title: Evaluation of matrix metalloproteinase, myeloperoxidase, and oxidative damage in mesenteric ischemia-reperfusion injury.
abstract: BACKGROUND
  In this study, we investigated the alterations of matrix metalloproteinase (MMP) and tissue inhibitors of metalloproteinases (TIMPs), acute inflammation, and oxidative damage in the circulatory system and the intestine in response to mesenteric ischemia/reperfusion (I/R).
  
  
  METHODS
  Twenty-one rats were divided randomly into the following three groups (n = 7 in each group): a sham group (CG), an ischemic group (IG), and an I/R group (I/RG). MMP-9, TIMP-1, and myeloperoxidase (MPO) were measured using the enzyme-linked immunosorbent assay method, and lipid peroxidation (quantified as thiobarbituric acid reactive substances (TBARS) content), ischemia-modified albumin, the prooxidant-antioxidant balance (PAB), and ferric-reducing antioxidant power (FRAP) were measured spectrophotometrically. The degree of intestinal injury was evaluated according to the Chiu scoring system.
  
  
  RESULTS
  A significant difference between the mean serum TIMP-1 and MMP-9 levels and the alanine transaminase activity was found among the groups. Compared with the I/RG group a significant difference in the mean tissue MMP-9, MPO, and TBARS levels in addition to the PAB and FRAP was found between the CG and IG groups. The level of MMP-9 also demonstrated a strong, positive, and valid correlation with the TBA-RS levels. A significant morphological change was observed in both the IG and the I/RG groups. The degree of intestinal injury was more severe in the I/R group and was characterized by either villous denudation or villous loss.
  
  
  CONCLUSIONS
  These results suggest that MMP-9, TIMP-1, MPO, and oxidative stress may be important in the intestinal injury development that is induced by acute mesenteric I/R in a rat model. MMP-9 overexpression may increase the extent of intestinal villous loss, particularly when MMP-9 is upregulated by the TBARS present in the intestinal injury.
authors.affiliation: Department of General Surgery, Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey akocael147@yahoo.com.
authors.forename: A
authors.initials: A
authors.lastname: Kocael
authors.affiliation: Department of Biochemistry, Istanbul Education and Research Hospital, Istanbul, Turkey.
authors.forename: B B
authors.initials: BB
authors.lastname: Inal
authors.affiliation: Department of Biochemistry, Istanbul Education and Research Hospital, Istanbul, Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Guntas
authors.affiliation: Department of Pathology, Istanbul Education and Research Hospital, Istanbul, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Kelten
authors.affiliation: Carsamba Internal Medicine Center, Istanbul, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Inal
authors.affiliation: Department of Biochemistry, Istanbul Education and Research Hospital, Istanbul, Turkey.
authors.forename: H I
authors.initials: HI
authors.lastname: Topac
authors.affiliation: Department of Biochemistry, Istanbul Education and Research Hospital, Istanbul, Turkey.
authors.forename: P
authors.initials: P
authors.lastname: Kocael
authors.affiliation: Department of General Surgery, Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Simsek
authors.affiliation: Department of General Surgery, Faculty of Medicine, Kırıkkale University Kırıkkale, Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Karaca
authors.affiliation: Department of Anesthesiology, Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Z
authors.initials: Z
authors.lastname: Salihoglu
authors.affiliation: Department of Biochemistry, Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Uzun
mesh_terms: D000818:Animals; D007413:Intestinal Mucosa / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood* / Q000378:metabolism; D008638:Mesenteric Arteries / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D018384:Oxidative Stress / Q000502:physiology; D009195:Peroxidase / Q000097:blood* / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood* / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D013152:Splanchnic Circulation / Q000502:physiology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood* / Q000378:metabolism
chemical_list: C576839:TIMP1 protein, rat; D019715:Tissue Inhibitor of Metalloproteinase-1; D009195:Peroxidase; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat
keywords: Mesenteric ischemia/reperfusion; intestinal ischemic injury; matrix metalloproteinase 9; myeloperoxidase; oxidative damage; tissue inhibitor of metalloproteinase 1