title: Gut morphology and hepatic oxidative status of European sea bass (Dicentrarchus labrax) juveniles fed plant feedstuffs or fishmeal-based diets supplemented with short-chain fructo-oligosaccharides and xylo-oligosaccharides.
abstract: The effects of short-chain fructo-oligosaccharides (scFOS) and xylo-oligosaccharides (XOS) on gut morphology and hepatic oxidative status were studied in European sea bass juveniles weighing 60 g. Fish were fed diets including fishmeal (FM diets) or plant feedstuffs (PF diets; 30 FM:70 PF) as main protein sources (control diets). Four other diets were formulated similar to the control diets but including 1 % scFOS or 1 % XOS. At the end of the trial, fish fed PF-based diets presented histomorphological alterations in the distal intestine, whereas only transient alterations were observed in the pyloric caeca. Comparatively to fish fed FM-based diets, fish fed PF diets had higher liver lipid peroxidation (LPO), superoxide dismutase (SOD) and catalase (CAT) activities, and lower glutathione peroxidase, glutathione reductase and glucose 6-phosphate dehydrogenase activities. In fish fed the PF diets, prebiotic supplementation decreased SOD activity and XOS supplementation further decreased CAT activity. In fish fed the FM diets, XOS supplementation promoted a reduction of all antioxidant enzyme activities. Overall, dietary XOS and scFOS supplementation had only minor effects on gut morphology or LPO levels. However, dietary XOS reduced antioxidant enzymatic activity in both PF and FM diets, which indicate a positive effect on reduction of hepatic reactive oxygen species production.
authors.affiliation: 1Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR),Universidade do Porto,Rua dos Bragas 289,4050-123 Porto,Portugal.
authors.forename: Inês
authors.initials: I
authors.lastname: Guerreiro
authors.affiliation: 1Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR),Universidade do Porto,Rua dos Bragas 289,4050-123 Porto,Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Couto
authors.affiliation: 1Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR),Universidade do Porto,Rua dos Bragas 289,4050-123 Porto,Portugal.
authors.forename: Amalia
authors.identifier: http://orcid.org/0000-0002-9971-8623
authors.initials: A
authors.lastname: Pérez-Jiménez
authors.affiliation: 1Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR),Universidade do Porto,Rua dos Bragas 289,4050-123 Porto,Portugal.
authors.forename: Aires
authors.initials: A
authors.lastname: Oliva-Teles
authors.affiliation: 1Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR),Universidade do Porto,Rua dos Bragas 289,4050-123 Porto,Portugal.
authors.forename: Paula
authors.initials: P
authors.lastname: Enes
mesh_terms: D000821:Animal Feed; D000818:Animals; D001492:Bass; D004798:Enzymes / Q000378:metabolism; D005965:Glucuronates / Q000008:administration & dosage*; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D009844:Oligosaccharides / Q000008:administration & dosage*; D018384:Oxidative Stress; D056692:Prebiotics; D011706:Pyloric Antrum / Q000033:anatomy & histology / Q000378:metabolism*
chemical_list: D004798:Enzymes; D005965:Glucuronates; D009844:Oligosaccharides; D056692:Prebiotics; C116580:fructooligosaccharide; C570991:xylooligosaccharide
keywords: Antioxidant defences; CAT catalase; DI distal intestine; European sea bass; FM fishmeal; FMC diet FM control diet; FOS fructo-oligosaccharides; Fish health; G6PD glucose 6-phosphate dehydrogenase; GPX glutathione peroxidase; GR glutathione reductase; Intestinal histology; LPO lipid peroxidation; MOS mannan-oligosaccharides; PC pyloric caeca; PF plant feedstuffs; Plant feedstuffs; Prebiotics; ROS reactive oxygen species; SBM soyabean meal; SOD superoxide dismutase; XOS xylo-oligosaccharides; scFOS short-chain fructo-oligosaccharides