title: Mesenchymal Stem Cells Derived from Human Bone Marrow.
abstract: Mesenchymal stem cells are found in a number of tissues and have the potential to differentiate into a range of mesenchymal lineages. This ready availability and multipotent character means that mesenchymal stem cells have become a focus for the field of tissue engineering, particularly for the repair of bone and cartilage. This chapter describes the isolation of mesenchymal stem cells from human bone marrow tissue, as well as expansion of the cells and characterisation of their multipotency.
authors.affiliation: Musculoskeletal Regeneration, AO Research Institute Davos, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland.|Cardiff University School of Biosciences, Museum Avenue, Cardiff, UK.
authors.forename: Oliver F W
authors.initials: OF
authors.lastname: Gardner
authors.affiliation: Musculoskeletal Regeneration, AO Research Institute Davos, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland.
authors.forename: Mauro
authors.initials: M
authors.lastname: Alini
authors.affiliation: Musculoskeletal Regeneration, AO Research Institute Davos, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland. martin.stoddart@aofoundation.org.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Stoddart
mesh_terms: D050156:Adipogenesis; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism / Q000502:physiology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D020219:Chondrogenesis; D006801:Humans; D007700:Kinetics; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D010012:Osteogenesis; D010641:Phenotype; D044968:Regenerative Medicine / Q000379:methods*; D013194:Staining and Labeling; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D015415:Biomarkers
keywords: Adipogenesis; Chondrogenesis; Mesenchymal stem cell; Osteogenesis