title: High reactive oxygen species levels are detected at the end of the chronological life span of translocant yeast cells.
abstract: Chromosome translocation is a major genomic event for a cell, affecting almost every of its life aspects ranging from metabolism, organelle maintenance and homeostasis to gene maintenance and expression. By using the bridge-induced translocation system, we defined the effects of induced chromosome translocation on the chronological life span (CLS) of yeast with particular interest to the oxidative stress condition. The results demonstrate that every translocant strain has a different CLS, but all have a high increase in reactive oxygen species and in lipid peroxides levels at the end of the life span. This could be due to the very unique and strong deregulation of the oxidative stress network. Furthermore, the loss of the translocated chromosome occurs at the end of the life span and is locus dependent. Additionally, the RDH54 gene may play a role in the correct segregation of the translocant chromosome, since in its absence there is an increase in loss of the bridge-induced translocated chromosome.
authors.affiliation: Yeast Molecular Genetics Group, ICGEB, Area Science Park, Padriciano 99, 34149, Trieste, Italy.|Department of Chromosome Biology, Max Perutz Laboratories, Dr. Bohr-Gasse 9, 1030, Vienna, Austria.
authors.forename: Jason
authors.initials: J
authors.lastname: Sims
authors.affiliation: Yeast Molecular Genetics Group, ICGEB, Area Science Park, Padriciano 99, 34149, Trieste, Italy.|Central European Initiative, Via Genova 9, 34121, Trieste, Italy.
authors.forename: Carlo V
authors.initials: CV
authors.lastname: Bruschi
authors.affiliation: Cellular and Molecular Life Sciences, University of Rennes, 9 Rue Jean Macé, 35700, Rennes, France.
authors.forename: Chloé
authors.initials: C
authors.lastname: Bertin
authors.affiliation: Yeast Molecular Genetics Group, ICGEB, Area Science Park, Padriciano 99, 34149, Trieste, Italy.
authors.forename: Nicole
authors.initials: N
authors.lastname: West
authors.affiliation: Genetics Division, Department of Cell Biology, University of Salzburg, Hellbrunnerstrasse 34, 5020, Salzburg, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Breitenbach
authors.affiliation: Genetics Division, Department of Cell Biology, University of Salzburg, Hellbrunnerstrasse 34, 5020, Salzburg, Austria.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Schroeder
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50, 8010, Graz, Austria.
authors.forename: Tobias
authors.initials: T
authors.lastname: Eisenberg
authors.affiliation: Genetics Division, Department of Cell Biology, University of Salzburg, Hellbrunnerstrasse 34, 5020, Salzburg, Austria.
authors.forename: Mark
authors.initials: M
authors.lastname: Rinnerthaler
authors.affiliation: Institute of Food, Nutrition and Health, University of Primorska, Polje 42, 6310, Izola, Slovenia.
authors.forename: Peter
authors.initials: P
authors.lastname: Raspor
authors.affiliation: Yeast Molecular Genetics Group, ICGEB, Area Science Park, Padriciano 99, 34149, Trieste, Italy. tosato@icgeb.org.
authors.forename: Valentina
authors.initials: V
authors.lastname: Tosato
mesh_terms: D005656:Fungal Proteins / Q000235:genetics; D008054:Lipid Peroxides / Q000378:metabolism; D008136:Longevity / Q000235:genetics*; D018384:Oxidative Stress / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014178:Translocation, Genetic / Q000235:genetics; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D008054:Lipid Peroxides; D017382:Reactive Oxygen Species
keywords: Ageing; Chromosomal translocation; Life span; Oxidative stress; Yeast