title: Evaluation of an immunochromatographic assay for direct identification of thermostable direct hemolysin-producing Vibrio parahaemolyticus colonies on selective agar plates.
abstract: We evaluated the utility of an immunochromatographic assay (NH IC TDH) in identifying thermostable direct hemolysin (TDH)-producing Vibrio parahaemolyticus colonies on selective agar plates. The sensitivity of the NH IC TDH assay was 100% (189 samples) and its specificity was 100% (41 samples) compared with the presence of tdh.
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi 1-chome, Higashinari-ku, Osaka 537-0025, Japan. Electronic address: kawatu@iph.pref.osaka.jp.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kawatsu
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi 1-chome, Higashinari-ku, Osaka 537-0025, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Sakata
authors.affiliation: R&D Center, NH Foods Ltd., 3-3 Midorigahara, Tsukuba, Ibaraki 300-2646, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Yonekita
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi 1-chome, Higashinari-ku, Osaka 537-0025, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kumeda
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods*; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D014735:Vibrio Infections / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D006460:Hemolysin Proteins; C068489:thermostable direct hemolysin
keywords: Diarrhea; Food-borne pathogen; Immunochromatographic assay; TDH-producing Vibrio parahaemolyticus