title: Nano-colloid printing of functionalized PLA-b-PEO copolymers: tailoring the surface pattern of adhesive motif and its effect on cell attachment.
abstract: In this study, we investigate the preparation of surface pattern of functional groups on poly(lactide) (PLA) surfaces through the controlled deposition of core-shell self-assemblies based on functionalized PLA-b-PEO amphiphilic block copolymers from selective solvents. Through grafting RGDS peptide onto the functionalized copolymer surface, the presented approach enables to prepare PLA surfaces with random and clustered spatial distribution of adhesive motifs. The proposed topography of the adhesion motif was proved by atomic force microscopy techniques using biotin-tagged RGDS peptide grafted on the surface and streptavidin-modified gold nanospheres which bind the tagged RGDS peptides as a contrast agent. The cell culture study under static and dynamic conditions with MG63 osteosarcoma cell line showed that the clustered distribution of RGDS peptides provided more efficient initial cell attachment and spreading, and resistance to cell detachment under dynamic culture compared to randomly distributed RGDS motif when with the same average RGDS peptide concentration.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic. chanova@imc.cas.cz.
authors.forename: E
authors.initials: E
authors.lastname: Mázl Chánová
authors.forename: P
authors.initials: P
authors.lastname: Knotek
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: L
authors.initials: L
authors.lastname: Machová
authors.forename: V
authors.initials: V
authors.lastname: Proks
authors.forename: J
authors.initials: J
authors.lastname: Kučka
authors.forename: Š
authors.initials: Š
authors.lastname: Popelka
authors.forename: O
authors.initials: O
authors.lastname: Pop-Georgievski
authors.forename: A
authors.initials: A
authors.lastname: El Haj
authors.forename: D
authors.initials: D
authors.lastname: Kubies
authors.forename: F
authors.initials: F
authors.lastname: Rypáček
mesh_terms: D032701:Biomimetics; D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D006046:Gold; D006801:Humans; D007773:Lactates / Q000737:chemistry*; D053768:Metal Nanoparticles; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D009842:Oligopeptides; D011092:Polyethylene Glycols / Q000737:chemistry*; D011485:Protein Binding; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties
chemical_list: D007773:Lactates; D009842:Oligopeptides; C058523:polyethylene oxide-polylactic acid block copolymer; D011092:Polyethylene Glycols; D006046:Gold; D019809:Streptavidin; C046617:arginyl-glycyl-aspartyl-serine