title: Pepsin-solubilised collagen (PSC) from Red Sea cucumber (Stichopus japonicus) regulates cell cycle and the fibronectin synthesis in HaCaT cell migration.
abstract: Pepsin-solubilised collagen (PSC) from Red Sea cucumber (Stichopus japonicus) was studied with respect to its wound-healing effects on a human keratinocyte (HaCaT) cell line. Disaggregated collagen fibres were treated with 0.1M NaOH for 24h and digested with pepsin for 72h to reach maximum yield of 26.6%. The results of an in vitro wound-healing test showed that migration of HaCaT cells was 1.5-fold faster on PSC-coated plates than on untreated plates. The migration rate of sea cucumber PSC was similar to that of rat PSC, but five times higher than that of bovine gelatin. HaCaT cells grown on PSC-coated plates revealed increased fibronectin synthesis (6-fold and 3-fold compared to gelatin and rat PSC, respectively). Additionally, sea cucumber PSCs induced HaCaT cell proliferation by decreasing the G1 phase by 5% and maintaining a larger population (8%) of cells in mitosis. Collagen from Red Sea cucumber might be useful as an alternative to mammalian collagen in the nutraceutical and pharmaceutical industries.
authors.affiliation: Jeju Biodiversity Research Institute, Jeju Technopark, Jeju 699-943, Republic of Korea.
authors.forename: Soo-Yeong
authors.initials: SY
authors.lastname: Park
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Lim
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Seogjae
authors.initials: S
authors.lastname: Lee
authors.affiliation: Kwangjin BioTech. Inc., Gyeongsangbuk Gyeongsan 712-838, Republic of Korea.
authors.forename: Hyeong Cheol
authors.initials: HC
authors.lastname: Hwang
authors.affiliation: Faculty of Biotechnology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Somi K
authors.initials: SK
authors.lastname: Cho
authors.affiliation: Department of Biochemistry, School of Medicine, Jeju National University, Jeju 690-756, Republic of Korea; Institute of Medical Science, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: moonjcho@jejunu.ac.kr.
authors.forename: Moonjae
authors.initials: M
authors.lastname: Cho
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002465:Cell Movement; D003094:Collagen / Q000378:metabolism*; D005353:Fibronectins / Q000138:chemical synthesis* / Q000737:chemistry*; D010434:Pepsin A / Q000737:chemistry*; D012616:Sea Cucumbers / Q000737:chemistry*
chemical_list: D005353:Fibronectins; D003094:Collagen; D010434:Pepsin A
keywords: Collagen; HaCaT cells; Proliferation; Red Sea cucumber; Stichopus japonicus; Wound-healing