title: Nonlinear electrical impedance spectroscopy of viruses using very high electric fields created by nanogap electrodes.
abstract: Our living sphere is constantly exposed to a wide range of pathogenic viruses, which can be either known, or of novel origin. Currently, there is no methodology for continuously monitoring the environment for viruses in general, much less a methodology that allows the rapid and sensitive identification of a wide variety of viruses responsible for communicable diseases. Traditional approaches, based on PCR and immunodetection systems, only detect known or specifically targeted viruses. We here describe a simple device that can potentially detect any virus between nanogap electrodes using nonlinear impedance spectroscopy. Three test viruses, differing in shape and size, were used to demonstrate the general applicability of this approach: baculovirus, tobacco mosaic virus (TMV), and influenza virus. We show that each of the virus types responded differently in the nanogap to changes in the electric field strength, and the impedance of the virus solutions differed depending both on virus type and virus concentration. These preliminary results show that the three virus types can be distinguished and their approximate concentrations determined. Although further studies are required, the proposed nonlinear impedance spectroscopy method may achieve a sensitivity comparable to that of more traditional, but less versatile, virus detection systems.
authors.affiliation: Department of Mechanical and Control Engineering, Tokyo Institute of Technology Tokyo, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Hatsuki
authors.affiliation: Faculty of Bioscience and Applied Chemistry, Housei University Tokyo, Japan.
authors.forename: Ayae
authors.initials: A
authors.lastname: Honda
authors.affiliation: Department of Bioscience, Teikyo University Tochigi, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kajitani
authors.affiliation: Department of Mechanical and Control Engineering, Tokyo Institute of Technology Tokyo, Japan.
authors.forename: Takatoki
authors.initials: T
authors.lastname: Yamamoto
keywords: environmental monitoring; impedance spectroscopy; nanofluidics; nanogap; virus; virus sensing