title: Studies on Immunogenicity and Antigenicity of Baculovirus-Expressed Binding Region of Plasmodium falciparum EBA-140 Merozoite Ligand.
abstract: The erythrocyte binding ligand 140 (EBA-140) is a member of the Plasmodium falciparum erythrocyte binding antigens (EBA) family, which are considered as prospective candidates for malaria vaccine development. EBA proteins were identified as important targets for naturally acquired inhibitory antibodies. Natural antibody response against EBA-140 ligand was found in individuals living in malaria-endemic areas. The EBA-140 ligand is a paralogue of the well-characterized P. falciparum EBA-175 protein. They both share homology of domain structure, including the binding region (Region II), which consists of two homologous F1 and F2 domains and is responsible for ligand-erythrocyte receptor interaction during merozoite invasion. It was shown that the erythrocyte receptor for EBA-140 ligand is glycophorin C-a minor human erythrocyte sialoglycoprotein. In studies on the immunogenicity of P. falciparum EBA ligands, the recombinant proteins are of great importance. In this report, we have demonstrated that the recombinant baculovirus-obtained EBA-140 Region II is immunogenic and antigenic. It can raise specific antibodies in rabbits, and it is recognized by natural antibodies present in sera of patients with malaria, and thus, it may be considered for inclusion in multicomponent blood-stage vaccines.
authors.affiliation: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Zerka
authors.affiliation: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Rydzak
authors.affiliation: Department of Tropical Parasitology, Institute of Maritime and Tropical Medicine in Gdynia, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Lass
authors.affiliation: Department of Tropical Parasitology, Institute of Maritime and Tropical Medicine in Gdynia, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Szostakowska
authors.affiliation: Department of Tropical and Parasitic Diseases, Institute of Maritime and Tropical Medicine in Gdynia, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Wacław
authors.initials: W
authors.lastname: Nahorski
authors.affiliation: Department of Tropical and Parasitic Diseases, Institute of Maritime and Tropical Medicine in Gdynia, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wroczyńska
authors.affiliation: Department of Tropical Parasitology, Institute of Maritime and Tropical Medicine in Gdynia, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Przemyslaw
authors.initials: P
authors.lastname: Myjak
authors.affiliation: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
authors.forename: Hubert
authors.initials: H
authors.lastname: Krotkiewski
authors.affiliation: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland. ewa.jaskiewicz@iitd.pan.wroc.pl.|Department of Molecular Biology, Faculty of Biological Sciences, University of Zielona Góra, Zielona Gora, Poland. ewa.jaskiewicz@iitd.pan.wroc.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Jaskiewicz
mesh_terms: D000818:Animals; D000917:Antibody Formation; D016367:Baculoviridae / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004912:Erythrocytes / Q000502:physiology*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D017780:Malaria Vaccines / Q000276:immunology*; D016778:Malaria, Falciparum / Q000276:immunology / Q000517:prevention & control*; D008565:Membrane Proteins; D010963:Plasmodium falciparum / Q000276:immunology*; D011485:Protein Binding; D000072417:Protein Domains / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D061987:Sf9 Cells; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D002352:Carrier Proteins; C438380:EBA140 protein, Plasmodium falciparum; D017780:Malaria Vaccines; D008565:Membrane Proteins; D015800:Protozoan Proteins; D014614:Vaccines, Synthetic
keywords: Antigenicity; Immunogenicity; Natural anti-EBA-140 IgG antibodies; Plasmodium falciparum; Recombinant binding region of EBA-140 ligand; Region II baculovirus expression