title: Development of nitric oxide catalytic coatings by conjugating 3,3-disulfodipropionic acid and 3,3-diselenodipropionic acid for improving hemocompatibility.
abstract: Nitric oxide (NO), discovered as an endothelium-derived relaxing factor, has been found to have multiple intracellular effects in vascular diseases including vasorelaxation regulation, endothelial regeneration, inhibition of leukocyte chemotaxis, and platelet activation. In the work described here, the authors have developed a NO-catalytic bioactive coating for improving hemocompatibility. The authors first prepared a dopamine and hexamethylendiamine (PDAM/HD) amine-rich adherent copolymer coating to introduce amine groups onto 316L stainless steel, followed by covalently conjugating 3,3-disulfodipropionic acid (S-S) and 3,3-diselenodipropionic acid (Se-Se), which mimic glutathione peroxidase-like catalytic production of NO. S-S and Se-Se were immobilized on the PDAM/HD surface via carbodiimide coupling chemistry. X-ray photoelectron spectroscopy analysis revealed clear S2p and Se3d signals, confirming the immobilization of S-S and Se-Se on the PDAM/HD surface. The NO release behavior of different samples was investigated. In detail, two species of thionitrites (RSNO), S-nitrosoglutathione (GSNO, endogenous NO donors) and S-nitrosoacetylpenicillamine (SNAP) were chosen as NO donors to investigate the NO catalytic properties of S-S and Se-Se modified PDAM/HD surfaces. Not only Se-Se@PDAM/HD but also S-S@PDAM/HD coatings showed the ability to continuously catalyze RSNO to generate NO in the presence of proper thiol reducing agent. For the Se-Se@PDAM/HD coating, the NO release amount and rate were greater than S-S@PDAM/HD in both GSNO and SNAP conditions. The results showed that organosulfide species possesses NO catalytic ability as well as organoselenium species. The authors demonstrated that both S-S@PDAM/HD and Se-Se@PDAM/HD coatings exhibited outstanding inhibition effect on platelet adhesion, aggregation and activation via the cyclic guanylate monophosphate signal pathway. Thus these results suggested that NO catalytic coatings based on organoselenium and organosulfide species immobilization can help to improve hemocompatibility. NO-catalytic strategies possess huge potential applications in blood-contacting devices.
authors.affiliation: Key Laboratory of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China and Institute of Biomaterials and Surface Engineering, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China and Institute of Biomaterials and Surface Engineering, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Yalong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China and Institute of Biomaterials and Surface Engineering, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China and Laboratory of Biosensing and MicroMechatronics, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Pengkai
authors.initials: P
authors.lastname: Qi
authors.affiliation: Key Laboratory of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China and Laboratory of Biosensing and MicroMechatronics, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Qiufen
authors.initials: Q
authors.lastname: Tu
authors.affiliation: Key Laboratory of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China and Institute of Biomaterials and Surface Engineering, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Zhilu
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Key Laboratory of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China and Institute of Biomaterials and Surface Engineering, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D009569:Nitric Oxide / Q000378:metabolism*; D009603:Nitroso Compounds / Q000378:metabolism; D011422:Propionates / Q000378:metabolism*; D018036:Selenium Compounds / Q000378:metabolism*; D013457:Sulfur Compounds / Q000378:metabolism*
chemical_list: C524945:3,3'-diselenodipropionic acid; D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D009603:Nitroso Compounds; D011422:Propionates; D018036:Selenium Compounds; D013457:Sulfur Compounds; D009569:Nitric Oxide