title: Cell adhesion and growth enabled by biomimetic oligopeptide modification of a polydopamine-poly(ethylene oxide) protein repulsive surface.
abstract: Protein-repulsive surfaces modified with ligands for cell adhesion receptors have been widely developed for controlling the cell adhesion and growth in tissue engineering. However, the question of matrix production and deposition by cells on these surfaces has rarely been addressed. In this study, protein-repulsive polydopamine-poly(ethylene oxide) (PDA-PEO) surfaces were functionalized with an RGD-containing peptide (RGD), with a collagen-derived peptide binding fibronectin (Col), or by a combination of these peptides (RGD + Col, ratio 1:1) in concentrations of 90 fmol/cm(2) and 700 fmol/cm(2) for each peptide type. When seeded with vascular endothelial CPAE cells, the PDA-PEO surfaces proved to be completely non-adhesive for cells. On surfaces with lower peptide concentrations and from days 1 to 3 after seeding, cell adhesion and growth was restored practically only on the RGD-modified surface. However, from days 3 to 7, cell adhesion and growth was improved on surfaces modified with Col and with RGD + Col. At higher peptide concentrations, the cell adhesion and growth was markedly improved on all peptide-modified surfaces in both culture intervals. However, the collagen-derived peptide did not increase the expression of fibronectin in the cells. The deposition of fibronectin on the material surface was generally very low and similar on all peptide-modified surfaces. Nevertheless, the RGD + Col surfaces exhibited the highest cell adhesion stability under a dynamic load, which correlated with the highest expression of talin and vinculin in the cells on these surfaces. A combination of RGD + Col therefore seems to be the most promising for surface modification of biomaterials, e.g. vascular prostheses.
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, v.v.i., Videnska 1083, 14220, Prague 4 - Krc, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Musilkova
authors.affiliation: Department of Biomaterials and Bioanalogous Systems, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovsky Sq. 1888/2, 16206, Prague 6, Czech Republic.
authors.forename: Ilya
authors.initials: I
authors.lastname: Kotelnikov
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, v.v.i., Videnska 1083, 14220, Prague 4 - Krc, Czech Republic.
authors.forename: Katarina
authors.initials: K
authors.lastname: Novotna
authors.affiliation: Department of Chemistry and Physics of Surfaces and Biointerfaces, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovsky Sq. 1888/2, 16206, Prague 6, Czech Republic.
authors.forename: Ognen
authors.initials: O
authors.lastname: Pop-Georgievski
authors.affiliation: Department of Biomaterials and Bioanalogous Systems, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovsky Sq. 1888/2, 16206, Prague 6, Czech Republic.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Rypacek
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, v.v.i., Videnska 1083, 14220, Prague 4 - Krc, Czech Republic. lucy@biomed.cas.cz.
authors.forename: Lucie
authors.initials: L
authors.lastname: Bacakova
authors.affiliation: Department of Biomaterials and Bioanalogous Systems, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovsky Sq. 1888/2, 16206, Prague 6, Czech Republic. proks@imc.cas.cz.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Proks
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D032701:Biomimetics; D002448:Cell Adhesion; D002478:Cells, Cultured; D005353:Fibronectins / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D016608:Talin / Q000235:genetics; D016596:Vinculin / Q000235:genetics
chemical_list: D005353:Fibronectins; D007211:Indoles; D009842:Oligopeptides; D011108:Polymers; D016608:Talin; C568283:polydopamine; D016596:Vinculin; D011092:Polyethylene Glycols