title: Assessment of the Allergenic Potential of Transgenic Wheat (Triticum aestivum) with Reduced Levels of ω5-Gliadins, the Major Sensitizing Allergen in Wheat-Dependent Exercise-Induced Anaphylaxis.
abstract: The ω5-gliadins are the major sensitizing allergens in wheat-dependent exercise-induced anaphylaxis (WDEIA). In this study, two-dimensional immunoblot analysis was used to assess the allergenic potential of two transgenic wheat lines in which ω5-gliadin genes were silenced by RNA interference. Sera from 7 of 11 WDEIA patients showed greatly reduced levels of immunoglobulin E (IgE) reactivity to ω5-gliadins in both transgenic lines. However, these sera also showed low levels of reactivity to other gluten proteins. Sera from three patients showed the greatest reactivity to proteins other than ω5-gliadins, either high-molecular-weight glutenin subunits (HMW-GSs), α-gliadins, or non-gluten proteins. The complexity of immunological responses among these patients suggests that flour from the transgenic lines would not be suitable for individuals already diagnosed with WDEIA. However, the introduction of wheat lacking ω5-gliadins could reduce the number of people sensitized to these proteins and thereby decrease the overall incidence of this serious food allergy.
authors.affiliation: Western Regional Research Center, Agricultural Research Services (ARS), United States Department of Agriculture (USDA) , 800 Buchanan Street Albany, California 94710, United States.
authors.forename: Susan B
authors.initials: SB
authors.lastname: Altenbach
authors.affiliation: Western Regional Research Center, Agricultural Research Services (ARS), United States Department of Agriculture (USDA) , 800 Buchanan Street Albany, California 94710, United States.
authors.forename: Charlene K
authors.initials: CK
authors.lastname: Tanaka
authors.affiliation: UR1268 Biopolymers, Interactions, Assemblies, Institut National de la Recherche Agronomique (INRA) , Rue de la Géraudière, F-44316 Nantes Cedex 03, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Pineau
authors.affiliation: UR1268 Biopolymers, Interactions, Assemblies, Institut National de la Recherche Agronomique (INRA) , Rue de la Géraudière, F-44316 Nantes Cedex 03, France.
authors.forename: Roberta
authors.initials: R
authors.lastname: Lupi
authors.affiliation: Unité d'Allergologie Générale et de Pneumologie, Centre Hospitalier Universitaire (CHU) d'Angers , F-49933 Angers, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Drouet
authors.affiliation: Service d'Allergologie, Centre Hospitalier (CH) Epinal , F-88021 Epinal, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Beaudouin
authors.affiliation: Immunologie-Allergologie, Centre Hospitalier (CH) de Luxembourg , L-1210 Luxembourg, Luxembourg.
authors.forename: Martine
authors.initials: M
authors.lastname: Morisset
authors.affiliation: UR1268 Biopolymers, Interactions, Assemblies, Institut National de la Recherche Agronomique (INRA) , Rue de la Géraudière, F-44316 Nantes Cedex 03, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Denery-Papini
mesh_terms: D000328:Adult; D000707:Anaphylaxis / Q000097:blood / Q000276:immunology*; D052179:Antigens, Plant / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D015444:Exercise; D005260:Female; D005433:Flour / Q000032:analysis; D030841:Food, Genetically Modified; D005903:Gliadin / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D005983:Glutens / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D014908:Triticum / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D021182:Wheat Hypersensitivity / Q000097:blood / Q000276:immunology*
chemical_list: D052179:Antigens, Plant; D007073:Immunoglobulin E; D005983:Glutens; D005903:Gliadin; C037302:glutenin
keywords: RNA interference; Triticum aestivum; WDEIA; food allergy; gene silencing; gliadins; gluten proteins; immunoblots; wheat