title: Production and Screening of Monoclonal Peptide Antibodies.
abstract: Hybridoma technology is a remarkable and indispensable tool for generating high-quality monoclonal antibodies. Hybridoma-derived monoclonal antibodies not only serve as powerful research and diagnostic reagents, but have also emerged as the most rapidly expanding class of therapeutic biologicals. In this chapter, an overview of hybridoma technology and the laboratory procedures used routinely for hybridoma production and antibody screening are presented, including characterization of peptide antibodies.
authors.affiliation: Department of Autoimmunology and Biomarkers, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark.
authors.forename: Nicole Hartwig
authors.initials: NH
authors.lastname: Trier
authors.affiliation: Department of Autoimmunology and Biomarkers, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark.
authors.forename: Anne
authors.initials: A
authors.lastname: Mortensen
authors.affiliation: Department of Autoimmunology and Biomarkers, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark.
authors.forename: Annette
authors.initials: A
authors.lastname: Schiolborg
authors.affiliation: Department of Autoimmunology and Biomarkers, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark. TFS@ssi.dk.
authors.forename: Tina
authors.initials: T
authors.lastname: Friis
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001709:Biotechnology; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D007114:Immunization; D051379:Mice; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D010455:Peptides
keywords: Enzyme-linked immunosorbent assay; Fusion; HybER; Hybridoma enhancing reagent; Immunization; Isotype determination; Monoclonal antibody; Peptides; Screening; Selection