title: Effect of supplementing a fibrous diet with a xylanase and β-glucanase blend on growth performance, intestinal glucose uptake, and transport-associated gene expression in growing pigs.
abstract: The present study evaluated supplemental carbohydrase effect on performance, intestinal nutrient uptake, and transporter mRNA expressions in growing pigs offered a high-fiber diet manufactured with distillers dried grains with solubles (DDGS). Twenty-four pigs (22.4 ± 0.7 kg BW) were randomly assigned to 1of 3 nutritionally adequate diets (8 pigs per diet) based on corn and soybean meal (SBM) with either 0 (control) or 30% DDGS (high fiber [HF]). The third diet was supplemented with a xylanase and β-glucanase blend (XB) in addition to the 30% DDGS (HF+XB). Parameters determined were ADFI, ADG, G:F, plasma glucose and plasma urea nitrogen (PUN) concentrations, jejunal tissue electrophysiological properties, and mRNA expressions of the sodium-dependent glucose transport 1 (SGLT1) and cationic AA transporter, bo,+AT, in the jejunal and ileal tissues. In addition, mRNA expressions of the short-chain fatty acid transporters, monocarboxylate transporter 1 (MCT1) and sodium-coupled monocarboxylate transporter, and mucin genes were quantified in the ileum. Feed intake, plasma glucose, and jejunal tissue electrophysiological properties were not affected (P > 0.05) by diet. However, control-fed pigs had superior growth rate and feed efficiency and higher PUN (P < 0.05) than HF- and HF+XB-fed pigs. The HF diet increased (P < 0.05) SGLT1 mRNA expression in the jejunum and decreased (P < 0.05) bo,+ mRNA expression in the ileum. The XB supplementation also increased bo,+ mRNA expression in the ileum relative to HF-fed pigs. Additionally, MCT1 mRNA expression was greater (P < 0.05) in the ileum of the HF- and HF+XB-fed pigs. In the present study, XB supplementation influenced nutrient transporter mRNA expression, although it was not accompanied by improved pig performance.
authors.forename: A K
authors.initials: AK
authors.lastname: Agyekum
authors.forename: J S
authors.initials: JS
authors.lastname: Sands
authors.forename: A
authors.initials: A
authors.lastname: Regassa
authors.forename: E
authors.initials: E
authors.lastname: Kiarie
authors.forename: D
authors.initials: D
authors.lastname: Weihrauch
authors.forename: W K
authors.initials: WK
authors.lastname: Kim
authors.forename: C M
authors.initials: CM
authors.lastname: Nyachoti
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001692:Biological Transport / Q000235:genetics / Q000502:physiology; D001806:Blood Urea Nitrogen; D004032:Diet / Q000662:veterinary*; D004043:Dietary Fiber; D019587:Dietary Supplements; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism*; D006026:Glycoside Hydrolases; D007082:Ileum; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000254:growth & development; D013025:Glycine max; D013552:Swine / Q000502:physiology*; D003313:Zea mays / Q000254:growth & development
chemical_list: D004043:Dietary Fiber; D006026:Glycoside Hydrolases; C019822:carbohydrase; D005947:Glucose