title: Toxicity of Tributyltin in Juvenile Common Carp (Cyprinus Carpio): Physiological Responses, Hepatic Gene Expression, and Stress Protein Profiling.
abstract: In this study, the effects of tributyltin (TBT) on biochemical parameters (antioxidant responses and Na(+) -K(+) -ATPase) in different tissues were investigated by using juvenile common carp (Cyprinus Carpio) as well as growth and ion regulation-related genes expression and stress-related proteins profiling in fish liver. Oxidative stress indices and Na(+) -K(+) -ATPase showed tissues-specific responses in fish exposed to different TBT concentrations. All tested genes related to GH/IGF-I axis and ion-regulation were significantly induced in the TBT group with lower concentrations (except for the igfbp3 in 10 μg/L) and were inhibited in 20 μg/L. In addition, the profiling of two proteins Hsp 70 and MT were increasing in a dose-dependent manner under TBT stress. In short, TBT-induced biochemical and molecular responses in different tissues were reflected in the measured parameters in the test. On the basis of TBT residue levels in the natural environment, more long-term experiments at lower concentrations will be necessary in the future.
authors.affiliation: Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery SciencesChinese Academy of Fishery Sciences, Wuhan, 430223, People's Republic of China.|University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zátiší 728/II, 389 25, Vodňany, Czech Republic.
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Li
authors.affiliation: Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery SciencesChinese Academy of Fishery Sciences, Wuhan, 430223, People's Republic of China.
authors.forename: Li-Qiao
authors.initials: LQ
authors.lastname: Zhong
authors.affiliation: Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery SciencesChinese Academy of Fishery Sciences, Wuhan, 430223, People's Republic of China.|College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.
authors.forename: Wei-Na
authors.initials: WN
authors.lastname: Mu
authors.affiliation: Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery SciencesChinese Academy of Fishery Sciences, Wuhan, 430223, People's Republic of China.|Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing, 400715, People's Republic of China.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Wu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002347:Carps / Q000254:growth & development; D005786:Gene Expression Regulation / Q000187:drug effects; D013006:Growth Hormone / Q000096:biosynthesis; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D006360:Heat-Shock Proteins; D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Common Carp; Gene Expression; Physiological Responses; Protein Profiling; Tributyltin