title: Composition, in vitro Anti-inflammatory, Antioxidant and Antimicrobial Activities of Essential Oils from Leaf and Twig Parts of Cupressus cashmeriana.
abstract: The chemical composition and in vitro anti-inflammatory, antioxidant and antimicrobial activities of the essential oils isolated from the leaf and twig of Cupressus cashmeriana have been investigated. The essential oils were isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS, respectively. The leaf oil mainly consisted of alpha-pinene (21.8%), epizonarene (8.0%), sabinene (7.9%), limonene (7.6%), gamma-terpinene (7.0%), and allo-aromadendrene (7.0%); the twig oil was mostly carvacrol methyl ether (35.4%), manool (16.1%), carvacrol (14.2%), and (2Z,6E)-farnesol (6.9%). Twig oil, but not leaf oil, was able to reduce nitric oxide production by lipopolysaccharide-activated murine macrophages RAW 264.7 without reducing the cell viability. In addition, twig oil showed better antioxidant and antimicrobial activities than leaf oil. The major ingredient of the twig oil that was responsible for the anti-inflammatory, antioxidant and antimicrobial activities was carvacrol.
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Su
authors.forename: Kuan-Ping
authors.initials: KP
authors.lastname: Hsu
authors.forename: Kuo-Feng
authors.initials: KF
authors.lastname: Hua
authors.forename: Chen-Lung
authors.initials: CL
authors.lastname: Ho
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002460:Cell Line; D029781:Cupressus / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D009822:Oils, Volatile; D010938:Plant Oils