title: Massilia timonae as cause of chronic endophthalmitis following cataract surgery.
abstract: This is the first report of chronic postoperative endophthalmitis caused by Massilia timonae. The gram-negative bacillus was isolated from a patient who developed chronic endophthalmitis after cataract extraction. The microorganism was cultured on brain-heart infusion broth and identified using the 16S rRNA sequence analysis. Biochemically, the organism was positive for catalase and oxidase and negative for arginine dihydrolase. It was pansensitive to β-lactams, fluoroquinolones, aminoglycosides, and trimethoprim-sulfamethoxazole but resistant to aztreonam. Massilia timonae is a new bacterium implicated in postoperative chronic endophthalmitis. Eubacterial polymerase chain reaction was useful in identifying M timonae because phenotyping testing and conventional algorithms could not identify it.
  
  
  BACKGROUND
  No author has a financial or proprietary interest in any material or method mentioned.
authors.affiliation: From the Department of Ophthalmology (Chiquet, Aptel) and the Department of Microbiology (Boisset), Centre Hospitalier Universitaire de Grenoble, Grenoble Université Alpes, Grenoble, and the Department of Microbiology (Pechinot) and Department of Ophthalmology (Creuzot-Garcher, Bron), Centre Hospitalier Universitaire de Dijon, Dijon University, Dijon, France. Electronic address: cchiquet@chu-grenoble.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Chiquet
authors.affiliation: From the Department of Ophthalmology (Chiquet, Aptel) and the Department of Microbiology (Boisset), Centre Hospitalier Universitaire de Grenoble, Grenoble Université Alpes, Grenoble, and the Department of Microbiology (Pechinot) and Department of Ophthalmology (Creuzot-Garcher, Bron), Centre Hospitalier Universitaire de Dijon, Dijon University, Dijon, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Boisset
authors.affiliation: From the Department of Ophthalmology (Chiquet, Aptel) and the Department of Microbiology (Boisset), Centre Hospitalier Universitaire de Grenoble, Grenoble Université Alpes, Grenoble, and the Department of Microbiology (Pechinot) and Department of Ophthalmology (Creuzot-Garcher, Bron), Centre Hospitalier Universitaire de Dijon, Dijon University, Dijon, France.
authors.forename: Andre
authors.initials: A
authors.lastname: Pechinot
authors.affiliation: From the Department of Ophthalmology (Chiquet, Aptel) and the Department of Microbiology (Boisset), Centre Hospitalier Universitaire de Grenoble, Grenoble Université Alpes, Grenoble, and the Department of Microbiology (Pechinot) and Department of Ophthalmology (Creuzot-Garcher, Bron), Centre Hospitalier Universitaire de Dijon, Dijon University, Dijon, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Creuzot-Garcher
authors.affiliation: From the Department of Ophthalmology (Chiquet, Aptel) and the Department of Microbiology (Boisset), Centre Hospitalier Universitaire de Grenoble, Grenoble Université Alpes, Grenoble, and the Department of Microbiology (Pechinot) and Department of Ophthalmology (Creuzot-Garcher, Bron), Centre Hospitalier Universitaire de Dijon, Dijon University, Dijon, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Aptel
authors.affiliation: From the Department of Ophthalmology (Chiquet, Aptel) and the Department of Microbiology (Boisset), Centre Hospitalier Universitaire de Grenoble, Grenoble Université Alpes, Grenoble, and the Department of Microbiology (Pechinot) and Department of Ophthalmology (Creuzot-Garcher, Bron), Centre Hospitalier Universitaire de Dijon, Dijon University, Dijon, France.
authors.forename: Alain M
authors.initials: AM
authors.lastname: Bron
mesh_terms: D000368:Aged; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002442:Ceftazidime / Q000627:therapeutic use; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000032:analysis; D004359:Drug Therapy, Combination; D009877:Endophthalmitis / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D015818:Eye Infections, Bacterial / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D006801:Humans; D058449:Intravitreal Injections; D019654:Lens Implantation, Intraocular; D008826:Microbial Sensitivity Tests; D042802:Oxalobacteraceae / Q000235:genetics / Q000302:isolation & purification*; D018918:Phacoemulsification; D016133:Polymerase Chain Reaction; D011183:Postoperative Complications; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014031:Tobramycin / Q000627:therapeutic use; D014640:Vancomycin / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014640:Vancomycin; D002442:Ceftazidime; D014031:Tobramycin