title: Photonic Crystal Hydrogel Enhanced Plasmonic Staining for Multiplexed Protein Analysis.
abstract: Plasmonic nanoparticles are commonly used as optical transducers in sensing applications. The optical signals resulting from the interaction of analytes and plamsonic nanoparticles are influenced by surrounding physical structures where the nanoparticles are located. This paper proposes inverse opal photonic crystal hydrogel as 3D structure to improve Raman signals from plasmonic staining. By hybridization of the plasmonic nanoparticles and photonic crystal, surface-enhanced Raman spectroscopy (SERS) analysis of multiplexed protein is realized. It benefits the Raman analysis by providing high-density "hot spots" in 3D and extra enhancement of local electromagnetic field at the band edge of PhC with periodic refractive index distribution. The strong interaction of light and the hybrid 3D nanostructure offers new insights into plasmonic nanoparticle applications and biosensor design.
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, P. R. China.
authors.forename: Zhongde
authors.initials: Z
authors.lastname: Mu
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, P. R. China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Electrical and Computer Engineering, Department of Mechanical Engineering, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Yin
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Electrical and Computer Engineering, Department of Mechanical Engineering, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, P. R. China.
authors.forename: Zhongze
authors.initials: Z
authors.lastname: Gu
mesh_terms: D000818:Animals; D003198:Computer Simulation; D003460:Crystallization; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D008863:Microspheres; D017785:Photons; D011506:Proteins / Q000032:analysis*; D013059:Spectrum Analysis, Raman; D013194:Staining and Labeling
chemical_list: D011506:Proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: heme proteins; hydrogels; photonic crystals; plasmonic staining; surface enhanced Raman scattering