title: Screening and Identification of a Phage Display Derived Peptide That Specifically Binds to the CD44 Protein Region Encoded by Variable Exons.
abstract: CD44, especially the isoforms with variable exons (CD44v), is a promising biomarker for the detection of cancer. To develop a CD44v-specific probe, we screened a 7-mer phage peptide library against the CD44v3-v10 protein using an improved subtractive method. The consensus sequences with the highest frequency (designated CV-1) emerged after four rounds of panning. The binding affinity and specificity of the CV-1 phage and the synthesized peptide for the region of CD44 encoded by the variable exons were confirmed using enzyme-linked immunosorbent assay and competitive inhibition assays. Furthermore, the binding of the CV-1 probe to gastric cancer cells and tissues was validated using immunofluorescence and immunohistochemistry assays. CV-1 sensitively and specifically bound to CD44v on cancer cells and tissues. Thus, CV-1 has the potential to serve as a promising probe for cancer molecular imaging and target therapy.
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital of Medical School, Xian Jiaotong University, Xi'an, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Xi'an Medical University, Xi'an, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Jia
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Medical School, Xian Jiaotong University, Xi'an, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Life Science, Shaanxi Normal University, Xi'an, China.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Medical School, Xian Jiaotong University, Xi'an, China.
authors.forename: Shaoying
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital of Medical School, Xian Jiaotong University, Xi'an, China hesx123@126.com.
authors.forename: Shuixiang
authors.initials: S
authors.lastname: He
mesh_terms: D001435:Bacteriophages / Q000378:metabolism; D001667:Binding, Competitive / Q000502:physiology; D002460:Cell Line; D045744:Cell Line, Tumor; D005091:Exons; D005455:Fluorescent Antibody Technique / Q000379:methods; D057809:HEK293 Cells; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000378:metabolism
chemical_list: C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D019151:Peptide Library; D010455:Peptides
keywords: CD44v; cancer targeting; peptide probe; phage display