title: [The frequency of peripheral blood CD14(+)HLA-DR(-/low) MDSCs is negatively correlated with the inflammation in patients with chronic hepatitis B].
abstract: OBJECTIVE
  To study the frequency of CD14⁺HLA-DR(-/low) myeloid-derived suppressor cells (MDSCs) in the peripheral blood of chronic hepatitis B (CHB) patients and the relationship with biochemical characteristics, viral load and liver pathology.
  
  
  METHODS
  The frequency of CD14⁺HLA-DR(-/low) MDSCs in the peripheral blood of 96 patients with CHB and 20 healthy control cases were detected by flow cytometry. Ultrasound-guided liver biopsies as well as HBV-related serological tests were performed in HBV-infected individuals to analyze the biochemical characteristics, viral load and pathology. The data were assessed using Spearman correlation analysis.
  
  
  RESULTS
  The frequency of the peripheral blood CD14⁺HLA-DR(-/low) MDSCs in the 96 CHB cases was (6.03 ± 0.09)%, which was significantly higher than that of the 20 healthy control cases (1.87 ± 0.05)%. The group of HBeAg positive cases had a significantly higher frequency of the peripheral blood CD14⁺HLA-DR(-/low) MDSCs compared with the group of HBeAg negative cases and the healthy control group. The frequency of CD14⁺HLA-DR(-/low) MDSCs in the peripheral blood was negatively correlated with serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. There was no correlation between the frequency of peripheral blood CD14⁺HLA-DR(-/low) MDSCs and HBV load. The frequency of CD14⁺HLA-DR(-/low) MDSCs in the peripheral blood was negatively correlated with the liver inflammation grade, but not related with the fibrosis stage in patients with CHB.
  
  
  CONCLUSIONS
  The frequency of CD14⁺HLA-DR(-/low) MDSCs is negatively correlated with the inflammation of CHB.
authors.affiliation: Department of Laboratory Medicine, Second Affiliated Hospital, Anhui Medical University, Hefei 230601, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, Second Affiliated Hospital, Anhui Medical University, Hefei 230601, China.
authors.forename: Shihe
authors.initials: S
authors.lastname: Guan
authors.affiliation: Department of Laboratory Medicine, Second Affiliated Hospital, Anhui Medical University, Hefei 230601, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of Liver Diseases, Second Affiliated Hospital, Anhui Medical University, Hefei 230601, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ye
authors.affiliation: Department of Hematology, Second Affiliated Hospital, Anhui Medical University, Hefei 230601, China.
authors.forename: Kaili
authors.initials: K
authors.lastname: Yan
authors.affiliation: Department of Laboratory Medicine, Second Affiliated Hospital, Anhui Medical University, Hefei 230601, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Laboratory Medicine, Second Affiliated Hospital, Anhui Medical University, Hefei 230601, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Laboratory Medicine, Second Affiliated Hospital, Anhui Medical University, Hefei 230601, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Second Affiliated Hospital, Anhui Medical University, Hefei 230601, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Sun
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D006684:HLA-DR Antigens / Q000032:analysis*; D006513:Hepatitis B e Antigens / Q000032:analysis; D019694:Hepatitis B, Chronic / Q000276:immunology*; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D018950:Lipopolysaccharide Receptors / Q000032:analysis*; D022423:Myeloid Cells / Q000276:immunology*
chemical_list: D006684:HLA-DR Antigens; D006513:Hepatitis B e Antigens; D018950:Lipopolysaccharide Receptors; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase