title: Traditional Chinese Medicine: Salvia miltiorrhiza Enhances Survival Rate of Autologous Adipose Tissue Transplantation in Rabbit Model.
abstract: OBJECTIVE
  This study aimed to explore the influence of traditional Chinese medicines: Salvia miltiorrhiza on the survival of auto-transplantation of adipose tissue in a rabbit model.
  
  
  METHODS
  Minced adipose tissue harvested from the scapular region was transplanted into the dorsum of the ears of New Zealand rabbits. The experimental groups were intra-peritoneally injected with S. miltiorrhiza for a total 4 weeks. The rabbits in control group were intra-peritoneally injected with normal saline. Plasma VEGF levels were assayed at week 1, 2, 4, 6, and 8 after fat tissue auto-transplantation in the dorsum of rabbit ears. Graft samples were collected and measured at week 2, 4, and 12. Survival rates were calculated, and histologic morphology was evaluated. The expression of CD31 was detected by means of immune-histochemical staining to observe neo-vascularization of the auto-transplanted fat tissue. Perilipin was detected by means of immune-histochemical staining to observe the survival of fat cells.
  
  
  RESULTS
  At 12 weeks, the survival rates in the experimental group were statistically greater than that in the control group, respectively (p < 0.05). Plasma levels of VEGF in the experimental group at different time points were significantly higher than that in the control group (p < 0.05). Histologically, grafts in the experimental group showed better survival of adipocytes and neo-vascularization. By perilipin immuno-histochemical staining, the experimental group demonstrated better adipocyte survival.
  
  
  CONCLUSIONS
  In the rabbit model, S. miltiorrhiza can promote the neo-vascularization of adipose tissue grafts and significantly improves the survival rate of auto-transplanted adipose tissue.
  
  
  METHODS
  This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University, School of Medicine, 21st Floor, No. 1 Building, No. 639, Zhi Zao Ju Road, Shanghai, China. yuoli@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University, School of Medicine, 21st Floor, No. 1 Building, No. 639, Zhi Zao Ju Road, Shanghai, China. zhangruhong@163.com.
authors.forename: Ruhong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University, School of Medicine, 21st Floor, No. 1 Building, No. 639, Zhi Zao Ju Road, Shanghai, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University, School of Medicine, 21st Floor, No. 1 Building, No. 639, Zhi Zao Ju Road, Shanghai, China.
authors.forename: Danning
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University, School of Medicine, 21st Floor, No. 1 Building, No. 639, Zhi Zao Ju Road, Shanghai, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University, School of Medicine, 21st Floor, No. 1 Building, No. 639, Zhi Zao Ju Road, Shanghai, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University, School of Medicine, 21st Floor, No. 1 Building, No. 639, Zhi Zao Ju Road, Shanghai, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University, School of Medicine, 21st Floor, No. 1 Building, No. 639, Zhi Zao Ju Road, Shanghai, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000273:Adipose Tissue / Q000637:transplantation*; D000818:Animals; D064592:Autografts / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006085:Graft Survival / Q000187:drug effects*; D008516:Medicine, Chinese Traditional; D023421:Models, Animal; D008517:Phytotherapy; D011817:Rabbits; D027561:Salvia miltiorrhiza
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Adipose tissue; Autologous transplantation; Rabbit; Salvia miltiorrhiza; Survival rate