title: Effect of honokiol on exotoxin proteins listeriolysin O and p60 secreted by Listeria monocytogenes.
abstract: Listeria monocytogenes is considered one of the most important foodborne pathogens. The virulence-related proteins listeriolysin O (LLO) and p60 are critical factors involved in Listeria pathogenesis. In the present study, we investigated the effect of honokiol on LLO and p60 secreted from L. monocytogenes. A listeriolysin assay was used to investigate the haemolytic activities of L. monocytogenes exposed to honokiol, and the secretion of LLO and p60 was detected by immunoblot analysis. Additionally, the influence of honokiol on the transcription of LLO and p60 genes (hly and iap, respectively) was analysed by real-time reverse transcription PCR. TNF-α release assays were performed to elucidate the biological relevance of changes in LLO and p60 secretion induced by honokiol. According to the data, honokiol showed good anti-L. monocytogenes activity, with MICs of 8-16 μg ml(-1), and the secretion of LLO and p60 was decreased by honokiol. In addition, the transcription of hly and iap was inhibited by honokiol. Our results indicate that TNF-α production by RAW264.7 cells stimulated with L. monocytogenes supernatants was inhibited by honokiol. Based on these data, we propose that honokiol could be used as a promising natural compound against L. monocytogenes and its virulence factors.
authors.affiliation: Public Health College, Jilin University, 130062 Changchun, PR China.|Jilin Enrty-exit Inspection and Quarantine Bureau, Changchun, 130062, PR China.
authors.forename: Rizeng
authors.initials: R
authors.lastname: Meng
authors.affiliation: Public Health College, Jilin University, 130062 Changchun, PR China.|Department of Food Quality and Safety, Jilin University, 130062 Changchun, PR China.
authors.forename: Ziwen
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Public Health College, Jilin University, 130062 Changchun, PR China.|Department of Food Quality and Safety, Jilin University, 130062 Changchun, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Guo
authors.affiliation: Department of Food Quality and Safety, Jilin University, 130062 Changchun, PR China.
authors.forename: Zonghui
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Food Quality and Safety, Jilin University, 130062 Changchun, PR China.
authors.forename: Xingchen
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Food Quality and Safety, Jilin University, 130062 Changchun, PR China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Food Quality and Safety, Jilin University, 130062 Changchun, PR China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Food Quality and Safety, Jilin University, 130062 Changchun, PR China.
authors.forename: Ce
authors.initials: C
authors.lastname: Shi
authors.affiliation: Jilin Enrty-exit Inspection and Quarantine Bureau, Changchun, 130062, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Nie
authors.affiliation: Jilin Enrty-exit Inspection and Quarantine Bureau, Changchun, 130062, PR China.
authors.forename: Weilin
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, 130062 Changchun, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Jilin Enrty-exit Inspection and Quarantine Bureau, Changchun, 130062, PR China.
authors.forename: Wenchen
authors.initials: W
authors.lastname: Ma
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, 130062 Changchun, PR China.|Public Health College, Jilin University, 130062 Changchun, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yu
authors.affiliation: Public Health College, Jilin University, 130062 Changchun, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D002460:Cell Line; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017705:Lignans / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001713:Biphenyl Compounds; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D017705:Lignans; D014409:Tumor Necrosis Factor-alpha; C078516:60 kDa protein, Listeria monocytogenes; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols; C038705:hlyA protein, Listeria monocytogenes