title: Cell Fate of Müller Cells During Photoreceptor Regeneration in an N-Methyl-N-nitrosourea-Induced Retinal Degeneration Model of Zebrafish.
abstract: Zebrafish can regenerate several organs such as the tail fin, heart, central nervous system, and photoreceptors. Very recently, a study has demonstrated the photoreceptor regeneration in the alkylating agent N-methyl-N-nitrosourea (MNU)-induced retinal degeneration (RD) zebrafish model, in which whole photoreceptors are lost within a week after MNU treatment and then regenerated within a month. The research has also shown massive proliferation of Müller cells within a week. To address the question of whether proliferating Müller cells are the source of regenerating photoreceptors, which remains unknown in the MNU-induced zebrafish RD model, we employed a BrdU pulse-chase technique to label the proliferating cells within a week after MNU treatment. As a result of the BrdU pulse-chase technique, a number of BrdU(+) cells were observed in the outer nuclear layer as well as the inner nuclear layer. This implies that regenerating photoreceptors are derived from proliferating Müller cells in the zebrafish MNU-induced RD model.
authors.affiliation: Wellness Promotion Science Center, Institute of Medial, Pharmaceutical and Health Sciences, Kanazawa University, 5-11-80 Kodatsuno, 920-0942, Kanazawa, Ishikawa, Japan. kazuhiro@staff.kanazawa-u.ac.jp.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ogai
authors.affiliation: Department of Clinical Laboratory Sciences, Graduate School of Medical Science, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa, 920-0942, Ishikawa, Japan. ef.ef.soylatte.ts@gmail.com.
authors.forename: Suguru
authors.initials: S
authors.lastname: Hisano
authors.affiliation: Department of Clinical Laboratory Sciences, Graduate School of Medical Science, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa, 920-0942, Ishikawa, Japan. sugitani@staff.kanazawa-u.ac.jp.
authors.forename: Kayo
authors.initials: K
authors.lastname: Sugitani
authors.affiliation: Graduate School and Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, 3500-3 Minamitamagaki, 513-8670, Suzuka, Mie, Japan. koriyama@suzuka-u.ac.jp.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Koriyama
authors.affiliation: Department of Molecular Neurobiology, Graduate School of Medical Science, Kanazawa University, 13-1 Takaramachi, Kanazawa, 920-8640, Ishikawa, Japan. satoru@med.kanazawa-u.ac.jp.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kato
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation; D004195:Disease Models, Animal; D063928:Ependymoglial Cells / Q000378:metabolism / Q000502:physiology*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D008770:Methylnitrosourea; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism / Q000502:physiology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012038:Regeneration / Q000502:physiology*; D012162:Retinal Degeneration / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D015027:Zebrafish
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D008770:Methylnitrosourea; D001973:Bromodeoxyuridine
keywords: Cell proliferation; Müller cells; N-methyl-N-nitrosourea (MNU); Photoreceptor; Regeneration; Zebrafish