title: Correlation research on the protein expression (p75NTR, bax, bcl-2, and caspase-3) and cortical neuron apoptosis following mechanical injury in rat.
abstract: OBJECTIVE
  In this study, we aimed to survey the role of p75NTR, bax, bcl-2, and caspase-3 in the progress of traumatic brain injury (TBI).
  
  
  METHODS
  A mechanical trauma model of vital neurons was established by putting external pressure, contusion and centrifugal acceleration on neurons. Morphological change, survival rate, assay of LDH activity, and apoptosis rate were evaluated for mild, medium and severe injury models. The expression of bax, bcl-2, caspase-3, p75NTR, p75NTR mRNA was determined by immunohistochemistry, immunofluorescence, Western blotting and RT-PCR.
  
  
  RESULTS
  There was a transient high level Bcl-2 protein within 2 h after injury to increase neuronal tolerance and avoid apoptosis. Subsequently p75NTR, Bax/Bcl-2, and Caspase-3 reached their peaks from 48 to 72 h accompanied with the maximum apoptosis rate.
  
  
  CONCLUSIONS
  Our results suggest that apoptosis ratio in varying degree injury groups are correlated with the expression level of p75NTRmRNA, p75NTR, Caspase-3, Bax/Bcl-2 ratio.
authors.affiliation: Department of Orthopedic, Qilu Hospital, Shandong University, Jinan, China. yunzhenchen2011@hotmail.com.
authors.forename: H-C
authors.initials: HC
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: T
authors.initials: T
authors.lastname: Xin
authors.forename: W-H
authors.initials: WH
authors.lastname: Li
authors.forename: W-L
authors.initials: WL
authors.lastname: Wu
authors.forename: Q
authors.initials: Q
authors.lastname: Pang
authors.forename: Y-Z
authors.initials: YZ
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D001930:Brain Injuries / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D053148:Caspase 3 / Q000096:biosynthesis* / Q000235:genetics; D007150:Immunohistochemistry; D008297:Male; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D015996:Survival Rate; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D051017:Apoptosis Regulatory Proteins; C494828:Bax protein, rat; D009419:Nerve Tissue Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor; D051028:bcl-2-Associated X Protein; C068313:Ngfr protein, rat; D053148:Caspase 3