title: Clinical significance of microRNA-130b in osteosarcoma and in cell growth and invasion.
abstract: OBJECTIVE
  To investigate clinical significance of microRNA-130b (miR-130b) in osteosarcoma and its role in cell growth and invasion.
  
  
  METHODS
  miR-130b expression was detected in 68 samples of surgically resected osteosarcoma and matched normal tumor-adjacent tissues by qRT-PCR. The expression of miR-130b was altered by corresponding vectors in osteosarcoma cells, and then Western blot was used to detect the expression of PPARγ. BrdU cell proliferation and Transwell assays were performed to determine cell proliferation and invasion.
  
  
  RESULTS
  The expression of miR-130b in osteosarcoma tissues was significantly higher than that in normal tumor-adjacent tissues. Its expression in patients with metastasis was significantly higher than that in those without metastases. miR-130b expression in tumor tissues was significantly associated with tumor size, clinical stage and distant metastasis. And its expression was significantly correlated with overall survival and disease free survival. miR-130b overexpression obviously repressed the expression of PPARγ, and resulted in significant increase of Saos-2 cell proliferation and invasion. On the contrast, repressing miR-130b expression with its inhibitor significantly increased PPARγ expression, and inhibited MG-63 cell proliferation and invasion.
  
  
  CONCLUSIONS
  The high-expression of miR-130b is correlated with the adverse clinicopathological features and poor prognosis in osteosarcoma. miR-130b may regulate proliferation and invasion of osteosarcoma cells by targeting PPARγ, suggesting miR-130b may play a key role in the progression of osteosarcoma.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Zhejiang University, No. 79 Qingchun Road, Hangzhou 310006, China.
authors.forename: Lie-Dao
authors.initials: LD
authors.lastname: Yu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Zhejiang University, No. 79 Qingchun Road, Hangzhou 310006, China.
authors.forename: Ri-Long
authors.initials: RL
authors.lastname: Jin
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Zhejiang University, No. 79 Qingchun Road, Hangzhou 310006, China.
authors.forename: Peng-Cheng
authors.initials: PC
authors.lastname: Gu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Zhejiang University, No. 79 Qingchun Road, Hangzhou 310006, China.
authors.forename: Zhi-Heng
authors.initials: ZH
authors.lastname: Ling
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Zhejiang University, No. 79 Qingchun Road, Hangzhou 310006, China.
authors.forename: Xiang-Jin
authors.initials: XJ
authors.lastname: Lin
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Zhejiang University, No. 79 Qingchun Road, Hangzhou 310006, China. Electronic address: dujingyu24324@163.com.
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Du
keywords: Clinical significance; Invasion; MicroRNA-130b; Osteosarcoma; Tumor growth