title: Transposon-Assisted Genetic Engineering with Mos1-Mediated Single-Copy Insertion (MosSCI).
abstract: Transgenesis in model organisms is necessary to determine the function, expression, and subcellular localization of gene products. In Caenorhabditis elegans, injected DNA can be propagated as multicopy extrachromosomal arrays but transgenes in arrays are mosaic, over-expressed in some tissues and silenced in the germline. Here, a method to insert a transgene into a specific genomic location called Mos1-mediated single-copy insertion (MosSCI) is described. Single-copy insertion allows transgene expression at levels that approximate endogenous gene expression as well as expression in the germline.
authors.affiliation: Howard Hughes Medical Institute, University of Utah, 257 S 1400 E RM 201, Salt Lake City, UT, 84112, USA. christianfj@gmail.com.|Department of Biology, University of Utah, 257 S 1400 E RM 201, Salt Lake City, UT, 84112, USA. christianfj@gmail.com.|Danish National Research Foundation Centre for Cardiac Arrhythmia, University of Copenhagen, Copenhagen, Denmark. christianfj@gmail.com.
authors.forename: Christian
authors.initials: C
authors.lastname: Frøkjær-Jensen
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000378:metabolism*; D018628:Gene Dosage; D023061:Gene Order; D018390:Gene Targeting; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D016254:Mutagenesis, Insertional; D015203:Reproducibility of Results; D019895:Transposases / Q000378:metabolism*
chemical_list: D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; C111652:mariner transposases; D019895:Transposases
keywords: Endogenous levels of gene expression; Germline expression; Mos1 single-copy insertion (MosSCI); Mos1 transposon; Transgenesis; Universal insertion sites