title: Antigen exposure in the late light period induces severe symptoms of food allergy in an OVA-allergic mouse model.
abstract: The mammalian circadian clock controls many physiological processes that include immune responses and allergic reactions. Several studies have investigated the circadian regulation of intestinal permeability and tight junctions known to be affected by cytokines. However, the contribution of circadian clock to food allergy symptoms remains unclear. Therefore, we investigated the role of the circadian clock in determining the severity of food allergies. We prepared an ovalbumin food allergy mouse model, and orally administered ovalbumin either late in the light or late in the dark period under light-dark cycle. The light period group showed higher allergic diarrhea and weight loss than the dark period group. The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group. Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group. We have demonstrated that increased production of type 2 cytokines and intestinal permeability in the light period induced severe food allergy symptoms. Our results suggest that the time of food antigen intake might affect the determination of the severity of food allergy symptoms.
authors.affiliation: Department of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Tanabe
authors.affiliation: Department of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Kitagawa
authors.affiliation: Department of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Misaki
authors.initials: M
authors.lastname: Wada
authors.affiliation: Department of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Haraguchi
authors.affiliation: Waseda Institute for Advanced Study, Waseda University, Tokyo, Japan.
authors.forename: Kanami
authors.initials: K
authors.lastname: Orihara
authors.affiliation: Department of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.|Waseda Institute for Advanced Study, Waseda University, Tokyo, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Tahara
authors.affiliation: Department of Immunology, Faculty of Medicine, University of Yamanashi, Yamanashi, Japan.
authors.forename: Atsuhito
authors.initials: A
authors.lastname: Nakao
authors.affiliation: Department of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Shibata
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D002463:Cell Membrane Permeability; D016207:Cytokines / Q000096:biosynthesis; D003967:Diarrhea / Q000175:diagnosis / Q000209:etiology; D004195:Disease Models, Animal; D005512:Food Hypersensitivity / Q000150:complications / Q000175:diagnosis / Q000276:immunology*; D005786:Gene Expression Regulation; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D008643:Mesentery; D051379:Mice; D062793:Occludin / Q000235:genetics / Q000378:metabolism; D010047:Ovalbumin / Q000009:adverse effects / Q000276:immunology; D017440:Photoperiod; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012720:Severity of Illness Index; D019108:Tight Junctions / Q000378:metabolism
chemical_list: D000485:Allergens; D050976:Basic-Leucine Zipper Transcription Factors; D016207:Cytokines; C497441:Nfil3 protein, mouse; D062793:Occludin; D012333:RNA, Messenger; D007073:Immunoglobulin E; D010047:Ovalbumin