title: Monoclonal antibody profiling of cell surface proteins associated with the viral biofilms on HTLV-1 transformed cells.
abstract: Human T lymphotropic virus 1 (HTLV-1) is a pathogenic retrovirus that spreads predominantly via cell-to-cell contact. Two models of cell-to-cell virus transmission are proposed: virological synapse (VS) and viral biofilms (VB). Both infectious structures can be involved in transmission and synergistically enhance HTLV-1 spread between cells. Although transmission of virus via VB has been reported, the molecular composition of VB remains poorly understood. In this study we generated new anti-VB monoclonal antibodies (MAbs) and screenedthem along with a panel of anti-human cluster of differentiation (CD) MAbs to select antigens associated with VB. Among four MAbs generated against VB, two MAbs were identified as anti-CD25 (IL-2RA). We found that antigens CD4, CD150, CD25, CD70, and CD80 were enriched in VB. We also determined that expression of viral protein Tax, a central molecule in HTLV-1 transmission, upregulates intercellular adhesion molecule 1 (ICAM-1), CD95, CD25, CD70, and CD80. Whether these antigens are essential for VB formation and HTLV-1 infection remains unknown and will be determined in further experiments.
authors.forename: A
authors.initials: A
authors.lastname: Tarasevich
authors.forename: A
authors.initials: A
authors.lastname: Filatov
authors.forename: A
authors.initials: A
authors.lastname: Pichugin
authors.forename: D
authors.initials: D
authors.lastname: Mazurov
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D018441:Biofilms; D002472:Cell Transformation, Viral; D016355:Genes, pX / Q000502:physiology; D057809:HEK293 Cells; D015490:HTLV-I Infections / Q000635:transmission*; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D006801:Humans
chemical_list: D000911:Antibodies, Monoclonal
keywords: monoclonal antibody; CD antigen; HTLV-1; biofilms.