title: Safe sequencing depth to estimate the intra-host heterogeneity of viruses.
abstract: Massively parallel sequencing allows efficient determination of genomic sequences and intra-host polymorphisms for viruses. However, the sequencing depth that guarantees safe interpretation of the observed data is unclear. We demonstrated that 10-fold genome coverage may allow safe genome assembly, and 1000-fold coverage is required to obtain reliable polymorphism estimation.
authors.forename: Xianwen
authors.initials: X
authors.lastname: Ren
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
mesh_terms: D029043:Ebolavirus / Q000145:classification* / Q000235:genetics*; D016679:Genome, Viral; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*
keywords: Ebola virus; coverage; deep sequencing; genome sequencing; heterogeneity