title: Anticancer Effects of Curcuma C20-Dialdehyde against Colon and Cervical Cancer Cell Lines.
abstract: BACKGROUND
  Recent attention on chemotherapeutic intervention against cancer has been focused on discovering and developing phytochemicals as anticancer agents with improved efficacy, low drug resistance and toxicity, low cost and limited adverse side effects. In this study, we investigated the effects of Curcuma C20-dialdehyde on growth, apoptosis and cell cycle arrest in colon and cervical cancer cell lines.
  
  
  METHODS
  Antiproliferative, apoptosis induction, and cell cycle arrest activities of Curcuma C20-dialdehyde were determined by WST cell proliferation assay, flow cytometric Alexa fluor 488-annexin V/propidium iodide (PI) staining and PI staining, respectively.
  
  
  RESULTS
  Curcuma C20 dialdehyde suppressed the proliferation of HCT116, HT29 and HeLa cells, with IC50 values of 65.4±1.74 μg/ml, 58.4±5.20 μg/ml and 72.0±0.03 μg/ml, respectively, with 72 h exposure. Flow cytometric analysis revealed that percentages of early apoptotic cells increased in a dose-dependent manner upon exposure to Curcuma C20-dialdehyde. Furthermore, exposure to lower concentrations of this compound significantly induced cell cycle arrest at G1 phase for both HCT116 and HT29 cells, while higher concentrations increased sub-G1 populations. However, the concentrations used in this study could not induce cell cycle arrest but rather induced apoptotic cell death in HeLa cells.
  
  
  CONCLUSIONS
  Our findings suggest that the phytochemical Curcuma C20-dialdehyde may be a potential antineoplastic agent for colon and cervical cancer chemotherapy and/or chemoprevention. Further studies are needed to characterize the drug target or mode of action of the Curcuma C20-dialdehyde as an anticancer agent.
authors.affiliation: Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand E-mail : sthanaset@kku.ac.th.
authors.forename: Supattra
authors.initials: S
authors.lastname: Chaithongyot
authors.forename: Ali
authors.initials: A
authors.lastname: Asgar
authors.forename: Gulsiri
authors.initials: G
authors.lastname: Senawong
authors.forename: Anongnat
authors.initials: A
authors.lastname: Yowapuy
authors.forename: Eric
authors.initials: E
authors.lastname: Lattmann
authors.forename: Nison
authors.initials: N
authors.lastname: Sattayasai
authors.forename: Thanaset
authors.initials: T
authors.lastname: Senawong
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004224:Diterpenes / Q000008:administration & dosage / Q000494:pharmacology*; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D045325:HCT116 Cells; D019073:HT29 Cells; D006367:HeLa Cells; D006801:Humans; D020128:Inhibitory Concentration 50
chemical_list: C553098:2-(2-(5,5,8a-trimethyl-2-methylene-decahydronaphthalen-1-yl)ethylidene)succinaldehyde; D000970:Antineoplastic Agents; D004224:Diterpenes