title: Brevibacterium metallicus sp. nov., an endophytic bacterium isolated from roots of Prosopis laegivata grown at the edge of a mine tailing in Mexico.
abstract: A Gram-positive, aerobic, nonmotile strain, NM2E3(T) was identified as Brevibacterium based on the 16S rRNA gene sequence analysis and had the highest similarities to Brevibacterium jeotgali SJ5-8(T) (97.3 %). This novel bacterium was isolated from root tissue of Prosopis laegivata grown at the edge of a mine tailing in San Luis Potosí, Mexico. Its cells were non-spore-forming rods, showing catalase and oxidase activities and were able to grow in LB medium added with 40 mM Cu(2+), 72 mM As(5+) and various other toxic elements. Anteiso-C15:0 (41.6 %), anteiso-C17:0 (30 %) and iso-C15:0 (9.5 %) were the major fatty acids. MK-8(H2) (88.4 %) and MK-7(H2) (11.6 %) were the major menaquinones. The DNA G + C content of the strain NM2E3(T) was 70.8 mol % (Tm). DNA-DNA hybridization showed that the strain NM2E3(T) had 39.8, 21.7 and 20.3 % relatedness with B. yomogidense JCM 17779(T), B. jeotgali JCM 18571(T) and B. salitolerans TRM 45(T), respectively. Based on the phenotypic and genotypic analyses, the strain NM2E3(T) (=CCBAU 101093(T) = HAMBI 3627(T) = LMG 8673(T)) is reported as a novel species of the genus Brevibacterium, for which the name Brevibacterium metallicus sp. nov., is proposed.
authors.affiliation: Departamento de Microbiología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, México, D. F., 11340, Mexico.
authors.forename: Brenda
authors.initials: B
authors.lastname: Román-Ponce
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Yong Hua
authors.initials: YH
authors.lastname: Li
authors.affiliation: Departamento de Microbiología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, México, D. F., 11340, Mexico.
authors.forename: María Soledad
authors.initials: MS
authors.lastname: Vásquez-Murrieta
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Xin Hua
authors.initials: XH
authors.lastname: Sui
authors.affiliation: Departamento de Microbiología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, México, D. F., 11340, Mexico.
authors.forename: Wen Feng
authors.initials: WF
authors.lastname: Chen
authors.affiliation: Departamento de Microbiología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, México, D. F., 11340, Mexico.
authors.forename: Paulina
authors.initials: P
authors.lastname: Estrada-de Los Santos
authors.affiliation: Departamento de Microbiología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, México, D. F., 11340, Mexico. ewang@encb.ipn.mx.|State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. ewang@encb.ipn.mx.
authors.forename: En Tao
authors.initials: ET
authors.lastname: Wang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition / Q000235:genetics; D001951:Brevibacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019216:Metals, Heavy / Q000378:metabolism*; D008800:Mexico; D008906:Mining; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D029862:Prosopis / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013559:Symbiosis / Q000502:physiology*; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D019216:Metals, Heavy; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2
keywords: Brevibacterium metallicus; Heavy metals; Prosopis laegivata; Root tissue