title: Gatekeepers Controlling GPCR Export and Function.
abstract: Regulated export of G protein-coupled receptors (GPCRs) from intracellular stores involves chaperones and escort proteins, which promote their progression to the cell surface, and gatekeepers, which retain them in intracellular compartments. Functional γ-aminobutyric acid (GABA)B receptors, the paradigm of this phenomenon, comprise GB1 and GB2 subunits forming a heterodimer. GB1 is retained in the endoplasmic reticulum (ER) in the absence of GB2. A specific ER-resident gatekeeper, prenylated Rab acceptor family 2 (PRAF2), is involved in GB1 retention and prevents its progression into the biosynthetic pathway. GB1 can be released from PRAF2 only on competitive interaction with GB2. PRAF2 is ubiquitous and belongs to a subgroup of the mammalian Ypt-interacting protein (Yip) family. Several other GPCRs are likely to be regulated by Yip proteins, which might be involved in the pathophysiology of human diseases that are associated with impaired receptor targeting to the cell surface.
authors.affiliation: Institut Cochin, INSERM, CNRS, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Doly
authors.affiliation: Institut Cochin, INSERM, CNRS, Université Paris Descartes, Sorbonne Paris Cité, Paris, France. Electronic address: stefano.marullo@inserm.fr.
authors.forename: Stefano
authors.initials: S
authors.lastname: Marullo
mesh_terms: D000818:Animals; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D021381:Protein Transport; D018080:Receptors, GABA-B / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D018080:Receptors, GABA-B; D033921:Vesicular Transport Proteins; D019204:GTP-Binding Proteins
keywords: G protein-coupled receptors; GABA(B) receptor; endoplasmic reticulum gatekeeper; prenylated Rab acceptor family 2; regulated export; γ-aminobutyric acid