title: Stilbenoids from Rheum undulatum Protect Hepatocytes Against Oxidative Stress Through AMPK Activation.
abstract: Oxidative stress promotes several diseases, including liver disease. We have isolated several stilbenoids from Rheum undulatum to investigate their hepatoprotective activities and mechanism. Stilbenoids from R. undulatum protects hepatocytes against arachidonic acid + iron (AA + Fe) induced oxidative stress. Pterostilbene (compound 5) shows stronger activity than the others. Trimethoxystilbenoid (compound 6) shows best activity on protection of HepG2 cells from AA + Fe-induced oxidative stress, and trans-stilbenoid (compound 7) shows weak activity. These stilbenoids suppress ROS generation in AA + Fe-treated HepG2 cells and also suppress AA + Fe-induced MMP disruption. Their protective effects on AA + Fe-induced MMP disruption were abrogated by treatment of AMP-activated protein kinase (AMPK) inhibitor, compound C or transfection of dominant negative form of AMPK. Taken together, stilbenoids from R. undulatum protect hepatocytes against AA + Fe-induced oxidative stress through AMPK activation. And the methoxy groups in the aryl groups are important for their cytoprotective activity.
authors.affiliation: Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742, Republic of Korea.
authors.forename: Guang-Zhi
authors.initials: GZ
authors.lastname: Dong
authors.affiliation: Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742, Republic of Korea.
authors.forename: Yu-Ih
authors.initials: YI
authors.lastname: Lee
authors.affiliation: Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742, Republic of Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Jeong
authors.affiliation: Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742, Republic of Korea.
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Zhao
authors.affiliation: Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742, Republic of Korea.
authors.forename: Raok
authors.initials: R
authors.lastname: Jeon
authors.affiliation: Department of Natural Medicine Resources, Semyung University, 65 Semyung-ro, Jecheon, Chungbuk, 390-711, Republic of Korea.
authors.forename: Hwa Jin
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742, Republic of Korea.
authors.forename: Jae-Ha
authors.initials: JH
authors.lastname: Ryu
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D016718:Arachidonic Acid / Q000378:metabolism; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects*; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D010766:Phosphorylation; D020011:Protective Agents / Q000494:pharmacology; D012250:Rheum; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D020011:Protective Agents; D013267:Stilbenes; C107773:pterostilbene; D016718:Arachidonic Acid; D055372:AMP-Activated Protein Kinases
keywords: AMPK; Rheum undulatum; antioxidant; hepatocyte protection; stilbenoid