2 was significantly overexpressed in the SW620 cell line, while its receptor and binding protein-1 were not significantly changed. Within this study, we would like to suggest that IGF-2 contributes to the effects of PDT and that its expression will influence post-PDT efficacy.
authors.affiliation: Department of Pathology, Wroclaw Medical University, Wrocław 50-368, Poland. marta1wozniak@wp.pl.
authors.forename: Marta
authors.initials: M
authors.lastname: Woźniak
authors.affiliation: Department of Pathology, Wroclaw Medical University, Wrocław 50-368, Poland. kamila.dus@gmail.com.
authors.forename: Kamila
authors.initials: K
authors.lastname: Duś-Szachniewicz
authors.affiliation: Department of Pathology, Wroclaw Medical University, Wrocław 50-368, Poland. ziolkows@interia.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Ziółkowski
mesh_terms: D000622:Aminolevulinic Acid / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics / Q000378:metabolism*; D010778:Photochemotherapy; D015854:Up-Regulation
chemical_list: C035170:IGF2 protein, human; D007335:Insulin-Like Growth Factor II; D000622:Aminolevulinic Acid
keywords: insulin-like growth factor-2; insulin-like growth factor-2 binding protein-1; insulin-like growth factor-2 receptor; photodynamic therapy