title: A water soluble β-glucan of an edible mushroom Termitomyces heimii: Structural and biological investigation.
abstract: A water soluble β-glucan (PS-I) with an average molecular weight ∼ 1.48 × 10(5)Da was isolated from the alkaline extract of an edible mushroom Termitomyces heimii. PS-I contained (1 → 3)-, (1 → 6)-, (1 → 3, 6)-linked and terminal β-d-glucopyranosyl moieties in a ratio of nearly 2:1:1:1. Based on the total hydrolysis, methylation analysis, periodate oxidation, Smith degradation, partial hydrolysis and 1D/2D NMR experiments the structure of the PS-I was elucidated. On the basis of these experiments, the repeating unit of the polysaccharide was found to consist of a backbone chain of two (1 → 6)-β-D-glucopyranosyl residues, one of which was branched at O-3 position with the side chain consisting of two (1 → 3)-β-D-glucopyranosyl and a terminal β-D-glucopyranosyl residue. Cytotoxic effect of PS-I on human blood lymphocytes at varied concentrations was studied. Moreover, it also exhibited potent antioxidant activities by diminishing the ROS and NO in the nicotine stimulated lymphocytes up to 200 μg/ml.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Dilip K
authors.initials: DK
authors.lastname: Manna
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Nandi
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Manabendra
authors.initials: M
authors.lastname: Pattanayak
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Maity
authors.affiliation: Department of Human Physiology with Community Health, Vidyasagar University, Midnapore 721 102, West Bengal, India.
authors.forename: Satyajit
authors.initials: S
authors.lastname: Tripathy
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Amit K
authors.initials: AK
authors.lastname: Mandal
authors.affiliation: Department of Human Physiology with Community Health, Vidyasagar University, Midnapore 721 102, West Bengal, India.
authors.forename: Somenath
authors.initials: S
authors.lastname: Roy
authors.affiliation: Division of Plant Pathology and Microbiology, Regional Plant Resource Centre, Bhubaneswar 751 015, Odisha, India.
authors.forename: Sushri S
authors.initials: SS
authors.lastname: Tripathy
authors.affiliation: Division of Plant Pathology and Microbiology, Regional Plant Resource Centre, Bhubaneswar 751 015, Odisha, India.
authors.forename: Nibha
authors.initials: N
authors.lastname: Gupta
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India. Electronic address: sirajul_1999@yahoo.com.
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D002240:Carbohydrate Sequence; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008969:Molecular Sequence Data; D009538:Nicotine / Q000633:toxicity; D012995:Solubility; D014867:Water / Q000737:chemistry*; D047071:beta-Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000975:Antioxidants; D047071:beta-Glucans; D014867:Water; D009538:Nicotine
keywords: Biological properties; NMR studies; Termitomyces heimii; β-Glucan