title: COMPARISON BETWEEN FOUR USUAL METHODS OF IDENTIFICATION OF Candida SPECIES.
abstract: Infection by Candida spp. is associated with high mortality rates, especially when treatment is not appropriate and/or not immediate. Therefore, it is necessary to correctly identify the genus and species of Candida. The aim of this study was to compare the identification of 89 samples of Candida spp. by the manual methods germ tube test, auxanogram and chromogenic medium in relation to the ID 32C automated method. The concordances between the methods in ascending order, measured by the Kappa index were: ID 32C with CHROMagar Candida(κ = 0.38), ID 32C with auxanogram (κ = 0.59) and ID 32C with germ tube (κ = 0.9). One of the species identified in this study was C. tropicalis,which demonstrated a sensitivity of 46.2%, a specificity of 95.2%, PPV of 80%, NPV of 81.1%, and an accuracy of 80.9% in tests performed with CHROMagar Candida;and a sensitivity of 76.9%, a specificity of 96.8%, PPV of 90.9%, NPV of 91%, and an accuracy of 91% in the auxanogram tests. Therefore, it is necessary to know the advantages and limitations of methods to choose the best combination between them for a fast and correct identification of Candida species.
authors.affiliation: Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, BR.
authors.forename: Margarida Neves
authors.initials: MN
authors.lastname: Souza
authors.affiliation: Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, BR.
authors.forename: Stéfanie Otowicz
authors.initials: SO
authors.lastname: Ortiz
authors.affiliation: Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, BR.
authors.forename: Marcelo Martins
authors.initials: MM
authors.lastname: Mello
authors.affiliation: Irmandade Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, RS, BR.
authors.forename: Flávio de Mattos
authors.initials: Fde M
authors.lastname: Oliveira
authors.affiliation: Irmandade Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, RS, BR.
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: Severo
authors.affiliation: Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, BR.
authors.forename: Cristine Souza
authors.initials: CS
authors.lastname: Goebel
mesh_terms: D002175:Candida / Q000145:classification* / Q000302:isolation & purification; D003470:Culture Media; D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D003470:Culture Media