title: Nanoleakage evaluation at adhesive-dentin interfaces by different observation methods.
abstract: The purpose of this study was to evaluate the capability and characteristics of different nanoleakage observation methods, including light microscope (LM), field-emission scanning electron microscope (FESEM), transmission electron microscope (TEM), and confocal laser scanning microscope (CLSM). Dentin specimens were bonded with either an etch-and-rinse adhesive (SBMP) or a self-etch adhesive (GB), and prepared for nanoleakge evaluation according to different observation methods. LM, FESEM and CLSM results demonstrated that the SBMP group showed more interfacial nanoleakage than the GB group (p<0.05); by contrast, no significant difference was found in TEM results (p>0.05), however, TEM illustrated concrete nanoleakage forms or patterns. The results suggested that different observation methods might exhibit distinct images and a certain degree of variations in nanoleakage statistical results. Researchers should carefully design and calculate the optimum assembly in combination with qualitative and quantitative approaches to obtain objective and accurate nanoleakage evaluation.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedical Ministry of Education, School and Hospital of Stomatology, Wuhan University.
authors.forename: Hongye
authors.initials: H
authors.lastname: Yang
authors.forename: Jingmei
authors.initials: J
authors.lastname: Guo
authors.forename: Jinxin
authors.initials: J
authors.lastname: Guo
authors.forename: Hongfei
authors.initials: H
authors.lastname: Chen
authors.forename: Mirinal
authors.initials: M
authors.lastname: Somar
authors.forename: Jiaxi
authors.initials: J
authors.lastname: Yue
authors.forename: Cui
authors.initials: C
authors.lastname: Huang
mesh_terms: D001840:Dental Bonding / Q000009:adverse effects / Q000379:methods; D003763:Dental Leakage; D003804:Dentin / Q000601:surgery / Q000648:ultrastructure; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012835:Silver Nitrate
chemical_list: D017220:Dentin-Bonding Agents; D012835:Silver Nitrate