title: Online post-column solvent assisted and direct solvent-assisted electrospray ionization for chiral analysis of propranolol enantiomers in plasma samples.
abstract: An Online post-column solvent-assisted ionization (OPSAI) method was developed for enhancing the ionization of the beta-blocker propranolol utilizing normal phase LC-MS/MS. Solvent-assisted electrospray ionization (SAESI) was studied by the introduction of the assistant solvents A: 0.5% Formic acid in Isopropanolol, B: 0.5% Formic acid in Isopropanolol-Water (1:1), and C: 0.5% Formic acid in water into the electrospray ionization chamber using a spray needle. Analyte molecules can be directly ionized by the aid of the assistant solvent spray. Both methods were applied to the chiral separation of propranolol enantiomers using normal phase analysis on cellulose-based chiral column. Interestingly, both methods are easy to handle and offer a wide range of assistant solvents that can be used in order to gain the optimum ionization of the analyte molecules. The both methods considerably improved the analyte signal and the peak area greatly increased. The propranolol average signal-to-noise (S/N) ratio was enhanced from 26±1 and 42±1 to 2341±61 and 1725±29 for R-propranolol and S-propranolol, respectively, when the post-column solvent method (OPSAI) was used with isopropanol-assistant solvent (A). While in case of solvent-assisted electrospray ionization method (SAESI) signal was enhanced from 26±1 and 42±1 to 2223±72 and 2155±58 for R-propranolol and S-propranolol, respectively, with water as an assistant solvent. The limit of detection was 10ng/mL and the method was linear in the range 50-2000ng/mL. The NPLC-MS method was applied for the determination of propranolol enantiomers in human plasma after microextraction by packed C18 sorbent.
authors.affiliation: Department of Environmental Science and Analytical Chemistry, Stockholm University, SE10691, Stockholm, Sweden; Pharmaceutical Analysis Department, Faculty of Pharmacy, Damanhour University, Damanhour, 22511, Egypt.
authors.forename: Hatem
authors.initials: H
authors.lastname: Elmongy
authors.affiliation: Pharmaceutical Analysis Department, Faculty of Pharmacy, Damanhour University, Damanhour, 22511, Egypt.
authors.forename: Hytham
authors.initials: H
authors.lastname: Ahmed
authors.affiliation: Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Alexandria, Alexandria, 21521, Egypt.
authors.forename: Abdel-Aziz
authors.initials: AA
authors.lastname: Wahbi
authors.affiliation: Department of Respiratory Medicine, Gävle Hospital and Centre for Research at Uppsala University/County Council of Gävleborg, Gävle, Sweden.
authors.forename: Hirsh
authors.initials: H
authors.lastname: Koyi
authors.affiliation: Department of Environmental Science and Analytical Chemistry, Stockholm University, SE10691, Stockholm, Sweden. Electronic address: Mohamed.astra@gmail.com.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdel-Rehim
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000097:blood* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D011433:Propranolol / Q000097:blood* / Q000737:chemistry; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000319:Adrenergic beta-Antagonists; D012997:Solvents; D011433:Propranolol
keywords: Chiral separation; Electrospray ionization; Normal phase LC; Propranolol Enantiomers; Tandem mass spectrometry