title: Hyperglycemic Stress Impairs the Stemness Capacity of Kidney Stem Cells in Rats.
abstract: The incidence of acute kidney injury in patients with diabetes is significantly higher than that of patients without diabetes, and may be associated with the poor stemness capacity of kidney stem cells (KSCs) and limited recovery of injured renal tubules. To investigate the effects of hyperglycemic stress on KSC stemness, KSCs were isolated from the rat renal papilla and analyzed for their self-renewal and differentiation abilities. Our results showed that isolated KSCs expressed the mesenchymal stem cell markers N-cadherin, Nestin, CD133, CD29, CD90, and CD73. Moreover, KSCs co-cultured with hypoxia-injured renal tubular epithelial cell (RTECs) induced the expression of the mature epithelial cell marker CK18, suggesting that the KSCs could differentiate into RTECs in vitro. However, KSC proliferation, differentiation ability and tolerance to hypoxia were decreased in high-glucose cultures. Taken together, these results suggest the high-glucose microenvironment can damage the reparative ability of KSCs. It may result in a decreased of recovery capability of renal tubules from injury.
authors.affiliation: Department of Geriatric Nephrology, Chinese PLA General Hospital, State Key Laboratory of Kidney Disease, Beijing, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Department of Geriatric Endocrinology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Geriatric Nephrology, Chinese PLA General Hospital, State Key Laboratory of Kidney Disease, Beijing, China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Cheng
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Yue
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing, China.
authors.forename: Xuetao
authors.initials: X
authors.lastname: Pei
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D006943:Hyperglycemia / Q000473:pathology*; D007668:Kidney / Q000276:immunology / Q000473:pathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000276:immunology / Q000473:pathology*