title: Serum nerve growth factor level indicates therapeutic efficacy of 125I seed implantation in advanced pancreatic adenocarcinoma.
abstract: OBJECTIVE
  To evaluate serum nerve growth factor (NGF) as a marker in predicting effectiveness of 125I seed implantation in advanced pancreatic carcinoma.
  
  
  METHODS
  A total of 45 patients (30 males/15 females with mean age of 52.07±8.43 years) diagnosed with advanced pancreatic adenocarcinoma (PCa) between January 2011 to May 2014 were enrolled as PCa group in this study. Tumors were categorized as at least stage III with unresectionable condition by the TNM standard. The average tumour shortest diameter was 37.54±13.84 mm (18.50-71.20 mm). NGF level in serum before 125I seed implantation and in tumor tissue resected during surgery was measured by ELISA. After treatment, CT Scan was used to serially monitor the diameters of the tumour monthly for 6-month follow-up. RECIST was applied to evaluate the efficacy. Predictive value of serum and tumour derived NGF was evaluated based on ROC curve chart.
  
  
  RESULTS
  We found that the serum NGF level was significantly increased in PCa patients (775.60 ± 250.97 pg/ml) compared to the healthy control group (35.03 ± 25.36 pg/ml), after age and gender adjustment. In the PCa group, the serum NGF level positively correlated with that from loci tumor tissue (r=0.487). The serum NGF level was compared between the effective group (537.42 ± 122.61 pg/ml) and noneffective group (883.17 ± 217.79 pg/ml), and significant difference was detected (p<0.0001). Patients with lower serum NGF level had good response to the 125I seeds implantation. Taking cut-off at 649.59 pg/ml, 85.70% specificity and 90.30% sensitivity were achieved by ROC. Area under the Curve of serum NGF was 0.945, standard deviation was 0.032, 95% confidence interval was 0.882-1.000.
  
  
  CONCLUSIONS
  The level of serum NGF could be a referential index to predict the therapeutic efficacy of 125I seed implantation treatments in advanced pancreatic adenocarcinoma.
authors.affiliation: Department of Second General Surgery, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, P.R. China. blackstone502@163.com.
authors.forename: J-B
authors.initials: JB
authors.lastname: Xu
authors.forename: X-C
authors.initials: XC
authors.lastname: Yang
authors.forename: J-H
authors.initials: JH
authors.lastname: Guo
authors.forename: J-Y
authors.initials: JY
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Shang
authors.forename: W-J
authors.initials: WJ
authors.lastname: Liu
authors.forename: T-M
authors.initials: TM
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Zou
mesh_terms: D000230:Adenocarcinoma / Q000532:radiotherapy*; D015415:Biomarkers / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020932:Nerve Growth Factor / Q000235:genetics* / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000532:radiotherapy*; D014057:Tomography, X-Ray Computed
chemical_list: D015415:Biomarkers; C502240:NGF protein, human; D020932:Nerve Growth Factor