title: Metabolomic charactetization of yeast cells after dehydration stress.
abstract: In this study, we analyzed the metabolite features of the yeasts Saccharomyces cerevisiae, Naumovia castellii, and Saccharomyces mikatae. The three species are closely related genetically but differ in their tolerance of desiccation stress. Specifically, we determined whether certain metabolites correlated with cell viability after stress imposition. The metabolomics profiles of these strains were compared before cell desiccation and after cell rehydration. In S. mikatae, the presence of lysine or glutamine during rehydration led to a 20% increase in survival whereas during dehydration the levels of both amino acids in this yeast were drastically reduced.
authors.affiliation: Department of Biochemistry and Biotechnology, University Rovira Virgili, Tarragona, Spain.
authors.forename: Gema
authors.initials: G
authors.lastname: López-Martínez
authors.affiliation: Department of Biochemistry and Biotechnology, University Rovira Virgili, Tarragona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Borrull
authors.affiliation: Department of Biochemistry and Biotechnology, University Rovira Virgili, Tarragona, Spain.
authors.forename: Montse
authors.initials: M
authors.lastname: Poblet
authors.affiliation: Department of Biochemistry and Biotechnology, University Rovira Virgili, Tarragona, Spain.
authors.forename: Nicolás Rozès
authors.initials: NR
authors.lastname: Roy
authors.affiliation: Department of Biochemistry and Biotechnology, University Rovira Virgili, Tarragona, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Cordero-Otero
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D003890:Desiccation; D050296:Microbial Viability; D012440:Saccharomyces / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013312:Stress, Physiological; D014867:Water / Q000032:analysis / Q000378:metabolism
chemical_list: D000596:Amino Acids; D014867:Water
keywords: Naumovia castellii; Saccaromyces mikatae; Saccharomyces cerevisiae; dehydration stress; metabolite extraction; viability; wild yeast