title: Influenza and respiratory syncytial viruses: Efficacy of different diagnostic assays.
abstract: OBJECTIVE
  To determine the efficacy of cell culture, immunoflourescence Assay (IFA) and real time polymerase chain reaction (rRT-PCR) in relation to diagnosis of influenza and Respiratory Syncytial Virus (RSV).
  
  
  METHODS
  Total 2781 specimens of throat swabs and nasopharyngeal aspirates were obtained from patients suspected of respiratory viruses' infections from January 2009 to December 2011 at Universiti Kebangsaan Malaysia Medical Centre(UKMMC). The specimens were processed by cell culture and immunoflurescence assay (IFA) and (rRT-PCR).
  
  
  RESULTS
  Thirty three (1.19%) specimens were positive for influenza virus A and 42 (1.51%) were positive for RSV by cell culture and IFA. On the other hand, rRT-PCR was able to identify 189 of 505 (37.43%) specimens in which 65 were influenza A virus and 124 were RSV. Sensitivity of rRT-PCR was 100% for both influenza A virus and RSV and specificity was 88% and 77% for influenza A virus and RSV, respectively.
  
  
  CONCLUSIONS
  rRT-PCR diagnosed respiratory viruses in shorter time with a high level of sensitivity in comparison to conventional assays - cell culture and IFA. These advantages help in managing patients by saving cost and hospitalization stay.
authors.affiliation: M. M. Rahman, Department of Medical Microbiology & Immunology, Faculty of Medicine, The National University of Malaysia, Cheras, 56000, Kuala Lumpur, Malaysia.
authors.forename: M M
authors.initials: MM
authors.lastname: Rahman
authors.affiliation: K.K. Wong, Department of Medical Microbiology & Immunology, Faculty of Medicine, The National University of Malaysia, Cheras, 56000, Kuala Lumpur, Malaysia.
authors.forename: K K
authors.initials: KK
authors.lastname: Wong
authors.affiliation: H. Alfizah, Department of Medical Microbiology & Immunology, Faculty of Medicine, The National University of Malaysia, Cheras, 56000, Kuala Lumpur, Malaysia.
authors.forename: H
authors.initials: H
authors.lastname: Alfizah
authors.affiliation: S. Hussin, Department of Medical Microbiology & Immunology, Faculty of Medicine, The National University of Malaysia, Cheras, 56000, Kuala Lumpur, Malaysia.
authors.forename: S
authors.initials: S
authors.lastname: Hussin
authors.affiliation: I. Isahak, Department of Medical Sciences, Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia (USIM), 55100 Kuala Lumpur, Malaysia.
authors.forename: I
authors.initials: I
authors.lastname: Isahak
keywords: Cell culture; Immunoflurescence Assay; Influenza virus; Real-time polymerase chain reaction (rRT-PCR); Respiratory Syncytial Virus