title: Real-time monitoring of mesenchymal stem cell responses to biomaterial surfaces and to a model drug by using quartz crystal microbalance.
abstract: In this study, the mesenchymal stem cell (MSC) responses to biomaterial surfaces and to an anti-microtubule drug (vinblastine) were detected by using the quartz crystal microbalance (QCM). Gold electrodes with different coatings were subjected to MSCs under flow conditions; thus, crystal frequency decreased due to the adhesion of MSCs on the crystal. For evaluation of cell-drug interactions, vinblastine was introduced to MSCs attached onto the surfaces. The changes in frequency indicated the binding of drug to cell microtubules. The present study demonstrates the suitability of QCM as an invaluable tool for the real-time monitoring of cell-surface and cell-drug interactions.
authors.affiliation: a Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory , Ankara University Faculty of Science, and Ankara University Stem Cell Institute , Ankara , Turkey.
authors.forename: Şükran
authors.initials: Ş
authors.lastname: Şeker
authors.affiliation: a Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory , Ankara University Faculty of Science, and Ankara University Stem Cell Institute , Ankara , Turkey.
authors.forename: A Eser
authors.initials: AE
authors.lastname: Elçin
authors.affiliation: a Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory , Ankara University Faculty of Science, and Ankara University Stem Cell Institute , Ankara , Turkey.
authors.forename: Y Murat
authors.initials: YM
authors.lastname: Elçin
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000648:ultrastructure; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar
keywords: Biomaterials; cell–drug interactions; cell–surface interactions; mesenchymal stem cells; quartz crystal microbalance