title: Seed priming with polyethylene glycol regulating the physiological and molecular mechanism in rice (Oryza sativa L.) under nano-ZnO stress.
abstract: The present study was designed to highlight the impact of seed priming with polyethylene glycol on physiological and molecular mechanism of two cultivars of Oryza sativa L. under different levels of zinc oxide nanorods (0, 250, 500 and 750 mg L(-1)). Plant growth parameters were significantly increased in seed priming with 30% PEG under nano-ZnO stress in both cultivars. Whereas, this increase was more prominent in cultivar Qian You No. 1 as compared to cultivar Zhu Liang You 06. Significant increase in photosynthetic pigment with PEG priming under stress. Antioxidant enzymes activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) as well as malondialdehyde (MDA) contents were significantly reduced with PEG priming under nano-ZnO stress. Gene expression analysis also suggested that expression of APXa, APXb, CATa, CATb, CATc, SOD1, SOD2 and SOD3 genes were down regulated with PEG priming as compared to non-primed seeds under stress. The ultrastructural analysis showed that leaf mesophyll and root cells were significantly damaged under nano-ZnO stress in both cultivars but the damage was prominent in Zhu Liang You 06. However, seed priming with PEG significantly alleviate the toxic effects of nano-ZnO stress and improved the cell structures of leaf and roots in both cultivars.
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.|Department of Agronomy, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt.
authors.forename: Sheteiwy Mohamed
authors.initials: SM
authors.lastname: Salah
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Guan
authors.initials: G
authors.lastname: Yajing
authors.affiliation: Zhejiang Nongke Seed Industry Limited Company, Hangzhou, 310021, China.
authors.forename: Cao
authors.initials: C
authors.lastname: Dongdong
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jie
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.|Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University Multan, 60000 Pakistan.
authors.forename: Nawaz
authors.initials: N
authors.lastname: Aamir
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Qijuan
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Weimin
authors.affiliation: National Agricultural Technology Extension Service Center, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Mingyu
authors.affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Jin
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D012275:Oryza / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D018384:Oxidative Stress; D010788:Photosynthesis / Q000187:drug effects; D010860:Pigments, Biological; D011092:Polyethylene Glycols / Q000494:pharmacology*; D036226:Seedlings / Q000187:drug effects; D013312:Stress, Physiological; D015034:Zinc Oxide / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D010860:Pigments, Biological; D011092:Polyethylene Glycols; D008315:Malondialdehyde; D015034:Zinc Oxide