title: Proteomics discovery of radioresistant cancer biomarkers for radiotherapy.
abstract: Radiotherapy (RT) is one of the most important strategies in cancer treatment. Radioresistance is a major challenge to RT and results in locoregional recurrence and metastasis. Thus, there is a great interest in investigating biomarkers to distinguish radiosensitive from radioresistant (RR) cancer patients. The development of proteomic techniques has sparked new searches for novel proteins for cancer biomarker discovery. Modern proteomic techniques allow for a high-throughput analysis of samples with the visualization and quantification of thousands of potential protein and peptide markers. The discovery of RR biomarkers can provide a clue for predicting RT response and discover therapeutic targets for developing personalised medicine of individual patients. In the past decade, emerging advanced proteomic technologies have been performed to identify radiation-related biomarkers in human cancers. This review discusses the mass spectrometry (MS)-based proteomic techniques in RR cancer biomarker discovery, summarises RR biomarkers identified in cancers from proteomics-based findings and explores potential values of RR biomarkers for future clinical trials.
authors.affiliation: Cancer Care Centre, St George Hospital, Kogarah, NSW 2217, Australia; St George and Sutherland Clinical School, Faculty of Medicine, UNSW, Kensington, NSW 2052, Australia.
authors.forename: Lei
authors.initials: L
authors.lastname: Chang
authors.affiliation: Cancer Care Centre, St George Hospital, Kogarah, NSW 2217, Australia; St George and Sutherland Clinical School, Faculty of Medicine, UNSW, Kensington, NSW 2052, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Graham
authors.affiliation: Cancer Care Centre, St George Hospital, Kogarah, NSW 2217, Australia; St George and Sutherland Clinical School, Faculty of Medicine, UNSW, Kensington, NSW 2052, Australia.
authors.forename: Jingli
authors.initials: J
authors.lastname: Hao
authors.affiliation: Cancer Care Centre, St George Hospital, Kogarah, NSW 2217, Australia; St George and Sutherland Clinical School, Faculty of Medicine, UNSW, Kensington, NSW 2052, Australia.
authors.forename: Joseph
authors.initials: J
authors.lastname: Bucci
authors.affiliation: Department of Urology, St George Hospital, Kogarah, NSW 2217, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Malouf
authors.affiliation: Department of Urology, St George Hospital, Kogarah, NSW 2217, Australia; Australian School of Advanced Medicine, Macquarie University, NSW 2109, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Gillatt
authors.affiliation: Cancer Care Centre, St George Hospital, Kogarah, NSW 2217, Australia; St George and Sutherland Clinical School, Faculty of Medicine, UNSW, Kensington, NSW 2052, Australia. Electronic address: y.li@unsw.edu.au.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009369:Neoplasms / Q000532:radiotherapy*; D040901:Proteomics / Q000379:methods*; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor
keywords: Biomarker; Cancer; Mass spectrometry; Proteomics; Radioresistance; Radiotherapy