title: CD4(+) T cell surface alpha enolase is lower in older adults.
abstract: To identify novel cell ageing markers in order to gain insight into ageing mechanisms, we adopted membrane enrichment and comparison of the CD4(+) T cell membrane proteome (purified by cell surface labelling using Sulfo-NHS-SS-Biotin reagent) between healthy young (n=9, 20-25 years) and older (n=10; 50-70 years) male adults. Following two-dimensional gel electrophoresis (2DE) to separate pooled membrane proteins in triplicates, the identity of protein spots with age-dependent differences (p<0.05 and >1.4 fold difference) was determined using liquid chromatography-mass spectrometry (LC-MS/MS). Seventeen protein spot density differences (ten increased and seven decreased in the older adult group) were observed between young and older adults. From spot intensity analysis, CD4(+) T cell surface α-enolase was decreased in expression by 1.5 fold in the older age group; this was verified by flow cytometry (n=22) and qPCR with significantly lower expression of cellular α-enolase mRNA and protein compared to young adult CD4(+) T cells (p<0.05). In an independent age-matched case-control study, lower CD4(+) T cell surface α-enolase expression was observed in age-matched patients with cardiovascular disease (p<0.05). An immune-modulatory role has been proposed for surface α-enolase and our findings of decreased expression suggest that deficits in surface α-enolase merit investigation in the context of immune dysfunction during ageing and vascular disease.
authors.affiliation: Life and Health Sciences, Aston Research Centre for Healthy Ageing, Aston University, Birmingham B4 7ET, UK.
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Bennett
authors.affiliation: Life and Health Sciences, Aston Research Centre for Healthy Ageing, Aston University, Birmingham B4 7ET, UK.
authors.forename: Edyta M
authors.initials: EM
authors.lastname: Augustyniak
authors.affiliation: Life and Health Sciences, Aston Research Centre for Healthy Ageing, Aston University, Birmingham B4 7ET, UK.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Dunston
authors.affiliation: Centre for Cardiovascular Sciences, City Hospital, Birmingham, UK.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Brown
authors.affiliation: Centre for Cardiovascular Sciences, City Hospital, Birmingham, UK.
authors.forename: Eduard
authors.initials: E
authors.lastname: Shantsila
authors.affiliation: Centre for Cardiovascular Sciences, City Hospital, Birmingham, UK.
authors.forename: Gregory Y H
authors.initials: GY
authors.lastname: Lip
authors.affiliation: Life and Health Sciences, Aston Research Centre for Healthy Ageing, Aston University, Birmingham B4 7ET, UK.
authors.forename: Rita D C
authors.initials: RD
authors.lastname: Torrao
authors.affiliation: Life and Health Sciences, Aston Research Centre for Healthy Ageing, Aston University, Birmingham B4 7ET, UK.
authors.forename: Chathyan
authors.initials: C
authors.lastname: Pararasa
authors.affiliation: Life and Health Sciences, Aston Research Centre for Healthy Ageing, Aston University, Birmingham B4 7ET, UK.
authors.forename: Ali H
authors.initials: AH
authors.lastname: Remtulla
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR UPMC CNRS 8256, Biological adaptation and ageing-IBPS, INSERM U1164, F-75005 Paris, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Ladouce
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR UPMC CNRS 8256, Biological adaptation and ageing-IBPS, INSERM U1164, F-75005 Paris, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Friguet
authors.affiliation: Life and Health Sciences, Aston Research Centre for Healthy Ageing, Aston University, Birmingham B4 7ET, UK. Electronic address: H.R.Griffiths@aston.ac.uk.
authors.forename: Helen R
authors.initials: HR
authors.lastname: Griffiths
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000201:enzymology / Q000276:immunology*; D002462:Cell Membrane / Q000201:enzymology / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010751:Phosphopyruvate Hydratase / Q000097:blood / Q000276:immunology*
chemical_list: D010751:Phosphopyruvate Hydratase
keywords: 2D gel electrophoresis; CD4(+)T cell; Enolase; Phenotype; Plasma membrane proteomics