title: Self-Assembly of Ferritin Nanoparticles into an Enzyme Nanocomposite with Tunable Size for Ultrasensitive Immunoassay.
abstract: The self-assembly of nanoparticles into larger superstructures is a powerful strategy to develop novel functional nanomaterials, as these superstructures display collective properties that are different to those displayed by individual nanoparticles or bulk samples. However, there are increasing bottlenecks in terms of size control and multifunctionalization of nanoparticle assemblies. In this study, we developed a self-assembly strategy for construction of multifunctional nanoparticle assemblies of tunable size, through rational regulation of the number of self-assembling interaction sites on each nanoparticle. As proof-of-principle, a size-controlled enzyme nanocomposite (ENC) was constructed by self-assembly of streptavidin-labeled horseradish peroxidase (SA-HRP) and autobiotinylated ferritin nanoparticles (bFNP). Our ENC integrates a large number of enzyme molecules, together with a streptavidin-coated surface, allowing for a drastic increase in enzymatic signal when the SA is bound to a biotinylated target molecule. As result, a 10 000-fold increase in sensitivity over conventional enzyme-linked immunosorbent assays (ELISA) methods was achieved in a cardiac troponin immunoassay. Our method presented here should provide a feasible approach for constructing elaborate multifunctional superstructures of tunable size useful for a broad range of biomedical applications.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences , Wuhan 430071, China.|Nursing College, Henan University , Kaifeng 475004, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Men
authors.affiliation: Nursing College, Henan University , Kaifeng 475004, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agriculture University , Wuhan 430070, China.
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences , Wuhan 430071, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences , Wuhan 430071, China.
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Zhang
authors.affiliation: Medical College, Henan University , Kaifeng 475004, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Shi
authors.affiliation: Clinical Laboratory, Kaifeng Central Hospital , Kaifeng 475001, China.
authors.forename: Jin-Li
authors.initials: JL
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences , Wuhan 430071, China.
authors.forename: Zong-Qiang
authors.initials: ZQ
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences , Wuhan 430071, China.
authors.forename: Jiao-Yu
authors.initials: JY
authors.lastname: Deng
authors.affiliation: National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101, China.
authors.forename: Dian-Bing
authors.initials: DB
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101, China.
authors.forename: Xian-En
authors.initials: XE
authors.lastname: Zhang
mesh_terms: D019921:Biotinylation; D004797:Enzyme-Linked Immunosorbent Assay; D005293:Ferritins / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D011994:Recombinant Proteins / Q000737:chemistry; D013194:Staining and Labeling; D019809:Streptavidin / Q000378:metabolism; D019210:Troponin I / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D019210:Troponin I; D005293:Ferritins; D019809:Streptavidin; D006735:Horseradish Peroxidase
keywords: enzyme nanocomposite; nanoparticles; self-assembly; ultrasensitive immunoassay