title: Ternary Dinuclear Copper(II) Complexes of a Reduced Schiff Base Ligand with Diimine Coligands: DNA Binding, Cytotoxic Cell Apoptosis, and Apoptotic Mechanism.
abstract: A serial of mixed-ligand Cu(II) complexes of the type [Cu(phens)(H2 PDILeu)]H2 O (1-4) containing phens as 2,2'-bipyridyl (bpy, 1), 1,10-phenanthroline (phen, 2), dipyrido[3,2-d:2',3'-f]quinoxaline (dpq, 3), and dipyrido[3,2-a:2',3'-c]phenazine (dppz, 4) have been isolated and characterized. The interaction of the complexes with calf-thymus DNA has been explored by physical methods to propose modes of DNA binding of the complexes, which indicate that 4 interacts with DNA more strongly than all of the other complexes through intercalation interaction. Furthermore, cell apoptosis was detected by AnnexinV/PI flow cytometry and TUNEL assay and by Western blotting to detect the protein expression of p53, Bax, and Bcl-2. All the three copper complexes can effectively induce apoptosis of the three human tumor cells, which was accompanied with upregulation of the expression of p53 and Bax, while Bcl-2 decreased.
authors.affiliation: The Affiliated People 's Hospital of Jiangsu University, #8 Dianli Road, Zhenjiang, Jiangsu, 212000, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.affiliation: The Affiliated People 's Hospital of Jiangsu University, #8 Dianli Road, Zhenjiang, Jiangsu, 212000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: The Affiliated People 's Hospital of Jiangsu University, #8 Dianli Road, Zhenjiang, Jiangsu, 212000, China.
authors.forename: Qiaoyu
authors.initials: Q
authors.lastname: Li
authors.affiliation: The Affiliated Yixing Hospital of Jiangsu University, #75 Tongzhenguan Road, Yixing, Jiangsu, 214200, China.
authors.forename: Tieliang
authors.initials: T
authors.lastname: Ma
authors.affiliation: Department of Physics and Chemistry, Henan Polytechnic University, Jiaozuo, Henan, 454000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: The Affiliated Yixing Hospital of Jiangsu University, #75 Tongzhenguan Road, Yixing, Jiangsu, 214200, China.
authors.forename: Taofeng
authors.initials: T
authors.lastname: Zhu
authors.affiliation: The Affiliated Yixing Hospital of Jiangsu University, #75 Tongzhenguan Road, Yixing, Jiangsu, 214200, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
authors.affiliation: The Affiliated Yixing Hospital of Jiangsu University, #75 Tongzhenguan Road, Yixing, Jiangsu, 214200, China.
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Zhu
authors.affiliation: The Affiliated Yixing Hospital of Jiangsu University, #75 Tongzhenguan Road, Yixing, Jiangsu, 214200, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Department of Physics and Chemistry, Henan Polytechnic University, Jiaozuo, Henan, 454000, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Dong
authors.affiliation: Department of Physics and Chemistry, Henan Polytechnic University, Jiaozuo, Henan, 454000, China.
authors.forename: Jiancui
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Physics and Chemistry, Henan Polytechnic University, Jiaozuo, Henan, 454000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jia
mesh_terms: D000588:Amines / Q000737:chemistry*; D017209:Apoptosis; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D012545:Schiff Bases / Q000737:chemistry*
chemical_list: D000588:Amines; D008024:Ligands; D012545:Schiff Bases; D003300:Copper; D004247:DNA
keywords: DNA-binding properties; cell apoptosis; cytotoxicity; ternary Cu(II) complexes