title: Turn-On Ratiometric Fluorescent Probe for Selective Discrimination of Cr(3+) from Fe(3+) in Aqueous Media for Living Cell Imaging.
abstract: Pyrene-based turn-on ratiometric fluorescent probe 1 demonstrates high sensitivity and exceptional selectivity toward Cr(3+) in the presence of other metals, including Fe(3+) in aqueous media. Interaction of Cr(3+) with probe 1 brings pyrene moieties close enough to have better aligned π-π stacking, thus enhancing the excimer peak many fold. On the other hand, the interaction of Fe(3+) with probe 1 brings forth a negligible difference in stacking, resulting in an insignificant change in fluorescence intensity. Exceptional selectivity of probe 1 with Cr(3+) over Fe(3+) and other metals has been confirmed by theoretical studies in addition to experimental results. Imaging of HeLa cells observed by confocal fluorescence microscopy reveals that probe 1 can be used to monitor Cr(3+) in live cells to map its subcellular distribution.
authors.affiliation: Center for Superfunctional Materials, Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798 (Republic of Korea).
authors.forename: Lubna
authors.initials: L
authors.lastname: Rasheed
authors.affiliation: Center for Superfunctional Materials, Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798 (Republic of Korea).|Department of Chemistry, Pohang University of Science and Technology, Pohang, 790-784 (Republic of Korea).
authors.forename: Muhammad
authors.initials: M
authors.lastname: Yousuf
authors.affiliation: Center for Superfunctional Materials, Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798 (Republic of Korea).|Department of Chemistry, Pohang University of Science and Technology, Pohang, 790-784 (Republic of Korea).
authors.forename: Il Seung
authors.initials: IS
authors.lastname: Youn
authors.affiliation: Center for Superfunctional Materials, Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798 (Republic of Korea).
authors.forename: Taeseung
authors.initials: T
authors.lastname: Yoon
authors.affiliation: School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan 689-798 (Republic of Korea).
authors.forename: Kwang-Youn
authors.initials: KY
authors.lastname: Kim
authors.affiliation: School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan 689-798 (Republic of Korea).
authors.forename: Young-Kyo
authors.initials: YK
authors.lastname: Seo
authors.affiliation: Center for Superfunctional Materials, Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798 (Republic of Korea).|Department of Chemistry, Pohang University of Science and Technology, Pohang, 790-784 (Republic of Korea).
authors.forename: Genggongwo
authors.initials: G
authors.lastname: Shi
authors.affiliation: Center for Superfunctional Materials, Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798 (Republic of Korea).|Department of Chemistry, Pohang University of Science and Technology, Pohang, 790-784 (Republic of Korea).
authors.forename: Muhammad
authors.initials: M
authors.lastname: Saleh
authors.affiliation: UNIST Central Research Facilities (UCRF), Ulsan National Institute of Science and Technology, Ulsan 689-798 (Republic of Korea).
authors.forename: Jin-Hoe
authors.initials: JH
authors.lastname: Hur
authors.affiliation: Center for Superfunctional Materials, Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798 (Republic of Korea). kimks@unist.ac.kr.
authors.forename: Kwang S
authors.initials: KS
authors.lastname: Kim
mesh_terms: D002857:Chromium / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000008:administration & dosage* / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007501:Iron / Q000737:chemistry*; D018613:Microscopy, Confocal; D015394:Molecular Structure; D011721:Pyrenes / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D005456:Fluorescent Dyes; D011721:Pyrenes; D002857:Chromium; C030984:pyrene; D007501:Iron
keywords: chromium; density functional theory; fluorescence; pyrene; ratiometric