title: Phylogenesis and Biological Characterization of a New Glucose Transporter in the Chicken (Gallus gallus), GLUT12.
abstract: In mammals, insulin-sensitive GLUTs, including GLUT4, are recruited to the plasma membrane of adipose and muscle tissues in response to insulin. The GLUT4 gene is absent from the chicken genome, and no functional insulin-sensitive GLUTs have been characterized in chicken tissues to date. A nucleotide sequence is predicted to encode a chicken GLUT12 ortholog and, interestingly, GLUT12 has been described to act as an insulin-sensitive GLUT in mammals. It encodes a 596 amino acid protein exhibiting 71% identity with human GLUT12. First, we present the results of a phylogenetic study showing the stability of this gene during evolution of vertebrates. Second, tissue distribution of chicken SLC2A12 mRNA was characterized by RT-PCR. It was predominantly expressed in skeletal muscle and heart. Protein distribution was analysed by Western blotting using an anti-human GLUT12 antibody directed against a highly conserved region (87% of identity). An immuno-reactive band of the expected size (75kDa) was detected in the same tissues. Third a physiological characterization was performed: SLC2A12 mRNA levels were significantly lowered in fed chickens subjected to insulin immuno-neutralization. Finally, recruitment of immuno-reactive GLUT12 to the muscle plasma membrane was increased following 1h of intraperitoneal insulin administration (compared to a control fasted state). Thus insulin administration elicited membrane GLUT12 recruitment. In conclusion, these results suggest that the facilitative glucose transporter protein GLUT12 could act in chicken muscle as an insulin-sensitive transporter that is qualitatively similar to GLUT4 in mammals.
authors.affiliation: UR83 Recherches Avicoles, Institut National de la Recherche Agronomique, Nouzilly, France.
authors.forename: Edouard
authors.initials: E
authors.lastname: Coudert
authors.affiliation: Bioinformatique-Genomique Comparative et Evolutive, Institut National de la Recherche Agronomique, Castanet Tolosan, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Pascal
authors.affiliation: UMR 7247 INRA-CNRS-Université de Tours-Haras Nationaux, Institut National de la Recherche Agronomique, Nouzilly, France.
authors.forename: Joëlle
authors.initials: J
authors.lastname: Dupont
authors.affiliation: UR83 Recherches Avicoles, Institut National de la Recherche Agronomique, Nouzilly, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Simon
authors.affiliation: UR83 Recherches Avicoles, Institut National de la Recherche Agronomique, Nouzilly, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Cailleau-Audouin
authors.affiliation: UR83 Recherches Avicoles, Institut National de la Recherche Agronomique, Nouzilly, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Crochet
authors.affiliation: UR83 Recherches Avicoles, Institut National de la Recherche Agronomique, Nouzilly, France.
authors.forename: Michel Jacques
authors.initials: MJ
authors.lastname: Duclos
authors.affiliation: UR83 Recherches Avicoles, Institut National de la Recherche Agronomique, Nouzilly, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Tesseraud
authors.affiliation: UR83 Recherches Avicoles, Institut National de la Recherche Agronomique, Nouzilly, France.
authors.forename: Sonia
authors.initials: S
authors.lastname: Métayer-Coustard
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics* / Q000378:metabolism; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism; D006321:Heart / Q000502:physiology; D007328:Insulin / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D014018:Tissue Distribution / Q000235:genetics
chemical_list: D051246:Glucose Transport Proteins, Facilitative; D051275:Glucose Transporter Type 4; D007328:Insulin; D012333:RNA, Messenger