title: Thermoseparating aqueous two-phase systems: Recent trends and mechanisms.
abstract: Having the benefits of being environmentally friendly, providing a mild environment for bioseparation, and scalability, aqueous two-phase systems (ATPSs) have increasingly caught the attention of industry and researchers for their application in the isolation and recovery of bioproducts. The limitations of conventional ATPSs give rise to the development of temperature-induced ATPSs that have distinctive thermoseparating properties and easy recyclability. This review starts with a brief introduction to thermoseparating ATPSs, including its history, unique characteristics and advantages, and lastly, key factors that influence partitioning. The underlying mechanism of temperature-induced ATPSs is covered together with a summary of recent applications. Thermoseparating ATPSs have been proven as a solution to the demand for economically favorable and environmentally friendly industrial-scale bioextraction and purification techniques.
authors.affiliation: Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih, Selangor Darul Ehsan, Malaysia.
authors.forename: Yoong Kit
authors.initials: YK
authors.lastname: Leong
authors.affiliation: Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Material Science, Yuan Ze University, Chungli, Taoyuan, Taiwan.
authors.forename: John Chi-Wei
authors.initials: JC
authors.lastname: Lan
authors.affiliation: School of Biosciences, Faculty of Science, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih, Selangor Darul Ehsan, Malaysia.
authors.forename: Hwei-San
authors.initials: HS
authors.lastname: Loh
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Tau Chuan
authors.initials: TC
authors.lastname: Ling
authors.affiliation: Chemical Engineering, School of Engineering, Monash University, Bandar Sunway, Selangor, Malaysia.
authors.forename: Chien Wei
authors.initials: CW
authors.lastname: Ooi
authors.affiliation: Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih, Selangor Darul Ehsan, Malaysia.|Manufacturing and Industrial Processes Division, Faculty of Engineering, Centre for Food and Bioproduct Processing, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih, Selangor Darul Ehsan, Malaysia.
authors.forename: Pau Loke
authors.initials: PL
authors.lastname: Show
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000191:economics / Q000379:methods*; D003362:Cost-Benefit Analysis; D004247:DNA / Q000737:chemistry; D004852:Epoxy Compounds / Q000737:chemistry; D005027:Ethylene Oxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008970:Molecular Weight; D011108:Polymers / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D004852:Epoxy Compounds; D008024:Ligands; D011108:Polymers; D012492:Salts; D012996:Solutions; D014867:Water; D004247:DNA; D005027:Ethylene Oxide; C009068:propylene oxide
keywords: Aqueous two-phase systems; Bioseparation; Downstream processing; Thermoseparating polymers