title: Osteogenic differentiation of adipose derived stem cells on high and low aspect ratio micropatterns.
abstract: Adipose derived stem cells (ADSCs) were cultured on collagen-silk fibroin films with microchannel and micropillar patterns to investigate the effects of cell morphology changes on osteogenic differentiation. Channel and pillar micropatterned films were prepared from collagen type I and silk fibroin. While higher ADSC proliferation profiles were obtained on micropillar blend film, microchannel blend films, however, caused twice higher aspect ratio and effective orientation of cells. Alkaline phosphatase activity of ADSCs was several times higher on microchannel surface when the measured activities were normalized to cell number. Effective deposition of collagen type I and mineral by the cells were observed for patterned and unpatterned films, and these extracellular matrix components were oriented along the axis of the microchannels. In conclusion, the use of collagen-fibroin blend film with microchannel topography increased the aspect ratio and alignment of cells significantly, and was also effective in the differentiation of ADSCs into osteogenic lineage.
authors.affiliation: a Department of Biotechnology , METU , Ankara 06800 , Turkey.|b BIOMATEN , METU Center of Excellence in Biomaterials and Tissue Engineering , Ankara 06800 , Turkey.
authors.forename: Esen
authors.initials: E
authors.lastname: Sayin
authors.affiliation: b BIOMATEN , METU Center of Excellence in Biomaterials and Tissue Engineering , Ankara 06800 , Turkey.
authors.forename: Erkan Türker
authors.initials: ET
authors.lastname: Baran
authors.affiliation: a Department of Biotechnology , METU , Ankara 06800 , Turkey.|b BIOMATEN , METU Center of Excellence in Biomaterials and Tissue Engineering , Ankara 06800 , Turkey.
authors.forename: Vasif
authors.initials: V
authors.lastname: Hasirci
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D000818:Animals; D012831:Bombyx; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D024042:Collagen Type I / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism; D005349:Fibroins / Q000737:chemistry; D010012:Osteogenesis / Q000502:physiology*; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D013499:Surface Properties; D013623:Tail; D046509:Tissue Culture Techniques / Q000295:instrumentation* / Q000379:methods
chemical_list: D024042:Collagen Type I; D005349:Fibroins
keywords: adipose derived stem cells; collagen; micropattern; osteogenesis; silk fibroin