title: Fatty acid hydration activity of a recombinant Escherichia coli-based biocatalyst is improved through targeting the oleate hydratase into the periplasm.
abstract: Whole-cell biotransformation of fatty acids can be influenced by the activities of catalytic enzymes and by the efficiency of substrate transport into host cells. Here, we improved fatty acid hydration activity of the recombinant Escherichia coli expressing an oleate hydratase of Stenotrophomonas maltophilia by targeting the catalytic enzyme into the periplasm instead of the cytoplasm. Recombinant E. coli producing OhyA in the periplasm under guidance of the PelB signal sequence (E. coli OhyA_PP) exhibited significantly greater hydration activity with oleic acid and linoleic acid compared to a recombinant E. coli producing OhyA in the cytoplasm (E. coli OhyA_CS). For example, the oleate double bond hydration rate of E. coli OhyA_PP was >400 μmol/g dry cells/min (400 U/g dry cells), which is >10-fold higher than that of E. coli OhyA_CS. As the specific activities of the enzymes targeted into the cytoplasm and periplasm were comparable, we assumed that targeting OhyA into the periplasm could accelerate fatty acid transport to the catalytic enzymes by skipping the major mass transport barrier of the cytoplasmic membrane. Our results will contribute to the development of whole-cell biocatalysts for fatty acid biotransformation.
authors.affiliation: Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul, Republic of Korea.
authors.forename: Sang-Min
authors.initials: SM
authors.lastname: Jung
authors.affiliation: Department of Food Science & Engineering, Ewha Womans University, Seoul, Republic of Korea.
authors.forename: Joo-Hyun
authors.initials: JH
authors.lastname: Seo
authors.affiliation: Department of Food Science & Engineering, Ewha Womans University, Seoul, Republic of Korea.
authors.forename: Jung-Hoo
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Food Science & Engineering, Ewha Womans University, Seoul, Republic of Korea. jbpark06@ewha.ac.kr.
authors.forename: Jin-Byung
authors.initials: JB
authors.lastname: Park
authors.affiliation: Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul, Republic of Korea. jhseo94@snu.ac.kr.
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Seo
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D057005:Bioengineering; D003593:Cytoplasm / Q000378:metabolism; D004798:Enzymes / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005227:Fatty Acids / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism; D019897:Periplasm / Q000201:enzymology*; D011133:Polysaccharide-Lyases / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004798:Enzymes; D005227:Fatty Acids; D011994:Recombinant Proteins; D019301:Oleic Acid; D011133:Polysaccharide-Lyases; C023911:pectate lyase
keywords: FAD-dependent oleate hydratase; Fatty acids; Periplasmic expression; Whole cell biotransformation