title: Electron Tomography Methods for C. elegans.
abstract: Methods for electron tomography of the nematode C. elegans are explained in detail, including a brief introduction to specimen preparation, methods for image collection, and a comparison of several general methods for producing dual-axis tomograms, with or without external fiducial reference objects. New electron tomograms highlight features in software for data display, annotation, and analysis. This chapter discusses the ultrastructural analysis of cells and tissues, rather than molecular studies.
authors.affiliation: Albert Einstein College of Medicine, Center for C. elegans Anatomy, 1410 Pelham Parkway South, Room 601, Bronx, NY, 10461, USA. david.hall@einstein.yu.edu.
authors.forename: David H
authors.initials: DH
authors.lastname: Hall
authors.affiliation: New York Structural Biology Center, New York, NY, USA. rice@nysbc.org.
authors.forename: William J
authors.initials: WJ
authors.lastname: Rice
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000648:ultrastructure*; D055032:Electron Microscope Tomography / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D012984:Software
keywords: Annotation; Back-projection; Denoising; Electron tomogram; Fiducial marker; High pressure freezing; Image acquisition; Image alignment; Markerless alignment