title: Induction and Analysis of Synchronous Meiotic Yeast Cultures.
abstract: Meiosis in Saccharomyces cerevisiae can be induced by deprivation of nutrients. Here, we present a protocol for inducing synchronous meiosis in SK1, the most efficient and synchronous yeast strain for meiosis, by exposing SK1 cells to liquid medium that contains potassium acetate as a nonfermentable carbon source and lacks nitrogen. These synchronous meiotic yeast cultures can be subjected to a range of molecular and cytological analyses, making them useful for investigating the genetic and molecular determinants of meiosis.
authors.affiliation: Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University, Cleveland, Ohio 44115-2214;
authors.forename: G Valentin
authors.initials: GV
authors.lastname: Börner
authors.affiliation: North West Cancer Research Institute, School of Medical Sciences, Bangor University, Bangor LL57 2UW, United Kingdom.
authors.forename: Rita S
authors.initials: RS
authors.lastname: Cha
mesh_terms: D002244:Carbon / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D008540:Meiosis; D009584:Nitrogen / Q000378:metabolism*; D019347:Potassium Acetate / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D003470:Culture Media; D002244:Carbon; D019347:Potassium Acetate; D009584:Nitrogen