title: [Analysis of GHB and Its Precursors in Urine and Their Forensic Application].
abstract: OBJECTIVE
  To establish the method to analyze γ-hydroxybutyric acid (GHB) and its precursors 1,4-butanediol (1,4-BD) and gamma-butyrolactone (GBL) in urine through LC-MS/MS and provide evidence for related cases.
  
  
  METHODS
  GHB-d6 and MOR-d3 were used as the internal standard. The urine sample was separated by LC after protein precipitation with methanol. The electrospray ion source was for ionization. Each compound was detected through multiple-reaction monitoring (MRM) mode.
  
  
  RESULTS
  The limits of detection of GHB and its precursors 1,4-BD and GBL were 0.1, 0.1 and 2 μg/mL. The accuracy was 87.6%-98.1%. The intra-day and inter-day precisions were less than 15% and matrix effects were higher than 80%.
  
  
  CONCLUSIONS
  The method is high sensitive, simple, rapid, specific and with high reliability. This study has provided technical support and basic data for forensic cases involving GHB.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.forename: Xiao-pei
authors.initials: XP
authors.lastname: Cui
authors.forename: Ping
authors.initials: P
authors.lastname: Xiang
authors.forename: Bao-hua
authors.initials: BH
authors.lastname: Shen
mesh_terms: D015107:4-Butyrolactone / Q000652:urine*; D002072:Butylene Glycols / Q000652:urine*; D002853:Chromatography, Liquid; D044707:Forensic Sciences; D006801:Humans; D006885:Hydroxybutyrates / Q000652:urine*; D013058:Mass Spectrometry; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D002072:Butylene Glycols; D006885:Hydroxybutyrates; C111420:4-hydroxybutyric acid; C039681:1,4-butanediol; D015107:4-Butyrolactone