title: TAL Effector DNA-Binding Principles and Specificity.
abstract: Transcription activator-like effectors (TALEs) are proteins with a unique DNA-binding domain that confers both a predictable and programmable specificity. The DNA-binding domain consists typically of 34-amino acid near-identical repeats. The repeats form a right-handed superhelical structure that wraps around the DNA double helix and exposes the variable amino acids at position 13 of each repeat to the sense strand DNA bases. Each repeat binds one base in a highly specific, non-overlapping, and comma-free fashion. Although TALE specificities are encoded in a simple way, sophisticated rules can be taken into account to build highly efficient DNA-binding modules for biotechnological use.
authors.affiliation: Department of Genetics, Martin Luther University Halle-Wittenberg, Weinbergweg 10, Halle (Saale), 06120, Germany.
authors.forename: Annekatrin
authors.initials: A
authors.lastname: Richter
authors.affiliation: Department of Genetics, Martin Luther University Halle-Wittenberg, Weinbergweg 10, Halle (Saale), 06120, Germany.
authors.forename: Jana
authors.initials: J
authors.lastname: Streubel
authors.affiliation: Department of Genetics, Martin Luther University Halle-Wittenberg, Weinbergweg 10, Halle (Saale), 06120, Germany. jens.boch@genetik.uni-halle.de.
authors.forename: Jens
authors.initials: J
authors.lastname: Boch
mesh_terms: D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D020449:Repetitive Sequences, Amino Acid / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D004247:DNA
keywords: CRISPR; Genome editing; Genome engineering; TALE; TALEN; Xanthomonas