title: Reactions of superoxide dismutases with HS(-)/H2S and superoxide radical anion: An in vitro EPR study.
abstract: Interactions of hydrogen sulfide (HS(-)/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS(-)/H2S and superoxide radical anion O2.- on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS(-)/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu(2+) was reduced to Cu(1+), while manganese appears to be released from MnSOD active center. Untreated and O2.- treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe(3+) and Mn(2+). Our study provides new details on the mechanisms of (patho)physiological effects of HS(-)/H2S.
authors.affiliation: Abbott Laboratories S.A., Belgrade, Serbia.
authors.forename: Bojana
authors.initials: B
authors.lastname: Bolić
authors.affiliation: Department of Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
authors.forename: Ana
authors.initials: A
authors.lastname: Mijušković
authors.affiliation: Faculty of Physical Chemistry, University of Belgrade, Belgrade, Serbia.
authors.forename: Ana
authors.initials: A
authors.lastname: Popović-Bijelić
authors.affiliation: Department of Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Nikolić-Kokić
authors.affiliation: Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
authors.forename: Snežana
authors.initials: S
authors.lastname: Spasić
authors.affiliation: Department of Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
authors.forename: Duško
authors.initials: D
authors.lastname: Blagojević
authors.affiliation: Department of Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
authors.forename: Mihajlo B
authors.initials: MB
authors.lastname: Spasić
authors.affiliation: Department of Life Sciences, Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia. Electronic address: ivan@imsi.rs.
authors.forename: Ivan
authors.initials: I
authors.lastname: Spasojević
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D006862:Hydrogen Sulfide / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000378:metabolism*; D013481:Superoxides / Q000737:chemistry*; D000099156:Superoxide Dismutase 2
chemical_list: D006862:Hydrogen Sulfide; D013482:Superoxide Dismutase; D013481:Superoxides; D000099156:Superoxide Dismutase 2
keywords: Copper; EPR; H(2)S; Iron; Manganese; Superoxide dismutase