title: Endocrine Disruptor DDE Associated with a High-Fat Diet Enhances the Impairment of Liver Fatty Acid Composition in Rats.
abstract: The banned pesticide dichlorodiphenyltrichloroethane (DDT) and its main metabolite, p,p'-dichlorodiphenyldichloroethylene (DDE), are commonly found in the food chain and in all tissues of living organisms. DDE is associated with metabolic diseases acting as an endocrine disruptor and more recently with the obesity pandemic. This study focuses on using fatty acid analysis to relate DDE exposure and metabolic dysfunction: liver and adipose tissue (visceral and subcutaneous) composition from male Wistar rats fed a standard (STD) or high-fat (HF) diet versus the addition of DDE in water. DDE exposure increased liver levels of palmitic, stearic, oleic, trans fatty, and linoleic acids having altered the n6 and n3 pathways leading to high concentrations of arachidonic acid and DHA (C22:6 n3). The results of this study confirm the close relationship between this pesticide metabolite and hepatic lipid dysfunction, underscoring its role as an emerging target for the prevention and therapy of nonalcoholic fatty liver disease (NAFLD).
authors.affiliation: CBQF - Centro de Biotecnologia e Quı́mica Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto , Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
authors.forename: Luis M
authors.initials: LM
authors.lastname: Rodríguez-Alcalá
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Porto , Centro de Investigação Médica, 4200-450 Porto, Portugal.
authors.forename: Carla
authors.initials: C
authors.lastname: Sá
authors.affiliation: CBQF - Centro de Biotecnologia e Quı́mica Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto , Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
authors.forename: Lígia L
authors.initials: LL
authors.lastname: Pimentel
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Porto , Centro de Investigação Médica, 4200-450 Porto, Portugal.|CINTESIS-Center for Research in Health Technologies and Information Systems , 4200-450 Porto, Portugal.
authors.forename: Diogo
authors.initials: D
authors.lastname: Pestana
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Porto , Centro de Investigação Médica, 4200-450 Porto, Portugal.
authors.forename: Diana
authors.initials: D
authors.lastname: Teixeira
authors.affiliation: Chemistry Investigation Centre (CIQ), Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto , Rua do Campo Alegre, 4169-007 Porto, Portugal.|Faculty of Nutrition and Food Sciences, University of Porto , Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Faria
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Porto , Centro de Investigação Médica, 4200-450 Porto, Portugal.|CINTESIS-Center for Research in Health Technologies and Information Systems , 4200-450 Porto, Portugal.
authors.forename: Conceição
authors.initials: C
authors.lastname: Calhau
authors.affiliation: CBQF - Centro de Biotecnologia e Quı́mica Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto , Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Gomes
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D003633:Dichlorodiphenyl Dichloroethylene / Q000378:metabolism* / Q000633:toxicity; D059305:Diet, High-Fat / Q000009:adverse effects; D052244:Endocrine Disruptors / Q000378:metabolism* / Q000633:toxicity; D005227:Fatty Acids / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism*; D010575:Pesticides / Q000378:metabolism* / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar
chemical_list: D052244:Endocrine Disruptors; D005227:Fatty Acids; D010575:Pesticides; D003633:Dichlorodiphenyl Dichloroethylene
keywords: DDE; NAFLD; adipose tissue; fatty acids; liver; obesity