title: Melanin Biosynthesis Inhibition Effects of Ginsenoside Rb2 Isolated from Panax ginseng Berry.
abstract: Ginsenoside Rb2 (Gin-Rb2) was purified from the fruit extract of Panax ginseng. Its chemical structure was measured by spectroscopic analysis, including HR-FAB-MS, (1)H-NMR, and IR spectroscopy. Gin-Rb2 decreased potent melanogenesis in melan-a cells, with 23.4% at 80 μM without cytotoxicity. Gin-Rb2 also decreased tyrosinase and MITF protein expression in melan-a cells. Furthermore, Gin-Rb2 presented inhibition of the body pigmentation in the zebrafish in vivo system and reduced melanin contents and tyrosinase activity. These results show that Gin-Rb2 isolated from P. ginseng may be an effective skin-whitening agent via the in vitro and in vivo systems.
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea.
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Lee
authors.affiliation: HK BIO, Business Incubator, Daegu Haany University, Gyeongsan, Gyeongbuk 38578, Republic of Korea.
authors.forename: Yong Tae
authors.initials: YT
authors.lastname: Jeong
authors.affiliation: Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resources, SangJu-si 37242, Republic of Korea.
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Jeong
authors.affiliation: Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
authors.forename: Mi Kyoung
authors.initials: MK
authors.lastname: Lee
authors.affiliation: Research & Development Center, GFC Co., Ltd., Suwon 16690, Republic of Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Min
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea.
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea.
authors.forename: Geum Soog
authors.initials: GS
authors.lastname: Kim
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea.
authors.forename: Seung Eun
authors.initials: SE
authors.lastname: Lee
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea.
authors.forename: Young Sup
authors.initials: YS
authors.lastname: Ahn
authors.affiliation: Research & Development Center, GFC Co., Ltd., Suwon 16690, Republic of Korea.
authors.forename: Hee Cheol
authors.initials: HC
authors.lastname: Kang
authors.affiliation: College of Herbal Bio-industry, Daegu Haany University, Gyeongsan 42158, Republic of Korea.
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D036145:Ginsenosides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008543:Melanins / Q000032:analysis / Q000037:antagonists & inhibitors*; D008544:Melanocytes / Q000187:drug effects*; D014442:Monophenol Monooxygenase / Q000032:analysis / Q000037:antagonists & inhibitors; D005894:Panax / Q000737:chemistry*; D013057:Spectrum Analysis; D015027:Zebrafish
chemical_list: D036145:Ginsenosides; D008543:Melanins; C048026:ginsenoside Rb2; D014442:Monophenol Monooxygenase
keywords: Panax ginseng; ginsenoside Rb2; melan-a cell; melanin biosysnthesis; zebrafish